EFCAB5 cDNA ORF clone, Homo sapiens(human)

The following EFCAB5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFCAB5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu02607 NM_198529.3
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu108319 NM_001145053.2
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu104399 XM_024450742.1
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu104400 XM_024450743.1
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu108333 NM_198529.4
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu02362 NM_001145053.1
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu79645 XM_011524759.1
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu79647 XM_011524763.2
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu79649 XM_011524765.1
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu79650 XM_011524766.2
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu79652 XM_011524768.2
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu86835 XM_017024591.1
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu86836 XM_017024592.1
Latest version!
Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu02607
    Clone ID Related Accession (Same CDS sequence) NM_198529.3
    Accession Version NM_198529.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-20
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB064946.1, DB080627.1, BC137519.1, BC110609.1 and AL833911.1. On Feb 10, 2009 this sequence version replaced NM_198529.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC137519.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CAAAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGATTCAG AATGTTCTTC AAGAATTCTT TCAAAATCCA
    GATTTCAAGC TTGGATCACA CTGCAAACAA CTGGATATTA CTGACTCAAC AGAACCAAGA
    TTGAACAAAA TGGAATTTAC AGAATACATC TCTTCACATA TTAAAGACTT GAAGAGTGAA
    ATGTTTGAGG AACTTCTTAA GCACCTTTGC CACTCTGCAG ATGAATTTCG GGAGGTCATA
    AAAGCTGACA TGCGGAGGCA GATGTTCGCT GAACTCTTCC TACATTGTGA CCACGGGAAG
    GTAGGGTTTT TGGATCGGCA GAGGACATTG GCCCTGCTGG AATTGTTCTA TGACCATAGT
    TCACAAATGC TTAGGAGTTT ACTTCGAAAC CCACGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAAACT TGACTGAGTT GTGGGGAGAC ATGGATAATC AGAAACACAT TTATGAAGGT
    TTTGACAAAG TGCTCTTGGA GATGAATACA TTACTTTCTG CAAATCATGC TAGCAAAACC
    CAAAGTAAAT TATTAGAAAG TCCAGATCAA CCTAAACTTA ACGAACAGAG AACATCAACA
    CCATCACCAA ACCCGCCAGA ACAGCAGAGA GGAGTAACTG CAGAACAAGG ACCACAAAGA
    ATTTCAATTG AAGAACAACA ACAAGGCAAA AAGCCAACTG CAGAGCAAGA ACTGTACATA
    GAATCAGTAA TAGAACCAGG AACACACACA GAGTCAACTC TAGAACAAGG GTCAAGTAGA
    AGGTTACTGA CAGAACAAGA AACACACAGA GAGTCAACTA CAGAACAAGG ACAGCACAAA
    GGGTCAATAG AAGGACAAGG ACCACGCAGA GTGTCAGTTT CAGAACAAGG ATCAAGCAGA
    GAGTCAGTTG CAGAACAAGG GTCACGCAGA GAGTCTATTG CAGAACAAGA TCGACACAAA
    GGGTCAGTAG CAGAACAAGG ATCACGCAGA ATGTCAGCTG CAGAACAGGG ATCACTCAGA
    GAGTCAGTAA TAGAAGAACC ATACCAAAAA TCAGAACAAG GACCTTATGG AGAGATAATT
    TCAGAAGAGC AAGAAGACAT AGGCTCAACT TCACAATCAA GAAAAGATAG TATCTTAAAA
    AGTACAAAAT ATGGGGAACC TATAACCTCT GAGTACATTG AAGTCCCTCT ACAGGAAAAG
    AGGTCTTGGG AACAAACATA TGAAGAGGAA ATATTCCTGA GTTCTGAACT GCAAGAGGAA
    GTTCCAACCT TAAGCAGAAA AGATCACTTT CCAGAAACTA CAAAAAAGGA AGTTCAGAAA
    GACAAGCCCT GTGAACCCAA GTCCCAAAAA ATAGAAGGAA AGTCATGGTC AGGTGAATTT
    TTTACTTGTA ACTGGAAAAT GAAGTATGTC ACATTTGAAG ATGAGGAACA GGCAAACTTA
    ATCTATGGTA ACTCCAGGTT CACAGATTTA CACTCAATTA TCAGAAATAT TCAGTCTTGC
    AAGGAGGTAA AAGGCAGAAC TGCCTTCAAT GGAGTTTCAT TCAATCTGCT CCAGTTTGTG
    CAACTCCTGG AGACATTTGT TGGTGAAGAC GCTCCCTTGA GTGTCAGCGA GACTCTCACC
    TCCTTTTTTA AGGAGGGCTA TGTTGAAACA GAACAAGAGA AAATGAATGC CTTAGAACAG
    TTTAGCCAAA ATGCTTTCCA AGTCCGACAG AGGCTTCTCC TAGAAGCCAT CTTTCAGAAG
    TGGGACAGTG ATGGCTCAGG CTTCCTGGAT CTGAAGGAAG TTGATGAACT CTTGTACACA
    TACAAGGAGG GAATGGAAAA AGAATCTATG AAGAAAGCTA AACTACACAT CCAATTTCCA
    AAGCCACACC CTGGTCACGA AGTGAGATTG TCTTCAAAAC AATTTCAGAA TTACATAGAA
    TTGGTTGTGT CTGAACTCAG GGGCAATGAA GACCAAGTTC TGGAAAGTGT TGTGGAATTT
    CTGATGAATG CTTTAGAGAG GAGCCACATT GAGAGTCTGA GGAATTCTGC CAGGCGGAAA
    TGGCTGCACC AAATCCAATG TGCTGCAGAG ACAAGTGGGG TGTCCCTAGA GCCGGTGTAT
    AGTGAGACCT TTAAGGCCCT CATGCAGGAT GCTGAAGCCC ATGGAAATAA AAAGATCAGT
    GCTCACATTT CCCTCTTAGA AGAAAACCTA CTACTGCCTG AGAAAGGGAA TGTTCTATTG
    AGGAATGTGG CTTGTACCTT AGATGATGCT CAATTTGTAC TGAACAGAGT GCTCTACAGG
    GACATGAAAG GAATCAGCTT TACAGTAGTG GATGAAGGGA AGCCAATCCA TGTTCCCCAA
    GTTCAGTACC ATGGGAACAT CTTCTTCTGG AACCAGTCCC GTAATAAGCA TGATTATAAT
    GGTTCATTCC TGGCTCTGCC TCTTCAAGAT GCATATATGA GGATCTTTGG GGTCTTGGCT
    GTTGATACCC TTAGAGATCC CCACGAAATA AACATCTTTC TACCTCATGA GATCAGATTC
    TATCAGGGTG TTGCTAATGT CTTTAGCACT GCCTATCACT ACGTCCACAG CCGGGAGCAC
    ATTCTGCATA TTGTGATCAC TGGCATAGGC TGGCTTTATG ACGTCACATC CAGCATCACC
    TCCATCACTA CGTACTTTGT AGAGCCTAGC CCAGCCCAGG ATTCAGACTA TGTTTTACGC
    AACATGATGG TTACAGGGCA GCTGGGTCTA ACAGAAATCC ACAAAAATCC TCCTACCATC
    CACAGGAAGT CATGCATCTT CAGAGATTTC CTCTTTAAAT GTACTGACAG TTCAGAAGTT
    GTTCTGGCTT CTGCCTGTGG AGAAACGCAT ATAGTAGTTC CACTTCGTGA GAGAACAGGA
    GAGGCTCTGG GAGTCCTCGA TTTTAACATC GGCCAAAATA GGATGTTGTT GTGTCAAGAA
    TATAAAGATC TACAGAAAAT GATGAAAGTG GTCCAAGTGG CCTGCTATGA AATACTTGGC
    GAGTTCTCTG GAGAGATAAA GAAAAAATAT ATCTTAGAGA TTGAAAATGT CAGGGAAGTC
    CAGCGGGCAG GAATTCTCTT CTTCCGAATC ATGCTGCTCG AGCTACAGGA AAGCATCCAA
    CTACTCAATT CCATGGAATT TGTGTCACTG TTGCTCTATG ACCATACTCT TGTAACAGAG
    CCAAATTCTC CTCAAGACAG CAAATCTATG GAGTTGGAAG CCAACGTGAA ACTAGTGCGT
    GACATCCTGA AGGCGGTTAT CTTGTTCTTT CATCCAGAGT TGGAATTTTC AAGTGACTTT
    GGAAGTTGGG ATAAGTGTAA ATTTTATGTT AACAAATATT TAGTCAACAA TATTTGTGCC
    TTTGATCCAA CTGCCAAGCA TGTGGAAGTT AATGTACAGC TTATTGATGA ATATATCAGA
    GATCATTCCC GAACTGAAGT ATGGAAATTT GGTAATGTTG TCATTGAACA TCTATACCAC
    TGGATACACA TCTGTTCAGC TCTCATGAAG ATAACCAAAC AACTAAATAG TGGTATTACA
    CCTCCGTTGC CCTCCAAGAC TGACAATTAT ATGTATGCAA AAATGCCAGG GGAAGGTTTG
    CAAGAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_940931.2
    CDS193..4704
    Translation

    Target ORF information:

    RefSeq Version NM_198529.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198529.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CAAAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGATTCAG AATGTTCTTC AAGAATTCTT TCAAAATCCA
    GATTTCAAGC TTGGATCACA CTGCAAACAA CTGGATATTA CTGACTCAAC AGAACCAAGA
    TTGAACAAAA TGGAATTTAC AGAATACATC TCTTCACATA TTAAAGACTT GAAGAGTGAA
    ATGTTTGAGG AACTTCTTAA GCACCTTTGC CACTCTGCAG ATGAATTTCG GGAGGTCATA
    AAAGCTGACA TGCGGAGGCA GATGTTCGCT GAACTCTTCC TACATTGTGA CCACGGGAAG
    GTAGGGTTTT TGGATCGGCA GAGGACATTG GCCCTGCTGG AATTGTTCTA TGACCATAGT
    TCACAAATGC TTAGGAGTTT ACTTCGAAAC CCACGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAAACT TGACTGAGTT GTGGGGAGAC ATGGATAATC AGAAACACAT TTATGAAGGT
    TTTGACAAAG TGCTCTTGGA GATGAATACA TTACTTTCTG CAAATCATGC TAGCAAAACC
    CAAAGTAAAT TATTAGAAAG TCCAGATCAA CCTAAACTTA ACGAACAGAG AACATCAACA
    CCATCACCAA ACCCGCCAGA ACAGCAGAGA GGAGTAACTG CAGAACAAGG ACCACAAAGA
    ATTTCAATTG AAGAACAACA ACAAGGCAAA AAGCCAACTG CAGAGCAAGA ACTGTACATA
    GAATCAGTAA TAGAACCAGG AACACACACA GAGTCAACTC TAGAACAAGG GTCAAGTAGA
    AGGTTACTGA CAGAACAAGA AACACACAGA GAGTCAACTA CAGAACAAGG ACAGCACAAA
    GGGTCAATAG AAGGACAAGG ACCACGCAGA GTGTCAGTTT CAGAACAAGG ATCAAGCAGA
    GAGTCAGTTG CAGAACAAGG GTCACGCAGA GAGTCTATTG CAGAACAAGA TCGACACAAA
    GGGTCAGTAG CAGAACAAGG ATCACGCAGA ATGTCAGCTG CAGAACAGGG ATCACTCAGA
    GAGTCAGTAA TAGAAGAACC ATACCAAAAA TCAGAACAAG GACCTTATGG AGAGATAATT
    TCAGAAGAGC AAGAAGACAT AGGCTCAACT TCACAATCAA GAAAAGATAG TATCTTAAAA
    AGTACAAAAT ATGGGGAACC TATAACCTCT GAGTACATTG AAGTCCCTCT ACAGGAAAAG
    AGGTCTTGGG AACAAACATA TGAAGAGGAA ATATTCCTGA GTTCTGAACT GCAAGAGGAA
    GTTCCAACCT TAAGCAGAAA AGATCACTTT CCAGAAACTA CAAAAAAGGA AGTTCAGAAA
    GACAAGCCCT GTGAACCCAA GTCCCAAAAA ATAGAAGGAA AGTCATGGTC AGGTGAATTT
    TTTACTTGTA ACTGGAAAAT GAAGTATGTC ACATTTGAAG ATGAGGAACA GGCAAACTTA
    ATCTATGGTA ACTCCAGGTT CACAGATTTA CACTCAATTA TCAGAAATAT TCAGTCTTGC
    AAGGAGGTAA AAGGCAGAAC TGCCTTCAAT GGAGTTTCAT TCAATCTGCT CCAGTTTGTG
    CAACTCCTGG AGACATTTGT TGGTGAAGAC GCTCCCTTGA GTGTCAGCGA GACTCTCACC
    TCCTTTTTTA AGGAGGGCTA TGTTGAAACA GAACAAGAGA AAATGAATGC CTTAGAACAG
    TTTAGCCAAA ATGCTTTCCA AGTCCGACAG AGGCTTCTCC TAGAAGCCAT CTTTCAGAAG
    TGGGACAGTG ATGGCTCAGG CTTCCTGGAT CTGAAGGAAG TTGATGAACT CTTGTACACA
    TACAAGGAGG GAATGGAAAA AGAATCTATG AAGAAAGCTA AACTACACAT CCAATTTCCA
    AAGCCACACC CTGGTCACGA AGTGAGATTG TCTTCAAAAC AATTTCAGAA TTACATAGAA
    TTGGTTGTGT CTGAACTCAG GGGCAATGAA GACCAAGTTC TGGAAAGTGT TGTGGAATTT
    CTGATGAATG CTTTAGAGAG GAGCCACATT GAGAGTCTGA GGAATTCTGC CAGGCGGAAA
    TGGCTGCACC AAATCCAATG TGCTGCAGAG ACAAGTGGGG TGTCCCTAGA GCCGGTGTAT
    AGTGAGACCT TTAAGGCCCT CATGCAGGAT GCTGAAGCCC ATGGAAATAA AAAGATCAGT
    GCTCACATTT CCCTCTTAGA AGAAAACCTA CTACTGCCTG AGAAAGGGAA TGTTCTATTG
    AGGAATGTGG CTTGTACCTT AGATGATGCT CAATTTGTAC TGAACAGAGT GCTCTACAGG
    GACATGAAAG GAATCAGCTT TACAGTAGTG GATGAAGGGA AGCCAATCCA TGTTCCCCAA
    GTTCAGTACC ATGGGAACAT CTTCTTCTGG AACCAGTCCC GTAATAAGCA TGATTATAAT
    GGTTCATTCC TGGCTCTGCC TCTTCAAGAT GCATATATGA GGATCTTTGG GGTCTTGGCT
    GTTGATACCC TTAGAGATCC CCACGAAATA AACATCTTTC TACCTCATGA GATCAGATTC
    TATCAGGGTG TTGCTAATGT CTTTAGCACT GCCTATCACT ACGTCCACAG CCGGGAGCAC
    ATTCTGCATA TTGTGATCAC TGGCATAGGC TGGCTTTATG ACGTCACATC CAGCATCACC
    TCCATCACTA CGTACTTTGT AGAGCCTAGC CCAGCCCAGG ATTCAGACTA TGTTTTACGC
    AACATGATGG TTACAGGGCA GCTGGGTCTA ACAGAAATCC ACAAAAATCC TCCTACCATC
    CACAGGAAGT CATGCATCTT CAGAGATTTC CTCTTTAAAT GTACTGACAG TTCAGAAGTT
    GTTCTGGCTT CTGCCTGTGG AGAAACGCAT ATAGTAGTTC CACTTCGTGA GAGAACAGGA
    GAGGCTCTGG GAGTCCTCGA TTTTAACATC GGCCAAAATA GGATGTTGTT GTGTCAAGAA
    TATAAAGATC TACAGAAAAT GATGAAAGTG GTCCAAGTGG CCTGCTATGA AATACTTGGC
    GAGTTCTCTG GAGAGATAAA GAAAAAATAT ATCTTAGAGA TTGAAAATGT CAGGGAAGTC
    CAGCGGGCAG GAATTCTCTT CTTCCGAATC ATGCTGCTCG AGCTACAGGA AAGCATCCAA
    CTACTCAATT CCATGGAATT TGTGTCACTG TTGCTCTATG ACCATACTCT TGTAACAGAG
    CCAAATTCTC CTCAAGACAG CAAATCTATG GAGTTGGAAG CCAACGTGAA ACTAGTGCGT
    GACATCCTGA AGGCGGTTAT CTTGTTCTTT CATCCAGAGT TGGAATTTTC AAGTGACTTT
    GGAAGTTGGG ATAAGTGTAA ATTTTATGTT AACAAATATT TAGTCAACAA TATTTGTGCC
    TTTGATCCAA CTGCCAAGCA TGTGGAAGTT AATGTACAGC TTATTGATGA ATATATCAGA
    GATCATTCCC GAACTGAAGT ATGGAAATTT GGTAATGTTG TCATTGAACA TCTATACCAC
    TGGATACACA TCTGTTCAGC TCTCATGAAG ATAACCAAAC AACTAAATAG TGGTATTACA
    CCTCCGTTGC CCTCCAAGAC TGACAATTAT ATGTATGCAA AAATGCCAGG GGAAGGTTTG
    CAAGAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108319
    Clone ID Related Accession (Same CDS sequence) NM_001145053.2
    Accession Version NM_001145053.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104982.7 and AC104996.4. On Jun 2, 2019 this sequence version replaced NM_001145053.1. Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR and coding region compared to variant 1. These differences cause translation initiation at a downstream in-frame AUG and a frame shift at the 3' coding region. The resulting isoform (b) is shorter at both termini and has a distinct C-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK299608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGATGAAA TCAAATCCCA AGAATTAAAC CTGGAGGGGC AGCGAAAAAT TTCACCTGGT 
    TCAATAAAGG ACTCTAAAAC TGAAGCCTCA GGTAATATTG CAATTAGAAA ATCTGCAAAA
    GTGATTTTTG CTTTAGATGA AACTGAACTG AAATCAAAGC CAGAGCACAC ATGGAAGAAA
    AACCTTTTTG AAAGAATGGA GGCAAGAGCC CAAGCAATGC AGCAGAAAAT AATAGATAAG
    GAAAATCTGA AGAAGGAACT AGAAAAAAAG GCTGAAAAAA AACTCCCTAG GGATAATTTG
    GCCAAAGAGT GGTTTAATAC TGACAGCATG ACACTGAATA ATACTGCATA TTTGCTTGAC
    AAACTTCTAC CCACCTTAGT TCCTGGAGTA GAAAACATGT TAACTCAAGT AGAAAAGAAG
    AAGGTTTTGA CAGAAGCTGA TACTCCAAGC AAGTTTGACC CAATTAATTA TTTGGGGGAA
    TATTTAATAA GAAACAATCC TAATTATATC AAAGACCCAG GAATGTCTGG TTACCAGAGG
    TTGATGAAAG AAGTCACAGA AGACCTGAAG ATATATGTTC CTGACACTAT CTGCAACAGA
    GTATCCAAGA TGAAGGAGAA TGTTAAACAG AATAGAAAAC AAAGAGAAAG CATAGACAAA
    ATCATAGTCA AGGTGGCCAA CACACGGAAA CAGGCTCTGC AGGAGCAATT CGATGAATGG
    ATTCTAGACC CTAAAGGAAT GATTCCTAAG TCAGTGATTC AGAATGTTCT TCAAGAATTC
    TTTCAAAATC CAGATTTCAA GCTTGGATCA CACTGCAAAC AACTGGATAT TACTGACTCA
    ACAGAACCAA GATTGAACAA AATGGAATTT ACAGAATACA TCTCTTCACA TATTAAAGAC
    TTGAAGAGTG AAATGTTTGA GGAACTTCTT AAGCACCTTT GCCACTCTGC AGATGAATTT
    CGGGAGGTCA TAAAAGCTGA CATGCGGAGG CAGATGTTCG CTGAACTCTT CCTACATTGT
    GACCACGGGA AGGTAGGGTT TTTGGATCGG CAGAGGACAT TGGCCCTGCT GGAATTGTTC
    TATGACCATA GTTCACAAAT GCTTAGGAGT TTACTTCGAA ACCCACGACA ATGGCCATTC
    ATTGAATTTG AAGAGATAAA CTTGACTGAG TTGTGGGGAG ACATGGATAA TCAGAAACAC
    ATTTATGAAG GTTTTGACAA AGTGCTCTTG GAGATGAATA CATTACTTTC TGCAAATCAT
    GCTAGCAAAA CCCAAAGTAA ATTATTAGAA AGTCCAGATC AACCTAAACT TAACGAACAG
    AGAACATCAA CACCATCACC AAACCCGCCA GAACAGCAGA GAGGAGTAAC TGCAGAACAA
    GGACCACAAA GAATTTCAAT TGAAGAACAA CAACAAGGCA AAAAGCCAAC TGCAGAGCAA
    GAACTGTACA TAGAATCAGT AATAGAACCA GGAACACACA CAGAGTCAAC TCTAGAACAA
    GGGTCAAGTA GAAGGTTACT GACAGAACAA GAAACACACA GAGAGTCAAC TACAGAACAA
    GGACAGCACA AAGGGTCAAT AGAAGGACAA GGACCACGCA GAGTGTCAGT TTCAGAACAA
    GGATCAAGCA GAGAGTCAGT TGCAGAACAA GGGTCACGCA GAGAGTCTAT TGCAGAACAA
    GATCGACACA AAGGGTCAGT AGCAGAACAA GGATCACGCA GAATGTCAGC TGCAGAACAG
    GGATCACTCA GAGAGTCAGT AATAGAAGAA CCATACCAAA AATCAGAACA AGGACCTTAT
    GGAGAGATAA TTTCAGAAGA GCAAGAAGAC ATAGGCTCAA CTTCACAATC AAGAAAAGAT
    AGTATCTTAA AAAGTACAAA ATATGGGGAA CCTATAACCT CTGAGTACAT TGAAGTCCCT
    CTACAGGAAA AGAGGTCTTG GGAACAAACA TATGAAGAGG AAATATTCCT GAGTTCTGAA
    CTGCAAGAGG AAGTTCCAAC CTTAAGCAGA AAAGATCACT TTCCAGAAAC TACAAAAAAG
    GAAGTTCAGA AAGACAAGCC CTGTGAACCC AAGTCCCAAA AAATAGAAGG AAAGTCATGG
    TCAGGTGAAT TTTTTACTTG TAACTGGAAA ATGAAGTATG TCACATTTGA AGATGAGGAA
    CAGGCAAACT TAATCTATGG TAACTCCAGG TTCACAGATT TACACTCAAT TATCAGAAAT
    ATTCAGTCTT GCAAGGAGGT AAAAGGCAGA ACTGCCTTCA ATGGAGTTTC ATTCAATCTG
    CTCCAGTTTG TGCAACTCCT GGAGACATTT GTTGGTGAAG ACGCTCCCTT GAGTGTCAGC
    GAGACTCTCA CCTCCTTTTT TAAGGAGGGC TATGTTGAAA CAGAACAAGA GAAAATGAAT
    GCCTTAGAAC AGGTCTCACT CTGTCGCCAG GCTGGAGTGC AGTGGCGCAA CTGTAGCTCA
    CTGCAGCCTC CAACTCCCGG ACTCAAGCGA TCATCCCATC ACAGACTCCT GAGCAACTGG
    GACTACAGGG AAAGAAGGAA GTTTTTTCCT CTTGAGACCA AATTTCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138525.2
    CDS193..2763
    Translation

    Target ORF information:

    RefSeq Version NM_001145053.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145053.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGATGAAA TCAAATCCCA AGAATTAAAC CTGGAGGGGC AGCGAAAAAT TTCACCTGGT 
    TCAATAAAGG ACTCTAAAAC TGAAGCCTCA GGTAATATTG CAATTAGAAA ATCTGCAAAA
    GTGATTTTTG CTTTAGATGA AACTGAACTG AAATCAAAGC CAGAGCACAC ATGGAAGAAA
    AACCTTTTTG AAAGAATGGA GGCAAGAGCC CAAGCAATGC AGCAGAAAAT AATAGATAAG
    GAAAATCTGA AGAAGGAACT AGAAAAAAAG GCTGAAAAAA AACTCCCTAG GGATAATTTG
    GCCAAAGAGT GGTTTAATAC TGACAGCATG ACACTGAATA ATACTGCATA TTTGCTTGAC
    AAACTTCTAC CCACCTTAGT TCCTGGAGTA GAAAACATGT TAACTCAAGT AGAAAAGAAG
    AAGGTTTTGA CAGAAGCTGA TACTCCAAGC AAGTTTGACC CAATTAATTA TTTGGGGGAA
    TATTTAATAA GAAACAATCC TAATTATATC AAAGACCCAG GAATGTCTGG TTACCAGAGG
    TTGATGAAAG AAGTCACAGA AGACCTGAAG ATATATGTTC CTGACACTAT CTGCAACAGA
    GTATCCAAGA TGAAGGAGAA TGTTAAACAG AATAGAAAAC AAAGAGAAAG CATAGACAAA
    ATCATAGTCA AGGTGGCCAA CACACGGAAA CAGGCTCTGC AGGAGCAATT CGATGAATGG
    ATTCTAGACC CTAAAGGAAT GATTCCTAAG TCAGTGATTC AGAATGTTCT TCAAGAATTC
    TTTCAAAATC CAGATTTCAA GCTTGGATCA CACTGCAAAC AACTGGATAT TACTGACTCA
    ACAGAACCAA GATTGAACAA AATGGAATTT ACAGAATACA TCTCTTCACA TATTAAAGAC
    TTGAAGAGTG AAATGTTTGA GGAACTTCTT AAGCACCTTT GCCACTCTGC AGATGAATTT
    CGGGAGGTCA TAAAAGCTGA CATGCGGAGG CAGATGTTCG CTGAACTCTT CCTACATTGT
    GACCACGGGA AGGTAGGGTT TTTGGATCGG CAGAGGACAT TGGCCCTGCT GGAATTGTTC
    TATGACCATA GTTCACAAAT GCTTAGGAGT TTACTTCGAA ACCCACGACA ATGGCCATTC
    ATTGAATTTG AAGAGATAAA CTTGACTGAG TTGTGGGGAG ACATGGATAA TCAGAAACAC
    ATTTATGAAG GTTTTGACAA AGTGCTCTTG GAGATGAATA CATTACTTTC TGCAAATCAT
    GCTAGCAAAA CCCAAAGTAA ATTATTAGAA AGTCCAGATC AACCTAAACT TAACGAACAG
    AGAACATCAA CACCATCACC AAACCCGCCA GAACAGCAGA GAGGAGTAAC TGCAGAACAA
    GGACCACAAA GAATTTCAAT TGAAGAACAA CAACAAGGCA AAAAGCCAAC TGCAGAGCAA
    GAACTGTACA TAGAATCAGT AATAGAACCA GGAACACACA CAGAGTCAAC TCTAGAACAA
    GGGTCAAGTA GAAGGTTACT GACAGAACAA GAAACACACA GAGAGTCAAC TACAGAACAA
    GGACAGCACA AAGGGTCAAT AGAAGGACAA GGACCACGCA GAGTGTCAGT TTCAGAACAA
    GGATCAAGCA GAGAGTCAGT TGCAGAACAA GGGTCACGCA GAGAGTCTAT TGCAGAACAA
    GATCGACACA AAGGGTCAGT AGCAGAACAA GGATCACGCA GAATGTCAGC TGCAGAACAG
    GGATCACTCA GAGAGTCAGT AATAGAAGAA CCATACCAAA AATCAGAACA AGGACCTTAT
    GGAGAGATAA TTTCAGAAGA GCAAGAAGAC ATAGGCTCAA CTTCACAATC AAGAAAAGAT
    AGTATCTTAA AAAGTACAAA ATATGGGGAA CCTATAACCT CTGAGTACAT TGAAGTCCCT
    CTACAGGAAA AGAGGTCTTG GGAACAAACA TATGAAGAGG AAATATTCCT GAGTTCTGAA
    CTGCAAGAGG AAGTTCCAAC CTTAAGCAGA AAAGATCACT TTCCAGAAAC TACAAAAAAG
    GAAGTTCAGA AAGACAAGCC CTGTGAACCC AAGTCCCAAA AAATAGAAGG AAAGTCATGG
    TCAGGTGAAT TTTTTACTTG TAACTGGAAA ATGAAGTATG TCACATTTGA AGATGAGGAA
    CAGGCAAACT TAATCTATGG TAACTCCAGG TTCACAGATT TACACTCAAT TATCAGAAAT
    ATTCAGTCTT GCAAGGAGGT AAAAGGCAGA ACTGCCTTCA ATGGAGTTTC ATTCAATCTG
    CTCCAGTTTG TGCAACTCCT GGAGACATTT GTTGGTGAAG ACGCTCCCTT GAGTGTCAGC
    GAGACTCTCA CCTCCTTTTT TAAGGAGGGC TATGTTGAAA CAGAACAAGA GAAAATGAAT
    GCCTTAGAAC AGGTCTCACT CTGTCGCCAG GCTGGAGTGC AGTGGCGCAA CTGTAGCTCA
    CTGCAGCCTC CAACTCCCGG ACTCAAGCGA TCATCCCATC ACAGACTCCT GAGCAACTGG
    GACTACAGGG AAAGAAGGAA GTTTTTTCCT CTTGAGACCA AATTTCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104399
    Clone ID Related Accession (Same CDS sequence) XM_024450742.1
    Accession Version XM_024450742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATGAAA TCAAATCCCA AGAATTAAAC CTGGAGGGGC AGCGAAAAAT TTCACCTGGT 
    TCAATAAAGG ACTCTAAAAC TGAAGCCTCA GGTAATATTG CAATTAGAAA ATCTGCAAAA
    GTGATTTTTG CTTTAGATGA AACTGAACTG AAATCAAAGC CAGAGCACAC ATGGAAGAAA
    AACCTTTTTG AAAGAATGGA GGCAAGAGCC CAAGCAATGC AGCAGAAAAT AATAGATAAG
    GAAAATCTGA AGAAGGAACT AGAAAAAAAG GCTGAAAAAA AACTCCCTAG GGATAATTTG
    GCCAAAGAGT GGTTTAATAC TGACAGCATG ACACTGAATA ATACTGCATA TTTGCTTGAC
    AAACTTCTAC CCACCTTAGT TCCTGGAGTA GAAAACATGT TAACTCAAGT AGAAAAGAAG
    AAGGTTTTGA CAGAAGCTGA TACTCCAAGC AAGTTTGACC CAATTAATTA TTTGGGGGAA
    TATTTAATAA GAAACAATCC TAATTATATC AAAGACCCAG GAATGTCTGG TTACCAGAGG
    TTGATGAAAG AAGTCACAGA AGACCTGAAG ATATATGTTC CTGACACTAT CTGCAACAGA
    GTATCCAAGA TGAAGGAGAA TGTTAAACAG AATAGAAAAC AAAGAGAAAG CATAGACAAA
    ATCATAGTCA AGGTGGCCAA CACACGGAAA CAGGCTCTGC AGGAGCAATT CGATGAATGG
    ATTCTAGACC CTAAAGGAAT GATTCCTAAG TCAGTGTTGC AGATTCAGAA TGTTCTTCAA
    GAATTCTTTC AAAATCCAGA TTTCAAGCTT GGATCACACT GCAAACAACT GGATATTACT
    GACTCAACAG AACCAAGATT GAACAAAATG GAATTTACAG AATACATCTC TTCACATATT
    AAAGACTTGA AGAGTGAAAT GTTTGAGGAA CTTCTTAAGC ACCTTTGCCA CTCTGCAGAT
    GAATTTCGGG AGGTCATAAA AGCTGACATG CGGAGGCAGA TGTTCGCTGA ACTCTTCCTA
    CATTGTGACC ACGGGAAGGT AGGGTTTTTG GATCGGCAGA GGACATTGGC CCTGCTGGAA
    TTGTTCTATG ACCATAGTTC ACAAATGCTT AGGAGTTTAC TTCGAAACCC ACGACAATGG
    CCATTCATTG AATTTGAAGA GATAAACTTG ACTGAGTTGT GGGGAGACAT GGATAATCAG
    AAACACATTT ATGAAGGTTT TGACAAAGTG CTCTTGGAGA TGAATACATT ACTTTCTGCA
    AATCATGCTA GCAAAACCCA AAGTAAATTA TTAGAAAGTC CAGATCAACC TAAACTTAAC
    GAACAGAGAA CATCAACACC ATCACCAAAC CCGCCAGAAC AGCAGAGAGG AGTAACTGCA
    GAACAAGGAC CACAAAGAAT TTCAATTGAA GAACAACAAC AAGGCAAAAA GCCAACTGCA
    GAGCAAGAAC TGTACATAGA ATCAGTAATA GAACCAGGAA CACACACAGA GTCAACTCTA
    GAACAAGGGT CAAGTAGAAG GTTACTGACA GAACAAGAAA CACACAGAGA GTCAACTACA
    GAACAAGGAC AGCACAAAGG GTCAATAGAA GGACAAGGAC CACGCAGAGT GTCAGTTTCA
    GAACAAGGAT CAAGCAGAGA GTCAGTTGCA GAACAAGGGT CACGCAGAGA GTCTATTGCA
    GAACAAGATC GACACAAAGG GTCAGTAGCA GAACAAGGAT CACGCAGAAT GTCAGCTGCA
    GAACAGGGAT CACTCAGAGA GTCAGTAATA GAAGAACCAT ACCAAAAATC AGAACAAGGA
    CCTTATGGAG AGATAATTTC AGAAGAGCAA GAAGACATAG GCTCAACTTC ACAATCAAGA
    AAAGATAGTA TCTTAAAAAG TACAAAATAT GGGGAACCTA TAACCTCTGA GTACATTGAA
    GTCCCTCTAC AGGAAAAGAG GTCTTGGGAA CAAACATATG AAGAGGAAAT ATTCCTGAGT
    TCTGAACTGC AAGAGGAAGT TCCAACCTTA AGCAGAAAAG ATCACTTTCC AGAAACTACA
    AAAAAGGAAG TTCAGAAAGA CAAGCCCTGT GAACCCAAGT CCCAAAAAAT AGAAGGAAAG
    TCATGGTCAG GTGAATTTTT TACTTGTAAC TGGAAAATGA AGTATGTCAC ATTTGAAGAT
    GAGGAACAGG CAAACTTAAT CTATGGTAAC TCCAGGTTCA CAGATTTACA CTCAATTATC
    AGAAATATTC AGTCTTGCAA GGAGGTAAAA GGCAGAACTG CCTTCAATGG AGTTTCATTC
    AATCTGCTCC AGTTTGTGCA ACTCCTGGAG ACATTTGTTG GTGAAGACGC TCCCTTGAGT
    GTCAGCGAGA CTCTCACCTC CTTTTTTAAG GAGGGCTATG TTGAAACAGA ACAAGAGAAA
    ATGAATGCCT TAGAACAGTT TAGCCAAAAT GCTTTCCAAG TCCGACAGAG GCTTCTCCTA
    GAAGCCATCT TTCAGAAGTG GGACAGTGAT GGCTCAGGCT TCCTGGATCT GAAGGAAGTT
    GATGAACTCT TGTACACATA CAAGGAGGGA ATGGAAAAAG AATCTATGAA GAAAGCTAAA
    CTACACATCC AATTTCCAAA GCCACACCCT GGTCACGAAG TGAGATTGTC TTCAAAACAA
    TTTCAGAATT ACATAGAATT GGTTGTGTCT GAACTCAGGG GCAATGAAGA CCAAGTTCTG
    GAAAGTGTTG TGGAATTTCT GATGAATGCT TTAGAGAGGA GCCACATTGA GAGTCTGAGG
    AATTCTGCCA GGCGGAAATG GCTGCACCAA ATCCAATGTG CTGCAGAGAC AAGTGGGGTG
    TCCCTAGAGC CGGTGTATAG TGAGACCTTT AAGGCCCTCA TGCAGGATGC TGAAGCCCAT
    GGAAATAAAA AGATCAGTGC TCACATTTCC CTCTTAGAAG AAAACCTACT ACTGCCTGAG
    AAAGGGAATG TTCTATTGAG GAATGTGGCT TGTACCTTAG ATGATGCTCA ATTTGTACTG
    AACAGAGTGC TCTACAGGGA CATGAAAGGA ATCAGCTTTA CAGTAGTGGA TGAAGGGAAG
    CCAATCCATG TTCCCCAAGT TCAGTACCAT GGGAACATCT TCTTCTGGAA CCAGTCCCGT
    AATAAGCATG ATTATAATGG TTCATTCCTG GCTCTGCCTC TTCAAGATGC ATATATGAGG
    ATCTTTGGGG TCTTGGCTGT TGATACCCTT AGAGATCCCC ACGAAATAAA CATCTTTCTA
    CCTCATGAGA TCAGATTCTA TCAGGGTGTT GCTAATGTCT TTAGCACTGC CTATCACTAC
    GTCCACAGCC GGGAGCACAT TCTGCATATT GTGATCACTG GCATAGGCTG GCTTTATGAC
    GTCACATCCA GCATCACCTC CATCACTACG TACTTTGTAG AGCCTAGCCC AGCCCAGGAT
    TCAGACTATG TTTTACGCAA CATGATGGTT ACAGGGCAGC TGGGTCTAAC AGAAATCCAC
    AAAAATCCTC CTACCATCCA CAGGAAGTCA TGCATCTTCA GAGATTTCCT CTTTAAATGT
    ACTGACAGTT CAGAAGTTGT TCTGGCTTCT GCCTGTGGAG AAACGCATAT AGTAGTTCCA
    CTTCGTGAGA GAACAGGAGA GGCTCTGGGA GTCCTCGATT TTAACATCGG CCAAAATAGG
    ATGTTGTTGT GTCAAGAATA TAAAGATCTA CAGAAAATGA TGAAAGTGGT CCAAGTGGCC
    TGCTATGAAA TACTTGGCGA GTTCTCTGGA GAGATAAAGA AAAAATATAT CTTAGAGATT
    GAAAATGTCA GGGAAGTCCA GCGGGCAGGA ATTCTCTTCT TCCGAATCAT GCTGCTCGAG
    CTACAGGAAA GCATCCAACT ACTCAATTCC ATGGAATTTG TGTCACTGTT GCTCTATGAC
    CATACTCTTG TAACAGAGCC AAATTCTCCT CAAGACAGCA AATCTATGGA GTTGGAAGCC
    AACGTGAAAC TAGTGCGTGA CATCCTGAAG GCGGTTATCT TGTTCTTTCA TCCAGAGTTG
    GAATTTTCAA GTGACTTTGG AAGTTGGGAT AAGTGTAAAT TTTATGTTAA CAAATATTTA
    GTCAACAATA TTTGTGCCTT TGATCCAACT GCCAAGCATG TGGAAGTTAA TGTACAGCTT
    ATTGATGAAT ATATCAGAGA TCATTCCCGA ACTGAAGTAT GGAAATTTGG TAATGTTGTC
    ATTGAACATC TATACCACTG GATACACATC TGTTCAGCTC TCATGAAGAT AACCAAACAA
    CTAAATAGTG GTATTACACC TCCGTTGCCC TCCAAGACTG ACAATTATAT GTATGCAAAA
    ATGCCAGGGG AAGGTTTGCA AGAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306510.1
    CDS193..4542
    Translation

    Target ORF information:

    RefSeq Version XM_024450742.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATGAAA TCAAATCCCA AGAATTAAAC CTGGAGGGGC AGCGAAAAAT TTCACCTGGT 
    TCAATAAAGG ACTCTAAAAC TGAAGCCTCA GGTAATATTG CAATTAGAAA ATCTGCAAAA
    GTGATTTTTG CTTTAGATGA AACTGAACTG AAATCAAAGC CAGAGCACAC ATGGAAGAAA
    AACCTTTTTG AAAGAATGGA GGCAAGAGCC CAAGCAATGC AGCAGAAAAT AATAGATAAG
    GAAAATCTGA AGAAGGAACT AGAAAAAAAG GCTGAAAAAA AACTCCCTAG GGATAATTTG
    GCCAAAGAGT GGTTTAATAC TGACAGCATG ACACTGAATA ATACTGCATA TTTGCTTGAC
    AAACTTCTAC CCACCTTAGT TCCTGGAGTA GAAAACATGT TAACTCAAGT AGAAAAGAAG
    AAGGTTTTGA CAGAAGCTGA TACTCCAAGC AAGTTTGACC CAATTAATTA TTTGGGGGAA
    TATTTAATAA GAAACAATCC TAATTATATC AAAGACCCAG GAATGTCTGG TTACCAGAGG
    TTGATGAAAG AAGTCACAGA AGACCTGAAG ATATATGTTC CTGACACTAT CTGCAACAGA
    GTATCCAAGA TGAAGGAGAA TGTTAAACAG AATAGAAAAC AAAGAGAAAG CATAGACAAA
    ATCATAGTCA AGGTGGCCAA CACACGGAAA CAGGCTCTGC AGGAGCAATT CGATGAATGG
    ATTCTAGACC CTAAAGGAAT GATTCCTAAG TCAGTGTTGC AGATTCAGAA TGTTCTTCAA
    GAATTCTTTC AAAATCCAGA TTTCAAGCTT GGATCACACT GCAAACAACT GGATATTACT
    GACTCAACAG AACCAAGATT GAACAAAATG GAATTTACAG AATACATCTC TTCACATATT
    AAAGACTTGA AGAGTGAAAT GTTTGAGGAA CTTCTTAAGC ACCTTTGCCA CTCTGCAGAT
    GAATTTCGGG AGGTCATAAA AGCTGACATG CGGAGGCAGA TGTTCGCTGA ACTCTTCCTA
    CATTGTGACC ACGGGAAGGT AGGGTTTTTG GATCGGCAGA GGACATTGGC CCTGCTGGAA
    TTGTTCTATG ACCATAGTTC ACAAATGCTT AGGAGTTTAC TTCGAAACCC ACGACAATGG
    CCATTCATTG AATTTGAAGA GATAAACTTG ACTGAGTTGT GGGGAGACAT GGATAATCAG
    AAACACATTT ATGAAGGTTT TGACAAAGTG CTCTTGGAGA TGAATACATT ACTTTCTGCA
    AATCATGCTA GCAAAACCCA AAGTAAATTA TTAGAAAGTC CAGATCAACC TAAACTTAAC
    GAACAGAGAA CATCAACACC ATCACCAAAC CCGCCAGAAC AGCAGAGAGG AGTAACTGCA
    GAACAAGGAC CACAAAGAAT TTCAATTGAA GAACAACAAC AAGGCAAAAA GCCAACTGCA
    GAGCAAGAAC TGTACATAGA ATCAGTAATA GAACCAGGAA CACACACAGA GTCAACTCTA
    GAACAAGGGT CAAGTAGAAG GTTACTGACA GAACAAGAAA CACACAGAGA GTCAACTACA
    GAACAAGGAC AGCACAAAGG GTCAATAGAA GGACAAGGAC CACGCAGAGT GTCAGTTTCA
    GAACAAGGAT CAAGCAGAGA GTCAGTTGCA GAACAAGGGT CACGCAGAGA GTCTATTGCA
    GAACAAGATC GACACAAAGG GTCAGTAGCA GAACAAGGAT CACGCAGAAT GTCAGCTGCA
    GAACAGGGAT CACTCAGAGA GTCAGTAATA GAAGAACCAT ACCAAAAATC AGAACAAGGA
    CCTTATGGAG AGATAATTTC AGAAGAGCAA GAAGACATAG GCTCAACTTC ACAATCAAGA
    AAAGATAGTA TCTTAAAAAG TACAAAATAT GGGGAACCTA TAACCTCTGA GTACATTGAA
    GTCCCTCTAC AGGAAAAGAG GTCTTGGGAA CAAACATATG AAGAGGAAAT ATTCCTGAGT
    TCTGAACTGC AAGAGGAAGT TCCAACCTTA AGCAGAAAAG ATCACTTTCC AGAAACTACA
    AAAAAGGAAG TTCAGAAAGA CAAGCCCTGT GAACCCAAGT CCCAAAAAAT AGAAGGAAAG
    TCATGGTCAG GTGAATTTTT TACTTGTAAC TGGAAAATGA AGTATGTCAC ATTTGAAGAT
    GAGGAACAGG CAAACTTAAT CTATGGTAAC TCCAGGTTCA CAGATTTACA CTCAATTATC
    AGAAATATTC AGTCTTGCAA GGAGGTAAAA GGCAGAACTG CCTTCAATGG AGTTTCATTC
    AATCTGCTCC AGTTTGTGCA ACTCCTGGAG ACATTTGTTG GTGAAGACGC TCCCTTGAGT
    GTCAGCGAGA CTCTCACCTC CTTTTTTAAG GAGGGCTATG TTGAAACAGA ACAAGAGAAA
    ATGAATGCCT TAGAACAGTT TAGCCAAAAT GCTTTCCAAG TCCGACAGAG GCTTCTCCTA
    GAAGCCATCT TTCAGAAGTG GGACAGTGAT GGCTCAGGCT TCCTGGATCT GAAGGAAGTT
    GATGAACTCT TGTACACATA CAAGGAGGGA ATGGAAAAAG AATCTATGAA GAAAGCTAAA
    CTACACATCC AATTTCCAAA GCCACACCCT GGTCACGAAG TGAGATTGTC TTCAAAACAA
    TTTCAGAATT ACATAGAATT GGTTGTGTCT GAACTCAGGG GCAATGAAGA CCAAGTTCTG
    GAAAGTGTTG TGGAATTTCT GATGAATGCT TTAGAGAGGA GCCACATTGA GAGTCTGAGG
    AATTCTGCCA GGCGGAAATG GCTGCACCAA ATCCAATGTG CTGCAGAGAC AAGTGGGGTG
    TCCCTAGAGC CGGTGTATAG TGAGACCTTT AAGGCCCTCA TGCAGGATGC TGAAGCCCAT
    GGAAATAAAA AGATCAGTGC TCACATTTCC CTCTTAGAAG AAAACCTACT ACTGCCTGAG
    AAAGGGAATG TTCTATTGAG GAATGTGGCT TGTACCTTAG ATGATGCTCA ATTTGTACTG
    AACAGAGTGC TCTACAGGGA CATGAAAGGA ATCAGCTTTA CAGTAGTGGA TGAAGGGAAG
    CCAATCCATG TTCCCCAAGT TCAGTACCAT GGGAACATCT TCTTCTGGAA CCAGTCCCGT
    AATAAGCATG ATTATAATGG TTCATTCCTG GCTCTGCCTC TTCAAGATGC ATATATGAGG
    ATCTTTGGGG TCTTGGCTGT TGATACCCTT AGAGATCCCC ACGAAATAAA CATCTTTCTA
    CCTCATGAGA TCAGATTCTA TCAGGGTGTT GCTAATGTCT TTAGCACTGC CTATCACTAC
    GTCCACAGCC GGGAGCACAT TCTGCATATT GTGATCACTG GCATAGGCTG GCTTTATGAC
    GTCACATCCA GCATCACCTC CATCACTACG TACTTTGTAG AGCCTAGCCC AGCCCAGGAT
    TCAGACTATG TTTTACGCAA CATGATGGTT ACAGGGCAGC TGGGTCTAAC AGAAATCCAC
    AAAAATCCTC CTACCATCCA CAGGAAGTCA TGCATCTTCA GAGATTTCCT CTTTAAATGT
    ACTGACAGTT CAGAAGTTGT TCTGGCTTCT GCCTGTGGAG AAACGCATAT AGTAGTTCCA
    CTTCGTGAGA GAACAGGAGA GGCTCTGGGA GTCCTCGATT TTAACATCGG CCAAAATAGG
    ATGTTGTTGT GTCAAGAATA TAAAGATCTA CAGAAAATGA TGAAAGTGGT CCAAGTGGCC
    TGCTATGAAA TACTTGGCGA GTTCTCTGGA GAGATAAAGA AAAAATATAT CTTAGAGATT
    GAAAATGTCA GGGAAGTCCA GCGGGCAGGA ATTCTCTTCT TCCGAATCAT GCTGCTCGAG
    CTACAGGAAA GCATCCAACT ACTCAATTCC ATGGAATTTG TGTCACTGTT GCTCTATGAC
    CATACTCTTG TAACAGAGCC AAATTCTCCT CAAGACAGCA AATCTATGGA GTTGGAAGCC
    AACGTGAAAC TAGTGCGTGA CATCCTGAAG GCGGTTATCT TGTTCTTTCA TCCAGAGTTG
    GAATTTTCAA GTGACTTTGG AAGTTGGGAT AAGTGTAAAT TTTATGTTAA CAAATATTTA
    GTCAACAATA TTTGTGCCTT TGATCCAACT GCCAAGCATG TGGAAGTTAA TGTACAGCTT
    ATTGATGAAT ATATCAGAGA TCATTCCCGA ACTGAAGTAT GGAAATTTGG TAATGTTGTC
    ATTGAACATC TATACCACTG GATACACATC TGTTCAGCTC TCATGAAGAT AACCAAACAA
    CTAAATAGTG GTATTACACC TCCGTTGCCC TCCAAGACTG ACAATTATAT GTATGCAAAA
    ATGCCAGGGG AAGGTTTGCA AGAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104400
    Clone ID Related Accession (Same CDS sequence) XM_024450743.1
    Accession Version XM_024450743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATGAAA TCAAATCCCA AGAATTAAAC CTGGAGGGGC AGCGAAAAAT TTCACCTGGT 
    TCAATAAAGG ACTCTAAAAC TGAAGCCTCA GGTAATATTG CAATTAGAAA ATCTGCAAAA
    GTGATTTTTG CTTTAGATGA AACTGAACTG AAATCAAAGC CAGAGCACAC ATGGAAGAAA
    AACCTTTTTG AAAGAATGGA GGCAAGAGCC CAAGCAATGC AGCAGAAAAT AATAGATAAG
    GAAAATCTGA AGAAGGAACT AGAAAAAAAG GCTGAAAAAA AACTCCCTAG GGATAATTTG
    GCCAAAGAGT GGTTTAATAC TGACAGCATG ACACTGAATA ATACTGCATA TTTGCTTGAC
    AAACTTCTAC CCACCTTAGT TCCTGGAGTA GAAAACATGT TAACTCAAGT AGAAAAGAAG
    AAGGTTTTGA CAGAAGCTGA TACTCCAAGC AAGTTTGACC CAATTAATTA TTTGGGGGAA
    TATTTAATAA GAAACAATCC TAATTATATC AAAGACCCAG GAATGTCTGG TTACCAGAGG
    TTGATGAAAG AAGTCACAGA AGACCTGAAG ATATATGTTC CTGACACTAT CTGCAACAGA
    GTATCCAAGA TGAAGGAGAA TGTTAAACAG AATAGAAAAC AAAGAGAAAG CATAGACAAA
    ATCATAGTCA AGGTGGCCAA CACACGGAAA CAGGCTCTGC AGGAGCAATT CGATGAATGG
    ATTCTAGACC CTAAAGGAAT GATTCCTAAG TCAGTGATTC AGAATGTTCT TCAAGAATTC
    TTTCAAAATC CAGATTTCAA GCTTGGATCA CACTGCAAAC AACTGGATAT TACTGACTCA
    ACAGAACCAA GATTGAACAA AATGGAATTT ACAGAATACA TCTCTTCACA TATTAAAGAC
    TTGAAGAGTG AAATGTTTGA GGAACTTCTT AAGCACCTTT GCCACTCTGC AGATGAATTT
    CGGGAGGTCA TAAAAGCTGA CATGCGGAGG CAGATGTTCG CTGAACTCTT CCTACATTGT
    GACCACGGGA AGGTAGGGTT TTTGGATCGG CAGAGGACAT TGGCCCTGCT GGAATTGTTC
    TATGACCATA GTTCACAAAT GCTTAGGAGT TTACTTCGAA ACCCACGACA ATGGCCATTC
    ATTGAATTTG AAGAGATAAA CTTGACTGAG TTGTGGGGAG ACATGGATAA TCAGAAACAC
    ATTTATGAAG GTTTTGACAA AGTGCTCTTG GAGATGAATA CATTACTTTC TGCAAATCAT
    GCTAGCAAAA CCCAAAGTAA ATTATTAGAA AGTCCAGATC AACCTAAACT TAACGAACAG
    AGAACATCAA CACCATCACC AAACCCGCCA GAACAGCAGA GAGGAGTAAC TGCAGAACAA
    GGACCACAAA GAATTTCAAT TGAAGAACAA CAACAAGGCA AAAAGCCAAC TGCAGAGCAA
    GAACTGTACA TAGAATCAGT AATAGAACCA GGAACACACA CAGAGTCAAC TCTAGAACAA
    GGGTCAAGTA GAAGGTTACT GACAGAACAA GAAACACACA GAGAGTCAAC TACAGAACAA
    GGACAGCACA AAGGGTCAAT AGAAGGACAA GGACCACGCA GAGTGTCAGT TTCAGAACAA
    GGATCAAGCA GAGAGTCAGT TGCAGAACAA GGGTCACGCA GAGAGTCTAT TGCAGAACAA
    GATCGACACA AAGGGTCAGT AGCAGAACAA GGATCACGCA GAATGTCAGC TGCAGAACAG
    GGATCACTCA GAGAGTCAGT AATAGAAGAA CCATACCAAA AATCAGAACA AGGACCTTAT
    GGAGAGATAA TTTCAGAAGA GCAAGAAGAC ATAGGCTCAA CTTCACAATC AAGAAAAGAT
    AGTATCTTAA AAAGTACAAA ATATGGGGAA CCTATAACCT CTGAGTACAT TGAAGTCCCT
    CTACAGGAAA AGAGGTCTTG GGAACAAACA TATGAAGAGG AAATATTCCT GAGTTCTGAA
    CTGCAAGAGG AAGTTCCAAC CTTAAGCAGA AAAGATCACT TTCCAGAAAC TACAAAAAAG
    GAAGTTCAGA AAGACAAGCC CTGTGAACCC AAGTCCCAAA AAATAGAAGG AAAGTCATGG
    TCAGGTGAAT TTTTTACTTG TAACTGGAAA ATGAAGTATG TCACATTTGA AGATGAGGAA
    CAGGCAAACT TAATCTATGG TAACTCCAGG TTCACAGATT TACACTCAAT TATCAGAAAT
    ATTCAGTCTT GCAAGGAGGT AAAAGGCAGA ACTGCCTTCA ATGGAGTTTC ATTCAATCTG
    CTCCAGTTTG TGCAACTCCT GGAGACATTT GTTGGTGAAG ACGCTCCCTT GAGTGTCAGC
    GAGACTCTCA CCTCCTTTTT TAAGGAGGGC TATGTTGAAA CAGAACAAGA GAAAATGAAT
    GCCTTAGAAC AGTTTAGCCA AAATGCTTTC CAAGTCCGAC AGAGGCTTCT CCTAGAAGCC
    ATCTTTCAGA AGTGGGACAG TGATGGCTCA GGCTTCCTGG ATCTGAAGGA AGTTGATGAA
    CTCTTGTACA CATACAAGGA GGGAATGGAA AAAGAATCTA TGAAGAAAGC TAAACTACAC
    ATCCAATTTC CAAAGCCACA CCCTGGTCAC GAAGTGAGAT TGTCTTCAAA ACAATTTCAG
    AATTACATAG AATTGGTTGT GTCTGAACTC AGGGGCAATG AAGACCAAGT TCTGGAAAGT
    GTTGTGGAAT TTCTGATGAA TGCTTTAGAG AGGAGCCACA TTGAGAGTCT GAGGAATTCT
    GCCAGGCGGA AATGGCTGCA CCAAATCCAA TGTGCTGCAG AGACAAGTGG GGTGTCCCTA
    GAGCCGGTGT ATAGTGAGAC CTTTAAGGCC CTCATGCAGG ATGCTGAAGC CCATGGAAAT
    AAAAAGATCA GTGCTCACAT TTCCCTCTTA GAAGAAAACC TACTACTGCC TGAGAAAGGG
    AATGTTCTAT TGAGGAATGT GGCTTGTACC TTAGATGATG CTCAATTTGT ACTGAACAGA
    GTGCTCTACA GGGACATGAA AGGAATCAGC TTTACAGTAG TGGATGAAGG GAAGCCAATC
    CATGTTCCCC AAGTTCAGTA CCATGGGAAC ATCTTCTTCT GGAACCAGTC CCGTAATAAG
    CATGATTATA ATGGTTCATT CCTGGCTCTG CCTCTTCAAG ATGCATATAT GAGGATCTTT
    GGGGTCTTGG CTGTTGATAC CCTTAGAGAT CCCCACGAAA TAAACATCTT TCTACCTCAT
    GAGATCAGAT TCTATCAGGG TGTTGCTAAT GTCTTTAGCA CTGCCTATCA CTACGTCCAC
    AGCCGGGAGC ACATTCTGCA TATTGTGATC ACTGGCATAG GCTGGCTTTA TGACGTCACA
    TCCAGCATCA CCTCCATCAC TACGTACTTT GTAGAGCCTA GCCCAGCCCA GGATTCAGAC
    TATGTTTTAC GCAACATGAT GGTTACAGGG CAGCTGGGTC TAACAGAAAT CCACAAAAAT
    CCTCCTACCA TCCACAGGAA GTCATGCATC TTCAGAGATT TCCTCTTTAA ATGTACTGAC
    AGTTCAGAAG TTGTTCTGGC TTCTGCCTGT GGAGAAACGC ATATAGTAGT TCCACTTCGT
    GAGAGAACAG GAGAGGCTCT GGGAGTCCTC GATTTTAACA TCGGCCAAAA TAGGATGTTG
    TTGTGTCAAG AATATAAAGA TCTACAGAAA ATGATGAAAG TGGTCCAAGT GGCCTGCTAT
    GAAATACTTG GCGAGTTCTC TGGAGAGATA AAGAAAAAAT ATATCTTAGA GATTGAAAAT
    GTCAGGGAAG TCCAGCGGGC AGGAATTCTC TTCTTCCGAA TCATGCTGCT CGAGCTACAG
    GAAAGCATCC AACTACTCAA TTCCATGGAA TTTGTGTCAC TGTTGCTCTA TGACCATACT
    CTTGTAACAG AGCCAAATTC TCCTCAAGAC AGCAAATCTA TGGAGTTGGA AGCCAACGTG
    AAACTAGTGC GTGACATCCT GAAGGCGGTT ATCTTGTTCT TTCATCCAGA GTTGGAATTT
    TCAAGTGACT TTGGAAGTTG GGATAAGTGT AAATTTTATG TTAACAAATA TTTAGTCAAC
    AATATTTGTG CCTTTGATCC AACTGCCAAG CATGTGGAAG TTAATGTACA GCTTATTGAT
    GAATATATCA GAGATCATTC CCGAACTGAA GTATGGAAAT TTGGTAATGT TGTCATTGAA
    CATCTATACC ACTGGATACA CATCTGTTCA GCTCTCATGA AGATAACCAA ACAACTAAAT
    AGTGGTATTA CACCTCCGTT GCCCTCCAAG ACTGACAATT ATATGTATGC AAAAATGCCA
    GGGGAAGGTT TGCAAGAGAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306511.1
    CDS193..4536
    Translation

    Target ORF information:

    RefSeq Version XM_024450743.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATGAAA TCAAATCCCA AGAATTAAAC CTGGAGGGGC AGCGAAAAAT TTCACCTGGT 
    TCAATAAAGG ACTCTAAAAC TGAAGCCTCA GGTAATATTG CAATTAGAAA ATCTGCAAAA
    GTGATTTTTG CTTTAGATGA AACTGAACTG AAATCAAAGC CAGAGCACAC ATGGAAGAAA
    AACCTTTTTG AAAGAATGGA GGCAAGAGCC CAAGCAATGC AGCAGAAAAT AATAGATAAG
    GAAAATCTGA AGAAGGAACT AGAAAAAAAG GCTGAAAAAA AACTCCCTAG GGATAATTTG
    GCCAAAGAGT GGTTTAATAC TGACAGCATG ACACTGAATA ATACTGCATA TTTGCTTGAC
    AAACTTCTAC CCACCTTAGT TCCTGGAGTA GAAAACATGT TAACTCAAGT AGAAAAGAAG
    AAGGTTTTGA CAGAAGCTGA TACTCCAAGC AAGTTTGACC CAATTAATTA TTTGGGGGAA
    TATTTAATAA GAAACAATCC TAATTATATC AAAGACCCAG GAATGTCTGG TTACCAGAGG
    TTGATGAAAG AAGTCACAGA AGACCTGAAG ATATATGTTC CTGACACTAT CTGCAACAGA
    GTATCCAAGA TGAAGGAGAA TGTTAAACAG AATAGAAAAC AAAGAGAAAG CATAGACAAA
    ATCATAGTCA AGGTGGCCAA CACACGGAAA CAGGCTCTGC AGGAGCAATT CGATGAATGG
    ATTCTAGACC CTAAAGGAAT GATTCCTAAG TCAGTGATTC AGAATGTTCT TCAAGAATTC
    TTTCAAAATC CAGATTTCAA GCTTGGATCA CACTGCAAAC AACTGGATAT TACTGACTCA
    ACAGAACCAA GATTGAACAA AATGGAATTT ACAGAATACA TCTCTTCACA TATTAAAGAC
    TTGAAGAGTG AAATGTTTGA GGAACTTCTT AAGCACCTTT GCCACTCTGC AGATGAATTT
    CGGGAGGTCA TAAAAGCTGA CATGCGGAGG CAGATGTTCG CTGAACTCTT CCTACATTGT
    GACCACGGGA AGGTAGGGTT TTTGGATCGG CAGAGGACAT TGGCCCTGCT GGAATTGTTC
    TATGACCATA GTTCACAAAT GCTTAGGAGT TTACTTCGAA ACCCACGACA ATGGCCATTC
    ATTGAATTTG AAGAGATAAA CTTGACTGAG TTGTGGGGAG ACATGGATAA TCAGAAACAC
    ATTTATGAAG GTTTTGACAA AGTGCTCTTG GAGATGAATA CATTACTTTC TGCAAATCAT
    GCTAGCAAAA CCCAAAGTAA ATTATTAGAA AGTCCAGATC AACCTAAACT TAACGAACAG
    AGAACATCAA CACCATCACC AAACCCGCCA GAACAGCAGA GAGGAGTAAC TGCAGAACAA
    GGACCACAAA GAATTTCAAT TGAAGAACAA CAACAAGGCA AAAAGCCAAC TGCAGAGCAA
    GAACTGTACA TAGAATCAGT AATAGAACCA GGAACACACA CAGAGTCAAC TCTAGAACAA
    GGGTCAAGTA GAAGGTTACT GACAGAACAA GAAACACACA GAGAGTCAAC TACAGAACAA
    GGACAGCACA AAGGGTCAAT AGAAGGACAA GGACCACGCA GAGTGTCAGT TTCAGAACAA
    GGATCAAGCA GAGAGTCAGT TGCAGAACAA GGGTCACGCA GAGAGTCTAT TGCAGAACAA
    GATCGACACA AAGGGTCAGT AGCAGAACAA GGATCACGCA GAATGTCAGC TGCAGAACAG
    GGATCACTCA GAGAGTCAGT AATAGAAGAA CCATACCAAA AATCAGAACA AGGACCTTAT
    GGAGAGATAA TTTCAGAAGA GCAAGAAGAC ATAGGCTCAA CTTCACAATC AAGAAAAGAT
    AGTATCTTAA AAAGTACAAA ATATGGGGAA CCTATAACCT CTGAGTACAT TGAAGTCCCT
    CTACAGGAAA AGAGGTCTTG GGAACAAACA TATGAAGAGG AAATATTCCT GAGTTCTGAA
    CTGCAAGAGG AAGTTCCAAC CTTAAGCAGA AAAGATCACT TTCCAGAAAC TACAAAAAAG
    GAAGTTCAGA AAGACAAGCC CTGTGAACCC AAGTCCCAAA AAATAGAAGG AAAGTCATGG
    TCAGGTGAAT TTTTTACTTG TAACTGGAAA ATGAAGTATG TCACATTTGA AGATGAGGAA
    CAGGCAAACT TAATCTATGG TAACTCCAGG TTCACAGATT TACACTCAAT TATCAGAAAT
    ATTCAGTCTT GCAAGGAGGT AAAAGGCAGA ACTGCCTTCA ATGGAGTTTC ATTCAATCTG
    CTCCAGTTTG TGCAACTCCT GGAGACATTT GTTGGTGAAG ACGCTCCCTT GAGTGTCAGC
    GAGACTCTCA CCTCCTTTTT TAAGGAGGGC TATGTTGAAA CAGAACAAGA GAAAATGAAT
    GCCTTAGAAC AGTTTAGCCA AAATGCTTTC CAAGTCCGAC AGAGGCTTCT CCTAGAAGCC
    ATCTTTCAGA AGTGGGACAG TGATGGCTCA GGCTTCCTGG ATCTGAAGGA AGTTGATGAA
    CTCTTGTACA CATACAAGGA GGGAATGGAA AAAGAATCTA TGAAGAAAGC TAAACTACAC
    ATCCAATTTC CAAAGCCACA CCCTGGTCAC GAAGTGAGAT TGTCTTCAAA ACAATTTCAG
    AATTACATAG AATTGGTTGT GTCTGAACTC AGGGGCAATG AAGACCAAGT TCTGGAAAGT
    GTTGTGGAAT TTCTGATGAA TGCTTTAGAG AGGAGCCACA TTGAGAGTCT GAGGAATTCT
    GCCAGGCGGA AATGGCTGCA CCAAATCCAA TGTGCTGCAG AGACAAGTGG GGTGTCCCTA
    GAGCCGGTGT ATAGTGAGAC CTTTAAGGCC CTCATGCAGG ATGCTGAAGC CCATGGAAAT
    AAAAAGATCA GTGCTCACAT TTCCCTCTTA GAAGAAAACC TACTACTGCC TGAGAAAGGG
    AATGTTCTAT TGAGGAATGT GGCTTGTACC TTAGATGATG CTCAATTTGT ACTGAACAGA
    GTGCTCTACA GGGACATGAA AGGAATCAGC TTTACAGTAG TGGATGAAGG GAAGCCAATC
    CATGTTCCCC AAGTTCAGTA CCATGGGAAC ATCTTCTTCT GGAACCAGTC CCGTAATAAG
    CATGATTATA ATGGTTCATT CCTGGCTCTG CCTCTTCAAG ATGCATATAT GAGGATCTTT
    GGGGTCTTGG CTGTTGATAC CCTTAGAGAT CCCCACGAAA TAAACATCTT TCTACCTCAT
    GAGATCAGAT TCTATCAGGG TGTTGCTAAT GTCTTTAGCA CTGCCTATCA CTACGTCCAC
    AGCCGGGAGC ACATTCTGCA TATTGTGATC ACTGGCATAG GCTGGCTTTA TGACGTCACA
    TCCAGCATCA CCTCCATCAC TACGTACTTT GTAGAGCCTA GCCCAGCCCA GGATTCAGAC
    TATGTTTTAC GCAACATGAT GGTTACAGGG CAGCTGGGTC TAACAGAAAT CCACAAAAAT
    CCTCCTACCA TCCACAGGAA GTCATGCATC TTCAGAGATT TCCTCTTTAA ATGTACTGAC
    AGTTCAGAAG TTGTTCTGGC TTCTGCCTGT GGAGAAACGC ATATAGTAGT TCCACTTCGT
    GAGAGAACAG GAGAGGCTCT GGGAGTCCTC GATTTTAACA TCGGCCAAAA TAGGATGTTG
    TTGTGTCAAG AATATAAAGA TCTACAGAAA ATGATGAAAG TGGTCCAAGT GGCCTGCTAT
    GAAATACTTG GCGAGTTCTC TGGAGAGATA AAGAAAAAAT ATATCTTAGA GATTGAAAAT
    GTCAGGGAAG TCCAGCGGGC AGGAATTCTC TTCTTCCGAA TCATGCTGCT CGAGCTACAG
    GAAAGCATCC AACTACTCAA TTCCATGGAA TTTGTGTCAC TGTTGCTCTA TGACCATACT
    CTTGTAACAG AGCCAAATTC TCCTCAAGAC AGCAAATCTA TGGAGTTGGA AGCCAACGTG
    AAACTAGTGC GTGACATCCT GAAGGCGGTT ATCTTGTTCT TTCATCCAGA GTTGGAATTT
    TCAAGTGACT TTGGAAGTTG GGATAAGTGT AAATTTTATG TTAACAAATA TTTAGTCAAC
    AATATTTGTG CCTTTGATCC AACTGCCAAG CATGTGGAAG TTAATGTACA GCTTATTGAT
    GAATATATCA GAGATCATTC CCGAACTGAA GTATGGAAAT TTGGTAATGT TGTCATTGAA
    CATCTATACC ACTGGATACA CATCTGTTCA GCTCTCATGA AGATAACCAA ACAACTAAAT
    AGTGGTATTA CACCTCCGTT GCCCTCCAAG ACTGACAATT ATATGTATGC AAAAATGCCA
    GGGGAAGGTT TGCAAGAGAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108333
    Clone ID Related Accession (Same CDS sequence) NM_198529.4
    Accession Version NM_198529.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-08
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104982.7, AC104996.4 and AC104984.14. On Jun 2, 2019 this sequence version replaced NM_198529.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC137519.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGATTCAG AATGTTCTTC AAGAATTCTT TCAAAATCCA
    GATTTCAAGC TTGGATCACA CTGCAAACAA CTGGATATTA CTGACTCAAC AGAACCAAGA
    TTGAACAAAA TGGAATTTAC AGAATACATC TCTTCACATA TTAAAGACTT GAAGAGTGAA
    ATGTTTGAGG AACTTCTTAA GCACCTTTGC CACTCTGCAG ATGAATTTCG GGAGGTCATA
    AAAGCTGACA TGCGGAGGCA GATGTTCGCT GAACTCTTCC TACATTGTGA CCACGGGAAG
    GTAGGGTTTT TGGATCGGCA GAGGACATTG GCCCTGCTGG AATTGTTCTA TGACCATAGT
    TCACAAATGC TTAGGAGTTT ACTTCGAAAC CCACGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAAACT TGACTGAGTT GTGGGGAGAC ATGGATAATC AGAAACACAT TTATGAAGGT
    TTTGACAAAG TGCTCTTGGA GATGAATACA TTACTTTCTG CAAATCATGC TAGCAAAACC
    CAAAGTAAAT TATTAGAAAG TCCAGATCAA CCTAAACTTA ACGAACAGAG AACATCAACA
    CCATCACCAA ACCCGCCAGA ACAGCAGAGA GGAGTAACTG CAGAACAAGG ACCACAAAGA
    ATTTCAATTG AAGAACAACA ACAAGGCAAA AAGCCAACTG CAGAGCAAGA ACTGTACATA
    GAATCAGTAA TAGAACCAGG AACACACACA GAGTCAACTC TAGAACAAGG GTCAAGTAGA
    AGGTTACTGA CAGAACAAGA AACACACAGA GAGTCAACTA CAGAACAAGG ACAGCACAAA
    GGGTCAATAG AAGGACAAGG ACCACGCAGA GTGTCAGTTT CAGAACAAGG ATCAAGCAGA
    GAGTCAGTTG CAGAACAAGG GTCACGCAGA GAGTCTATTG CAGAACAAGA TCGACACAAA
    GGGTCAGTAG CAGAACAAGG ATCACGCAGA ATGTCAGCTG CAGAACAGGG ATCACTCAGA
    GAGTCAGTAA TAGAAGAACC ATACCAAAAA TCAGAACAAG GACCTTATGG AGAGATAATT
    TCAGAAGAGC AAGAAGACAT AGGCTCAACT TCACAATCAA GAAAAGATAG TATCTTAAAA
    AGTACAAAAT ATGGGGAACC TATAACCTCT GAGTACATTG AAGTCCCTCT ACAGGAAAAG
    AGGTCTTGGG AACAAACATA TGAAGAGGAA ATATTCCTGA GTTCTGAACT GCAAGAGGAA
    GTTCCAACCT TAAGCAGAAA AGATCACTTT CCAGAAACTA CAAAAAAGGA AGTTCAGAAA
    GACAAGCCCT GTGAACCCAA GTCCCAAAAA ATAGAAGGAA AGTCATGGTC AGGTGAATTT
    TTTACTTGTA ACTGGAAAAT GAAGTATGTC ACATTTGAAG ATGAGGAACA GGCAAACTTA
    ATCTATGGTA ACTCCAGGTT CACAGATTTA CACTCAATTA TCAGAAATAT TCAGTCTTGC
    AAGGAGGTAA AAGGCAGAAC TGCCTTCAAT GGAGTTTCAT TCAATCTGCT CCAGTTTGTG
    CAACTCCTGG AGACATTTGT TGGTGAAGAC GCTCCCTTGA GTGTCAGCGA GACTCTCACC
    TCCTTTTTTA AGGAGGGCTA TGTTGAAACA GAACAAGAGA AAATGAATGC CTTAGAACAG
    TTTAGCCAAA ATGCTTTCCA AGTCCGACAG AGGCTTCTCC TAGAAGCCAT CTTTCAGAAG
    TGGGACAGTG ATGGCTCAGG CTTCCTGGAT CTGAAGGAAG TTGATGAACT CTTGTACACA
    TACAAGGAGG GAATGGAAAA AGAATCTATG AAGAAAGCTA AACTACACAT CCAATTTCCA
    AAGCCACACC CTGGTCACGA AGTGAGATTG TCTTCAAAAC AATTTCAGAA TTACATAGAA
    TTGGTTGTGT CTGAACTCAG GGGCAATGAA GACCAAGTTC TGGAAAGTGT TGTGGAATTT
    CTGATGAATG CTTTAGAGAG GAGCCACATT GAGAGTCTGA GGAATTCTGC CAGGCGGAAA
    TGGCTGCACC AAATCCAATG TGCTGCAGAG ACAAGTGGGG TGTCCCTAGA GCCGGTGTAT
    AGTGAGACCT TTAAGGCCCT CATGCAGGAT GCTGAAGCCC ATGGAAATAA AAAGATCAGT
    GCTCACATTT CCCTCTTAGA AGAAAACCTA CTACTGCCTG AGAAAGGGAA TGTTCTATTG
    AGGAATGTGG CTTGTACCTT AGATGATGCT CAATTTGTAC TGAACAGAGT GCTCTACAGG
    GACATGAAAG GAATCAGCTT TACAGTAGTG GATGAAGGGA AGCCAATCCA TGTTCCCCAA
    GTTCAGTACC ATGGGAACAT CTTCTTCTGG AACCAGTCCC GTAATAAGCA TGATTATAAT
    GGTTCATTCC TGGCTCTGCC TCTTCAAGAT GCATATATGA GGATCTTTGG GGTCTTGGCT
    GTTGATACCC TTAGAGATCC CCACGAAATA AACATCTTTC TACCTCATGA GATCAGATTC
    TATCAGGGTG TTGCTAATGT CTTTAGCACT GCCTATCACT ACGTCCACAG CCGGGAGCAC
    ATTCTGCATA TTGTGATCAC TGGCATAGGC TGGCTTTATG ACGTCACATC CAGCATCACC
    TCCATCACTA CGTACTTTGT AGAGCCTAGC CCAGCCCAGG ATTCAGACTA TGTTTTACGC
    AACATGATGG TTACAGGGCA GCTGGGTCTA ACAGAAATCC ACAAAAATCC TCCTACCATC
    CACAGGAAGT CATGCATCTT CAGAGATTTC CTCTTTAAAT GTACTGACAG TTCAGAAGTT
    GTTCTGGCTT CTGCCTGTGG AGAAACGCAT ATAGTAGTTC CACTTCGTGA GAGAACAGGA
    GAGGCTCTGG GAGTCCTCGA TTTTAACATC GGCCAAAATA GGATGTTGTT GTGTCAAGAA
    TATAAAGATC TACAGAAAAT GATGAAAGTG GTCCAAGTGG CCTGCTATGA AATACTTGGC
    GAGTTCTCTG GAGAGATAAA GAAAAAATAT ATCTTAGAGA TTGAAAATGT CAGGGAAGTC
    CAGCGGGCAG GAATTCTCTT CTTCCGAATC ATGCTGCTCG AGCTACAGGA AAGCATCCAA
    CTACTCAATT CCATGGAATT TGTGTCACTG TTGCTCTATG ACCATACTCT TGTAACAGAG
    CCAAATTCTC CTCAAGACAG CAAATCTATG GAGTTGGAAG CCAACGTGAA ACTAGTGCGT
    GACATCCTGA AGGCGGTTAT CTTGTTCTTT CATCCAGAGT TGGAATTTTC AAGTGACTTT
    GGAAGTTGGG ATAAGTGTAA ATTTTATGTT AACAAATATT TAGTCAACAA TATTTGTGCC
    TTTGATCCAA CTGCCAAGCA TGTGGAAGTT AATGTACAGC TTATTGATGA ATATATCAGA
    GATCATTCCC GAACTGAAGT ATGGAAATTT GGTAATGTTG TCATTGAACA TCTATACCAC
    TGGATACACA TCTGTTCAGC TCTCATGAAG ATAACCAAAC AACTAAATAG TGGTATTACA
    CCTCCGTTGC CCTCCAAGAC TGACAATTAT ATGTATGCAA AAATGCCAGG GGAAGGTTTG
    CAAGAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_940931.3
    CDS164..4675
    Translation

    Target ORF information:

    RefSeq Version NM_198529.4
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198529.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGATTCAG AATGTTCTTC AAGAATTCTT TCAAAATCCA
    GATTTCAAGC TTGGATCACA CTGCAAACAA CTGGATATTA CTGACTCAAC AGAACCAAGA
    TTGAACAAAA TGGAATTTAC AGAATACATC TCTTCACATA TTAAAGACTT GAAGAGTGAA
    ATGTTTGAGG AACTTCTTAA GCACCTTTGC CACTCTGCAG ATGAATTTCG GGAGGTCATA
    AAAGCTGACA TGCGGAGGCA GATGTTCGCT GAACTCTTCC TACATTGTGA CCACGGGAAG
    GTAGGGTTTT TGGATCGGCA GAGGACATTG GCCCTGCTGG AATTGTTCTA TGACCATAGT
    TCACAAATGC TTAGGAGTTT ACTTCGAAAC CCACGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAAACT TGACTGAGTT GTGGGGAGAC ATGGATAATC AGAAACACAT TTATGAAGGT
    TTTGACAAAG TGCTCTTGGA GATGAATACA TTACTTTCTG CAAATCATGC TAGCAAAACC
    CAAAGTAAAT TATTAGAAAG TCCAGATCAA CCTAAACTTA ACGAACAGAG AACATCAACA
    CCATCACCAA ACCCGCCAGA ACAGCAGAGA GGAGTAACTG CAGAACAAGG ACCACAAAGA
    ATTTCAATTG AAGAACAACA ACAAGGCAAA AAGCCAACTG CAGAGCAAGA ACTGTACATA
    GAATCAGTAA TAGAACCAGG AACACACACA GAGTCAACTC TAGAACAAGG GTCAAGTAGA
    AGGTTACTGA CAGAACAAGA AACACACAGA GAGTCAACTA CAGAACAAGG ACAGCACAAA
    GGGTCAATAG AAGGACAAGG ACCACGCAGA GTGTCAGTTT CAGAACAAGG ATCAAGCAGA
    GAGTCAGTTG CAGAACAAGG GTCACGCAGA GAGTCTATTG CAGAACAAGA TCGACACAAA
    GGGTCAGTAG CAGAACAAGG ATCACGCAGA ATGTCAGCTG CAGAACAGGG ATCACTCAGA
    GAGTCAGTAA TAGAAGAACC ATACCAAAAA TCAGAACAAG GACCTTATGG AGAGATAATT
    TCAGAAGAGC AAGAAGACAT AGGCTCAACT TCACAATCAA GAAAAGATAG TATCTTAAAA
    AGTACAAAAT ATGGGGAACC TATAACCTCT GAGTACATTG AAGTCCCTCT ACAGGAAAAG
    AGGTCTTGGG AACAAACATA TGAAGAGGAA ATATTCCTGA GTTCTGAACT GCAAGAGGAA
    GTTCCAACCT TAAGCAGAAA AGATCACTTT CCAGAAACTA CAAAAAAGGA AGTTCAGAAA
    GACAAGCCCT GTGAACCCAA GTCCCAAAAA ATAGAAGGAA AGTCATGGTC AGGTGAATTT
    TTTACTTGTA ACTGGAAAAT GAAGTATGTC ACATTTGAAG ATGAGGAACA GGCAAACTTA
    ATCTATGGTA ACTCCAGGTT CACAGATTTA CACTCAATTA TCAGAAATAT TCAGTCTTGC
    AAGGAGGTAA AAGGCAGAAC TGCCTTCAAT GGAGTTTCAT TCAATCTGCT CCAGTTTGTG
    CAACTCCTGG AGACATTTGT TGGTGAAGAC GCTCCCTTGA GTGTCAGCGA GACTCTCACC
    TCCTTTTTTA AGGAGGGCTA TGTTGAAACA GAACAAGAGA AAATGAATGC CTTAGAACAG
    TTTAGCCAAA ATGCTTTCCA AGTCCGACAG AGGCTTCTCC TAGAAGCCAT CTTTCAGAAG
    TGGGACAGTG ATGGCTCAGG CTTCCTGGAT CTGAAGGAAG TTGATGAACT CTTGTACACA
    TACAAGGAGG GAATGGAAAA AGAATCTATG AAGAAAGCTA AACTACACAT CCAATTTCCA
    AAGCCACACC CTGGTCACGA AGTGAGATTG TCTTCAAAAC AATTTCAGAA TTACATAGAA
    TTGGTTGTGT CTGAACTCAG GGGCAATGAA GACCAAGTTC TGGAAAGTGT TGTGGAATTT
    CTGATGAATG CTTTAGAGAG GAGCCACATT GAGAGTCTGA GGAATTCTGC CAGGCGGAAA
    TGGCTGCACC AAATCCAATG TGCTGCAGAG ACAAGTGGGG TGTCCCTAGA GCCGGTGTAT
    AGTGAGACCT TTAAGGCCCT CATGCAGGAT GCTGAAGCCC ATGGAAATAA AAAGATCAGT
    GCTCACATTT CCCTCTTAGA AGAAAACCTA CTACTGCCTG AGAAAGGGAA TGTTCTATTG
    AGGAATGTGG CTTGTACCTT AGATGATGCT CAATTTGTAC TGAACAGAGT GCTCTACAGG
    GACATGAAAG GAATCAGCTT TACAGTAGTG GATGAAGGGA AGCCAATCCA TGTTCCCCAA
    GTTCAGTACC ATGGGAACAT CTTCTTCTGG AACCAGTCCC GTAATAAGCA TGATTATAAT
    GGTTCATTCC TGGCTCTGCC TCTTCAAGAT GCATATATGA GGATCTTTGG GGTCTTGGCT
    GTTGATACCC TTAGAGATCC CCACGAAATA AACATCTTTC TACCTCATGA GATCAGATTC
    TATCAGGGTG TTGCTAATGT CTTTAGCACT GCCTATCACT ACGTCCACAG CCGGGAGCAC
    ATTCTGCATA TTGTGATCAC TGGCATAGGC TGGCTTTATG ACGTCACATC CAGCATCACC
    TCCATCACTA CGTACTTTGT AGAGCCTAGC CCAGCCCAGG ATTCAGACTA TGTTTTACGC
    AACATGATGG TTACAGGGCA GCTGGGTCTA ACAGAAATCC ACAAAAATCC TCCTACCATC
    CACAGGAAGT CATGCATCTT CAGAGATTTC CTCTTTAAAT GTACTGACAG TTCAGAAGTT
    GTTCTGGCTT CTGCCTGTGG AGAAACGCAT ATAGTAGTTC CACTTCGTGA GAGAACAGGA
    GAGGCTCTGG GAGTCCTCGA TTTTAACATC GGCCAAAATA GGATGTTGTT GTGTCAAGAA
    TATAAAGATC TACAGAAAAT GATGAAAGTG GTCCAAGTGG CCTGCTATGA AATACTTGGC
    GAGTTCTCTG GAGAGATAAA GAAAAAATAT ATCTTAGAGA TTGAAAATGT CAGGGAAGTC
    CAGCGGGCAG GAATTCTCTT CTTCCGAATC ATGCTGCTCG AGCTACAGGA AAGCATCCAA
    CTACTCAATT CCATGGAATT TGTGTCACTG TTGCTCTATG ACCATACTCT TGTAACAGAG
    CCAAATTCTC CTCAAGACAG CAAATCTATG GAGTTGGAAG CCAACGTGAA ACTAGTGCGT
    GACATCCTGA AGGCGGTTAT CTTGTTCTTT CATCCAGAGT TGGAATTTTC AAGTGACTTT
    GGAAGTTGGG ATAAGTGTAA ATTTTATGTT AACAAATATT TAGTCAACAA TATTTGTGCC
    TTTGATCCAA CTGCCAAGCA TGTGGAAGTT AATGTACAGC TTATTGATGA ATATATCAGA
    GATCATTCCC GAACTGAAGT ATGGAAATTT GGTAATGTTG TCATTGAACA TCTATACCAC
    TGGATACACA TCTGTTCAGC TCTCATGAAG ATAACCAAAC AACTAAATAG TGGTATTACA
    CCTCCGTTGC CCTCCAAGAC TGACAATTAT ATGTATGCAA AAATGCCAGG GGAAGGTTTG
    CAAGAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02362
    Clone ID Related Accession (Same CDS sequence) NM_001145053.1
    Accession Version NM_001145053.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303060.1, AK299608.1, DB076773.1, BC110609.1 and BX648689.1. Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR and coding region compared to variant 1. These differences cause translation initiation at a downstream in-frame AUG and a frame shift at the 3' coding region. The resulting isoform (b) is shorter at both termini and has a distinct C-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK299608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGATGAAA TCAAATCCCA AGAATTAAAC CTGGAGGGGC AGCGAAAAAT TTCACCTGGT 
    TCAATAAAGG ACTCTAAAAC TGAAGCCTCA GGTAATATTG CAATTAGAAA ATCTGCAAAA
    GTGATTTTTG CTTTAGATGA AACTGAACTG AAATCAAAGC CAGAGCACAC ATGGAAGAAA
    AACCTTTTTG AAAGAATGGA GGCAAGAGCC CAAGCAATGC AGCAGAAAAT AATAGATAAG
    GAAAATCTGA AGAAGGAACT AGAAAAAAAG GCTGAAAAAA AACTCCCTAG GGATAATTTG
    GCCAAAGAGT GGTTTAATAC TGACAGCATG ACACTGAATA ATACTGCATA TTTGCTTGAC
    AAACTTCTAC CCACCTTAGT TCCTGGAGTA GAAAACATGT TAACTCAAGT AGAAAAGAAG
    AAGGTTTTGA CAGAAGCTGA TACTCCAAGC AAGTTTGACC CAATTAATTA TTTGGGGGAA
    TATTTAATAA GAAACAATCC TAATTATATC AAAGACCCAG GAATGTCTGG TTACCAGAGG
    TTGATGAAAG AAGTCACAGA AGACCTGAAG ATATATGTTC CTGACACTAT CTGCAACAGA
    GTATCCAAGA TGAAGGAGAA TGTTAAACAG AATAGAAAAC AAAGAGAAAG CATAGACAAA
    ATCAAAGTCA AGGTGGCCAA CACACGGAAA CAGGCTCTGC AGGAGCAATT CGATGAATGG
    ATTCTAGACC CTAAAGGAAT GATTCCTAAG TCAGTGATTC AGAATGTTCT TCAAGAATTC
    TTTCAAAATC CAGATTTCAA GCTTGGATCA CACTGCAAAC AACTGGATAT TACTGACTCA
    ACAGAACCAA GATTGAACAA AATGGAATTT ACAGAATACA TCTCTTCACA TATTAAAGAC
    TTGAAGAGTG AAATGTTTGA GGAACTTCTT AAGCACCTTT GCCACTCTGC AGATGAATTT
    CGGGAGGTCA TAAAAGCTGA CATGCGGAGG CAGATGTTCG CTGAACTCTT CCTACATTGT
    GACCACGGGA AGGTAGGGTT TTTGGATCGG CAGAGGACAT TGGCCCTGCT GGAATTGTTC
    TATGACCATA GTTCACAAAT GCTTAGGAGT TTACTTCGAA ACCCACGACA ATGGCCATTC
    ATTGAATTTG AAGAGATAAA CTTGACTGAG TTGTGGGGAG ACATGGATAA TCAGAAACAC
    ATTTATGAAG GTTTTGACAA AGTGCTCTTG GAGATGAATA CATTACTTTC TGCAAATCAT
    GCTAGCAAAA CCCAAAGTAA ATTATTAGAA AGTCCAGATC AACCTAAACT TAACGAACAG
    AGAACATCAA CACCATCACC AAACCCGCCA GAACAGCAGA GAGGAGTAAC TGCAGAACAA
    GGACCACAAA GAATTTCAAT TGAAGAACAA CAACAAGGCA AAAAGCCAAC TGCAGAGCAA
    GAACTGTACA TAGAATCAGT AATAGAACCA GGAACACACA CAGAGTCAAC TCTAGAACAA
    GGGTCAAGTA GAAGGTTACT GACAGAACAA GAAACACACA GAGAGTCAAC TACAGAACAA
    GGACAGCACA AAGGGTCAAT AGAAGGACAA GGACCACGCA GAGTGTCAGT TTCAGAACAA
    GGATCAAGCA GAGAGTCAGT TGCAGAACAA GGGTCACGCA GAGAGTCTAT TGCAGAACAA
    GATCGACACA AAGGGTCAGT AGCAGAACAA GGATCACGCA GAATGTCAGC TGCAGAACAG
    GGATCACTCA GAGAGTCAGT AATAGAAGAA CCATACCAAA AATCAGAACA AGGACCTTAT
    GGAGAGATAA TTTCAGAAGA GCAAGAAGAC ATAGGCTCAA CTTCACAATC AAGAAAAGAT
    AGTATCTTAA AAAGTACAAA ATATGGGGAA CCTATAACCT CTGAGTACAT TGAAGTCCCT
    CTACAGGAAA AGAGGTCTTG GGAACAAACA TATGAAGAGG AAATATTCCT GAGTTCTGAA
    CTGCAAGAGG AAGTTCCAAC CTTAAGCAGA AAAGATCACT TTCCAGAAAC TACAAAAAAG
    GAAGTTCAGA AAGACAAGCC CTGTGAACCC AAGTCCCAAA AAATAGAAGG AAAGTCATGG
    TCAGGTGAAT TTTTTACTTG TAACTGGAAA ATGAAGTATG TCACATTTGA AGATGAGGAA
    CAGGCAAACT TAATCTATGG TAACTCCAGG TTCACAGATT TACACTCAAT TATCAGAAAT
    ATTCAGTCTT GCAAGGAGGT AAAAGGCAGA ACTGCCTTCA ATGGAGTTTC ATTCAATCTG
    CTCCAGTTTG TGCAACTCCT GGAGACATTT GTTGGTGAAG ACGCTCCCTT GAGTGTCAGC
    GAGACTCTCA CCTCCTTTTT TAAGGAGGGC TATGTTGAAA CAGAACAAGA GAAAATGAAT
    GCCTTAGAAC AGGTCTCACT CTGTCGCCAG GCTGGAGTGC AGTGGCGCAA CTGTAGCTCA
    CTGCAGCCTC CAACTCCCGG ACTCAAGCGA TCATCCCATC ACAGACTCCT GAGCAACTGG
    GACTACAGGG AAAGAAGGAA GTTTTTTCCT CTTGAGACCA AATTTCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138525.1
    CDS240..2810
    Translation

    Target ORF information:

    RefSeq Version NM_001145053.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145053.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGATGAAA TCAAATCCCA AGAATTAAAC CTGGAGGGGC AGCGAAAAAT TTCACCTGGT 
    TCAATAAAGG ACTCTAAAAC TGAAGCCTCA GGTAATATTG CAATTAGAAA ATCTGCAAAA
    GTGATTTTTG CTTTAGATGA AACTGAACTG AAATCAAAGC CAGAGCACAC ATGGAAGAAA
    AACCTTTTTG AAAGAATGGA GGCAAGAGCC CAAGCAATGC AGCAGAAAAT AATAGATAAG
    GAAAATCTGA AGAAGGAACT AGAAAAAAAG GCTGAAAAAA AACTCCCTAG GGATAATTTG
    GCCAAAGAGT GGTTTAATAC TGACAGCATG ACACTGAATA ATACTGCATA TTTGCTTGAC
    AAACTTCTAC CCACCTTAGT TCCTGGAGTA GAAAACATGT TAACTCAAGT AGAAAAGAAG
    AAGGTTTTGA CAGAAGCTGA TACTCCAAGC AAGTTTGACC CAATTAATTA TTTGGGGGAA
    TATTTAATAA GAAACAATCC TAATTATATC AAAGACCCAG GAATGTCTGG TTACCAGAGG
    TTGATGAAAG AAGTCACAGA AGACCTGAAG ATATATGTTC CTGACACTAT CTGCAACAGA
    GTATCCAAGA TGAAGGAGAA TGTTAAACAG AATAGAAAAC AAAGAGAAAG CATAGACAAA
    ATCAAAGTCA AGGTGGCCAA CACACGGAAA CAGGCTCTGC AGGAGCAATT CGATGAATGG
    ATTCTAGACC CTAAAGGAAT GATTCCTAAG TCAGTGATTC AGAATGTTCT TCAAGAATTC
    TTTCAAAATC CAGATTTCAA GCTTGGATCA CACTGCAAAC AACTGGATAT TACTGACTCA
    ACAGAACCAA GATTGAACAA AATGGAATTT ACAGAATACA TCTCTTCACA TATTAAAGAC
    TTGAAGAGTG AAATGTTTGA GGAACTTCTT AAGCACCTTT GCCACTCTGC AGATGAATTT
    CGGGAGGTCA TAAAAGCTGA CATGCGGAGG CAGATGTTCG CTGAACTCTT CCTACATTGT
    GACCACGGGA AGGTAGGGTT TTTGGATCGG CAGAGGACAT TGGCCCTGCT GGAATTGTTC
    TATGACCATA GTTCACAAAT GCTTAGGAGT TTACTTCGAA ACCCACGACA ATGGCCATTC
    ATTGAATTTG AAGAGATAAA CTTGACTGAG TTGTGGGGAG ACATGGATAA TCAGAAACAC
    ATTTATGAAG GTTTTGACAA AGTGCTCTTG GAGATGAATA CATTACTTTC TGCAAATCAT
    GCTAGCAAAA CCCAAAGTAA ATTATTAGAA AGTCCAGATC AACCTAAACT TAACGAACAG
    AGAACATCAA CACCATCACC AAACCCGCCA GAACAGCAGA GAGGAGTAAC TGCAGAACAA
    GGACCACAAA GAATTTCAAT TGAAGAACAA CAACAAGGCA AAAAGCCAAC TGCAGAGCAA
    GAACTGTACA TAGAATCAGT AATAGAACCA GGAACACACA CAGAGTCAAC TCTAGAACAA
    GGGTCAAGTA GAAGGTTACT GACAGAACAA GAAACACACA GAGAGTCAAC TACAGAACAA
    GGACAGCACA AAGGGTCAAT AGAAGGACAA GGACCACGCA GAGTGTCAGT TTCAGAACAA
    GGATCAAGCA GAGAGTCAGT TGCAGAACAA GGGTCACGCA GAGAGTCTAT TGCAGAACAA
    GATCGACACA AAGGGTCAGT AGCAGAACAA GGATCACGCA GAATGTCAGC TGCAGAACAG
    GGATCACTCA GAGAGTCAGT AATAGAAGAA CCATACCAAA AATCAGAACA AGGACCTTAT
    GGAGAGATAA TTTCAGAAGA GCAAGAAGAC ATAGGCTCAA CTTCACAATC AAGAAAAGAT
    AGTATCTTAA AAAGTACAAA ATATGGGGAA CCTATAACCT CTGAGTACAT TGAAGTCCCT
    CTACAGGAAA AGAGGTCTTG GGAACAAACA TATGAAGAGG AAATATTCCT GAGTTCTGAA
    CTGCAAGAGG AAGTTCCAAC CTTAAGCAGA AAAGATCACT TTCCAGAAAC TACAAAAAAG
    GAAGTTCAGA AAGACAAGCC CTGTGAACCC AAGTCCCAAA AAATAGAAGG AAAGTCATGG
    TCAGGTGAAT TTTTTACTTG TAACTGGAAA ATGAAGTATG TCACATTTGA AGATGAGGAA
    CAGGCAAACT TAATCTATGG TAACTCCAGG TTCACAGATT TACACTCAAT TATCAGAAAT
    ATTCAGTCTT GCAAGGAGGT AAAAGGCAGA ACTGCCTTCA ATGGAGTTTC ATTCAATCTG
    CTCCAGTTTG TGCAACTCCT GGAGACATTT GTTGGTGAAG ACGCTCCCTT GAGTGTCAGC
    GAGACTCTCA CCTCCTTTTT TAAGGAGGGC TATGTTGAAA CAGAACAAGA GAAAATGAAT
    GCCTTAGAAC AGGTCTCACT CTGTCGCCAG GCTGGAGTGC AGTGGCGCAA CTGTAGCTCA
    CTGCAGCCTC CAACTCCCGG ACTCAAGCGA TCATCCCATC ACAGACTCCT GAGCAACTGG
    GACTACAGGG AAAGAAGGAA GTTTTTTCCT CTTGAGACCA AATTTCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu79645
    Clone ID Related Accession (Same CDS sequence) XM_011524759.1
    Accession Version XM_011524759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGTTGCAG ATTCAGAATG TTCTTCAAGA ATTCTTTCAA
    AATCCAGATT TCAAGCTTGG ATCACACTGC AAACAACTGG ATATTACTGA CTCAACAGAA
    CCAAGATTGA ACAAAATGGA ATTTACAGAA TACATCTCTT CACATATTAA AGACTTGAAG
    AGTGAAATGT TTGAGGAACT TCTTAAGCAC CTTTGCCACT CTGCAGATGA ATTTCGGGAG
    GTCATAAAAG CTGACATGCG GAGGCAGATG TTCGCTGAAC TCTTCCTACA TTGTGACCAC
    GGGAAGGTAG GGTTTTTGGA TCGGCAGAGG ACATTGGCCC TGCTGGAATT GTTCTATGAC
    CATAGTTCAC AAATGCTTAG GAGTTTACTT CGAAACCCAC GACAATGGCC ATTCATTGAA
    TTTGAAGAGA TAAACTTGAC TGAGTTGTGG GGAGACATGG ATAATCAGAA ACACATTTAT
    GAAGGTTTTG ACAAAGTGCT CTTGGAGATG AATACATTAC TTTCTGCAAA TCATGCTAGC
    AAAACCCAAA GTAAATTATT AGAAAGTCCA GATCAACCTA AACTTAACGA ACAGAGAACA
    TCAACACCAT CACCAAACCC GCCAGAACAG CAGAGAGGAG TAACTGCAGA ACAAGGACCA
    CAAAGAATTT CAATTGAAGA ACAACAACAA GGCAAAAAGC CAACTGCAGA GCAAGAACTG
    TACATAGAAT CAGTAATAGA ACCAGGAACA CACACAGAGT CAACTCTAGA ACAAGGGTCA
    AGTAGAAGGT TACTGACAGA ACAAGAAACA CACAGAGAGT CAACTACAGA ACAAGGACAG
    CACAAAGGGT CAATAGAAGG ACAAGGACCA CGCAGAGTGT CAGTTTCAGA ACAAGGATCA
    AGCAGAGAGT CAGTTGCAGA ACAAGGGTCA CGCAGAGAGT CTATTGCAGA ACAAGATCGA
    CACAAAGGGT CAGTAGCAGA ACAAGGATCA CGCAGAATGT CAGCTGCAGA ACAGGGATCA
    CTCAGAGAGT CAGTAATAGA AGAACCATAC CAAAAATCAG AACAAGGACC TTATGGAGAG
    ATAATTTCAG AAGAGCAAGA AGACATAGGC TCAACTTCAC AATCAAGAAA AGATAGTATC
    TTAAAAAGTA CAAAATATGG GGAACCTATA ACCTCTGAGT ACATTGAAGT CCCTCTACAG
    GAAAAGAGGT CTTGGGAACA AACATATGAA GAGGAAATAT TCCTGAGTTC TGAACTGCAA
    GAGGAAGTTC CAACCTTAAG CAGAAAAGAT CACTTTCCAG AAACTACAAA AAAGGAAGTT
    CAGAAAGACA AGCCCTGTGA ACCCAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAATTTTTTA CTTGTAACTG GAAAATGAAG TATGTCACAT TTGAAGATGA GGAACAGGCA
    AACTTAATCT ATGGTAACTC CAGGTTCACA GATTTACACT CAATTATCAG AAATATTCAG
    TCTTGCAAGG AGGTAAAAGG CAGAACTGCC TTCAATGGAG TTTCATTCAA TCTGCTCCAG
    TTTGTGCAAC TCCTGGAGAC ATTTGTTGGT GAAGACGCTC CCTTGAGTGT CAGCGAGACT
    CTCACCTCCT TTTTTAAGGA GGGCTATGTT GAAACAGAAC AAGAGAAAAT GAATGCCTTA
    GAACAGTTTA GCCAAAATGC TTTCCAAGTC CGACAGAGGC TTCTCCTAGA AGCCATCTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC CTGGATCTGA AGGAAGTTGA TGAACTCTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCTATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACACCCTGG TCACGAAGTG AGATTGTCTT CAAAACAATT TCAGAATTAC
    ATAGAATTGG TTGTGTCTGA ACTCAGGGGC AATGAAGACC AAGTTCTGGA AAGTGTTGTG
    GAATTTCTGA TGAATGCTTT AGAGAGGAGC CACATTGAGA GTCTGAGGAA TTCTGCCAGG
    CGGAAATGGC TGCACCAAAT CCAATGTGCT GCAGAGACAA GTGGGGTGTC CCTAGAGCCG
    GTGTATAGTG AGACCTTTAA GGCCCTCATG CAGGATGCTG AAGCCCATGG AAATAAAAAG
    ATCAGTGCTC ACATTTCCCT CTTAGAAGAA AACCTACTAC TGCCTGAGAA AGGGAATGTT
    CTATTGAGGA ATGTGGCTTG TACCTTAGAT GATGCTCAAT TTGTACTGAA CAGAGTGCTC
    TACAGGGACA TGAAAGGAAT CAGCTTTACA GTAGTGGATG AAGGGAAGCC AATCCATGTT
    CCCCAAGTTC AGTACCATGG GAACATCTTC TTCTGGAACC AGTCCCGTAA TAAGCATGAT
    TATAATGGTT CATTCCTGGC TCTGCCTCTT CAAGATGCAT ATATGAGGAT CTTTGGGGTC
    TTGGCTGTTG ATACCCTTAG AGATCCCCAC GAAATAAACA TCTTTCTACC TCATGAGATC
    AGATTCTATC AGGGTGTTGC TAATGTCTTT AGCACTGCCT ATCACTACGT CCACAGCCGG
    GAGCACATTC TGCATATTGT GATCACTGGC ATAGGCTGGC TTTATGACGT CACATCCAGC
    ATCACCTCCA TCACTACGTA CTTTGTAGAG CCTAGCCCAG CCCAGGATTC AGACTATGTT
    TTACGCAACA TGATGGTTAC AGGGCAGCTG GGTCTAACAG AAATCCACAA AAATCCTCCT
    ACCATCCACA GGAAGTCATG CATCTTCAGA GATTTCCTCT TTAAATGTAC TGACAGTTCA
    GAAGTTGTTC TGGCTTCTGC CTGTGGAGAA ACGCATATAG TAGTTCCACT TCGTGAGAGA
    ACAGGAGAGG CTCTGGGAGT CCTCGATTTT AACATCGGCC AAAATAGGAT GTTGTTGTGT
    CAAGAATATA AAGATCTACA GAAAATGATG AAAGTGGTCC AAGTGGCCTG CTATGAAATA
    CTTGGCGAGT TCTCTGGAGA GATAAAGAAA AAATATATCT TAGAGATTGA AAATGTCAGG
    GAAGTCCAGC GGGCAGGAAT TCTCTTCTTC CGAATCATGC TGCTCGAGCT ACAGGAAAGC
    ATCCAACTAC TCAATTCCAT GGAATTTGTG TCACTGTTGC TCTATGACCA TACTCTTGTA
    ACAGAGCCAA ATTCTCCTCA AGACAGCAAA TCTATGGAGT TGGAAGCCAA CGTGAAACTA
    GTGCGTGACA TCCTGAAGGC GGTTATCTTG TTCTTTCATC CAGAGTTGGA ATTTTCAAGT
    GACTTTGGAA GTTGGGATAA GTGTAAATTT TATGTTAACA AATATTTAGT CAACAATATT
    TGTGCCTTTG ATCCAACTGC CAAGCATGTG GAAGTTAATG TACAGCTTAT TGATGAATAT
    ATCAGAGATC ATTCCCGAAC TGAAGTATGG AAATTTGGTA ATGTTGTCAT TGAACATCTA
    TACCACTGGA TACACATCTG TTCAGCTCTC ATGAAGATAA CCAAACAACT AAATAGTGGT
    ATTACACCTC CGTTGCCCTC CAAGACTGAC AATTATATGT ATGCAAAAAT GCCAGGGGAA
    GGTTTGCAAG AGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011523061.1
    CDS193..4710
    Translation

    Target ORF information:

    RefSeq Version XM_011524759.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGTTGCAG ATTCAGAATG TTCTTCAAGA ATTCTTTCAA
    AATCCAGATT TCAAGCTTGG ATCACACTGC AAACAACTGG ATATTACTGA CTCAACAGAA
    CCAAGATTGA ACAAAATGGA ATTTACAGAA TACATCTCTT CACATATTAA AGACTTGAAG
    AGTGAAATGT TTGAGGAACT TCTTAAGCAC CTTTGCCACT CTGCAGATGA ATTTCGGGAG
    GTCATAAAAG CTGACATGCG GAGGCAGATG TTCGCTGAAC TCTTCCTACA TTGTGACCAC
    GGGAAGGTAG GGTTTTTGGA TCGGCAGAGG ACATTGGCCC TGCTGGAATT GTTCTATGAC
    CATAGTTCAC AAATGCTTAG GAGTTTACTT CGAAACCCAC GACAATGGCC ATTCATTGAA
    TTTGAAGAGA TAAACTTGAC TGAGTTGTGG GGAGACATGG ATAATCAGAA ACACATTTAT
    GAAGGTTTTG ACAAAGTGCT CTTGGAGATG AATACATTAC TTTCTGCAAA TCATGCTAGC
    AAAACCCAAA GTAAATTATT AGAAAGTCCA GATCAACCTA AACTTAACGA ACAGAGAACA
    TCAACACCAT CACCAAACCC GCCAGAACAG CAGAGAGGAG TAACTGCAGA ACAAGGACCA
    CAAAGAATTT CAATTGAAGA ACAACAACAA GGCAAAAAGC CAACTGCAGA GCAAGAACTG
    TACATAGAAT CAGTAATAGA ACCAGGAACA CACACAGAGT CAACTCTAGA ACAAGGGTCA
    AGTAGAAGGT TACTGACAGA ACAAGAAACA CACAGAGAGT CAACTACAGA ACAAGGACAG
    CACAAAGGGT CAATAGAAGG ACAAGGACCA CGCAGAGTGT CAGTTTCAGA ACAAGGATCA
    AGCAGAGAGT CAGTTGCAGA ACAAGGGTCA CGCAGAGAGT CTATTGCAGA ACAAGATCGA
    CACAAAGGGT CAGTAGCAGA ACAAGGATCA CGCAGAATGT CAGCTGCAGA ACAGGGATCA
    CTCAGAGAGT CAGTAATAGA AGAACCATAC CAAAAATCAG AACAAGGACC TTATGGAGAG
    ATAATTTCAG AAGAGCAAGA AGACATAGGC TCAACTTCAC AATCAAGAAA AGATAGTATC
    TTAAAAAGTA CAAAATATGG GGAACCTATA ACCTCTGAGT ACATTGAAGT CCCTCTACAG
    GAAAAGAGGT CTTGGGAACA AACATATGAA GAGGAAATAT TCCTGAGTTC TGAACTGCAA
    GAGGAAGTTC CAACCTTAAG CAGAAAAGAT CACTTTCCAG AAACTACAAA AAAGGAAGTT
    CAGAAAGACA AGCCCTGTGA ACCCAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAATTTTTTA CTTGTAACTG GAAAATGAAG TATGTCACAT TTGAAGATGA GGAACAGGCA
    AACTTAATCT ATGGTAACTC CAGGTTCACA GATTTACACT CAATTATCAG AAATATTCAG
    TCTTGCAAGG AGGTAAAAGG CAGAACTGCC TTCAATGGAG TTTCATTCAA TCTGCTCCAG
    TTTGTGCAAC TCCTGGAGAC ATTTGTTGGT GAAGACGCTC CCTTGAGTGT CAGCGAGACT
    CTCACCTCCT TTTTTAAGGA GGGCTATGTT GAAACAGAAC AAGAGAAAAT GAATGCCTTA
    GAACAGTTTA GCCAAAATGC TTTCCAAGTC CGACAGAGGC TTCTCCTAGA AGCCATCTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC CTGGATCTGA AGGAAGTTGA TGAACTCTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCTATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACACCCTGG TCACGAAGTG AGATTGTCTT CAAAACAATT TCAGAATTAC
    ATAGAATTGG TTGTGTCTGA ACTCAGGGGC AATGAAGACC AAGTTCTGGA AAGTGTTGTG
    GAATTTCTGA TGAATGCTTT AGAGAGGAGC CACATTGAGA GTCTGAGGAA TTCTGCCAGG
    CGGAAATGGC TGCACCAAAT CCAATGTGCT GCAGAGACAA GTGGGGTGTC CCTAGAGCCG
    GTGTATAGTG AGACCTTTAA GGCCCTCATG CAGGATGCTG AAGCCCATGG AAATAAAAAG
    ATCAGTGCTC ACATTTCCCT CTTAGAAGAA AACCTACTAC TGCCTGAGAA AGGGAATGTT
    CTATTGAGGA ATGTGGCTTG TACCTTAGAT GATGCTCAAT TTGTACTGAA CAGAGTGCTC
    TACAGGGACA TGAAAGGAAT CAGCTTTACA GTAGTGGATG AAGGGAAGCC AATCCATGTT
    CCCCAAGTTC AGTACCATGG GAACATCTTC TTCTGGAACC AGTCCCGTAA TAAGCATGAT
    TATAATGGTT CATTCCTGGC TCTGCCTCTT CAAGATGCAT ATATGAGGAT CTTTGGGGTC
    TTGGCTGTTG ATACCCTTAG AGATCCCCAC GAAATAAACA TCTTTCTACC TCATGAGATC
    AGATTCTATC AGGGTGTTGC TAATGTCTTT AGCACTGCCT ATCACTACGT CCACAGCCGG
    GAGCACATTC TGCATATTGT GATCACTGGC ATAGGCTGGC TTTATGACGT CACATCCAGC
    ATCACCTCCA TCACTACGTA CTTTGTAGAG CCTAGCCCAG CCCAGGATTC AGACTATGTT
    TTACGCAACA TGATGGTTAC AGGGCAGCTG GGTCTAACAG AAATCCACAA AAATCCTCCT
    ACCATCCACA GGAAGTCATG CATCTTCAGA GATTTCCTCT TTAAATGTAC TGACAGTTCA
    GAAGTTGTTC TGGCTTCTGC CTGTGGAGAA ACGCATATAG TAGTTCCACT TCGTGAGAGA
    ACAGGAGAGG CTCTGGGAGT CCTCGATTTT AACATCGGCC AAAATAGGAT GTTGTTGTGT
    CAAGAATATA AAGATCTACA GAAAATGATG AAAGTGGTCC AAGTGGCCTG CTATGAAATA
    CTTGGCGAGT TCTCTGGAGA GATAAAGAAA AAATATATCT TAGAGATTGA AAATGTCAGG
    GAAGTCCAGC GGGCAGGAAT TCTCTTCTTC CGAATCATGC TGCTCGAGCT ACAGGAAAGC
    ATCCAACTAC TCAATTCCAT GGAATTTGTG TCACTGTTGC TCTATGACCA TACTCTTGTA
    ACAGAGCCAA ATTCTCCTCA AGACAGCAAA TCTATGGAGT TGGAAGCCAA CGTGAAACTA
    GTGCGTGACA TCCTGAAGGC GGTTATCTTG TTCTTTCATC CAGAGTTGGA ATTTTCAAGT
    GACTTTGGAA GTTGGGATAA GTGTAAATTT TATGTTAACA AATATTTAGT CAACAATATT
    TGTGCCTTTG ATCCAACTGC CAAGCATGTG GAAGTTAATG TACAGCTTAT TGATGAATAT
    ATCAGAGATC ATTCCCGAAC TGAAGTATGG AAATTTGGTA ATGTTGTCAT TGAACATCTA
    TACCACTGGA TACACATCTG TTCAGCTCTC ATGAAGATAA CCAAACAACT AAATAGTGGT
    ATTACACCTC CGTTGCCCTC CAAGACTGAC AATTATATGT ATGCAAAAAT GCCAGGGGAA
    GGTTTGCAAG AGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu79647
    Clone ID Related Accession (Same CDS sequence) XM_011524763.2
    Accession Version XM_011524763.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524763.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGTTGCAG ATTCAGAATG TTCTTCAAGA ATTCTTTCAA
    AATCCAGATT TCAAGCTTGG ATCACACTGC AAACAACTGG ATATTACTGA CTCAACAGAA
    CCAAGATTGA ACAAAATGGA ATTTACAGAA TACATCTCTT CACATATTAA AGACTTGAAG
    AGTGAAATGT TTGAGGAACT TCTTAAGCAC CTTTGCCACT CTGCAGATGA ATTTCGGGAG
    GTCATAAAAG CTGACATGCG GAGGCAGATG TTCGCTGAAC TCTTCCTACA TTGTGACCAC
    GGGAAGGTAG GGTTTTTGGA TCGGCAGAGG ACATTGGCCC TGCTGGAATT GTTCTATGAC
    CATAGTTCAC AAATGCTTAG GAGTTTACTT CGAAACCCAC GACAATGGCC ATTCATTGAA
    TTTGAAGAGA TAAACTTGAC TGAGTTGTGG GGAGACATGG ATAATCAGAA ACACATTTAT
    GAAGGTTTTG ACAAAGTGCT CTTGGAGATG AATACATTAC TTTCTGCAAA TCATGCTAGC
    AAAACCCAAA GTAAATTATT AGAAAGTCCA GATCAACCTA AACTTAACGA ACAGAGAACA
    TCAACACCAT CACCAAACCC GCCAGAACAG CAGAGAGGAG TAACTGCAGA ACAAGGACCA
    CAAAGAATTT CAATTGAAGA ACAACAACAA GGCAAAAAGC CAACTGCAGA GCAAGAACTG
    TACATAGAAT CAGTAATAGA ACCAGGAACA CACACAGAGT CAACTCTAGA ACAAGGGTCA
    AGTAGAAGGT TACTGACAGA ACAAGAAACA CACAGAGAGT CAACTACAGA ACAAGGACAG
    CACAAAGGGT CAATAGAAGG ACAAGGACCA CGCAGAGTGT CAGTTTCAGA ACAAGGATCA
    AGCAGAGAGT CAGTTGCAGA ACAAGGGTCA CGCAGAGAGT CTATTGCAGA ACAAGATCGA
    CACAAAGGGT CAGTAGCAGA ACAAGGATCA CGCAGAATGT CAGCTGCAGA ACAGGGATCA
    CTCAGAGAGT CAGTAATAGA AGAACCATAC CAAAAATCAG AACAAGGACC TTATGGAGAG
    ATAATTTCAG AAGAGCAAGA AGACATAGGC TCAACTTCAC AATCAAGAAA AGATAGTATC
    TTAAAAAGTA CAAAATATGG GGAACCTATA ACCTCTGAGT ACATTGAAGT CCCTCTACAG
    GAAAAGAGGT CTTGGGAACA AACATATGAA GAGGAAATAT TCCTGAGTTC TGAACTGCAA
    GAGGAAGTTC CAACCTTAAG CAGAAAAGAT CACTTTCCAG AAACTACAAA AAAGGAAGTT
    CAGAAAGACA AGCCCTGTGA ACCCAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAATTTTTTA CTTGTAACTG GAAAATGAAG TATGTCACAT TTGAAGATGA GGAACAGGCA
    AACTTAATCT ATGGTAACTC CAGGTTCACA GATTTACACT CAATTATCAG AAATATTCAG
    TCTTGCAAGG AGGTAAAAGG CAGAACTGCC TTCAATGGAG TTTCATTCAA TCTGCTCCAG
    TTTGTGCAAC TCCTGGAGAC ATTTGTTGGT GAAGACGCTC CCTTGAGTGT CAGCGAGACT
    CTCACCTCCT TTTTTAAGGA GGGCTATGTT GAAACAGAAC AAGAGAAAAT GAATGCCTTA
    GAACAGTTTA GCCAAAATGC TTTCCAAGTC CGACAGAGGC TTCTCCTAGA AGCCATCTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC CTGGATCTGA AGGAAGTTGA TGAACTCTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCTATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACACCCTGG TCACGAAGTG AGATTGTCTT CAAAACAATT TCAGAATTAC
    ATAGAATTGG TTGTGTCTGA ACTCAGGGGC AATGAAGACC AAGTTCTGGA AAGTGTTGTG
    GAATTTCTGA TGAATGCTTT AGAGAGGAGC CACATTGAGA GTCTGAGGAA TTCTGCCAGG
    CGGAAATGGC TGCACCAAAT CCAATGTGCT GCAGAGACAA GTGGGGTGTC CCTAGAGCCG
    GTGTATAGTG AGACCTTTAA GGCCCTCATG CAGGATGCTG AAGCCCATGG AAATAAAAAG
    ATCAGTGCTC ACATTTCCCT CTTAGAAGAA AACCTACTAC TGCCTGAGAA AGGGAATGTT
    CTATTGAGGA ATGTGGCTTG TACCTTAGAT GATGCTCAAT TTGTACTGAA CAGAGTGCTC
    TACAGGGACA TGAAAGGAAT CAGCTTTACA GTAGTGGATG AAGGGAAGCC AATCCATGTT
    CCCCAAGTTC AGTACCATGG GAACATCTTC TTCTGGAACC AGTCCCGTAA TAAGCATGAT
    TATAATGGTT CATTCCTGGC TCTGCCTCTT CAAGATGCAT ATATGAGGAT CTTTGGGGTC
    TTGGCTGTTG ATACCCTTAG AGATCCCCAC GAAATAAACA TCTTTCTACC TCATGAGATC
    AGATTCTATC AGGGTGTTGC TAATGTCTTT AGCACTGCCT ATCACTACGT CCACAGCCGG
    GAGCACATTC TGCATATTGT GATCACTGGC ATAGGCTGGC TTTATGACGT CACATCCAGC
    ATCACCTCCA TCACTACGTA CTTTGTAGAG CCTAGCCCAG CCCAGGATTC AGACTATGTT
    TTACGCAACA TGATGGTTAC AGGGCAGCTG GGTCTAACAG AAATCCACAA AAATCCTCCT
    ACCATCCACA GGAAGTCATG CATCTTCAGA GATTTCCTCT TTAAATGTAC TGACAGTTCA
    GAAGTTGTTC TGGCTTCTGC CTGTGGAGAA ACGCATATAG TAGTTCCACT TCGTGAGAGA
    ACAGGAGAGG CTCTGGGAGT CCTCGATTTT AACATCGGCC AAAATAGGAT GTTGTTGTGT
    CAAGAATATA AAGATCTACA GAAAATGATG AAAGTGGTCC AAGTGGCCTG CTATGAAATA
    CTTGGCGAGT TCTCTGGAGA GATAAAGAAA AAATATATCT TAGAGATTGA AAATGTCAGG
    GAAGTCCAGC GGGCAGGAAT TCTCTTCTTC CGAATCATGC TGCTCGAGCT ACAGGAAAGC
    ATCCAACTAC TCAATTCCAT GGAATTTGTG TCACTGTTGC TCTATGACCA TACTCTTGTA
    ACAGAGCCAA ATTCTCCTCA AGACAGCAAA TCTATGGAGT TGGAAGCCAA CGTGAAACTA
    GTGCGTGACA TCCTGAAGGC GGTTATCTTG TTCTTTCATC CAGAGTTGGA ATTTTCAAGT
    GACTTTGGAA GTTGGGATAA GTGTAAATTT TATGTTAACA AATATTTAGT CAACAATATT
    TGTGCCTTTG ATCCAACTGC CAAGCATGTG GAAGTTAATG TACAGCTTAT TGATGAATAT
    ATCAGAGGCT CTAGACCAAC GCAGAAGAGT TTCTGGATTA ACGGGGAACT ATCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011523065.1
    CDS193..4569
    Translation

    Target ORF information:

    RefSeq Version XM_011524763.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524763.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGTTGCAG ATTCAGAATG TTCTTCAAGA ATTCTTTCAA
    AATCCAGATT TCAAGCTTGG ATCACACTGC AAACAACTGG ATATTACTGA CTCAACAGAA
    CCAAGATTGA ACAAAATGGA ATTTACAGAA TACATCTCTT CACATATTAA AGACTTGAAG
    AGTGAAATGT TTGAGGAACT TCTTAAGCAC CTTTGCCACT CTGCAGATGA ATTTCGGGAG
    GTCATAAAAG CTGACATGCG GAGGCAGATG TTCGCTGAAC TCTTCCTACA TTGTGACCAC
    GGGAAGGTAG GGTTTTTGGA TCGGCAGAGG ACATTGGCCC TGCTGGAATT GTTCTATGAC
    CATAGTTCAC AAATGCTTAG GAGTTTACTT CGAAACCCAC GACAATGGCC ATTCATTGAA
    TTTGAAGAGA TAAACTTGAC TGAGTTGTGG GGAGACATGG ATAATCAGAA ACACATTTAT
    GAAGGTTTTG ACAAAGTGCT CTTGGAGATG AATACATTAC TTTCTGCAAA TCATGCTAGC
    AAAACCCAAA GTAAATTATT AGAAAGTCCA GATCAACCTA AACTTAACGA ACAGAGAACA
    TCAACACCAT CACCAAACCC GCCAGAACAG CAGAGAGGAG TAACTGCAGA ACAAGGACCA
    CAAAGAATTT CAATTGAAGA ACAACAACAA GGCAAAAAGC CAACTGCAGA GCAAGAACTG
    TACATAGAAT CAGTAATAGA ACCAGGAACA CACACAGAGT CAACTCTAGA ACAAGGGTCA
    AGTAGAAGGT TACTGACAGA ACAAGAAACA CACAGAGAGT CAACTACAGA ACAAGGACAG
    CACAAAGGGT CAATAGAAGG ACAAGGACCA CGCAGAGTGT CAGTTTCAGA ACAAGGATCA
    AGCAGAGAGT CAGTTGCAGA ACAAGGGTCA CGCAGAGAGT CTATTGCAGA ACAAGATCGA
    CACAAAGGGT CAGTAGCAGA ACAAGGATCA CGCAGAATGT CAGCTGCAGA ACAGGGATCA
    CTCAGAGAGT CAGTAATAGA AGAACCATAC CAAAAATCAG AACAAGGACC TTATGGAGAG
    ATAATTTCAG AAGAGCAAGA AGACATAGGC TCAACTTCAC AATCAAGAAA AGATAGTATC
    TTAAAAAGTA CAAAATATGG GGAACCTATA ACCTCTGAGT ACATTGAAGT CCCTCTACAG
    GAAAAGAGGT CTTGGGAACA AACATATGAA GAGGAAATAT TCCTGAGTTC TGAACTGCAA
    GAGGAAGTTC CAACCTTAAG CAGAAAAGAT CACTTTCCAG AAACTACAAA AAAGGAAGTT
    CAGAAAGACA AGCCCTGTGA ACCCAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAATTTTTTA CTTGTAACTG GAAAATGAAG TATGTCACAT TTGAAGATGA GGAACAGGCA
    AACTTAATCT ATGGTAACTC CAGGTTCACA GATTTACACT CAATTATCAG AAATATTCAG
    TCTTGCAAGG AGGTAAAAGG CAGAACTGCC TTCAATGGAG TTTCATTCAA TCTGCTCCAG
    TTTGTGCAAC TCCTGGAGAC ATTTGTTGGT GAAGACGCTC CCTTGAGTGT CAGCGAGACT
    CTCACCTCCT TTTTTAAGGA GGGCTATGTT GAAACAGAAC AAGAGAAAAT GAATGCCTTA
    GAACAGTTTA GCCAAAATGC TTTCCAAGTC CGACAGAGGC TTCTCCTAGA AGCCATCTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC CTGGATCTGA AGGAAGTTGA TGAACTCTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCTATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACACCCTGG TCACGAAGTG AGATTGTCTT CAAAACAATT TCAGAATTAC
    ATAGAATTGG TTGTGTCTGA ACTCAGGGGC AATGAAGACC AAGTTCTGGA AAGTGTTGTG
    GAATTTCTGA TGAATGCTTT AGAGAGGAGC CACATTGAGA GTCTGAGGAA TTCTGCCAGG
    CGGAAATGGC TGCACCAAAT CCAATGTGCT GCAGAGACAA GTGGGGTGTC CCTAGAGCCG
    GTGTATAGTG AGACCTTTAA GGCCCTCATG CAGGATGCTG AAGCCCATGG AAATAAAAAG
    ATCAGTGCTC ACATTTCCCT CTTAGAAGAA AACCTACTAC TGCCTGAGAA AGGGAATGTT
    CTATTGAGGA ATGTGGCTTG TACCTTAGAT GATGCTCAAT TTGTACTGAA CAGAGTGCTC
    TACAGGGACA TGAAAGGAAT CAGCTTTACA GTAGTGGATG AAGGGAAGCC AATCCATGTT
    CCCCAAGTTC AGTACCATGG GAACATCTTC TTCTGGAACC AGTCCCGTAA TAAGCATGAT
    TATAATGGTT CATTCCTGGC TCTGCCTCTT CAAGATGCAT ATATGAGGAT CTTTGGGGTC
    TTGGCTGTTG ATACCCTTAG AGATCCCCAC GAAATAAACA TCTTTCTACC TCATGAGATC
    AGATTCTATC AGGGTGTTGC TAATGTCTTT AGCACTGCCT ATCACTACGT CCACAGCCGG
    GAGCACATTC TGCATATTGT GATCACTGGC ATAGGCTGGC TTTATGACGT CACATCCAGC
    ATCACCTCCA TCACTACGTA CTTTGTAGAG CCTAGCCCAG CCCAGGATTC AGACTATGTT
    TTACGCAACA TGATGGTTAC AGGGCAGCTG GGTCTAACAG AAATCCACAA AAATCCTCCT
    ACCATCCACA GGAAGTCATG CATCTTCAGA GATTTCCTCT TTAAATGTAC TGACAGTTCA
    GAAGTTGTTC TGGCTTCTGC CTGTGGAGAA ACGCATATAG TAGTTCCACT TCGTGAGAGA
    ACAGGAGAGG CTCTGGGAGT CCTCGATTTT AACATCGGCC AAAATAGGAT GTTGTTGTGT
    CAAGAATATA AAGATCTACA GAAAATGATG AAAGTGGTCC AAGTGGCCTG CTATGAAATA
    CTTGGCGAGT TCTCTGGAGA GATAAAGAAA AAATATATCT TAGAGATTGA AAATGTCAGG
    GAAGTCCAGC GGGCAGGAAT TCTCTTCTTC CGAATCATGC TGCTCGAGCT ACAGGAAAGC
    ATCCAACTAC TCAATTCCAT GGAATTTGTG TCACTGTTGC TCTATGACCA TACTCTTGTA
    ACAGAGCCAA ATTCTCCTCA AGACAGCAAA TCTATGGAGT TGGAAGCCAA CGTGAAACTA
    GTGCGTGACA TCCTGAAGGC GGTTATCTTG TTCTTTCATC CAGAGTTGGA ATTTTCAAGT
    GACTTTGGAA GTTGGGATAA GTGTAAATTT TATGTTAACA AATATTTAGT CAACAATATT
    TGTGCCTTTG ATCCAACTGC CAAGCATGTG GAAGTTAATG TACAGCTTAT TGATGAATAT
    ATCAGAGGCT CTAGACCAAC GCAGAAGAGT TTCTGGATTA ACGGGGAACT ATCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu79649
    Clone ID Related Accession (Same CDS sequence) XM_011524765.1
    Accession Version XM_011524765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGTTGCAG ATTCAGAATG TTCTTCAAGA ATTCTTTCAA
    AATCCAGATT TCAAGCTTGG ATCACACTGC AAACAACTGG ATATTACTGA CTCAACAGAA
    CCAAGATTGA ACAAAATGGA ATTTACAGAA TACATCTCTT CACATATTAA AGACTTGAAG
    AGTGAAATGT TTGAGGAACT TCTTAAGCAC CTTTGCCACT CTGCAGATGA ATTTCGGGAG
    GTCATAAAAG CTGACATGCG GAGGCAGATG TTCGCTGAAC TCTTCCTACA TTGTGACCAC
    GGGAAGGTAG GGTTTTTGGA TCGGCAGAGG ACATTGGCCC TGCTGGAATT GTTCTATGAC
    CATAGTTCAC AAATGCTTAG GAGTTTACTT CGAAACCCAC GACAATGGCC ATTCATTGAA
    TTTGAAGAGA TAAACTTGAC TGAGTTGTGG GGAGACATGG ATAATCAGAA ACACATTTAT
    GAAGGTTTTG ACAAAGTGCT CTTGGAGATG AATACATTAC TTTCTGCAAA TCATGCTAGC
    AAAACCCAAA GTAAATTATT AGAAAGTCCA GATCAACCTA AACTTAACGA ACAGAGAACA
    TCAACACCAT CACCAAACCC GCCAGAACAG CAGAGAGGAG TAACTGCAGA ACAAGGACCA
    CAAAGAATTT CAATTGAAGA ACAACAACAA GGCAAAAAGC CAACTGCAGA GCAAGAACTG
    TACATAGAAT CAGTAATAGA ACCAGGAACA CACACAGAGT CAACTCTAGA ACAAGGGTCA
    AGTAGAAGGT TACTGACAGA ACAAGAAACA CACAGAGAGT CAACTACAGA ACAAGGACAG
    CACAAAGGGT CAATAGAAGG ACAAGGACCA CGCAGAGTGT CAGTTTCAGA ACAAGGATCA
    AGCAGAGAGT CAGTTGCAGA ACAAGGGTCA CGCAGAGAGT CTATTGCAGA ACAAGATCGA
    CACAAAGGGT CAGTAGCAGA ACAAGGATCA CGCAGAATGT CAGCTGCAGA ACAGGGATCA
    CTCAGAGAGT CAGTAATAGA AGAACCATAC CAAAAATCAG AACAAGGACC TTATGGAGAG
    ATAATTTCAG AAGAGCAAGA AGACATAGGC TCAACTTCAC AATCAAGAAA AGATAGTATC
    TTAAAAAGTA CAAAATATGG GGAACCTATA ACCTCTGAGT ACATTGAAGT CCCTCTACAG
    GAAAAGAGGT CTTGGGAACA AACATATGAA GAGGAAATAT TCCTGAGTTC TGAACTGCAA
    GAGGAAGTTC CAACCTTAAG CAGAAAAGAT CACTTTCCAG AAACTACAAA AAAGGAAGTT
    CAGAAAGACA AGCCCTGTGA ACCCAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAATTTTTTA CTTGTAACTG GAAAATGAAG TATGTCACAT TTGAAGATGA GGAACAGGCA
    AACTTAATCT ATGGTAACTC CAGGTTCACA GATTTACACT CAATTATCAG AAATATTCAG
    TCTTGCAAGG AGGTAAAAGG CAGAACTGCC TTCAATGGAG TTTCATTCAA TCTGCTCCAG
    TTTGTGCAAC TCCTGGAGAC ATTTGTTGGT GAAGACGCTC CCTTGAGTGT CAGCGAGACT
    CTCACCTCCT TTTTTAAGGA GGGCTATGTT GAAACAGAAC AAGAGAAAAT GAATGCCTTA
    GAACAGTTTA GCCAAAATGC TTTCCAAGTC CGACAGAGGC TTCTCCTAGA AGCCATCTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC CTGGATCTGA AGGAAGTTGA TGAACTCTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCTATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACACCCTGG TCACGAAGTG AGATTGTCTT CAAAACAATT TCAGAATTAC
    ATAGAATTGG TTGTGTCTGA ACTCAGGGGC AATGAAGACC AAGTTCTGGA AAGTGTTGTG
    GAATTTCTGA TGAATGCTTT AGAGAGGAGC CACATTGAGA GTCTGAGGAA TTCTGCCAGG
    CGGAAATGGC TGCACCAAAT CCAATGTGCT GCAGAGACAA GTGGGGTGTC CCTAGAGCCG
    GTGTATAGTG AGACCTTTAA GGCCCTCATG CAGGATGCTG AAGCCCATGG AAATAAAAAG
    ATCAGTGCTC ACATTTCCCT CTTAGAAGAA AACCTACTAC TGCCTGAGAA AGGGAATGTT
    CTATTGAGGA ATGTGGCTTG TACCTTAGAT GATGCTCAAT TTGTACTGAA CAGAGTGCTC
    TACAGGGACA TGAAAGGAAT CAGCTTTACA GTAGTGGATG AAGGGAAGCC AATCCATGTT
    CCCCAAGTTC AGTACCATGG GAACATCTTC TTCTGGAACC AGTCCCGTAA TAAGCATGAT
    TATAATGGTT CATTCCTGGC TCTGCCTCTT CAAGATGCAT ATATGAGGAT CTTTGGGGTC
    TTGGCTGTTG ATACCCTTAG AGATCCCCAC GAAATAAACA TCTTTCTACC TCATGAGATC
    AGATTCTATC AGGGTGTTGC TAATGTCTTT AGCACTGCCT ATCACTACGT CCACAGCCGG
    GAGCACATTC TGCATATTGT GATCACTGGC ATAGGCTGGC TTTATGACGT CACATCCAGC
    ATCACCTCCA TCACTACGTA CTTTGTAGAG CCTAGCCCAG CCCAGGATTC AGACTATGTT
    TTACGCAACA TGATGGTTAC AGGGCAGCTG GGTCTAACAG AAATCCACAA AAATCCTCCT
    ACCATCCACA GGAAGTCATG CATCTTCAGA TCATTCCCGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011523067.1
    CDS193..3897
    Translation

    Target ORF information:

    RefSeq Version XM_011524765.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGTTGCAG ATTCAGAATG TTCTTCAAGA ATTCTTTCAA
    AATCCAGATT TCAAGCTTGG ATCACACTGC AAACAACTGG ATATTACTGA CTCAACAGAA
    CCAAGATTGA ACAAAATGGA ATTTACAGAA TACATCTCTT CACATATTAA AGACTTGAAG
    AGTGAAATGT TTGAGGAACT TCTTAAGCAC CTTTGCCACT CTGCAGATGA ATTTCGGGAG
    GTCATAAAAG CTGACATGCG GAGGCAGATG TTCGCTGAAC TCTTCCTACA TTGTGACCAC
    GGGAAGGTAG GGTTTTTGGA TCGGCAGAGG ACATTGGCCC TGCTGGAATT GTTCTATGAC
    CATAGTTCAC AAATGCTTAG GAGTTTACTT CGAAACCCAC GACAATGGCC ATTCATTGAA
    TTTGAAGAGA TAAACTTGAC TGAGTTGTGG GGAGACATGG ATAATCAGAA ACACATTTAT
    GAAGGTTTTG ACAAAGTGCT CTTGGAGATG AATACATTAC TTTCTGCAAA TCATGCTAGC
    AAAACCCAAA GTAAATTATT AGAAAGTCCA GATCAACCTA AACTTAACGA ACAGAGAACA
    TCAACACCAT CACCAAACCC GCCAGAACAG CAGAGAGGAG TAACTGCAGA ACAAGGACCA
    CAAAGAATTT CAATTGAAGA ACAACAACAA GGCAAAAAGC CAACTGCAGA GCAAGAACTG
    TACATAGAAT CAGTAATAGA ACCAGGAACA CACACAGAGT CAACTCTAGA ACAAGGGTCA
    AGTAGAAGGT TACTGACAGA ACAAGAAACA CACAGAGAGT CAACTACAGA ACAAGGACAG
    CACAAAGGGT CAATAGAAGG ACAAGGACCA CGCAGAGTGT CAGTTTCAGA ACAAGGATCA
    AGCAGAGAGT CAGTTGCAGA ACAAGGGTCA CGCAGAGAGT CTATTGCAGA ACAAGATCGA
    CACAAAGGGT CAGTAGCAGA ACAAGGATCA CGCAGAATGT CAGCTGCAGA ACAGGGATCA
    CTCAGAGAGT CAGTAATAGA AGAACCATAC CAAAAATCAG AACAAGGACC TTATGGAGAG
    ATAATTTCAG AAGAGCAAGA AGACATAGGC TCAACTTCAC AATCAAGAAA AGATAGTATC
    TTAAAAAGTA CAAAATATGG GGAACCTATA ACCTCTGAGT ACATTGAAGT CCCTCTACAG
    GAAAAGAGGT CTTGGGAACA AACATATGAA GAGGAAATAT TCCTGAGTTC TGAACTGCAA
    GAGGAAGTTC CAACCTTAAG CAGAAAAGAT CACTTTCCAG AAACTACAAA AAAGGAAGTT
    CAGAAAGACA AGCCCTGTGA ACCCAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAATTTTTTA CTTGTAACTG GAAAATGAAG TATGTCACAT TTGAAGATGA GGAACAGGCA
    AACTTAATCT ATGGTAACTC CAGGTTCACA GATTTACACT CAATTATCAG AAATATTCAG
    TCTTGCAAGG AGGTAAAAGG CAGAACTGCC TTCAATGGAG TTTCATTCAA TCTGCTCCAG
    TTTGTGCAAC TCCTGGAGAC ATTTGTTGGT GAAGACGCTC CCTTGAGTGT CAGCGAGACT
    CTCACCTCCT TTTTTAAGGA GGGCTATGTT GAAACAGAAC AAGAGAAAAT GAATGCCTTA
    GAACAGTTTA GCCAAAATGC TTTCCAAGTC CGACAGAGGC TTCTCCTAGA AGCCATCTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC CTGGATCTGA AGGAAGTTGA TGAACTCTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCTATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACACCCTGG TCACGAAGTG AGATTGTCTT CAAAACAATT TCAGAATTAC
    ATAGAATTGG TTGTGTCTGA ACTCAGGGGC AATGAAGACC AAGTTCTGGA AAGTGTTGTG
    GAATTTCTGA TGAATGCTTT AGAGAGGAGC CACATTGAGA GTCTGAGGAA TTCTGCCAGG
    CGGAAATGGC TGCACCAAAT CCAATGTGCT GCAGAGACAA GTGGGGTGTC CCTAGAGCCG
    GTGTATAGTG AGACCTTTAA GGCCCTCATG CAGGATGCTG AAGCCCATGG AAATAAAAAG
    ATCAGTGCTC ACATTTCCCT CTTAGAAGAA AACCTACTAC TGCCTGAGAA AGGGAATGTT
    CTATTGAGGA ATGTGGCTTG TACCTTAGAT GATGCTCAAT TTGTACTGAA CAGAGTGCTC
    TACAGGGACA TGAAAGGAAT CAGCTTTACA GTAGTGGATG AAGGGAAGCC AATCCATGTT
    CCCCAAGTTC AGTACCATGG GAACATCTTC TTCTGGAACC AGTCCCGTAA TAAGCATGAT
    TATAATGGTT CATTCCTGGC TCTGCCTCTT CAAGATGCAT ATATGAGGAT CTTTGGGGTC
    TTGGCTGTTG ATACCCTTAG AGATCCCCAC GAAATAAACA TCTTTCTACC TCATGAGATC
    AGATTCTATC AGGGTGTTGC TAATGTCTTT AGCACTGCCT ATCACTACGT CCACAGCCGG
    GAGCACATTC TGCATATTGT GATCACTGGC ATAGGCTGGC TTTATGACGT CACATCCAGC
    ATCACCTCCA TCACTACGTA CTTTGTAGAG CCTAGCCCAG CCCAGGATTC AGACTATGTT
    TTACGCAACA TGATGGTTAC AGGGCAGCTG GGTCTAACAG AAATCCACAA AAATCCTCCT
    ACCATCCACA GGAAGTCATG CATCTTCAGA TCATTCCCGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu79650
    Clone ID Related Accession (Same CDS sequence) XM_011524766.2
    Accession Version XM_011524766.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAATTTA CAGAATACAT CTCTTCACAT ATTAAAGACT TGAAGAGTGA AATGTTTGAG 
    GAACTTCTTA AGCACCTTTG CCACTCTGCA GATGAATTTC GGGAGGTCAT AAAAGCTGAC
    ATGCGGAGGC AGATGTTCGC TGAACTCTTC CTACATTGTG ACCACGGGAA GGTAGGGTTT
    TTGGATCGGC AGAGGACATT GGCCCTGCTG GAATTGTTCT ATGACCATAG TTCACAAATG
    CTTAGGAGTT TACTTCGAAA CCCACGACAA TGGCCATTCA TTGAATTTGA AGAGATAAAC
    TTGACTGAGT TGTGGGGAGA CATGGATAAT CAGAAACACA TTTATGAAGG TTTTGACAAA
    GTGCTCTTGG AGATGAATAC ATTACTTTCT GCAAATCATG CTAGCAAAAC CCAAAGTAAA
    TTATTAGAAA GTCCAGATCA ACCTAAACTT AACGAACAGA GAACATCAAC ACCATCACCA
    AACCCGCCAG AACAGCAGAG AGGAGTAACT GCAGAACAAG GACCACAAAG AATTTCAATT
    GAAGAACAAC AACAAGGCAA AAAGCCAACT GCAGAGCAAG AACTGTACAT AGAATCAGTA
    ATAGAACCAG GAACACACAC AGAGTCAACT CTAGAACAAG GGTCAAGTAG AAGGTTACTG
    ACAGAACAAG AAACACACAG AGAGTCAACT ACAGAACAAG GACAGCACAA AGGGTCAATA
    GAAGGACAAG GACCACGCAG AGTGTCAGTT TCAGAACAAG GATCAAGCAG AGAGTCAGTT
    GCAGAACAAG GGTCACGCAG AGAGTCTATT GCAGAACAAG ATCGACACAA AGGGTCAGTA
    GCAGAACAAG GATCACGCAG AATGTCAGCT GCAGAACAGG GATCACTCAG AGAGTCAGTA
    ATAGAAGAAC CATACCAAAA ATCAGAACAA GGACCTTATG GAGAGATAAT TTCAGAAGAG
    CAAGAAGACA TAGGCTCAAC TTCACAATCA AGAAAAGATA GTATCTTAAA AAGTACAAAA
    TATGGGGAAC CTATAACCTC TGAGTACATT GAAGTCCCTC TACAGGAAAA GAGGTCTTGG
    GAACAAACAT ATGAAGAGGA AATATTCCTG AGTTCTGAAC TGCAAGAGGA AGTTCCAACC
    TTAAGCAGAA AAGATCACTT TCCAGAAACT ACAAAAAAGG AAGTTCAGAA AGACAAGCCC
    TGTGAACCCA AGTCCCAAAA AATAGAAGGA AAGTCATGGT CAGGTGAATT TTTTACTTGT
    AACTGGAAAA TGAAGTATGT CACATTTGAA GATGAGGAAC AGGCAAACTT AATCTATGGT
    AACTCCAGGT TCACAGATTT ACACTCAATT ATCAGAAATA TTCAGTCTTG CAAGGAGGTA
    AAAGGCAGAA CTGCCTTCAA TGGAGTTTCA TTCAATCTGC TCCAGTTTGT GCAACTCCTG
    GAGACATTTG TTGGTGAAGA CGCTCCCTTG AGTGTCAGCG AGACTCTCAC CTCCTTTTTT
    AAGGAGGGCT ATGTTGAAAC AGAACAAGAG AAAATGAATG CCTTAGAACA GTTTAGCCAA
    AATGCTTTCC AAGTCCGACA GAGGCTTCTC CTAGAAGCCA TCTTTCAGAA GTGGGACAGT
    GATGGCTCAG GCTTCCTGGA TCTGAAGGAA GTTGATGAAC TCTTGTACAC ATACAAGGAG
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT AAACTACACA TCCAATTTCC AAAGCCACAC
    CCTGGTCACG AAGTGAGATT GTCTTCAAAA CAATTTCAGA ATTACATAGA ATTGGTTGTG
    TCTGAACTCA GGGGCAATGA AGACCAAGTT CTGGAAAGTG TTGTGGAATT TCTGATGAAT
    GCTTTAGAGA GGAGCCACAT TGAGAGTCTG AGGAATTCTG CCAGGCGGAA ATGGCTGCAC
    CAAATCCAAT GTGCTGCAGA GACAAGTGGG GTGTCCCTAG AGCCGGTGTA TAGTGAGACC
    TTTAAGGCCC TCATGCAGGA TGCTGAAGCC CATGGAAATA AAAAGATCAG TGCTCACATT
    TCCCTCTTAG AAGAAAACCT ACTACTGCCT GAGAAAGGGA ATGTTCTATT GAGGAATGTG
    GCTTGTACCT TAGATGATGC TCAATTTGTA CTGAACAGAG TGCTCTACAG GGACATGAAA
    GGAATCAGCT TTACAGTAGT GGATGAAGGG AAGCCAATCC ATGTTCCCCA AGTTCAGTAC
    CATGGGAACA TCTTCTTCTG GAACCAGTCC CGTAATAAGC ATGATTATAA TGGTTCATTC
    CTGGCTCTGC CTCTTCAAGA TGCATATATG AGGATCTTTG GGGTCTTGGC TGTTGATACC
    CTTAGAGATC CCCACGAAAT AAACATCTTT CTACCTCATG AGATCAGATT CTATCAGGGT
    GTTGCTAATG TCTTTAGCAC TGCCTATCAC TACGTCCACA GCCGGGAGCA CATTCTGCAT
    ATTGTGATCA CTGGCATAGG CTGGCTTTAT GACGTCACAT CCAGCATCAC CTCCATCACT
    ACGTACTTTG TAGAGCCTAG CCCAGCCCAG GATTCAGACT ATGTTTTACG CAACATGATG
    GTTACAGGGC AGCTGGGTCT AACAGAAATC CACAAAAATC CTCCTACCAT CCACAGGAAG
    TCATGCATCT TCAGAGATTT CCTCTTTAAA TGTACTGACA GTTCAGAAGT TGTTCTGGCT
    TCTGCCTGTG GAGAAACGCA TATAGTAGTT CCACTTCGTG AGAGAACAGG AGAGGCTCTG
    GGAGTCCTCG ATTTTAACAT CGGCCAAAAT AGGATGTTGT TGTGTCAAGA ATATAAAGAT
    CTACAGAAAA TGATGAAAGT GGTCCAAGTG GCCTGCTATG AAATACTTGG CGAGTTCTCT
    GGAGAGATAA AGAAAAAATA TATCTTAGAG ATTGAAAATG TCAGGGAAGT CCAGCGGGCA
    GGAATTCTCT TCTTCCGAAT CATGCTGCTC GAGCTACAGG AAAGCATCCA ACTACTCAAT
    TCCATGGAAT TTGTGTCACT GTTGCTCTAT GACCATACTC TTGTAACAGA GCCAAATTCT
    CCTCAAGACA GCAAATCTAT GGAGTTGGAA GCCAACGTGA AACTAGTGCG TGACATCCTG
    AAGGCGGTTA TCTTGTTCTT TCATCCAGAG TTGGAATTTT CAAGTGACTT TGGAAGTTGG
    GATAAGTGTA AATTTTATGT TAACAAATAT TTAGTCAACA ATATTTGTGC CTTTGATCCA
    ACTGCCAAGC ATGTGGAAGT TAATGTACAG CTTATTGATG AATATATCAG AGATCATTCC
    CGAACTGAAG TATGGAAATT TGGTAATGTT GTCATTGAAC ATCTATACCA CTGGATACAC
    ATCTGTTCAG CTCTCATGAA GATAACCAAA CAACTAAATA GTGGTATTAC ACCTCCGTTG
    CCCTCCAAGA CTGACAATTA TATGTATGCA AAAATGCCAG GGGAAGGTTT GCAAGAGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011523068.1
    CDS168..3650
    Translation

    Target ORF information:

    RefSeq Version XM_011524766.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524766.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAATTTA CAGAATACAT CTCTTCACAT ATTAAAGACT TGAAGAGTGA AATGTTTGAG 
    GAACTTCTTA AGCACCTTTG CCACTCTGCA GATGAATTTC GGGAGGTCAT AAAAGCTGAC
    ATGCGGAGGC AGATGTTCGC TGAACTCTTC CTACATTGTG ACCACGGGAA GGTAGGGTTT
    TTGGATCGGC AGAGGACATT GGCCCTGCTG GAATTGTTCT ATGACCATAG TTCACAAATG
    CTTAGGAGTT TACTTCGAAA CCCACGACAA TGGCCATTCA TTGAATTTGA AGAGATAAAC
    TTGACTGAGT TGTGGGGAGA CATGGATAAT CAGAAACACA TTTATGAAGG TTTTGACAAA
    GTGCTCTTGG AGATGAATAC ATTACTTTCT GCAAATCATG CTAGCAAAAC CCAAAGTAAA
    TTATTAGAAA GTCCAGATCA ACCTAAACTT AACGAACAGA GAACATCAAC ACCATCACCA
    AACCCGCCAG AACAGCAGAG AGGAGTAACT GCAGAACAAG GACCACAAAG AATTTCAATT
    GAAGAACAAC AACAAGGCAA AAAGCCAACT GCAGAGCAAG AACTGTACAT AGAATCAGTA
    ATAGAACCAG GAACACACAC AGAGTCAACT CTAGAACAAG GGTCAAGTAG AAGGTTACTG
    ACAGAACAAG AAACACACAG AGAGTCAACT ACAGAACAAG GACAGCACAA AGGGTCAATA
    GAAGGACAAG GACCACGCAG AGTGTCAGTT TCAGAACAAG GATCAAGCAG AGAGTCAGTT
    GCAGAACAAG GGTCACGCAG AGAGTCTATT GCAGAACAAG ATCGACACAA AGGGTCAGTA
    GCAGAACAAG GATCACGCAG AATGTCAGCT GCAGAACAGG GATCACTCAG AGAGTCAGTA
    ATAGAAGAAC CATACCAAAA ATCAGAACAA GGACCTTATG GAGAGATAAT TTCAGAAGAG
    CAAGAAGACA TAGGCTCAAC TTCACAATCA AGAAAAGATA GTATCTTAAA AAGTACAAAA
    TATGGGGAAC CTATAACCTC TGAGTACATT GAAGTCCCTC TACAGGAAAA GAGGTCTTGG
    GAACAAACAT ATGAAGAGGA AATATTCCTG AGTTCTGAAC TGCAAGAGGA AGTTCCAACC
    TTAAGCAGAA AAGATCACTT TCCAGAAACT ACAAAAAAGG AAGTTCAGAA AGACAAGCCC
    TGTGAACCCA AGTCCCAAAA AATAGAAGGA AAGTCATGGT CAGGTGAATT TTTTACTTGT
    AACTGGAAAA TGAAGTATGT CACATTTGAA GATGAGGAAC AGGCAAACTT AATCTATGGT
    AACTCCAGGT TCACAGATTT ACACTCAATT ATCAGAAATA TTCAGTCTTG CAAGGAGGTA
    AAAGGCAGAA CTGCCTTCAA TGGAGTTTCA TTCAATCTGC TCCAGTTTGT GCAACTCCTG
    GAGACATTTG TTGGTGAAGA CGCTCCCTTG AGTGTCAGCG AGACTCTCAC CTCCTTTTTT
    AAGGAGGGCT ATGTTGAAAC AGAACAAGAG AAAATGAATG CCTTAGAACA GTTTAGCCAA
    AATGCTTTCC AAGTCCGACA GAGGCTTCTC CTAGAAGCCA TCTTTCAGAA GTGGGACAGT
    GATGGCTCAG GCTTCCTGGA TCTGAAGGAA GTTGATGAAC TCTTGTACAC ATACAAGGAG
    GGAATGGAAA AAGAATCTAT GAAGAAAGCT AAACTACACA TCCAATTTCC AAAGCCACAC
    CCTGGTCACG AAGTGAGATT GTCTTCAAAA CAATTTCAGA ATTACATAGA ATTGGTTGTG
    TCTGAACTCA GGGGCAATGA AGACCAAGTT CTGGAAAGTG TTGTGGAATT TCTGATGAAT
    GCTTTAGAGA GGAGCCACAT TGAGAGTCTG AGGAATTCTG CCAGGCGGAA ATGGCTGCAC
    CAAATCCAAT GTGCTGCAGA GACAAGTGGG GTGTCCCTAG AGCCGGTGTA TAGTGAGACC
    TTTAAGGCCC TCATGCAGGA TGCTGAAGCC CATGGAAATA AAAAGATCAG TGCTCACATT
    TCCCTCTTAG AAGAAAACCT ACTACTGCCT GAGAAAGGGA ATGTTCTATT GAGGAATGTG
    GCTTGTACCT TAGATGATGC TCAATTTGTA CTGAACAGAG TGCTCTACAG GGACATGAAA
    GGAATCAGCT TTACAGTAGT GGATGAAGGG AAGCCAATCC ATGTTCCCCA AGTTCAGTAC
    CATGGGAACA TCTTCTTCTG GAACCAGTCC CGTAATAAGC ATGATTATAA TGGTTCATTC
    CTGGCTCTGC CTCTTCAAGA TGCATATATG AGGATCTTTG GGGTCTTGGC TGTTGATACC
    CTTAGAGATC CCCACGAAAT AAACATCTTT CTACCTCATG AGATCAGATT CTATCAGGGT
    GTTGCTAATG TCTTTAGCAC TGCCTATCAC TACGTCCACA GCCGGGAGCA CATTCTGCAT
    ATTGTGATCA CTGGCATAGG CTGGCTTTAT GACGTCACAT CCAGCATCAC CTCCATCACT
    ACGTACTTTG TAGAGCCTAG CCCAGCCCAG GATTCAGACT ATGTTTTACG CAACATGATG
    GTTACAGGGC AGCTGGGTCT AACAGAAATC CACAAAAATC CTCCTACCAT CCACAGGAAG
    TCATGCATCT TCAGAGATTT CCTCTTTAAA TGTACTGACA GTTCAGAAGT TGTTCTGGCT
    TCTGCCTGTG GAGAAACGCA TATAGTAGTT CCACTTCGTG AGAGAACAGG AGAGGCTCTG
    GGAGTCCTCG ATTTTAACAT CGGCCAAAAT AGGATGTTGT TGTGTCAAGA ATATAAAGAT
    CTACAGAAAA TGATGAAAGT GGTCCAAGTG GCCTGCTATG AAATACTTGG CGAGTTCTCT
    GGAGAGATAA AGAAAAAATA TATCTTAGAG ATTGAAAATG TCAGGGAAGT CCAGCGGGCA
    GGAATTCTCT TCTTCCGAAT CATGCTGCTC GAGCTACAGG AAAGCATCCA ACTACTCAAT
    TCCATGGAAT TTGTGTCACT GTTGCTCTAT GACCATACTC TTGTAACAGA GCCAAATTCT
    CCTCAAGACA GCAAATCTAT GGAGTTGGAA GCCAACGTGA AACTAGTGCG TGACATCCTG
    AAGGCGGTTA TCTTGTTCTT TCATCCAGAG TTGGAATTTT CAAGTGACTT TGGAAGTTGG
    GATAAGTGTA AATTTTATGT TAACAAATAT TTAGTCAACA ATATTTGTGC CTTTGATCCA
    ACTGCCAAGC ATGTGGAAGT TAATGTACAG CTTATTGATG AATATATCAG AGATCATTCC
    CGAACTGAAG TATGGAAATT TGGTAATGTT GTCATTGAAC ATCTATACCA CTGGATACAC
    ATCTGTTCAG CTCTCATGAA GATAACCAAA CAACTAAATA GTGGTATTAC ACCTCCGTTG
    CCCTCCAAGA CTGACAATTA TATGTATGCA AAAATGCCAG GGGAAGGTTT GCAAGAGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu79652
    Clone ID Related Accession (Same CDS sequence) XM_011524768.2
    Accession Version XM_011524768.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGTTGCAG ATTCAGAATG TTCTTCAAGA ATTCTTTCAA
    AATCCAGATT TCAAGCTTGG ATCACACTGC AAACAACTGG ATATTACTGA CTCAACAGAA
    CCAAGATTGA ACAAAATGGA ATTTACAGAA TACATCTCTT CACATATTAA AGACTTGAAG
    AGTGAAATGT TTGAGGAACT TCTTAAGCAC CTTTGCCACT CTGCAGATGA ATTTCGGGAG
    GTCATAAAAG CTGACATGCG GAGGCAGATG TTCGCTGAAC TCTTCCTACA TTGTGACCAC
    GGGAAGGTAG GGTTTTTGGA TCGGCAGAGG ACATTGGCCC TGCTGGAATT GTTCTATGAC
    CATAGTTCAC AAATGCTTAG GAGTTTACTT CGAAACCCAC GACAATGGCC ATTCATTGAA
    TTTGAAGAGA TAAACTTGAC TGAGTTGTGG GGAGACATGG ATAATCAGAA ACACATTTAT
    GAAGGTTTTG ACAAAGTGCT CTTGGAGATG AATACATTAC TTTCTGCAAA TCATGCTAGC
    AAAACCCAAA GTAAATTATT AGAAAGTCCA GATCAACCTA AACTTAACGA ACAGAGAACA
    TCAACACCAT CACCAAACCC GCCAGAACAG CAGAGAGGAG TAACTGCAGA ACAAGGACCA
    CAAAGAATTT CAATTGAAGA ACAACAACAA GGCAAAAAGC CAACTGCAGA GCAAGAACTG
    TACATAGAAT CAGTAATAGA ACCAGGAACA CACACAGAGT CAACTCTAGA ACAAGGGTCA
    AGTAGAAGGT TACTGACAGA ACAAGAAACA CACAGAGAGT CAACTACAGA ACAAGGACAG
    CACAAAGGGT CAATAGAAGG ACAAGGACCA CGCAGAGTGT CAGTTTCAGA ACAAGGATCA
    AGCAGAGAGT CAGTTGCAGA ACAAGGGTCA CGCAGAGAGT CTATTGCAGA ACAAGATCGA
    CACAAAGGGT CAGTAGCAGA ACAAGGATCA CGCAGAATGT CAGCTGCAGA ACAGGGATCA
    CTCAGAGAGT CAGTAATAGA AGAACCATAC CAAAAATCAG AACAAGGACC TTATGGAGAG
    ATAATTTCAG AAGAGCAAGA AGACATAGGC TCAACTTCAC AATCAAGAAA AGATAGTATC
    TTAAAAAGTA CAAAATATGG GGAACCTATA ACCTCTGAGT ACATTGAAGT CCCTCTACAG
    GAAAAGAGGT CTTGGGAACA AACATATGAA GAGGAAATAT TCCTGAGTTC TGAACTGCAA
    GAGGAAGTTC CAACCTTAAG CAGAAAAGAT CACTTTCCAG AAACTACAAA AAAGGAAGTT
    CAGAAAGACA AGCCCTGTGA ACCCAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAATTTTTTA CTTGTAACTG GAAAATGAAG TATGTCACAT TTGAAGATGA GGAACAGGCA
    AACTTAATCT ATGGTAACTC CAGGTTCACA GATTTACACT CAATTATCAG AAATATTCAG
    TCTTGCAAGG AGGTAAAAGG CAGAACTGCC TTCAATGGAG TTTCATTCAA TCTGCTCCAG
    TTTGTGCAAC TCCTGGAGAC ATTTGTTGGT GAAGACGCTC CCTTGAGTGT CAGCGAGACT
    CTCACCTCCT TTTTTAAGGA GGGCTATGTT GAAACAGAAC AAGAGAAAAT GAATGCCTTA
    GAACAGTTTA GCCAAAATGC TTTCCAAGTC CGACAGAGGC TTCTCCTAGA AGCCATCTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC CTGGATCTGA AGGAAGTTGA TGAACTCTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCTATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACACCCTGG TCACGAAGTG AGATTGTCTT CAAAACAATT TCAGAATTAC
    ATAGAATTGG TTGTGTCTGA ACTCAGGGGC AATGAAGACC AAGTTCTGGA AAGTGTTGTG
    GAATTTCTGA TGAATGCTTT AGAGAGGAGC CACATTGAGA GTCTGAGGAA TTCTGCCAGG
    CGGAAATGGC TGCACCAAAT CCAATGTGCT GCAGAGACAA GTGGGGTGTC CCTAGAGCCG
    GTGTATAGTG AGACCTTTAA GGCCCTCATG CAGCTTTACA GTAGTGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011523070.1
    CDS193..3243
    Translation

    Target ORF information:

    RefSeq Version XM_011524768.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524768.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGTTGCAG ATTCAGAATG TTCTTCAAGA ATTCTTTCAA
    AATCCAGATT TCAAGCTTGG ATCACACTGC AAACAACTGG ATATTACTGA CTCAACAGAA
    CCAAGATTGA ACAAAATGGA ATTTACAGAA TACATCTCTT CACATATTAA AGACTTGAAG
    AGTGAAATGT TTGAGGAACT TCTTAAGCAC CTTTGCCACT CTGCAGATGA ATTTCGGGAG
    GTCATAAAAG CTGACATGCG GAGGCAGATG TTCGCTGAAC TCTTCCTACA TTGTGACCAC
    GGGAAGGTAG GGTTTTTGGA TCGGCAGAGG ACATTGGCCC TGCTGGAATT GTTCTATGAC
    CATAGTTCAC AAATGCTTAG GAGTTTACTT CGAAACCCAC GACAATGGCC ATTCATTGAA
    TTTGAAGAGA TAAACTTGAC TGAGTTGTGG GGAGACATGG ATAATCAGAA ACACATTTAT
    GAAGGTTTTG ACAAAGTGCT CTTGGAGATG AATACATTAC TTTCTGCAAA TCATGCTAGC
    AAAACCCAAA GTAAATTATT AGAAAGTCCA GATCAACCTA AACTTAACGA ACAGAGAACA
    TCAACACCAT CACCAAACCC GCCAGAACAG CAGAGAGGAG TAACTGCAGA ACAAGGACCA
    CAAAGAATTT CAATTGAAGA ACAACAACAA GGCAAAAAGC CAACTGCAGA GCAAGAACTG
    TACATAGAAT CAGTAATAGA ACCAGGAACA CACACAGAGT CAACTCTAGA ACAAGGGTCA
    AGTAGAAGGT TACTGACAGA ACAAGAAACA CACAGAGAGT CAACTACAGA ACAAGGACAG
    CACAAAGGGT CAATAGAAGG ACAAGGACCA CGCAGAGTGT CAGTTTCAGA ACAAGGATCA
    AGCAGAGAGT CAGTTGCAGA ACAAGGGTCA CGCAGAGAGT CTATTGCAGA ACAAGATCGA
    CACAAAGGGT CAGTAGCAGA ACAAGGATCA CGCAGAATGT CAGCTGCAGA ACAGGGATCA
    CTCAGAGAGT CAGTAATAGA AGAACCATAC CAAAAATCAG AACAAGGACC TTATGGAGAG
    ATAATTTCAG AAGAGCAAGA AGACATAGGC TCAACTTCAC AATCAAGAAA AGATAGTATC
    TTAAAAAGTA CAAAATATGG GGAACCTATA ACCTCTGAGT ACATTGAAGT CCCTCTACAG
    GAAAAGAGGT CTTGGGAACA AACATATGAA GAGGAAATAT TCCTGAGTTC TGAACTGCAA
    GAGGAAGTTC CAACCTTAAG CAGAAAAGAT CACTTTCCAG AAACTACAAA AAAGGAAGTT
    CAGAAAGACA AGCCCTGTGA ACCCAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAATTTTTTA CTTGTAACTG GAAAATGAAG TATGTCACAT TTGAAGATGA GGAACAGGCA
    AACTTAATCT ATGGTAACTC CAGGTTCACA GATTTACACT CAATTATCAG AAATATTCAG
    TCTTGCAAGG AGGTAAAAGG CAGAACTGCC TTCAATGGAG TTTCATTCAA TCTGCTCCAG
    TTTGTGCAAC TCCTGGAGAC ATTTGTTGGT GAAGACGCTC CCTTGAGTGT CAGCGAGACT
    CTCACCTCCT TTTTTAAGGA GGGCTATGTT GAAACAGAAC AAGAGAAAAT GAATGCCTTA
    GAACAGTTTA GCCAAAATGC TTTCCAAGTC CGACAGAGGC TTCTCCTAGA AGCCATCTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC CTGGATCTGA AGGAAGTTGA TGAACTCTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCTATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACACCCTGG TCACGAAGTG AGATTGTCTT CAAAACAATT TCAGAATTAC
    ATAGAATTGG TTGTGTCTGA ACTCAGGGGC AATGAAGACC AAGTTCTGGA AAGTGTTGTG
    GAATTTCTGA TGAATGCTTT AGAGAGGAGC CACATTGAGA GTCTGAGGAA TTCTGCCAGG
    CGGAAATGGC TGCACCAAAT CCAATGTGCT GCAGAGACAA GTGGGGTGTC CCTAGAGCCG
    GTGTATAGTG AGACCTTTAA GGCCCTCATG CAGCTTTACA GTAGTGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86835
    Clone ID Related Accession (Same CDS sequence) XM_017024591.1
    Accession Version XM_017024591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGGAGA ATGTTAAACA GAATAGAAAA CAAAGAGAAA GCATAGACAA AATCATAGTC 
    AAGGTGGCCA ACACACGGAA ACAGGCTCTG CAGGAGCAAT TCGATGAATG GATTCTAGAC
    CCTAAAGGAA TGATTCCTAA GTCAGTGTTG CAGATTCAGA ATGTTCTTCA AGAATTCTTT
    CAAAATCCAG ATTTCAAGCT TGGATCACAC TGCAAACAAC TGGATATTAC TGACTCAACA
    GAACCAAGAT TGAACAAAAT GGAATTTACA GAATACATCT CTTCACATAT TAAAGACTTG
    AAGAGTGAAA TGTTTGAGGA ACTTCTTAAG CACCTTTGCC ACTCTGCAGA TGAATTTCGG
    GAGGTCATAA AAGCTGACAT GCGGAGGCAG ATGTTCGCTG AACTCTTCCT ACATTGTGAC
    CACGGGAAGG TAGGGTTTTT GGATCGGCAG AGGACATTGG CCCTGCTGGA ATTGTTCTAT
    GACCATAGTT CACAAATGCT TAGGAGTTTA CTTCGAAACC CACGACAATG GCCATTCATT
    GAATTTGAAG AGATAAACTT GACTGAGTTG TGGGGAGACA TGGATAATCA GAAACACATT
    TATGAAGGTT TTGACAAAGT GCTCTTGGAG ATGAATACAT TACTTTCTGC AAATCATGCT
    AGCAAAACCC AAAGTAAATT ATTAGAAAGT CCAGATCAAC CTAAACTTAA CGAACAGAGA
    ACATCAACAC CATCACCAAA CCCGCCAGAA CAGCAGAGAG GAGTAACTGC AGAACAAGGA
    CCACAAAGAA TTTCAATTGA AGAACAACAA CAAGGCAAAA AGCCAACTGC AGAGCAAGAA
    CTGTACATAG AATCAGTAAT AGAACCAGGA ACACACACAG AGTCAACTCT AGAACAAGGG
    TCAAGTAGAA GGTTACTGAC AGAACAAGAA ACACACAGAG AGTCAACTAC AGAACAAGGA
    CAGCACAAAG GGTCAATAGA AGGACAAGGA CCACGCAGAG TGTCAGTTTC AGAACAAGGA
    TCAAGCAGAG AGTCAGTTGC AGAACAAGGG TCACGCAGAG AGTCTATTGC AGAACAAGAT
    CGACACAAAG GGTCAGTAGC AGAACAAGGA TCACGCAGAA TGTCAGCTGC AGAACAGGGA
    TCACTCAGAG AGTCAGTAAT AGAAGAACCA TACCAAAAAT CAGAACAAGG ACCTTATGGA
    GAGATAATTT CAGAAGAGCA AGAAGACATA GGCTCAACTT CACAATCAAG AAAAGATAGT
    ATCTTAAAAA GTACAAAATA TGGGGAACCT ATAACCTCTG AGTACATTGA AGTCCCTCTA
    CAGGAAAAGA GGTCTTGGGA ACAAACATAT GAAGAGGAAA TATTCCTGAG TTCTGAACTG
    CAAGAGGAAG TTCCAACCTT AAGCAGAAAA GATCACTTTC CAGAAACTAC AAAAAAGGAA
    GTTCAGAAAG ACAAGCCCTG TGAACCCAAG TCCCAAAAAA TAGAAGGAAA GTCATGGTCA
    GGTGAATTTT TTACTTGTAA CTGGAAAATG AAGTATGTCA CATTTGAAGA TGAGGAACAG
    GCAAACTTAA TCTATGGTAA CTCCAGGTTC ACAGATTTAC ACTCAATTAT CAGAAATATT
    CAGTCTTGCA AGGAGGTAAA AGGCAGAACT GCCTTCAATG GAGTTTCATT CAATCTGCTC
    CAGTTTGTGC AACTCCTGGA GACATTTGTT GGTGAAGACG CTCCCTTGAG TGTCAGCGAG
    ACTCTCACCT CCTTTTTTAA GGAGGGCTAT GTTGAAACAG AACAAGAGAA AATGAATGCC
    TTAGAACAGT TTAGCCAAAA TGCTTTCCAA GTCCGACAGA GGCTTCTCCT AGAAGCCATC
    TTTCAGAAGT GGGACAGTGA TGGCTCAGGC TTCCTGGATC TGAAGGAAGT TGATGAACTC
    TTGTACACAT ACAAGGAGGG AATGGAAAAA GAATCTATGA AGAAAGCTAA ACTACACATC
    CAATTTCCAA AGCCACACCC TGGTCACGAA GTGAGATTGT CTTCAAAACA ATTTCAGAAT
    TACATAGAAT TGGTTGTGTC TGAACTCAGG GGCAATGAAG ACCAAGTTCT GGAAAGTGTT
    GTGGAATTTC TGATGAATGC TTTAGAGAGG AGCCACATTG AGAGTCTGAG GAATTCTGCC
    AGGCGGAAAT GGCTGCACCA AATCCAATGT GCTGCAGAGA CAAGTGGGGT GTCCCTAGAG
    CCGGTGTATA GTGAGACCTT TAAGGCCCTC ATGCAGGATG CTGAAGCCCA TGGAAATAAA
    AAGATCAGTG CTCACATTTC CCTCTTAGAA GAAAACCTAC TACTGCCTGA GAAAGGGAAT
    GTTCTATTGA GGAATGTGGC TTGTACCTTA GATGATGCTC AATTTGTACT GAACAGAGTG
    CTCTACAGGG ACATGAAAGG AATCAGCTTT ACAGTAGTGG ATGAAGGGAA GCCAATCCAT
    GTTCCCCAAG TTCAGTACCA TGGGAACATC TTCTTCTGGA ACCAGTCCCG TAATAAGCAT
    GATTATAATG GTTCATTCCT GGCTCTGCCT CTTCAAGATG CATATATGAG GATCTTTGGG
    GTCTTGGCTG TTGATACCCT TAGAGATCCC CACGAAATAA ACATCTTTCT ACCTCATGAG
    ATCAGATTCT ATCAGGGTGT TGCTAATGTC TTTAGCACTG CCTATCACTA CGTCCACAGC
    CGGGAGCACA TTCTGCATAT TGTGATCACT GGCATAGGCT GGCTTTATGA CGTCACATCC
    AGCATCACCT CCATCACTAC GTACTTTGTA GAGCCTAGCC CAGCCCAGGA TTCAGACTAT
    GTTTTACGCA ACATGATGGT TACAGGGCAG CTGGGTCTAA CAGAAATCCA CAAAAATCCT
    CCTACCATCC ACAGGAAGTC ATGCATCTTC AGAGATTTCC TCTTTAAATG TACTGACAGT
    TCAGAAGTTG TTCTGGCTTC TGCCTGTGGA GAAACGCATA TAGTAGTTCC ACTTCGTGAG
    AGAACAGGAG AGGCTCTGGG AGTCCTCGAT TTTAACATCG GCCAAAATAG GATGTTGTTG
    TGTCAAGAAT ATAAAGATCT ACAGAAAATG ATGAAAGTGG TCCAAGTGGC CTGCTATGAA
    ATACTTGGCG AGTTCTCTGG AGAGATAAAG AAAAAATATA TCTTAGAGAT TGAAAATGTC
    AGGGAAGTCC AGCGGGCAGG AATTCTCTTC TTCCGAATCA TGCTGCTCGA GCTACAGGAA
    AGCATCCAAC TACTCAATTC CATGGAATTT GTGTCACTGT TGCTCTATGA CCATACTCTT
    GTAACAGAGC CAAATTCTCC TCAAGACAGC AAATCTATGG AGTTGGAAGC CAACGTGAAA
    CTAGTGCGTG ACATCCTGAA GGCGGTTATC TTGTTCTTTC ATCCAGAGTT GGAATTTTCA
    AGTGACTTTG GAAGTTGGGA TAAGTGTAAA TTTTATGTTA ACAAATATTT AGTCAACAAT
    ATTTGTGCCT TTGATCCAAC TGCCAAGCAT GTGGAAGTTA ATGTACAGCT TATTGATGAA
    TATATCAGAG ATCATTCCCG AACTGAAGTA TGGAAATTTG GTAATGTTGT CATTGAACAT
    CTATACCACT GGATACACAT CTGTTCAGCT CTCATGAAGA TAACCAAACA ACTAAATAGT
    GGTATTACAC CTCCGTTGCC CTCCAAGACT GACAATTATA TGTATGCAAA AATGCCAGGG
    GAAGGTTTGC AAGAGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880080.1
    CDS49..3789
    Translation

    Target ORF information:

    RefSeq Version XM_017024591.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGGAGA ATGTTAAACA GAATAGAAAA CAAAGAGAAA GCATAGACAA AATCATAGTC 
    AAGGTGGCCA ACACACGGAA ACAGGCTCTG CAGGAGCAAT TCGATGAATG GATTCTAGAC
    CCTAAAGGAA TGATTCCTAA GTCAGTGTTG CAGATTCAGA ATGTTCTTCA AGAATTCTTT
    CAAAATCCAG ATTTCAAGCT TGGATCACAC TGCAAACAAC TGGATATTAC TGACTCAACA
    GAACCAAGAT TGAACAAAAT GGAATTTACA GAATACATCT CTTCACATAT TAAAGACTTG
    AAGAGTGAAA TGTTTGAGGA ACTTCTTAAG CACCTTTGCC ACTCTGCAGA TGAATTTCGG
    GAGGTCATAA AAGCTGACAT GCGGAGGCAG ATGTTCGCTG AACTCTTCCT ACATTGTGAC
    CACGGGAAGG TAGGGTTTTT GGATCGGCAG AGGACATTGG CCCTGCTGGA ATTGTTCTAT
    GACCATAGTT CACAAATGCT TAGGAGTTTA CTTCGAAACC CACGACAATG GCCATTCATT
    GAATTTGAAG AGATAAACTT GACTGAGTTG TGGGGAGACA TGGATAATCA GAAACACATT
    TATGAAGGTT TTGACAAAGT GCTCTTGGAG ATGAATACAT TACTTTCTGC AAATCATGCT
    AGCAAAACCC AAAGTAAATT ATTAGAAAGT CCAGATCAAC CTAAACTTAA CGAACAGAGA
    ACATCAACAC CATCACCAAA CCCGCCAGAA CAGCAGAGAG GAGTAACTGC AGAACAAGGA
    CCACAAAGAA TTTCAATTGA AGAACAACAA CAAGGCAAAA AGCCAACTGC AGAGCAAGAA
    CTGTACATAG AATCAGTAAT AGAACCAGGA ACACACACAG AGTCAACTCT AGAACAAGGG
    TCAAGTAGAA GGTTACTGAC AGAACAAGAA ACACACAGAG AGTCAACTAC AGAACAAGGA
    CAGCACAAAG GGTCAATAGA AGGACAAGGA CCACGCAGAG TGTCAGTTTC AGAACAAGGA
    TCAAGCAGAG AGTCAGTTGC AGAACAAGGG TCACGCAGAG AGTCTATTGC AGAACAAGAT
    CGACACAAAG GGTCAGTAGC AGAACAAGGA TCACGCAGAA TGTCAGCTGC AGAACAGGGA
    TCACTCAGAG AGTCAGTAAT AGAAGAACCA TACCAAAAAT CAGAACAAGG ACCTTATGGA
    GAGATAATTT CAGAAGAGCA AGAAGACATA GGCTCAACTT CACAATCAAG AAAAGATAGT
    ATCTTAAAAA GTACAAAATA TGGGGAACCT ATAACCTCTG AGTACATTGA AGTCCCTCTA
    CAGGAAAAGA GGTCTTGGGA ACAAACATAT GAAGAGGAAA TATTCCTGAG TTCTGAACTG
    CAAGAGGAAG TTCCAACCTT AAGCAGAAAA GATCACTTTC CAGAAACTAC AAAAAAGGAA
    GTTCAGAAAG ACAAGCCCTG TGAACCCAAG TCCCAAAAAA TAGAAGGAAA GTCATGGTCA
    GGTGAATTTT TTACTTGTAA CTGGAAAATG AAGTATGTCA CATTTGAAGA TGAGGAACAG
    GCAAACTTAA TCTATGGTAA CTCCAGGTTC ACAGATTTAC ACTCAATTAT CAGAAATATT
    CAGTCTTGCA AGGAGGTAAA AGGCAGAACT GCCTTCAATG GAGTTTCATT CAATCTGCTC
    CAGTTTGTGC AACTCCTGGA GACATTTGTT GGTGAAGACG CTCCCTTGAG TGTCAGCGAG
    ACTCTCACCT CCTTTTTTAA GGAGGGCTAT GTTGAAACAG AACAAGAGAA AATGAATGCC
    TTAGAACAGT TTAGCCAAAA TGCTTTCCAA GTCCGACAGA GGCTTCTCCT AGAAGCCATC
    TTTCAGAAGT GGGACAGTGA TGGCTCAGGC TTCCTGGATC TGAAGGAAGT TGATGAACTC
    TTGTACACAT ACAAGGAGGG AATGGAAAAA GAATCTATGA AGAAAGCTAA ACTACACATC
    CAATTTCCAA AGCCACACCC TGGTCACGAA GTGAGATTGT CTTCAAAACA ATTTCAGAAT
    TACATAGAAT TGGTTGTGTC TGAACTCAGG GGCAATGAAG ACCAAGTTCT GGAAAGTGTT
    GTGGAATTTC TGATGAATGC TTTAGAGAGG AGCCACATTG AGAGTCTGAG GAATTCTGCC
    AGGCGGAAAT GGCTGCACCA AATCCAATGT GCTGCAGAGA CAAGTGGGGT GTCCCTAGAG
    CCGGTGTATA GTGAGACCTT TAAGGCCCTC ATGCAGGATG CTGAAGCCCA TGGAAATAAA
    AAGATCAGTG CTCACATTTC CCTCTTAGAA GAAAACCTAC TACTGCCTGA GAAAGGGAAT
    GTTCTATTGA GGAATGTGGC TTGTACCTTA GATGATGCTC AATTTGTACT GAACAGAGTG
    CTCTACAGGG ACATGAAAGG AATCAGCTTT ACAGTAGTGG ATGAAGGGAA GCCAATCCAT
    GTTCCCCAAG TTCAGTACCA TGGGAACATC TTCTTCTGGA ACCAGTCCCG TAATAAGCAT
    GATTATAATG GTTCATTCCT GGCTCTGCCT CTTCAAGATG CATATATGAG GATCTTTGGG
    GTCTTGGCTG TTGATACCCT TAGAGATCCC CACGAAATAA ACATCTTTCT ACCTCATGAG
    ATCAGATTCT ATCAGGGTGT TGCTAATGTC TTTAGCACTG CCTATCACTA CGTCCACAGC
    CGGGAGCACA TTCTGCATAT TGTGATCACT GGCATAGGCT GGCTTTATGA CGTCACATCC
    AGCATCACCT CCATCACTAC GTACTTTGTA GAGCCTAGCC CAGCCCAGGA TTCAGACTAT
    GTTTTACGCA ACATGATGGT TACAGGGCAG CTGGGTCTAA CAGAAATCCA CAAAAATCCT
    CCTACCATCC ACAGGAAGTC ATGCATCTTC AGAGATTTCC TCTTTAAATG TACTGACAGT
    TCAGAAGTTG TTCTGGCTTC TGCCTGTGGA GAAACGCATA TAGTAGTTCC ACTTCGTGAG
    AGAACAGGAG AGGCTCTGGG AGTCCTCGAT TTTAACATCG GCCAAAATAG GATGTTGTTG
    TGTCAAGAAT ATAAAGATCT ACAGAAAATG ATGAAAGTGG TCCAAGTGGC CTGCTATGAA
    ATACTTGGCG AGTTCTCTGG AGAGATAAAG AAAAAATATA TCTTAGAGAT TGAAAATGTC
    AGGGAAGTCC AGCGGGCAGG AATTCTCTTC TTCCGAATCA TGCTGCTCGA GCTACAGGAA
    AGCATCCAAC TACTCAATTC CATGGAATTT GTGTCACTGT TGCTCTATGA CCATACTCTT
    GTAACAGAGC CAAATTCTCC TCAAGACAGC AAATCTATGG AGTTGGAAGC CAACGTGAAA
    CTAGTGCGTG ACATCCTGAA GGCGGTTATC TTGTTCTTTC ATCCAGAGTT GGAATTTTCA
    AGTGACTTTG GAAGTTGGGA TAAGTGTAAA TTTTATGTTA ACAAATATTT AGTCAACAAT
    ATTTGTGCCT TTGATCCAAC TGCCAAGCAT GTGGAAGTTA ATGTACAGCT TATTGATGAA
    TATATCAGAG ATCATTCCCG AACTGAAGTA TGGAAATTTG GTAATGTTGT CATTGAACAT
    CTATACCACT GGATACACAT CTGTTCAGCT CTCATGAAGA TAACCAAACA ACTAAATAGT
    GGTATTACAC CTCCGTTGCC CTCCAAGACT GACAATTATA TGTATGCAAA AATGCCAGGG
    GAAGGTTTGC AAGAGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86836
    Clone ID Related Accession (Same CDS sequence) XM_017024592.1
    Accession Version XM_017024592.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGATTCAG AATGTTCTTC AAGAATTCTT TCAAAATCCA
    GATTTCAAGC TTGGATCACA CTGCAAACAA CTGGATATTA CTGACTCAAC AGAACCAAGA
    TTGAACAAAA TGGAATTTAC AGAATACATC TCTTCACATA TTAAAGACTT GAAGAGTGAA
    ATGTTTGAGG AACTTCTTAA GCACCTTTGC CACTCTGCAG ATGAATTTCG GGAGGTCATA
    AAAGCTGACA TGCGGAGGCA GATGTTCGCT GAACTCTTCC TACATTGTGA CCACGGGAAG
    GTAGGGTTTT TGGATCGGCA GAGGACATTG GCCCTGCTGG AATTGTTCTA TGACCATAGT
    TCACAAATGC TTAGGAGTTT ACTTCGAAAC CCACGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAAACT TGACTGAGTT GTGGGGAGAC ATGGATAATC AGAAACACAT TTATGAAGGT
    TTTGACAAAG TGCTCTTGGA GATGAATACA TTACTTTCTG CAAATCATGC TAGCAAAACC
    CAAAGTAAAT TATTAGAAAG TCCAGATCAA CCTAAACTTA ACGAACAGAG AACATCAACA
    CCATCACCAA ACCCGCCAGA ACAGCAGAGA GGAGTAACTG CAGAACAAGG ACCACAAAGA
    ATTTCAATTG AAGAACAACA ACAAGGCAAA AAGCCAACTG CAGAGCAAGA ACTGTACATA
    GAATCAGTAA TAGAACCAGG AACACACACA GAGTCAACTC TAGAACAAGG GTCAAGTAGA
    AGGTTACTGA CAGAACAAGA AACACACAGA GAGTCAACTA CAGAACAAGG ACAGCACAAA
    GGGTCAATAG AAGGACAAGG ACCACGCAGA GTGTCAGTTT CAGAACAAGG ATCAAGCAGA
    GAGTCAGTTG CAGAACAAGG GTCACGCAGA GAGTCTATTG CAGAACAAGA TCGACACAAA
    GGGTCAGTAG CAGAACAAGG ATCACGCAGA ATGTCAGCTG CAGAACAGGG ATCACTCAGA
    GAGTCAGTAA TAGAAGAACC ATACCAAAAA TCAGAACAAG GACCTTATGG AGAGATAATT
    TCAGAAGAGC AAGAAGACAT AGGCTCAACT TCACAATCAA GAAAAGATAG TATCTTAAAA
    AGTACAAAAT ATGGGGAACC TATAACCTCT GAGTACATTG AAGTCCCTCT ACAGGAAAAG
    AGGTCTTGGG AACAAACATA TGAAGAGGAA ATATTCCTGA GTTCTGAACT GCAAGAGGAA
    GTTCCAACCT TAAGCAGAAA AGATCACTTT CCAGAAACTA CAAAAAAGGA AGTTCAGAAA
    GACAAGCCCT GTGAACCCAA GTCCCAAAAA ATAGAAGGAA AGTCATGGTC AGGTGAATTT
    TTTACTTGTA ACTGGAAAAT GAAGTATGTC ACATTTGAAG ATGAGGAACA GGCAAACTTA
    ATCTATGGTA ACTCCAGGTT CACAGATTTA CACTCAATTA TCAGAAATAT TCAGTCTTGC
    AAGGAGGTAA AAGGCAGAAC TGCCTTCAAT GGAGTTTCAT TCAATCTGCT CCAGTTTGTG
    CAACTCCTGG AGACATTTGT TGGTGAAGAC GCTCCCTTGA GTGTCAGCGA GACTCTCACC
    TCCTTTTTTA AGGAGGGCTA TGTTGAAACA GAACAAGAGA AAATGAATGC CTTAGAACAG
    GTCTCACTCT GTCGCCAGGC TGGAGTGCAG TGGCGCAACT GTAGCTCACT GCAGCCTCCA
    ACTCCCGGAC TCAAGCGATC ATCCCATCAC AGACTCCTGA GCAACTGGGA CTACAGGGAA
    AGAAGGAAGT TTTTTCCTCT TGAGACCAAA TTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880081.1
    CDS193..2931
    Translation

    Target ORF information:

    RefSeq Version XM_017024592.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATGAGT CAGCATCTCA AGAGGAACTC AGACCTGCTC AGGAAAACAG AAAAGAAGAC 
    AAAGAGAGGA AATGGAACTT AACTGAAGTG AAAGAGCTTC ATGAGACCTT ACAGAGTGTG
    CCAGACGTTC CTGTAAAAGA GGACACCAAC AGTGTGGTGG AGAAAGCAAT GGATGAAATC
    AAATCCCAAG AATTAAACCT GGAGGGGCAG CGAAAAATTT CACCTGGTTC AATAAAGGAC
    TCTAAAACTG AAGCCTCAGG TAATATTGCA ATTAGAAAAT CTGCAAAAGT GATTTTTGCT
    TTAGATGAAA CTGAACTGAA ATCAAAGCCA GAGCACACAT GGAAGAAAAA CCTTTTTGAA
    AGAATGGAGG CAAGAGCCCA AGCAATGCAG CAGAAAATAA TAGATAAGGA AAATCTGAAG
    AAGGAACTAG AAAAAAAGGC TGAAAAAAAA CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATACTG ACAGCATGAC ACTGAATAAT ACTGCATATT TGCTTGACAA ACTTCTACCC
    ACCTTAGTTC CTGGAGTAGA AAACATGTTA ACTCAAGTAG AAAAGAAGAA GGTTTTGACA
    GAAGCTGATA CTCCAAGCAA GTTTGACCCA ATTAATTATT TGGGGGAATA TTTAATAAGA
    AACAATCCTA ATTATATCAA AGACCCAGGA ATGTCTGGTT ACCAGAGGTT GATGAAAGAA
    GTCACAGAAG ACCTGAAGAT ATATGTTCCT GACACTATCT GCAACAGAGT ATCCAAGATG
    AAGGAGAATG TTAAACAGAA TAGAAAACAA AGAGAAAGCA TAGACAAAAT CATAGTCAAG
    GTGGCCAACA CACGGAAACA GGCTCTGCAG GAGCAATTCG ATGAATGGAT TCTAGACCCT
    AAAGGAATGA TTCCTAAGTC AGTGATTCAG AATGTTCTTC AAGAATTCTT TCAAAATCCA
    GATTTCAAGC TTGGATCACA CTGCAAACAA CTGGATATTA CTGACTCAAC AGAACCAAGA
    TTGAACAAAA TGGAATTTAC AGAATACATC TCTTCACATA TTAAAGACTT GAAGAGTGAA
    ATGTTTGAGG AACTTCTTAA GCACCTTTGC CACTCTGCAG ATGAATTTCG GGAGGTCATA
    AAAGCTGACA TGCGGAGGCA GATGTTCGCT GAACTCTTCC TACATTGTGA CCACGGGAAG
    GTAGGGTTTT TGGATCGGCA GAGGACATTG GCCCTGCTGG AATTGTTCTA TGACCATAGT
    TCACAAATGC TTAGGAGTTT ACTTCGAAAC CCACGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAAACT TGACTGAGTT GTGGGGAGAC ATGGATAATC AGAAACACAT TTATGAAGGT
    TTTGACAAAG TGCTCTTGGA GATGAATACA TTACTTTCTG CAAATCATGC TAGCAAAACC
    CAAAGTAAAT TATTAGAAAG TCCAGATCAA CCTAAACTTA ACGAACAGAG AACATCAACA
    CCATCACCAA ACCCGCCAGA ACAGCAGAGA GGAGTAACTG CAGAACAAGG ACCACAAAGA
    ATTTCAATTG AAGAACAACA ACAAGGCAAA AAGCCAACTG CAGAGCAAGA ACTGTACATA
    GAATCAGTAA TAGAACCAGG AACACACACA GAGTCAACTC TAGAACAAGG GTCAAGTAGA
    AGGTTACTGA CAGAACAAGA AACACACAGA GAGTCAACTA CAGAACAAGG ACAGCACAAA
    GGGTCAATAG AAGGACAAGG ACCACGCAGA GTGTCAGTTT CAGAACAAGG ATCAAGCAGA
    GAGTCAGTTG CAGAACAAGG GTCACGCAGA GAGTCTATTG CAGAACAAGA TCGACACAAA
    GGGTCAGTAG CAGAACAAGG ATCACGCAGA ATGTCAGCTG CAGAACAGGG ATCACTCAGA
    GAGTCAGTAA TAGAAGAACC ATACCAAAAA TCAGAACAAG GACCTTATGG AGAGATAATT
    TCAGAAGAGC AAGAAGACAT AGGCTCAACT TCACAATCAA GAAAAGATAG TATCTTAAAA
    AGTACAAAAT ATGGGGAACC TATAACCTCT GAGTACATTG AAGTCCCTCT ACAGGAAAAG
    AGGTCTTGGG AACAAACATA TGAAGAGGAA ATATTCCTGA GTTCTGAACT GCAAGAGGAA
    GTTCCAACCT TAAGCAGAAA AGATCACTTT CCAGAAACTA CAAAAAAGGA AGTTCAGAAA
    GACAAGCCCT GTGAACCCAA GTCCCAAAAA ATAGAAGGAA AGTCATGGTC AGGTGAATTT
    TTTACTTGTA ACTGGAAAAT GAAGTATGTC ACATTTGAAG ATGAGGAACA GGCAAACTTA
    ATCTATGGTA ACTCCAGGTT CACAGATTTA CACTCAATTA TCAGAAATAT TCAGTCTTGC
    AAGGAGGTAA AAGGCAGAAC TGCCTTCAAT GGAGTTTCAT TCAATCTGCT CCAGTTTGTG
    CAACTCCTGG AGACATTTGT TGGTGAAGAC GCTCCCTTGA GTGTCAGCGA GACTCTCACC
    TCCTTTTTTA AGGAGGGCTA TGTTGAAACA GAACAAGAGA AAATGAATGC CTTAGAACAG
    GTCTCACTCT GTCGCCAGGC TGGAGTGCAG TGGCGCAACT GTAGCTCACT GCAGCCTCCA
    ACTCCCGGAC TCAAGCGATC ATCCCATCAC AGACTCCTGA GCAACTGGGA CTACAGGGAA
    AGAAGGAAGT TTTTTCCTCT TGAGACCAAA TTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Genome-wide meta-analysis identifies six novel loci associated with habitual coffee consumption.
    Molecular psychiatry20(5)647-656(2015 May)
    ,Cornelis MC,Byrne EM,Esko T,Nalls MA,Ganna A,Paynter N,Monda KL,Amin N,Fischer K,Renstrom F,Ngwa JS,Huikari V,Cavadino A,Nolte IM,Teumer A,Yu K,Marques-Vidal P,Rawal R,Manichaikul A,Wojczynski MK,Vink JM,Zhao JH,Burlutsky G,Lahti J,Mikkil? V,Lemaitre RN,Eriksson J,Musani SK,Tanaka T,Geller F,Luan J,Hui J,M?gi R,Dimitriou M,Garcia ME,Ho WK,Wright MJ,Rose LM,Magnusson PK,Pedersen NL,Couper D,Oostra BA,Hofman A,Ikram MA,Tiemeier HW,Uitterlinden AG,van Rooij FJ,Barroso I,Johansson I,Xue L,Kaakinen M,Milani L,Power C,Snieder H,Stolk RP,Baumeister SE,Biffar R,Gu F,Bastardot F,Kutalik Z,Jacobs DR Jr,Forouhi NG,Mihailov E,Lind L,Lindgren C,Micha?lsson K,Morris A,Jensen M,Khaw KT,Luben RN,Wang JJ,M?nnist? S,Per?l? MM,K?h?nen M,Lehtim?ki T,Viikari J,Mozaffarian D,Mukamal K,Psaty BM,D?ring A,Heath AC,Montgomery GW,Dahmen N,Carithers T,Tucker KL,Ferrucci L,Boyd HA,Melbye M,Treur JL,Mellstr?m D,Hottenga JJ,Prokopenko I,T?njes A,Deloukas P,Kanoni S,Lorentzon M,Houston DK,Liu Y,Danesh J,Rasheed A,Mason MA,Zonderman AB,Franke L,Kristal BS,,,,Karjalainen J,Reed DR,Westra HJ,Evans MK,Saleheen D,Harris TB,Dedoussis G,Curhan G,Stumvoll M,Beilby J,Pasquale LR,Feenstra B,Bandinelli S,Ordovas JM,Chan AT,Peters U,Ohlsson C,Gieger C,Martin NG,Waldenberger M,Siscovick DS,Raitakari O,Eriksson JG,Mitchell P,Hunter DJ,Kraft P,Rimm EB,Boomsma DI,Borecki IB,Loos RJ,Wareham NJ,Vollenweider P,Caporaso N,Grabe HJ,Neuhouser ML,Wolffenbuttel BH,Hu FB,Hypp?nen E,J?rvelin MR,Cupples LA,Franks PW,Ridker PM,van Duijn CM,Heiss G,Metspalu A,North KE,Ingelsson E,Nettleton JA,van Dam RM,Chasman DI


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