SEMA3F cDNA ORF clone, Bos taurus(cattle)

The following SEMA3F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA3F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa229319 XM_024983273.1
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229319 XM_010817850.3
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229319 XM_024983272.1
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229319 XM_002697033.6
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229320 XM_005223007.4
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa11067 XM_609627.7
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa11067 XM_010825144.2
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa11067 XM_002697033.5
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa11067 XM_010817850.2
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa11068 XM_005223007.3
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa11068 XM_005196475.3
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa51943 XM_010825145.2
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OBa51943 XM_010817851.2
Latest version!
Bos taurus semaphorin 3F (SEMA3F), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa229319
    Clone ID Related Accession (Same CDS sequence) XM_024983272.1 , XM_024983273.1 , XM_002697033.6 , XM_010817850.3
    Accession Version XM_024983272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGATGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCTGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCTGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC GTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839040.1
    CDS397..2775
    Translation

    Target ORF information:

    RefSeq Version XM_024983272.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGATGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCTGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCTGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC GTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229319
    Clone ID Related Accession (Same CDS sequence) XM_024983272.1 , XM_024983273.1 , XM_002697033.6 , XM_010817850.3
    Accession Version XM_024983273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGATGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCTGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCTGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC GTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839041.1
    CDS243..2621
    Translation

    Target ORF information:

    RefSeq Version XM_024983273.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGATGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCTGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCTGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC GTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229319
    Clone ID Related Accession (Same CDS sequence) XM_024983272.1 , XM_024983273.1 , XM_002697033.6 , XM_010817850.3
    Accession Version XM_002697033.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697033.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGATGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCTGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCTGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC GTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697079.2
    CDS264..2642
    Translation

    Target ORF information:

    RefSeq Version XM_002697033.6
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002697033.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGATGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCTGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCTGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC GTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229319
    Clone ID Related Accession (Same CDS sequence) XM_024983272.1 , XM_024983273.1 , XM_002697033.6 , XM_010817850.3
    Accession Version XM_010817850.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGATGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCTGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCTGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC GTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816152.2
    CDS254..2632
    Translation

    Target ORF information:

    RefSeq Version XM_010817850.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817850.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGATGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCTGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCTGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC GTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229320
    Clone ID Related Accession (Same CDS sequence) XM_005223007.4
    Accession Version XM_005223007.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223007.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGATT ACGTCTTCTA CCTGGAGCCT
    GAGAAGCTCG AGTCAGGGAA GGGCAAGTGT CCCTACGACC CCAAGCTGGA CACAGCCTCA
    GCCCTCATCA ATGAGGAGCT CTATGCAGGC GTGTACATCG ACTTCATGGG CACGGACGCA
    GCCATCTTCC GCACCCTTGG CAAGCAGACG GCCATGCGCA CGGATCAGTA CAACTCTCGG
    TGGCTCAATG ACCCTTCTTT CATCCACGCC GAGCTCATCC CTGACAGTGC CGAGCGCAAC
    GATGACAAGC TCTACTTCTT CTTCCGTGAG CGGTCCATAG AGGCGCCGCA GAGCCCTGCC
    GTGTACGCCC GCATTGGGCG GATCTGCCTG AATGATGACG GCGGCCACTG CTGCCTGGTC
    AACAAGTGGA GCACATTCCT GAAGGCAAGG CTGGTCTGCT CTGTGCCAGG CGAGGATGGC
    ATCGAGACCC ACTTCGACGA GCTCCAGGAT GTGTTTGTCC AGCAGACCCA GGATGTGAGG
    AACCCAGTCA TCTACGCTGT CTTCACCTCC TCGGGCTCTG TGTTCCGAGG CTCTGCCGTG
    TGTGTCTACT CCATGGCTGA CATCCGCATG GTCTTCAATG GGCCCTTTGC CCACAAGGAG
    GGCCCCAACT ACCAGTGGAT GCCCTTCTCA GGAAAGATGC CCTACCCACG GCCTGGCACG
    TGCCCTGGCG GAACCTTCAC GCCGTCCATG AAGTCCACCA AAGACTACCC TGATGAAGTC
    ATCAACTTCA TGCGCAGCCA CCCGCTCATG TACCAGGCCA TCTACCCTCT GCAGCGGCGG
    CCCCTGGTGG TCCGCACAGG CGCGCCCTAC CGTCTCACCA CTGTGGCCGT GGACCAGGTG
    GACGCAGCCG ACGGGCGCTA TGAGGTGCTT TTCCTGGGCA CAGACCGCGG GACAGTGCAG
    AAGGTCATCG TGCTGCCTAA GGACGACCAG GAGATGGAGG AGCTCATGCT AGAGGAGGTC
    GAGGTCTTCA AGGACCCAGC ACCTGTTAAG ACCATGACCA TCTCTTCCAA GAGGCAACAA
    CTGTACGTGG CCTCAGCCGT GGGCGTCACA CAGCTGAGCC TGCACCGCTG TCAGGCATAT
    GGGGCCGCCT GTGCCGACTG CTGCCTCGCC CGGGACCCCT ACTGCGCCTG GGATGGCCAA
    GCCTGTTCCC GCTACACAGC GTCCTCTAAG AGGCGGAGCC GCCGGCAGGA TGTCCGGCAC
    GGGAACCCCA TCCGGCAGTG CCGTGGGTTC AATTCCAATG CCAATAAGAA CGCTGTGGAG
    TCTGTGCAGT ACGGTGTGGC AGGGAGCACA GCCTTCCTTG AGTGCCAGCC CCGCTCGCCC
    CAGGCTACCG TGAAGTGGCT GTTCCAGCGA GATCCCAGTG ACCGGCGCCG CGAGGTCCAC
    GCGGACGACC GCTTCCTGCG CACTGAACAA GGCTTGCTGC TCCGCGCCCT GCAGTCTGGT
    GATAGCGGCC TCTACTCCTG TACAGCCACC GAGAACAACT TCAAGCACAT CATCACACGG
    GTGCAGCTGC ATGTCCTGGG CCAGGAAGAC ATCCGTACGG CCCTCTTCCC ACCGCCAGCT
    GCAAGCATTC CACCGCCAGC TGCGAGCATT CCACCGCCCC CGGGCACCGG CCCCCCAAAG
    CCCCGTTACC AAGAGCTGGC CCAGCTGCTG GCCCAGCCAG AAGTGGGCCT CATCCACCAG
    TACTGCCAGG GCTACTGGCG CCACGTGCCC CCCAGTCCCA GGGAGGCCCC AGGGGCACCC
    AGGCCTCGTA AACCCCAGGA CCAGAAAAAA CCCCGGAACC GCCGCCACCA CCCACCGGAC
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223064.2
    CDS264..2549
    Translation

    Target ORF information:

    RefSeq Version XM_005223007.4
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223007.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGATT ACGTCTTCTA CCTGGAGCCT
    GAGAAGCTCG AGTCAGGGAA GGGCAAGTGT CCCTACGACC CCAAGCTGGA CACAGCCTCA
    GCCCTCATCA ATGAGGAGCT CTATGCAGGC GTGTACATCG ACTTCATGGG CACGGACGCA
    GCCATCTTCC GCACCCTTGG CAAGCAGACG GCCATGCGCA CGGATCAGTA CAACTCTCGG
    TGGCTCAATG ACCCTTCTTT CATCCACGCC GAGCTCATCC CTGACAGTGC CGAGCGCAAC
    GATGACAAGC TCTACTTCTT CTTCCGTGAG CGGTCCATAG AGGCGCCGCA GAGCCCTGCC
    GTGTACGCCC GCATTGGGCG GATCTGCCTG AATGATGACG GCGGCCACTG CTGCCTGGTC
    AACAAGTGGA GCACATTCCT GAAGGCAAGG CTGGTCTGCT CTGTGCCAGG CGAGGATGGC
    ATCGAGACCC ACTTCGACGA GCTCCAGGAT GTGTTTGTCC AGCAGACCCA GGATGTGAGG
    AACCCAGTCA TCTACGCTGT CTTCACCTCC TCGGGCTCTG TGTTCCGAGG CTCTGCCGTG
    TGTGTCTACT CCATGGCTGA CATCCGCATG GTCTTCAATG GGCCCTTTGC CCACAAGGAG
    GGCCCCAACT ACCAGTGGAT GCCCTTCTCA GGAAAGATGC CCTACCCACG GCCTGGCACG
    TGCCCTGGCG GAACCTTCAC GCCGTCCATG AAGTCCACCA AAGACTACCC TGATGAAGTC
    ATCAACTTCA TGCGCAGCCA CCCGCTCATG TACCAGGCCA TCTACCCTCT GCAGCGGCGG
    CCCCTGGTGG TCCGCACAGG CGCGCCCTAC CGTCTCACCA CTGTGGCCGT GGACCAGGTG
    GACGCAGCCG ACGGGCGCTA TGAGGTGCTT TTCCTGGGCA CAGACCGCGG GACAGTGCAG
    AAGGTCATCG TGCTGCCTAA GGACGACCAG GAGATGGAGG AGCTCATGCT AGAGGAGGTC
    GAGGTCTTCA AGGACCCAGC ACCTGTTAAG ACCATGACCA TCTCTTCCAA GAGGCAACAA
    CTGTACGTGG CCTCAGCCGT GGGCGTCACA CAGCTGAGCC TGCACCGCTG TCAGGCATAT
    GGGGCCGCCT GTGCCGACTG CTGCCTCGCC CGGGACCCCT ACTGCGCCTG GGATGGCCAA
    GCCTGTTCCC GCTACACAGC GTCCTCTAAG AGGCGGAGCC GCCGGCAGGA TGTCCGGCAC
    GGGAACCCCA TCCGGCAGTG CCGTGGGTTC AATTCCAATG CCAATAAGAA CGCTGTGGAG
    TCTGTGCAGT ACGGTGTGGC AGGGAGCACA GCCTTCCTTG AGTGCCAGCC CCGCTCGCCC
    CAGGCTACCG TGAAGTGGCT GTTCCAGCGA GATCCCAGTG ACCGGCGCCG CGAGGTCCAC
    GCGGACGACC GCTTCCTGCG CACTGAACAA GGCTTGCTGC TCCGCGCCCT GCAGTCTGGT
    GATAGCGGCC TCTACTCCTG TACAGCCACC GAGAACAACT TCAAGCACAT CATCACACGG
    GTGCAGCTGC ATGTCCTGGG CCAGGAAGAC ATCCGTACGG CCCTCTTCCC ACCGCCAGCT
    GCAAGCATTC CACCGCCAGC TGCGAGCATT CCACCGCCCC CGGGCACCGG CCCCCCAAAG
    CCCCGTTACC AAGAGCTGGC CCAGCTGCTG GCCCAGCCAG AAGTGGGCCT CATCCACCAG
    TACTGCCAGG GCTACTGGCG CCACGTGCCC CCCAGTCCCA GGGAGGCCCC AGGGGCACCC
    AGGCCTCGTA AACCCCAGGA CCAGAAAAAA CCCCGGAACC GCCGCCACCA CCCACCGGAC
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11067
    Clone ID Related Accession (Same CDS sequence) XM_609627.7 , XM_010817850.2 , XM_010825144.2 , XM_002697033.5
    Accession Version XM_609627.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_609627.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGACGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCCGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCCGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC CTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_609627.3
    CDS146..2524
    Translation

    Target ORF information:

    RefSeq Version XM_609627.7
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_609627.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGACGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCCGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCCGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC CTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11067
    Clone ID Related Accession (Same CDS sequence) XM_609627.7 , XM_010817850.2 , XM_010825144.2 , XM_002697033.5
    Accession Version XM_010817850.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGACGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCCGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCCGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC CTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816152.1
    CDS241..2619
    Translation

    Target ORF information:

    RefSeq Version XM_010817850.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGACGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCCGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCCGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC CTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11067
    Clone ID Related Accession (Same CDS sequence) XM_609627.7 , XM_010817850.2 , XM_010825144.2 , XM_002697033.5
    Accession Version XM_010825144.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGACGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCCGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCCGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC CTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010823446.1
    CDS241..2619
    Translation

    Target ORF information:

    RefSeq Version XM_010825144.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010825144.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGACGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCCGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCCGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC CTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11067
    Clone ID Related Accession (Same CDS sequence) XM_609627.7 , XM_010817850.2 , XM_010825144.2 , XM_002697033.5
    Accession Version XM_002697033.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697033.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGACGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCCGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCCGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC CTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697079.1
    CDS146..2524
    Translation

    Target ORF information:

    RefSeq Version XM_002697033.5
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002697033.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGCCA CTCCCTGGAC CCAGATGCAG
    GTGGTCAGAG GCCGAGGCAG CAGAGCCACC GATGGCGCCC TCTACCCGAC GCCCACAGCC
    CCACGCCAGG ATTACGTCTT CTACCTGGAG CCTGAGAAGC TCGAGTCAGG GAAGGGCAAG
    TGTCCCTACG ACCCCAAGCT GGACACAGCC TCAGCCCTCA TCAATGAGGA GCTCTATGCA
    GGCGTGTACA TCGACTTCAT GGGCACGGAC GCAGCCATCT TCCGCACCCT TGGCAAGCAG
    ACGGCCATGC GCACGGATCA GTACAACTCT CGGTGGCTCA ATGACCCTTC TTTCATCCAC
    GCCGAGCTCA TCCCTGACAG TGCCGAGCGC AACGACGACA AGCTCTACTT CTTCTTCCGT
    GAGCGGTCCA TAGAGGCGCC GCAGAGCCCT GCCGTGTACG CCCGCATTGG GCGGATCTGC
    CTGAATGATG ACGGCGGCCA CTGCTGCCTG GTCAACAAGT GGAGCACATT CCTGAAGGCA
    AGGCTGGTCT GCTCTGTGCC AGGCGAGGAT GGCATCGAGA CCCACTTCGA CGAGCTCCAG
    GATGTGTTTG TCCAGCAGAC CCAGGATGTG AGGAACCCAG TCATCTACGC TGTCTTCACC
    TCCTCGGGCT CTGTGTTCCG AGGCTCTGCC GTGTGTGTCT ACTCCATGGC TGACATCCGC
    ATGGTCTTCA ATGGGCCCTT TGCCCACAAG GAGGGCCCCA ACTACCAGTG GATGCCCTTC
    TCAGGAAAGA TGCCCTACCC ACGGCCCGGC ACGTGCCCTG GCGGAACCTT CACGCCGTCC
    ATGAAGTCCA CCAAAGACTA CCCTGATGAA GTCATCAACT TCATGCGCAG CCACCCGCTC
    ATGTACCAGG CCATCTACCC TCTGCAGCGG CGGCCCCTGG TGGTCCGCAC AGGCGCGCCC
    TACCGTCTCA CCACTGTGGC CGTGGACCAG GTGGACGCAG CCGACGGGCG CTATGAGGTG
    CTTTTCCTGG GCACAGACCG CGGGACAGTG CAGAAGGTCA TCGTGCTGCC TAAGGACGAC
    CAGGAGATGG AGGAGCTCAT GCTAGAGGAG GTCGAGGTCT TCAAGGACCC AGCACCTGTT
    AAGACCATGA CCATCTCTTC CAAGAGGCAA CAACTGTACG TGGCCTCAGC CGTGGGCGTC
    ACACAGCTGA GCCTGCACCG CTGTCAGGCA TATGGGGCCG CCTGTGCCGA CTGCTGCCTC
    GCCCGGGACC CCTACTGCGC CTGGGATGGC CAAGCCTGTT CCCGCTACAC AGCGTCCTCT
    AAGAGGCGGA GCCGCCGGCA GGATGTCCGG CACGGGAACC CCATCCGGCA GTGCCGTGGG
    TTCAATTCCA ATGCCAATAA GAACGCCGTG GAGTCTGTGC AGTACGGTGT GGCAGGGAGC
    ACAGCCTTCC TTGAGTGCCA GCCCCGCTCG CCCCAGGCTA CCGTGAAGTG GCTGTTCCAG
    CGAGATCCCA GTGACCGGCG CCGCGAGGTC CACGCGGACG ACCGCTTCCT GCGCACTGAA
    CAAGGCTTGC TGCTCCGCGC CCTGCAGTCT GGTGATAGCG GCCTCTACTC CTGTACAGCC
    ACCGAGAACA ACTTCAAGCA CATCATCACA CGGGTGCAGC TGCATGTCCT GGGCCAGGAA
    GACATCCGTA CGGCCCTCTT CCCACCGCCA GCTGCAAGCA TTCCACCGCC AGCTGCGAGC
    ATTCCACCGC CCCCGGGCAC CGGCCCCCCA AAGCCCCGTT ACCAAGAGCT GGCCCAGCTG
    CTGGCCCAGC CAGAAGTGGG CCTCATCCAC CAGTACTGCC AGGGCTACTG GCGCCACGTG
    CCCCCCAGTC CCAGGGAGGC CCCAGGGGCA CCCAGGCCTC CTAAACCCCA GGACCAGAAA
    AAACCCCGGA ACCGCCGCCA CCACCCACCG GACACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11068
    Clone ID Related Accession (Same CDS sequence) XM_005223007.3 , XM_005196475.3
    Accession Version XM_005223007.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGATT ACGTCTTCTA CCTGGAGCCT
    GAGAAGCTCG AGTCAGGGAA GGGCAAGTGT CCCTACGACC CCAAGCTGGA CACAGCCTCA
    GCCCTCATCA ATGAGGAGCT CTATGCAGGC GTGTACATCG ACTTCATGGG CACGGACGCA
    GCCATCTTCC GCACCCTTGG CAAGCAGACG GCCATGCGCA CGGATCAGTA CAACTCTCGG
    TGGCTCAATG ACCCTTCTTT CATCCACGCC GAGCTCATCC CTGACAGTGC CGAGCGCAAC
    GACGACAAGC TCTACTTCTT CTTCCGTGAG CGGTCCATAG AGGCGCCGCA GAGCCCTGCC
    GTGTACGCCC GCATTGGGCG GATCTGCCTG AATGATGACG GCGGCCACTG CTGCCTGGTC
    AACAAGTGGA GCACATTCCT GAAGGCAAGG CTGGTCTGCT CTGTGCCAGG CGAGGATGGC
    ATCGAGACCC ACTTCGACGA GCTCCAGGAT GTGTTTGTCC AGCAGACCCA GGATGTGAGG
    AACCCAGTCA TCTACGCTGT CTTCACCTCC TCGGGCTCTG TGTTCCGAGG CTCTGCCGTG
    TGTGTCTACT CCATGGCTGA CATCCGCATG GTCTTCAATG GGCCCTTTGC CCACAAGGAG
    GGCCCCAACT ACCAGTGGAT GCCCTTCTCA GGAAAGATGC CCTACCCACG GCCCGGCACG
    TGCCCTGGCG GAACCTTCAC GCCGTCCATG AAGTCCACCA AAGACTACCC TGATGAAGTC
    ATCAACTTCA TGCGCAGCCA CCCGCTCATG TACCAGGCCA TCTACCCTCT GCAGCGGCGG
    CCCCTGGTGG TCCGCACAGG CGCGCCCTAC CGTCTCACCA CTGTGGCCGT GGACCAGGTG
    GACGCAGCCG ACGGGCGCTA TGAGGTGCTT TTCCTGGGCA CAGACCGCGG GACAGTGCAG
    AAGGTCATCG TGCTGCCTAA GGACGACCAG GAGATGGAGG AGCTCATGCT AGAGGAGGTC
    GAGGTCTTCA AGGACCCAGC ACCTGTTAAG ACCATGACCA TCTCTTCCAA GAGGCAACAA
    CTGTACGTGG CCTCAGCCGT GGGCGTCACA CAGCTGAGCC TGCACCGCTG TCAGGCATAT
    GGGGCCGCCT GTGCCGACTG CTGCCTCGCC CGGGACCCCT ACTGCGCCTG GGATGGCCAA
    GCCTGTTCCC GCTACACAGC GTCCTCTAAG AGGCGGAGCC GCCGGCAGGA TGTCCGGCAC
    GGGAACCCCA TCCGGCAGTG CCGTGGGTTC AATTCCAATG CCAATAAGAA CGCCGTGGAG
    TCTGTGCAGT ACGGTGTGGC AGGGAGCACA GCCTTCCTTG AGTGCCAGCC CCGCTCGCCC
    CAGGCTACCG TGAAGTGGCT GTTCCAGCGA GATCCCAGTG ACCGGCGCCG CGAGGTCCAC
    GCGGACGACC GCTTCCTGCG CACTGAACAA GGCTTGCTGC TCCGCGCCCT GCAGTCTGGT
    GATAGCGGCC TCTACTCCTG TACAGCCACC GAGAACAACT TCAAGCACAT CATCACACGG
    GTGCAGCTGC ATGTCCTGGG CCAGGAAGAC ATCCGTACGG CCCTCTTCCC ACCGCCAGCT
    GCAAGCATTC CACCGCCAGC TGCGAGCATT CCACCGCCCC CGGGCACCGG CCCCCCAAAG
    CCCCGTTACC AAGAGCTGGC CCAGCTGCTG GCCCAGCCAG AAGTGGGCCT CATCCACCAG
    TACTGCCAGG GCTACTGGCG CCACGTGCCC CCCAGTCCCA GGGAGGCCCC AGGGGCACCC
    AGGCCTCCTA AACCCCAGGA CCAGAAAAAA CCCCGGAACC GCCGCCACCA CCCACCGGAC
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223064.1
    CDS144..2429
    Translation

    Target ORF information:

    RefSeq Version XM_005223007.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223007.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGATT ACGTCTTCTA CCTGGAGCCT
    GAGAAGCTCG AGTCAGGGAA GGGCAAGTGT CCCTACGACC CCAAGCTGGA CACAGCCTCA
    GCCCTCATCA ATGAGGAGCT CTATGCAGGC GTGTACATCG ACTTCATGGG CACGGACGCA
    GCCATCTTCC GCACCCTTGG CAAGCAGACG GCCATGCGCA CGGATCAGTA CAACTCTCGG
    TGGCTCAATG ACCCTTCTTT CATCCACGCC GAGCTCATCC CTGACAGTGC CGAGCGCAAC
    GACGACAAGC TCTACTTCTT CTTCCGTGAG CGGTCCATAG AGGCGCCGCA GAGCCCTGCC
    GTGTACGCCC GCATTGGGCG GATCTGCCTG AATGATGACG GCGGCCACTG CTGCCTGGTC
    AACAAGTGGA GCACATTCCT GAAGGCAAGG CTGGTCTGCT CTGTGCCAGG CGAGGATGGC
    ATCGAGACCC ACTTCGACGA GCTCCAGGAT GTGTTTGTCC AGCAGACCCA GGATGTGAGG
    AACCCAGTCA TCTACGCTGT CTTCACCTCC TCGGGCTCTG TGTTCCGAGG CTCTGCCGTG
    TGTGTCTACT CCATGGCTGA CATCCGCATG GTCTTCAATG GGCCCTTTGC CCACAAGGAG
    GGCCCCAACT ACCAGTGGAT GCCCTTCTCA GGAAAGATGC CCTACCCACG GCCCGGCACG
    TGCCCTGGCG GAACCTTCAC GCCGTCCATG AAGTCCACCA AAGACTACCC TGATGAAGTC
    ATCAACTTCA TGCGCAGCCA CCCGCTCATG TACCAGGCCA TCTACCCTCT GCAGCGGCGG
    CCCCTGGTGG TCCGCACAGG CGCGCCCTAC CGTCTCACCA CTGTGGCCGT GGACCAGGTG
    GACGCAGCCG ACGGGCGCTA TGAGGTGCTT TTCCTGGGCA CAGACCGCGG GACAGTGCAG
    AAGGTCATCG TGCTGCCTAA GGACGACCAG GAGATGGAGG AGCTCATGCT AGAGGAGGTC
    GAGGTCTTCA AGGACCCAGC ACCTGTTAAG ACCATGACCA TCTCTTCCAA GAGGCAACAA
    CTGTACGTGG CCTCAGCCGT GGGCGTCACA CAGCTGAGCC TGCACCGCTG TCAGGCATAT
    GGGGCCGCCT GTGCCGACTG CTGCCTCGCC CGGGACCCCT ACTGCGCCTG GGATGGCCAA
    GCCTGTTCCC GCTACACAGC GTCCTCTAAG AGGCGGAGCC GCCGGCAGGA TGTCCGGCAC
    GGGAACCCCA TCCGGCAGTG CCGTGGGTTC AATTCCAATG CCAATAAGAA CGCCGTGGAG
    TCTGTGCAGT ACGGTGTGGC AGGGAGCACA GCCTTCCTTG AGTGCCAGCC CCGCTCGCCC
    CAGGCTACCG TGAAGTGGCT GTTCCAGCGA GATCCCAGTG ACCGGCGCCG CGAGGTCCAC
    GCGGACGACC GCTTCCTGCG CACTGAACAA GGCTTGCTGC TCCGCGCCCT GCAGTCTGGT
    GATAGCGGCC TCTACTCCTG TACAGCCACC GAGAACAACT TCAAGCACAT CATCACACGG
    GTGCAGCTGC ATGTCCTGGG CCAGGAAGAC ATCCGTACGG CCCTCTTCCC ACCGCCAGCT
    GCAAGCATTC CACCGCCAGC TGCGAGCATT CCACCGCCCC CGGGCACCGG CCCCCCAAAG
    CCCCGTTACC AAGAGCTGGC CCAGCTGCTG GCCCAGCCAG AAGTGGGCCT CATCCACCAG
    TACTGCCAGG GCTACTGGCG CCACGTGCCC CCCAGTCCCA GGGAGGCCCC AGGGGCACCC
    AGGCCTCCTA AACCCCAGGA CCAGAAAAAA CCCCGGAACC GCCGCCACCA CCCACCGGAC
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11068
    Clone ID Related Accession (Same CDS sequence) XM_005223007.3 , XM_005196475.3
    Accession Version XM_005196475.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196475.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGATT ACGTCTTCTA CCTGGAGCCT
    GAGAAGCTCG AGTCAGGGAA GGGCAAGTGT CCCTACGACC CCAAGCTGGA CACAGCCTCA
    GCCCTCATCA ATGAGGAGCT CTATGCAGGC GTGTACATCG ACTTCATGGG CACGGACGCA
    GCCATCTTCC GCACCCTTGG CAAGCAGACG GCCATGCGCA CGGATCAGTA CAACTCTCGG
    TGGCTCAATG ACCCTTCTTT CATCCACGCC GAGCTCATCC CTGACAGTGC CGAGCGCAAC
    GACGACAAGC TCTACTTCTT CTTCCGTGAG CGGTCCATAG AGGCGCCGCA GAGCCCTGCC
    GTGTACGCCC GCATTGGGCG GATCTGCCTG AATGATGACG GCGGCCACTG CTGCCTGGTC
    AACAAGTGGA GCACATTCCT GAAGGCAAGG CTGGTCTGCT CTGTGCCAGG CGAGGATGGC
    ATCGAGACCC ACTTCGACGA GCTCCAGGAT GTGTTTGTCC AGCAGACCCA GGATGTGAGG
    AACCCAGTCA TCTACGCTGT CTTCACCTCC TCGGGCTCTG TGTTCCGAGG CTCTGCCGTG
    TGTGTCTACT CCATGGCTGA CATCCGCATG GTCTTCAATG GGCCCTTTGC CCACAAGGAG
    GGCCCCAACT ACCAGTGGAT GCCCTTCTCA GGAAAGATGC CCTACCCACG GCCCGGCACG
    TGCCCTGGCG GAACCTTCAC GCCGTCCATG AAGTCCACCA AAGACTACCC TGATGAAGTC
    ATCAACTTCA TGCGCAGCCA CCCGCTCATG TACCAGGCCA TCTACCCTCT GCAGCGGCGG
    CCCCTGGTGG TCCGCACAGG CGCGCCCTAC CGTCTCACCA CTGTGGCCGT GGACCAGGTG
    GACGCAGCCG ACGGGCGCTA TGAGGTGCTT TTCCTGGGCA CAGACCGCGG GACAGTGCAG
    AAGGTCATCG TGCTGCCTAA GGACGACCAG GAGATGGAGG AGCTCATGCT AGAGGAGGTC
    GAGGTCTTCA AGGACCCAGC ACCTGTTAAG ACCATGACCA TCTCTTCCAA GAGGCAACAA
    CTGTACGTGG CCTCAGCCGT GGGCGTCACA CAGCTGAGCC TGCACCGCTG TCAGGCATAT
    GGGGCCGCCT GTGCCGACTG CTGCCTCGCC CGGGACCCCT ACTGCGCCTG GGATGGCCAA
    GCCTGTTCCC GCTACACAGC GTCCTCTAAG AGGCGGAGCC GCCGGCAGGA TGTCCGGCAC
    GGGAACCCCA TCCGGCAGTG CCGTGGGTTC AATTCCAATG CCAATAAGAA CGCCGTGGAG
    TCTGTGCAGT ACGGTGTGGC AGGGAGCACA GCCTTCCTTG AGTGCCAGCC CCGCTCGCCC
    CAGGCTACCG TGAAGTGGCT GTTCCAGCGA GATCCCAGTG ACCGGCGCCG CGAGGTCCAC
    GCGGACGACC GCTTCCTGCG CACTGAACAA GGCTTGCTGC TCCGCGCCCT GCAGTCTGGT
    GATAGCGGCC TCTACTCCTG TACAGCCACC GAGAACAACT TCAAGCACAT CATCACACGG
    GTGCAGCTGC ATGTCCTGGG CCAGGAAGAC ATCCGTACGG CCCTCTTCCC ACCGCCAGCT
    GCAAGCATTC CACCGCCAGC TGCGAGCATT CCACCGCCCC CGGGCACCGG CCCCCCAAAG
    CCCCGTTACC AAGAGCTGGC CCAGCTGCTG GCCCAGCCAG AAGTGGGCCT CATCCACCAG
    TACTGCCAGG GCTACTGGCG CCACGTGCCC CCCAGTCCCA GGGAGGCCCC AGGGGCACCC
    AGGCCTCCTA AACCCCAGGA CCAGAAAAAA CCCCGGAACC GCCGCCACCA CCCACCGGAC
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005196532.1
    CDS144..2429
    Translation

    Target ORF information:

    RefSeq Version XM_005196475.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005196475.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCTGTCG CTGGTCTCCT CCTCTGGGCT TCCCTGCTCA CTGGGGCCTG GCCAGCCGCC 
    CCCAGCCAGG ACTACCTCCC AGCCATGCCC CGGATCCGGC TCTCATATAA AGAGCTTAAG
    GCCACAGGCA CCGCCCACTT CTTCAACTTC CTTCTCAACA CAAGCGACTA CCGAATCCTG
    TTCAAGGACG AGGACCACGA CCGCATGTAT GTGGGCAGCA AGGACTACGT GCTGTCCCTG
    GACCTGCATG ACATCAACCG CGAGCCCCTC ATTATCCACT GGGCAGCCAC CCCACAGCGC
    ATTGAAGAGT GCGTGCTCTC AGGCAAGGAT GGCAACGGTG AGTGCGGGAA CTTCGTCAGG
    CTCATCCAGC CCTGGAACCG AACACACCTG TACGTGTGTG GCACCGGAGC CTACAACCCC
    GTGTGCGCCT ATGTAAACCG TGGCCGCCGC GCGGAGGATT ACGTCTTCTA CCTGGAGCCT
    GAGAAGCTCG AGTCAGGGAA GGGCAAGTGT CCCTACGACC CCAAGCTGGA CACAGCCTCA
    GCCCTCATCA ATGAGGAGCT CTATGCAGGC GTGTACATCG ACTTCATGGG CACGGACGCA
    GCCATCTTCC GCACCCTTGG CAAGCAGACG GCCATGCGCA CGGATCAGTA CAACTCTCGG
    TGGCTCAATG ACCCTTCTTT CATCCACGCC GAGCTCATCC CTGACAGTGC CGAGCGCAAC
    GACGACAAGC TCTACTTCTT CTTCCGTGAG CGGTCCATAG AGGCGCCGCA GAGCCCTGCC
    GTGTACGCCC GCATTGGGCG GATCTGCCTG AATGATGACG GCGGCCACTG CTGCCTGGTC
    AACAAGTGGA GCACATTCCT GAAGGCAAGG CTGGTCTGCT CTGTGCCAGG CGAGGATGGC
    ATCGAGACCC ACTTCGACGA GCTCCAGGAT GTGTTTGTCC AGCAGACCCA GGATGTGAGG
    AACCCAGTCA TCTACGCTGT CTTCACCTCC TCGGGCTCTG TGTTCCGAGG CTCTGCCGTG
    TGTGTCTACT CCATGGCTGA CATCCGCATG GTCTTCAATG GGCCCTTTGC CCACAAGGAG
    GGCCCCAACT ACCAGTGGAT GCCCTTCTCA GGAAAGATGC CCTACCCACG GCCCGGCACG
    TGCCCTGGCG GAACCTTCAC GCCGTCCATG AAGTCCACCA AAGACTACCC TGATGAAGTC
    ATCAACTTCA TGCGCAGCCA CCCGCTCATG TACCAGGCCA TCTACCCTCT GCAGCGGCGG
    CCCCTGGTGG TCCGCACAGG CGCGCCCTAC CGTCTCACCA CTGTGGCCGT GGACCAGGTG
    GACGCAGCCG ACGGGCGCTA TGAGGTGCTT TTCCTGGGCA CAGACCGCGG GACAGTGCAG
    AAGGTCATCG TGCTGCCTAA GGACGACCAG GAGATGGAGG AGCTCATGCT AGAGGAGGTC
    GAGGTCTTCA AGGACCCAGC ACCTGTTAAG ACCATGACCA TCTCTTCCAA GAGGCAACAA
    CTGTACGTGG CCTCAGCCGT GGGCGTCACA CAGCTGAGCC TGCACCGCTG TCAGGCATAT
    GGGGCCGCCT GTGCCGACTG CTGCCTCGCC CGGGACCCCT ACTGCGCCTG GGATGGCCAA
    GCCTGTTCCC GCTACACAGC GTCCTCTAAG AGGCGGAGCC GCCGGCAGGA TGTCCGGCAC
    GGGAACCCCA TCCGGCAGTG CCGTGGGTTC AATTCCAATG CCAATAAGAA CGCCGTGGAG
    TCTGTGCAGT ACGGTGTGGC AGGGAGCACA GCCTTCCTTG AGTGCCAGCC CCGCTCGCCC
    CAGGCTACCG TGAAGTGGCT GTTCCAGCGA GATCCCAGTG ACCGGCGCCG CGAGGTCCAC
    GCGGACGACC GCTTCCTGCG CACTGAACAA GGCTTGCTGC TCCGCGCCCT GCAGTCTGGT
    GATAGCGGCC TCTACTCCTG TACAGCCACC GAGAACAACT TCAAGCACAT CATCACACGG
    GTGCAGCTGC ATGTCCTGGG CCAGGAAGAC ATCCGTACGG CCCTCTTCCC ACCGCCAGCT
    GCAAGCATTC CACCGCCAGC TGCGAGCATT CCACCGCCCC CGGGCACCGG CCCCCCAAAG
    CCCCGTTACC AAGAGCTGGC CCAGCTGCTG GCCCAGCCAG AAGTGGGCCT CATCCACCAG
    TACTGCCAGG GCTACTGGCG CCACGTGCCC CCCAGTCCCA GGGAGGCCCC AGGGGCACCC
    AGGCCTCCTA AACCCCAGGA CCAGAAAAAA CCCCGGAACC GCCGCCACCA CCCACCGGAC
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51943
    Clone ID Related Accession (Same CDS sequence) XM_010817851.2 , XM_010825145.2
    Accession Version XM_010817851.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCAGGTGG TCAGAGGCCG AGGCAGCAGA GCCACCGATG GCGCCCTCTA CCCGACGCCC 
    ACAGCCCCAC GCCAGGATTA CGTCTTCTAC CTGGAGCCTG AGAAGCTCGA GTCAGGGAAG
    GGCAAGTGTC CCTACGACCC CAAGCTGGAC ACAGCCTCAG CCCTCATCAA TGAGGAGCTC
    TATGCAGGCG TGTACATCGA CTTCATGGGC ACGGACGCAG CCATCTTCCG CACCCTTGGC
    AAGCAGACGG CCATGCGCAC GGATCAGTAC AACTCTCGGT GGCTCAATGA CCCTTCTTTC
    ATCCACGCCG AGCTCATCCC TGACAGTGCC GAGCGCAACG ACGACAAGCT CTACTTCTTC
    TTCCGTGAGC GGTCCATAGA GGCGCCGCAG AGCCCTGCCG TGTACGCCCG CATTGGGCGG
    ATCTGCCTGA ATGATGACGG CGGCCACTGC TGCCTGGTCA ACAAGTGGAG CACATTCCTG
    AAGGCAAGGC TGGTCTGCTC TGTGCCAGGC GAGGATGGCA TCGAGACCCA CTTCGACGAG
    CTCCAGGATG TGTTTGTCCA GCAGACCCAG GATGTGAGGA ACCCAGTCAT CTACGCTGTC
    TTCACCTCCT CGGGCTCTGT GTTCCGAGGC TCTGCCGTGT GTGTCTACTC CATGGCTGAC
    ATCCGCATGG TCTTCAATGG GCCCTTTGCC CACAAGGAGG GCCCCAACTA CCAGTGGATG
    CCCTTCTCAG GAAAGATGCC CTACCCACGG CCCGGCACGT GCCCTGGCGG AACCTTCACG
    CCGTCCATGA AGTCCACCAA AGACTACCCT GATGAAGTCA TCAACTTCAT GCGCAGCCAC
    CCGCTCATGT ACCAGGCCAT CTACCCTCTG CAGCGGCGGC CCCTGGTGGT CCGCACAGGC
    GCGCCCTACC GTCTCACCAC TGTGGCCGTG GACCAGGTGG ACGCAGCCGA CGGGCGCTAT
    GAGGTGCTTT TCCTGGGCAC AGACCGCGGG ACAGTGCAGA AGGTCATCGT GCTGCCTAAG
    GACGACCAGG AGATGGAGGA GCTCATGCTA GAGGAGGTCG AGGTCTTCAA GGACCCAGCA
    CCTGTTAAGA CCATGACCAT CTCTTCCAAG AGGCAACAAC TGTACGTGGC CTCAGCCGTG
    GGCGTCACAC AGCTGAGCCT GCACCGCTGT CAGGCATATG GGGCCGCCTG TGCCGACTGC
    TGCCTCGCCC GGGACCCCTA CTGCGCCTGG GATGGCCAAG CCTGTTCCCG CTACACAGCG
    TCCTCTAAGA GGCGGAGCCG CCGGCAGGAT GTCCGGCACG GGAACCCCAT CCGGCAGTGC
    CGTGGGTTCA ATTCCAATGC CAATAAGAAC GCCGTGGAGT CTGTGCAGTA CGGTGTGGCA
    GGGAGCACAG CCTTCCTTGA GTGCCAGCCC CGCTCGCCCC AGGCTACCGT GAAGTGGCTG
    TTCCAGCGAG ATCCCAGTGA CCGGCGCCGC GAGGTCCACG CGGACGACCG CTTCCTGCGC
    ACTGAACAAG GCTTGCTGCT CCGCGCCCTG CAGTCTGGTG ATAGCGGCCT CTACTCCTGT
    ACAGCCACCG AGAACAACTT CAAGCACATC ATCACACGGG TGCAGCTGCA TGTCCTGGGC
    CAGGAAGACA TCCGTACGGC CCTCTTCCCA CCGCCAGCTG CAAGCATTCC ACCGCCAGCT
    GCGAGCATTC CACCGCCCCC GGGCACCGGC CCCCCAAAGC CCCGTTACCA AGAGCTGGCC
    CAGCTGCTGG CCCAGCCAGA AGTGGGCCTC ATCCACCAGT ACTGCCAGGG CTACTGGCGC
    CACGTGCCCC CCAGTCCCAG GGAGGCCCCA GGGGCACCCA GGCCTCCTAA ACCCCAGGAC
    CAGAAAAAAC CCCGGAACCG CCGCCACCAC CCACCGGACA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816153.1
    CDS131..2035
    Translation

    Target ORF information:

    RefSeq Version XM_010817851.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817851.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCAGGTGG TCAGAGGCCG AGGCAGCAGA GCCACCGATG GCGCCCTCTA CCCGACGCCC 
    ACAGCCCCAC GCCAGGATTA CGTCTTCTAC CTGGAGCCTG AGAAGCTCGA GTCAGGGAAG
    GGCAAGTGTC CCTACGACCC CAAGCTGGAC ACAGCCTCAG CCCTCATCAA TGAGGAGCTC
    TATGCAGGCG TGTACATCGA CTTCATGGGC ACGGACGCAG CCATCTTCCG CACCCTTGGC
    AAGCAGACGG CCATGCGCAC GGATCAGTAC AACTCTCGGT GGCTCAATGA CCCTTCTTTC
    ATCCACGCCG AGCTCATCCC TGACAGTGCC GAGCGCAACG ACGACAAGCT CTACTTCTTC
    TTCCGTGAGC GGTCCATAGA GGCGCCGCAG AGCCCTGCCG TGTACGCCCG CATTGGGCGG
    ATCTGCCTGA ATGATGACGG CGGCCACTGC TGCCTGGTCA ACAAGTGGAG CACATTCCTG
    AAGGCAAGGC TGGTCTGCTC TGTGCCAGGC GAGGATGGCA TCGAGACCCA CTTCGACGAG
    CTCCAGGATG TGTTTGTCCA GCAGACCCAG GATGTGAGGA ACCCAGTCAT CTACGCTGTC
    TTCACCTCCT CGGGCTCTGT GTTCCGAGGC TCTGCCGTGT GTGTCTACTC CATGGCTGAC
    ATCCGCATGG TCTTCAATGG GCCCTTTGCC CACAAGGAGG GCCCCAACTA CCAGTGGATG
    CCCTTCTCAG GAAAGATGCC CTACCCACGG CCCGGCACGT GCCCTGGCGG AACCTTCACG
    CCGTCCATGA AGTCCACCAA AGACTACCCT GATGAAGTCA TCAACTTCAT GCGCAGCCAC
    CCGCTCATGT ACCAGGCCAT CTACCCTCTG CAGCGGCGGC CCCTGGTGGT CCGCACAGGC
    GCGCCCTACC GTCTCACCAC TGTGGCCGTG GACCAGGTGG ACGCAGCCGA CGGGCGCTAT
    GAGGTGCTTT TCCTGGGCAC AGACCGCGGG ACAGTGCAGA AGGTCATCGT GCTGCCTAAG
    GACGACCAGG AGATGGAGGA GCTCATGCTA GAGGAGGTCG AGGTCTTCAA GGACCCAGCA
    CCTGTTAAGA CCATGACCAT CTCTTCCAAG AGGCAACAAC TGTACGTGGC CTCAGCCGTG
    GGCGTCACAC AGCTGAGCCT GCACCGCTGT CAGGCATATG GGGCCGCCTG TGCCGACTGC
    TGCCTCGCCC GGGACCCCTA CTGCGCCTGG GATGGCCAAG CCTGTTCCCG CTACACAGCG
    TCCTCTAAGA GGCGGAGCCG CCGGCAGGAT GTCCGGCACG GGAACCCCAT CCGGCAGTGC
    CGTGGGTTCA ATTCCAATGC CAATAAGAAC GCCGTGGAGT CTGTGCAGTA CGGTGTGGCA
    GGGAGCACAG CCTTCCTTGA GTGCCAGCCC CGCTCGCCCC AGGCTACCGT GAAGTGGCTG
    TTCCAGCGAG ATCCCAGTGA CCGGCGCCGC GAGGTCCACG CGGACGACCG CTTCCTGCGC
    ACTGAACAAG GCTTGCTGCT CCGCGCCCTG CAGTCTGGTG ATAGCGGCCT CTACTCCTGT
    ACAGCCACCG AGAACAACTT CAAGCACATC ATCACACGGG TGCAGCTGCA TGTCCTGGGC
    CAGGAAGACA TCCGTACGGC CCTCTTCCCA CCGCCAGCTG CAAGCATTCC ACCGCCAGCT
    GCGAGCATTC CACCGCCCCC GGGCACCGGC CCCCCAAAGC CCCGTTACCA AGAGCTGGCC
    CAGCTGCTGG CCCAGCCAGA AGTGGGCCTC ATCCACCAGT ACTGCCAGGG CTACTGGCGC
    CACGTGCCCC CCAGTCCCAG GGAGGCCCCA GGGGCACCCA GGCCTCCTAA ACCCCAGGAC
    CAGAAAAAAC CCCGGAACCG CCGCCACCAC CCACCGGACA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51943
    Clone ID Related Accession (Same CDS sequence) XM_010817851.2 , XM_010825145.2
    Accession Version XM_010825145.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-3F isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCAGGTGG TCAGAGGCCG AGGCAGCAGA GCCACCGATG GCGCCCTCTA CCCGACGCCC 
    ACAGCCCCAC GCCAGGATTA CGTCTTCTAC CTGGAGCCTG AGAAGCTCGA GTCAGGGAAG
    GGCAAGTGTC CCTACGACCC CAAGCTGGAC ACAGCCTCAG CCCTCATCAA TGAGGAGCTC
    TATGCAGGCG TGTACATCGA CTTCATGGGC ACGGACGCAG CCATCTTCCG CACCCTTGGC
    AAGCAGACGG CCATGCGCAC GGATCAGTAC AACTCTCGGT GGCTCAATGA CCCTTCTTTC
    ATCCACGCCG AGCTCATCCC TGACAGTGCC GAGCGCAACG ACGACAAGCT CTACTTCTTC
    TTCCGTGAGC GGTCCATAGA GGCGCCGCAG AGCCCTGCCG TGTACGCCCG CATTGGGCGG
    ATCTGCCTGA ATGATGACGG CGGCCACTGC TGCCTGGTCA ACAAGTGGAG CACATTCCTG
    AAGGCAAGGC TGGTCTGCTC TGTGCCAGGC GAGGATGGCA TCGAGACCCA CTTCGACGAG
    CTCCAGGATG TGTTTGTCCA GCAGACCCAG GATGTGAGGA ACCCAGTCAT CTACGCTGTC
    TTCACCTCCT CGGGCTCTGT GTTCCGAGGC TCTGCCGTGT GTGTCTACTC CATGGCTGAC
    ATCCGCATGG TCTTCAATGG GCCCTTTGCC CACAAGGAGG GCCCCAACTA CCAGTGGATG
    CCCTTCTCAG GAAAGATGCC CTACCCACGG CCCGGCACGT GCCCTGGCGG AACCTTCACG
    CCGTCCATGA AGTCCACCAA AGACTACCCT GATGAAGTCA TCAACTTCAT GCGCAGCCAC
    CCGCTCATGT ACCAGGCCAT CTACCCTCTG CAGCGGCGGC CCCTGGTGGT CCGCACAGGC
    GCGCCCTACC GTCTCACCAC TGTGGCCGTG GACCAGGTGG ACGCAGCCGA CGGGCGCTAT
    GAGGTGCTTT TCCTGGGCAC AGACCGCGGG ACAGTGCAGA AGGTCATCGT GCTGCCTAAG
    GACGACCAGG AGATGGAGGA GCTCATGCTA GAGGAGGTCG AGGTCTTCAA GGACCCAGCA
    CCTGTTAAGA CCATGACCAT CTCTTCCAAG AGGCAACAAC TGTACGTGGC CTCAGCCGTG
    GGCGTCACAC AGCTGAGCCT GCACCGCTGT CAGGCATATG GGGCCGCCTG TGCCGACTGC
    TGCCTCGCCC GGGACCCCTA CTGCGCCTGG GATGGCCAAG CCTGTTCCCG CTACACAGCG
    TCCTCTAAGA GGCGGAGCCG CCGGCAGGAT GTCCGGCACG GGAACCCCAT CCGGCAGTGC
    CGTGGGTTCA ATTCCAATGC CAATAAGAAC GCCGTGGAGT CTGTGCAGTA CGGTGTGGCA
    GGGAGCACAG CCTTCCTTGA GTGCCAGCCC CGCTCGCCCC AGGCTACCGT GAAGTGGCTG
    TTCCAGCGAG ATCCCAGTGA CCGGCGCCGC GAGGTCCACG CGGACGACCG CTTCCTGCGC
    ACTGAACAAG GCTTGCTGCT CCGCGCCCTG CAGTCTGGTG ATAGCGGCCT CTACTCCTGT
    ACAGCCACCG AGAACAACTT CAAGCACATC ATCACACGGG TGCAGCTGCA TGTCCTGGGC
    CAGGAAGACA TCCGTACGGC CCTCTTCCCA CCGCCAGCTG CAAGCATTCC ACCGCCAGCT
    GCGAGCATTC CACCGCCCCC GGGCACCGGC CCCCCAAAGC CCCGTTACCA AGAGCTGGCC
    CAGCTGCTGG CCCAGCCAGA AGTGGGCCTC ATCCACCAGT ACTGCCAGGG CTACTGGCGC
    CACGTGCCCC CCAGTCCCAG GGAGGCCCCA GGGGCACCCA GGCCTCCTAA ACCCCAGGAC
    CAGAAAAAAC CCCGGAACCG CCGCCACCAC CCACCGGACA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010823447.1
    CDS131..2035
    Translation

    Target ORF information:

    RefSeq Version XM_010825145.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 3F (SEMA3F), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010825145.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCAGGTGG TCAGAGGCCG AGGCAGCAGA GCCACCGATG GCGCCCTCTA CCCGACGCCC 
    ACAGCCCCAC GCCAGGATTA CGTCTTCTAC CTGGAGCCTG AGAAGCTCGA GTCAGGGAAG
    GGCAAGTGTC CCTACGACCC CAAGCTGGAC ACAGCCTCAG CCCTCATCAA TGAGGAGCTC
    TATGCAGGCG TGTACATCGA CTTCATGGGC ACGGACGCAG CCATCTTCCG CACCCTTGGC
    AAGCAGACGG CCATGCGCAC GGATCAGTAC AACTCTCGGT GGCTCAATGA CCCTTCTTTC
    ATCCACGCCG AGCTCATCCC TGACAGTGCC GAGCGCAACG ACGACAAGCT CTACTTCTTC
    TTCCGTGAGC GGTCCATAGA GGCGCCGCAG AGCCCTGCCG TGTACGCCCG CATTGGGCGG
    ATCTGCCTGA ATGATGACGG CGGCCACTGC TGCCTGGTCA ACAAGTGGAG CACATTCCTG
    AAGGCAAGGC TGGTCTGCTC TGTGCCAGGC GAGGATGGCA TCGAGACCCA CTTCGACGAG
    CTCCAGGATG TGTTTGTCCA GCAGACCCAG GATGTGAGGA ACCCAGTCAT CTACGCTGTC
    TTCACCTCCT CGGGCTCTGT GTTCCGAGGC TCTGCCGTGT GTGTCTACTC CATGGCTGAC
    ATCCGCATGG TCTTCAATGG GCCCTTTGCC CACAAGGAGG GCCCCAACTA CCAGTGGATG
    CCCTTCTCAG GAAAGATGCC CTACCCACGG CCCGGCACGT GCCCTGGCGG AACCTTCACG
    CCGTCCATGA AGTCCACCAA AGACTACCCT GATGAAGTCA TCAACTTCAT GCGCAGCCAC
    CCGCTCATGT ACCAGGCCAT CTACCCTCTG CAGCGGCGGC CCCTGGTGGT CCGCACAGGC
    GCGCCCTACC GTCTCACCAC TGTGGCCGTG GACCAGGTGG ACGCAGCCGA CGGGCGCTAT
    GAGGTGCTTT TCCTGGGCAC AGACCGCGGG ACAGTGCAGA AGGTCATCGT GCTGCCTAAG
    GACGACCAGG AGATGGAGGA GCTCATGCTA GAGGAGGTCG AGGTCTTCAA GGACCCAGCA
    CCTGTTAAGA CCATGACCAT CTCTTCCAAG AGGCAACAAC TGTACGTGGC CTCAGCCGTG
    GGCGTCACAC AGCTGAGCCT GCACCGCTGT CAGGCATATG GGGCCGCCTG TGCCGACTGC
    TGCCTCGCCC GGGACCCCTA CTGCGCCTGG GATGGCCAAG CCTGTTCCCG CTACACAGCG
    TCCTCTAAGA GGCGGAGCCG CCGGCAGGAT GTCCGGCACG GGAACCCCAT CCGGCAGTGC
    CGTGGGTTCA ATTCCAATGC CAATAAGAAC GCCGTGGAGT CTGTGCAGTA CGGTGTGGCA
    GGGAGCACAG CCTTCCTTGA GTGCCAGCCC CGCTCGCCCC AGGCTACCGT GAAGTGGCTG
    TTCCAGCGAG ATCCCAGTGA CCGGCGCCGC GAGGTCCACG CGGACGACCG CTTCCTGCGC
    ACTGAACAAG GCTTGCTGCT CCGCGCCCTG CAGTCTGGTG ATAGCGGCCT CTACTCCTGT
    ACAGCCACCG AGAACAACTT CAAGCACATC ATCACACGGG TGCAGCTGCA TGTCCTGGGC
    CAGGAAGACA TCCGTACGGC CCTCTTCCCA CCGCCAGCTG CAAGCATTCC ACCGCCAGCT
    GCGAGCATTC CACCGCCCCC GGGCACCGGC CCCCCAAAGC CCCGTTACCA AGAGCTGGCC
    CAGCTGCTGG CCCAGCCAGA AGTGGGCCTC ATCCACCAGT ACTGCCAGGG CTACTGGCGC
    CACGTGCCCC CCAGTCCCAG GGAGGCCCCA GGGGCACCCA GGCCTCCTAA ACCCCAGGAC
    CAGAAAAAAC CCCGGAACCG CCGCCACCAC CCACCGGACA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.