KLHL35 cDNA ORF clone, Bos taurus(cattle)

The following KLHL35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KLHL35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa36310 NM_001192324.1
Latest version!
Bos taurus kelch like family member 35 (KLHL35), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OBa26599 XM_024975458.1
Latest version!
Bos taurus kelch like family member 35 (KLHL35), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OBa54473 XM_024975457.1
Latest version!
Bos taurus kelch like family member 35 (KLHL35), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OBa54474 XM_015474789.1
Latest version!
Bos taurus kelch like family member 35 (KLHL35), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OBa92744 XM_015474790.1
Latest version!
Bos taurus kelch like family member 35 (KLHL35), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa36310
    Clone ID Related Accession (Same CDS sequence) NM_001192324.1
    Accession Version NM_001192324.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product kelch-like protein 35
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On Jul 17, 2010 this sequence version replaced XM_590044.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGAAGG GCCCCGTGTT GGAGGAGTCG GAGTGGGGCT CCGAGGCGCC ATGCGCGGGG 
    TCCTGCCACG CGCAGCGTGT CCTGCAGACC CTGAACGCAT ATCGGCGGAG CGGCATCCTC
    ACCGACGTGG TGCTGCGAGC CGGTGGCCAC GACTTCCCGT GCCACCGCGC GGCGCTTAGC
    GCGGGCAGTG CCTACTTCCG CAGCTTGTTC GCAGCCGGGA GGCCAGAGCG TGGCCTGGCC
    GTGGTGCCCG TGGTGCCCGA GGCGCCAGGC CCCGCCGGGA CAGCGGTGGC CACGGCCCTG
    GCCGTAGTGC TCGACTACGT GTACTGCGCG GGCGTGCGGT TGCGCGCGGA GGACGAGGCG
    GCCGCGGTGC TGGCGCTGGC TGAGCGGCTG GGCGTGGCGG GCCTGCGTGA GGCCTGCGCG
    CGCTTCCTCG AGGGTCGCCT GCGCGCCGCT AACAGCCTGG CGCTGCGCCG TGTGGCGGCT
    GCCTTCTCCC TCGCCCCTCT GGCTGAGCGC TGCAGCCGCG TGCTGCGCCA GGCGTTCGCC
    GAGGTGGCGC GCCACGCTGA CTTCTTGGAG CTGACGCCTG ACGAGGTGGC AGCGCTGCTG
    GCAGACCCGG CGCTGGGCGT GGCGCACGAG GAGGCCGTGT TCGAGGCGGC CATGCGCTGG
    GTGCGCCACG ACGCGCCGGC CCGCCGCGGC CAGCTGCGGC GCCTGCTGGA GCACGTGCGA
    CTGCCCCTTT TGGCGCCCGC CTACTTCCTG GAGAAGGTGG AGGCCGATGA GTTGCTGCAG
    GCGTGCAGCG AGTGCCGCCC GCTGCTGCTA GAGGCCCGCG CCTGTTTCAT CCTGGGCCGC
    GAGTCTGGCG CACTTCGAGC CCGGCCGCGG AGATTCATGG ACCTAGCTGA AATGATCGTG
    GTCATCGGTG GTTGTGACCG AAAGGGGCTC CTGAAGCTGC CCTTCACCGA CGCCTACCAT
    CCAGACAGCC AGCGCTGGAC CCCGCTGCCC AGCTTGCCCG GCTATGCTCG CTCGGAGTTC
    GCAACGTGTA CTCTCCGCAA TGACATCTAT GTTTCTGGCG GTCACATCAA CAGCCGTGAT
    GTCTGGATGT TCAGCTCCCA TCTGCACACC TGGATCAAGG TGGCCTCGCT GCACAGGGGC
    AGGTGGAGAC ACAAGATGGC GGTGGTGCAG GGGCAGCTGT TCGTTGTGGG CGGCTTCGAT
    GGCCTGAGGC GACTGCGCAG CGTGGAACGC TACGATCCCT TCTCCAACAC CTGGGCGTCG
    GCGGCCCCGC TCCTCGAGGC AGTAAGCTCA GCCGCTGTGG CCCCCTGCGC CGGCCGGCTC
    TACGTCATCG GGGGCGCTGG GCAGGATGGC GTCAGCACCA ACAAGGTGCA GTGCTTTGAT
    TCCAAGGAGG ACCGGTGGAG CCTGCGGTCA CCGGCTCCCT TCTCACAGCG GTGCCTTGAC
    GCTGTCTCCC TGGATAACAC CATCTATGTG GTTGGCGGCC TTATGAGCAA AATCTTCACC
    TACGACCCTG GCACAGATGT CTGGGGGGAG GCAGCTGTCC TCCCCAGCCC TGTGGAGAGC
    TGTGGGGTGA CTGTGTGTGA TGGGAAAGTC CACATCCTCG GCGGGCGGGA TGATCACGGG
    GAAAGCACCG ACAGGGTCTT CACCTTTGAC CCCAGCAGTG GGCAGGTGGA GGCCCAACCG
    TCCCTGCAGC GCTGCACGAG CTCCCATGGC TGCGTCACCA TCGTCCAGAG CCGGGGCAGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179253.1
    CDS1..1743
    Translation

    Target ORF information:

    RefSeq Version NM_001192324.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kelch like family member 35 (KLHL35), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192324.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGAAGG GCCCCGTGTT GGAGGAGTCG GAGTGGGGCT CCGAGGCGCC ATGCGCGGGG 
    TCCTGCCACG CGCAGCGTGT CCTGCAGACC CTGAACGCAT ATCGGCGGAG CGGCATCCTC
    ACCGACGTGG TGCTGCGAGC CGGTGGCCAC GACTTCCCGT GCCACCGCGC GGCGCTTAGC
    GCGGGCAGTG CCTACTTCCG CAGCTTGTTC GCAGCCGGGA GGCCAGAGCG TGGCCTGGCC
    GTGGTGCCCG TGGTGCCCGA GGCGCCAGGC CCCGCCGGGA CAGCGGTGGC CACGGCCCTG
    GCCGTAGTGC TCGACTACGT GTACTGCGCG GGCGTGCGGT TGCGCGCGGA GGACGAGGCG
    GCCGCGGTGC TGGCGCTGGC TGAGCGGCTG GGCGTGGCGG GCCTGCGTGA GGCCTGCGCG
    CGCTTCCTCG AGGGTCGCCT GCGCGCCGCT AACAGCCTGG CGCTGCGCCG TGTGGCGGCT
    GCCTTCTCCC TCGCCCCTCT GGCTGAGCGC TGCAGCCGCG TGCTGCGCCA GGCGTTCGCC
    GAGGTGGCGC GCCACGCTGA CTTCTTGGAG CTGACGCCTG ACGAGGTGGC AGCGCTGCTG
    GCAGACCCGG CGCTGGGCGT GGCGCACGAG GAGGCCGTGT TCGAGGCGGC CATGCGCTGG
    GTGCGCCACG ACGCGCCGGC CCGCCGCGGC CAGCTGCGGC GCCTGCTGGA GCACGTGCGA
    CTGCCCCTTT TGGCGCCCGC CTACTTCCTG GAGAAGGTGG AGGCCGATGA GTTGCTGCAG
    GCGTGCAGCG AGTGCCGCCC GCTGCTGCTA GAGGCCCGCG CCTGTTTCAT CCTGGGCCGC
    GAGTCTGGCG CACTTCGAGC CCGGCCGCGG AGATTCATGG ACCTAGCTGA AATGATCGTG
    GTCATCGGTG GTTGTGACCG AAAGGGGCTC CTGAAGCTGC CCTTCACCGA CGCCTACCAT
    CCAGACAGCC AGCGCTGGAC CCCGCTGCCC AGCTTGCCCG GCTATGCTCG CTCGGAGTTC
    GCAACGTGTA CTCTCCGCAA TGACATCTAT GTTTCTGGCG GTCACATCAA CAGCCGTGAT
    GTCTGGATGT TCAGCTCCCA TCTGCACACC TGGATCAAGG TGGCCTCGCT GCACAGGGGC
    AGGTGGAGAC ACAAGATGGC GGTGGTGCAG GGGCAGCTGT TCGTTGTGGG CGGCTTCGAT
    GGCCTGAGGC GACTGCGCAG CGTGGAACGC TACGATCCCT TCTCCAACAC CTGGGCGTCG
    GCGGCCCCGC TCCTCGAGGC AGTAAGCTCA GCCGCTGTGG CCCCCTGCGC CGGCCGGCTC
    TACGTCATCG GGGGCGCTGG GCAGGATGGC GTCAGCACCA ACAAGGTGCA GTGCTTTGAT
    TCCAAGGAGG ACCGGTGGAG CCTGCGGTCA CCGGCTCCCT TCTCACAGCG GTGCCTTGAC
    GCTGTCTCCC TGGATAACAC CATCTATGTG GTTGGCGGCC TTATGAGCAA AATCTTCACC
    TACGACCCTG GCACAGATGT CTGGGGGGAG GCAGCTGTCC TCCCCAGCCC TGTGGAGAGC
    TGTGGGGTGA CTGTGTGTGA TGGGAAAGTC CACATCCTCG GCGGGCGGGA TGATCACGGG
    GAAAGCACCG ACAGGGTCTT CACCTTTGAC CCCAGCAGTG GGCAGGTGGA GGCCCAACCG
    TCCCTGCAGC GCTGCACGAG CTCCCATGGC TGCGTCACCA TCGTCCAGAG CCGGGGCAGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26599
    Clone ID Related Accession (Same CDS sequence) XM_024975458.1
    Accession Version XM_024975458.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kelch-like protein 35 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCAGGA TGCTGAAGGG CCCCGTGTTG GAGGAGTCGG AGTGGGGCTC CGAGGCGCCA 
    TGCGCGGGGT CCTGCCACGC GCAGCGTGTC CTGCAGACCC TGAACGCATA TCGGCGGAGC
    GGCATCCTCA CCGACGTGGT GCTGCGAGCC GGTGGCCACG ACTTCCCGTG CCACCGCGCG
    GCGCTTAGCG CGGGCAGTGC CTACTTCCGC AGCTTGTTCG CAGCCGGGAG GCCAGAGCGT
    GGCCTGGCCG TGGTGCCCGT GGTGCCCGAG GCGCCAGGCC CCGCCGGGAC AGCGGTGGCC
    ACGGCCCTGG CCGTAGTGCT CGACTACGTG TACTGCGCGG GCGTGCGGTT GCGCGCGGAG
    GACGAGGCGG CCGCGGTGCT GGCGCTGGCT GAGCGGCTGG GCGTGGCGGG CCTGCGTGAG
    GCCTGCGCGC GCTTCCTCGA GGGTCGCCTG CGCGCCGCTA ACAGCCTGGC GCTGCGCCGT
    GTGGCGGCTG CCTTCTCCCT CGCCCCTCTG GCTGAGCGCT GCAGCCGCGT GCTGCGCCAG
    GCGTTCGCCG AGGTGGCGCG CCACGCTGAC TTCTTGGAGC TGACGCCTGA CGAGGTGGCA
    GCGCTGCTGG CAGACCCGGC GCTGGGCGTG GCGCACGAGG AGGCCGTGTT CGAGGCGGCC
    ATGCGCTGGG TGCGCCACGA CGCGCCGGCC CGCCGCGGCC AGCTGCGGCG CCTGCTGGAG
    CACGTGCGAC TGCCCCTTTT GGCGCCCGCC TACTTCCTGG AGAAGGTGGA GGCCGATGAG
    TTGCTGCAGG CGTGCAGCGA GTGCCGCCCG CTGCTGCTAG AGGCCCGCGC CTGTTTCATC
    CTGGGCCGCG AGTCTGGCGC ACTTCGAGCC CGGCCGCGGA GATTCATGGA CCTAGCTGAA
    ATGATCGTGG TCATCGGTGG TTGTGACCGA AAGGGGCTCC TGAAGCTGCC CTTCACCGAC
    GCCTACCATC CAGACAGCCA GCGCTGGACC CCGCTGCCCA GCTTGCCCGG CTATGCTCGC
    TCGGAGTTCG CAACGTGTAC TCTCCGCAAT GACATCTATG TTTCTGGCGG TCACATCAAC
    AGCCGTGATG TCTGGATGTT CAGCTCCCAT CTGCACACCT GGATCAAGGT GGCCTCGCTG
    CACAGGGGCA GGTGGAGACA CAAGATGGCG GTGGTGCAGG GGCAGCTGTT CGTTGTGGGC
    GGCTTCGATG GCCTGAGGCG ACTGCGCAGC GTGGAACGCT ACGATCCCTT CTCCAACACC
    TGGGCGTCGG CGGCCCCGCT CCTCGAGGCA GTAAGCTCAG CCGCTGTGGC CCCCTGCGCC
    GGCCGGCTCT ACGTCATCGG GGGCGCTGGG CAGGATGGCG TCAGCACCAA CAAGGTGCAG
    TGCTTTGATT CCAAGGAGGA CCGGTGGAGC CTGCGGTCAC CGGCTCCCTT CTCACAGCGG
    TGCCTTGACG CTGTCTCCCT GGATAACACC ATCTATGTGG TTGGCGGCCT TATGAGCAAA
    ATCTTCACCT ACGACCCTGG CACAGATGTC TGGGGGGAGG CAGCTGTCCT CCCCAGCCCT
    GTGGAGAGCT GTGGGGTGAC TGTGTGTGAT GGGAAAGTCC ACATCCTCGG CGGGCGGGAT
    GATCACGGGG AAAGCACCGA CAGGGTCTTC ACCTTTGACC CCAGCAGTGG GCAGGTGGAG
    GCCCAACCGT CCCTGCAGCG CTGCACGAGC TCCCATGGCT GCGTCACCAT CGTCCAGAGC
    CGGGGCAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831226.1
    CDS2217..3968
    Translation

    Target ORF information:

    RefSeq Version XM_024975458.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kelch like family member 35 (KLHL35), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCAGGA TGCTGAAGGG CCCCGTGTTG GAGGAGTCGG AGTGGGGCTC CGAGGCGCCA 
    TGCGCGGGGT CCTGCCACGC GCAGCGTGTC CTGCAGACCC TGAACGCATA TCGGCGGAGC
    GGCATCCTCA CCGACGTGGT GCTGCGAGCC GGTGGCCACG ACTTCCCGTG CCACCGCGCG
    GCGCTTAGCG CGGGCAGTGC CTACTTCCGC AGCTTGTTCG CAGCCGGGAG GCCAGAGCGT
    GGCCTGGCCG TGGTGCCCGT GGTGCCCGAG GCGCCAGGCC CCGCCGGGAC AGCGGTGGCC
    ACGGCCCTGG CCGTAGTGCT CGACTACGTG TACTGCGCGG GCGTGCGGTT GCGCGCGGAG
    GACGAGGCGG CCGCGGTGCT GGCGCTGGCT GAGCGGCTGG GCGTGGCGGG CCTGCGTGAG
    GCCTGCGCGC GCTTCCTCGA GGGTCGCCTG CGCGCCGCTA ACAGCCTGGC GCTGCGCCGT
    GTGGCGGCTG CCTTCTCCCT CGCCCCTCTG GCTGAGCGCT GCAGCCGCGT GCTGCGCCAG
    GCGTTCGCCG AGGTGGCGCG CCACGCTGAC TTCTTGGAGC TGACGCCTGA CGAGGTGGCA
    GCGCTGCTGG CAGACCCGGC GCTGGGCGTG GCGCACGAGG AGGCCGTGTT CGAGGCGGCC
    ATGCGCTGGG TGCGCCACGA CGCGCCGGCC CGCCGCGGCC AGCTGCGGCG CCTGCTGGAG
    CACGTGCGAC TGCCCCTTTT GGCGCCCGCC TACTTCCTGG AGAAGGTGGA GGCCGATGAG
    TTGCTGCAGG CGTGCAGCGA GTGCCGCCCG CTGCTGCTAG AGGCCCGCGC CTGTTTCATC
    CTGGGCCGCG AGTCTGGCGC ACTTCGAGCC CGGCCGCGGA GATTCATGGA CCTAGCTGAA
    ATGATCGTGG TCATCGGTGG TTGTGACCGA AAGGGGCTCC TGAAGCTGCC CTTCACCGAC
    GCCTACCATC CAGACAGCCA GCGCTGGACC CCGCTGCCCA GCTTGCCCGG CTATGCTCGC
    TCGGAGTTCG CAACGTGTAC TCTCCGCAAT GACATCTATG TTTCTGGCGG TCACATCAAC
    AGCCGTGATG TCTGGATGTT CAGCTCCCAT CTGCACACCT GGATCAAGGT GGCCTCGCTG
    CACAGGGGCA GGTGGAGACA CAAGATGGCG GTGGTGCAGG GGCAGCTGTT CGTTGTGGGC
    GGCTTCGATG GCCTGAGGCG ACTGCGCAGC GTGGAACGCT ACGATCCCTT CTCCAACACC
    TGGGCGTCGG CGGCCCCGCT CCTCGAGGCA GTAAGCTCAG CCGCTGTGGC CCCCTGCGCC
    GGCCGGCTCT ACGTCATCGG GGGCGCTGGG CAGGATGGCG TCAGCACCAA CAAGGTGCAG
    TGCTTTGATT CCAAGGAGGA CCGGTGGAGC CTGCGGTCAC CGGCTCCCTT CTCACAGCGG
    TGCCTTGACG CTGTCTCCCT GGATAACACC ATCTATGTGG TTGGCGGCCT TATGAGCAAA
    ATCTTCACCT ACGACCCTGG CACAGATGTC TGGGGGGAGG CAGCTGTCCT CCCCAGCCCT
    GTGGAGAGCT GTGGGGTGAC TGTGTGTGAT GGGAAAGTCC ACATCCTCGG CGGGCGGGAT
    GATCACGGGG AAAGCACCGA CAGGGTCTTC ACCTTTGACC CCAGCAGTGG GCAGGTGGAG
    GCCCAACCGT CCCTGCAGCG CTGCACGAGC TCCCATGGCT GCGTCACCAT CGTCCAGAGC
    CGGGGCAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54473
    Clone ID Related Accession (Same CDS sequence) XM_024975457.1
    Accession Version XM_024975457.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kelch-like protein 35 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCAGGA TGCTGAAGGG CCCCGTGTTG GAGGAGTCGG AGTGGGGCTC CGAGGCGCCA 
    TGCGCGGGGT CCTGCCACGC GCAGCGTGTC CTGCAGACCC TGAACGCATA TCGGCGGAGC
    GGCATCCTCA CCGACGTGGT GCTGCGAGCC GGTGGCCACG ACTTCCCGTG CCACCGCGCG
    GCGCTTAGCG CGGGCAGTGC CTACTTCCGC AGCTTGTTCG CAGCCGGGAG GCCAGAGCGT
    GGCCTGGCCG TGGTGCCCGT GGTGCCCGAG GCGCCAGGCC CCGCCGGGAC AGCGGTGGCC
    ACGGCCCTGG CCGTAGTGCT CGACTACGTG TACTGCGCGG GCGTGCGGTT GCGCGCGGAG
    GACGAGGCGG CCGCGGTGCT GGCGCTGGCT GAGCGGCTGG GCGTGGCGGG CCTGCGTGAG
    GCCTGCGCGC GCTTCCTCGA GGGTCGCCTG CGCGCCGCTA ACAGCCTGGC GCTGCGCCGT
    GTGGCGGCTG CCTTCTCCCT CGCCCCTCTG GCTGAGCGCT GCAGCCGCGT GCTGCGCCAG
    GCGTTCGCCG AGGTGGCGCG CCACGCTGAC TTCTTGGAGC TGACGCCTGA CGAGGTGGCA
    GCGCTGCTGG CAGACCCGGC GCTGGGCGTG GCGCACGAGG AGGCCGTGTT CGAGGCGGCC
    ATGCGCTGGG TGCGCCACGA CGCGCCGGCC CGCCGCGGCC AGCTGCGGCG CCTGCTGGAG
    CACGTGCGAC TGCCCCTTTT GGCGCCCGCC TACTTCCTGG AGAAGGTGGA GGCCGATGAG
    TTGCTGCAGG CGTGCAGCGA GTGCCGCCCG CTGCTGCTAG AGGCCCGCGC CTGTTTCATC
    CTGGGCCGCG AGTCTGGCGC ACTTCGAGCC CGGCCGCGGA GATTCATGGA CCTAGCTGAA
    ATGATCGTGG TCATCGGTGG TTGTGACCGA AAGGGGCTCC TGAAGCTGCC CTTCACCGAC
    GCCTACCATC CAGACAGCCA GCGCTGGACC CCGCTGCCCA GCTTGCCCGG CTATGCTCGC
    TCGGAGTTCG CAACGTGTAC TCTCCGCAAT GACATCTATG TTTCTGGCGG TCACATCAAC
    AGCCGTGATG TCTGGATGTT CAGCTCCCAT CTGCACACCT GGATCAAGGT GGCCTCGCTG
    CACAGGGGCA GGTGGAGACA CAAGATGGCG GTGGTGCAGG GGCAGCTGTT CGTTGTGGGC
    GGCTTCGATG GCCTGAGGCG ACTGCGCAGC GTGGAACGCT ACGATCCCTT CTCCAACACC
    TGGGCGTCGG CGGCCCCGCT CCTCGAGGCA GTAAGCTCAG CCGCTGTGGC CCCCTGCGCC
    GGCCGGCTCT ACGTCATCGG GGGCGCTGGG CAGGATGGCG TCAGCACCAA CAAGGTGCAG
    TGCTTTGATT CCAAGGAGGA CCGGTGGAGC CTGCGGTCAC CGGCTCCCTT CTCACAGCGG
    TGCCTTGACG CTGTCTCCCT GGATAACACC ATCTATGTGG TTGGCGGCCT TATGAGCAAA
    ATCTTCACCT ACGACCCTGG CACAGATGTC TGGGGGGAGG CAGCTGTCCT CCCCAGCCCT
    GTGGAGAGCT GTGGGGTGAC TGTGTGTGAT GGGAAAGTCC ACATCCTCGG CGGGCGGGAT
    GATCACGGGG AAAGCACCGA CAGGGTCTTC ACCTTTGACC CCAGCAGTGG GCAGGTGGAG
    GCCCAACCGT CCCTGCAGCG CTGCACGAGC TCCCATGGCT GCGTCACCAT CGTCCAGAGC
    CGGGGCAGAA ATAAAACCTT CTTCAAAGGT CGAGTCTGTG TTCCAGCTCA AGCCTCCTTT
    GCCTGGAGAA GTAATGCCCA ATATCTGCTC CCTGCTATGT CTCTTTACAA AGCACTTCAG
    CACTTCATGT TACTTCATTT ACCTTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831225.1
    CDS2217..4106
    Translation

    Target ORF information:

    RefSeq Version XM_024975457.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kelch like family member 35 (KLHL35), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCAGGA TGCTGAAGGG CCCCGTGTTG GAGGAGTCGG AGTGGGGCTC CGAGGCGCCA 
    TGCGCGGGGT CCTGCCACGC GCAGCGTGTC CTGCAGACCC TGAACGCATA TCGGCGGAGC
    GGCATCCTCA CCGACGTGGT GCTGCGAGCC GGTGGCCACG ACTTCCCGTG CCACCGCGCG
    GCGCTTAGCG CGGGCAGTGC CTACTTCCGC AGCTTGTTCG CAGCCGGGAG GCCAGAGCGT
    GGCCTGGCCG TGGTGCCCGT GGTGCCCGAG GCGCCAGGCC CCGCCGGGAC AGCGGTGGCC
    ACGGCCCTGG CCGTAGTGCT CGACTACGTG TACTGCGCGG GCGTGCGGTT GCGCGCGGAG
    GACGAGGCGG CCGCGGTGCT GGCGCTGGCT GAGCGGCTGG GCGTGGCGGG CCTGCGTGAG
    GCCTGCGCGC GCTTCCTCGA GGGTCGCCTG CGCGCCGCTA ACAGCCTGGC GCTGCGCCGT
    GTGGCGGCTG CCTTCTCCCT CGCCCCTCTG GCTGAGCGCT GCAGCCGCGT GCTGCGCCAG
    GCGTTCGCCG AGGTGGCGCG CCACGCTGAC TTCTTGGAGC TGACGCCTGA CGAGGTGGCA
    GCGCTGCTGG CAGACCCGGC GCTGGGCGTG GCGCACGAGG AGGCCGTGTT CGAGGCGGCC
    ATGCGCTGGG TGCGCCACGA CGCGCCGGCC CGCCGCGGCC AGCTGCGGCG CCTGCTGGAG
    CACGTGCGAC TGCCCCTTTT GGCGCCCGCC TACTTCCTGG AGAAGGTGGA GGCCGATGAG
    TTGCTGCAGG CGTGCAGCGA GTGCCGCCCG CTGCTGCTAG AGGCCCGCGC CTGTTTCATC
    CTGGGCCGCG AGTCTGGCGC ACTTCGAGCC CGGCCGCGGA GATTCATGGA CCTAGCTGAA
    ATGATCGTGG TCATCGGTGG TTGTGACCGA AAGGGGCTCC TGAAGCTGCC CTTCACCGAC
    GCCTACCATC CAGACAGCCA GCGCTGGACC CCGCTGCCCA GCTTGCCCGG CTATGCTCGC
    TCGGAGTTCG CAACGTGTAC TCTCCGCAAT GACATCTATG TTTCTGGCGG TCACATCAAC
    AGCCGTGATG TCTGGATGTT CAGCTCCCAT CTGCACACCT GGATCAAGGT GGCCTCGCTG
    CACAGGGGCA GGTGGAGACA CAAGATGGCG GTGGTGCAGG GGCAGCTGTT CGTTGTGGGC
    GGCTTCGATG GCCTGAGGCG ACTGCGCAGC GTGGAACGCT ACGATCCCTT CTCCAACACC
    TGGGCGTCGG CGGCCCCGCT CCTCGAGGCA GTAAGCTCAG CCGCTGTGGC CCCCTGCGCC
    GGCCGGCTCT ACGTCATCGG GGGCGCTGGG CAGGATGGCG TCAGCACCAA CAAGGTGCAG
    TGCTTTGATT CCAAGGAGGA CCGGTGGAGC CTGCGGTCAC CGGCTCCCTT CTCACAGCGG
    TGCCTTGACG CTGTCTCCCT GGATAACACC ATCTATGTGG TTGGCGGCCT TATGAGCAAA
    ATCTTCACCT ACGACCCTGG CACAGATGTC TGGGGGGAGG CAGCTGTCCT CCCCAGCCCT
    GTGGAGAGCT GTGGGGTGAC TGTGTGTGAT GGGAAAGTCC ACATCCTCGG CGGGCGGGAT
    GATCACGGGG AAAGCACCGA CAGGGTCTTC ACCTTTGACC CCAGCAGTGG GCAGGTGGAG
    GCCCAACCGT CCCTGCAGCG CTGCACGAGC TCCCATGGCT GCGTCACCAT CGTCCAGAGC
    CGGGGCAGAA ATAAAACCTT CTTCAAAGGT CGAGTCTGTG TTCCAGCTCA AGCCTCCTTT
    GCCTGGAGAA GTAATGCCCA ATATCTGCTC CCTGCTATGT CTCTTTACAA AGCACTTCAG
    CACTTCATGT TACTTCATTT ACCTTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54474
    Clone ID Related Accession (Same CDS sequence) XM_015474789.1
    Accession Version XM_015474789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kelch-like protein 35 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104417.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCTGAAGG GCCCCGTGTT GGAGGAGTCG GAGTGGGGCT CCGAGGCGCC ATGCGCGGGG 
    TCCTGCCACG CGCAGCGTGT CCTGCAGACC CTGAACGCAT ATCGGCGGAG CGGCATCCTC
    ACCGACGTGG TGCTGCGAGC CGGTGGCCAC GACTTCCCGT GCCACCGCGC GGCGCTTAGC
    GCGGGCAGTG CCTACTTCCG CAGCTTGTTC GCAGCCGGGA GGCCAGAGCG TGGCCTGGCC
    GTGGTGCCCG TGGTGCCCGA GGCGCCAGGC CCCGCCGGGA CAGCGGTGGC CACGGCCCTG
    GCCGTAGTGC TCGACTACGT GTACTGCGCG GGCGTGCGGT TGCGCGCGGA GGACGAGGCG
    GCCGCGGTGC TGGCGCTGGC TGAGCGGCTG GGCGTGGCGG GCCTGCGTGA GGCCTGCGCG
    CGCTTCCTCG AGGGTCGCCT GCGCGCCGCT AACAGCCTGG CGCTGCGCCG TGTGGCGGCT
    GCCTTCTCCC TCGCCCCTCT GGCTGAGCGC TGCAGCCGCG TGCTGCGCCA GGCGTTCGCC
    GAGGTGGCGC GCCACGCTGA CTTCTTGGAG CTGACGCCTG ACGAGGTGGC AGCGCTGCTG
    GCAGACCCGG CGCTGGGCGT GGCGCACGAG GAGGCCGTGT TCGAGGCGGC CATGCGCTGG
    GTGCGCCACG ACGCGCCGGC CCGCCGCGGC CAGCTGCGGC GCCTGCTGGA GCACGTGCGA
    CTGCCCCTTT TGGCGCCCGC CTACTTCCTG GAGAAGGTGG AGGCCGATGA GTTGCTGCAG
    GCGTGCAGCG AGTGCCGCCC GCTGCTGCTA GAGGCCCGCG CCTGTTTCAT CCTGGGCCGC
    GAGTCTGGCG CACTTCGAGC CCGGCCGCGG AGATTCATGG ACCTAGCTGA AATGATCGTG
    GTCATCGGTG GTTGTGACCG AAAGGGGCTC CTGAAGCTGC CCTTCACCGA CGCCTACCAT
    CCAGACAGCC AGCGCTGGAC CCCGCTGCCC AGCTTGCCCG GCTATGCTCG CTCGGAGTTC
    GCAACGTGTA CTCTCCGCAA TGACATCTAT GTTTCTGGCG GTCACATCAA CAGCCGTGAT
    GTCTGGATGT TCAGCTCCCA TCTGCACACC TGGATCAAGG TGGCCTCGCT GCACAGGGGC
    AGGTGGAGAC ACAAGATGGC GGTGGTGCAG GGGCAGCTGT TCGTTGTGGG CGGCTTCGAT
    GGCCTGAGGC GACTGCGCAG CGTGGAACGC TACGATCCCT TCTCCAACAC CTGGGCGTCG
    GCGGCCCCGC TCCTCGAGGC AGTAAGCTCA GCCGCTGTGG CCCCCTGCGC CGGCCGGCTC
    TACGTCATCG GGGGCGCTGG GCAGGATGGC GTCAGCACCA ACAAGGTGCA GTGCTTTGAT
    TCCAAGGAGG ACCGGTGGAG CCTGCGGTCA CCGGCTCCCT TCTCACAGCG GTGCCTTGAC
    GCTGTCTCCC TGGATAACAC CATCTATGTG GTTGGCGGCC TTATGAGCAA AATCTTCACC
    TACGACCCTG GCACAGATGT CTGGGGGGAG GCAGCTGTCC TCCCCAGCCC TGTGGAGAGC
    TGTGGGGTGA CTGTGTGTGA TGGGAAAGTC CACATCCTCG GCGGGCGGGA TGATCACGGG
    GAAAGCACCG ACAGGGTCTT CACCTTTGAC CCCAGCAGTG GGCAGGTGGA GGCCCAACCG
    TCCCTGCAGC GCTGCACGAG CTCCCATGGC TGCGTCACCA TCGTCCAGAG CCGGGGCAGA
    AATAAAACCT TCTTCAAAGG TCGAGTCTGT GTTCCAGCTC AAGCCTCCTT TGCCTGGAGA
    AGTAATGCCC AATATCTGCT CCCTGCTATG TCTCTTTACA AAGCACTTCA GCACTTCATG
    TTACTTCATT TACCTTCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330275.1
    CDS2716..4596
    Translation

    Target ORF information:

    RefSeq Version XM_015474789.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kelch like family member 35 (KLHL35), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCTGAAGG GCCCCGTGTT GGAGGAGTCG GAGTGGGGCT CCGAGGCGCC ATGCGCGGGG 
    TCCTGCCACG CGCAGCGTGT CCTGCAGACC CTGAACGCAT ATCGGCGGAG CGGCATCCTC
    ACCGACGTGG TGCTGCGAGC CGGTGGCCAC GACTTCCCGT GCCACCGCGC GGCGCTTAGC
    GCGGGCAGTG CCTACTTCCG CAGCTTGTTC GCAGCCGGGA GGCCAGAGCG TGGCCTGGCC
    GTGGTGCCCG TGGTGCCCGA GGCGCCAGGC CCCGCCGGGA CAGCGGTGGC CACGGCCCTG
    GCCGTAGTGC TCGACTACGT GTACTGCGCG GGCGTGCGGT TGCGCGCGGA GGACGAGGCG
    GCCGCGGTGC TGGCGCTGGC TGAGCGGCTG GGCGTGGCGG GCCTGCGTGA GGCCTGCGCG
    CGCTTCCTCG AGGGTCGCCT GCGCGCCGCT AACAGCCTGG CGCTGCGCCG TGTGGCGGCT
    GCCTTCTCCC TCGCCCCTCT GGCTGAGCGC TGCAGCCGCG TGCTGCGCCA GGCGTTCGCC
    GAGGTGGCGC GCCACGCTGA CTTCTTGGAG CTGACGCCTG ACGAGGTGGC AGCGCTGCTG
    GCAGACCCGG CGCTGGGCGT GGCGCACGAG GAGGCCGTGT TCGAGGCGGC CATGCGCTGG
    GTGCGCCACG ACGCGCCGGC CCGCCGCGGC CAGCTGCGGC GCCTGCTGGA GCACGTGCGA
    CTGCCCCTTT TGGCGCCCGC CTACTTCCTG GAGAAGGTGG AGGCCGATGA GTTGCTGCAG
    GCGTGCAGCG AGTGCCGCCC GCTGCTGCTA GAGGCCCGCG CCTGTTTCAT CCTGGGCCGC
    GAGTCTGGCG CACTTCGAGC CCGGCCGCGG AGATTCATGG ACCTAGCTGA AATGATCGTG
    GTCATCGGTG GTTGTGACCG AAAGGGGCTC CTGAAGCTGC CCTTCACCGA CGCCTACCAT
    CCAGACAGCC AGCGCTGGAC CCCGCTGCCC AGCTTGCCCG GCTATGCTCG CTCGGAGTTC
    GCAACGTGTA CTCTCCGCAA TGACATCTAT GTTTCTGGCG GTCACATCAA CAGCCGTGAT
    GTCTGGATGT TCAGCTCCCA TCTGCACACC TGGATCAAGG TGGCCTCGCT GCACAGGGGC
    AGGTGGAGAC ACAAGATGGC GGTGGTGCAG GGGCAGCTGT TCGTTGTGGG CGGCTTCGAT
    GGCCTGAGGC GACTGCGCAG CGTGGAACGC TACGATCCCT TCTCCAACAC CTGGGCGTCG
    GCGGCCCCGC TCCTCGAGGC AGTAAGCTCA GCCGCTGTGG CCCCCTGCGC CGGCCGGCTC
    TACGTCATCG GGGGCGCTGG GCAGGATGGC GTCAGCACCA ACAAGGTGCA GTGCTTTGAT
    TCCAAGGAGG ACCGGTGGAG CCTGCGGTCA CCGGCTCCCT TCTCACAGCG GTGCCTTGAC
    GCTGTCTCCC TGGATAACAC CATCTATGTG GTTGGCGGCC TTATGAGCAA AATCTTCACC
    TACGACCCTG GCACAGATGT CTGGGGGGAG GCAGCTGTCC TCCCCAGCCC TGTGGAGAGC
    TGTGGGGTGA CTGTGTGTGA TGGGAAAGTC CACATCCTCG GCGGGCGGGA TGATCACGGG
    GAAAGCACCG ACAGGGTCTT CACCTTTGAC CCCAGCAGTG GGCAGGTGGA GGCCCAACCG
    TCCCTGCAGC GCTGCACGAG CTCCCATGGC TGCGTCACCA TCGTCCAGAG CCGGGGCAGA
    AATAAAACCT TCTTCAAAGG TCGAGTCTGT GTTCCAGCTC AAGCCTCCTT TGCCTGGAGA
    AGTAATGCCC AATATCTGCT CCCTGCTATG TCTCTTTACA AAGCACTTCA GCACTTCATG
    TTACTTCATT TACCTTCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92744
    Clone ID Related Accession (Same CDS sequence) XM_015474790.1
    Accession Version XM_015474790.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kelch-like protein 35 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104417.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCTGAAGG GCCCCGTGTT GGAGGAGTCG GAGTGGGGCT CCGAGGCGCC ATGCGCGGGG 
    TCCTGCCACG CGCAGCGTGT CCTGCAGACC CTGAACGCAT ATCGGCGGAG CGGCATCCTC
    ACCGACGTGG TGCTGCGAGC CGGTGGCCAC GACTTCCCGT GCCACCGCGC GGCGCTTAGC
    GCGGGCAGTG CCTACTTCCG CAGCTTGTTC GCAGCCGGGA GGCCAGAGCG TGGCCTGGCC
    GTGGTGCCCG TGGTGCCCGA GGCGCCAGGC CCCGCCGGGA CAGCGGTGGC CACGGCCCTG
    GCCGTAGTGC TCGACTACGT GTACTGCGCG GGCGTGCGGT TGCGCGCGGA GGACGAGGCG
    GCCGCGGTGC TGGCGCTGGC TGAGCGGCTG GGCGTGGCGG GCCTGCGTGA GGCCTGCGCG
    CGCTTCCTCG AGGGTCGCCT GCGCGCCGCT AACAGCCTGG CGCTGCGCCG TGTGGCGGCT
    GCCTTCTCCC TCGCCCCTCT GGCTGAGCGC TGCAGCCGCG TGCTGCGCCA GGCGTTCGCC
    GAGGTGGCGC GCCACGCTGA CTTCTTGGAG CTGACGCCTG ACGAGGTGGC AGCGCTGCTG
    GCAGACCCGG CGCTGGGCGT GGCGCACGAG GAGGCCGTGT TCGAGGCGGC CATGCGCTGG
    GTGCGCCACG ACGCGCCGGC CCGCCGCGGC CAGCTGCGGC GCCTGCTGGA GCACGTGCGA
    CTGCCCCTTT TGGCGCCCGC CTACTTCCTG GAGAAGGTGG AGGCCGATGA GTTGCTGCAG
    GCGTGCAGCG AGTGCCGCCC GCTGCTGCTA GAGGCCCGCG CCTGTTTCAT CCTGGGCCGC
    GAGTCTGGCG CACTTCGAGC CCGGCCGCGG AGATTCATGG ACCTAGCTGA AATGATCGTG
    GTCATCGGTG GTTGTGACCG AAAGGGGCTC CTGAAGCTGC CCTTCACCGA CGCCTACCAT
    CCAGACAGCC AGCGCTGGAC CCCGCTGCCC AGCTTGCCCG GCTATGCTCG CTCGGAGTTC
    GCAACGTGTA CTCTCCGCAA TGACATCTAT GTTTCTGCTG TTCGTTGTGG GCGGCTTCGA
    TGGCCTGAGG CGACTGCGCA GCGTGGAACG CTACGATCCC TTCTCCAACA CCTGGGCGTC
    GGCGGCCCCG CTCCTCGAGG CAGTAAGCTC AGCCGCTGTG GCCCCCTGCG CCGGCCGGCT
    CTACGTCATC GGGGGCGCTG GGCAGGATGG CGTCAGCACC AACAAGGTGG GCCAGCAGAG
    GGCGGGGGAG GCCTGCTGTG TGCCAGGATG GCCTTGTCCG TTTTCTTGAT TTCATCGACA
    ACACTGAGTC ATCCTCCCCA TATTTCAGGC AAGGAAATGA ATTCAGAAAA AGGACCCCAA
    GGTCACACAG TAGGTCTGGA CCTAAATGTT TGGCCTGGTG CTATACGGGT TTCAAAGATT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330276.1
    CDS2723..4165
    Translation

    Target ORF information:

    RefSeq Version XM_015474790.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kelch like family member 35 (KLHL35), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474790.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCTGAAGG GCCCCGTGTT GGAGGAGTCG GAGTGGGGCT CCGAGGCGCC ATGCGCGGGG 
    TCCTGCCACG CGCAGCGTGT CCTGCAGACC CTGAACGCAT ATCGGCGGAG CGGCATCCTC
    ACCGACGTGG TGCTGCGAGC CGGTGGCCAC GACTTCCCGT GCCACCGCGC GGCGCTTAGC
    GCGGGCAGTG CCTACTTCCG CAGCTTGTTC GCAGCCGGGA GGCCAGAGCG TGGCCTGGCC
    GTGGTGCCCG TGGTGCCCGA GGCGCCAGGC CCCGCCGGGA CAGCGGTGGC CACGGCCCTG
    GCCGTAGTGC TCGACTACGT GTACTGCGCG GGCGTGCGGT TGCGCGCGGA GGACGAGGCG
    GCCGCGGTGC TGGCGCTGGC TGAGCGGCTG GGCGTGGCGG GCCTGCGTGA GGCCTGCGCG
    CGCTTCCTCG AGGGTCGCCT GCGCGCCGCT AACAGCCTGG CGCTGCGCCG TGTGGCGGCT
    GCCTTCTCCC TCGCCCCTCT GGCTGAGCGC TGCAGCCGCG TGCTGCGCCA GGCGTTCGCC
    GAGGTGGCGC GCCACGCTGA CTTCTTGGAG CTGACGCCTG ACGAGGTGGC AGCGCTGCTG
    GCAGACCCGG CGCTGGGCGT GGCGCACGAG GAGGCCGTGT TCGAGGCGGC CATGCGCTGG
    GTGCGCCACG ACGCGCCGGC CCGCCGCGGC CAGCTGCGGC GCCTGCTGGA GCACGTGCGA
    CTGCCCCTTT TGGCGCCCGC CTACTTCCTG GAGAAGGTGG AGGCCGATGA GTTGCTGCAG
    GCGTGCAGCG AGTGCCGCCC GCTGCTGCTA GAGGCCCGCG CCTGTTTCAT CCTGGGCCGC
    GAGTCTGGCG CACTTCGAGC CCGGCCGCGG AGATTCATGG ACCTAGCTGA AATGATCGTG
    GTCATCGGTG GTTGTGACCG AAAGGGGCTC CTGAAGCTGC CCTTCACCGA CGCCTACCAT
    CCAGACAGCC AGCGCTGGAC CCCGCTGCCC AGCTTGCCCG GCTATGCTCG CTCGGAGTTC
    GCAACGTGTA CTCTCCGCAA TGACATCTAT GTTTCTGCTG TTCGTTGTGG GCGGCTTCGA
    TGGCCTGAGG CGACTGCGCA GCGTGGAACG CTACGATCCC TTCTCCAACA CCTGGGCGTC
    GGCGGCCCCG CTCCTCGAGG CAGTAAGCTC AGCCGCTGTG GCCCCCTGCG CCGGCCGGCT
    CTACGTCATC GGGGGCGCTG GGCAGGATGG CGTCAGCACC AACAAGGTGG GCCAGCAGAG
    GGCGGGGGAG GCCTGCTGTG TGCCAGGATG GCCTTGTCCG TTTTCTTGAT TTCATCGACA
    ACACTGAGTC ATCCTCCCCA TATTTCAGGC AAGGAAATGA ATTCAGAAAA AGGACCCCAA
    GGTCACACAG TAGGTCTGGA CCTAAATGTT TGGCCTGGTG CTATACGGGT TTCAAAGATT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.