IRS1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following IRS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe25730 XM_543274.6
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Canis lupus familiaris insulin receptor substrate 1 (IRS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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OCe25730 XM_543274.5 Canis lupus familiaris insulin receptor substrate 1 (IRS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe25730
    Clone ID Related Accession (Same CDS sequence) XM_543274.6 , XM_543274.5
    Accession Version XM_543274.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LOW QUALITY PROTEIN: insulin receptor substrate 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_543274.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAGCC CTCCGGAGAC CGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA GCGCTTCTTC GTGCTGCGGG CGGCCAGCGA GGCGGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC
    AAACGCTCGA TCCCCCTCGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTTTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGGGC CAAGGGCCAC
    CACGACGGCG CCTCGGCCCC CGGGGCGGGA GGCGGCGGGG GCAGCTGCAG CGGCAGCTCG
    GGCCTCGGGG AGGCCGGCGA GGACTTGAGC TACGGGGACG TGCCCCCGGG ACCTGCGTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GGCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCCGAG
    GCGGCGGCCG TGGTGCTGCA GCTGATGAAC ATCCGACGTT GCGGCCACTC GGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGACCCG GCGAGTTCTG GATGCAGGTG
    GATGACTCCG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG
    AGCGACGAGT TCCGCCCTCG GAGTAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGCGTCCCCC TGCGCCGGCA CCACCTCAAC AACCCCCCTC CCAGCCAGGT GGGGCTGACG
    CGCCGCTCGC GCACCGAGAG CATCACCGCC ACCTCTCCGG CCAGCATGGT GGGCGGGAAG
    CAGGGCTCCT TCCGTGTGCG CGCGTCCAGC GACGGCGAGG GCACCATGTC CCGCCCGGCC
    TCGGTGGACG GCAGCCCCGT GAGCCCGAGC ACCACCAGGA CCCACGCGCA CCGGCATCGC
    GGCAGCTCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCCTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCGGTC AGCCTGTCGT CCAGCAGCAC CAGTGGCCAC
    GGCTCCACCT CGGACTGCCT CTTCCCCCGG CGCTCTAGTG CCTCTGTGTC GGGTTCCCCC
    AGCGACGGTG GTTTCATCTC CTCTGACGAG TACGGCTCGA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GTGTCACCCC GGATTCCCTG GGCCACACCC CCCCGGCCCG CGGCGAGGAG
    GAGCTGAGCA ACTACATCTG CATGGGAGGC AAAGGGTCCT CCACCCTCAC CGCCCCCAAC
    GGTCACTACA TTTTGCCTCG GGGTGGCAAT GGCCACCGCT ACATCCCGGG GGCTGGCTTG
    GGCACCAGCC CGGCCCTGGC TGCGGATGAA GCGGCCGCTG CGGCCGACCT GGATAACCGG
    TTCCGAAAGC GGACTCACTC CGCGGGCACA TCCCCTACCA TTTCCCACCA GAAGACCCCG
    TCCCAGTCTT CTGTGGCTTC CATTGAGGAG TACACGGAGA TGATGCCTGC CTACCCGCCA
    GGAGGTGGCA GTGGAGGCCG ACTGCCTGGC TACCGGCACT CTGCCTTCGT GCCCACCCAC
    TCCTACCCCG AGGAGGGTCT GGAAATGCAC CCTCTGGACA GGCGTGGGGG CCACCACCGG
    CCGGACGCCG CCGCCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC GGGAGTGGCA
    CCGGTGCCCA GCAGCCGGAA GGGCAGTGGG GACTATATGC CCATGAGCCC CAAGAGCGTG
    TCCGCGCCGC AGCAGATCAT CAACCCCATT AGACGCCATC CCCAGAGGGT GGACCCCAAT
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCGGC
    AGCAGCAGCA GCGGCAGCGC CGCCCCTTCT GGGAGCAGCT ATGGCAAGCT GTGGACAAAC
    GGGGTAGGGG GCCACCACCC TCACGCCCTG CCGCACCCCA AACTCCCCGT GGAGAGCGGG
    AGTGGCAAGC TCCTGTCTTG TACCGGCGAC TACATGAACA TGTCGCCGGT GGGGGACTCC
    AACACCAGCA GCCCCTCCGA CGGCTACTAC GGCCCAGAGG ACCCCCAGCA CAAGCCAGTT
    CTCTCCTACT ACTCATTGCC AAGGTCCTTT AAGCACACCC AGCGCCCTGG GGAGCTGGAG
    GAGAGCGCCC GGCACNAGCA CCTCCGCCTC TCCTCCAGCT CGGGTCGTCT TCTCTACGCC
    GCGACGGCGG AAGATTCCTC CTCCTCCACC AGCAGCGACA GCCTGGGCCC AGGGGGATAC
    TGTGGGGTCA GGCCGGATCC CGGCCTCCCG CATATCCACC ATCAGGTCCT GCAGCCTCAC
    CTGCCTCGGA AGGTGGACAC GGCCGCGCAG ACCAACAGCC GCCTGGCTCG GCCCACGAGG
    CTGTCCCTGG GGGACCCCAA GGCCAGCACC TTACCTCGGG TTCGAGAGCA GCAGCACCCG
    CCGCCCCTGC TGCACCCTCC GGAGCCCAAG AGCCCCGGGG AATATGTGAA TATTGAGTTC
    GGGAGCGATC AGCCGGGCTA CTTATCGGGG CCGGTGGCTG CCCGCAGCTC GCCTTCTGTC
    AGGTGCCCAC CCCAGCTCCA GCCAGCTCCC CGCGAGGAAG AGACTGGCAC CGAGGAGTAC
    ATGAACATGG ACCTGGGGCC TGGCCGGAGG GCAGCCTGGC AGGAGGGTGC TGGGGTCCAG
    CCCGGCAGGG TGGGCCCCGC GCCCCCCGGG GCCGCTAGCG TGTGCAGGCC CACCCGGGCA
    GTGCCCAGCA GCCGGGGCGA CTACATGACC ATGCAGGTGG GCTGTCCCGG CCAGGGCTAC
    GTGGACACCT CGCCAGTGGC CCCCATCAGC TACGCTGACA TGCGGACAGG CATTGTCGTG
    GAGGAGGCCA GCCTGCCGGG GGCCACAGCG GCCGCCCCCT CCTCGGCCTC GGCAGCCTCG
    GCTTCCCCCA CGGCGCCTCC AAAAGCGGGG GAGCTGGTGG CCCGCTCCTC CCTGCTGGGG
    GGCCCGCAGG GACCCGGGGG CATGAGCGCC TTCACCCGGG TGAACCTCAG CCCCAACCGC
    AACCAGAGTG CCAAAGTGAT CCGCGCCGAC CCGCAGGGGT GCCGGAGGCG GCATAGCTCT
    GAGACCTTCT CCTCCACGCC CAGTGCCACC CGGGCGGGCA ACGCAGTGCC CTTCGGCGGG
    GGGGCGGCCC TGGGGGGCAG CGGTGGCGGC AGCAGCGCGG AGGATATGAA ACGCCACAGT
    TCGGCTTCCT TTGAGAACGT GTGGCTGAGG CCTGGGGAGC TCGGGGGAGC CCCCAAGGAG
    CCGGCCCCGC ACGCTGGGGC CGCCGGGGGT TTGGAGAATG GGCTTAACTA CATAGACCTG
    GATTTGGTCA AGGACTTCAA ACAGTGCTCT CAGGAGCGCC CCCCTCAACC GCAGCCGCCC
    CCGCCCCCGG CCCCTCATCA GCCTCTGGGC AGCAGTGAGA GCAGTTCAAC CAGCCGCTCC
    AGCGAGGATC TAAGCGCCTA TGCCAGCATC AGTTTCCAGA AGCAGCCAGA GGACCTCCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543274.3
    CDS21..3743
    Translation

    Target ORF information:

    RefSeq Version XM_543274.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris insulin receptor substrate 1 (IRS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543274.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAGCC CTCCGGAGAC CGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA GCGCTTCTTC GTGCTGCGGG CGGCCAGCGA GGCGGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC
    AAACGCTCGA TCCCCCTCGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTTTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGGGC CAAGGGCCAC
    CACGACGGCG CCTCGGCCCC CGGGGCGGGA GGCGGCGGGG GCAGCTGCAG CGGCAGCTCG
    GGCCTCGGGG AGGCCGGCGA GGACTTGAGC TACGGGGACG TGCCCCCGGG ACCTGCGTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GGCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCCGAG
    GCGGCGGCCG TGGTGCTGCA GCTGATGAAC ATCCGACGTT GCGGCCACTC GGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGACCCG GCGAGTTCTG GATGCAGGTG
    GATGACTCCG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG
    AGCGACGAGT TCCGCCCTCG GAGTAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGCGTCCCCC TGCGCCGGCA CCACCTCAAC AACCCCCCTC CCAGCCAGGT GGGGCTGACG
    CGCCGCTCGC GCACCGAGAG CATCACCGCC ACCTCTCCGG CCAGCATGGT GGGCGGGAAG
    CAGGGCTCCT TCCGTGTGCG CGCGTCCAGC GACGGCGAGG GCACCATGTC CCGCCCGGCC
    TCGGTGGACG GCAGCCCCGT GAGCCCGAGC ACCACCAGGA CCCACGCGCA CCGGCATCGC
    GGCAGCTCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCCTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCGGTC AGCCTGTCGT CCAGCAGCAC CAGTGGCCAC
    GGCTCCACCT CGGACTGCCT CTTCCCCCGG CGCTCTAGTG CCTCTGTGTC GGGTTCCCCC
    AGCGACGGTG GTTTCATCTC CTCTGACGAG TACGGCTCGA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GTGTCACCCC GGATTCCCTG GGCCACACCC CCCCGGCCCG CGGCGAGGAG
    GAGCTGAGCA ACTACATCTG CATGGGAGGC AAAGGGTCCT CCACCCTCAC CGCCCCCAAC
    GGTCACTACA TTTTGCCTCG GGGTGGCAAT GGCCACCGCT ACATCCCGGG GGCTGGCTTG
    GGCACCAGCC CGGCCCTGGC TGCGGATGAA GCGGCCGCTG CGGCCGACCT GGATAACCGG
    TTCCGAAAGC GGACTCACTC CGCGGGCACA TCCCCTACCA TTTCCCACCA GAAGACCCCG
    TCCCAGTCTT CTGTGGCTTC CATTGAGGAG TACACGGAGA TGATGCCTGC CTACCCGCCA
    GGAGGTGGCA GTGGAGGCCG ACTGCCTGGC TACCGGCACT CTGCCTTCGT GCCCACCCAC
    TCCTACCCCG AGGAGGGTCT GGAAATGCAC CCTCTGGACA GGCGTGGGGG CCACCACCGG
    CCGGACGCCG CCGCCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC GGGAGTGGCA
    CCGGTGCCCA GCAGCCGGAA GGGCAGTGGG GACTATATGC CCATGAGCCC CAAGAGCGTG
    TCCGCGCCGC AGCAGATCAT CAACCCCATT AGACGCCATC CCCAGAGGGT GGACCCCAAT
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCGGC
    AGCAGCAGCA GCGGCAGCGC CGCCCCTTCT GGGAGCAGCT ATGGCAAGCT GTGGACAAAC
    GGGGTAGGGG GCCACCACCC TCACGCCCTG CCGCACCCCA AACTCCCCGT GGAGAGCGGG
    AGTGGCAAGC TCCTGTCTTG TACCGGCGAC TACATGAACA TGTCGCCGGT GGGGGACTCC
    AACACCAGCA GCCCCTCCGA CGGCTACTAC GGCCCAGAGG ACCCCCAGCA CAAGCCAGTT
    CTCTCCTACT ACTCATTGCC AAGGTCCTTT AAGCACACCC AGCGCCCTGG GGAGCTGGAG
    GAGAGCGCCC GGCACNAGCA CCTCCGCCTC TCCTCCAGCT CGGGTCGTCT TCTCTACGCC
    GCGACGGCGG AAGATTCCTC CTCCTCCACC AGCAGCGACA GCCTGGGCCC AGGGGGATAC
    TGTGGGGTCA GGCCGGATCC CGGCCTCCCG CATATCCACC ATCAGGTCCT GCAGCCTCAC
    CTGCCTCGGA AGGTGGACAC GGCCGCGCAG ACCAACAGCC GCCTGGCTCG GCCCACGAGG
    CTGTCCCTGG GGGACCCCAA GGCCAGCACC TTACCTCGGG TTCGAGAGCA GCAGCACCCG
    CCGCCCCTGC TGCACCCTCC GGAGCCCAAG AGCCCCGGGG AATATGTGAA TATTGAGTTC
    GGGAGCGATC AGCCGGGCTA CTTATCGGGG CCGGTGGCTG CCCGCAGCTC GCCTTCTGTC
    AGGTGCCCAC CCCAGCTCCA GCCAGCTCCC CGCGAGGAAG AGACTGGCAC CGAGGAGTAC
    ATGAACATGG ACCTGGGGCC TGGCCGGAGG GCAGCCTGGC AGGAGGGTGC TGGGGTCCAG
    CCCGGCAGGG TGGGCCCCGC GCCCCCCGGG GCCGCTAGCG TGTGCAGGCC CACCCGGGCA
    GTGCCCAGCA GCCGGGGCGA CTACATGACC ATGCAGGTGG GCTGTCCCGG CCAGGGCTAC
    GTGGACACCT CGCCAGTGGC CCCCATCAGC TACGCTGACA TGCGGACAGG CATTGTCGTG
    GAGGAGGCCA GCCTGCCGGG GGCCACAGCG GCCGCCCCCT CCTCGGCCTC GGCAGCCTCG
    GCTTCCCCCA CGGCGCCTCC AAAAGCGGGG GAGCTGGTGG CCCGCTCCTC CCTGCTGGGG
    GGCCCGCAGG GACCCGGGGG CATGAGCGCC TTCACCCGGG TGAACCTCAG CCCCAACCGC
    AACCAGAGTG CCAAAGTGAT CCGCGCCGAC CCGCAGGGGT GCCGGAGGCG GCATAGCTCT
    GAGACCTTCT CCTCCACGCC CAGTGCCACC CGGGCGGGCA ACGCAGTGCC CTTCGGCGGG
    GGGGCGGCCC TGGGGGGCAG CGGTGGCGGC AGCAGCGCGG AGGATATGAA ACGCCACAGT
    TCGGCTTCCT TTGAGAACGT GTGGCTGAGG CCTGGGGAGC TCGGGGGAGC CCCCAAGGAG
    CCGGCCCCGC ACGCTGGGGC CGCCGGGGGT TTGGAGAATG GGCTTAACTA CATAGACCTG
    GATTTGGTCA AGGACTTCAA ACAGTGCTCT CAGGAGCGCC CCCCTCAACC GCAGCCGCCC
    CCGCCCCCGG CCCCTCATCA GCCTCTGGGC AGCAGTGAGA GCAGTTCAAC CAGCCGCTCC
    AGCGAGGATC TAAGCGCCTA TGCCAGCATC AGTTTCCAGA AGCAGCCAGA GGACCTCCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25730
    Clone ID Related Accession (Same CDS sequence) XM_543274.6 , XM_543274.5
    Accession Version XM_543274.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LOW QUALITY PROTEIN: insulin receptor substrate 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_543274.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAGCC CTCCGGAGAC CGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA GCGCTTCTTC GTGCTGCGGG CGGCCAGCGA GGCGGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC
    AAACGCTCGA TCCCCCTCGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTTTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGGGC CAAGGGCCAC
    CACGACGGCG CCTCGGCCCC CGGGGCGGGA GGCGGCGGGG GCAGCTGCAG CGGCAGCTCG
    GGCCTCGGGG AGGCCGGCGA GGACTTGAGC TACGGGGACG TGCCCCCGGG ACCTGCGTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GGCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCCGAG
    GCGGCGGCCG TGGTGCTGCA GCTGATGAAC ATCCGACGTT GCGGCCACTC GGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGACCCG GCGAGTTCTG GATGCAGGTG
    GATGACTCCG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG
    AGCGACGAGT TCCGCCCTCG GAGTAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGCGTCCCCC TGCGCCGGCA CCACCTCAAC AACCCCCCTC CCAGCCAGGT GGGGCTGACG
    CGCCGCTCGC GCACCGAGAG CATCACCGCC ACCTCTCCGG CCAGCATGGT GGGCGGGAAG
    CAGGGCTCCT TCCGTGTGCG CGCGTCCAGC GACGGCGAGG GCACCATGTC CCGCCCGGCC
    TCGGTGGACG GCAGCCCCGT GAGCCCGAGC ACCACCAGGA CCCACGCGCA CCGGCATCGC
    GGCAGCTCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCCTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCGGTC AGCCTGTCGT CCAGCAGCAC CAGTGGCCAC
    GGCTCCACCT CGGACTGCCT CTTCCCCCGG CGCTCTAGTG CCTCTGTGTC GGGTTCCCCC
    AGCGACGGTG GTTTCATCTC CTCTGACGAG TACGGCTCGA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GTGTCACCCC GGATTCCCTG GGCCACACCC CCCCGGCCCG CGGCGAGGAG
    GAGCTGAGCA ACTACATCTG CATGGGAGGC AAAGGGTCCT CCACCCTCAC CGCCCCCAAC
    GGTCACTACA TTTTGCCTCG GGGTGGCAAT GGCCACCGCT ACATCCCGGG GGCTGGCTTG
    GGCACCAGCC CGGCCCTGGC TGCGGATGAA GCGGCCGCTG CGGCCGACCT GGATAACCGG
    TTCCGAAAGC GGACTCACTC CGCGGGCACA TCCCCTACCA TTTCCCACCA GAAGACCCCG
    TCCCAGTCTT CTGTGGCTTC CATTGAGGAG TACACGGAGA TGATGCCTGC CTACCCGCCA
    GGAGGTGGCA GTGGAGGCCG ACTGCCTGGC TACCGGCACT CTGCCTTCGT GCCCACCCAC
    TCCTACCCCG AGGAGGGTCT GGAAATGCAC CCTCTGGACA GGCGTGGGGG CCACCACCGG
    CCGGACGCCG CCGCCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC GGGAGTGGCA
    CCGGTGCCCA GCAGCCGGAA GGGCAGTGGG GACTATATGC CCATGAGCCC CAAGAGCGTG
    TCCGCGCCGC AGCAGATCAT CAACCCCATT AGACGCCATC CCCAGAGGGT GGACCCCAAT
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCGGC
    AGCAGCAGCA GCGGCAGCGC CGCCCCTTCT GGGAGCAGCT ATGGCAAGCT GTGGACAAAC
    GGGGTAGGGG GCCACCACCC TCACGCCCTG CCGCACCCCA AACTCCCCGT GGAGAGCGGG
    AGTGGCAAGC TCCTGTCTTG TACCGGCGAC TACATGAACA TGTCGCCGGT GGGGGACTCC
    AACACCAGCA GCCCCTCCGA CGGCTACTAC GGCCCAGAGG ACCCCCAGCA CAAGCCAGTT
    CTCTCCTACT ACTCATTGCC AAGGTCCTTT AAGCACACCC AGCGCCCTGG GGAGCTGGAG
    GAGAGCGCCC GGCACNAGCA CCTCCGCCTC TCCTCCAGCT CGGGTCGTCT TCTCTACGCC
    GCGACGGCGG AAGATTCCTC CTCCTCCACC AGCAGCGACA GCCTGGGCCC AGGGGGATAC
    TGTGGGGTCA GGCCGGATCC CGGCCTCCCG CATATCCACC ATCAGGTCCT GCAGCCTCAC
    CTGCCTCGGA AGGTGGACAC GGCCGCGCAG ACCAACAGCC GCCTGGCTCG GCCCACGAGG
    CTGTCCCTGG GGGACCCCAA GGCCAGCACC TTACCTCGGG TTCGAGAGCA GCAGCACCCG
    CCGCCCCTGC TGCACCCTCC GGAGCCCAAG AGCCCCGGGG AATATGTGAA TATTGAGTTC
    GGGAGCGATC AGCCGGGCTA CTTATCGGGG CCGGTGGCTG CCCGCAGCTC GCCTTCTGTC
    AGGTGCCCAC CCCAGCTCCA GCCAGCTCCC CGCGAGGAAG AGACTGGCAC CGAGGAGTAC
    ATGAACATGG ACCTGGGGCC TGGCCGGAGG GCAGCCTGGC AGGAGGGTGC TGGGGTCCAG
    CCCGGCAGGG TGGGCCCCGC GCCCCCCGGG GCCGCTAGCG TGTGCAGGCC CACCCGGGCA
    GTGCCCAGCA GCCGGGGCGA CTACATGACC ATGCAGGTGG GCTGTCCCGG CCAGGGCTAC
    GTGGACACCT CGCCAGTGGC CCCCATCAGC TACGCTGACA TGCGGACAGG CATTGTCGTG
    GAGGAGGCCA GCCTGCCGGG GGCCACAGCG GCCGCCCCCT CCTCGGCCTC GGCAGCCTCG
    GCTTCCCCCA CGGCGCCTCC AAAAGCGGGG GAGCTGGTGG CCCGCTCCTC CCTGCTGGGG
    GGCCCGCAGG GACCCGGGGG CATGAGCGCC TTCACCCGGG TGAACCTCAG CCCCAACCGC
    AACCAGAGTG CCAAAGTGAT CCGCGCCGAC CCGCAGGGGT GCCGGAGGCG GCATAGCTCT
    GAGACCTTCT CCTCCACGCC CAGTGCCACC CGGGCGGGCA ACGCAGTGCC CTTCGGCGGG
    GGGGCGGCCC TGGGGGGCAG CGGTGGCGGC AGCAGCGCGG AGGATATGAA ACGCCACAGT
    TCGGCTTCCT TTGAGAACGT GTGGCTGAGG CCTGGGGAGC TCGGGGGAGC CCCCAAGGAG
    CCGGCCCCGC ACGCTGGGGC CGCCGGGGGT TTGGAGAATG GGCTTAACTA CATAGACCTG
    GATTTGGTCA AGGACTTCAA ACAGTGCTCT CAGGAGCGCC CCCCTCAACC GCAGCCGCCC
    CCGCCCCCGG CCCCTCATCA GCCTCTGGGC AGCAGTGAGA GCAGTTCAAC CAGCCGCTCC
    AGCGAGGATC TAAGCGCCTA TGCCAGCATC AGTTTCCAGA AGCAGCCAGA GGACCTCCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543274.3
    CDS81..3803
    Translation

    Target ORF information:

    RefSeq Version XM_543274.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris insulin receptor substrate 1 (IRS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543274.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAGCC CTCCGGAGAC CGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
    AAACCCAAGA GCATGCACAA GCGCTTCTTC GTGCTGCGGG CGGCCAGCGA GGCGGGGGGC
    CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC
    AAACGCTCGA TCCCCCTCGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC
    AAGCACCTGG TGGCCCTTTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG
    GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGGGC CAAGGGCCAC
    CACGACGGCG CCTCGGCCCC CGGGGCGGGA GGCGGCGGGG GCAGCTGCAG CGGCAGCTCG
    GGCCTCGGGG AGGCCGGCGA GGACTTGAGC TACGGGGACG TGCCCCCGGG ACCTGCGTTC
    AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GGCAGACAAA GAACCTGATT
    GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCCGAG
    GCGGCGGCCG TGGTGCTGCA GCTGATGAAC ATCCGACGTT GCGGCCACTC GGAGAACTTC
    TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGACCCG GCGAGTTCTG GATGCAGGTG
    GATGACTCCG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG
    AGCGACGAGT TCCGCCCTCG GAGTAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC
    AGCGTCCCCC TGCGCCGGCA CCACCTCAAC AACCCCCCTC CCAGCCAGGT GGGGCTGACG
    CGCCGCTCGC GCACCGAGAG CATCACCGCC ACCTCTCCGG CCAGCATGGT GGGCGGGAAG
    CAGGGCTCCT TCCGTGTGCG CGCGTCCAGC GACGGCGAGG GCACCATGTC CCGCCCGGCC
    TCGGTGGACG GCAGCCCCGT GAGCCCGAGC ACCACCAGGA CCCACGCGCA CCGGCATCGC
    GGCAGCTCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCCTCT
    CGCTGCTCGC CTTCCGCCAC CAGCCCGGTC AGCCTGTCGT CCAGCAGCAC CAGTGGCCAC
    GGCTCCACCT CGGACTGCCT CTTCCCCCGG CGCTCTAGTG CCTCTGTGTC GGGTTCCCCC
    AGCGACGGTG GTTTCATCTC CTCTGACGAG TACGGCTCGA GTCCCTGCGA TTTCCGAAGT
    TCCTTCCGCA GTGTCACCCC GGATTCCCTG GGCCACACCC CCCCGGCCCG CGGCGAGGAG
    GAGCTGAGCA ACTACATCTG CATGGGAGGC AAAGGGTCCT CCACCCTCAC CGCCCCCAAC
    GGTCACTACA TTTTGCCTCG GGGTGGCAAT GGCCACCGCT ACATCCCGGG GGCTGGCTTG
    GGCACCAGCC CGGCCCTGGC TGCGGATGAA GCGGCCGCTG CGGCCGACCT GGATAACCGG
    TTCCGAAAGC GGACTCACTC CGCGGGCACA TCCCCTACCA TTTCCCACCA GAAGACCCCG
    TCCCAGTCTT CTGTGGCTTC CATTGAGGAG TACACGGAGA TGATGCCTGC CTACCCGCCA
    GGAGGTGGCA GTGGAGGCCG ACTGCCTGGC TACCGGCACT CTGCCTTCGT GCCCACCCAC
    TCCTACCCCG AGGAGGGTCT GGAAATGCAC CCTCTGGACA GGCGTGGGGG CCACCACCGG
    CCGGACGCCG CCGCCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC GGGAGTGGCA
    CCGGTGCCCA GCAGCCGGAA GGGCAGTGGG GACTATATGC CCATGAGCCC CAAGAGCGTG
    TCCGCGCCGC AGCAGATCAT CAACCCCATT AGACGCCATC CCCAGAGGGT GGACCCCAAT
    GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCGGC
    AGCAGCAGCA GCGGCAGCGC CGCCCCTTCT GGGAGCAGCT ATGGCAAGCT GTGGACAAAC
    GGGGTAGGGG GCCACCACCC TCACGCCCTG CCGCACCCCA AACTCCCCGT GGAGAGCGGG
    AGTGGCAAGC TCCTGTCTTG TACCGGCGAC TACATGAACA TGTCGCCGGT GGGGGACTCC
    AACACCAGCA GCCCCTCCGA CGGCTACTAC GGCCCAGAGG ACCCCCAGCA CAAGCCAGTT
    CTCTCCTACT ACTCATTGCC AAGGTCCTTT AAGCACACCC AGCGCCCTGG GGAGCTGGAG
    GAGAGCGCCC GGCACNAGCA CCTCCGCCTC TCCTCCAGCT CGGGTCGTCT TCTCTACGCC
    GCGACGGCGG AAGATTCCTC CTCCTCCACC AGCAGCGACA GCCTGGGCCC AGGGGGATAC
    TGTGGGGTCA GGCCGGATCC CGGCCTCCCG CATATCCACC ATCAGGTCCT GCAGCCTCAC
    CTGCCTCGGA AGGTGGACAC GGCCGCGCAG ACCAACAGCC GCCTGGCTCG GCCCACGAGG
    CTGTCCCTGG GGGACCCCAA GGCCAGCACC TTACCTCGGG TTCGAGAGCA GCAGCACCCG
    CCGCCCCTGC TGCACCCTCC GGAGCCCAAG AGCCCCGGGG AATATGTGAA TATTGAGTTC
    GGGAGCGATC AGCCGGGCTA CTTATCGGGG CCGGTGGCTG CCCGCAGCTC GCCTTCTGTC
    AGGTGCCCAC CCCAGCTCCA GCCAGCTCCC CGCGAGGAAG AGACTGGCAC CGAGGAGTAC
    ATGAACATGG ACCTGGGGCC TGGCCGGAGG GCAGCCTGGC AGGAGGGTGC TGGGGTCCAG
    CCCGGCAGGG TGGGCCCCGC GCCCCCCGGG GCCGCTAGCG TGTGCAGGCC CACCCGGGCA
    GTGCCCAGCA GCCGGGGCGA CTACATGACC ATGCAGGTGG GCTGTCCCGG CCAGGGCTAC
    GTGGACACCT CGCCAGTGGC CCCCATCAGC TACGCTGACA TGCGGACAGG CATTGTCGTG
    GAGGAGGCCA GCCTGCCGGG GGCCACAGCG GCCGCCCCCT CCTCGGCCTC GGCAGCCTCG
    GCTTCCCCCA CGGCGCCTCC AAAAGCGGGG GAGCTGGTGG CCCGCTCCTC CCTGCTGGGG
    GGCCCGCAGG GACCCGGGGG CATGAGCGCC TTCACCCGGG TGAACCTCAG CCCCAACCGC
    AACCAGAGTG CCAAAGTGAT CCGCGCCGAC CCGCAGGGGT GCCGGAGGCG GCATAGCTCT
    GAGACCTTCT CCTCCACGCC CAGTGCCACC CGGGCGGGCA ACGCAGTGCC CTTCGGCGGG
    GGGGCGGCCC TGGGGGGCAG CGGTGGCGGC AGCAGCGCGG AGGATATGAA ACGCCACAGT
    TCGGCTTCCT TTGAGAACGT GTGGCTGAGG CCTGGGGAGC TCGGGGGAGC CCCCAAGGAG
    CCGGCCCCGC ACGCTGGGGC CGCCGGGGGT TTGGAGAATG GGCTTAACTA CATAGACCTG
    GATTTGGTCA AGGACTTCAA ACAGTGCTCT CAGGAGCGCC CCCCTCAACC GCAGCCGCCC
    CCGCCCCCGG CCCCTCATCA GCCTCTGGGC AGCAGTGAGA GCAGTTCAAC CAGCCGCTCC
    AGCGAGGATC TAAGCGCCTA TGCCAGCATC AGTTTCCAGA AGCAGCCAGA GGACCTCCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.