TCAF1 cDNA ORF clone, Bos taurus(cattle)

The following TCAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa43810 NM_001099054.1
Latest version!
Bos taurus TRPM8 channel associated factor 1 (TCAF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18880 XM_005205866.4
Latest version!
Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18880 XM_005205865.4
Latest version!
Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18881 XM_024990745.1
Latest version!
Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18881 XM_005205867.4
Latest version!
Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OBa18880 XM_005205866.3 Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18881 XM_005205867.3 Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18880 XM_005205865.3 Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa43810
    Clone ID Related Accession (Same CDS sequence) NM_001099054.1
    Accession Version NM_001099054.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-11
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC142182.1. On Jun 26, 2007 this sequence version replaced XM_863840.2. ##Evidence-Data-START## Transcript exon combination :: BC142182.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGGA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001092524.1
    CDS114..2879
    Translation

    Target ORF information:

    RefSeq Version NM_001099054.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 1 (TCAF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001099054.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGGA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18880
    Clone ID Related Accession (Same CDS sequence) XM_005205866.3 , XM_005205866.4 , XM_005205865.3 , XM_005205865.4
    Accession Version XM_005205866.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205923.1
    CDS280..3045
    Translation

    Target ORF information:

    RefSeq Version XM_005205866.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18880
    Clone ID Related Accession (Same CDS sequence) XM_005205866.3 , XM_005205866.4 , XM_005205865.3 , XM_005205865.4
    Accession Version XM_005205866.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205866.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205923.1
    CDS237..3002
    Translation

    Target ORF information:

    RefSeq Version XM_005205866.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205866.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18880
    Clone ID Related Accession (Same CDS sequence) XM_005205866.3 , XM_005205866.4 , XM_005205865.3 , XM_005205865.4
    Accession Version XM_005205865.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205922.1
    CDS157..2922
    Translation

    Target ORF information:

    RefSeq Version XM_005205865.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205865.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18880
    Clone ID Related Accession (Same CDS sequence) XM_005205866.3 , XM_005205866.4 , XM_005205865.3 , XM_005205865.4
    Accession Version XM_005205865.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205922.1
    CDS168..2933
    Translation

    Target ORF information:

    RefSeq Version XM_005205865.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205865.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18881
    Clone ID Related Accession (Same CDS sequence) XM_005205867.3 , XM_005205867.4 , XM_024990745.1
    Accession Version XM_005205867.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205924.1
    CDS137..2896
    Translation

    Target ORF information:

    RefSeq Version XM_005205867.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel-associated factor 1 (TCAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18881
    Clone ID Related Accession (Same CDS sequence) XM_005205867.3 , XM_005205867.4 , XM_024990745.1
    Accession Version XM_005205867.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205924.1
    CDS142..2901
    Translation

    Target ORF information:

    RefSeq Version XM_005205867.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205867.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18881
    Clone ID Related Accession (Same CDS sequence) XM_005205867.3 , XM_005205867.4 , XM_024990745.1
    Accession Version XM_024990745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846513.1
    CDS169..2928
    Translation

    Target ORF information:

    RefSeq Version XM_024990745.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TRPM8 channel associated factor 1 (TCAF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CATCTGCTGC CTTCGAGGCC CTTATGAATG GAGTGACAAG CTGGAACGTC 
    CCCGAAGACG CCGTCCCGTG TGAACTGCTT CTTATCGGAG AAGCCTCCTT CCCGGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATCGCG GCCTCCTCCT ATGGCCGAGG CCGCCTGGTG
    GTGGTGTCCC ACGAGGACTA CTTGGTGGAG GCCCAGCTCA CTCCCTTTCT CCTCAACGCA
    GTGGGTTGGC TTTGCTCTTC CCCCGGGGCT CCCGTGGGTG TGCACCCCTC CCTGGCACCT
    CTGGCCAAAA TCCTGGAGGG CTCTGGAGTG GAGGCAAAGG TTGAGCCCGA AGTGAAAGAC
    TCCCTGGGCG TTTACTGCAT TGATGCCTAC AATGAAACCA TGACTGAGAA GTTAGTCAAG
    TTCATGAAAC GTGGAGGAGG CTTGCTTATT GGAGGCCAAG CCTGGGACTG GGCCAACCAG
    GGCGATGATG AAAGGGTTCT GTTCACGTTC CCAGGGAACC TCGTGACCAG CGTGGCCGGT
    GTGTACTTCA CCGACAACAA AGGCGACACG AGCTTCTTTA AAGTGTCTAA GAAGATGCCC
    AAGATCCCAG TCTTGGTGAG TTGTGAGGAT GATCTCTCCG AGGACAGAGA TGAGCTCCTG
    CACGGGATTT CTGAGCTGGA CATCACCAAT TCGGACTGTT TCCCATCCCA GCTGCTAGTG
    CACGGGGCCT TAGCCTTTCC CCTGGGGCTA GATTCCTACC ACGGCTGTGT CATAGCGGCT
    GCCCGCTACG GCCGGGGCAG GGTGGTCGTG ACCGGCCACA AGGTCCTATT CACCGTGGGT
    AAACTGGGCC CCTTCTTGCT CAACGCCGTC CGCTGGCTGG ACGCGGGCCG CAGAGGCAAG
    ATCGTGGTGC AGACGGAGCT GAGGACTCTG AGTGGGCTGC TCGCCGTGGG GGGCATAGAC
    ACCAGCATTG AGCCCAATCT GACCAGCGAT GCCAGTGTCT ACTGCTTTGA GCCCGTGAGT
    GATGTAGGAG TCAAGGAGCT GCAGGAGTTT GTAGCCGAAG GCGGGGGCCT GTTTGTCGGA
    GCCCAGGCCT GGTGGTGGGC CTTCAAGAAC CCAGGCGTAT CCCCTTTGGC GCGGTTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATT AGCATCACCA GCCAAAGCCT CAACCCGGGG
    CCCTTTCGTA CTCCCAAGGC GGGGATAAGG ACCTACCATT TCCGCTCCAC CCTGGCTGAG
    TTCCAGGTTA TCATGGGCAG GAAGAGAGGG AACGTGGAGA AGGGCTGGTT GGCCAAGCTG
    GGTCCGGACG GCGCGGCTTT TCTGCAAATC CCCGCGGAGG AGATCCCCGC CTACATGTCG
    GTGCACCGGC TTCTAAGGAA GCTGCTGAGT CGCTATCGGC TCCCGGTGGC GACCCGTGAG
    AATCCCGTCA TCAACGACTG CTGCCGGGGT GCCATGCTCT CCCTGGCCAC TGGGCTGGCT
    CACTCGGGAA GCGACCTGTC TCTATTAGTC CCCGAAATTG AAGACATGTA CAGCAGCCCC
    TATCTGCGCC CCTCGGAATC TCCCATCACC GTGGAGGTCA ACTGCAACAA CCCTGGCACC
    AGGTACTGCT GGATGAGCAC CGGGCTCTAC ATACCCGGAA GGCAAATTAT TGAGGTCTCC
    CTGCCTGAAG CTGCCGCCTC TGCTGACCTG AAGATACAGA TTGGCTGCCA CACCGATGAC
    CTGACCAGGG CCAGCAAGCT GTTCCGAGGC CCCCTGGTGA TTAACCGCTG CTGCCTGGAC
    AAACCCACCA AGTCCATCAC CTGCCTCTGG GGGGGCCTCC TGTACATCAT CGTGCCTCAG
    AGCAGCAAGC TGGGCACCGT GCCTGTCACC ATTAAGGGAG CTGTGCGTGC TCCCTACTAC
    AAGCTAGGGG AGACAACCCA GGAGGAGTGG AAGCGGCAGA TCCAGGAGAA CCCAGGTCCC
    TGGGGAGAGC TGGCCACAGA CAACATCATC CTGACGGTGC CGACCGCGAA CCTCCGCACC
    CTGGAGAACC CCGAGCCGCT GCTTCGCCTC TGGGATGAGG TGATGCAGGC CGTGGCCCGA
    TTGGGGGCCG AGCCCTTCCC ACTGCGTCTG CCTCAGAGGA TCGTTGCAGA TGTGCAGATC
    TCAGTTGGTT GGATGCACGC AGGCTACCCC ATCATGTGCC ACCTGGAGTC GGTGCAGGAG
    CTCATCAATG AGAAACTCAT CAGAACCAAG GGGCTCTGGG GCCCCGTGCA CGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCCCCGCACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TCTACGTGCA CGAGACGGTC CTGGGCATCC CTCGAGGACG AGCCAACATT
    GCTCTGTGGC CCCCGGTTCG GGAGAAAAGA GTCCGAATTT ACCTGGGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCGTG GACGGCCCTG GAGACGTACT TACAGCTCCA GGAGGCCTTT
    GGGTGGGAGC CGTTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GACAACGTCG ACAAGATGAA TCTGTGGGTG AAAATGTTCT CCCACCAAGT GCAGAAGAAT
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCGATCCAGA AGGAAGTGGC AACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATA ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.