Tcaf1 cDNA ORF clone, Mus musculus(house mouse)

The following Tcaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu07696 NM_001355769.1
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu07696 NM_029930.3
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu07696 NM_001355770.1
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu37664 NM_001355768.1
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu07696 XM_006506755.2
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu07696 XM_017321830.1
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu37664 XM_006506751.3
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu07696 XM_006506756.3
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu37664 XM_006506753.4
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu37664 XM_006506752.4
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu37664 XM_006506754.4
Latest version!
Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OMu37664 XM_006506752.3 Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu07696 NM_029930.2 Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu37664 XM_006506753.3 Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu37664 XM_006506754.3 Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu07696 XM_006506756.2 Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu07696
    Clone ID Related Accession (Same CDS sequence) XM_006506755.2 , XM_017321830.1 , NM_001355769.1 , XM_006506756.2 , NM_029930.2 , NM_029930.3 , NM_001355770.1 , XM_006506756.3
    Accession Version NM_001355769.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC155334.7. On Sep 27, 2017 this sequence version replaced XM_006506755.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK173013.1, SRR1660811.117950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342698.1
    CDS172..2940
    Translation

    Target ORF information:

    RefSeq Version NM_001355769.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07696
    Clone ID Related Accession (Same CDS sequence) XM_006506755.2 , XM_017321830.1 , NM_001355769.1 , XM_006506756.2 , NM_029930.2 , NM_029930.3 , NM_001355770.1 , XM_006506756.3
    Accession Version NM_029930.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-28
    Organism Mus musculus(House mouse)
    Product TRPM8 channel-associated factor 1
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK053509.1. On Apr 5, 2007 this sequence version replaced NM_029930.1. ##Evidence-Data-START## Transcript exon combination :: AK053509.1, AK148593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_084206.1
    CDS142..2910
    Translation

    Target ORF information:

    RefSeq Version NM_029930.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_029930.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07696
    Clone ID Related Accession (Same CDS sequence) XM_006506755.2 , XM_017321830.1 , NM_001355769.1 , XM_006506756.2 , NM_029930.2 , NM_029930.3 , NM_001355770.1 , XM_006506756.3
    Accession Version NM_029930.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-07
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY324097.1 and AK053509.1. On Sep 27, 2017 this sequence version replaced NM_029930.2. ##Evidence-Data-START## Transcript exon combination :: AK053509.1, AK148593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_084206.1
    CDS183..2951
    Translation

    Target ORF information:

    RefSeq Version NM_029930.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_029930.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07696
    Clone ID Related Accession (Same CDS sequence) XM_006506755.2 , XM_017321830.1 , NM_001355769.1 , XM_006506756.2 , NM_029930.2 , NM_029930.3 , NM_001355770.1 , XM_006506756.3
    Accession Version NM_001355770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-22
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC155334.7. On Sep 27, 2017 this sequence version replaced XM_017321830.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR1660811.117950.1, SRR1660811.24866.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342699.1
    CDS281..3049
    Translation

    Target ORF information:

    RefSeq Version NM_001355770.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37664
    Clone ID Related Accession (Same CDS sequence) XM_006506753.3 , XM_006506752.3 , XM_006506752.4 , XM_006506751.3 , NM_001355768.1 , XM_006506754.3 , XM_006506753.4 , XM_006506754.4
    Accession Version NM_001355768.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-07
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK014379.1, AK132148.1 and BM117796.2. On Sep 27, 2017 this sequence version replaced XM_006506751.3. ##Evidence-Data-START## Transcript exon combination :: AK132148.1, AK083917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342697.1
    CDS172..2946
    Translation

    Target ORF information:

    RefSeq Version NM_001355768.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07696
    Clone ID Related Accession (Same CDS sequence) XM_006506755.2 , XM_017321830.1 , NM_001355769.1 , XM_006506756.2 , NM_029930.2 , NM_029930.3 , NM_001355770.1 , XM_006506756.3
    Accession Version XM_006506755.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506818.1
    CDS169..2937
    Translation

    Target ORF information:

    RefSeq Version XM_006506755.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506755.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07696
    Clone ID Related Accession (Same CDS sequence) XM_006506755.2 , XM_017321830.1 , NM_001355769.1 , XM_006506756.2 , NM_029930.2 , NM_029930.3 , NM_001355770.1 , XM_006506756.3
    Accession Version XM_017321830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177319.1
    CDS278..3046
    Translation

    Target ORF information:

    RefSeq Version XM_017321830.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07696
    Clone ID Related Accession (Same CDS sequence) XM_006506755.2 , XM_017321830.1 , NM_001355769.1 , XM_006506756.2 , NM_029930.2 , NM_029930.3 , NM_001355770.1 , XM_006506756.3
    Accession Version XM_006506756.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006506756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506819.1
    CDS200..2968
    Translation

    Target ORF information:

    RefSeq Version XM_006506756.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506756.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37664
    Clone ID Related Accession (Same CDS sequence) XM_006506753.3 , XM_006506752.3 , XM_006506752.4 , XM_006506751.3 , NM_001355768.1 , XM_006506754.3 , XM_006506753.4 , XM_006506754.4
    Accession Version XM_006506753.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506816.1
    CDS201..2975
    Translation

    Target ORF information:

    RefSeq Version XM_006506753.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506753.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37664
    Clone ID Related Accession (Same CDS sequence) XM_006506753.3 , XM_006506752.3 , XM_006506752.4 , XM_006506751.3 , NM_001355768.1 , XM_006506754.3 , XM_006506753.4 , XM_006506754.4
    Accession Version XM_006506752.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506815.1
    CDS279..3053
    Translation

    Target ORF information:

    RefSeq Version XM_006506752.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506752.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37664
    Clone ID Related Accession (Same CDS sequence) XM_006506753.3 , XM_006506752.3 , XM_006506752.4 , XM_006506751.3 , NM_001355768.1 , XM_006506754.3 , XM_006506753.4 , XM_006506754.4
    Accession Version XM_006506751.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506751.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506814.1
    CDS170..2944
    Translation

    Target ORF information:

    RefSeq Version XM_006506751.3
    Organism Mus musculus(House mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506751.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37664
    Clone ID Related Accession (Same CDS sequence) XM_006506753.3 , XM_006506752.3 , XM_006506752.4 , XM_006506751.3 , NM_001355768.1 , XM_006506754.3 , XM_006506753.4 , XM_006506754.4
    Accession Version XM_006506754.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506817.1
    CDS282..3056
    Translation

    Target ORF information:

    RefSeq Version XM_006506754.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506754.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07696
    Clone ID Related Accession (Same CDS sequence) XM_006506755.2 , XM_017321830.1 , NM_001355769.1 , XM_006506756.2 , NM_029930.2 , NM_029930.3 , NM_001355770.1 , XM_006506756.3
    Accession Version XM_006506756.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506756.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506819.1
    CDS220..2988
    Translation

    Target ORF information:

    RefSeq Version XM_006506756.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506756.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37664
    Clone ID Related Accession (Same CDS sequence) XM_006506753.3 , XM_006506752.3 , XM_006506752.4 , XM_006506751.3 , NM_001355768.1 , XM_006506754.3 , XM_006506753.4 , XM_006506754.4
    Accession Version XM_006506752.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506752.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506815.1
    CDS297..3071
    Translation

    Target ORF information:

    RefSeq Version XM_006506752.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506752.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37664
    Clone ID Related Accession (Same CDS sequence) XM_006506753.3 , XM_006506752.3 , XM_006506752.4 , XM_006506751.3 , NM_001355768.1 , XM_006506754.3 , XM_006506753.4 , XM_006506754.4
    Accession Version XM_006506753.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506753.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506816.1
    CDS220..2994
    Translation

    Target ORF information:

    RefSeq Version XM_006506753.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506753.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37664
    Clone ID Related Accession (Same CDS sequence) XM_006506753.3 , XM_006506752.3 , XM_006506752.4 , XM_006506751.3 , NM_001355768.1 , XM_006506754.3 , XM_006506753.4 , XM_006506754.4
    Accession Version XM_006506754.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506754.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506817.1
    CDS300..3074
    Translation

    Target ORF information:

    RefSeq Version XM_006506754.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506754.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACTC CCTCTGCTGC CTTTGAGGCC CTTATGAATG GAGTAACGAG TTGGGATATC 
    CCCGAGGATT CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTATAATG
    GTGAATGACG TGGGCCAGGT CCTCGTTGCC GCCTCTTCCT ATGGCAGAGG CCGCATGGTG
    GTAGCATCTC ATGAGGACTT CTTGCTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT
    GTTGGGTGGC TTCGTTCCTC CCCTAACTCA GCCATTGGTG TGCACTCATC TTTAGCACCA
    TTGGTGAAAA TTCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAGCCAGA AGTCAATGAC
    TCCCTGGGGG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACAGATAA GCTAGTCCAG
    TTTGTGAAAC GTGGAGGGGG CTTGCTCATA GGAGGCGAAG CATGGGATTG GGATACCCAG
    GGTGATGATG ATAGAGTTCT GTTCGCATTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT
    GTGTACTTCA CTGACAACAA AGCAGACACA AGTTTCTTTA AAGTCTCCAA GAAGATGCCT
    AAGATACCCA TCTTAGTTCG TTGTGATGAC GACCTCTCAG ATGACAGAGA TGAGCTTCTG
    CGTGGCATAA TAGACCTGGA CATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTT
    CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCTACC ATGGCTGTGT GATAGCTGCA
    GCTCGCTATG GCCGGGGTCG GGTAGTTGTA ACTGGTCATA AGGTTTTATT TACTGTGGGT
    AAACTCGGCC CCTTTTTGCT CAATGCTGTC CGCTGGCTGG ATGGTGGCCG CAAAGGCAAG
    ATTGTGGTAC AGACAGAGCT GAGAACACTA AGTGGTCTGC TCGCAGTAGG GGGTATAGAC
    ACCAGCATTG AGCCCCACCT GACCAGTGAC GCAAGTGTCT ATTGTTTTGA ACCCACAAGC
    GATGTGGGGG TTAAGGAACT ACAGGAGTTT GTAGCAGAGG GTGGTGGGCT GTTTGTTGGA
    ACTCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCTCTGGC TCGATTCCCA
    GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAGAGTCT CAATCCAGGA
    CCTTTCCGTA CCCCTAAAAT AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTAGCAGAG
    TTCCAGGTGA TAATGGGCAG GAAGAGAGGG AATGTGGAGA AGGGCTGGCT AGCCAAGCTA
    GGCCCAGATG GGGCAACTTT CCTGCAGATT CCTGCAGAAG AGATCCCTGC TTACATGTCT
    GTGCACAGAC TCCTGAGGAA GCTGCTGAGC CGCTATCGGC TTCCAGTGGC AACCAGAGAG
    AACCCGGTCA TCAATGACTG TTGTAGGGGA GCCATGCTTT CCCTGGCCAC AGGTCTGGCC
    CACTCTGGAA GTGACCTCTC ACTGCTGATC CCAGAAATTG AAGACGTGTA CAGCAGCCGC
    TACCTGCGCC CCTCAGCGTC TCCCATCACC GTGAATGTCA ATTGCACTAA TCCAGGCACT
    CGATATTGCT GGATGAGCAC CGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTCTCC
    GTGCCTGAGA GTGCTGCCTC TGCTGACCTG AAGATACAAA TTGGCTGTCA TACTGATGAT
    CTGACAAGGG CCAGCAAGCT GTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAC
    AAACCCACGA AATCAATCAC CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG
    AATAGCAAAC TGGGTTCTGT GCCCCTTACA GTGAAGGGGG CTGTTCGTGC TCCATTCTAC
    AAGCTAGGGG AGACATCCAA GGAAGAATGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCA
    TGGGGAGAAC TGGCCACTGA CAACATCATC CTGACTGTGC CCACTGCAAA TCTTCGAACG
    CTGGAGAATC CTGAGCCACT GCTCCGCCTC TGGGATGAGA TGATGCAGGC TGTGGCTAAG
    CTGGGAGGCG AGACCTTCCC TTTGCGTCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC
    TCAGTAGGCT GGATGCACGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAA
    CTCATCAACG AGAAGCTCAT CCGGACCAAG GGGCTCTGGG GCCCTGTGCA TGAGCTGGGC
    CGCAATCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACGTGCAAC
    CTGTGGTGTG TTTACGTGCA TGAAACGGTC CTGGGCATAC CCCGAAGCCG TGCCAATATT
    GCGCTATGGC CTCCAGTTCG GGAGAAGAGA GTCCGAATCT ACTTGAGCAA GGGACCCAGT
    GTGAAACACT GGAACGCCTG GACTGCGTTG GAAACGTACC TACAGCTCCA AGAGGCCTTT
    GGTTGGGAGC CATTTATTCG TCTCTTCACC GAGTACAGAA ACCAGACAAC ATCGAGCTCG
    CCGCCCTCAG ACAACGTCGA CAAAATGAAT CTATGGGTCA AGATGTTCTC CCAGCAAGTG
    CAGAGGAATC TGGCTCCATT CTTTGAGGCC TGGGGCTGGC CTATCCAGAA GGAAGTGGCT
    ACAAGCTTAG CCTATCTGCC CGAATGGAAA GAGAATATAA TGAAATTGTA CCTCCTCACA
    CAGATGCCCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.