irs1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following irs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa10305 XM_002937088.4
Latest version!
Xenopus tropicalis insulin receptor substrate 1 (irs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa10306 XM_012963449.3
Latest version!
Xenopus tropicalis insulin receptor substrate 1 (irs1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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OXa10305 XM_002937088.3 Xenopus tropicalis insulin receptor substrate 1 (irs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa10306 XM_012963449.2 Xenopus tropicalis insulin receptor substrate 1 (irs1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa10305
    Clone ID Related Accession (Same CDS sequence) XM_002937088.3 , XM_002937088.4
    Accession Version XM_002937088.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product insulin receptor substrate 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937088.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTAGCC CCACAGATCC ACAGGCTCAG GAGAACTTTT CTGATGTCAG AAAGGTGGGC 
    TATCTAAGGA AACCTAAGAG CATGCACAAG AGGTTTTTTG TGCTCAGGTC TGCCAGTGAG
    TCAGGTTTGG CCCGTTTGGA GTATTATGAA AATGAGAAAA AGTGGAGGCA TAAGTCTGGG
    GCCCCAAAGA GGTCAATCCC TCTTGAGAGC TGCTTTAATA TTAACAAGAG GGCTGATTCA
    AAAAACAAAC ATTTGGTTGC TTTGTACACA AAGGAGGAAT GCTTTGCAAT TGCTGCTGAA
    TGTGAACAAG AACAGGATGG TTGGTATCAG GCTCTAGTGG ATCTTCACAA CCGTGGTAAG
    ACCCACCACC AACACCATAA CCATGACGGT GCAACCAATG GTGTACATGA TGGTTTAAAT
    GGAGATGACG TTTATGGGGA GGTTACTCCT CCAGGACTGG CCTTCAAAGA GGTATGGCAG
    GTGATCATGA AGCCCAAGGG TTTAGGGCAA CTTAAGAATT TAGTTGGTAT TTACCGCCTG
    TGCCTCACCA ACCGAACAAT AAGTTTGGTA AAACTGAACT CTGATGCAGC CGCTGTGGTA
    TTGCAACTTA TGAATATAAG GAGATGTGGG CATTCAGAAA ATTTTTTCTT TATAGAAGTG
    GGTCGTTCAG CAGTCACCGG AGCTGGTGAA TTCTGGATGC AAGTGGATGA TTCAGTAGTT
    GCTCAAAACA TGCATGAAAC CATACTAGAA GCTATGAAAG CTCTGAGCGA TGAATTTCGG
    CCTCGGAGTA AGAGTCAGTC TTCATCTAAC TGCTCTAATC CTATATCTGT ACCTCTTAGG
    AGGCATCACC TTAATCATCC ACCACCCAGC CAGGTAGGAC TAAATAGAAG AGCTCGTACA
    GAGAGTGTTA CTGCCACATC ACCTGCAGGG GGTGTGGCAA AGCATGGATC ATCCTCCTCT
    TTCAGAGTCC GGGCATCTAG TGATGGAGAA GGAACAATGT CAAGGCCAGC CTCCATGGAA
    GGGAGCCCAG TAAGTCCCAG TGCTAGCAGA GCTCAGTCGC ACCGGCATCG GGGAAGTTCT
    CGACTTCACC CGCCTCTTAA CCATAGTCGG TCTATACCAA TGCCAGCAAC TCGCTGCTCT
    CCATCGGCTA CCAGCCCTGT CAGCTTGTCC TCCAGTAGTA CCAGTGGACA TGGTTCCACT
    TCTGATTGCA TGTGCCCTCG AAGATCCAGT GCCTCTGTTT CTGGTTCACC CAGTGATGGT
    GGTTTTATTT CTTCAGATGA ATATGGGTCT AGCCCTTGTG ACTTTAGAAG TTCTTTTCGC
    AGTGTAACCC CAGATTCTTT GGGCCACACT CCACCAGCTA GGGAAGAAGA ACTAAATAAC
    TATATTTGCA TGGGCAAATC CGGTAGTCAT CTCCAGAGAA GTCAGCAGCA GAGGTACCAG
    CTAAGTCGAG GGGAAGAGCA CCCTGACTTT GATAAGGTTT TTAGAAAGAG GACTCACTCC
    TCAGGAACAT CACCTCCTAC AGTTTCACAC CAGAAGACAC CATCACAGTC CTCTATTGAG
    GAATATACAG AGATGATGCC TGCTCATCCT GTTCGTTTAA CATCATTTAG ACATTCTGCA
    TTTGTACCCA CCTACTCCTA TCCAGAAGAG TGCCTGGACC TCCATTTAGA GGGTAGCAGA
    GCTAACCACA CAGATGACGG ATACATGCCT ATGTCACCTG GTGTTGCTCC TGTGCCTACA
    AAATCAAATG ACTATATGCC TATGAGCCCT AAGAGTGTGT CGGCCCCTCA ACAGATCATT
    AACCCAAGAC GGCACTCTGC TGTAGATTCA AATGGTTATA TGATGATGTC ACCCAGTGGT
    AGCTGTTCTC CGGATAGCAC CAATTACAGC AAGATATGGA CCAATGGTAC CAATCCAAAG
    TTGTCCATAG ATAGCATTGA GGGCAAACTG CCTTGCAGTG ACTACATTAA TATGTCTCCT
    GCTAGTGGCT CTACTACAAG CACGCCTCCA GACTCTTATC TGAATTCTAT TGAAGAGTCG
    ACCAAGCCTG TATATTCCTA CTTTTCCCTG CCCAGGTCCT TCAAACATGT TCACAGGAAA
    AGTGAAGATG GCAATTTGCG CATCACTGCA AATTCTGGAC ATAATCTATA CACTGAGGAC
    TCTTCCTCCT CTTCCACAAG CAGTGATAGT CTAGGGGGGC AGGACCCTCA GCAGCCTAGA
    AAAGGGGAGA CCTGCATTCA AGGGAAGAGA TTGACCAGAC CCACCAGGCT TTCATTGGAA
    AACAGCAGTA AGGCCAGCAC ACTGCCAAGG GCGAGAGAAC CTGCTTTGCC CCCAGAGCCA
    AAAAGTCCTG GGGAGTATGT TAATATAGAG TTTAACGACA AGGTATTTTC AGGGGGCTTG
    ATGCCTTCCA TGTGTCCCCT TCCTTTTGTG CAGAGCAGAG TTGTGCCTCA AAGAGAGAAC
    TTGTCTGAAT ACATGAACAT GGATCTTGGA GTTTGGAGAG CTAAAACTTC CTATGCTTCC
    ACCTCATCTT CATCATATGA ACCACCATAC AAGCCGGTCA GCTCAGTGTG CCCTACTGAA
    ACTTGCAGTA GTAGTCGACC ACCTATTAGA GGAAAGACAA ACTCACGAGA CTATATGAGC
    ATGCAACTTA GTGCTCTGTG TTCAGATTAC AGCCAAGTTC CACCTACCAG AATAACTGCC
    AAACCCATTA CTCTTTCTTC TAATAAAAGT AATTATGCAG AGATGTCAAG TGGCGGAGTT
    TCTGATAATA TTCCTGCTAT TCCCCAAACT TCTAACTCAA GCCTATCTGA AGCATCTCGC
    TCTTCCCTTT TGGGCCAGGG CTCTGGCCCT AGCGCCTTTA CTCGGGTCAG TCTAAGCCCA
    AACCGCAATC AGAGTGCCAA AGTCATCAGG GCCGGGGACC CACAGGGTAG GAGGAGGCAC
    AGCTCAGAGA CATTCTCCTC TACTCCTACT ACAGCCAGAG TTACTTCTGG CCCAGTTTCT
    GGGGAGGATG TCAAGAGACA TAGTTCTGCC TCCTTTGAAA ATGTGTGGCT AAAACCAGGA
    GAGATTGCCA GGAGAGATTC CCTTCAGCCT TCTGATCACA CACATAATGG CCTGAATTAT
    ATTGACTTAG ATCTAGCTAA GGACTTGAGT GGCCTGGATC ATTGCAACTC CCACCAATCT
    GGAGTAAGCC ACCCATCAGA TGACCTGAGC ACATACGCCA GCATCACTTT CCACAAGCTA
    GAGGAGCACC GGAATCAGGC AGAAACAGAA GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937134.1
    CDS929..4204
    Translation

    Target ORF information:

    RefSeq Version XM_002937088.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis insulin receptor substrate 1 (irs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937088.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTAGCC CCACAGATCC ACAGGCTCAG GAGAACTTTT CTGATGTCAG AAAGGTGGGC 
    TATCTAAGGA AACCTAAGAG CATGCACAAG AGGTTTTTTG TGCTCAGGTC TGCCAGTGAG
    TCAGGTTTGG CCCGTTTGGA GTATTATGAA AATGAGAAAA AGTGGAGGCA TAAGTCTGGG
    GCCCCAAAGA GGTCAATCCC TCTTGAGAGC TGCTTTAATA TTAACAAGAG GGCTGATTCA
    AAAAACAAAC ATTTGGTTGC TTTGTACACA AAGGAGGAAT GCTTTGCAAT TGCTGCTGAA
    TGTGAACAAG AACAGGATGG TTGGTATCAG GCTCTAGTGG ATCTTCACAA CCGTGGTAAG
    ACCCACCACC AACACCATAA CCATGACGGT GCAACCAATG GTGTACATGA TGGTTTAAAT
    GGAGATGACG TTTATGGGGA GGTTACTCCT CCAGGACTGG CCTTCAAAGA GGTATGGCAG
    GTGATCATGA AGCCCAAGGG TTTAGGGCAA CTTAAGAATT TAGTTGGTAT TTACCGCCTG
    TGCCTCACCA ACCGAACAAT AAGTTTGGTA AAACTGAACT CTGATGCAGC CGCTGTGGTA
    TTGCAACTTA TGAATATAAG GAGATGTGGG CATTCAGAAA ATTTTTTCTT TATAGAAGTG
    GGTCGTTCAG CAGTCACCGG AGCTGGTGAA TTCTGGATGC AAGTGGATGA TTCAGTAGTT
    GCTCAAAACA TGCATGAAAC CATACTAGAA GCTATGAAAG CTCTGAGCGA TGAATTTCGG
    CCTCGGAGTA AGAGTCAGTC TTCATCTAAC TGCTCTAATC CTATATCTGT ACCTCTTAGG
    AGGCATCACC TTAATCATCC ACCACCCAGC CAGGTAGGAC TAAATAGAAG AGCTCGTACA
    GAGAGTGTTA CTGCCACATC ACCTGCAGGG GGTGTGGCAA AGCATGGATC ATCCTCCTCT
    TTCAGAGTCC GGGCATCTAG TGATGGAGAA GGAACAATGT CAAGGCCAGC CTCCATGGAA
    GGGAGCCCAG TAAGTCCCAG TGCTAGCAGA GCTCAGTCGC ACCGGCATCG GGGAAGTTCT
    CGACTTCACC CGCCTCTTAA CCATAGTCGG TCTATACCAA TGCCAGCAAC TCGCTGCTCT
    CCATCGGCTA CCAGCCCTGT CAGCTTGTCC TCCAGTAGTA CCAGTGGACA TGGTTCCACT
    TCTGATTGCA TGTGCCCTCG AAGATCCAGT GCCTCTGTTT CTGGTTCACC CAGTGATGGT
    GGTTTTATTT CTTCAGATGA ATATGGGTCT AGCCCTTGTG ACTTTAGAAG TTCTTTTCGC
    AGTGTAACCC CAGATTCTTT GGGCCACACT CCACCAGCTA GGGAAGAAGA ACTAAATAAC
    TATATTTGCA TGGGCAAATC CGGTAGTCAT CTCCAGAGAA GTCAGCAGCA GAGGTACCAG
    CTAAGTCGAG GGGAAGAGCA CCCTGACTTT GATAAGGTTT TTAGAAAGAG GACTCACTCC
    TCAGGAACAT CACCTCCTAC AGTTTCACAC CAGAAGACAC CATCACAGTC CTCTATTGAG
    GAATATACAG AGATGATGCC TGCTCATCCT GTTCGTTTAA CATCATTTAG ACATTCTGCA
    TTTGTACCCA CCTACTCCTA TCCAGAAGAG TGCCTGGACC TCCATTTAGA GGGTAGCAGA
    GCTAACCACA CAGATGACGG ATACATGCCT ATGTCACCTG GTGTTGCTCC TGTGCCTACA
    AAATCAAATG ACTATATGCC TATGAGCCCT AAGAGTGTGT CGGCCCCTCA ACAGATCATT
    AACCCAAGAC GGCACTCTGC TGTAGATTCA AATGGTTATA TGATGATGTC ACCCAGTGGT
    AGCTGTTCTC CGGATAGCAC CAATTACAGC AAGATATGGA CCAATGGTAC CAATCCAAAG
    TTGTCCATAG ATAGCATTGA GGGCAAACTG CCTTGCAGTG ACTACATTAA TATGTCTCCT
    GCTAGTGGCT CTACTACAAG CACGCCTCCA GACTCTTATC TGAATTCTAT TGAAGAGTCG
    ACCAAGCCTG TATATTCCTA CTTTTCCCTG CCCAGGTCCT TCAAACATGT TCACAGGAAA
    AGTGAAGATG GCAATTTGCG CATCACTGCA AATTCTGGAC ATAATCTATA CACTGAGGAC
    TCTTCCTCCT CTTCCACAAG CAGTGATAGT CTAGGGGGGC AGGACCCTCA GCAGCCTAGA
    AAAGGGGAGA CCTGCATTCA AGGGAAGAGA TTGACCAGAC CCACCAGGCT TTCATTGGAA
    AACAGCAGTA AGGCCAGCAC ACTGCCAAGG GCGAGAGAAC CTGCTTTGCC CCCAGAGCCA
    AAAAGTCCTG GGGAGTATGT TAATATAGAG TTTAACGACA AGGTATTTTC AGGGGGCTTG
    ATGCCTTCCA TGTGTCCCCT TCCTTTTGTG CAGAGCAGAG TTGTGCCTCA AAGAGAGAAC
    TTGTCTGAAT ACATGAACAT GGATCTTGGA GTTTGGAGAG CTAAAACTTC CTATGCTTCC
    ACCTCATCTT CATCATATGA ACCACCATAC AAGCCGGTCA GCTCAGTGTG CCCTACTGAA
    ACTTGCAGTA GTAGTCGACC ACCTATTAGA GGAAAGACAA ACTCACGAGA CTATATGAGC
    ATGCAACTTA GTGCTCTGTG TTCAGATTAC AGCCAAGTTC CACCTACCAG AATAACTGCC
    AAACCCATTA CTCTTTCTTC TAATAAAAGT AATTATGCAG AGATGTCAAG TGGCGGAGTT
    TCTGATAATA TTCCTGCTAT TCCCCAAACT TCTAACTCAA GCCTATCTGA AGCATCTCGC
    TCTTCCCTTT TGGGCCAGGG CTCTGGCCCT AGCGCCTTTA CTCGGGTCAG TCTAAGCCCA
    AACCGCAATC AGAGTGCCAA AGTCATCAGG GCCGGGGACC CACAGGGTAG GAGGAGGCAC
    AGCTCAGAGA CATTCTCCTC TACTCCTACT ACAGCCAGAG TTACTTCTGG CCCAGTTTCT
    GGGGAGGATG TCAAGAGACA TAGTTCTGCC TCCTTTGAAA ATGTGTGGCT AAAACCAGGA
    GAGATTGCCA GGAGAGATTC CCTTCAGCCT TCTGATCACA CACATAATGG CCTGAATTAT
    ATTGACTTAG ATCTAGCTAA GGACTTGAGT GGCCTGGATC ATTGCAACTC CCACCAATCT
    GGAGTAAGCC ACCCATCAGA TGACCTGAGC ACATACGCCA GCATCACTTT CCACAAGCTA
    GAGGAGCACC GGAATCAGGC AGAAACAGAA GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10306
    Clone ID Related Accession (Same CDS sequence) XM_012963449.2 , XM_012963449.3
    Accession Version XM_012963449.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product insulin receptor substrate 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTAGCC CCACAGATCC ACAGGCTCAG GAGAACTTTT CTGATGTCAG AAAGGTGGGC 
    TATCTAAGGA AACCTAAGAG CATGCACAAG AGGTTTTTTG TGCTCAGGTC TGCCAGTGAG
    TCAGGTTTGG CCCGTTTGGA GTATTATGAA AATGAGAAAA AGTGGAGGCA TAAGTCTGGG
    GCCCCAAAGA GGTCAATCCC TCTTGAGAGC TGCTTTAATA TTAACAAGAG GGCTGATTCA
    AAAAACAAAC ATTTGGTTGC TTTGTACACA AAGGAGGAAT GCTTTGCAAT TGCTGCTGAA
    TGTGAACAAG AACAGGATGG TTGGTATCAG GCTCTAGTGG ATCTTCACAA CCGTGGTAAG
    ACCCACCACC AACACCATAA CCATGACGGT GCAACCAATG GTGTACATGA TGGTTTAAAT
    GGAGATGACG TTTATGGGGA GGTTACTCCT CCAGGACTGG CCTTCAAAGA GGTATGGCAG
    GTGATCATGA AGCCCAAGGG TTTAGGGCAA CTTAAGAATT TAGTTGGTAT TTACCGCCTG
    TGCCTCACCA ACCGAACAAT AAGTTTGGTA AAACTGAACT CTGATGCAGC CGCTGTGGTA
    TTGCAACTTA TGAATATAAG GAGATGTGGG CATTCAGAAA ATTTTTTCTT TATAGAAGTG
    GGTCGTTCAG CAGTCACCGG AGCTGGTGAA TTCTGGATGC AAGTGGATGA TTCAGTAGTT
    GCTCAAAACA TGCATGAAAC CATACTAGAA GCTATGAAAG CTCTGAGCGA TGAATTTCGG
    CCTCGGAGTA AGAGTCAGTC TTCATCTAAC TGCTCTAATC CTATATCTGT ACCTCTTAGG
    AGGCATCACC TTAATCATCC ACCACCCAGC CAGGTAGGAC TAAATAGAAG AGCTCGTACA
    GAGAGTGTTA CTGCCACATC ACCTGCAGGG GGTGTGGCAA AGCATGGATC ATCCTCCTCT
    TTCAGAGTCC GGGCATCTAG TGATGGAGAA GGAACAATGT CAAGGCCAGC CTCCATGGAA
    GGGAGCCCAG TAAGTCCCAG TGCTAGCAGA GCTCAGTCGC ACCGGCATCG GGGAAGTTCT
    CGACTTCACC CGCCTCTTAA CCATAGTCGG TCTATACCAA TGCCAGCAAC TCGCTGCTCT
    CCATCGGCTA CCAGCCCTGT CAGCTTGTCC TCCAGTAGTA CCAGTGGACA TGGTTCCACT
    TCTGATTGCA TGTGCCCTCG AAGATCCAGT GCCTCTGTTT CTGGTTCACC CAGTGATGGT
    GGTTTTATTT CTTCAGATGA ATATGGGTCT AGCCCTTGTG ACTTTAGAAG TTCTTTTCGC
    AGTGTAACCC CAGATTCTTT GGGCCACACT CCACCAGCTA GGGAAGAAGA ACTAAATAAC
    TATATTTGCA TGGGCAAATC CGGTAGTCAT CTCCAGAGAA GTCAGCAGCA GAGGTACCAG
    CTAAGTCGAG GGGAAGAGCA CCCTGACTTT GATAAGGTTT TTAGAAAGAG GACTCACTCC
    TCAGGAACAT CACCTCCTAC AGTTTCACAC CAGAAGACAC CATCACAGTC CTCTATTGAG
    GAATATACAG AGATGATGCC TGCTCATCCT GTTCGTTTAA CATCATTTAG ACATTCTGCA
    TTTGTACCCA CCTACTCCTA TCCAGAAGAG TGCCTGGACC TCCATTTAGA GGGTAGCAGA
    GCTAACCACA CAGATGACGG ATACATGCCT ATGTCACCTG GTGTTGCTCC TGTGCCTACA
    AAATCAAATG ACTATATGCC TATGAGCCCT AAGAGTGTGT CGGCCCCTCA ACAGATCATT
    AACCCAAGAC GGCACTCTGC TGTAGATTCA AATGGTTATA TGATGATGTC ACCCAGTGGT
    AGCTGTTCTC CGGATAGCAC CAATTACAGC AAGATATGGA CCAATGGTAC CAATCCAAAG
    TTGTCCATAG ATAGCATTGA GGGCAAACTG CCTTGCAGTG ACTACATTAA TATGTCTCCT
    GCTAGTGGCT CTACTACAAG CACGCCTCCA GACTCTTATC TGAATTCTAT TGAAGAGTCG
    ACCAAGCCTG TATATTCCTA CTTTTCCCTG CCCAGGTCCT TCAAACATGT TCACAGGAAA
    AGTGAAGATG GCAATTTGCG CATCACTGCA AATTCTGGAC ATAATCTATA CACTGAGGAC
    TCTTCCTCCT CTTCCACAAG CAGTGATAGT CTAGGGGGGC AGGACCCTCA GCAGCCTAGA
    AAAGGGGAGA CCTGCATTCA AGGGAAGAGA TTGACCAGAC CCACCAGGCT TTCATTGGAA
    AACAGCAGTA AGGCCAGCAC ACTGCCAAGG GCGAGAGAAC CTGCTTTGCC CCCAGAGCCA
    AAAAGTCCTG GGGAGTATGT TAATATAGAG TTTAACGACA AGGTATTTTC AGGGGGCTTG
    ATGCCTTCCA TGTGTCCCCT TCCTTTTGTG CAGAGCAGAG TTGTGCCTCA AAGAGAGAAC
    TTGTCTGAAT ACATGAACAT GGATCTTGGA GTTTGGAGAG CTAAAACTTC CTATGCTTCC
    ACCTCATCTT CATCATATGA ACCACCATAC AAGCCGGTCA GCTCAGTGTG CCCTACTGAA
    ACTTGCAGTA GTAGTCGACC ACCTATTAGA GGAAAGACAA ACTCACGAGA CTATATGAGC
    ATGCAACTTA GTGCTCTGTG TTCAGATTAC AGCCAAGTTC CACCTACCAG AATAACTGCC
    AAACCCATTA CTCTTTCTTC TAATAAAAGT AATTATGCAG AGATGTCAAG TGGCGGAGTT
    TCTGATAATA TTCCTGCTAT TCCCCAAACT TCTAACTCAA GCCTATCTGA AGCATCTCGC
    TCTTCCCTTT TGGGCCAGGG CTCTGGCCCT AGCGCCTTTA CTCGGGTCAG TCTAAGCCCA
    AACCGCAATC AGAGTGCCAA AGTCATCAGG GCCGGGGACC CACAGGGTAG GAGGAGGCAC
    AGCTCAGAGA CATTCTCCTC TACTCCTACT ACAGCCAGAG TTACTTCTGG CCCAGTTTCT
    GGGGAGGATG TCAAGAGACA TAGTTCTGCC TCCTTTGAAA ATGTGTGGCT AAAACCAGGA
    GAGATTGCCA GGAGAGATTC CCTTCAGCCT TCTGATCACA CACATAATGG CCTGAATTAT
    ATTGACTTAG ATCTAGCTAA GGACTTGAGT GGCCTGGATC ATTGCAACTC CCACCAATCT
    GGAGTAAGCC ACCCATCAGA TGACCTGAGC ACATACGCCA GCATCACTTT CCACAAGCTA
    GAGGAGCACC GGAATCAGGC AGAAACAGAA GATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818903.1
    CDS930..4205
    Translation

    Target ORF information:

    RefSeq Version XM_012963449.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis insulin receptor substrate 1 (irs1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963449.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTAGCC CCACAGATCC ACAGGCTCAG GAGAACTTTT CTGATGTCAG AAAGGTGGGC 
    TATCTAAGGA AACCTAAGAG CATGCACAAG AGGTTTTTTG TGCTCAGGTC TGCCAGTGAG
    TCAGGTTTGG CCCGTTTGGA GTATTATGAA AATGAGAAAA AGTGGAGGCA TAAGTCTGGG
    GCCCCAAAGA GGTCAATCCC TCTTGAGAGC TGCTTTAATA TTAACAAGAG GGCTGATTCA
    AAAAACAAAC ATTTGGTTGC TTTGTACACA AAGGAGGAAT GCTTTGCAAT TGCTGCTGAA
    TGTGAACAAG AACAGGATGG TTGGTATCAG GCTCTAGTGG ATCTTCACAA CCGTGGTAAG
    ACCCACCACC AACACCATAA CCATGACGGT GCAACCAATG GTGTACATGA TGGTTTAAAT
    GGAGATGACG TTTATGGGGA GGTTACTCCT CCAGGACTGG CCTTCAAAGA GGTATGGCAG
    GTGATCATGA AGCCCAAGGG TTTAGGGCAA CTTAAGAATT TAGTTGGTAT TTACCGCCTG
    TGCCTCACCA ACCGAACAAT AAGTTTGGTA AAACTGAACT CTGATGCAGC CGCTGTGGTA
    TTGCAACTTA TGAATATAAG GAGATGTGGG CATTCAGAAA ATTTTTTCTT TATAGAAGTG
    GGTCGTTCAG CAGTCACCGG AGCTGGTGAA TTCTGGATGC AAGTGGATGA TTCAGTAGTT
    GCTCAAAACA TGCATGAAAC CATACTAGAA GCTATGAAAG CTCTGAGCGA TGAATTTCGG
    CCTCGGAGTA AGAGTCAGTC TTCATCTAAC TGCTCTAATC CTATATCTGT ACCTCTTAGG
    AGGCATCACC TTAATCATCC ACCACCCAGC CAGGTAGGAC TAAATAGAAG AGCTCGTACA
    GAGAGTGTTA CTGCCACATC ACCTGCAGGG GGTGTGGCAA AGCATGGATC ATCCTCCTCT
    TTCAGAGTCC GGGCATCTAG TGATGGAGAA GGAACAATGT CAAGGCCAGC CTCCATGGAA
    GGGAGCCCAG TAAGTCCCAG TGCTAGCAGA GCTCAGTCGC ACCGGCATCG GGGAAGTTCT
    CGACTTCACC CGCCTCTTAA CCATAGTCGG TCTATACCAA TGCCAGCAAC TCGCTGCTCT
    CCATCGGCTA CCAGCCCTGT CAGCTTGTCC TCCAGTAGTA CCAGTGGACA TGGTTCCACT
    TCTGATTGCA TGTGCCCTCG AAGATCCAGT GCCTCTGTTT CTGGTTCACC CAGTGATGGT
    GGTTTTATTT CTTCAGATGA ATATGGGTCT AGCCCTTGTG ACTTTAGAAG TTCTTTTCGC
    AGTGTAACCC CAGATTCTTT GGGCCACACT CCACCAGCTA GGGAAGAAGA ACTAAATAAC
    TATATTTGCA TGGGCAAATC CGGTAGTCAT CTCCAGAGAA GTCAGCAGCA GAGGTACCAG
    CTAAGTCGAG GGGAAGAGCA CCCTGACTTT GATAAGGTTT TTAGAAAGAG GACTCACTCC
    TCAGGAACAT CACCTCCTAC AGTTTCACAC CAGAAGACAC CATCACAGTC CTCTATTGAG
    GAATATACAG AGATGATGCC TGCTCATCCT GTTCGTTTAA CATCATTTAG ACATTCTGCA
    TTTGTACCCA CCTACTCCTA TCCAGAAGAG TGCCTGGACC TCCATTTAGA GGGTAGCAGA
    GCTAACCACA CAGATGACGG ATACATGCCT ATGTCACCTG GTGTTGCTCC TGTGCCTACA
    AAATCAAATG ACTATATGCC TATGAGCCCT AAGAGTGTGT CGGCCCCTCA ACAGATCATT
    AACCCAAGAC GGCACTCTGC TGTAGATTCA AATGGTTATA TGATGATGTC ACCCAGTGGT
    AGCTGTTCTC CGGATAGCAC CAATTACAGC AAGATATGGA CCAATGGTAC CAATCCAAAG
    TTGTCCATAG ATAGCATTGA GGGCAAACTG CCTTGCAGTG ACTACATTAA TATGTCTCCT
    GCTAGTGGCT CTACTACAAG CACGCCTCCA GACTCTTATC TGAATTCTAT TGAAGAGTCG
    ACCAAGCCTG TATATTCCTA CTTTTCCCTG CCCAGGTCCT TCAAACATGT TCACAGGAAA
    AGTGAAGATG GCAATTTGCG CATCACTGCA AATTCTGGAC ATAATCTATA CACTGAGGAC
    TCTTCCTCCT CTTCCACAAG CAGTGATAGT CTAGGGGGGC AGGACCCTCA GCAGCCTAGA
    AAAGGGGAGA CCTGCATTCA AGGGAAGAGA TTGACCAGAC CCACCAGGCT TTCATTGGAA
    AACAGCAGTA AGGCCAGCAC ACTGCCAAGG GCGAGAGAAC CTGCTTTGCC CCCAGAGCCA
    AAAAGTCCTG GGGAGTATGT TAATATAGAG TTTAACGACA AGGTATTTTC AGGGGGCTTG
    ATGCCTTCCA TGTGTCCCCT TCCTTTTGTG CAGAGCAGAG TTGTGCCTCA AAGAGAGAAC
    TTGTCTGAAT ACATGAACAT GGATCTTGGA GTTTGGAGAG CTAAAACTTC CTATGCTTCC
    ACCTCATCTT CATCATATGA ACCACCATAC AAGCCGGTCA GCTCAGTGTG CCCTACTGAA
    ACTTGCAGTA GTAGTCGACC ACCTATTAGA GGAAAGACAA ACTCACGAGA CTATATGAGC
    ATGCAACTTA GTGCTCTGTG TTCAGATTAC AGCCAAGTTC CACCTACCAG AATAACTGCC
    AAACCCATTA CTCTTTCTTC TAATAAAAGT AATTATGCAG AGATGTCAAG TGGCGGAGTT
    TCTGATAATA TTCCTGCTAT TCCCCAAACT TCTAACTCAA GCCTATCTGA AGCATCTCGC
    TCTTCCCTTT TGGGCCAGGG CTCTGGCCCT AGCGCCTTTA CTCGGGTCAG TCTAAGCCCA
    AACCGCAATC AGAGTGCCAA AGTCATCAGG GCCGGGGACC CACAGGGTAG GAGGAGGCAC
    AGCTCAGAGA CATTCTCCTC TACTCCTACT ACAGCCAGAG TTACTTCTGG CCCAGTTTCT
    GGGGAGGATG TCAAGAGACA TAGTTCTGCC TCCTTTGAAA ATGTGTGGCT AAAACCAGGA
    GAGATTGCCA GGAGAGATTC CCTTCAGCCT TCTGATCACA CACATAATGG CCTGAATTAT
    ATTGACTTAG ATCTAGCTAA GGACTTGAGT GGCCTGGATC ATTGCAACTC CCACCAATCT
    GGAGTAAGCC ACCCATCAGA TGACCTGAGC ACATACGCCA GCATCACTTT CCACAAGCTA
    GAGGAGCACC GGAATCAGGC AGAAACAGAA GATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10305
    Clone ID Related Accession (Same CDS sequence) XM_002937088.3 , XM_002937088.4
    Accession Version XM_002937088.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product insulin receptor substrate 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002937088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTAGCC CCACAGATCC ACAGGCTCAG GAGAACTTTT CTGATGTCAG AAAGGTGGGC 
    TATCTAAGGA AACCTAAGAG CATGCACAAG AGGTTTTTTG TGCTCAGGTC TGCCAGTGAG
    TCAGGTTTGG CCCGTTTGGA GTATTATGAA AATGAGAAAA AGTGGAGGCA TAAGTCTGGG
    GCCCCAAAGA GGTCAATCCC TCTTGAGAGC TGCTTTAATA TTAACAAGAG GGCTGATTCA
    AAAAACAAAC ATTTGGTTGC TTTGTACACA AAGGAGGAAT GCTTTGCAAT TGCTGCTGAA
    TGTGAACAAG AACAGGATGG TTGGTATCAG GCTCTAGTGG ATCTTCACAA CCGTGGTAAG
    ACCCACCACC AACACCATAA CCATGACGGT GCAACCAATG GTGTACATGA TGGTTTAAAT
    GGAGATGACG TTTATGGGGA GGTTACTCCT CCAGGACTGG CCTTCAAAGA GGTATGGCAG
    GTGATCATGA AGCCCAAGGG TTTAGGGCAA CTTAAGAATT TAGTTGGTAT TTACCGCCTG
    TGCCTCACCA ACCGAACAAT AAGTTTGGTA AAACTGAACT CTGATGCAGC CGCTGTGGTA
    TTGCAACTTA TGAATATAAG GAGATGTGGG CATTCAGAAA ATTTTTTCTT TATAGAAGTG
    GGTCGTTCAG CAGTCACCGG AGCTGGTGAA TTCTGGATGC AAGTGGATGA TTCAGTAGTT
    GCTCAAAACA TGCATGAAAC CATACTAGAA GCTATGAAAG CTCTGAGCGA TGAATTTCGG
    CCTCGGAGTA AGAGTCAGTC TTCATCTAAC TGCTCTAATC CTATATCTGT ACCTCTTAGG
    AGGCATCACC TTAATCATCC ACCACCCAGC CAGGTAGGAC TAAATAGAAG AGCTCGTACA
    GAGAGTGTTA CTGCCACATC ACCTGCAGGG GGTGTGGCAA AGCATGGATC ATCCTCCTCT
    TTCAGAGTCC GGGCATCTAG TGATGGAGAA GGAACAATGT CAAGGCCAGC CTCCATGGAA
    GGGAGCCCAG TAAGTCCCAG TGCTAGCAGA GCTCAGTCGC ACCGGCATCG GGGAAGTTCT
    CGACTTCACC CGCCTCTTAA CCATAGTCGG TCTATACCAA TGCCAGCAAC TCGCTGCTCT
    CCATCGGCTA CCAGCCCTGT CAGCTTGTCC TCCAGTAGTA CCAGTGGACA TGGTTCCACT
    TCTGATTGCA TGTGCCCTCG AAGATCCAGT GCCTCTGTTT CTGGTTCACC CAGTGATGGT
    GGTTTTATTT CTTCAGATGA ATATGGGTCT AGCCCTTGTG ACTTTAGAAG TTCTTTTCGC
    AGTGTAACCC CAGATTCTTT GGGCCACACT CCACCAGCTA GGGAAGAAGA ACTAAATAAC
    TATATTTGCA TGGGCAAATC CGGTAGTCAT CTCCAGAGAA GTCAGCAGCA GAGGTACCAG
    CTAAGTCGAG GGGAAGAGCA CCCTGACTTT GATAAGGTTT TTAGAAAGAG GACTCACTCC
    TCAGGAACAT CACCTCCTAC AGTTTCACAC CAGAAGACAC CATCACAGTC CTCTATTGAG
    GAATATACAG AGATGATGCC TGCTCATCCT GTTCGTTTAA CATCATTTAG ACATTCTGCA
    TTTGTACCCA CCTACTCCTA TCCAGAAGAG TGCCTGGACC TCCATTTAGA GGGTAGCAGA
    GCTAACCACA CAGATGACGG ATACATGCCT ATGTCACCTG GTGTTGCTCC TGTGCCTACA
    AAATCAAATG ACTATATGCC TATGAGCCCT AAGAGTGTGT CGGCCCCTCA ACAGATCATT
    AACCCAAGAC GGCACTCTGC TGTAGATTCA AATGGTTATA TGATGATGTC ACCCAGTGGT
    AGCTGTTCTC CGGATAGCAC CAATTACAGC AAGATATGGA CCAATGGTAC CAATCCAAAG
    TTGTCCATAG ATAGCATTGA GGGCAAACTG CCTTGCAGTG ACTACATTAA TATGTCTCCT
    GCTAGTGGCT CTACTACAAG CACGCCTCCA GACTCTTATC TGAATTCTAT TGAAGAGTCG
    ACCAAGCCTG TATATTCCTA CTTTTCCCTG CCCAGGTCCT TCAAACATGT TCACAGGAAA
    AGTGAAGATG GCAATTTGCG CATCACTGCA AATTCTGGAC ATAATCTATA CACTGAGGAC
    TCTTCCTCCT CTTCCACAAG CAGTGATAGT CTAGGGGGGC AGGACCCTCA GCAGCCTAGA
    AAAGGGGAGA CCTGCATTCA AGGGAAGAGA TTGACCAGAC CCACCAGGCT TTCATTGGAA
    AACAGCAGTA AGGCCAGCAC ACTGCCAAGG GCGAGAGAAC CTGCTTTGCC CCCAGAGCCA
    AAAAGTCCTG GGGAGTATGT TAATATAGAG TTTAACGACA AGGTATTTTC AGGGGGCTTG
    ATGCCTTCCA TGTGTCCCCT TCCTTTTGTG CAGAGCAGAG TTGTGCCTCA AAGAGAGAAC
    TTGTCTGAAT ACATGAACAT GGATCTTGGA GTTTGGAGAG CTAAAACTTC CTATGCTTCC
    ACCTCATCTT CATCATATGA ACCACCATAC AAGCCGGTCA GCTCAGTGTG CCCTACTGAA
    ACTTGCAGTA GTAGTCGACC ACCTATTAGA GGAAAGACAA ACTCACGAGA CTATATGAGC
    ATGCAACTTA GTGCTCTGTG TTCAGATTAC AGCCAAGTTC CACCTACCAG AATAACTGCC
    AAACCCATTA CTCTTTCTTC TAATAAAAGT AATTATGCAG AGATGTCAAG TGGCGGAGTT
    TCTGATAATA TTCCTGCTAT TCCCCAAACT TCTAACTCAA GCCTATCTGA AGCATCTCGC
    TCTTCCCTTT TGGGCCAGGG CTCTGGCCCT AGCGCCTTTA CTCGGGTCAG TCTAAGCCCA
    AACCGCAATC AGAGTGCCAA AGTCATCAGG GCCGGGGACC CACAGGGTAG GAGGAGGCAC
    AGCTCAGAGA CATTCTCCTC TACTCCTACT ACAGCCAGAG TTACTTCTGG CCCAGTTTCT
    GGGGAGGATG TCAAGAGACA TAGTTCTGCC TCCTTTGAAA ATGTGTGGCT AAAACCAGGA
    GAGATTGCCA GGAGAGATTC CCTTCAGCCT TCTGATCACA CACATAATGG CCTGAATTAT
    ATTGACTTAG ATCTAGCTAA GGACTTGAGT GGCCTGGATC ATTGCAACTC CCACCAATCT
    GGAGTAAGCC ACCCATCAGA TGACCTGAGC ACATACGCCA GCATCACTTT CCACAAGCTA
    GAGGAGCACC GGAATCAGGC AGAAACAGAA GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937134.1
    CDS952..4227
    Translation

    Target ORF information:

    RefSeq Version XM_002937088.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis insulin receptor substrate 1 (irs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937088.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTAGCC CCACAGATCC ACAGGCTCAG GAGAACTTTT CTGATGTCAG AAAGGTGGGC 
    TATCTAAGGA AACCTAAGAG CATGCACAAG AGGTTTTTTG TGCTCAGGTC TGCCAGTGAG
    TCAGGTTTGG CCCGTTTGGA GTATTATGAA AATGAGAAAA AGTGGAGGCA TAAGTCTGGG
    GCCCCAAAGA GGTCAATCCC TCTTGAGAGC TGCTTTAATA TTAACAAGAG GGCTGATTCA
    AAAAACAAAC ATTTGGTTGC TTTGTACACA AAGGAGGAAT GCTTTGCAAT TGCTGCTGAA
    TGTGAACAAG AACAGGATGG TTGGTATCAG GCTCTAGTGG ATCTTCACAA CCGTGGTAAG
    ACCCACCACC AACACCATAA CCATGACGGT GCAACCAATG GTGTACATGA TGGTTTAAAT
    GGAGATGACG TTTATGGGGA GGTTACTCCT CCAGGACTGG CCTTCAAAGA GGTATGGCAG
    GTGATCATGA AGCCCAAGGG TTTAGGGCAA CTTAAGAATT TAGTTGGTAT TTACCGCCTG
    TGCCTCACCA ACCGAACAAT AAGTTTGGTA AAACTGAACT CTGATGCAGC CGCTGTGGTA
    TTGCAACTTA TGAATATAAG GAGATGTGGG CATTCAGAAA ATTTTTTCTT TATAGAAGTG
    GGTCGTTCAG CAGTCACCGG AGCTGGTGAA TTCTGGATGC AAGTGGATGA TTCAGTAGTT
    GCTCAAAACA TGCATGAAAC CATACTAGAA GCTATGAAAG CTCTGAGCGA TGAATTTCGG
    CCTCGGAGTA AGAGTCAGTC TTCATCTAAC TGCTCTAATC CTATATCTGT ACCTCTTAGG
    AGGCATCACC TTAATCATCC ACCACCCAGC CAGGTAGGAC TAAATAGAAG AGCTCGTACA
    GAGAGTGTTA CTGCCACATC ACCTGCAGGG GGTGTGGCAA AGCATGGATC ATCCTCCTCT
    TTCAGAGTCC GGGCATCTAG TGATGGAGAA GGAACAATGT CAAGGCCAGC CTCCATGGAA
    GGGAGCCCAG TAAGTCCCAG TGCTAGCAGA GCTCAGTCGC ACCGGCATCG GGGAAGTTCT
    CGACTTCACC CGCCTCTTAA CCATAGTCGG TCTATACCAA TGCCAGCAAC TCGCTGCTCT
    CCATCGGCTA CCAGCCCTGT CAGCTTGTCC TCCAGTAGTA CCAGTGGACA TGGTTCCACT
    TCTGATTGCA TGTGCCCTCG AAGATCCAGT GCCTCTGTTT CTGGTTCACC CAGTGATGGT
    GGTTTTATTT CTTCAGATGA ATATGGGTCT AGCCCTTGTG ACTTTAGAAG TTCTTTTCGC
    AGTGTAACCC CAGATTCTTT GGGCCACACT CCACCAGCTA GGGAAGAAGA ACTAAATAAC
    TATATTTGCA TGGGCAAATC CGGTAGTCAT CTCCAGAGAA GTCAGCAGCA GAGGTACCAG
    CTAAGTCGAG GGGAAGAGCA CCCTGACTTT GATAAGGTTT TTAGAAAGAG GACTCACTCC
    TCAGGAACAT CACCTCCTAC AGTTTCACAC CAGAAGACAC CATCACAGTC CTCTATTGAG
    GAATATACAG AGATGATGCC TGCTCATCCT GTTCGTTTAA CATCATTTAG ACATTCTGCA
    TTTGTACCCA CCTACTCCTA TCCAGAAGAG TGCCTGGACC TCCATTTAGA GGGTAGCAGA
    GCTAACCACA CAGATGACGG ATACATGCCT ATGTCACCTG GTGTTGCTCC TGTGCCTACA
    AAATCAAATG ACTATATGCC TATGAGCCCT AAGAGTGTGT CGGCCCCTCA ACAGATCATT
    AACCCAAGAC GGCACTCTGC TGTAGATTCA AATGGTTATA TGATGATGTC ACCCAGTGGT
    AGCTGTTCTC CGGATAGCAC CAATTACAGC AAGATATGGA CCAATGGTAC CAATCCAAAG
    TTGTCCATAG ATAGCATTGA GGGCAAACTG CCTTGCAGTG ACTACATTAA TATGTCTCCT
    GCTAGTGGCT CTACTACAAG CACGCCTCCA GACTCTTATC TGAATTCTAT TGAAGAGTCG
    ACCAAGCCTG TATATTCCTA CTTTTCCCTG CCCAGGTCCT TCAAACATGT TCACAGGAAA
    AGTGAAGATG GCAATTTGCG CATCACTGCA AATTCTGGAC ATAATCTATA CACTGAGGAC
    TCTTCCTCCT CTTCCACAAG CAGTGATAGT CTAGGGGGGC AGGACCCTCA GCAGCCTAGA
    AAAGGGGAGA CCTGCATTCA AGGGAAGAGA TTGACCAGAC CCACCAGGCT TTCATTGGAA
    AACAGCAGTA AGGCCAGCAC ACTGCCAAGG GCGAGAGAAC CTGCTTTGCC CCCAGAGCCA
    AAAAGTCCTG GGGAGTATGT TAATATAGAG TTTAACGACA AGGTATTTTC AGGGGGCTTG
    ATGCCTTCCA TGTGTCCCCT TCCTTTTGTG CAGAGCAGAG TTGTGCCTCA AAGAGAGAAC
    TTGTCTGAAT ACATGAACAT GGATCTTGGA GTTTGGAGAG CTAAAACTTC CTATGCTTCC
    ACCTCATCTT CATCATATGA ACCACCATAC AAGCCGGTCA GCTCAGTGTG CCCTACTGAA
    ACTTGCAGTA GTAGTCGACC ACCTATTAGA GGAAAGACAA ACTCACGAGA CTATATGAGC
    ATGCAACTTA GTGCTCTGTG TTCAGATTAC AGCCAAGTTC CACCTACCAG AATAACTGCC
    AAACCCATTA CTCTTTCTTC TAATAAAAGT AATTATGCAG AGATGTCAAG TGGCGGAGTT
    TCTGATAATA TTCCTGCTAT TCCCCAAACT TCTAACTCAA GCCTATCTGA AGCATCTCGC
    TCTTCCCTTT TGGGCCAGGG CTCTGGCCCT AGCGCCTTTA CTCGGGTCAG TCTAAGCCCA
    AACCGCAATC AGAGTGCCAA AGTCATCAGG GCCGGGGACC CACAGGGTAG GAGGAGGCAC
    AGCTCAGAGA CATTCTCCTC TACTCCTACT ACAGCCAGAG TTACTTCTGG CCCAGTTTCT
    GGGGAGGATG TCAAGAGACA TAGTTCTGCC TCCTTTGAAA ATGTGTGGCT AAAACCAGGA
    GAGATTGCCA GGAGAGATTC CCTTCAGCCT TCTGATCACA CACATAATGG CCTGAATTAT
    ATTGACTTAG ATCTAGCTAA GGACTTGAGT GGCCTGGATC ATTGCAACTC CCACCAATCT
    GGAGTAAGCC ACCCATCAGA TGACCTGAGC ACATACGCCA GCATCACTTT CCACAAGCTA
    GAGGAGCACC GGAATCAGGC AGAAACAGAA GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10306
    Clone ID Related Accession (Same CDS sequence) XM_012963449.2 , XM_012963449.3
    Accession Version XM_012963449.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product insulin receptor substrate 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTAGCC CCACAGATCC ACAGGCTCAG GAGAACTTTT CTGATGTCAG AAAGGTGGGC 
    TATCTAAGGA AACCTAAGAG CATGCACAAG AGGTTTTTTG TGCTCAGGTC TGCCAGTGAG
    TCAGGTTTGG CCCGTTTGGA GTATTATGAA AATGAGAAAA AGTGGAGGCA TAAGTCTGGG
    GCCCCAAAGA GGTCAATCCC TCTTGAGAGC TGCTTTAATA TTAACAAGAG GGCTGATTCA
    AAAAACAAAC ATTTGGTTGC TTTGTACACA AAGGAGGAAT GCTTTGCAAT TGCTGCTGAA
    TGTGAACAAG AACAGGATGG TTGGTATCAG GCTCTAGTGG ATCTTCACAA CCGTGGTAAG
    ACCCACCACC AACACCATAA CCATGACGGT GCAACCAATG GTGTACATGA TGGTTTAAAT
    GGAGATGACG TTTATGGGGA GGTTACTCCT CCAGGACTGG CCTTCAAAGA GGTATGGCAG
    GTGATCATGA AGCCCAAGGG TTTAGGGCAA CTTAAGAATT TAGTTGGTAT TTACCGCCTG
    TGCCTCACCA ACCGAACAAT AAGTTTGGTA AAACTGAACT CTGATGCAGC CGCTGTGGTA
    TTGCAACTTA TGAATATAAG GAGATGTGGG CATTCAGAAA ATTTTTTCTT TATAGAAGTG
    GGTCGTTCAG CAGTCACCGG AGCTGGTGAA TTCTGGATGC AAGTGGATGA TTCAGTAGTT
    GCTCAAAACA TGCATGAAAC CATACTAGAA GCTATGAAAG CTCTGAGCGA TGAATTTCGG
    CCTCGGAGTA AGAGTCAGTC TTCATCTAAC TGCTCTAATC CTATATCTGT ACCTCTTAGG
    AGGCATCACC TTAATCATCC ACCACCCAGC CAGGTAGGAC TAAATAGAAG AGCTCGTACA
    GAGAGTGTTA CTGCCACATC ACCTGCAGGG GGTGTGGCAA AGCATGGATC ATCCTCCTCT
    TTCAGAGTCC GGGCATCTAG TGATGGAGAA GGAACAATGT CAAGGCCAGC CTCCATGGAA
    GGGAGCCCAG TAAGTCCCAG TGCTAGCAGA GCTCAGTCGC ACCGGCATCG GGGAAGTTCT
    CGACTTCACC CGCCTCTTAA CCATAGTCGG TCTATACCAA TGCCAGCAAC TCGCTGCTCT
    CCATCGGCTA CCAGCCCTGT CAGCTTGTCC TCCAGTAGTA CCAGTGGACA TGGTTCCACT
    TCTGATTGCA TGTGCCCTCG AAGATCCAGT GCCTCTGTTT CTGGTTCACC CAGTGATGGT
    GGTTTTATTT CTTCAGATGA ATATGGGTCT AGCCCTTGTG ACTTTAGAAG TTCTTTTCGC
    AGTGTAACCC CAGATTCTTT GGGCCACACT CCACCAGCTA GGGAAGAAGA ACTAAATAAC
    TATATTTGCA TGGGCAAATC CGGTAGTCAT CTCCAGAGAA GTCAGCAGCA GAGGTACCAG
    CTAAGTCGAG GGGAAGAGCA CCCTGACTTT GATAAGGTTT TTAGAAAGAG GACTCACTCC
    TCAGGAACAT CACCTCCTAC AGTTTCACAC CAGAAGACAC CATCACAGTC CTCTATTGAG
    GAATATACAG AGATGATGCC TGCTCATCCT GTTCGTTTAA CATCATTTAG ACATTCTGCA
    TTTGTACCCA CCTACTCCTA TCCAGAAGAG TGCCTGGACC TCCATTTAGA GGGTAGCAGA
    GCTAACCACA CAGATGACGG ATACATGCCT ATGTCACCTG GTGTTGCTCC TGTGCCTACA
    AAATCAAATG ACTATATGCC TATGAGCCCT AAGAGTGTGT CGGCCCCTCA ACAGATCATT
    AACCCAAGAC GGCACTCTGC TGTAGATTCA AATGGTTATA TGATGATGTC ACCCAGTGGT
    AGCTGTTCTC CGGATAGCAC CAATTACAGC AAGATATGGA CCAATGGTAC CAATCCAAAG
    TTGTCCATAG ATAGCATTGA GGGCAAACTG CCTTGCAGTG ACTACATTAA TATGTCTCCT
    GCTAGTGGCT CTACTACAAG CACGCCTCCA GACTCTTATC TGAATTCTAT TGAAGAGTCG
    ACCAAGCCTG TATATTCCTA CTTTTCCCTG CCCAGGTCCT TCAAACATGT TCACAGGAAA
    AGTGAAGATG GCAATTTGCG CATCACTGCA AATTCTGGAC ATAATCTATA CACTGAGGAC
    TCTTCCTCCT CTTCCACAAG CAGTGATAGT CTAGGGGGGC AGGACCCTCA GCAGCCTAGA
    AAAGGGGAGA CCTGCATTCA AGGGAAGAGA TTGACCAGAC CCACCAGGCT TTCATTGGAA
    AACAGCAGTA AGGCCAGCAC ACTGCCAAGG GCGAGAGAAC CTGCTTTGCC CCCAGAGCCA
    AAAAGTCCTG GGGAGTATGT TAATATAGAG TTTAACGACA AGGTATTTTC AGGGGGCTTG
    ATGCCTTCCA TGTGTCCCCT TCCTTTTGTG CAGAGCAGAG TTGTGCCTCA AAGAGAGAAC
    TTGTCTGAAT ACATGAACAT GGATCTTGGA GTTTGGAGAG CTAAAACTTC CTATGCTTCC
    ACCTCATCTT CATCATATGA ACCACCATAC AAGCCGGTCA GCTCAGTGTG CCCTACTGAA
    ACTTGCAGTA GTAGTCGACC ACCTATTAGA GGAAAGACAA ACTCACGAGA CTATATGAGC
    ATGCAACTTA GTGCTCTGTG TTCAGATTAC AGCCAAGTTC CACCTACCAG AATAACTGCC
    AAACCCATTA CTCTTTCTTC TAATAAAAGT AATTATGCAG AGATGTCAAG TGGCGGAGTT
    TCTGATAATA TTCCTGCTAT TCCCCAAACT TCTAACTCAA GCCTATCTGA AGCATCTCGC
    TCTTCCCTTT TGGGCCAGGG CTCTGGCCCT AGCGCCTTTA CTCGGGTCAG TCTAAGCCCA
    AACCGCAATC AGAGTGCCAA AGTCATCAGG GCCGGGGACC CACAGGGTAG GAGGAGGCAC
    AGCTCAGAGA CATTCTCCTC TACTCCTACT ACAGCCAGAG TTACTTCTGG CCCAGTTTCT
    GGGGAGGATG TCAAGAGACA TAGTTCTGCC TCCTTTGAAA ATGTGTGGCT AAAACCAGGA
    GAGATTGCCA GGAGAGATTC CCTTCAGCCT TCTGATCACA CACATAATGG CCTGAATTAT
    ATTGACTTAG ATCTAGCTAA GGACTTGAGT GGCCTGGATC ATTGCAACTC CCACCAATCT
    GGAGTAAGCC ACCCATCAGA TGACCTGAGC ACATACGCCA GCATCACTTT CCACAAGCTA
    GAGGAGCACC GGAATCAGGC AGAAACAGAA GATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818903.1
    CDS953..4228
    Translation

    Target ORF information:

    RefSeq Version XM_012963449.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis insulin receptor substrate 1 (irs1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963449.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTAGCC CCACAGATCC ACAGGCTCAG GAGAACTTTT CTGATGTCAG AAAGGTGGGC 
    TATCTAAGGA AACCTAAGAG CATGCACAAG AGGTTTTTTG TGCTCAGGTC TGCCAGTGAG
    TCAGGTTTGG CCCGTTTGGA GTATTATGAA AATGAGAAAA AGTGGAGGCA TAAGTCTGGG
    GCCCCAAAGA GGTCAATCCC TCTTGAGAGC TGCTTTAATA TTAACAAGAG GGCTGATTCA
    AAAAACAAAC ATTTGGTTGC TTTGTACACA AAGGAGGAAT GCTTTGCAAT TGCTGCTGAA
    TGTGAACAAG AACAGGATGG TTGGTATCAG GCTCTAGTGG ATCTTCACAA CCGTGGTAAG
    ACCCACCACC AACACCATAA CCATGACGGT GCAACCAATG GTGTACATGA TGGTTTAAAT
    GGAGATGACG TTTATGGGGA GGTTACTCCT CCAGGACTGG CCTTCAAAGA GGTATGGCAG
    GTGATCATGA AGCCCAAGGG TTTAGGGCAA CTTAAGAATT TAGTTGGTAT TTACCGCCTG
    TGCCTCACCA ACCGAACAAT AAGTTTGGTA AAACTGAACT CTGATGCAGC CGCTGTGGTA
    TTGCAACTTA TGAATATAAG GAGATGTGGG CATTCAGAAA ATTTTTTCTT TATAGAAGTG
    GGTCGTTCAG CAGTCACCGG AGCTGGTGAA TTCTGGATGC AAGTGGATGA TTCAGTAGTT
    GCTCAAAACA TGCATGAAAC CATACTAGAA GCTATGAAAG CTCTGAGCGA TGAATTTCGG
    CCTCGGAGTA AGAGTCAGTC TTCATCTAAC TGCTCTAATC CTATATCTGT ACCTCTTAGG
    AGGCATCACC TTAATCATCC ACCACCCAGC CAGGTAGGAC TAAATAGAAG AGCTCGTACA
    GAGAGTGTTA CTGCCACATC ACCTGCAGGG GGTGTGGCAA AGCATGGATC ATCCTCCTCT
    TTCAGAGTCC GGGCATCTAG TGATGGAGAA GGAACAATGT CAAGGCCAGC CTCCATGGAA
    GGGAGCCCAG TAAGTCCCAG TGCTAGCAGA GCTCAGTCGC ACCGGCATCG GGGAAGTTCT
    CGACTTCACC CGCCTCTTAA CCATAGTCGG TCTATACCAA TGCCAGCAAC TCGCTGCTCT
    CCATCGGCTA CCAGCCCTGT CAGCTTGTCC TCCAGTAGTA CCAGTGGACA TGGTTCCACT
    TCTGATTGCA TGTGCCCTCG AAGATCCAGT GCCTCTGTTT CTGGTTCACC CAGTGATGGT
    GGTTTTATTT CTTCAGATGA ATATGGGTCT AGCCCTTGTG ACTTTAGAAG TTCTTTTCGC
    AGTGTAACCC CAGATTCTTT GGGCCACACT CCACCAGCTA GGGAAGAAGA ACTAAATAAC
    TATATTTGCA TGGGCAAATC CGGTAGTCAT CTCCAGAGAA GTCAGCAGCA GAGGTACCAG
    CTAAGTCGAG GGGAAGAGCA CCCTGACTTT GATAAGGTTT TTAGAAAGAG GACTCACTCC
    TCAGGAACAT CACCTCCTAC AGTTTCACAC CAGAAGACAC CATCACAGTC CTCTATTGAG
    GAATATACAG AGATGATGCC TGCTCATCCT GTTCGTTTAA CATCATTTAG ACATTCTGCA
    TTTGTACCCA CCTACTCCTA TCCAGAAGAG TGCCTGGACC TCCATTTAGA GGGTAGCAGA
    GCTAACCACA CAGATGACGG ATACATGCCT ATGTCACCTG GTGTTGCTCC TGTGCCTACA
    AAATCAAATG ACTATATGCC TATGAGCCCT AAGAGTGTGT CGGCCCCTCA ACAGATCATT
    AACCCAAGAC GGCACTCTGC TGTAGATTCA AATGGTTATA TGATGATGTC ACCCAGTGGT
    AGCTGTTCTC CGGATAGCAC CAATTACAGC AAGATATGGA CCAATGGTAC CAATCCAAAG
    TTGTCCATAG ATAGCATTGA GGGCAAACTG CCTTGCAGTG ACTACATTAA TATGTCTCCT
    GCTAGTGGCT CTACTACAAG CACGCCTCCA GACTCTTATC TGAATTCTAT TGAAGAGTCG
    ACCAAGCCTG TATATTCCTA CTTTTCCCTG CCCAGGTCCT TCAAACATGT TCACAGGAAA
    AGTGAAGATG GCAATTTGCG CATCACTGCA AATTCTGGAC ATAATCTATA CACTGAGGAC
    TCTTCCTCCT CTTCCACAAG CAGTGATAGT CTAGGGGGGC AGGACCCTCA GCAGCCTAGA
    AAAGGGGAGA CCTGCATTCA AGGGAAGAGA TTGACCAGAC CCACCAGGCT TTCATTGGAA
    AACAGCAGTA AGGCCAGCAC ACTGCCAAGG GCGAGAGAAC CTGCTTTGCC CCCAGAGCCA
    AAAAGTCCTG GGGAGTATGT TAATATAGAG TTTAACGACA AGGTATTTTC AGGGGGCTTG
    ATGCCTTCCA TGTGTCCCCT TCCTTTTGTG CAGAGCAGAG TTGTGCCTCA AAGAGAGAAC
    TTGTCTGAAT ACATGAACAT GGATCTTGGA GTTTGGAGAG CTAAAACTTC CTATGCTTCC
    ACCTCATCTT CATCATATGA ACCACCATAC AAGCCGGTCA GCTCAGTGTG CCCTACTGAA
    ACTTGCAGTA GTAGTCGACC ACCTATTAGA GGAAAGACAA ACTCACGAGA CTATATGAGC
    ATGCAACTTA GTGCTCTGTG TTCAGATTAC AGCCAAGTTC CACCTACCAG AATAACTGCC
    AAACCCATTA CTCTTTCTTC TAATAAAAGT AATTATGCAG AGATGTCAAG TGGCGGAGTT
    TCTGATAATA TTCCTGCTAT TCCCCAAACT TCTAACTCAA GCCTATCTGA AGCATCTCGC
    TCTTCCCTTT TGGGCCAGGG CTCTGGCCCT AGCGCCTTTA CTCGGGTCAG TCTAAGCCCA
    AACCGCAATC AGAGTGCCAA AGTCATCAGG GCCGGGGACC CACAGGGTAG GAGGAGGCAC
    AGCTCAGAGA CATTCTCCTC TACTCCTACT ACAGCCAGAG TTACTTCTGG CCCAGTTTCT
    GGGGAGGATG TCAAGAGACA TAGTTCTGCC TCCTTTGAAA ATGTGTGGCT AAAACCAGGA
    GAGATTGCCA GGAGAGATTC CCTTCAGCCT TCTGATCACA CACATAATGG CCTGAATTAT
    ATTGACTTAG ATCTAGCTAA GGACTTGAGT GGCCTGGATC ATTGCAACTC CCACCAATCT
    GGAGTAAGCC ACCCATCAGA TGACCTGAGC ACATACGCCA GCATCACTTT CCACAAGCTA
    GAGGAGCACC GGAATCAGGC AGAAACAGAA GATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.