sema3fb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema3fb
Entrez Gene ID	799945
Full Name	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb
Synonyms	sb:cb931
General protein information	Preferred Names sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb Names sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb semaphorin-3F sema3f2 semaphorin 3fb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306194.2	XP_009304469.1	semaphorin-3F isoform X1
XM_009306194.3	XP_009304469.2	semaphorin-3F isoform X1
XM_021472495.1	XP_021328170.1	semaphorin-3F isoform X3
XM_021479878.1	XP_021335553.1	semaphorin-3F isoform X2
XM_021472496.1	XP_021328171.1	semaphorin-3F isoform X4
XM_005172909.3	XP_005172966.1	semaphorin-3F isoform X2
XM_005172909.4	XP_005172966.2	semaphorin-3F isoform X2
XM_021479877.1	XP_021335552.1	semaphorin-3F isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SEMA3F	XP_003309850.2
	SEMA3F	XP_001103281.1
Rattus norvegicus (Norway rat)	Sema3f	NP_001101655.1
Danio rerio (zebrafish)	sema3fb	XP_001921106.2
Canis lupus familiaris (dog)	SEMA3F	XP_541879.1
Mus musculus (house mouse)	Sema3f	NP_035479.2
Xenopus tropicalis (tropical clawed frog)	sema3f	NP_001011157.1
Homo sapiens (human)	SEMA3F	NP_004177.3
Bos taurus (cattle)	SEMA3F	XP_002697079.1
Danio rerio (zebrafish)	sema3fa	NP_001014822.1
Gallus gallus (chicken)	SEMA3F	NP_989589.1

Gene Ontology

The following sema3fb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema3fb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17258	XM_005172909.3 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17258	XM_021479878.1 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17259	XM_009306194.2 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17259	XM_021479877.1 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa145325	XM_009306194.3 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa145326	XM_005172909.4 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa145327	XM_021472495.1 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa145328	XM_021472496.1 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema3fb ( XM_021479878.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335553.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17258
Clone ID Related Accession (Same CDS sequence)	XM_021479878.1 , XM_005172909.3
Accession Version	XM_021479878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-3F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG 
AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC 
AATTCCAACA TTAATAAGAA CACACTGGAG ACAGTGCAGT ATGGGGTGGA GGGCAGCAGC 
ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA 
GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA 
GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA 
GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC 
GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC 
TGGACCCCCA GTGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG 
GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC 
ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT 
CACGGGGACG AGGAGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335553.1
CDS	396..2759
Translation	MLLDSLWPVQVLLVLSVLTLVLGNEPQNAPRVFLSFKDLRSTGTAHYFSFLLNTSDYRILRMDEDHDRMYVGSKDYILSLDLHDINREPLIIHWPVSPQRKTECVLSGKDTNGECGNFIRLIEPWNRTHLYICGTGAYNPVCTYVNRGRKPMTAMHLQMPQTRGRASRAAEPETVFDETGLQEYVFHLEPGKEDSGKGKCPYDPKLNSVSALINGELYAGVYIDFMGTDSAIFRTLGKNAVMRTDQYNSRWLNDPSFVHVHLIPDSGEKNDDKLYFFFREKSSEMGQSPKSQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGADGIETHFDELRDVYIQPTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRNHPTMYNSVYPVHKRPLVVRTNVDYEFTTITVDQVTAADGNYEVLFLGTDRGTVQKVIVLPSDDLQTEELVLEEVEVFKVPTSITTMKISSKRQQLYVASAVGVTHMALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRRSRRQDVKYGNPIRQCRGYNSNINKNTLETVQYGVEGSSTFLECQARSPHAAIKWHLHRDNSDRRREVRSDGRVLKTDQGLLLRSLQSSDSGIYICTATEKNFKHTLVKLQLLVLTNQAVNNILVDTGRPVVSPLQSSAWTPSAGQYKDLLTILSQPEMGLINQYCQDYWQYGDPLTGVIKAKDLKELKEQKKPRNRRHHGDEEET

Target ORF information:

RefSeq Version	XM_021479878.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479878.1

ORF Insert Sequence:

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG 
AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC 
AATTCCAACA TTAATAAGAA CACACTGGAG ACAGTGCAGT ATGGGGTGGA GGGCAGCAGC 
ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA 
GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA 
GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA 
GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC 
GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC 
TGGACCCCCA GTGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG 
GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC 
ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT 
CACGGGGACG AGGAGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fb ( XM_005172909.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172966.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17258
Clone ID Related Accession (Same CDS sequence)	XM_021479878.1 , XM_005172909.3
Accession Version	XM_005172909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-3F isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG 
AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC 
AATTCCAACA TTAATAAGAA CACACTGGAG ACAGTGCAGT ATGGGGTGGA GGGCAGCAGC 
ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA 
GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA 
GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA 
GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC 
GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC 
TGGACCCCCA GTGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG 
GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC 
ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT 
CACGGGGACG AGGAGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172966.1
CDS	375..2738
Translation	MLLDSLWPVQVLLVLSVLTLVLGNEPQNAPRVFLSFKDLRSTGTAHYFSFLLNTSDYRILRMDEDHDRMYVGSKDYILSLDLHDINREPLIIHWPVSPQRKTECVLSGKDTNGECGNFIRLIEPWNRTHLYICGTGAYNPVCTYVNRGRKPMTAMHLQMPQTRGRASRAAEPETVFDETGLQEYVFHLEPGKEDSGKGKCPYDPKLNSVSALINGELYAGVYIDFMGTDSAIFRTLGKNAVMRTDQYNSRWLNDPSFVHVHLIPDSGEKNDDKLYFFFREKSSEMGQSPKSQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGADGIETHFDELRDVYIQPTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRNHPTMYNSVYPVHKRPLVVRTNVDYEFTTITVDQVTAADGNYEVLFLGTDRGTVQKVIVLPSDDLQTEELVLEEVEVFKVPTSITTMKISSKRQQLYVASAVGVTHMALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRRSRRQDVKYGNPIRQCRGYNSNINKNTLETVQYGVEGSSTFLECQARSPHAAIKWHLHRDNSDRRREVRSDGRVLKTDQGLLLRSLQSSDSGIYICTATEKNFKHTLVKLQLLVLTNQAVNNILVDTGRPVVSPLQSSAWTPSAGQYKDLLTILSQPEMGLINQYCQDYWQYGDPLTGVIKAKDLKELKEQKKPRNRRHHGDEEET

Target ORF information:

RefSeq Version	XM_005172909.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172909.3

ORF Insert Sequence:

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG 
AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC 
AATTCCAACA TTAATAAGAA CACACTGGAG ACAGTGCAGT ATGGGGTGGA GGGCAGCAGC 
ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA 
GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA 
GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA 
GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC 
GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC 
TGGACCCCCA GTGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG 
GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC 
ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT 
CACGGGGACG AGGAGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fb ( XM_009306194.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304469.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17259
Clone ID Related Accession (Same CDS sequence)	XM_009306194.2 , XM_021479877.1
Accession Version	XM_009306194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-3F isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG 
AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG 
TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACAGTGCA GTATGGGGTG 
GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG 
CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG 
AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC 
TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG 
ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC 
CAGTCCAGCG CCTGGACCCC CAGTGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC 
CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT 
CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA 
AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304469.1
CDS	375..2750
Translation	MLLDSLWPVQVLLVLSVLTLVLGNEPQNAPRVFLSFKDLRSTGTAHYFSFLLNTSDYRILRMDEDHDRMYVGSKDYILSLDLHDINREPLIIHWPVSPQRKTECVLSGKDTNGECGNFIRLIEPWNRTHLYICGTGAYNPVCTYVNRGRKPMTAMHLQMPQTRGRASRAAEPETVFDETGLQEYVFHLEPGKEDSGKGKCPYDPKLNSVSALINGELYAGVYIDFMGTDSAIFRTLGKNAVMRTDQYNSRWLNDPSFVHVHLIPDSGEKNDDKLYFFFREKSSEMGQSPKSQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGADGIETHFDELRDVYIQPTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRNHPTMYNSVYPVHKRPLVVRTNVDYEFTTITVDQVTAADGNYEVLFLGTDRGTVQKVIVLPSDDLQTEELVLEEVEVFKVPTSITTMKISSKRQQLYVASAVGVTHMALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRLPFRRSRRQDVKYGNPIRQCRGYNSNINKNTLETVQYGVEGSSTFLECQARSPHAAIKWHLHRDNSDRRREVRSDGRVLKTDQGLLLRSLQSSDSGIYICTATEKNFKHTLVKLQLLVLTNQAVNNILVDTGRPVVSPLQSSAWTPSAGQYKDLLTILSQPEMGLINQYCQDYWQYGDPLTGVIKAKDLKELKEQKKPRNRRHHGDEEET

Target ORF information:

RefSeq Version	XM_009306194.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306194.2

ORF Insert Sequence:

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG 
AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG 
TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACAGTGCA GTATGGGGTG 
GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG 
CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG 
AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC 
TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG 
ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC 
CAGTCCAGCG CCTGGACCCC CAGTGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC 
CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT 
CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA 
AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fb ( XM_021479877.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335552.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17259
Clone ID Related Accession (Same CDS sequence)	XM_009306194.2 , XM_021479877.1
Accession Version	XM_021479877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-3F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG 
AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG 
TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACAGTGCA GTATGGGGTG 
GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG 
CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG 
AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC 
TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG 
ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC 
CAGTCCAGCG CCTGGACCCC CAGTGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC 
CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT 
CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA 
AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335552.1
CDS	397..2772
Translation	MLLDSLWPVQVLLVLSVLTLVLGNEPQNAPRVFLSFKDLRSTGTAHYFSFLLNTSDYRILRMDEDHDRMYVGSKDYILSLDLHDINREPLIIHWPVSPQRKTECVLSGKDTNGECGNFIRLIEPWNRTHLYICGTGAYNPVCTYVNRGRKPMTAMHLQMPQTRGRASRAAEPETVFDETGLQEYVFHLEPGKEDSGKGKCPYDPKLNSVSALINGELYAGVYIDFMGTDSAIFRTLGKNAVMRTDQYNSRWLNDPSFVHVHLIPDSGEKNDDKLYFFFREKSSEMGQSPKSQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGADGIETHFDELRDVYIQPTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRNHPTMYNSVYPVHKRPLVVRTNVDYEFTTITVDQVTAADGNYEVLFLGTDRGTVQKVIVLPSDDLQTEELVLEEVEVFKVPTSITTMKISSKRQQLYVASAVGVTHMALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRLPFRRSRRQDVKYGNPIRQCRGYNSNINKNTLETVQYGVEGSSTFLECQARSPHAAIKWHLHRDNSDRRREVRSDGRVLKTDQGLLLRSLQSSDSGIYICTATEKNFKHTLVKLQLLVLTNQAVNNILVDTGRPVVSPLQSSAWTPSAGQYKDLLTILSQPEMGLINQYCQDYWQYGDPLTGVIKAKDLKELKEQKKPRNRRHHGDEEET

Target ORF information:

RefSeq Version	XM_021479877.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479877.1

ORF Insert Sequence:

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG 
AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG 
TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACAGTGCA GTATGGGGTG 
GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG 
CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG 
AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC 
TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG 
ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC 
CAGTCCAGCG CCTGGACCCC CAGTGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC 
CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT 
CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA 
AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fb ( XM_009306194.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304469.2 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa145325
Clone ID Related Accession (Same CDS sequence)	XM_009306194.3
Accession Version	XM_009306194.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-3F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394824.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306194.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAAACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GCGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CGGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCTCTGGTGG TCAGGACCAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAAAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCCTG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAA 
AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG 
TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACGGTGCA GTATGGGGTG 
GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG 
CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG 
AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC 
TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG 
ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC 
CAGTCCAGCG CCTGGACCCC CAGCGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC 
CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT 
CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA 
AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304469.2
CDS	397..2772
Translation	MLLDSLWPVQVLLVLSVLTLVLGNEPQNAPRVFLSFKDLRSTGTAHYFSFLLNTSDYRILRMDEDHDRMYVGSKDYILSLDLHDINREPLIIHWPVSPQRKTECVLSGKDTNGECGNFIRLIEPWNRTHLYICGTGAYNPVCTYVNRGRKPMTAMHLQMPQTRGRASRAAEPETVFDETGLQEYVFHLEPGKEDSGKGKCPYDPKLNSVSALINGELYAGVYIDFMGTDSAIFRTLGKNAVMRTDQYNSRWLNDPSFVHVHLIPDSGEKNDDKLYFFFREKSSEMGQSPKSQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGADGIETHFDELRDVYIQPTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRNHPTMYNSVYPVHKRPLVVRTNVDYEFTTITVDQVTAADGNYEVLFLGTDKGTVQKVIVLPSDDLQTEELVLEEVEVFKVPTSITTMKISSKRQQLYVASAVGVTHMALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRLPFRRSRRQDVKYGNPIRQCRGYNSNINKNTLETVQYGVEGSSTFLECQARSPHAAIKWHLHRDNSDRRREVRSDGRVLKTDQGLLLRSLQSSDSGIYICTATEKNFKHTLVKLQLLVLTNQAVNNILVDTGRPVVSPLQSSAWTPSAGQYKDLLTILSQPEMGLINQYCQDYWQYGDPLTGVIKAKDLKELKEQKKPRNRRHHGDEEET

Target ORF information:

RefSeq Version	XM_009306194.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306194.3

ORF Insert Sequence:

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAAACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GCGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CGGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCTCTGGTGG TCAGGACCAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAAAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCCTG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAA 
AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG 
TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACGGTGCA GTATGGGGTG 
GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG 
CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG 
AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC 
TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG 
ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC 
CAGTCCAGCG CCTGGACCCC CAGCGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC 
CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT 
CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA 
AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fb ( XM_005172909.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172966.2 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa145326
Clone ID Related Accession (Same CDS sequence)	XM_005172909.4
Accession Version	XM_005172909.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-3F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172909.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAAACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GCGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CGGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCTCTGGTGG TCAGGACCAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAAAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCCTG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAA 
AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC 
AATTCCAACA TTAATAAGAA CACACTGGAG ACGGTGCAGT ATGGGGTGGA GGGCAGCAGC 
ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA 
GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA 
GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA 
GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC 
GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC 
TGGACCCCCA GCGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG 
GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC 
ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT 
CACGGGGACG AGGAGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172966.2
CDS	396..2759
Translation	MLLDSLWPVQVLLVLSVLTLVLGNEPQNAPRVFLSFKDLRSTGTAHYFSFLLNTSDYRILRMDEDHDRMYVGSKDYILSLDLHDINREPLIIHWPVSPQRKTECVLSGKDTNGECGNFIRLIEPWNRTHLYICGTGAYNPVCTYVNRGRKPMTAMHLQMPQTRGRASRAAEPETVFDETGLQEYVFHLEPGKEDSGKGKCPYDPKLNSVSALINGELYAGVYIDFMGTDSAIFRTLGKNAVMRTDQYNSRWLNDPSFVHVHLIPDSGEKNDDKLYFFFREKSSEMGQSPKSQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGADGIETHFDELRDVYIQPTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRNHPTMYNSVYPVHKRPLVVRTNVDYEFTTITVDQVTAADGNYEVLFLGTDKGTVQKVIVLPSDDLQTEELVLEEVEVFKVPTSITTMKISSKRQQLYVASAVGVTHMALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRRSRRQDVKYGNPIRQCRGYNSNINKNTLETVQYGVEGSSTFLECQARSPHAAIKWHLHRDNSDRRREVRSDGRVLKTDQGLLLRSLQSSDSGIYICTATEKNFKHTLVKLQLLVLTNQAVNNILVDTGRPVVSPLQSSAWTPSAGQYKDLLTILSQPEMGLINQYCQDYWQYGDPLTGVIKAKDLKELKEQKKPRNRRHHGDEEET

Target ORF information:

RefSeq Version	XM_005172909.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172909.4

ORF Insert Sequence:

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC 
CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAAACAGGA 
CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC 
CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG 
GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA 
GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG 
CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA 
AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG 
AACGATGACG GCGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT 
CTCATCTGCT CGGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT 
GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG 
TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG 
GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT 
GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG 
AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG 
TATAACTCCG TGTATCCAGT GCACAAGCGC CCTCTGGTGG TCAGGACCAA CGTGGACTAT 
GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC 
TTCCTGGGAA CAGACAAAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG 
CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA 
ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG 
CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC 
CGGGACCCCT ATTGTGCCTG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAA 
AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC 
AATTCCAACA TTAATAAGAA CACACTGGAG ACGGTGCAGT ATGGGGTGGA GGGCAGCAGC 
ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA 
GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA 
GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA 
GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC 
GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC 
TGGACCCCCA GCGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG 
GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC 
ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT 
CACGGGGACG AGGAGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fb ( XM_021472495.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328170.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa145327
Clone ID Related Accession (Same CDS sequence)	XM_021472495.1
Accession Version	XM_021472495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-3F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGGAAT ACGTGTTCCA TTTGGAGCCC 
GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC CCGTATGATC CAAAACTGAA CAGCGTCTCT 
GCTTTGATTA ATGGAGAACT GTATGCAGGG GTGTATATTG ACTTCATGGG AACAGACTCT 
GCTATTTTCC GCACTTTGGG GAAAAACGCA GTCATGCGTA CCGACCAATA CAACTCAAGA 
TGGCTGAATG ACCCATCATT TGTCCATGTG CACCTCATTC CTGACAGCGG TGAGAAAAAC 
GATGACAAAC TCTACTTTTT CTTTCGTGAA AAGTCTTCTG AAATGGGCCA GAGCCCGAAA 
TCACAATCCC GCATCGGCCG CATCTGCCTG AACGATGACG GCGGTCACTG CTGCTTGGTC 
AACAAGTGGA GCACCTTCCT GAAAGCCCGT CTCATCTGCT CGGTCCCAGG AGCTGACGGG 
ATTGAAACGC ACTTCGATGA GCTCAGAGAT GTGTACATAC AGCCCACGCA GGACACTAAA 
AACCCAGTCA TCTACGGGGT CTTCTCTGTG TCCGGCTCTG TATTCAAGGG CTCGGCGGTC 
TGCGTCTACT CCATGGCGGA CATTCGGATG GTGTTCAATG GACCTTTTGC CCACAAAGAA 
GGTCCCAATT ACCAGTGGGT GGCCTACACT GGGAAAATCC CCTACCCCCG TCCGGGAACA 
TGTCCTGGTG GGACTTTCAC CCCGAACATG AAGTCCACTA AGGACTACCC AGACGAGGTC 
ATCAACTTCA TGCGGAATCA TCCCACCATG TATAACTCCG TGTATCCAGT GCACAAGCGC 
CCTCTGGTGG TCAGGACCAA CGTGGACTAT GAGTTCACAA CCATCACTGT GGATCAGGTC 
ACAGCGGCCG ACGGAAACTA TGAGGTGCTC TTCCTGGGAA CAGACAAAGG AACAGTGCAG 
AAGGTGATCG TCCTACCCAG TGATGACCTG CAGACAGAGG AGCTTGTGCT GGAGGAAGTG 
GAGGTCTTTA AGGTCCCTAC TTCTATCACA ACAATGAAGA TTTCTTCAAA ACGGCAACAA 
CTGTATGTGG CCTCTGCGGT GGGAGTGACG CATATGGCTC TGCACCGCTG TGATGTCTAC 
GGCGAGGCCT GTGCTGACTG CTGTTTGGCC CGGGACCCCT ATTGTGCCTG GGACGGCAAA 
TCCTGCTCTA GATACTCAGC CTCGCAGAAA AGGCTTCCTT TTAGACGCAG CCGTCGACAA 
GACGTGAAAT ACGGGAACCC CATACGCCAG TGCAGAGGAT ACAATTCCAA CATTAATAAG 
AACACACTGG AGACGGTGCA GTATGGGGTG GAGGGCAGCA GCACCTTTCT GGAGTGCCAG 
GCCAGATCAC CACACGCTGC CATCAAGTGG CACCTTCACA GAGACAACAG CGACCGGAGG 
AGAGAAGTCC GCAGTGATGG CCGTGTGCTG AAGACGGATC AAGGTTTGCT TCTGCGCTCT 
CTCCAGTCCT CAGACTCCGG CATCTACATC TGCACGGCCA CAGAGAAGAA CTTCAAGCAC 
ACGCTGGTCA AACTGCAGCT GCTCGTCCTG ACCAATCAGG CCGTCAACAA CATTCTGGTG 
GACACGGGAC GTCCGGTCGT CTCTCCGCTC CAGTCCAGCG CCTGGACCCC CAGCGCCGGG 
CAATACAAAG ACCTGCTGAC CATCCTCAGC CAGCCCGAGA TGGGCCTCAT AAACCAGTAC 
TGCCAGGACT ATTGGCAGTA TGGAGACCCT CTGACCGGGG TCATCAAGGC CAAAGACCTC 
AAAGAGCTCA AGGAGCAGAA GAAACCACGA AATCGCAGAC ATCACGGGGA CGAGGAGGAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328170.1
CDS	397..2682
Translation	MLLDSLWPVQVLLVLSVLTLVLGNEPQNAPRVFLSFKDLRSTGTAHYFSFLLNTSDYRILRMDEDHDRMYVGSKDYILSLDLHDINREPLIIHWPVSPQRKTECVLSGKDTNGECGNFIRLIEPWNRTHLYICGTGAYNPVCTYVNRGRKPMEYVFHLEPGKEDSGKGKCPYDPKLNSVSALINGELYAGVYIDFMGTDSAIFRTLGKNAVMRTDQYNSRWLNDPSFVHVHLIPDSGEKNDDKLYFFFREKSSEMGQSPKSQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGADGIETHFDELRDVYIQPTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRNHPTMYNSVYPVHKRPLVVRTNVDYEFTTITVDQVTAADGNYEVLFLGTDKGTVQKVIVLPSDDLQTEELVLEEVEVFKVPTSITTMKISSKRQQLYVASAVGVTHMALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRLPFRRSRRQDVKYGNPIRQCRGYNSNINKNTLETVQYGVEGSSTFLECQARSPHAAIKWHLHRDNSDRRREVRSDGRVLKTDQGLLLRSLQSSDSGIYICTATEKNFKHTLVKLQLLVLTNQAVNNILVDTGRPVVSPLQSSAWTPSAGQYKDLLTILSQPEMGLINQYCQDYWQYGDPLTGVIKAKDLKELKEQKKPRNRRHHGDEEET

Target ORF information:

RefSeq Version	XM_021472495.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472495.1

ORF Insert Sequence:

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGGAAT ACGTGTTCCA TTTGGAGCCC 
GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC CCGTATGATC CAAAACTGAA CAGCGTCTCT 
GCTTTGATTA ATGGAGAACT GTATGCAGGG GTGTATATTG ACTTCATGGG AACAGACTCT 
GCTATTTTCC GCACTTTGGG GAAAAACGCA GTCATGCGTA CCGACCAATA CAACTCAAGA 
TGGCTGAATG ACCCATCATT TGTCCATGTG CACCTCATTC CTGACAGCGG TGAGAAAAAC 
GATGACAAAC TCTACTTTTT CTTTCGTGAA AAGTCTTCTG AAATGGGCCA GAGCCCGAAA 
TCACAATCCC GCATCGGCCG CATCTGCCTG AACGATGACG GCGGTCACTG CTGCTTGGTC 
AACAAGTGGA GCACCTTCCT GAAAGCCCGT CTCATCTGCT CGGTCCCAGG AGCTGACGGG 
ATTGAAACGC ACTTCGATGA GCTCAGAGAT GTGTACATAC AGCCCACGCA GGACACTAAA 
AACCCAGTCA TCTACGGGGT CTTCTCTGTG TCCGGCTCTG TATTCAAGGG CTCGGCGGTC 
TGCGTCTACT CCATGGCGGA CATTCGGATG GTGTTCAATG GACCTTTTGC CCACAAAGAA 
GGTCCCAATT ACCAGTGGGT GGCCTACACT GGGAAAATCC CCTACCCCCG TCCGGGAACA 
TGTCCTGGTG GGACTTTCAC CCCGAACATG AAGTCCACTA AGGACTACCC AGACGAGGTC 
ATCAACTTCA TGCGGAATCA TCCCACCATG TATAACTCCG TGTATCCAGT GCACAAGCGC 
CCTCTGGTGG TCAGGACCAA CGTGGACTAT GAGTTCACAA CCATCACTGT GGATCAGGTC 
ACAGCGGCCG ACGGAAACTA TGAGGTGCTC TTCCTGGGAA CAGACAAAGG AACAGTGCAG 
AAGGTGATCG TCCTACCCAG TGATGACCTG CAGACAGAGG AGCTTGTGCT GGAGGAAGTG 
GAGGTCTTTA AGGTCCCTAC TTCTATCACA ACAATGAAGA TTTCTTCAAA ACGGCAACAA 
CTGTATGTGG CCTCTGCGGT GGGAGTGACG CATATGGCTC TGCACCGCTG TGATGTCTAC 
GGCGAGGCCT GTGCTGACTG CTGTTTGGCC CGGGACCCCT ATTGTGCCTG GGACGGCAAA 
TCCTGCTCTA GATACTCAGC CTCGCAGAAA AGGCTTCCTT TTAGACGCAG CCGTCGACAA 
GACGTGAAAT ACGGGAACCC CATACGCCAG TGCAGAGGAT ACAATTCCAA CATTAATAAG 
AACACACTGG AGACGGTGCA GTATGGGGTG GAGGGCAGCA GCACCTTTCT GGAGTGCCAG 
GCCAGATCAC CACACGCTGC CATCAAGTGG CACCTTCACA GAGACAACAG CGACCGGAGG 
AGAGAAGTCC GCAGTGATGG CCGTGTGCTG AAGACGGATC AAGGTTTGCT TCTGCGCTCT 
CTCCAGTCCT CAGACTCCGG CATCTACATC TGCACGGCCA CAGAGAAGAA CTTCAAGCAC 
ACGCTGGTCA AACTGCAGCT GCTCGTCCTG ACCAATCAGG CCGTCAACAA CATTCTGGTG 
GACACGGGAC GTCCGGTCGT CTCTCCGCTC CAGTCCAGCG CCTGGACCCC CAGCGCCGGG 
CAATACAAAG ACCTGCTGAC CATCCTCAGC CAGCCCGAGA TGGGCCTCAT AAACCAGTAC 
TGCCAGGACT ATTGGCAGTA TGGAGACCCT CTGACCGGGG TCATCAAGGC CAAAGACCTC 
AAAGAGCTCA AGGAGCAGAA GAAACCACGA AATCGCAGAC ATCACGGGGA CGAGGAGGAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fb ( XM_021472496.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328171.1 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X4, mRNA.

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CloneID	ODa145328
Clone ID Related Accession (Same CDS sequence)	XM_021472496.1
Accession Version	XM_021472496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-3F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGGAAT ACGTGTTCCA TTTGGAGCCC 
GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC CCGTATGATC CAAAACTGAA CAGCGTCTCT 
GCTTTGATTA ATGGAGAACT GTATGCAGGG GTGTATATTG ACTTCATGGG AACAGACTCT 
GCTATTTTCC GCACTTTGGG GAAAAACGCA GTCATGCGTA CCGACCAATA CAACTCAAGA 
TGGCTGAATG ACCCATCATT TGTCCATGTG CACCTCATTC CTGACAGCGG TGAGAAAAAC 
GATGACAAAC TCTACTTTTT CTTTCGTGAA AAGTCTTCTG AAATGGGCCA GAGCCCGAAA 
TCACAATCCC GCATCGGCCG CATCTGCCTG AACGATGACG GCGGTCACTG CTGCTTGGTC 
AACAAGTGGA GCACCTTCCT GAAAGCCCGT CTCATCTGCT CGGTCCCAGG AGCTGACGGG 
ATTGAAACGC ACTTCGATGA GCTCAGAGAT GTGTACATAC AGCCCACGCA GGACACTAAA 
AACCCAGTCA TCTACGGGGT CTTCTCTGTG TCCGGCTCTG TATTCAAGGG CTCGGCGGTC 
TGCGTCTACT CCATGGCGGA CATTCGGATG GTGTTCAATG GACCTTTTGC CCACAAAGAA 
GGTCCCAATT ACCAGTGGGT GGCCTACACT GGGAAAATCC CCTACCCCCG TCCGGGAACA 
TGTCCTGGTG GGACTTTCAC CCCGAACATG AAGTCCACTA AGGACTACCC AGACGAGGTC 
ATCAACTTCA TGCGGAATCA TCCCACCATG TATAACTCCG TGTATCCAGT GCACAAGCGC 
CCTCTGGTGG TCAGGACCAA CGTGGACTAT GAGTTCACAA CCATCACTGT GGATCAGGTC 
ACAGCGGCCG ACGGAAACTA TGAGGTGCTC TTCCTGGGAA CAGACAAAGG AACAGTGCAG 
AAGGTGATCG TCCTACCCAG TGATGACCTG CAGACAGAGG AGCTTGTGCT GGAGGAAGTG 
GAGGTCTTTA AGGTCCCTAC TTCTATCACA ACAATGAAGA TTTCTTCAAA ACGGCAACAA 
CTGTATGTGG CCTCTGCGGT GGGAGTGACG CATATGGCTC TGCACCGCTG TGATGTCTAC 
GGCGAGGCCT GTGCTGACTG CTGTTTGGCC CGGGACCCCT ATTGTGCCTG GGACGGCAAA 
TCCTGCTCTA GATACTCAGC CTCGCAGAAA AGACGCAGCC GTCGACAAGA CGTGAAATAC 
GGGAACCCCA TACGCCAGTG CAGAGGATAC AATTCCAACA TTAATAAGAA CACACTGGAG 
ACGGTGCAGT ATGGGGTGGA GGGCAGCAGC ACCTTTCTGG AGTGCCAGGC CAGATCACCA 
CACGCTGCCA TCAAGTGGCA CCTTCACAGA GACAACAGCG ACCGGAGGAG AGAAGTCCGC 
AGTGATGGCC GTGTGCTGAA GACGGATCAA GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA 
GACTCCGGCA TCTACATCTG CACGGCCACA GAGAAGAACT TCAAGCACAC GCTGGTCAAA 
CTGCAGCTGC TCGTCCTGAC CAATCAGGCC GTCAACAACA TTCTGGTGGA CACGGGACGT 
CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC TGGACCCCCA GCGCCGGGCA ATACAAAGAC 
CTGCTGACCA TCCTCAGCCA GCCCGAGATG GGCCTCATAA ACCAGTACTG CCAGGACTAT 
TGGCAGTATG GAGACCCTCT GACCGGGGTC ATCAAGGCCA AAGACCTCAA AGAGCTCAAG 
GAGCAGAAGA AACCACGAAA TCGCAGACAT CACGGGGACG AGGAGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328171.1
CDS	397..2670
Translation	MLLDSLWPVQVLLVLSVLTLVLGNEPQNAPRVFLSFKDLRSTGTAHYFSFLLNTSDYRILRMDEDHDRMYVGSKDYILSLDLHDINREPLIIHWPVSPQRKTECVLSGKDTNGECGNFIRLIEPWNRTHLYICGTGAYNPVCTYVNRGRKPMEYVFHLEPGKEDSGKGKCPYDPKLNSVSALINGELYAGVYIDFMGTDSAIFRTLGKNAVMRTDQYNSRWLNDPSFVHVHLIPDSGEKNDDKLYFFFREKSSEMGQSPKSQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGADGIETHFDELRDVYIQPTQDTKNPVIYGVFSVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPNMKSTKDYPDEVINFMRNHPTMYNSVYPVHKRPLVVRTNVDYEFTTITVDQVTAADGNYEVLFLGTDKGTVQKVIVLPSDDLQTEELVLEEVEVFKVPTSITTMKISSKRQQLYVASAVGVTHMALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRRSRRQDVKYGNPIRQCRGYNSNINKNTLETVQYGVEGSSTFLECQARSPHAAIKWHLHRDNSDRRREVRSDGRVLKTDQGLLLRSLQSSDSGIYICTATEKNFKHTLVKLQLLVLTNQAVNNILVDTGRPVVSPLQSSAWTPSAGQYKDLLTILSQPEMGLINQYCQDYWQYGDPLTGVIKAKDLKELKEQKKPRNRRHHGDEEET

Target ORF information:

RefSeq Version	XM_021472496.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472496.1

ORF Insert Sequence:

ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG 
TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG 
CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG 
GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG 
AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT 
CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT 
GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGGAAT ACGTGTTCCA TTTGGAGCCC 
GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC CCGTATGATC CAAAACTGAA CAGCGTCTCT 
GCTTTGATTA ATGGAGAACT GTATGCAGGG GTGTATATTG ACTTCATGGG AACAGACTCT 
GCTATTTTCC GCACTTTGGG GAAAAACGCA GTCATGCGTA CCGACCAATA CAACTCAAGA 
TGGCTGAATG ACCCATCATT TGTCCATGTG CACCTCATTC CTGACAGCGG TGAGAAAAAC 
GATGACAAAC TCTACTTTTT CTTTCGTGAA AAGTCTTCTG AAATGGGCCA GAGCCCGAAA 
TCACAATCCC GCATCGGCCG CATCTGCCTG AACGATGACG GCGGTCACTG CTGCTTGGTC 
AACAAGTGGA GCACCTTCCT GAAAGCCCGT CTCATCTGCT CGGTCCCAGG AGCTGACGGG 
ATTGAAACGC ACTTCGATGA GCTCAGAGAT GTGTACATAC AGCCCACGCA GGACACTAAA 
AACCCAGTCA TCTACGGGGT CTTCTCTGTG TCCGGCTCTG TATTCAAGGG CTCGGCGGTC 
TGCGTCTACT CCATGGCGGA CATTCGGATG GTGTTCAATG GACCTTTTGC CCACAAAGAA 
GGTCCCAATT ACCAGTGGGT GGCCTACACT GGGAAAATCC CCTACCCCCG TCCGGGAACA 
TGTCCTGGTG GGACTTTCAC CCCGAACATG AAGTCCACTA AGGACTACCC AGACGAGGTC 
ATCAACTTCA TGCGGAATCA TCCCACCATG TATAACTCCG TGTATCCAGT GCACAAGCGC 
CCTCTGGTGG TCAGGACCAA CGTGGACTAT GAGTTCACAA CCATCACTGT GGATCAGGTC 
ACAGCGGCCG ACGGAAACTA TGAGGTGCTC TTCCTGGGAA CAGACAAAGG AACAGTGCAG 
AAGGTGATCG TCCTACCCAG TGATGACCTG CAGACAGAGG AGCTTGTGCT GGAGGAAGTG 
GAGGTCTTTA AGGTCCCTAC TTCTATCACA ACAATGAAGA TTTCTTCAAA ACGGCAACAA 
CTGTATGTGG CCTCTGCGGT GGGAGTGACG CATATGGCTC TGCACCGCTG TGATGTCTAC 
GGCGAGGCCT GTGCTGACTG CTGTTTGGCC CGGGACCCCT ATTGTGCCTG GGACGGCAAA 
TCCTGCTCTA GATACTCAGC CTCGCAGAAA AGACGCAGCC GTCGACAAGA CGTGAAATAC 
GGGAACCCCA TACGCCAGTG CAGAGGATAC AATTCCAACA TTAATAAGAA CACACTGGAG 
ACGGTGCAGT ATGGGGTGGA GGGCAGCAGC ACCTTTCTGG AGTGCCAGGC CAGATCACCA 
CACGCTGCCA TCAAGTGGCA CCTTCACAGA GACAACAGCG ACCGGAGGAG AGAAGTCCGC 
AGTGATGGCC GTGTGCTGAA GACGGATCAA GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA 
GACTCCGGCA TCTACATCTG CACGGCCACA GAGAAGAACT TCAAGCACAC GCTGGTCAAA 
CTGCAGCTGC TCGTCCTGAC CAATCAGGCC GTCAACAACA TTCTGGTGGA CACGGGACGT 
CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC TGGACCCCCA GCGCCGGGCA ATACAAAGAC 
CTGCTGACCA TCCTCAGCCA GCCCGAGATG GGCCTCATAA ACCAGTACTG CCAGGACTAT 
TGGCAGTATG GAGACCCTCT GACCGGGGTC ATCAAGGCCA AAGACCTCAA AGAGCTCAAG 
GAGCAGAAGA AACCACGAAA TCGCAGACAT CACGGGGACG AGGAGGAGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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sema3fb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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