IRS1 cDNA ORF clone, Bos taurus(cattle)

The following IRS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227808 XM_003585773.5
Latest version!
Bos taurus insulin receptor substrate 1 (IRS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa02991 XM_003585773.4
Latest version!
Bos taurus insulin receptor substrate 1 (IRS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa02991 XM_003581871.4
Latest version!
Bos taurus insulin receptor substrate 1 (IRS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227808
    Clone ID Related Accession (Same CDS sequence) XM_003585773.5
    Accession Version XM_003585773.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product insulin receptor substrate 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003585773.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC
    AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC
    CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC
    GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG
    GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG
    GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG
    AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC
    CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG
    CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC
    TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA
    GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT
    GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC
    AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG
    GAGCTAAGCA ACTACATCTG CATGGGAGGC AAAGGGGCCT CCACCCTCAC TGCCCCCAAT
    GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG
    GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG
    TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG
    TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA
    GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC
    TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC
    CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC
    CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG
    TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC
    AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA
    AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC
    GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC
    TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC
    GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG
    GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC
    ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC
    TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT
    CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG
    CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG
    CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT
    GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC
    AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT
    ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG
    CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA
    GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC
    GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG
    GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT
    GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG
    GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA
    GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT
    ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG
    GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC
    TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA
    GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC
    AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG
    GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT
    CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003585821.1
    CDS1142..4855
    Translation

    Target ORF information:

    RefSeq Version XM_003585773.5
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor substrate 1 (IRS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003585773.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC
    AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC
    CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC
    GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG
    GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG
    GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG
    AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC
    CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG
    CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC
    TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA
    GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT
    GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC
    AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG
    GAGCTAAGCA ACTACATCTG CATGGGAGGC AAAGGGGCCT CCACCCTCAC TGCCCCCAAT
    GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG
    GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG
    TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG
    TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA
    GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC
    TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC
    CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC
    CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG
    TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC
    AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA
    AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC
    GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC
    TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC
    GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG
    GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC
    ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC
    TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT
    CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG
    CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG
    CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT
    GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC
    AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT
    ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG
    CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA
    GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC
    GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG
    GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT
    GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG
    GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA
    GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT
    ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG
    GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC
    TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA
    GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC
    AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG
    GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT
    CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02991
    Clone ID Related Accession (Same CDS sequence) XM_003581871.4 , XM_003585773.4
    Accession Version XM_003581871.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor substrate 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003581871.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC TAGCGCCCCC
    AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC
    CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC
    GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG
    GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG
    GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG
    AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC
    CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG
    CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC
    TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA
    GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT
    GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC
    AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG
    GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT
    GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG
    GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG
    TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG
    TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA
    GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC
    TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC
    CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC
    CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG
    TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC
    AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA
    AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC
    GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC
    TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC
    GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG
    GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC
    ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC
    TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT
    CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG
    CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG
    CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT
    GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC
    AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT
    ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG
    CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA
    GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC
    GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG
    GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT
    GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG
    GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA
    GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT
    ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG
    GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC
    TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA
    GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC
    AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG
    GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT
    CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003581919.1
    CDS345..4058
    Translation

    Target ORF information:

    RefSeq Version XM_003581871.4
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor substrate 1 (IRS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003581871.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC TAGCGCCCCC
    AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC
    CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC
    GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG
    GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG
    GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG
    AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC
    CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG
    CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC
    TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA
    GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT
    GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC
    AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG
    GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT
    GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG
    GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG
    TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG
    TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA
    GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC
    TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC
    CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC
    CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG
    TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC
    AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA
    AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC
    GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC
    TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC
    GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG
    GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC
    ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC
    TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT
    CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG
    CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG
    CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT
    GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC
    AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT
    ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG
    CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA
    GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC
    GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG
    GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT
    GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG
    GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA
    GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT
    ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG
    GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC
    TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA
    GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC
    AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG
    GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT
    CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02991
    Clone ID Related Accession (Same CDS sequence) XM_003581871.4 , XM_003585773.4
    Accession Version XM_003585773.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor substrate 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003585773.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC TAGCGCCCCC
    AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC
    CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC
    GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG
    GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG
    GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG
    AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC
    CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG
    CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC
    TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA
    GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT
    GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC
    AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG
    GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT
    GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG
    GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG
    TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG
    TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA
    GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC
    TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC
    CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC
    CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG
    TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC
    AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA
    AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC
    GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC
    TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC
    GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG
    GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC
    ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC
    TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT
    CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG
    CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG
    CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT
    GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC
    AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT
    ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG
    CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA
    GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC
    GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG
    GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT
    GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG
    GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA
    GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT
    ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG
    GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC
    TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA
    GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC
    AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG
    GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT
    CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003585821.1
    CDS345..4058
    Translation

    Target ORF information:

    RefSeq Version XM_003585773.4
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor substrate 1 (IRS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003585773.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC TAGCGCCCCC
    AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC
    CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC
    GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG
    GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG
    GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG
    AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC
    CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG
    CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC
    TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA
    GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT
    GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC
    AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG
    GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT
    GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG
    GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG
    TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG
    TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA
    GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC
    TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC
    CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC
    CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG
    TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC
    AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA
    AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC
    GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC
    TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC
    GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG
    GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC
    ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC
    TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT
    CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG
    CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG
    CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT
    GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC
    AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT
    ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG
    CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA
    GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC
    GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG
    GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT
    GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG
    GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA
    GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT
    ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG
    GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC
    TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA
    GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC
    AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG
    GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT
    CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.