EFCAB5 cDNA ORF clone, Canis lupus familiaris(dog)

The following EFCAB5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFCAB5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe12276 XM_005624898.2
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe12277 XM_005624901.3
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12277 XM_005624900.2
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12277 XM_022423417.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12277 XM_022423407.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12277 XM_005624899.3
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12278 XM_005624902.3
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12278 XM_022423410.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12279 XM_022423418.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe12279 XM_005624903.2
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe290829 XM_022423408.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe290830 XM_022423409.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe290831 XM_022423411.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe290832 XM_022423412.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe290833 XM_022423413.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe290834 XM_022423414.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe290835 XM_022423415.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe290836 XM_022423416.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe290837 XM_022423419.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe290838 XM_022423420.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe290839 XM_022423421.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe290840 XM_022423422.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe290841 XM_022423423.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe290842 XM_022423424.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe290843 XM_022423425.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe290844 XM_022423426.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OCe12277 XM_005624899.2 Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12277 XM_005624901.2 Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe12278 XM_005624902.2 Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe12276
    Clone ID Related Accession (Same CDS sequence) XM_005624898.2
    Accession Version XM_005624898.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGAATAC TGAACGCATC CAGGAAATGG GTGTGGGTGT TTGTGCATTT GAAGAAGAGT 
    CACCTCTTCC AGACTTTATG GAATGACTTT GTGGCCCAGA TGAGTGAGTC AGCATTTCAA
    GAGGAACCTA AACCTGCTCA GGAAAATGGA AAAGAAGACA AAGAAAACAA ACCCGAAGAA
    ACTGAAGTGA AAGAACCTAC TGAGAGTTCA CAGGGTGTAC CAGGCAGTTC TGCATTAGAC
    ACCAGCAGCA ATGTGGCCGA AACAGAAGCA GATGAAATCA AATCCCAAGA AATACACCTG
    GAAGGGCAGT CAAAAACTTC AGTTTGTTCA ATAAAGGACT CTAGCACTAG AGTCTCAAAG
    AATTTTGTGG TTAGAAAACC TGCAAAAGTG ATCTTCAATT TAGATCCAAC TGCGCTGAAT
    TCAAAGCTAG AACAGCCATG GAAGAAAAAT TTTTTTGAAA GATTGGAGGC AAAAGCCCAG
    AAAATGCAGC AGAAAATAAT AGATAAGGAT AACCTGAATA AAGAACTAGA AAAAAAGGCT
    GAAAAAAACC TCCCTAGGGA TAATTTGGCC AAAGAGTGGT TTAATGCTGA AAGCATAACA
    TTGGACAGTC GTGCATATTT GCTGGACAAA CTTCTACCTA CCTTAGTTCC TGGCGTGGAA
    AAAATGTTAA TGCAAGTGGA AAAGAAGAAG CTTTGGGCAG AAGGTGATAT TACAACCAAG
    TTTAATCCAA ATAATCATTT GGGGGAATAC TTAATGAGAA ACAATCCTTA TTATATAAAA
    GATTCAGGAA TGTCTGGTTA TCAAAGAGTG ATGAGGGATG TCACAGAAGA CCTGAAGATA
    CATGTTCCCA ACACTGTTGG CAACAGAGTA ACCAAGATGA AGGAGAGTAT TAAACAGAAA
    CGAGAACAAA GACAATTCAT AAAAGAAGTC AAAGTCAGGG TGACCAAAAC ACGGAAGCAG
    GCTCTGCAGG AGCAATTCAG TGAATGGATT CTAGACCCCA AAGGAACAAT TCCTATGGTT
    GTGATTCAGA ATGTTCTTCA TGAATTTTTT CAAAATCCAG ATTTACAGCT TGAAATATGT
    TGCAAACCAA TGGATATTGT TGACTCGATG GGACCAAGAT TGAACAAAAC AGAATTTATA
    GAATATGTCT CTTCATATGT TGCAGACTTT AAAAATGAAA TGTTCGAGAA ACTCCTTAAG
    CACTTTTGTC ATTGTGCAGA TGAATTCCGG GAGGTTATAA AAACAGACAT GCGGAGGCAG
    ATATTTGCTG AACTCTTCCT GCATTGTGAC CATGGGAAGG TAGGATTTTT GGATCGGCAG
    AGGACATTGG CTTTGTTGGA AACATTCTAT GACCAAAGTT CAAAAATGCT TAGAAGTTTG
    CTACGAAACC CAAGACAATG GCCATTCATT GAATTTGAAG AGATAGATTT GCCTGAGTTC
    TGGGGAGACA TGGATAATCA GAAACATATT TATGAAGACT TTGACAAAGT GCTCTTAGAG
    ATGAATGCAC AACTTTCTGA AAAATATGCT AGCAAAACCC AAAGCAAATT GTTAGAAACT
    CCAGAAGATC AACATTATCA TGATGAACAT AGTGAATCAA CACTACCAGA ACAGCAGAGA
    GGGACAACTG CAGAACAAGA ACGAAACAGA ATTTTAAGCA AGGAACAAGA ACAAGGCAGA
    GAGTTAACTG GAGAAAAAGA ACTAGACAAA GACTCAGTAA CAGAACAAGA AACCCACACA
    GGGTCAACTC CAGAACAGGG ATCAAGTAGA GAGTTAATAA TAGAACAAAG ACTACATAGT
    GACTCAGTAA TAGAACAAGA GCCACCACAA AGCATGTCTT CTTCAGAACA AGGAGTCAAC
    ATGGGAACAA CTGCAGAACA AGGACTCTAC AGAGAATCAA TAGCAGCACA AGGACAACAC
    AAACGGTCAG ACACAGAACA AGGACCACAC AGAGGGTCAA CCTCAGAACA AGGATCACGC
    AGACAGTCAG TAATAGAACA AGAACCACTC AAAGGGTCAA CTGCAGAACA AGAGCCATCC
    AGAGAGATAT TCCCAGAAAG ACAACAGGAT TCAACCTTAC AATCAGGAAG AGATAGTATT
    TTCAGAGAAA GTAAAATATT TAGGAATGCT ACTTCCTTTG AGTACACTGA AATCTCCCCA
    CAGAAAGAGA GGACTCAGGA ACAAACCTAT GAAAAAGAAC TATTTGTGAG TTCTGAGCTG
    CAAGAAGAAG TCTCAACATC ACGAAGAAAA GATCATCTTT CAGAAACTAC AAAAAAGGAA
    ATTCAGAAAG ATCCATCCTG TGAAAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAACTTCTTA CTTGTAACTG GAAGATGAAT TATTTCAAAT GTGAAGATGA GGAGCAAGCC
    AACTTACTCT ACAGTGATAA CAGGTTCACA GACCTACACT CAATTATCAG AAATATTCAG
    TCTTACAAGG AAGTAAAAGG TAGAAGTGCT TTCAATGGAG TTTCCTTCAA TCTGCTCCAG
    TTTGTGCAAC TTTTGGAGAC ATTTGTTGGT GAAGATGTCC CCTTGAGTGT CAGTGAGACT
    CTCACAACCT TTTTTAAGAG GGGCTATGTT GAAACAAAAG AAGAGAAAAT AAGTGGCTTA
    GAACAGACTA GACAAAATGC TTCCAGAGTC CGACAGGAAC TTCTCCTAGA AGCCCTGTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC TTGGATCTGA AGGAAATTGA TGAACTTTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCCATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACAACCTGA TCAAGAAGTG AGACTGTCTT CAAAACAATT TCAGAAATAC
    ATAGAATTGG TTGTCTCTGA ACTCAGGGGC AATGAAGATC ATGTTCTGGA AAGTATTGTG
    GAGTTTCTGG TGAATACTTT AGAAAGGAGC CATACTGAGA ATCTGAGGAA TTGTGCCAGA
    CGAAAGTGGC TATACCAAAT CCAACATGCT GCAGAGACAA GTGGAGTATC CCTGGAGCCT
    GTGTACACTG AAACCTTTAA GGCCCTCACC CAGGATGCTG AAGCCCATGG AAATAAGAAG
    ATCTGTGCTC ATATTTCCCT CTTAGAAGAA AACCTACTAC TGCCTGATAG AGGGGAAGTT
    GTATTAAGGA ACGTAGCTTG TACTTTAGAT GATGCTCCAT TTGTACTGAA CAAAGTGCTC
    TACAGGGACA TGAAGGGAAT CAGCTTTACA GTAGTGGATG AAGGTAAGCC AATACACGTC
    CCCCAAGTTC AGCACCATGG GAACGTCTTT TTCTGGAACC ATTCCCTCAA GAAGAGTGAT
    CAAAATGGAT CATTCCTGGC CCTGCCTCTT CAGGATGCAT ACATGAGGAT CTTTGGGGTC
    ATGGCTGTGG ACACCCTAAG AGATAAAAAA AAAATAAACA TCTTTGTACC TCATGAGATC
    AGATTCTATC AGGGTGTTGC TAATGTCTTT AGCACTGCCT ATCACTATGT CCACAGCAGG
    GAGCACATCC TGCATGTTGT GATTACTGGC ATTGGCTGGC TCTGTGACAT CACATCTGAC
    ATCACCGCCA TCACTACATA CTTCATTGAG CCTGGCCTAA TGCAGGATTC AGACTATGTT
    TTACGCAATA TGATGGTTAC CAGACACCTG GGTCTAACAG AAATCCACAG GAACCCCCCT
    ATAATTTTCA GGAAGACATG CATCTTCAGA GACTTCCTCT TTAAATGTAC AGACAGTTCC
    GAAGTTGTCT TGGCCTCTGT CTGTGGAGAA AACCACATAC TAATTCCACT CCGTGAGAGA
    ACAGGAAAAG CTTTGGGAGT TCTTGATTTT AACATTGGCA AGAGTAAAAT GTTATTGTAT
    CGAGAATTTA AAGACCTACA GAAAATGATG AAAGTGGTCC AAGCTGCCTG CTACGAAATA
    CTGGGCGAAT TATCTGGAGA AATAAAGAAA AACTATATCC TAGAGATTGA AAATGTAGGA
    GAAGTCCAGC GGGCAGGAGT TCTCTTTTTC CGAATCATGC TACAAGAGCT GCAAGAAAGC
    CTCCGATTGC TTACCTCCAT AGACTTTGTA TCACTGTTGA TCTATGATTA TCATATTCCA
    GCAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624955.1
    CDS414..4994
    Translation

    Target ORF information:

    RefSeq Version XM_005624898.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624898.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGAATAC TGAACGCATC CAGGAAATGG GTGTGGGTGT TTGTGCATTT GAAGAAGAGT 
    CACCTCTTCC AGACTTTATG GAATGACTTT GTGGCCCAGA TGAGTGAGTC AGCATTTCAA
    GAGGAACCTA AACCTGCTCA GGAAAATGGA AAAGAAGACA AAGAAAACAA ACCCGAAGAA
    ACTGAAGTGA AAGAACCTAC TGAGAGTTCA CAGGGTGTAC CAGGCAGTTC TGCATTAGAC
    ACCAGCAGCA ATGTGGCCGA AACAGAAGCA GATGAAATCA AATCCCAAGA AATACACCTG
    GAAGGGCAGT CAAAAACTTC AGTTTGTTCA ATAAAGGACT CTAGCACTAG AGTCTCAAAG
    AATTTTGTGG TTAGAAAACC TGCAAAAGTG ATCTTCAATT TAGATCCAAC TGCGCTGAAT
    TCAAAGCTAG AACAGCCATG GAAGAAAAAT TTTTTTGAAA GATTGGAGGC AAAAGCCCAG
    AAAATGCAGC AGAAAATAAT AGATAAGGAT AACCTGAATA AAGAACTAGA AAAAAAGGCT
    GAAAAAAACC TCCCTAGGGA TAATTTGGCC AAAGAGTGGT TTAATGCTGA AAGCATAACA
    TTGGACAGTC GTGCATATTT GCTGGACAAA CTTCTACCTA CCTTAGTTCC TGGCGTGGAA
    AAAATGTTAA TGCAAGTGGA AAAGAAGAAG CTTTGGGCAG AAGGTGATAT TACAACCAAG
    TTTAATCCAA ATAATCATTT GGGGGAATAC TTAATGAGAA ACAATCCTTA TTATATAAAA
    GATTCAGGAA TGTCTGGTTA TCAAAGAGTG ATGAGGGATG TCACAGAAGA CCTGAAGATA
    CATGTTCCCA ACACTGTTGG CAACAGAGTA ACCAAGATGA AGGAGAGTAT TAAACAGAAA
    CGAGAACAAA GACAATTCAT AAAAGAAGTC AAAGTCAGGG TGACCAAAAC ACGGAAGCAG
    GCTCTGCAGG AGCAATTCAG TGAATGGATT CTAGACCCCA AAGGAACAAT TCCTATGGTT
    GTGATTCAGA ATGTTCTTCA TGAATTTTTT CAAAATCCAG ATTTACAGCT TGAAATATGT
    TGCAAACCAA TGGATATTGT TGACTCGATG GGACCAAGAT TGAACAAAAC AGAATTTATA
    GAATATGTCT CTTCATATGT TGCAGACTTT AAAAATGAAA TGTTCGAGAA ACTCCTTAAG
    CACTTTTGTC ATTGTGCAGA TGAATTCCGG GAGGTTATAA AAACAGACAT GCGGAGGCAG
    ATATTTGCTG AACTCTTCCT GCATTGTGAC CATGGGAAGG TAGGATTTTT GGATCGGCAG
    AGGACATTGG CTTTGTTGGA AACATTCTAT GACCAAAGTT CAAAAATGCT TAGAAGTTTG
    CTACGAAACC CAAGACAATG GCCATTCATT GAATTTGAAG AGATAGATTT GCCTGAGTTC
    TGGGGAGACA TGGATAATCA GAAACATATT TATGAAGACT TTGACAAAGT GCTCTTAGAG
    ATGAATGCAC AACTTTCTGA AAAATATGCT AGCAAAACCC AAAGCAAATT GTTAGAAACT
    CCAGAAGATC AACATTATCA TGATGAACAT AGTGAATCAA CACTACCAGA ACAGCAGAGA
    GGGACAACTG CAGAACAAGA ACGAAACAGA ATTTTAAGCA AGGAACAAGA ACAAGGCAGA
    GAGTTAACTG GAGAAAAAGA ACTAGACAAA GACTCAGTAA CAGAACAAGA AACCCACACA
    GGGTCAACTC CAGAACAGGG ATCAAGTAGA GAGTTAATAA TAGAACAAAG ACTACATAGT
    GACTCAGTAA TAGAACAAGA GCCACCACAA AGCATGTCTT CTTCAGAACA AGGAGTCAAC
    ATGGGAACAA CTGCAGAACA AGGACTCTAC AGAGAATCAA TAGCAGCACA AGGACAACAC
    AAACGGTCAG ACACAGAACA AGGACCACAC AGAGGGTCAA CCTCAGAACA AGGATCACGC
    AGACAGTCAG TAATAGAACA AGAACCACTC AAAGGGTCAA CTGCAGAACA AGAGCCATCC
    AGAGAGATAT TCCCAGAAAG ACAACAGGAT TCAACCTTAC AATCAGGAAG AGATAGTATT
    TTCAGAGAAA GTAAAATATT TAGGAATGCT ACTTCCTTTG AGTACACTGA AATCTCCCCA
    CAGAAAGAGA GGACTCAGGA ACAAACCTAT GAAAAAGAAC TATTTGTGAG TTCTGAGCTG
    CAAGAAGAAG TCTCAACATC ACGAAGAAAA GATCATCTTT CAGAAACTAC AAAAAAGGAA
    ATTCAGAAAG ATCCATCCTG TGAAAAGTCC CAAAAAATAG AAGGAAAGTC ATGGTCAGGT
    GAACTTCTTA CTTGTAACTG GAAGATGAAT TATTTCAAAT GTGAAGATGA GGAGCAAGCC
    AACTTACTCT ACAGTGATAA CAGGTTCACA GACCTACACT CAATTATCAG AAATATTCAG
    TCTTACAAGG AAGTAAAAGG TAGAAGTGCT TTCAATGGAG TTTCCTTCAA TCTGCTCCAG
    TTTGTGCAAC TTTTGGAGAC ATTTGTTGGT GAAGATGTCC CCTTGAGTGT CAGTGAGACT
    CTCACAACCT TTTTTAAGAG GGGCTATGTT GAAACAAAAG AAGAGAAAAT AAGTGGCTTA
    GAACAGACTA GACAAAATGC TTCCAGAGTC CGACAGGAAC TTCTCCTAGA AGCCCTGTTT
    CAGAAGTGGG ACAGTGATGG CTCAGGCTTC TTGGATCTGA AGGAAATTGA TGAACTTTTG
    TACACATACA AGGAGGGAAT GGAAAAAGAA TCCATGAAGA AAGCTAAACT ACACATCCAA
    TTTCCAAAGC CACAACCTGA TCAAGAAGTG AGACTGTCTT CAAAACAATT TCAGAAATAC
    ATAGAATTGG TTGTCTCTGA ACTCAGGGGC AATGAAGATC ATGTTCTGGA AAGTATTGTG
    GAGTTTCTGG TGAATACTTT AGAAAGGAGC CATACTGAGA ATCTGAGGAA TTGTGCCAGA
    CGAAAGTGGC TATACCAAAT CCAACATGCT GCAGAGACAA GTGGAGTATC CCTGGAGCCT
    GTGTACACTG AAACCTTTAA GGCCCTCACC CAGGATGCTG AAGCCCATGG AAATAAGAAG
    ATCTGTGCTC ATATTTCCCT CTTAGAAGAA AACCTACTAC TGCCTGATAG AGGGGAAGTT
    GTATTAAGGA ACGTAGCTTG TACTTTAGAT GATGCTCCAT TTGTACTGAA CAAAGTGCTC
    TACAGGGACA TGAAGGGAAT CAGCTTTACA GTAGTGGATG AAGGTAAGCC AATACACGTC
    CCCCAAGTTC AGCACCATGG GAACGTCTTT TTCTGGAACC ATTCCCTCAA GAAGAGTGAT
    CAAAATGGAT CATTCCTGGC CCTGCCTCTT CAGGATGCAT ACATGAGGAT CTTTGGGGTC
    ATGGCTGTGG ACACCCTAAG AGATAAAAAA AAAATAAACA TCTTTGTACC TCATGAGATC
    AGATTCTATC AGGGTGTTGC TAATGTCTTT AGCACTGCCT ATCACTATGT CCACAGCAGG
    GAGCACATCC TGCATGTTGT GATTACTGGC ATTGGCTGGC TCTGTGACAT CACATCTGAC
    ATCACCGCCA TCACTACATA CTTCATTGAG CCTGGCCTAA TGCAGGATTC AGACTATGTT
    TTACGCAATA TGATGGTTAC CAGACACCTG GGTCTAACAG AAATCCACAG GAACCCCCCT
    ATAATTTTCA GGAAGACATG CATCTTCAGA GACTTCCTCT TTAAATGTAC AGACAGTTCC
    GAAGTTGTCT TGGCCTCTGT CTGTGGAGAA AACCACATAC TAATTCCACT CCGTGAGAGA
    ACAGGAAAAG CTTTGGGAGT TCTTGATTTT AACATTGGCA AGAGTAAAAT GTTATTGTAT
    CGAGAATTTA AAGACCTACA GAAAATGATG AAAGTGGTCC AAGCTGCCTG CTACGAAATA
    CTGGGCGAAT TATCTGGAGA AATAAAGAAA AACTATATCC TAGAGATTGA AAATGTAGGA
    GAAGTCCAGC GGGCAGGAGT TCTCTTTTTC CGAATCATGC TACAAGAGCT GCAAGAAAGC
    CTCCGATTGC TTACCTCCAT AGACTTTGTA TCACTGTTGA TCTATGATTA TCATATTCCA
    GCAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12277
    Clone ID Related Accession (Same CDS sequence) XM_022423407.1 , XM_022423417.1 , XM_005624901.3 , XM_005624899.2 , XM_005624900.2 , XM_005624899.3 , XM_005624901.2
    Accession Version XM_022423407.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279115.1
    CDS188..4681
    Translation

    Target ORF information:

    RefSeq Version XM_022423407.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423407.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12277
    Clone ID Related Accession (Same CDS sequence) XM_022423407.1 , XM_022423417.1 , XM_005624901.3 , XM_005624899.2 , XM_005624900.2 , XM_005624899.3 , XM_005624901.2
    Accession Version XM_022423417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279125.1
    CDS128..4621
    Translation

    Target ORF information:

    RefSeq Version XM_022423417.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12277
    Clone ID Related Accession (Same CDS sequence) XM_022423407.1 , XM_022423417.1 , XM_005624901.3 , XM_005624899.2 , XM_005624900.2 , XM_005624899.3 , XM_005624901.2
    Accession Version XM_005624901.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624958.1
    CDS165..4658
    Translation

    Target ORF information:

    RefSeq Version XM_005624901.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624901.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12277
    Clone ID Related Accession (Same CDS sequence) XM_022423407.1 , XM_022423417.1 , XM_005624901.3 , XM_005624899.2 , XM_005624900.2 , XM_005624899.3 , XM_005624901.2
    Accession Version XM_005624899.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624956.1
    CDS363..4856
    Translation

    Target ORF information:

    RefSeq Version XM_005624899.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624899.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12277
    Clone ID Related Accession (Same CDS sequence) XM_022423407.1 , XM_022423417.1 , XM_005624901.3 , XM_005624899.2 , XM_005624900.2 , XM_005624899.3 , XM_005624901.2
    Accession Version XM_005624900.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624957.1
    CDS96..4589
    Translation

    Target ORF information:

    RefSeq Version XM_005624900.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624900.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12277
    Clone ID Related Accession (Same CDS sequence) XM_022423407.1 , XM_022423417.1 , XM_005624901.3 , XM_005624899.2 , XM_005624900.2 , XM_005624899.3 , XM_005624901.2
    Accession Version XM_005624899.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624899.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624956.1
    CDS220..4713
    Translation

    Target ORF information:

    RefSeq Version XM_005624899.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624899.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12277
    Clone ID Related Accession (Same CDS sequence) XM_022423407.1 , XM_022423417.1 , XM_005624901.3 , XM_005624899.2 , XM_005624900.2 , XM_005624899.3 , XM_005624901.2
    Accession Version XM_005624901.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624958.1
    CDS308..4801
    Translation

    Target ORF information:

    RefSeq Version XM_005624901.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624901.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GTCATTCTCG AATTGAAGTA
    TGGAAATTTG GTAATATTGT CATCGAATAC CTATACCACT GGGCACACAT CTGCTTAGCT
    CTCATGGAGC TAAACAAAAA ACCAAGCAGT GCTATTTTAC CCCCATTGCC CTCCAAAACT
    GACTCCTGTG TTTATGCAAA AATGCCAAAG GGAAACTTGC CAGGGAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12278
    Clone ID Related Accession (Same CDS sequence) XM_005624902.2 , XM_022423410.1 , XM_005624902.3
    Accession Version XM_005624902.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTTTGG AAAATGGAAA AGAAGACAAA GAAAACAAAC CCGAAGAAAC TGAAGTGAAA 
    GAACCTACTG AGAGTTCACA GGGTGTACCA GGCAGTTCTG CATTAGACAC CAGCAGCAAT
    GTGGCCGAAA CAGAAGCAGA TGAAATCAAA TCCCAAGAAA TACACCTGGA AGGGCAGTCA
    AAAACTTCAG TTTGTTCAAT AAAGGACTCT AGCACTAGAG TCTCAAAGAA TTTTGTGGTT
    AGAAAACCTG CAAAAGTGAT CTTCAATTTA GATCCAACTG CGCTGAATTC AAAGCTAGAA
    CAGCCATGGA AGAAAAATTT TTTTGAAAGA TTGGAGGCAA AAGCCCAGAA AATGCAGCAG
    AAAATAATAG ATAAGGATAA CCTGAATAAA GAACTAGAAA AAAAGGCTGA AAAAAACCTC
    CCTAGGGATA ATTTGGCCAA AGAGTGGTTT AATGCTGAAA GCATAACATT GGACAGTCGT
    GCATATTTGC TGGACAAACT TCTACCTACC TTAGTTCCTG GCGTGGAAAA AATGTTAATG
    CAAGTGGAAA AGAAGAAGCT TTGGGCAGAA GGTGATATTA CAACCAAGTT TAATCCAAAT
    AATCATTTGG GGGAATACTT AATGAGAAAC AATCCTTATT ATATAAAAGA TTCAGGAATG
    TCTGGTTATC AAAGAGTGAT GAGGGATGTC ACAGAAGACC TGAAGATACA TGTTCCCAAC
    ACTGTTGGCA ACAGAGTAAC CAAGATGAAG GAGAGTATTA AACAGAAACG AGAACAAAGA
    CAATTCATAA AAGAAGTCAA AGTCAGGGTG ACCAAAACAC GGAAGCAGGC TCTGCAGGAG
    CAATTCAGTG AATGGATTCT AGACCCCAAA GGAACAATTC CTATGGTTGT GATTCAGAAT
    GTTCTTCATG AATTTTTTCA AAATCCAGAT TTACAGCTTG AAATATGTTG CAAACCAATG
    GATATTGTTG ACTCGATGGG ACCAAGATTG AACAAAACAG AATTTATAGA ATATGTCTCT
    TCATATGTTG CAGACTTTAA AAATGAAATG TTCGAGAAAC TCCTTAAGCA CTTTTGTCAT
    TGTGCAGATG AATTCCGGGA GGTTATAAAA ACAGACATGC GGAGGCAGAT ATTTGCTGAA
    CTCTTCCTGC ATTGTGACCA TGGGAAGGTA GGATTTTTGG ATCGGCAGAG GACATTGGCT
    TTGTTGGAAA CATTCTATGA CCAAAGTTCA AAAATGCTTA GAAGTTTGCT ACGAAACCCA
    AGACAATGGC CATTCATTGA ATTTGAAGAG ATAGATTTGC CTGAGTTCTG GGGAGACATG
    GATAATCAGA AACATATTTA TGAAGACTTT GACAAAGTGC TCTTAGAGAT GAATGCACAA
    CTTTCTGAAA AATATGCTAG CAAAACCCAA AGCAAATTGT TAGAAACTCC AGAAGATCAA
    CATTATCATG ATGAACATAG TGAATCAACA CTACCAGAAC AGCAGAGAGG GACAACTGCA
    GAACAAGAAC GAAACAGAAT TTTAAGCAAG GAACAAGAAC AAGGCAGAGA GTTAACTGGA
    GAAAAAGAAC TAGACAAAGA CTCAGTAACA GAACAAGAAA CCCACACAGG GTCAACTCCA
    GAACAGGGAT CAAGTAGAGA GTTAATAATA GAACAAAGAC TACATAGTGA CTCAGTAATA
    GAACAAGAGC CACCACAAAG CATGTCTTCT TCAGAACAAG GAGTCAACAT GGGAACAACT
    GCAGAACAAG GACTCTACAG AGAATCAATA GCAGCACAAG GACAACACAA ACGGTCAGAC
    ACAGAACAAG GACCACACAG AGGGTCAACC TCAGAACAAG GATCACGCAG ACAGTCAGTA
    ATAGAACAAG AACCACTCAA AGGGTCAACT GCAGAACAAG AGCCATCCAG AGAGATATTC
    CCAGAAAGAC AACAGGATTC AACCTTACAA TCAGGAAGAG ATAGTATTTT CAGAGAAAGT
    AAAATATTTA GGAATGCTAC TTCCTTTGAG TACACTGAAA TCTCCCCACA GAAAGAGAGG
    ACTCAGGAAC AAACCTATGA AAAAGAACTA TTTGTGAGTT CTGAGCTGCA AGAAGAAGTC
    TCAACATCAC GAAGAAAAGA TCATCTTTCA GAAACTACAA AAAAGGAAAT TCAGAAAGAT
    CCATCCTGTG AAAAGTCCCA AAAAATAGAA GGAAAGTCAT GGTCAGGTGA ACTTCTTACT
    TGTAACTGGA AGATGAATTA TTTCAAATGT GAAGATGAGG AGCAAGCCAA CTTACTCTAC
    AGTGATAACA GGTTCACAGA CCTACACTCA ATTATCAGAA ATATTCAGTC TTACAAGGAA
    GTAAAAGGTA GAAGTGCTTT CAATGGAGTT TCCTTCAATC TGCTCCAGTT TGTGCAACTT
    TTGGAGACAT TTGTTGGTGA AGATGTCCCC TTGAGTGTCA GTGAGACTCT CACAACCTTT
    TTTAAGAGGG GCTATGTTGA AACAAAAGAA GAGAAAATAA GTGGCTTAGA ACAGACTAGA
    CAAAATGCTT CCAGAGTCCG ACAGGAACTT CTCCTAGAAG CCCTGTTTCA GAAGTGGGAC
    AGTGATGGCT CAGGCTTCTT GGATCTGAAG GAAATTGATG AACTTTTGTA CACATACAAG
    GAGGGAATGG AAAAAGAATC CATGAAGAAA GCTAAACTAC ACATCCAATT TCCAAAGCCA
    CAACCTGATC AAGAAGTGAG ACTGTCTTCA AAACAATTTC AGAAATACAT AGAATTGGTT
    GTCTCTGAAC TCAGGGGCAA TGAAGATCAT GTTCTGGAAA GTATTGTGGA GTTTCTGGTG
    AATACTTTAG AAAGGAGCCA TACTGAGAAT CTGAGGAATT GTGCCAGACG AAAGTGGCTA
    TACCAAATCC AACATGCTGC AGAGACAAGT GGAGTATCCC TGGAGCCTGT GTACACTGAA
    ACCTTTAAGG CCCTCACCCA GGATGCTGAA GCCCATGGAA ATAAGAAGAT CTGTGCTCAT
    ATTTCCCTCT TAGAAGAAAA CCTACTACTG CCTGATAGAG GGGAAGTTGT ATTAAGGAAC
    GTAGCTTGTA CTTTAGATGA TGCTCCATTT GTACTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGTAAGCCAA TACACGTCCC CCAAGTTCAG
    CACCATGGGA ACGTCTTTTT CTGGAACCAT TCCCTCAAGA AGAGTGATCA AAATGGATCA
    TTCCTGGCCC TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCAT GGCTGTGGAC
    ACCCTAAGAG ATAAAAAAAA AATAAACATC TTTGTACCTC ATGAGATCAG ATTCTATCAG
    GGTGTTGCTA ATGTCTTTAG CACTGCCTAT CACTATGTCC ACAGCAGGGA GCACATCCTG
    CATGTTGTGA TTACTGGCAT TGGCTGGCTC TGTGACATCA CATCTGACAT CACCGCCATC
    ACTACATACT TCATTGAGCC TGGCCTAATG CAGGCTTCTT CCTCTCTGAG TGGTTGGACC
    AAGCTGATAA GGCCCTGGCC AAAGAGCAGA ATCACAGAGC TCTCTGGCAG AATGAACAGA
    CTGTGGAGGT GGAGAACGCA TAGTCCCGGA GACTTCCTCT TTAAATGTAC AGACAGTTCC
    GAAGTTGTCT TGGCCTCTGT CTGTGGAGAA AACCACATAC TAATTCCACT CCGTGAGAGA
    ACAGGAAAAG CTTTGGGAGT TCTTGATTTT AACATTGGCA AGAGTAAAAT GTTATTGTAT
    CGAGAATTTA AAGACCTACA GAAAATGATG AAAGTGGTCC AAGCTGCCTG CTACGAAATA
    CTGGGCGAAT TATCTGGAGA AATAAAGAAA AACTATATCC TAGAGATTGA AAATGTAGGA
    GAAGTCCAGC GGGCAGGAGT TCTCTTTTTC CGAATCATGC TACAAGAGCT GCAAGAAAGC
    CTCCGATTGC TTACCTCCAT AGACTTTGTA TCACTGTTGA TCTATGATTA TCATATTCCA
    GCAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624959.1
    CDS346..4806
    Translation

    Target ORF information:

    RefSeq Version XM_005624902.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624902.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTTTGG AAAATGGAAA AGAAGACAAA GAAAACAAAC CCGAAGAAAC TGAAGTGAAA 
    GAACCTACTG AGAGTTCACA GGGTGTACCA GGCAGTTCTG CATTAGACAC CAGCAGCAAT
    GTGGCCGAAA CAGAAGCAGA TGAAATCAAA TCCCAAGAAA TACACCTGGA AGGGCAGTCA
    AAAACTTCAG TTTGTTCAAT AAAGGACTCT AGCACTAGAG TCTCAAAGAA TTTTGTGGTT
    AGAAAACCTG CAAAAGTGAT CTTCAATTTA GATCCAACTG CGCTGAATTC AAAGCTAGAA
    CAGCCATGGA AGAAAAATTT TTTTGAAAGA TTGGAGGCAA AAGCCCAGAA AATGCAGCAG
    AAAATAATAG ATAAGGATAA CCTGAATAAA GAACTAGAAA AAAAGGCTGA AAAAAACCTC
    CCTAGGGATA ATTTGGCCAA AGAGTGGTTT AATGCTGAAA GCATAACATT GGACAGTCGT
    GCATATTTGC TGGACAAACT TCTACCTACC TTAGTTCCTG GCGTGGAAAA AATGTTAATG
    CAAGTGGAAA AGAAGAAGCT TTGGGCAGAA GGTGATATTA CAACCAAGTT TAATCCAAAT
    AATCATTTGG GGGAATACTT AATGAGAAAC AATCCTTATT ATATAAAAGA TTCAGGAATG
    TCTGGTTATC AAAGAGTGAT GAGGGATGTC ACAGAAGACC TGAAGATACA TGTTCCCAAC
    ACTGTTGGCA ACAGAGTAAC CAAGATGAAG GAGAGTATTA AACAGAAACG AGAACAAAGA
    CAATTCATAA AAGAAGTCAA AGTCAGGGTG ACCAAAACAC GGAAGCAGGC TCTGCAGGAG
    CAATTCAGTG AATGGATTCT AGACCCCAAA GGAACAATTC CTATGGTTGT GATTCAGAAT
    GTTCTTCATG AATTTTTTCA AAATCCAGAT TTACAGCTTG AAATATGTTG CAAACCAATG
    GATATTGTTG ACTCGATGGG ACCAAGATTG AACAAAACAG AATTTATAGA ATATGTCTCT
    TCATATGTTG CAGACTTTAA AAATGAAATG TTCGAGAAAC TCCTTAAGCA CTTTTGTCAT
    TGTGCAGATG AATTCCGGGA GGTTATAAAA ACAGACATGC GGAGGCAGAT ATTTGCTGAA
    CTCTTCCTGC ATTGTGACCA TGGGAAGGTA GGATTTTTGG ATCGGCAGAG GACATTGGCT
    TTGTTGGAAA CATTCTATGA CCAAAGTTCA AAAATGCTTA GAAGTTTGCT ACGAAACCCA
    AGACAATGGC CATTCATTGA ATTTGAAGAG ATAGATTTGC CTGAGTTCTG GGGAGACATG
    GATAATCAGA AACATATTTA TGAAGACTTT GACAAAGTGC TCTTAGAGAT GAATGCACAA
    CTTTCTGAAA AATATGCTAG CAAAACCCAA AGCAAATTGT TAGAAACTCC AGAAGATCAA
    CATTATCATG ATGAACATAG TGAATCAACA CTACCAGAAC AGCAGAGAGG GACAACTGCA
    GAACAAGAAC GAAACAGAAT TTTAAGCAAG GAACAAGAAC AAGGCAGAGA GTTAACTGGA
    GAAAAAGAAC TAGACAAAGA CTCAGTAACA GAACAAGAAA CCCACACAGG GTCAACTCCA
    GAACAGGGAT CAAGTAGAGA GTTAATAATA GAACAAAGAC TACATAGTGA CTCAGTAATA
    GAACAAGAGC CACCACAAAG CATGTCTTCT TCAGAACAAG GAGTCAACAT GGGAACAACT
    GCAGAACAAG GACTCTACAG AGAATCAATA GCAGCACAAG GACAACACAA ACGGTCAGAC
    ACAGAACAAG GACCACACAG AGGGTCAACC TCAGAACAAG GATCACGCAG ACAGTCAGTA
    ATAGAACAAG AACCACTCAA AGGGTCAACT GCAGAACAAG AGCCATCCAG AGAGATATTC
    CCAGAAAGAC AACAGGATTC AACCTTACAA TCAGGAAGAG ATAGTATTTT CAGAGAAAGT
    AAAATATTTA GGAATGCTAC TTCCTTTGAG TACACTGAAA TCTCCCCACA GAAAGAGAGG
    ACTCAGGAAC AAACCTATGA AAAAGAACTA TTTGTGAGTT CTGAGCTGCA AGAAGAAGTC
    TCAACATCAC GAAGAAAAGA TCATCTTTCA GAAACTACAA AAAAGGAAAT TCAGAAAGAT
    CCATCCTGTG AAAAGTCCCA AAAAATAGAA GGAAAGTCAT GGTCAGGTGA ACTTCTTACT
    TGTAACTGGA AGATGAATTA TTTCAAATGT GAAGATGAGG AGCAAGCCAA CTTACTCTAC
    AGTGATAACA GGTTCACAGA CCTACACTCA ATTATCAGAA ATATTCAGTC TTACAAGGAA
    GTAAAAGGTA GAAGTGCTTT CAATGGAGTT TCCTTCAATC TGCTCCAGTT TGTGCAACTT
    TTGGAGACAT TTGTTGGTGA AGATGTCCCC TTGAGTGTCA GTGAGACTCT CACAACCTTT
    TTTAAGAGGG GCTATGTTGA AACAAAAGAA GAGAAAATAA GTGGCTTAGA ACAGACTAGA
    CAAAATGCTT CCAGAGTCCG ACAGGAACTT CTCCTAGAAG CCCTGTTTCA GAAGTGGGAC
    AGTGATGGCT CAGGCTTCTT GGATCTGAAG GAAATTGATG AACTTTTGTA CACATACAAG
    GAGGGAATGG AAAAAGAATC CATGAAGAAA GCTAAACTAC ACATCCAATT TCCAAAGCCA
    CAACCTGATC AAGAAGTGAG ACTGTCTTCA AAACAATTTC AGAAATACAT AGAATTGGTT
    GTCTCTGAAC TCAGGGGCAA TGAAGATCAT GTTCTGGAAA GTATTGTGGA GTTTCTGGTG
    AATACTTTAG AAAGGAGCCA TACTGAGAAT CTGAGGAATT GTGCCAGACG AAAGTGGCTA
    TACCAAATCC AACATGCTGC AGAGACAAGT GGAGTATCCC TGGAGCCTGT GTACACTGAA
    ACCTTTAAGG CCCTCACCCA GGATGCTGAA GCCCATGGAA ATAAGAAGAT CTGTGCTCAT
    ATTTCCCTCT TAGAAGAAAA CCTACTACTG CCTGATAGAG GGGAAGTTGT ATTAAGGAAC
    GTAGCTTGTA CTTTAGATGA TGCTCCATTT GTACTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGTAAGCCAA TACACGTCCC CCAAGTTCAG
    CACCATGGGA ACGTCTTTTT CTGGAACCAT TCCCTCAAGA AGAGTGATCA AAATGGATCA
    TTCCTGGCCC TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCAT GGCTGTGGAC
    ACCCTAAGAG ATAAAAAAAA AATAAACATC TTTGTACCTC ATGAGATCAG ATTCTATCAG
    GGTGTTGCTA ATGTCTTTAG CACTGCCTAT CACTATGTCC ACAGCAGGGA GCACATCCTG
    CATGTTGTGA TTACTGGCAT TGGCTGGCTC TGTGACATCA CATCTGACAT CACCGCCATC
    ACTACATACT TCATTGAGCC TGGCCTAATG CAGGCTTCTT CCTCTCTGAG TGGTTGGACC
    AAGCTGATAA GGCCCTGGCC AAAGAGCAGA ATCACAGAGC TCTCTGGCAG AATGAACAGA
    CTGTGGAGGT GGAGAACGCA TAGTCCCGGA GACTTCCTCT TTAAATGTAC AGACAGTTCC
    GAAGTTGTCT TGGCCTCTGT CTGTGGAGAA AACCACATAC TAATTCCACT CCGTGAGAGA
    ACAGGAAAAG CTTTGGGAGT TCTTGATTTT AACATTGGCA AGAGTAAAAT GTTATTGTAT
    CGAGAATTTA AAGACCTACA GAAAATGATG AAAGTGGTCC AAGCTGCCTG CTACGAAATA
    CTGGGCGAAT TATCTGGAGA AATAAAGAAA AACTATATCC TAGAGATTGA AAATGTAGGA
    GAAGTCCAGC GGGCAGGAGT TCTCTTTTTC CGAATCATGC TACAAGAGCT GCAAGAAAGC
    CTCCGATTGC TTACCTCCAT AGACTTTGTA TCACTGTTGA TCTATGATTA TCATATTCCA
    GCAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12278
    Clone ID Related Accession (Same CDS sequence) XM_005624902.2 , XM_022423410.1 , XM_005624902.3
    Accession Version XM_022423410.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTTTGG AAAATGGAAA AGAAGACAAA GAAAACAAAC CCGAAGAAAC TGAAGTGAAA 
    GAACCTACTG AGAGTTCACA GGGTGTACCA GGCAGTTCTG CATTAGACAC CAGCAGCAAT
    GTGGCCGAAA CAGAAGCAGA TGAAATCAAA TCCCAAGAAA TACACCTGGA AGGGCAGTCA
    AAAACTTCAG TTTGTTCAAT AAAGGACTCT AGCACTAGAG TCTCAAAGAA TTTTGTGGTT
    AGAAAACCTG CAAAAGTGAT CTTCAATTTA GATCCAACTG CGCTGAATTC AAAGCTAGAA
    CAGCCATGGA AGAAAAATTT TTTTGAAAGA TTGGAGGCAA AAGCCCAGAA AATGCAGCAG
    AAAATAATAG ATAAGGATAA CCTGAATAAA GAACTAGAAA AAAAGGCTGA AAAAAACCTC
    CCTAGGGATA ATTTGGCCAA AGAGTGGTTT AATGCTGAAA GCATAACATT GGACAGTCGT
    GCATATTTGC TGGACAAACT TCTACCTACC TTAGTTCCTG GCGTGGAAAA AATGTTAATG
    CAAGTGGAAA AGAAGAAGCT TTGGGCAGAA GGTGATATTA CAACCAAGTT TAATCCAAAT
    AATCATTTGG GGGAATACTT AATGAGAAAC AATCCTTATT ATATAAAAGA TTCAGGAATG
    TCTGGTTATC AAAGAGTGAT GAGGGATGTC ACAGAAGACC TGAAGATACA TGTTCCCAAC
    ACTGTTGGCA ACAGAGTAAC CAAGATGAAG GAGAGTATTA AACAGAAACG AGAACAAAGA
    CAATTCATAA AAGAAGTCAA AGTCAGGGTG ACCAAAACAC GGAAGCAGGC TCTGCAGGAG
    CAATTCAGTG AATGGATTCT AGACCCCAAA GGAACAATTC CTATGGTTGT GATTCAGAAT
    GTTCTTCATG AATTTTTTCA AAATCCAGAT TTACAGCTTG AAATATGTTG CAAACCAATG
    GATATTGTTG ACTCGATGGG ACCAAGATTG AACAAAACAG AATTTATAGA ATATGTCTCT
    TCATATGTTG CAGACTTTAA AAATGAAATG TTCGAGAAAC TCCTTAAGCA CTTTTGTCAT
    TGTGCAGATG AATTCCGGGA GGTTATAAAA ACAGACATGC GGAGGCAGAT ATTTGCTGAA
    CTCTTCCTGC ATTGTGACCA TGGGAAGGTA GGATTTTTGG ATCGGCAGAG GACATTGGCT
    TTGTTGGAAA CATTCTATGA CCAAAGTTCA AAAATGCTTA GAAGTTTGCT ACGAAACCCA
    AGACAATGGC CATTCATTGA ATTTGAAGAG ATAGATTTGC CTGAGTTCTG GGGAGACATG
    GATAATCAGA AACATATTTA TGAAGACTTT GACAAAGTGC TCTTAGAGAT GAATGCACAA
    CTTTCTGAAA AATATGCTAG CAAAACCCAA AGCAAATTGT TAGAAACTCC AGAAGATCAA
    CATTATCATG ATGAACATAG TGAATCAACA CTACCAGAAC AGCAGAGAGG GACAACTGCA
    GAACAAGAAC GAAACAGAAT TTTAAGCAAG GAACAAGAAC AAGGCAGAGA GTTAACTGGA
    GAAAAAGAAC TAGACAAAGA CTCAGTAACA GAACAAGAAA CCCACACAGG GTCAACTCCA
    GAACAGGGAT CAAGTAGAGA GTTAATAATA GAACAAAGAC TACATAGTGA CTCAGTAATA
    GAACAAGAGC CACCACAAAG CATGTCTTCT TCAGAACAAG GAGTCAACAT GGGAACAACT
    GCAGAACAAG GACTCTACAG AGAATCAATA GCAGCACAAG GACAACACAA ACGGTCAGAC
    ACAGAACAAG GACCACACAG AGGGTCAACC TCAGAACAAG GATCACGCAG ACAGTCAGTA
    ATAGAACAAG AACCACTCAA AGGGTCAACT GCAGAACAAG AGCCATCCAG AGAGATATTC
    CCAGAAAGAC AACAGGATTC AACCTTACAA TCAGGAAGAG ATAGTATTTT CAGAGAAAGT
    AAAATATTTA GGAATGCTAC TTCCTTTGAG TACACTGAAA TCTCCCCACA GAAAGAGAGG
    ACTCAGGAAC AAACCTATGA AAAAGAACTA TTTGTGAGTT CTGAGCTGCA AGAAGAAGTC
    TCAACATCAC GAAGAAAAGA TCATCTTTCA GAAACTACAA AAAAGGAAAT TCAGAAAGAT
    CCATCCTGTG AAAAGTCCCA AAAAATAGAA GGAAAGTCAT GGTCAGGTGA ACTTCTTACT
    TGTAACTGGA AGATGAATTA TTTCAAATGT GAAGATGAGG AGCAAGCCAA CTTACTCTAC
    AGTGATAACA GGTTCACAGA CCTACACTCA ATTATCAGAA ATATTCAGTC TTACAAGGAA
    GTAAAAGGTA GAAGTGCTTT CAATGGAGTT TCCTTCAATC TGCTCCAGTT TGTGCAACTT
    TTGGAGACAT TTGTTGGTGA AGATGTCCCC TTGAGTGTCA GTGAGACTCT CACAACCTTT
    TTTAAGAGGG GCTATGTTGA AACAAAAGAA GAGAAAATAA GTGGCTTAGA ACAGACTAGA
    CAAAATGCTT CCAGAGTCCG ACAGGAACTT CTCCTAGAAG CCCTGTTTCA GAAGTGGGAC
    AGTGATGGCT CAGGCTTCTT GGATCTGAAG GAAATTGATG AACTTTTGTA CACATACAAG
    GAGGGAATGG AAAAAGAATC CATGAAGAAA GCTAAACTAC ACATCCAATT TCCAAAGCCA
    CAACCTGATC AAGAAGTGAG ACTGTCTTCA AAACAATTTC AGAAATACAT AGAATTGGTT
    GTCTCTGAAC TCAGGGGCAA TGAAGATCAT GTTCTGGAAA GTATTGTGGA GTTTCTGGTG
    AATACTTTAG AAAGGAGCCA TACTGAGAAT CTGAGGAATT GTGCCAGACG AAAGTGGCTA
    TACCAAATCC AACATGCTGC AGAGACAAGT GGAGTATCCC TGGAGCCTGT GTACACTGAA
    ACCTTTAAGG CCCTCACCCA GGATGCTGAA GCCCATGGAA ATAAGAAGAT CTGTGCTCAT
    ATTTCCCTCT TAGAAGAAAA CCTACTACTG CCTGATAGAG GGGAAGTTGT ATTAAGGAAC
    GTAGCTTGTA CTTTAGATGA TGCTCCATTT GTACTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGTAAGCCAA TACACGTCCC CCAAGTTCAG
    CACCATGGGA ACGTCTTTTT CTGGAACCAT TCCCTCAAGA AGAGTGATCA AAATGGATCA
    TTCCTGGCCC TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCAT GGCTGTGGAC
    ACCCTAAGAG ATAAAAAAAA AATAAACATC TTTGTACCTC ATGAGATCAG ATTCTATCAG
    GGTGTTGCTA ATGTCTTTAG CACTGCCTAT CACTATGTCC ACAGCAGGGA GCACATCCTG
    CATGTTGTGA TTACTGGCAT TGGCTGGCTC TGTGACATCA CATCTGACAT CACCGCCATC
    ACTACATACT TCATTGAGCC TGGCCTAATG CAGGCTTCTT CCTCTCTGAG TGGTTGGACC
    AAGCTGATAA GGCCCTGGCC AAAGAGCAGA ATCACAGAGC TCTCTGGCAG AATGAACAGA
    CTGTGGAGGT GGAGAACGCA TAGTCCCGGA GACTTCCTCT TTAAATGTAC AGACAGTTCC
    GAAGTTGTCT TGGCCTCTGT CTGTGGAGAA AACCACATAC TAATTCCACT CCGTGAGAGA
    ACAGGAAAAG CTTTGGGAGT TCTTGATTTT AACATTGGCA AGAGTAAAAT GTTATTGTAT
    CGAGAATTTA AAGACCTACA GAAAATGATG AAAGTGGTCC AAGCTGCCTG CTACGAAATA
    CTGGGCGAAT TATCTGGAGA AATAAAGAAA AACTATATCC TAGAGATTGA AAATGTAGGA
    GAAGTCCAGC GGGCAGGAGT TCTCTTTTTC CGAATCATGC TACAAGAGCT GCAAGAAAGC
    CTCCGATTGC TTACCTCCAT AGACTTTGTA TCACTGTTGA TCTATGATTA TCATATTCCA
    GCAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279118.1
    CDS171..4631
    Translation

    Target ORF information:

    RefSeq Version XM_022423410.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423410.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTTTGG AAAATGGAAA AGAAGACAAA GAAAACAAAC CCGAAGAAAC TGAAGTGAAA 
    GAACCTACTG AGAGTTCACA GGGTGTACCA GGCAGTTCTG CATTAGACAC CAGCAGCAAT
    GTGGCCGAAA CAGAAGCAGA TGAAATCAAA TCCCAAGAAA TACACCTGGA AGGGCAGTCA
    AAAACTTCAG TTTGTTCAAT AAAGGACTCT AGCACTAGAG TCTCAAAGAA TTTTGTGGTT
    AGAAAACCTG CAAAAGTGAT CTTCAATTTA GATCCAACTG CGCTGAATTC AAAGCTAGAA
    CAGCCATGGA AGAAAAATTT TTTTGAAAGA TTGGAGGCAA AAGCCCAGAA AATGCAGCAG
    AAAATAATAG ATAAGGATAA CCTGAATAAA GAACTAGAAA AAAAGGCTGA AAAAAACCTC
    CCTAGGGATA ATTTGGCCAA AGAGTGGTTT AATGCTGAAA GCATAACATT GGACAGTCGT
    GCATATTTGC TGGACAAACT TCTACCTACC TTAGTTCCTG GCGTGGAAAA AATGTTAATG
    CAAGTGGAAA AGAAGAAGCT TTGGGCAGAA GGTGATATTA CAACCAAGTT TAATCCAAAT
    AATCATTTGG GGGAATACTT AATGAGAAAC AATCCTTATT ATATAAAAGA TTCAGGAATG
    TCTGGTTATC AAAGAGTGAT GAGGGATGTC ACAGAAGACC TGAAGATACA TGTTCCCAAC
    ACTGTTGGCA ACAGAGTAAC CAAGATGAAG GAGAGTATTA AACAGAAACG AGAACAAAGA
    CAATTCATAA AAGAAGTCAA AGTCAGGGTG ACCAAAACAC GGAAGCAGGC TCTGCAGGAG
    CAATTCAGTG AATGGATTCT AGACCCCAAA GGAACAATTC CTATGGTTGT GATTCAGAAT
    GTTCTTCATG AATTTTTTCA AAATCCAGAT TTACAGCTTG AAATATGTTG CAAACCAATG
    GATATTGTTG ACTCGATGGG ACCAAGATTG AACAAAACAG AATTTATAGA ATATGTCTCT
    TCATATGTTG CAGACTTTAA AAATGAAATG TTCGAGAAAC TCCTTAAGCA CTTTTGTCAT
    TGTGCAGATG AATTCCGGGA GGTTATAAAA ACAGACATGC GGAGGCAGAT ATTTGCTGAA
    CTCTTCCTGC ATTGTGACCA TGGGAAGGTA GGATTTTTGG ATCGGCAGAG GACATTGGCT
    TTGTTGGAAA CATTCTATGA CCAAAGTTCA AAAATGCTTA GAAGTTTGCT ACGAAACCCA
    AGACAATGGC CATTCATTGA ATTTGAAGAG ATAGATTTGC CTGAGTTCTG GGGAGACATG
    GATAATCAGA AACATATTTA TGAAGACTTT GACAAAGTGC TCTTAGAGAT GAATGCACAA
    CTTTCTGAAA AATATGCTAG CAAAACCCAA AGCAAATTGT TAGAAACTCC AGAAGATCAA
    CATTATCATG ATGAACATAG TGAATCAACA CTACCAGAAC AGCAGAGAGG GACAACTGCA
    GAACAAGAAC GAAACAGAAT TTTAAGCAAG GAACAAGAAC AAGGCAGAGA GTTAACTGGA
    GAAAAAGAAC TAGACAAAGA CTCAGTAACA GAACAAGAAA CCCACACAGG GTCAACTCCA
    GAACAGGGAT CAAGTAGAGA GTTAATAATA GAACAAAGAC TACATAGTGA CTCAGTAATA
    GAACAAGAGC CACCACAAAG CATGTCTTCT TCAGAACAAG GAGTCAACAT GGGAACAACT
    GCAGAACAAG GACTCTACAG AGAATCAATA GCAGCACAAG GACAACACAA ACGGTCAGAC
    ACAGAACAAG GACCACACAG AGGGTCAACC TCAGAACAAG GATCACGCAG ACAGTCAGTA
    ATAGAACAAG AACCACTCAA AGGGTCAACT GCAGAACAAG AGCCATCCAG AGAGATATTC
    CCAGAAAGAC AACAGGATTC AACCTTACAA TCAGGAAGAG ATAGTATTTT CAGAGAAAGT
    AAAATATTTA GGAATGCTAC TTCCTTTGAG TACACTGAAA TCTCCCCACA GAAAGAGAGG
    ACTCAGGAAC AAACCTATGA AAAAGAACTA TTTGTGAGTT CTGAGCTGCA AGAAGAAGTC
    TCAACATCAC GAAGAAAAGA TCATCTTTCA GAAACTACAA AAAAGGAAAT TCAGAAAGAT
    CCATCCTGTG AAAAGTCCCA AAAAATAGAA GGAAAGTCAT GGTCAGGTGA ACTTCTTACT
    TGTAACTGGA AGATGAATTA TTTCAAATGT GAAGATGAGG AGCAAGCCAA CTTACTCTAC
    AGTGATAACA GGTTCACAGA CCTACACTCA ATTATCAGAA ATATTCAGTC TTACAAGGAA
    GTAAAAGGTA GAAGTGCTTT CAATGGAGTT TCCTTCAATC TGCTCCAGTT TGTGCAACTT
    TTGGAGACAT TTGTTGGTGA AGATGTCCCC TTGAGTGTCA GTGAGACTCT CACAACCTTT
    TTTAAGAGGG GCTATGTTGA AACAAAAGAA GAGAAAATAA GTGGCTTAGA ACAGACTAGA
    CAAAATGCTT CCAGAGTCCG ACAGGAACTT CTCCTAGAAG CCCTGTTTCA GAAGTGGGAC
    AGTGATGGCT CAGGCTTCTT GGATCTGAAG GAAATTGATG AACTTTTGTA CACATACAAG
    GAGGGAATGG AAAAAGAATC CATGAAGAAA GCTAAACTAC ACATCCAATT TCCAAAGCCA
    CAACCTGATC AAGAAGTGAG ACTGTCTTCA AAACAATTTC AGAAATACAT AGAATTGGTT
    GTCTCTGAAC TCAGGGGCAA TGAAGATCAT GTTCTGGAAA GTATTGTGGA GTTTCTGGTG
    AATACTTTAG AAAGGAGCCA TACTGAGAAT CTGAGGAATT GTGCCAGACG AAAGTGGCTA
    TACCAAATCC AACATGCTGC AGAGACAAGT GGAGTATCCC TGGAGCCTGT GTACACTGAA
    ACCTTTAAGG CCCTCACCCA GGATGCTGAA GCCCATGGAA ATAAGAAGAT CTGTGCTCAT
    ATTTCCCTCT TAGAAGAAAA CCTACTACTG CCTGATAGAG GGGAAGTTGT ATTAAGGAAC
    GTAGCTTGTA CTTTAGATGA TGCTCCATTT GTACTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGTAAGCCAA TACACGTCCC CCAAGTTCAG
    CACCATGGGA ACGTCTTTTT CTGGAACCAT TCCCTCAAGA AGAGTGATCA AAATGGATCA
    TTCCTGGCCC TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCAT GGCTGTGGAC
    ACCCTAAGAG ATAAAAAAAA AATAAACATC TTTGTACCTC ATGAGATCAG ATTCTATCAG
    GGTGTTGCTA ATGTCTTTAG CACTGCCTAT CACTATGTCC ACAGCAGGGA GCACATCCTG
    CATGTTGTGA TTACTGGCAT TGGCTGGCTC TGTGACATCA CATCTGACAT CACCGCCATC
    ACTACATACT TCATTGAGCC TGGCCTAATG CAGGCTTCTT CCTCTCTGAG TGGTTGGACC
    AAGCTGATAA GGCCCTGGCC AAAGAGCAGA ATCACAGAGC TCTCTGGCAG AATGAACAGA
    CTGTGGAGGT GGAGAACGCA TAGTCCCGGA GACTTCCTCT TTAAATGTAC AGACAGTTCC
    GAAGTTGTCT TGGCCTCTGT CTGTGGAGAA AACCACATAC TAATTCCACT CCGTGAGAGA
    ACAGGAAAAG CTTTGGGAGT TCTTGATTTT AACATTGGCA AGAGTAAAAT GTTATTGTAT
    CGAGAATTTA AAGACCTACA GAAAATGATG AAAGTGGTCC AAGCTGCCTG CTACGAAATA
    CTGGGCGAAT TATCTGGAGA AATAAAGAAA AACTATATCC TAGAGATTGA AAATGTAGGA
    GAAGTCCAGC GGGCAGGAGT TCTCTTTTTC CGAATCATGC TACAAGAGCT GCAAGAAAGC
    CTCCGATTGC TTACCTCCAT AGACTTTGTA TCACTGTTGA TCTATGATTA TCATATTCCA
    GCAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12278
    Clone ID Related Accession (Same CDS sequence) XM_005624902.2 , XM_022423410.1 , XM_005624902.3
    Accession Version XM_005624902.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTTTGG AAAATGGAAA AGAAGACAAA GAAAACAAAC CCGAAGAAAC TGAAGTGAAA 
    GAACCTACTG AGAGTTCACA GGGTGTACCA GGCAGTTCTG CATTAGACAC CAGCAGCAAT
    GTGGCCGAAA CAGAAGCAGA TGAAATCAAA TCCCAAGAAA TACACCTGGA AGGGCAGTCA
    AAAACTTCAG TTTGTTCAAT AAAGGACTCT AGCACTAGAG TCTCAAAGAA TTTTGTGGTT
    AGAAAACCTG CAAAAGTGAT CTTCAATTTA GATCCAACTG CGCTGAATTC AAAGCTAGAA
    CAGCCATGGA AGAAAAATTT TTTTGAAAGA TTGGAGGCAA AAGCCCAGAA AATGCAGCAG
    AAAATAATAG ATAAGGATAA CCTGAATAAA GAACTAGAAA AAAAGGCTGA AAAAAACCTC
    CCTAGGGATA ATTTGGCCAA AGAGTGGTTT AATGCTGAAA GCATAACATT GGACAGTCGT
    GCATATTTGC TGGACAAACT TCTACCTACC TTAGTTCCTG GCGTGGAAAA AATGTTAATG
    CAAGTGGAAA AGAAGAAGCT TTGGGCAGAA GGTGATATTA CAACCAAGTT TAATCCAAAT
    AATCATTTGG GGGAATACTT AATGAGAAAC AATCCTTATT ATATAAAAGA TTCAGGAATG
    TCTGGTTATC AAAGAGTGAT GAGGGATGTC ACAGAAGACC TGAAGATACA TGTTCCCAAC
    ACTGTTGGCA ACAGAGTAAC CAAGATGAAG GAGAGTATTA AACAGAAACG AGAACAAAGA
    CAATTCATAA AAGAAGTCAA AGTCAGGGTG ACCAAAACAC GGAAGCAGGC TCTGCAGGAG
    CAATTCAGTG AATGGATTCT AGACCCCAAA GGAACAATTC CTATGGTTGT GATTCAGAAT
    GTTCTTCATG AATTTTTTCA AAATCCAGAT TTACAGCTTG AAATATGTTG CAAACCAATG
    GATATTGTTG ACTCGATGGG ACCAAGATTG AACAAAACAG AATTTATAGA ATATGTCTCT
    TCATATGTTG CAGACTTTAA AAATGAAATG TTCGAGAAAC TCCTTAAGCA CTTTTGTCAT
    TGTGCAGATG AATTCCGGGA GGTTATAAAA ACAGACATGC GGAGGCAGAT ATTTGCTGAA
    CTCTTCCTGC ATTGTGACCA TGGGAAGGTA GGATTTTTGG ATCGGCAGAG GACATTGGCT
    TTGTTGGAAA CATTCTATGA CCAAAGTTCA AAAATGCTTA GAAGTTTGCT ACGAAACCCA
    AGACAATGGC CATTCATTGA ATTTGAAGAG ATAGATTTGC CTGAGTTCTG GGGAGACATG
    GATAATCAGA AACATATTTA TGAAGACTTT GACAAAGTGC TCTTAGAGAT GAATGCACAA
    CTTTCTGAAA AATATGCTAG CAAAACCCAA AGCAAATTGT TAGAAACTCC AGAAGATCAA
    CATTATCATG ATGAACATAG TGAATCAACA CTACCAGAAC AGCAGAGAGG GACAACTGCA
    GAACAAGAAC GAAACAGAAT TTTAAGCAAG GAACAAGAAC AAGGCAGAGA GTTAACTGGA
    GAAAAAGAAC TAGACAAAGA CTCAGTAACA GAACAAGAAA CCCACACAGG GTCAACTCCA
    GAACAGGGAT CAAGTAGAGA GTTAATAATA GAACAAAGAC TACATAGTGA CTCAGTAATA
    GAACAAGAGC CACCACAAAG CATGTCTTCT TCAGAACAAG GAGTCAACAT GGGAACAACT
    GCAGAACAAG GACTCTACAG AGAATCAATA GCAGCACAAG GACAACACAA ACGGTCAGAC
    ACAGAACAAG GACCACACAG AGGGTCAACC TCAGAACAAG GATCACGCAG ACAGTCAGTA
    ATAGAACAAG AACCACTCAA AGGGTCAACT GCAGAACAAG AGCCATCCAG AGAGATATTC
    CCAGAAAGAC AACAGGATTC AACCTTACAA TCAGGAAGAG ATAGTATTTT CAGAGAAAGT
    AAAATATTTA GGAATGCTAC TTCCTTTGAG TACACTGAAA TCTCCCCACA GAAAGAGAGG
    ACTCAGGAAC AAACCTATGA AAAAGAACTA TTTGTGAGTT CTGAGCTGCA AGAAGAAGTC
    TCAACATCAC GAAGAAAAGA TCATCTTTCA GAAACTACAA AAAAGGAAAT TCAGAAAGAT
    CCATCCTGTG AAAAGTCCCA AAAAATAGAA GGAAAGTCAT GGTCAGGTGA ACTTCTTACT
    TGTAACTGGA AGATGAATTA TTTCAAATGT GAAGATGAGG AGCAAGCCAA CTTACTCTAC
    AGTGATAACA GGTTCACAGA CCTACACTCA ATTATCAGAA ATATTCAGTC TTACAAGGAA
    GTAAAAGGTA GAAGTGCTTT CAATGGAGTT TCCTTCAATC TGCTCCAGTT TGTGCAACTT
    TTGGAGACAT TTGTTGGTGA AGATGTCCCC TTGAGTGTCA GTGAGACTCT CACAACCTTT
    TTTAAGAGGG GCTATGTTGA AACAAAAGAA GAGAAAATAA GTGGCTTAGA ACAGACTAGA
    CAAAATGCTT CCAGAGTCCG ACAGGAACTT CTCCTAGAAG CCCTGTTTCA GAAGTGGGAC
    AGTGATGGCT CAGGCTTCTT GGATCTGAAG GAAATTGATG AACTTTTGTA CACATACAAG
    GAGGGAATGG AAAAAGAATC CATGAAGAAA GCTAAACTAC ACATCCAATT TCCAAAGCCA
    CAACCTGATC AAGAAGTGAG ACTGTCTTCA AAACAATTTC AGAAATACAT AGAATTGGTT
    GTCTCTGAAC TCAGGGGCAA TGAAGATCAT GTTCTGGAAA GTATTGTGGA GTTTCTGGTG
    AATACTTTAG AAAGGAGCCA TACTGAGAAT CTGAGGAATT GTGCCAGACG AAAGTGGCTA
    TACCAAATCC AACATGCTGC AGAGACAAGT GGAGTATCCC TGGAGCCTGT GTACACTGAA
    ACCTTTAAGG CCCTCACCCA GGATGCTGAA GCCCATGGAA ATAAGAAGAT CTGTGCTCAT
    ATTTCCCTCT TAGAAGAAAA CCTACTACTG CCTGATAGAG GGGAAGTTGT ATTAAGGAAC
    GTAGCTTGTA CTTTAGATGA TGCTCCATTT GTACTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGTAAGCCAA TACACGTCCC CCAAGTTCAG
    CACCATGGGA ACGTCTTTTT CTGGAACCAT TCCCTCAAGA AGAGTGATCA AAATGGATCA
    TTCCTGGCCC TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCAT GGCTGTGGAC
    ACCCTAAGAG ATAAAAAAAA AATAAACATC TTTGTACCTC ATGAGATCAG ATTCTATCAG
    GGTGTTGCTA ATGTCTTTAG CACTGCCTAT CACTATGTCC ACAGCAGGGA GCACATCCTG
    CATGTTGTGA TTACTGGCAT TGGCTGGCTC TGTGACATCA CATCTGACAT CACCGCCATC
    ACTACATACT TCATTGAGCC TGGCCTAATG CAGGCTTCTT CCTCTCTGAG TGGTTGGACC
    AAGCTGATAA GGCCCTGGCC AAAGAGCAGA ATCACAGAGC TCTCTGGCAG AATGAACAGA
    CTGTGGAGGT GGAGAACGCA TAGTCCCGGA GACTTCCTCT TTAAATGTAC AGACAGTTCC
    GAAGTTGTCT TGGCCTCTGT CTGTGGAGAA AACCACATAC TAATTCCACT CCGTGAGAGA
    ACAGGAAAAG CTTTGGGAGT TCTTGATTTT AACATTGGCA AGAGTAAAAT GTTATTGTAT
    CGAGAATTTA AAGACCTACA GAAAATGATG AAAGTGGTCC AAGCTGCCTG CTACGAAATA
    CTGGGCGAAT TATCTGGAGA AATAAAGAAA AACTATATCC TAGAGATTGA AAATGTAGGA
    GAAGTCCAGC GGGCAGGAGT TCTCTTTTTC CGAATCATGC TACAAGAGCT GCAAGAAAGC
    CTCCGATTGC TTACCTCCAT AGACTTTGTA TCACTGTTGA TCTATGATTA TCATATTCCA
    GCAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624959.1
    CDS203..4663
    Translation

    Target ORF information:

    RefSeq Version XM_005624902.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624902.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTTTGG AAAATGGAAA AGAAGACAAA GAAAACAAAC CCGAAGAAAC TGAAGTGAAA 
    GAACCTACTG AGAGTTCACA GGGTGTACCA GGCAGTTCTG CATTAGACAC CAGCAGCAAT
    GTGGCCGAAA CAGAAGCAGA TGAAATCAAA TCCCAAGAAA TACACCTGGA AGGGCAGTCA
    AAAACTTCAG TTTGTTCAAT AAAGGACTCT AGCACTAGAG TCTCAAAGAA TTTTGTGGTT
    AGAAAACCTG CAAAAGTGAT CTTCAATTTA GATCCAACTG CGCTGAATTC AAAGCTAGAA
    CAGCCATGGA AGAAAAATTT TTTTGAAAGA TTGGAGGCAA AAGCCCAGAA AATGCAGCAG
    AAAATAATAG ATAAGGATAA CCTGAATAAA GAACTAGAAA AAAAGGCTGA AAAAAACCTC
    CCTAGGGATA ATTTGGCCAA AGAGTGGTTT AATGCTGAAA GCATAACATT GGACAGTCGT
    GCATATTTGC TGGACAAACT TCTACCTACC TTAGTTCCTG GCGTGGAAAA AATGTTAATG
    CAAGTGGAAA AGAAGAAGCT TTGGGCAGAA GGTGATATTA CAACCAAGTT TAATCCAAAT
    AATCATTTGG GGGAATACTT AATGAGAAAC AATCCTTATT ATATAAAAGA TTCAGGAATG
    TCTGGTTATC AAAGAGTGAT GAGGGATGTC ACAGAAGACC TGAAGATACA TGTTCCCAAC
    ACTGTTGGCA ACAGAGTAAC CAAGATGAAG GAGAGTATTA AACAGAAACG AGAACAAAGA
    CAATTCATAA AAGAAGTCAA AGTCAGGGTG ACCAAAACAC GGAAGCAGGC TCTGCAGGAG
    CAATTCAGTG AATGGATTCT AGACCCCAAA GGAACAATTC CTATGGTTGT GATTCAGAAT
    GTTCTTCATG AATTTTTTCA AAATCCAGAT TTACAGCTTG AAATATGTTG CAAACCAATG
    GATATTGTTG ACTCGATGGG ACCAAGATTG AACAAAACAG AATTTATAGA ATATGTCTCT
    TCATATGTTG CAGACTTTAA AAATGAAATG TTCGAGAAAC TCCTTAAGCA CTTTTGTCAT
    TGTGCAGATG AATTCCGGGA GGTTATAAAA ACAGACATGC GGAGGCAGAT ATTTGCTGAA
    CTCTTCCTGC ATTGTGACCA TGGGAAGGTA GGATTTTTGG ATCGGCAGAG GACATTGGCT
    TTGTTGGAAA CATTCTATGA CCAAAGTTCA AAAATGCTTA GAAGTTTGCT ACGAAACCCA
    AGACAATGGC CATTCATTGA ATTTGAAGAG ATAGATTTGC CTGAGTTCTG GGGAGACATG
    GATAATCAGA AACATATTTA TGAAGACTTT GACAAAGTGC TCTTAGAGAT GAATGCACAA
    CTTTCTGAAA AATATGCTAG CAAAACCCAA AGCAAATTGT TAGAAACTCC AGAAGATCAA
    CATTATCATG ATGAACATAG TGAATCAACA CTACCAGAAC AGCAGAGAGG GACAACTGCA
    GAACAAGAAC GAAACAGAAT TTTAAGCAAG GAACAAGAAC AAGGCAGAGA GTTAACTGGA
    GAAAAAGAAC TAGACAAAGA CTCAGTAACA GAACAAGAAA CCCACACAGG GTCAACTCCA
    GAACAGGGAT CAAGTAGAGA GTTAATAATA GAACAAAGAC TACATAGTGA CTCAGTAATA
    GAACAAGAGC CACCACAAAG CATGTCTTCT TCAGAACAAG GAGTCAACAT GGGAACAACT
    GCAGAACAAG GACTCTACAG AGAATCAATA GCAGCACAAG GACAACACAA ACGGTCAGAC
    ACAGAACAAG GACCACACAG AGGGTCAACC TCAGAACAAG GATCACGCAG ACAGTCAGTA
    ATAGAACAAG AACCACTCAA AGGGTCAACT GCAGAACAAG AGCCATCCAG AGAGATATTC
    CCAGAAAGAC AACAGGATTC AACCTTACAA TCAGGAAGAG ATAGTATTTT CAGAGAAAGT
    AAAATATTTA GGAATGCTAC TTCCTTTGAG TACACTGAAA TCTCCCCACA GAAAGAGAGG
    ACTCAGGAAC AAACCTATGA AAAAGAACTA TTTGTGAGTT CTGAGCTGCA AGAAGAAGTC
    TCAACATCAC GAAGAAAAGA TCATCTTTCA GAAACTACAA AAAAGGAAAT TCAGAAAGAT
    CCATCCTGTG AAAAGTCCCA AAAAATAGAA GGAAAGTCAT GGTCAGGTGA ACTTCTTACT
    TGTAACTGGA AGATGAATTA TTTCAAATGT GAAGATGAGG AGCAAGCCAA CTTACTCTAC
    AGTGATAACA GGTTCACAGA CCTACACTCA ATTATCAGAA ATATTCAGTC TTACAAGGAA
    GTAAAAGGTA GAAGTGCTTT CAATGGAGTT TCCTTCAATC TGCTCCAGTT TGTGCAACTT
    TTGGAGACAT TTGTTGGTGA AGATGTCCCC TTGAGTGTCA GTGAGACTCT CACAACCTTT
    TTTAAGAGGG GCTATGTTGA AACAAAAGAA GAGAAAATAA GTGGCTTAGA ACAGACTAGA
    CAAAATGCTT CCAGAGTCCG ACAGGAACTT CTCCTAGAAG CCCTGTTTCA GAAGTGGGAC
    AGTGATGGCT CAGGCTTCTT GGATCTGAAG GAAATTGATG AACTTTTGTA CACATACAAG
    GAGGGAATGG AAAAAGAATC CATGAAGAAA GCTAAACTAC ACATCCAATT TCCAAAGCCA
    CAACCTGATC AAGAAGTGAG ACTGTCTTCA AAACAATTTC AGAAATACAT AGAATTGGTT
    GTCTCTGAAC TCAGGGGCAA TGAAGATCAT GTTCTGGAAA GTATTGTGGA GTTTCTGGTG
    AATACTTTAG AAAGGAGCCA TACTGAGAAT CTGAGGAATT GTGCCAGACG AAAGTGGCTA
    TACCAAATCC AACATGCTGC AGAGACAAGT GGAGTATCCC TGGAGCCTGT GTACACTGAA
    ACCTTTAAGG CCCTCACCCA GGATGCTGAA GCCCATGGAA ATAAGAAGAT CTGTGCTCAT
    ATTTCCCTCT TAGAAGAAAA CCTACTACTG CCTGATAGAG GGGAAGTTGT ATTAAGGAAC
    GTAGCTTGTA CTTTAGATGA TGCTCCATTT GTACTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGTAAGCCAA TACACGTCCC CCAAGTTCAG
    CACCATGGGA ACGTCTTTTT CTGGAACCAT TCCCTCAAGA AGAGTGATCA AAATGGATCA
    TTCCTGGCCC TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCAT GGCTGTGGAC
    ACCCTAAGAG ATAAAAAAAA AATAAACATC TTTGTACCTC ATGAGATCAG ATTCTATCAG
    GGTGTTGCTA ATGTCTTTAG CACTGCCTAT CACTATGTCC ACAGCAGGGA GCACATCCTG
    CATGTTGTGA TTACTGGCAT TGGCTGGCTC TGTGACATCA CATCTGACAT CACCGCCATC
    ACTACATACT TCATTGAGCC TGGCCTAATG CAGGCTTCTT CCTCTCTGAG TGGTTGGACC
    AAGCTGATAA GGCCCTGGCC AAAGAGCAGA ATCACAGAGC TCTCTGGCAG AATGAACAGA
    CTGTGGAGGT GGAGAACGCA TAGTCCCGGA GACTTCCTCT TTAAATGTAC AGACAGTTCC
    GAAGTTGTCT TGGCCTCTGT CTGTGGAGAA AACCACATAC TAATTCCACT CCGTGAGAGA
    ACAGGAAAAG CTTTGGGAGT TCTTGATTTT AACATTGGCA AGAGTAAAAT GTTATTGTAT
    CGAGAATTTA AAGACCTACA GAAAATGATG AAAGTGGTCC AAGCTGCCTG CTACGAAATA
    CTGGGCGAAT TATCTGGAGA AATAAAGAAA AACTATATCC TAGAGATTGA AAATGTAGGA
    GAAGTCCAGC GGGCAGGAGT TCTCTTTTTC CGAATCATGC TACAAGAGCT GCAAGAAAGC
    CTCCGATTGC TTACCTCCAT AGACTTTGTA TCACTGTTGA TCTATGATTA TCATATTCCA
    GCAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12279
    Clone ID Related Accession (Same CDS sequence) XM_005624903.2 , XM_022423418.1
    Accession Version XM_005624903.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGGAGA GTATTAAACA GAAACGAGAA CAAAGACAAT TCATAAAAGA AGTCAAAGTC 
    AGGGTGACCA AAACACGGAA GCAGGCTCTG CAGGAGCAAT TCAGTGAATG GATTCTAGAC
    CCCAAAGGAA CAATTCCTAT GGTTGTGATT CAGAATGTTC TTCATGAATT TTTTCAAAAT
    CCAGATTTAC AGCTTGAAAT ATGTTGCAAA CCAATGGATA TTGTTGACTC GATGGGACCA
    AGATTGAACA AAACAGAATT TATAGAATAT GTCTCTTCAT ATGTTGCAGA CTTTAAAAAT
    GAAATGTTCG AGAAACTCCT TAAGCACTTT TGTCATTGTG CAGATGAATT CCGGGAGGTT
    ATAAAAACAG ACATGCGGAG GCAGATATTT GCTGAACTCT TCCTGCATTG TGACCATGGG
    AAGGTAGGAT TTTTGGATCG GCAGAGGACA TTGGCTTTGT TGGAAACATT CTATGACCAA
    AGTTCAAAAA TGCTTAGAAG TTTGCTACGA AACCCAAGAC AATGGCCATT CATTGAATTT
    GAAGAGATAG ATTTGCCTGA GTTCTGGGGA GACATGGATA ATCAGAAACA TATTTATGAA
    GACTTTGACA AAGTGCTCTT AGAGATGAAT GCACAACTTT CTGAAAAATA TGCTAGCAAA
    ACCCAAAGCA AATTGTTAGA AACTCCAGAA GATCAACATT ATCATGATGA ACATAGTGAA
    TCAACACTAC CAGAACAGCA GAGAGGGACA ACTGCAGAAC AAGAACGAAA CAGAATTTTA
    AGCAAGGAAC AAGAACAAGG CAGAGAGTTA ACTGGAGAAA AAGAACTAGA CAAAGACTCA
    GTAACAGAAC AAGAAACCCA CACAGGGTCA ACTCCAGAAC AGGGATCAAG TAGAGAGTTA
    ATAATAGAAC AAAGACTACA TAGTGACTCA GTAATAGAAC AAGAGCCACC ACAAAGCATG
    TCTTCTTCAG AACAAGGAGT CAACATGGGA ACAACTGCAG AACAAGGACT CTACAGAGAA
    TCAATAGCAG CACAAGGACA ACACAAACGG TCAGACACAG AACAAGGACC ACACAGAGGG
    TCAACCTCAG AACAAGGATC ACGCAGACAG TCAGTAATAG AACAAGAACC ACTCAAAGGG
    TCAACTGCAG AACAAGAGCC ATCCAGAGAG ATATTCCCAG AAAGACAACA GGATTCAACC
    TTACAATCAG GAAGAGATAG TATTTTCAGA GAAAGTAAAA TATTTAGGAA TGCTACTTCC
    TTTGAGTACA CTGAAATCTC CCCACAGAAA GAGAGGACTC AGGAACAAAC CTATGAAAAA
    GAACTATTTG TGAGTTCTGA GCTGCAAGAA GAAGTCTCAA CATCACGAAG AAAAGATCAT
    CTTTCAGAAA CTACAAAAAA GGAAATTCAG AAAGATCCAT CCTGTGAAAA GTCCCAAAAA
    ATAGAAGGAA AGTCATGGTC AGGTGAACTT CTTACTTGTA ACTGGAAGAT GAATTATTTC
    AAATGTGAAG ATGAGGAGCA AGCCAACTTA CTCTACAGTG ATAACAGGTT CACAGACCTA
    CACTCAATTA TCAGAAATAT TCAGTCTTAC AAGGAAGTAA AAGGTAGAAG TGCTTTCAAT
    GGAGTTTCCT TCAATCTGCT CCAGTTTGTG CAACTTTTGG AGACATTTGT TGGTGAAGAT
    GTCCCCTTGA GTGTCAGTGA GACTCTCACA ACCTTTTTTA AGAGGGGCTA TGTTGAAACA
    AAAGAAGAGA AAATAAGTGG CTTAGAACAG ACTAGACAAA ATGCTTCCAG AGTCCGACAG
    GAACTTCTCC TAGAAGCCCT GTTTCAGAAG TGGGACAGTG ATGGCTCAGG CTTCTTGGAT
    CTGAAGGAAA TTGATGAACT TTTGTACACA TACAAGGAGG GAATGGAAAA AGAATCCATG
    AAGAAAGCTA AACTACACAT CCAATTTCCA AAGCCACAAC CTGATCAAGA AGTGAGACTG
    TCTTCAAAAC AATTTCAGAA ATACATAGAA TTGGTTGTCT CTGAACTCAG GGGCAATGAA
    GATCATGTTC TGGAAAGTAT TGTGGAGTTT CTGGTGAATA CTTTAGAAAG GAGCCATACT
    GAGAATCTGA GGAATTGTGC CAGACGAAAG TGGCTATACC AAATCCAACA TGCTGCAGAG
    ACAAGTGGAG TATCCCTGGA GCCTGTGTAC ACTGAAACCT TTAAGGCCCT CACCCAGGAT
    GCTGAAGCCC ATGGAAATAA GAAGATCTGT GCTCATATTT CCCTCTTAGA AGAAAACCTA
    CTACTGCCTG ATAGAGGGGA AGTTGTATTA AGGAACGTAG CTTGTACTTT AGATGATGCT
    CCATTTGTAC TGAACAAAGT GCTCTACAGG GACATGAAGG GAATCAGCTT TACAGTAGTG
    GATGAAGGTA AGCCAATACA CGTCCCCCAA GTTCAGCACC ATGGGAACGT CTTTTTCTGG
    AACCATTCCC TCAAGAAGAG TGATCAAAAT GGATCATTCC TGGCCCTGCC TCTTCAGGAT
    GCATACATGA GGATCTTTGG GGTCATGGCT GTGGACACCC TAAGAGATAA AAAAAAAATA
    AACATCTTTG TACCTCATGA GATCAGATTC TATCAGGGTG TTGCTAATGT CTTTAGCACT
    GCCTATCACT ATGTCCACAG CAGGGAGCAC ATCCTGCATG TTGTGATTAC TGGCATTGGC
    TGGCTCTGTG ACATCACATC TGACATCACC GCCATCACTA CATACTTCAT TGAGCCTGGC
    CTAATGCAGG CTTCTTCCTC TCTGAGTGGT TGGACCAAGC TGATAAGGCC CTGGCCAAAG
    AGCAGAATCA CAGAGCTCTC TGGCAGAATG AACAGACTGT GGAGGTGGAG AACGCATAGT
    CCCGGAGACT TCCTCTTTAA ATGTACAGAC AGTTCCGAAG TTGTCTTGGC CTCTGTCTGT
    GGAGAAAACC ACATACTAAT TCCACTCCGT GAGAGAACAG GAAAAGCTTT GGGAGTTCTT
    GATTTTAACA TTGGCAAGAG TAAAATGTTA TTGTATCGAG AATTTAAAGA CCTACAGAAA
    ATGATGAAAG TGGTCCAAGC TGCCTGCTAC GAAATACTGG GCGAATTATC TGGAGAAATA
    AAGAAAAACT ATATCCTAGA GATTGAAAAT GTAGGAGAAG TCCAGCGGGC AGGAGTTCTC
    TTTTTCCGAA TCATGCTACA AGAGCTGCAA GAAAGCCTCC GATTGCTTAC CTCCATAGAC
    TTTGTATCAC TGTTGATCTA TGATTATCAT ATTCCAGCAG AGTCCGTATC TCTACCAGAC
    AGCAAGACCC AGGAGTTGGA AGCCAACATA AAGCTAGTAC AGGACATCCT GATAGGGGTT
    ATTTTGTTCA TCCATCCAGA GTTGGAATTA TCAAGTGAGT TTGGAAATTG GGAGAAGTGT
    AAACTGTATG TTAGCAGATA TTTAGTCGAG AATATTTGTG ACTTTGATCC AACTGCTAAG
    CATGTGGAAG TTAATTTACA GCTCATTGGT GAATATATCA GAGGTCATTC TCGAATTGAA
    GTATGGAAAT TTGGTAATAT TGTCATCGAA TACCTATACC ACTGGGCACA CATCTGCTTA
    GCTCTCATGG AGCTAAACAA AAAACCAAGC AGTGCTATTT TACCCCCATT GCCCTCCAAA
    ACTGACTCCT GTGTTTATGC AAAAATGCCA AAGGGAAACT TGCCAGGGAA ATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624960.1
    CDS94..3810
    Translation

    Target ORF information:

    RefSeq Version XM_005624903.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624903.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGGAGA GTATTAAACA GAAACGAGAA CAAAGACAAT TCATAAAAGA AGTCAAAGTC 
    AGGGTGACCA AAACACGGAA GCAGGCTCTG CAGGAGCAAT TCAGTGAATG GATTCTAGAC
    CCCAAAGGAA CAATTCCTAT GGTTGTGATT CAGAATGTTC TTCATGAATT TTTTCAAAAT
    CCAGATTTAC AGCTTGAAAT ATGTTGCAAA CCAATGGATA TTGTTGACTC GATGGGACCA
    AGATTGAACA AAACAGAATT TATAGAATAT GTCTCTTCAT ATGTTGCAGA CTTTAAAAAT
    GAAATGTTCG AGAAACTCCT TAAGCACTTT TGTCATTGTG CAGATGAATT CCGGGAGGTT
    ATAAAAACAG ACATGCGGAG GCAGATATTT GCTGAACTCT TCCTGCATTG TGACCATGGG
    AAGGTAGGAT TTTTGGATCG GCAGAGGACA TTGGCTTTGT TGGAAACATT CTATGACCAA
    AGTTCAAAAA TGCTTAGAAG TTTGCTACGA AACCCAAGAC AATGGCCATT CATTGAATTT
    GAAGAGATAG ATTTGCCTGA GTTCTGGGGA GACATGGATA ATCAGAAACA TATTTATGAA
    GACTTTGACA AAGTGCTCTT AGAGATGAAT GCACAACTTT CTGAAAAATA TGCTAGCAAA
    ACCCAAAGCA AATTGTTAGA AACTCCAGAA GATCAACATT ATCATGATGA ACATAGTGAA
    TCAACACTAC CAGAACAGCA GAGAGGGACA ACTGCAGAAC AAGAACGAAA CAGAATTTTA
    AGCAAGGAAC AAGAACAAGG CAGAGAGTTA ACTGGAGAAA AAGAACTAGA CAAAGACTCA
    GTAACAGAAC AAGAAACCCA CACAGGGTCA ACTCCAGAAC AGGGATCAAG TAGAGAGTTA
    ATAATAGAAC AAAGACTACA TAGTGACTCA GTAATAGAAC AAGAGCCACC ACAAAGCATG
    TCTTCTTCAG AACAAGGAGT CAACATGGGA ACAACTGCAG AACAAGGACT CTACAGAGAA
    TCAATAGCAG CACAAGGACA ACACAAACGG TCAGACACAG AACAAGGACC ACACAGAGGG
    TCAACCTCAG AACAAGGATC ACGCAGACAG TCAGTAATAG AACAAGAACC ACTCAAAGGG
    TCAACTGCAG AACAAGAGCC ATCCAGAGAG ATATTCCCAG AAAGACAACA GGATTCAACC
    TTACAATCAG GAAGAGATAG TATTTTCAGA GAAAGTAAAA TATTTAGGAA TGCTACTTCC
    TTTGAGTACA CTGAAATCTC CCCACAGAAA GAGAGGACTC AGGAACAAAC CTATGAAAAA
    GAACTATTTG TGAGTTCTGA GCTGCAAGAA GAAGTCTCAA CATCACGAAG AAAAGATCAT
    CTTTCAGAAA CTACAAAAAA GGAAATTCAG AAAGATCCAT CCTGTGAAAA GTCCCAAAAA
    ATAGAAGGAA AGTCATGGTC AGGTGAACTT CTTACTTGTA ACTGGAAGAT GAATTATTTC
    AAATGTGAAG ATGAGGAGCA AGCCAACTTA CTCTACAGTG ATAACAGGTT CACAGACCTA
    CACTCAATTA TCAGAAATAT TCAGTCTTAC AAGGAAGTAA AAGGTAGAAG TGCTTTCAAT
    GGAGTTTCCT TCAATCTGCT CCAGTTTGTG CAACTTTTGG AGACATTTGT TGGTGAAGAT
    GTCCCCTTGA GTGTCAGTGA GACTCTCACA ACCTTTTTTA AGAGGGGCTA TGTTGAAACA
    AAAGAAGAGA AAATAAGTGG CTTAGAACAG ACTAGACAAA ATGCTTCCAG AGTCCGACAG
    GAACTTCTCC TAGAAGCCCT GTTTCAGAAG TGGGACAGTG ATGGCTCAGG CTTCTTGGAT
    CTGAAGGAAA TTGATGAACT TTTGTACACA TACAAGGAGG GAATGGAAAA AGAATCCATG
    AAGAAAGCTA AACTACACAT CCAATTTCCA AAGCCACAAC CTGATCAAGA AGTGAGACTG
    TCTTCAAAAC AATTTCAGAA ATACATAGAA TTGGTTGTCT CTGAACTCAG GGGCAATGAA
    GATCATGTTC TGGAAAGTAT TGTGGAGTTT CTGGTGAATA CTTTAGAAAG GAGCCATACT
    GAGAATCTGA GGAATTGTGC CAGACGAAAG TGGCTATACC AAATCCAACA TGCTGCAGAG
    ACAAGTGGAG TATCCCTGGA GCCTGTGTAC ACTGAAACCT TTAAGGCCCT CACCCAGGAT
    GCTGAAGCCC ATGGAAATAA GAAGATCTGT GCTCATATTT CCCTCTTAGA AGAAAACCTA
    CTACTGCCTG ATAGAGGGGA AGTTGTATTA AGGAACGTAG CTTGTACTTT AGATGATGCT
    CCATTTGTAC TGAACAAAGT GCTCTACAGG GACATGAAGG GAATCAGCTT TACAGTAGTG
    GATGAAGGTA AGCCAATACA CGTCCCCCAA GTTCAGCACC ATGGGAACGT CTTTTTCTGG
    AACCATTCCC TCAAGAAGAG TGATCAAAAT GGATCATTCC TGGCCCTGCC TCTTCAGGAT
    GCATACATGA GGATCTTTGG GGTCATGGCT GTGGACACCC TAAGAGATAA AAAAAAAATA
    AACATCTTTG TACCTCATGA GATCAGATTC TATCAGGGTG TTGCTAATGT CTTTAGCACT
    GCCTATCACT ATGTCCACAG CAGGGAGCAC ATCCTGCATG TTGTGATTAC TGGCATTGGC
    TGGCTCTGTG ACATCACATC TGACATCACC GCCATCACTA CATACTTCAT TGAGCCTGGC
    CTAATGCAGG CTTCTTCCTC TCTGAGTGGT TGGACCAAGC TGATAAGGCC CTGGCCAAAG
    AGCAGAATCA CAGAGCTCTC TGGCAGAATG AACAGACTGT GGAGGTGGAG AACGCATAGT
    CCCGGAGACT TCCTCTTTAA ATGTACAGAC AGTTCCGAAG TTGTCTTGGC CTCTGTCTGT
    GGAGAAAACC ACATACTAAT TCCACTCCGT GAGAGAACAG GAAAAGCTTT GGGAGTTCTT
    GATTTTAACA TTGGCAAGAG TAAAATGTTA TTGTATCGAG AATTTAAAGA CCTACAGAAA
    ATGATGAAAG TGGTCCAAGC TGCCTGCTAC GAAATACTGG GCGAATTATC TGGAGAAATA
    AAGAAAAACT ATATCCTAGA GATTGAAAAT GTAGGAGAAG TCCAGCGGGC AGGAGTTCTC
    TTTTTCCGAA TCATGCTACA AGAGCTGCAA GAAAGCCTCC GATTGCTTAC CTCCATAGAC
    TTTGTATCAC TGTTGATCTA TGATTATCAT ATTCCAGCAG AGTCCGTATC TCTACCAGAC
    AGCAAGACCC AGGAGTTGGA AGCCAACATA AAGCTAGTAC AGGACATCCT GATAGGGGTT
    ATTTTGTTCA TCCATCCAGA GTTGGAATTA TCAAGTGAGT TTGGAAATTG GGAGAAGTGT
    AAACTGTATG TTAGCAGATA TTTAGTCGAG AATATTTGTG ACTTTGATCC AACTGCTAAG
    CATGTGGAAG TTAATTTACA GCTCATTGGT GAATATATCA GAGGTCATTC TCGAATTGAA
    GTATGGAAAT TTGGTAATAT TGTCATCGAA TACCTATACC ACTGGGCACA CATCTGCTTA
    GCTCTCATGG AGCTAAACAA AAAACCAAGC AGTGCTATTT TACCCCCATT GCCCTCCAAA
    ACTGACTCCT GTGTTTATGC AAAAATGCCA AAGGGAAACT TGCCAGGGAA ATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12279
    Clone ID Related Accession (Same CDS sequence) XM_005624903.2 , XM_022423418.1
    Accession Version XM_022423418.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGGAGA GTATTAAACA GAAACGAGAA CAAAGACAAT TCATAAAAGA AGTCAAAGTC 
    AGGGTGACCA AAACACGGAA GCAGGCTCTG CAGGAGCAAT TCAGTGAATG GATTCTAGAC
    CCCAAAGGAA CAATTCCTAT GGTTGTGATT CAGAATGTTC TTCATGAATT TTTTCAAAAT
    CCAGATTTAC AGCTTGAAAT ATGTTGCAAA CCAATGGATA TTGTTGACTC GATGGGACCA
    AGATTGAACA AAACAGAATT TATAGAATAT GTCTCTTCAT ATGTTGCAGA CTTTAAAAAT
    GAAATGTTCG AGAAACTCCT TAAGCACTTT TGTCATTGTG CAGATGAATT CCGGGAGGTT
    ATAAAAACAG ACATGCGGAG GCAGATATTT GCTGAACTCT TCCTGCATTG TGACCATGGG
    AAGGTAGGAT TTTTGGATCG GCAGAGGACA TTGGCTTTGT TGGAAACATT CTATGACCAA
    AGTTCAAAAA TGCTTAGAAG TTTGCTACGA AACCCAAGAC AATGGCCATT CATTGAATTT
    GAAGAGATAG ATTTGCCTGA GTTCTGGGGA GACATGGATA ATCAGAAACA TATTTATGAA
    GACTTTGACA AAGTGCTCTT AGAGATGAAT GCACAACTTT CTGAAAAATA TGCTAGCAAA
    ACCCAAAGCA AATTGTTAGA AACTCCAGAA GATCAACATT ATCATGATGA ACATAGTGAA
    TCAACACTAC CAGAACAGCA GAGAGGGACA ACTGCAGAAC AAGAACGAAA CAGAATTTTA
    AGCAAGGAAC AAGAACAAGG CAGAGAGTTA ACTGGAGAAA AAGAACTAGA CAAAGACTCA
    GTAACAGAAC AAGAAACCCA CACAGGGTCA ACTCCAGAAC AGGGATCAAG TAGAGAGTTA
    ATAATAGAAC AAAGACTACA TAGTGACTCA GTAATAGAAC AAGAGCCACC ACAAAGCATG
    TCTTCTTCAG AACAAGGAGT CAACATGGGA ACAACTGCAG AACAAGGACT CTACAGAGAA
    TCAATAGCAG CACAAGGACA ACACAAACGG TCAGACACAG AACAAGGACC ACACAGAGGG
    TCAACCTCAG AACAAGGATC ACGCAGACAG TCAGTAATAG AACAAGAACC ACTCAAAGGG
    TCAACTGCAG AACAAGAGCC ATCCAGAGAG ATATTCCCAG AAAGACAACA GGATTCAACC
    TTACAATCAG GAAGAGATAG TATTTTCAGA GAAAGTAAAA TATTTAGGAA TGCTACTTCC
    TTTGAGTACA CTGAAATCTC CCCACAGAAA GAGAGGACTC AGGAACAAAC CTATGAAAAA
    GAACTATTTG TGAGTTCTGA GCTGCAAGAA GAAGTCTCAA CATCACGAAG AAAAGATCAT
    CTTTCAGAAA CTACAAAAAA GGAAATTCAG AAAGATCCAT CCTGTGAAAA GTCCCAAAAA
    ATAGAAGGAA AGTCATGGTC AGGTGAACTT CTTACTTGTA ACTGGAAGAT GAATTATTTC
    AAATGTGAAG ATGAGGAGCA AGCCAACTTA CTCTACAGTG ATAACAGGTT CACAGACCTA
    CACTCAATTA TCAGAAATAT TCAGTCTTAC AAGGAAGTAA AAGGTAGAAG TGCTTTCAAT
    GGAGTTTCCT TCAATCTGCT CCAGTTTGTG CAACTTTTGG AGACATTTGT TGGTGAAGAT
    GTCCCCTTGA GTGTCAGTGA GACTCTCACA ACCTTTTTTA AGAGGGGCTA TGTTGAAACA
    AAAGAAGAGA AAATAAGTGG CTTAGAACAG ACTAGACAAA ATGCTTCCAG AGTCCGACAG
    GAACTTCTCC TAGAAGCCCT GTTTCAGAAG TGGGACAGTG ATGGCTCAGG CTTCTTGGAT
    CTGAAGGAAA TTGATGAACT TTTGTACACA TACAAGGAGG GAATGGAAAA AGAATCCATG
    AAGAAAGCTA AACTACACAT CCAATTTCCA AAGCCACAAC CTGATCAAGA AGTGAGACTG
    TCTTCAAAAC AATTTCAGAA ATACATAGAA TTGGTTGTCT CTGAACTCAG GGGCAATGAA
    GATCATGTTC TGGAAAGTAT TGTGGAGTTT CTGGTGAATA CTTTAGAAAG GAGCCATACT
    GAGAATCTGA GGAATTGTGC CAGACGAAAG TGGCTATACC AAATCCAACA TGCTGCAGAG
    ACAAGTGGAG TATCCCTGGA GCCTGTGTAC ACTGAAACCT TTAAGGCCCT CACCCAGGAT
    GCTGAAGCCC ATGGAAATAA GAAGATCTGT GCTCATATTT CCCTCTTAGA AGAAAACCTA
    CTACTGCCTG ATAGAGGGGA AGTTGTATTA AGGAACGTAG CTTGTACTTT AGATGATGCT
    CCATTTGTAC TGAACAAAGT GCTCTACAGG GACATGAAGG GAATCAGCTT TACAGTAGTG
    GATGAAGGTA AGCCAATACA CGTCCCCCAA GTTCAGCACC ATGGGAACGT CTTTTTCTGG
    AACCATTCCC TCAAGAAGAG TGATCAAAAT GGATCATTCC TGGCCCTGCC TCTTCAGGAT
    GCATACATGA GGATCTTTGG GGTCATGGCT GTGGACACCC TAAGAGATAA AAAAAAAATA
    AACATCTTTG TACCTCATGA GATCAGATTC TATCAGGGTG TTGCTAATGT CTTTAGCACT
    GCCTATCACT ATGTCCACAG CAGGGAGCAC ATCCTGCATG TTGTGATTAC TGGCATTGGC
    TGGCTCTGTG ACATCACATC TGACATCACC GCCATCACTA CATACTTCAT TGAGCCTGGC
    CTAATGCAGG CTTCTTCCTC TCTGAGTGGT TGGACCAAGC TGATAAGGCC CTGGCCAAAG
    AGCAGAATCA CAGAGCTCTC TGGCAGAATG AACAGACTGT GGAGGTGGAG AACGCATAGT
    CCCGGAGACT TCCTCTTTAA ATGTACAGAC AGTTCCGAAG TTGTCTTGGC CTCTGTCTGT
    GGAGAAAACC ACATACTAAT TCCACTCCGT GAGAGAACAG GAAAAGCTTT GGGAGTTCTT
    GATTTTAACA TTGGCAAGAG TAAAATGTTA TTGTATCGAG AATTTAAAGA CCTACAGAAA
    ATGATGAAAG TGGTCCAAGC TGCCTGCTAC GAAATACTGG GCGAATTATC TGGAGAAATA
    AAGAAAAACT ATATCCTAGA GATTGAAAAT GTAGGAGAAG TCCAGCGGGC AGGAGTTCTC
    TTTTTCCGAA TCATGCTACA AGAGCTGCAA GAAAGCCTCC GATTGCTTAC CTCCATAGAC
    TTTGTATCAC TGTTGATCTA TGATTATCAT ATTCCAGCAG AGTCCGTATC TCTACCAGAC
    AGCAAGACCC AGGAGTTGGA AGCCAACATA AAGCTAGTAC AGGACATCCT GATAGGGGTT
    ATTTTGTTCA TCCATCCAGA GTTGGAATTA TCAAGTGAGT TTGGAAATTG GGAGAAGTGT
    AAACTGTATG TTAGCAGATA TTTAGTCGAG AATATTTGTG ACTTTGATCC AACTGCTAAG
    CATGTGGAAG TTAATTTACA GCTCATTGGT GAATATATCA GAGGTCATTC TCGAATTGAA
    GTATGGAAAT TTGGTAATAT TGTCATCGAA TACCTATACC ACTGGGCACA CATCTGCTTA
    GCTCTCATGG AGCTAAACAA AAAACCAAGC AGTGCTATTT TACCCCCATT GCCCTCCAAA
    ACTGACTCCT GTGTTTATGC AAAAATGCCA AAGGGAAACT TGCCAGGGAA ATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279126.1
    CDS315..4031
    Translation

    Target ORF information:

    RefSeq Version XM_022423418.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423418.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGGAGA GTATTAAACA GAAACGAGAA CAAAGACAAT TCATAAAAGA AGTCAAAGTC 
    AGGGTGACCA AAACACGGAA GCAGGCTCTG CAGGAGCAAT TCAGTGAATG GATTCTAGAC
    CCCAAAGGAA CAATTCCTAT GGTTGTGATT CAGAATGTTC TTCATGAATT TTTTCAAAAT
    CCAGATTTAC AGCTTGAAAT ATGTTGCAAA CCAATGGATA TTGTTGACTC GATGGGACCA
    AGATTGAACA AAACAGAATT TATAGAATAT GTCTCTTCAT ATGTTGCAGA CTTTAAAAAT
    GAAATGTTCG AGAAACTCCT TAAGCACTTT TGTCATTGTG CAGATGAATT CCGGGAGGTT
    ATAAAAACAG ACATGCGGAG GCAGATATTT GCTGAACTCT TCCTGCATTG TGACCATGGG
    AAGGTAGGAT TTTTGGATCG GCAGAGGACA TTGGCTTTGT TGGAAACATT CTATGACCAA
    AGTTCAAAAA TGCTTAGAAG TTTGCTACGA AACCCAAGAC AATGGCCATT CATTGAATTT
    GAAGAGATAG ATTTGCCTGA GTTCTGGGGA GACATGGATA ATCAGAAACA TATTTATGAA
    GACTTTGACA AAGTGCTCTT AGAGATGAAT GCACAACTTT CTGAAAAATA TGCTAGCAAA
    ACCCAAAGCA AATTGTTAGA AACTCCAGAA GATCAACATT ATCATGATGA ACATAGTGAA
    TCAACACTAC CAGAACAGCA GAGAGGGACA ACTGCAGAAC AAGAACGAAA CAGAATTTTA
    AGCAAGGAAC AAGAACAAGG CAGAGAGTTA ACTGGAGAAA AAGAACTAGA CAAAGACTCA
    GTAACAGAAC AAGAAACCCA CACAGGGTCA ACTCCAGAAC AGGGATCAAG TAGAGAGTTA
    ATAATAGAAC AAAGACTACA TAGTGACTCA GTAATAGAAC AAGAGCCACC ACAAAGCATG
    TCTTCTTCAG AACAAGGAGT CAACATGGGA ACAACTGCAG AACAAGGACT CTACAGAGAA
    TCAATAGCAG CACAAGGACA ACACAAACGG TCAGACACAG AACAAGGACC ACACAGAGGG
    TCAACCTCAG AACAAGGATC ACGCAGACAG TCAGTAATAG AACAAGAACC ACTCAAAGGG
    TCAACTGCAG AACAAGAGCC ATCCAGAGAG ATATTCCCAG AAAGACAACA GGATTCAACC
    TTACAATCAG GAAGAGATAG TATTTTCAGA GAAAGTAAAA TATTTAGGAA TGCTACTTCC
    TTTGAGTACA CTGAAATCTC CCCACAGAAA GAGAGGACTC AGGAACAAAC CTATGAAAAA
    GAACTATTTG TGAGTTCTGA GCTGCAAGAA GAAGTCTCAA CATCACGAAG AAAAGATCAT
    CTTTCAGAAA CTACAAAAAA GGAAATTCAG AAAGATCCAT CCTGTGAAAA GTCCCAAAAA
    ATAGAAGGAA AGTCATGGTC AGGTGAACTT CTTACTTGTA ACTGGAAGAT GAATTATTTC
    AAATGTGAAG ATGAGGAGCA AGCCAACTTA CTCTACAGTG ATAACAGGTT CACAGACCTA
    CACTCAATTA TCAGAAATAT TCAGTCTTAC AAGGAAGTAA AAGGTAGAAG TGCTTTCAAT
    GGAGTTTCCT TCAATCTGCT CCAGTTTGTG CAACTTTTGG AGACATTTGT TGGTGAAGAT
    GTCCCCTTGA GTGTCAGTGA GACTCTCACA ACCTTTTTTA AGAGGGGCTA TGTTGAAACA
    AAAGAAGAGA AAATAAGTGG CTTAGAACAG ACTAGACAAA ATGCTTCCAG AGTCCGACAG
    GAACTTCTCC TAGAAGCCCT GTTTCAGAAG TGGGACAGTG ATGGCTCAGG CTTCTTGGAT
    CTGAAGGAAA TTGATGAACT TTTGTACACA TACAAGGAGG GAATGGAAAA AGAATCCATG
    AAGAAAGCTA AACTACACAT CCAATTTCCA AAGCCACAAC CTGATCAAGA AGTGAGACTG
    TCTTCAAAAC AATTTCAGAA ATACATAGAA TTGGTTGTCT CTGAACTCAG GGGCAATGAA
    GATCATGTTC TGGAAAGTAT TGTGGAGTTT CTGGTGAATA CTTTAGAAAG GAGCCATACT
    GAGAATCTGA GGAATTGTGC CAGACGAAAG TGGCTATACC AAATCCAACA TGCTGCAGAG
    ACAAGTGGAG TATCCCTGGA GCCTGTGTAC ACTGAAACCT TTAAGGCCCT CACCCAGGAT
    GCTGAAGCCC ATGGAAATAA GAAGATCTGT GCTCATATTT CCCTCTTAGA AGAAAACCTA
    CTACTGCCTG ATAGAGGGGA AGTTGTATTA AGGAACGTAG CTTGTACTTT AGATGATGCT
    CCATTTGTAC TGAACAAAGT GCTCTACAGG GACATGAAGG GAATCAGCTT TACAGTAGTG
    GATGAAGGTA AGCCAATACA CGTCCCCCAA GTTCAGCACC ATGGGAACGT CTTTTTCTGG
    AACCATTCCC TCAAGAAGAG TGATCAAAAT GGATCATTCC TGGCCCTGCC TCTTCAGGAT
    GCATACATGA GGATCTTTGG GGTCATGGCT GTGGACACCC TAAGAGATAA AAAAAAAATA
    AACATCTTTG TACCTCATGA GATCAGATTC TATCAGGGTG TTGCTAATGT CTTTAGCACT
    GCCTATCACT ATGTCCACAG CAGGGAGCAC ATCCTGCATG TTGTGATTAC TGGCATTGGC
    TGGCTCTGTG ACATCACATC TGACATCACC GCCATCACTA CATACTTCAT TGAGCCTGGC
    CTAATGCAGG CTTCTTCCTC TCTGAGTGGT TGGACCAAGC TGATAAGGCC CTGGCCAAAG
    AGCAGAATCA CAGAGCTCTC TGGCAGAATG AACAGACTGT GGAGGTGGAG AACGCATAGT
    CCCGGAGACT TCCTCTTTAA ATGTACAGAC AGTTCCGAAG TTGTCTTGGC CTCTGTCTGT
    GGAGAAAACC ACATACTAAT TCCACTCCGT GAGAGAACAG GAAAAGCTTT GGGAGTTCTT
    GATTTTAACA TTGGCAAGAG TAAAATGTTA TTGTATCGAG AATTTAAAGA CCTACAGAAA
    ATGATGAAAG TGGTCCAAGC TGCCTGCTAC GAAATACTGG GCGAATTATC TGGAGAAATA
    AAGAAAAACT ATATCCTAGA GATTGAAAAT GTAGGAGAAG TCCAGCGGGC AGGAGTTCTC
    TTTTTCCGAA TCATGCTACA AGAGCTGCAA GAAAGCCTCC GATTGCTTAC CTCCATAGAC
    TTTGTATCAC TGTTGATCTA TGATTATCAT ATTCCAGCAG AGTCCGTATC TCTACCAGAC
    AGCAAGACCC AGGAGTTGGA AGCCAACATA AAGCTAGTAC AGGACATCCT GATAGGGGTT
    ATTTTGTTCA TCCATCCAGA GTTGGAATTA TCAAGTGAGT TTGGAAATTG GGAGAAGTGT
    AAACTGTATG TTAGCAGATA TTTAGTCGAG AATATTTGTG ACTTTGATCC AACTGCTAAG
    CATGTGGAAG TTAATTTACA GCTCATTGGT GAATATATCA GAGGTCATTC TCGAATTGAA
    GTATGGAAAT TTGGTAATAT TGTCATCGAA TACCTATACC ACTGGGCACA CATCTGCTTA
    GCTCTCATGG AGCTAAACAA AAAACCAAGC AGTGCTATTT TACCCCCATT GCCCTCCAAA
    ACTGACTCCT GTGTTTATGC AAAAATGCCA AAGGGAAACT TGCCAGGGAA ATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290829
    Clone ID Related Accession (Same CDS sequence) XM_022423408.1
    Accession Version XM_022423408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGATT CAGACTATGT TTTACGCAAT ATGATGGTTA CCAGACACCT GGGTCTAACA
    GAAATCCACA GGAACCCCCC TATAATTTTC AGGAAGACAT GCATCTTCAG AGACTTCCTC
    TTTAAATGTA CAGACAGTTC CGAAGTTGTC TTGGCCTCTG TCTGTGGAGA AAACCACATA
    CTAATTCCAC TCCGTGAGAG AACAGGAAAA GCTTTGGGAG TTCTTGATTT TAACATTGGC
    AAGAGTAAAA TGTTATTGTA TCGAGAATTT AAAGACCTAC AGAAAATGAT GAAAGTGGTC
    CAAGCTGCCT GCTACGAAAT ACTGGGCGAA TTATCTGGAG AAATAAAGAA AAACTATATC
    CTAGAGATTG AAAATGTAGG AGAAGTCCAG CGGGCAGGAG TTCTCTTTTT CCGAATCATG
    CTACAAGAGC TGCAAGAAAG CCTCCGATTG CTTACCTCCA TAGACTTTGT ATCACTGTTG
    ATCTATGATT ATCATATTCC AGCAGAGTCC GTATCTCTAC CAGACAGCAA GACCCAGGAG
    TTGGAAGCCA ACATAAAGCT AGTACAGGAC ATCCTGATAG GGGTTATTTT GTTCATCCAT
    CCAGAGTTGG AATTATCAAG TGAGTTTGGA AATTGGGAGA AGTGTAAACT GTATGTTAGC
    AGATATTTAG TCGAGAATAT TTGTGACTTT GATCCAACTG CTAAGCATGT GGAAGTTAAT
    TTACAGCTCA TTGGTGAATA TATCAGAGGT CATTCTCGAA TTGAAGTATG GAAATTTGGT
    AATATTGTCA TCGAATACCT ATACCACTGG GCACACATCT GCTTAGCTCT CATGGAGCTA
    AACAAAAAAC CAAGCAGTGC TATTTTACCC CCATTGCCCT CCAAAACTGA CTCCTGTGTT
    TATGCAAAAA TGCCAAAGGG AAACTTGCCA GGGAAATGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279116.1
    CDS220..4701
    Translation

    Target ORF information:

    RefSeq Version XM_022423408.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGATT CAGACTATGT TTTACGCAAT ATGATGGTTA CCAGACACCT GGGTCTAACA
    GAAATCCACA GGAACCCCCC TATAATTTTC AGGAAGACAT GCATCTTCAG AGACTTCCTC
    TTTAAATGTA CAGACAGTTC CGAAGTTGTC TTGGCCTCTG TCTGTGGAGA AAACCACATA
    CTAATTCCAC TCCGTGAGAG AACAGGAAAA GCTTTGGGAG TTCTTGATTT TAACATTGGC
    AAGAGTAAAA TGTTATTGTA TCGAGAATTT AAAGACCTAC AGAAAATGAT GAAAGTGGTC
    CAAGCTGCCT GCTACGAAAT ACTGGGCGAA TTATCTGGAG AAATAAAGAA AAACTATATC
    CTAGAGATTG AAAATGTAGG AGAAGTCCAG CGGGCAGGAG TTCTCTTTTT CCGAATCATG
    CTACAAGAGC TGCAAGAAAG CCTCCGATTG CTTACCTCCA TAGACTTTGT ATCACTGTTG
    ATCTATGATT ATCATATTCC AGCAGAGTCC GTATCTCTAC CAGACAGCAA GACCCAGGAG
    TTGGAAGCCA ACATAAAGCT AGTACAGGAC ATCCTGATAG GGGTTATTTT GTTCATCCAT
    CCAGAGTTGG AATTATCAAG TGAGTTTGGA AATTGGGAGA AGTGTAAACT GTATGTTAGC
    AGATATTTAG TCGAGAATAT TTGTGACTTT GATCCAACTG CTAAGCATGT GGAAGTTAAT
    TTACAGCTCA TTGGTGAATA TATCAGAGGT CATTCTCGAA TTGAAGTATG GAAATTTGGT
    AATATTGTCA TCGAATACCT ATACCACTGG GCACACATCT GCTTAGCTCT CATGGAGCTA
    AACAAAAAAC CAAGCAGTGC TATTTTACCC CCATTGCCCT CCAAAACTGA CTCCTGTGTT
    TATGCAAAAA TGCCAAAGGG AAACTTGCCA GGGAAATGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290830
    Clone ID Related Accession (Same CDS sequence) XM_022423409.1
    Accession Version XM_022423409.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GAGACTTCCT CTTTAAATGT
    ACAGACAGTT CCGAAGTTGT CTTGGCCTCT GTCTGTGGAG AAAACCACAT ACTAATTCCA
    CTCCGTGAGA GAACAGGAAA AGCTTTGGGA GTTCTTGATT TTAACATTGG CAAGAGTAAA
    ATGTTATTGT ATCGAGAATT TAAAGACCTA CAGAAAATGA TGAAAGTGGT CCAAGCTGCC
    TGCTACGAAA TACTGGGCGA ATTATCTGGA GAAATAAAGA AAAACTATAT CCTAGAGATT
    GAAAATGTAG GAGAAGTCCA GCGGGCAGGA GTTCTCTTTT TCCGAATCAT GCTACAAGAG
    CTGCAAGAAA GCCTCCGATT GCTTACCTCC ATAGACTTTG TATCACTGTT GATCTATGAT
    TATCATATTC CAGCAGAGTC CGTATCTCTA CCAGACAGCA AGACCCAGGA GTTGGAAGCC
    AACATAAAGC TAGTACAGGA CATCCTGATA GGGGTTATTT TGTTCATCCA TCCAGAGTTG
    GAATTATCAA GTGAGTTTGG AAATTGGGAG AAGTGTAAAC TGTATGTTAG CAGATATTTA
    GTCGAGAATA TTTGTGACTT TGATCCAACT GCTAAGCATG TGGAAGTTAA TTTACAGCTC
    ATTGGTGAAT ATATCAGAGG TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATATTGTC
    ATCGAATACC TATACCACTG GGCACACATC TGCTTAGCTC TCATGGAGCT AAACAAAAAA
    CCAAGCAGTG CTATTTTACC CCCATTGCCC TCCAAAACTG ACTCCTGTGT TTATGCAAAA
    ATGCCAAAGG GAAACTTGCC AGGGAAATGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279117.1
    CDS220..4692
    Translation

    Target ORF information:

    RefSeq Version XM_022423409.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GAGACTTCCT CTTTAAATGT
    ACAGACAGTT CCGAAGTTGT CTTGGCCTCT GTCTGTGGAG AAAACCACAT ACTAATTCCA
    CTCCGTGAGA GAACAGGAAA AGCTTTGGGA GTTCTTGATT TTAACATTGG CAAGAGTAAA
    ATGTTATTGT ATCGAGAATT TAAAGACCTA CAGAAAATGA TGAAAGTGGT CCAAGCTGCC
    TGCTACGAAA TACTGGGCGA ATTATCTGGA GAAATAAAGA AAAACTATAT CCTAGAGATT
    GAAAATGTAG GAGAAGTCCA GCGGGCAGGA GTTCTCTTTT TCCGAATCAT GCTACAAGAG
    CTGCAAGAAA GCCTCCGATT GCTTACCTCC ATAGACTTTG TATCACTGTT GATCTATGAT
    TATCATATTC CAGCAGAGTC CGTATCTCTA CCAGACAGCA AGACCCAGGA GTTGGAAGCC
    AACATAAAGC TAGTACAGGA CATCCTGATA GGGGTTATTT TGTTCATCCA TCCAGAGTTG
    GAATTATCAA GTGAGTTTGG AAATTGGGAG AAGTGTAAAC TGTATGTTAG CAGATATTTA
    GTCGAGAATA TTTGTGACTT TGATCCAACT GCTAAGCATG TGGAAGTTAA TTTACAGCTC
    ATTGGTGAAT ATATCAGAGG TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATATTGTC
    ATCGAATACC TATACCACTG GGCACACATC TGCTTAGCTC TCATGGAGCT AAACAAAAAA
    CCAAGCAGTG CTATTTTACC CCCATTGCCC TCCAAAACTG ACTCCTGTGT TTATGCAAAA
    ATGCCAAAGG GAAACTTGCC AGGGAAATGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290831
    Clone ID Related Accession (Same CDS sequence) XM_022423411.1
    Accession Version XM_022423411.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGCAGA GTCCGTATCT CTACCAGACA GCAAGACCCA GGAGTTGGAA
    GCCAACATAA AGCTAGTACA GGACATCCTG ATAGGGGTTA TTTTGTTCAT CCATCCAGAG
    TTGGAATTAT CAAGTGAGTT TGGAAATTGG GAGAAGTGTA AACTGTATGT TAGCAGATAT
    TTAGTCGAGA ATATTTGTGA CTTTGATCCA ACTGCTAAGC ATGTGGAAGT TAATTTACAG
    CTCATTGGTG AATATATCAG AGGTCATTCT CGAATTGAAG TATGGAAATT TGGTAATATT
    GTCATCGAAT ACCTATACCA CTGGGCACAC ATCTGCTTAG CTCTCATGGA GCTAAACAAA
    AAACCAAGCA GTGCTATTTT ACCCCCATTG CCCTCCAAAA CTGACTCCTG TGTTTATGCA
    AAAATGCCAA AGGGAAACTT GCCAGGGAAA TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279119.1
    CDS220..4575
    Translation

    Target ORF information:

    RefSeq Version XM_022423411.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423411.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGCAGA GTCCGTATCT CTACCAGACA GCAAGACCCA GGAGTTGGAA
    GCCAACATAA AGCTAGTACA GGACATCCTG ATAGGGGTTA TTTTGTTCAT CCATCCAGAG
    TTGGAATTAT CAAGTGAGTT TGGAAATTGG GAGAAGTGTA AACTGTATGT TAGCAGATAT
    TTAGTCGAGA ATATTTGTGA CTTTGATCCA ACTGCTAAGC ATGTGGAAGT TAATTTACAG
    CTCATTGGTG AATATATCAG AGGTCATTCT CGAATTGAAG TATGGAAATT TGGTAATATT
    GTCATCGAAT ACCTATACCA CTGGGCACAC ATCTGCTTAG CTCTCATGGA GCTAAACAAA
    AAACCAAGCA GTGCTATTTT ACCCCCATTG CCCTCCAAAA CTGACTCCTG TGTTTATGCA
    AAAATGCCAA AGGGAAACTT GCCAGGGAAA TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290832
    Clone ID Related Accession (Same CDS sequence) XM_022423412.1
    Accession Version XM_022423412.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGTC CGTATCTCTA CCAGACAGCA AGACCCAGGA GTTGGAAGCC
    AACATAAAGC TAGTACAGGA CATCCTGATA GGGGTTATTT TGTTCATCCA TCCAGAGTTG
    GAATTATCAA GTGAGTTTGG AAATTGGGAG AAGTGTAAAC TGTATGTTAG CAGATATTTA
    GTCGAGAATA TTTGTGACTT TGATCCAACT GCTAAGCATG TGGAAGTTAA TTTACAGCTC
    ATTGGTGAAT ATATCAGAGG TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATATTGTC
    ATCGAATACC TATACCACTG GGCACACATC TGCTTAGCTC TCATGGAGCT AAACAAAAAA
    CCAAGCAGTG CTATTTTACC CCCATTGCCC TCCAAAACTG ACTCCTGTGT TTATGCAAAA
    ATGCCAAAGG GAAACTTGCC AGGGAAATGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279120.1
    CDS220..4572
    Translation

    Target ORF information:

    RefSeq Version XM_022423412.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGTC CGTATCTCTA CCAGACAGCA AGACCCAGGA GTTGGAAGCC
    AACATAAAGC TAGTACAGGA CATCCTGATA GGGGTTATTT TGTTCATCCA TCCAGAGTTG
    GAATTATCAA GTGAGTTTGG AAATTGGGAG AAGTGTAAAC TGTATGTTAG CAGATATTTA
    GTCGAGAATA TTTGTGACTT TGATCCAACT GCTAAGCATG TGGAAGTTAA TTTACAGCTC
    ATTGGTGAAT ATATCAGAGG TCATTCTCGA ATTGAAGTAT GGAAATTTGG TAATATTGTC
    ATCGAATACC TATACCACTG GGCACACATC TGCTTAGCTC TCATGGAGCT AAACAAAAAA
    CCAAGCAGTG CTATTTTACC CCCATTGCCC TCCAAAACTG ACTCCTGTGT TTATGCAAAA
    ATGCCAAAGG GAAACTTGCC AGGGAAATGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290833
    Clone ID Related Accession (Same CDS sequence) XM_022423413.1
    Accession Version XM_022423413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGATT CAGACTATGT TTTACGCAAT ATGATGGTTA CCAGACACCT GGGTCTAACA
    GAAATCCACA GGAACCCCCC TATAATTTTC AGGAAGACAT GCATCTTCAG AGACTTCCTC
    TTTAAATGTA CAGACAGTTC CGAAGTTGTC TTGGCCTCTG TCTGTGGAGA AAACCACATA
    CTAATTCCAC TCCGTGAGAG AACAGGAAAA GCTTTGGGAG TTCTTGATTT TAACATTGGC
    AAGAGTAAAA TGTTATTGTA TCGAGAATTT AAAGACCTAC AGAAAATGAT GAAAGTGGTC
    CAAGCTGCCT GCTACGAAAT ACTGGGCGAA TTATCTGGAG AAATAAAGAA AAACTATATC
    CTAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279121.1
    CDS220..4560
    Translation

    Target ORF information:

    RefSeq Version XM_022423413.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGATT CAGACTATGT TTTACGCAAT ATGATGGTTA CCAGACACCT GGGTCTAACA
    GAAATCCACA GGAACCCCCC TATAATTTTC AGGAAGACAT GCATCTTCAG AGACTTCCTC
    TTTAAATGTA CAGACAGTTC CGAAGTTGTC TTGGCCTCTG TCTGTGGAGA AAACCACATA
    CTAATTCCAC TCCGTGAGAG AACAGGAAAA GCTTTGGGAG TTCTTGATTT TAACATTGGC
    AAGAGTAAAA TGTTATTGTA TCGAGAATTT AAAGACCTAC AGAAAATGAT GAAAGTGGTC
    CAAGCTGCCT GCTACGAAAT ACTGGGCGAA TTATCTGGAG AAATAAAGAA AAACTATATC
    CTAGAGTCCG TATCTCTACC AGACAGCAAG ACCCAGGAGT TGGAAGCCAA CATAAAGCTA
    GTACAGGACA TCCTGATAGG GGTTATTTTG TTCATCCATC CAGAGTTGGA ATTATCAAGT
    GAGTTTGGAA ATTGGGAGAA GTGTAAACTG TATGTTAGCA GATATTTAGT CGAGAATATT
    TGTGACTTTG ATCCAACTGC TAAGCATGTG GAAGTTAATT TACAGCTCAT TGGTGAATAT
    ATCAGAGGTC ATTCTCGAAT TGAAGTATGG AAATTTGGTA ATATTGTCAT CGAATACCTA
    TACCACTGGG CACACATCTG CTTAGCTCTC ATGGAGCTAA ACAAAAAACC AAGCAGTGCT
    ATTTTACCCC CATTGCCCTC CAAAACTGAC TCCTGTGTTT ATGCAAAAAT GCCAAAGGGA
    AACTTGCCAG GGAAATGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290834
    Clone ID Related Accession (Same CDS sequence) XM_022423414.1
    Accession Version XM_022423414.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAAGTAGGA TTTTTGGATC GGCAGAGGAC ATTGGCTTTG
    TTGGAAACAT TCTATGACCA AAGTTCAAAA ATGCTTAGAA GTTTGCTACG AAACCCAAGA
    CAATGGCCAT TCATTGAATT TGAAGAGATA GATTTGCCTG AGTTCTGGGG AGACATGGAT
    AATCAGAAAC ATATTTATGA AGACTTTGAC AAAGTGCTCT TAGAGATGAA TGCACAACTT
    TCTGAAAAAT ATGCTAGCAA AACCCAAAGC AAATTGTTAG AAACTCCAGA AGATCAACAT
    TATCATGATG AACATAGTGA ATCAACACTA CCAGAACAGC AGAGAGGGAC AACTGCAGAA
    CAAGAACGAA ACAGAATTTT AAGCAAGGAA CAAGAACAAG GCAGAGAGTT AACTGGAGAA
    AAAGAACTAG ACAAAGACTC AGTAACAGAA CAAGAAACCC ACACAGGGTC AACTCCAGAA
    CAGGGATCAA GTAGAGAGTT AATAATAGAA CAAAGACTAC ATAGTGACTC AGTAATAGAA
    CAAGAGCCAC CACAAAGCAT GTCTTCTTCA GAACAAGGAG TCAACATGGG AACAACTGCA
    GAACAAGGAC TCTACAGAGA ATCAATAGCA GCACAAGGAC AACACAAACG GTCAGACACA
    GAACAAGGAC CACACAGAGG GTCAACCTCA GAACAAGGAT CACGCAGACA GTCAGTAATA
    GAACAAGAAC CACTCAAAGG GTCAACTGCA GAACAAGAGC CATCCAGAGA GATATTCCCA
    GAAAGACAAC AGGATTCAAC CTTACAATCA GGAAGAGATA GTATTTTCAG AGAAAGTAAA
    ATATTTAGGA ATGCTACTTC CTTTGAGTAC ACTGAAATCT CCCCACAGAA AGAGAGGACT
    CAGGAACAAA CCTATGAAAA AGAACTATTT GTGAGTTCTG AGCTGCAAGA AGAAGTCTCA
    ACATCACGAA GAAAAGATCA TCTTTCAGAA ACTACAAAAA AGGAAATTCA GAAAGATCCA
    TCCTGTGAAA AGTCCCAAAA AATAGAAGGA AAGTCATGGT CAGGTGAACT TCTTACTTGT
    AACTGGAAGA TGAATTATTT CAAATGTGAA GATGAGGAGC AAGCCAACTT ACTCTACAGT
    GATAACAGGT TCACAGACCT ACACTCAATT ATCAGAAATA TTCAGTCTTA CAAGGAAGTA
    AAAGGTAGAA GTGCTTTCAA TGGAGTTTCC TTCAATCTGC TCCAGTTTGT GCAACTTTTG
    GAGACATTTG TTGGTGAAGA TGTCCCCTTG AGTGTCAGTG AGACTCTCAC AACCTTTTTT
    AAGAGGGGCT ATGTTGAAAC AAAAGAAGAG AAAATAAGTG GCTTAGAACA GACTAGACAA
    AATGCTTCCA GAGTCCGACA GGAACTTCTC CTAGAAGCCC TGTTTCAGAA GTGGGACAGT
    GATGGCTCAG GCTTCTTGGA TCTGAAGGAA ATTGATGAAC TTTTGTACAC ATACAAGGAG
    GGAATGGAAA AAGAATCCAT GAAGAAAGCT AAACTACACA TCCAATTTCC AAAGCCACAA
    CCTGATCAAG AAGTGAGACT GTCTTCAAAA CAATTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGATCATGTT CTGGAAAGTA TTGTGGAGTT TCTGGTGAAT
    ACTTTAGAAA GGAGCCATAC TGAGAATCTG AGGAATTGTG CCAGACGAAA GTGGCTATAC
    CAAATCCAAC ATGCTGCAGA GACAAGTGGA GTATCCCTGG AGCCTGTGTA CACTGAAACC
    TTTAAGGCCC TCACCCAGGA TGCTGAAGCC CATGGAAATA AGAAGATCTG TGCTCATATT
    TCCCTCTTAG AAGAAAACCT ACTACTGCCT GATAGAGGGG AAGTTGTATT AAGGAACGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTA CTGAACAAAG TGCTCTACAG GGACATGAAG
    GGAATCAGCT TTACAGTAGT GGATGAAGGT AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACG TCTTTTTCTG GAACCATTCC CTCAAGAAGA GTGATCAAAA TGGATCATTC
    CTGGCCCTGC CTCTTCAGGA TGCATACATG AGGATCTTTG GGGTCATGGC TGTGGACACC
    CTAAGAGATA AAAAAAAAAT AAACATCTTT GTACCTCATG AGATCAGATT CTATCAGGGT
    GTTGCTAATG TCTTTAGCAC TGCCTATCAC TATGTCCACA GCAGGGAGCA CATCCTGCAT
    GTTGTGATTA CTGGCATTGG CTGGCTCTGT GACATCACAT CTGACATCAC CGCCATCACT
    ACATACTTCA TTGAGCCTGG CCTAATGCAG GCTTCTTCCT CTCTGAGTGG TTGGACCAAG
    CTGATAAGGC CCTGGCCAAA GAGCAGAATC ACAGAGCTCT CTGGCAGAAT GAACAGACTG
    TGGAGGTGGA GAACGCATAG TCCCGGAGAC TTCCTCTTTA AATGTACAGA CAGTTCCGAA
    GTTGTCTTGG CCTCTGTCTG TGGAGAAAAC CACATACTAA TTCCACTCCG TGAGAGAACA
    GGAAAAGCTT TGGGAGTTCT TGATTTTAAC ATTGGCAAGA GTAAAATGTT ATTGTATCGA
    GAATTTAAAG ACCTACAGAA AATGATGAAA GTGGTCCAAG CTGCCTGCTA CGAAATACTG
    GGCGAATTAT CTGGAGAAAT AAAGAAAAAC TATATCCTAG AGATTGAAAA TGTAGGAGAA
    GTCCAGCGGG CAGGAGTTCT CTTTTTCCGA ATCATGCTAC AAGAGCTGCA AGAAAGCCTC
    CGATTGCTTA CCTCCATAGA CTTTGTATCA CTGTTGATCT ATGATTATCA TATTCCAGCA
    GAGTCCGTAT CTCTACCAGA CAGCAAGACC CAGGAGTTGG AAGCCAACAT AAAGCTAGTA
    CAGGACATCC TGATAGGGGT TATTTTGTTC ATCCATCCAG AGTTGGAATT ATCAAGTGAG
    TTTGGAAATT GGGAGAAGTG TAAACTGTAT GTTAGCAGAT ATTTAGTCGA GAATATTTGT
    GACTTTGATC CAACTGCTAA GCATGTGGAA GTTAATTTAC AGCTCATTGG TGAATATATC
    AGAGGTCATT CTCGAATTGA AGTATGGAAA TTTGGTAATA TTGTCATCGA ATACCTATAC
    CACTGGGCAC ACATCTGCTT AGCTCTCATG GAGCTAAACA AAAAACCAAG CAGTGCTATT
    TTACCCCCAT TGCCCTCCAA AACTGACTCC TGTGTTTATG CAAAAATGCC AAAGGGAAAC
    TTGCCAGGGA AATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279122.1
    CDS220..4557
    Translation

    Target ORF information:

    RefSeq Version XM_022423414.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423414.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAAGTAGGA TTTTTGGATC GGCAGAGGAC ATTGGCTTTG
    TTGGAAACAT TCTATGACCA AAGTTCAAAA ATGCTTAGAA GTTTGCTACG AAACCCAAGA
    CAATGGCCAT TCATTGAATT TGAAGAGATA GATTTGCCTG AGTTCTGGGG AGACATGGAT
    AATCAGAAAC ATATTTATGA AGACTTTGAC AAAGTGCTCT TAGAGATGAA TGCACAACTT
    TCTGAAAAAT ATGCTAGCAA AACCCAAAGC AAATTGTTAG AAACTCCAGA AGATCAACAT
    TATCATGATG AACATAGTGA ATCAACACTA CCAGAACAGC AGAGAGGGAC AACTGCAGAA
    CAAGAACGAA ACAGAATTTT AAGCAAGGAA CAAGAACAAG GCAGAGAGTT AACTGGAGAA
    AAAGAACTAG ACAAAGACTC AGTAACAGAA CAAGAAACCC ACACAGGGTC AACTCCAGAA
    CAGGGATCAA GTAGAGAGTT AATAATAGAA CAAAGACTAC ATAGTGACTC AGTAATAGAA
    CAAGAGCCAC CACAAAGCAT GTCTTCTTCA GAACAAGGAG TCAACATGGG AACAACTGCA
    GAACAAGGAC TCTACAGAGA ATCAATAGCA GCACAAGGAC AACACAAACG GTCAGACACA
    GAACAAGGAC CACACAGAGG GTCAACCTCA GAACAAGGAT CACGCAGACA GTCAGTAATA
    GAACAAGAAC CACTCAAAGG GTCAACTGCA GAACAAGAGC CATCCAGAGA GATATTCCCA
    GAAAGACAAC AGGATTCAAC CTTACAATCA GGAAGAGATA GTATTTTCAG AGAAAGTAAA
    ATATTTAGGA ATGCTACTTC CTTTGAGTAC ACTGAAATCT CCCCACAGAA AGAGAGGACT
    CAGGAACAAA CCTATGAAAA AGAACTATTT GTGAGTTCTG AGCTGCAAGA AGAAGTCTCA
    ACATCACGAA GAAAAGATCA TCTTTCAGAA ACTACAAAAA AGGAAATTCA GAAAGATCCA
    TCCTGTGAAA AGTCCCAAAA AATAGAAGGA AAGTCATGGT CAGGTGAACT TCTTACTTGT
    AACTGGAAGA TGAATTATTT CAAATGTGAA GATGAGGAGC AAGCCAACTT ACTCTACAGT
    GATAACAGGT TCACAGACCT ACACTCAATT ATCAGAAATA TTCAGTCTTA CAAGGAAGTA
    AAAGGTAGAA GTGCTTTCAA TGGAGTTTCC TTCAATCTGC TCCAGTTTGT GCAACTTTTG
    GAGACATTTG TTGGTGAAGA TGTCCCCTTG AGTGTCAGTG AGACTCTCAC AACCTTTTTT
    AAGAGGGGCT ATGTTGAAAC AAAAGAAGAG AAAATAAGTG GCTTAGAACA GACTAGACAA
    AATGCTTCCA GAGTCCGACA GGAACTTCTC CTAGAAGCCC TGTTTCAGAA GTGGGACAGT
    GATGGCTCAG GCTTCTTGGA TCTGAAGGAA ATTGATGAAC TTTTGTACAC ATACAAGGAG
    GGAATGGAAA AAGAATCCAT GAAGAAAGCT AAACTACACA TCCAATTTCC AAAGCCACAA
    CCTGATCAAG AAGTGAGACT GTCTTCAAAA CAATTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGATCATGTT CTGGAAAGTA TTGTGGAGTT TCTGGTGAAT
    ACTTTAGAAA GGAGCCATAC TGAGAATCTG AGGAATTGTG CCAGACGAAA GTGGCTATAC
    CAAATCCAAC ATGCTGCAGA GACAAGTGGA GTATCCCTGG AGCCTGTGTA CACTGAAACC
    TTTAAGGCCC TCACCCAGGA TGCTGAAGCC CATGGAAATA AGAAGATCTG TGCTCATATT
    TCCCTCTTAG AAGAAAACCT ACTACTGCCT GATAGAGGGG AAGTTGTATT AAGGAACGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTA CTGAACAAAG TGCTCTACAG GGACATGAAG
    GGAATCAGCT TTACAGTAGT GGATGAAGGT AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACG TCTTTTTCTG GAACCATTCC CTCAAGAAGA GTGATCAAAA TGGATCATTC
    CTGGCCCTGC CTCTTCAGGA TGCATACATG AGGATCTTTG GGGTCATGGC TGTGGACACC
    CTAAGAGATA AAAAAAAAAT AAACATCTTT GTACCTCATG AGATCAGATT CTATCAGGGT
    GTTGCTAATG TCTTTAGCAC TGCCTATCAC TATGTCCACA GCAGGGAGCA CATCCTGCAT
    GTTGTGATTA CTGGCATTGG CTGGCTCTGT GACATCACAT CTGACATCAC CGCCATCACT
    ACATACTTCA TTGAGCCTGG CCTAATGCAG GCTTCTTCCT CTCTGAGTGG TTGGACCAAG
    CTGATAAGGC CCTGGCCAAA GAGCAGAATC ACAGAGCTCT CTGGCAGAAT GAACAGACTG
    TGGAGGTGGA GAACGCATAG TCCCGGAGAC TTCCTCTTTA AATGTACAGA CAGTTCCGAA
    GTTGTCTTGG CCTCTGTCTG TGGAGAAAAC CACATACTAA TTCCACTCCG TGAGAGAACA
    GGAAAAGCTT TGGGAGTTCT TGATTTTAAC ATTGGCAAGA GTAAAATGTT ATTGTATCGA
    GAATTTAAAG ACCTACAGAA AATGATGAAA GTGGTCCAAG CTGCCTGCTA CGAAATACTG
    GGCGAATTAT CTGGAGAAAT AAAGAAAAAC TATATCCTAG AGATTGAAAA TGTAGGAGAA
    GTCCAGCGGG CAGGAGTTCT CTTTTTCCGA ATCATGCTAC AAGAGCTGCA AGAAAGCCTC
    CGATTGCTTA CCTCCATAGA CTTTGTATCA CTGTTGATCT ATGATTATCA TATTCCAGCA
    GAGTCCGTAT CTCTACCAGA CAGCAAGACC CAGGAGTTGG AAGCCAACAT AAAGCTAGTA
    CAGGACATCC TGATAGGGGT TATTTTGTTC ATCCATCCAG AGTTGGAATT ATCAAGTGAG
    TTTGGAAATT GGGAGAAGTG TAAACTGTAT GTTAGCAGAT ATTTAGTCGA GAATATTTGT
    GACTTTGATC CAACTGCTAA GCATGTGGAA GTTAATTTAC AGCTCATTGG TGAATATATC
    AGAGGTCATT CTCGAATTGA AGTATGGAAA TTTGGTAATA TTGTCATCGA ATACCTATAC
    CACTGGGCAC ACATCTGCTT AGCTCTCATG GAGCTAAACA AAAAACCAAG CAGTGCTATT
    TTACCCCCAT TGCCCTCCAA AACTGACTCC TGTGTTTATG CAAAAATGCC AAAGGGAAAC
    TTGCCAGGGA AATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290835
    Clone ID Related Accession (Same CDS sequence) XM_022423415.1
    Accession Version XM_022423415.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GGCTGCCTGG GTGGCGCAGC
    GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279123.1
    CDS220..4545
    Translation

    Target ORF information:

    RefSeq Version XM_022423415.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGTGGAGAAC GCATAGTCCC
    GGAGACTTCC TCTTTAAATG TACAGACAGT TCCGAAGTTG TCTTGGCCTC TGTCTGTGGA
    GAAAACCACA TACTAATTCC ACTCCGTGAG AGAACAGGAA AAGCTTTGGG AGTTCTTGAT
    TTTAACATTG GCAAGAGTAA AATGTTATTG TATCGAGAAT TTAAAGACCT ACAGAAAATG
    ATGAAAGTGG TCCAAGCTGC CTGCTACGAA ATACTGGGCG AATTATCTGG AGAAATAAAG
    AAAAACTATA TCCTAGAGAT TGAAAATGTA GGAGAAGTCC AGCGGGCAGG AGTTCTCTTT
    TTCCGAATCA TGCTACAAGA GCTGCAAGAA AGCCTCCGAT TGCTTACCTC CATAGACTTT
    GTATCACTGT TGATCTATGA TTATCATATT CCAGCAGAGT CCGTATCTCT ACCAGACAGC
    AAGACCCAGG AGTTGGAAGC CAACATAAAG CTAGTACAGG ACATCCTGAT AGGGGTTATT
    TTGTTCATCC ATCCAGAGTT GGAATTATCA AGTGAGTTTG GAAATTGGGA GAAGTGTAAA
    CTGTATGTTA GCAGATATTT AGTCGAGAAT ATTTGTGACT TTGATCCAAC TGCTAAGCAT
    GTGGAAGTTA ATTTACAGCT CATTGGTGAA TATATCAGAG GGCTGCCTGG GTGGCGCAGC
    GGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290836
    Clone ID Related Accession (Same CDS sequence) XM_022423416.1
    Accession Version XM_022423416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGGTAGGA TTTTTGGATC GGCAGAGGAC ATTGGCTTTG
    TTGGAAACAT TCTATGACCA AAGTTCAAAA ATGCTTAGAA GTTTGCTACG AAACCCAAGA
    CAATGGCCAT TCATTGAATT TGAAGAGATA GATTTGCCTG AGTTCTGGGG AGACATGGAT
    AATCAGAAAC ATATTTATGA AGACTTTGAC AAAGTGCTCT TAGAGATGAA TGCACAACTT
    TCTGAAAAAT ATGCTAGCAA AACCCAAAGC AAATTGTTAG AAACTCCAGA AGATCAACAT
    TATCATGATG AACATAGTGA ATCAACACTA CCAGAACAGC AGAGAGGGAC AACTGCAGAA
    CAAGAACGAA ACAGAATTTT AAGCAAGGAA CAAGAACAAG GCAGAGAGTT AACTGGAGAA
    AAAGAACTAG ACAAAGACTC AGTAACAGAA CAAGAAACCC ACACAGGGTC AACTCCAGAA
    CAGGGATCAA GTAGAGAGTT AATAATAGAA CAAAGACTAC ATAGTGACTC AGTAATAGAA
    CAAGAGCCAC CACAAAGCAT GTCTTCTTCA GAACAAGGAG TCAACATGGG AACAACTGCA
    GAACAAGGAC TCTACAGAGA ATCAATAGCA GCACAAGGAC AACACAAACG GTCAGACACA
    GAACAAGGAC CACACAGAGG GTCAACCTCA GAACAAGGAT CACGCAGACA GTCAGTAATA
    GAACAAGAAC CACTCAAAGG GTCAACTGCA GAACAAGAGC CATCCAGAGA GATATTCCCA
    GAAAGACAAC AGGATTCAAC CTTACAATCA GGAAGAGATA GTATTTTCAG AGAAAGTAAA
    ATATTTAGGA ATGCTACTTC CTTTGAGTAC ACTGAAATCT CCCCACAGAA AGAGAGGACT
    CAGGAACAAA CCTATGAAAA AGAACTATTT GTGAGTTCTG AGCTGCAAGA AGAAGTCTCA
    ACATCACGAA GAAAAGATCA TCTTTCAGAA ACTACAAAAA AGGAAATTCA GAAAGATCCA
    TCCTGTGAAA AGTCCCAAAA AATAGAAGGA AAGTCATGGT CAGGTGAACT TCTTACTTGT
    AACTGGAAGA TGAATTATTT CAAATGTGAA GATGAGGAGC AAGCCAACTT ACTCTACAGT
    GATAACAGGT TCACAGACCT ACACTCAATT ATCAGAAATA TTCAGTCTTA CAAGGAAGTA
    AAAGGTAGAA GTGCTTTCAA TGGAGTTTCC TTCAATCTGC TCCAGTTTGT GCAACTTTTG
    GAGACATTTG TTGGTGAAGA TGTCCCCTTG AGTGTCAGTG AGACTCTCAC AACCTTTTTT
    AAGAGGGGCT ATGTTGAAAC AAAAGAAGAG AAAATAAGTG GCTTAGAACA GACTAGACAA
    AATGCTTCCA GAGTCCGACA GGAACTTCTC CTAGAAGCCC TGTTTCAGAA GTGGGACAGT
    GATGGCTCAG GCTTCTTGGA TCTGAAGGAA ATTGATGAAC TTTTGTACAC ATACAAGGAG
    GGAATGGAAA AAGAATCCAT GAAGAAAGCT AAACTACACA TCCAATTTCC AAAGCCACAA
    CCTGATCAAG AAGTGAGACT GTCTTCAAAA CAATTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGATCATGTT CTGGAAAGTA TTGTGGAGTT TCTGGTGAAT
    ACTTTAGAAA GGAGCCATAC TGAGAATCTG AGGAATTGTG CCAGACGAAA GTGGCTATAC
    CAAATCCAAC ATGCTGCAGA GACAAGTGGA GTATCCCTGG AGCCTGTGTA CACTGAAACC
    TTTAAGGCCC TCACCCAGGA TGCTGAAGCC CATGGAAATA AGAAGATCTG TGCTCATATT
    TCCCTCTTAG AAGAAAACCT ACTACTGCCT GATAGAGGGG AAGTTGTATT AAGGAACGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTA CTGAACAAAG TGCTCTACAG GGACATGAAG
    GGAATCAGCT TTACAGTAGT GGATGAAGGT AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACG TCTTTTTCTG GAACCATTCC CTCAAGAAGA GTGATCAAAA TGGATCATTC
    CTGGCCCTGC CTCTTCAGGA TGCATACATG AGGATCTTTG GGGTCATGGC TGTGGACACC
    CTAAGAGATA AAAAAAAAAT AAACATCTTT GTACCTCATG AGATCAGATT CTATCAGGGT
    GTTGCTAATG TCTTTAGCAC TGCCTATCAC TATGTCCACA GCAGGGAGCA CATCCTGCAT
    GTTGTGATTA CTGGCATTGG CTGGCTCTGT GACATCACAT CTGACATCAC CGCCATCACT
    ACATACTTCA TTGAGCCTGG CCTAATGCAG GCTTCTTCCT CTCTGAGTGG TTGGACCAAG
    CTGATAAGGC CCTGGCCAAA GAGCAGAATC ACAGAGCTCT CTGGCAGAAT GAACAGACTG
    TGGAGGTGGA GAACGCATAG TCCCGGAGAC TTCCTCTTTA AATGTACAGA CAGTTCCGAA
    GTTGTCTTGG CCTCTGTCTG TGGAGAAAAC CACATACTAA TTCCACTCCG TGAGAGAACA
    GGAAAAGCTT TGGGAGTTCT TGATTTTAAC ATTGGCAAGA GTAAAATGTT ATTGTATCGA
    GAATTTAAAG ACCTACAGAA AATGATGAAA GTGGTCCAAG CTGCCTGCTA CGAAATACTG
    GGCGAATTAT CTGGAGAAAT AAAGAAAAAC TATATCCTAG AGATTGAAAA TGTAGGAGAA
    GTCCAGCGGG CAGGAGTTCT CTTTTTCCGA ATCATGCTAC AAGAGCTGCA AGAAAGCCTC
    CGATTGCTTA CCTCCATAGA CTTTGTATCA CTGTTGATCT ATGATTATCA TATTCCAGCA
    GAGTCCGTAT CTCTACCAGA CAGCAAGACC CAGGAGTTGG AAGCCAACAT AAAGCTAGTA
    CAGGACATCC TGATAGGGGT TATTTTGTTC ATCCATCCAG AGTTGGAATT ATCAAGTGAG
    TTTGGAAATT GGGAGAAGTG TAAACTGTAT GTTAGCAGAT ATTTAGTCGA GAATATTTGT
    GACTTTGATC CAACTGCTAA GCATGTGGAA GTTAATTTAC AGCTCATTGG TGAATATATC
    AGAGGTCATT CTCGAATTGA AGTATGGAAA TTTGGTAATA TTGTCATCGA ATACCTATAC
    CACTGGGCAC ACATCTGCTT AGCTCTCATG GAGCTAAACA AAAAACCAAG CAGTGCTATT
    TTACCCCCAT TGCCCTCCAA AACTGACTCC TGTGTTTATG CAAAAATGCC AAAGGGAAAC
    TTGCCAGGGA AATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279124.1
    CDS220..4437
    Translation

    Target ORF information:

    RefSeq Version XM_022423416.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGGTAGGA TTTTTGGATC GGCAGAGGAC ATTGGCTTTG
    TTGGAAACAT TCTATGACCA AAGTTCAAAA ATGCTTAGAA GTTTGCTACG AAACCCAAGA
    CAATGGCCAT TCATTGAATT TGAAGAGATA GATTTGCCTG AGTTCTGGGG AGACATGGAT
    AATCAGAAAC ATATTTATGA AGACTTTGAC AAAGTGCTCT TAGAGATGAA TGCACAACTT
    TCTGAAAAAT ATGCTAGCAA AACCCAAAGC AAATTGTTAG AAACTCCAGA AGATCAACAT
    TATCATGATG AACATAGTGA ATCAACACTA CCAGAACAGC AGAGAGGGAC AACTGCAGAA
    CAAGAACGAA ACAGAATTTT AAGCAAGGAA CAAGAACAAG GCAGAGAGTT AACTGGAGAA
    AAAGAACTAG ACAAAGACTC AGTAACAGAA CAAGAAACCC ACACAGGGTC AACTCCAGAA
    CAGGGATCAA GTAGAGAGTT AATAATAGAA CAAAGACTAC ATAGTGACTC AGTAATAGAA
    CAAGAGCCAC CACAAAGCAT GTCTTCTTCA GAACAAGGAG TCAACATGGG AACAACTGCA
    GAACAAGGAC TCTACAGAGA ATCAATAGCA GCACAAGGAC AACACAAACG GTCAGACACA
    GAACAAGGAC CACACAGAGG GTCAACCTCA GAACAAGGAT CACGCAGACA GTCAGTAATA
    GAACAAGAAC CACTCAAAGG GTCAACTGCA GAACAAGAGC CATCCAGAGA GATATTCCCA
    GAAAGACAAC AGGATTCAAC CTTACAATCA GGAAGAGATA GTATTTTCAG AGAAAGTAAA
    ATATTTAGGA ATGCTACTTC CTTTGAGTAC ACTGAAATCT CCCCACAGAA AGAGAGGACT
    CAGGAACAAA CCTATGAAAA AGAACTATTT GTGAGTTCTG AGCTGCAAGA AGAAGTCTCA
    ACATCACGAA GAAAAGATCA TCTTTCAGAA ACTACAAAAA AGGAAATTCA GAAAGATCCA
    TCCTGTGAAA AGTCCCAAAA AATAGAAGGA AAGTCATGGT CAGGTGAACT TCTTACTTGT
    AACTGGAAGA TGAATTATTT CAAATGTGAA GATGAGGAGC AAGCCAACTT ACTCTACAGT
    GATAACAGGT TCACAGACCT ACACTCAATT ATCAGAAATA TTCAGTCTTA CAAGGAAGTA
    AAAGGTAGAA GTGCTTTCAA TGGAGTTTCC TTCAATCTGC TCCAGTTTGT GCAACTTTTG
    GAGACATTTG TTGGTGAAGA TGTCCCCTTG AGTGTCAGTG AGACTCTCAC AACCTTTTTT
    AAGAGGGGCT ATGTTGAAAC AAAAGAAGAG AAAATAAGTG GCTTAGAACA GACTAGACAA
    AATGCTTCCA GAGTCCGACA GGAACTTCTC CTAGAAGCCC TGTTTCAGAA GTGGGACAGT
    GATGGCTCAG GCTTCTTGGA TCTGAAGGAA ATTGATGAAC TTTTGTACAC ATACAAGGAG
    GGAATGGAAA AAGAATCCAT GAAGAAAGCT AAACTACACA TCCAATTTCC AAAGCCACAA
    CCTGATCAAG AAGTGAGACT GTCTTCAAAA CAATTTCAGA AATACATAGA ATTGGTTGTC
    TCTGAACTCA GGGGCAATGA AGATCATGTT CTGGAAAGTA TTGTGGAGTT TCTGGTGAAT
    ACTTTAGAAA GGAGCCATAC TGAGAATCTG AGGAATTGTG CCAGACGAAA GTGGCTATAC
    CAAATCCAAC ATGCTGCAGA GACAAGTGGA GTATCCCTGG AGCCTGTGTA CACTGAAACC
    TTTAAGGCCC TCACCCAGGA TGCTGAAGCC CATGGAAATA AGAAGATCTG TGCTCATATT
    TCCCTCTTAG AAGAAAACCT ACTACTGCCT GATAGAGGGG AAGTTGTATT AAGGAACGTA
    GCTTGTACTT TAGATGATGC TCCATTTGTA CTGAACAAAG TGCTCTACAG GGACATGAAG
    GGAATCAGCT TTACAGTAGT GGATGAAGGT AAGCCAATAC ACGTCCCCCA AGTTCAGCAC
    CATGGGAACG TCTTTTTCTG GAACCATTCC CTCAAGAAGA GTGATCAAAA TGGATCATTC
    CTGGCCCTGC CTCTTCAGGA TGCATACATG AGGATCTTTG GGGTCATGGC TGTGGACACC
    CTAAGAGATA AAAAAAAAAT AAACATCTTT GTACCTCATG AGATCAGATT CTATCAGGGT
    GTTGCTAATG TCTTTAGCAC TGCCTATCAC TATGTCCACA GCAGGGAGCA CATCCTGCAT
    GTTGTGATTA CTGGCATTGG CTGGCTCTGT GACATCACAT CTGACATCAC CGCCATCACT
    ACATACTTCA TTGAGCCTGG CCTAATGCAG GCTTCTTCCT CTCTGAGTGG TTGGACCAAG
    CTGATAAGGC CCTGGCCAAA GAGCAGAATC ACAGAGCTCT CTGGCAGAAT GAACAGACTG
    TGGAGGTGGA GAACGCATAG TCCCGGAGAC TTCCTCTTTA AATGTACAGA CAGTTCCGAA
    GTTGTCTTGG CCTCTGTCTG TGGAGAAAAC CACATACTAA TTCCACTCCG TGAGAGAACA
    GGAAAAGCTT TGGGAGTTCT TGATTTTAAC ATTGGCAAGA GTAAAATGTT ATTGTATCGA
    GAATTTAAAG ACCTACAGAA AATGATGAAA GTGGTCCAAG CTGCCTGCTA CGAAATACTG
    GGCGAATTAT CTGGAGAAAT AAAGAAAAAC TATATCCTAG AGATTGAAAA TGTAGGAGAA
    GTCCAGCGGG CAGGAGTTCT CTTTTTCCGA ATCATGCTAC AAGAGCTGCA AGAAAGCCTC
    CGATTGCTTA CCTCCATAGA CTTTGTATCA CTGTTGATCT ATGATTATCA TATTCCAGCA
    GAGTCCGTAT CTCTACCAGA CAGCAAGACC CAGGAGTTGG AAGCCAACAT AAAGCTAGTA
    CAGGACATCC TGATAGGGGT TATTTTGTTC ATCCATCCAG AGTTGGAATT ATCAAGTGAG
    TTTGGAAATT GGGAGAAGTG TAAACTGTAT GTTAGCAGAT ATTTAGTCGA GAATATTTGT
    GACTTTGATC CAACTGCTAA GCATGTGGAA GTTAATTTAC AGCTCATTGG TGAATATATC
    AGAGGTCATT CTCGAATTGA AGTATGGAAA TTTGGTAATA TTGTCATCGA ATACCTATAC
    CACTGGGCAC ACATCTGCTT AGCTCTCATG GAGCTAAACA AAAAACCAAG CAGTGCTATT
    TTACCCCCAT TGCCCTCCAA AACTGACTCC TGTGTTTATG CAAAAATGCC AAAGGGAAAC
    TTGCCAGGGA AATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290837
    Clone ID Related Accession (Same CDS sequence) XM_022423419.1
    Accession Version XM_022423419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGATT CAGACTATGT TTTACGCAAT ATGATGGTTA CCAGACACCT GGGTCTAACA
    GAAATCCACA GGAACCCCCC TATAATTTTC AGGAAGACAT GCATCTTCAG GTCATTCTCG
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279127.1
    CDS220..3885
    Translation

    Target ORF information:

    RefSeq Version XM_022423419.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGATT CAGACTATGT TTTACGCAAT ATGATGGTTA CCAGACACCT GGGTCTAACA
    GAAATCCACA GGAACCCCCC TATAATTTTC AGGAAGACAT GCATCTTCAG GTCATTCTCG
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290838
    Clone ID Related Accession (Same CDS sequence) XM_022423420.1
    Accession Version XM_022423420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGATT CAGACTATGT TTTACGCAAT ATGATGGTTA CCAGACACCT GGGTCTAACA
    GAAATCCACA GGAACCCCCC TATAATTTTC AGGAAGACAT GCATCTTCAG GATTTCCTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279128.1
    CDS220..3882
    Translation

    Target ORF information:

    RefSeq Version XM_022423420.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGATT CAGACTATGT TTTACGCAAT ATGATGGTTA CCAGACACCT GGGTCTAACA
    GAAATCCACA GGAACCCCCC TATAATTTTC AGGAAGACAT GCATCTTCAG GATTTCCTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290839
    Clone ID Related Accession (Same CDS sequence) XM_022423421.1
    Accession Version XM_022423421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGCAATACAG ACTTTCTGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279129.1
    CDS220..3882
    Translation

    Target ORF information:

    RefSeq Version XM_022423421.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GGCAATACAG ACTTTCTGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290840
    Clone ID Related Accession (Same CDS sequence) XM_022423422.1
    Accession Version XM_022423422.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279130.1
    CDS220..3867
    Translation

    Target ORF information:

    RefSeq Version XM_022423422.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423422.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGGCTT CTTCCTCTCT GAGTGGTTGG ACCAAGCTGA TAAGGCCCTG GCCAAAGAGC
    AGAATCACAG AGCTCTCTGG CAGAATGAAC AGACTGTGGA GAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290841
    Clone ID Related Accession (Same CDS sequence) XM_022423423.1
    Accession Version XM_022423423.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGAGAT TGAAAATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279131.1
    CDS220..3780
    Translation

    Target ORF information:

    RefSeq Version XM_022423423.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGAGAT TGAAAATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290842
    Clone ID Related Accession (Same CDS sequence) XM_022423424.1
    Accession Version XM_022423424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGAGAC TTCCTCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279132.1
    CDS220..3780
    Translation

    Target ORF information:

    RefSeq Version XM_022423424.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGGGTGTTG CTAATGTCTT TAGCACTGCC
    TATCACTATG TCCACAGCAG GGAGCACATC CTGCATGTTG TGATTACTGG CATTGGCTGG
    CTCTGTGACA TCACATCTGA CATCACCGCC ATCACTACAT ACTTCATTGA GCCTGGCCTA
    ATGCAGAGAC TTCCTCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290843
    Clone ID Related Accession (Same CDS sequence) XM_022423425.1
    Accession Version XM_022423425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279133.1
    CDS220..3618
    Translation

    Target ORF information:

    RefSeq Version XM_022423425.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGGATGCT
    GAAGCCCATG GAAATAAGAA GATCTGTGCT CATATTTCCC TCTTAGAAGA AAACCTACTA
    CTGCCTGATA GAGGGGAAGT TGTATTAAGG AACGTAGCTT GTACTTTAGA TGATGCTCCA
    TTTGTACTGA ACAAAGTGCT CTACAGGGAC ATGAAGGGAA TCAGCTTTAC AGTAGTGGAT
    GAAGGTAAGC CAATACACGT CCCCCAAGTT CAGCACCATG GGAACGTCTT TTTCTGGAAC
    CATTCCCTCA AGAAGAGTGA TCAAAATGGA TCATTCCTGG CCCTGCCTCT TCAGGATGCA
    TACATGAGGA TCTTTGGGGT CATGGCTGTG GACACCCTAA GAGATAAAAA AAAAATAAAC
    ATCTTTGTAC CTCATGAGAT CAGATTCTAT CAGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe290844
    Clone ID Related Accession (Same CDS sequence) XM_022423426.1
    Accession Version XM_022423426.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGCTTTAC
    AGTAGTGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022279134.1
    CDS220..3231
    Translation

    Target ORF information:

    RefSeq Version XM_022423426.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 5 (EFCAB5), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTGAGT CAGCATTTCA AGAGGAACCT AAACCTGCTC AGGAAAATGG AAAAGAAGAC 
    AAAGAAAACA AACCCGAAGA AACTGAAGTG AAAGAACCTA CTGAGAGTTC ACAGGGTGTA
    CCAGGCAGTT CTGCATTAGA CACCAGCAGC AATGTGGCCG AAACAGAAGC AGATGAAATC
    AAATCCCAAG AAATACACCT GGAAGGGCAG TCAAAAACTT CAGTTTGTTC AATAAAGGAC
    TCTAGCACTA GAGTCTCAAA GAATTTTGTG GTTAGAAAAC CTGCAAAAGT GATCTTCAAT
    TTAGATCCAA CTGCGCTGAA TTCAAAGCTA GAACAGCCAT GGAAGAAAAA TTTTTTTGAA
    AGATTGGAGG CAAAAGCCCA GAAAATGCAG CAGAAAATAA TAGATAAGGA TAACCTGAAT
    AAAGAACTAG AAAAAAAGGC TGAAAAAAAC CTCCCTAGGG ATAATTTGGC CAAAGAGTGG
    TTTAATGCTG AAAGCATAAC ATTGGACAGT CGTGCATATT TGCTGGACAA ACTTCTACCT
    ACCTTAGTTC CTGGCGTGGA AAAAATGTTA ATGCAAGTGG AAAAGAAGAA GCTTTGGGCA
    GAAGGTGATA TTACAACCAA GTTTAATCCA AATAATCATT TGGGGGAATA CTTAATGAGA
    AACAATCCTT ATTATATAAA AGATTCAGGA ATGTCTGGTT ATCAAAGAGT GATGAGGGAT
    GTCACAGAAG ACCTGAAGAT ACATGTTCCC AACACTGTTG GCAACAGAGT AACCAAGATG
    AAGGAGAGTA TTAAACAGAA ACGAGAACAA AGACAATTCA TAAAAGAAGT CAAAGTCAGG
    GTGACCAAAA CACGGAAGCA GGCTCTGCAG GAGCAATTCA GTGAATGGAT TCTAGACCCC
    AAAGGAACAA TTCCTATGGT TGTGATTCAG AATGTTCTTC ATGAATTTTT TCAAAATCCA
    GATTTACAGC TTGAAATATG TTGCAAACCA ATGGATATTG TTGACTCGAT GGGACCAAGA
    TTGAACAAAA CAGAATTTAT AGAATATGTC TCTTCATATG TTGCAGACTT TAAAAATGAA
    ATGTTCGAGA AACTCCTTAA GCACTTTTGT CATTGTGCAG ATGAATTCCG GGAGGTTATA
    AAAACAGACA TGCGGAGGCA GATATTTGCT GAACTCTTCC TGCATTGTGA CCATGGGAAG
    GTAGGATTTT TGGATCGGCA GAGGACATTG GCTTTGTTGG AAACATTCTA TGACCAAAGT
    TCAAAAATGC TTAGAAGTTT GCTACGAAAC CCAAGACAAT GGCCATTCAT TGAATTTGAA
    GAGATAGATT TGCCTGAGTT CTGGGGAGAC ATGGATAATC AGAAACATAT TTATGAAGAC
    TTTGACAAAG TGCTCTTAGA GATGAATGCA CAACTTTCTG AAAAATATGC TAGCAAAACC
    CAAAGCAAAT TGTTAGAAAC TCCAGAAGAT CAACATTATC ATGATGAACA TAGTGAATCA
    ACACTACCAG AACAGCAGAG AGGGACAACT GCAGAACAAG AACGAAACAG AATTTTAAGC
    AAGGAACAAG AACAAGGCAG AGAGTTAACT GGAGAAAAAG AACTAGACAA AGACTCAGTA
    ACAGAACAAG AAACCCACAC AGGGTCAACT CCAGAACAGG GATCAAGTAG AGAGTTAATA
    ATAGAACAAA GACTACATAG TGACTCAGTA ATAGAACAAG AGCCACCACA AAGCATGTCT
    TCTTCAGAAC AAGGAGTCAA CATGGGAACA ACTGCAGAAC AAGGACTCTA CAGAGAATCA
    ATAGCAGCAC AAGGACAACA CAAACGGTCA GACACAGAAC AAGGACCACA CAGAGGGTCA
    ACCTCAGAAC AAGGATCACG CAGACAGTCA GTAATAGAAC AAGAACCACT CAAAGGGTCA
    ACTGCAGAAC AAGAGCCATC CAGAGAGATA TTCCCAGAAA GACAACAGGA TTCAACCTTA
    CAATCAGGAA GAGATAGTAT TTTCAGAGAA AGTAAAATAT TTAGGAATGC TACTTCCTTT
    GAGTACACTG AAATCTCCCC ACAGAAAGAG AGGACTCAGG AACAAACCTA TGAAAAAGAA
    CTATTTGTGA GTTCTGAGCT GCAAGAAGAA GTCTCAACAT CACGAAGAAA AGATCATCTT
    TCAGAAACTA CAAAAAAGGA AATTCAGAAA GATCCATCCT GTGAAAAGTC CCAAAAAATA
    GAAGGAAAGT CATGGTCAGG TGAACTTCTT ACTTGTAACT GGAAGATGAA TTATTTCAAA
    TGTGAAGATG AGGAGCAAGC CAACTTACTC TACAGTGATA ACAGGTTCAC AGACCTACAC
    TCAATTATCA GAAATATTCA GTCTTACAAG GAAGTAAAAG GTAGAAGTGC TTTCAATGGA
    GTTTCCTTCA ATCTGCTCCA GTTTGTGCAA CTTTTGGAGA CATTTGTTGG TGAAGATGTC
    CCCTTGAGTG TCAGTGAGAC TCTCACAACC TTTTTTAAGA GGGGCTATGT TGAAACAAAA
    GAAGAGAAAA TAAGTGGCTT AGAACAGACT AGACAAAATG CTTCCAGAGT CCGACAGGAA
    CTTCTCCTAG AAGCCCTGTT TCAGAAGTGG GACAGTGATG GCTCAGGCTT CTTGGATCTG
    AAGGAAATTG ATGAACTTTT GTACACATAC AAGGAGGGAA TGGAAAAAGA ATCCATGAAG
    AAAGCTAAAC TACACATCCA ATTTCCAAAG CCACAACCTG ATCAAGAAGT GAGACTGTCT
    TCAAAACAAT TTCAGAAATA CATAGAATTG GTTGTCTCTG AACTCAGGGG CAATGAAGAT
    CATGTTCTGG AAAGTATTGT GGAGTTTCTG GTGAATACTT TAGAAAGGAG CCATACTGAG
    AATCTGAGGA ATTGTGCCAG ACGAAAGTGG CTATACCAAA TCCAACATGC TGCAGAGACA
    AGTGGAGTAT CCCTGGAGCC TGTGTACACT GAAACCTTTA AGGCCCTCAC CCAGCTTTAC
    AGTAGTGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.