TLN2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024997997.1	XP_024853765.1	talin-2 isoform X1
XM_024997999.1	XP_024853767.1	talin-2 isoform X2
XM_024998002.1	XP_024853770.1	talin-2 isoform X4
XM_024998003.1	XP_024853771.1	talin-2 isoform X5
XM_015473169.1	XP_015328655.1	LOW QUALITY PROTEIN: talin-2
XM_024997996.1	XP_024853764.1	talin-2 isoform X1
XM_024998000.1	XP_024853768.1	talin-2 isoform X3
XM_024998001.1	XP_024853769.1	talin-2 isoform X4
XM_015465076.1	XP_015320562.1	LOW QUALITY PROTEIN: talin-2
XM_024997995.1	XP_024853763.1	talin-2 isoform X1
XM_024997998.1	XP_024853766.1	talin-2 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa06524	XM_024998002.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa06524	XM_024998001.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa219848	XM_024997995.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa219848	XM_024997997.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa219848	XM_024997998.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa219848	XM_024997996.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa219849	XM_024998000.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa219850	XM_024998003.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa51531	XM_024997999.1 Latest version!	Bos taurus talin 2 (TLN2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa23286	XM_015473169.1 Latest version!	Bos taurus talin 2 (TLN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa23286	XM_015465076.1 Latest version!	Bos taurus talin 2 (TLN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

TLN2 ( XM_024998002.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853770.1 Bos taurus talin 2 (TLN2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa06524
Clone ID Related Accession (Same CDS sequence)	XM_024998002.1 , XM_024998001.1
Accession Version	XM_024998002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGCAGCA 
GGAAATGCTG TGAAAAGAGC CTCAGACAAC CTTGTTCGTG CAGCCCAGAA GGCAGCGTTT 
GGCAAAGCTG ATGATGACGA TGTTGTAGTG AAAACAAAGT TTGTAGGGGG CATTGCTCAG 
ATCATCGCCG CCCAGGAGGA AATGCTGAAG AAGGAACGAG AATTGGAAGA AGCAAGGAAA 
AAACTGGCCC AGATCCGCCA ACAGCAGTAT AAATTCCTAC CCACTGAGCT GAGAGAGGAC 
GAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853770.1
CDS	366..7994
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024998002.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998002.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGCAGCA 
GGAAATGCTG TGAAAAGAGC CTCAGACAAC CTTGTTCGTG CAGCCCAGAA GGCAGCGTTT 
GGCAAAGCTG ATGATGACGA TGTTGTAGTG AAAACAAAGT TTGTAGGGGG CATTGCTCAG 
ATCATCGCCG CCCAGGAGGA AATGCTGAAG AAGGAACGAG AATTGGAAGA AGCAAGGAAA 
AAACTGGCCC AGATCCGCCA ACAGCAGTAT AAATTCCTAC CCACTGAGCT GAGAGAGGAC 
GAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_024998001.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853769.1 Bos taurus talin 2 (TLN2), transcript variant X7, mRNA.

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CloneID	OBa06524
Clone ID Related Accession (Same CDS sequence)	XM_024998002.1 , XM_024998001.1
Accession Version	XM_024998001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGCAGCA 
GGAAATGCTG TGAAAAGAGC CTCAGACAAC CTTGTTCGTG CAGCCCAGAA GGCAGCGTTT 
GGCAAAGCTG ATGATGACGA TGTTGTAGTG AAAACAAAGT TTGTAGGGGG CATTGCTCAG 
ATCATCGCCG CCCAGGAGGA AATGCTGAAG AAGGAACGAG AATTGGAAGA AGCAAGGAAA 
AAACTGGCCC AGATCCGCCA ACAGCAGTAT AAATTCCTAC CCACTGAGCT GAGAGAGGAC 
GAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853769.1
CDS	328..7956
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024998001.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998001.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGCAGCA 
GGAAATGCTG TGAAAAGAGC CTCAGACAAC CTTGTTCGTG CAGCCCAGAA GGCAGCGTTT 
GGCAAAGCTG ATGATGACGA TGTTGTAGTG AAAACAAAGT TTGTAGGGGG CATTGCTCAG 
ATCATCGCCG CCCAGGAGGA AATGCTGAAG AAGGAACGAG AATTGGAAGA AGCAAGGAAA 
AAACTGGCCC AGATCCGCCA ACAGCAGTAT AAATTCCTAC CCACTGAGCT GAGAGAGGAC 
GAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_024997997.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853765.1 Bos taurus talin 2 (TLN2), transcript variant X3, mRNA.

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CloneID	OBa219848
Clone ID Related Accession (Same CDS sequence)	XM_024997997.1 , XM_024997996.1 , XM_024997995.1 , XM_024997998.1
Accession Version	XM_024997997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853765.1
CDS	264..7970
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGETRLHDALRPGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQVMVTDAGGKILLLERAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024997997.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997997.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_024997996.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853764.1 Bos taurus talin 2 (TLN2), transcript variant X2, mRNA.

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CloneID	OBa219848
Clone ID Related Accession (Same CDS sequence)	XM_024997997.1 , XM_024997996.1 , XM_024997995.1 , XM_024997998.1
Accession Version	XM_024997996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853764.1
CDS	481..8187
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGETRLHDALRPGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQVMVTDAGGKILLLERAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024997996.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997996.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_024997995.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853763.1 Bos taurus talin 2 (TLN2), transcript variant X1, mRNA.

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CloneID	OBa219848
Clone ID Related Accession (Same CDS sequence)	XM_024997997.1 , XM_024997996.1 , XM_024997995.1 , XM_024997998.1
Accession Version	XM_024997995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853763.1
CDS	328..8034
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGETRLHDALRPGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQVMVTDAGGKILLLERAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024997995.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997995.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_024997998.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853766.1 Bos taurus talin 2 (TLN2), transcript variant X4, mRNA.

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CloneID	OBa219848
Clone ID Related Accession (Same CDS sequence)	XM_024997997.1 , XM_024997996.1 , XM_024997995.1 , XM_024997998.1
Accession Version	XM_024997998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853766.1
CDS	263..7969
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGETRLHDALRPGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQVMVTDAGGKILLLERAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024997998.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997998.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_024998000.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853768.1 Bos taurus talin 2 (TLN2), transcript variant X6, mRNA.

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CloneID	OBa219849
Clone ID Related Accession (Same CDS sequence)	XM_024998000.1
Accession Version	XM_024998000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGCA GCAGGAAATG CTGTGAAAAG AGCCTCAGAC 
AACCTTGTTC GTGCAGCCCA GAAGGCAGCG TTTGGCAAAG CTGATGATGA CGATGTTGTA 
GTGAAAACAA AGTTTGTAGG GGGCATTGCT CAGATCATCG CCGCCCAGGA GGAAATGCTG 
AAGAAGGAAC GAGAATTGGA AGAAGCAAGG AAAAAACTGG CCCAGATCCG CCAACAGCAG 
TATAAATTCC TACCCACTGA GCTGAGAGAG GACGAAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853768.1
CDS	328..7989
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGETRLHDALRPGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024998000.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998000.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TGACTAAGTC GGTTACTAAC CCGGAGGAGT TGGGAGGACT GGCTTCACAA 
ATGACCAGTG ACTATGGGCA CCTGGCTCTC CAGGGCCAGC TGGCAGCAGC CACGGCGGAA 
CCAGAGGAGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGCA GCAGGAAATG CTGTGAAAAG AGCCTCAGAC 
AACCTTGTTC GTGCAGCCCA GAAGGCAGCG TTTGGCAAAG CTGATGATGA CGATGTTGTA 
GTGAAAACAA AGTTTGTAGG GGGCATTGCT CAGATCATCG CCGCCCAGGA GGAAATGCTG 
AAGAAGGAAC GAGAATTGGA AGAAGCAAGG AAAAAACTGG CCCAGATCCG CCAACAGCAG 
TATAAATTCC TACCCACTGA GCTGAGAGAG GACGAAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_024998003.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853771.1 Bos taurus talin 2 (TLN2), transcript variant X9, mRNA.

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CloneID	OBa219850
Clone ID Related Accession (Same CDS sequence)	XM_024998003.1
Accession Version	XM_024998003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853771.1
CDS	328..7914
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGETRLHDALRPGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQVMVTDAGGKILLLERAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024998003.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998003.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGT 
GAGACTCGCC TTCATGATGC CCTGAGGCCA GGGGTGGCTG CTCGAGAGAC GGCCCAAGCT 
CTGAAAACGC TGGCCCAGGC TGCCCGGGGC GTGGCTGCAT CCACGAGTGA CCCGGCGGCT 
GCGCGCGCCA TGTTGGATTC TGCCCGGGAC GTGATGGAGG GCTCGGCCAT GCTCATCCAG 
GAGGCCAAAC AGGCCCTGAT TGCACCCGGA GACGCCGAGA GTCAGCAGAG ACTAGCGCAG 
GTGGCCAAAG CCGTTTCTCA CTCATTGAAT AACTGTGTGA ACTGTCTCCC TGGGCAGAAG 
GATGTGGATG TGGCCTTGAA GAGCATTGGG GAGTCAAGCA AGAAGCTGCT GGTGGACTCG 
TTACCGCCAA GCACAAAGCC CTTCCAGGAA GCCCAGAGTG AACTGAACCA AGCAGCCGCC 
GATCTGAACC AGTCTGCGGG GGATGTGGTC CACGCCACCC GGGGCCAGAG TGGAGAGCTG 
GCCGCAGCCT CCGGGAAGTT CAGTGATGAC TTCGATGAGT TCCTCGATGC TGGCATTGAG 
ATGGCAGGCC AGGCGCAGAC AAAAGAAGAC CAGATCCAAC TCATAGGGAA CCTCAAGAAT 
ATCTCCATGG CCTCCAGCAA GCTATTGTTA GCTGCCAAAT CTCTCTCTGT AGACCCAGGA 
GCCCCCAATG CGAAAAATCT CCTGGCTGCA GCTGCAAGAG CTGTGACTGA GAGCATCAAC 
CAGCTCATCA CGCTGTGCAC GCAACAAGCT CCTGGCCAGA AGGAATGTGA TAACGCCCTG 
CGGGAACTCG AGACTGTCAA GGGGATGTTG GACAATCCCA ATGAACCTGT CAGTGACCTC 
TCGTACTTTG ACTGCATCGA GAGTGTGATG GAAAACTCCA AGGTTCTGGG AGAATCGATG 
GCAGGGATTT CGCAGAATGC CAAAACCGGG GACCTCCCTG CCTTTGGGGA GTGTGTGGGG 
ATTGCGTCCA AGGCTCTCTG TGGGCTGACG GAGGCTGCAG CCCAGGCTGC TTACCTGGTC 
GGCGTCTCTG ATCCAAACAG CCAGGCAGGC CACCAGGGCC TTGTGGATCC CATCCAGTTT 
GCCAGGGCCA ACCAGGCTAT CCAGATGGCG TGCCAGAACC TGGTGGATCC CGGCAGCAGC 
CCGTCACAGG TTTTGTCTGC TGCCACGATT GTCGCCAAGC ACACCTCAGC CCTGTGCAAC 
GCCTGCCGAA TCGCCTCATC CAAGACGGGC AACCCCGTGG CCAAGAGGCA CTTTGTCCAG 
TCCGCCAAGG AGGTCGCCAA CAGCACCGCC AACCTGGTGA AGACCATAAA GGCCCTGGAT 
GGGGACTTCT CCGAGGACAA CCGCAACAAG TGTCGCGTCG CCACTGCCCC CTTGATCGCA 
GCCGTGGAGA ACTTGACGGC ATTCGCCTCC AACCCTGAGT TTGTCAGTGT TCCCGCCCAG 
ATCAGCTCCG AGGGCTCCCA GGCACAGGAA CCAATCCTGG TCTCAGCCAA GACTATGCTG 
GAAAGCTCAT CGTACCTCAT CCGCACTGCC CGCTCACTGG CCATCAACCC CAAAGACCCG 
CCCACCTGGT CTGTGCTAGC CGGACATTCC CACACTGTGT CTGACTCTAT CAAGAGTCTC 
ATCACGTCCA TCAGGGACAA GGCCCCGGGA CAGAGAGAGT GTGACTTCTC CATCGACAGC 
ATCAACCGGT GCATCCGGGA CATCGAGCAG GCCTCGTTGG CGGCCGTCAG CCAGAGCTTG 
GCCACGAGGG ATGACATCTC CGTGGAGGCC TTACAGGAGC AGCTGACTTC AGTGGTCCAG 
GAAATCGGGC ACCTTATCGA TCCCATTGCC ACAGCGGCTC GGGGAGAAGC AGCTCAGCTG 
GGACATAAGG TGACACAGCT GGCGAGCTAC TTTGAGCCCT TGATCTTAGC TGCAGTGGGT 
GTCGCCTCCA AGATTCTGGA CCATCAGCAG CAGATGACGG TGCTGGACCA GACCAAGACG 
CTCGCAGAGT CTGCCCTGCA GATGCTGTAT GCAGCCAAGG AAGGCGGAGG AAACCCCAAG 
GCACAGCACA CCCACGATGC CATCACGGAG GCCGCCCAGC TCATGAAAGA GGCCGTGGAC 
GACATCATGG TGACACTGAA TGAAGCTGCC AGTGAAGTGG GGTTGGTGGG AGGTATGGTT 
GACGCCATCG CAGAAGCCAT GAGCAAGCTG GATGAAAGTA CTCCTCCAGA ACCGAAGGGG 
ACGTTTGTCG ACTATCAGAC GACGGTGGTT AAATACTCCA AAGCTATCGC GGTAACAGCT 
CAGGAAATGA TCGGCTTCCA GATTCGCACT CGCGTACAGG ACCTGGGCCA CGGCTGCATC 
TTCCTGGTGC AGAAGGCGGG GGCCCTCCAG GTGTGCCCCA CGGACAGCTA CACCAAGAGG 
GAGCTGATAG AGTGTGCCCG CGCTGTCACA GAGAAGGTAT CCTTGGTGCT GTCCGCTCTC 
CAGGCTGGGA ACAAGGGGAC CCAGGCATGT ATCACAGCTG CCACTGCCGT ATCTGGGATC 
ATTGCCGACC TGGACACCAC CATCATGTTT GCGACTGCAG GGACCTTGAA TGCAGAGAAC 
AACGAGACAT TCGCAGACCA CAGGGAGAAC ATTCTGAAGA CAGCCAAGGC CTTGGTGGAA 
GACACAAAAT TGCTGGTGTC GGGGGCTGCG TCCACTCCAG ACAAGCTGGC CCAGGCAGCT 
CAGTCCTCTG CAGCCACCAT CACCCAGCTC GCTGAGGTGG TCAAGCTGGG GGCCGCCAGC 
TTGGGCTCTG ATGACCCCGA GACCCAGGTA GTGTTGATCA ATGCCATCAA AGACGTGGCC 
AAGGCCCTCT CAGATCTCAT TGGTGCTACC AAGGGAGCTG CCAGCAAGCC TGCCGATGAC 
CCCTCCATGT ACCAGCTCAA GGGGGCTGCC AAGGTGATGG TGACCAATGT CACCTCCCTC 
CTCAAGACCG TGAAGGCAGT GGAGGACGAG GCCACTCGGG GCACACGGGC ACTAGAGGCC 
ACCATCGAGT ACATGAAGCA GGAGCTCACG GTATTCCAGT CAAAAGAGGT ACCTGAGAAG 
ACATCATCAC CTGAAGAATC CATCAGGATG ACCAAAGGCA TCACCATGGC AACGGCCAAA 
GCTGTGGCTG CTGGGAACTC TTGTCGACAG GAGGACGTGA TAGCCACTGC CAATCTGAGC 
CGGAAGGCCG TGTCAGACAT GTTGACGGCC TGCAAGCAAG CTTCCTTCCA CCCTGACGTC 
AGTGAGGAGG TGCGCACCAG AGCCTTGCGG TATGGGACAG AGTGCACCCT CGGCTACCTG 
GACCTCCTGG AGCACGTCTT GGTGATTCTT CAGAAACCAA CACCAGAACT TAAGCACCAG 
CTGGCTGCTT TCTCCAAGCG CGTGGCTGGC GCCGTGACCG AGCTCATCCA GGCAGCAGAG 
GCCATGAAAG GAACAGAGTG GGTGGATCCC GAAGACCCAA CCGTCATTGC AGAAACAGAA 
TTACTGGGGG CTGCAGCATC CATTGAGGCT GCTGCTAAGA AGTTAGAGCA GCTGAAGCCA 
AGAGCAAAAC CGAAACAAGC GGACGAGACC CTGGATTTTG AAGAACAGAT CTTGGAGGCT 
GCTAAATCCA TTGCTGCTGC CACGAGTGCC CTGGTCAAAT CGGCCTCGGC AGCCCAGAGG 
GAGCTGGTGG CCCAAGGAAA GGTGGGTTCC ATTCCTGCCA ATGCTGCGGA CGACGGACAG 
TGGTCGCAGG GCCTGATTTC TGCCGCCCGG ATGGTGGCAG CCGCGACCAG CAGTCTCTGC 
GAGGCAGCCA ACGCCTCAGT GCAGGGACAC GCCAGCGAGG AGAAGCTCAT CTCATCTGCC 
AAGCAGGTAG CTGCATCCAC GGCTCAGCTG CTTGTGGCCT GCAAGGTGAA GGCTGATCAG 
GATTCGGAGG CCATGAGGCG GCTACAGGTA ATGGTCACTG ATGCTGGTGG GAAAATACTC 
CTGTTGGAGC GGGCAGCAGG AAATGCTGTG AAAAGAGCCT CAGACAACCT TGTTCGTGCA 
GCCCAGAAGG CAGCGTTTGG CAAAGCTGAT GATGACGATG TTGTAGTGAA AACAAAGTTT 
GTAGGGGGCA TTGCTCAGAT CATCGCCGCC CAGGAGGAAA TGCTGAAGAA GGAACGAGAA 
TTGGAAGAAG CAAGGAAAAA ACTGGCCCAG ATCCGCCAAC AGCAGTATAA ATTCCTACCC 
ACTGAGCTGA GAGAGGACGA AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_024997999.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853767.1 Bos taurus talin 2 (TLN2), transcript variant X5, mRNA.

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CloneID	OBa51531
Clone ID Related Accession (Same CDS sequence)	XM_024997999.1
Accession Version	XM_024997999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	talin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGTAATG 
GTCACTGATG CTGGTGGGAA AATACTCCTG TTGGAGCGGG CAGCAGGAAA TGCTGTGAAA 
AGAGCCTCAG ACAACCTTGT TCGTGCAGCC CAGAAGGCAG CGTTTGGCAA AGCTGATGAT 
GACGATGTTG TAGTGAAAAC AAAGTTTGTA GGGGGCATTG CTCAGATCAT CGCCGCCCAG 
GAGGAAATGC TGAAGAAGGA ACGAGAATTG GAAGAAGCAA GGAAAAAACT GGCCCAGATC 
CGCCAACAGC AGTATAAATT CCTACCCACT GAGCTGAGAG AGGACGAAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853767.1
CDS	328..8001
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQVMVTDAGGKILLLERAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_024997999.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997999.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCGCCG CTGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGTAATG 
GTCACTGATG CTGGTGGGAA AATACTCCTG TTGGAGCGGG CAGCAGGAAA TGCTGTGAAA 
AGAGCCTCAG ACAACCTTGT TCGTGCAGCC CAGAAGGCAG CGTTTGGCAA AGCTGATGAT 
GACGATGTTG TAGTGAAAAC AAAGTTTGTA GGGGGCATTG CTCAGATCAT CGCCGCCCAG 
GAGGAAATGC TGAAGAAGGA ACGAGAATTG GAAGAAGCAA GGAAAAAACT GGCCCAGATC 
CGCCAACAGC AGTATAAATT CCTACCCACT GAGCTGAGAG AGGACGAAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_015473169.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328655.1 Bos taurus talin 2 (TLN2), mRNA.

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CloneID	OBa23286
Clone ID Related Accession (Same CDS sequence)	XM_015473169.1 , XM_015465076.1
Accession Version	XM_015473169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: talin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCAAAA AAGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGCAGCA 
GGAAATGCTG TGAAAAGAGC CTCAGACAAC CTTGTTCGTG CAGCCCAGAA GGCAGCGTTT 
GGCAAAGCTG ATGATGACGA TGTTGTAGTG AAAACAAAGT TTGTAGGGGG CATTGCTCAG 
ATCATCGCCG CCCAGGAGGA AATGCTGAAG AAGGAACGAG AATTGGAAGA AGCAAGGAAA 
AAACTGGCCC AGATCCGCCA ACAGCAGTAT AAATTCCTAC CCACTGAGCT GAGAGAGGAC 
GAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328655.1
CDS	405..8033
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTKKGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_015473169.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473169.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCAAAA AAGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGCAGCA 
GGAAATGCTG TGAAAAGAGC CTCAGACAAC CTTGTTCGTG CAGCCCAGAA GGCAGCGTTT 
GGCAAAGCTG ATGATGACGA TGTTGTAGTG AAAACAAAGT TTGTAGGGGG CATTGCTCAG 
ATCATCGCCG CCCAGGAGGA AATGCTGAAG AAGGAACGAG AATTGGAAGA AGCAAGGAAA 
AAACTGGCCC AGATCCGCCA ACAGCAGTAT AAATTCCTAC CCACTGAGCT GAGAGAGGAC 
GAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2 ( XM_015465076.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320562.1 Bos taurus talin 2 (TLN2), mRNA.

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CloneID	OBa23286
Clone ID Related Accession (Same CDS sequence)	XM_015473169.1 , XM_015465076.1
Accession Version	XM_015465076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: talin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCAAAA AAGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGCAGCA 
GGAAATGCTG TGAAAAGAGC CTCAGACAAC CTTGTTCGTG CAGCCCAGAA GGCAGCGTTT 
GGCAAAGCTG ATGATGACGA TGTTGTAGTG AAAACAAAGT TTGTAGGGGG CATTGCTCAG 
ATCATCGCCG CCCAGGAGGA AATGCTGAAG AAGGAACGAG AATTGGAAGA AGCAAGGAAA 
AAACTGGCCC AGATCCGCCA ACAGCAGTAT AAATTCCTAC CCACTGAGCT GAGAGAGGAC 
GAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320562.1
CDS	405..8033
Translation	MVALSLKICVRHCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASDYGLFLSDEDPRKGIWLEAGRTLDYYMLRNGDILEYKKKQRPQKIRMLDGSVKTVMVDDSKTVGELLVTICSRIGITNYEEYSLIQETIEEKKEEGTGTLKKDRTLLRDERKMEKLKAKLHTDDDLNWLDHSRTFREQGVDENETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFEKACEFGGFQAQIQFGPHVEHKHKPGFLDLKEFLPKEYIKQRGAEKRIFQEHKNCGEMSEIEAKVKYVKLARSLRTYGVSFFLVKEKMKGKNKLVPRLLGITKDSVMRVDEKTKEVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKVEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRGHMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAGTASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDEVGSGEDLLRAARTLAGAVSDLLKAVQPTSGEPRQTVLTKKGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVAEDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATDDTELLKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNAAAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAVSNKNPAAQQQLVQSCKAVADHIPQLVQGVRGSQAQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEACGPMEIDSALSTVQTLKSELQDAKMAAVESQLKPLPGETLEKCAQDLGSTSKAVGSSMAQLLTCAAQGNEHYTGVAARETAQALKTLAQAARGVAASTSDPAAARAMLDSARDVMEGSAMLIQEAKQALIAPGDAESQQRLAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGESSKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAGDVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQIQLIGNLKNISMASSKLLLAAKSLSVDPGAPNAKNLLAAAARAVTESINQLITLCTQQAPGQKECDNALRELETVKGMLDNPNEPVSDLSYFDCIESVMENSKVLGESMAGISQNAKTGDLPAFGECVGIASKALCGLTEAAAQAAYLVGVSDPNSQAGHQGLVDPIQFARANQAIQMACQNLVDPGSSPSQVLSAATIVAKHTSALCNACRIASSKTGNPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEDNRNKCRVATAPLIAAVENLTAFASNPEFVSVPAQISSEGSQAQEPILVSAKTMLESSSYLIRTARSLAINPKDPPTWSVLAGHSHTVSDSIKSLITSIRDKAPGQRECDFSIDSINRCIRDIEQASLAAVSQSLATRDDISVEALQEQLTSVVQEIGHLIDPIATAARGEAAQLGHKVTQLASYFEPLILAAVGVASKILDHQQQMTVLDQTKTLAESALQMLYAAKEGGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAIAEAMSKLDESTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTSDYGHLALQGQLAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARAVTEKVSLVLSALQAGNKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENNETFADHRENILKTAKALVEDTKLLVSGAASTPDKLAQAAQSSAATITQLAEVVKLGAASLGSDDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSMYQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYMKQELTVFQSKEVPEKTSSPEESIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLTACKQASFHPDVSEEVRTRALRYGTECTLGYLDLLEHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQGLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEEARKKLAQIRQQQYKFLPTELREDEG

Target ORF information:

RefSeq Version	XM_015465076.1
Organism	Bos taurus(cattle)
Definition	Bos taurus talin 2 (TLN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465076.1

ORF Insert Sequence:

ATGGTGGCTC TGTCTTTAAA GATTTGTGTC CGCCACTGCA ATGTGGTCAA GACCATGCAG 
TTTGAGCCAT CAACGGCTGT GTACGATGCG TGTCGAGTCA TTCGAGAAAG GGTACCCGAG 
GCGCAAACTG GCCAAGCGTC TGACTATGGG CTATTTCTCT CGGATGAAGA CCCCAGGAAA 
GGAATTTGGC TGGAAGCAGG CAGAACACTG GATTACTACA TGTTGAGAAA TGGGGATATT 
TTGGAATATA AAAAGAAACA GAGACCTCAG AAAATTCGGA TGCTTGATGG ATCTGTGAAA 
ACGGTGATGG TGGACGACTC CAAGACAGTC GGGGAACTCC TGGTTACTAT CTGCAGCAGG 
ATAGGAATTA CAAACTATGA AGAATACTCT TTAATCCAAG AAACAATTGA AGAAAAGAAA 
GAAGAAGGGA CGGGTACGCT AAAAAAAGAC AGGACACTGT TAAGAGATGA GAGGAAGATG 
GAGAAGCTGA AGGCCAAGCT TCACACGGAT GACGACTTAA ATTGGCTGGA TCACAGTCGA 
ACATTCAGAG AACAAGGAGT AGATGAAAAT GAAACACTGC TGCTTAGACG GAAGTTCTTT 
TACTCTGATC AGAATGTGGA CTCAAGAGAC CCCGTGCAGC TGAACTTGCT CTATGTTCAG 
GCTCGGGATG ATATCCTGAA TGGCTCCCAC CCTGTCTCCT TCGAGAAAGC ATGTGAGTTT 
GGTGGATTTC AAGCCCAGAT TCAATTCGGA CCTCATGTGG AGCATAAACA CAAGCCTGGA 
TTCTTAGATC TAAAAGAATT CCTGCCCAAA GAATACATCA AGCAGAGAGG AGCTGAAAAG 
AGAATATTTC AGGAGCACAA GAACTGTGGA GAGATGAGCG AAATAGAAGC CAAAGTCAAG 
TATGTCAAAT TGGCGCGGTC CCTTCGGACA TATGGTGTGT CCTTCTTCCT GGTGAAGGAA 
AAGATGAAAG GGAAGAACAA GCTGGTGCCT CGCCTGCTGG GGATCACCAA AGACTCAGTG 
ATGCGTGTGG ATGAAAAGAC CAAGGAAGTG TTGCAGGAGT GGCCACTGAC CACGGTCAAG 
CGCTGGGCAG CCTCACCCAA GAGCTTCACA CTGGATTTTG GGGAGTATCA GGAAAGCTAC 
TACTCCGTAC AGACCACTGA AGGAGAGCAA ATATCCCAGC TGATTGCAGG CTACATTGAC 
ATCATCCTCA AAAAGAAACA AAGTAAAGAT AGATTTGGAC TGGAGGGCGA TGAGGAGTCA 
ACTATGTTAG AAGAATCTGT CTCCCCTAAA AAGTCCACCA TCCTGCAGCA GCAGTTCAAT 
CGCACCGGGA AGGTGGAACA TGGTTCGGTG GCCCTGCCAG CGGTAATGCG CTCTGGCTCC 
AGCGGGCCTG AGACCTTCAA TGTTGGCAGC ATGCCCTCCC CACAGCAGCA GGTCATGGTT 
GGGCAGATGC ACCGCGGTCA CATGCCCCCG CTGACCTCAG CCCAGCAGGC CCTCATGGGG 
ACCATCAACA CGAGCATGCA CGCTGTCCAG CAGGCCCAGG ATGACCTCAG CGAGCTCGAC 
TCCCTGCCAC CTCTCGGCCA GGATATGGCG TCCAGGGTGT GGGTTCAGAA TAAAGTGGAT 
GAATCCAAAC ACGAAATCCA CTCTCAAGTC GATGCCATCA CAGCTGGAAC CGCGTCAGTT 
GTTAACCTCA CAGCCGGTGA TCCTGCGGAC ACCGACTACA CTGCCGTGGG ATGCGCCATC 
ACCACCATTT CTTCCAACCT GACGGAGATG TCCAAGGGCG TGAAGCTGCT AGCAGCCCTC 
ATGGATGACG AGGTGGGCAG TGGGGAGGAC TTGCTCAGAG CCGCGAGGAC CCTCGCCGGG 
GCTGTGTCAG ATTTGCTGAA AGCTGTGCAG CCAACTTCTG GAGAGCCTCG ACAGACAGTT 
TTGACCAAAA AAGGAAGCAT AGGACAAGCC AGCGGGGATC TTCTGCGGCA GATCGGAGAG 
AATGAGACCG ACGAGCGATT CCAGGACGTT TTAATGAGTT TGGCTAAAGC TGTTGCCAAT 
GCTGCTGCCA TGTTGGTACT AAAGGCAAAG AATGTTGCCC AAGTGGCTGA GGACACGGTC 
CTGCAGAACA GGGTGATCGC CGCGGCCACC CAGTGTGCCC TCTCTACCTC CCAGCTAGTG 
GCGTGCGCCA AGGTCGTGAG CCCCACCATC AGCTCCCCCG TGTGCCAGGA GCAGCTGATC 
GAAGCAGGGA AGCTTGTGGA TCGCTCAGTA GAAAACTGTG TCCGTGCCTG CCAGGCGGCC 
ACCGACGACA CTGAGCTGCT GAAGCAGGTC AGCGCGGCTG CCAGCGTGGT CAGCCAGGCC 
CTCCACGACC TCCTGCAGCA CGTACGCCAG TTCGCCAGCC GAGGCGAGCC CATCGGTCGC 
TACGACCAGG CCACCGACAC CATCATGTGT GTCACCGAGA GCATCTTCAG CTCCATGGGC 
GATGCTGGTG AAATGGTACG CCAGGCCCGG GTCCTGGCCC AGGCCACCTC AGACCTCGTC 
AACGCCATGA GGTCAGACGC GGAAGCTGAA ATCGACATGG AGAATTCCAA GAAGCTCCTG 
GCAGCAGCTA AACTCTTGGC TGACTCCACA GCTCGCATGG TGGAGGCTGC AAAGGGGGCT 
GCAGCCAACC CTGAGAACGA GGACCAGCAG CAAAGGCTGA GAGAAGCTGC CGAAGGTCTC 
CGGGTAGCAA CCAATGCTGC AGCCCAGAAT GCTATTAAGA AAAAAATCGT CAACCGACTG 
GAGGTTGCAG CCAAGCAGGC TGCGGCTGCA GCCACACAGA CCATCGCCGC CTCCCAGAAC 
GCAGCCGTGT CCAACAAGAA CCCCGCGGCC CAGCAGCAGC TGGTGCAGAG CTGCAAGGCG 
GTGGCTGATC ACATTCCTCA GCTGGTCCAG GGGGTCAGAG GGAGCCAAGC TCAAGCAGAA 
GACCTGAGCG CCCAGCTGGC TCTCATCATC TCCAGCCAGA ACTTCCTCCA GCCTGGAAGC 
AAGATGGTAT CCTCTGCCAA AGCTGCAGTG CCCACGGTCA GCGACCAGGC TGCGGCCATG 
CAACTGAGCC AGTGTGCCAA GAACCTCGCC ACCAGCTTGG CAGAGCTGCG TACCGCCTCA 
CAGAAGGCCC ACGAAGCCTG TGGTCCCATG GAAATCGATT CAGCACTGAG CACCGTGCAG 
ACCCTGAAGA GTGAACTGCA GGACGCCAAG ATGGCGGCTG TGGAGAGTCA GCTGAAGCCA 
CTCCCCGGGG AGACGCTGGA AAAATGTGCT CAGGACCTGG GAAGTACATC TAAAGCAGTA 
GGCTCCTCCA TGGCGCAGCT CCTGACCTGT GCTGCTCAAG GCAATGAGCA CTACACAGGG 
GTGGCTGCTC GAGAGACGGC CCAAGCTCTG AAAACGCTGG CCCAGGCTGC CCGGGGCGTG 
GCTGCATCCA CGAGTGACCC GGCGGCTGCG CGCGCCATGT TGGATTCTGC CCGGGACGTG 
ATGGAGGGCT CGGCCATGCT CATCCAGGAG GCCAAACAGG CCCTGATTGC ACCCGGAGAC 
GCCGAGAGTC AGCAGAGACT AGCGCAGGTG GCCAAAGCCG TTTCTCACTC ATTGAATAAC 
TGTGTGAACT GTCTCCCTGG GCAGAAGGAT GTGGATGTGG CCTTGAAGAG CATTGGGGAG 
TCAAGCAAGA AGCTGCTGGT GGACTCGTTA CCGCCAAGCA CAAAGCCCTT CCAGGAAGCC 
CAGAGTGAAC TGAACCAAGC AGCCGCCGAT CTGAACCAGT CTGCGGGGGA TGTGGTCCAC 
GCCACCCGGG GCCAGAGTGG AGAGCTGGCC GCAGCCTCCG GGAAGTTCAG TGATGACTTC 
GATGAGTTCC TCGATGCTGG CATTGAGATG GCAGGCCAGG CGCAGACAAA AGAAGACCAG 
ATCCAACTCA TAGGGAACCT CAAGAATATC TCCATGGCCT CCAGCAAGCT ATTGTTAGCT 
GCCAAATCTC TCTCTGTAGA CCCAGGAGCC CCCAATGCGA AAAATCTCCT GGCTGCAGCT 
GCAAGAGCTG TGACTGAGAG CATCAACCAG CTCATCACGC TGTGCACGCA ACAAGCTCCT 
GGCCAGAAGG AATGTGATAA CGCCCTGCGG GAACTCGAGA CTGTCAAGGG GATGTTGGAC 
AATCCCAATG AACCTGTCAG TGACCTCTCG TACTTTGACT GCATCGAGAG TGTGATGGAA 
AACTCCAAGG TTCTGGGAGA ATCGATGGCA GGGATTTCGC AGAATGCCAA AACCGGGGAC 
CTCCCTGCCT TTGGGGAGTG TGTGGGGATT GCGTCCAAGG CTCTCTGTGG GCTGACGGAG 
GCTGCAGCCC AGGCTGCTTA CCTGGTCGGC GTCTCTGATC CAAACAGCCA GGCAGGCCAC 
CAGGGCCTTG TGGATCCCAT CCAGTTTGCC AGGGCCAACC AGGCTATCCA GATGGCGTGC 
CAGAACCTGG TGGATCCCGG CAGCAGCCCG TCACAGGTTT TGTCTGCTGC CACGATTGTC 
GCCAAGCACA CCTCAGCCCT GTGCAACGCC TGCCGAATCG CCTCATCCAA GACGGGCAAC 
CCCGTGGCCA AGAGGCACTT TGTCCAGTCC GCCAAGGAGG TCGCCAACAG CACCGCCAAC 
CTGGTGAAGA CCATAAAGGC CCTGGATGGG GACTTCTCCG AGGACAACCG CAACAAGTGT 
CGCGTCGCCA CTGCCCCCTT GATCGCAGCC GTGGAGAACT TGACGGCATT CGCCTCCAAC 
CCTGAGTTTG TCAGTGTTCC CGCCCAGATC AGCTCCGAGG GCTCCCAGGC ACAGGAACCA 
ATCCTGGTCT CAGCCAAGAC TATGCTGGAA AGCTCATCGT ACCTCATCCG CACTGCCCGC 
TCACTGGCCA TCAACCCCAA AGACCCGCCC ACCTGGTCTG TGCTAGCCGG ACATTCCCAC 
ACTGTGTCTG ACTCTATCAA GAGTCTCATC ACGTCCATCA GGGACAAGGC CCCGGGACAG 
AGAGAGTGTG ACTTCTCCAT CGACAGCATC AACCGGTGCA TCCGGGACAT CGAGCAGGCC 
TCGTTGGCGG CCGTCAGCCA GAGCTTGGCC ACGAGGGATG ACATCTCCGT GGAGGCCTTA 
CAGGAGCAGC TGACTTCAGT GGTCCAGGAA ATCGGGCACC TTATCGATCC CATTGCCACA 
GCGGCTCGGG GAGAAGCAGC TCAGCTGGGA CATAAGGTGA CACAGCTGGC GAGCTACTTT 
GAGCCCTTGA TCTTAGCTGC AGTGGGTGTC GCCTCCAAGA TTCTGGACCA TCAGCAGCAG 
ATGACGGTGC TGGACCAGAC CAAGACGCTC GCAGAGTCTG CCCTGCAGAT GCTGTATGCA 
GCCAAGGAAG GCGGAGGAAA CCCCAAGGCA CAGCACACCC ACGATGCCAT CACGGAGGCC 
GCCCAGCTCA TGAAAGAGGC CGTGGACGAC ATCATGGTGA CACTGAATGA AGCTGCCAGT 
GAAGTGGGGT TGGTGGGAGG TATGGTTGAC GCCATCGCAG AAGCCATGAG CAAGCTGGAT 
GAAAGTACTC CTCCAGAACC GAAGGGGACG TTTGTCGACT ATCAGACGAC GGTGGTTAAA 
TACTCCAAAG CTATCGCGGT AACAGCTCAG GAAATGATGA CTAAGTCGGT TACTAACCCG 
GAGGAGTTGG GAGGACTGGC TTCACAAATG ACCAGTGACT ATGGGCACCT GGCTCTCCAG 
GGCCAGCTGG CAGCAGCCAC GGCGGAACCA GAGGAGATCG GCTTCCAGAT TCGCACTCGC 
GTACAGGACC TGGGCCACGG CTGCATCTTC CTGGTGCAGA AGGCGGGGGC CCTCCAGGTG 
TGCCCCACGG ACAGCTACAC CAAGAGGGAG CTGATAGAGT GTGCCCGCGC TGTCACAGAG 
AAGGTATCCT TGGTGCTGTC CGCTCTCCAG GCTGGGAACA AGGGGACCCA GGCATGTATC 
ACAGCTGCCA CTGCCGTATC TGGGATCATT GCCGACCTGG ACACCACCAT CATGTTTGCG 
ACTGCAGGGA CCTTGAATGC AGAGAACAAC GAGACATTCG CAGACCACAG GGAGAACATT 
CTGAAGACAG CCAAGGCCTT GGTGGAAGAC ACAAAATTGC TGGTGTCGGG GGCTGCGTCC 
ACTCCAGACA AGCTGGCCCA GGCAGCTCAG TCCTCTGCAG CCACCATCAC CCAGCTCGCT 
GAGGTGGTCA AGCTGGGGGC CGCCAGCTTG GGCTCTGATG ACCCCGAGAC CCAGGTAGTG 
TTGATCAATG CCATCAAAGA CGTGGCCAAG GCCCTCTCAG ATCTCATTGG TGCTACCAAG 
GGAGCTGCCA GCAAGCCTGC CGATGACCCC TCCATGTACC AGCTCAAGGG GGCTGCCAAG 
GTGATGGTGA CCAATGTCAC CTCCCTCCTC AAGACCGTGA AGGCAGTGGA GGACGAGGCC 
ACTCGGGGCA CACGGGCACT AGAGGCCACC ATCGAGTACA TGAAGCAGGA GCTCACGGTA 
TTCCAGTCAA AAGAGGTACC TGAGAAGACA TCATCACCTG AAGAATCCAT CAGGATGACC 
AAAGGCATCA CCATGGCAAC GGCCAAAGCT GTGGCTGCTG GGAACTCTTG TCGACAGGAG 
GACGTGATAG CCACTGCCAA TCTGAGCCGG AAGGCCGTGT CAGACATGTT GACGGCCTGC 
AAGCAAGCTT CCTTCCACCC TGACGTCAGT GAGGAGGTGC GCACCAGAGC CTTGCGGTAT 
GGGACAGAGT GCACCCTCGG CTACCTGGAC CTCCTGGAGC ACGTCTTGGT GATTCTTCAG 
AAACCAACAC CAGAACTTAA GCACCAGCTG GCTGCTTTCT CCAAGCGCGT GGCTGGCGCC 
GTGACCGAGC TCATCCAGGC AGCAGAGGCC ATGAAAGGAA CAGAGTGGGT GGATCCCGAA 
GACCCAACCG TCATTGCAGA AACAGAATTA CTGGGGGCTG CAGCATCCAT TGAGGCTGCT 
GCTAAGAAGT TAGAGCAGCT GAAGCCAAGA GCAAAACCGA AACAAGCGGA CGAGACCCTG 
GATTTTGAAG AACAGATCTT GGAGGCTGCT AAATCCATTG CTGCTGCCAC GAGTGCCCTG 
GTCAAATCGG CCTCGGCAGC CCAGAGGGAG CTGGTGGCCC AAGGAAAGGT GGGTTCCATT 
CCTGCCAATG CTGCGGACGA CGGACAGTGG TCGCAGGGCC TGATTTCTGC CGCCCGGATG 
GTGGCAGCCG CGACCAGCAG TCTCTGCGAG GCAGCCAACG CCTCAGTGCA GGGACACGCC 
AGCGAGGAGA AGCTCATCTC ATCTGCCAAG CAGGTAGCTG CATCCACGGC TCAGCTGCTT 
GTGGCCTGCA AGGTGAAGGC TGATCAGGAT TCGGAGGCCA TGAGGCGGCT ACAGGCAGCA 
GGAAATGCTG TGAAAAGAGC CTCAGACAAC CTTGTTCGTG CAGCCCAGAA GGCAGCGTTT 
GGCAAAGCTG ATGATGACGA TGTTGTAGTG AAAACAAAGT TTGTAGGGGG CATTGCTCAG 
ATCATCGCCG CCCAGGAGGA AATGCTGAAG AAGGAACGAG AATTGGAAGA AGCAAGGAAA 
AAACTGGCCC AGATCCGCCA ACAGCAGTAT AAATTCCTAC CCACTGAGCT GAGAGAGGAC 
GAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TLN2
Entrez Gene ID	528252
Full Name	talin 2
General protein information	Preferred Names talin 2 Names talin-2 LOW QUALITY PROTEIN: talin-2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	TLN2	XP_510461.2
Bos taurus (cattle)	TLN2	XP_002690890.1
Rattus norvegicus (Norway rat)	Tln2	XP_006243460.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007474	XP_308398.3
Caenorhabditis elegans (roundworm)	Y71G12B.11	NP_490886.2
	TLN2	XP_001101705.1
Canis lupus familiaris (dog)	TLN2	XP_005638536.1
Drosophila melanogaster (fruit fly)	rhea	NP_001246674.1
Mus musculus (house mouse)	Tln2	NP_001074711.2
Gallus gallus (chicken)	TLN2	XP_003641925.1
Homo sapiens (human)	TLN2	NP_055874.2
Danio rerio (zebrafish)	tln2a	NP_957487.2
Xenopus tropicalis (tropical clawed frog)	tln2	NP_001116884.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TLN2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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