TCAF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TCAF1
Entrez Gene ID	533126
Full Name	TRPM8 channel associated factor 1
Synonyms	FAM115A
General protein information	Preferred Names TRPM8 channel associated factor 1 Names TRPM8 channel-associated factor 1 Protein FAM115A TRP channel-associated factor 1 family with sequence similarity 115, member A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005205866.3	XP_005205923.1	TRPM8 channel-associated factor 1 isoform X1
NM_001099054.1	NP_001092524.1	TRPM8 channel-associated factor 1
XM_005205866.4	XP_005205923.1	TRPM8 channel-associated factor 1 isoform X1
XM_005205865.3	XP_005205922.1	TRPM8 channel-associated factor 1 isoform X1
XM_005205867.3	XP_005205924.1	TRPM8 channel-associated factor 1 isoform X2
XM_005205867.4	XP_005205924.1	TRPM8 channel-associated factor 1 isoform X2
XM_005205865.4	XP_005205922.1	TRPM8 channel-associated factor 1 isoform X1
XM_024990745.1	XP_024846513.1	TRPM8 channel-associated factor 1 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Fam115a	XP_003749787.1
Homo sapiens (human)	FAM115A	NP_055534.1
Pan troglodytes (chimpanzee)	FAM115A	XP_003318910.1
Canis lupus familiaris (dog)	LOC482730	XP_005629583.1
Mus musculus (house mouse)	Fam115a	NP_084206.1
Bos taurus (cattle)	FAM115A	NP_001092524.1

Gene Ontology
Bibliography

Related articles in PubMed

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.
Gkika D, Lemonnier L, Shapovalov G, Gordienko D, Poux C, Bernardini M, Bokhobza A, Bidaux G, Degerny C, Verreman K, Guarmit B, Benahmed M, de Launoit Y, Bindels RJ, Fiorio Pla A, Prevarskaya N
The Journal of cell biology208(1)89-107(2015 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TCAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43810	NM_001099054.1 Latest version!	Bos taurus TRPM8 channel associated factor 1 (TCAF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa18880	XM_005205866.4 Latest version!	Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa18880	XM_005205865.4 Latest version!	Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa18881	XM_024990745.1 Latest version!	Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa18881	XM_005205867.4 Latest version!	Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OBa18880	XM_005205866.3	Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa18881	XM_005205867.3	Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa18880	XM_005205865.3	Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TCAF1 ( NM_001099054.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001092524.1 Bos taurus TRPM8 channel associated factor 1 (TCAF1), mRNA.

Price & Availability↑

CloneID	OBa43810
Clone ID Related Accession (Same CDS sequence)	NM_001099054.1
Accession Version	NM_001099054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-11
Organism	Bos taurus(cattle)
Product	TRPM8 channel-associated factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC142182.1. On Jun 26, 2007 this sequence version replaced XM_863840.2. ##Evidence-Data-START## Transcript exon combination :: BC142182.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGGA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092524.1
CDS	114..2879
Translation	MATPSAAFEALMNGVTSWNVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPVGVHPSLAPLAKILEGSGVEAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKRGGGLLIGGQAWDWANQGDDERVLFTFPGNLVTSVAGVYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSEDRDELLHGISELDITNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDAGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSDVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCNNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQSSKLGTVPVTIKGAVRAPYYKLGETTQEEWKRQIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRGRANIALWPPVREKRVRIYLGKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTDNVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWEENIMKLYLLTQMPH

Target ORF information:

RefSeq Version	NM_001099054.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRPM8 channel associated factor 1 (TCAF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099054.1

ORF Insert Sequence:

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGGA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCAF1 ( XM_005205866.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205923.1 Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa18880
Clone ID Related Accession (Same CDS sequence)	XM_005205866.3 , XM_005205866.4 , XM_005205865.3 , XM_005205865.4
Accession Version	XM_005205866.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRPM8 channel-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205923.1
CDS	280..3045
Translation	MATPSAAFEALMNGVTSWNVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPVGVHPSLAPLAKILEGSGVEAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKRGGGLLIGGQAWDWANQGDDERVLFTFPGNLVTSVAGVYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSEDRDELLHGISELDITNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDAGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSDVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCNNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQSSKLGTVPVTIKGAVRAPYYKLGETTQEEWKRQIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRGRANIALWPPVREKRVRIYLGKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTDNVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQMPH

Target ORF information:

RefSeq Version	XM_005205866.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205866.3

ORF Insert Sequence:

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCAF1 ( XM_005205866.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205923.1 Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa18880
Clone ID Related Accession (Same CDS sequence)	XM_005205866.3 , XM_005205866.4 , XM_005205865.3 , XM_005205865.4
Accession Version	XM_005205866.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRPM8 channel-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205866.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205923.1
CDS	237..3002
Translation	MATPSAAFEALMNGVTSWNVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPVGVHPSLAPLAKILEGSGVEAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKRGGGLLIGGQAWDWANQGDDERVLFTFPGNLVTSVAGVYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSEDRDELLHGISELDITNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDAGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSDVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCNNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQSSKLGTVPVTIKGAVRAPYYKLGETTQEEWKRQIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRGRANIALWPPVREKRVRIYLGKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTDNVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQMPH

Target ORF information:

RefSeq Version	XM_005205866.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205866.4

ORF Insert Sequence:

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCAF1 ( XM_005205865.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205922.1 Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18880
Clone ID Related Accession (Same CDS sequence)	XM_005205866.3 , XM_005205866.4 , XM_005205865.3 , XM_005205865.4
Accession Version	XM_005205865.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRPM8 channel-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205922.1
CDS	157..2922
Translation	MATPSAAFEALMNGVTSWNVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPVGVHPSLAPLAKILEGSGVEAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKRGGGLLIGGQAWDWANQGDDERVLFTFPGNLVTSVAGVYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSEDRDELLHGISELDITNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDAGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSDVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCNNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQSSKLGTVPVTIKGAVRAPYYKLGETTQEEWKRQIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRGRANIALWPPVREKRVRIYLGKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTDNVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQMPH

Target ORF information:

RefSeq Version	XM_005205865.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205865.3

ORF Insert Sequence:

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCAF1 ( XM_005205865.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205922.1 Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18880
Clone ID Related Accession (Same CDS sequence)	XM_005205866.3 , XM_005205866.4 , XM_005205865.3 , XM_005205865.4
Accession Version	XM_005205865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRPM8 channel-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205922.1
CDS	168..2933
Translation	MATPSAAFEALMNGVTSWNVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPVGVHPSLAPLAKILEGSGVEAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKRGGGLLIGGQAWDWANQGDDERVLFTFPGNLVTSVAGVYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSEDRDELLHGISELDITNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDAGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSDVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCNNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQSSKLGTVPVTIKGAVRAPYYKLGETTQEEWKRQIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRGRANIALWPPVREKRVRIYLGKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTDNVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQMPH

Target ORF information:

RefSeq Version	XM_005205865.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205865.4

ORF Insert Sequence:

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCAF1 ( XM_005205867.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205924.1 Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa18881
Clone ID Related Accession (Same CDS sequence)	XM_005205867.3 , XM_005205867.4 , XM_024990745.1
Accession Version	XM_005205867.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	TRPM8 channel-associated factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205924.1
CDS	137..2896
Translation	MATPSAAFEALMNGVTSWNVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPVGVHPSLAPLAKILEGSGVEAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKRGGGLLIGGQAWDWANQGDDERVLFTFPGNLVTSVAGVYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSEDRDELLHGISELDITNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDAGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSDVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCNNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQSSKLGTVPVTIKGAVRAPYYKLGETTQEEWKRQIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRGRANIALWPPVREKRVRIYLGKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTDNVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQM

Target ORF information:

RefSeq Version	XM_005205867.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205867.3

ORF Insert Sequence:

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCAF1 ( XM_005205867.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205924.1 Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa18881
Clone ID Related Accession (Same CDS sequence)	XM_005205867.3 , XM_005205867.4 , XM_024990745.1
Accession Version	XM_005205867.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRPM8 channel-associated factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205924.1
CDS	142..2901
Translation	MATPSAAFEALMNGVTSWNVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPVGVHPSLAPLAKILEGSGVEAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKRGGGLLIGGQAWDWANQGDDERVLFTFPGNLVTSVAGVYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSEDRDELLHGISELDITNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDAGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSDVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCNNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQSSKLGTVPVTIKGAVRAPYYKLGETTQEEWKRQIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRGRANIALWPPVREKRVRIYLGKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTDNVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQM

Target ORF information:

RefSeq Version	XM_005205867.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205867.4

ORF Insert Sequence:

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCAF1 ( XM_024990745.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846513.1 Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa18881
Clone ID Related Accession (Same CDS sequence)	XM_005205867.3 , XM_005205867.4 , XM_024990745.1
Accession Version	XM_024990745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TRPM8 channel-associated factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846513.1
CDS	169..2928
Translation	MATPSAAFEALMNGVTSWNVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPVGVHPSLAPLAKILEGSGVEAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKRGGGLLIGGQAWDWANQGDDERVLFTFPGNLVTSVAGVYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSEDRDELLHGISELDITNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDAGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSDVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCNNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQSSKLGTVPVTIKGAVRAPYYKLGETTQEEWKRQIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRGRANIALWPPVREKRVRIYLGKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTDNVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQM

Target ORF information:

RefSeq Version	XM_024990745.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990745.1

ORF Insert Sequence:

ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG 
GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG 
GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA 
GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT 
CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC 
TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG 
TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG 
GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT 
GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC 
AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG 
CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG 
CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT 
GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT 
AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG 
ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC 
ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT 
GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA 
GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA 
GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG 
CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG 
TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG 
GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG 
GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG 
AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT 
CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC 
TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC 
AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC 
CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC 
CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC 
AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG 
AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC 
AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC 
TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC 
CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA 
TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC 
TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG 
CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC 
CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC 
CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT 
GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT 
GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT 
GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA 
GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT 
CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG 
GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.
The Journal of cell biology208(1)89-107(2015 Jan)
Gkika D,Lemonnier L,Shapovalov G,Gordienko D,Poux C,Bernardini M,Bokhobza A,Bidaux G,Degerny C,Verreman K,Guarmit B,Benahmed M,de Launoit Y,Bindels RJ,Fiorio Pla A,Prevarskaya N

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TCAF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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