EFCAB5 cDNA ORF clone, Bos taurus(cattle)

The following EFCAB5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFCAB5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa10021 XM_024980696.1
Latest version!
Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa93792 XM_015467986.1
Latest version!
Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa93792 XM_015458590.2
Latest version!
Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa93793 XM_015458591.1
Latest version!
Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa93793 XM_015467987.1
Latest version!
Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa93794 XM_015458592.2
Latest version!
Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa93794 XM_015467988.1
Latest version!
Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
View Old Accession Versions >>
OBa93794 XM_015458592.1 Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa93792 XM_015458590.1 Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa10021
    Clone ID Related Accession (Same CDS sequence) XM_024980696.1
    Accession Version XM_024980696.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAGT CAGCACCTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAGGA ACCAGGAAGG AAGTGAAGCA GAAGAGCTTA TTCAGTCTTC ACAGAGTATA
    TCAGACAGTT CGGCATTAGG GACCCACAGC AATGTGATAG GAGCAGCAGC AAACAAAAAA
    TCCCAAGATT TAAACCTGGA GAACCAGCCA AAAATGTCAA CTTGTTCAAT AAAGGAGTCT
    AAAACTAGAG GCTCAAAGAA TATTGTCTTT AGAAAACCTG CAAAAGTGAT TTTCAGTTTA
    GATCAAACTG CACAGGATTC AAACCGGGAA CAACCATGGA AGAAAAATCT TTTAGAAAGG
    GTGCAGGCAA GAGCCCAGGT AATGCATCAG AAAATCATAG ATAAGGAAAA TCTGAAGAAG
    GAACTAGAAA AAAAGGAGGA AGAGAAACTC CCCAGGGATA ATTTGGCCAA AGAGTGGTTT
    AATACTGAAA ACATGACACT GAACACTCGT GCATATTTGC TCGACAAACT TCTACCCACC
    TTAGTTCCTG GAATGGAAAG AATGTTAATG CAAGTGGAAA AGAAGAAGCT TTTGGCAGAT
    GTTGATATTC CTACCAAGTT TGATCCAATT AACCATTTGG GAGAATACTT AATGAGAAAC
    AACCCTAATT ATATCAAAGA TTCAGGACTG TCTGGGTATC AGAGGGTGAT GAGAGAGATC
    TCAGAAGACC TGAAGATATA TGTTCCGAAC ACTACTTACA ACAGAGTATC CAAGATAAAG
    GAGAATATTA AACAGAAACG AGAACAAAAA GAATACATAA ACGACATCAA AGCCAAGGTG
    GCCAACGCAC GGAAACAGGC TCTGCAGGAG CAGTTCAATG AGTGGATCCT AGACCCCAAA
    GGAATGATTC CGATGGTAGT GATTCAGAAC GTTCTTTATG AATTTTTTCA AAATCCAGAT
    TTGCAGCTTG AAACATGCTG TCTACCAGTA GATATTGCTG ACTCAATGAA ACCAAGATTG
    AACAAAACAG AATTTATACA ACTCATCTCT TTGCACATTG CAGGCTTTAA GAGTGAAACG
    TTTGAGAAGC TCCTTAAGCA CCTTTGCCAT TGTGCTGCCG AATTCAGGGA GGTCATAAAA
    ACTGACATGC GGAGACAGAT GTTTGCTGAC CTCTTCCTGC ACTGTGACCG TGGGAAGGTA
    GGGTTTTTGG ATCGTCAGAG GACATTGGCC TTGCTGGAAA CATTCTATGA CCAAAGTTCA
    AAAGTGCTCA GAGGTACACT CCGAAACCCA AGACAATGGC CATTTGTTGA ATTTGGAGAG
    ATAGACTTAC CTGAGTTCTG GGGAGACATG GATAATCAGA AACACATTTA TGAAGACTTT
    GACAATGTTC TCTTAGAGAT GAATACGTTA CCTTCTGAAA AACATTTTAG CAAAACCCAA
    AGTAAATTAT TAGCAAGCCC AGAAGATCAA CATGAACACT ATAGAGAATC AACACTACCA
    CAAAGCCCAC CAGAACAGCA GAGAGGGGCA ACTGTGGAAC AAGGACCAAA TGGAATTTCA
    ACCAGAGAAC AAGAACAACC TGAAGAGTCA ACTGGAGAAC AAGAACTGAA CGAAGAATCA
    GTATCAAAAG AAGGAACCTA CACGGGGTCA ACTCCAGAAC AAAGATCAAG TAGAGAGTTA
    ATAACAGAAC AAAGACCACA TCATGAGCCA ATAACAGAAC AAGGAGTACC ACAAGGAACC
    CACATCGAAT CAACAGCAGA ACAAGGACTT TCTGAAGAAT CAATTACAAC AGAAGGACAA
    CATGAAGGGT CAACAACAGG ACAAGGATCA CACGGAAAGT CAACTGAGGA ACAGGGATCA
    CGCAGAGAGT CACAATCTGA TCAAGGACAA CCCAGAGAGA CAATGGCAGA GCAGGAAGCA
    CACAGAGAAT CACAGTCAGA TCAAGGACCA CACGGTGGGT CAACTGCAGA ACCACAGTCA
    TCCAGAGAAA CAATTCTGGA AGAACAACAG GACACAGACT TAACTTCACA ATCGAGAAAA
    GGTAGTCTCA CAGGAGAAAG CAAAACATCT AGGGAATCTA TTTCCTTTGA GCACACTGAA
    ATCCCTCCAC AGGAAGGAAG GACTCAAGCA CATGCATATG AAGAGCTACT CTTCGTAAGT
    CCTGAACTGC AAGCGAAAAC TGGAGTATCA ATCCCAGAAG ATCATCTTTC AGAACCTGCT
    AAAAAGGAAG TTCAGAAAGA CAAATCCTGC GAACCCAAGT CCCAGAAAAT AGAAGGAAAG
    TCATGGACAG GTGAACTTCT GACTTGTAAC TGGAACATGA AGTATGCCAA ACATGAAGAC
    GAGGAACAGG CACATTTAAT CTATGATAAC TCGAGGTTCA CAGACCTGCA CTCAATTATC
    AGAAACATTC AGTCTTACAA GGAGATAAAG GGTAGGAGTA CCTTCAACGG AGTTTCCGTC
    AATCTACTCC AGTTTGTGCA GCTCCTGGAG ACATTTGTTG GTGAAGATTC CCCCTTGAGT
    GTCAGTGAAG CCCTCACCTC CTTTTTTAGG AAGGGCTTTG TTGAAACAAA AGAAGAGAAA
    ATGAGTGGCT TAGAAAAGGC TAGACAAAAT GCTTCCCGAA TCCGACGGGA ACTTCTCCTA
    AAAGCCCTCT TTCAGAAGTG GGACTGTGAC GGCTCAGGCT TTCTGGATCT GAAGGAAATT
    GATGAACTCT TGTATACATA CAAGGAAGGA ATGGAAAAAG AATCTATGAA GAAAGCTAAA
    CTACATGTCC AATTTCCAAA GCTACACCCT GGGCACGAAG TGAGATTGTC TTCCAAACAG
    TTTCAGAAAT ACATAGAATT GGTTGTCTCT GAACTCAGGG GCAATGAAGA AGAAGTTCTG
    GAAAGTGTTG TGGAGTTCCT GATGAACTCT TTAGAAAGGA GCCATGTTGA GAGTCTGAGG
    AATTGTGCCA GACAAAAGTG GCTGCACCAA ATCCAGCGCG CTGCGGAGAC AAGTGGCGTG
    TCCTTGGAGC CAGTGTACAC GGAGACCTTT AAGGCCCTCA CACAGGATGC TGAAGCCCAT
    GGAAATAAGA AGATCAGTGC TCACATTTCC CTCTTAGAAG AAAACCTACT TCTGCCTGAT
    AGAGGGAACG TTCTATTGAG GAATGTAGCT TGTACTTTAG ATGATGCTCC ATTTGTTCTG
    AAAAGAGTGC TCTACAGGGA CATGAAGGGG ATCAGCTTTA CAGTAGTGGA TGAAGGGAAG
    CCAATACACG TCCCCCAAGT TCAGCACCAT GGGAACATCT ACTTCTGGAA CCATTCCCGC
    AAGAAGAGCG ATCACAATGG GTCATTCCTG GCTCTTCCCC TTCAGGACGC ATATATGAGG
    ATATTTGGGG TCTTGGCTGT TGACACTCTA AGAGATCCCC AGAAAATTAA CATCTTCCTA
    CCCCACGAGA TCAGATTCTA TCAGGGTGTT GCCAATGTCT TTAGTACTGC CTATCACTAC
    GTCCACAGCC GGGAGCACAT CCTTCATATT GTAATCACTG GCATCGGCTG GCTCTACAAC
    ATCACGCCCA GCATCACTGC CATCTCTACA TACTTTGTTG AGCCCAACTC AGAGAAGGAT
    TCAGACTATG TTTTACGAAA TATGATGGTC ACAGGGCAGC AGGGTCTAAC AGAAATCCAC
    AAGAATCCCT CTGTCATCTT TAGGAAGACA TGCATCTTCA GAGATTTCCT CTTTAAGTGC
    ACAGACAGTT CAGGAGTTGT TTTGGCCTCT GTCTGTGGAG AAAACCACAT CGTAGTTCCA
    CTTCGTGAGA GAACAGGAGA AGCTTTGGGA GTTCTCGATG TTAACATTGG CAAGAGTAAG
    ATGTTGTTGT ATCGAGAGTT TAAAGATCTA CAGAAAATGC TGAAGGTGAT CCAGCTTGCC
    TGCAGTGAAA TACTGGGCGA ATTCTCTGGA GAAATAAAGA AAAACTATAT CCTAGAGATT
    GAAAGTGTGG GAGAAGTCCA GCGGGCAGGC ATTCTCTTCT TCAGAGTCAT GCTACAGGAG
    CTACAGGAGT GCCTCAGTCT ACTTACATCC TCGGACTTTG TGTCACTGTT GCTGCATGAT
    TATGACCCTC TGGCGGAAGT CAAAGCCCCG CCAGACAGCC AGTCCCAGGA TCTGAAAGCC
    AACATAAAAT TAGTACAAGA CATCCTGAAA GGGATTATCG TGTTCTTTCA TCCAGAGTTG
    AAATCATCAA GTGACTTAGA AAGTTGGGAT CACTGTAAAC TGTACGTAAA CCAACAGTTA
    GTCGAAAATA TTTGTGACTT TGATCCAACT GCTATGGAAC TGAAAGTTAA TTTAAGGCTC
    ATTGCTGAAT ACTTTGAAAA TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATTCTGCC
    ATCGAATACT TATACCACTG GGCACACATC TGTTTAGCTC TCATAAAACT AAACAAAAAA
    ATAAGTGGTG CTATCCCCCC CCCACTGCCA TCCAAGACTG ACTCCTGTGT GTATGCAAAA
    ATGCCAGGGG GAAGCTTGCC AGGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836464.1
    CDS129..4658
    Translation

    Target ORF information:

    RefSeq Version XM_024980696.1
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980696.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAGT CAGCACCTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAGGA ACCAGGAAGG AAGTGAAGCA GAAGAGCTTA TTCAGTCTTC ACAGAGTATA
    TCAGACAGTT CGGCATTAGG GACCCACAGC AATGTGATAG GAGCAGCAGC AAACAAAAAA
    TCCCAAGATT TAAACCTGGA GAACCAGCCA AAAATGTCAA CTTGTTCAAT AAAGGAGTCT
    AAAACTAGAG GCTCAAAGAA TATTGTCTTT AGAAAACCTG CAAAAGTGAT TTTCAGTTTA
    GATCAAACTG CACAGGATTC AAACCGGGAA CAACCATGGA AGAAAAATCT TTTAGAAAGG
    GTGCAGGCAA GAGCCCAGGT AATGCATCAG AAAATCATAG ATAAGGAAAA TCTGAAGAAG
    GAACTAGAAA AAAAGGAGGA AGAGAAACTC CCCAGGGATA ATTTGGCCAA AGAGTGGTTT
    AATACTGAAA ACATGACACT GAACACTCGT GCATATTTGC TCGACAAACT TCTACCCACC
    TTAGTTCCTG GAATGGAAAG AATGTTAATG CAAGTGGAAA AGAAGAAGCT TTTGGCAGAT
    GTTGATATTC CTACCAAGTT TGATCCAATT AACCATTTGG GAGAATACTT AATGAGAAAC
    AACCCTAATT ATATCAAAGA TTCAGGACTG TCTGGGTATC AGAGGGTGAT GAGAGAGATC
    TCAGAAGACC TGAAGATATA TGTTCCGAAC ACTACTTACA ACAGAGTATC CAAGATAAAG
    GAGAATATTA AACAGAAACG AGAACAAAAA GAATACATAA ACGACATCAA AGCCAAGGTG
    GCCAACGCAC GGAAACAGGC TCTGCAGGAG CAGTTCAATG AGTGGATCCT AGACCCCAAA
    GGAATGATTC CGATGGTAGT GATTCAGAAC GTTCTTTATG AATTTTTTCA AAATCCAGAT
    TTGCAGCTTG AAACATGCTG TCTACCAGTA GATATTGCTG ACTCAATGAA ACCAAGATTG
    AACAAAACAG AATTTATACA ACTCATCTCT TTGCACATTG CAGGCTTTAA GAGTGAAACG
    TTTGAGAAGC TCCTTAAGCA CCTTTGCCAT TGTGCTGCCG AATTCAGGGA GGTCATAAAA
    ACTGACATGC GGAGACAGAT GTTTGCTGAC CTCTTCCTGC ACTGTGACCG TGGGAAGGTA
    GGGTTTTTGG ATCGTCAGAG GACATTGGCC TTGCTGGAAA CATTCTATGA CCAAAGTTCA
    AAAGTGCTCA GAGGTACACT CCGAAACCCA AGACAATGGC CATTTGTTGA ATTTGGAGAG
    ATAGACTTAC CTGAGTTCTG GGGAGACATG GATAATCAGA AACACATTTA TGAAGACTTT
    GACAATGTTC TCTTAGAGAT GAATACGTTA CCTTCTGAAA AACATTTTAG CAAAACCCAA
    AGTAAATTAT TAGCAAGCCC AGAAGATCAA CATGAACACT ATAGAGAATC AACACTACCA
    CAAAGCCCAC CAGAACAGCA GAGAGGGGCA ACTGTGGAAC AAGGACCAAA TGGAATTTCA
    ACCAGAGAAC AAGAACAACC TGAAGAGTCA ACTGGAGAAC AAGAACTGAA CGAAGAATCA
    GTATCAAAAG AAGGAACCTA CACGGGGTCA ACTCCAGAAC AAAGATCAAG TAGAGAGTTA
    ATAACAGAAC AAAGACCACA TCATGAGCCA ATAACAGAAC AAGGAGTACC ACAAGGAACC
    CACATCGAAT CAACAGCAGA ACAAGGACTT TCTGAAGAAT CAATTACAAC AGAAGGACAA
    CATGAAGGGT CAACAACAGG ACAAGGATCA CACGGAAAGT CAACTGAGGA ACAGGGATCA
    CGCAGAGAGT CACAATCTGA TCAAGGACAA CCCAGAGAGA CAATGGCAGA GCAGGAAGCA
    CACAGAGAAT CACAGTCAGA TCAAGGACCA CACGGTGGGT CAACTGCAGA ACCACAGTCA
    TCCAGAGAAA CAATTCTGGA AGAACAACAG GACACAGACT TAACTTCACA ATCGAGAAAA
    GGTAGTCTCA CAGGAGAAAG CAAAACATCT AGGGAATCTA TTTCCTTTGA GCACACTGAA
    ATCCCTCCAC AGGAAGGAAG GACTCAAGCA CATGCATATG AAGAGCTACT CTTCGTAAGT
    CCTGAACTGC AAGCGAAAAC TGGAGTATCA ATCCCAGAAG ATCATCTTTC AGAACCTGCT
    AAAAAGGAAG TTCAGAAAGA CAAATCCTGC GAACCCAAGT CCCAGAAAAT AGAAGGAAAG
    TCATGGACAG GTGAACTTCT GACTTGTAAC TGGAACATGA AGTATGCCAA ACATGAAGAC
    GAGGAACAGG CACATTTAAT CTATGATAAC TCGAGGTTCA CAGACCTGCA CTCAATTATC
    AGAAACATTC AGTCTTACAA GGAGATAAAG GGTAGGAGTA CCTTCAACGG AGTTTCCGTC
    AATCTACTCC AGTTTGTGCA GCTCCTGGAG ACATTTGTTG GTGAAGATTC CCCCTTGAGT
    GTCAGTGAAG CCCTCACCTC CTTTTTTAGG AAGGGCTTTG TTGAAACAAA AGAAGAGAAA
    ATGAGTGGCT TAGAAAAGGC TAGACAAAAT GCTTCCCGAA TCCGACGGGA ACTTCTCCTA
    AAAGCCCTCT TTCAGAAGTG GGACTGTGAC GGCTCAGGCT TTCTGGATCT GAAGGAAATT
    GATGAACTCT TGTATACATA CAAGGAAGGA ATGGAAAAAG AATCTATGAA GAAAGCTAAA
    CTACATGTCC AATTTCCAAA GCTACACCCT GGGCACGAAG TGAGATTGTC TTCCAAACAG
    TTTCAGAAAT ACATAGAATT GGTTGTCTCT GAACTCAGGG GCAATGAAGA AGAAGTTCTG
    GAAAGTGTTG TGGAGTTCCT GATGAACTCT TTAGAAAGGA GCCATGTTGA GAGTCTGAGG
    AATTGTGCCA GACAAAAGTG GCTGCACCAA ATCCAGCGCG CTGCGGAGAC AAGTGGCGTG
    TCCTTGGAGC CAGTGTACAC GGAGACCTTT AAGGCCCTCA CACAGGATGC TGAAGCCCAT
    GGAAATAAGA AGATCAGTGC TCACATTTCC CTCTTAGAAG AAAACCTACT TCTGCCTGAT
    AGAGGGAACG TTCTATTGAG GAATGTAGCT TGTACTTTAG ATGATGCTCC ATTTGTTCTG
    AAAAGAGTGC TCTACAGGGA CATGAAGGGG ATCAGCTTTA CAGTAGTGGA TGAAGGGAAG
    CCAATACACG TCCCCCAAGT TCAGCACCAT GGGAACATCT ACTTCTGGAA CCATTCCCGC
    AAGAAGAGCG ATCACAATGG GTCATTCCTG GCTCTTCCCC TTCAGGACGC ATATATGAGG
    ATATTTGGGG TCTTGGCTGT TGACACTCTA AGAGATCCCC AGAAAATTAA CATCTTCCTA
    CCCCACGAGA TCAGATTCTA TCAGGGTGTT GCCAATGTCT TTAGTACTGC CTATCACTAC
    GTCCACAGCC GGGAGCACAT CCTTCATATT GTAATCACTG GCATCGGCTG GCTCTACAAC
    ATCACGCCCA GCATCACTGC CATCTCTACA TACTTTGTTG AGCCCAACTC AGAGAAGGAT
    TCAGACTATG TTTTACGAAA TATGATGGTC ACAGGGCAGC AGGGTCTAAC AGAAATCCAC
    AAGAATCCCT CTGTCATCTT TAGGAAGACA TGCATCTTCA GAGATTTCCT CTTTAAGTGC
    ACAGACAGTT CAGGAGTTGT TTTGGCCTCT GTCTGTGGAG AAAACCACAT CGTAGTTCCA
    CTTCGTGAGA GAACAGGAGA AGCTTTGGGA GTTCTCGATG TTAACATTGG CAAGAGTAAG
    ATGTTGTTGT ATCGAGAGTT TAAAGATCTA CAGAAAATGC TGAAGGTGAT CCAGCTTGCC
    TGCAGTGAAA TACTGGGCGA ATTCTCTGGA GAAATAAAGA AAAACTATAT CCTAGAGATT
    GAAAGTGTGG GAGAAGTCCA GCGGGCAGGC ATTCTCTTCT TCAGAGTCAT GCTACAGGAG
    CTACAGGAGT GCCTCAGTCT ACTTACATCC TCGGACTTTG TGTCACTGTT GCTGCATGAT
    TATGACCCTC TGGCGGAAGT CAAAGCCCCG CCAGACAGCC AGTCCCAGGA TCTGAAAGCC
    AACATAAAAT TAGTACAAGA CATCCTGAAA GGGATTATCG TGTTCTTTCA TCCAGAGTTG
    AAATCATCAA GTGACTTAGA AAGTTGGGAT CACTGTAAAC TGTACGTAAA CCAACAGTTA
    GTCGAAAATA TTTGTGACTT TGATCCAACT GCTATGGAAC TGAAAGTTAA TTTAAGGCTC
    ATTGCTGAAT ACTTTGAAAA TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATTCTGCC
    ATCGAATACT TATACCACTG GGCACACATC TGTTTAGCTC TCATAAAACT AAACAAAAAA
    ATAAGTGGTG CTATCCCCCC CCCACTGCCA TCCAAGACTG ACTCCTGTGT GTATGCAAAA
    ATGCCAGGGG GAAGCTTGCC AGGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93792
    Clone ID Related Accession (Same CDS sequence) XM_015458590.1 , XM_015467986.1 , XM_015458590.2
    Accession Version XM_015458590.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT AAACTACATG TCCAATTTCC AAAGCTACAC
    CCTGGGCACG AAGTGAGATT GTCTTCCAAA CAGTTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGAAGAAGTT CTGGAAAGTG TTGTGGAGTT CCTGATGAAC
    TCTTTAGAAA GGAGCCATGT TGAGAGTCTG AGGAATTGTG CCAGACAAAA GTGGCTGCAC
    CAAATCCAGC GCGCTGCGGA GACAAGTGGC GTGTCCTTGG AGCCAGTGTA CACGGAGACC
    TTTAAGGCCC TCACACAGGA TGCTGAAGCC CATGGAAATA AGAAGATCAG TGCTCACATT
    TCCCTCTTAG AAGAAAACCT ACTTCTGCCT GATAGAGGGA ACGTTCTATT GAGGAATGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTT CTGAAAAGAG TGCTCTACAG GGACATGAAG
    GGGATCAGCT TTACAGTAGT GGATGAAGGG AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACA TCTACTTCTG GAACCATTCC CGCAAGAAGA GCGATCACAA TGGGTCATTC
    CTGGCTCTTC CCCTTCAGGA CGCATATATG AGGATATTTG GGGTCTTGGC TGTTGACACT
    CTAAGAGATC CCCAGAAAAT TAACATCTTC CTACCCCACG AGATCAGATT CTATCAGGGT
    GTTGCCAATG TCTTTAGTAC TGCCTATCAC TACGTCCACA GCCGGGAGCA CATCCTTCAT
    ATTGTAATCA CTGGCATCGG CTGGCTCTAC AACATCACGC CCAGCATCAC TGCCATCTCT
    ACATACTTTG TTGAGCCCAA CTCAGAGAAG GATTCAGACT ATGTTTTACG AAATATGATG
    GTCACAGGGC AGCAGGGTCT AACAGAAATC CACAAGAATC CCTCTGTCAT CTTTAGGAAG
    ACATGCATCT TCAGAGATTT CCTCTTTAAG TGCACAGACA GTTCAGGAGT TGTTTTGGCC
    TCTGTCTGTG GAGAAAACCA CATCGTAGTT CCACTTCGTG AGAGAACAGG AGAAGCTTTG
    GGAGTTCTCG ATGTTAACAT TGGCAAGAGT AAGATGTTGT TGTATCGAGA GTTTAAAGAT
    CTACAGAAAA TGCTGAAGGT GATCCAGCTT GCCTGCAGTG AAATACTGGG CGAATTCTCT
    GGAGAAATAA AGAAAAACTA TATCCTAGAG ATTGAAAGTG TGGGAGAAGT CCAGCGGGCA
    GGCATTCTCT TCTTCAGAGT CATGCTACAG GAGCTACAGG AGTGCCTCAG TCTACTTACA
    TCCTCGGACT TTGTGTCACT GTTGCTGCAT GATTATGACC CTCTGGCGGA AGTCAAAGCC
    CCGCCAGACA GCCAGTCCCA GGATCTGAAA GCCAACATAA AATTAGTACA AGACATCCTG
    AAAGGGATTA TCGTGTTCTT TCATCCAGAG TTGAAATCAT CAAGTGACTT AGAAAGTTGG
    GATCACTGTA AACTGTACGT AAACCAACAG TTAGTCGAAA ATATTTGTGA CTTTGATCCA
    ACTGCTATGG AACTGAAAGT TAATTTAAGG CTCATTGCTG AATACTTTGA AAATCATTCT
    CGAATTGAAG TATGGAAATT TGGTAATTCT GCCATCGAAT ACTTATACCA CTGGGCACAC
    ATCTGTTTAG CTCTCATAAA ACTAAACAAA AAAATAAGTG GTGCTATCCC CCCCCCACTG
    CCATCCAAGA CTGACTCCTG TGTGTATGCA AAAATGCCAG GGGGAAGCTT GCCAGGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314076.1
    CDS61..4623
    Translation

    Target ORF information:

    RefSeq Version XM_015458590.1
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458590.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT AAACTACATG TCCAATTTCC AAAGCTACAC
    CCTGGGCACG AAGTGAGATT GTCTTCCAAA CAGTTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGAAGAAGTT CTGGAAAGTG TTGTGGAGTT CCTGATGAAC
    TCTTTAGAAA GGAGCCATGT TGAGAGTCTG AGGAATTGTG CCAGACAAAA GTGGCTGCAC
    CAAATCCAGC GCGCTGCGGA GACAAGTGGC GTGTCCTTGG AGCCAGTGTA CACGGAGACC
    TTTAAGGCCC TCACACAGGA TGCTGAAGCC CATGGAAATA AGAAGATCAG TGCTCACATT
    TCCCTCTTAG AAGAAAACCT ACTTCTGCCT GATAGAGGGA ACGTTCTATT GAGGAATGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTT CTGAAAAGAG TGCTCTACAG GGACATGAAG
    GGGATCAGCT TTACAGTAGT GGATGAAGGG AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACA TCTACTTCTG GAACCATTCC CGCAAGAAGA GCGATCACAA TGGGTCATTC
    CTGGCTCTTC CCCTTCAGGA CGCATATATG AGGATATTTG GGGTCTTGGC TGTTGACACT
    CTAAGAGATC CCCAGAAAAT TAACATCTTC CTACCCCACG AGATCAGATT CTATCAGGGT
    GTTGCCAATG TCTTTAGTAC TGCCTATCAC TACGTCCACA GCCGGGAGCA CATCCTTCAT
    ATTGTAATCA CTGGCATCGG CTGGCTCTAC AACATCACGC CCAGCATCAC TGCCATCTCT
    ACATACTTTG TTGAGCCCAA CTCAGAGAAG GATTCAGACT ATGTTTTACG AAATATGATG
    GTCACAGGGC AGCAGGGTCT AACAGAAATC CACAAGAATC CCTCTGTCAT CTTTAGGAAG
    ACATGCATCT TCAGAGATTT CCTCTTTAAG TGCACAGACA GTTCAGGAGT TGTTTTGGCC
    TCTGTCTGTG GAGAAAACCA CATCGTAGTT CCACTTCGTG AGAGAACAGG AGAAGCTTTG
    GGAGTTCTCG ATGTTAACAT TGGCAAGAGT AAGATGTTGT TGTATCGAGA GTTTAAAGAT
    CTACAGAAAA TGCTGAAGGT GATCCAGCTT GCCTGCAGTG AAATACTGGG CGAATTCTCT
    GGAGAAATAA AGAAAAACTA TATCCTAGAG ATTGAAAGTG TGGGAGAAGT CCAGCGGGCA
    GGCATTCTCT TCTTCAGAGT CATGCTACAG GAGCTACAGG AGTGCCTCAG TCTACTTACA
    TCCTCGGACT TTGTGTCACT GTTGCTGCAT GATTATGACC CTCTGGCGGA AGTCAAAGCC
    CCGCCAGACA GCCAGTCCCA GGATCTGAAA GCCAACATAA AATTAGTACA AGACATCCTG
    AAAGGGATTA TCGTGTTCTT TCATCCAGAG TTGAAATCAT CAAGTGACTT AGAAAGTTGG
    GATCACTGTA AACTGTACGT AAACCAACAG TTAGTCGAAA ATATTTGTGA CTTTGATCCA
    ACTGCTATGG AACTGAAAGT TAATTTAAGG CTCATTGCTG AATACTTTGA AAATCATTCT
    CGAATTGAAG TATGGAAATT TGGTAATTCT GCCATCGAAT ACTTATACCA CTGGGCACAC
    ATCTGTTTAG CTCTCATAAA ACTAAACAAA AAAATAAGTG GTGCTATCCC CCCCCCACTG
    CCATCCAAGA CTGACTCCTG TGTGTATGCA AAAATGCCAG GGGGAAGCTT GCCAGGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93792
    Clone ID Related Accession (Same CDS sequence) XM_015458590.1 , XM_015467986.1 , XM_015458590.2
    Accession Version XM_015467986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT AAACTACATG TCCAATTTCC AAAGCTACAC
    CCTGGGCACG AAGTGAGATT GTCTTCCAAA CAGTTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGAAGAAGTT CTGGAAAGTG TTGTGGAGTT CCTGATGAAC
    TCTTTAGAAA GGAGCCATGT TGAGAGTCTG AGGAATTGTG CCAGACAAAA GTGGCTGCAC
    CAAATCCAGC GCGCTGCGGA GACAAGTGGC GTGTCCTTGG AGCCAGTGTA CACGGAGACC
    TTTAAGGCCC TCACACAGGA TGCTGAAGCC CATGGAAATA AGAAGATCAG TGCTCACATT
    TCCCTCTTAG AAGAAAACCT ACTTCTGCCT GATAGAGGGA ACGTTCTATT GAGGAATGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTT CTGAAAAGAG TGCTCTACAG GGACATGAAG
    GGGATCAGCT TTACAGTAGT GGATGAAGGG AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACA TCTACTTCTG GAACCATTCC CGCAAGAAGA GCGATCACAA TGGGTCATTC
    CTGGCTCTTC CCCTTCAGGA CGCATATATG AGGATATTTG GGGTCTTGGC TGTTGACACT
    CTAAGAGATC CCCAGAAAAT TAACATCTTC CTACCCCACG AGATCAGATT CTATCAGGGT
    GTTGCCAATG TCTTTAGTAC TGCCTATCAC TACGTCCACA GCCGGGAGCA CATCCTTCAT
    ATTGTAATCA CTGGCATCGG CTGGCTCTAC AACATCACGC CCAGCATCAC TGCCATCTCT
    ACATACTTTG TTGAGCCCAA CTCAGAGAAG GATTCAGACT ATGTTTTACG AAATATGATG
    GTCACAGGGC AGCAGGGTCT AACAGAAATC CACAAGAATC CCTCTGTCAT CTTTAGGAAG
    ACATGCATCT TCAGAGATTT CCTCTTTAAG TGCACAGACA GTTCAGGAGT TGTTTTGGCC
    TCTGTCTGTG GAGAAAACCA CATCGTAGTT CCACTTCGTG AGAGAACAGG AGAAGCTTTG
    GGAGTTCTCG ATGTTAACAT TGGCAAGAGT AAGATGTTGT TGTATCGAGA GTTTAAAGAT
    CTACAGAAAA TGCTGAAGGT GATCCAGCTT GCCTGCAGTG AAATACTGGG CGAATTCTCT
    GGAGAAATAA AGAAAAACTA TATCCTAGAG ATTGAAAGTG TGGGAGAAGT CCAGCGGGCA
    GGCATTCTCT TCTTCAGAGT CATGCTACAG GAGCTACAGG AGTGCCTCAG TCTACTTACA
    TCCTCGGACT TTGTGTCACT GTTGCTGCAT GATTATGACC CTCTGGCGGA AGTCAAAGCC
    CCGCCAGACA GCCAGTCCCA GGATCTGAAA GCCAACATAA AATTAGTACA AGACATCCTG
    AAAGGGATTA TCGTGTTCTT TCATCCAGAG TTGAAATCAT CAAGTGACTT AGAAAGTTGG
    GATCACTGTA AACTGTACGT AAACCAACAG TTAGTCGAAA ATATTTGTGA CTTTGATCCA
    ACTGCTATGG AACTGAAAGT TAATTTAAGG CTCATTGCTG AATACTTTGA AAATCATTCT
    CGAATTGAAG TATGGAAATT TGGTAATTCT GCCATCGAAT ACTTATACCA CTGGGCACAC
    ATCTGTTTAG CTCTCATAAA ACTAAACAAA AAAATAAGTG GTGCTATCCC CCCCCCACTG
    CCATCCAAGA CTGACTCCTG TGTGTATGCA AAAATGCCAG GGGGAAGCTT GCCAGGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323472.1
    CDS61..4623
    Translation

    Target ORF information:

    RefSeq Version XM_015467986.1
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT AAACTACATG TCCAATTTCC AAAGCTACAC
    CCTGGGCACG AAGTGAGATT GTCTTCCAAA CAGTTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGAAGAAGTT CTGGAAAGTG TTGTGGAGTT CCTGATGAAC
    TCTTTAGAAA GGAGCCATGT TGAGAGTCTG AGGAATTGTG CCAGACAAAA GTGGCTGCAC
    CAAATCCAGC GCGCTGCGGA GACAAGTGGC GTGTCCTTGG AGCCAGTGTA CACGGAGACC
    TTTAAGGCCC TCACACAGGA TGCTGAAGCC CATGGAAATA AGAAGATCAG TGCTCACATT
    TCCCTCTTAG AAGAAAACCT ACTTCTGCCT GATAGAGGGA ACGTTCTATT GAGGAATGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTT CTGAAAAGAG TGCTCTACAG GGACATGAAG
    GGGATCAGCT TTACAGTAGT GGATGAAGGG AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACA TCTACTTCTG GAACCATTCC CGCAAGAAGA GCGATCACAA TGGGTCATTC
    CTGGCTCTTC CCCTTCAGGA CGCATATATG AGGATATTTG GGGTCTTGGC TGTTGACACT
    CTAAGAGATC CCCAGAAAAT TAACATCTTC CTACCCCACG AGATCAGATT CTATCAGGGT
    GTTGCCAATG TCTTTAGTAC TGCCTATCAC TACGTCCACA GCCGGGAGCA CATCCTTCAT
    ATTGTAATCA CTGGCATCGG CTGGCTCTAC AACATCACGC CCAGCATCAC TGCCATCTCT
    ACATACTTTG TTGAGCCCAA CTCAGAGAAG GATTCAGACT ATGTTTTACG AAATATGATG
    GTCACAGGGC AGCAGGGTCT AACAGAAATC CACAAGAATC CCTCTGTCAT CTTTAGGAAG
    ACATGCATCT TCAGAGATTT CCTCTTTAAG TGCACAGACA GTTCAGGAGT TGTTTTGGCC
    TCTGTCTGTG GAGAAAACCA CATCGTAGTT CCACTTCGTG AGAGAACAGG AGAAGCTTTG
    GGAGTTCTCG ATGTTAACAT TGGCAAGAGT AAGATGTTGT TGTATCGAGA GTTTAAAGAT
    CTACAGAAAA TGCTGAAGGT GATCCAGCTT GCCTGCAGTG AAATACTGGG CGAATTCTCT
    GGAGAAATAA AGAAAAACTA TATCCTAGAG ATTGAAAGTG TGGGAGAAGT CCAGCGGGCA
    GGCATTCTCT TCTTCAGAGT CATGCTACAG GAGCTACAGG AGTGCCTCAG TCTACTTACA
    TCCTCGGACT TTGTGTCACT GTTGCTGCAT GATTATGACC CTCTGGCGGA AGTCAAAGCC
    CCGCCAGACA GCCAGTCCCA GGATCTGAAA GCCAACATAA AATTAGTACA AGACATCCTG
    AAAGGGATTA TCGTGTTCTT TCATCCAGAG TTGAAATCAT CAAGTGACTT AGAAAGTTGG
    GATCACTGTA AACTGTACGT AAACCAACAG TTAGTCGAAA ATATTTGTGA CTTTGATCCA
    ACTGCTATGG AACTGAAAGT TAATTTAAGG CTCATTGCTG AATACTTTGA AAATCATTCT
    CGAATTGAAG TATGGAAATT TGGTAATTCT GCCATCGAAT ACTTATACCA CTGGGCACAC
    ATCTGTTTAG CTCTCATAAA ACTAAACAAA AAAATAAGTG GTGCTATCCC CCCCCCACTG
    CCATCCAAGA CTGACTCCTG TGTGTATGCA AAAATGCCAG GGGGAAGCTT GCCAGGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93792
    Clone ID Related Accession (Same CDS sequence) XM_015458590.1 , XM_015467986.1 , XM_015458590.2
    Accession Version XM_015458590.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT AAACTACATG TCCAATTTCC AAAGCTACAC
    CCTGGGCACG AAGTGAGATT GTCTTCCAAA CAGTTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGAAGAAGTT CTGGAAAGTG TTGTGGAGTT CCTGATGAAC
    TCTTTAGAAA GGAGCCATGT TGAGAGTCTG AGGAATTGTG CCAGACAAAA GTGGCTGCAC
    CAAATCCAGC GCGCTGCGGA GACAAGTGGC GTGTCCTTGG AGCCAGTGTA CACGGAGACC
    TTTAAGGCCC TCACACAGGA TGCTGAAGCC CATGGAAATA AGAAGATCAG TGCTCACATT
    TCCCTCTTAG AAGAAAACCT ACTTCTGCCT GATAGAGGGA ACGTTCTATT GAGGAATGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTT CTGAAAAGAG TGCTCTACAG GGACATGAAG
    GGGATCAGCT TTACAGTAGT GGATGAAGGG AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACA TCTACTTCTG GAACCATTCC CGCAAGAAGA GCGATCACAA TGGGTCATTC
    CTGGCTCTTC CCCTTCAGGA CGCATATATG AGGATATTTG GGGTCTTGGC TGTTGACACT
    CTAAGAGATC CCCAGAAAAT TAACATCTTC CTACCCCACG AGATCAGATT CTATCAGGGT
    GTTGCCAATG TCTTTAGTAC TGCCTATCAC TACGTCCACA GCCGGGAGCA CATCCTTCAT
    ATTGTAATCA CTGGCATCGG CTGGCTCTAC AACATCACGC CCAGCATCAC TGCCATCTCT
    ACATACTTTG TTGAGCCCAA CTCAGAGAAG GATTCAGACT ATGTTTTACG AAATATGATG
    GTCACAGGGC AGCAGGGTCT AACAGAAATC CACAAGAATC CCTCTGTCAT CTTTAGGAAG
    ACATGCATCT TCAGAGATTT CCTCTTTAAG TGCACAGACA GTTCAGGAGT TGTTTTGGCC
    TCTGTCTGTG GAGAAAACCA CATCGTAGTT CCACTTCGTG AGAGAACAGG AGAAGCTTTG
    GGAGTTCTCG ATGTTAACAT TGGCAAGAGT AAGATGTTGT TGTATCGAGA GTTTAAAGAT
    CTACAGAAAA TGCTGAAGGT GATCCAGCTT GCCTGCAGTG AAATACTGGG CGAATTCTCT
    GGAGAAATAA AGAAAAACTA TATCCTAGAG ATTGAAAGTG TGGGAGAAGT CCAGCGGGCA
    GGCATTCTCT TCTTCAGAGT CATGCTACAG GAGCTACAGG AGTGCCTCAG TCTACTTACA
    TCCTCGGACT TTGTGTCACT GTTGCTGCAT GATTATGACC CTCTGGCGGA AGTCAAAGCC
    CCGCCAGACA GCCAGTCCCA GGATCTGAAA GCCAACATAA AATTAGTACA AGACATCCTG
    AAAGGGATTA TCGTGTTCTT TCATCCAGAG TTGAAATCAT CAAGTGACTT AGAAAGTTGG
    GATCACTGTA AACTGTACGT AAACCAACAG TTAGTCGAAA ATATTTGTGA CTTTGATCCA
    ACTGCTATGG AACTGAAAGT TAATTTAAGG CTCATTGCTG AATACTTTGA AAATCATTCT
    CGAATTGAAG TATGGAAATT TGGTAATTCT GCCATCGAAT ACTTATACCA CTGGGCACAC
    ATCTGTTTAG CTCTCATAAA ACTAAACAAA AAAATAAGTG GTGCTATCCC CCCCCCACTG
    CCATCCAAGA CTGACTCCTG TGTGTATGCA AAAATGCCAG GGGGAAGCTT GCCAGGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314076.1
    CDS60..4622
    Translation

    Target ORF information:

    RefSeq Version XM_015458590.2
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458590.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT AAACTACATG TCCAATTTCC AAAGCTACAC
    CCTGGGCACG AAGTGAGATT GTCTTCCAAA CAGTTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGAAGAAGTT CTGGAAAGTG TTGTGGAGTT CCTGATGAAC
    TCTTTAGAAA GGAGCCATGT TGAGAGTCTG AGGAATTGTG CCAGACAAAA GTGGCTGCAC
    CAAATCCAGC GCGCTGCGGA GACAAGTGGC GTGTCCTTGG AGCCAGTGTA CACGGAGACC
    TTTAAGGCCC TCACACAGGA TGCTGAAGCC CATGGAAATA AGAAGATCAG TGCTCACATT
    TCCCTCTTAG AAGAAAACCT ACTTCTGCCT GATAGAGGGA ACGTTCTATT GAGGAATGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTT CTGAAAAGAG TGCTCTACAG GGACATGAAG
    GGGATCAGCT TTACAGTAGT GGATGAAGGG AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACA TCTACTTCTG GAACCATTCC CGCAAGAAGA GCGATCACAA TGGGTCATTC
    CTGGCTCTTC CCCTTCAGGA CGCATATATG AGGATATTTG GGGTCTTGGC TGTTGACACT
    CTAAGAGATC CCCAGAAAAT TAACATCTTC CTACCCCACG AGATCAGATT CTATCAGGGT
    GTTGCCAATG TCTTTAGTAC TGCCTATCAC TACGTCCACA GCCGGGAGCA CATCCTTCAT
    ATTGTAATCA CTGGCATCGG CTGGCTCTAC AACATCACGC CCAGCATCAC TGCCATCTCT
    ACATACTTTG TTGAGCCCAA CTCAGAGAAG GATTCAGACT ATGTTTTACG AAATATGATG
    GTCACAGGGC AGCAGGGTCT AACAGAAATC CACAAGAATC CCTCTGTCAT CTTTAGGAAG
    ACATGCATCT TCAGAGATTT CCTCTTTAAG TGCACAGACA GTTCAGGAGT TGTTTTGGCC
    TCTGTCTGTG GAGAAAACCA CATCGTAGTT CCACTTCGTG AGAGAACAGG AGAAGCTTTG
    GGAGTTCTCG ATGTTAACAT TGGCAAGAGT AAGATGTTGT TGTATCGAGA GTTTAAAGAT
    CTACAGAAAA TGCTGAAGGT GATCCAGCTT GCCTGCAGTG AAATACTGGG CGAATTCTCT
    GGAGAAATAA AGAAAAACTA TATCCTAGAG ATTGAAAGTG TGGGAGAAGT CCAGCGGGCA
    GGCATTCTCT TCTTCAGAGT CATGCTACAG GAGCTACAGG AGTGCCTCAG TCTACTTACA
    TCCTCGGACT TTGTGTCACT GTTGCTGCAT GATTATGACC CTCTGGCGGA AGTCAAAGCC
    CCGCCAGACA GCCAGTCCCA GGATCTGAAA GCCAACATAA AATTAGTACA AGACATCCTG
    AAAGGGATTA TCGTGTTCTT TCATCCAGAG TTGAAATCAT CAAGTGACTT AGAAAGTTGG
    GATCACTGTA AACTGTACGT AAACCAACAG TTAGTCGAAA ATATTTGTGA CTTTGATCCA
    ACTGCTATGG AACTGAAAGT TAATTTAAGG CTCATTGCTG AATACTTTGA AAATCATTCT
    CGAATTGAAG TATGGAAATT TGGTAATTCT GCCATCGAAT ACTTATACCA CTGGGCACAC
    ATCTGTTTAG CTCTCATAAA ACTAAACAAA AAAATAAGTG GTGCTATCCC CCCCCCACTG
    CCATCCAAGA CTGACTCCTG TGTGTATGCA AAAATGCCAG GGGGAAGCTT GCCAGGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93793
    Clone ID Related Accession (Same CDS sequence) XM_015458591.1 , XM_015467987.1
    Accession Version XM_015458591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGACCTGCA CTCAATTATC
    AGAAACATTC AGTCTTACAA GGAGATAAAG GGTAGGAGTA CCTTCAACGG AGTTTCCGTC
    AATCTACTCC AGTTTGTGCA GCTCCTGGAG ACATTTGTTG GTGAAGATTC CCCCTTGAGT
    GTCAGTGAAG CCCTCACCTC CTTTTTTAGG AAGGGCTTTG TTGAAACAAA AGAAGAGAAA
    ATGAGTGGCT TAGAAAAGGC TAGACAAAAT GCTTCCCGAA TCCGACGGGA ACTTCTCCTA
    AAAGCCCTCT TTCAGAAGTG GGACTGTGAC GGCTCAGGCT TTCTGGATCT GAAGGAAATT
    GATGAACTCT TGTATACATA CAAGGAAGGA ATGGAAAAAG AATCTATGAA GAAAGCTAAA
    CTACATGTCC AATTTCCAAA GCTACACCCT GGGCACGAAG TGAGATTGTC TTCCAAACAG
    TTTCAGAAAT ACATAGAATT GGTTGTCTCT GAACTCAGGG GCAATGAAGA AGAAGTTCTG
    GAAAGTGTTG TGGAGTTCCT GATGAACTCT TTAGAAAGGA GCCATGTTGA GAGTCTGAGG
    AATTGTGCCA GACAAAAGTG GCTGCACCAA ATCCAGCGCG CTGCGGAGAC AAGTGGCGTG
    TCCTTGGAGC CAGTGTACAC GGAGACCTTT AAGGCCCTCA CACAGGATGC TGAAGCCCAT
    GGAAATAAGA AGATCAGTGC TCACATTTCC CTCTTAGAAG AAAACCTACT TCTGCCTGAT
    AGAGGGAACG TTCTATTGAG GAATGTAGCT TGTACTTTAG ATGATGCTCC ATTTGTTCTG
    AAAAGAGTGC TCTACAGGGA CATGAAGGGG ATCAGCTTTA CAGTAGTGGA TGAAGGGAAG
    CCAATACACG TCCCCCAAGT TCAGCACCAT GGGAACATCT ACTTCTGGAA CCATTCCCGC
    AAGAAGAGCG ATCACAATGG GTCATTCCTG GCTCTTCCCC TTCAGGACGC ATATATGAGG
    ATATTTGGGG TCTTGGCTGT TGACACTCTA AGAGATCCCC AGAAAATTAA CATCTTCCTA
    CCCCACGAGA TCAGATTCTA TCAGGGTGTT GCCAATGTCT TTAGTACTGC CTATCACTAC
    GTCCACAGCC GGGAGCACAT CCTTCATATT GTAATCACTG GCATCGGCTG GCTCTACAAC
    ATCACGCCCA GCATCACTGC CATCTCTACA TACTTTGTTG AGCCCAACTC AGAGAAGGAT
    TCAGACTATG TTTTACGAAA TATGATGGTC ACAGGGCAGC AGGGTCTAAC AGAAATCCAC
    AAGAATCCCT CTGTCATCTT TAGGAAGACA TGCATCTTCA GAGATTTCCT CTTTAAGTGC
    ACAGACAGTT CAGGAGTTGT TTTGGCCTCT GTCTGTGGAG AAAACCACAT CGTAGTTCCA
    CTTCGTGAGA GAACAGGAGA AGCTTTGGGA GTTCTCGATG TTAACATTGG CAAGAGTAAG
    ATGTTGTTGT ATCGAGAGTT TAAAGATCTA CAGAAAATGC TGAAGGTGAT CCAGCTTGCC
    TGCAGTGAAA TACTGGGCGA ATTCTCTGGA GAAATAAAGA AAAACTATAT CCTAGAGATT
    GAAAGTGTGG GAGAAGTCCA GCGGGCAGGC ATTCTCTTCT TCAGAGTCAT GCTACAGGAG
    CTACAGGAGT GCCTCAGTCT ACTTACATCC TCGGACTTTG TGTCACTGTT GCTGCATGAT
    TATGACCCTC TGGCGGAAGT CAAAGCCCCG CCAGACAGCC AGTCCCAGGA TCTGAAAGCC
    AACATAAAAT TAGTACAAGA CATCCTGAAA GGGATTATCG TGTTCTTTCA TCCAGAGTTG
    AAATCATCAA GTGACTTAGA AAGTTGGGAT CACTGTAAAC TGTACGTAAA CCAACAGTTA
    GTCGAAAATA TTTGTGACTT TGATCCAACT GCTATGGAAC TGAAAGTTAA TTTAAGGCTC
    ATTGCTGAAT ACTTTGAAAA TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATTCTGCC
    ATCGAATACT TATACCACTG GGCACACATC TGTTTAGCTC TCATAAAACT AAACAAAAAA
    ATAAGTGGTG CTATCCCCCC CCCACTGCCA TCCAAGACTG ACTCCTGTGT GTATGCAAAA
    ATGCCAGGGG GAAGCTTGCC AGGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314077.1
    CDS61..4530
    Translation

    Target ORF information:

    RefSeq Version XM_015458591.1
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGACCTGCA CTCAATTATC
    AGAAACATTC AGTCTTACAA GGAGATAAAG GGTAGGAGTA CCTTCAACGG AGTTTCCGTC
    AATCTACTCC AGTTTGTGCA GCTCCTGGAG ACATTTGTTG GTGAAGATTC CCCCTTGAGT
    GTCAGTGAAG CCCTCACCTC CTTTTTTAGG AAGGGCTTTG TTGAAACAAA AGAAGAGAAA
    ATGAGTGGCT TAGAAAAGGC TAGACAAAAT GCTTCCCGAA TCCGACGGGA ACTTCTCCTA
    AAAGCCCTCT TTCAGAAGTG GGACTGTGAC GGCTCAGGCT TTCTGGATCT GAAGGAAATT
    GATGAACTCT TGTATACATA CAAGGAAGGA ATGGAAAAAG AATCTATGAA GAAAGCTAAA
    CTACATGTCC AATTTCCAAA GCTACACCCT GGGCACGAAG TGAGATTGTC TTCCAAACAG
    TTTCAGAAAT ACATAGAATT GGTTGTCTCT GAACTCAGGG GCAATGAAGA AGAAGTTCTG
    GAAAGTGTTG TGGAGTTCCT GATGAACTCT TTAGAAAGGA GCCATGTTGA GAGTCTGAGG
    AATTGTGCCA GACAAAAGTG GCTGCACCAA ATCCAGCGCG CTGCGGAGAC AAGTGGCGTG
    TCCTTGGAGC CAGTGTACAC GGAGACCTTT AAGGCCCTCA CACAGGATGC TGAAGCCCAT
    GGAAATAAGA AGATCAGTGC TCACATTTCC CTCTTAGAAG AAAACCTACT TCTGCCTGAT
    AGAGGGAACG TTCTATTGAG GAATGTAGCT TGTACTTTAG ATGATGCTCC ATTTGTTCTG
    AAAAGAGTGC TCTACAGGGA CATGAAGGGG ATCAGCTTTA CAGTAGTGGA TGAAGGGAAG
    CCAATACACG TCCCCCAAGT TCAGCACCAT GGGAACATCT ACTTCTGGAA CCATTCCCGC
    AAGAAGAGCG ATCACAATGG GTCATTCCTG GCTCTTCCCC TTCAGGACGC ATATATGAGG
    ATATTTGGGG TCTTGGCTGT TGACACTCTA AGAGATCCCC AGAAAATTAA CATCTTCCTA
    CCCCACGAGA TCAGATTCTA TCAGGGTGTT GCCAATGTCT TTAGTACTGC CTATCACTAC
    GTCCACAGCC GGGAGCACAT CCTTCATATT GTAATCACTG GCATCGGCTG GCTCTACAAC
    ATCACGCCCA GCATCACTGC CATCTCTACA TACTTTGTTG AGCCCAACTC AGAGAAGGAT
    TCAGACTATG TTTTACGAAA TATGATGGTC ACAGGGCAGC AGGGTCTAAC AGAAATCCAC
    AAGAATCCCT CTGTCATCTT TAGGAAGACA TGCATCTTCA GAGATTTCCT CTTTAAGTGC
    ACAGACAGTT CAGGAGTTGT TTTGGCCTCT GTCTGTGGAG AAAACCACAT CGTAGTTCCA
    CTTCGTGAGA GAACAGGAGA AGCTTTGGGA GTTCTCGATG TTAACATTGG CAAGAGTAAG
    ATGTTGTTGT ATCGAGAGTT TAAAGATCTA CAGAAAATGC TGAAGGTGAT CCAGCTTGCC
    TGCAGTGAAA TACTGGGCGA ATTCTCTGGA GAAATAAAGA AAAACTATAT CCTAGAGATT
    GAAAGTGTGG GAGAAGTCCA GCGGGCAGGC ATTCTCTTCT TCAGAGTCAT GCTACAGGAG
    CTACAGGAGT GCCTCAGTCT ACTTACATCC TCGGACTTTG TGTCACTGTT GCTGCATGAT
    TATGACCCTC TGGCGGAAGT CAAAGCCCCG CCAGACAGCC AGTCCCAGGA TCTGAAAGCC
    AACATAAAAT TAGTACAAGA CATCCTGAAA GGGATTATCG TGTTCTTTCA TCCAGAGTTG
    AAATCATCAA GTGACTTAGA AAGTTGGGAT CACTGTAAAC TGTACGTAAA CCAACAGTTA
    GTCGAAAATA TTTGTGACTT TGATCCAACT GCTATGGAAC TGAAAGTTAA TTTAAGGCTC
    ATTGCTGAAT ACTTTGAAAA TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATTCTGCC
    ATCGAATACT TATACCACTG GGCACACATC TGTTTAGCTC TCATAAAACT AAACAAAAAA
    ATAAGTGGTG CTATCCCCCC CCCACTGCCA TCCAAGACTG ACTCCTGTGT GTATGCAAAA
    ATGCCAGGGG GAAGCTTGCC AGGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93793
    Clone ID Related Accession (Same CDS sequence) XM_015458591.1 , XM_015467987.1
    Accession Version XM_015467987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGACCTGCA CTCAATTATC
    AGAAACATTC AGTCTTACAA GGAGATAAAG GGTAGGAGTA CCTTCAACGG AGTTTCCGTC
    AATCTACTCC AGTTTGTGCA GCTCCTGGAG ACATTTGTTG GTGAAGATTC CCCCTTGAGT
    GTCAGTGAAG CCCTCACCTC CTTTTTTAGG AAGGGCTTTG TTGAAACAAA AGAAGAGAAA
    ATGAGTGGCT TAGAAAAGGC TAGACAAAAT GCTTCCCGAA TCCGACGGGA ACTTCTCCTA
    AAAGCCCTCT TTCAGAAGTG GGACTGTGAC GGCTCAGGCT TTCTGGATCT GAAGGAAATT
    GATGAACTCT TGTATACATA CAAGGAAGGA ATGGAAAAAG AATCTATGAA GAAAGCTAAA
    CTACATGTCC AATTTCCAAA GCTACACCCT GGGCACGAAG TGAGATTGTC TTCCAAACAG
    TTTCAGAAAT ACATAGAATT GGTTGTCTCT GAACTCAGGG GCAATGAAGA AGAAGTTCTG
    GAAAGTGTTG TGGAGTTCCT GATGAACTCT TTAGAAAGGA GCCATGTTGA GAGTCTGAGG
    AATTGTGCCA GACAAAAGTG GCTGCACCAA ATCCAGCGCG CTGCGGAGAC AAGTGGCGTG
    TCCTTGGAGC CAGTGTACAC GGAGACCTTT AAGGCCCTCA CACAGGATGC TGAAGCCCAT
    GGAAATAAGA AGATCAGTGC TCACATTTCC CTCTTAGAAG AAAACCTACT TCTGCCTGAT
    AGAGGGAACG TTCTATTGAG GAATGTAGCT TGTACTTTAG ATGATGCTCC ATTTGTTCTG
    AAAAGAGTGC TCTACAGGGA CATGAAGGGG ATCAGCTTTA CAGTAGTGGA TGAAGGGAAG
    CCAATACACG TCCCCCAAGT TCAGCACCAT GGGAACATCT ACTTCTGGAA CCATTCCCGC
    AAGAAGAGCG ATCACAATGG GTCATTCCTG GCTCTTCCCC TTCAGGACGC ATATATGAGG
    ATATTTGGGG TCTTGGCTGT TGACACTCTA AGAGATCCCC AGAAAATTAA CATCTTCCTA
    CCCCACGAGA TCAGATTCTA TCAGGGTGTT GCCAATGTCT TTAGTACTGC CTATCACTAC
    GTCCACAGCC GGGAGCACAT CCTTCATATT GTAATCACTG GCATCGGCTG GCTCTACAAC
    ATCACGCCCA GCATCACTGC CATCTCTACA TACTTTGTTG AGCCCAACTC AGAGAAGGAT
    TCAGACTATG TTTTACGAAA TATGATGGTC ACAGGGCAGC AGGGTCTAAC AGAAATCCAC
    AAGAATCCCT CTGTCATCTT TAGGAAGACA TGCATCTTCA GAGATTTCCT CTTTAAGTGC
    ACAGACAGTT CAGGAGTTGT TTTGGCCTCT GTCTGTGGAG AAAACCACAT CGTAGTTCCA
    CTTCGTGAGA GAACAGGAGA AGCTTTGGGA GTTCTCGATG TTAACATTGG CAAGAGTAAG
    ATGTTGTTGT ATCGAGAGTT TAAAGATCTA CAGAAAATGC TGAAGGTGAT CCAGCTTGCC
    TGCAGTGAAA TACTGGGCGA ATTCTCTGGA GAAATAAAGA AAAACTATAT CCTAGAGATT
    GAAAGTGTGG GAGAAGTCCA GCGGGCAGGC ATTCTCTTCT TCAGAGTCAT GCTACAGGAG
    CTACAGGAGT GCCTCAGTCT ACTTACATCC TCGGACTTTG TGTCACTGTT GCTGCATGAT
    TATGACCCTC TGGCGGAAGT CAAAGCCCCG CCAGACAGCC AGTCCCAGGA TCTGAAAGCC
    AACATAAAAT TAGTACAAGA CATCCTGAAA GGGATTATCG TGTTCTTTCA TCCAGAGTTG
    AAATCATCAA GTGACTTAGA AAGTTGGGAT CACTGTAAAC TGTACGTAAA CCAACAGTTA
    GTCGAAAATA TTTGTGACTT TGATCCAACT GCTATGGAAC TGAAAGTTAA TTTAAGGCTC
    ATTGCTGAAT ACTTTGAAAA TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATTCTGCC
    ATCGAATACT TATACCACTG GGCACACATC TGTTTAGCTC TCATAAAACT AAACAAAAAA
    ATAAGTGGTG CTATCCCCCC CCCACTGCCA TCCAAGACTG ACTCCTGTGT GTATGCAAAA
    ATGCCAGGGG GAAGCTTGCC AGGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323473.1
    CDS61..4530
    Translation

    Target ORF information:

    RefSeq Version XM_015467987.1
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGACCTGCA CTCAATTATC
    AGAAACATTC AGTCTTACAA GGAGATAAAG GGTAGGAGTA CCTTCAACGG AGTTTCCGTC
    AATCTACTCC AGTTTGTGCA GCTCCTGGAG ACATTTGTTG GTGAAGATTC CCCCTTGAGT
    GTCAGTGAAG CCCTCACCTC CTTTTTTAGG AAGGGCTTTG TTGAAACAAA AGAAGAGAAA
    ATGAGTGGCT TAGAAAAGGC TAGACAAAAT GCTTCCCGAA TCCGACGGGA ACTTCTCCTA
    AAAGCCCTCT TTCAGAAGTG GGACTGTGAC GGCTCAGGCT TTCTGGATCT GAAGGAAATT
    GATGAACTCT TGTATACATA CAAGGAAGGA ATGGAAAAAG AATCTATGAA GAAAGCTAAA
    CTACATGTCC AATTTCCAAA GCTACACCCT GGGCACGAAG TGAGATTGTC TTCCAAACAG
    TTTCAGAAAT ACATAGAATT GGTTGTCTCT GAACTCAGGG GCAATGAAGA AGAAGTTCTG
    GAAAGTGTTG TGGAGTTCCT GATGAACTCT TTAGAAAGGA GCCATGTTGA GAGTCTGAGG
    AATTGTGCCA GACAAAAGTG GCTGCACCAA ATCCAGCGCG CTGCGGAGAC AAGTGGCGTG
    TCCTTGGAGC CAGTGTACAC GGAGACCTTT AAGGCCCTCA CACAGGATGC TGAAGCCCAT
    GGAAATAAGA AGATCAGTGC TCACATTTCC CTCTTAGAAG AAAACCTACT TCTGCCTGAT
    AGAGGGAACG TTCTATTGAG GAATGTAGCT TGTACTTTAG ATGATGCTCC ATTTGTTCTG
    AAAAGAGTGC TCTACAGGGA CATGAAGGGG ATCAGCTTTA CAGTAGTGGA TGAAGGGAAG
    CCAATACACG TCCCCCAAGT TCAGCACCAT GGGAACATCT ACTTCTGGAA CCATTCCCGC
    AAGAAGAGCG ATCACAATGG GTCATTCCTG GCTCTTCCCC TTCAGGACGC ATATATGAGG
    ATATTTGGGG TCTTGGCTGT TGACACTCTA AGAGATCCCC AGAAAATTAA CATCTTCCTA
    CCCCACGAGA TCAGATTCTA TCAGGGTGTT GCCAATGTCT TTAGTACTGC CTATCACTAC
    GTCCACAGCC GGGAGCACAT CCTTCATATT GTAATCACTG GCATCGGCTG GCTCTACAAC
    ATCACGCCCA GCATCACTGC CATCTCTACA TACTTTGTTG AGCCCAACTC AGAGAAGGAT
    TCAGACTATG TTTTACGAAA TATGATGGTC ACAGGGCAGC AGGGTCTAAC AGAAATCCAC
    AAGAATCCCT CTGTCATCTT TAGGAAGACA TGCATCTTCA GAGATTTCCT CTTTAAGTGC
    ACAGACAGTT CAGGAGTTGT TTTGGCCTCT GTCTGTGGAG AAAACCACAT CGTAGTTCCA
    CTTCGTGAGA GAACAGGAGA AGCTTTGGGA GTTCTCGATG TTAACATTGG CAAGAGTAAG
    ATGTTGTTGT ATCGAGAGTT TAAAGATCTA CAGAAAATGC TGAAGGTGAT CCAGCTTGCC
    TGCAGTGAAA TACTGGGCGA ATTCTCTGGA GAAATAAAGA AAAACTATAT CCTAGAGATT
    GAAAGTGTGG GAGAAGTCCA GCGGGCAGGC ATTCTCTTCT TCAGAGTCAT GCTACAGGAG
    CTACAGGAGT GCCTCAGTCT ACTTACATCC TCGGACTTTG TGTCACTGTT GCTGCATGAT
    TATGACCCTC TGGCGGAAGT CAAAGCCCCG CCAGACAGCC AGTCCCAGGA TCTGAAAGCC
    AACATAAAAT TAGTACAAGA CATCCTGAAA GGGATTATCG TGTTCTTTCA TCCAGAGTTG
    AAATCATCAA GTGACTTAGA AAGTTGGGAT CACTGTAAAC TGTACGTAAA CCAACAGTTA
    GTCGAAAATA TTTGTGACTT TGATCCAACT GCTATGGAAC TGAAAGTTAA TTTAAGGCTC
    ATTGCTGAAT ACTTTGAAAA TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATTCTGCC
    ATCGAATACT TATACCACTG GGCACACATC TGTTTAGCTC TCATAAAACT AAACAAAAAA
    ATAAGTGGTG CTATCCCCCC CCCACTGCCA TCCAAGACTG ACTCCTGTGT GTATGCAAAA
    ATGCCAGGGG GAAGCTTGCC AGGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93794
    Clone ID Related Accession (Same CDS sequence) XM_015458592.2 , XM_015467988.1 , XM_015458592.1
    Accession Version XM_015458592.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT TTACAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314078.1
    CDS61..2859
    Translation

    Target ORF information:

    RefSeq Version XM_015458592.2
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458592.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT TTACAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93794
    Clone ID Related Accession (Same CDS sequence) XM_015458592.2 , XM_015467988.1 , XM_015458592.1
    Accession Version XM_015467988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT TTACAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323474.1
    CDS61..2859
    Translation

    Target ORF information:

    RefSeq Version XM_015467988.1
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT TTACAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93794
    Clone ID Related Accession (Same CDS sequence) XM_015458592.2 , XM_015467988.1 , XM_015458592.1
    Accession Version XM_015458592.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT TTACAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314078.1
    CDS61..2859
    Translation

    Target ORF information:

    RefSeq Version XM_015458592.1
    Organism Bos taurus(cattle)
    Definition Bos taurus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCTAGAGT ATAAAACATT AGTTTTATTT CCAGCCCCTA TTGAATACCA TTTACTAAAA 
    CTATGTCCAT TTCAGGAAAA TGGAAAAGAA GACAAAGAAA GGAACCAGGA AGGAAGTGAA
    GCAGAAGAGC TTATTCAGTC TTCACAGAGT ATATCAGACA GTTCGGCATT AGGGACCCAC
    AGCAATGTGA TAGGAGCAGC AGCAAACAAA AAATCCCAAG ATTTAAACCT GGAGAACCAG
    CCAAAAATGT CAACTTGTTC AATAAAGGAG TCTAAAACTA GAGGCTCAAA GAATATTGTC
    TTTAGAAAAC CTGCAAAAGT GATTTTCAGT TTAGATCAAA CTGCACAGGA TTCAAACCGG
    GAACAACCAT GGAAGAAAAA TCTTTTAGAA AGGGTGCAGG CAAGAGCCCA GGTAATGCAT
    CAGAAAATCA TAGATAAGGA AAATCTGAAG AAGGAACTAG AAAAAAAGGA GGAAGAGAAA
    CTCCCCAGGG ATAATTTGGC CAAAGAGTGG TTTAATACTG AAAACATGAC ACTGAACACT
    CGTGCATATT TGCTCGACAA ACTTCTACCC ACCTTAGTTC CTGGAATGGA AAGAATGTTA
    ATGCAAGTGG AAAAGAAGAA GCTTTTGGCA GATGTTGATA TTCCTACCAA GTTTGATCCA
    ATTAACCATT TGGGAGAATA CTTAATGAGA AACAACCCTA ATTATATCAA AGATTCAGGA
    CTGTCTGGGT ATCAGAGGGT GATGAGAGAG ATCTCAGAAG ACCTGAAGAT ATATGTTCCG
    AACACTACTT ACAACAGAGT ATCCAAGATA AAGGAGAATA TTAAACAGAA ACGAGAACAA
    AAAGAATACA TAAACGACAT CAAAGCCAAG GTGGCCAACG CACGGAAACA GGCTCTGCAG
    GAGCAGTTCA ATGAGTGGAT CCTAGACCCC AAAGGAATGA TTCCGATGGT AGTGATTCAG
    AACGTTCTTT ATGAATTTTT TCAAAATCCA GATTTGCAGC TTGAAACATG CTGTCTACCA
    GTAGATATTG CTGACTCAAT GAAACCAAGA TTGAACAAAA CAGAATTTAT ACAACTCATC
    TCTTTGCACA TTGCAGGCTT TAAGAGTGAA ACGTTTGAGA AGCTCCTTAA GCACCTTTGC
    CATTGTGCTG CCGAATTCAG GGAGGTCATA AAAACTGACA TGCGGAGACA GATGTTTGCT
    GACCTCTTCC TGCACTGTGA CCGTGGGAAG GTAGGGTTTT TGGATCGTCA GAGGACATTG
    GCCTTGCTGG AAACATTCTA TGACCAAAGT TCAAAAGTGC TCAGAGGTAC ACTCCGAAAC
    CCAAGACAAT GGCCATTTGT TGAATTTGGA GAGATAGACT TACCTGAGTT CTGGGGAGAC
    ATGGATAATC AGAAACACAT TTATGAAGAC TTTGACAATG TTCTCTTAGA GATGAATACG
    TTACCTTCTG AAAAACATTT TAGCAAAACC CAAAGTAAAT TATTAGCAAG CCCAGAAGAT
    CAACATGAAC ACTATAGAGA ATCAACACTA CCACAAAGCC CACCAGAACA GCAGAGAGGG
    GCAACTGTGG AACAAGGACC AAATGGAATT TCAACCAGAG AACAAGAACA ACCTGAAGAG
    TCAACTGGAG AACAAGAACT GAACGAAGAA TCAGTATCAA AAGAAGGAAC CTACACGGGG
    TCAACTCCAG AACAAAGATC AAGTAGAGAG TTAATAACAG AACAAAGACC ACATCATGAG
    CCAATAACAG AACAAGGAGT ACCACAAGGA ACCCACATCG AATCAACAGC AGAACAAGGA
    CTTTCTGAAG AATCAATTAC AACAGAAGGA CAACATGAAG GGTCAACAAC AGGACAAGGA
    TCACACGGAA AGTCAACTGA GGAACAGGGA TCACGCAGAG AGTCACAATC TGATCAAGGA
    CAACCCAGAG AGACAATGGC AGAGCAGGAA GCACACAGAG AATCACAGTC AGATCAAGGA
    CCACACGGTG GGTCAACTGC AGAACCACAG TCATCCAGAG AAACAATTCT GGAAGAACAA
    CAGGACACAG ACTTAACTTC ACAATCGAGA AAAGGTAGTC TCACAGGAGA AAGCAAAACA
    TCTAGGGAAT CTATTTCCTT TGAGCACACT GAAATCCCTC CACAGGAAGG AAGGACTCAA
    GCACATGCAT ATGAAGAGCT ACTCTTCGTA AGTCCTGAAC TGCAAGCGAA AACTGGAGTA
    TCAATCCCAG AAGATCATCT TTCAGAACCT GCTAAAAAGG AAGTTCAGAA AGACAAATCC
    TGCGAACCCA AGTCCCAGAA AATAGAAGGA AAGTCATGGA CAGGTGAACT TCTGACTTGT
    AACTGGAACA TGAAGTATGC CAAACATGAA GACGAGGAAC AGGCACATTT AATCTATGAT
    AACTCGAGGT TCACAGACCT GCACTCAATT ATCAGAAACA TTCAGTCTTA CAAGGAGATA
    AAGGGTAGGA GTACCTTCAA CGGAGTTTCC GTCAATCTAC TCCAGTTTGT GCAGCTCCTG
    GAGACATTTG TTGGTGAAGA TTCCCCCTTG AGTGTCAGTG AAGCCCTCAC CTCCTTTTTT
    AGGAAGGGCT TTGTTGAAAC AAAAGAAGAG AAAATGAGTG GCTTAGAAAA GGCTAGACAA
    AATGCTTCCC GAATCCGACG GGAACTTCTC CTAAAAGCCC TCTTTCAGAA GTGGGACTGT
    GACGGCTCAG GCTTTCTGGA TCTGAAGGAA ATTGATGAAC TCTTGTATAC ATACAAGGAA
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT TTACAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.