LOC531747 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005192924.3	XP_005192981.1	adenylate cyclase type 10 isoform X3
XM_005223523.3	XP_005223580.2	adenylate cyclase type 10 isoform X2
XM_005223523.4	XP_005223580.2	adenylate cyclase type 10 isoform X3
XM_005223521.3	XP_005223578.2	adenylate cyclase type 10 isoform X1
XM_005223527.3	XP_005223584.2	adenylate cyclase type 10 isoform X4
XM_024983975.1	XP_024839743.1	adenylate cyclase type 10 isoform X2
XM_005223525.3	XP_005223582.1	adenylate cyclase type 10 isoform X3
XM_005192920.3	XP_005192977.2	adenylate cyclase type 10 isoform X1
XM_005192922.3	XP_005192979.2	adenylate cyclase type 10 isoform X2
XM_024983976.1	XP_024839744.1	adenylate cyclase type 10 isoform X4
XM_005223525.4	XP_005223582.1	adenylate cyclase type 10 isoform X5
XM_024983977.1	XP_024839745.1	adenylate cyclase type 10 isoform X6
XM_610247.9	XP_610247.7	adenylate cyclase type 10 isoform X5
XM_005223521.4	XP_005223578.2	adenylate cyclase type 10 isoform X1
XM_005223527.4	XP_005223584.2	adenylate cyclase type 10 isoform X6
XM_005192926.3	XP_005192983.2	adenylate cyclase type 10 isoform X4
XM_002697288.5	XP_002697334.3	adenylate cyclase type 10 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa11215	XM_002697288.5 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa11215	XM_610247.9 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa229728	XM_024983975.1 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa229729	XM_024983976.1 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa11213	XM_005223525.4 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11213	XM_005192924.3 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52049	XM_005223521.4 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52049	XM_005192920.3 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52050	XM_005192922.3 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52050	XM_005223523.4 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52051	XM_005223527.4 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52051	XM_024983977.1 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52051	XM_005192926.3 Latest version!	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa52050	XM_005223523.3	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11213	XM_005223525.3	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52049	XM_005223521.3	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa52051	XM_005223527.3	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

LOC531747 ( XM_610247.9 ) cDNA ORF clone, Bos taurus(cattle) -> XP_610247.7 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa11215
Clone ID Related Accession (Same CDS sequence)	XM_610247.9 , XM_002697288.5
Accession Version	XM_610247.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_610247.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC TTTTGAAGAA GATTGATGAA GGCCAGCCTA TGGAGTATAT ATCCGAATTC 
CGCACTGTGA CTATGGTTTT GGTCAGCCTC GAGTTCCACA AGACAGCATG GGTGTTGCAT 
TTGTGCCATC TTATCCAGGA GGCTGCGTTA TACATCTCCA CAGTGATAGA GACAGGGGGT 
GGCCAGCTGA GCCGGATCTT TATGTTTGAG ACAGGCTGCA TGTTCCTCTG TGTTTTCGGC 
CTACCTGGGG ATAAGAAGCC AGACGAGTGT GCACATGCCC TGGAGAGCTC CTTCAGCATC 
TTCAGCTTCT GCTGGGAGAA TCTTGCTGAG ACCAAGCTTG TTTCCATCAG TATCACCAAT 
GGACCAGTAT TCTGTGGCAT GGTTGGGGCA GTTTCAAGAC ACGAATATAC AGTTATTGGC 
CCAAAAGTAA GCCTCGTGGC AAGAATGATA ACTGCTTATC CAGGTTTGGT GTCCTGTGAT 
GAGGTAACAT ATCTGAGATC TATGCTACCT GCTTACAACT TCAAGAAACT CCCAGAGAAA 
ATGATGAAAA ATATCTCTAA CCCAGGGAAG ATGTATGAAT ACGTTGGCCA TAGAAGATGT 
ATAATGTTTG GGAAGAGACA TTTGGCCACA GAGAGAAATA AAAATCACTC TTTGCTAGAC 
CGGGAGAAAG AACTGGAAGC TTTCCGAATG GCACAGCAGG AATGTTTGCA CCAGAAGAAG 
GGACAGGCAG TTCTATATGA AGGTGGAAAA GGCAGTGGAA AAAGTCAGCT CTTGGCTGAA 
ATAAACTTCC TGGCCCAGAA GGAAGGGCAC AGGGTGTTGC CGTTGAAGCT GAAGAAAGCG 
GATTCCAAGC AGTGCTTCTA CGCCATCCAG ACCCTCATGG CCATCTTCTT CGAGATTGAT 
ACATGCCCAG CCCATTACCG GCAAGAGCGC CTCCACAGAC AGCTCAGTGG AATAGTGGAG 
GAGCCACTTC ACTGCCTTTT TAATGACCTC TTCTTTGTGA AGTTTCCCTT ATCCTTCAAA 
GTTTCACGCA TGGATGACCT GGCCAGACAA CAGAAGATTA AAGCATGTTT TTTTGAAGTG 
CTTGAGAAGG CAGTAAGGGA AACACCACTG ATTTTCCTCA TTGACGAGGC CCAGTATATG 
GATGCAGCTT CCTGGGAATT TTTGGGAACA TTGCTCTCCG GTGTGCCTAT CATCATGGTG 
ATGACGTTGA CCACTACCTC CACCCTGTGT GGCCAGGCCC AGCACTTCCT GCAGAGCCCT 
CCAGCTGTCC TGGTGCCCAT TTCAAAGTTG GCAGCGACCT CACTGTTGAG AATGGCATGT 
TGGGAACTGG GGGTGGTGAG CATCCCCCAA GAGCTGCAGA CCTACCTTCT CCGCAGGTGC 
TTTGGAAACC CCCTTTATTG TGAGGTCTTA TGCCAGGATC TTCTCTCTAA AAACGTATTG 
TGCTTTCATG ACCTCCAAAA GGAGGAAGTG GAGAACAGCA AGTGGGAGAC CCTCTCAGCC 
AATGCCATGA AATCCACCAT GTACAGTGTT TCTCCTGCCA GCTCTGAAGA CCAGGAACTT 
TATGTCTGCA CAGTCAAGGA TGATGTGAAC TTGGATACAG TGCTTCTTCC ACCGTTATTA 
AAAGAAATAG CAGTGAACCA AATAGATCAA CTGAGCCCAG AGGAACAGTC GCTGGTTAAA 
TGTGCTGCAG TCATTGGTCA CTTCTTCCAC ATAGATCTGC TGCAGCACCT CCTGCCTGGC 
TGGAGTAAGA ATAAGCTACT TGAGGTACTG AAGGATCTTG TGGATATGCA CGTGCTCCAC 
TGGTGCAATA AGGGCCAAGA GCTTCCTGCT GAGCCAATAT TAGTGCCTTC CTCTGTCGAG 
ACTGAGCATA CCAAAGAGAC GACAAAGTCA GATGCTGACT CTCCTCTAAA GCTACAAGAG 
GAACTATCCC TACCTCAGAT GGAGGTGCTT GAATTTGGAG TGCCTCTTTT GCGGGAAGGG 
GCTTGGGAGC TGTGGCCCAA GGCACAGCAA ACAGCCCTAC ACCTTGAATG TGCCTGCTTT 
CTTCGAGATC TGGCCTGCCG CTGTGGGAGC TGTCATGGGG GAGACTTTGT CCCCTTCCAC 
CGTTTCGCCA TCTGTTCCAC TAAGAGCTCC AGCAGGACCT CCCGATTCTG TAGCTACAAA 
GACACTGGCT CGATACTAAC ACAAGTGATC ACAGACAGAT TGCAGCTGCC TTCTCCCCAA 
GACACTTTTC AGTTCCAGAC AGAATCATCC AGAAGTCAGG AGGCCTTCAC AAGGGAATAC 
TGCAGAACAG AGGAAGAGTT CCTGGATAAA GTGAACAGGA AACTGGCTCA GACCAACCCC 
AAGAAAGGCA TGTTGACCAC GAAGCCCTGC CACTGTAGGG AAGTCCTGAA GTTAGTGCTC 
TCGCCCCTCA CCCAGCACTG CCTGGTCATT GGAGAAACCA CGTGTGCATT TTATTACCTG 
CTGGAATCTG CCGCTGCCTC CTTGGATCTG TCAGATAATT ACATGGCCTT CTTTTATTTG 
AGGAAGGCAA GCAGTCTTCT GGGCCAACCC TCCGCATTCT CGTTTTTTAA AAAGCACAAG 
GTGAAGATCT GTCAGTTTGA AGAAGCCACT TTCTGTCGTC TCCAGTCAGA GGTCTGTTTC 
AACATGGGAC AGATCACCCT GGCCAAAAAA CTGGCTAGGC GAGCCCTTCG TCTGCTGAAA 
AGGAGTTTTC CCTGGACCTG GCTTGGTGTC ATTTTCCAAA CATTTCTAGA AAAATGCTGG 
TATTCCTGTT CCCTGAGTCA ACCTCGAAAC AACCCTAGTG AGAAGAAGAA GAAGCTGGCA 
GTCCTGCAGC AGCAGATGCA CTGTCTCTCC CTGCTCCGGC AGCTCTACAG CCTGGAGGCC 
ACAGCCAGCA GCCTCAGGTT TGCCTGCCTG GCCACTCTTA TGCAGAAGAA TTCAGTCAAG 
GAGTTTCCTG GTGAAGCCCA GGTTGTCTCT ACCTATGTGG CCCTCTCCCA GTTCTCCCAG 
AACGTGGGTG ACAAGGAGAA GTGGCTGTAC CATGAGCAGC TGGCCATTCA GAAAAGCAGC 
CTGTGCTGGT TCTCCAGGGA GGGACTGTTG GCCACAGCCC AGCTCATGCA GGCCTTGGCC 
TACACCAAAC TCTGCCTTGG GCATCTCGAC TTCTCCATCA AGCTGGGTTT TCAAGCTCAT 
GAGATATGCA GACACCTCAA GAAACCAGCT CTGGAGAACC TGGTTCTCTC AGTTCTCTTC 
AGATCTGCAT TTCTGAAGAA GAAGTTTGAT CTGTGTGTCC ACGTGCTGGA GAGTCAGTGG 
GTGCTCATTT CCCAGAGTCA TGATGTCCTT GGCCGGGCCT TCTTCTATTC TGCTTGCTTA 
GATCTGCTGC TCTACGGAAA AGGATGGCTG TGTCGGCCCT TTGGGGAATG TCTGCATTTC 
ATTCAACGCT ATGAGCACAG CTGCATTCTA AATTCTCAGA GCAGTGTCAT GCTGGGGGTC 
CACTCCTCTC TGGCCATGTG GTTTGCCCAG GACTTACAGT GGAATCAGTT TCAGTACCAC 
TTCTCCAAGG CTCGCCAGTT GGTGAAAAGA ACCAATGCCT CACTATTTGG TTCCCATGGC 
TTTGTCCGAT TCCTAGAGTG CCAGGTGCTA ATTTTACAGA AAATGCCAGA AGATGTCTTC 
CTGCAAACTC TCGCGGAGAC TCGCCGCCAA ACCCTCACGT ACTTTAAGGA GTTTTTCTCT 
CGATGTGTGA CCTGCCCTGT CTATCACCAG TGGGTCTCTG AGCTTAAAGC CTTTGTAATG 
AGAAATGGAA AGATGTCCTT GACCAACATC ATCAGACCTT TTGACACCTG CCTGACCAGG 
ATTTGGATAG AGGGAGAATT CCTGGAGGAA AATAACTCCT TTGAACAAAA TTTGGAAATA 
CAGAGATTTG TCAGAGAGGC TGATGTCAAC AAGAAGGATA AAGAAGAAAT TCAAGAATGT 
ATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_610247.7
CDS	260..4228
Translation	MKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_610247.9
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_610247.9

ORF Insert Sequence:

ATGAAAAACC TTTTGAAGAA GATTGATGAA GGCCAGCCTA TGGAGTATAT ATCCGAATTC 
CGCACTGTGA CTATGGTTTT GGTCAGCCTC GAGTTCCACA AGACAGCATG GGTGTTGCAT 
TTGTGCCATC TTATCCAGGA GGCTGCGTTA TACATCTCCA CAGTGATAGA GACAGGGGGT 
GGCCAGCTGA GCCGGATCTT TATGTTTGAG ACAGGCTGCA TGTTCCTCTG TGTTTTCGGC 
CTACCTGGGG ATAAGAAGCC AGACGAGTGT GCACATGCCC TGGAGAGCTC CTTCAGCATC 
TTCAGCTTCT GCTGGGAGAA TCTTGCTGAG ACCAAGCTTG TTTCCATCAG TATCACCAAT 
GGACCAGTAT TCTGTGGCAT GGTTGGGGCA GTTTCAAGAC ACGAATATAC AGTTATTGGC 
CCAAAAGTAA GCCTCGTGGC AAGAATGATA ACTGCTTATC CAGGTTTGGT GTCCTGTGAT 
GAGGTAACAT ATCTGAGATC TATGCTACCT GCTTACAACT TCAAGAAACT CCCAGAGAAA 
ATGATGAAAA ATATCTCTAA CCCAGGGAAG ATGTATGAAT ACGTTGGCCA TAGAAGATGT 
ATAATGTTTG GGAAGAGACA TTTGGCCACA GAGAGAAATA AAAATCACTC TTTGCTAGAC 
CGGGAGAAAG AACTGGAAGC TTTCCGAATG GCACAGCAGG AATGTTTGCA CCAGAAGAAG 
GGACAGGCAG TTCTATATGA AGGTGGAAAA GGCAGTGGAA AAAGTCAGCT CTTGGCTGAA 
ATAAACTTCC TGGCCCAGAA GGAAGGGCAC AGGGTGTTGC CGTTGAAGCT GAAGAAAGCG 
GATTCCAAGC AGTGCTTCTA CGCCATCCAG ACCCTCATGG CCATCTTCTT CGAGATTGAT 
ACATGCCCAG CCCATTACCG GCAAGAGCGC CTCCACAGAC AGCTCAGTGG AATAGTGGAG 
GAGCCACTTC ACTGCCTTTT TAATGACCTC TTCTTTGTGA AGTTTCCCTT ATCCTTCAAA 
GTTTCACGCA TGGATGACCT GGCCAGACAA CAGAAGATTA AAGCATGTTT TTTTGAAGTG 
CTTGAGAAGG CAGTAAGGGA AACACCACTG ATTTTCCTCA TTGACGAGGC CCAGTATATG 
GATGCAGCTT CCTGGGAATT TTTGGGAACA TTGCTCTCCG GTGTGCCTAT CATCATGGTG 
ATGACGTTGA CCACTACCTC CACCCTGTGT GGCCAGGCCC AGCACTTCCT GCAGAGCCCT 
CCAGCTGTCC TGGTGCCCAT TTCAAAGTTG GCAGCGACCT CACTGTTGAG AATGGCATGT 
TGGGAACTGG GGGTGGTGAG CATCCCCCAA GAGCTGCAGA CCTACCTTCT CCGCAGGTGC 
TTTGGAAACC CCCTTTATTG TGAGGTCTTA TGCCAGGATC TTCTCTCTAA AAACGTATTG 
TGCTTTCATG ACCTCCAAAA GGAGGAAGTG GAGAACAGCA AGTGGGAGAC CCTCTCAGCC 
AATGCCATGA AATCCACCAT GTACAGTGTT TCTCCTGCCA GCTCTGAAGA CCAGGAACTT 
TATGTCTGCA CAGTCAAGGA TGATGTGAAC TTGGATACAG TGCTTCTTCC ACCGTTATTA 
AAAGAAATAG CAGTGAACCA AATAGATCAA CTGAGCCCAG AGGAACAGTC GCTGGTTAAA 
TGTGCTGCAG TCATTGGTCA CTTCTTCCAC ATAGATCTGC TGCAGCACCT CCTGCCTGGC 
TGGAGTAAGA ATAAGCTACT TGAGGTACTG AAGGATCTTG TGGATATGCA CGTGCTCCAC 
TGGTGCAATA AGGGCCAAGA GCTTCCTGCT GAGCCAATAT TAGTGCCTTC CTCTGTCGAG 
ACTGAGCATA CCAAAGAGAC GACAAAGTCA GATGCTGACT CTCCTCTAAA GCTACAAGAG 
GAACTATCCC TACCTCAGAT GGAGGTGCTT GAATTTGGAG TGCCTCTTTT GCGGGAAGGG 
GCTTGGGAGC TGTGGCCCAA GGCACAGCAA ACAGCCCTAC ACCTTGAATG TGCCTGCTTT 
CTTCGAGATC TGGCCTGCCG CTGTGGGAGC TGTCATGGGG GAGACTTTGT CCCCTTCCAC 
CGTTTCGCCA TCTGTTCCAC TAAGAGCTCC AGCAGGACCT CCCGATTCTG TAGCTACAAA 
GACACTGGCT CGATACTAAC ACAAGTGATC ACAGACAGAT TGCAGCTGCC TTCTCCCCAA 
GACACTTTTC AGTTCCAGAC AGAATCATCC AGAAGTCAGG AGGCCTTCAC AAGGGAATAC 
TGCAGAACAG AGGAAGAGTT CCTGGATAAA GTGAACAGGA AACTGGCTCA GACCAACCCC 
AAGAAAGGCA TGTTGACCAC GAAGCCCTGC CACTGTAGGG AAGTCCTGAA GTTAGTGCTC 
TCGCCCCTCA CCCAGCACTG CCTGGTCATT GGAGAAACCA CGTGTGCATT TTATTACCTG 
CTGGAATCTG CCGCTGCCTC CTTGGATCTG TCAGATAATT ACATGGCCTT CTTTTATTTG 
AGGAAGGCAA GCAGTCTTCT GGGCCAACCC TCCGCATTCT CGTTTTTTAA AAAGCACAAG 
GTGAAGATCT GTCAGTTTGA AGAAGCCACT TTCTGTCGTC TCCAGTCAGA GGTCTGTTTC 
AACATGGGAC AGATCACCCT GGCCAAAAAA CTGGCTAGGC GAGCCCTTCG TCTGCTGAAA 
AGGAGTTTTC CCTGGACCTG GCTTGGTGTC ATTTTCCAAA CATTTCTAGA AAAATGCTGG 
TATTCCTGTT CCCTGAGTCA ACCTCGAAAC AACCCTAGTG AGAAGAAGAA GAAGCTGGCA 
GTCCTGCAGC AGCAGATGCA CTGTCTCTCC CTGCTCCGGC AGCTCTACAG CCTGGAGGCC 
ACAGCCAGCA GCCTCAGGTT TGCCTGCCTG GCCACTCTTA TGCAGAAGAA TTCAGTCAAG 
GAGTTTCCTG GTGAAGCCCA GGTTGTCTCT ACCTATGTGG CCCTCTCCCA GTTCTCCCAG 
AACGTGGGTG ACAAGGAGAA GTGGCTGTAC CATGAGCAGC TGGCCATTCA GAAAAGCAGC 
CTGTGCTGGT TCTCCAGGGA GGGACTGTTG GCCACAGCCC AGCTCATGCA GGCCTTGGCC 
TACACCAAAC TCTGCCTTGG GCATCTCGAC TTCTCCATCA AGCTGGGTTT TCAAGCTCAT 
GAGATATGCA GACACCTCAA GAAACCAGCT CTGGAGAACC TGGTTCTCTC AGTTCTCTTC 
AGATCTGCAT TTCTGAAGAA GAAGTTTGAT CTGTGTGTCC ACGTGCTGGA GAGTCAGTGG 
GTGCTCATTT CCCAGAGTCA TGATGTCCTT GGCCGGGCCT TCTTCTATTC TGCTTGCTTA 
GATCTGCTGC TCTACGGAAA AGGATGGCTG TGTCGGCCCT TTGGGGAATG TCTGCATTTC 
ATTCAACGCT ATGAGCACAG CTGCATTCTA AATTCTCAGA GCAGTGTCAT GCTGGGGGTC 
CACTCCTCTC TGGCCATGTG GTTTGCCCAG GACTTACAGT GGAATCAGTT TCAGTACCAC 
TTCTCCAAGG CTCGCCAGTT GGTGAAAAGA ACCAATGCCT CACTATTTGG TTCCCATGGC 
TTTGTCCGAT TCCTAGAGTG CCAGGTGCTA ATTTTACAGA AAATGCCAGA AGATGTCTTC 
CTGCAAACTC TCGCGGAGAC TCGCCGCCAA ACCCTCACGT ACTTTAAGGA GTTTTTCTCT 
CGATGTGTGA CCTGCCCTGT CTATCACCAG TGGGTCTCTG AGCTTAAAGC CTTTGTAATG 
AGAAATGGAA AGATGTCCTT GACCAACATC ATCAGACCTT TTGACACCTG CCTGACCAGG 
ATTTGGATAG AGGGAGAATT CCTGGAGGAA AATAACTCCT TTGAACAAAA TTTGGAAATA 
CAGAGATTTG TCAGAGAGGC TGATGTCAAC AAGAAGGATA AAGAAGAAAT TCAAGAATGT 
ATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_002697288.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697334.3 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa11215
Clone ID Related Accession (Same CDS sequence)	XM_610247.9 , XM_002697288.5
Accession Version	XM_002697288.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697288.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC TTTTGAAGAA GATTGATGAA GGCCAGCCTA TGGAGTATAT ATCCGAATTC 
CGCACTGTGA CTATGGTTTT GGTCAGCCTC GAGTTCCACA AGACAGCATG GGTGTTGCAT 
TTGTGCCATC TTATCCAGGA GGCTGCGTTA TACATCTCCA CAGTGATAGA GACAGGGGGT 
GGCCAGCTGA GCCGGATCTT TATGTTTGAG ACAGGCTGCA TGTTCCTCTG TGTTTTCGGC 
CTACCTGGGG ATAAGAAGCC AGACGAGTGT GCACATGCCC TGGAGAGCTC CTTCAGCATC 
TTCAGCTTCT GCTGGGAGAA TCTTGCTGAG ACCAAGCTTG TTTCCATCAG TATCACCAAT 
GGACCAGTAT TCTGTGGCAT GGTTGGGGCA GTTTCAAGAC ACGAATATAC AGTTATTGGC 
CCAAAAGTAA GCCTCGTGGC AAGAATGATA ACTGCTTATC CAGGTTTGGT GTCCTGTGAT 
GAGGTAACAT ATCTGAGATC TATGCTACCT GCTTACAACT TCAAGAAACT CCCAGAGAAA 
ATGATGAAAA ATATCTCTAA CCCAGGGAAG ATGTATGAAT ACGTTGGCCA TAGAAGATGT 
ATAATGTTTG GGAAGAGACA TTTGGCCACA GAGAGAAATA AAAATCACTC TTTGCTAGAC 
CGGGAGAAAG AACTGGAAGC TTTCCGAATG GCACAGCAGG AATGTTTGCA CCAGAAGAAG 
GGACAGGCAG TTCTATATGA AGGTGGAAAA GGCAGTGGAA AAAGTCAGCT CTTGGCTGAA 
ATAAACTTCC TGGCCCAGAA GGAAGGGCAC AGGGTGTTGC CGTTGAAGCT GAAGAAAGCG 
GATTCCAAGC AGTGCTTCTA CGCCATCCAG ACCCTCATGG CCATCTTCTT CGAGATTGAT 
ACATGCCCAG CCCATTACCG GCAAGAGCGC CTCCACAGAC AGCTCAGTGG AATAGTGGAG 
GAGCCACTTC ACTGCCTTTT TAATGACCTC TTCTTTGTGA AGTTTCCCTT ATCCTTCAAA 
GTTTCACGCA TGGATGACCT GGCCAGACAA CAGAAGATTA AAGCATGTTT TTTTGAAGTG 
CTTGAGAAGG CAGTAAGGGA AACACCACTG ATTTTCCTCA TTGACGAGGC CCAGTATATG 
GATGCAGCTT CCTGGGAATT TTTGGGAACA TTGCTCTCCG GTGTGCCTAT CATCATGGTG 
ATGACGTTGA CCACTACCTC CACCCTGTGT GGCCAGGCCC AGCACTTCCT GCAGAGCCCT 
CCAGCTGTCC TGGTGCCCAT TTCAAAGTTG GCAGCGACCT CACTGTTGAG AATGGCATGT 
TGGGAACTGG GGGTGGTGAG CATCCCCCAA GAGCTGCAGA CCTACCTTCT CCGCAGGTGC 
TTTGGAAACC CCCTTTATTG TGAGGTCTTA TGCCAGGATC TTCTCTCTAA AAACGTATTG 
TGCTTTCATG ACCTCCAAAA GGAGGAAGTG GAGAACAGCA AGTGGGAGAC CCTCTCAGCC 
AATGCCATGA AATCCACCAT GTACAGTGTT TCTCCTGCCA GCTCTGAAGA CCAGGAACTT 
TATGTCTGCA CAGTCAAGGA TGATGTGAAC TTGGATACAG TGCTTCTTCC ACCGTTATTA 
AAAGAAATAG CAGTGAACCA AATAGATCAA CTGAGCCCAG AGGAACAGTC GCTGGTTAAA 
TGTGCTGCAG TCATTGGTCA CTTCTTCCAC ATAGATCTGC TGCAGCACCT CCTGCCTGGC 
TGGAGTAAGA ATAAGCTACT TGAGGTACTG AAGGATCTTG TGGATATGCA CGTGCTCCAC 
TGGTGCAATA AGGGCCAAGA GCTTCCTGCT GAGCCAATAT TAGTGCCTTC CTCTGTCGAG 
ACTGAGCATA CCAAAGAGAC GACAAAGTCA GATGCTGACT CTCCTCTAAA GCTACAAGAG 
GAACTATCCC TACCTCAGAT GGAGGTGCTT GAATTTGGAG TGCCTCTTTT GCGGGAAGGG 
GCTTGGGAGC TGTGGCCCAA GGCACAGCAA ACAGCCCTAC ACCTTGAATG TGCCTGCTTT 
CTTCGAGATC TGGCCTGCCG CTGTGGGAGC TGTCATGGGG GAGACTTTGT CCCCTTCCAC 
CGTTTCGCCA TCTGTTCCAC TAAGAGCTCC AGCAGGACCT CCCGATTCTG TAGCTACAAA 
GACACTGGCT CGATACTAAC ACAAGTGATC ACAGACAGAT TGCAGCTGCC TTCTCCCCAA 
GACACTTTTC AGTTCCAGAC AGAATCATCC AGAAGTCAGG AGGCCTTCAC AAGGGAATAC 
TGCAGAACAG AGGAAGAGTT CCTGGATAAA GTGAACAGGA AACTGGCTCA GACCAACCCC 
AAGAAAGGCA TGTTGACCAC GAAGCCCTGC CACTGTAGGG AAGTCCTGAA GTTAGTGCTC 
TCGCCCCTCA CCCAGCACTG CCTGGTCATT GGAGAAACCA CGTGTGCATT TTATTACCTG 
CTGGAATCTG CCGCTGCCTC CTTGGATCTG TCAGATAATT ACATGGCCTT CTTTTATTTG 
AGGAAGGCAA GCAGTCTTCT GGGCCAACCC TCCGCATTCT CGTTTTTTAA AAAGCACAAG 
GTGAAGATCT GTCAGTTTGA AGAAGCCACT TTCTGTCGTC TCCAGTCAGA GGTCTGTTTC 
AACATGGGAC AGATCACCCT GGCCAAAAAA CTGGCTAGGC GAGCCCTTCG TCTGCTGAAA 
AGGAGTTTTC CCTGGACCTG GCTTGGTGTC ATTTTCCAAA CATTTCTAGA AAAATGCTGG 
TATTCCTGTT CCCTGAGTCA ACCTCGAAAC AACCCTAGTG AGAAGAAGAA GAAGCTGGCA 
GTCCTGCAGC AGCAGATGCA CTGTCTCTCC CTGCTCCGGC AGCTCTACAG CCTGGAGGCC 
ACAGCCAGCA GCCTCAGGTT TGCCTGCCTG GCCACTCTTA TGCAGAAGAA TTCAGTCAAG 
GAGTTTCCTG GTGAAGCCCA GGTTGTCTCT ACCTATGTGG CCCTCTCCCA GTTCTCCCAG 
AACGTGGGTG ACAAGGAGAA GTGGCTGTAC CATGAGCAGC TGGCCATTCA GAAAAGCAGC 
CTGTGCTGGT TCTCCAGGGA GGGACTGTTG GCCACAGCCC AGCTCATGCA GGCCTTGGCC 
TACACCAAAC TCTGCCTTGG GCATCTCGAC TTCTCCATCA AGCTGGGTTT TCAAGCTCAT 
GAGATATGCA GACACCTCAA GAAACCAGCT CTGGAGAACC TGGTTCTCTC AGTTCTCTTC 
AGATCTGCAT TTCTGAAGAA GAAGTTTGAT CTGTGTGTCC ACGTGCTGGA GAGTCAGTGG 
GTGCTCATTT CCCAGAGTCA TGATGTCCTT GGCCGGGCCT TCTTCTATTC TGCTTGCTTA 
GATCTGCTGC TCTACGGAAA AGGATGGCTG TGTCGGCCCT TTGGGGAATG TCTGCATTTC 
ATTCAACGCT ATGAGCACAG CTGCATTCTA AATTCTCAGA GCAGTGTCAT GCTGGGGGTC 
CACTCCTCTC TGGCCATGTG GTTTGCCCAG GACTTACAGT GGAATCAGTT TCAGTACCAC 
TTCTCCAAGG CTCGCCAGTT GGTGAAAAGA ACCAATGCCT CACTATTTGG TTCCCATGGC 
TTTGTCCGAT TCCTAGAGTG CCAGGTGCTA ATTTTACAGA AAATGCCAGA AGATGTCTTC 
CTGCAAACTC TCGCGGAGAC TCGCCGCCAA ACCCTCACGT ACTTTAAGGA GTTTTTCTCT 
CGATGTGTGA CCTGCCCTGT CTATCACCAG TGGGTCTCTG AGCTTAAAGC CTTTGTAATG 
AGAAATGGAA AGATGTCCTT GACCAACATC ATCAGACCTT TTGACACCTG CCTGACCAGG 
ATTTGGATAG AGGGAGAATT CCTGGAGGAA AATAACTCCT TTGAACAAAA TTTGGAAATA 
CAGAGATTTG TCAGAGAGGC TGATGTCAAC AAGAAGGATA AAGAAGAAAT TCAAGAATGT 
ATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697334.3
CDS	260..4228
Translation	MKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_002697288.5
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697288.5

ORF Insert Sequence:

ATGAAAAACC TTTTGAAGAA GATTGATGAA GGCCAGCCTA TGGAGTATAT ATCCGAATTC 
CGCACTGTGA CTATGGTTTT GGTCAGCCTC GAGTTCCACA AGACAGCATG GGTGTTGCAT 
TTGTGCCATC TTATCCAGGA GGCTGCGTTA TACATCTCCA CAGTGATAGA GACAGGGGGT 
GGCCAGCTGA GCCGGATCTT TATGTTTGAG ACAGGCTGCA TGTTCCTCTG TGTTTTCGGC 
CTACCTGGGG ATAAGAAGCC AGACGAGTGT GCACATGCCC TGGAGAGCTC CTTCAGCATC 
TTCAGCTTCT GCTGGGAGAA TCTTGCTGAG ACCAAGCTTG TTTCCATCAG TATCACCAAT 
GGACCAGTAT TCTGTGGCAT GGTTGGGGCA GTTTCAAGAC ACGAATATAC AGTTATTGGC 
CCAAAAGTAA GCCTCGTGGC AAGAATGATA ACTGCTTATC CAGGTTTGGT GTCCTGTGAT 
GAGGTAACAT ATCTGAGATC TATGCTACCT GCTTACAACT TCAAGAAACT CCCAGAGAAA 
ATGATGAAAA ATATCTCTAA CCCAGGGAAG ATGTATGAAT ACGTTGGCCA TAGAAGATGT 
ATAATGTTTG GGAAGAGACA TTTGGCCACA GAGAGAAATA AAAATCACTC TTTGCTAGAC 
CGGGAGAAAG AACTGGAAGC TTTCCGAATG GCACAGCAGG AATGTTTGCA CCAGAAGAAG 
GGACAGGCAG TTCTATATGA AGGTGGAAAA GGCAGTGGAA AAAGTCAGCT CTTGGCTGAA 
ATAAACTTCC TGGCCCAGAA GGAAGGGCAC AGGGTGTTGC CGTTGAAGCT GAAGAAAGCG 
GATTCCAAGC AGTGCTTCTA CGCCATCCAG ACCCTCATGG CCATCTTCTT CGAGATTGAT 
ACATGCCCAG CCCATTACCG GCAAGAGCGC CTCCACAGAC AGCTCAGTGG AATAGTGGAG 
GAGCCACTTC ACTGCCTTTT TAATGACCTC TTCTTTGTGA AGTTTCCCTT ATCCTTCAAA 
GTTTCACGCA TGGATGACCT GGCCAGACAA CAGAAGATTA AAGCATGTTT TTTTGAAGTG 
CTTGAGAAGG CAGTAAGGGA AACACCACTG ATTTTCCTCA TTGACGAGGC CCAGTATATG 
GATGCAGCTT CCTGGGAATT TTTGGGAACA TTGCTCTCCG GTGTGCCTAT CATCATGGTG 
ATGACGTTGA CCACTACCTC CACCCTGTGT GGCCAGGCCC AGCACTTCCT GCAGAGCCCT 
CCAGCTGTCC TGGTGCCCAT TTCAAAGTTG GCAGCGACCT CACTGTTGAG AATGGCATGT 
TGGGAACTGG GGGTGGTGAG CATCCCCCAA GAGCTGCAGA CCTACCTTCT CCGCAGGTGC 
TTTGGAAACC CCCTTTATTG TGAGGTCTTA TGCCAGGATC TTCTCTCTAA AAACGTATTG 
TGCTTTCATG ACCTCCAAAA GGAGGAAGTG GAGAACAGCA AGTGGGAGAC CCTCTCAGCC 
AATGCCATGA AATCCACCAT GTACAGTGTT TCTCCTGCCA GCTCTGAAGA CCAGGAACTT 
TATGTCTGCA CAGTCAAGGA TGATGTGAAC TTGGATACAG TGCTTCTTCC ACCGTTATTA 
AAAGAAATAG CAGTGAACCA AATAGATCAA CTGAGCCCAG AGGAACAGTC GCTGGTTAAA 
TGTGCTGCAG TCATTGGTCA CTTCTTCCAC ATAGATCTGC TGCAGCACCT CCTGCCTGGC 
TGGAGTAAGA ATAAGCTACT TGAGGTACTG AAGGATCTTG TGGATATGCA CGTGCTCCAC 
TGGTGCAATA AGGGCCAAGA GCTTCCTGCT GAGCCAATAT TAGTGCCTTC CTCTGTCGAG 
ACTGAGCATA CCAAAGAGAC GACAAAGTCA GATGCTGACT CTCCTCTAAA GCTACAAGAG 
GAACTATCCC TACCTCAGAT GGAGGTGCTT GAATTTGGAG TGCCTCTTTT GCGGGAAGGG 
GCTTGGGAGC TGTGGCCCAA GGCACAGCAA ACAGCCCTAC ACCTTGAATG TGCCTGCTTT 
CTTCGAGATC TGGCCTGCCG CTGTGGGAGC TGTCATGGGG GAGACTTTGT CCCCTTCCAC 
CGTTTCGCCA TCTGTTCCAC TAAGAGCTCC AGCAGGACCT CCCGATTCTG TAGCTACAAA 
GACACTGGCT CGATACTAAC ACAAGTGATC ACAGACAGAT TGCAGCTGCC TTCTCCCCAA 
GACACTTTTC AGTTCCAGAC AGAATCATCC AGAAGTCAGG AGGCCTTCAC AAGGGAATAC 
TGCAGAACAG AGGAAGAGTT CCTGGATAAA GTGAACAGGA AACTGGCTCA GACCAACCCC 
AAGAAAGGCA TGTTGACCAC GAAGCCCTGC CACTGTAGGG AAGTCCTGAA GTTAGTGCTC 
TCGCCCCTCA CCCAGCACTG CCTGGTCATT GGAGAAACCA CGTGTGCATT TTATTACCTG 
CTGGAATCTG CCGCTGCCTC CTTGGATCTG TCAGATAATT ACATGGCCTT CTTTTATTTG 
AGGAAGGCAA GCAGTCTTCT GGGCCAACCC TCCGCATTCT CGTTTTTTAA AAAGCACAAG 
GTGAAGATCT GTCAGTTTGA AGAAGCCACT TTCTGTCGTC TCCAGTCAGA GGTCTGTTTC 
AACATGGGAC AGATCACCCT GGCCAAAAAA CTGGCTAGGC GAGCCCTTCG TCTGCTGAAA 
AGGAGTTTTC CCTGGACCTG GCTTGGTGTC ATTTTCCAAA CATTTCTAGA AAAATGCTGG 
TATTCCTGTT CCCTGAGTCA ACCTCGAAAC AACCCTAGTG AGAAGAAGAA GAAGCTGGCA 
GTCCTGCAGC AGCAGATGCA CTGTCTCTCC CTGCTCCGGC AGCTCTACAG CCTGGAGGCC 
ACAGCCAGCA GCCTCAGGTT TGCCTGCCTG GCCACTCTTA TGCAGAAGAA TTCAGTCAAG 
GAGTTTCCTG GTGAAGCCCA GGTTGTCTCT ACCTATGTGG CCCTCTCCCA GTTCTCCCAG 
AACGTGGGTG ACAAGGAGAA GTGGCTGTAC CATGAGCAGC TGGCCATTCA GAAAAGCAGC 
CTGTGCTGGT TCTCCAGGGA GGGACTGTTG GCCACAGCCC AGCTCATGCA GGCCTTGGCC 
TACACCAAAC TCTGCCTTGG GCATCTCGAC TTCTCCATCA AGCTGGGTTT TCAAGCTCAT 
GAGATATGCA GACACCTCAA GAAACCAGCT CTGGAGAACC TGGTTCTCTC AGTTCTCTTC 
AGATCTGCAT TTCTGAAGAA GAAGTTTGAT CTGTGTGTCC ACGTGCTGGA GAGTCAGTGG 
GTGCTCATTT CCCAGAGTCA TGATGTCCTT GGCCGGGCCT TCTTCTATTC TGCTTGCTTA 
GATCTGCTGC TCTACGGAAA AGGATGGCTG TGTCGGCCCT TTGGGGAATG TCTGCATTTC 
ATTCAACGCT ATGAGCACAG CTGCATTCTA AATTCTCAGA GCAGTGTCAT GCTGGGGGTC 
CACTCCTCTC TGGCCATGTG GTTTGCCCAG GACTTACAGT GGAATCAGTT TCAGTACCAC 
TTCTCCAAGG CTCGCCAGTT GGTGAAAAGA ACCAATGCCT CACTATTTGG TTCCCATGGC 
TTTGTCCGAT TCCTAGAGTG CCAGGTGCTA ATTTTACAGA AAATGCCAGA AGATGTCTTC 
CTGCAAACTC TCGCGGAGAC TCGCCGCCAA ACCCTCACGT ACTTTAAGGA GTTTTTCTCT 
CGATGTGTGA CCTGCCCTGT CTATCACCAG TGGGTCTCTG AGCTTAAAGC CTTTGTAATG 
AGAAATGGAA AGATGTCCTT GACCAACATC ATCAGACCTT TTGACACCTG CCTGACCAGG 
ATTTGGATAG AGGGAGAATT CCTGGAGGAA AATAACTCCT TTGAACAAAA TTTGGAAATA 
CAGAGATTTG TCAGAGAGGC TGATGTCAAC AAGAAGGATA AAGAAGAAAT TCAAGAATGT 
ATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_024983975.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839743.1 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.

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CloneID	OBa229728
Clone ID Related Accession (Same CDS sequence)	XM_024983975.1
Accession Version	XM_024983975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GCACAAGGTG AAGATCTGTC AGTTTGAAGA AGCCACTTTC 
TGTCGTCTCC AGTCAGAGGT CTGTTTCAAC ATGGGACAGA TCACCCTGGC CAAAAAACTG 
GCTAGGCGAG CCCTTCGTCT GCTGAAAAGG AGTTTTCCCT GGACCTGGCT TGGTGTCATT 
TTCCAAACAT TTCTAGAAAA ATGCTGGTAT TCCTGTTCCC TGAGTCAACC TCGAAACAAC 
CCTAGTGAGA AGAAGAAGAA GCTGGCAGTC CTGCAGCAGC AGATGCACTG TCTCTCCCTG 
CTCCGGCAGC TCTACAGCCT GGAGGCCACA GCCAGCAGCC TCAGGTTTGC CTGCCTGGCC 
ACTCTTATGC AGAAGAATTC AGTCAAGGAG TTTCCTGGTG AAGCCCAGGT TGTCTCTACC 
TATGTGGCCC TCTCCCAGTT CTCCCAGAAC GTGGGTGACA AGGAGAAGTG GCTGTACCAT 
GAGCAGCTGG CCATTCAGAA AAGCAGCCTG TGCTGGTTCT CCAGGGAGGG ACTGTTGGCC 
ACAGCCCAGC TCATGCAGGC CTTGGCCTAC ACCAAACTCT GCCTTGGGCA TCTCGACTTC 
TCCATCAAGC TGGGTTTTCA AGCTCATGAG ATATGCAGAC ACCTCAAGAA ACCAGCTCTG 
GAGAACCTGG TTCTCTCAGT TCTCTTCAGA TCTGCATTTC TGAAGAAGAA GTTTGATCTG 
TGTGTCCACG TGCTGGAGAG TCAGTGGGTG CTCATTTCCC AGAGTCATGA TGTCCTTGGC 
CGGGCCTTCT TCTATTCTGC TTGCTTAGAT CTGCTGCTCT ACGGAAAAGG ATGGCTGTGT 
CGGCCCTTTG GGGAATGTCT GCATTTCATT CAACGCTATG AGCACAGCTG CATTCTAAAT 
TCTCAGAGCA GTGTCATGCT GGGGGTCCAC TCCTCTCTGG CCATGTGGTT TGCCCAGGAC 
TTACAGTGGA ATCAGTTTCA GTACCACTTC TCCAAGGCTC GCCAGTTGGT GAAAAGAACC 
AATGCCTCAC TATTTGGTTC CCATGGCTTT GTCCGATTCC TAGAGTGCCA GGTGCTAATT 
TTACAGAAAA TGCCAGAAGA TGTCTTCCTG CAAACTCTCG CGGAGACTCG CCGCCAAACC 
CTCACGTACT TTAAGGAGTT TTTCTCTCGA TGTGTGACCT GCCCTGTCTA TCACCAGTGG 
GTCTCTGAGC TTAAAGCCTT TGTAATGAGA AATGGAAAGA TGTCCTTGAC CAACATCATC 
AGACCTTTTG ACACCTGCCT GACCAGGATT TGGATAGAGG GAGAATTCCT GGAGGAAAAT 
AACTCCTTTG AACAAAATTT GGAAATACAG AGATTTGTCA GAGAGGCTGA TGTCAACAAG 
AAGGATAAAG AAGAAATTCA AGAATGTATC TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839743.1
CDS	711..5546
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_024983975.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983975.1

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GCACAAGGTG AAGATCTGTC AGTTTGAAGA AGCCACTTTC 
TGTCGTCTCC AGTCAGAGGT CTGTTTCAAC ATGGGACAGA TCACCCTGGC CAAAAAACTG 
GCTAGGCGAG CCCTTCGTCT GCTGAAAAGG AGTTTTCCCT GGACCTGGCT TGGTGTCATT 
TTCCAAACAT TTCTAGAAAA ATGCTGGTAT TCCTGTTCCC TGAGTCAACC TCGAAACAAC 
CCTAGTGAGA AGAAGAAGAA GCTGGCAGTC CTGCAGCAGC AGATGCACTG TCTCTCCCTG 
CTCCGGCAGC TCTACAGCCT GGAGGCCACA GCCAGCAGCC TCAGGTTTGC CTGCCTGGCC 
ACTCTTATGC AGAAGAATTC AGTCAAGGAG TTTCCTGGTG AAGCCCAGGT TGTCTCTACC 
TATGTGGCCC TCTCCCAGTT CTCCCAGAAC GTGGGTGACA AGGAGAAGTG GCTGTACCAT 
GAGCAGCTGG CCATTCAGAA AAGCAGCCTG TGCTGGTTCT CCAGGGAGGG ACTGTTGGCC 
ACAGCCCAGC TCATGCAGGC CTTGGCCTAC ACCAAACTCT GCCTTGGGCA TCTCGACTTC 
TCCATCAAGC TGGGTTTTCA AGCTCATGAG ATATGCAGAC ACCTCAAGAA ACCAGCTCTG 
GAGAACCTGG TTCTCTCAGT TCTCTTCAGA TCTGCATTTC TGAAGAAGAA GTTTGATCTG 
TGTGTCCACG TGCTGGAGAG TCAGTGGGTG CTCATTTCCC AGAGTCATGA TGTCCTTGGC 
CGGGCCTTCT TCTATTCTGC TTGCTTAGAT CTGCTGCTCT ACGGAAAAGG ATGGCTGTGT 
CGGCCCTTTG GGGAATGTCT GCATTTCATT CAACGCTATG AGCACAGCTG CATTCTAAAT 
TCTCAGAGCA GTGTCATGCT GGGGGTCCAC TCCTCTCTGG CCATGTGGTT TGCCCAGGAC 
TTACAGTGGA ATCAGTTTCA GTACCACTTC TCCAAGGCTC GCCAGTTGGT GAAAAGAACC 
AATGCCTCAC TATTTGGTTC CCATGGCTTT GTCCGATTCC TAGAGTGCCA GGTGCTAATT 
TTACAGAAAA TGCCAGAAGA TGTCTTCCTG CAAACTCTCG CGGAGACTCG CCGCCAAACC 
CTCACGTACT TTAAGGAGTT TTTCTCTCGA TGTGTGACCT GCCCTGTCTA TCACCAGTGG 
GTCTCTGAGC TTAAAGCCTT TGTAATGAGA AATGGAAAGA TGTCCTTGAC CAACATCATC 
AGACCTTTTG ACACCTGCCT GACCAGGATT TGGATAGAGG GAGAATTCCT GGAGGAAAAT 
AACTCCTTTG AACAAAATTT GGAAATACAG AGATTTGTCA GAGAGGCTGA TGTCAACAAG 
AAGGATAAAG AAGAAATTCA AGAATGTATC TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_024983976.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839744.1 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.

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CloneID	OBa229729
Clone ID Related Accession (Same CDS sequence)	XM_024983976.1
Accession Version	XM_024983976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGTCTGTTTC AACATGGGAC AGATCACCCT GGCCAAAAAA 
CTGGCTAGGC GAGCCCTTCG TCTGCTGAAA AGGAGTTTTC CCTGGACCTG GCTTGGTGTC 
ATTTTCCAAA CATTTCTAGA AAAATGCTGG TATTCCTGTT CCCTGAGTCA ACCTCGAAAC 
AACCCTAGTG AGAAGAAGAA GAAGCTGGCA GTCCTGCAGC AGCAGATGCA CTGTCTCTCC 
CTGCTCCGGC AGCTCTACAG CCTGGAGGCC ACAGCCAGCA GCCTCAGGTT TGCCTGCCTG 
GCCACTCTTA TGCAGAAGAA TTCAGTCAAG GAGTTTCCTG GTGAAGCCCA GGTTGTCTCT 
ACCTATGTGG CCCTCTCCCA GTTCTCCCAG AACGTGGGTG ACAAGGAGAA GTGGCTGTAC 
CATGAGCAGC TGGCCATTCA GAAAAGCAGC CTGTGCTGGT TCTCCAGGGA GGGACTGTTG 
GCCACAGCCC AGCTCATGCA GGCCTTGGCC TACACCAAAC TCTGCCTTGG GCATCTCGAC 
TTCTCCATCA AGCTGGGTTT TCAAGCTCAT GAGATATGCA GACACCTCAA GAAACCAGCT 
CTGGAGAACC TGGTTCTCTC AGTTCTCTTC AGATCTGCAT TTCTGAAGAA GAAGTTTGAT 
CTGTGTGTCC ACGTGCTGGA GAGTCAGTGG GTGCTCATTT CCCAGAGTCA TGATGTCCTT 
GGCCGGGCCT TCTTCTATTC TGCTTGCTTA GATCTGCTGC TCTACGGAAA AGGATGGCTG 
TGTCGGCCCT TTGGGGAATG TCTGCATTTC ATTCAACGCT ATGAGCACAG CTGCATTCTA 
AATTCTCAGA GCAGTGTCAT GCTGGGGGTC CACTCCTCTC TGGCCATGTG GTTTGCCCAG 
GACTTACAGT GGAATCAGTT TCAGTACCAC TTCTCCAAGG CTCGCCAGTT GGTGAAAAGA 
ACCAATGCCT CACTATTTGG TTCCCATGGC TTTGTCCGAT TCCTAGAGTG CCAGGTGCTA 
ATTTTACAGA AAATGCCAGA AGATGTCTTC CTGCAAACTC TCGCGGAGAC TCGCCGCCAA 
ACCCTCACGT ACTTTAAGGA GTTTTTCTCT CGATGTGTGA CCTGCCCTGT CTATCACCAG 
TGGGTCTCTG AGCTTAAAGC CTTTGTAATG AGAAATGGAA AGATGTCCTT GACCAACATC 
ATCAGACCTT TTGACACCTG CCTGACCAGG ATTTGGATAG AGGGAGAATT CCTGGAGGAA 
AATAACTCCT TTGAACAAAA TTTGGAAATA CAGAGATTTG TCAGAGAGGC TGATGTCAAC 
AAGAAGGATA AAGAAGAAAT TCAAGAATGT ATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839744.1
CDS	711..5489
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_024983976.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983976.1

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGTCTGTTTC AACATGGGAC AGATCACCCT GGCCAAAAAA 
CTGGCTAGGC GAGCCCTTCG TCTGCTGAAA AGGAGTTTTC CCTGGACCTG GCTTGGTGTC 
ATTTTCCAAA CATTTCTAGA AAAATGCTGG TATTCCTGTT CCCTGAGTCA ACCTCGAAAC 
AACCCTAGTG AGAAGAAGAA GAAGCTGGCA GTCCTGCAGC AGCAGATGCA CTGTCTCTCC 
CTGCTCCGGC AGCTCTACAG CCTGGAGGCC ACAGCCAGCA GCCTCAGGTT TGCCTGCCTG 
GCCACTCTTA TGCAGAAGAA TTCAGTCAAG GAGTTTCCTG GTGAAGCCCA GGTTGTCTCT 
ACCTATGTGG CCCTCTCCCA GTTCTCCCAG AACGTGGGTG ACAAGGAGAA GTGGCTGTAC 
CATGAGCAGC TGGCCATTCA GAAAAGCAGC CTGTGCTGGT TCTCCAGGGA GGGACTGTTG 
GCCACAGCCC AGCTCATGCA GGCCTTGGCC TACACCAAAC TCTGCCTTGG GCATCTCGAC 
TTCTCCATCA AGCTGGGTTT TCAAGCTCAT GAGATATGCA GACACCTCAA GAAACCAGCT 
CTGGAGAACC TGGTTCTCTC AGTTCTCTTC AGATCTGCAT TTCTGAAGAA GAAGTTTGAT 
CTGTGTGTCC ACGTGCTGGA GAGTCAGTGG GTGCTCATTT CCCAGAGTCA TGATGTCCTT 
GGCCGGGCCT TCTTCTATTC TGCTTGCTTA GATCTGCTGC TCTACGGAAA AGGATGGCTG 
TGTCGGCCCT TTGGGGAATG TCTGCATTTC ATTCAACGCT ATGAGCACAG CTGCATTCTA 
AATTCTCAGA GCAGTGTCAT GCTGGGGGTC CACTCCTCTC TGGCCATGTG GTTTGCCCAG 
GACTTACAGT GGAATCAGTT TCAGTACCAC TTCTCCAAGG CTCGCCAGTT GGTGAAAAGA 
ACCAATGCCT CACTATTTGG TTCCCATGGC TTTGTCCGAT TCCTAGAGTG CCAGGTGCTA 
ATTTTACAGA AAATGCCAGA AGATGTCTTC CTGCAAACTC TCGCGGAGAC TCGCCGCCAA 
ACCCTCACGT ACTTTAAGGA GTTTTTCTCT CGATGTGTGA CCTGCCCTGT CTATCACCAG 
TGGGTCTCTG AGCTTAAAGC CTTTGTAATG AGAAATGGAA AGATGTCCTT GACCAACATC 
ATCAGACCTT TTGACACCTG CCTGACCAGG ATTTGGATAG AGGGAGAATT CCTGGAGGAA 
AATAACTCCT TTGAACAAAA TTTGGAAATA CAGAGATTTG TCAGAGAGGC TGATGTCAAC 
AAGAAGGATA AAGAAGAAAT TCAAGAATGT ATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005192924.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005192981.1 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.

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CloneID	OBa11213
Clone ID Related Accession (Same CDS sequence)	XM_005192924.3 , XM_005223525.3 , XM_005223525.4
Accession Version	XM_005192924.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT TCAGCCTGAG CTGCAAGTTG GAACATGACA TGGACAGACT GGCCCACATC 
TTCAGTTATT ACATTAGTGA CATCGTGGAG CATGTGCTCT GTTTTGGAGG AGATATCCTA 
AATATCACAG GGAATGTGCT CCTAGCACTC TGGACAGTGG AACAAAATCA ACTGAGTGAC 
ATCATTACTC TGGTGGCAAA ATGCAGTCTG GAGATACAGG AGAAATTTGG GATCTACCAT 
ACCAGAGAAG GCCAGGACCT GCAGCTAAAG ATAGGTCTAT CTGCAGGTCG GATTTCCCAG 
GTTATTGTTG GAGATGAGCA GCGCCAGTAC CTCTTGGTGA TTGGCCGGGA TGTAGATGGT 
GTGCAGTTAG CTCAACGGTT GGCTCAAGCA AATGAGATTG TCCTGTCCTG GAACTGCTGG 
AAGCTCTGTG AGCAGTACAT GTTTGAAGTG GAAATCATGC GGGAGGATGA AGCTGTGAAG 
TTAAGAGATA TGCGGATCAT TGACCCATTT GATTTTGATG AGCATTTTTA CAAGTGCCTC 
GATTATCTGC CACATTACCG GACAAGTGCT GACATTCTAA GAGCCGCCCT GGAGTTGGAT 
CCAGATTCTG CACTTGAGCA AACCCTGCGG AAGCACATAA TGAAAAACCT TTTGAAGAAG 
ATTGATGAAG GCCAGCCTAT GGAGTATATA TCCGAATTCC GCACTGTGAC TATGGTTTTG 
GTCAGCCTCG AGTTCCACAA GACAGCATGG GTGTTGCATT TGTGCCATCT TATCCAGGAG 
GCTGCGTTAT ACATCTCCAC AGTGATAGAG ACAGGGGGTG GCCAGCTGAG CCGGATCTTT 
ATGTTTGAGA CAGGCTGCAT GTTCCTCTGT GTTTTCGGCC TACCTGGGGA TAAGAAGCCA 
GACGAGTGTG CACATGCCCT GGAGAGCTCC TTCAGCATCT TCAGCTTCTG CTGGGAGAAT 
CTTGCTGAGA CCAAGCTTGT TTCCATCAGT ATCACCAATG GACCAGTATT CTGTGGCATG 
GTTGGGGCAG TTTCAAGACA CGAATATACA GTTATTGGCC CAAAAGTAAG CCTCGTGGCA 
AGAATGATAA CTGCTTATCC AGGTTTGGTG TCCTGTGATG AGGTAACATA TCTGAGATCT 
ATGCTACCTG CTTACAACTT CAAGAAACTC CCAGAGAAAA TGATGAAAAA TATCTCTAAC 
CCAGGGAAGA TGTATGAATA CGTTGGCCAT AGAAGATGTA TAATGTTTGG GAAGAGACAT 
TTGGCCACAG AGAGAAATAA AAATCACTCT TTGCTAGACC GGGAGAAAGA ACTGGAAGCT 
TTCCGAATGG CACAGCAGGA ATGTTTGCAC CAGAAGAAGG GACAGGCAGT TCTATATGAA 
GGTGGAAAAG GCAGTGGAAA AAGTCAGCTC TTGGCTGAAA TAAACTTCCT GGCCCAGAAG 
GAAGGGCACA GGGTGTTGCC GTTGAAGCTG AAGAAAGCGG ATTCCAAGCA GTGCTTCTAC 
GCCATCCAGA CCCTCATGGC CATCTTCTTC GAGATTGATA CATGCCCAGC CCATTACCGG 
CAAGAGCGCC TCCACAGACA GCTCAGTGGA ATAGTGGAGG AGCCACTTCA CTGCCTTTTT 
AATGACCTCT TCTTTGTGAA GTTTCCCTTA TCCTTCAAAG TTTCACGCAT GGATGACCTG 
GCCAGACAAC AGAAGATTAA AGCATGTTTT TTTGAAGTGC TTGAGAAGGC AGTAAGGGAA 
ACACCACTGA TTTTCCTCAT TGACGAGGCC CAGTATATGG ATGCAGCTTC CTGGGAATTT 
TTGGGAACAT TGCTCTCCGG TGTGCCTATC ATCATGGTGA TGACGTTGAC CACTACCTCC 
ACCCTGTGTG GCCAGGCCCA GCACTTCCTG CAGAGCCCTC CAGCTGTCCT GGTGCCCATT 
TCAAAGTTGG CAGCGACCTC ACTGTTGAGA ATGGCATGTT GGGAACTGGG GGTGGTGAGC 
ATCCCCCAAG AGCTGCAGAC CTACCTTCTC CGCAGGTGCT TTGGAAACCC CCTTTATTGT 
GAGGTCTTAT GCCAGGATCT TCTCTCTAAA AACGTATTGT GCTTTCATGA CCTCCAAAAG 
GAGGAAGTGG AGAACAGCAA GTGGGAGACC CTCTCAGCCA ATGCCATGAA ATCCACCATG 
TACAGTGTTT CTCCTGCCAG CTCTGAAGAC CAGGAACTTT ATGTCTGCAC AGTCAAGGAT 
GATGTGAACT TGGATACAGT GCTTCTTCCA CCGTTATTAA AAGAAATAGC AGTGAACCAA 
ATAGATCAAC TGAGCCCAGA GGAACAGTCG CTGGTTAAAT GTGCTGCAGT CATTGGTCAC 
TTCTTCCACA TAGATCTGCT GCAGCACCTC CTGCCTGGCT GGAGTAAGAA TAAGCTACTT 
GAGGTACTGA AGGATCTTGT GGATATGCAC GTGCTCCACT GGTGCAATAA GGGCCAAGAG 
CTTCCTGCTG AGCCAATATT AGTGCCTTCC TCTGTCGAGA CTGAGCATAC CAAAGAGACG 
ACAAAGTCAG ATGCTGACTC TCCTCTAAAG CTACAAGAGG AACTATCCCT ACCTCAGATG 
GAGGTGCTTG AATTTGGAGT GCCTCTTTTG CGGGAAGGGG CTTGGGAGCT GTGGCCCAAG 
GCACAGCAAA CAGCCCTACA CCTTGAATGT GCCTGCTTTC TTCGAGATCT GGCCTGCCGC 
TGTGGGAGCT GTCATGGGGG AGACTTTGTC CCCTTCCACC GTTTCGCCAT CTGTTCCACT 
AAGAGCTCCA GCAGGACCTC CCGATTCTGT AGCTACAAAG ACACTGGCTC GATACTAACA 
CAAGTGATCA CAGACAGATT GCAGCTGCCT TCTCCCCAAG ACACTTTTCA GTTCCAGACA 
GAATCATCCA GAAGTCAGGA GGCCTTCACA AGGGAATACT GCAGAACAGA GGAAGAGTTC 
CTGGATAAAG TGAACAGGAA ACTGGCTCAG ACCAACCCCA AGAAAGGCAT GTTGACCACG 
AAGCCCTGCC ACTGTAGGGA AGTCCTGAAG TTAGTGCTCT CGCCCCTCAC CCAGCACTGC 
CTGGTCATTG GAGAAACCAC GTGTGCATTT TATTACCTGC TGGAATCTGC CGCTGCCTCC 
TTGGATCTGT CAGATAATTA CATGGCCTTC TTTTATTTGA GGAAGGCAAG CAGTCTTCTG 
GGCCAACCCT CCGCATTCTC GTTTTTTAAA AAGCACAAGG TGAAGATCTG TCAGTTTGAA 
GAAGCCACTT TCTGTCGTCT CCAGTCAGAG GTCTGTTTCA ACATGGGACA GATCACCCTG 
GCCAAAAAAC TGGCTAGGCG AGCCCTTCGT CTGCTGAAAA GGAGTTTTCC CTGGACCTGG 
CTTGGTGTCA TTTTCCAAAC ATTTCTAGAA AAATGCTGGT ATTCCTGTTC CCTGAGTCAA 
CCTCGAAACA ACCCTAGTGA GAAGAAGAAG AAGCTGGCAG TCCTGCAGCA GCAGATGCAC 
TGTCTCTCCC TGCTCCGGCA GCTCTACAGC CTGGAGGCCA CAGCCAGCAG CCTCAGGTTT 
GCCTGCCTGG CCACTCTTAT GCAGAAGAAT TCAGTCAAGG AGTTTCCTGG TGAAGCCCAG 
GTTGTCTCTA CCTATGTGGC CCTCTCCCAG TTCTCCCAGA ACGTGGGTGA CAAGGAGAAG 
TGGCTGTACC ATGAGCAGCT GGCCATTCAG AAAAGCAGCC TGTGCTGGTT CTCCAGGGAG 
GGACTGTTGG CCACAGCCCA GCTCATGCAG GCCTTGGCCT ACACCAAACT CTGCCTTGGG 
CATCTCGACT TCTCCATCAA GCTGGGTTTT CAAGCTCATG AGATATGCAG ACACCTCAAG 
AAACCAGCTC TGGAGAACCT GGTTCTCTCA GTTCTCTTCA GATCTGCATT TCTGAAGAAG 
AAGTTTGATC TGTGTGTCCA CGTGCTGGAG AGTCAGTGGG TGCTCATTTC CCAGAGTCAT 
GATGTCCTTG GCCGGGCCTT CTTCTATTCT GCTTGCTTAG ATCTGCTGCT CTACGGAAAA 
GGATGGCTGT GTCGGCCCTT TGGGGAATGT CTGCATTTCA TTCAACGCTA TGAGCACAGC 
TGCATTCTAA ATTCTCAGAG CAGTGTCATG CTGGGGGTCC ACTCCTCTCT GGCCATGTGG 
TTTGCCCAGG ACTTACAGTG GAATCAGTTT CAGTACCACT TCTCCAAGGC TCGCCAGTTG 
GTGAAAAGAA CCAATGCCTC ACTATTTGGT TCCCATGGCT TTGTCCGATT CCTAGAGTGC 
CAGGTGCTAA TTTTACAGAA AATGCCAGAA GATGTCTTCC TGCAAACTCT CGCGGAGACT 
CGCCGCCAAA CCCTCACGTA CTTTAAGGAG TTTTTCTCTC GATGTGTGAC CTGCCCTGTC 
TATCACCAGT GGGTCTCTGA GCTTAAAGCC TTTGTAATGA GAAATGGAAA GATGTCCTTG 
ACCAACATCA TCAGACCTTT TGACACCTGC CTGACCAGGA TTTGGATAGA GGGAGAATTC 
CTGGAGGAAA ATAACTCCTT TGAACAAAAT TTGGAAATAC AGAGATTTGT CAGAGAGGCT 
GATGTCAACA AGAAGGATAA AGAAGAAATT CAAGAATGTA TCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005192981.1
CDS	331..4938
Translation	MDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005192924.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005192924.3

ORF Insert Sequence:

ATGGACAGGT TCAGCCTGAG CTGCAAGTTG GAACATGACA TGGACAGACT GGCCCACATC 
TTCAGTTATT ACATTAGTGA CATCGTGGAG CATGTGCTCT GTTTTGGAGG AGATATCCTA 
AATATCACAG GGAATGTGCT CCTAGCACTC TGGACAGTGG AACAAAATCA ACTGAGTGAC 
ATCATTACTC TGGTGGCAAA ATGCAGTCTG GAGATACAGG AGAAATTTGG GATCTACCAT 
ACCAGAGAAG GCCAGGACCT GCAGCTAAAG ATAGGTCTAT CTGCAGGTCG GATTTCCCAG 
GTTATTGTTG GAGATGAGCA GCGCCAGTAC CTCTTGGTGA TTGGCCGGGA TGTAGATGGT 
GTGCAGTTAG CTCAACGGTT GGCTCAAGCA AATGAGATTG TCCTGTCCTG GAACTGCTGG 
AAGCTCTGTG AGCAGTACAT GTTTGAAGTG GAAATCATGC GGGAGGATGA AGCTGTGAAG 
TTAAGAGATA TGCGGATCAT TGACCCATTT GATTTTGATG AGCATTTTTA CAAGTGCCTC 
GATTATCTGC CACATTACCG GACAAGTGCT GACATTCTAA GAGCCGCCCT GGAGTTGGAT 
CCAGATTCTG CACTTGAGCA AACCCTGCGG AAGCACATAA TGAAAAACCT TTTGAAGAAG 
ATTGATGAAG GCCAGCCTAT GGAGTATATA TCCGAATTCC GCACTGTGAC TATGGTTTTG 
GTCAGCCTCG AGTTCCACAA GACAGCATGG GTGTTGCATT TGTGCCATCT TATCCAGGAG 
GCTGCGTTAT ACATCTCCAC AGTGATAGAG ACAGGGGGTG GCCAGCTGAG CCGGATCTTT 
ATGTTTGAGA CAGGCTGCAT GTTCCTCTGT GTTTTCGGCC TACCTGGGGA TAAGAAGCCA 
GACGAGTGTG CACATGCCCT GGAGAGCTCC TTCAGCATCT TCAGCTTCTG CTGGGAGAAT 
CTTGCTGAGA CCAAGCTTGT TTCCATCAGT ATCACCAATG GACCAGTATT CTGTGGCATG 
GTTGGGGCAG TTTCAAGACA CGAATATACA GTTATTGGCC CAAAAGTAAG CCTCGTGGCA 
AGAATGATAA CTGCTTATCC AGGTTTGGTG TCCTGTGATG AGGTAACATA TCTGAGATCT 
ATGCTACCTG CTTACAACTT CAAGAAACTC CCAGAGAAAA TGATGAAAAA TATCTCTAAC 
CCAGGGAAGA TGTATGAATA CGTTGGCCAT AGAAGATGTA TAATGTTTGG GAAGAGACAT 
TTGGCCACAG AGAGAAATAA AAATCACTCT TTGCTAGACC GGGAGAAAGA ACTGGAAGCT 
TTCCGAATGG CACAGCAGGA ATGTTTGCAC CAGAAGAAGG GACAGGCAGT TCTATATGAA 
GGTGGAAAAG GCAGTGGAAA AAGTCAGCTC TTGGCTGAAA TAAACTTCCT GGCCCAGAAG 
GAAGGGCACA GGGTGTTGCC GTTGAAGCTG AAGAAAGCGG ATTCCAAGCA GTGCTTCTAC 
GCCATCCAGA CCCTCATGGC CATCTTCTTC GAGATTGATA CATGCCCAGC CCATTACCGG 
CAAGAGCGCC TCCACAGACA GCTCAGTGGA ATAGTGGAGG AGCCACTTCA CTGCCTTTTT 
AATGACCTCT TCTTTGTGAA GTTTCCCTTA TCCTTCAAAG TTTCACGCAT GGATGACCTG 
GCCAGACAAC AGAAGATTAA AGCATGTTTT TTTGAAGTGC TTGAGAAGGC AGTAAGGGAA 
ACACCACTGA TTTTCCTCAT TGACGAGGCC CAGTATATGG ATGCAGCTTC CTGGGAATTT 
TTGGGAACAT TGCTCTCCGG TGTGCCTATC ATCATGGTGA TGACGTTGAC CACTACCTCC 
ACCCTGTGTG GCCAGGCCCA GCACTTCCTG CAGAGCCCTC CAGCTGTCCT GGTGCCCATT 
TCAAAGTTGG CAGCGACCTC ACTGTTGAGA ATGGCATGTT GGGAACTGGG GGTGGTGAGC 
ATCCCCCAAG AGCTGCAGAC CTACCTTCTC CGCAGGTGCT TTGGAAACCC CCTTTATTGT 
GAGGTCTTAT GCCAGGATCT TCTCTCTAAA AACGTATTGT GCTTTCATGA CCTCCAAAAG 
GAGGAAGTGG AGAACAGCAA GTGGGAGACC CTCTCAGCCA ATGCCATGAA ATCCACCATG 
TACAGTGTTT CTCCTGCCAG CTCTGAAGAC CAGGAACTTT ATGTCTGCAC AGTCAAGGAT 
GATGTGAACT TGGATACAGT GCTTCTTCCA CCGTTATTAA AAGAAATAGC AGTGAACCAA 
ATAGATCAAC TGAGCCCAGA GGAACAGTCG CTGGTTAAAT GTGCTGCAGT CATTGGTCAC 
TTCTTCCACA TAGATCTGCT GCAGCACCTC CTGCCTGGCT GGAGTAAGAA TAAGCTACTT 
GAGGTACTGA AGGATCTTGT GGATATGCAC GTGCTCCACT GGTGCAATAA GGGCCAAGAG 
CTTCCTGCTG AGCCAATATT AGTGCCTTCC TCTGTCGAGA CTGAGCATAC CAAAGAGACG 
ACAAAGTCAG ATGCTGACTC TCCTCTAAAG CTACAAGAGG AACTATCCCT ACCTCAGATG 
GAGGTGCTTG AATTTGGAGT GCCTCTTTTG CGGGAAGGGG CTTGGGAGCT GTGGCCCAAG 
GCACAGCAAA CAGCCCTACA CCTTGAATGT GCCTGCTTTC TTCGAGATCT GGCCTGCCGC 
TGTGGGAGCT GTCATGGGGG AGACTTTGTC CCCTTCCACC GTTTCGCCAT CTGTTCCACT 
AAGAGCTCCA GCAGGACCTC CCGATTCTGT AGCTACAAAG ACACTGGCTC GATACTAACA 
CAAGTGATCA CAGACAGATT GCAGCTGCCT TCTCCCCAAG ACACTTTTCA GTTCCAGACA 
GAATCATCCA GAAGTCAGGA GGCCTTCACA AGGGAATACT GCAGAACAGA GGAAGAGTTC 
CTGGATAAAG TGAACAGGAA ACTGGCTCAG ACCAACCCCA AGAAAGGCAT GTTGACCACG 
AAGCCCTGCC ACTGTAGGGA AGTCCTGAAG TTAGTGCTCT CGCCCCTCAC CCAGCACTGC 
CTGGTCATTG GAGAAACCAC GTGTGCATTT TATTACCTGC TGGAATCTGC CGCTGCCTCC 
TTGGATCTGT CAGATAATTA CATGGCCTTC TTTTATTTGA GGAAGGCAAG CAGTCTTCTG 
GGCCAACCCT CCGCATTCTC GTTTTTTAAA AAGCACAAGG TGAAGATCTG TCAGTTTGAA 
GAAGCCACTT TCTGTCGTCT CCAGTCAGAG GTCTGTTTCA ACATGGGACA GATCACCCTG 
GCCAAAAAAC TGGCTAGGCG AGCCCTTCGT CTGCTGAAAA GGAGTTTTCC CTGGACCTGG 
CTTGGTGTCA TTTTCCAAAC ATTTCTAGAA AAATGCTGGT ATTCCTGTTC CCTGAGTCAA 
CCTCGAAACA ACCCTAGTGA GAAGAAGAAG AAGCTGGCAG TCCTGCAGCA GCAGATGCAC 
TGTCTCTCCC TGCTCCGGCA GCTCTACAGC CTGGAGGCCA CAGCCAGCAG CCTCAGGTTT 
GCCTGCCTGG CCACTCTTAT GCAGAAGAAT TCAGTCAAGG AGTTTCCTGG TGAAGCCCAG 
GTTGTCTCTA CCTATGTGGC CCTCTCCCAG TTCTCCCAGA ACGTGGGTGA CAAGGAGAAG 
TGGCTGTACC ATGAGCAGCT GGCCATTCAG AAAAGCAGCC TGTGCTGGTT CTCCAGGGAG 
GGACTGTTGG CCACAGCCCA GCTCATGCAG GCCTTGGCCT ACACCAAACT CTGCCTTGGG 
CATCTCGACT TCTCCATCAA GCTGGGTTTT CAAGCTCATG AGATATGCAG ACACCTCAAG 
AAACCAGCTC TGGAGAACCT GGTTCTCTCA GTTCTCTTCA GATCTGCATT TCTGAAGAAG 
AAGTTTGATC TGTGTGTCCA CGTGCTGGAG AGTCAGTGGG TGCTCATTTC CCAGAGTCAT 
GATGTCCTTG GCCGGGCCTT CTTCTATTCT GCTTGCTTAG ATCTGCTGCT CTACGGAAAA 
GGATGGCTGT GTCGGCCCTT TGGGGAATGT CTGCATTTCA TTCAACGCTA TGAGCACAGC 
TGCATTCTAA ATTCTCAGAG CAGTGTCATG CTGGGGGTCC ACTCCTCTCT GGCCATGTGG 
TTTGCCCAGG ACTTACAGTG GAATCAGTTT CAGTACCACT TCTCCAAGGC TCGCCAGTTG 
GTGAAAAGAA CCAATGCCTC ACTATTTGGT TCCCATGGCT TTGTCCGATT CCTAGAGTGC 
CAGGTGCTAA TTTTACAGAA AATGCCAGAA GATGTCTTCC TGCAAACTCT CGCGGAGACT 
CGCCGCCAAA CCCTCACGTA CTTTAAGGAG TTTTTCTCTC GATGTGTGAC CTGCCCTGTC 
TATCACCAGT GGGTCTCTGA GCTTAAAGCC TTTGTAATGA GAAATGGAAA GATGTCCTTG 
ACCAACATCA TCAGACCTTT TGACACCTGC CTGACCAGGA TTTGGATAGA GGGAGAATTC 
CTGGAGGAAA ATAACTCCTT TGAACAAAAT TTGGAAATAC AGAGATTTGT CAGAGAGGCT 
GATGTCAACA AGAAGGATAA AGAAGAAATT CAAGAATGTA TCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005223525.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223582.1 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa11213
Clone ID Related Accession (Same CDS sequence)	XM_005192924.3 , XM_005223525.3 , XM_005223525.4
Accession Version	XM_005223525.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223525.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT TCAGCCTGAG CTGCAAGTTG GAACATGACA TGGACAGACT GGCCCACATC 
TTCAGTTATT ACATTAGTGA CATCGTGGAG CATGTGCTCT GTTTTGGAGG AGATATCCTA 
AATATCACAG GGAATGTGCT CCTAGCACTC TGGACAGTGG AACAAAATCA ACTGAGTGAC 
ATCATTACTC TGGTGGCAAA ATGCAGTCTG GAGATACAGG AGAAATTTGG GATCTACCAT 
ACCAGAGAAG GCCAGGACCT GCAGCTAAAG ATAGGTCTAT CTGCAGGTCG GATTTCCCAG 
GTTATTGTTG GAGATGAGCA GCGCCAGTAC CTCTTGGTGA TTGGCCGGGA TGTAGATGGT 
GTGCAGTTAG CTCAACGGTT GGCTCAAGCA AATGAGATTG TCCTGTCCTG GAACTGCTGG 
AAGCTCTGTG AGCAGTACAT GTTTGAAGTG GAAATCATGC GGGAGGATGA AGCTGTGAAG 
TTAAGAGATA TGCGGATCAT TGACCCATTT GATTTTGATG AGCATTTTTA CAAGTGCCTC 
GATTATCTGC CACATTACCG GACAAGTGCT GACATTCTAA GAGCCGCCCT GGAGTTGGAT 
CCAGATTCTG CACTTGAGCA AACCCTGCGG AAGCACATAA TGAAAAACCT TTTGAAGAAG 
ATTGATGAAG GCCAGCCTAT GGAGTATATA TCCGAATTCC GCACTGTGAC TATGGTTTTG 
GTCAGCCTCG AGTTCCACAA GACAGCATGG GTGTTGCATT TGTGCCATCT TATCCAGGAG 
GCTGCGTTAT ACATCTCCAC AGTGATAGAG ACAGGGGGTG GCCAGCTGAG CCGGATCTTT 
ATGTTTGAGA CAGGCTGCAT GTTCCTCTGT GTTTTCGGCC TACCTGGGGA TAAGAAGCCA 
GACGAGTGTG CACATGCCCT GGAGAGCTCC TTCAGCATCT TCAGCTTCTG CTGGGAGAAT 
CTTGCTGAGA CCAAGCTTGT TTCCATCAGT ATCACCAATG GACCAGTATT CTGTGGCATG 
GTTGGGGCAG TTTCAAGACA CGAATATACA GTTATTGGCC CAAAAGTAAG CCTCGTGGCA 
AGAATGATAA CTGCTTATCC AGGTTTGGTG TCCTGTGATG AGGTAACATA TCTGAGATCT 
ATGCTACCTG CTTACAACTT CAAGAAACTC CCAGAGAAAA TGATGAAAAA TATCTCTAAC 
CCAGGGAAGA TGTATGAATA CGTTGGCCAT AGAAGATGTA TAATGTTTGG GAAGAGACAT 
TTGGCCACAG AGAGAAATAA AAATCACTCT TTGCTAGACC GGGAGAAAGA ACTGGAAGCT 
TTCCGAATGG CACAGCAGGA ATGTTTGCAC CAGAAGAAGG GACAGGCAGT TCTATATGAA 
GGTGGAAAAG GCAGTGGAAA AAGTCAGCTC TTGGCTGAAA TAAACTTCCT GGCCCAGAAG 
GAAGGGCACA GGGTGTTGCC GTTGAAGCTG AAGAAAGCGG ATTCCAAGCA GTGCTTCTAC 
GCCATCCAGA CCCTCATGGC CATCTTCTTC GAGATTGATA CATGCCCAGC CCATTACCGG 
CAAGAGCGCC TCCACAGACA GCTCAGTGGA ATAGTGGAGG AGCCACTTCA CTGCCTTTTT 
AATGACCTCT TCTTTGTGAA GTTTCCCTTA TCCTTCAAAG TTTCACGCAT GGATGACCTG 
GCCAGACAAC AGAAGATTAA AGCATGTTTT TTTGAAGTGC TTGAGAAGGC AGTAAGGGAA 
ACACCACTGA TTTTCCTCAT TGACGAGGCC CAGTATATGG ATGCAGCTTC CTGGGAATTT 
TTGGGAACAT TGCTCTCCGG TGTGCCTATC ATCATGGTGA TGACGTTGAC CACTACCTCC 
ACCCTGTGTG GCCAGGCCCA GCACTTCCTG CAGAGCCCTC CAGCTGTCCT GGTGCCCATT 
TCAAAGTTGG CAGCGACCTC ACTGTTGAGA ATGGCATGTT GGGAACTGGG GGTGGTGAGC 
ATCCCCCAAG AGCTGCAGAC CTACCTTCTC CGCAGGTGCT TTGGAAACCC CCTTTATTGT 
GAGGTCTTAT GCCAGGATCT TCTCTCTAAA AACGTATTGT GCTTTCATGA CCTCCAAAAG 
GAGGAAGTGG AGAACAGCAA GTGGGAGACC CTCTCAGCCA ATGCCATGAA ATCCACCATG 
TACAGTGTTT CTCCTGCCAG CTCTGAAGAC CAGGAACTTT ATGTCTGCAC AGTCAAGGAT 
GATGTGAACT TGGATACAGT GCTTCTTCCA CCGTTATTAA AAGAAATAGC AGTGAACCAA 
ATAGATCAAC TGAGCCCAGA GGAACAGTCG CTGGTTAAAT GTGCTGCAGT CATTGGTCAC 
TTCTTCCACA TAGATCTGCT GCAGCACCTC CTGCCTGGCT GGAGTAAGAA TAAGCTACTT 
GAGGTACTGA AGGATCTTGT GGATATGCAC GTGCTCCACT GGTGCAATAA GGGCCAAGAG 
CTTCCTGCTG AGCCAATATT AGTGCCTTCC TCTGTCGAGA CTGAGCATAC CAAAGAGACG 
ACAAAGTCAG ATGCTGACTC TCCTCTAAAG CTACAAGAGG AACTATCCCT ACCTCAGATG 
GAGGTGCTTG AATTTGGAGT GCCTCTTTTG CGGGAAGGGG CTTGGGAGCT GTGGCCCAAG 
GCACAGCAAA CAGCCCTACA CCTTGAATGT GCCTGCTTTC TTCGAGATCT GGCCTGCCGC 
TGTGGGAGCT GTCATGGGGG AGACTTTGTC CCCTTCCACC GTTTCGCCAT CTGTTCCACT 
AAGAGCTCCA GCAGGACCTC CCGATTCTGT AGCTACAAAG ACACTGGCTC GATACTAACA 
CAAGTGATCA CAGACAGATT GCAGCTGCCT TCTCCCCAAG ACACTTTTCA GTTCCAGACA 
GAATCATCCA GAAGTCAGGA GGCCTTCACA AGGGAATACT GCAGAACAGA GGAAGAGTTC 
CTGGATAAAG TGAACAGGAA ACTGGCTCAG ACCAACCCCA AGAAAGGCAT GTTGACCACG 
AAGCCCTGCC ACTGTAGGGA AGTCCTGAAG TTAGTGCTCT CGCCCCTCAC CCAGCACTGC 
CTGGTCATTG GAGAAACCAC GTGTGCATTT TATTACCTGC TGGAATCTGC CGCTGCCTCC 
TTGGATCTGT CAGATAATTA CATGGCCTTC TTTTATTTGA GGAAGGCAAG CAGTCTTCTG 
GGCCAACCCT CCGCATTCTC GTTTTTTAAA AAGCACAAGG TGAAGATCTG TCAGTTTGAA 
GAAGCCACTT TCTGTCGTCT CCAGTCAGAG GTCTGTTTCA ACATGGGACA GATCACCCTG 
GCCAAAAAAC TGGCTAGGCG AGCCCTTCGT CTGCTGAAAA GGAGTTTTCC CTGGACCTGG 
CTTGGTGTCA TTTTCCAAAC ATTTCTAGAA AAATGCTGGT ATTCCTGTTC CCTGAGTCAA 
CCTCGAAACA ACCCTAGTGA GAAGAAGAAG AAGCTGGCAG TCCTGCAGCA GCAGATGCAC 
TGTCTCTCCC TGCTCCGGCA GCTCTACAGC CTGGAGGCCA CAGCCAGCAG CCTCAGGTTT 
GCCTGCCTGG CCACTCTTAT GCAGAAGAAT TCAGTCAAGG AGTTTCCTGG TGAAGCCCAG 
GTTGTCTCTA CCTATGTGGC CCTCTCCCAG TTCTCCCAGA ACGTGGGTGA CAAGGAGAAG 
TGGCTGTACC ATGAGCAGCT GGCCATTCAG AAAAGCAGCC TGTGCTGGTT CTCCAGGGAG 
GGACTGTTGG CCACAGCCCA GCTCATGCAG GCCTTGGCCT ACACCAAACT CTGCCTTGGG 
CATCTCGACT TCTCCATCAA GCTGGGTTTT CAAGCTCATG AGATATGCAG ACACCTCAAG 
AAACCAGCTC TGGAGAACCT GGTTCTCTCA GTTCTCTTCA GATCTGCATT TCTGAAGAAG 
AAGTTTGATC TGTGTGTCCA CGTGCTGGAG AGTCAGTGGG TGCTCATTTC CCAGAGTCAT 
GATGTCCTTG GCCGGGCCTT CTTCTATTCT GCTTGCTTAG ATCTGCTGCT CTACGGAAAA 
GGATGGCTGT GTCGGCCCTT TGGGGAATGT CTGCATTTCA TTCAACGCTA TGAGCACAGC 
TGCATTCTAA ATTCTCAGAG CAGTGTCATG CTGGGGGTCC ACTCCTCTCT GGCCATGTGG 
TTTGCCCAGG ACTTACAGTG GAATCAGTTT CAGTACCACT TCTCCAAGGC TCGCCAGTTG 
GTGAAAAGAA CCAATGCCTC ACTATTTGGT TCCCATGGCT TTGTCCGATT CCTAGAGTGC 
CAGGTGCTAA TTTTACAGAA AATGCCAGAA GATGTCTTCC TGCAAACTCT CGCGGAGACT 
CGCCGCCAAA CCCTCACGTA CTTTAAGGAG TTTTTCTCTC GATGTGTGAC CTGCCCTGTC 
TATCACCAGT GGGTCTCTGA GCTTAAAGCC TTTGTAATGA GAAATGGAAA GATGTCCTTG 
ACCAACATCA TCAGACCTTT TGACACCTGC CTGACCAGGA TTTGGATAGA GGGAGAATTC 
CTGGAGGAAA ATAACTCCTT TGAACAAAAT TTGGAAATAC AGAGATTTGT CAGAGAGGCT 
GATGTCAACA AGAAGGATAA AGAAGAAATT CAAGAATGTA TCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223582.1
CDS	331..4938
Translation	MDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005223525.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223525.3

ORF Insert Sequence:

ATGGACAGGT TCAGCCTGAG CTGCAAGTTG GAACATGACA TGGACAGACT GGCCCACATC 
TTCAGTTATT ACATTAGTGA CATCGTGGAG CATGTGCTCT GTTTTGGAGG AGATATCCTA 
AATATCACAG GGAATGTGCT CCTAGCACTC TGGACAGTGG AACAAAATCA ACTGAGTGAC 
ATCATTACTC TGGTGGCAAA ATGCAGTCTG GAGATACAGG AGAAATTTGG GATCTACCAT 
ACCAGAGAAG GCCAGGACCT GCAGCTAAAG ATAGGTCTAT CTGCAGGTCG GATTTCCCAG 
GTTATTGTTG GAGATGAGCA GCGCCAGTAC CTCTTGGTGA TTGGCCGGGA TGTAGATGGT 
GTGCAGTTAG CTCAACGGTT GGCTCAAGCA AATGAGATTG TCCTGTCCTG GAACTGCTGG 
AAGCTCTGTG AGCAGTACAT GTTTGAAGTG GAAATCATGC GGGAGGATGA AGCTGTGAAG 
TTAAGAGATA TGCGGATCAT TGACCCATTT GATTTTGATG AGCATTTTTA CAAGTGCCTC 
GATTATCTGC CACATTACCG GACAAGTGCT GACATTCTAA GAGCCGCCCT GGAGTTGGAT 
CCAGATTCTG CACTTGAGCA AACCCTGCGG AAGCACATAA TGAAAAACCT TTTGAAGAAG 
ATTGATGAAG GCCAGCCTAT GGAGTATATA TCCGAATTCC GCACTGTGAC TATGGTTTTG 
GTCAGCCTCG AGTTCCACAA GACAGCATGG GTGTTGCATT TGTGCCATCT TATCCAGGAG 
GCTGCGTTAT ACATCTCCAC AGTGATAGAG ACAGGGGGTG GCCAGCTGAG CCGGATCTTT 
ATGTTTGAGA CAGGCTGCAT GTTCCTCTGT GTTTTCGGCC TACCTGGGGA TAAGAAGCCA 
GACGAGTGTG CACATGCCCT GGAGAGCTCC TTCAGCATCT TCAGCTTCTG CTGGGAGAAT 
CTTGCTGAGA CCAAGCTTGT TTCCATCAGT ATCACCAATG GACCAGTATT CTGTGGCATG 
GTTGGGGCAG TTTCAAGACA CGAATATACA GTTATTGGCC CAAAAGTAAG CCTCGTGGCA 
AGAATGATAA CTGCTTATCC AGGTTTGGTG TCCTGTGATG AGGTAACATA TCTGAGATCT 
ATGCTACCTG CTTACAACTT CAAGAAACTC CCAGAGAAAA TGATGAAAAA TATCTCTAAC 
CCAGGGAAGA TGTATGAATA CGTTGGCCAT AGAAGATGTA TAATGTTTGG GAAGAGACAT 
TTGGCCACAG AGAGAAATAA AAATCACTCT TTGCTAGACC GGGAGAAAGA ACTGGAAGCT 
TTCCGAATGG CACAGCAGGA ATGTTTGCAC CAGAAGAAGG GACAGGCAGT TCTATATGAA 
GGTGGAAAAG GCAGTGGAAA AAGTCAGCTC TTGGCTGAAA TAAACTTCCT GGCCCAGAAG 
GAAGGGCACA GGGTGTTGCC GTTGAAGCTG AAGAAAGCGG ATTCCAAGCA GTGCTTCTAC 
GCCATCCAGA CCCTCATGGC CATCTTCTTC GAGATTGATA CATGCCCAGC CCATTACCGG 
CAAGAGCGCC TCCACAGACA GCTCAGTGGA ATAGTGGAGG AGCCACTTCA CTGCCTTTTT 
AATGACCTCT TCTTTGTGAA GTTTCCCTTA TCCTTCAAAG TTTCACGCAT GGATGACCTG 
GCCAGACAAC AGAAGATTAA AGCATGTTTT TTTGAAGTGC TTGAGAAGGC AGTAAGGGAA 
ACACCACTGA TTTTCCTCAT TGACGAGGCC CAGTATATGG ATGCAGCTTC CTGGGAATTT 
TTGGGAACAT TGCTCTCCGG TGTGCCTATC ATCATGGTGA TGACGTTGAC CACTACCTCC 
ACCCTGTGTG GCCAGGCCCA GCACTTCCTG CAGAGCCCTC CAGCTGTCCT GGTGCCCATT 
TCAAAGTTGG CAGCGACCTC ACTGTTGAGA ATGGCATGTT GGGAACTGGG GGTGGTGAGC 
ATCCCCCAAG AGCTGCAGAC CTACCTTCTC CGCAGGTGCT TTGGAAACCC CCTTTATTGT 
GAGGTCTTAT GCCAGGATCT TCTCTCTAAA AACGTATTGT GCTTTCATGA CCTCCAAAAG 
GAGGAAGTGG AGAACAGCAA GTGGGAGACC CTCTCAGCCA ATGCCATGAA ATCCACCATG 
TACAGTGTTT CTCCTGCCAG CTCTGAAGAC CAGGAACTTT ATGTCTGCAC AGTCAAGGAT 
GATGTGAACT TGGATACAGT GCTTCTTCCA CCGTTATTAA AAGAAATAGC AGTGAACCAA 
ATAGATCAAC TGAGCCCAGA GGAACAGTCG CTGGTTAAAT GTGCTGCAGT CATTGGTCAC 
TTCTTCCACA TAGATCTGCT GCAGCACCTC CTGCCTGGCT GGAGTAAGAA TAAGCTACTT 
GAGGTACTGA AGGATCTTGT GGATATGCAC GTGCTCCACT GGTGCAATAA GGGCCAAGAG 
CTTCCTGCTG AGCCAATATT AGTGCCTTCC TCTGTCGAGA CTGAGCATAC CAAAGAGACG 
ACAAAGTCAG ATGCTGACTC TCCTCTAAAG CTACAAGAGG AACTATCCCT ACCTCAGATG 
GAGGTGCTTG AATTTGGAGT GCCTCTTTTG CGGGAAGGGG CTTGGGAGCT GTGGCCCAAG 
GCACAGCAAA CAGCCCTACA CCTTGAATGT GCCTGCTTTC TTCGAGATCT GGCCTGCCGC 
TGTGGGAGCT GTCATGGGGG AGACTTTGTC CCCTTCCACC GTTTCGCCAT CTGTTCCACT 
AAGAGCTCCA GCAGGACCTC CCGATTCTGT AGCTACAAAG ACACTGGCTC GATACTAACA 
CAAGTGATCA CAGACAGATT GCAGCTGCCT TCTCCCCAAG ACACTTTTCA GTTCCAGACA 
GAATCATCCA GAAGTCAGGA GGCCTTCACA AGGGAATACT GCAGAACAGA GGAAGAGTTC 
CTGGATAAAG TGAACAGGAA ACTGGCTCAG ACCAACCCCA AGAAAGGCAT GTTGACCACG 
AAGCCCTGCC ACTGTAGGGA AGTCCTGAAG TTAGTGCTCT CGCCCCTCAC CCAGCACTGC 
CTGGTCATTG GAGAAACCAC GTGTGCATTT TATTACCTGC TGGAATCTGC CGCTGCCTCC 
TTGGATCTGT CAGATAATTA CATGGCCTTC TTTTATTTGA GGAAGGCAAG CAGTCTTCTG 
GGCCAACCCT CCGCATTCTC GTTTTTTAAA AAGCACAAGG TGAAGATCTG TCAGTTTGAA 
GAAGCCACTT TCTGTCGTCT CCAGTCAGAG GTCTGTTTCA ACATGGGACA GATCACCCTG 
GCCAAAAAAC TGGCTAGGCG AGCCCTTCGT CTGCTGAAAA GGAGTTTTCC CTGGACCTGG 
CTTGGTGTCA TTTTCCAAAC ATTTCTAGAA AAATGCTGGT ATTCCTGTTC CCTGAGTCAA 
CCTCGAAACA ACCCTAGTGA GAAGAAGAAG AAGCTGGCAG TCCTGCAGCA GCAGATGCAC 
TGTCTCTCCC TGCTCCGGCA GCTCTACAGC CTGGAGGCCA CAGCCAGCAG CCTCAGGTTT 
GCCTGCCTGG CCACTCTTAT GCAGAAGAAT TCAGTCAAGG AGTTTCCTGG TGAAGCCCAG 
GTTGTCTCTA CCTATGTGGC CCTCTCCCAG TTCTCCCAGA ACGTGGGTGA CAAGGAGAAG 
TGGCTGTACC ATGAGCAGCT GGCCATTCAG AAAAGCAGCC TGTGCTGGTT CTCCAGGGAG 
GGACTGTTGG CCACAGCCCA GCTCATGCAG GCCTTGGCCT ACACCAAACT CTGCCTTGGG 
CATCTCGACT TCTCCATCAA GCTGGGTTTT CAAGCTCATG AGATATGCAG ACACCTCAAG 
AAACCAGCTC TGGAGAACCT GGTTCTCTCA GTTCTCTTCA GATCTGCATT TCTGAAGAAG 
AAGTTTGATC TGTGTGTCCA CGTGCTGGAG AGTCAGTGGG TGCTCATTTC CCAGAGTCAT 
GATGTCCTTG GCCGGGCCTT CTTCTATTCT GCTTGCTTAG ATCTGCTGCT CTACGGAAAA 
GGATGGCTGT GTCGGCCCTT TGGGGAATGT CTGCATTTCA TTCAACGCTA TGAGCACAGC 
TGCATTCTAA ATTCTCAGAG CAGTGTCATG CTGGGGGTCC ACTCCTCTCT GGCCATGTGG 
TTTGCCCAGG ACTTACAGTG GAATCAGTTT CAGTACCACT TCTCCAAGGC TCGCCAGTTG 
GTGAAAAGAA CCAATGCCTC ACTATTTGGT TCCCATGGCT TTGTCCGATT CCTAGAGTGC 
CAGGTGCTAA TTTTACAGAA AATGCCAGAA GATGTCTTCC TGCAAACTCT CGCGGAGACT 
CGCCGCCAAA CCCTCACGTA CTTTAAGGAG TTTTTCTCTC GATGTGTGAC CTGCCCTGTC 
TATCACCAGT GGGTCTCTGA GCTTAAAGCC TTTGTAATGA GAAATGGAAA GATGTCCTTG 
ACCAACATCA TCAGACCTTT TGACACCTGC CTGACCAGGA TTTGGATAGA GGGAGAATTC 
CTGGAGGAAA ATAACTCCTT TGAACAAAAT TTGGAAATAC AGAGATTTGT CAGAGAGGCT 
GATGTCAACA AGAAGGATAA AGAAGAAATT CAAGAATGTA TCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005223525.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223582.1 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa11213
Clone ID Related Accession (Same CDS sequence)	XM_005192924.3 , XM_005223525.3 , XM_005223525.4
Accession Version	XM_005223525.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223525.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT TCAGCCTGAG CTGCAAGTTG GAACATGACA TGGACAGACT GGCCCACATC 
TTCAGTTATT ACATTAGTGA CATCGTGGAG CATGTGCTCT GTTTTGGAGG AGATATCCTA 
AATATCACAG GGAATGTGCT CCTAGCACTC TGGACAGTGG AACAAAATCA ACTGAGTGAC 
ATCATTACTC TGGTGGCAAA ATGCAGTCTG GAGATACAGG AGAAATTTGG GATCTACCAT 
ACCAGAGAAG GCCAGGACCT GCAGCTAAAG ATAGGTCTAT CTGCAGGTCG GATTTCCCAG 
GTTATTGTTG GAGATGAGCA GCGCCAGTAC CTCTTGGTGA TTGGCCGGGA TGTAGATGGT 
GTGCAGTTAG CTCAACGGTT GGCTCAAGCA AATGAGATTG TCCTGTCCTG GAACTGCTGG 
AAGCTCTGTG AGCAGTACAT GTTTGAAGTG GAAATCATGC GGGAGGATGA AGCTGTGAAG 
TTAAGAGATA TGCGGATCAT TGACCCATTT GATTTTGATG AGCATTTTTA CAAGTGCCTC 
GATTATCTGC CACATTACCG GACAAGTGCT GACATTCTAA GAGCCGCCCT GGAGTTGGAT 
CCAGATTCTG CACTTGAGCA AACCCTGCGG AAGCACATAA TGAAAAACCT TTTGAAGAAG 
ATTGATGAAG GCCAGCCTAT GGAGTATATA TCCGAATTCC GCACTGTGAC TATGGTTTTG 
GTCAGCCTCG AGTTCCACAA GACAGCATGG GTGTTGCATT TGTGCCATCT TATCCAGGAG 
GCTGCGTTAT ACATCTCCAC AGTGATAGAG ACAGGGGGTG GCCAGCTGAG CCGGATCTTT 
ATGTTTGAGA CAGGCTGCAT GTTCCTCTGT GTTTTCGGCC TACCTGGGGA TAAGAAGCCA 
GACGAGTGTG CACATGCCCT GGAGAGCTCC TTCAGCATCT TCAGCTTCTG CTGGGAGAAT 
CTTGCTGAGA CCAAGCTTGT TTCCATCAGT ATCACCAATG GACCAGTATT CTGTGGCATG 
GTTGGGGCAG TTTCAAGACA CGAATATACA GTTATTGGCC CAAAAGTAAG CCTCGTGGCA 
AGAATGATAA CTGCTTATCC AGGTTTGGTG TCCTGTGATG AGGTAACATA TCTGAGATCT 
ATGCTACCTG CTTACAACTT CAAGAAACTC CCAGAGAAAA TGATGAAAAA TATCTCTAAC 
CCAGGGAAGA TGTATGAATA CGTTGGCCAT AGAAGATGTA TAATGTTTGG GAAGAGACAT 
TTGGCCACAG AGAGAAATAA AAATCACTCT TTGCTAGACC GGGAGAAAGA ACTGGAAGCT 
TTCCGAATGG CACAGCAGGA ATGTTTGCAC CAGAAGAAGG GACAGGCAGT TCTATATGAA 
GGTGGAAAAG GCAGTGGAAA AAGTCAGCTC TTGGCTGAAA TAAACTTCCT GGCCCAGAAG 
GAAGGGCACA GGGTGTTGCC GTTGAAGCTG AAGAAAGCGG ATTCCAAGCA GTGCTTCTAC 
GCCATCCAGA CCCTCATGGC CATCTTCTTC GAGATTGATA CATGCCCAGC CCATTACCGG 
CAAGAGCGCC TCCACAGACA GCTCAGTGGA ATAGTGGAGG AGCCACTTCA CTGCCTTTTT 
AATGACCTCT TCTTTGTGAA GTTTCCCTTA TCCTTCAAAG TTTCACGCAT GGATGACCTG 
GCCAGACAAC AGAAGATTAA AGCATGTTTT TTTGAAGTGC TTGAGAAGGC AGTAAGGGAA 
ACACCACTGA TTTTCCTCAT TGACGAGGCC CAGTATATGG ATGCAGCTTC CTGGGAATTT 
TTGGGAACAT TGCTCTCCGG TGTGCCTATC ATCATGGTGA TGACGTTGAC CACTACCTCC 
ACCCTGTGTG GCCAGGCCCA GCACTTCCTG CAGAGCCCTC CAGCTGTCCT GGTGCCCATT 
TCAAAGTTGG CAGCGACCTC ACTGTTGAGA ATGGCATGTT GGGAACTGGG GGTGGTGAGC 
ATCCCCCAAG AGCTGCAGAC CTACCTTCTC CGCAGGTGCT TTGGAAACCC CCTTTATTGT 
GAGGTCTTAT GCCAGGATCT TCTCTCTAAA AACGTATTGT GCTTTCATGA CCTCCAAAAG 
GAGGAAGTGG AGAACAGCAA GTGGGAGACC CTCTCAGCCA ATGCCATGAA ATCCACCATG 
TACAGTGTTT CTCCTGCCAG CTCTGAAGAC CAGGAACTTT ATGTCTGCAC AGTCAAGGAT 
GATGTGAACT TGGATACAGT GCTTCTTCCA CCGTTATTAA AAGAAATAGC AGTGAACCAA 
ATAGATCAAC TGAGCCCAGA GGAACAGTCG CTGGTTAAAT GTGCTGCAGT CATTGGTCAC 
TTCTTCCACA TAGATCTGCT GCAGCACCTC CTGCCTGGCT GGAGTAAGAA TAAGCTACTT 
GAGGTACTGA AGGATCTTGT GGATATGCAC GTGCTCCACT GGTGCAATAA GGGCCAAGAG 
CTTCCTGCTG AGCCAATATT AGTGCCTTCC TCTGTCGAGA CTGAGCATAC CAAAGAGACG 
ACAAAGTCAG ATGCTGACTC TCCTCTAAAG CTACAAGAGG AACTATCCCT ACCTCAGATG 
GAGGTGCTTG AATTTGGAGT GCCTCTTTTG CGGGAAGGGG CTTGGGAGCT GTGGCCCAAG 
GCACAGCAAA CAGCCCTACA CCTTGAATGT GCCTGCTTTC TTCGAGATCT GGCCTGCCGC 
TGTGGGAGCT GTCATGGGGG AGACTTTGTC CCCTTCCACC GTTTCGCCAT CTGTTCCACT 
AAGAGCTCCA GCAGGACCTC CCGATTCTGT AGCTACAAAG ACACTGGCTC GATACTAACA 
CAAGTGATCA CAGACAGATT GCAGCTGCCT TCTCCCCAAG ACACTTTTCA GTTCCAGACA 
GAATCATCCA GAAGTCAGGA GGCCTTCACA AGGGAATACT GCAGAACAGA GGAAGAGTTC 
CTGGATAAAG TGAACAGGAA ACTGGCTCAG ACCAACCCCA AGAAAGGCAT GTTGACCACG 
AAGCCCTGCC ACTGTAGGGA AGTCCTGAAG TTAGTGCTCT CGCCCCTCAC CCAGCACTGC 
CTGGTCATTG GAGAAACCAC GTGTGCATTT TATTACCTGC TGGAATCTGC CGCTGCCTCC 
TTGGATCTGT CAGATAATTA CATGGCCTTC TTTTATTTGA GGAAGGCAAG CAGTCTTCTG 
GGCCAACCCT CCGCATTCTC GTTTTTTAAA AAGCACAAGG TGAAGATCTG TCAGTTTGAA 
GAAGCCACTT TCTGTCGTCT CCAGTCAGAG GTCTGTTTCA ACATGGGACA GATCACCCTG 
GCCAAAAAAC TGGCTAGGCG AGCCCTTCGT CTGCTGAAAA GGAGTTTTCC CTGGACCTGG 
CTTGGTGTCA TTTTCCAAAC ATTTCTAGAA AAATGCTGGT ATTCCTGTTC CCTGAGTCAA 
CCTCGAAACA ACCCTAGTGA GAAGAAGAAG AAGCTGGCAG TCCTGCAGCA GCAGATGCAC 
TGTCTCTCCC TGCTCCGGCA GCTCTACAGC CTGGAGGCCA CAGCCAGCAG CCTCAGGTTT 
GCCTGCCTGG CCACTCTTAT GCAGAAGAAT TCAGTCAAGG AGTTTCCTGG TGAAGCCCAG 
GTTGTCTCTA CCTATGTGGC CCTCTCCCAG TTCTCCCAGA ACGTGGGTGA CAAGGAGAAG 
TGGCTGTACC ATGAGCAGCT GGCCATTCAG AAAAGCAGCC TGTGCTGGTT CTCCAGGGAG 
GGACTGTTGG CCACAGCCCA GCTCATGCAG GCCTTGGCCT ACACCAAACT CTGCCTTGGG 
CATCTCGACT TCTCCATCAA GCTGGGTTTT CAAGCTCATG AGATATGCAG ACACCTCAAG 
AAACCAGCTC TGGAGAACCT GGTTCTCTCA GTTCTCTTCA GATCTGCATT TCTGAAGAAG 
AAGTTTGATC TGTGTGTCCA CGTGCTGGAG AGTCAGTGGG TGCTCATTTC CCAGAGTCAT 
GATGTCCTTG GCCGGGCCTT CTTCTATTCT GCTTGCTTAG ATCTGCTGCT CTACGGAAAA 
GGATGGCTGT GTCGGCCCTT TGGGGAATGT CTGCATTTCA TTCAACGCTA TGAGCACAGC 
TGCATTCTAA ATTCTCAGAG CAGTGTCATG CTGGGGGTCC ACTCCTCTCT GGCCATGTGG 
TTTGCCCAGG ACTTACAGTG GAATCAGTTT CAGTACCACT TCTCCAAGGC TCGCCAGTTG 
GTGAAAAGAA CCAATGCCTC ACTATTTGGT TCCCATGGCT TTGTCCGATT CCTAGAGTGC 
CAGGTGCTAA TTTTACAGAA AATGCCAGAA GATGTCTTCC TGCAAACTCT CGCGGAGACT 
CGCCGCCAAA CCCTCACGTA CTTTAAGGAG TTTTTCTCTC GATGTGTGAC CTGCCCTGTC 
TATCACCAGT GGGTCTCTGA GCTTAAAGCC TTTGTAATGA GAAATGGAAA GATGTCCTTG 
ACCAACATCA TCAGACCTTT TGACACCTGC CTGACCAGGA TTTGGATAGA GGGAGAATTC 
CTGGAGGAAA ATAACTCCTT TGAACAAAAT TTGGAAATAC AGAGATTTGT CAGAGAGGCT 
GATGTCAACA AGAAGGATAA AGAAGAAATT CAAGAATGTA TCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223582.1
CDS	336..4943
Translation	MDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005223525.4
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223525.4

ORF Insert Sequence:

ATGGACAGGT TCAGCCTGAG CTGCAAGTTG GAACATGACA TGGACAGACT GGCCCACATC 
TTCAGTTATT ACATTAGTGA CATCGTGGAG CATGTGCTCT GTTTTGGAGG AGATATCCTA 
AATATCACAG GGAATGTGCT CCTAGCACTC TGGACAGTGG AACAAAATCA ACTGAGTGAC 
ATCATTACTC TGGTGGCAAA ATGCAGTCTG GAGATACAGG AGAAATTTGG GATCTACCAT 
ACCAGAGAAG GCCAGGACCT GCAGCTAAAG ATAGGTCTAT CTGCAGGTCG GATTTCCCAG 
GTTATTGTTG GAGATGAGCA GCGCCAGTAC CTCTTGGTGA TTGGCCGGGA TGTAGATGGT 
GTGCAGTTAG CTCAACGGTT GGCTCAAGCA AATGAGATTG TCCTGTCCTG GAACTGCTGG 
AAGCTCTGTG AGCAGTACAT GTTTGAAGTG GAAATCATGC GGGAGGATGA AGCTGTGAAG 
TTAAGAGATA TGCGGATCAT TGACCCATTT GATTTTGATG AGCATTTTTA CAAGTGCCTC 
GATTATCTGC CACATTACCG GACAAGTGCT GACATTCTAA GAGCCGCCCT GGAGTTGGAT 
CCAGATTCTG CACTTGAGCA AACCCTGCGG AAGCACATAA TGAAAAACCT TTTGAAGAAG 
ATTGATGAAG GCCAGCCTAT GGAGTATATA TCCGAATTCC GCACTGTGAC TATGGTTTTG 
GTCAGCCTCG AGTTCCACAA GACAGCATGG GTGTTGCATT TGTGCCATCT TATCCAGGAG 
GCTGCGTTAT ACATCTCCAC AGTGATAGAG ACAGGGGGTG GCCAGCTGAG CCGGATCTTT 
ATGTTTGAGA CAGGCTGCAT GTTCCTCTGT GTTTTCGGCC TACCTGGGGA TAAGAAGCCA 
GACGAGTGTG CACATGCCCT GGAGAGCTCC TTCAGCATCT TCAGCTTCTG CTGGGAGAAT 
CTTGCTGAGA CCAAGCTTGT TTCCATCAGT ATCACCAATG GACCAGTATT CTGTGGCATG 
GTTGGGGCAG TTTCAAGACA CGAATATACA GTTATTGGCC CAAAAGTAAG CCTCGTGGCA 
AGAATGATAA CTGCTTATCC AGGTTTGGTG TCCTGTGATG AGGTAACATA TCTGAGATCT 
ATGCTACCTG CTTACAACTT CAAGAAACTC CCAGAGAAAA TGATGAAAAA TATCTCTAAC 
CCAGGGAAGA TGTATGAATA CGTTGGCCAT AGAAGATGTA TAATGTTTGG GAAGAGACAT 
TTGGCCACAG AGAGAAATAA AAATCACTCT TTGCTAGACC GGGAGAAAGA ACTGGAAGCT 
TTCCGAATGG CACAGCAGGA ATGTTTGCAC CAGAAGAAGG GACAGGCAGT TCTATATGAA 
GGTGGAAAAG GCAGTGGAAA AAGTCAGCTC TTGGCTGAAA TAAACTTCCT GGCCCAGAAG 
GAAGGGCACA GGGTGTTGCC GTTGAAGCTG AAGAAAGCGG ATTCCAAGCA GTGCTTCTAC 
GCCATCCAGA CCCTCATGGC CATCTTCTTC GAGATTGATA CATGCCCAGC CCATTACCGG 
CAAGAGCGCC TCCACAGACA GCTCAGTGGA ATAGTGGAGG AGCCACTTCA CTGCCTTTTT 
AATGACCTCT TCTTTGTGAA GTTTCCCTTA TCCTTCAAAG TTTCACGCAT GGATGACCTG 
GCCAGACAAC AGAAGATTAA AGCATGTTTT TTTGAAGTGC TTGAGAAGGC AGTAAGGGAA 
ACACCACTGA TTTTCCTCAT TGACGAGGCC CAGTATATGG ATGCAGCTTC CTGGGAATTT 
TTGGGAACAT TGCTCTCCGG TGTGCCTATC ATCATGGTGA TGACGTTGAC CACTACCTCC 
ACCCTGTGTG GCCAGGCCCA GCACTTCCTG CAGAGCCCTC CAGCTGTCCT GGTGCCCATT 
TCAAAGTTGG CAGCGACCTC ACTGTTGAGA ATGGCATGTT GGGAACTGGG GGTGGTGAGC 
ATCCCCCAAG AGCTGCAGAC CTACCTTCTC CGCAGGTGCT TTGGAAACCC CCTTTATTGT 
GAGGTCTTAT GCCAGGATCT TCTCTCTAAA AACGTATTGT GCTTTCATGA CCTCCAAAAG 
GAGGAAGTGG AGAACAGCAA GTGGGAGACC CTCTCAGCCA ATGCCATGAA ATCCACCATG 
TACAGTGTTT CTCCTGCCAG CTCTGAAGAC CAGGAACTTT ATGTCTGCAC AGTCAAGGAT 
GATGTGAACT TGGATACAGT GCTTCTTCCA CCGTTATTAA AAGAAATAGC AGTGAACCAA 
ATAGATCAAC TGAGCCCAGA GGAACAGTCG CTGGTTAAAT GTGCTGCAGT CATTGGTCAC 
TTCTTCCACA TAGATCTGCT GCAGCACCTC CTGCCTGGCT GGAGTAAGAA TAAGCTACTT 
GAGGTACTGA AGGATCTTGT GGATATGCAC GTGCTCCACT GGTGCAATAA GGGCCAAGAG 
CTTCCTGCTG AGCCAATATT AGTGCCTTCC TCTGTCGAGA CTGAGCATAC CAAAGAGACG 
ACAAAGTCAG ATGCTGACTC TCCTCTAAAG CTACAAGAGG AACTATCCCT ACCTCAGATG 
GAGGTGCTTG AATTTGGAGT GCCTCTTTTG CGGGAAGGGG CTTGGGAGCT GTGGCCCAAG 
GCACAGCAAA CAGCCCTACA CCTTGAATGT GCCTGCTTTC TTCGAGATCT GGCCTGCCGC 
TGTGGGAGCT GTCATGGGGG AGACTTTGTC CCCTTCCACC GTTTCGCCAT CTGTTCCACT 
AAGAGCTCCA GCAGGACCTC CCGATTCTGT AGCTACAAAG ACACTGGCTC GATACTAACA 
CAAGTGATCA CAGACAGATT GCAGCTGCCT TCTCCCCAAG ACACTTTTCA GTTCCAGACA 
GAATCATCCA GAAGTCAGGA GGCCTTCACA AGGGAATACT GCAGAACAGA GGAAGAGTTC 
CTGGATAAAG TGAACAGGAA ACTGGCTCAG ACCAACCCCA AGAAAGGCAT GTTGACCACG 
AAGCCCTGCC ACTGTAGGGA AGTCCTGAAG TTAGTGCTCT CGCCCCTCAC CCAGCACTGC 
CTGGTCATTG GAGAAACCAC GTGTGCATTT TATTACCTGC TGGAATCTGC CGCTGCCTCC 
TTGGATCTGT CAGATAATTA CATGGCCTTC TTTTATTTGA GGAAGGCAAG CAGTCTTCTG 
GGCCAACCCT CCGCATTCTC GTTTTTTAAA AAGCACAAGG TGAAGATCTG TCAGTTTGAA 
GAAGCCACTT TCTGTCGTCT CCAGTCAGAG GTCTGTTTCA ACATGGGACA GATCACCCTG 
GCCAAAAAAC TGGCTAGGCG AGCCCTTCGT CTGCTGAAAA GGAGTTTTCC CTGGACCTGG 
CTTGGTGTCA TTTTCCAAAC ATTTCTAGAA AAATGCTGGT ATTCCTGTTC CCTGAGTCAA 
CCTCGAAACA ACCCTAGTGA GAAGAAGAAG AAGCTGGCAG TCCTGCAGCA GCAGATGCAC 
TGTCTCTCCC TGCTCCGGCA GCTCTACAGC CTGGAGGCCA CAGCCAGCAG CCTCAGGTTT 
GCCTGCCTGG CCACTCTTAT GCAGAAGAAT TCAGTCAAGG AGTTTCCTGG TGAAGCCCAG 
GTTGTCTCTA CCTATGTGGC CCTCTCCCAG TTCTCCCAGA ACGTGGGTGA CAAGGAGAAG 
TGGCTGTACC ATGAGCAGCT GGCCATTCAG AAAAGCAGCC TGTGCTGGTT CTCCAGGGAG 
GGACTGTTGG CCACAGCCCA GCTCATGCAG GCCTTGGCCT ACACCAAACT CTGCCTTGGG 
CATCTCGACT TCTCCATCAA GCTGGGTTTT CAAGCTCATG AGATATGCAG ACACCTCAAG 
AAACCAGCTC TGGAGAACCT GGTTCTCTCA GTTCTCTTCA GATCTGCATT TCTGAAGAAG 
AAGTTTGATC TGTGTGTCCA CGTGCTGGAG AGTCAGTGGG TGCTCATTTC CCAGAGTCAT 
GATGTCCTTG GCCGGGCCTT CTTCTATTCT GCTTGCTTAG ATCTGCTGCT CTACGGAAAA 
GGATGGCTGT GTCGGCCCTT TGGGGAATGT CTGCATTTCA TTCAACGCTA TGAGCACAGC 
TGCATTCTAA ATTCTCAGAG CAGTGTCATG CTGGGGGTCC ACTCCTCTCT GGCCATGTGG 
TTTGCCCAGG ACTTACAGTG GAATCAGTTT CAGTACCACT TCTCCAAGGC TCGCCAGTTG 
GTGAAAAGAA CCAATGCCTC ACTATTTGGT TCCCATGGCT TTGTCCGATT CCTAGAGTGC 
CAGGTGCTAA TTTTACAGAA AATGCCAGAA GATGTCTTCC TGCAAACTCT CGCGGAGACT 
CGCCGCCAAA CCCTCACGTA CTTTAAGGAG TTTTTCTCTC GATGTGTGAC CTGCCCTGTC 
TATCACCAGT GGGTCTCTGA GCTTAAAGCC TTTGTAATGA GAAATGGAAA GATGTCCTTG 
ACCAACATCA TCAGACCTTT TGACACCTGC CTGACCAGGA TTTGGATAGA GGGAGAATTC 
CTGGAGGAAA ATAACTCCTT TGAACAAAAT TTGGAAATAC AGAGATTTGT CAGAGAGGCT 
GATGTCAACA AGAAGGATAA AGAAGAAATT CAAGAATGTA TCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005223521.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223578.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.

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CloneID	OBa52049
Clone ID Related Accession (Same CDS sequence)	XM_005223521.3 , XM_005192920.3 , XM_005223521.4
Accession Version	XM_005223521.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTTT 
GCCCAGGACT TACAGTGGAA TCAGTTTCAG TACCACTTCT CCAAGGCTCG CCAGTTGGTG 
AAAAGAACCA ATGCCTCACT ATTTGGTTCC CATGGCTTTG TCCGATTCCT AGAGTGCCAG 
GTGCTAATTT TACAGAAAAT GCCAGAAGAT GTCTTCCTGC AAACTCTCGC GGAGACTCGC 
CGCCAAACCC TCACGTACTT TAAGGAGTTT TTCTCTCGAT GTGTGACCTG CCCTGTCTAT 
CACCAGTGGG TCTCTGAGCT TAAAGCCTTT GTAATGAGAA ATGGAAAGAT GTCCTTGACC 
AACATCATCA GACCTTTTGA CACCTGCCTG ACCAGGATTT GGATAGAGGG AGAATTCCTG 
GAGGAAAATA ACTCCTTTGA ACAAAATTTG GAAATACAGA GATTTGTCAG AGAGGCTGAT 
GTCAACAAGA AGGATAAAGA AGAAATTCAA GAATGTATCT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223578.2
CDS	16..4920
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005223521.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223521.3

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTTT 
GCCCAGGACT TACAGTGGAA TCAGTTTCAG TACCACTTCT CCAAGGCTCG CCAGTTGGTG 
AAAAGAACCA ATGCCTCACT ATTTGGTTCC CATGGCTTTG TCCGATTCCT AGAGTGCCAG 
GTGCTAATTT TACAGAAAAT GCCAGAAGAT GTCTTCCTGC AAACTCTCGC GGAGACTCGC 
CGCCAAACCC TCACGTACTT TAAGGAGTTT TTCTCTCGAT GTGTGACCTG CCCTGTCTAT 
CACCAGTGGG TCTCTGAGCT TAAAGCCTTT GTAATGAGAA ATGGAAAGAT GTCCTTGACC 
AACATCATCA GACCTTTTGA CACCTGCCTG ACCAGGATTT GGATAGAGGG AGAATTCCTG 
GAGGAAAATA ACTCCTTTGA ACAAAATTTG GAAATACAGA GATTTGTCAG AGAGGCTGAT 
GTCAACAAGA AGGATAAAGA AGAAATTCAA GAATGTATCT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005192920.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005192977.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.

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CloneID	OBa52049
Clone ID Related Accession (Same CDS sequence)	XM_005223521.3 , XM_005192920.3 , XM_005223521.4
Accession Version	XM_005192920.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192920.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTTT 
GCCCAGGACT TACAGTGGAA TCAGTTTCAG TACCACTTCT CCAAGGCTCG CCAGTTGGTG 
AAAAGAACCA ATGCCTCACT ATTTGGTTCC CATGGCTTTG TCCGATTCCT AGAGTGCCAG 
GTGCTAATTT TACAGAAAAT GCCAGAAGAT GTCTTCCTGC AAACTCTCGC GGAGACTCGC 
CGCCAAACCC TCACGTACTT TAAGGAGTTT TTCTCTCGAT GTGTGACCTG CCCTGTCTAT 
CACCAGTGGG TCTCTGAGCT TAAAGCCTTT GTAATGAGAA ATGGAAAGAT GTCCTTGACC 
AACATCATCA GACCTTTTGA CACCTGCCTG ACCAGGATTT GGATAGAGGG AGAATTCCTG 
GAGGAAAATA ACTCCTTTGA ACAAAATTTG GAAATACAGA GATTTGTCAG AGAGGCTGAT 
GTCAACAAGA AGGATAAAGA AGAAATTCAA GAATGTATCT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005192977.2
CDS	16..4920
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005192920.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005192920.3

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTTT 
GCCCAGGACT TACAGTGGAA TCAGTTTCAG TACCACTTCT CCAAGGCTCG CCAGTTGGTG 
AAAAGAACCA ATGCCTCACT ATTTGGTTCC CATGGCTTTG TCCGATTCCT AGAGTGCCAG 
GTGCTAATTT TACAGAAAAT GCCAGAAGAT GTCTTCCTGC AAACTCTCGC GGAGACTCGC 
CGCCAAACCC TCACGTACTT TAAGGAGTTT TTCTCTCGAT GTGTGACCTG CCCTGTCTAT 
CACCAGTGGG TCTCTGAGCT TAAAGCCTTT GTAATGAGAA ATGGAAAGAT GTCCTTGACC 
AACATCATCA GACCTTTTGA CACCTGCCTG ACCAGGATTT GGATAGAGGG AGAATTCCTG 
GAGGAAAATA ACTCCTTTGA ACAAAATTTG GAAATACAGA GATTTGTCAG AGAGGCTGAT 
GTCAACAAGA AGGATAAAGA AGAAATTCAA GAATGTATCT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005223521.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223578.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.

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CloneID	OBa52049
Clone ID Related Accession (Same CDS sequence)	XM_005223521.3 , XM_005192920.3 , XM_005223521.4
Accession Version	XM_005223521.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223521.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTTT 
GCCCAGGACT TACAGTGGAA TCAGTTTCAG TACCACTTCT CCAAGGCTCG CCAGTTGGTG 
AAAAGAACCA ATGCCTCACT ATTTGGTTCC CATGGCTTTG TCCGATTCCT AGAGTGCCAG 
GTGCTAATTT TACAGAAAAT GCCAGAAGAT GTCTTCCTGC AAACTCTCGC GGAGACTCGC 
CGCCAAACCC TCACGTACTT TAAGGAGTTT TTCTCTCGAT GTGTGACCTG CCCTGTCTAT 
CACCAGTGGG TCTCTGAGCT TAAAGCCTTT GTAATGAGAA ATGGAAAGAT GTCCTTGACC 
AACATCATCA GACCTTTTGA CACCTGCCTG ACCAGGATTT GGATAGAGGG AGAATTCCTG 
GAGGAAAATA ACTCCTTTGA ACAAAATTTG GAAATACAGA GATTTGTCAG AGAGGCTGAT 
GTCAACAAGA AGGATAAAGA AGAAATTCAA GAATGTATCT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223578.2
CDS	711..5615
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005223521.4
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223521.4

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTTT 
GCCCAGGACT TACAGTGGAA TCAGTTTCAG TACCACTTCT CCAAGGCTCG CCAGTTGGTG 
AAAAGAACCA ATGCCTCACT ATTTGGTTCC CATGGCTTTG TCCGATTCCT AGAGTGCCAG 
GTGCTAATTT TACAGAAAAT GCCAGAAGAT GTCTTCCTGC AAACTCTCGC GGAGACTCGC 
CGCCAAACCC TCACGTACTT TAAGGAGTTT TTCTCTCGAT GTGTGACCTG CCCTGTCTAT 
CACCAGTGGG TCTCTGAGCT TAAAGCCTTT GTAATGAGAA ATGGAAAGAT GTCCTTGACC 
AACATCATCA GACCTTTTGA CACCTGCCTG ACCAGGATTT GGATAGAGGG AGAATTCCTG 
GAGGAAAATA ACTCCTTTGA ACAAAATTTG GAAATACAGA GATTTGTCAG AGAGGCTGAT 
GTCAACAAGA AGGATAAAGA AGAAATTCAA GAATGTATCT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005223523.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223580.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.

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CloneID	OBa52050
Clone ID Related Accession (Same CDS sequence)	XM_005223523.3 , XM_005223523.4 , XM_005192922.3
Accession Version	XM_005223523.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGATGTGCT CTGTTTTGGA 
GGAGATATCC TAAATATCAC AGGGAATGTG CTCCTAGCAC TCTGGACAGT GGAACAAAAT 
CAACTGAGTG ACATCATTAC TCTGGTGGCA AAATGCAGTC TGGAGATACA GGAGAAATTT 
GGGATCTACC ATACCAGAGA AGGCCAGGAC CTGCAGCTAA AGATAGGTCT ATCTGCAGGT 
CGGATTTCCC AGGTTATTGT TGGAGATGAG CAGCGCCAGT ACCTCTTGGT GATTGGCCGG 
GATGTAGATG GTGTGCAGTT AGCTCAACGG TTGGCTCAAG CAAATGAGAT TGTCCTGTCC 
TGGAACTGCT GGAAGCTCTG TGAGCAGTAC ATGTTTGAAG TGGAAATCAT GCGGGAGGAT 
GAAGCTGTGA AGTTAAGAGA TATGCGGATC ATTGACCCAT TTGATTTTGA TGAGCATTTT 
TACAAGTGCC TCGATTATCT GCCACATTAC CGGACAAGTG CTGACATTCT AAGAGCCGCC 
CTGGAGTTGG ATCCAGATTC TGCACTTGAG CAAACCCTGC GGAAGCACAT AATGAAAAAC 
CTTTTGAAGA AGATTGATGA AGGCCAGCCT ATGGAGTATA TATCCGAATT CCGCACTGTG 
ACTATGGTTT TGGTCAGCCT CGAGTTCCAC AAGACAGCAT GGGTGTTGCA TTTGTGCCAT 
CTTATCCAGG AGGCTGCGTT ATACATCTCC ACAGTGATAG AGACAGGGGG TGGCCAGCTG 
AGCCGGATCT TTATGTTTGA GACAGGCTGC ATGTTCCTCT GTGTTTTCGG CCTACCTGGG 
GATAAGAAGC CAGACGAGTG TGCACATGCC CTGGAGAGCT CCTTCAGCAT CTTCAGCTTC 
TGCTGGGAGA ATCTTGCTGA GACCAAGCTT GTTTCCATCA GTATCACCAA TGGACCAGTA 
TTCTGTGGCA TGGTTGGGGC AGTTTCAAGA CACGAATATA CAGTTATTGG CCCAAAAGTA 
AGCCTCGTGG CAAGAATGAT AACTGCTTAT CCAGGTTTGG TGTCCTGTGA TGAGGTAACA 
TATCTGAGAT CTATGCTACC TGCTTACAAC TTCAAGAAAC TCCCAGAGAA AATGATGAAA 
AATATCTCTA ACCCAGGGAA GATGTATGAA TACGTTGGCC ATAGAAGATG TATAATGTTT 
GGGAAGAGAC ATTTGGCCAC AGAGAGAAAT AAAAATCACT CTTTGCTAGA CCGGGAGAAA 
GAACTGGAAG CTTTCCGAAT GGCACAGCAG GAATGTTTGC ACCAGAAGAA GGGACAGGCA 
GTTCTATATG AAGGTGGAAA AGGCAGTGGA AAAAGTCAGC TCTTGGCTGA AATAAACTTC 
CTGGCCCAGA AGGAAGGGCA CAGGGTGTTG CCGTTGAAGC TGAAGAAAGC GGATTCCAAG 
CAGTGCTTCT ACGCCATCCA GACCCTCATG GCCATCTTCT TCGAGATTGA TACATGCCCA 
GCCCATTACC GGCAAGAGCG CCTCCACAGA CAGCTCAGTG GAATAGTGGA GGAGCCACTT 
CACTGCCTTT TTAATGACCT CTTCTTTGTG AAGTTTCCCT TATCCTTCAA AGTTTCACGC 
ATGGATGACC TGGCCAGACA ACAGAAGATT AAAGCATGTT TTTTTGAAGT GCTTGAGAAG 
GCAGTAAGGG AAACACCACT GATTTTCCTC ATTGACGAGG CCCAGTATAT GGATGCAGCT 
TCCTGGGAAT TTTTGGGAAC ATTGCTCTCC GGTGTGCCTA TCATCATGGT GATGACGTTG 
ACCACTACCT CCACCCTGTG TGGCCAGGCC CAGCACTTCC TGCAGAGCCC TCCAGCTGTC 
CTGGTGCCCA TTTCAAAGTT GGCAGCGACC TCACTGTTGA GAATGGCATG TTGGGAACTG 
GGGGTGGTGA GCATCCCCCA AGAGCTGCAG ACCTACCTTC TCCGCAGGTG CTTTGGAAAC 
CCCCTTTATT GTGAGGTCTT ATGCCAGGAT CTTCTCTCTA AAAACGTATT GTGCTTTCAT 
GACCTCCAAA AGGAGGAAGT GGAGAACAGC AAGTGGGAGA CCCTCTCAGC CAATGCCATG 
AAATCCACCA TGTACAGTGT TTCTCCTGCC AGCTCTGAAG ACCAGGAACT TTATGTCTGC 
ACAGTCAAGG ATGATGTGAA CTTGGATACA GTGCTTCTTC CACCGTTATT AAAAGAAATA 
GCAGTGAACC AAATAGATCA ACTGAGCCCA GAGGAACAGT CGCTGGTTAA ATGTGCTGCA 
GTCATTGGTC ACTTCTTCCA CATAGATCTG CTGCAGCACC TCCTGCCTGG CTGGAGTAAG 
AATAAGCTAC TTGAGGTACT GAAGGATCTT GTGGATATGC ACGTGCTCCA CTGGTGCAAT 
AAGGGCCAAG AGCTTCCTGC TGAGCCAATA TTAGTGCCTT CCTCTGTCGA GACTGAGCAT 
ACCAAAGAGA CGACAAAGTC AGATGCTGAC TCTCCTCTAA AGCTACAAGA GGAACTATCC 
CTACCTCAGA TGGAGGTGCT TGAATTTGGA GTGCCTCTTT TGCGGGAAGG GGCTTGGGAG 
CTGTGGCCCA AGGCACAGCA AACAGCCCTA CACCTTGAAT GTGCCTGCTT TCTTCGAGAT 
CTGGCCTGCC GCTGTGGGAG CTGTCATGGG GGAGACTTTG TCCCCTTCCA CCGTTTCGCC 
ATCTGTTCCA CTAAGAGCTC CAGCAGGACC TCCCGATTCT GTAGCTACAA AGACACTGGC 
TCGATACTAA CACAAGTGAT CACAGACAGA TTGCAGCTGC CTTCTCCCCA AGACACTTTT 
CAGTTCCAGA CAGAATCATC CAGAAGTCAG GAGGCCTTCA CAAGGGAATA CTGCAGAACA 
GAGGAAGAGT TCCTGGATAA AGTGAACAGG AAACTGGCTC AGACCAACCC CAAGAAAGGC 
ATGTTGACCA CGAAGCCCTG CCACTGTAGG GAAGTCCTGA AGTTAGTGCT CTCGCCCCTC 
ACCCAGCACT GCCTGGTCAT TGGAGAAACC ACGTGTGCAT TTTATTACCT GCTGGAATCT 
GCCGCTGCCT CCTTGGATCT GTCAGATAAT TACATGGCCT TCTTTTATTT GAGGAAGGCA 
AGCAGTCTTC TGGGCCAACC CTCCGCATTC TCGTTTTTTA AAAAGCACAA GGTGAAGATC 
TGTCAGTTTG AAGAAGCCAC TTTCTGTCGT CTCCAGTCAG AGGTCTGTTT CAACATGGGA 
CAGATCACCC TGGCCAAAAA ACTGGCTAGG CGAGCCCTTC GTCTGCTGAA AAGGAGTTTT 
CCCTGGACCT GGCTTGGTGT CATTTTCCAA ACATTTCTAG AAAAATGCTG GTATTCCTGT 
TCCCTGAGTC AACCTCGAAA CAACCCTAGT GAGAAGAAGA AGAAGCTGGC AGTCCTGCAG 
CAGCAGATGC ACTGTCTCTC CCTGCTCCGG CAGCTCTACA GCCTGGAGGC CACAGCCAGC 
AGCCTCAGGT TTGCCTGCCT GGCCACTCTT ATGCAGAAGA ATTCAGTCAA GGAGTTTCCT 
GGTGAAGCCC AGGTTGTCTC TACCTATGTG GCCCTCTCCC AGTTCTCCCA GAACGTGGGT 
GACAAGGAGA AGTGGCTGTA CCATGAGCAG CTGGCCATTC AGAAAAGCAG CCTGTGCTGG 
TTCTCCAGGG AGGGACTGTT GGCCACAGCC CAGCTCATGC AGGCCTTGGC CTACACCAAA 
CTCTGCCTTG GGCATCTCGA CTTCTCCATC AAGCTGGGTT TTCAAGCTCA TGAGATATGC 
AGACACCTCA AGAAACCAGC TCTGGAGAAC CTGGTTCTCT CAGTTCTCTT CAGATCTGCA 
TTTCTGAAGA AGAAGTTTGA TCTGTGTGTC CACGTGCTGG AGAGTCAGTG GGTGCTCATT 
TCCCAGAGTC ATGATGTCCT TGGCCGGGCC TTCTTCTATT CTGCTTGCTT AGATCTGCTG 
CTCTACGGAA AAGGATGGCT GTGTCGGCCC TTTGGGGAAT GTCTGCATTT CATTCAACGC 
TATGAGCACA GCTGCATTCT AAATTCTCAG AGCAGTGTCA TGCTGGGGGT CCACTCCTCT 
CTGGCCATGT GGTTTGCCCA GGACTTACAG TGGAATCAGT TTCAGTACCA CTTCTCCAAG 
GCTCGCCAGT TGGTGAAAAG AACCAATGCC TCACTATTTG GTTCCCATGG CTTTGTCCGA 
TTCCTAGAGT GCCAGGTGCT AATTTTACAG AAAATGCCAG AAGATGTCTT CCTGCAAACT 
CTCGCGGAGA CTCGCCGCCA AACCCTCACG TACTTTAAGG AGTTTTTCTC TCGATGTGTG 
ACCTGCCCTG TCTATCACCA GTGGGTCTCT GAGCTTAAAG CCTTTGTAAT GAGAAATGGA 
AAGATGTCCT TGACCAACAT CATCAGACCT TTTGACACCT GCCTGACCAG GATTTGGATA 
GAGGGAGAAT TCCTGGAGGA AAATAACTCC TTTGAACAAA ATTTGGAAAT ACAGAGATTT 
GTCAGAGAGG CTGATGTCAA CAAGAAGGAT AAAGAAGAAA TTCAAGAATG TATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223580.2
CDS	16..4815
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTDVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005223523.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223523.3

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGATGTGCT CTGTTTTGGA 
GGAGATATCC TAAATATCAC AGGGAATGTG CTCCTAGCAC TCTGGACAGT GGAACAAAAT 
CAACTGAGTG ACATCATTAC TCTGGTGGCA AAATGCAGTC TGGAGATACA GGAGAAATTT 
GGGATCTACC ATACCAGAGA AGGCCAGGAC CTGCAGCTAA AGATAGGTCT ATCTGCAGGT 
CGGATTTCCC AGGTTATTGT TGGAGATGAG CAGCGCCAGT ACCTCTTGGT GATTGGCCGG 
GATGTAGATG GTGTGCAGTT AGCTCAACGG TTGGCTCAAG CAAATGAGAT TGTCCTGTCC 
TGGAACTGCT GGAAGCTCTG TGAGCAGTAC ATGTTTGAAG TGGAAATCAT GCGGGAGGAT 
GAAGCTGTGA AGTTAAGAGA TATGCGGATC ATTGACCCAT TTGATTTTGA TGAGCATTTT 
TACAAGTGCC TCGATTATCT GCCACATTAC CGGACAAGTG CTGACATTCT AAGAGCCGCC 
CTGGAGTTGG ATCCAGATTC TGCACTTGAG CAAACCCTGC GGAAGCACAT AATGAAAAAC 
CTTTTGAAGA AGATTGATGA AGGCCAGCCT ATGGAGTATA TATCCGAATT CCGCACTGTG 
ACTATGGTTT TGGTCAGCCT CGAGTTCCAC AAGACAGCAT GGGTGTTGCA TTTGTGCCAT 
CTTATCCAGG AGGCTGCGTT ATACATCTCC ACAGTGATAG AGACAGGGGG TGGCCAGCTG 
AGCCGGATCT TTATGTTTGA GACAGGCTGC ATGTTCCTCT GTGTTTTCGG CCTACCTGGG 
GATAAGAAGC CAGACGAGTG TGCACATGCC CTGGAGAGCT CCTTCAGCAT CTTCAGCTTC 
TGCTGGGAGA ATCTTGCTGA GACCAAGCTT GTTTCCATCA GTATCACCAA TGGACCAGTA 
TTCTGTGGCA TGGTTGGGGC AGTTTCAAGA CACGAATATA CAGTTATTGG CCCAAAAGTA 
AGCCTCGTGG CAAGAATGAT AACTGCTTAT CCAGGTTTGG TGTCCTGTGA TGAGGTAACA 
TATCTGAGAT CTATGCTACC TGCTTACAAC TTCAAGAAAC TCCCAGAGAA AATGATGAAA 
AATATCTCTA ACCCAGGGAA GATGTATGAA TACGTTGGCC ATAGAAGATG TATAATGTTT 
GGGAAGAGAC ATTTGGCCAC AGAGAGAAAT AAAAATCACT CTTTGCTAGA CCGGGAGAAA 
GAACTGGAAG CTTTCCGAAT GGCACAGCAG GAATGTTTGC ACCAGAAGAA GGGACAGGCA 
GTTCTATATG AAGGTGGAAA AGGCAGTGGA AAAAGTCAGC TCTTGGCTGA AATAAACTTC 
CTGGCCCAGA AGGAAGGGCA CAGGGTGTTG CCGTTGAAGC TGAAGAAAGC GGATTCCAAG 
CAGTGCTTCT ACGCCATCCA GACCCTCATG GCCATCTTCT TCGAGATTGA TACATGCCCA 
GCCCATTACC GGCAAGAGCG CCTCCACAGA CAGCTCAGTG GAATAGTGGA GGAGCCACTT 
CACTGCCTTT TTAATGACCT CTTCTTTGTG AAGTTTCCCT TATCCTTCAA AGTTTCACGC 
ATGGATGACC TGGCCAGACA ACAGAAGATT AAAGCATGTT TTTTTGAAGT GCTTGAGAAG 
GCAGTAAGGG AAACACCACT GATTTTCCTC ATTGACGAGG CCCAGTATAT GGATGCAGCT 
TCCTGGGAAT TTTTGGGAAC ATTGCTCTCC GGTGTGCCTA TCATCATGGT GATGACGTTG 
ACCACTACCT CCACCCTGTG TGGCCAGGCC CAGCACTTCC TGCAGAGCCC TCCAGCTGTC 
CTGGTGCCCA TTTCAAAGTT GGCAGCGACC TCACTGTTGA GAATGGCATG TTGGGAACTG 
GGGGTGGTGA GCATCCCCCA AGAGCTGCAG ACCTACCTTC TCCGCAGGTG CTTTGGAAAC 
CCCCTTTATT GTGAGGTCTT ATGCCAGGAT CTTCTCTCTA AAAACGTATT GTGCTTTCAT 
GACCTCCAAA AGGAGGAAGT GGAGAACAGC AAGTGGGAGA CCCTCTCAGC CAATGCCATG 
AAATCCACCA TGTACAGTGT TTCTCCTGCC AGCTCTGAAG ACCAGGAACT TTATGTCTGC 
ACAGTCAAGG ATGATGTGAA CTTGGATACA GTGCTTCTTC CACCGTTATT AAAAGAAATA 
GCAGTGAACC AAATAGATCA ACTGAGCCCA GAGGAACAGT CGCTGGTTAA ATGTGCTGCA 
GTCATTGGTC ACTTCTTCCA CATAGATCTG CTGCAGCACC TCCTGCCTGG CTGGAGTAAG 
AATAAGCTAC TTGAGGTACT GAAGGATCTT GTGGATATGC ACGTGCTCCA CTGGTGCAAT 
AAGGGCCAAG AGCTTCCTGC TGAGCCAATA TTAGTGCCTT CCTCTGTCGA GACTGAGCAT 
ACCAAAGAGA CGACAAAGTC AGATGCTGAC TCTCCTCTAA AGCTACAAGA GGAACTATCC 
CTACCTCAGA TGGAGGTGCT TGAATTTGGA GTGCCTCTTT TGCGGGAAGG GGCTTGGGAG 
CTGTGGCCCA AGGCACAGCA AACAGCCCTA CACCTTGAAT GTGCCTGCTT TCTTCGAGAT 
CTGGCCTGCC GCTGTGGGAG CTGTCATGGG GGAGACTTTG TCCCCTTCCA CCGTTTCGCC 
ATCTGTTCCA CTAAGAGCTC CAGCAGGACC TCCCGATTCT GTAGCTACAA AGACACTGGC 
TCGATACTAA CACAAGTGAT CACAGACAGA TTGCAGCTGC CTTCTCCCCA AGACACTTTT 
CAGTTCCAGA CAGAATCATC CAGAAGTCAG GAGGCCTTCA CAAGGGAATA CTGCAGAACA 
GAGGAAGAGT TCCTGGATAA AGTGAACAGG AAACTGGCTC AGACCAACCC CAAGAAAGGC 
ATGTTGACCA CGAAGCCCTG CCACTGTAGG GAAGTCCTGA AGTTAGTGCT CTCGCCCCTC 
ACCCAGCACT GCCTGGTCAT TGGAGAAACC ACGTGTGCAT TTTATTACCT GCTGGAATCT 
GCCGCTGCCT CCTTGGATCT GTCAGATAAT TACATGGCCT TCTTTTATTT GAGGAAGGCA 
AGCAGTCTTC TGGGCCAACC CTCCGCATTC TCGTTTTTTA AAAAGCACAA GGTGAAGATC 
TGTCAGTTTG AAGAAGCCAC TTTCTGTCGT CTCCAGTCAG AGGTCTGTTT CAACATGGGA 
CAGATCACCC TGGCCAAAAA ACTGGCTAGG CGAGCCCTTC GTCTGCTGAA AAGGAGTTTT 
CCCTGGACCT GGCTTGGTGT CATTTTCCAA ACATTTCTAG AAAAATGCTG GTATTCCTGT 
TCCCTGAGTC AACCTCGAAA CAACCCTAGT GAGAAGAAGA AGAAGCTGGC AGTCCTGCAG 
CAGCAGATGC ACTGTCTCTC CCTGCTCCGG CAGCTCTACA GCCTGGAGGC CACAGCCAGC 
AGCCTCAGGT TTGCCTGCCT GGCCACTCTT ATGCAGAAGA ATTCAGTCAA GGAGTTTCCT 
GGTGAAGCCC AGGTTGTCTC TACCTATGTG GCCCTCTCCC AGTTCTCCCA GAACGTGGGT 
GACAAGGAGA AGTGGCTGTA CCATGAGCAG CTGGCCATTC AGAAAAGCAG CCTGTGCTGG 
TTCTCCAGGG AGGGACTGTT GGCCACAGCC CAGCTCATGC AGGCCTTGGC CTACACCAAA 
CTCTGCCTTG GGCATCTCGA CTTCTCCATC AAGCTGGGTT TTCAAGCTCA TGAGATATGC 
AGACACCTCA AGAAACCAGC TCTGGAGAAC CTGGTTCTCT CAGTTCTCTT CAGATCTGCA 
TTTCTGAAGA AGAAGTTTGA TCTGTGTGTC CACGTGCTGG AGAGTCAGTG GGTGCTCATT 
TCCCAGAGTC ATGATGTCCT TGGCCGGGCC TTCTTCTATT CTGCTTGCTT AGATCTGCTG 
CTCTACGGAA AAGGATGGCT GTGTCGGCCC TTTGGGGAAT GTCTGCATTT CATTCAACGC 
TATGAGCACA GCTGCATTCT AAATTCTCAG AGCAGTGTCA TGCTGGGGGT CCACTCCTCT 
CTGGCCATGT GGTTTGCCCA GGACTTACAG TGGAATCAGT TTCAGTACCA CTTCTCCAAG 
GCTCGCCAGT TGGTGAAAAG AACCAATGCC TCACTATTTG GTTCCCATGG CTTTGTCCGA 
TTCCTAGAGT GCCAGGTGCT AATTTTACAG AAAATGCCAG AAGATGTCTT CCTGCAAACT 
CTCGCGGAGA CTCGCCGCCA AACCCTCACG TACTTTAAGG AGTTTTTCTC TCGATGTGTG 
ACCTGCCCTG TCTATCACCA GTGGGTCTCT GAGCTTAAAG CCTTTGTAAT GAGAAATGGA 
AAGATGTCCT TGACCAACAT CATCAGACCT TTTGACACCT GCCTGACCAG GATTTGGATA 
GAGGGAGAAT TCCTGGAGGA AAATAACTCC TTTGAACAAA ATTTGGAAAT ACAGAGATTT 
GTCAGAGAGG CTGATGTCAA CAAGAAGGAT AAAGAAGAAA TTCAAGAATG TATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005223523.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223580.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52050
Clone ID Related Accession (Same CDS sequence)	XM_005223523.3 , XM_005223523.4 , XM_005192922.3
Accession Version	XM_005223523.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223523.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGATGTGCT CTGTTTTGGA 
GGAGATATCC TAAATATCAC AGGGAATGTG CTCCTAGCAC TCTGGACAGT GGAACAAAAT 
CAACTGAGTG ACATCATTAC TCTGGTGGCA AAATGCAGTC TGGAGATACA GGAGAAATTT 
GGGATCTACC ATACCAGAGA AGGCCAGGAC CTGCAGCTAA AGATAGGTCT ATCTGCAGGT 
CGGATTTCCC AGGTTATTGT TGGAGATGAG CAGCGCCAGT ACCTCTTGGT GATTGGCCGG 
GATGTAGATG GTGTGCAGTT AGCTCAACGG TTGGCTCAAG CAAATGAGAT TGTCCTGTCC 
TGGAACTGCT GGAAGCTCTG TGAGCAGTAC ATGTTTGAAG TGGAAATCAT GCGGGAGGAT 
GAAGCTGTGA AGTTAAGAGA TATGCGGATC ATTGACCCAT TTGATTTTGA TGAGCATTTT 
TACAAGTGCC TCGATTATCT GCCACATTAC CGGACAAGTG CTGACATTCT AAGAGCCGCC 
CTGGAGTTGG ATCCAGATTC TGCACTTGAG CAAACCCTGC GGAAGCACAT AATGAAAAAC 
CTTTTGAAGA AGATTGATGA AGGCCAGCCT ATGGAGTATA TATCCGAATT CCGCACTGTG 
ACTATGGTTT TGGTCAGCCT CGAGTTCCAC AAGACAGCAT GGGTGTTGCA TTTGTGCCAT 
CTTATCCAGG AGGCTGCGTT ATACATCTCC ACAGTGATAG AGACAGGGGG TGGCCAGCTG 
AGCCGGATCT TTATGTTTGA GACAGGCTGC ATGTTCCTCT GTGTTTTCGG CCTACCTGGG 
GATAAGAAGC CAGACGAGTG TGCACATGCC CTGGAGAGCT CCTTCAGCAT CTTCAGCTTC 
TGCTGGGAGA ATCTTGCTGA GACCAAGCTT GTTTCCATCA GTATCACCAA TGGACCAGTA 
TTCTGTGGCA TGGTTGGGGC AGTTTCAAGA CACGAATATA CAGTTATTGG CCCAAAAGTA 
AGCCTCGTGG CAAGAATGAT AACTGCTTAT CCAGGTTTGG TGTCCTGTGA TGAGGTAACA 
TATCTGAGAT CTATGCTACC TGCTTACAAC TTCAAGAAAC TCCCAGAGAA AATGATGAAA 
AATATCTCTA ACCCAGGGAA GATGTATGAA TACGTTGGCC ATAGAAGATG TATAATGTTT 
GGGAAGAGAC ATTTGGCCAC AGAGAGAAAT AAAAATCACT CTTTGCTAGA CCGGGAGAAA 
GAACTGGAAG CTTTCCGAAT GGCACAGCAG GAATGTTTGC ACCAGAAGAA GGGACAGGCA 
GTTCTATATG AAGGTGGAAA AGGCAGTGGA AAAAGTCAGC TCTTGGCTGA AATAAACTTC 
CTGGCCCAGA AGGAAGGGCA CAGGGTGTTG CCGTTGAAGC TGAAGAAAGC GGATTCCAAG 
CAGTGCTTCT ACGCCATCCA GACCCTCATG GCCATCTTCT TCGAGATTGA TACATGCCCA 
GCCCATTACC GGCAAGAGCG CCTCCACAGA CAGCTCAGTG GAATAGTGGA GGAGCCACTT 
CACTGCCTTT TTAATGACCT CTTCTTTGTG AAGTTTCCCT TATCCTTCAA AGTTTCACGC 
ATGGATGACC TGGCCAGACA ACAGAAGATT AAAGCATGTT TTTTTGAAGT GCTTGAGAAG 
GCAGTAAGGG AAACACCACT GATTTTCCTC ATTGACGAGG CCCAGTATAT GGATGCAGCT 
TCCTGGGAAT TTTTGGGAAC ATTGCTCTCC GGTGTGCCTA TCATCATGGT GATGACGTTG 
ACCACTACCT CCACCCTGTG TGGCCAGGCC CAGCACTTCC TGCAGAGCCC TCCAGCTGTC 
CTGGTGCCCA TTTCAAAGTT GGCAGCGACC TCACTGTTGA GAATGGCATG TTGGGAACTG 
GGGGTGGTGA GCATCCCCCA AGAGCTGCAG ACCTACCTTC TCCGCAGGTG CTTTGGAAAC 
CCCCTTTATT GTGAGGTCTT ATGCCAGGAT CTTCTCTCTA AAAACGTATT GTGCTTTCAT 
GACCTCCAAA AGGAGGAAGT GGAGAACAGC AAGTGGGAGA CCCTCTCAGC CAATGCCATG 
AAATCCACCA TGTACAGTGT TTCTCCTGCC AGCTCTGAAG ACCAGGAACT TTATGTCTGC 
ACAGTCAAGG ATGATGTGAA CTTGGATACA GTGCTTCTTC CACCGTTATT AAAAGAAATA 
GCAGTGAACC AAATAGATCA ACTGAGCCCA GAGGAACAGT CGCTGGTTAA ATGTGCTGCA 
GTCATTGGTC ACTTCTTCCA CATAGATCTG CTGCAGCACC TCCTGCCTGG CTGGAGTAAG 
AATAAGCTAC TTGAGGTACT GAAGGATCTT GTGGATATGC ACGTGCTCCA CTGGTGCAAT 
AAGGGCCAAG AGCTTCCTGC TGAGCCAATA TTAGTGCCTT CCTCTGTCGA GACTGAGCAT 
ACCAAAGAGA CGACAAAGTC AGATGCTGAC TCTCCTCTAA AGCTACAAGA GGAACTATCC 
CTACCTCAGA TGGAGGTGCT TGAATTTGGA GTGCCTCTTT TGCGGGAAGG GGCTTGGGAG 
CTGTGGCCCA AGGCACAGCA AACAGCCCTA CACCTTGAAT GTGCCTGCTT TCTTCGAGAT 
CTGGCCTGCC GCTGTGGGAG CTGTCATGGG GGAGACTTTG TCCCCTTCCA CCGTTTCGCC 
ATCTGTTCCA CTAAGAGCTC CAGCAGGACC TCCCGATTCT GTAGCTACAA AGACACTGGC 
TCGATACTAA CACAAGTGAT CACAGACAGA TTGCAGCTGC CTTCTCCCCA AGACACTTTT 
CAGTTCCAGA CAGAATCATC CAGAAGTCAG GAGGCCTTCA CAAGGGAATA CTGCAGAACA 
GAGGAAGAGT TCCTGGATAA AGTGAACAGG AAACTGGCTC AGACCAACCC CAAGAAAGGC 
ATGTTGACCA CGAAGCCCTG CCACTGTAGG GAAGTCCTGA AGTTAGTGCT CTCGCCCCTC 
ACCCAGCACT GCCTGGTCAT TGGAGAAACC ACGTGTGCAT TTTATTACCT GCTGGAATCT 
GCCGCTGCCT CCTTGGATCT GTCAGATAAT TACATGGCCT TCTTTTATTT GAGGAAGGCA 
AGCAGTCTTC TGGGCCAACC CTCCGCATTC TCGTTTTTTA AAAAGCACAA GGTGAAGATC 
TGTCAGTTTG AAGAAGCCAC TTTCTGTCGT CTCCAGTCAG AGGTCTGTTT CAACATGGGA 
CAGATCACCC TGGCCAAAAA ACTGGCTAGG CGAGCCCTTC GTCTGCTGAA AAGGAGTTTT 
CCCTGGACCT GGCTTGGTGT CATTTTCCAA ACATTTCTAG AAAAATGCTG GTATTCCTGT 
TCCCTGAGTC AACCTCGAAA CAACCCTAGT GAGAAGAAGA AGAAGCTGGC AGTCCTGCAG 
CAGCAGATGC ACTGTCTCTC CCTGCTCCGG CAGCTCTACA GCCTGGAGGC CACAGCCAGC 
AGCCTCAGGT TTGCCTGCCT GGCCACTCTT ATGCAGAAGA ATTCAGTCAA GGAGTTTCCT 
GGTGAAGCCC AGGTTGTCTC TACCTATGTG GCCCTCTCCC AGTTCTCCCA GAACGTGGGT 
GACAAGGAGA AGTGGCTGTA CCATGAGCAG CTGGCCATTC AGAAAAGCAG CCTGTGCTGG 
TTCTCCAGGG AGGGACTGTT GGCCACAGCC CAGCTCATGC AGGCCTTGGC CTACACCAAA 
CTCTGCCTTG GGCATCTCGA CTTCTCCATC AAGCTGGGTT TTCAAGCTCA TGAGATATGC 
AGACACCTCA AGAAACCAGC TCTGGAGAAC CTGGTTCTCT CAGTTCTCTT CAGATCTGCA 
TTTCTGAAGA AGAAGTTTGA TCTGTGTGTC CACGTGCTGG AGAGTCAGTG GGTGCTCATT 
TCCCAGAGTC ATGATGTCCT TGGCCGGGCC TTCTTCTATT CTGCTTGCTT AGATCTGCTG 
CTCTACGGAA AAGGATGGCT GTGTCGGCCC TTTGGGGAAT GTCTGCATTT CATTCAACGC 
TATGAGCACA GCTGCATTCT AAATTCTCAG AGCAGTGTCA TGCTGGGGGT CCACTCCTCT 
CTGGCCATGT GGTTTGCCCA GGACTTACAG TGGAATCAGT TTCAGTACCA CTTCTCCAAG 
GCTCGCCAGT TGGTGAAAAG AACCAATGCC TCACTATTTG GTTCCCATGG CTTTGTCCGA 
TTCCTAGAGT GCCAGGTGCT AATTTTACAG AAAATGCCAG AAGATGTCTT CCTGCAAACT 
CTCGCGGAGA CTCGCCGCCA AACCCTCACG TACTTTAAGG AGTTTTTCTC TCGATGTGTG 
ACCTGCCCTG TCTATCACCA GTGGGTCTCT GAGCTTAAAG CCTTTGTAAT GAGAAATGGA 
AAGATGTCCT TGACCAACAT CATCAGACCT TTTGACACCT GCCTGACCAG GATTTGGATA 
GAGGGAGAAT TCCTGGAGGA AAATAACTCC TTTGAACAAA ATTTGGAAAT ACAGAGATTT 
GTCAGAGAGG CTGATGTCAA CAAGAAGGAT AAAGAAGAAA TTCAAGAATG TATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223580.2
CDS	711..5510
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTDVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005223523.4
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223523.4

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGATGTGCT CTGTTTTGGA 
GGAGATATCC TAAATATCAC AGGGAATGTG CTCCTAGCAC TCTGGACAGT GGAACAAAAT 
CAACTGAGTG ACATCATTAC TCTGGTGGCA AAATGCAGTC TGGAGATACA GGAGAAATTT 
GGGATCTACC ATACCAGAGA AGGCCAGGAC CTGCAGCTAA AGATAGGTCT ATCTGCAGGT 
CGGATTTCCC AGGTTATTGT TGGAGATGAG CAGCGCCAGT ACCTCTTGGT GATTGGCCGG 
GATGTAGATG GTGTGCAGTT AGCTCAACGG TTGGCTCAAG CAAATGAGAT TGTCCTGTCC 
TGGAACTGCT GGAAGCTCTG TGAGCAGTAC ATGTTTGAAG TGGAAATCAT GCGGGAGGAT 
GAAGCTGTGA AGTTAAGAGA TATGCGGATC ATTGACCCAT TTGATTTTGA TGAGCATTTT 
TACAAGTGCC TCGATTATCT GCCACATTAC CGGACAAGTG CTGACATTCT AAGAGCCGCC 
CTGGAGTTGG ATCCAGATTC TGCACTTGAG CAAACCCTGC GGAAGCACAT AATGAAAAAC 
CTTTTGAAGA AGATTGATGA AGGCCAGCCT ATGGAGTATA TATCCGAATT CCGCACTGTG 
ACTATGGTTT TGGTCAGCCT CGAGTTCCAC AAGACAGCAT GGGTGTTGCA TTTGTGCCAT 
CTTATCCAGG AGGCTGCGTT ATACATCTCC ACAGTGATAG AGACAGGGGG TGGCCAGCTG 
AGCCGGATCT TTATGTTTGA GACAGGCTGC ATGTTCCTCT GTGTTTTCGG CCTACCTGGG 
GATAAGAAGC CAGACGAGTG TGCACATGCC CTGGAGAGCT CCTTCAGCAT CTTCAGCTTC 
TGCTGGGAGA ATCTTGCTGA GACCAAGCTT GTTTCCATCA GTATCACCAA TGGACCAGTA 
TTCTGTGGCA TGGTTGGGGC AGTTTCAAGA CACGAATATA CAGTTATTGG CCCAAAAGTA 
AGCCTCGTGG CAAGAATGAT AACTGCTTAT CCAGGTTTGG TGTCCTGTGA TGAGGTAACA 
TATCTGAGAT CTATGCTACC TGCTTACAAC TTCAAGAAAC TCCCAGAGAA AATGATGAAA 
AATATCTCTA ACCCAGGGAA GATGTATGAA TACGTTGGCC ATAGAAGATG TATAATGTTT 
GGGAAGAGAC ATTTGGCCAC AGAGAGAAAT AAAAATCACT CTTTGCTAGA CCGGGAGAAA 
GAACTGGAAG CTTTCCGAAT GGCACAGCAG GAATGTTTGC ACCAGAAGAA GGGACAGGCA 
GTTCTATATG AAGGTGGAAA AGGCAGTGGA AAAAGTCAGC TCTTGGCTGA AATAAACTTC 
CTGGCCCAGA AGGAAGGGCA CAGGGTGTTG CCGTTGAAGC TGAAGAAAGC GGATTCCAAG 
CAGTGCTTCT ACGCCATCCA GACCCTCATG GCCATCTTCT TCGAGATTGA TACATGCCCA 
GCCCATTACC GGCAAGAGCG CCTCCACAGA CAGCTCAGTG GAATAGTGGA GGAGCCACTT 
CACTGCCTTT TTAATGACCT CTTCTTTGTG AAGTTTCCCT TATCCTTCAA AGTTTCACGC 
ATGGATGACC TGGCCAGACA ACAGAAGATT AAAGCATGTT TTTTTGAAGT GCTTGAGAAG 
GCAGTAAGGG AAACACCACT GATTTTCCTC ATTGACGAGG CCCAGTATAT GGATGCAGCT 
TCCTGGGAAT TTTTGGGAAC ATTGCTCTCC GGTGTGCCTA TCATCATGGT GATGACGTTG 
ACCACTACCT CCACCCTGTG TGGCCAGGCC CAGCACTTCC TGCAGAGCCC TCCAGCTGTC 
CTGGTGCCCA TTTCAAAGTT GGCAGCGACC TCACTGTTGA GAATGGCATG TTGGGAACTG 
GGGGTGGTGA GCATCCCCCA AGAGCTGCAG ACCTACCTTC TCCGCAGGTG CTTTGGAAAC 
CCCCTTTATT GTGAGGTCTT ATGCCAGGAT CTTCTCTCTA AAAACGTATT GTGCTTTCAT 
GACCTCCAAA AGGAGGAAGT GGAGAACAGC AAGTGGGAGA CCCTCTCAGC CAATGCCATG 
AAATCCACCA TGTACAGTGT TTCTCCTGCC AGCTCTGAAG ACCAGGAACT TTATGTCTGC 
ACAGTCAAGG ATGATGTGAA CTTGGATACA GTGCTTCTTC CACCGTTATT AAAAGAAATA 
GCAGTGAACC AAATAGATCA ACTGAGCCCA GAGGAACAGT CGCTGGTTAA ATGTGCTGCA 
GTCATTGGTC ACTTCTTCCA CATAGATCTG CTGCAGCACC TCCTGCCTGG CTGGAGTAAG 
AATAAGCTAC TTGAGGTACT GAAGGATCTT GTGGATATGC ACGTGCTCCA CTGGTGCAAT 
AAGGGCCAAG AGCTTCCTGC TGAGCCAATA TTAGTGCCTT CCTCTGTCGA GACTGAGCAT 
ACCAAAGAGA CGACAAAGTC AGATGCTGAC TCTCCTCTAA AGCTACAAGA GGAACTATCC 
CTACCTCAGA TGGAGGTGCT TGAATTTGGA GTGCCTCTTT TGCGGGAAGG GGCTTGGGAG 
CTGTGGCCCA AGGCACAGCA AACAGCCCTA CACCTTGAAT GTGCCTGCTT TCTTCGAGAT 
CTGGCCTGCC GCTGTGGGAG CTGTCATGGG GGAGACTTTG TCCCCTTCCA CCGTTTCGCC 
ATCTGTTCCA CTAAGAGCTC CAGCAGGACC TCCCGATTCT GTAGCTACAA AGACACTGGC 
TCGATACTAA CACAAGTGAT CACAGACAGA TTGCAGCTGC CTTCTCCCCA AGACACTTTT 
CAGTTCCAGA CAGAATCATC CAGAAGTCAG GAGGCCTTCA CAAGGGAATA CTGCAGAACA 
GAGGAAGAGT TCCTGGATAA AGTGAACAGG AAACTGGCTC AGACCAACCC CAAGAAAGGC 
ATGTTGACCA CGAAGCCCTG CCACTGTAGG GAAGTCCTGA AGTTAGTGCT CTCGCCCCTC 
ACCCAGCACT GCCTGGTCAT TGGAGAAACC ACGTGTGCAT TTTATTACCT GCTGGAATCT 
GCCGCTGCCT CCTTGGATCT GTCAGATAAT TACATGGCCT TCTTTTATTT GAGGAAGGCA 
AGCAGTCTTC TGGGCCAACC CTCCGCATTC TCGTTTTTTA AAAAGCACAA GGTGAAGATC 
TGTCAGTTTG AAGAAGCCAC TTTCTGTCGT CTCCAGTCAG AGGTCTGTTT CAACATGGGA 
CAGATCACCC TGGCCAAAAA ACTGGCTAGG CGAGCCCTTC GTCTGCTGAA AAGGAGTTTT 
CCCTGGACCT GGCTTGGTGT CATTTTCCAA ACATTTCTAG AAAAATGCTG GTATTCCTGT 
TCCCTGAGTC AACCTCGAAA CAACCCTAGT GAGAAGAAGA AGAAGCTGGC AGTCCTGCAG 
CAGCAGATGC ACTGTCTCTC CCTGCTCCGG CAGCTCTACA GCCTGGAGGC CACAGCCAGC 
AGCCTCAGGT TTGCCTGCCT GGCCACTCTT ATGCAGAAGA ATTCAGTCAA GGAGTTTCCT 
GGTGAAGCCC AGGTTGTCTC TACCTATGTG GCCCTCTCCC AGTTCTCCCA GAACGTGGGT 
GACAAGGAGA AGTGGCTGTA CCATGAGCAG CTGGCCATTC AGAAAAGCAG CCTGTGCTGG 
TTCTCCAGGG AGGGACTGTT GGCCACAGCC CAGCTCATGC AGGCCTTGGC CTACACCAAA 
CTCTGCCTTG GGCATCTCGA CTTCTCCATC AAGCTGGGTT TTCAAGCTCA TGAGATATGC 
AGACACCTCA AGAAACCAGC TCTGGAGAAC CTGGTTCTCT CAGTTCTCTT CAGATCTGCA 
TTTCTGAAGA AGAAGTTTGA TCTGTGTGTC CACGTGCTGG AGAGTCAGTG GGTGCTCATT 
TCCCAGAGTC ATGATGTCCT TGGCCGGGCC TTCTTCTATT CTGCTTGCTT AGATCTGCTG 
CTCTACGGAA AAGGATGGCT GTGTCGGCCC TTTGGGGAAT GTCTGCATTT CATTCAACGC 
TATGAGCACA GCTGCATTCT AAATTCTCAG AGCAGTGTCA TGCTGGGGGT CCACTCCTCT 
CTGGCCATGT GGTTTGCCCA GGACTTACAG TGGAATCAGT TTCAGTACCA CTTCTCCAAG 
GCTCGCCAGT TGGTGAAAAG AACCAATGCC TCACTATTTG GTTCCCATGG CTTTGTCCGA 
TTCCTAGAGT GCCAGGTGCT AATTTTACAG AAAATGCCAG AAGATGTCTT CCTGCAAACT 
CTCGCGGAGA CTCGCCGCCA AACCCTCACG TACTTTAAGG AGTTTTTCTC TCGATGTGTG 
ACCTGCCCTG TCTATCACCA GTGGGTCTCT GAGCTTAAAG CCTTTGTAAT GAGAAATGGA 
AAGATGTCCT TGACCAACAT CATCAGACCT TTTGACACCT GCCTGACCAG GATTTGGATA 
GAGGGAGAAT TCCTGGAGGA AAATAACTCC TTTGAACAAA ATTTGGAAAT ACAGAGATTT 
GTCAGAGAGG CTGATGTCAA CAAGAAGGAT AAAGAAGAAA TTCAAGAATG TATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005192922.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005192979.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.

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CloneID	OBa52050
Clone ID Related Accession (Same CDS sequence)	XM_005223523.3 , XM_005223523.4 , XM_005192922.3
Accession Version	XM_005192922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGATGTGCT CTGTTTTGGA 
GGAGATATCC TAAATATCAC AGGGAATGTG CTCCTAGCAC TCTGGACAGT GGAACAAAAT 
CAACTGAGTG ACATCATTAC TCTGGTGGCA AAATGCAGTC TGGAGATACA GGAGAAATTT 
GGGATCTACC ATACCAGAGA AGGCCAGGAC CTGCAGCTAA AGATAGGTCT ATCTGCAGGT 
CGGATTTCCC AGGTTATTGT TGGAGATGAG CAGCGCCAGT ACCTCTTGGT GATTGGCCGG 
GATGTAGATG GTGTGCAGTT AGCTCAACGG TTGGCTCAAG CAAATGAGAT TGTCCTGTCC 
TGGAACTGCT GGAAGCTCTG TGAGCAGTAC ATGTTTGAAG TGGAAATCAT GCGGGAGGAT 
GAAGCTGTGA AGTTAAGAGA TATGCGGATC ATTGACCCAT TTGATTTTGA TGAGCATTTT 
TACAAGTGCC TCGATTATCT GCCACATTAC CGGACAAGTG CTGACATTCT AAGAGCCGCC 
CTGGAGTTGG ATCCAGATTC TGCACTTGAG CAAACCCTGC GGAAGCACAT AATGAAAAAC 
CTTTTGAAGA AGATTGATGA AGGCCAGCCT ATGGAGTATA TATCCGAATT CCGCACTGTG 
ACTATGGTTT TGGTCAGCCT CGAGTTCCAC AAGACAGCAT GGGTGTTGCA TTTGTGCCAT 
CTTATCCAGG AGGCTGCGTT ATACATCTCC ACAGTGATAG AGACAGGGGG TGGCCAGCTG 
AGCCGGATCT TTATGTTTGA GACAGGCTGC ATGTTCCTCT GTGTTTTCGG CCTACCTGGG 
GATAAGAAGC CAGACGAGTG TGCACATGCC CTGGAGAGCT CCTTCAGCAT CTTCAGCTTC 
TGCTGGGAGA ATCTTGCTGA GACCAAGCTT GTTTCCATCA GTATCACCAA TGGACCAGTA 
TTCTGTGGCA TGGTTGGGGC AGTTTCAAGA CACGAATATA CAGTTATTGG CCCAAAAGTA 
AGCCTCGTGG CAAGAATGAT AACTGCTTAT CCAGGTTTGG TGTCCTGTGA TGAGGTAACA 
TATCTGAGAT CTATGCTACC TGCTTACAAC TTCAAGAAAC TCCCAGAGAA AATGATGAAA 
AATATCTCTA ACCCAGGGAA GATGTATGAA TACGTTGGCC ATAGAAGATG TATAATGTTT 
GGGAAGAGAC ATTTGGCCAC AGAGAGAAAT AAAAATCACT CTTTGCTAGA CCGGGAGAAA 
GAACTGGAAG CTTTCCGAAT GGCACAGCAG GAATGTTTGC ACCAGAAGAA GGGACAGGCA 
GTTCTATATG AAGGTGGAAA AGGCAGTGGA AAAAGTCAGC TCTTGGCTGA AATAAACTTC 
CTGGCCCAGA AGGAAGGGCA CAGGGTGTTG CCGTTGAAGC TGAAGAAAGC GGATTCCAAG 
CAGTGCTTCT ACGCCATCCA GACCCTCATG GCCATCTTCT TCGAGATTGA TACATGCCCA 
GCCCATTACC GGCAAGAGCG CCTCCACAGA CAGCTCAGTG GAATAGTGGA GGAGCCACTT 
CACTGCCTTT TTAATGACCT CTTCTTTGTG AAGTTTCCCT TATCCTTCAA AGTTTCACGC 
ATGGATGACC TGGCCAGACA ACAGAAGATT AAAGCATGTT TTTTTGAAGT GCTTGAGAAG 
GCAGTAAGGG AAACACCACT GATTTTCCTC ATTGACGAGG CCCAGTATAT GGATGCAGCT 
TCCTGGGAAT TTTTGGGAAC ATTGCTCTCC GGTGTGCCTA TCATCATGGT GATGACGTTG 
ACCACTACCT CCACCCTGTG TGGCCAGGCC CAGCACTTCC TGCAGAGCCC TCCAGCTGTC 
CTGGTGCCCA TTTCAAAGTT GGCAGCGACC TCACTGTTGA GAATGGCATG TTGGGAACTG 
GGGGTGGTGA GCATCCCCCA AGAGCTGCAG ACCTACCTTC TCCGCAGGTG CTTTGGAAAC 
CCCCTTTATT GTGAGGTCTT ATGCCAGGAT CTTCTCTCTA AAAACGTATT GTGCTTTCAT 
GACCTCCAAA AGGAGGAAGT GGAGAACAGC AAGTGGGAGA CCCTCTCAGC CAATGCCATG 
AAATCCACCA TGTACAGTGT TTCTCCTGCC AGCTCTGAAG ACCAGGAACT TTATGTCTGC 
ACAGTCAAGG ATGATGTGAA CTTGGATACA GTGCTTCTTC CACCGTTATT AAAAGAAATA 
GCAGTGAACC AAATAGATCA ACTGAGCCCA GAGGAACAGT CGCTGGTTAA ATGTGCTGCA 
GTCATTGGTC ACTTCTTCCA CATAGATCTG CTGCAGCACC TCCTGCCTGG CTGGAGTAAG 
AATAAGCTAC TTGAGGTACT GAAGGATCTT GTGGATATGC ACGTGCTCCA CTGGTGCAAT 
AAGGGCCAAG AGCTTCCTGC TGAGCCAATA TTAGTGCCTT CCTCTGTCGA GACTGAGCAT 
ACCAAAGAGA CGACAAAGTC AGATGCTGAC TCTCCTCTAA AGCTACAAGA GGAACTATCC 
CTACCTCAGA TGGAGGTGCT TGAATTTGGA GTGCCTCTTT TGCGGGAAGG GGCTTGGGAG 
CTGTGGCCCA AGGCACAGCA AACAGCCCTA CACCTTGAAT GTGCCTGCTT TCTTCGAGAT 
CTGGCCTGCC GCTGTGGGAG CTGTCATGGG GGAGACTTTG TCCCCTTCCA CCGTTTCGCC 
ATCTGTTCCA CTAAGAGCTC CAGCAGGACC TCCCGATTCT GTAGCTACAA AGACACTGGC 
TCGATACTAA CACAAGTGAT CACAGACAGA TTGCAGCTGC CTTCTCCCCA AGACACTTTT 
CAGTTCCAGA CAGAATCATC CAGAAGTCAG GAGGCCTTCA CAAGGGAATA CTGCAGAACA 
GAGGAAGAGT TCCTGGATAA AGTGAACAGG AAACTGGCTC AGACCAACCC CAAGAAAGGC 
ATGTTGACCA CGAAGCCCTG CCACTGTAGG GAAGTCCTGA AGTTAGTGCT CTCGCCCCTC 
ACCCAGCACT GCCTGGTCAT TGGAGAAACC ACGTGTGCAT TTTATTACCT GCTGGAATCT 
GCCGCTGCCT CCTTGGATCT GTCAGATAAT TACATGGCCT TCTTTTATTT GAGGAAGGCA 
AGCAGTCTTC TGGGCCAACC CTCCGCATTC TCGTTTTTTA AAAAGCACAA GGTGAAGATC 
TGTCAGTTTG AAGAAGCCAC TTTCTGTCGT CTCCAGTCAG AGGTCTGTTT CAACATGGGA 
CAGATCACCC TGGCCAAAAA ACTGGCTAGG CGAGCCCTTC GTCTGCTGAA AAGGAGTTTT 
CCCTGGACCT GGCTTGGTGT CATTTTCCAA ACATTTCTAG AAAAATGCTG GTATTCCTGT 
TCCCTGAGTC AACCTCGAAA CAACCCTAGT GAGAAGAAGA AGAAGCTGGC AGTCCTGCAG 
CAGCAGATGC ACTGTCTCTC CCTGCTCCGG CAGCTCTACA GCCTGGAGGC CACAGCCAGC 
AGCCTCAGGT TTGCCTGCCT GGCCACTCTT ATGCAGAAGA ATTCAGTCAA GGAGTTTCCT 
GGTGAAGCCC AGGTTGTCTC TACCTATGTG GCCCTCTCCC AGTTCTCCCA GAACGTGGGT 
GACAAGGAGA AGTGGCTGTA CCATGAGCAG CTGGCCATTC AGAAAAGCAG CCTGTGCTGG 
TTCTCCAGGG AGGGACTGTT GGCCACAGCC CAGCTCATGC AGGCCTTGGC CTACACCAAA 
CTCTGCCTTG GGCATCTCGA CTTCTCCATC AAGCTGGGTT TTCAAGCTCA TGAGATATGC 
AGACACCTCA AGAAACCAGC TCTGGAGAAC CTGGTTCTCT CAGTTCTCTT CAGATCTGCA 
TTTCTGAAGA AGAAGTTTGA TCTGTGTGTC CACGTGCTGG AGAGTCAGTG GGTGCTCATT 
TCCCAGAGTC ATGATGTCCT TGGCCGGGCC TTCTTCTATT CTGCTTGCTT AGATCTGCTG 
CTCTACGGAA AAGGATGGCT GTGTCGGCCC TTTGGGGAAT GTCTGCATTT CATTCAACGC 
TATGAGCACA GCTGCATTCT AAATTCTCAG AGCAGTGTCA TGCTGGGGGT CCACTCCTCT 
CTGGCCATGT GGTTTGCCCA GGACTTACAG TGGAATCAGT TTCAGTACCA CTTCTCCAAG 
GCTCGCCAGT TGGTGAAAAG AACCAATGCC TCACTATTTG GTTCCCATGG CTTTGTCCGA 
TTCCTAGAGT GCCAGGTGCT AATTTTACAG AAAATGCCAG AAGATGTCTT CCTGCAAACT 
CTCGCGGAGA CTCGCCGCCA AACCCTCACG TACTTTAAGG AGTTTTTCTC TCGATGTGTG 
ACCTGCCCTG TCTATCACCA GTGGGTCTCT GAGCTTAAAG CCTTTGTAAT GAGAAATGGA 
AAGATGTCCT TGACCAACAT CATCAGACCT TTTGACACCT GCCTGACCAG GATTTGGATA 
GAGGGAGAAT TCCTGGAGGA AAATAACTCC TTTGAACAAA ATTTGGAAAT ACAGAGATTT 
GTCAGAGAGG CTGATGTCAA CAAGAAGGAT AAAGAAGAAA TTCAAGAATG TATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005192979.2
CDS	16..4815
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTDVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMWFAQDLQWNQFQYHFSKARQLVKRTNASLFGSHGFVRFLECQVLILQKMPEDVFLQTLAETRRQTLTYFKEFFSRCVTCPVYHQWVSELKAFVMRNGKMSLTNIIRPFDTCLTRIWIEGEFLEENNSFEQNLEIQRFVREADVNKKDKEEIQECIC

Target ORF information:

RefSeq Version	XM_005192922.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005192922.3

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGATGTGCT CTGTTTTGGA 
GGAGATATCC TAAATATCAC AGGGAATGTG CTCCTAGCAC TCTGGACAGT GGAACAAAAT 
CAACTGAGTG ACATCATTAC TCTGGTGGCA AAATGCAGTC TGGAGATACA GGAGAAATTT 
GGGATCTACC ATACCAGAGA AGGCCAGGAC CTGCAGCTAA AGATAGGTCT ATCTGCAGGT 
CGGATTTCCC AGGTTATTGT TGGAGATGAG CAGCGCCAGT ACCTCTTGGT GATTGGCCGG 
GATGTAGATG GTGTGCAGTT AGCTCAACGG TTGGCTCAAG CAAATGAGAT TGTCCTGTCC 
TGGAACTGCT GGAAGCTCTG TGAGCAGTAC ATGTTTGAAG TGGAAATCAT GCGGGAGGAT 
GAAGCTGTGA AGTTAAGAGA TATGCGGATC ATTGACCCAT TTGATTTTGA TGAGCATTTT 
TACAAGTGCC TCGATTATCT GCCACATTAC CGGACAAGTG CTGACATTCT AAGAGCCGCC 
CTGGAGTTGG ATCCAGATTC TGCACTTGAG CAAACCCTGC GGAAGCACAT AATGAAAAAC 
CTTTTGAAGA AGATTGATGA AGGCCAGCCT ATGGAGTATA TATCCGAATT CCGCACTGTG 
ACTATGGTTT TGGTCAGCCT CGAGTTCCAC AAGACAGCAT GGGTGTTGCA TTTGTGCCAT 
CTTATCCAGG AGGCTGCGTT ATACATCTCC ACAGTGATAG AGACAGGGGG TGGCCAGCTG 
AGCCGGATCT TTATGTTTGA GACAGGCTGC ATGTTCCTCT GTGTTTTCGG CCTACCTGGG 
GATAAGAAGC CAGACGAGTG TGCACATGCC CTGGAGAGCT CCTTCAGCAT CTTCAGCTTC 
TGCTGGGAGA ATCTTGCTGA GACCAAGCTT GTTTCCATCA GTATCACCAA TGGACCAGTA 
TTCTGTGGCA TGGTTGGGGC AGTTTCAAGA CACGAATATA CAGTTATTGG CCCAAAAGTA 
AGCCTCGTGG CAAGAATGAT AACTGCTTAT CCAGGTTTGG TGTCCTGTGA TGAGGTAACA 
TATCTGAGAT CTATGCTACC TGCTTACAAC TTCAAGAAAC TCCCAGAGAA AATGATGAAA 
AATATCTCTA ACCCAGGGAA GATGTATGAA TACGTTGGCC ATAGAAGATG TATAATGTTT 
GGGAAGAGAC ATTTGGCCAC AGAGAGAAAT AAAAATCACT CTTTGCTAGA CCGGGAGAAA 
GAACTGGAAG CTTTCCGAAT GGCACAGCAG GAATGTTTGC ACCAGAAGAA GGGACAGGCA 
GTTCTATATG AAGGTGGAAA AGGCAGTGGA AAAAGTCAGC TCTTGGCTGA AATAAACTTC 
CTGGCCCAGA AGGAAGGGCA CAGGGTGTTG CCGTTGAAGC TGAAGAAAGC GGATTCCAAG 
CAGTGCTTCT ACGCCATCCA GACCCTCATG GCCATCTTCT TCGAGATTGA TACATGCCCA 
GCCCATTACC GGCAAGAGCG CCTCCACAGA CAGCTCAGTG GAATAGTGGA GGAGCCACTT 
CACTGCCTTT TTAATGACCT CTTCTTTGTG AAGTTTCCCT TATCCTTCAA AGTTTCACGC 
ATGGATGACC TGGCCAGACA ACAGAAGATT AAAGCATGTT TTTTTGAAGT GCTTGAGAAG 
GCAGTAAGGG AAACACCACT GATTTTCCTC ATTGACGAGG CCCAGTATAT GGATGCAGCT 
TCCTGGGAAT TTTTGGGAAC ATTGCTCTCC GGTGTGCCTA TCATCATGGT GATGACGTTG 
ACCACTACCT CCACCCTGTG TGGCCAGGCC CAGCACTTCC TGCAGAGCCC TCCAGCTGTC 
CTGGTGCCCA TTTCAAAGTT GGCAGCGACC TCACTGTTGA GAATGGCATG TTGGGAACTG 
GGGGTGGTGA GCATCCCCCA AGAGCTGCAG ACCTACCTTC TCCGCAGGTG CTTTGGAAAC 
CCCCTTTATT GTGAGGTCTT ATGCCAGGAT CTTCTCTCTA AAAACGTATT GTGCTTTCAT 
GACCTCCAAA AGGAGGAAGT GGAGAACAGC AAGTGGGAGA CCCTCTCAGC CAATGCCATG 
AAATCCACCA TGTACAGTGT TTCTCCTGCC AGCTCTGAAG ACCAGGAACT TTATGTCTGC 
ACAGTCAAGG ATGATGTGAA CTTGGATACA GTGCTTCTTC CACCGTTATT AAAAGAAATA 
GCAGTGAACC AAATAGATCA ACTGAGCCCA GAGGAACAGT CGCTGGTTAA ATGTGCTGCA 
GTCATTGGTC ACTTCTTCCA CATAGATCTG CTGCAGCACC TCCTGCCTGG CTGGAGTAAG 
AATAAGCTAC TTGAGGTACT GAAGGATCTT GTGGATATGC ACGTGCTCCA CTGGTGCAAT 
AAGGGCCAAG AGCTTCCTGC TGAGCCAATA TTAGTGCCTT CCTCTGTCGA GACTGAGCAT 
ACCAAAGAGA CGACAAAGTC AGATGCTGAC TCTCCTCTAA AGCTACAAGA GGAACTATCC 
CTACCTCAGA TGGAGGTGCT TGAATTTGGA GTGCCTCTTT TGCGGGAAGG GGCTTGGGAG 
CTGTGGCCCA AGGCACAGCA AACAGCCCTA CACCTTGAAT GTGCCTGCTT TCTTCGAGAT 
CTGGCCTGCC GCTGTGGGAG CTGTCATGGG GGAGACTTTG TCCCCTTCCA CCGTTTCGCC 
ATCTGTTCCA CTAAGAGCTC CAGCAGGACC TCCCGATTCT GTAGCTACAA AGACACTGGC 
TCGATACTAA CACAAGTGAT CACAGACAGA TTGCAGCTGC CTTCTCCCCA AGACACTTTT 
CAGTTCCAGA CAGAATCATC CAGAAGTCAG GAGGCCTTCA CAAGGGAATA CTGCAGAACA 
GAGGAAGAGT TCCTGGATAA AGTGAACAGG AAACTGGCTC AGACCAACCC CAAGAAAGGC 
ATGTTGACCA CGAAGCCCTG CCACTGTAGG GAAGTCCTGA AGTTAGTGCT CTCGCCCCTC 
ACCCAGCACT GCCTGGTCAT TGGAGAAACC ACGTGTGCAT TTTATTACCT GCTGGAATCT 
GCCGCTGCCT CCTTGGATCT GTCAGATAAT TACATGGCCT TCTTTTATTT GAGGAAGGCA 
AGCAGTCTTC TGGGCCAACC CTCCGCATTC TCGTTTTTTA AAAAGCACAA GGTGAAGATC 
TGTCAGTTTG AAGAAGCCAC TTTCTGTCGT CTCCAGTCAG AGGTCTGTTT CAACATGGGA 
CAGATCACCC TGGCCAAAAA ACTGGCTAGG CGAGCCCTTC GTCTGCTGAA AAGGAGTTTT 
CCCTGGACCT GGCTTGGTGT CATTTTCCAA ACATTTCTAG AAAAATGCTG GTATTCCTGT 
TCCCTGAGTC AACCTCGAAA CAACCCTAGT GAGAAGAAGA AGAAGCTGGC AGTCCTGCAG 
CAGCAGATGC ACTGTCTCTC CCTGCTCCGG CAGCTCTACA GCCTGGAGGC CACAGCCAGC 
AGCCTCAGGT TTGCCTGCCT GGCCACTCTT ATGCAGAAGA ATTCAGTCAA GGAGTTTCCT 
GGTGAAGCCC AGGTTGTCTC TACCTATGTG GCCCTCTCCC AGTTCTCCCA GAACGTGGGT 
GACAAGGAGA AGTGGCTGTA CCATGAGCAG CTGGCCATTC AGAAAAGCAG CCTGTGCTGG 
TTCTCCAGGG AGGGACTGTT GGCCACAGCC CAGCTCATGC AGGCCTTGGC CTACACCAAA 
CTCTGCCTTG GGCATCTCGA CTTCTCCATC AAGCTGGGTT TTCAAGCTCA TGAGATATGC 
AGACACCTCA AGAAACCAGC TCTGGAGAAC CTGGTTCTCT CAGTTCTCTT CAGATCTGCA 
TTTCTGAAGA AGAAGTTTGA TCTGTGTGTC CACGTGCTGG AGAGTCAGTG GGTGCTCATT 
TCCCAGAGTC ATGATGTCCT TGGCCGGGCC TTCTTCTATT CTGCTTGCTT AGATCTGCTG 
CTCTACGGAA AAGGATGGCT GTGTCGGCCC TTTGGGGAAT GTCTGCATTT CATTCAACGC 
TATGAGCACA GCTGCATTCT AAATTCTCAG AGCAGTGTCA TGCTGGGGGT CCACTCCTCT 
CTGGCCATGT GGTTTGCCCA GGACTTACAG TGGAATCAGT TTCAGTACCA CTTCTCCAAG 
GCTCGCCAGT TGGTGAAAAG AACCAATGCC TCACTATTTG GTTCCCATGG CTTTGTCCGA 
TTCCTAGAGT GCCAGGTGCT AATTTTACAG AAAATGCCAG AAGATGTCTT CCTGCAAACT 
CTCGCGGAGA CTCGCCGCCA AACCCTCACG TACTTTAAGG AGTTTTTCTC TCGATGTGTG 
ACCTGCCCTG TCTATCACCA GTGGGTCTCT GAGCTTAAAG CCTTTGTAAT GAGAAATGGA 
AAGATGTCCT TGACCAACAT CATCAGACCT TTTGACACCT GCCTGACCAG GATTTGGATA 
GAGGGAGAAT TCCTGGAGGA AAATAACTCC TTTGAACAAA ATTTGGAAAT ACAGAGATTT 
GTCAGAGAGG CTGATGTCAA CAAGAAGGAT AAAGAAGAAA TTCAAGAATG TATCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005223527.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223584.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.

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CloneID	OBa52051
Clone ID Related Accession (Same CDS sequence)	XM_005223527.3 , XM_024983977.1 , XM_005223527.4 , XM_005192926.3
Accession Version	XM_005223527.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223527.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223584.2
CDS	16..4455
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMW

Target ORF information:

RefSeq Version	XM_005223527.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223527.3

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_024983977.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839745.1 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X6, mRNA.

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CloneID	OBa52051
Clone ID Related Accession (Same CDS sequence)	XM_005223527.3 , XM_024983977.1 , XM_005223527.4 , XM_005192926.3
Accession Version	XM_024983977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839745.1
CDS	711..5150
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMW

Target ORF information:

RefSeq Version	XM_024983977.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983977.1

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005223527.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223584.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa52051
Clone ID Related Accession (Same CDS sequence)	XM_005223527.3 , XM_024983977.1 , XM_005223527.4 , XM_005192926.3
Accession Version	XM_005223527.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223527.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223584.2
CDS	711..5150
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMW

Target ORF information:

RefSeq Version	XM_005223527.4
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223527.4

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC531747 ( XM_005192926.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005192983.2 Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa52051
Clone ID Related Accession (Same CDS sequence)	XM_005223527.3 , XM_024983977.1 , XM_005223527.4 , XM_005192926.3
Accession Version	XM_005192926.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate cyclase type 10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192926.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005192983.2
CDS	16..4455
Translation	MAPSESALIGWWEGPVRAVVEDWLLRGGTPVSGNSTGKPAAAMATAKRLAAPPLWPVEARLAALLPDLLVFRNPRGSEPELATAQGVLLGVHVTGFTALMDRFSLSCKLEHDMDRLAHIFSYYISDIVEHVLCFGGDILNITGNVLLALWTVEQNQLSDIITLVAKCSLEIQEKFGIYHTREGQDLQLKIGLSAGRISQVIVGDEQRQYLLVIGRDVDGVQLAQRLAQANEIVLSWNCWKLCEQYMFEVEIMREDEAVKLRDMRIIDPFDFDEHFYKCLDYLPHYRTSADILRAALELDPDSALEQTLRKHIMKNLLKKIDEGQPMEYISEFRTVTMVLVSLEFHKTAWVLHLCHLIQEAALYISTVIETGGGQLSRIFMFETGCMFLCVFGLPGDKKPDECAHALESSFSIFSFCWENLAETKLVSISITNGPVFCGMVGAVSRHEYTVIGPKVSLVARMITAYPGLVSCDEVTYLRSMLPAYNFKKLPEKMMKNISNPGKMYEYVGHRRCIMFGKRHLATERNKNHSLLDREKELEAFRMAQQECLHQKKGQAVLYEGGKGSGKSQLLAEINFLAQKEGHRVLPLKLKKADSKQCFYAIQTLMAIFFEIDTCPAHYRQERLHRQLSGIVEEPLHCLFNDLFFVKFPLSFKVSRMDDLARQQKIKACFFEVLEKAVRETPLIFLIDEAQYMDAASWEFLGTLLSGVPIIMVMTLTTTSTLCGQAQHFLQSPPAVLVPISKLAATSLLRMACWELGVVSIPQELQTYLLRRCFGNPLYCEVLCQDLLSKNVLCFHDLQKEEVENSKWETLSANAMKSTMYSVSPASSEDQELYVCTVKDDVNLDTVLLPPLLKEIAVNQIDQLSPEEQSLVKCAAVIGHFFHIDLLQHLLPGWSKNKLLEVLKDLVDMHVLHWCNKGQELPAEPILVPSSVETEHTKETTKSDADSPLKLQEELSLPQMEVLEFGVPLLREGAWELWPKAQQTALHLECACFLRDLACRCGSCHGGDFVPFHRFAICSTKSSSRTSRFCSYKDTGSILTQVITDRLQLPSPQDTFQFQTESSRSQEAFTREYCRTEEEFLDKVNRKLAQTNPKKGMLTTKPCHCREVLKLVLSPLTQHCLVIGETTCAFYYLLESAAASLDLSDNYMAFFYLRKASSLLGQPSAFSFFKKHKVKICQFEEATFCRLQSEVCFNMGQITLAKKLARRALRLLKRSFPWTWLGVIFQTFLEKCWYSCSLSQPRNNPSEKKKKLAVLQQQMHCLSLLRQLYSLEATASSLRFACLATLMQKNSVKEFPGEAQVVSTYVALSQFSQNVGDKEKWLYHEQLAIQKSSLCWFSREGLLATAQLMQALAYTKLCLGHLDFSIKLGFQAHEICRHLKKPALENLVLSVLFRSAFLKKKFDLCVHVLESQWVLISQSHDVLGRAFFYSACLDLLLYGKGWLCRPFGECLHFIQRYEHSCILNSQSSVMLGVHSSLAMW

Target ORF information:

RefSeq Version	XM_005192926.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate cyclase type 10 (LOC531747), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005192926.3

ORF Insert Sequence:

ATGGCCCCAA GTGAGTCCGC ACTGATTGGT TGGTGGGAGG GACCAGTCAG GGCCGTCGTT 
GAGGATTGGC TACTACGCGG AGGGACCCCC GTTTCCGGTA ACTCGACGGG CAAGCCCGCC 
GCCGCCATGG CAACCGCCAA GCGGCTGGCG GCGCCGCCTC TATGGCCAGT AGAGGCCAGG 
TTGGCGGCGC TGCTTCCCGA CCTACTGGTC TTCAGGAATC CCAGGGGATC TGAACCCGAG 
CTCGCCACGG CCCAGGGCGT CCTCCTTGGC GTCCACGTGA CGGGGTTTAC AGCATTAATG 
GACAGGTTCA GCCTGAGCTG CAAGTTGGAA CATGACATGG ACAGACTGGC CCACATCTTC 
AGTTATTACA TTAGTGACAT CGTGGAGCAT GTGCTCTGTT TTGGAGGAGA TATCCTAAAT 
ATCACAGGGA ATGTGCTCCT AGCACTCTGG ACAGTGGAAC AAAATCAACT GAGTGACATC 
ATTACTCTGG TGGCAAAATG CAGTCTGGAG ATACAGGAGA AATTTGGGAT CTACCATACC 
AGAGAAGGCC AGGACCTGCA GCTAAAGATA GGTCTATCTG CAGGTCGGAT TTCCCAGGTT 
ATTGTTGGAG ATGAGCAGCG CCAGTACCTC TTGGTGATTG GCCGGGATGT AGATGGTGTG 
CAGTTAGCTC AACGGTTGGC TCAAGCAAAT GAGATTGTCC TGTCCTGGAA CTGCTGGAAG 
CTCTGTGAGC AGTACATGTT TGAAGTGGAA ATCATGCGGG AGGATGAAGC TGTGAAGTTA 
AGAGATATGC GGATCATTGA CCCATTTGAT TTTGATGAGC ATTTTTACAA GTGCCTCGAT 
TATCTGCCAC ATTACCGGAC AAGTGCTGAC ATTCTAAGAG CCGCCCTGGA GTTGGATCCA 
GATTCTGCAC TTGAGCAAAC CCTGCGGAAG CACATAATGA AAAACCTTTT GAAGAAGATT 
GATGAAGGCC AGCCTATGGA GTATATATCC GAATTCCGCA CTGTGACTAT GGTTTTGGTC 
AGCCTCGAGT TCCACAAGAC AGCATGGGTG TTGCATTTGT GCCATCTTAT CCAGGAGGCT 
GCGTTATACA TCTCCACAGT GATAGAGACA GGGGGTGGCC AGCTGAGCCG GATCTTTATG 
TTTGAGACAG GCTGCATGTT CCTCTGTGTT TTCGGCCTAC CTGGGGATAA GAAGCCAGAC 
GAGTGTGCAC ATGCCCTGGA GAGCTCCTTC AGCATCTTCA GCTTCTGCTG GGAGAATCTT 
GCTGAGACCA AGCTTGTTTC CATCAGTATC ACCAATGGAC CAGTATTCTG TGGCATGGTT 
GGGGCAGTTT CAAGACACGA ATATACAGTT ATTGGCCCAA AAGTAAGCCT CGTGGCAAGA 
ATGATAACTG CTTATCCAGG TTTGGTGTCC TGTGATGAGG TAACATATCT GAGATCTATG 
CTACCTGCTT ACAACTTCAA GAAACTCCCA GAGAAAATGA TGAAAAATAT CTCTAACCCA 
GGGAAGATGT ATGAATACGT TGGCCATAGA AGATGTATAA TGTTTGGGAA GAGACATTTG 
GCCACAGAGA GAAATAAAAA TCACTCTTTG CTAGACCGGG AGAAAGAACT GGAAGCTTTC 
CGAATGGCAC AGCAGGAATG TTTGCACCAG AAGAAGGGAC AGGCAGTTCT ATATGAAGGT 
GGAAAAGGCA GTGGAAAAAG TCAGCTCTTG GCTGAAATAA ACTTCCTGGC CCAGAAGGAA 
GGGCACAGGG TGTTGCCGTT GAAGCTGAAG AAAGCGGATT CCAAGCAGTG CTTCTACGCC 
ATCCAGACCC TCATGGCCAT CTTCTTCGAG ATTGATACAT GCCCAGCCCA TTACCGGCAA 
GAGCGCCTCC ACAGACAGCT CAGTGGAATA GTGGAGGAGC CACTTCACTG CCTTTTTAAT 
GACCTCTTCT TTGTGAAGTT TCCCTTATCC TTCAAAGTTT CACGCATGGA TGACCTGGCC 
AGACAACAGA AGATTAAAGC ATGTTTTTTT GAAGTGCTTG AGAAGGCAGT AAGGGAAACA 
CCACTGATTT TCCTCATTGA CGAGGCCCAG TATATGGATG CAGCTTCCTG GGAATTTTTG 
GGAACATTGC TCTCCGGTGT GCCTATCATC ATGGTGATGA CGTTGACCAC TACCTCCACC 
CTGTGTGGCC AGGCCCAGCA CTTCCTGCAG AGCCCTCCAG CTGTCCTGGT GCCCATTTCA 
AAGTTGGCAG CGACCTCACT GTTGAGAATG GCATGTTGGG AACTGGGGGT GGTGAGCATC 
CCCCAAGAGC TGCAGACCTA CCTTCTCCGC AGGTGCTTTG GAAACCCCCT TTATTGTGAG 
GTCTTATGCC AGGATCTTCT CTCTAAAAAC GTATTGTGCT TTCATGACCT CCAAAAGGAG 
GAAGTGGAGA ACAGCAAGTG GGAGACCCTC TCAGCCAATG CCATGAAATC CACCATGTAC 
AGTGTTTCTC CTGCCAGCTC TGAAGACCAG GAACTTTATG TCTGCACAGT CAAGGATGAT 
GTGAACTTGG ATACAGTGCT TCTTCCACCG TTATTAAAAG AAATAGCAGT GAACCAAATA 
GATCAACTGA GCCCAGAGGA ACAGTCGCTG GTTAAATGTG CTGCAGTCAT TGGTCACTTC 
TTCCACATAG ATCTGCTGCA GCACCTCCTG CCTGGCTGGA GTAAGAATAA GCTACTTGAG 
GTACTGAAGG ATCTTGTGGA TATGCACGTG CTCCACTGGT GCAATAAGGG CCAAGAGCTT 
CCTGCTGAGC CAATATTAGT GCCTTCCTCT GTCGAGACTG AGCATACCAA AGAGACGACA 
AAGTCAGATG CTGACTCTCC TCTAAAGCTA CAAGAGGAAC TATCCCTACC TCAGATGGAG 
GTGCTTGAAT TTGGAGTGCC TCTTTTGCGG GAAGGGGCTT GGGAGCTGTG GCCCAAGGCA 
CAGCAAACAG CCCTACACCT TGAATGTGCC TGCTTTCTTC GAGATCTGGC CTGCCGCTGT 
GGGAGCTGTC ATGGGGGAGA CTTTGTCCCC TTCCACCGTT TCGCCATCTG TTCCACTAAG 
AGCTCCAGCA GGACCTCCCG ATTCTGTAGC TACAAAGACA CTGGCTCGAT ACTAACACAA 
GTGATCACAG ACAGATTGCA GCTGCCTTCT CCCCAAGACA CTTTTCAGTT CCAGACAGAA 
TCATCCAGAA GTCAGGAGGC CTTCACAAGG GAATACTGCA GAACAGAGGA AGAGTTCCTG 
GATAAAGTGA ACAGGAAACT GGCTCAGACC AACCCCAAGA AAGGCATGTT GACCACGAAG 
CCCTGCCACT GTAGGGAAGT CCTGAAGTTA GTGCTCTCGC CCCTCACCCA GCACTGCCTG 
GTCATTGGAG AAACCACGTG TGCATTTTAT TACCTGCTGG AATCTGCCGC TGCCTCCTTG 
GATCTGTCAG ATAATTACAT GGCCTTCTTT TATTTGAGGA AGGCAAGCAG TCTTCTGGGC 
CAACCCTCCG CATTCTCGTT TTTTAAAAAG CACAAGGTGA AGATCTGTCA GTTTGAAGAA 
GCCACTTTCT GTCGTCTCCA GTCAGAGGTC TGTTTCAACA TGGGACAGAT CACCCTGGCC 
AAAAAACTGG CTAGGCGAGC CCTTCGTCTG CTGAAAAGGA GTTTTCCCTG GACCTGGCTT 
GGTGTCATTT TCCAAACATT TCTAGAAAAA TGCTGGTATT CCTGTTCCCT GAGTCAACCT 
CGAAACAACC CTAGTGAGAA GAAGAAGAAG CTGGCAGTCC TGCAGCAGCA GATGCACTGT 
CTCTCCCTGC TCCGGCAGCT CTACAGCCTG GAGGCCACAG CCAGCAGCCT CAGGTTTGCC 
TGCCTGGCCA CTCTTATGCA GAAGAATTCA GTCAAGGAGT TTCCTGGTGA AGCCCAGGTT 
GTCTCTACCT ATGTGGCCCT CTCCCAGTTC TCCCAGAACG TGGGTGACAA GGAGAAGTGG 
CTGTACCATG AGCAGCTGGC CATTCAGAAA AGCAGCCTGT GCTGGTTCTC CAGGGAGGGA 
CTGTTGGCCA CAGCCCAGCT CATGCAGGCC TTGGCCTACA CCAAACTCTG CCTTGGGCAT 
CTCGACTTCT CCATCAAGCT GGGTTTTCAA GCTCATGAGA TATGCAGACA CCTCAAGAAA 
CCAGCTCTGG AGAACCTGGT TCTCTCAGTT CTCTTCAGAT CTGCATTTCT GAAGAAGAAG 
TTTGATCTGT GTGTCCACGT GCTGGAGAGT CAGTGGGTGC TCATTTCCCA GAGTCATGAT 
GTCCTTGGCC GGGCCTTCTT CTATTCTGCT TGCTTAGATC TGCTGCTCTA CGGAAAAGGA 
TGGCTGTGTC GGCCCTTTGG GGAATGTCTG CATTTCATTC AACGCTATGA GCACAGCTGC 
ATTCTAAATT CTCAGAGCAG TGTCATGCTG GGGGTCCACT CCTCTCTGGC CATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bos taurus (cattle)	LOC531747	XP_005223578.1
	LOC719662	XP_001114224.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC531747 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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