NFAT5 cDNA ORF clone, Bos taurus(cattle)

The following NFAT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFAT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa28567 XM_010823632.2
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa28567 XM_003583392.4
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa28567 XM_005218872.3
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa28567 XM_010814694.2
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa28568 XM_005218873.3
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa28568 XM_005195289.3
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa28568 XM_010814695.2
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa28568 XM_010823634.2
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa28568 XM_005195290.3
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa28568 XM_005218874.3
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa53919 XM_010814696.2
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa53919 XM_015467397.1
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa93417 XM_015467395.1
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa93417 XM_015475835.1
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa225503 XM_002694839.6
Latest version!
Bos taurus nuclear factor of activated T cells 5 (NFAT5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa28565 XM_002694839.5
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa28565 XM_015467392.1
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa28566 XM_003587226.4
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa28566 XM_015467394.1
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa53918 XM_015467393.1
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa53918 XM_010814692.2
Latest version!
Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa28567
    Clone ID Related Accession (Same CDS sequence) XM_010814694.2 , XM_010823632.2 , XM_005218872.3 , XM_003583392.4
    Accession Version XM_010814694.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
    GTCACCGACA GCAAGGCTAT GCAAGTCGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC
    CGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG
    AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG
    ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG
    GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG
    GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT
    TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG
    CTTTCTCAGC TTACCACGGA CAACAAAGGC AGCTCAAAAG CAGGAAATGG AACATTGGAA
    AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTAT GTGGACAGTA TCCTGTTAAA
    AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CCCAGCACAG AGCTCGGTAC
    CTGACTGAGG GTAGTCGTGG CTCAGTAAAG GACAGAACAC AGCAAGGCTT TCCTACAGTG
    AAGCTGGAAG GCCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT
    CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGGGTAA CTGGGCGAAA TACAACTCCC
    TGCAAAGAAG TGGATATCGA AGGCACTACT GTTATAGAAG TTGGCCTTGA TCCAAGCAAC
    AATATGACAC TGGCGGTTGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTTGAA
    GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA
    GTTAATATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT
    TTATGTACTC AACCAGCAGG AGTGCCAGAA ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG
    GTGAAAGGAG AGGAAGAAGT ATTTTTAATT GGCAAGAACT TTCTGAAAGG AACTAAAGTT
    ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG
    GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA TCAACATATA
    ACTTTGCCAG TAGCAGTGGG AATATATGTA GTAACCAATG CTGGAAGATC TCATGATGTT
    CAGCCTTTCA CTTACACTCC AGACCCAGCA GCTGTTGCTT TGAATGTGAA TGTAAAGAAG
    GAAATATCTA GTCCGGCAAG ACCTTGCTCT TTCGAAGAAG CCATGAAAGC AATGAAAACT
    ACTGGATGTA ACTTAGATAA GGTAAATATG CTTCCTAATG CTCTGATCAC TCCGCTCATA
    TCAAGCACTA TGATTAAGAG TGAAGACATT ACTCCAATGG AAGTAACAGC AGAGAAAAGA
    TCGCCTTCTA TTTTTAAGAC TACAAAGACA GTTGGATCAA CTCAACAAAC GTTAGAAAAT
    CTCTCTCACA TAGCAGGAAA TGGGTCTTTT TCATCATCAT CTTCCCACTT AACTTCTGAA
    AATGAAAAGC AACAACAGAT TCAGCCCAAG GCATACAACC CAGAGACCCT GACAACTATC
    CAAACACAGG ACATTTCACA GCCTGGTACT TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG
    CCCAGCAATG ACGCACTACT ACAGCAGGCT ACACAGTTTC AAACAAGAGA AACTCAGTCT
    AGAGAGGTGT TACAGTCAGA TGGTACAGTG GTTAACTTGT CACACCTGAC TGAGACATCA
    CAGCAACAGC AGCAGTCTCC ACTGCAAGAA CAAGCACAGA CTTTACAGCA GCAGATTTCA
    TCAAATATTT TCCCATCACC AAATAGTGTG AGTCAGCAAC TACAAAATAC GATACAACAC
    TTGCAGGCAG GGAGTTTTAC AGGCAGTACT GCCAGTGGCA GCAATGGAAA TGTTGACTTG
    GTCCAACAAG TTTTAGAGGC ACAACAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT
    GGTAACGAGA ATGTTCAAGA ACAGCTTAGT GCAGACATTT TTCAACAAGT CAGTCAAATT
    CAAAATAGCG TAAGCCCTGG AATGTTTTCC TCAACAGAAC CAGCAGTCCA TACCAGACCA
    GATAATTTAA TAGCTGGAAG AGCTGAAAGT GTTCACCCAC AGAATGAAAA CACATTGTCT
    AATCAGCAAC AGCAACAGCA GCAACAGCAA GTGATGGACT CCTCAGCTGC AATGGTGATG
    GAGATGCAAC AGAGTATCTG CCAGGCAGCT GCCCAGATTC AGTCAGAGCT GTTTCCTTCA
    TCTGCTTCAG CAAATGGAAA CCTTCAGCAG TCTCCAGTTT ACCAGCAGAC TTCTCATATG
    ATGAGCGCAT TATCAGCCAA TGAGGACATG CAAATGCAAT GTGAATTGTT TTCTTCTCCT
    CCGGCAGTTT CTGGAAATGA AACTACTACA ACTACCACAC AGCAGGTTGC AACTTCTGGC
    ACTACCCTGT TTCAGACATC AAATTCAGGA GATGGAGAAG AGACTGGAGC ACAAGCAAAA
    CAGATTCAGA ACAGTGTCTT TCAGACCATG GTCCAAATGC AACATAGTGG GGACAGTCAG
    CCTCAAGTTG GCCTTTTTTC ATCTACAAAA AGTATGATAA GTGTTCAAAA TAGTGGTACC
    CAGCAGCAAG GCAATGGTTT ATTCCAGCAA GGTAATGAGA TGATGTCACT TCAATCAGGG
    AATTTTTTGC AGCAGTCTTC TCATTCACAG GCTCAGCTTT TTCATCCTCA GAATCCTATT
    GCTGATCCTC AGAACCTTTC CCAGGAAACT CAAGGTTCTA TTTTTCATAG TCCAAGTCCT
    ATTGTCCACA GTCAGACTTC TACAGCCTCC TCTGAACAAA TGCAGCCTCC AATGTTTCAC
    TCTCAAAATA CCATGGCTGT GCTGCAGGGC TCTTCTGTTC CTCAAGACCA ACAGTCAGCC
    AACATATTTC TTTCCCAAAG TCCAATGAAT AATCTCCAAA CCAACACAGT GGCCCAGGAA
    GAACAGATTT CATTTTTTGC AGCTCAGAAT TCAATTTCTC CACTTCAGTC AACATCAAAC
    ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG GCTCCAATAT CACACATTCA GACCCCTATG
    CTTTCCCAGG AACAGGCACA ACCCTCCCAG CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG
    GGCTCCCTTC CTCCTAACCC AATGCCTCAA AACCAACAAG GAACAATCTT TCAGTCACAG
    CACTCAATAG TTGCCATCCA GAGTAACTCT CCATCCCAGG AGCAGCAGCA GCAGCAACAA
    CAGCAGCAGC AACAACAGAG CATTTTATTC AGTAACCAGA ACGCCATGGC ACCAATGGCG
    TCTCAAAAGC AGCCACCACC AAACATGATA TTCAATCCAA GTCAAAATCC AGTGGCTAAT
    CAGGAGCAGC AGAACCAATC AATTTTCCAT CAACAAAATA ATATGGCCCC AATGAATCAA
    GAGCAGCAGC CCATGCAATT TCAGAACCAG ACCACGGTTT CCTCACTTCA GAACCCAGGT
    CCTGCCCAAT CCGAATCATC ACAGACCTCC TTGTTCCATA GCTCACCTCA GATTCAGTTG
    GTCCAAGGGT CACCCAGTTC TCAAGAGCAG CAAGTAACAC TCTTCCTCTC TCCAGCATCC
    ATGTCTGCAT TGCAGACCAG TATGAACCAA CAAGACATGC AACAGTCTCC CCTTTATTCC
    CCTCAGAACA ACATGCCTGG AATCCAGGGA GCCACATCTT CACCTCAACC ACAGGCTACT
    TTATTTCACA ACACTACAGG AGGTACAATG AACCAGCTAC AGAATTCTCC TGGCTCTTCT
    CAACAGACAT CTGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACT
    TCTGGACCAG CTACATTGCC AGATCAGTTG ATGGCCATAA GTCCACCAGG CCAGCCACAA
    AACGAGGGCC AACCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA
    ATGGCATCCT CTATAAACAC CAACCAGAAC ATTGAAAAGA TTGATTTGCT TGTTTCATTG
    CAAAACCAAG GGAACAACTT AACTGGCTCC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812996.1
    CDS834..5189
    Translation

    Target ORF information:

    RefSeq Version XM_010814694.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814694.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
    GTCACCGACA GCAAGGCTAT GCAAGTCGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC
    CGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG
    AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG
    ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG
    GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG
    GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT
    TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG
    CTTTCTCAGC TTACCACGGA CAACAAAGGC AGCTCAAAAG CAGGAAATGG AACATTGGAA
    AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTAT GTGGACAGTA TCCTGTTAAA
    AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CCCAGCACAG AGCTCGGTAC
    CTGACTGAGG GTAGTCGTGG CTCAGTAAAG GACAGAACAC AGCAAGGCTT TCCTACAGTG
    AAGCTGGAAG GCCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT
    CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGGGTAA CTGGGCGAAA TACAACTCCC
    TGCAAAGAAG TGGATATCGA AGGCACTACT GTTATAGAAG TTGGCCTTGA TCCAAGCAAC
    AATATGACAC TGGCGGTTGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTTGAA
    GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA
    GTTAATATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT
    TTATGTACTC AACCAGCAGG AGTGCCAGAA ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG
    GTGAAAGGAG AGGAAGAAGT ATTTTTAATT GGCAAGAACT TTCTGAAAGG AACTAAAGTT
    ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG
    GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA TCAACATATA
    ACTTTGCCAG TAGCAGTGGG AATATATGTA GTAACCAATG CTGGAAGATC TCATGATGTT
    CAGCCTTTCA CTTACACTCC AGACCCAGCA GCTGTTGCTT TGAATGTGAA TGTAAAGAAG
    GAAATATCTA GTCCGGCAAG ACCTTGCTCT TTCGAAGAAG CCATGAAAGC AATGAAAACT
    ACTGGATGTA ACTTAGATAA GGTAAATATG CTTCCTAATG CTCTGATCAC TCCGCTCATA
    TCAAGCACTA TGATTAAGAG TGAAGACATT ACTCCAATGG AAGTAACAGC AGAGAAAAGA
    TCGCCTTCTA TTTTTAAGAC TACAAAGACA GTTGGATCAA CTCAACAAAC GTTAGAAAAT
    CTCTCTCACA TAGCAGGAAA TGGGTCTTTT TCATCATCAT CTTCCCACTT AACTTCTGAA
    AATGAAAAGC AACAACAGAT TCAGCCCAAG GCATACAACC CAGAGACCCT GACAACTATC
    CAAACACAGG ACATTTCACA GCCTGGTACT TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG
    CCCAGCAATG ACGCACTACT ACAGCAGGCT ACACAGTTTC AAACAAGAGA AACTCAGTCT
    AGAGAGGTGT TACAGTCAGA TGGTACAGTG GTTAACTTGT CACACCTGAC TGAGACATCA
    CAGCAACAGC AGCAGTCTCC ACTGCAAGAA CAAGCACAGA CTTTACAGCA GCAGATTTCA
    TCAAATATTT TCCCATCACC AAATAGTGTG AGTCAGCAAC TACAAAATAC GATACAACAC
    TTGCAGGCAG GGAGTTTTAC AGGCAGTACT GCCAGTGGCA GCAATGGAAA TGTTGACTTG
    GTCCAACAAG TTTTAGAGGC ACAACAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT
    GGTAACGAGA ATGTTCAAGA ACAGCTTAGT GCAGACATTT TTCAACAAGT CAGTCAAATT
    CAAAATAGCG TAAGCCCTGG AATGTTTTCC TCAACAGAAC CAGCAGTCCA TACCAGACCA
    GATAATTTAA TAGCTGGAAG AGCTGAAAGT GTTCACCCAC AGAATGAAAA CACATTGTCT
    AATCAGCAAC AGCAACAGCA GCAACAGCAA GTGATGGACT CCTCAGCTGC AATGGTGATG
    GAGATGCAAC AGAGTATCTG CCAGGCAGCT GCCCAGATTC AGTCAGAGCT GTTTCCTTCA
    TCTGCTTCAG CAAATGGAAA CCTTCAGCAG TCTCCAGTTT ACCAGCAGAC TTCTCATATG
    ATGAGCGCAT TATCAGCCAA TGAGGACATG CAAATGCAAT GTGAATTGTT TTCTTCTCCT
    CCGGCAGTTT CTGGAAATGA AACTACTACA ACTACCACAC AGCAGGTTGC AACTTCTGGC
    ACTACCCTGT TTCAGACATC AAATTCAGGA GATGGAGAAG AGACTGGAGC ACAAGCAAAA
    CAGATTCAGA ACAGTGTCTT TCAGACCATG GTCCAAATGC AACATAGTGG GGACAGTCAG
    CCTCAAGTTG GCCTTTTTTC ATCTACAAAA AGTATGATAA GTGTTCAAAA TAGTGGTACC
    CAGCAGCAAG GCAATGGTTT ATTCCAGCAA GGTAATGAGA TGATGTCACT TCAATCAGGG
    AATTTTTTGC AGCAGTCTTC TCATTCACAG GCTCAGCTTT TTCATCCTCA GAATCCTATT
    GCTGATCCTC AGAACCTTTC CCAGGAAACT CAAGGTTCTA TTTTTCATAG TCCAAGTCCT
    ATTGTCCACA GTCAGACTTC TACAGCCTCC TCTGAACAAA TGCAGCCTCC AATGTTTCAC
    TCTCAAAATA CCATGGCTGT GCTGCAGGGC TCTTCTGTTC CTCAAGACCA ACAGTCAGCC
    AACATATTTC TTTCCCAAAG TCCAATGAAT AATCTCCAAA CCAACACAGT GGCCCAGGAA
    GAACAGATTT CATTTTTTGC AGCTCAGAAT TCAATTTCTC CACTTCAGTC AACATCAAAC
    ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG GCTCCAATAT CACACATTCA GACCCCTATG
    CTTTCCCAGG AACAGGCACA ACCCTCCCAG CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG
    GGCTCCCTTC CTCCTAACCC AATGCCTCAA AACCAACAAG GAACAATCTT TCAGTCACAG
    CACTCAATAG TTGCCATCCA GAGTAACTCT CCATCCCAGG AGCAGCAGCA GCAGCAACAA
    CAGCAGCAGC AACAACAGAG CATTTTATTC AGTAACCAGA ACGCCATGGC ACCAATGGCG
    TCTCAAAAGC AGCCACCACC AAACATGATA TTCAATCCAA GTCAAAATCC AGTGGCTAAT
    CAGGAGCAGC AGAACCAATC AATTTTCCAT CAACAAAATA ATATGGCCCC AATGAATCAA
    GAGCAGCAGC CCATGCAATT TCAGAACCAG ACCACGGTTT CCTCACTTCA GAACCCAGGT
    CCTGCCCAAT CCGAATCATC ACAGACCTCC TTGTTCCATA GCTCACCTCA GATTCAGTTG
    GTCCAAGGGT CACCCAGTTC TCAAGAGCAG CAAGTAACAC TCTTCCTCTC TCCAGCATCC
    ATGTCTGCAT TGCAGACCAG TATGAACCAA CAAGACATGC AACAGTCTCC CCTTTATTCC
    CCTCAGAACA ACATGCCTGG AATCCAGGGA GCCACATCTT CACCTCAACC ACAGGCTACT
    TTATTTCACA ACACTACAGG AGGTACAATG AACCAGCTAC AGAATTCTCC TGGCTCTTCT
    CAACAGACAT CTGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACT
    TCTGGACCAG CTACATTGCC AGATCAGTTG ATGGCCATAA GTCCACCAGG CCAGCCACAA
    AACGAGGGCC AACCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA
    ATGGCATCCT CTATAAACAC CAACCAGAAC ATTGAAAAGA TTGATTTGCT TGTTTCATTG
    CAAAACCAAG GGAACAACTT AACTGGCTCC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28567
    Clone ID Related Accession (Same CDS sequence) XM_010814694.2 , XM_010823632.2 , XM_005218872.3 , XM_003583392.4
    Accession Version XM_010823632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
    GTCACCGACA GCAAGGCTAT GCAAGTCGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC
    CGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG
    AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG
    ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG
    GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG
    GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT
    TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG
    CTTTCTCAGC TTACCACGGA CAACAAAGGC AGCTCAAAAG CAGGAAATGG AACATTGGAA
    AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTAT GTGGACAGTA TCCTGTTAAA
    AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CCCAGCACAG AGCTCGGTAC
    CTGACTGAGG GTAGTCGTGG CTCAGTAAAG GACAGAACAC AGCAAGGCTT TCCTACAGTG
    AAGCTGGAAG GCCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT
    CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGGGTAA CTGGGCGAAA TACAACTCCC
    TGCAAAGAAG TGGATATCGA AGGCACTACT GTTATAGAAG TTGGCCTTGA TCCAAGCAAC
    AATATGACAC TGGCGGTTGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTTGAA
    GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA
    GTTAATATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT
    TTATGTACTC AACCAGCAGG AGTGCCAGAA ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG
    GTGAAAGGAG AGGAAGAAGT ATTTTTAATT GGCAAGAACT TTCTGAAAGG AACTAAAGTT
    ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG
    GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA TCAACATATA
    ACTTTGCCAG TAGCAGTGGG AATATATGTA GTAACCAATG CTGGAAGATC TCATGATGTT
    CAGCCTTTCA CTTACACTCC AGACCCAGCA GCTGTTGCTT TGAATGTGAA TGTAAAGAAG
    GAAATATCTA GTCCGGCAAG ACCTTGCTCT TTCGAAGAAG CCATGAAAGC AATGAAAACT
    ACTGGATGTA ACTTAGATAA GGTAAATATG CTTCCTAATG CTCTGATCAC TCCGCTCATA
    TCAAGCACTA TGATTAAGAG TGAAGACATT ACTCCAATGG AAGTAACAGC AGAGAAAAGA
    TCGCCTTCTA TTTTTAAGAC TACAAAGACA GTTGGATCAA CTCAACAAAC GTTAGAAAAT
    CTCTCTCACA TAGCAGGAAA TGGGTCTTTT TCATCATCAT CTTCCCACTT AACTTCTGAA
    AATGAAAAGC AACAACAGAT TCAGCCCAAG GCATACAACC CAGAGACCCT GACAACTATC
    CAAACACAGG ACATTTCACA GCCTGGTACT TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG
    CCCAGCAATG ACGCACTACT ACAGCAGGCT ACACAGTTTC AAACAAGAGA AACTCAGTCT
    AGAGAGGTGT TACAGTCAGA TGGTACAGTG GTTAACTTGT CACACCTGAC TGAGACATCA
    CAGCAACAGC AGCAGTCTCC ACTGCAAGAA CAAGCACAGA CTTTACAGCA GCAGATTTCA
    TCAAATATTT TCCCATCACC AAATAGTGTG AGTCAGCAAC TACAAAATAC GATACAACAC
    TTGCAGGCAG GGAGTTTTAC AGGCAGTACT GCCAGTGGCA GCAATGGAAA TGTTGACTTG
    GTCCAACAAG TTTTAGAGGC ACAACAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT
    GGTAACGAGA ATGTTCAAGA ACAGCTTAGT GCAGACATTT TTCAACAAGT CAGTCAAATT
    CAAAATAGCG TAAGCCCTGG AATGTTTTCC TCAACAGAAC CAGCAGTCCA TACCAGACCA
    GATAATTTAA TAGCTGGAAG AGCTGAAAGT GTTCACCCAC AGAATGAAAA CACATTGTCT
    AATCAGCAAC AGCAACAGCA GCAACAGCAA GTGATGGACT CCTCAGCTGC AATGGTGATG
    GAGATGCAAC AGAGTATCTG CCAGGCAGCT GCCCAGATTC AGTCAGAGCT GTTTCCTTCA
    TCTGCTTCAG CAAATGGAAA CCTTCAGCAG TCTCCAGTTT ACCAGCAGAC TTCTCATATG
    ATGAGCGCAT TATCAGCCAA TGAGGACATG CAAATGCAAT GTGAATTGTT TTCTTCTCCT
    CCGGCAGTTT CTGGAAATGA AACTACTACA ACTACCACAC AGCAGGTTGC AACTTCTGGC
    ACTACCCTGT TTCAGACATC AAATTCAGGA GATGGAGAAG AGACTGGAGC ACAAGCAAAA
    CAGATTCAGA ACAGTGTCTT TCAGACCATG GTCCAAATGC AACATAGTGG GGACAGTCAG
    CCTCAAGTTG GCCTTTTTTC ATCTACAAAA AGTATGATAA GTGTTCAAAA TAGTGGTACC
    CAGCAGCAAG GCAATGGTTT ATTCCAGCAA GGTAATGAGA TGATGTCACT TCAATCAGGG
    AATTTTTTGC AGCAGTCTTC TCATTCACAG GCTCAGCTTT TTCATCCTCA GAATCCTATT
    GCTGATCCTC AGAACCTTTC CCAGGAAACT CAAGGTTCTA TTTTTCATAG TCCAAGTCCT
    ATTGTCCACA GTCAGACTTC TACAGCCTCC TCTGAACAAA TGCAGCCTCC AATGTTTCAC
    TCTCAAAATA CCATGGCTGT GCTGCAGGGC TCTTCTGTTC CTCAAGACCA ACAGTCAGCC
    AACATATTTC TTTCCCAAAG TCCAATGAAT AATCTCCAAA CCAACACAGT GGCCCAGGAA
    GAACAGATTT CATTTTTTGC AGCTCAGAAT TCAATTTCTC CACTTCAGTC AACATCAAAC
    ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG GCTCCAATAT CACACATTCA GACCCCTATG
    CTTTCCCAGG AACAGGCACA ACCCTCCCAG CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG
    GGCTCCCTTC CTCCTAACCC AATGCCTCAA AACCAACAAG GAACAATCTT TCAGTCACAG
    CACTCAATAG TTGCCATCCA GAGTAACTCT CCATCCCAGG AGCAGCAGCA GCAGCAACAA
    CAGCAGCAGC AACAACAGAG CATTTTATTC AGTAACCAGA ACGCCATGGC ACCAATGGCG
    TCTCAAAAGC AGCCACCACC AAACATGATA TTCAATCCAA GTCAAAATCC AGTGGCTAAT
    CAGGAGCAGC AGAACCAATC AATTTTCCAT CAACAAAATA ATATGGCCCC AATGAATCAA
    GAGCAGCAGC CCATGCAATT TCAGAACCAG ACCACGGTTT CCTCACTTCA GAACCCAGGT
    CCTGCCCAAT CCGAATCATC ACAGACCTCC TTGTTCCATA GCTCACCTCA GATTCAGTTG
    GTCCAAGGGT CACCCAGTTC TCAAGAGCAG CAAGTAACAC TCTTCCTCTC TCCAGCATCC
    ATGTCTGCAT TGCAGACCAG TATGAACCAA CAAGACATGC AACAGTCTCC CCTTTATTCC
    CCTCAGAACA ACATGCCTGG AATCCAGGGA GCCACATCTT CACCTCAACC ACAGGCTACT
    TTATTTCACA ACACTACAGG AGGTACAATG AACCAGCTAC AGAATTCTCC TGGCTCTTCT
    CAACAGACAT CTGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACT
    TCTGGACCAG CTACATTGCC AGATCAGTTG ATGGCCATAA GTCCACCAGG CCAGCCACAA
    AACGAGGGCC AACCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA
    ATGGCATCCT CTATAAACAC CAACCAGAAC ATTGAAAAGA TTGATTTGCT TGTTTCATTG
    CAAAACCAAG GGAACAACTT AACTGGCTCC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821934.2
    CDS834..5189
    Translation

    Target ORF information:

    RefSeq Version XM_010823632.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
    GTCACCGACA GCAAGGCTAT GCAAGTCGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC
    CGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG
    AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG
    ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG
    GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG
    GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT
    TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG
    CTTTCTCAGC TTACCACGGA CAACAAAGGC AGCTCAAAAG CAGGAAATGG AACATTGGAA
    AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTAT GTGGACAGTA TCCTGTTAAA
    AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CCCAGCACAG AGCTCGGTAC
    CTGACTGAGG GTAGTCGTGG CTCAGTAAAG GACAGAACAC AGCAAGGCTT TCCTACAGTG
    AAGCTGGAAG GCCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT
    CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGGGTAA CTGGGCGAAA TACAACTCCC
    TGCAAAGAAG TGGATATCGA AGGCACTACT GTTATAGAAG TTGGCCTTGA TCCAAGCAAC
    AATATGACAC TGGCGGTTGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTTGAA
    GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA
    GTTAATATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT
    TTATGTACTC AACCAGCAGG AGTGCCAGAA ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG
    GTGAAAGGAG AGGAAGAAGT ATTTTTAATT GGCAAGAACT TTCTGAAAGG AACTAAAGTT
    ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG
    GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA TCAACATATA
    ACTTTGCCAG TAGCAGTGGG AATATATGTA GTAACCAATG CTGGAAGATC TCATGATGTT
    CAGCCTTTCA CTTACACTCC AGACCCAGCA GCTGTTGCTT TGAATGTGAA TGTAAAGAAG
    GAAATATCTA GTCCGGCAAG ACCTTGCTCT TTCGAAGAAG CCATGAAAGC AATGAAAACT
    ACTGGATGTA ACTTAGATAA GGTAAATATG CTTCCTAATG CTCTGATCAC TCCGCTCATA
    TCAAGCACTA TGATTAAGAG TGAAGACATT ACTCCAATGG AAGTAACAGC AGAGAAAAGA
    TCGCCTTCTA TTTTTAAGAC TACAAAGACA GTTGGATCAA CTCAACAAAC GTTAGAAAAT
    CTCTCTCACA TAGCAGGAAA TGGGTCTTTT TCATCATCAT CTTCCCACTT AACTTCTGAA
    AATGAAAAGC AACAACAGAT TCAGCCCAAG GCATACAACC CAGAGACCCT GACAACTATC
    CAAACACAGG ACATTTCACA GCCTGGTACT TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG
    CCCAGCAATG ACGCACTACT ACAGCAGGCT ACACAGTTTC AAACAAGAGA AACTCAGTCT
    AGAGAGGTGT TACAGTCAGA TGGTACAGTG GTTAACTTGT CACACCTGAC TGAGACATCA
    CAGCAACAGC AGCAGTCTCC ACTGCAAGAA CAAGCACAGA CTTTACAGCA GCAGATTTCA
    TCAAATATTT TCCCATCACC AAATAGTGTG AGTCAGCAAC TACAAAATAC GATACAACAC
    TTGCAGGCAG GGAGTTTTAC AGGCAGTACT GCCAGTGGCA GCAATGGAAA TGTTGACTTG
    GTCCAACAAG TTTTAGAGGC ACAACAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT
    GGTAACGAGA ATGTTCAAGA ACAGCTTAGT GCAGACATTT TTCAACAAGT CAGTCAAATT
    CAAAATAGCG TAAGCCCTGG AATGTTTTCC TCAACAGAAC CAGCAGTCCA TACCAGACCA
    GATAATTTAA TAGCTGGAAG AGCTGAAAGT GTTCACCCAC AGAATGAAAA CACATTGTCT
    AATCAGCAAC AGCAACAGCA GCAACAGCAA GTGATGGACT CCTCAGCTGC AATGGTGATG
    GAGATGCAAC AGAGTATCTG CCAGGCAGCT GCCCAGATTC AGTCAGAGCT GTTTCCTTCA
    TCTGCTTCAG CAAATGGAAA CCTTCAGCAG TCTCCAGTTT ACCAGCAGAC TTCTCATATG
    ATGAGCGCAT TATCAGCCAA TGAGGACATG CAAATGCAAT GTGAATTGTT TTCTTCTCCT
    CCGGCAGTTT CTGGAAATGA AACTACTACA ACTACCACAC AGCAGGTTGC AACTTCTGGC
    ACTACCCTGT TTCAGACATC AAATTCAGGA GATGGAGAAG AGACTGGAGC ACAAGCAAAA
    CAGATTCAGA ACAGTGTCTT TCAGACCATG GTCCAAATGC AACATAGTGG GGACAGTCAG
    CCTCAAGTTG GCCTTTTTTC ATCTACAAAA AGTATGATAA GTGTTCAAAA TAGTGGTACC
    CAGCAGCAAG GCAATGGTTT ATTCCAGCAA GGTAATGAGA TGATGTCACT TCAATCAGGG
    AATTTTTTGC AGCAGTCTTC TCATTCACAG GCTCAGCTTT TTCATCCTCA GAATCCTATT
    GCTGATCCTC AGAACCTTTC CCAGGAAACT CAAGGTTCTA TTTTTCATAG TCCAAGTCCT
    ATTGTCCACA GTCAGACTTC TACAGCCTCC TCTGAACAAA TGCAGCCTCC AATGTTTCAC
    TCTCAAAATA CCATGGCTGT GCTGCAGGGC TCTTCTGTTC CTCAAGACCA ACAGTCAGCC
    AACATATTTC TTTCCCAAAG TCCAATGAAT AATCTCCAAA CCAACACAGT GGCCCAGGAA
    GAACAGATTT CATTTTTTGC AGCTCAGAAT TCAATTTCTC CACTTCAGTC AACATCAAAC
    ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG GCTCCAATAT CACACATTCA GACCCCTATG
    CTTTCCCAGG AACAGGCACA ACCCTCCCAG CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG
    GGCTCCCTTC CTCCTAACCC AATGCCTCAA AACCAACAAG GAACAATCTT TCAGTCACAG
    CACTCAATAG TTGCCATCCA GAGTAACTCT CCATCCCAGG AGCAGCAGCA GCAGCAACAA
    CAGCAGCAGC AACAACAGAG CATTTTATTC AGTAACCAGA ACGCCATGGC ACCAATGGCG
    TCTCAAAAGC AGCCACCACC AAACATGATA TTCAATCCAA GTCAAAATCC AGTGGCTAAT
    CAGGAGCAGC AGAACCAATC AATTTTCCAT CAACAAAATA ATATGGCCCC AATGAATCAA
    GAGCAGCAGC CCATGCAATT TCAGAACCAG ACCACGGTTT CCTCACTTCA GAACCCAGGT
    CCTGCCCAAT CCGAATCATC ACAGACCTCC TTGTTCCATA GCTCACCTCA GATTCAGTTG
    GTCCAAGGGT CACCCAGTTC TCAAGAGCAG CAAGTAACAC TCTTCCTCTC TCCAGCATCC
    ATGTCTGCAT TGCAGACCAG TATGAACCAA CAAGACATGC AACAGTCTCC CCTTTATTCC
    CCTCAGAACA ACATGCCTGG AATCCAGGGA GCCACATCTT CACCTCAACC ACAGGCTACT
    TTATTTCACA ACACTACAGG AGGTACAATG AACCAGCTAC AGAATTCTCC TGGCTCTTCT
    CAACAGACAT CTGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACT
    TCTGGACCAG CTACATTGCC AGATCAGTTG ATGGCCATAA GTCCACCAGG CCAGCCACAA
    AACGAGGGCC AACCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA
    ATGGCATCCT CTATAAACAC CAACCAGAAC ATTGAAAAGA TTGATTTGCT TGTTTCATTG
    CAAAACCAAG GGAACAACTT AACTGGCTCC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28567
    Clone ID Related Accession (Same CDS sequence) XM_010814694.2 , XM_010823632.2 , XM_005218872.3 , XM_003583392.4
    Accession Version XM_005218872.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218872.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
    GTCACCGACA GCAAGGCTAT GCAAGTCGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC
    CGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG
    AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG
    ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG
    GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG
    GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT
    TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG
    CTTTCTCAGC TTACCACGGA CAACAAAGGC AGCTCAAAAG CAGGAAATGG AACATTGGAA
    AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTAT GTGGACAGTA TCCTGTTAAA
    AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CCCAGCACAG AGCTCGGTAC
    CTGACTGAGG GTAGTCGTGG CTCAGTAAAG GACAGAACAC AGCAAGGCTT TCCTACAGTG
    AAGCTGGAAG GCCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT
    CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGGGTAA CTGGGCGAAA TACAACTCCC
    TGCAAAGAAG TGGATATCGA AGGCACTACT GTTATAGAAG TTGGCCTTGA TCCAAGCAAC
    AATATGACAC TGGCGGTTGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTTGAA
    GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA
    GTTAATATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT
    TTATGTACTC AACCAGCAGG AGTGCCAGAA ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG
    GTGAAAGGAG AGGAAGAAGT ATTTTTAATT GGCAAGAACT TTCTGAAAGG AACTAAAGTT
    ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG
    GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA TCAACATATA
    ACTTTGCCAG TAGCAGTGGG AATATATGTA GTAACCAATG CTGGAAGATC TCATGATGTT
    CAGCCTTTCA CTTACACTCC AGACCCAGCA GCTGTTGCTT TGAATGTGAA TGTAAAGAAG
    GAAATATCTA GTCCGGCAAG ACCTTGCTCT TTCGAAGAAG CCATGAAAGC AATGAAAACT
    ACTGGATGTA ACTTAGATAA GGTAAATATG CTTCCTAATG CTCTGATCAC TCCGCTCATA
    TCAAGCACTA TGATTAAGAG TGAAGACATT ACTCCAATGG AAGTAACAGC AGAGAAAAGA
    TCGCCTTCTA TTTTTAAGAC TACAAAGACA GTTGGATCAA CTCAACAAAC GTTAGAAAAT
    CTCTCTCACA TAGCAGGAAA TGGGTCTTTT TCATCATCAT CTTCCCACTT AACTTCTGAA
    AATGAAAAGC AACAACAGAT TCAGCCCAAG GCATACAACC CAGAGACCCT GACAACTATC
    CAAACACAGG ACATTTCACA GCCTGGTACT TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG
    CCCAGCAATG ACGCACTACT ACAGCAGGCT ACACAGTTTC AAACAAGAGA AACTCAGTCT
    AGAGAGGTGT TACAGTCAGA TGGTACAGTG GTTAACTTGT CACACCTGAC TGAGACATCA
    CAGCAACAGC AGCAGTCTCC ACTGCAAGAA CAAGCACAGA CTTTACAGCA GCAGATTTCA
    TCAAATATTT TCCCATCACC AAATAGTGTG AGTCAGCAAC TACAAAATAC GATACAACAC
    TTGCAGGCAG GGAGTTTTAC AGGCAGTACT GCCAGTGGCA GCAATGGAAA TGTTGACTTG
    GTCCAACAAG TTTTAGAGGC ACAACAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT
    GGTAACGAGA ATGTTCAAGA ACAGCTTAGT GCAGACATTT TTCAACAAGT CAGTCAAATT
    CAAAATAGCG TAAGCCCTGG AATGTTTTCC TCAACAGAAC CAGCAGTCCA TACCAGACCA
    GATAATTTAA TAGCTGGAAG AGCTGAAAGT GTTCACCCAC AGAATGAAAA CACATTGTCT
    AATCAGCAAC AGCAACAGCA GCAACAGCAA GTGATGGACT CCTCAGCTGC AATGGTGATG
    GAGATGCAAC AGAGTATCTG CCAGGCAGCT GCCCAGATTC AGTCAGAGCT GTTTCCTTCA
    TCTGCTTCAG CAAATGGAAA CCTTCAGCAG TCTCCAGTTT ACCAGCAGAC TTCTCATATG
    ATGAGCGCAT TATCAGCCAA TGAGGACATG CAAATGCAAT GTGAATTGTT TTCTTCTCCT
    CCGGCAGTTT CTGGAAATGA AACTACTACA ACTACCACAC AGCAGGTTGC AACTTCTGGC
    ACTACCCTGT TTCAGACATC AAATTCAGGA GATGGAGAAG AGACTGGAGC ACAAGCAAAA
    CAGATTCAGA ACAGTGTCTT TCAGACCATG GTCCAAATGC AACATAGTGG GGACAGTCAG
    CCTCAAGTTG GCCTTTTTTC ATCTACAAAA AGTATGATAA GTGTTCAAAA TAGTGGTACC
    CAGCAGCAAG GCAATGGTTT ATTCCAGCAA GGTAATGAGA TGATGTCACT TCAATCAGGG
    AATTTTTTGC AGCAGTCTTC TCATTCACAG GCTCAGCTTT TTCATCCTCA GAATCCTATT
    GCTGATCCTC AGAACCTTTC CCAGGAAACT CAAGGTTCTA TTTTTCATAG TCCAAGTCCT
    ATTGTCCACA GTCAGACTTC TACAGCCTCC TCTGAACAAA TGCAGCCTCC AATGTTTCAC
    TCTCAAAATA CCATGGCTGT GCTGCAGGGC TCTTCTGTTC CTCAAGACCA ACAGTCAGCC
    AACATATTTC TTTCCCAAAG TCCAATGAAT AATCTCCAAA CCAACACAGT GGCCCAGGAA
    GAACAGATTT CATTTTTTGC AGCTCAGAAT TCAATTTCTC CACTTCAGTC AACATCAAAC
    ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG GCTCCAATAT CACACATTCA GACCCCTATG
    CTTTCCCAGG AACAGGCACA ACCCTCCCAG CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG
    GGCTCCCTTC CTCCTAACCC AATGCCTCAA AACCAACAAG GAACAATCTT TCAGTCACAG
    CACTCAATAG TTGCCATCCA GAGTAACTCT CCATCCCAGG AGCAGCAGCA GCAGCAACAA
    CAGCAGCAGC AACAACAGAG CATTTTATTC AGTAACCAGA ACGCCATGGC ACCAATGGCG
    TCTCAAAAGC AGCCACCACC AAACATGATA TTCAATCCAA GTCAAAATCC AGTGGCTAAT
    CAGGAGCAGC AGAACCAATC AATTTTCCAT CAACAAAATA ATATGGCCCC AATGAATCAA
    GAGCAGCAGC CCATGCAATT TCAGAACCAG ACCACGGTTT CCTCACTTCA GAACCCAGGT
    CCTGCCCAAT CCGAATCATC ACAGACCTCC TTGTTCCATA GCTCACCTCA GATTCAGTTG
    GTCCAAGGGT CACCCAGTTC TCAAGAGCAG CAAGTAACAC TCTTCCTCTC TCCAGCATCC
    ATGTCTGCAT TGCAGACCAG TATGAACCAA CAAGACATGC AACAGTCTCC CCTTTATTCC
    CCTCAGAACA ACATGCCTGG AATCCAGGGA GCCACATCTT CACCTCAACC ACAGGCTACT
    TTATTTCACA ACACTACAGG AGGTACAATG AACCAGCTAC AGAATTCTCC TGGCTCTTCT
    CAACAGACAT CTGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACT
    TCTGGACCAG CTACATTGCC AGATCAGTTG ATGGCCATAA GTCCACCAGG CCAGCCACAA
    AACGAGGGCC AACCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA
    ATGGCATCCT CTATAAACAC CAACCAGAAC ATTGAAAAGA TTGATTTGCT TGTTTCATTG
    CAAAACCAAG GGAACAACTT AACTGGCTCC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218929.1
    CDS199..4554
    Translation

    Target ORF information:

    RefSeq Version XM_005218872.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218872.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
    GTCACCGACA GCAAGGCTAT GCAAGTCGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC
    CGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG
    AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG
    ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG
    GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG
    GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT
    TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG
    CTTTCTCAGC TTACCACGGA CAACAAAGGC AGCTCAAAAG CAGGAAATGG AACATTGGAA
    AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTAT GTGGACAGTA TCCTGTTAAA
    AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CCCAGCACAG AGCTCGGTAC
    CTGACTGAGG GTAGTCGTGG CTCAGTAAAG GACAGAACAC AGCAAGGCTT TCCTACAGTG
    AAGCTGGAAG GCCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT
    CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGGGTAA CTGGGCGAAA TACAACTCCC
    TGCAAAGAAG TGGATATCGA AGGCACTACT GTTATAGAAG TTGGCCTTGA TCCAAGCAAC
    AATATGACAC TGGCGGTTGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTTGAA
    GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA
    GTTAATATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT
    TTATGTACTC AACCAGCAGG AGTGCCAGAA ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG
    GTGAAAGGAG AGGAAGAAGT ATTTTTAATT GGCAAGAACT TTCTGAAAGG AACTAAAGTT
    ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG
    GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA TCAACATATA
    ACTTTGCCAG TAGCAGTGGG AATATATGTA GTAACCAATG CTGGAAGATC TCATGATGTT
    CAGCCTTTCA CTTACACTCC AGACCCAGCA GCTGTTGCTT TGAATGTGAA TGTAAAGAAG
    GAAATATCTA GTCCGGCAAG ACCTTGCTCT TTCGAAGAAG CCATGAAAGC AATGAAAACT
    ACTGGATGTA ACTTAGATAA GGTAAATATG CTTCCTAATG CTCTGATCAC TCCGCTCATA
    TCAAGCACTA TGATTAAGAG TGAAGACATT ACTCCAATGG AAGTAACAGC AGAGAAAAGA
    TCGCCTTCTA TTTTTAAGAC TACAAAGACA GTTGGATCAA CTCAACAAAC GTTAGAAAAT
    CTCTCTCACA TAGCAGGAAA TGGGTCTTTT TCATCATCAT CTTCCCACTT AACTTCTGAA
    AATGAAAAGC AACAACAGAT TCAGCCCAAG GCATACAACC CAGAGACCCT GACAACTATC
    CAAACACAGG ACATTTCACA GCCTGGTACT TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG
    CCCAGCAATG ACGCACTACT ACAGCAGGCT ACACAGTTTC AAACAAGAGA AACTCAGTCT
    AGAGAGGTGT TACAGTCAGA TGGTACAGTG GTTAACTTGT CACACCTGAC TGAGACATCA
    CAGCAACAGC AGCAGTCTCC ACTGCAAGAA CAAGCACAGA CTTTACAGCA GCAGATTTCA
    TCAAATATTT TCCCATCACC AAATAGTGTG AGTCAGCAAC TACAAAATAC GATACAACAC
    TTGCAGGCAG GGAGTTTTAC AGGCAGTACT GCCAGTGGCA GCAATGGAAA TGTTGACTTG
    GTCCAACAAG TTTTAGAGGC ACAACAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT
    GGTAACGAGA ATGTTCAAGA ACAGCTTAGT GCAGACATTT TTCAACAAGT CAGTCAAATT
    CAAAATAGCG TAAGCCCTGG AATGTTTTCC TCAACAGAAC CAGCAGTCCA TACCAGACCA
    GATAATTTAA TAGCTGGAAG AGCTGAAAGT GTTCACCCAC AGAATGAAAA CACATTGTCT
    AATCAGCAAC AGCAACAGCA GCAACAGCAA GTGATGGACT CCTCAGCTGC AATGGTGATG
    GAGATGCAAC AGAGTATCTG CCAGGCAGCT GCCCAGATTC AGTCAGAGCT GTTTCCTTCA
    TCTGCTTCAG CAAATGGAAA CCTTCAGCAG TCTCCAGTTT ACCAGCAGAC TTCTCATATG
    ATGAGCGCAT TATCAGCCAA TGAGGACATG CAAATGCAAT GTGAATTGTT TTCTTCTCCT
    CCGGCAGTTT CTGGAAATGA AACTACTACA ACTACCACAC AGCAGGTTGC AACTTCTGGC
    ACTACCCTGT TTCAGACATC AAATTCAGGA GATGGAGAAG AGACTGGAGC ACAAGCAAAA
    CAGATTCAGA ACAGTGTCTT TCAGACCATG GTCCAAATGC AACATAGTGG GGACAGTCAG
    CCTCAAGTTG GCCTTTTTTC ATCTACAAAA AGTATGATAA GTGTTCAAAA TAGTGGTACC
    CAGCAGCAAG GCAATGGTTT ATTCCAGCAA GGTAATGAGA TGATGTCACT TCAATCAGGG
    AATTTTTTGC AGCAGTCTTC TCATTCACAG GCTCAGCTTT TTCATCCTCA GAATCCTATT
    GCTGATCCTC AGAACCTTTC CCAGGAAACT CAAGGTTCTA TTTTTCATAG TCCAAGTCCT
    ATTGTCCACA GTCAGACTTC TACAGCCTCC TCTGAACAAA TGCAGCCTCC AATGTTTCAC
    TCTCAAAATA CCATGGCTGT GCTGCAGGGC TCTTCTGTTC CTCAAGACCA ACAGTCAGCC
    AACATATTTC TTTCCCAAAG TCCAATGAAT AATCTCCAAA CCAACACAGT GGCCCAGGAA
    GAACAGATTT CATTTTTTGC AGCTCAGAAT TCAATTTCTC CACTTCAGTC AACATCAAAC
    ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG GCTCCAATAT CACACATTCA GACCCCTATG
    CTTTCCCAGG AACAGGCACA ACCCTCCCAG CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG
    GGCTCCCTTC CTCCTAACCC AATGCCTCAA AACCAACAAG GAACAATCTT TCAGTCACAG
    CACTCAATAG TTGCCATCCA GAGTAACTCT CCATCCCAGG AGCAGCAGCA GCAGCAACAA
    CAGCAGCAGC AACAACAGAG CATTTTATTC AGTAACCAGA ACGCCATGGC ACCAATGGCG
    TCTCAAAAGC AGCCACCACC AAACATGATA TTCAATCCAA GTCAAAATCC AGTGGCTAAT
    CAGGAGCAGC AGAACCAATC AATTTTCCAT CAACAAAATA ATATGGCCCC AATGAATCAA
    GAGCAGCAGC CCATGCAATT TCAGAACCAG ACCACGGTTT CCTCACTTCA GAACCCAGGT
    CCTGCCCAAT CCGAATCATC ACAGACCTCC TTGTTCCATA GCTCACCTCA GATTCAGTTG
    GTCCAAGGGT CACCCAGTTC TCAAGAGCAG CAAGTAACAC TCTTCCTCTC TCCAGCATCC
    ATGTCTGCAT TGCAGACCAG TATGAACCAA CAAGACATGC AACAGTCTCC CCTTTATTCC
    CCTCAGAACA ACATGCCTGG AATCCAGGGA GCCACATCTT CACCTCAACC ACAGGCTACT
    TTATTTCACA ACACTACAGG AGGTACAATG AACCAGCTAC AGAATTCTCC TGGCTCTTCT
    CAACAGACAT CTGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACT
    TCTGGACCAG CTACATTGCC AGATCAGTTG ATGGCCATAA GTCCACCAGG CCAGCCACAA
    AACGAGGGCC AACCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA
    ATGGCATCCT CTATAAACAC CAACCAGAAC ATTGAAAAGA TTGATTTGCT TGTTTCATTG
    CAAAACCAAG GGAACAACTT AACTGGCTCC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28567
    Clone ID Related Accession (Same CDS sequence) XM_010814694.2 , XM_010823632.2 , XM_005218872.3 , XM_003583392.4
    Accession Version XM_003583392.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583392.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
    GTCACCGACA GCAAGGCTAT GCAAGTCGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC
    CGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG
    AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG
    ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG
    GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG
    GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT
    TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG
    CTTTCTCAGC TTACCACGGA CAACAAAGGC AGCTCAAAAG CAGGAAATGG AACATTGGAA
    AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTAT GTGGACAGTA TCCTGTTAAA
    AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CCCAGCACAG AGCTCGGTAC
    CTGACTGAGG GTAGTCGTGG CTCAGTAAAG GACAGAACAC AGCAAGGCTT TCCTACAGTG
    AAGCTGGAAG GCCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT
    CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGGGTAA CTGGGCGAAA TACAACTCCC
    TGCAAAGAAG TGGATATCGA AGGCACTACT GTTATAGAAG TTGGCCTTGA TCCAAGCAAC
    AATATGACAC TGGCGGTTGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTTGAA
    GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA
    GTTAATATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT
    TTATGTACTC AACCAGCAGG AGTGCCAGAA ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG
    GTGAAAGGAG AGGAAGAAGT ATTTTTAATT GGCAAGAACT TTCTGAAAGG AACTAAAGTT
    ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG
    GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA TCAACATATA
    ACTTTGCCAG TAGCAGTGGG AATATATGTA GTAACCAATG CTGGAAGATC TCATGATGTT
    CAGCCTTTCA CTTACACTCC AGACCCAGCA GCTGTTGCTT TGAATGTGAA TGTAAAGAAG
    GAAATATCTA GTCCGGCAAG ACCTTGCTCT TTCGAAGAAG CCATGAAAGC AATGAAAACT
    ACTGGATGTA ACTTAGATAA GGTAAATATG CTTCCTAATG CTCTGATCAC TCCGCTCATA
    TCAAGCACTA TGATTAAGAG TGAAGACATT ACTCCAATGG AAGTAACAGC AGAGAAAAGA
    TCGCCTTCTA TTTTTAAGAC TACAAAGACA GTTGGATCAA CTCAACAAAC GTTAGAAAAT
    CTCTCTCACA TAGCAGGAAA TGGGTCTTTT TCATCATCAT CTTCCCACTT AACTTCTGAA
    AATGAAAAGC AACAACAGAT TCAGCCCAAG GCATACAACC CAGAGACCCT GACAACTATC
    CAAACACAGG ACATTTCACA GCCTGGTACT TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG
    CCCAGCAATG ACGCACTACT ACAGCAGGCT ACACAGTTTC AAACAAGAGA AACTCAGTCT
    AGAGAGGTGT TACAGTCAGA TGGTACAGTG GTTAACTTGT CACACCTGAC TGAGACATCA
    CAGCAACAGC AGCAGTCTCC ACTGCAAGAA CAAGCACAGA CTTTACAGCA GCAGATTTCA
    TCAAATATTT TCCCATCACC AAATAGTGTG AGTCAGCAAC TACAAAATAC GATACAACAC
    TTGCAGGCAG GGAGTTTTAC AGGCAGTACT GCCAGTGGCA GCAATGGAAA TGTTGACTTG
    GTCCAACAAG TTTTAGAGGC ACAACAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT
    GGTAACGAGA ATGTTCAAGA ACAGCTTAGT GCAGACATTT TTCAACAAGT CAGTCAAATT
    CAAAATAGCG TAAGCCCTGG AATGTTTTCC TCAACAGAAC CAGCAGTCCA TACCAGACCA
    GATAATTTAA TAGCTGGAAG AGCTGAAAGT GTTCACCCAC AGAATGAAAA CACATTGTCT
    AATCAGCAAC AGCAACAGCA GCAACAGCAA GTGATGGACT CCTCAGCTGC AATGGTGATG
    GAGATGCAAC AGAGTATCTG CCAGGCAGCT GCCCAGATTC AGTCAGAGCT GTTTCCTTCA
    TCTGCTTCAG CAAATGGAAA CCTTCAGCAG TCTCCAGTTT ACCAGCAGAC TTCTCATATG
    ATGAGCGCAT TATCAGCCAA TGAGGACATG CAAATGCAAT GTGAATTGTT TTCTTCTCCT
    CCGGCAGTTT CTGGAAATGA AACTACTACA ACTACCACAC AGCAGGTTGC AACTTCTGGC
    ACTACCCTGT TTCAGACATC AAATTCAGGA GATGGAGAAG AGACTGGAGC ACAAGCAAAA
    CAGATTCAGA ACAGTGTCTT TCAGACCATG GTCCAAATGC AACATAGTGG GGACAGTCAG
    CCTCAAGTTG GCCTTTTTTC ATCTACAAAA AGTATGATAA GTGTTCAAAA TAGTGGTACC
    CAGCAGCAAG GCAATGGTTT ATTCCAGCAA GGTAATGAGA TGATGTCACT TCAATCAGGG
    AATTTTTTGC AGCAGTCTTC TCATTCACAG GCTCAGCTTT TTCATCCTCA GAATCCTATT
    GCTGATCCTC AGAACCTTTC CCAGGAAACT CAAGGTTCTA TTTTTCATAG TCCAAGTCCT
    ATTGTCCACA GTCAGACTTC TACAGCCTCC TCTGAACAAA TGCAGCCTCC AATGTTTCAC
    TCTCAAAATA CCATGGCTGT GCTGCAGGGC TCTTCTGTTC CTCAAGACCA ACAGTCAGCC
    AACATATTTC TTTCCCAAAG TCCAATGAAT AATCTCCAAA CCAACACAGT GGCCCAGGAA
    GAACAGATTT CATTTTTTGC AGCTCAGAAT TCAATTTCTC CACTTCAGTC AACATCAAAC
    ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG GCTCCAATAT CACACATTCA GACCCCTATG
    CTTTCCCAGG AACAGGCACA ACCCTCCCAG CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG
    GGCTCCCTTC CTCCTAACCC AATGCCTCAA AACCAACAAG GAACAATCTT TCAGTCACAG
    CACTCAATAG TTGCCATCCA GAGTAACTCT CCATCCCAGG AGCAGCAGCA GCAGCAACAA
    CAGCAGCAGC AACAACAGAG CATTTTATTC AGTAACCAGA ACGCCATGGC ACCAATGGCG
    TCTCAAAAGC AGCCACCACC AAACATGATA TTCAATCCAA GTCAAAATCC AGTGGCTAAT
    CAGGAGCAGC AGAACCAATC AATTTTCCAT CAACAAAATA ATATGGCCCC AATGAATCAA
    GAGCAGCAGC CCATGCAATT TCAGAACCAG ACCACGGTTT CCTCACTTCA GAACCCAGGT
    CCTGCCCAAT CCGAATCATC ACAGACCTCC TTGTTCCATA GCTCACCTCA GATTCAGTTG
    GTCCAAGGGT CACCCAGTTC TCAAGAGCAG CAAGTAACAC TCTTCCTCTC TCCAGCATCC
    ATGTCTGCAT TGCAGACCAG TATGAACCAA CAAGACATGC AACAGTCTCC CCTTTATTCC
    CCTCAGAACA ACATGCCTGG AATCCAGGGA GCCACATCTT CACCTCAACC ACAGGCTACT
    TTATTTCACA ACACTACAGG AGGTACAATG AACCAGCTAC AGAATTCTCC TGGCTCTTCT
    CAACAGACAT CTGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACT
    TCTGGACCAG CTACATTGCC AGATCAGTTG ATGGCCATAA GTCCACCAGG CCAGCCACAA
    AACGAGGGCC AACCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA
    ATGGCATCCT CTATAAACAC CAACCAGAAC ATTGAAAAGA TTGATTTGCT TGTTTCATTG
    CAAAACCAAG GGAACAACTT AACTGGCTCC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003583440.2
    CDS199..4554
    Translation

    Target ORF information:

    RefSeq Version XM_003583392.4
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003583392.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGGTG CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA 
    GTCACCGACA GCAAGGCTAT GCAAGTCGAG AGCTGCTCCT CAGCCTTGGG GGTAAGTAAC
    CGAGGGGTAA GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG
    AAGAGGCACA CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG
    ATGTCCTGCC AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG
    GAGGATTCCC CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG
    GTACCTCGTA AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT
    TGTGAAGAAT CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG
    CTTTCTCAGC TTACCACGGA CAACAAAGGC AGCTCAAAAG CAGGAAATGG AACATTGGAA
    AACCAAAAAG GAACTGGAGT AAAGAAGAGC CCTATGTTAT GTGGACAGTA TCCTGTTAAA
    AGTGAGGGAA AGGAGCTGAA GATAGTTGTA CAACCTGAGA CCCAGCACAG AGCTCGGTAC
    CTGACTGAGG GTAGTCGTGG CTCAGTAAAG GACAGAACAC AGCAAGGCTT TCCTACAGTG
    AAGCTGGAAG GCCATAATGA GCCAGTGGTG TTGCAGGTGT TTGTGGGCAA TGACTCTGGT
    CGAGTGAAAC CACATGGATT TTATCAGGCC TGCAGGGTAA CTGGGCGAAA TACAACTCCC
    TGCAAAGAAG TGGATATCGA AGGCACTACT GTTATAGAAG TTGGCCTTGA TCCAAGCAAC
    AATATGACAC TGGCGGTTGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTTGAA
    GCCAGAATAG GAATTGCTGG TTCCAAGAAA AAGAGCACTC GTGCCAGATT GGTTTTTCGA
    GTTAATATCA CAAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT
    TTATGTACTC AACCAGCAGG AGTGCCAGAA ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG
    GTGAAAGGAG AGGAAGAAGT ATTTTTAATT GGCAAGAACT TTCTGAAAGG AACTAAAGTT
    ATTTTCCAAG AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG
    GAATTATTTC ATCAGAATCA TCTTATTGTG AAGGTTCCCC CATATCATGA TCAACATATA
    ACTTTGCCAG TAGCAGTGGG AATATATGTA GTAACCAATG CTGGAAGATC TCATGATGTT
    CAGCCTTTCA CTTACACTCC AGACCCAGCA GCTGTTGCTT TGAATGTGAA TGTAAAGAAG
    GAAATATCTA GTCCGGCAAG ACCTTGCTCT TTCGAAGAAG CCATGAAAGC AATGAAAACT
    ACTGGATGTA ACTTAGATAA GGTAAATATG CTTCCTAATG CTCTGATCAC TCCGCTCATA
    TCAAGCACTA TGATTAAGAG TGAAGACATT ACTCCAATGG AAGTAACAGC AGAGAAAAGA
    TCGCCTTCTA TTTTTAAGAC TACAAAGACA GTTGGATCAA CTCAACAAAC GTTAGAAAAT
    CTCTCTCACA TAGCAGGAAA TGGGTCTTTT TCATCATCAT CTTCCCACTT AACTTCTGAA
    AATGAAAAGC AACAACAGAT TCAGCCCAAG GCATACAACC CAGAGACCCT GACAACTATC
    CAAACACAGG ACATTTCACA GCCTGGTACT TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG
    CCCAGCAATG ACGCACTACT ACAGCAGGCT ACACAGTTTC AAACAAGAGA AACTCAGTCT
    AGAGAGGTGT TACAGTCAGA TGGTACAGTG GTTAACTTGT CACACCTGAC TGAGACATCA
    CAGCAACAGC AGCAGTCTCC ACTGCAAGAA CAAGCACAGA CTTTACAGCA GCAGATTTCA
    TCAAATATTT TCCCATCACC AAATAGTGTG AGTCAGCAAC TACAAAATAC GATACAACAC
    TTGCAGGCAG GGAGTTTTAC AGGCAGTACT GCCAGTGGCA GCAATGGAAA TGTTGACTTG
    GTCCAACAAG TTTTAGAGGC ACAACAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT
    GGTAACGAGA ATGTTCAAGA ACAGCTTAGT GCAGACATTT TTCAACAAGT CAGTCAAATT
    CAAAATAGCG TAAGCCCTGG AATGTTTTCC TCAACAGAAC CAGCAGTCCA TACCAGACCA
    GATAATTTAA TAGCTGGAAG AGCTGAAAGT GTTCACCCAC AGAATGAAAA CACATTGTCT
    AATCAGCAAC AGCAACAGCA GCAACAGCAA GTGATGGACT CCTCAGCTGC AATGGTGATG
    GAGATGCAAC AGAGTATCTG CCAGGCAGCT GCCCAGATTC AGTCAGAGCT GTTTCCTTCA
    TCTGCTTCAG CAAATGGAAA CCTTCAGCAG TCTCCAGTTT ACCAGCAGAC TTCTCATATG
    ATGAGCGCAT TATCAGCCAA TGAGGACATG CAAATGCAAT GTGAATTGTT TTCTTCTCCT
    CCGGCAGTTT CTGGAAATGA AACTACTACA ACTACCACAC AGCAGGTTGC AACTTCTGGC
    ACTACCCTGT TTCAGACATC AAATTCAGGA GATGGAGAAG AGACTGGAGC ACAAGCAAAA
    CAGATTCAGA ACAGTGTCTT TCAGACCATG GTCCAAATGC AACATAGTGG GGACAGTCAG
    CCTCAAGTTG GCCTTTTTTC ATCTACAAAA AGTATGATAA GTGTTCAAAA TAGTGGTACC
    CAGCAGCAAG GCAATGGTTT ATTCCAGCAA GGTAATGAGA TGATGTCACT TCAATCAGGG
    AATTTTTTGC AGCAGTCTTC TCATTCACAG GCTCAGCTTT TTCATCCTCA GAATCCTATT
    GCTGATCCTC AGAACCTTTC CCAGGAAACT CAAGGTTCTA TTTTTCATAG TCCAAGTCCT
    ATTGTCCACA GTCAGACTTC TACAGCCTCC TCTGAACAAA TGCAGCCTCC AATGTTTCAC
    TCTCAAAATA CCATGGCTGT GCTGCAGGGC TCTTCTGTTC CTCAAGACCA ACAGTCAGCC
    AACATATTTC TTTCCCAAAG TCCAATGAAT AATCTCCAAA CCAACACAGT GGCCCAGGAA
    GAACAGATTT CATTTTTTGC AGCTCAGAAT TCAATTTCTC CACTTCAGTC AACATCAAAC
    ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG GCTCCAATAT CACACATTCA GACCCCTATG
    CTTTCCCAGG AACAGGCACA ACCCTCCCAG CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG
    GGCTCCCTTC CTCCTAACCC AATGCCTCAA AACCAACAAG GAACAATCTT TCAGTCACAG
    CACTCAATAG TTGCCATCCA GAGTAACTCT CCATCCCAGG AGCAGCAGCA GCAGCAACAA
    CAGCAGCAGC AACAACAGAG CATTTTATTC AGTAACCAGA ACGCCATGGC ACCAATGGCG
    TCTCAAAAGC AGCCACCACC AAACATGATA TTCAATCCAA GTCAAAATCC AGTGGCTAAT
    CAGGAGCAGC AGAACCAATC AATTTTCCAT CAACAAAATA ATATGGCCCC AATGAATCAA
    GAGCAGCAGC CCATGCAATT TCAGAACCAG ACCACGGTTT CCTCACTTCA GAACCCAGGT
    CCTGCCCAAT CCGAATCATC ACAGACCTCC TTGTTCCATA GCTCACCTCA GATTCAGTTG
    GTCCAAGGGT CACCCAGTTC TCAAGAGCAG CAAGTAACAC TCTTCCTCTC TCCAGCATCC
    ATGTCTGCAT TGCAGACCAG TATGAACCAA CAAGACATGC AACAGTCTCC CCTTTATTCC
    CCTCAGAACA ACATGCCTGG AATCCAGGGA GCCACATCTT CACCTCAACC ACAGGCTACT
    TTATTTCACA ACACTACAGG AGGTACAATG AACCAGCTAC AGAATTCTCC TGGCTCTTCT
    CAACAGACAT CTGGAATGTT CTTATTTGGC ATTCAAAATA ACTGTAGTCA GCTTTTAACT
    TCTGGACCAG CTACATTGCC AGATCAGTTG ATGGCCATAA GTCCACCAGG CCAGCCACAA
    AACGAGGGCC AACCACCTGT GACAACACTT CTTTCTCAGC AAATGCCAGA GAATTCTCCA
    ATGGCATCCT CTATAAACAC CAACCAGAAC ATTGAAAAGA TTGATTTGCT TGTTTCATTG
    CAAAACCAAG GGAACAACTT AACTGGCTCC TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28568
    Clone ID Related Accession (Same CDS sequence) XM_005218873.3 , XM_005218874.3 , XM_010823634.2 , XM_010814695.2 , XM_005195290.3 , XM_005195289.3
    Accession Version XM_005218873.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218873.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218930.1
    CDS170..4135
    Translation

    Target ORF information:

    RefSeq Version XM_005218873.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218873.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28568
    Clone ID Related Accession (Same CDS sequence) XM_005218873.3 , XM_005218874.3 , XM_010823634.2 , XM_010814695.2 , XM_005195290.3 , XM_005195289.3
    Accession Version XM_005218874.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218874.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218931.1
    CDS109..4074
    Translation

    Target ORF information:

    RefSeq Version XM_005218874.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218874.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28568
    Clone ID Related Accession (Same CDS sequence) XM_005218873.3 , XM_005218874.3 , XM_010823634.2 , XM_010814695.2 , XM_005195290.3 , XM_005195289.3
    Accession Version XM_010823634.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821936.2
    CDS804..4769
    Translation

    Target ORF information:

    RefSeq Version XM_010823634.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823634.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28568
    Clone ID Related Accession (Same CDS sequence) XM_005218873.3 , XM_005218874.3 , XM_010823634.2 , XM_010814695.2 , XM_005195290.3 , XM_005195289.3
    Accession Version XM_010814695.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812997.1
    CDS804..4769
    Translation

    Target ORF information:

    RefSeq Version XM_010814695.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814695.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28568
    Clone ID Related Accession (Same CDS sequence) XM_005218873.3 , XM_005218874.3 , XM_010823634.2 , XM_010814695.2 , XM_005195290.3 , XM_005195289.3
    Accession Version XM_005195290.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195290.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195347.2
    CDS109..4074
    Translation

    Target ORF information:

    RefSeq Version XM_005195290.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195290.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28568
    Clone ID Related Accession (Same CDS sequence) XM_005218873.3 , XM_005218874.3 , XM_010823634.2 , XM_010814695.2 , XM_005195290.3 , XM_005195289.3
    Accession Version XM_005195289.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195346.2
    CDS170..4135
    Translation

    Target ORF information:

    RefSeq Version XM_005195289.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195289.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCTTC AGCTCCCTCC TCTTCCTCCA TGGGCGGTGC TTGCAGCTCC TTTACCACCT 
    CTTCCAGCCC TACCATTTAT TCTACCTCAG TCACCGACAG CAAGGCTATG CAAGTCGAGA
    GCTGCTCCTC AGCCTTGGGG AACATTGGAA AACCAAAAAG GAACTGGAGT AAAGAAGAGC
    CCTATGTTAT GTGGACAGTA TCCTGTTAAA AGTGAGGGAA AGGAGCTGAA GATAGTTGTA
    CAACCTGAGA CCCAGCACAG AGCTCGGTAC CTGACTGAGG GTAGTCGTGG CTCAGTAAAG
    GACAGAACAC AGCAAGGCTT TCCTACAGTG AAGCTGGAAG GCCATAATGA GCCAGTGGTG
    TTGCAGGTGT TTGTGGGCAA TGACTCTGGT CGAGTGAAAC CACATGGATT TTATCAGGCC
    TGCAGGGTAA CTGGGCGAAA TACAACTCCC TGCAAAGAAG TGGATATCGA AGGCACTACT
    GTTATAGAAG TTGGCCTTGA TCCAAGCAAC AATATGACAC TGGCGGTTGA CTGCGTAGGG
    ATATTGAAAT TGAGGAATGC TGATGTTGAA GCCAGAATAG GAATTGCTGG TTCCAAGAAA
    AAGAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA CAAGGAAAGA TGGCTCCACT
    TTGACACTGC AAACACCCTC TTCTCCAATT TTATGTACTC AACCAGCAGG AGTGCCAGAA
    ATCTTAAAGA AGAGCTTGCA TAGCTGTTCG GTGAAAGGAG AGGAAGAAGT ATTTTTAATT
    GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG AAAATGTTTC TGATGAAAAC
    TCTTGGAAGT CAGAAGCTGA AATTGATATG GAATTATTTC ATCAGAATCA TCTTATTGTG
    AAGGTTCCCC CATATCATGA TCAACATATA ACTTTGCCAG TAGCAGTGGG AATATATGTA
    GTAACCAATG CTGGAAGATC TCATGATGTT CAGCCTTTCA CTTACACTCC AGACCCAGCA
    GCTGTTGCTT TGAATGTGAA TGTAAAGAAG GAAATATCTA GTCCGGCAAG ACCTTGCTCT
    TTCGAAGAAG CCATGAAAGC AATGAAAACT ACTGGATGTA ACTTAGATAA GGTAAATATG
    CTTCCTAATG CTCTGATCAC TCCGCTCATA TCAAGCACTA TGATTAAGAG TGAAGACATT
    ACTCCAATGG AAGTAACAGC AGAGAAAAGA TCGCCTTCTA TTTTTAAGAC TACAAAGACA
    GTTGGATCAA CTCAACAAAC GTTAGAAAAT CTCTCTCACA TAGCAGGAAA TGGGTCTTTT
    TCATCATCAT CTTCCCACTT AACTTCTGAA AATGAAAAGC AACAACAGAT TCAGCCCAAG
    GCATACAACC CAGAGACCCT GACAACTATC CAAACACAGG ACATTTCACA GCCTGGTACT
    TTTCCAGCGG TTTCTGCGTC TAGTCAGCTG CCCAGCAATG ACGCACTACT ACAGCAGGCT
    ACACAGTTTC AAACAAGAGA AACTCAGTCT AGAGAGGTGT TACAGTCAGA TGGTACAGTG
    GTTAACTTGT CACACCTGAC TGAGACATCA CAGCAACAGC AGCAGTCTCC ACTGCAAGAA
    CAAGCACAGA CTTTACAGCA GCAGATTTCA TCAAATATTT TCCCATCACC AAATAGTGTG
    AGTCAGCAAC TACAAAATAC GATACAACAC TTGCAGGCAG GGAGTTTTAC AGGCAGTACT
    GCCAGTGGCA GCAATGGAAA TGTTGACTTG GTCCAACAAG TTTTAGAGGC ACAACAGCAG
    TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAACGAGA ATGTTCAAGA ACAGCTTAGT
    GCAGACATTT TTCAACAAGT CAGTCAAATT CAAAATAGCG TAAGCCCTGG AATGTTTTCC
    TCAACAGAAC CAGCAGTCCA TACCAGACCA GATAATTTAA TAGCTGGAAG AGCTGAAAGT
    GTTCACCCAC AGAATGAAAA CACATTGTCT AATCAGCAAC AGCAACAGCA GCAACAGCAA
    GTGATGGACT CCTCAGCTGC AATGGTGATG GAGATGCAAC AGAGTATCTG CCAGGCAGCT
    GCCCAGATTC AGTCAGAGCT GTTTCCTTCA TCTGCTTCAG CAAATGGAAA CCTTCAGCAG
    TCTCCAGTTT ACCAGCAGAC TTCTCATATG ATGAGCGCAT TATCAGCCAA TGAGGACATG
    CAAATGCAAT GTGAATTGTT TTCTTCTCCT CCGGCAGTTT CTGGAAATGA AACTACTACA
    ACTACCACAC AGCAGGTTGC AACTTCTGGC ACTACCCTGT TTCAGACATC AAATTCAGGA
    GATGGAGAAG AGACTGGAGC ACAAGCAAAA CAGATTCAGA ACAGTGTCTT TCAGACCATG
    GTCCAAATGC AACATAGTGG GGACAGTCAG CCTCAAGTTG GCCTTTTTTC ATCTACAAAA
    AGTATGATAA GTGTTCAAAA TAGTGGTACC CAGCAGCAAG GCAATGGTTT ATTCCAGCAA
    GGTAATGAGA TGATGTCACT TCAATCAGGG AATTTTTTGC AGCAGTCTTC TCATTCACAG
    GCTCAGCTTT TTCATCCTCA GAATCCTATT GCTGATCCTC AGAACCTTTC CCAGGAAACT
    CAAGGTTCTA TTTTTCATAG TCCAAGTCCT ATTGTCCACA GTCAGACTTC TACAGCCTCC
    TCTGAACAAA TGCAGCCTCC AATGTTTCAC TCTCAAAATA CCATGGCTGT GCTGCAGGGC
    TCTTCTGTTC CTCAAGACCA ACAGTCAGCC AACATATTTC TTTCCCAAAG TCCAATGAAT
    AATCTCCAAA CCAACACAGT GGCCCAGGAA GAACAGATTT CATTTTTTGC AGCTCAGAAT
    TCAATTTCTC CACTTCAGTC AACATCAAAC ACTGAGCAGC AAGCTGCTTT CCAGCAGCAG
    GCTCCAATAT CACACATTCA GACCCCTATG CTTTCCCAGG AACAGGCACA ACCCTCCCAG
    CAAGGTCTGT TTCAGCCTCA GGTTTCCCTG GGCTCCCTTC CTCCTAACCC AATGCCTCAA
    AACCAACAAG GAACAATCTT TCAGTCACAG CACTCAATAG TTGCCATCCA GAGTAACTCT
    CCATCCCAGG AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACAACAGAG CATTTTATTC
    AGTAACCAGA ACGCCATGGC ACCAATGGCG TCTCAAAAGC AGCCACCACC AAACATGATA
    TTCAATCCAA GTCAAAATCC AGTGGCTAAT CAGGAGCAGC AGAACCAATC AATTTTCCAT
    CAACAAAATA ATATGGCCCC AATGAATCAA GAGCAGCAGC CCATGCAATT TCAGAACCAG
    ACCACGGTTT CCTCACTTCA GAACCCAGGT CCTGCCCAAT CCGAATCATC ACAGACCTCC
    TTGTTCCATA GCTCACCTCA GATTCAGTTG GTCCAAGGGT CACCCAGTTC TCAAGAGCAG
    CAAGTAACAC TCTTCCTCTC TCCAGCATCC ATGTCTGCAT TGCAGACCAG TATGAACCAA
    CAAGACATGC AACAGTCTCC CCTTTATTCC CCTCAGAACA ACATGCCTGG AATCCAGGGA
    GCCACATCTT CACCTCAACC ACAGGCTACT TTATTTCACA ACACTACAGG AGGTACAATG
    AACCAGCTAC AGAATTCTCC TGGCTCTTCT CAACAGACAT CTGGAATGTT CTTATTTGGC
    ATTCAAAATA ACTGTAGTCA GCTTTTAACT TCTGGACCAG CTACATTGCC AGATCAGTTG
    ATGGCCATAA GTCCACCAGG CCAGCCACAA AACGAGGGCC AACCACCTGT GACAACACTT
    CTTTCTCAGC AAATGCCAGA GAATTCTCCA ATGGCATCCT CTATAAACAC CAACCAGAAC
    ATTGAAAAGA TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT AACTGGCTCC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53919
    Clone ID Related Accession (Same CDS sequence) XM_015467397.1 , XM_010814696.2
    Accession Version XM_015467397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTATGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA 
    CCTGAGACCC AGCACAGAGC TCGGTACCTG ACTGAGGGTA GTCGTGGCTC AGTAAAGGAC
    AGAACACAGC AAGGCTTTCC TACAGTGAAG CTGGAAGGCC ATAATGAGCC AGTGGTGTTG
    CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC
    AGGGTAACTG GGCGAAATAC AACTCCCTGC AAAGAAGTGG ATATCGAAGG CACTACTGTT
    ATAGAAGTTG GCCTTGATCC AAGCAACAAT ATGACACTGG CGGTTGACTG CGTAGGGATA
    TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAG
    AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACAA GGAAAGATGG CTCCACTTTG
    ACACTGCAAA CACCCTCTTC TCCAATTTTA TGTACTCAAC CAGCAGGAGT GCCAGAAATC
    TTAAAGAAGA GCTTGCATAG CTGTTCGGTG AAAGGAGAGG AAGAAGTATT TTTAATTGGC
    AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT
    TGGAAGTCAG AAGCTGAAAT TGATATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG
    GTTCCCCCAT ATCATGATCA ACATATAACT TTGCCAGTAG CAGTGGGAAT ATATGTAGTA
    ACCAATGCTG GAAGATCTCA TGATGTTCAG CCTTTCACTT ACACTCCAGA CCCAGCAGCT
    GTTGCTTTGA ATGTGAATGT AAAGAAGGAA ATATCTAGTC CGGCAAGACC TTGCTCTTTC
    GAAGAAGCCA TGAAAGCAAT GAAAACTACT GGATGTAACT TAGATAAGGT AAATATGCTT
    CCTAATGCTC TGATCACTCC GCTCATATCA AGCACTATGA TTAAGAGTGA AGACATTACT
    CCAATGGAAG TAACAGCAGA GAAAAGATCG CCTTCTATTT TTAAGACTAC AAAGACAGTT
    GGATCAACTC AACAAACGTT AGAAAATCTC TCTCACATAG CAGGAAATGG GTCTTTTTCA
    TCATCATCTT CCCACTTAAC TTCTGAAAAT GAAAAGCAAC AACAGATTCA GCCCAAGGCA
    TACAACCCAG AGACCCTGAC AACTATCCAA ACACAGGACA TTTCACAGCC TGGTACTTTT
    CCAGCGGTTT CTGCGTCTAG TCAGCTGCCC AGCAATGACG CACTACTACA GCAGGCTACA
    CAGTTTCAAA CAAGAGAAAC TCAGTCTAGA GAGGTGTTAC AGTCAGATGG TACAGTGGTT
    AACTTGTCAC ACCTGACTGA GACATCACAG CAACAGCAGC AGTCTCCACT GCAAGAACAA
    GCACAGACTT TACAGCAGCA GATTTCATCA AATATTTTCC CATCACCAAA TAGTGTGAGT
    CAGCAACTAC AAAATACGAT ACAACACTTG CAGGCAGGGA GTTTTACAGG CAGTACTGCC
    AGTGGCAGCA ATGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAGCAGTTA
    TCTTCAGTTT TATTTTCTGC TCCAGATGGT AACGAGAATG TTCAAGAACA GCTTAGTGCA
    GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGCGTAA GCCCTGGAAT GTTTTCCTCA
    ACAGAACCAG CAGTCCATAC CAGACCAGAT AATTTAATAG CTGGAAGAGC TGAAAGTGTT
    CACCCACAGA ATGAAAACAC ATTGTCTAAT CAGCAACAGC AACAGCAGCA ACAGCAAGTG
    ATGGACTCCT CAGCTGCAAT GGTGATGGAG ATGCAACAGA GTATCTGCCA GGCAGCTGCC
    CAGATTCAGT CAGAGCTGTT TCCTTCATCT GCTTCAGCAA ATGGAAACCT TCAGCAGTCT
    CCAGTTTACC AGCAGACTTC TCATATGATG AGCGCATTAT CAGCCAATGA GGACATGCAA
    ATGCAATGTG AATTGTTTTC TTCTCCTCCG GCAGTTTCTG GAAATGAAAC TACTACAACT
    ACCACACAGC AGGTTGCAAC TTCTGGCACT ACCCTGTTTC AGACATCAAA TTCAGGAGAT
    GGAGAAGAGA CTGGAGCACA AGCAAAACAG ATTCAGAACA GTGTCTTTCA GACCATGGTC
    CAAATGCAAC ATAGTGGGGA CAGTCAGCCT CAAGTTGGCC TTTTTTCATC TACAAAAAGT
    ATGATAAGTG TTCAAAATAG TGGTACCCAG CAGCAAGGCA ATGGTTTATT CCAGCAAGGT
    AATGAGATGA TGTCACTTCA ATCAGGGAAT TTTTTGCAGC AGTCTTCTCA TTCACAGGCT
    CAGCTTTTTC ATCCTCAGAA TCCTATTGCT GATCCTCAGA ACCTTTCCCA GGAAACTCAA
    GGTTCTATTT TTCATAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AGCCTCCTCT
    GAACAAATGC AGCCTCCAAT GTTTCACTCT CAAAATACCA TGGCTGTGCT GCAGGGCTCT
    TCTGTTCCTC AAGACCAACA GTCAGCCAAC ATATTTCTTT CCCAAAGTCC AATGAATAAT
    CTCCAAACCA ACACAGTGGC CCAGGAAGAA CAGATTTCAT TTTTTGCAGC TCAGAATTCA
    ATTTCTCCAC TTCAGTCAAC ATCAAACACT GAGCAGCAAG CTGCTTTCCA GCAGCAGGCT
    CCAATATCAC ACATTCAGAC CCCTATGCTT TCCCAGGAAC AGGCACAACC CTCCCAGCAA
    GGTCTGTTTC AGCCTCAGGT TTCCCTGGGC TCCCTTCCTC CTAACCCAAT GCCTCAAAAC
    CAACAAGGAA CAATCTTTCA GTCACAGCAC TCAATAGTTG CCATCCAGAG TAACTCTCCA
    TCCCAGGAGC AGCAGCAGCA GCAACAACAG CAGCAGCAAC AACAGAGCAT TTTATTCAGT
    AACCAGAACG CCATGGCACC AATGGCGTCT CAAAAGCAGC CACCACCAAA CATGATATTC
    AATCCAAGTC AAAATCCAGT GGCTAATCAG GAGCAGCAGA ACCAATCAAT TTTCCATCAA
    CAAAATAATA TGGCCCCAAT GAATCAAGAG CAGCAGCCCA TGCAATTTCA GAACCAGACC
    ACGGTTTCCT CACTTCAGAA CCCAGGTCCT GCCCAATCCG AATCATCACA GACCTCCTTG
    TTCCATAGCT CACCTCAGAT TCAGTTGGTC CAAGGGTCAC CCAGTTCTCA AGAGCAGCAA
    GTAACACTCT TCCTCTCTCC AGCATCCATG TCTGCATTGC AGACCAGTAT GAACCAACAA
    GACATGCAAC AGTCTCCCCT TTATTCCCCT CAGAACAACA TGCCTGGAAT CCAGGGAGCC
    ACATCTTCAC CTCAACCACA GGCTACTTTA TTTCACAACA CTACAGGAGG TACAATGAAC
    CAGCTACAGA ATTCTCCTGG CTCTTCTCAA CAGACATCTG GAATGTTCTT ATTTGGCATT
    CAAAATAACT GTAGTCAGCT TTTAACTTCT GGACCAGCTA CATTGCCAGA TCAGTTGATG
    GCCATAAGTC CACCAGGCCA GCCACAAAAC GAGGGCCAAC CACCTGTGAC AACACTTCTT
    TCTCAGCAAA TGCCAGAGAA TTCTCCAATG GCATCCTCTA TAAACACCAA CCAGAACATT
    GAAAAGATTG ATTTGCTTGT TTCATTGCAA AACCAAGGGA ACAACTTAAC TGGCTCCTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322883.1
    CDS151..3933
    Translation

    Target ORF information:

    RefSeq Version XM_015467397.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTATGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA 
    CCTGAGACCC AGCACAGAGC TCGGTACCTG ACTGAGGGTA GTCGTGGCTC AGTAAAGGAC
    AGAACACAGC AAGGCTTTCC TACAGTGAAG CTGGAAGGCC ATAATGAGCC AGTGGTGTTG
    CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC
    AGGGTAACTG GGCGAAATAC AACTCCCTGC AAAGAAGTGG ATATCGAAGG CACTACTGTT
    ATAGAAGTTG GCCTTGATCC AAGCAACAAT ATGACACTGG CGGTTGACTG CGTAGGGATA
    TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAG
    AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACAA GGAAAGATGG CTCCACTTTG
    ACACTGCAAA CACCCTCTTC TCCAATTTTA TGTACTCAAC CAGCAGGAGT GCCAGAAATC
    TTAAAGAAGA GCTTGCATAG CTGTTCGGTG AAAGGAGAGG AAGAAGTATT TTTAATTGGC
    AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT
    TGGAAGTCAG AAGCTGAAAT TGATATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG
    GTTCCCCCAT ATCATGATCA ACATATAACT TTGCCAGTAG CAGTGGGAAT ATATGTAGTA
    ACCAATGCTG GAAGATCTCA TGATGTTCAG CCTTTCACTT ACACTCCAGA CCCAGCAGCT
    GTTGCTTTGA ATGTGAATGT AAAGAAGGAA ATATCTAGTC CGGCAAGACC TTGCTCTTTC
    GAAGAAGCCA TGAAAGCAAT GAAAACTACT GGATGTAACT TAGATAAGGT AAATATGCTT
    CCTAATGCTC TGATCACTCC GCTCATATCA AGCACTATGA TTAAGAGTGA AGACATTACT
    CCAATGGAAG TAACAGCAGA GAAAAGATCG CCTTCTATTT TTAAGACTAC AAAGACAGTT
    GGATCAACTC AACAAACGTT AGAAAATCTC TCTCACATAG CAGGAAATGG GTCTTTTTCA
    TCATCATCTT CCCACTTAAC TTCTGAAAAT GAAAAGCAAC AACAGATTCA GCCCAAGGCA
    TACAACCCAG AGACCCTGAC AACTATCCAA ACACAGGACA TTTCACAGCC TGGTACTTTT
    CCAGCGGTTT CTGCGTCTAG TCAGCTGCCC AGCAATGACG CACTACTACA GCAGGCTACA
    CAGTTTCAAA CAAGAGAAAC TCAGTCTAGA GAGGTGTTAC AGTCAGATGG TACAGTGGTT
    AACTTGTCAC ACCTGACTGA GACATCACAG CAACAGCAGC AGTCTCCACT GCAAGAACAA
    GCACAGACTT TACAGCAGCA GATTTCATCA AATATTTTCC CATCACCAAA TAGTGTGAGT
    CAGCAACTAC AAAATACGAT ACAACACTTG CAGGCAGGGA GTTTTACAGG CAGTACTGCC
    AGTGGCAGCA ATGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAGCAGTTA
    TCTTCAGTTT TATTTTCTGC TCCAGATGGT AACGAGAATG TTCAAGAACA GCTTAGTGCA
    GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGCGTAA GCCCTGGAAT GTTTTCCTCA
    ACAGAACCAG CAGTCCATAC CAGACCAGAT AATTTAATAG CTGGAAGAGC TGAAAGTGTT
    CACCCACAGA ATGAAAACAC ATTGTCTAAT CAGCAACAGC AACAGCAGCA ACAGCAAGTG
    ATGGACTCCT CAGCTGCAAT GGTGATGGAG ATGCAACAGA GTATCTGCCA GGCAGCTGCC
    CAGATTCAGT CAGAGCTGTT TCCTTCATCT GCTTCAGCAA ATGGAAACCT TCAGCAGTCT
    CCAGTTTACC AGCAGACTTC TCATATGATG AGCGCATTAT CAGCCAATGA GGACATGCAA
    ATGCAATGTG AATTGTTTTC TTCTCCTCCG GCAGTTTCTG GAAATGAAAC TACTACAACT
    ACCACACAGC AGGTTGCAAC TTCTGGCACT ACCCTGTTTC AGACATCAAA TTCAGGAGAT
    GGAGAAGAGA CTGGAGCACA AGCAAAACAG ATTCAGAACA GTGTCTTTCA GACCATGGTC
    CAAATGCAAC ATAGTGGGGA CAGTCAGCCT CAAGTTGGCC TTTTTTCATC TACAAAAAGT
    ATGATAAGTG TTCAAAATAG TGGTACCCAG CAGCAAGGCA ATGGTTTATT CCAGCAAGGT
    AATGAGATGA TGTCACTTCA ATCAGGGAAT TTTTTGCAGC AGTCTTCTCA TTCACAGGCT
    CAGCTTTTTC ATCCTCAGAA TCCTATTGCT GATCCTCAGA ACCTTTCCCA GGAAACTCAA
    GGTTCTATTT TTCATAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AGCCTCCTCT
    GAACAAATGC AGCCTCCAAT GTTTCACTCT CAAAATACCA TGGCTGTGCT GCAGGGCTCT
    TCTGTTCCTC AAGACCAACA GTCAGCCAAC ATATTTCTTT CCCAAAGTCC AATGAATAAT
    CTCCAAACCA ACACAGTGGC CCAGGAAGAA CAGATTTCAT TTTTTGCAGC TCAGAATTCA
    ATTTCTCCAC TTCAGTCAAC ATCAAACACT GAGCAGCAAG CTGCTTTCCA GCAGCAGGCT
    CCAATATCAC ACATTCAGAC CCCTATGCTT TCCCAGGAAC AGGCACAACC CTCCCAGCAA
    GGTCTGTTTC AGCCTCAGGT TTCCCTGGGC TCCCTTCCTC CTAACCCAAT GCCTCAAAAC
    CAACAAGGAA CAATCTTTCA GTCACAGCAC TCAATAGTTG CCATCCAGAG TAACTCTCCA
    TCCCAGGAGC AGCAGCAGCA GCAACAACAG CAGCAGCAAC AACAGAGCAT TTTATTCAGT
    AACCAGAACG CCATGGCACC AATGGCGTCT CAAAAGCAGC CACCACCAAA CATGATATTC
    AATCCAAGTC AAAATCCAGT GGCTAATCAG GAGCAGCAGA ACCAATCAAT TTTCCATCAA
    CAAAATAATA TGGCCCCAAT GAATCAAGAG CAGCAGCCCA TGCAATTTCA GAACCAGACC
    ACGGTTTCCT CACTTCAGAA CCCAGGTCCT GCCCAATCCG AATCATCACA GACCTCCTTG
    TTCCATAGCT CACCTCAGAT TCAGTTGGTC CAAGGGTCAC CCAGTTCTCA AGAGCAGCAA
    GTAACACTCT TCCTCTCTCC AGCATCCATG TCTGCATTGC AGACCAGTAT GAACCAACAA
    GACATGCAAC AGTCTCCCCT TTATTCCCCT CAGAACAACA TGCCTGGAAT CCAGGGAGCC
    ACATCTTCAC CTCAACCACA GGCTACTTTA TTTCACAACA CTACAGGAGG TACAATGAAC
    CAGCTACAGA ATTCTCCTGG CTCTTCTCAA CAGACATCTG GAATGTTCTT ATTTGGCATT
    CAAAATAACT GTAGTCAGCT TTTAACTTCT GGACCAGCTA CATTGCCAGA TCAGTTGATG
    GCCATAAGTC CACCAGGCCA GCCACAAAAC GAGGGCCAAC CACCTGTGAC AACACTTCTT
    TCTCAGCAAA TGCCAGAGAA TTCTCCAATG GCATCCTCTA TAAACACCAA CCAGAACATT
    GAAAAGATTG ATTTGCTTGT TTCATTGCAA AACCAAGGGA ACAACTTAAC TGGCTCCTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53919
    Clone ID Related Accession (Same CDS sequence) XM_015467397.1 , XM_010814696.2
    Accession Version XM_010814696.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814696.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTATGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA 
    CCTGAGACCC AGCACAGAGC TCGGTACCTG ACTGAGGGTA GTCGTGGCTC AGTAAAGGAC
    AGAACACAGC AAGGCTTTCC TACAGTGAAG CTGGAAGGCC ATAATGAGCC AGTGGTGTTG
    CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC
    AGGGTAACTG GGCGAAATAC AACTCCCTGC AAAGAAGTGG ATATCGAAGG CACTACTGTT
    ATAGAAGTTG GCCTTGATCC AAGCAACAAT ATGACACTGG CGGTTGACTG CGTAGGGATA
    TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAG
    AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACAA GGAAAGATGG CTCCACTTTG
    ACACTGCAAA CACCCTCTTC TCCAATTTTA TGTACTCAAC CAGCAGGAGT GCCAGAAATC
    TTAAAGAAGA GCTTGCATAG CTGTTCGGTG AAAGGAGAGG AAGAAGTATT TTTAATTGGC
    AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT
    TGGAAGTCAG AAGCTGAAAT TGATATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG
    GTTCCCCCAT ATCATGATCA ACATATAACT TTGCCAGTAG CAGTGGGAAT ATATGTAGTA
    ACCAATGCTG GAAGATCTCA TGATGTTCAG CCTTTCACTT ACACTCCAGA CCCAGCAGCT
    GTTGCTTTGA ATGTGAATGT AAAGAAGGAA ATATCTAGTC CGGCAAGACC TTGCTCTTTC
    GAAGAAGCCA TGAAAGCAAT GAAAACTACT GGATGTAACT TAGATAAGGT AAATATGCTT
    CCTAATGCTC TGATCACTCC GCTCATATCA AGCACTATGA TTAAGAGTGA AGACATTACT
    CCAATGGAAG TAACAGCAGA GAAAAGATCG CCTTCTATTT TTAAGACTAC AAAGACAGTT
    GGATCAACTC AACAAACGTT AGAAAATCTC TCTCACATAG CAGGAAATGG GTCTTTTTCA
    TCATCATCTT CCCACTTAAC TTCTGAAAAT GAAAAGCAAC AACAGATTCA GCCCAAGGCA
    TACAACCCAG AGACCCTGAC AACTATCCAA ACACAGGACA TTTCACAGCC TGGTACTTTT
    CCAGCGGTTT CTGCGTCTAG TCAGCTGCCC AGCAATGACG CACTACTACA GCAGGCTACA
    CAGTTTCAAA CAAGAGAAAC TCAGTCTAGA GAGGTGTTAC AGTCAGATGG TACAGTGGTT
    AACTTGTCAC ACCTGACTGA GACATCACAG CAACAGCAGC AGTCTCCACT GCAAGAACAA
    GCACAGACTT TACAGCAGCA GATTTCATCA AATATTTTCC CATCACCAAA TAGTGTGAGT
    CAGCAACTAC AAAATACGAT ACAACACTTG CAGGCAGGGA GTTTTACAGG CAGTACTGCC
    AGTGGCAGCA ATGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAGCAGTTA
    TCTTCAGTTT TATTTTCTGC TCCAGATGGT AACGAGAATG TTCAAGAACA GCTTAGTGCA
    GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGCGTAA GCCCTGGAAT GTTTTCCTCA
    ACAGAACCAG CAGTCCATAC CAGACCAGAT AATTTAATAG CTGGAAGAGC TGAAAGTGTT
    CACCCACAGA ATGAAAACAC ATTGTCTAAT CAGCAACAGC AACAGCAGCA ACAGCAAGTG
    ATGGACTCCT CAGCTGCAAT GGTGATGGAG ATGCAACAGA GTATCTGCCA GGCAGCTGCC
    CAGATTCAGT CAGAGCTGTT TCCTTCATCT GCTTCAGCAA ATGGAAACCT TCAGCAGTCT
    CCAGTTTACC AGCAGACTTC TCATATGATG AGCGCATTAT CAGCCAATGA GGACATGCAA
    ATGCAATGTG AATTGTTTTC TTCTCCTCCG GCAGTTTCTG GAAATGAAAC TACTACAACT
    ACCACACAGC AGGTTGCAAC TTCTGGCACT ACCCTGTTTC AGACATCAAA TTCAGGAGAT
    GGAGAAGAGA CTGGAGCACA AGCAAAACAG ATTCAGAACA GTGTCTTTCA GACCATGGTC
    CAAATGCAAC ATAGTGGGGA CAGTCAGCCT CAAGTTGGCC TTTTTTCATC TACAAAAAGT
    ATGATAAGTG TTCAAAATAG TGGTACCCAG CAGCAAGGCA ATGGTTTATT CCAGCAAGGT
    AATGAGATGA TGTCACTTCA ATCAGGGAAT TTTTTGCAGC AGTCTTCTCA TTCACAGGCT
    CAGCTTTTTC ATCCTCAGAA TCCTATTGCT GATCCTCAGA ACCTTTCCCA GGAAACTCAA
    GGTTCTATTT TTCATAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AGCCTCCTCT
    GAACAAATGC AGCCTCCAAT GTTTCACTCT CAAAATACCA TGGCTGTGCT GCAGGGCTCT
    TCTGTTCCTC AAGACCAACA GTCAGCCAAC ATATTTCTTT CCCAAAGTCC AATGAATAAT
    CTCCAAACCA ACACAGTGGC CCAGGAAGAA CAGATTTCAT TTTTTGCAGC TCAGAATTCA
    ATTTCTCCAC TTCAGTCAAC ATCAAACACT GAGCAGCAAG CTGCTTTCCA GCAGCAGGCT
    CCAATATCAC ACATTCAGAC CCCTATGCTT TCCCAGGAAC AGGCACAACC CTCCCAGCAA
    GGTCTGTTTC AGCCTCAGGT TTCCCTGGGC TCCCTTCCTC CTAACCCAAT GCCTCAAAAC
    CAACAAGGAA CAATCTTTCA GTCACAGCAC TCAATAGTTG CCATCCAGAG TAACTCTCCA
    TCCCAGGAGC AGCAGCAGCA GCAACAACAG CAGCAGCAAC AACAGAGCAT TTTATTCAGT
    AACCAGAACG CCATGGCACC AATGGCGTCT CAAAAGCAGC CACCACCAAA CATGATATTC
    AATCCAAGTC AAAATCCAGT GGCTAATCAG GAGCAGCAGA ACCAATCAAT TTTCCATCAA
    CAAAATAATA TGGCCCCAAT GAATCAAGAG CAGCAGCCCA TGCAATTTCA GAACCAGACC
    ACGGTTTCCT CACTTCAGAA CCCAGGTCCT GCCCAATCCG AATCATCACA GACCTCCTTG
    TTCCATAGCT CACCTCAGAT TCAGTTGGTC CAAGGGTCAC CCAGTTCTCA AGAGCAGCAA
    GTAACACTCT TCCTCTCTCC AGCATCCATG TCTGCATTGC AGACCAGTAT GAACCAACAA
    GACATGCAAC AGTCTCCCCT TTATTCCCCT CAGAACAACA TGCCTGGAAT CCAGGGAGCC
    ACATCTTCAC CTCAACCACA GGCTACTTTA TTTCACAACA CTACAGGAGG TACAATGAAC
    CAGCTACAGA ATTCTCCTGG CTCTTCTCAA CAGACATCTG GAATGTTCTT ATTTGGCATT
    CAAAATAACT GTAGTCAGCT TTTAACTTCT GGACCAGCTA CATTGCCAGA TCAGTTGATG
    GCCATAAGTC CACCAGGCCA GCCACAAAAC GAGGGCCAAC CACCTGTGAC AACACTTCTT
    TCTCAGCAAA TGCCAGAGAA TTCTCCAATG GCATCCTCTA TAAACACCAA CCAGAACATT
    GAAAAGATTG ATTTGCTTGT TTCATTGCAA AACCAAGGGA ACAACTTAAC TGGCTCCTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812998.1
    CDS151..3933
    Translation

    Target ORF information:

    RefSeq Version XM_010814696.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814696.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTATGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA 
    CCTGAGACCC AGCACAGAGC TCGGTACCTG ACTGAGGGTA GTCGTGGCTC AGTAAAGGAC
    AGAACACAGC AAGGCTTTCC TACAGTGAAG CTGGAAGGCC ATAATGAGCC AGTGGTGTTG
    CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC
    AGGGTAACTG GGCGAAATAC AACTCCCTGC AAAGAAGTGG ATATCGAAGG CACTACTGTT
    ATAGAAGTTG GCCTTGATCC AAGCAACAAT ATGACACTGG CGGTTGACTG CGTAGGGATA
    TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAG
    AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACAA GGAAAGATGG CTCCACTTTG
    ACACTGCAAA CACCCTCTTC TCCAATTTTA TGTACTCAAC CAGCAGGAGT GCCAGAAATC
    TTAAAGAAGA GCTTGCATAG CTGTTCGGTG AAAGGAGAGG AAGAAGTATT TTTAATTGGC
    AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT
    TGGAAGTCAG AAGCTGAAAT TGATATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG
    GTTCCCCCAT ATCATGATCA ACATATAACT TTGCCAGTAG CAGTGGGAAT ATATGTAGTA
    ACCAATGCTG GAAGATCTCA TGATGTTCAG CCTTTCACTT ACACTCCAGA CCCAGCAGCT
    GTTGCTTTGA ATGTGAATGT AAAGAAGGAA ATATCTAGTC CGGCAAGACC TTGCTCTTTC
    GAAGAAGCCA TGAAAGCAAT GAAAACTACT GGATGTAACT TAGATAAGGT AAATATGCTT
    CCTAATGCTC TGATCACTCC GCTCATATCA AGCACTATGA TTAAGAGTGA AGACATTACT
    CCAATGGAAG TAACAGCAGA GAAAAGATCG CCTTCTATTT TTAAGACTAC AAAGACAGTT
    GGATCAACTC AACAAACGTT AGAAAATCTC TCTCACATAG CAGGAAATGG GTCTTTTTCA
    TCATCATCTT CCCACTTAAC TTCTGAAAAT GAAAAGCAAC AACAGATTCA GCCCAAGGCA
    TACAACCCAG AGACCCTGAC AACTATCCAA ACACAGGACA TTTCACAGCC TGGTACTTTT
    CCAGCGGTTT CTGCGTCTAG TCAGCTGCCC AGCAATGACG CACTACTACA GCAGGCTACA
    CAGTTTCAAA CAAGAGAAAC TCAGTCTAGA GAGGTGTTAC AGTCAGATGG TACAGTGGTT
    AACTTGTCAC ACCTGACTGA GACATCACAG CAACAGCAGC AGTCTCCACT GCAAGAACAA
    GCACAGACTT TACAGCAGCA GATTTCATCA AATATTTTCC CATCACCAAA TAGTGTGAGT
    CAGCAACTAC AAAATACGAT ACAACACTTG CAGGCAGGGA GTTTTACAGG CAGTACTGCC
    AGTGGCAGCA ATGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAGCAGTTA
    TCTTCAGTTT TATTTTCTGC TCCAGATGGT AACGAGAATG TTCAAGAACA GCTTAGTGCA
    GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGCGTAA GCCCTGGAAT GTTTTCCTCA
    ACAGAACCAG CAGTCCATAC CAGACCAGAT AATTTAATAG CTGGAAGAGC TGAAAGTGTT
    CACCCACAGA ATGAAAACAC ATTGTCTAAT CAGCAACAGC AACAGCAGCA ACAGCAAGTG
    ATGGACTCCT CAGCTGCAAT GGTGATGGAG ATGCAACAGA GTATCTGCCA GGCAGCTGCC
    CAGATTCAGT CAGAGCTGTT TCCTTCATCT GCTTCAGCAA ATGGAAACCT TCAGCAGTCT
    CCAGTTTACC AGCAGACTTC TCATATGATG AGCGCATTAT CAGCCAATGA GGACATGCAA
    ATGCAATGTG AATTGTTTTC TTCTCCTCCG GCAGTTTCTG GAAATGAAAC TACTACAACT
    ACCACACAGC AGGTTGCAAC TTCTGGCACT ACCCTGTTTC AGACATCAAA TTCAGGAGAT
    GGAGAAGAGA CTGGAGCACA AGCAAAACAG ATTCAGAACA GTGTCTTTCA GACCATGGTC
    CAAATGCAAC ATAGTGGGGA CAGTCAGCCT CAAGTTGGCC TTTTTTCATC TACAAAAAGT
    ATGATAAGTG TTCAAAATAG TGGTACCCAG CAGCAAGGCA ATGGTTTATT CCAGCAAGGT
    AATGAGATGA TGTCACTTCA ATCAGGGAAT TTTTTGCAGC AGTCTTCTCA TTCACAGGCT
    CAGCTTTTTC ATCCTCAGAA TCCTATTGCT GATCCTCAGA ACCTTTCCCA GGAAACTCAA
    GGTTCTATTT TTCATAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AGCCTCCTCT
    GAACAAATGC AGCCTCCAAT GTTTCACTCT CAAAATACCA TGGCTGTGCT GCAGGGCTCT
    TCTGTTCCTC AAGACCAACA GTCAGCCAAC ATATTTCTTT CCCAAAGTCC AATGAATAAT
    CTCCAAACCA ACACAGTGGC CCAGGAAGAA CAGATTTCAT TTTTTGCAGC TCAGAATTCA
    ATTTCTCCAC TTCAGTCAAC ATCAAACACT GAGCAGCAAG CTGCTTTCCA GCAGCAGGCT
    CCAATATCAC ACATTCAGAC CCCTATGCTT TCCCAGGAAC AGGCACAACC CTCCCAGCAA
    GGTCTGTTTC AGCCTCAGGT TTCCCTGGGC TCCCTTCCTC CTAACCCAAT GCCTCAAAAC
    CAACAAGGAA CAATCTTTCA GTCACAGCAC TCAATAGTTG CCATCCAGAG TAACTCTCCA
    TCCCAGGAGC AGCAGCAGCA GCAACAACAG CAGCAGCAAC AACAGAGCAT TTTATTCAGT
    AACCAGAACG CCATGGCACC AATGGCGTCT CAAAAGCAGC CACCACCAAA CATGATATTC
    AATCCAAGTC AAAATCCAGT GGCTAATCAG GAGCAGCAGA ACCAATCAAT TTTCCATCAA
    CAAAATAATA TGGCCCCAAT GAATCAAGAG CAGCAGCCCA TGCAATTTCA GAACCAGACC
    ACGGTTTCCT CACTTCAGAA CCCAGGTCCT GCCCAATCCG AATCATCACA GACCTCCTTG
    TTCCATAGCT CACCTCAGAT TCAGTTGGTC CAAGGGTCAC CCAGTTCTCA AGAGCAGCAA
    GTAACACTCT TCCTCTCTCC AGCATCCATG TCTGCATTGC AGACCAGTAT GAACCAACAA
    GACATGCAAC AGTCTCCCCT TTATTCCCCT CAGAACAACA TGCCTGGAAT CCAGGGAGCC
    ACATCTTCAC CTCAACCACA GGCTACTTTA TTTCACAACA CTACAGGAGG TACAATGAAC
    CAGCTACAGA ATTCTCCTGG CTCTTCTCAA CAGACATCTG GAATGTTCTT ATTTGGCATT
    CAAAATAACT GTAGTCAGCT TTTAACTTCT GGACCAGCTA CATTGCCAGA TCAGTTGATG
    GCCATAAGTC CACCAGGCCA GCCACAAAAC GAGGGCCAAC CACCTGTGAC AACACTTCTT
    TCTCAGCAAA TGCCAGAGAA TTCTCCAATG GCATCCTCTA TAAACACCAA CCAGAACATT
    GAAAAGATTG ATTTGCTTGT TTCATTGCAA AACCAAGGGA ACAACTTAAC TGGCTCCTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93417
    Clone ID Related Accession (Same CDS sequence) XM_015475835.1 , XM_015467395.1
    Accession Version XM_015475835.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
    GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT
    TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTCGAGAGC
    TGCTCCTCAG CCTTGGGGGT AAGTAACCGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC
    ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA
    CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA
    TCTGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC
    ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG
    CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC
    GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAGC
    TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA CTGGAGTAAA GAAGAGCCCT
    ATGTTATGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA
    CCTGAGACCC AGCACAGAGC TCGGTACCTG ACTGAGGGTA GTCGTGGCTC AGTAAAGGAC
    AGAACACAGC AAGGCTTTCC TACAGTGAAG CTGGAAGGCC ATAATGAGCC AGTGGTGTTG
    CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC
    AGGGTAACTG GGCGAAATAC AACTCCCTGC AAAGAAGTGG ATATCGAAGG CACTACTGTT
    ATAGAAGTTG GCCTTGATCC AAGCAACAAT ATGACACTGG CGGTTGACTG CGTAGGGATA
    TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAG
    AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACAA GGAAAGATGG CTCCACTTTG
    ACACTGCAAA CACCCTCTTC TCCAATTTTA TGTACTCAAC CAGCAGGAGT GCCAGAAATC
    TTAAAGAAGA GCTTGCATAG CTGTTCGGTG AAAGGAGAGG AAGAAGTATT TTTAATTGGC
    AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT
    TGGAAGTCAG AAGCTGAAAT TGATATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG
    GTTCCCCCAT ATCATGATCA ACATATAACT TTGCCAGTAG CAGTGGGAAT ATATGTAGTA
    ACCAATGCTG GAAGATCTCA TGATGTTCAG CCTTTCACTT ACACTCCAGA CCCAGCAGCT
    GTTGCTTTGA ATGTGAATGT AAAGAAGGAA ATATCTAGTC CGGCAAGACC TTGCTCTTTC
    GAAGAAGCCA TGAAAGCAAT GAAAACTACT GGATGTAACT TAGATAAGGT AAATATGCTT
    CCTAATGCTC TGATCACTCC GCTCATATCA AGCACTATGA TTAAGAGTGA AGACATTACT
    CCAATGGAAG TAACAGCAGA GAAAAGATCG CCTTCTATTT TTAAGACTAC AAAGACAGTT
    GGATCAACTC AACAAACGTT AGAAAATCTC TCTCACATAG CAGGAAATGG GTCTTTTTCA
    TCATCATCTT CCCACTTAAC TTCTGAAAAT GAAAAGCAAC AACAGATTCA GCCCAAGGCA
    TACAACCCAG AGACCCTGAC AACTATCCAA ACACAGGACA TTTCACAGCC TGGTACTTTT
    CCAGCGGTTT CTGCGTCTAG TCAGCTGCCC AGCAATGACG CACTACTACA GCAGGCTACA
    CAGTTTCAAA CAAGAGAAAC TCAGTCTAGA GAGGTGTTAC AGTCAGATGG TACAGTGGTT
    AACTTGTCAC ACCTGACTGA GACATCACAG CAACAGCAGC AGTCTCCACT GCAAGAACAA
    GCACAGACTT TACAGCAGCA GATTTCATCA AATATTTTCC CATCACCAAA TAGTGTGAGT
    CAGCAACTAC AAAATACGAT ACAACACTTG CAGGCAGGGA GTTTTACAGG CAGTACTGCC
    AGTGGCAGCA ATGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAGCAGTTA
    TCTTCAGTTT TATTTTCTGC TCCAGATGGT AACGAGAATG TTCAAGAACA GCTTAGTGCA
    GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGCGTAA GCCCTGGAAT GTTTTCCTCA
    ACAGAACCAG CAGTCCATAC CAGACCAGAT AATTTAATAG CTGGAAGAGC TGAAAGTGTT
    CACCCACAGA ATGAAAACAC ATTGTCTAAT CAGCAACAGC AACAGCAGCA ACAGCAAGTG
    ATGGACTCCT CAGCTGCAAT GGTGATGGAG ATGCAACAGA GTATCTGCCA GGCAGCTGCC
    CAGATTCAGT CAGAGCTGTT TCCTTCATCT GCTTCAGCAA ATGGAAACCT TCAGCAGTCT
    CCAGTTTACC AGCAGACTTC TCATATGATG AGCGCATTAT CAGCCAATGA GGACATGCAA
    ATGCAATGTG AATTGTTTTC TTCTCCTCCG GCAGTTTCTG GAAATGAAAC TACTACAACT
    ACCACACAGC AGGTTGCAAC TTCTGGCACT ACCCTGTTTC AGACATCAAA TTCAGGAGAT
    GGAGAAGAGA CTGGAGCACA AGCAAAACAG ATTCAGAACA GTGTCTTTCA GACCATGGTC
    CAAATGCAAC ATAGTGGGGA CAGTCAGCCT CAAGTTGGCC TTTTTTCATC TACAAAAAGT
    ATGATAAGTG TTCAAAATAG TGGTACCCAG CAGCAAGGCA ATGGTTTATT CCAGCAAGGT
    AATGAGATGA TGTCACTTCA ATCAGGGAAT TTTTTGCAGC AGTCTTCTCA TTCACAGGCT
    CAGCTTTTTC ATCCTCAGAA TCCTATTGCT GATCCTCAGA ACCTTTCCCA GGAAACTCAA
    GGTTCTATTT TTCATAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AGCCTCCTCT
    GAACAAATGC AGCCTCCAAT GTTTCACTCT CAAAATACCA TGGCTGTGCT GCAGGGCTCT
    TCTGTTCCTC AAGACCAACA GTCAGCCAAC ATATTTCTTT CCCAAAGTCC AATGAATAAT
    CTCCAAACCA ACACAGTGGC CCAGGAAGAA CAGATTTCAT TTTTTGCAGC TCAGAATTCA
    ATTTCTCCAC TTCAGTCAAC ATCAAACACT GAGCAGCAAG CTGCTTTCCA GCAGCAGGCT
    CCAATATCAC ACATTCAGAC CCCTATGCTT TCCCAGGAAC AGGCACAACC CTCCCAGCAA
    GGTCTGTTTC AGCCTCAGGT TTCCCTGGGC TCCCTTCCTC CTAACCCAAT GCCTCAAAAC
    CAACAAGGAA CAATCTTTCA GTCACAGCAC TCAATAGTTG CCATCCAGAG TAACTCTCCA
    TCCCAGGAGC AGCAGCAGCA GCAACAACAG CAGCAGCAAC AACAGAGCAT TTTATTCAGT
    AACCAGAACG CCATGGCACC AATGGCGTCT CAAAAGCAGC CACCACCAAA CATGATATTC
    AATCCAAGTC AAAATCCAGT GGCTAATCAG GAGCAGCAGA ACCAATCAAT TTTCCATCAA
    CAAAATAATA TGGCCCCAAT GAATCAAGAG CAGCAGCCCA TGCAATTTCA GAACCAGACC
    ACGGTTTCCT CACTTCAGAA CCCAGGTCCT GCCCAATCCG AATCATCACA GACCTCCTTG
    TTCCATAGCT CACCTCAGAT TCAGTTGGTC CAAGGGTCAC CCAGTTCTCA AGAGCAGCAA
    GTAACACTCT TCCTCTCTCC AGCATCCATG TCTGCATTGC AGACCAGTAT GAACCAACAA
    GACATGCAAC AGTCTCCCCT TTATTCCCCT CAGAACAACA TGCCTGGAAT CCAGGGAGCC
    ACATCTTCAC CTCAACCACA GGCTACTTTA TTTCACAACA CTACAGGAGG TACAATGAAC
    CAGCTACAGA ATTCTCCTGG CTCTTCTCAA CAGACATCTG GAATGTTCTT ATTTGGCATT
    CAAAATAACT GTAGTCAGCT TTTAACTTCT GGACCAGCTA CATTGCCAGA TCAGTTGATG
    GCCATAAGTC CACCAGGCCA GCCACAAAAC GAGGGCCAAC CACCTGTGAC AACACTTCTT
    TCTCAGCAAA TGCCAGAGAA TTCTCCAATG GCATCCTCTA TAAACACCAA CCAGAACATT
    GAAAAGATTG ATTTGCTTGT TTCATTGCAA AACCAAGGGA ACAACTTAAC TGGCTCCTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331321.1
    CDS90..4532
    Translation

    Target ORF information:

    RefSeq Version XM_015475835.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475835.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
    GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT
    TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTCGAGAGC
    TGCTCCTCAG CCTTGGGGGT AAGTAACCGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC
    ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA
    CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA
    TCTGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC
    ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG
    CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC
    GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAGC
    TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA CTGGAGTAAA GAAGAGCCCT
    ATGTTATGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA
    CCTGAGACCC AGCACAGAGC TCGGTACCTG ACTGAGGGTA GTCGTGGCTC AGTAAAGGAC
    AGAACACAGC AAGGCTTTCC TACAGTGAAG CTGGAAGGCC ATAATGAGCC AGTGGTGTTG
    CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC
    AGGGTAACTG GGCGAAATAC AACTCCCTGC AAAGAAGTGG ATATCGAAGG CACTACTGTT
    ATAGAAGTTG GCCTTGATCC AAGCAACAAT ATGACACTGG CGGTTGACTG CGTAGGGATA
    TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAG
    AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACAA GGAAAGATGG CTCCACTTTG
    ACACTGCAAA CACCCTCTTC TCCAATTTTA TGTACTCAAC CAGCAGGAGT GCCAGAAATC
    TTAAAGAAGA GCTTGCATAG CTGTTCGGTG AAAGGAGAGG AAGAAGTATT TTTAATTGGC
    AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT
    TGGAAGTCAG AAGCTGAAAT TGATATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG
    GTTCCCCCAT ATCATGATCA ACATATAACT TTGCCAGTAG CAGTGGGAAT ATATGTAGTA
    ACCAATGCTG GAAGATCTCA TGATGTTCAG CCTTTCACTT ACACTCCAGA CCCAGCAGCT
    GTTGCTTTGA ATGTGAATGT AAAGAAGGAA ATATCTAGTC CGGCAAGACC TTGCTCTTTC
    GAAGAAGCCA TGAAAGCAAT GAAAACTACT GGATGTAACT TAGATAAGGT AAATATGCTT
    CCTAATGCTC TGATCACTCC GCTCATATCA AGCACTATGA TTAAGAGTGA AGACATTACT
    CCAATGGAAG TAACAGCAGA GAAAAGATCG CCTTCTATTT TTAAGACTAC AAAGACAGTT
    GGATCAACTC AACAAACGTT AGAAAATCTC TCTCACATAG CAGGAAATGG GTCTTTTTCA
    TCATCATCTT CCCACTTAAC TTCTGAAAAT GAAAAGCAAC AACAGATTCA GCCCAAGGCA
    TACAACCCAG AGACCCTGAC AACTATCCAA ACACAGGACA TTTCACAGCC TGGTACTTTT
    CCAGCGGTTT CTGCGTCTAG TCAGCTGCCC AGCAATGACG CACTACTACA GCAGGCTACA
    CAGTTTCAAA CAAGAGAAAC TCAGTCTAGA GAGGTGTTAC AGTCAGATGG TACAGTGGTT
    AACTTGTCAC ACCTGACTGA GACATCACAG CAACAGCAGC AGTCTCCACT GCAAGAACAA
    GCACAGACTT TACAGCAGCA GATTTCATCA AATATTTTCC CATCACCAAA TAGTGTGAGT
    CAGCAACTAC AAAATACGAT ACAACACTTG CAGGCAGGGA GTTTTACAGG CAGTACTGCC
    AGTGGCAGCA ATGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAGCAGTTA
    TCTTCAGTTT TATTTTCTGC TCCAGATGGT AACGAGAATG TTCAAGAACA GCTTAGTGCA
    GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGCGTAA GCCCTGGAAT GTTTTCCTCA
    ACAGAACCAG CAGTCCATAC CAGACCAGAT AATTTAATAG CTGGAAGAGC TGAAAGTGTT
    CACCCACAGA ATGAAAACAC ATTGTCTAAT CAGCAACAGC AACAGCAGCA ACAGCAAGTG
    ATGGACTCCT CAGCTGCAAT GGTGATGGAG ATGCAACAGA GTATCTGCCA GGCAGCTGCC
    CAGATTCAGT CAGAGCTGTT TCCTTCATCT GCTTCAGCAA ATGGAAACCT TCAGCAGTCT
    CCAGTTTACC AGCAGACTTC TCATATGATG AGCGCATTAT CAGCCAATGA GGACATGCAA
    ATGCAATGTG AATTGTTTTC TTCTCCTCCG GCAGTTTCTG GAAATGAAAC TACTACAACT
    ACCACACAGC AGGTTGCAAC TTCTGGCACT ACCCTGTTTC AGACATCAAA TTCAGGAGAT
    GGAGAAGAGA CTGGAGCACA AGCAAAACAG ATTCAGAACA GTGTCTTTCA GACCATGGTC
    CAAATGCAAC ATAGTGGGGA CAGTCAGCCT CAAGTTGGCC TTTTTTCATC TACAAAAAGT
    ATGATAAGTG TTCAAAATAG TGGTACCCAG CAGCAAGGCA ATGGTTTATT CCAGCAAGGT
    AATGAGATGA TGTCACTTCA ATCAGGGAAT TTTTTGCAGC AGTCTTCTCA TTCACAGGCT
    CAGCTTTTTC ATCCTCAGAA TCCTATTGCT GATCCTCAGA ACCTTTCCCA GGAAACTCAA
    GGTTCTATTT TTCATAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AGCCTCCTCT
    GAACAAATGC AGCCTCCAAT GTTTCACTCT CAAAATACCA TGGCTGTGCT GCAGGGCTCT
    TCTGTTCCTC AAGACCAACA GTCAGCCAAC ATATTTCTTT CCCAAAGTCC AATGAATAAT
    CTCCAAACCA ACACAGTGGC CCAGGAAGAA CAGATTTCAT TTTTTGCAGC TCAGAATTCA
    ATTTCTCCAC TTCAGTCAAC ATCAAACACT GAGCAGCAAG CTGCTTTCCA GCAGCAGGCT
    CCAATATCAC ACATTCAGAC CCCTATGCTT TCCCAGGAAC AGGCACAACC CTCCCAGCAA
    GGTCTGTTTC AGCCTCAGGT TTCCCTGGGC TCCCTTCCTC CTAACCCAAT GCCTCAAAAC
    CAACAAGGAA CAATCTTTCA GTCACAGCAC TCAATAGTTG CCATCCAGAG TAACTCTCCA
    TCCCAGGAGC AGCAGCAGCA GCAACAACAG CAGCAGCAAC AACAGAGCAT TTTATTCAGT
    AACCAGAACG CCATGGCACC AATGGCGTCT CAAAAGCAGC CACCACCAAA CATGATATTC
    AATCCAAGTC AAAATCCAGT GGCTAATCAG GAGCAGCAGA ACCAATCAAT TTTCCATCAA
    CAAAATAATA TGGCCCCAAT GAATCAAGAG CAGCAGCCCA TGCAATTTCA GAACCAGACC
    ACGGTTTCCT CACTTCAGAA CCCAGGTCCT GCCCAATCCG AATCATCACA GACCTCCTTG
    TTCCATAGCT CACCTCAGAT TCAGTTGGTC CAAGGGTCAC CCAGTTCTCA AGAGCAGCAA
    GTAACACTCT TCCTCTCTCC AGCATCCATG TCTGCATTGC AGACCAGTAT GAACCAACAA
    GACATGCAAC AGTCTCCCCT TTATTCCCCT CAGAACAACA TGCCTGGAAT CCAGGGAGCC
    ACATCTTCAC CTCAACCACA GGCTACTTTA TTTCACAACA CTACAGGAGG TACAATGAAC
    CAGCTACAGA ATTCTCCTGG CTCTTCTCAA CAGACATCTG GAATGTTCTT ATTTGGCATT
    CAAAATAACT GTAGTCAGCT TTTAACTTCT GGACCAGCTA CATTGCCAGA TCAGTTGATG
    GCCATAAGTC CACCAGGCCA GCCACAAAAC GAGGGCCAAC CACCTGTGAC AACACTTCTT
    TCTCAGCAAA TGCCAGAGAA TTCTCCAATG GCATCCTCTA TAAACACCAA CCAGAACATT
    GAAAAGATTG ATTTGCTTGT TTCATTGCAA AACCAAGGGA ACAACTTAAC TGGCTCCTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93417
    Clone ID Related Accession (Same CDS sequence) XM_015475835.1 , XM_015467395.1
    Accession Version XM_015467395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
    GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT
    TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTCGAGAGC
    TGCTCCTCAG CCTTGGGGGT AAGTAACCGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC
    ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA
    CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA
    TCTGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC
    ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG
    CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC
    GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAGC
    TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA CTGGAGTAAA GAAGAGCCCT
    ATGTTATGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA
    CCTGAGACCC AGCACAGAGC TCGGTACCTG ACTGAGGGTA GTCGTGGCTC AGTAAAGGAC
    AGAACACAGC AAGGCTTTCC TACAGTGAAG CTGGAAGGCC ATAATGAGCC AGTGGTGTTG
    CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC
    AGGGTAACTG GGCGAAATAC AACTCCCTGC AAAGAAGTGG ATATCGAAGG CACTACTGTT
    ATAGAAGTTG GCCTTGATCC AAGCAACAAT ATGACACTGG CGGTTGACTG CGTAGGGATA
    TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAG
    AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACAA GGAAAGATGG CTCCACTTTG
    ACACTGCAAA CACCCTCTTC TCCAATTTTA TGTACTCAAC CAGCAGGAGT GCCAGAAATC
    TTAAAGAAGA GCTTGCATAG CTGTTCGGTG AAAGGAGAGG AAGAAGTATT TTTAATTGGC
    AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT
    TGGAAGTCAG AAGCTGAAAT TGATATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG
    GTTCCCCCAT ATCATGATCA ACATATAACT TTGCCAGTAG CAGTGGGAAT ATATGTAGTA
    ACCAATGCTG GAAGATCTCA TGATGTTCAG CCTTTCACTT ACACTCCAGA CCCAGCAGCT
    GTTGCTTTGA ATGTGAATGT AAAGAAGGAA ATATCTAGTC CGGCAAGACC TTGCTCTTTC
    GAAGAAGCCA TGAAAGCAAT GAAAACTACT GGATGTAACT TAGATAAGGT AAATATGCTT
    CCTAATGCTC TGATCACTCC GCTCATATCA AGCACTATGA TTAAGAGTGA AGACATTACT
    CCAATGGAAG TAACAGCAGA GAAAAGATCG CCTTCTATTT TTAAGACTAC AAAGACAGTT
    GGATCAACTC AACAAACGTT AGAAAATCTC TCTCACATAG CAGGAAATGG GTCTTTTTCA
    TCATCATCTT CCCACTTAAC TTCTGAAAAT GAAAAGCAAC AACAGATTCA GCCCAAGGCA
    TACAACCCAG AGACCCTGAC AACTATCCAA ACACAGGACA TTTCACAGCC TGGTACTTTT
    CCAGCGGTTT CTGCGTCTAG TCAGCTGCCC AGCAATGACG CACTACTACA GCAGGCTACA
    CAGTTTCAAA CAAGAGAAAC TCAGTCTAGA GAGGTGTTAC AGTCAGATGG TACAGTGGTT
    AACTTGTCAC ACCTGACTGA GACATCACAG CAACAGCAGC AGTCTCCACT GCAAGAACAA
    GCACAGACTT TACAGCAGCA GATTTCATCA AATATTTTCC CATCACCAAA TAGTGTGAGT
    CAGCAACTAC AAAATACGAT ACAACACTTG CAGGCAGGGA GTTTTACAGG CAGTACTGCC
    AGTGGCAGCA ATGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAGCAGTTA
    TCTTCAGTTT TATTTTCTGC TCCAGATGGT AACGAGAATG TTCAAGAACA GCTTAGTGCA
    GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGCGTAA GCCCTGGAAT GTTTTCCTCA
    ACAGAACCAG CAGTCCATAC CAGACCAGAT AATTTAATAG CTGGAAGAGC TGAAAGTGTT
    CACCCACAGA ATGAAAACAC ATTGTCTAAT CAGCAACAGC AACAGCAGCA ACAGCAAGTG
    ATGGACTCCT CAGCTGCAAT GGTGATGGAG ATGCAACAGA GTATCTGCCA GGCAGCTGCC
    CAGATTCAGT CAGAGCTGTT TCCTTCATCT GCTTCAGCAA ATGGAAACCT TCAGCAGTCT
    CCAGTTTACC AGCAGACTTC TCATATGATG AGCGCATTAT CAGCCAATGA GGACATGCAA
    ATGCAATGTG AATTGTTTTC TTCTCCTCCG GCAGTTTCTG GAAATGAAAC TACTACAACT
    ACCACACAGC AGGTTGCAAC TTCTGGCACT ACCCTGTTTC AGACATCAAA TTCAGGAGAT
    GGAGAAGAGA CTGGAGCACA AGCAAAACAG ATTCAGAACA GTGTCTTTCA GACCATGGTC
    CAAATGCAAC ATAGTGGGGA CAGTCAGCCT CAAGTTGGCC TTTTTTCATC TACAAAAAGT
    ATGATAAGTG TTCAAAATAG TGGTACCCAG CAGCAAGGCA ATGGTTTATT CCAGCAAGGT
    AATGAGATGA TGTCACTTCA ATCAGGGAAT TTTTTGCAGC AGTCTTCTCA TTCACAGGCT
    CAGCTTTTTC ATCCTCAGAA TCCTATTGCT GATCCTCAGA ACCTTTCCCA GGAAACTCAA
    GGTTCTATTT TTCATAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AGCCTCCTCT
    GAACAAATGC AGCCTCCAAT GTTTCACTCT CAAAATACCA TGGCTGTGCT GCAGGGCTCT
    TCTGTTCCTC AAGACCAACA GTCAGCCAAC ATATTTCTTT CCCAAAGTCC AATGAATAAT
    CTCCAAACCA ACACAGTGGC CCAGGAAGAA CAGATTTCAT TTTTTGCAGC TCAGAATTCA
    ATTTCTCCAC TTCAGTCAAC ATCAAACACT GAGCAGCAAG CTGCTTTCCA GCAGCAGGCT
    CCAATATCAC ACATTCAGAC CCCTATGCTT TCCCAGGAAC AGGCACAACC CTCCCAGCAA
    GGTCTGTTTC AGCCTCAGGT TTCCCTGGGC TCCCTTCCTC CTAACCCAAT GCCTCAAAAC
    CAACAAGGAA CAATCTTTCA GTCACAGCAC TCAATAGTTG CCATCCAGAG TAACTCTCCA
    TCCCAGGAGC AGCAGCAGCA GCAACAACAG CAGCAGCAAC AACAGAGCAT TTTATTCAGT
    AACCAGAACG CCATGGCACC AATGGCGTCT CAAAAGCAGC CACCACCAAA CATGATATTC
    AATCCAAGTC AAAATCCAGT GGCTAATCAG GAGCAGCAGA ACCAATCAAT TTTCCATCAA
    CAAAATAATA TGGCCCCAAT GAATCAAGAG CAGCAGCCCA TGCAATTTCA GAACCAGACC
    ACGGTTTCCT CACTTCAGAA CCCAGGTCCT GCCCAATCCG AATCATCACA GACCTCCTTG
    TTCCATAGCT CACCTCAGAT TCAGTTGGTC CAAGGGTCAC CCAGTTCTCA AGAGCAGCAA
    GTAACACTCT TCCTCTCTCC AGCATCCATG TCTGCATTGC AGACCAGTAT GAACCAACAA
    GACATGCAAC AGTCTCCCCT TTATTCCCCT CAGAACAACA TGCCTGGAAT CCAGGGAGCC
    ACATCTTCAC CTCAACCACA GGCTACTTTA TTTCACAACA CTACAGGAGG TACAATGAAC
    CAGCTACAGA ATTCTCCTGG CTCTTCTCAA CAGACATCTG GAATGTTCTT ATTTGGCATT
    CAAAATAACT GTAGTCAGCT TTTAACTTCT GGACCAGCTA CATTGCCAGA TCAGTTGATG
    GCCATAAGTC CACCAGGCCA GCCACAAAAC GAGGGCCAAC CACCTGTGAC AACACTTCTT
    TCTCAGCAAA TGCCAGAGAA TTCTCCAATG GCATCCTCTA TAAACACCAA CCAGAACATT
    GAAAAGATTG ATTTGCTTGT TTCATTGCAA AACCAAGGGA ACAACTTAAC TGGCTCCTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322881.1
    CDS90..4532
    Translation

    Target ORF information:

    RefSeq Version XM_015467395.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTCAGA CAAGTGGTGG TGAGGCAGGC TCGCCTCCTC CAGCTGTAGT TGCTGCTGAT 
    GCTTCTTCAG CTCCCTCCTC TTCCTCCATG GGCGGTGCTT GCAGCTCCTT TACCACCTCT
    TCCAGCCCTA CCATTTATTC TACCTCAGTC ACCGACAGCA AGGCTATGCA AGTCGAGAGC
    TGCTCCTCAG CCTTGGGGGT AAGTAACCGA GGGGTAAGTG AAAAGCAGTT AACCAGTAAC
    ACAGTTCAGC AGCATCCATC AACACCGAAG AGGCACACAG TCTTGTACAT CTCACCACCA
    CCTGAGGACT TGCTGGATAA CAGTCGGATG TCCTGCCAGG ATGAGGGGTG TGGATTGGAA
    TCTGAGCAGA GCTGCAGTAT GTGGATGGAG GATTCCCCCT CCAACTTCAG TAACATGAGC
    ACCAGTTCCT ACAATGATAA CACTGAGGTA CCTCGTAAAT CACGAAAACG AAATCCAAAG
    CAGAGGCCGG GGGTCAAACG ACGAGATTGT GAAGAATCTA ATATGGATAT ATTTGATGCC
    GACAGTGCCA AAGCACCTCA CTATGTGCTT TCTCAGCTTA CCACGGACAA CAAAGGCAGC
    TCAAAAGCAG GAAATGGAAC ATTGGAAAAC CAAAAAGGAA CTGGAGTAAA GAAGAGCCCT
    ATGTTATGTG GACAGTATCC TGTTAAAAGT GAGGGAAAGG AGCTGAAGAT AGTTGTACAA
    CCTGAGACCC AGCACAGAGC TCGGTACCTG ACTGAGGGTA GTCGTGGCTC AGTAAAGGAC
    AGAACACAGC AAGGCTTTCC TACAGTGAAG CTGGAAGGCC ATAATGAGCC AGTGGTGTTG
    CAGGTGTTTG TGGGCAATGA CTCTGGTCGA GTGAAACCAC ATGGATTTTA TCAGGCCTGC
    AGGGTAACTG GGCGAAATAC AACTCCCTGC AAAGAAGTGG ATATCGAAGG CACTACTGTT
    ATAGAAGTTG GCCTTGATCC AAGCAACAAT ATGACACTGG CGGTTGACTG CGTAGGGATA
    TTGAAATTGA GGAATGCTGA TGTTGAAGCC AGAATAGGAA TTGCTGGTTC CAAGAAAAAG
    AGCACTCGTG CCAGATTGGT TTTTCGAGTT AATATCACAA GGAAAGATGG CTCCACTTTG
    ACACTGCAAA CACCCTCTTC TCCAATTTTA TGTACTCAAC CAGCAGGAGT GCCAGAAATC
    TTAAAGAAGA GCTTGCATAG CTGTTCGGTG AAAGGAGAGG AAGAAGTATT TTTAATTGGC
    AAGAACTTTC TGAAAGGAAC TAAAGTTATT TTCCAAGAAA ATGTTTCTGA TGAAAACTCT
    TGGAAGTCAG AAGCTGAAAT TGATATGGAA TTATTTCATC AGAATCATCT TATTGTGAAG
    GTTCCCCCAT ATCATGATCA ACATATAACT TTGCCAGTAG CAGTGGGAAT ATATGTAGTA
    ACCAATGCTG GAAGATCTCA TGATGTTCAG CCTTTCACTT ACACTCCAGA CCCAGCAGCT
    GTTGCTTTGA ATGTGAATGT AAAGAAGGAA ATATCTAGTC CGGCAAGACC TTGCTCTTTC
    GAAGAAGCCA TGAAAGCAAT GAAAACTACT GGATGTAACT TAGATAAGGT AAATATGCTT
    CCTAATGCTC TGATCACTCC GCTCATATCA AGCACTATGA TTAAGAGTGA AGACATTACT
    CCAATGGAAG TAACAGCAGA GAAAAGATCG CCTTCTATTT TTAAGACTAC AAAGACAGTT
    GGATCAACTC AACAAACGTT AGAAAATCTC TCTCACATAG CAGGAAATGG GTCTTTTTCA
    TCATCATCTT CCCACTTAAC TTCTGAAAAT GAAAAGCAAC AACAGATTCA GCCCAAGGCA
    TACAACCCAG AGACCCTGAC AACTATCCAA ACACAGGACA TTTCACAGCC TGGTACTTTT
    CCAGCGGTTT CTGCGTCTAG TCAGCTGCCC AGCAATGACG CACTACTACA GCAGGCTACA
    CAGTTTCAAA CAAGAGAAAC TCAGTCTAGA GAGGTGTTAC AGTCAGATGG TACAGTGGTT
    AACTTGTCAC ACCTGACTGA GACATCACAG CAACAGCAGC AGTCTCCACT GCAAGAACAA
    GCACAGACTT TACAGCAGCA GATTTCATCA AATATTTTCC CATCACCAAA TAGTGTGAGT
    CAGCAACTAC AAAATACGAT ACAACACTTG CAGGCAGGGA GTTTTACAGG CAGTACTGCC
    AGTGGCAGCA ATGGAAATGT TGACTTGGTC CAACAAGTTT TAGAGGCACA ACAGCAGTTA
    TCTTCAGTTT TATTTTCTGC TCCAGATGGT AACGAGAATG TTCAAGAACA GCTTAGTGCA
    GACATTTTTC AACAAGTCAG TCAAATTCAA AATAGCGTAA GCCCTGGAAT GTTTTCCTCA
    ACAGAACCAG CAGTCCATAC CAGACCAGAT AATTTAATAG CTGGAAGAGC TGAAAGTGTT
    CACCCACAGA ATGAAAACAC ATTGTCTAAT CAGCAACAGC AACAGCAGCA ACAGCAAGTG
    ATGGACTCCT CAGCTGCAAT GGTGATGGAG ATGCAACAGA GTATCTGCCA GGCAGCTGCC
    CAGATTCAGT CAGAGCTGTT TCCTTCATCT GCTTCAGCAA ATGGAAACCT TCAGCAGTCT
    CCAGTTTACC AGCAGACTTC TCATATGATG AGCGCATTAT CAGCCAATGA GGACATGCAA
    ATGCAATGTG AATTGTTTTC TTCTCCTCCG GCAGTTTCTG GAAATGAAAC TACTACAACT
    ACCACACAGC AGGTTGCAAC TTCTGGCACT ACCCTGTTTC AGACATCAAA TTCAGGAGAT
    GGAGAAGAGA CTGGAGCACA AGCAAAACAG ATTCAGAACA GTGTCTTTCA GACCATGGTC
    CAAATGCAAC ATAGTGGGGA CAGTCAGCCT CAAGTTGGCC TTTTTTCATC TACAAAAAGT
    ATGATAAGTG TTCAAAATAG TGGTACCCAG CAGCAAGGCA ATGGTTTATT CCAGCAAGGT
    AATGAGATGA TGTCACTTCA ATCAGGGAAT TTTTTGCAGC AGTCTTCTCA TTCACAGGCT
    CAGCTTTTTC ATCCTCAGAA TCCTATTGCT GATCCTCAGA ACCTTTCCCA GGAAACTCAA
    GGTTCTATTT TTCATAGTCC AAGTCCTATT GTCCACAGTC AGACTTCTAC AGCCTCCTCT
    GAACAAATGC AGCCTCCAAT GTTTCACTCT CAAAATACCA TGGCTGTGCT GCAGGGCTCT
    TCTGTTCCTC AAGACCAACA GTCAGCCAAC ATATTTCTTT CCCAAAGTCC AATGAATAAT
    CTCCAAACCA ACACAGTGGC CCAGGAAGAA CAGATTTCAT TTTTTGCAGC TCAGAATTCA
    ATTTCTCCAC TTCAGTCAAC ATCAAACACT GAGCAGCAAG CTGCTTTCCA GCAGCAGGCT
    CCAATATCAC ACATTCAGAC CCCTATGCTT TCCCAGGAAC AGGCACAACC CTCCCAGCAA
    GGTCTGTTTC AGCCTCAGGT TTCCCTGGGC TCCCTTCCTC CTAACCCAAT GCCTCAAAAC
    CAACAAGGAA CAATCTTTCA GTCACAGCAC TCAATAGTTG CCATCCAGAG TAACTCTCCA
    TCCCAGGAGC AGCAGCAGCA GCAACAACAG CAGCAGCAAC AACAGAGCAT TTTATTCAGT
    AACCAGAACG CCATGGCACC AATGGCGTCT CAAAAGCAGC CACCACCAAA CATGATATTC
    AATCCAAGTC AAAATCCAGT GGCTAATCAG GAGCAGCAGA ACCAATCAAT TTTCCATCAA
    CAAAATAATA TGGCCCCAAT GAATCAAGAG CAGCAGCCCA TGCAATTTCA GAACCAGACC
    ACGGTTTCCT CACTTCAGAA CCCAGGTCCT GCCCAATCCG AATCATCACA GACCTCCTTG
    TTCCATAGCT CACCTCAGAT TCAGTTGGTC CAAGGGTCAC CCAGTTCTCA AGAGCAGCAA
    GTAACACTCT TCCTCTCTCC AGCATCCATG TCTGCATTGC AGACCAGTAT GAACCAACAA
    GACATGCAAC AGTCTCCCCT TTATTCCCCT CAGAACAACA TGCCTGGAAT CCAGGGAGCC
    ACATCTTCAC CTCAACCACA GGCTACTTTA TTTCACAACA CTACAGGAGG TACAATGAAC
    CAGCTACAGA ATTCTCCTGG CTCTTCTCAA CAGACATCTG GAATGTTCTT ATTTGGCATT
    CAAAATAACT GTAGTCAGCT TTTAACTTCT GGACCAGCTA CATTGCCAGA TCAGTTGATG
    GCCATAAGTC CACCAGGCCA GCCACAAAAC GAGGGCCAAC CACCTGTGAC AACACTTCTT
    TCTCAGCAAA TGCCAGAGAA TTCTCCAATG GCATCCTCTA TAAACACCAA CCAGAACATT
    GAAAAGATTG ATTTGCTTGT TTCATTGCAA AACCAAGGGA ACAACTTAAC TGGCTCCTTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225503
    Clone ID Related Accession (Same CDS sequence) XM_002694839.6
    Accession Version XM_002694839.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: nuclear factor of activated T-cells 5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694839.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTN CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CAGCTGTTGC TTTGAATGTG AATGTAAAGA AGGAAATATC TAGTCCGGCA
    AGACCTTGCT CTTTCGAAGA AGCCATGAAA GCAATGAAAA CTACTGGATG TAACTTAGAT
    AAGGTAAATA TGCTTCCTAA TGCTCTGATC ACTCCGCTCA TATCAAGCAC TATGATTAAG
    AGTGAAGACA TTACTCCAAT GGAAGTAACA GCAGAGAAAA GATCGCCTTC TATTTTTAAG
    ACTACAAAGA CAGTTGGATC AACTCAACAA ACGTTAGAAA ATCTCTCTCA CATAGCAGGA
    AATGGGTCTT TTTCATCATC ATCTTCCCAC TTAACTTCTG AAAATGAAAA GCAACAACAG
    ATTCAGCCCA AGGCATACAA CCCAGAGACC CTGACAACTA TCCAAACACA GGACATTTCA
    CAGCCTGGTA CTTTTCCAGC GGTTTCTGCG TCTAGTCAGC TGCCCAGCAA TGACGCACTA
    CTACAGCAGG CTACACAGTT TCAAACAAGA GAAACTCAGT CTAGAGAGGT GTTACAGTCA
    GATGGTACAG TGGTTAACTT GTCACACCTG ACTGAGACAT CACAGCAACA GCAGCAGTCT
    CCACTGCAAG AACAAGCACA GACTTTACAG CAGCAGATTT CATCAAATAT TTTCCCATCA
    CCAAATAGTG TGAGTCAGCA ACTACAAAAT ACGATACAAC ACTTGCAGGC AGGGAGTTTT
    ACAGGCAGTA CTGCCAGTGG CAGCAATGGA AATGTTGACT TGGTCCAACA AGTTTTAGAG
    GCACAACAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAACGA GAATGTTCAA
    GAACAGCTTA GTGCAGACAT TTTTCAACAA GTCAGTCAAA TTCAAAATAG CGTAAGCCCT
    GGAATGTTTT CCTCAACAGA ACCAGCAGTC CATACCAGAC CAGATAATTT AATAGCTGGA
    AGAGCTGAAA GTGTTCACCC ACAGAATGAA AACACATTGT CTAATCAGCA ACAGCAACAG
    CAGCAACAGC AAGTGATGGA CTCCTCAGCT GCAATGGTGA TGGAGATGCA ACAGAGTATC
    TGCCAGGCAG CTGCCCAGAT TCAGTCAGAG CTGTTTCCTT CATCTGCTTC AGCAAATGGA
    AACCTTCAGC AGTCTCCAGT TTACCAGCAG ACTTCTCATA TGATGAGCGC ATTATCAGCC
    AATGAGGACA TGCAAATGCA ATGTGAATTG TTTTCTTCTC CTCCGGCAGT TTCTGGAAAT
    GAAACTACTA CAACTACCAC ACAGCAGGTT GCAACTTCTG GCACTACCCT GTTTCAGACA
    TCAAATTCAG GAGATGGAGA AGAGACTGGA GCACAAGCAA AACAGATTCA GAACAGTGTC
    TTTCAGACCA TGGTCCAAAT GCAACATAGT GGGGACAGTC AGCCTCAAGT TGGCCTTTTT
    TCATCTACAA AAAGTATGAT AAGTGTTCAA AATAGTGGTA CCCAGCAGCA AGGCAATGGT
    TTATTCCAGC AAGGTAATGA GATGATGTCA CTTCAATCAG GGAATTTTTT GCAGCAGTCT
    TCTCATTCAC AGGCTCAGCT TTTTCATCCT CAGAATCCTA TTGCTGATCC TCAGAACCTT
    TCCCAGGAAA CTCAAGGTTC TATTTTTCAT AGTCCAAGTC CTATTGTCCA CAGTCAGACT
    TCTACAGCCT CCTCTGAACA AATGCAGCCT CCAATGTTTC ACTCTCAAAA TACCATGGCT
    GTGCTGCAGG GCTCTTCTGT TCCTCAAGAC CAACAGTCAG CCAACATATT TCTTTCCCAA
    AGTCCAATGA ATAATCTCCA AACCAACACA GTGGCCCAGG AAGAACAGAT TTCATTTTTT
    GCAGCTCAGA ATTCAATTTC TCCACTTCAG TCAACATCAA ACACTGAGCA GCAAGCTGCT
    TTCCAGCAGC AGGCTCCAAT ATCACACATT CAGACCCCTA TGCTTTCCCA GGAACAGGCA
    CAACCCTCCC AGCAAGGTCT GTTTCAGCCT CAGGTTTCCC TGGGCTCCCT TCCTCCTAAC
    CCAATGCCTC AAAACCAACA AGGAACAATC TTTCAGTCAC AGCACTCAAT AGTTGCCATC
    CAGAGTAACT CTCCATCCCA GGAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAACAACAG
    AGCATTTTAT TCAGTAACCA GAACGCCATG GCACCAATGG CGTCTCAAAA GCAGCCACCA
    CCAAACATGA TATTCAATCC AAGTCAAAAT CCAGTGGCTA ATCAGGAGCA GCAGAACCAA
    TCAATTTTCC ATCAACAAAA TAATATGGCC CCAATGAATC AAGAGCAGCA GCCCATGCAA
    TTTCAGAACC AGACCACGGT TTCCTCACTT CAGAACCCAG GTCCTGCCCA ATCCGAATCA
    TCACAGACCT CCTTGTTCCA TAGCTCACCT CAGATTCAGT TGGTCCAAGG GTCACCCAGT
    TCTCAAGAGC AGCAAGTAAC ACTCTTCCTC TCTCCAGCAT CCATGTCTGC ATTGCAGACC
    AGTATGAACC AACAAGACAT GCAACAGTCT CCCCTTTATT CCCCTCAGAA CAACATGCCT
    GGAATCCAGG GAGCCACATC TTCACCTCAA CCACAGGCTA CTTTATTTCA CAACACTACA
    GGAGGTACAA TGAACCAGCT ACAGAATTCT CCTGGCTCTT CTCAACAGAC ATCTGGAATG
    TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CTTCTGGACC AGCTACATTG
    CCAGATCAGT TGATGGCCAT AAGTCCACCA GGCCAGCCAC AAAACGAGGG CCAACCACCT
    GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CAATGGCATC CTCTATAAAC
    ACCAACCAGA ACATTGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC
    TTAACTGGCT CCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694885.2
    CDS103..4740
    Translation

    Target ORF information:

    RefSeq Version XM_002694839.6
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T cells 5 (NFAT5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694839.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTN CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CAGCTGTTGC TTTGAATGTG AATGTAAAGA AGGAAATATC TAGTCCGGCA
    AGACCTTGCT CTTTCGAAGA AGCCATGAAA GCAATGAAAA CTACTGGATG TAACTTAGAT
    AAGGTAAATA TGCTTCCTAA TGCTCTGATC ACTCCGCTCA TATCAAGCAC TATGATTAAG
    AGTGAAGACA TTACTCCAAT GGAAGTAACA GCAGAGAAAA GATCGCCTTC TATTTTTAAG
    ACTACAAAGA CAGTTGGATC AACTCAACAA ACGTTAGAAA ATCTCTCTCA CATAGCAGGA
    AATGGGTCTT TTTCATCATC ATCTTCCCAC TTAACTTCTG AAAATGAAAA GCAACAACAG
    ATTCAGCCCA AGGCATACAA CCCAGAGACC CTGACAACTA TCCAAACACA GGACATTTCA
    CAGCCTGGTA CTTTTCCAGC GGTTTCTGCG TCTAGTCAGC TGCCCAGCAA TGACGCACTA
    CTACAGCAGG CTACACAGTT TCAAACAAGA GAAACTCAGT CTAGAGAGGT GTTACAGTCA
    GATGGTACAG TGGTTAACTT GTCACACCTG ACTGAGACAT CACAGCAACA GCAGCAGTCT
    CCACTGCAAG AACAAGCACA GACTTTACAG CAGCAGATTT CATCAAATAT TTTCCCATCA
    CCAAATAGTG TGAGTCAGCA ACTACAAAAT ACGATACAAC ACTTGCAGGC AGGGAGTTTT
    ACAGGCAGTA CTGCCAGTGG CAGCAATGGA AATGTTGACT TGGTCCAACA AGTTTTAGAG
    GCACAACAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAACGA GAATGTTCAA
    GAACAGCTTA GTGCAGACAT TTTTCAACAA GTCAGTCAAA TTCAAAATAG CGTAAGCCCT
    GGAATGTTTT CCTCAACAGA ACCAGCAGTC CATACCAGAC CAGATAATTT AATAGCTGGA
    AGAGCTGAAA GTGTTCACCC ACAGAATGAA AACACATTGT CTAATCAGCA ACAGCAACAG
    CAGCAACAGC AAGTGATGGA CTCCTCAGCT GCAATGGTGA TGGAGATGCA ACAGAGTATC
    TGCCAGGCAG CTGCCCAGAT TCAGTCAGAG CTGTTTCCTT CATCTGCTTC AGCAAATGGA
    AACCTTCAGC AGTCTCCAGT TTACCAGCAG ACTTCTCATA TGATGAGCGC ATTATCAGCC
    AATGAGGACA TGCAAATGCA ATGTGAATTG TTTTCTTCTC CTCCGGCAGT TTCTGGAAAT
    GAAACTACTA CAACTACCAC ACAGCAGGTT GCAACTTCTG GCACTACCCT GTTTCAGACA
    TCAAATTCAG GAGATGGAGA AGAGACTGGA GCACAAGCAA AACAGATTCA GAACAGTGTC
    TTTCAGACCA TGGTCCAAAT GCAACATAGT GGGGACAGTC AGCCTCAAGT TGGCCTTTTT
    TCATCTACAA AAAGTATGAT AAGTGTTCAA AATAGTGGTA CCCAGCAGCA AGGCAATGGT
    TTATTCCAGC AAGGTAATGA GATGATGTCA CTTCAATCAG GGAATTTTTT GCAGCAGTCT
    TCTCATTCAC AGGCTCAGCT TTTTCATCCT CAGAATCCTA TTGCTGATCC TCAGAACCTT
    TCCCAGGAAA CTCAAGGTTC TATTTTTCAT AGTCCAAGTC CTATTGTCCA CAGTCAGACT
    TCTACAGCCT CCTCTGAACA AATGCAGCCT CCAATGTTTC ACTCTCAAAA TACCATGGCT
    GTGCTGCAGG GCTCTTCTGT TCCTCAAGAC CAACAGTCAG CCAACATATT TCTTTCCCAA
    AGTCCAATGA ATAATCTCCA AACCAACACA GTGGCCCAGG AAGAACAGAT TTCATTTTTT
    GCAGCTCAGA ATTCAATTTC TCCACTTCAG TCAACATCAA ACACTGAGCA GCAAGCTGCT
    TTCCAGCAGC AGGCTCCAAT ATCACACATT CAGACCCCTA TGCTTTCCCA GGAACAGGCA
    CAACCCTCCC AGCAAGGTCT GTTTCAGCCT CAGGTTTCCC TGGGCTCCCT TCCTCCTAAC
    CCAATGCCTC AAAACCAACA AGGAACAATC TTTCAGTCAC AGCACTCAAT AGTTGCCATC
    CAGAGTAACT CTCCATCCCA GGAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAACAACAG
    AGCATTTTAT TCAGTAACCA GAACGCCATG GCACCAATGG CGTCTCAAAA GCAGCCACCA
    CCAAACATGA TATTCAATCC AAGTCAAAAT CCAGTGGCTA ATCAGGAGCA GCAGAACCAA
    TCAATTTTCC ATCAACAAAA TAATATGGCC CCAATGAATC AAGAGCAGCA GCCCATGCAA
    TTTCAGAACC AGACCACGGT TTCCTCACTT CAGAACCCAG GTCCTGCCCA ATCCGAATCA
    TCACAGACCT CCTTGTTCCA TAGCTCACCT CAGATTCAGT TGGTCCAAGG GTCACCCAGT
    TCTCAAGAGC AGCAAGTAAC ACTCTTCCTC TCTCCAGCAT CCATGTCTGC ATTGCAGACC
    AGTATGAACC AACAAGACAT GCAACAGTCT CCCCTTTATT CCCCTCAGAA CAACATGCCT
    GGAATCCAGG GAGCCACATC TTCACCTCAA CCACAGGCTA CTTTATTTCA CAACACTACA
    GGAGGTACAA TGAACCAGCT ACAGAATTCT CCTGGCTCTT CTCAACAGAC ATCTGGAATG
    TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CTTCTGGACC AGCTACATTG
    CCAGATCAGT TGATGGCCAT AAGTCCACCA GGCCAGCCAC AAAACGAGGG CCAACCACCT
    GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CAATGGCATC CTCTATAAAC
    ACCAACCAGA ACATTGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC
    TTAACTGGCT CCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28565
    Clone ID Related Accession (Same CDS sequence) XM_002694839.5 , XM_015467392.1
    Accession Version XM_002694839.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694839.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTC CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CAGCTGTTGC TTTGAATGTG AATGTAAAGA AGGAAATATC TAGTCCGGCA
    AGACCTTGCT CTTTCGAAGA AGCCATGAAA GCAATGAAAA CTACTGGATG TAACTTAGAT
    AAGGTAAATA TGCTTCCTAA TGCTCTGATC ACTCCGCTCA TATCAAGCAC TATGATTAAG
    AGTGAAGACA TTACTCCAAT GGAAGTAACA GCAGAGAAAA GATCGCCTTC TATTTTTAAG
    ACTACAAAGA CAGTTGGATC AACTCAACAA ACGTTAGAAA ATCTCTCTCA CATAGCAGGA
    AATGGGTCTT TTTCATCATC ATCTTCCCAC TTAACTTCTG AAAATGAAAA GCAACAACAG
    ATTCAGCCCA AGGCATACAA CCCAGAGACC CTGACAACTA TCCAAACACA GGACATTTCA
    CAGCCTGGTA CTTTTCCAGC GGTTTCTGCG TCTAGTCAGC TGCCCAGCAA TGACGCACTA
    CTACAGCAGG CTACACAGTT TCAAACAAGA GAAACTCAGT CTAGAGAGGT GTTACAGTCA
    GATGGTACAG TGGTTAACTT GTCACACCTG ACTGAGACAT CACAGCAACA GCAGCAGTCT
    CCACTGCAAG AACAAGCACA GACTTTACAG CAGCAGATTT CATCAAATAT TTTCCCATCA
    CCAAATAGTG TGAGTCAGCA ACTACAAAAT ACGATACAAC ACTTGCAGGC AGGGAGTTTT
    ACAGGCAGTA CTGCCAGTGG CAGCAATGGA AATGTTGACT TGGTCCAACA AGTTTTAGAG
    GCACAACAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAACGA GAATGTTCAA
    GAACAGCTTA GTGCAGACAT TTTTCAACAA GTCAGTCAAA TTCAAAATAG CGTAAGCCCT
    GGAATGTTTT CCTCAACAGA ACCAGCAGTC CATACCAGAC CAGATAATTT AATAGCTGGA
    AGAGCTGAAA GTGTTCACCC ACAGAATGAA AACACATTGT CTAATCAGCA ACAGCAACAG
    CAGCAACAGC AAGTGATGGA CTCCTCAGCT GCAATGGTGA TGGAGATGCA ACAGAGTATC
    TGCCAGGCAG CTGCCCAGAT TCAGTCAGAG CTGTTTCCTT CATCTGCTTC AGCAAATGGA
    AACCTTCAGC AGTCTCCAGT TTACCAGCAG ACTTCTCATA TGATGAGCGC ATTATCAGCC
    AATGAGGACA TGCAAATGCA ATGTGAATTG TTTTCTTCTC CTCCGGCAGT TTCTGGAAAT
    GAAACTACTA CAACTACCAC ACAGCAGGTT GCAACTTCTG GCACTACCCT GTTTCAGACA
    TCAAATTCAG GAGATGGAGA AGAGACTGGA GCACAAGCAA AACAGATTCA GAACAGTGTC
    TTTCAGACCA TGGTCCAAAT GCAACATAGT GGGGACAGTC AGCCTCAAGT TGGCCTTTTT
    TCATCTACAA AAAGTATGAT AAGTGTTCAA AATAGTGGTA CCCAGCAGCA AGGCAATGGT
    TTATTCCAGC AAGGTAATGA GATGATGTCA CTTCAATCAG GGAATTTTTT GCAGCAGTCT
    TCTCATTCAC AGGCTCAGCT TTTTCATCCT CAGAATCCTA TTGCTGATCC TCAGAACCTT
    TCCCAGGAAA CTCAAGGTTC TATTTTTCAT AGTCCAAGTC CTATTGTCCA CAGTCAGACT
    TCTACAGCCT CCTCTGAACA AATGCAGCCT CCAATGTTTC ACTCTCAAAA TACCATGGCT
    GTGCTGCAGG GCTCTTCTGT TCCTCAAGAC CAACAGTCAG CCAACATATT TCTTTCCCAA
    AGTCCAATGA ATAATCTCCA AACCAACACA GTGGCCCAGG AAGAACAGAT TTCATTTTTT
    GCAGCTCAGA ATTCAATTTC TCCACTTCAG TCAACATCAA ACACTGAGCA GCAAGCTGCT
    TTCCAGCAGC AGGCTCCAAT ATCACACATT CAGACCCCTA TGCTTTCCCA GGAACAGGCA
    CAACCCTCCC AGCAAGGTCT GTTTCAGCCT CAGGTTTCCC TGGGCTCCCT TCCTCCTAAC
    CCAATGCCTC AAAACCAACA AGGAACAATC TTTCAGTCAC AGCACTCAAT AGTTGCCATC
    CAGAGTAACT CTCCATCCCA GGAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAACAACAG
    AGCATTTTAT TCAGTAACCA GAACGCCATG GCACCAATGG CGTCTCAAAA GCAGCCACCA
    CCAAACATGA TATTCAATCC AAGTCAAAAT CCAGTGGCTA ATCAGGAGCA GCAGAACCAA
    TCAATTTTCC ATCAACAAAA TAATATGGCC CCAATGAATC AAGAGCAGCA GCCCATGCAA
    TTTCAGAACC AGACCACGGT TTCCTCACTT CAGAACCCAG GTCCTGCCCA ATCCGAATCA
    TCACAGACCT CCTTGTTCCA TAGCTCACCT CAGATTCAGT TGGTCCAAGG GTCACCCAGT
    TCTCAAGAGC AGCAAGTAAC ACTCTTCCTC TCTCCAGCAT CCATGTCTGC ATTGCAGACC
    AGTATGAACC AACAAGACAT GCAACAGTCT CCCCTTTATT CCCCTCAGAA CAACATGCCT
    GGAATCCAGG GAGCCACATC TTCACCTCAA CCACAGGCTA CTTTATTTCA CAACACTACA
    GGAGGTACAA TGAACCAGCT ACAGAATTCT CCTGGCTCTT CTCAACAGAC ATCTGGAATG
    TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CTTCTGGACC AGCTACATTG
    CCAGATCAGT TGATGGCCAT AAGTCCACCA GGCCAGCCAC AAAACGAGGG CCAACCACCT
    GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CAATGGCATC CTCTATAAAC
    ACCAACCAGA ACATTGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC
    TTAACTGGCT CCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694885.1
    CDS98..4735
    Translation

    Target ORF information:

    RefSeq Version XM_002694839.5
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694839.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTC CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CAGCTGTTGC TTTGAATGTG AATGTAAAGA AGGAAATATC TAGTCCGGCA
    AGACCTTGCT CTTTCGAAGA AGCCATGAAA GCAATGAAAA CTACTGGATG TAACTTAGAT
    AAGGTAAATA TGCTTCCTAA TGCTCTGATC ACTCCGCTCA TATCAAGCAC TATGATTAAG
    AGTGAAGACA TTACTCCAAT GGAAGTAACA GCAGAGAAAA GATCGCCTTC TATTTTTAAG
    ACTACAAAGA CAGTTGGATC AACTCAACAA ACGTTAGAAA ATCTCTCTCA CATAGCAGGA
    AATGGGTCTT TTTCATCATC ATCTTCCCAC TTAACTTCTG AAAATGAAAA GCAACAACAG
    ATTCAGCCCA AGGCATACAA CCCAGAGACC CTGACAACTA TCCAAACACA GGACATTTCA
    CAGCCTGGTA CTTTTCCAGC GGTTTCTGCG TCTAGTCAGC TGCCCAGCAA TGACGCACTA
    CTACAGCAGG CTACACAGTT TCAAACAAGA GAAACTCAGT CTAGAGAGGT GTTACAGTCA
    GATGGTACAG TGGTTAACTT GTCACACCTG ACTGAGACAT CACAGCAACA GCAGCAGTCT
    CCACTGCAAG AACAAGCACA GACTTTACAG CAGCAGATTT CATCAAATAT TTTCCCATCA
    CCAAATAGTG TGAGTCAGCA ACTACAAAAT ACGATACAAC ACTTGCAGGC AGGGAGTTTT
    ACAGGCAGTA CTGCCAGTGG CAGCAATGGA AATGTTGACT TGGTCCAACA AGTTTTAGAG
    GCACAACAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAACGA GAATGTTCAA
    GAACAGCTTA GTGCAGACAT TTTTCAACAA GTCAGTCAAA TTCAAAATAG CGTAAGCCCT
    GGAATGTTTT CCTCAACAGA ACCAGCAGTC CATACCAGAC CAGATAATTT AATAGCTGGA
    AGAGCTGAAA GTGTTCACCC ACAGAATGAA AACACATTGT CTAATCAGCA ACAGCAACAG
    CAGCAACAGC AAGTGATGGA CTCCTCAGCT GCAATGGTGA TGGAGATGCA ACAGAGTATC
    TGCCAGGCAG CTGCCCAGAT TCAGTCAGAG CTGTTTCCTT CATCTGCTTC AGCAAATGGA
    AACCTTCAGC AGTCTCCAGT TTACCAGCAG ACTTCTCATA TGATGAGCGC ATTATCAGCC
    AATGAGGACA TGCAAATGCA ATGTGAATTG TTTTCTTCTC CTCCGGCAGT TTCTGGAAAT
    GAAACTACTA CAACTACCAC ACAGCAGGTT GCAACTTCTG GCACTACCCT GTTTCAGACA
    TCAAATTCAG GAGATGGAGA AGAGACTGGA GCACAAGCAA AACAGATTCA GAACAGTGTC
    TTTCAGACCA TGGTCCAAAT GCAACATAGT GGGGACAGTC AGCCTCAAGT TGGCCTTTTT
    TCATCTACAA AAAGTATGAT AAGTGTTCAA AATAGTGGTA CCCAGCAGCA AGGCAATGGT
    TTATTCCAGC AAGGTAATGA GATGATGTCA CTTCAATCAG GGAATTTTTT GCAGCAGTCT
    TCTCATTCAC AGGCTCAGCT TTTTCATCCT CAGAATCCTA TTGCTGATCC TCAGAACCTT
    TCCCAGGAAA CTCAAGGTTC TATTTTTCAT AGTCCAAGTC CTATTGTCCA CAGTCAGACT
    TCTACAGCCT CCTCTGAACA AATGCAGCCT CCAATGTTTC ACTCTCAAAA TACCATGGCT
    GTGCTGCAGG GCTCTTCTGT TCCTCAAGAC CAACAGTCAG CCAACATATT TCTTTCCCAA
    AGTCCAATGA ATAATCTCCA AACCAACACA GTGGCCCAGG AAGAACAGAT TTCATTTTTT
    GCAGCTCAGA ATTCAATTTC TCCACTTCAG TCAACATCAA ACACTGAGCA GCAAGCTGCT
    TTCCAGCAGC AGGCTCCAAT ATCACACATT CAGACCCCTA TGCTTTCCCA GGAACAGGCA
    CAACCCTCCC AGCAAGGTCT GTTTCAGCCT CAGGTTTCCC TGGGCTCCCT TCCTCCTAAC
    CCAATGCCTC AAAACCAACA AGGAACAATC TTTCAGTCAC AGCACTCAAT AGTTGCCATC
    CAGAGTAACT CTCCATCCCA GGAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAACAACAG
    AGCATTTTAT TCAGTAACCA GAACGCCATG GCACCAATGG CGTCTCAAAA GCAGCCACCA
    CCAAACATGA TATTCAATCC AAGTCAAAAT CCAGTGGCTA ATCAGGAGCA GCAGAACCAA
    TCAATTTTCC ATCAACAAAA TAATATGGCC CCAATGAATC AAGAGCAGCA GCCCATGCAA
    TTTCAGAACC AGACCACGGT TTCCTCACTT CAGAACCCAG GTCCTGCCCA ATCCGAATCA
    TCACAGACCT CCTTGTTCCA TAGCTCACCT CAGATTCAGT TGGTCCAAGG GTCACCCAGT
    TCTCAAGAGC AGCAAGTAAC ACTCTTCCTC TCTCCAGCAT CCATGTCTGC ATTGCAGACC
    AGTATGAACC AACAAGACAT GCAACAGTCT CCCCTTTATT CCCCTCAGAA CAACATGCCT
    GGAATCCAGG GAGCCACATC TTCACCTCAA CCACAGGCTA CTTTATTTCA CAACACTACA
    GGAGGTACAA TGAACCAGCT ACAGAATTCT CCTGGCTCTT CTCAACAGAC ATCTGGAATG
    TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CTTCTGGACC AGCTACATTG
    CCAGATCAGT TGATGGCCAT AAGTCCACCA GGCCAGCCAC AAAACGAGGG CCAACCACCT
    GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CAATGGCATC CTCTATAAAC
    ACCAACCAGA ACATTGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC
    TTAACTGGCT CCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28565
    Clone ID Related Accession (Same CDS sequence) XM_002694839.5 , XM_015467392.1
    Accession Version XM_015467392.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTC CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CAGCTGTTGC TTTGAATGTG AATGTAAAGA AGGAAATATC TAGTCCGGCA
    AGACCTTGCT CTTTCGAAGA AGCCATGAAA GCAATGAAAA CTACTGGATG TAACTTAGAT
    AAGGTAAATA TGCTTCCTAA TGCTCTGATC ACTCCGCTCA TATCAAGCAC TATGATTAAG
    AGTGAAGACA TTACTCCAAT GGAAGTAACA GCAGAGAAAA GATCGCCTTC TATTTTTAAG
    ACTACAAAGA CAGTTGGATC AACTCAACAA ACGTTAGAAA ATCTCTCTCA CATAGCAGGA
    AATGGGTCTT TTTCATCATC ATCTTCCCAC TTAACTTCTG AAAATGAAAA GCAACAACAG
    ATTCAGCCCA AGGCATACAA CCCAGAGACC CTGACAACTA TCCAAACACA GGACATTTCA
    CAGCCTGGTA CTTTTCCAGC GGTTTCTGCG TCTAGTCAGC TGCCCAGCAA TGACGCACTA
    CTACAGCAGG CTACACAGTT TCAAACAAGA GAAACTCAGT CTAGAGAGGT GTTACAGTCA
    GATGGTACAG TGGTTAACTT GTCACACCTG ACTGAGACAT CACAGCAACA GCAGCAGTCT
    CCACTGCAAG AACAAGCACA GACTTTACAG CAGCAGATTT CATCAAATAT TTTCCCATCA
    CCAAATAGTG TGAGTCAGCA ACTACAAAAT ACGATACAAC ACTTGCAGGC AGGGAGTTTT
    ACAGGCAGTA CTGCCAGTGG CAGCAATGGA AATGTTGACT TGGTCCAACA AGTTTTAGAG
    GCACAACAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAACGA GAATGTTCAA
    GAACAGCTTA GTGCAGACAT TTTTCAACAA GTCAGTCAAA TTCAAAATAG CGTAAGCCCT
    GGAATGTTTT CCTCAACAGA ACCAGCAGTC CATACCAGAC CAGATAATTT AATAGCTGGA
    AGAGCTGAAA GTGTTCACCC ACAGAATGAA AACACATTGT CTAATCAGCA ACAGCAACAG
    CAGCAACAGC AAGTGATGGA CTCCTCAGCT GCAATGGTGA TGGAGATGCA ACAGAGTATC
    TGCCAGGCAG CTGCCCAGAT TCAGTCAGAG CTGTTTCCTT CATCTGCTTC AGCAAATGGA
    AACCTTCAGC AGTCTCCAGT TTACCAGCAG ACTTCTCATA TGATGAGCGC ATTATCAGCC
    AATGAGGACA TGCAAATGCA ATGTGAATTG TTTTCTTCTC CTCCGGCAGT TTCTGGAAAT
    GAAACTACTA CAACTACCAC ACAGCAGGTT GCAACTTCTG GCACTACCCT GTTTCAGACA
    TCAAATTCAG GAGATGGAGA AGAGACTGGA GCACAAGCAA AACAGATTCA GAACAGTGTC
    TTTCAGACCA TGGTCCAAAT GCAACATAGT GGGGACAGTC AGCCTCAAGT TGGCCTTTTT
    TCATCTACAA AAAGTATGAT AAGTGTTCAA AATAGTGGTA CCCAGCAGCA AGGCAATGGT
    TTATTCCAGC AAGGTAATGA GATGATGTCA CTTCAATCAG GGAATTTTTT GCAGCAGTCT
    TCTCATTCAC AGGCTCAGCT TTTTCATCCT CAGAATCCTA TTGCTGATCC TCAGAACCTT
    TCCCAGGAAA CTCAAGGTTC TATTTTTCAT AGTCCAAGTC CTATTGTCCA CAGTCAGACT
    TCTACAGCCT CCTCTGAACA AATGCAGCCT CCAATGTTTC ACTCTCAAAA TACCATGGCT
    GTGCTGCAGG GCTCTTCTGT TCCTCAAGAC CAACAGTCAG CCAACATATT TCTTTCCCAA
    AGTCCAATGA ATAATCTCCA AACCAACACA GTGGCCCAGG AAGAACAGAT TTCATTTTTT
    GCAGCTCAGA ATTCAATTTC TCCACTTCAG TCAACATCAA ACACTGAGCA GCAAGCTGCT
    TTCCAGCAGC AGGCTCCAAT ATCACACATT CAGACCCCTA TGCTTTCCCA GGAACAGGCA
    CAACCCTCCC AGCAAGGTCT GTTTCAGCCT CAGGTTTCCC TGGGCTCCCT TCCTCCTAAC
    CCAATGCCTC AAAACCAACA AGGAACAATC TTTCAGTCAC AGCACTCAAT AGTTGCCATC
    CAGAGTAACT CTCCATCCCA GGAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAACAACAG
    AGCATTTTAT TCAGTAACCA GAACGCCATG GCACCAATGG CGTCTCAAAA GCAGCCACCA
    CCAAACATGA TATTCAATCC AAGTCAAAAT CCAGTGGCTA ATCAGGAGCA GCAGAACCAA
    TCAATTTTCC ATCAACAAAA TAATATGGCC CCAATGAATC AAGAGCAGCA GCCCATGCAA
    TTTCAGAACC AGACCACGGT TTCCTCACTT CAGAACCCAG GTCCTGCCCA ATCCGAATCA
    TCACAGACCT CCTTGTTCCA TAGCTCACCT CAGATTCAGT TGGTCCAAGG GTCACCCAGT
    TCTCAAGAGC AGCAAGTAAC ACTCTTCCTC TCTCCAGCAT CCATGTCTGC ATTGCAGACC
    AGTATGAACC AACAAGACAT GCAACAGTCT CCCCTTTATT CCCCTCAGAA CAACATGCCT
    GGAATCCAGG GAGCCACATC TTCACCTCAA CCACAGGCTA CTTTATTTCA CAACACTACA
    GGAGGTACAA TGAACCAGCT ACAGAATTCT CCTGGCTCTT CTCAACAGAC ATCTGGAATG
    TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CTTCTGGACC AGCTACATTG
    CCAGATCAGT TGATGGCCAT AAGTCCACCA GGCCAGCCAC AAAACGAGGG CCAACCACCT
    GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CAATGGCATC CTCTATAAAC
    ACCAACCAGA ACATTGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC
    TTAACTGGCT CCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322878.1
    CDS98..4735
    Translation

    Target ORF information:

    RefSeq Version XM_015467392.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTC CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CAGCTGTTGC TTTGAATGTG AATGTAAAGA AGGAAATATC TAGTCCGGCA
    AGACCTTGCT CTTTCGAAGA AGCCATGAAA GCAATGAAAA CTACTGGATG TAACTTAGAT
    AAGGTAAATA TGCTTCCTAA TGCTCTGATC ACTCCGCTCA TATCAAGCAC TATGATTAAG
    AGTGAAGACA TTACTCCAAT GGAAGTAACA GCAGAGAAAA GATCGCCTTC TATTTTTAAG
    ACTACAAAGA CAGTTGGATC AACTCAACAA ACGTTAGAAA ATCTCTCTCA CATAGCAGGA
    AATGGGTCTT TTTCATCATC ATCTTCCCAC TTAACTTCTG AAAATGAAAA GCAACAACAG
    ATTCAGCCCA AGGCATACAA CCCAGAGACC CTGACAACTA TCCAAACACA GGACATTTCA
    CAGCCTGGTA CTTTTCCAGC GGTTTCTGCG TCTAGTCAGC TGCCCAGCAA TGACGCACTA
    CTACAGCAGG CTACACAGTT TCAAACAAGA GAAACTCAGT CTAGAGAGGT GTTACAGTCA
    GATGGTACAG TGGTTAACTT GTCACACCTG ACTGAGACAT CACAGCAACA GCAGCAGTCT
    CCACTGCAAG AACAAGCACA GACTTTACAG CAGCAGATTT CATCAAATAT TTTCCCATCA
    CCAAATAGTG TGAGTCAGCA ACTACAAAAT ACGATACAAC ACTTGCAGGC AGGGAGTTTT
    ACAGGCAGTA CTGCCAGTGG CAGCAATGGA AATGTTGACT TGGTCCAACA AGTTTTAGAG
    GCACAACAGC AGTTATCTTC AGTTTTATTT TCTGCTCCAG ATGGTAACGA GAATGTTCAA
    GAACAGCTTA GTGCAGACAT TTTTCAACAA GTCAGTCAAA TTCAAAATAG CGTAAGCCCT
    GGAATGTTTT CCTCAACAGA ACCAGCAGTC CATACCAGAC CAGATAATTT AATAGCTGGA
    AGAGCTGAAA GTGTTCACCC ACAGAATGAA AACACATTGT CTAATCAGCA ACAGCAACAG
    CAGCAACAGC AAGTGATGGA CTCCTCAGCT GCAATGGTGA TGGAGATGCA ACAGAGTATC
    TGCCAGGCAG CTGCCCAGAT TCAGTCAGAG CTGTTTCCTT CATCTGCTTC AGCAAATGGA
    AACCTTCAGC AGTCTCCAGT TTACCAGCAG ACTTCTCATA TGATGAGCGC ATTATCAGCC
    AATGAGGACA TGCAAATGCA ATGTGAATTG TTTTCTTCTC CTCCGGCAGT TTCTGGAAAT
    GAAACTACTA CAACTACCAC ACAGCAGGTT GCAACTTCTG GCACTACCCT GTTTCAGACA
    TCAAATTCAG GAGATGGAGA AGAGACTGGA GCACAAGCAA AACAGATTCA GAACAGTGTC
    TTTCAGACCA TGGTCCAAAT GCAACATAGT GGGGACAGTC AGCCTCAAGT TGGCCTTTTT
    TCATCTACAA AAAGTATGAT AAGTGTTCAA AATAGTGGTA CCCAGCAGCA AGGCAATGGT
    TTATTCCAGC AAGGTAATGA GATGATGTCA CTTCAATCAG GGAATTTTTT GCAGCAGTCT
    TCTCATTCAC AGGCTCAGCT TTTTCATCCT CAGAATCCTA TTGCTGATCC TCAGAACCTT
    TCCCAGGAAA CTCAAGGTTC TATTTTTCAT AGTCCAAGTC CTATTGTCCA CAGTCAGACT
    TCTACAGCCT CCTCTGAACA AATGCAGCCT CCAATGTTTC ACTCTCAAAA TACCATGGCT
    GTGCTGCAGG GCTCTTCTGT TCCTCAAGAC CAACAGTCAG CCAACATATT TCTTTCCCAA
    AGTCCAATGA ATAATCTCCA AACCAACACA GTGGCCCAGG AAGAACAGAT TTCATTTTTT
    GCAGCTCAGA ATTCAATTTC TCCACTTCAG TCAACATCAA ACACTGAGCA GCAAGCTGCT
    TTCCAGCAGC AGGCTCCAAT ATCACACATT CAGACCCCTA TGCTTTCCCA GGAACAGGCA
    CAACCCTCCC AGCAAGGTCT GTTTCAGCCT CAGGTTTCCC TGGGCTCCCT TCCTCCTAAC
    CCAATGCCTC AAAACCAACA AGGAACAATC TTTCAGTCAC AGCACTCAAT AGTTGCCATC
    CAGAGTAACT CTCCATCCCA GGAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAACAACAG
    AGCATTTTAT TCAGTAACCA GAACGCCATG GCACCAATGG CGTCTCAAAA GCAGCCACCA
    CCAAACATGA TATTCAATCC AAGTCAAAAT CCAGTGGCTA ATCAGGAGCA GCAGAACCAA
    TCAATTTTCC ATCAACAAAA TAATATGGCC CCAATGAATC AAGAGCAGCA GCCCATGCAA
    TTTCAGAACC AGACCACGGT TTCCTCACTT CAGAACCCAG GTCCTGCCCA ATCCGAATCA
    TCACAGACCT CCTTGTTCCA TAGCTCACCT CAGATTCAGT TGGTCCAAGG GTCACCCAGT
    TCTCAAGAGC AGCAAGTAAC ACTCTTCCTC TCTCCAGCAT CCATGTCTGC ATTGCAGACC
    AGTATGAACC AACAAGACAT GCAACAGTCT CCCCTTTATT CCCCTCAGAA CAACATGCCT
    GGAATCCAGG GAGCCACATC TTCACCTCAA CCACAGGCTA CTTTATTTCA CAACACTACA
    GGAGGTACAA TGAACCAGCT ACAGAATTCT CCTGGCTCTT CTCAACAGAC ATCTGGAATG
    TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA CTTCTGGACC AGCTACATTG
    CCAGATCAGT TGATGGCCAT AAGTCCACCA GGCCAGCCAC AAAACGAGGG CCAACCACCT
    GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC CAATGGCATC CTCTATAAAC
    ACCAACCAGA ACATTGAAAA GATTGATTTG CTTGTTTCAT TGCAAAACCA AGGGAACAAC
    TTAACTGGCT CCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28566
    Clone ID Related Accession (Same CDS sequence) XM_003587226.4 , XM_015467394.1
    Accession Version XM_003587226.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587226.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT
    AGAGAAACAC CTGTAGCATC AATGAGTCAG ACAAGTGGTG GTGAGGCAGG CTCGCCTCCT
    CCAGCTGTAG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT
    TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC
    AAGGCTATGC AAGTCGAGAG CTGCTCCTCA GCCTTGGGGG TAAGTAACCG AGGGGTAAGT
    GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA
    GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG
    GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC
    TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA
    TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT
    AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT
    ACCACGGACA ACAAAGGCAG CTCAAAAGCA GGAAATGGAA CATTGGAAAA CCAAAAAGGA
    ACTGGAGTAA AGAAGAGCCC TATGTTATGT GGACAGTATC CTGTTAAAAG TGAGGGAAAG
    GAGCTGAAGA TAGTTGTACA ACCTGAGACC CAGCACAGAG CTCGGTACCT GACTGAGGGT
    AGTCGTGGCT CAGTAAAGGA CAGAACACAG CAAGGCTTTC CTACAGTGAA GCTGGAAGGC
    CATAATGAGC CAGTGGTGTT GCAGGTGTTT GTGGGCAATG ACTCTGGTCG AGTGAAACCA
    CATGGATTTT ATCAGGCCTG CAGGGTAACT GGGCGAAATA CAACTCCCTG CAAAGAAGTG
    GATATCGAAG GCACTACTGT TATAGAAGTT GGCCTTGATC CAAGCAACAA TATGACACTG
    GCGGTTGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTTGAAGC CAGAATAGGA
    ATTGCTGGTT CCAAGAAAAA GAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCACA
    AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT ATGTACTCAA
    CCAGCAGGAG TGCCAGAAAT CTTAAAGAAG AGCTTGCATA GCTGTTCGGT GAAAGGAGAG
    GAAGAAGTAT TTTTAATTGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA
    AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ATTATTTCAT
    CAGAATCATC TTATTGTGAA GGTTCCCCCA TATCATGATC AACATATAAC TTTGCCAGTA
    GCAGTGGGAA TATATGTAGT AACCAATGCT GGAAGATCTC ATGATGTTCA GCCTTTCACT
    TACACTCCAG ACCCAGCAGC TGTTGCTTTG AATGTGAATG TAAAGAAGGA AATATCTAGT
    CCGGCAAGAC CTTGCTCTTT CGAAGAAGCC ATGAAAGCAA TGAAAACTAC TGGATGTAAC
    TTAGATAAGG TAAATATGCT TCCTAATGCT CTGATCACTC CGCTCATATC AAGCACTATG
    ATTAAGAGTG AAGACATTAC TCCAATGGAA GTAACAGCAG AGAAAAGATC GCCTTCTATT
    TTTAAGACTA CAAAGACAGT TGGATCAACT CAACAAACGT TAGAAAATCT CTCTCACATA
    GCAGGAAATG GGTCTTTTTC ATCATCATCT TCCCACTTAA CTTCTGAAAA TGAAAAGCAA
    CAACAGATTC AGCCCAAGGC ATACAACCCA GAGACCCTGA CAACTATCCA AACACAGGAC
    ATTTCACAGC CTGGTACTTT TCCAGCGGTT TCTGCGTCTA GTCAGCTGCC CAGCAATGAC
    GCACTACTAC AGCAGGCTAC ACAGTTTCAA ACAAGAGAAA CTCAGTCTAG AGAGGTGTTA
    CAGTCAGATG GTACAGTGGT TAACTTGTCA CACCTGACTG AGACATCACA GCAACAGCAG
    CAGTCTCCAC TGCAAGAACA AGCACAGACT TTACAGCAGC AGATTTCATC AAATATTTTC
    CCATCACCAA ATAGTGTGAG TCAGCAACTA CAAAATACGA TACAACACTT GCAGGCAGGG
    AGTTTTACAG GCAGTACTGC CAGTGGCAGC AATGGAAATG TTGACTTGGT CCAACAAGTT
    TTAGAGGCAC AACAGCAGTT ATCTTCAGTT TTATTTTCTG CTCCAGATGG TAACGAGAAT
    GTTCAAGAAC AGCTTAGTGC AGACATTTTT CAACAAGTCA GTCAAATTCA AAATAGCGTA
    AGCCCTGGAA TGTTTTCCTC AACAGAACCA GCAGTCCATA CCAGACCAGA TAATTTAATA
    GCTGGAAGAG CTGAAAGTGT TCACCCACAG AATGAAAACA CATTGTCTAA TCAGCAACAG
    CAACAGCAGC AACAGCAAGT GATGGACTCC TCAGCTGCAA TGGTGATGGA GATGCAACAG
    AGTATCTGCC AGGCAGCTGC CCAGATTCAG TCAGAGCTGT TTCCTTCATC TGCTTCAGCA
    AATGGAAACC TTCAGCAGTC TCCAGTTTAC CAGCAGACTT CTCATATGAT GAGCGCATTA
    TCAGCCAATG AGGACATGCA AATGCAATGT GAATTGTTTT CTTCTCCTCC GGCAGTTTCT
    GGAAATGAAA CTACTACAAC TACCACACAG CAGGTTGCAA CTTCTGGCAC TACCCTGTTT
    CAGACATCAA ATTCAGGAGA TGGAGAAGAG ACTGGAGCAC AAGCAAAACA GATTCAGAAC
    AGTGTCTTTC AGACCATGGT CCAAATGCAA CATAGTGGGG ACAGTCAGCC TCAAGTTGGC
    CTTTTTTCAT CTACAAAAAG TATGATAAGT GTTCAAAATA GTGGTACCCA GCAGCAAGGC
    AATGGTTTAT TCCAGCAAGG TAATGAGATG ATGTCACTTC AATCAGGGAA TTTTTTGCAG
    CAGTCTTCTC ATTCACAGGC TCAGCTTTTT CATCCTCAGA ATCCTATTGC TGATCCTCAG
    AACCTTTCCC AGGAAACTCA AGGTTCTATT TTTCATAGTC CAAGTCCTAT TGTCCACAGT
    CAGACTTCTA CAGCCTCCTC TGAACAAATG CAGCCTCCAA TGTTTCACTC TCAAAATACC
    ATGGCTGTGC TGCAGGGCTC TTCTGTTCCT CAAGACCAAC AGTCAGCCAA CATATTTCTT
    TCCCAAAGTC CAATGAATAA TCTCCAAACC AACACAGTGG CCCAGGAAGA ACAGATTTCA
    TTTTTTGCAG CTCAGAATTC AATTTCTCCA CTTCAGTCAA CATCAAACAC TGAGCAGCAA
    GCTGCTTTCC AGCAGCAGGC TCCAATATCA CACATTCAGA CCCCTATGCT TTCCCAGGAA
    CAGGCACAAC CCTCCCAGCA AGGTCTGTTT CAGCCTCAGG TTTCCCTGGG CTCCCTTCCT
    CCTAACCCAA TGCCTCAAAA CCAACAAGGA ACAATCTTTC AGTCACAGCA CTCAATAGTT
    GCCATCCAGA GTAACTCTCC ATCCCAGGAG CAGCAGCAGC AGCAACAACA GCAGCAGCAA
    CAACAGAGCA TTTTATTCAG TAACCAGAAC GCCATGGCAC CAATGGCGTC TCAAAAGCAG
    CCACCACCAA ACATGATATT CAATCCAAGT CAAAATCCAG TGGCTAATCA GGAGCAGCAG
    AACCAATCAA TTTTCCATCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC
    ATGCAATTTC AGAACCAGAC CACGGTTTCC TCACTTCAGA ACCCAGGTCC TGCCCAATCC
    GAATCATCAC AGACCTCCTT GTTCCATAGC TCACCTCAGA TTCAGTTGGT CCAAGGGTCA
    CCCAGTTCTC AAGAGCAGCA AGTAACACTC TTCCTCTCTC CAGCATCCAT GTCTGCATTG
    CAGACCAGTA TGAACCAACA AGACATGCAA CAGTCTCCCC TTTATTCCCC TCAGAACAAC
    ATGCCTGGAA TCCAGGGAGC CACATCTTCA CCTCAACCAC AGGCTACTTT ATTTCACAAC
    ACTACAGGAG GTACAATGAA CCAGCTACAG AATTCTCCTG GCTCTTCTCA ACAGACATCT
    GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT
    ACATTGCCAG ATCAGTTGAT GGCCATAAGT CCACCAGGCC AGCCACAAAA CGAGGGCCAA
    CCACCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTCCAAT GGCATCCTCT
    ATAAACACCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG
    AACAACTTAA CTGGCTCCTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587274.1
    CDS98..4681
    Translation

    Target ORF information:

    RefSeq Version XM_003587226.4
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587226.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT
    AGAGAAACAC CTGTAGCATC AATGAGTCAG ACAAGTGGTG GTGAGGCAGG CTCGCCTCCT
    CCAGCTGTAG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT
    TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC
    AAGGCTATGC AAGTCGAGAG CTGCTCCTCA GCCTTGGGGG TAAGTAACCG AGGGGTAAGT
    GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA
    GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG
    GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC
    TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA
    TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT
    AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT
    ACCACGGACA ACAAAGGCAG CTCAAAAGCA GGAAATGGAA CATTGGAAAA CCAAAAAGGA
    ACTGGAGTAA AGAAGAGCCC TATGTTATGT GGACAGTATC CTGTTAAAAG TGAGGGAAAG
    GAGCTGAAGA TAGTTGTACA ACCTGAGACC CAGCACAGAG CTCGGTACCT GACTGAGGGT
    AGTCGTGGCT CAGTAAAGGA CAGAACACAG CAAGGCTTTC CTACAGTGAA GCTGGAAGGC
    CATAATGAGC CAGTGGTGTT GCAGGTGTTT GTGGGCAATG ACTCTGGTCG AGTGAAACCA
    CATGGATTTT ATCAGGCCTG CAGGGTAACT GGGCGAAATA CAACTCCCTG CAAAGAAGTG
    GATATCGAAG GCACTACTGT TATAGAAGTT GGCCTTGATC CAAGCAACAA TATGACACTG
    GCGGTTGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTTGAAGC CAGAATAGGA
    ATTGCTGGTT CCAAGAAAAA GAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCACA
    AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT ATGTACTCAA
    CCAGCAGGAG TGCCAGAAAT CTTAAAGAAG AGCTTGCATA GCTGTTCGGT GAAAGGAGAG
    GAAGAAGTAT TTTTAATTGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA
    AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ATTATTTCAT
    CAGAATCATC TTATTGTGAA GGTTCCCCCA TATCATGATC AACATATAAC TTTGCCAGTA
    GCAGTGGGAA TATATGTAGT AACCAATGCT GGAAGATCTC ATGATGTTCA GCCTTTCACT
    TACACTCCAG ACCCAGCAGC TGTTGCTTTG AATGTGAATG TAAAGAAGGA AATATCTAGT
    CCGGCAAGAC CTTGCTCTTT CGAAGAAGCC ATGAAAGCAA TGAAAACTAC TGGATGTAAC
    TTAGATAAGG TAAATATGCT TCCTAATGCT CTGATCACTC CGCTCATATC AAGCACTATG
    ATTAAGAGTG AAGACATTAC TCCAATGGAA GTAACAGCAG AGAAAAGATC GCCTTCTATT
    TTTAAGACTA CAAAGACAGT TGGATCAACT CAACAAACGT TAGAAAATCT CTCTCACATA
    GCAGGAAATG GGTCTTTTTC ATCATCATCT TCCCACTTAA CTTCTGAAAA TGAAAAGCAA
    CAACAGATTC AGCCCAAGGC ATACAACCCA GAGACCCTGA CAACTATCCA AACACAGGAC
    ATTTCACAGC CTGGTACTTT TCCAGCGGTT TCTGCGTCTA GTCAGCTGCC CAGCAATGAC
    GCACTACTAC AGCAGGCTAC ACAGTTTCAA ACAAGAGAAA CTCAGTCTAG AGAGGTGTTA
    CAGTCAGATG GTACAGTGGT TAACTTGTCA CACCTGACTG AGACATCACA GCAACAGCAG
    CAGTCTCCAC TGCAAGAACA AGCACAGACT TTACAGCAGC AGATTTCATC AAATATTTTC
    CCATCACCAA ATAGTGTGAG TCAGCAACTA CAAAATACGA TACAACACTT GCAGGCAGGG
    AGTTTTACAG GCAGTACTGC CAGTGGCAGC AATGGAAATG TTGACTTGGT CCAACAAGTT
    TTAGAGGCAC AACAGCAGTT ATCTTCAGTT TTATTTTCTG CTCCAGATGG TAACGAGAAT
    GTTCAAGAAC AGCTTAGTGC AGACATTTTT CAACAAGTCA GTCAAATTCA AAATAGCGTA
    AGCCCTGGAA TGTTTTCCTC AACAGAACCA GCAGTCCATA CCAGACCAGA TAATTTAATA
    GCTGGAAGAG CTGAAAGTGT TCACCCACAG AATGAAAACA CATTGTCTAA TCAGCAACAG
    CAACAGCAGC AACAGCAAGT GATGGACTCC TCAGCTGCAA TGGTGATGGA GATGCAACAG
    AGTATCTGCC AGGCAGCTGC CCAGATTCAG TCAGAGCTGT TTCCTTCATC TGCTTCAGCA
    AATGGAAACC TTCAGCAGTC TCCAGTTTAC CAGCAGACTT CTCATATGAT GAGCGCATTA
    TCAGCCAATG AGGACATGCA AATGCAATGT GAATTGTTTT CTTCTCCTCC GGCAGTTTCT
    GGAAATGAAA CTACTACAAC TACCACACAG CAGGTTGCAA CTTCTGGCAC TACCCTGTTT
    CAGACATCAA ATTCAGGAGA TGGAGAAGAG ACTGGAGCAC AAGCAAAACA GATTCAGAAC
    AGTGTCTTTC AGACCATGGT CCAAATGCAA CATAGTGGGG ACAGTCAGCC TCAAGTTGGC
    CTTTTTTCAT CTACAAAAAG TATGATAAGT GTTCAAAATA GTGGTACCCA GCAGCAAGGC
    AATGGTTTAT TCCAGCAAGG TAATGAGATG ATGTCACTTC AATCAGGGAA TTTTTTGCAG
    CAGTCTTCTC ATTCACAGGC TCAGCTTTTT CATCCTCAGA ATCCTATTGC TGATCCTCAG
    AACCTTTCCC AGGAAACTCA AGGTTCTATT TTTCATAGTC CAAGTCCTAT TGTCCACAGT
    CAGACTTCTA CAGCCTCCTC TGAACAAATG CAGCCTCCAA TGTTTCACTC TCAAAATACC
    ATGGCTGTGC TGCAGGGCTC TTCTGTTCCT CAAGACCAAC AGTCAGCCAA CATATTTCTT
    TCCCAAAGTC CAATGAATAA TCTCCAAACC AACACAGTGG CCCAGGAAGA ACAGATTTCA
    TTTTTTGCAG CTCAGAATTC AATTTCTCCA CTTCAGTCAA CATCAAACAC TGAGCAGCAA
    GCTGCTTTCC AGCAGCAGGC TCCAATATCA CACATTCAGA CCCCTATGCT TTCCCAGGAA
    CAGGCACAAC CCTCCCAGCA AGGTCTGTTT CAGCCTCAGG TTTCCCTGGG CTCCCTTCCT
    CCTAACCCAA TGCCTCAAAA CCAACAAGGA ACAATCTTTC AGTCACAGCA CTCAATAGTT
    GCCATCCAGA GTAACTCTCC ATCCCAGGAG CAGCAGCAGC AGCAACAACA GCAGCAGCAA
    CAACAGAGCA TTTTATTCAG TAACCAGAAC GCCATGGCAC CAATGGCGTC TCAAAAGCAG
    CCACCACCAA ACATGATATT CAATCCAAGT CAAAATCCAG TGGCTAATCA GGAGCAGCAG
    AACCAATCAA TTTTCCATCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC
    ATGCAATTTC AGAACCAGAC CACGGTTTCC TCACTTCAGA ACCCAGGTCC TGCCCAATCC
    GAATCATCAC AGACCTCCTT GTTCCATAGC TCACCTCAGA TTCAGTTGGT CCAAGGGTCA
    CCCAGTTCTC AAGAGCAGCA AGTAACACTC TTCCTCTCTC CAGCATCCAT GTCTGCATTG
    CAGACCAGTA TGAACCAACA AGACATGCAA CAGTCTCCCC TTTATTCCCC TCAGAACAAC
    ATGCCTGGAA TCCAGGGAGC CACATCTTCA CCTCAACCAC AGGCTACTTT ATTTCACAAC
    ACTACAGGAG GTACAATGAA CCAGCTACAG AATTCTCCTG GCTCTTCTCA ACAGACATCT
    GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT
    ACATTGCCAG ATCAGTTGAT GGCCATAAGT CCACCAGGCC AGCCACAAAA CGAGGGCCAA
    CCACCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTCCAAT GGCATCCTCT
    ATAAACACCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG
    AACAACTTAA CTGGCTCCTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28566
    Clone ID Related Accession (Same CDS sequence) XM_003587226.4 , XM_015467394.1
    Accession Version XM_015467394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT
    AGAGAAACAC CTGTAGCATC AATGAGTCAG ACAAGTGGTG GTGAGGCAGG CTCGCCTCCT
    CCAGCTGTAG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT
    TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC
    AAGGCTATGC AAGTCGAGAG CTGCTCCTCA GCCTTGGGGG TAAGTAACCG AGGGGTAAGT
    GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA
    GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG
    GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC
    TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA
    TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT
    AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT
    ACCACGGACA ACAAAGGCAG CTCAAAAGCA GGAAATGGAA CATTGGAAAA CCAAAAAGGA
    ACTGGAGTAA AGAAGAGCCC TATGTTATGT GGACAGTATC CTGTTAAAAG TGAGGGAAAG
    GAGCTGAAGA TAGTTGTACA ACCTGAGACC CAGCACAGAG CTCGGTACCT GACTGAGGGT
    AGTCGTGGCT CAGTAAAGGA CAGAACACAG CAAGGCTTTC CTACAGTGAA GCTGGAAGGC
    CATAATGAGC CAGTGGTGTT GCAGGTGTTT GTGGGCAATG ACTCTGGTCG AGTGAAACCA
    CATGGATTTT ATCAGGCCTG CAGGGTAACT GGGCGAAATA CAACTCCCTG CAAAGAAGTG
    GATATCGAAG GCACTACTGT TATAGAAGTT GGCCTTGATC CAAGCAACAA TATGACACTG
    GCGGTTGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTTGAAGC CAGAATAGGA
    ATTGCTGGTT CCAAGAAAAA GAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCACA
    AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT ATGTACTCAA
    CCAGCAGGAG TGCCAGAAAT CTTAAAGAAG AGCTTGCATA GCTGTTCGGT GAAAGGAGAG
    GAAGAAGTAT TTTTAATTGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA
    AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ATTATTTCAT
    CAGAATCATC TTATTGTGAA GGTTCCCCCA TATCATGATC AACATATAAC TTTGCCAGTA
    GCAGTGGGAA TATATGTAGT AACCAATGCT GGAAGATCTC ATGATGTTCA GCCTTTCACT
    TACACTCCAG ACCCAGCAGC TGTTGCTTTG AATGTGAATG TAAAGAAGGA AATATCTAGT
    CCGGCAAGAC CTTGCTCTTT CGAAGAAGCC ATGAAAGCAA TGAAAACTAC TGGATGTAAC
    TTAGATAAGG TAAATATGCT TCCTAATGCT CTGATCACTC CGCTCATATC AAGCACTATG
    ATTAAGAGTG AAGACATTAC TCCAATGGAA GTAACAGCAG AGAAAAGATC GCCTTCTATT
    TTTAAGACTA CAAAGACAGT TGGATCAACT CAACAAACGT TAGAAAATCT CTCTCACATA
    GCAGGAAATG GGTCTTTTTC ATCATCATCT TCCCACTTAA CTTCTGAAAA TGAAAAGCAA
    CAACAGATTC AGCCCAAGGC ATACAACCCA GAGACCCTGA CAACTATCCA AACACAGGAC
    ATTTCACAGC CTGGTACTTT TCCAGCGGTT TCTGCGTCTA GTCAGCTGCC CAGCAATGAC
    GCACTACTAC AGCAGGCTAC ACAGTTTCAA ACAAGAGAAA CTCAGTCTAG AGAGGTGTTA
    CAGTCAGATG GTACAGTGGT TAACTTGTCA CACCTGACTG AGACATCACA GCAACAGCAG
    CAGTCTCCAC TGCAAGAACA AGCACAGACT TTACAGCAGC AGATTTCATC AAATATTTTC
    CCATCACCAA ATAGTGTGAG TCAGCAACTA CAAAATACGA TACAACACTT GCAGGCAGGG
    AGTTTTACAG GCAGTACTGC CAGTGGCAGC AATGGAAATG TTGACTTGGT CCAACAAGTT
    TTAGAGGCAC AACAGCAGTT ATCTTCAGTT TTATTTTCTG CTCCAGATGG TAACGAGAAT
    GTTCAAGAAC AGCTTAGTGC AGACATTTTT CAACAAGTCA GTCAAATTCA AAATAGCGTA
    AGCCCTGGAA TGTTTTCCTC AACAGAACCA GCAGTCCATA CCAGACCAGA TAATTTAATA
    GCTGGAAGAG CTGAAAGTGT TCACCCACAG AATGAAAACA CATTGTCTAA TCAGCAACAG
    CAACAGCAGC AACAGCAAGT GATGGACTCC TCAGCTGCAA TGGTGATGGA GATGCAACAG
    AGTATCTGCC AGGCAGCTGC CCAGATTCAG TCAGAGCTGT TTCCTTCATC TGCTTCAGCA
    AATGGAAACC TTCAGCAGTC TCCAGTTTAC CAGCAGACTT CTCATATGAT GAGCGCATTA
    TCAGCCAATG AGGACATGCA AATGCAATGT GAATTGTTTT CTTCTCCTCC GGCAGTTTCT
    GGAAATGAAA CTACTACAAC TACCACACAG CAGGTTGCAA CTTCTGGCAC TACCCTGTTT
    CAGACATCAA ATTCAGGAGA TGGAGAAGAG ACTGGAGCAC AAGCAAAACA GATTCAGAAC
    AGTGTCTTTC AGACCATGGT CCAAATGCAA CATAGTGGGG ACAGTCAGCC TCAAGTTGGC
    CTTTTTTCAT CTACAAAAAG TATGATAAGT GTTCAAAATA GTGGTACCCA GCAGCAAGGC
    AATGGTTTAT TCCAGCAAGG TAATGAGATG ATGTCACTTC AATCAGGGAA TTTTTTGCAG
    CAGTCTTCTC ATTCACAGGC TCAGCTTTTT CATCCTCAGA ATCCTATTGC TGATCCTCAG
    AACCTTTCCC AGGAAACTCA AGGTTCTATT TTTCATAGTC CAAGTCCTAT TGTCCACAGT
    CAGACTTCTA CAGCCTCCTC TGAACAAATG CAGCCTCCAA TGTTTCACTC TCAAAATACC
    ATGGCTGTGC TGCAGGGCTC TTCTGTTCCT CAAGACCAAC AGTCAGCCAA CATATTTCTT
    TCCCAAAGTC CAATGAATAA TCTCCAAACC AACACAGTGG CCCAGGAAGA ACAGATTTCA
    TTTTTTGCAG CTCAGAATTC AATTTCTCCA CTTCAGTCAA CATCAAACAC TGAGCAGCAA
    GCTGCTTTCC AGCAGCAGGC TCCAATATCA CACATTCAGA CCCCTATGCT TTCCCAGGAA
    CAGGCACAAC CCTCCCAGCA AGGTCTGTTT CAGCCTCAGG TTTCCCTGGG CTCCCTTCCT
    CCTAACCCAA TGCCTCAAAA CCAACAAGGA ACAATCTTTC AGTCACAGCA CTCAATAGTT
    GCCATCCAGA GTAACTCTCC ATCCCAGGAG CAGCAGCAGC AGCAACAACA GCAGCAGCAA
    CAACAGAGCA TTTTATTCAG TAACCAGAAC GCCATGGCAC CAATGGCGTC TCAAAAGCAG
    CCACCACCAA ACATGATATT CAATCCAAGT CAAAATCCAG TGGCTAATCA GGAGCAGCAG
    AACCAATCAA TTTTCCATCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC
    ATGCAATTTC AGAACCAGAC CACGGTTTCC TCACTTCAGA ACCCAGGTCC TGCCCAATCC
    GAATCATCAC AGACCTCCTT GTTCCATAGC TCACCTCAGA TTCAGTTGGT CCAAGGGTCA
    CCCAGTTCTC AAGAGCAGCA AGTAACACTC TTCCTCTCTC CAGCATCCAT GTCTGCATTG
    CAGACCAGTA TGAACCAACA AGACATGCAA CAGTCTCCCC TTTATTCCCC TCAGAACAAC
    ATGCCTGGAA TCCAGGGAGC CACATCTTCA CCTCAACCAC AGGCTACTTT ATTTCACAAC
    ACTACAGGAG GTACAATGAA CCAGCTACAG AATTCTCCTG GCTCTTCTCA ACAGACATCT
    GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT
    ACATTGCCAG ATCAGTTGAT GGCCATAAGT CCACCAGGCC AGCCACAAAA CGAGGGCCAA
    CCACCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTCCAAT GGCATCCTCT
    ATAAACACCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG
    AACAACTTAA CTGGCTCCTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322880.1
    CDS98..4681
    Translation

    Target ORF information:

    RefSeq Version XM_015467394.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGAATCTGT CTATGATCTT CTCCCAAAGG AGTTACAGTT ACCTCCATCT
    AGAGAAACAC CTGTAGCATC AATGAGTCAG ACAAGTGGTG GTGAGGCAGG CTCGCCTCCT
    CCAGCTGTAG TTGCTGCTGA TGCTTCTTCA GCTCCCTCCT CTTCCTCCAT GGGCGGTGCT
    TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT CTACCTCAGT CACCGACAGC
    AAGGCTATGC AAGTCGAGAG CTGCTCCTCA GCCTTGGGGG TAAGTAACCG AGGGGTAAGT
    GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT CAACACCGAA GAGGCACACA
    GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA ACAGTCGGAT GTCCTGCCAG
    GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA TGTGGATGGA GGATTCCCCC
    TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA ACACTGAGGT ACCTCGTAAA
    TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC GACGAGATTG TGAAGAATCT
    AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC ACTATGTGCT TTCTCAGCTT
    ACCACGGACA ACAAAGGCAG CTCAAAAGCA GGAAATGGAA CATTGGAAAA CCAAAAAGGA
    ACTGGAGTAA AGAAGAGCCC TATGTTATGT GGACAGTATC CTGTTAAAAG TGAGGGAAAG
    GAGCTGAAGA TAGTTGTACA ACCTGAGACC CAGCACAGAG CTCGGTACCT GACTGAGGGT
    AGTCGTGGCT CAGTAAAGGA CAGAACACAG CAAGGCTTTC CTACAGTGAA GCTGGAAGGC
    CATAATGAGC CAGTGGTGTT GCAGGTGTTT GTGGGCAATG ACTCTGGTCG AGTGAAACCA
    CATGGATTTT ATCAGGCCTG CAGGGTAACT GGGCGAAATA CAACTCCCTG CAAAGAAGTG
    GATATCGAAG GCACTACTGT TATAGAAGTT GGCCTTGATC CAAGCAACAA TATGACACTG
    GCGGTTGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG ATGTTGAAGC CAGAATAGGA
    ATTGCTGGTT CCAAGAAAAA GAGCACTCGT GCCAGATTGG TTTTTCGAGT TAATATCACA
    AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT CTCCAATTTT ATGTACTCAA
    CCAGCAGGAG TGCCAGAAAT CTTAAAGAAG AGCTTGCATA GCTGTTCGGT GAAAGGAGAG
    GAAGAAGTAT TTTTAATTGG CAAGAACTTT CTGAAAGGAA CTAAAGTTAT TTTCCAAGAA
    AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA TTGATATGGA ATTATTTCAT
    CAGAATCATC TTATTGTGAA GGTTCCCCCA TATCATGATC AACATATAAC TTTGCCAGTA
    GCAGTGGGAA TATATGTAGT AACCAATGCT GGAAGATCTC ATGATGTTCA GCCTTTCACT
    TACACTCCAG ACCCAGCAGC TGTTGCTTTG AATGTGAATG TAAAGAAGGA AATATCTAGT
    CCGGCAAGAC CTTGCTCTTT CGAAGAAGCC ATGAAAGCAA TGAAAACTAC TGGATGTAAC
    TTAGATAAGG TAAATATGCT TCCTAATGCT CTGATCACTC CGCTCATATC AAGCACTATG
    ATTAAGAGTG AAGACATTAC TCCAATGGAA GTAACAGCAG AGAAAAGATC GCCTTCTATT
    TTTAAGACTA CAAAGACAGT TGGATCAACT CAACAAACGT TAGAAAATCT CTCTCACATA
    GCAGGAAATG GGTCTTTTTC ATCATCATCT TCCCACTTAA CTTCTGAAAA TGAAAAGCAA
    CAACAGATTC AGCCCAAGGC ATACAACCCA GAGACCCTGA CAACTATCCA AACACAGGAC
    ATTTCACAGC CTGGTACTTT TCCAGCGGTT TCTGCGTCTA GTCAGCTGCC CAGCAATGAC
    GCACTACTAC AGCAGGCTAC ACAGTTTCAA ACAAGAGAAA CTCAGTCTAG AGAGGTGTTA
    CAGTCAGATG GTACAGTGGT TAACTTGTCA CACCTGACTG AGACATCACA GCAACAGCAG
    CAGTCTCCAC TGCAAGAACA AGCACAGACT TTACAGCAGC AGATTTCATC AAATATTTTC
    CCATCACCAA ATAGTGTGAG TCAGCAACTA CAAAATACGA TACAACACTT GCAGGCAGGG
    AGTTTTACAG GCAGTACTGC CAGTGGCAGC AATGGAAATG TTGACTTGGT CCAACAAGTT
    TTAGAGGCAC AACAGCAGTT ATCTTCAGTT TTATTTTCTG CTCCAGATGG TAACGAGAAT
    GTTCAAGAAC AGCTTAGTGC AGACATTTTT CAACAAGTCA GTCAAATTCA AAATAGCGTA
    AGCCCTGGAA TGTTTTCCTC AACAGAACCA GCAGTCCATA CCAGACCAGA TAATTTAATA
    GCTGGAAGAG CTGAAAGTGT TCACCCACAG AATGAAAACA CATTGTCTAA TCAGCAACAG
    CAACAGCAGC AACAGCAAGT GATGGACTCC TCAGCTGCAA TGGTGATGGA GATGCAACAG
    AGTATCTGCC AGGCAGCTGC CCAGATTCAG TCAGAGCTGT TTCCTTCATC TGCTTCAGCA
    AATGGAAACC TTCAGCAGTC TCCAGTTTAC CAGCAGACTT CTCATATGAT GAGCGCATTA
    TCAGCCAATG AGGACATGCA AATGCAATGT GAATTGTTTT CTTCTCCTCC GGCAGTTTCT
    GGAAATGAAA CTACTACAAC TACCACACAG CAGGTTGCAA CTTCTGGCAC TACCCTGTTT
    CAGACATCAA ATTCAGGAGA TGGAGAAGAG ACTGGAGCAC AAGCAAAACA GATTCAGAAC
    AGTGTCTTTC AGACCATGGT CCAAATGCAA CATAGTGGGG ACAGTCAGCC TCAAGTTGGC
    CTTTTTTCAT CTACAAAAAG TATGATAAGT GTTCAAAATA GTGGTACCCA GCAGCAAGGC
    AATGGTTTAT TCCAGCAAGG TAATGAGATG ATGTCACTTC AATCAGGGAA TTTTTTGCAG
    CAGTCTTCTC ATTCACAGGC TCAGCTTTTT CATCCTCAGA ATCCTATTGC TGATCCTCAG
    AACCTTTCCC AGGAAACTCA AGGTTCTATT TTTCATAGTC CAAGTCCTAT TGTCCACAGT
    CAGACTTCTA CAGCCTCCTC TGAACAAATG CAGCCTCCAA TGTTTCACTC TCAAAATACC
    ATGGCTGTGC TGCAGGGCTC TTCTGTTCCT CAAGACCAAC AGTCAGCCAA CATATTTCTT
    TCCCAAAGTC CAATGAATAA TCTCCAAACC AACACAGTGG CCCAGGAAGA ACAGATTTCA
    TTTTTTGCAG CTCAGAATTC AATTTCTCCA CTTCAGTCAA CATCAAACAC TGAGCAGCAA
    GCTGCTTTCC AGCAGCAGGC TCCAATATCA CACATTCAGA CCCCTATGCT TTCCCAGGAA
    CAGGCACAAC CCTCCCAGCA AGGTCTGTTT CAGCCTCAGG TTTCCCTGGG CTCCCTTCCT
    CCTAACCCAA TGCCTCAAAA CCAACAAGGA ACAATCTTTC AGTCACAGCA CTCAATAGTT
    GCCATCCAGA GTAACTCTCC ATCCCAGGAG CAGCAGCAGC AGCAACAACA GCAGCAGCAA
    CAACAGAGCA TTTTATTCAG TAACCAGAAC GCCATGGCAC CAATGGCGTC TCAAAAGCAG
    CCACCACCAA ACATGATATT CAATCCAAGT CAAAATCCAG TGGCTAATCA GGAGCAGCAG
    AACCAATCAA TTTTCCATCA ACAAAATAAT ATGGCCCCAA TGAATCAAGA GCAGCAGCCC
    ATGCAATTTC AGAACCAGAC CACGGTTTCC TCACTTCAGA ACCCAGGTCC TGCCCAATCC
    GAATCATCAC AGACCTCCTT GTTCCATAGC TCACCTCAGA TTCAGTTGGT CCAAGGGTCA
    CCCAGTTCTC AAGAGCAGCA AGTAACACTC TTCCTCTCTC CAGCATCCAT GTCTGCATTG
    CAGACCAGTA TGAACCAACA AGACATGCAA CAGTCTCCCC TTTATTCCCC TCAGAACAAC
    ATGCCTGGAA TCCAGGGAGC CACATCTTCA CCTCAACCAC AGGCTACTTT ATTTCACAAC
    ACTACAGGAG GTACAATGAA CCAGCTACAG AATTCTCCTG GCTCTTCTCA ACAGACATCT
    GGAATGTTCT TATTTGGCAT TCAAAATAAC TGTAGTCAGC TTTTAACTTC TGGACCAGCT
    ACATTGCCAG ATCAGTTGAT GGCCATAAGT CCACCAGGCC AGCCACAAAA CGAGGGCCAA
    CCACCTGTGA CAACACTTCT TTCTCAGCAA ATGCCAGAGA ATTCTCCAAT GGCATCCTCT
    ATAAACACCA ACCAGAACAT TGAAAAGATT GATTTGCTTG TTTCATTGCA AAACCAAGGG
    AACAACTTAA CTGGCTCCTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53918
    Clone ID Related Accession (Same CDS sequence) XM_010814692.2 , XM_015467393.1
    Accession Version XM_010814692.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTC CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CTGTTGCTTT GAATGTGAAT GTAAAGAAGG AAATATCTAG TCCGGCAAGA
    CCTTGCTCTT TCGAAGAAGC CATGAAAGCA ATGAAAACTA CTGGATGTAA CTTAGATAAG
    GTAAATATGC TTCCTAATGC TCTGATCACT CCGCTCATAT CAAGCACTAT GATTAAGAGT
    GAAGACATTA CTCCAATGGA AGTAACAGCA GAGAAAAGAT CGCCTTCTAT TTTTAAGACT
    ACAAAGACAG TTGGATCAAC TCAACAAACG TTAGAAAATC TCTCTCACAT AGCAGGAAAT
    GGGTCTTTTT CATCATCATC TTCCCACTTA ACTTCTGAAA ATGAAAAGCA ACAACAGATT
    CAGCCCAAGG CATACAACCC AGAGACCCTG ACAACTATCC AAACACAGGA CATTTCACAG
    CCTGGTACTT TTCCAGCGGT TTCTGCGTCT AGTCAGCTGC CCAGCAATGA CGCACTACTA
    CAGCAGGCTA CACAGTTTCA AACAAGAGAA ACTCAGTCTA GAGAGGTGTT ACAGTCAGAT
    GGTACAGTGG TTAACTTGTC ACACCTGACT GAGACATCAC AGCAACAGCA GCAGTCTCCA
    CTGCAAGAAC AAGCACAGAC TTTACAGCAG CAGATTTCAT CAAATATTTT CCCATCACCA
    AATAGTGTGA GTCAGCAACT ACAAAATACG ATACAACACT TGCAGGCAGG GAGTTTTACA
    GGCAGTACTG CCAGTGGCAG CAATGGAAAT GTTGACTTGG TCCAACAAGT TTTAGAGGCA
    CAACAGCAGT TATCTTCAGT TTTATTTTCT GCTCCAGATG GTAACGAGAA TGTTCAAGAA
    CAGCTTAGTG CAGACATTTT TCAACAAGTC AGTCAAATTC AAAATAGCGT AAGCCCTGGA
    ATGTTTTCCT CAACAGAACC AGCAGTCCAT ACCAGACCAG ATAATTTAAT AGCTGGAAGA
    GCTGAAAGTG TTCACCCACA GAATGAAAAC ACATTGTCTA ATCAGCAACA GCAACAGCAG
    CAACAGCAAG TGATGGACTC CTCAGCTGCA ATGGTGATGG AGATGCAACA GAGTATCTGC
    CAGGCAGCTG CCCAGATTCA GTCAGAGCTG TTTCCTTCAT CTGCTTCAGC AAATGGAAAC
    CTTCAGCAGT CTCCAGTTTA CCAGCAGACT TCTCATATGA TGAGCGCATT ATCAGCCAAT
    GAGGACATGC AAATGCAATG TGAATTGTTT TCTTCTCCTC CGGCAGTTTC TGGAAATGAA
    ACTACTACAA CTACCACACA GCAGGTTGCA ACTTCTGGCA CTACCCTGTT TCAGACATCA
    AATTCAGGAG ATGGAGAAGA GACTGGAGCA CAAGCAAAAC AGATTCAGAA CAGTGTCTTT
    CAGACCATGG TCCAAATGCA ACATAGTGGG GACAGTCAGC CTCAAGTTGG CCTTTTTTCA
    TCTACAAAAA GTATGATAAG TGTTCAAAAT AGTGGTACCC AGCAGCAAGG CAATGGTTTA
    TTCCAGCAAG GTAATGAGAT GATGTCACTT CAATCAGGGA ATTTTTTGCA GCAGTCTTCT
    CATTCACAGG CTCAGCTTTT TCATCCTCAG AATCCTATTG CTGATCCTCA GAACCTTTCC
    CAGGAAACTC AAGGTTCTAT TTTTCATAGT CCAAGTCCTA TTGTCCACAG TCAGACTTCT
    ACAGCCTCCT CTGAACAAAT GCAGCCTCCA ATGTTTCACT CTCAAAATAC CATGGCTGTG
    CTGCAGGGCT CTTCTGTTCC TCAAGACCAA CAGTCAGCCA ACATATTTCT TTCCCAAAGT
    CCAATGAATA ATCTCCAAAC CAACACAGTG GCCCAGGAAG AACAGATTTC ATTTTTTGCA
    GCTCAGAATT CAATTTCTCC ACTTCAGTCA ACATCAAACA CTGAGCAGCA AGCTGCTTTC
    CAGCAGCAGG CTCCAATATC ACACATTCAG ACCCCTATGC TTTCCCAGGA ACAGGCACAA
    CCCTCCCAGC AAGGTCTGTT TCAGCCTCAG GTTTCCCTGG GCTCCCTTCC TCCTAACCCA
    ATGCCTCAAA ACCAACAAGG AACAATCTTT CAGTCACAGC ACTCAATAGT TGCCATCCAG
    AGTAACTCTC CATCCCAGGA GCAGCAGCAG CAGCAACAAC AGCAGCAGCA ACAACAGAGC
    ATTTTATTCA GTAACCAGAA CGCCATGGCA CCAATGGCGT CTCAAAAGCA GCCACCACCA
    AACATGATAT TCAATCCAAG TCAAAATCCA GTGGCTAATC AGGAGCAGCA GAACCAATCA
    ATTTTCCATC AACAAAATAA TATGGCCCCA ATGAATCAAG AGCAGCAGCC CATGCAATTT
    CAGAACCAGA CCACGGTTTC CTCACTTCAG AACCCAGGTC CTGCCCAATC CGAATCATCA
    CAGACCTCCT TGTTCCATAG CTCACCTCAG ATTCAGTTGG TCCAAGGGTC ACCCAGTTCT
    CAAGAGCAGC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAGACCAGT
    ATGAACCAAC AAGACATGCA ACAGTCTCCC CTTTATTCCC CTCAGAACAA CATGCCTGGA
    ATCCAGGGAG CCACATCTTC ACCTCAACCA CAGGCTACTT TATTTCACAA CACTACAGGA
    GGTACAATGA ACCAGCTACA GAATTCTCCT GGCTCTTCTC AACAGACATC TGGAATGTTC
    TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACTT CTGGACCAGC TACATTGCCA
    GATCAGTTGA TGGCCATAAG TCCACCAGGC CAGCCACAAA ACGAGGGCCA ACCACCTGTG
    ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAA TGGCATCCTC TATAAACACC
    AACCAGAACA TTGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTA
    ACTGGCTCCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812994.1
    CDS98..4732
    Translation

    Target ORF information:

    RefSeq Version XM_010814692.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTC CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CTGTTGCTTT GAATGTGAAT GTAAAGAAGG AAATATCTAG TCCGGCAAGA
    CCTTGCTCTT TCGAAGAAGC CATGAAAGCA ATGAAAACTA CTGGATGTAA CTTAGATAAG
    GTAAATATGC TTCCTAATGC TCTGATCACT CCGCTCATAT CAAGCACTAT GATTAAGAGT
    GAAGACATTA CTCCAATGGA AGTAACAGCA GAGAAAAGAT CGCCTTCTAT TTTTAAGACT
    ACAAAGACAG TTGGATCAAC TCAACAAACG TTAGAAAATC TCTCTCACAT AGCAGGAAAT
    GGGTCTTTTT CATCATCATC TTCCCACTTA ACTTCTGAAA ATGAAAAGCA ACAACAGATT
    CAGCCCAAGG CATACAACCC AGAGACCCTG ACAACTATCC AAACACAGGA CATTTCACAG
    CCTGGTACTT TTCCAGCGGT TTCTGCGTCT AGTCAGCTGC CCAGCAATGA CGCACTACTA
    CAGCAGGCTA CACAGTTTCA AACAAGAGAA ACTCAGTCTA GAGAGGTGTT ACAGTCAGAT
    GGTACAGTGG TTAACTTGTC ACACCTGACT GAGACATCAC AGCAACAGCA GCAGTCTCCA
    CTGCAAGAAC AAGCACAGAC TTTACAGCAG CAGATTTCAT CAAATATTTT CCCATCACCA
    AATAGTGTGA GTCAGCAACT ACAAAATACG ATACAACACT TGCAGGCAGG GAGTTTTACA
    GGCAGTACTG CCAGTGGCAG CAATGGAAAT GTTGACTTGG TCCAACAAGT TTTAGAGGCA
    CAACAGCAGT TATCTTCAGT TTTATTTTCT GCTCCAGATG GTAACGAGAA TGTTCAAGAA
    CAGCTTAGTG CAGACATTTT TCAACAAGTC AGTCAAATTC AAAATAGCGT AAGCCCTGGA
    ATGTTTTCCT CAACAGAACC AGCAGTCCAT ACCAGACCAG ATAATTTAAT AGCTGGAAGA
    GCTGAAAGTG TTCACCCACA GAATGAAAAC ACATTGTCTA ATCAGCAACA GCAACAGCAG
    CAACAGCAAG TGATGGACTC CTCAGCTGCA ATGGTGATGG AGATGCAACA GAGTATCTGC
    CAGGCAGCTG CCCAGATTCA GTCAGAGCTG TTTCCTTCAT CTGCTTCAGC AAATGGAAAC
    CTTCAGCAGT CTCCAGTTTA CCAGCAGACT TCTCATATGA TGAGCGCATT ATCAGCCAAT
    GAGGACATGC AAATGCAATG TGAATTGTTT TCTTCTCCTC CGGCAGTTTC TGGAAATGAA
    ACTACTACAA CTACCACACA GCAGGTTGCA ACTTCTGGCA CTACCCTGTT TCAGACATCA
    AATTCAGGAG ATGGAGAAGA GACTGGAGCA CAAGCAAAAC AGATTCAGAA CAGTGTCTTT
    CAGACCATGG TCCAAATGCA ACATAGTGGG GACAGTCAGC CTCAAGTTGG CCTTTTTTCA
    TCTACAAAAA GTATGATAAG TGTTCAAAAT AGTGGTACCC AGCAGCAAGG CAATGGTTTA
    TTCCAGCAAG GTAATGAGAT GATGTCACTT CAATCAGGGA ATTTTTTGCA GCAGTCTTCT
    CATTCACAGG CTCAGCTTTT TCATCCTCAG AATCCTATTG CTGATCCTCA GAACCTTTCC
    CAGGAAACTC AAGGTTCTAT TTTTCATAGT CCAAGTCCTA TTGTCCACAG TCAGACTTCT
    ACAGCCTCCT CTGAACAAAT GCAGCCTCCA ATGTTTCACT CTCAAAATAC CATGGCTGTG
    CTGCAGGGCT CTTCTGTTCC TCAAGACCAA CAGTCAGCCA ACATATTTCT TTCCCAAAGT
    CCAATGAATA ATCTCCAAAC CAACACAGTG GCCCAGGAAG AACAGATTTC ATTTTTTGCA
    GCTCAGAATT CAATTTCTCC ACTTCAGTCA ACATCAAACA CTGAGCAGCA AGCTGCTTTC
    CAGCAGCAGG CTCCAATATC ACACATTCAG ACCCCTATGC TTTCCCAGGA ACAGGCACAA
    CCCTCCCAGC AAGGTCTGTT TCAGCCTCAG GTTTCCCTGG GCTCCCTTCC TCCTAACCCA
    ATGCCTCAAA ACCAACAAGG AACAATCTTT CAGTCACAGC ACTCAATAGT TGCCATCCAG
    AGTAACTCTC CATCCCAGGA GCAGCAGCAG CAGCAACAAC AGCAGCAGCA ACAACAGAGC
    ATTTTATTCA GTAACCAGAA CGCCATGGCA CCAATGGCGT CTCAAAAGCA GCCACCACCA
    AACATGATAT TCAATCCAAG TCAAAATCCA GTGGCTAATC AGGAGCAGCA GAACCAATCA
    ATTTTCCATC AACAAAATAA TATGGCCCCA ATGAATCAAG AGCAGCAGCC CATGCAATTT
    CAGAACCAGA CCACGGTTTC CTCACTTCAG AACCCAGGTC CTGCCCAATC CGAATCATCA
    CAGACCTCCT TGTTCCATAG CTCACCTCAG ATTCAGTTGG TCCAAGGGTC ACCCAGTTCT
    CAAGAGCAGC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAGACCAGT
    ATGAACCAAC AAGACATGCA ACAGTCTCCC CTTTATTCCC CTCAGAACAA CATGCCTGGA
    ATCCAGGGAG CCACATCTTC ACCTCAACCA CAGGCTACTT TATTTCACAA CACTACAGGA
    GGTACAATGA ACCAGCTACA GAATTCTCCT GGCTCTTCTC AACAGACATC TGGAATGTTC
    TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACTT CTGGACCAGC TACATTGCCA
    GATCAGTTGA TGGCCATAAG TCCACCAGGC CAGCCACAAA ACGAGGGCCA ACCACCTGTG
    ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAA TGGCATCCTC TATAAACACC
    AACCAGAACA TTGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTA
    ACTGGCTCCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53918
    Clone ID Related Accession (Same CDS sequence) XM_010814692.2 , XM_015467393.1
    Accession Version XM_015467393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nuclear factor of activated T-cells 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTC CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CTGTTGCTTT GAATGTGAAT GTAAAGAAGG AAATATCTAG TCCGGCAAGA
    CCTTGCTCTT TCGAAGAAGC CATGAAAGCA ATGAAAACTA CTGGATGTAA CTTAGATAAG
    GTAAATATGC TTCCTAATGC TCTGATCACT CCGCTCATAT CAAGCACTAT GATTAAGAGT
    GAAGACATTA CTCCAATGGA AGTAACAGCA GAGAAAAGAT CGCCTTCTAT TTTTAAGACT
    ACAAAGACAG TTGGATCAAC TCAACAAACG TTAGAAAATC TCTCTCACAT AGCAGGAAAT
    GGGTCTTTTT CATCATCATC TTCCCACTTA ACTTCTGAAA ATGAAAAGCA ACAACAGATT
    CAGCCCAAGG CATACAACCC AGAGACCCTG ACAACTATCC AAACACAGGA CATTTCACAG
    CCTGGTACTT TTCCAGCGGT TTCTGCGTCT AGTCAGCTGC CCAGCAATGA CGCACTACTA
    CAGCAGGCTA CACAGTTTCA AACAAGAGAA ACTCAGTCTA GAGAGGTGTT ACAGTCAGAT
    GGTACAGTGG TTAACTTGTC ACACCTGACT GAGACATCAC AGCAACAGCA GCAGTCTCCA
    CTGCAAGAAC AAGCACAGAC TTTACAGCAG CAGATTTCAT CAAATATTTT CCCATCACCA
    AATAGTGTGA GTCAGCAACT ACAAAATACG ATACAACACT TGCAGGCAGG GAGTTTTACA
    GGCAGTACTG CCAGTGGCAG CAATGGAAAT GTTGACTTGG TCCAACAAGT TTTAGAGGCA
    CAACAGCAGT TATCTTCAGT TTTATTTTCT GCTCCAGATG GTAACGAGAA TGTTCAAGAA
    CAGCTTAGTG CAGACATTTT TCAACAAGTC AGTCAAATTC AAAATAGCGT AAGCCCTGGA
    ATGTTTTCCT CAACAGAACC AGCAGTCCAT ACCAGACCAG ATAATTTAAT AGCTGGAAGA
    GCTGAAAGTG TTCACCCACA GAATGAAAAC ACATTGTCTA ATCAGCAACA GCAACAGCAG
    CAACAGCAAG TGATGGACTC CTCAGCTGCA ATGGTGATGG AGATGCAACA GAGTATCTGC
    CAGGCAGCTG CCCAGATTCA GTCAGAGCTG TTTCCTTCAT CTGCTTCAGC AAATGGAAAC
    CTTCAGCAGT CTCCAGTTTA CCAGCAGACT TCTCATATGA TGAGCGCATT ATCAGCCAAT
    GAGGACATGC AAATGCAATG TGAATTGTTT TCTTCTCCTC CGGCAGTTTC TGGAAATGAA
    ACTACTACAA CTACCACACA GCAGGTTGCA ACTTCTGGCA CTACCCTGTT TCAGACATCA
    AATTCAGGAG ATGGAGAAGA GACTGGAGCA CAAGCAAAAC AGATTCAGAA CAGTGTCTTT
    CAGACCATGG TCCAAATGCA ACATAGTGGG GACAGTCAGC CTCAAGTTGG CCTTTTTTCA
    TCTACAAAAA GTATGATAAG TGTTCAAAAT AGTGGTACCC AGCAGCAAGG CAATGGTTTA
    TTCCAGCAAG GTAATGAGAT GATGTCACTT CAATCAGGGA ATTTTTTGCA GCAGTCTTCT
    CATTCACAGG CTCAGCTTTT TCATCCTCAG AATCCTATTG CTGATCCTCA GAACCTTTCC
    CAGGAAACTC AAGGTTCTAT TTTTCATAGT CCAAGTCCTA TTGTCCACAG TCAGACTTCT
    ACAGCCTCCT CTGAACAAAT GCAGCCTCCA ATGTTTCACT CTCAAAATAC CATGGCTGTG
    CTGCAGGGCT CTTCTGTTCC TCAAGACCAA CAGTCAGCCA ACATATTTCT TTCCCAAAGT
    CCAATGAATA ATCTCCAAAC CAACACAGTG GCCCAGGAAG AACAGATTTC ATTTTTTGCA
    GCTCAGAATT CAATTTCTCC ACTTCAGTCA ACATCAAACA CTGAGCAGCA AGCTGCTTTC
    CAGCAGCAGG CTCCAATATC ACACATTCAG ACCCCTATGC TTTCCCAGGA ACAGGCACAA
    CCCTCCCAGC AAGGTCTGTT TCAGCCTCAG GTTTCCCTGG GCTCCCTTCC TCCTAACCCA
    ATGCCTCAAA ACCAACAAGG AACAATCTTT CAGTCACAGC ACTCAATAGT TGCCATCCAG
    AGTAACTCTC CATCCCAGGA GCAGCAGCAG CAGCAACAAC AGCAGCAGCA ACAACAGAGC
    ATTTTATTCA GTAACCAGAA CGCCATGGCA CCAATGGCGT CTCAAAAGCA GCCACCACCA
    AACATGATAT TCAATCCAAG TCAAAATCCA GTGGCTAATC AGGAGCAGCA GAACCAATCA
    ATTTTCCATC AACAAAATAA TATGGCCCCA ATGAATCAAG AGCAGCAGCC CATGCAATTT
    CAGAACCAGA CCACGGTTTC CTCACTTCAG AACCCAGGTC CTGCCCAATC CGAATCATCA
    CAGACCTCCT TGTTCCATAG CTCACCTCAG ATTCAGTTGG TCCAAGGGTC ACCCAGTTCT
    CAAGAGCAGC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAGACCAGT
    ATGAACCAAC AAGACATGCA ACAGTCTCCC CTTTATTCCC CTCAGAACAA CATGCCTGGA
    ATCCAGGGAG CCACATCTTC ACCTCAACCA CAGGCTACTT TATTTCACAA CACTACAGGA
    GGTACAATGA ACCAGCTACA GAATTCTCCT GGCTCTTCTC AACAGACATC TGGAATGTTC
    TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACTT CTGGACCAGC TACATTGCCA
    GATCAGTTGA TGGCCATAAG TCCACCAGGC CAGCCACAAA ACGAGGGCCA ACCACCTGTG
    ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAA TGGCATCCTC TATAAACACC
    AACCAGAACA TTGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTA
    ACTGGCTCCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322879.1
    CDS98..4732
    Translation

    Target ORF information:

    RefSeq Version XM_015467393.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCCTCGG ACTTCATCTC ATTGCTCAGC GCGGACCTAG ACCTGGAGTC GCCCAAGTCC 
    CTGTACTCAC GAGATTCTCT GAAGTTACAC CCATCACAGA ATTTTCATAG AGCTGGACTA
    TTGGAAGAAT CTGTCTATGA TCTTCTCCCA AAGGAGTTAC AGTTACCTCC ATCTAGAGAA
    ACACCTGTAG CATCAATGAG TCAGACAAGT GGTGGTGAGG CAGGCTCGCC TCCTCCAGCT
    GTAGTTGCTG CTGATGCTTC TTCAGCTCCC TCCTCTTCCT CCATGGGCGG TGCTTGCAGC
    TCCTTTACCA CCTCTTCCAG CCCTACCATT TATTCTACCT CAGTCACCGA CAGCAAGGCT
    ATGCAAGTCG AGAGCTGCTC CTCAGCCTTG GGGGTAAGTA ACCGAGGGGT AAGTGAAAAG
    CAGTTAACCA GTAACACAGT TCAGCAGCAT CCATCAACAC CGAAGAGGCA CACAGTCTTG
    TACATCTCAC CACCACCTGA GGACTTGCTG GATAACAGTC GGATGTCCTG CCAGGATGAG
    GGGTGTGGAT TGGAATCTGA GCAGAGCTGC AGTATGTGGA TGGAGGATTC CCCCTCCAAC
    TTCAGTAACA TGAGCACCAG TTCCTACAAT GATAACACTG AGGTACCTCG TAAATCACGA
    AAACGAAATC CAAAGCAGAG GCCGGGGGTC AAACGACGAG ATTGTGAAGA ATCTAATATG
    GATATATTTG ATGCCGACAG TGCCAAAGCA CCTCACTATG TGCTTTCTCA GCTTACCACG
    GACAACAAAG GCAGCTCAAA AGCAGGAAAT GGAACATTGG AAAACCAAAA AGGAACTGGA
    GTAAAGAAGA GCCCTATGTT ATGTGGACAG TATCCTGTTA AAAGTGAGGG AAAGGAGCTG
    AAGATAGTTG TACAACCTGA GACCCAGCAC AGAGCTCGGT ACCTGACTGA GGGTAGTCGT
    GGCTCAGTAA AGGACAGAAC ACAGCAAGGC TTTCCTACAG TGAAGCTGGA AGGCCATAAT
    GAGCCAGTGG TGTTGCAGGT GTTTGTGGGC AATGACTCTG GTCGAGTGAA ACCACATGGA
    TTTTATCAGG CCTGCAGGGT AACTGGGCGA AATACAACTC CCTGCAAAGA AGTGGATATC
    GAAGGCACTA CTGTTATAGA AGTTGGCCTT GATCCAAGCA ACAATATGAC ACTGGCGGTT
    GACTGCGTAG GGATATTGAA ATTGAGGAAT GCTGATGTTG AAGCCAGAAT AGGAATTGCT
    GGTTCCAAGA AAAAGAGCAC TCGTGCCAGA TTGGTTTTTC GAGTTAATAT CACAAGGAAA
    GATGGCTCCA CTTTGACACT GCAAACACCC TCTTCTCCAA TTTTATGTAC TCAACCAGCA
    GGAGTGCCAG AAATCTTAAA GAAGAGCTTG CATAGCTGTT CGGTGAAAGG AGAGGAAGAA
    GTATTTTTAA TTGGCAAGAA CTTTCTGAAA GGAACTAAAG TTATTTTCCA AGAAAATGTT
    TCTGATGAAA ACTCTTGGAA GTCAGAAGCT GAAATTGATA TGGAATTATT TCATCAGAAT
    CATCTTATTG TGAAGGTTCC CCCATATCAT GATCAACATA TAACTTTGCC AGTAGCAGTG
    GGAATATATG TAGTAACCAA TGCTGGAAGA TCTCATGATG TTCAGCCTTT CACTTACACT
    CCAGACCCAG CTGTTGCTTT GAATGTGAAT GTAAAGAAGG AAATATCTAG TCCGGCAAGA
    CCTTGCTCTT TCGAAGAAGC CATGAAAGCA ATGAAAACTA CTGGATGTAA CTTAGATAAG
    GTAAATATGC TTCCTAATGC TCTGATCACT CCGCTCATAT CAAGCACTAT GATTAAGAGT
    GAAGACATTA CTCCAATGGA AGTAACAGCA GAGAAAAGAT CGCCTTCTAT TTTTAAGACT
    ACAAAGACAG TTGGATCAAC TCAACAAACG TTAGAAAATC TCTCTCACAT AGCAGGAAAT
    GGGTCTTTTT CATCATCATC TTCCCACTTA ACTTCTGAAA ATGAAAAGCA ACAACAGATT
    CAGCCCAAGG CATACAACCC AGAGACCCTG ACAACTATCC AAACACAGGA CATTTCACAG
    CCTGGTACTT TTCCAGCGGT TTCTGCGTCT AGTCAGCTGC CCAGCAATGA CGCACTACTA
    CAGCAGGCTA CACAGTTTCA AACAAGAGAA ACTCAGTCTA GAGAGGTGTT ACAGTCAGAT
    GGTACAGTGG TTAACTTGTC ACACCTGACT GAGACATCAC AGCAACAGCA GCAGTCTCCA
    CTGCAAGAAC AAGCACAGAC TTTACAGCAG CAGATTTCAT CAAATATTTT CCCATCACCA
    AATAGTGTGA GTCAGCAACT ACAAAATACG ATACAACACT TGCAGGCAGG GAGTTTTACA
    GGCAGTACTG CCAGTGGCAG CAATGGAAAT GTTGACTTGG TCCAACAAGT TTTAGAGGCA
    CAACAGCAGT TATCTTCAGT TTTATTTTCT GCTCCAGATG GTAACGAGAA TGTTCAAGAA
    CAGCTTAGTG CAGACATTTT TCAACAAGTC AGTCAAATTC AAAATAGCGT AAGCCCTGGA
    ATGTTTTCCT CAACAGAACC AGCAGTCCAT ACCAGACCAG ATAATTTAAT AGCTGGAAGA
    GCTGAAAGTG TTCACCCACA GAATGAAAAC ACATTGTCTA ATCAGCAACA GCAACAGCAG
    CAACAGCAAG TGATGGACTC CTCAGCTGCA ATGGTGATGG AGATGCAACA GAGTATCTGC
    CAGGCAGCTG CCCAGATTCA GTCAGAGCTG TTTCCTTCAT CTGCTTCAGC AAATGGAAAC
    CTTCAGCAGT CTCCAGTTTA CCAGCAGACT TCTCATATGA TGAGCGCATT ATCAGCCAAT
    GAGGACATGC AAATGCAATG TGAATTGTTT TCTTCTCCTC CGGCAGTTTC TGGAAATGAA
    ACTACTACAA CTACCACACA GCAGGTTGCA ACTTCTGGCA CTACCCTGTT TCAGACATCA
    AATTCAGGAG ATGGAGAAGA GACTGGAGCA CAAGCAAAAC AGATTCAGAA CAGTGTCTTT
    CAGACCATGG TCCAAATGCA ACATAGTGGG GACAGTCAGC CTCAAGTTGG CCTTTTTTCA
    TCTACAAAAA GTATGATAAG TGTTCAAAAT AGTGGTACCC AGCAGCAAGG CAATGGTTTA
    TTCCAGCAAG GTAATGAGAT GATGTCACTT CAATCAGGGA ATTTTTTGCA GCAGTCTTCT
    CATTCACAGG CTCAGCTTTT TCATCCTCAG AATCCTATTG CTGATCCTCA GAACCTTTCC
    CAGGAAACTC AAGGTTCTAT TTTTCATAGT CCAAGTCCTA TTGTCCACAG TCAGACTTCT
    ACAGCCTCCT CTGAACAAAT GCAGCCTCCA ATGTTTCACT CTCAAAATAC CATGGCTGTG
    CTGCAGGGCT CTTCTGTTCC TCAAGACCAA CAGTCAGCCA ACATATTTCT TTCCCAAAGT
    CCAATGAATA ATCTCCAAAC CAACACAGTG GCCCAGGAAG AACAGATTTC ATTTTTTGCA
    GCTCAGAATT CAATTTCTCC ACTTCAGTCA ACATCAAACA CTGAGCAGCA AGCTGCTTTC
    CAGCAGCAGG CTCCAATATC ACACATTCAG ACCCCTATGC TTTCCCAGGA ACAGGCACAA
    CCCTCCCAGC AAGGTCTGTT TCAGCCTCAG GTTTCCCTGG GCTCCCTTCC TCCTAACCCA
    ATGCCTCAAA ACCAACAAGG AACAATCTTT CAGTCACAGC ACTCAATAGT TGCCATCCAG
    AGTAACTCTC CATCCCAGGA GCAGCAGCAG CAGCAACAAC AGCAGCAGCA ACAACAGAGC
    ATTTTATTCA GTAACCAGAA CGCCATGGCA CCAATGGCGT CTCAAAAGCA GCCACCACCA
    AACATGATAT TCAATCCAAG TCAAAATCCA GTGGCTAATC AGGAGCAGCA GAACCAATCA
    ATTTTCCATC AACAAAATAA TATGGCCCCA ATGAATCAAG AGCAGCAGCC CATGCAATTT
    CAGAACCAGA CCACGGTTTC CTCACTTCAG AACCCAGGTC CTGCCCAATC CGAATCATCA
    CAGACCTCCT TGTTCCATAG CTCACCTCAG ATTCAGTTGG TCCAAGGGTC ACCCAGTTCT
    CAAGAGCAGC AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAGACCAGT
    ATGAACCAAC AAGACATGCA ACAGTCTCCC CTTTATTCCC CTCAGAACAA CATGCCTGGA
    ATCCAGGGAG CCACATCTTC ACCTCAACCA CAGGCTACTT TATTTCACAA CACTACAGGA
    GGTACAATGA ACCAGCTACA GAATTCTCCT GGCTCTTCTC AACAGACATC TGGAATGTTC
    TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACTT CTGGACCAGC TACATTGCCA
    GATCAGTTGA TGGCCATAAG TCCACCAGGC CAGCCACAAA ACGAGGGCCA ACCACCTGTG
    ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAA TGGCATCCTC TATAAACACC
    AACCAGAACA TTGAAAAGAT TGATTTGCTT GTTTCATTGC AAAACCAAGG GAACAACTTA
    ACTGGCTCCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.