EFCAB5 cDNA ORF clone, Gallus gallus(chicken)

The following EFCAB5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFCAB5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa145640 XM_025142051.1
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa145640 XM_025142050.1
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa17247 XM_015296106.2
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa17247 XM_025142049.1
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa17247 XM_015296105.1
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa17247 XM_025142048.1
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa17247 XM_015296104.1
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa17248 XM_004946638.3
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa17249 XM_015296107.2
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa17250 XM_004946639.3
Latest version!
Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa17250 XM_004946639.2 Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa17247 XM_015296106.1 Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa17248 XM_004946638.2 Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa17249 XM_015296107.1 Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa145640
    Clone ID Related Accession (Same CDS sequence) XM_025142050.1 , XM_025142051.1
    Accession Version XM_025142050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGTCTG AGGCAAAAAC CAAGCGTAAG GAAAGAGAAC AGGCAGAAAG GATGAAGTGC 
    CAGCAGAAGG AGATGAGAAA AGCAGCACTG GCAACTCAGT TCAAAGAATG GACAGCGGAT
    GTCAGTGGCA GAATTCCAGT TGCACTGATT CAGAGTGCAC TGAGGATTTT TCTGGATGTC
    TGTGCTTCTA TGCCCGTAGA CACAAGAAAA GCCTATGAGA GGAAGCTGGA AATCACAGAC
    ATGTGGGGGG AAAACGTAAA TGCTGATGGA TTCATAGAGT ATGTCCATTC CTACACGGAG
    AACTTTCCAA ATGATGCATT CCAGGAGGTC CTGAAGCACC TCGGTCAGTG TGCTGCTGAC
    TTCCAAGAAT CCATCAGGCA TAATACGTGC AGGCAGATGT TTATTGACCT CTTCCTACAC
    TGTGATCGAG GACAGGTTGG TGTCTTGGAT AGACCCCAAA TACTTGACCT GCTGGAGCAG
    TTCTATGAGA GGAGCTCTGT GTGTGCCAGG AGGAGATTCT GCAACCCCAA AGAGTGGCCA
    ATAGTCGAGC TGCAGGAGGT CGGTCTGGAG GATCTGTGGG GAGGCCTTGA GGACCAGCAA
    GGTTATGAGG AGCCTGGAGA AGGTTTGTTT TATTCTCAAG CAGAGATTTC TGAGAGAGAC
    GTTTCAGCAC GTGAAGCTGC AGCTGACGAC AGGGCGGGCA GTGATGGAAT GAGCATAATT
    GACAGAGGTC GTTTGGAACA AATGGATGTG AGTGAAACAG AGACTGGAGG AATTCCTACG
    GAAGAAAACC AAGCAGCAGA ATCAGGTGGT GCTGAGGATG CTGTGGCTGC AGAGGCCATG
    AAGGAACCTC CCGCAGGAGA AGAGGGTTTC AGAAGGCAGC ACAGCACCTG GTCTGGGCAG
    CAGACAAGCT CAGGGACAGC AGTGCAGGAC AAGGCCCATC TGCAGGAGCC AGCTGAAGAC
    CTCCATAACA CCATGGCAGG CATTGAGAGC CGCAGTGCTC CTCGTGCTGG GAGCGCCTTC
    GATGGGAGCT GCCTCAACCT GCCGCGCTTT GTTCAGCTCA TGGAGACGTT CCTCGGGGAA
    GGCATGGCTC TGCCTGCCGT GGAGGGGCTC ATTGGGTTCA TCAGAGAGGA ATACAAACAG
    ACCGAAGAGG AAAAAGTGAA ACAACTAGAA AAAGCACATC ACAGCTCCCG TTTGACCCGA
    CGGGAACTGC TTCTGGAAGC ACTGTTTGAA AAGTGGGACA TCAACGCGTC CGGGTTTCTG
    GAGATGAAAG AGGTGGAGGC TGCTCTGAGT GCATTCAAGG AAGGAATGGA AAAAGAGGCC
    GTGAGGATGG CAAAGAACCA CTTTTGCTCA CGTTACCCTC AGCTTGGCAG AGTGGGGAAG
    CTATCCCCAA AGGCATTCCA GGCTTTCCTC GAGTTGGTGG CTTCTGAGCT CACTGGGGAT
    GAAGATGAAG TCATTGATAA CATCGTGGAG TTTCTGACCA CAAGAGTGGA ACGAAGTCAC
    ACGGAGTGCT TGCAAAGCTT GGCGAGGAGG AGATGGCTGC ACAGCATCCA GCAGGCGGCC
    GAGAGGAGCG GAGCACGCAT GGAGCCGGTG TACGACGCGG TGCTCAAGGC CCTCCTCAAG
    GATGCAGAAG CCCACGGGGA TAACAAGAAG ATCAGTGCAT ATATTGCCCT TTTGGAAGAA
    AACCAGCTCT CACCAGAGCG GGGACAGACG CTCTTACGCT ATGTGACATG CACCACAGAT
    GATGCTCCGT ATGTTCTAAA CCAGATACTC TACAGAGACA TGAAAGGAGT AAGCTTTGCA
    GCTGTAGAAG AGGAAAAGCC AATCCATGTG CCAAGAGTTC AGCTGCATGG AAATATCCAC
    TTCTGGAACC AGGACCGCCC AGCTGAGCAG AGGAGAGGCT CATTCCTGGT ACTGCCATTG
    CAGGATGCTC ACTGGAGAGT CTTTGGCACC CTGGGACTTG ATACCCTTCG GGACCCAAGT
    GAGAAAACCA TCTTCCTGAC CCACGAGATC AGCTTCTACC AGGGAGTTTC TCATGCATTC
    AGCAAAGCCT ACCACCATGT GTGTGCACTG GGAAGTGTTC TGCAGATGGC CCTCACTGCT
    CTGGACTGGT TGTATCACAG AGTGCCCAGC ATCCACACTG TCAGTACATA CCTGGTGGAG
    CCTGGCAACG ATCAGACGTG TGACTATGCT CTGCGCAGAA TGGCTACCAC AGACAGTACA
    GGACAAAAGG AAATCCATAC CTCTCCTGTC CTCCTCCTCA GAAAAGAGAA CCTCTTCAGA
    GATTACCTGT TTAAGTGCAC AGACTGCTCA GAGGTCGTTC ACAGTTCTGT CTGTGGAGAG
    CAGCACATTG CAGTGCCTCT CCGGGACCTG TCGGGACGAG CCCTGGGGTT GTTTGATATC
    AGCATCGGAC ACCACCAGGC ATTACCTCCC CACGAACGTA AGGACCTGCA GAGAATGCTG
    AAGATGGCCC AAGCTGCCTG CTCAGAGATA CTGAAGATGC CTTCAGAGGA AACCGAAGCA
    AGCCGTGTCC TGGAAGCAGA ACACAGGAAT GTGAGGCATG CAGGGATTCT CTTCCACCGG
    TTCATGCTGC AGGACCTGCG TGAGCGTATC CAGAAACTGA ATGCTGAGAG CTTTGCTGAA
    ATCAAGAGCT ATGAAGACCC ACCAGCTCTG GTCCATAACA TAATGAAGGC CGTGCTGTTG
    CTTTTGCATC CAGAGTGGGA GGGCACGGAG AAGACTGAGA AGTGGAATCA GTGCATACTG
    CAATTTGACG ACGATTTAAT TCAGGAGATG TATTGCTTTG ATCCAACTGC TGCATCTGTG
    CACGTTCCAG CAGAGATGGT GCAGGACTGT ATCAATGGGG CGCCTCAGGC AGCAGCGTGG
    CCACGGGGCT CCACCACAGC CCGGCTCCTG GAGCTCTGGG CACACACCTG CCTGTGCCTG
    GCCCAGCTGG CAGAGAGCAT GCAGAGCAAC ACGGCCCCGT CCTGCAGCGC TGCCCACAGC
    AACGCCTCCT GTCAGACCCC CGTCTGCTCC CCCAGCCCCA GTGTTCCCCA TATTGGACGC
    CTGTTTCATA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997818.1
    CDS298..3372
    Translation

    Target ORF information:

    RefSeq Version XM_025142050.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGTCTG AGGCAAAAAC CAAGCGTAAG GAAAGAGAAC AGGCAGAAAG GATGAAGTGC 
    CAGCAGAAGG AGATGAGAAA AGCAGCACTG GCAACTCAGT TCAAAGAATG GACAGCGGAT
    GTCAGTGGCA GAATTCCAGT TGCACTGATT CAGAGTGCAC TGAGGATTTT TCTGGATGTC
    TGTGCTTCTA TGCCCGTAGA CACAAGAAAA GCCTATGAGA GGAAGCTGGA AATCACAGAC
    ATGTGGGGGG AAAACGTAAA TGCTGATGGA TTCATAGAGT ATGTCCATTC CTACACGGAG
    AACTTTCCAA ATGATGCATT CCAGGAGGTC CTGAAGCACC TCGGTCAGTG TGCTGCTGAC
    TTCCAAGAAT CCATCAGGCA TAATACGTGC AGGCAGATGT TTATTGACCT CTTCCTACAC
    TGTGATCGAG GACAGGTTGG TGTCTTGGAT AGACCCCAAA TACTTGACCT GCTGGAGCAG
    TTCTATGAGA GGAGCTCTGT GTGTGCCAGG AGGAGATTCT GCAACCCCAA AGAGTGGCCA
    ATAGTCGAGC TGCAGGAGGT CGGTCTGGAG GATCTGTGGG GAGGCCTTGA GGACCAGCAA
    GGTTATGAGG AGCCTGGAGA AGGTTTGTTT TATTCTCAAG CAGAGATTTC TGAGAGAGAC
    GTTTCAGCAC GTGAAGCTGC AGCTGACGAC AGGGCGGGCA GTGATGGAAT GAGCATAATT
    GACAGAGGTC GTTTGGAACA AATGGATGTG AGTGAAACAG AGACTGGAGG AATTCCTACG
    GAAGAAAACC AAGCAGCAGA ATCAGGTGGT GCTGAGGATG CTGTGGCTGC AGAGGCCATG
    AAGGAACCTC CCGCAGGAGA AGAGGGTTTC AGAAGGCAGC ACAGCACCTG GTCTGGGCAG
    CAGACAAGCT CAGGGACAGC AGTGCAGGAC AAGGCCCATC TGCAGGAGCC AGCTGAAGAC
    CTCCATAACA CCATGGCAGG CATTGAGAGC CGCAGTGCTC CTCGTGCTGG GAGCGCCTTC
    GATGGGAGCT GCCTCAACCT GCCGCGCTTT GTTCAGCTCA TGGAGACGTT CCTCGGGGAA
    GGCATGGCTC TGCCTGCCGT GGAGGGGCTC ATTGGGTTCA TCAGAGAGGA ATACAAACAG
    ACCGAAGAGG AAAAAGTGAA ACAACTAGAA AAAGCACATC ACAGCTCCCG TTTGACCCGA
    CGGGAACTGC TTCTGGAAGC ACTGTTTGAA AAGTGGGACA TCAACGCGTC CGGGTTTCTG
    GAGATGAAAG AGGTGGAGGC TGCTCTGAGT GCATTCAAGG AAGGAATGGA AAAAGAGGCC
    GTGAGGATGG CAAAGAACCA CTTTTGCTCA CGTTACCCTC AGCTTGGCAG AGTGGGGAAG
    CTATCCCCAA AGGCATTCCA GGCTTTCCTC GAGTTGGTGG CTTCTGAGCT CACTGGGGAT
    GAAGATGAAG TCATTGATAA CATCGTGGAG TTTCTGACCA CAAGAGTGGA ACGAAGTCAC
    ACGGAGTGCT TGCAAAGCTT GGCGAGGAGG AGATGGCTGC ACAGCATCCA GCAGGCGGCC
    GAGAGGAGCG GAGCACGCAT GGAGCCGGTG TACGACGCGG TGCTCAAGGC CCTCCTCAAG
    GATGCAGAAG CCCACGGGGA TAACAAGAAG ATCAGTGCAT ATATTGCCCT TTTGGAAGAA
    AACCAGCTCT CACCAGAGCG GGGACAGACG CTCTTACGCT ATGTGACATG CACCACAGAT
    GATGCTCCGT ATGTTCTAAA CCAGATACTC TACAGAGACA TGAAAGGAGT AAGCTTTGCA
    GCTGTAGAAG AGGAAAAGCC AATCCATGTG CCAAGAGTTC AGCTGCATGG AAATATCCAC
    TTCTGGAACC AGGACCGCCC AGCTGAGCAG AGGAGAGGCT CATTCCTGGT ACTGCCATTG
    CAGGATGCTC ACTGGAGAGT CTTTGGCACC CTGGGACTTG ATACCCTTCG GGACCCAAGT
    GAGAAAACCA TCTTCCTGAC CCACGAGATC AGCTTCTACC AGGGAGTTTC TCATGCATTC
    AGCAAAGCCT ACCACCATGT GTGTGCACTG GGAAGTGTTC TGCAGATGGC CCTCACTGCT
    CTGGACTGGT TGTATCACAG AGTGCCCAGC ATCCACACTG TCAGTACATA CCTGGTGGAG
    CCTGGCAACG ATCAGACGTG TGACTATGCT CTGCGCAGAA TGGCTACCAC AGACAGTACA
    GGACAAAAGG AAATCCATAC CTCTCCTGTC CTCCTCCTCA GAAAAGAGAA CCTCTTCAGA
    GATTACCTGT TTAAGTGCAC AGACTGCTCA GAGGTCGTTC ACAGTTCTGT CTGTGGAGAG
    CAGCACATTG CAGTGCCTCT CCGGGACCTG TCGGGACGAG CCCTGGGGTT GTTTGATATC
    AGCATCGGAC ACCACCAGGC ATTACCTCCC CACGAACGTA AGGACCTGCA GAGAATGCTG
    AAGATGGCCC AAGCTGCCTG CTCAGAGATA CTGAAGATGC CTTCAGAGGA AACCGAAGCA
    AGCCGTGTCC TGGAAGCAGA ACACAGGAAT GTGAGGCATG CAGGGATTCT CTTCCACCGG
    TTCATGCTGC AGGACCTGCG TGAGCGTATC CAGAAACTGA ATGCTGAGAG CTTTGCTGAA
    ATCAAGAGCT ATGAAGACCC ACCAGCTCTG GTCCATAACA TAATGAAGGC CGTGCTGTTG
    CTTTTGCATC CAGAGTGGGA GGGCACGGAG AAGACTGAGA AGTGGAATCA GTGCATACTG
    CAATTTGACG ACGATTTAAT TCAGGAGATG TATTGCTTTG ATCCAACTGC TGCATCTGTG
    CACGTTCCAG CAGAGATGGT GCAGGACTGT ATCAATGGGG CGCCTCAGGC AGCAGCGTGG
    CCACGGGGCT CCACCACAGC CCGGCTCCTG GAGCTCTGGG CACACACCTG CCTGTGCCTG
    GCCCAGCTGG CAGAGAGCAT GCAGAGCAAC ACGGCCCCGT CCTGCAGCGC TGCCCACAGC
    AACGCCTCCT GTCAGACCCC CGTCTGCTCC CCCAGCCCCA GTGTTCCCCA TATTGGACGC
    CTGTTTCATA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa145640
    Clone ID Related Accession (Same CDS sequence) XM_025142050.1 , XM_025142051.1
    Accession Version XM_025142051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGTCTG AGGCAAAAAC CAAGCGTAAG GAAAGAGAAC AGGCAGAAAG GATGAAGTGC 
    CAGCAGAAGG AGATGAGAAA AGCAGCACTG GCAACTCAGT TCAAAGAATG GACAGCGGAT
    GTCAGTGGCA GAATTCCAGT TGCACTGATT CAGAGTGCAC TGAGGATTTT TCTGGATGTC
    TGTGCTTCTA TGCCCGTAGA CACAAGAAAA GCCTATGAGA GGAAGCTGGA AATCACAGAC
    ATGTGGGGGG AAAACGTAAA TGCTGATGGA TTCATAGAGT ATGTCCATTC CTACACGGAG
    AACTTTCCAA ATGATGCATT CCAGGAGGTC CTGAAGCACC TCGGTCAGTG TGCTGCTGAC
    TTCCAAGAAT CCATCAGGCA TAATACGTGC AGGCAGATGT TTATTGACCT CTTCCTACAC
    TGTGATCGAG GACAGGTTGG TGTCTTGGAT AGACCCCAAA TACTTGACCT GCTGGAGCAG
    TTCTATGAGA GGAGCTCTGT GTGTGCCAGG AGGAGATTCT GCAACCCCAA AGAGTGGCCA
    ATAGTCGAGC TGCAGGAGGT CGGTCTGGAG GATCTGTGGG GAGGCCTTGA GGACCAGCAA
    GGTTATGAGG AGCCTGGAGA AGGTTTGTTT TATTCTCAAG CAGAGATTTC TGAGAGAGAC
    GTTTCAGCAC GTGAAGCTGC AGCTGACGAC AGGGCGGGCA GTGATGGAAT GAGCATAATT
    GACAGAGGTC GTTTGGAACA AATGGATGTG AGTGAAACAG AGACTGGAGG AATTCCTACG
    GAAGAAAACC AAGCAGCAGA ATCAGGTGGT GCTGAGGATG CTGTGGCTGC AGAGGCCATG
    AAGGAACCTC CCGCAGGAGA AGAGGGTTTC AGAAGGCAGC ACAGCACCTG GTCTGGGCAG
    CAGACAAGCT CAGGGACAGC AGTGCAGGAC AAGGCCCATC TGCAGGAGCC AGCTGAAGAC
    CTCCATAACA CCATGGCAGG CATTGAGAGC CGCAGTGCTC CTCGTGCTGG GAGCGCCTTC
    GATGGGAGCT GCCTCAACCT GCCGCGCTTT GTTCAGCTCA TGGAGACGTT CCTCGGGGAA
    GGCATGGCTC TGCCTGCCGT GGAGGGGCTC ATTGGGTTCA TCAGAGAGGA ATACAAACAG
    ACCGAAGAGG AAAAAGTGAA ACAACTAGAA AAAGCACATC ACAGCTCCCG TTTGACCCGA
    CGGGAACTGC TTCTGGAAGC ACTGTTTGAA AAGTGGGACA TCAACGCGTC CGGGTTTCTG
    GAGATGAAAG AGGTGGAGGC TGCTCTGAGT GCATTCAAGG AAGGAATGGA AAAAGAGGCC
    GTGAGGATGG CAAAGAACCA CTTTTGCTCA CGTTACCCTC AGCTTGGCAG AGTGGGGAAG
    CTATCCCCAA AGGCATTCCA GGCTTTCCTC GAGTTGGTGG CTTCTGAGCT CACTGGGGAT
    GAAGATGAAG TCATTGATAA CATCGTGGAG TTTCTGACCA CAAGAGTGGA ACGAAGTCAC
    ACGGAGTGCT TGCAAAGCTT GGCGAGGAGG AGATGGCTGC ACAGCATCCA GCAGGCGGCC
    GAGAGGAGCG GAGCACGCAT GGAGCCGGTG TACGACGCGG TGCTCAAGGC CCTCCTCAAG
    GATGCAGAAG CCCACGGGGA TAACAAGAAG ATCAGTGCAT ATATTGCCCT TTTGGAAGAA
    AACCAGCTCT CACCAGAGCG GGGACAGACG CTCTTACGCT ATGTGACATG CACCACAGAT
    GATGCTCCGT ATGTTCTAAA CCAGATACTC TACAGAGACA TGAAAGGAGT AAGCTTTGCA
    GCTGTAGAAG AGGAAAAGCC AATCCATGTG CCAAGAGTTC AGCTGCATGG AAATATCCAC
    TTCTGGAACC AGGACCGCCC AGCTGAGCAG AGGAGAGGCT CATTCCTGGT ACTGCCATTG
    CAGGATGCTC ACTGGAGAGT CTTTGGCACC CTGGGACTTG ATACCCTTCG GGACCCAAGT
    GAGAAAACCA TCTTCCTGAC CCACGAGATC AGCTTCTACC AGGGAGTTTC TCATGCATTC
    AGCAAAGCCT ACCACCATGT GTGTGCACTG GGAAGTGTTC TGCAGATGGC CCTCACTGCT
    CTGGACTGGT TGTATCACAG AGTGCCCAGC ATCCACACTG TCAGTACATA CCTGGTGGAG
    CCTGGCAACG ATCAGACGTG TGACTATGCT CTGCGCAGAA TGGCTACCAC AGACAGTACA
    GGACAAAAGG AAATCCATAC CTCTCCTGTC CTCCTCCTCA GAAAAGAGAA CCTCTTCAGA
    GATTACCTGT TTAAGTGCAC AGACTGCTCA GAGGTCGTTC ACAGTTCTGT CTGTGGAGAG
    CAGCACATTG CAGTGCCTCT CCGGGACCTG TCGGGACGAG CCCTGGGGTT GTTTGATATC
    AGCATCGGAC ACCACCAGGC ATTACCTCCC CACGAACGTA AGGACCTGCA GAGAATGCTG
    AAGATGGCCC AAGCTGCCTG CTCAGAGATA CTGAAGATGC CTTCAGAGGA AACCGAAGCA
    AGCCGTGTCC TGGAAGCAGA ACACAGGAAT GTGAGGCATG CAGGGATTCT CTTCCACCGG
    TTCATGCTGC AGGACCTGCG TGAGCGTATC CAGAAACTGA ATGCTGAGAG CTTTGCTGAA
    ATCAAGAGCT ATGAAGACCC ACCAGCTCTG GTCCATAACA TAATGAAGGC CGTGCTGTTG
    CTTTTGCATC CAGAGTGGGA GGGCACGGAG AAGACTGAGA AGTGGAATCA GTGCATACTG
    CAATTTGACG ACGATTTAAT TCAGGAGATG TATTGCTTTG ATCCAACTGC TGCATCTGTG
    CACGTTCCAG CAGAGATGGT GCAGGACTGT ATCAATGGGG CGCCTCAGGC AGCAGCGTGG
    CCACGGGGCT CCACCACAGC CCGGCTCCTG GAGCTCTGGG CACACACCTG CCTGTGCCTG
    GCCCAGCTGG CAGAGAGCAT GCAGAGCAAC ACGGCCCCGT CCTGCAGCGC TGCCCACAGC
    AACGCCTCCT GTCAGACCCC CGTCTGCTCC CCCAGCCCCA GTGTTCCCCA TATTGGACGC
    CTGTTTCATA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997819.1
    CDS3669..6743
    Translation

    Target ORF information:

    RefSeq Version XM_025142051.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGTCTG AGGCAAAAAC CAAGCGTAAG GAAAGAGAAC AGGCAGAAAG GATGAAGTGC 
    CAGCAGAAGG AGATGAGAAA AGCAGCACTG GCAACTCAGT TCAAAGAATG GACAGCGGAT
    GTCAGTGGCA GAATTCCAGT TGCACTGATT CAGAGTGCAC TGAGGATTTT TCTGGATGTC
    TGTGCTTCTA TGCCCGTAGA CACAAGAAAA GCCTATGAGA GGAAGCTGGA AATCACAGAC
    ATGTGGGGGG AAAACGTAAA TGCTGATGGA TTCATAGAGT ATGTCCATTC CTACACGGAG
    AACTTTCCAA ATGATGCATT CCAGGAGGTC CTGAAGCACC TCGGTCAGTG TGCTGCTGAC
    TTCCAAGAAT CCATCAGGCA TAATACGTGC AGGCAGATGT TTATTGACCT CTTCCTACAC
    TGTGATCGAG GACAGGTTGG TGTCTTGGAT AGACCCCAAA TACTTGACCT GCTGGAGCAG
    TTCTATGAGA GGAGCTCTGT GTGTGCCAGG AGGAGATTCT GCAACCCCAA AGAGTGGCCA
    ATAGTCGAGC TGCAGGAGGT CGGTCTGGAG GATCTGTGGG GAGGCCTTGA GGACCAGCAA
    GGTTATGAGG AGCCTGGAGA AGGTTTGTTT TATTCTCAAG CAGAGATTTC TGAGAGAGAC
    GTTTCAGCAC GTGAAGCTGC AGCTGACGAC AGGGCGGGCA GTGATGGAAT GAGCATAATT
    GACAGAGGTC GTTTGGAACA AATGGATGTG AGTGAAACAG AGACTGGAGG AATTCCTACG
    GAAGAAAACC AAGCAGCAGA ATCAGGTGGT GCTGAGGATG CTGTGGCTGC AGAGGCCATG
    AAGGAACCTC CCGCAGGAGA AGAGGGTTTC AGAAGGCAGC ACAGCACCTG GTCTGGGCAG
    CAGACAAGCT CAGGGACAGC AGTGCAGGAC AAGGCCCATC TGCAGGAGCC AGCTGAAGAC
    CTCCATAACA CCATGGCAGG CATTGAGAGC CGCAGTGCTC CTCGTGCTGG GAGCGCCTTC
    GATGGGAGCT GCCTCAACCT GCCGCGCTTT GTTCAGCTCA TGGAGACGTT CCTCGGGGAA
    GGCATGGCTC TGCCTGCCGT GGAGGGGCTC ATTGGGTTCA TCAGAGAGGA ATACAAACAG
    ACCGAAGAGG AAAAAGTGAA ACAACTAGAA AAAGCACATC ACAGCTCCCG TTTGACCCGA
    CGGGAACTGC TTCTGGAAGC ACTGTTTGAA AAGTGGGACA TCAACGCGTC CGGGTTTCTG
    GAGATGAAAG AGGTGGAGGC TGCTCTGAGT GCATTCAAGG AAGGAATGGA AAAAGAGGCC
    GTGAGGATGG CAAAGAACCA CTTTTGCTCA CGTTACCCTC AGCTTGGCAG AGTGGGGAAG
    CTATCCCCAA AGGCATTCCA GGCTTTCCTC GAGTTGGTGG CTTCTGAGCT CACTGGGGAT
    GAAGATGAAG TCATTGATAA CATCGTGGAG TTTCTGACCA CAAGAGTGGA ACGAAGTCAC
    ACGGAGTGCT TGCAAAGCTT GGCGAGGAGG AGATGGCTGC ACAGCATCCA GCAGGCGGCC
    GAGAGGAGCG GAGCACGCAT GGAGCCGGTG TACGACGCGG TGCTCAAGGC CCTCCTCAAG
    GATGCAGAAG CCCACGGGGA TAACAAGAAG ATCAGTGCAT ATATTGCCCT TTTGGAAGAA
    AACCAGCTCT CACCAGAGCG GGGACAGACG CTCTTACGCT ATGTGACATG CACCACAGAT
    GATGCTCCGT ATGTTCTAAA CCAGATACTC TACAGAGACA TGAAAGGAGT AAGCTTTGCA
    GCTGTAGAAG AGGAAAAGCC AATCCATGTG CCAAGAGTTC AGCTGCATGG AAATATCCAC
    TTCTGGAACC AGGACCGCCC AGCTGAGCAG AGGAGAGGCT CATTCCTGGT ACTGCCATTG
    CAGGATGCTC ACTGGAGAGT CTTTGGCACC CTGGGACTTG ATACCCTTCG GGACCCAAGT
    GAGAAAACCA TCTTCCTGAC CCACGAGATC AGCTTCTACC AGGGAGTTTC TCATGCATTC
    AGCAAAGCCT ACCACCATGT GTGTGCACTG GGAAGTGTTC TGCAGATGGC CCTCACTGCT
    CTGGACTGGT TGTATCACAG AGTGCCCAGC ATCCACACTG TCAGTACATA CCTGGTGGAG
    CCTGGCAACG ATCAGACGTG TGACTATGCT CTGCGCAGAA TGGCTACCAC AGACAGTACA
    GGACAAAAGG AAATCCATAC CTCTCCTGTC CTCCTCCTCA GAAAAGAGAA CCTCTTCAGA
    GATTACCTGT TTAAGTGCAC AGACTGCTCA GAGGTCGTTC ACAGTTCTGT CTGTGGAGAG
    CAGCACATTG CAGTGCCTCT CCGGGACCTG TCGGGACGAG CCCTGGGGTT GTTTGATATC
    AGCATCGGAC ACCACCAGGC ATTACCTCCC CACGAACGTA AGGACCTGCA GAGAATGCTG
    AAGATGGCCC AAGCTGCCTG CTCAGAGATA CTGAAGATGC CTTCAGAGGA AACCGAAGCA
    AGCCGTGTCC TGGAAGCAGA ACACAGGAAT GTGAGGCATG CAGGGATTCT CTTCCACCGG
    TTCATGCTGC AGGACCTGCG TGAGCGTATC CAGAAACTGA ATGCTGAGAG CTTTGCTGAA
    ATCAAGAGCT ATGAAGACCC ACCAGCTCTG GTCCATAACA TAATGAAGGC CGTGCTGTTG
    CTTTTGCATC CAGAGTGGGA GGGCACGGAG AAGACTGAGA AGTGGAATCA GTGCATACTG
    CAATTTGACG ACGATTTAAT TCAGGAGATG TATTGCTTTG ATCCAACTGC TGCATCTGTG
    CACGTTCCAG CAGAGATGGT GCAGGACTGT ATCAATGGGG CGCCTCAGGC AGCAGCGTGG
    CCACGGGGCT CCACCACAGC CCGGCTCCTG GAGCTCTGGG CACACACCTG CCTGTGCCTG
    GCCCAGCTGG CAGAGAGCAT GCAGAGCAAC ACGGCCCCGT CCTGCAGCGC TGCCCACAGC
    AACGCCTCCT GTCAGACCCC CGTCTGCTCC CCCAGCCCCA GTGTTCCCCA TATTGGACGC
    CTGTTTCATA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17247
    Clone ID Related Accession (Same CDS sequence) XM_015296106.1 , XM_025142048.1 , XM_025142049.1 , XM_015296106.2 , XM_015296104.1 , XM_015296105.1
    Accession Version XM_015296106.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151592.1
    CDS579..4232
    Translation

    Target ORF information:

    RefSeq Version XM_015296106.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296106.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17247
    Clone ID Related Accession (Same CDS sequence) XM_015296106.1 , XM_025142048.1 , XM_025142049.1 , XM_015296106.2 , XM_015296104.1 , XM_015296105.1
    Accession Version XM_025142048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997816.1
    CDS3140..6793
    Translation

    Target ORF information:

    RefSeq Version XM_025142048.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17247
    Clone ID Related Accession (Same CDS sequence) XM_015296106.1 , XM_025142048.1 , XM_025142049.1 , XM_015296106.2 , XM_015296104.1 , XM_015296105.1
    Accession Version XM_025142049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997817.1
    CDS3014..6667
    Translation

    Target ORF information:

    RefSeq Version XM_025142049.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17247
    Clone ID Related Accession (Same CDS sequence) XM_015296106.1 , XM_025142048.1 , XM_025142049.1 , XM_015296106.2 , XM_015296104.1 , XM_015296105.1
    Accession Version XM_015296106.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151592.1
    CDS577..4230
    Translation

    Target ORF information:

    RefSeq Version XM_015296106.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17247
    Clone ID Related Accession (Same CDS sequence) XM_015296106.1 , XM_025142048.1 , XM_025142049.1 , XM_015296106.2 , XM_015296104.1 , XM_015296105.1
    Accession Version XM_015296104.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151590.1
    CDS574..4227
    Translation

    Target ORF information:

    RefSeq Version XM_015296104.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296104.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17247
    Clone ID Related Accession (Same CDS sequence) XM_015296106.1 , XM_025142048.1 , XM_025142049.1 , XM_015296106.2 , XM_015296104.1 , XM_015296105.1
    Accession Version XM_015296105.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151591.1
    CDS635..4288
    Translation

    Target ORF information:

    RefSeq Version XM_015296105.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296105.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGAAGCAGAA
    CACAGGAATG TGAGGCATGC AGGGATTCTC TTCCACCGGT TCATGCTGCA GGACCTGCGT
    GAGCGTATCC AGAAACTGAA TGCTGAGAGC TTTGCTGAAA TCAAGAGCTA TGAAGACCCA
    CCAGCTCTGG TCCATAACAT AATGAAGGCC GTGCTGTTGC TTTTGCATCC AGAGTGGGAG
    GGCACGGAGA AGACTGAGAA GTGGAATCAG TGCATACTGC AATTTGACGA CGATTTAATT
    CAGGAGATGT ATTGCTTTGA TCCAACTGCT GCATCTGTGC ACGTTCCAGC AGAGATGGTG
    CAGGACTGTA TCAATGGGGC GCCTCAGGCA GCAGCGTGGC CACGGGGCTC CACCACAGCC
    CGGCTCCTGG AGCTCTGGGC ACACACCTGC CTGTGCCTGG CCCAGCTGGC AGAGAGCATG
    CAGAGCAACA CGGCCCCGTC CTGCAGCGCT GCCCACAGCA ACGCCTCCTG TCAGACCCCC
    GTCTGCTCCC CCAGCCCCAG TGTTCCCCAT ATTGGACGCC TGTTTCATAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17248
    Clone ID Related Accession (Same CDS sequence) XM_004946638.2 , XM_004946638.3
    Accession Version XM_004946638.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GCTCATACAG AACTGCCCTG GCTCATTTGG AGTGTTCTGC
    TTTCACAGGG AGTTTCTCAT GCATTCAGCA AAGCCTACCA CCATGTGTGT GCACTGGGAA
    GTGTTCTGCA GATGGCCCTC ACTGCTCTGG ACTGGTTGTA TCACAGAGTG CCCAGCATCC
    ACACTGTCAG TACATACCTG GTGGAGCCTG GCAACGATCA GAGATTACCT GTTTAAGTGC
    ACAGACTGCT CAGAGGTCGT TCACAGTTCT GTCTGTGGAG AGCAGCACAT TGCAGTGCCT
    CTCCGGGACC TGTCGGGACG AGCCCTGGGG TTGTTTGATA TCAGCATCGG ACACCACCAG
    GCATTACCTC CCCACGAACG TAAGGACCTG CAGAGAATGC TGAAGATGGC CCAAGCTGCC
    TGCTCAGAGA TACTGAAGAT GCCTTCAGAG GAAACCGAAG CAAGCCGTGT CCTGGAAGCA
    GAACACAGGA ATGTGAGGCA TGCAGGGATT CTCTTCCACC GGTTCATGCT GCAGGACCTG
    CGTGAGCGTA TCCAGAAACT GAATGCTGAG AGCTTTGCTG AAATCAAGAG CTATGAAGAC
    CCACCAGCTC TGGTCCATAA CATAATGAAG GCCGTGCTGT TGCTTTTGCA TCCAGAGTGG
    GAGGGCACGG AGAAGACTGA GAAGTGGAAT CAGTGCATAC TGCAATTTGA CGACGATTTA
    ATTCAGGAGA TGTATTGCTT TGATCCAACT GCTGCATCTG TGCACGTTCC AGCAGAGATG
    GTGCAGGACT GTATCAATGG GGCGCCTCAG GCAGCAGCGT GGCCACGGGG CTCCACCACA
    GCCCGGCTCC TGGAGCTCTG GGCACACACC TGCCTGTGCC TGGCCCAGCT GGCAGAGAGC
    ATGCAGAGCA ACACGGCCCC GTCCTGCAGC GCTGCCCACA GCAACGCCTC CTGTCAGACC
    CCCGTCTGCT CCCCCAGCCC CAGTGTTCCC CATATTGGAC GCCTGTTTCA TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004946695.1
    CDS1639..5235
    Translation

    Target ORF information:

    RefSeq Version XM_004946638.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004946638.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GCTCATACAG AACTGCCCTG GCTCATTTGG AGTGTTCTGC
    TTTCACAGGG AGTTTCTCAT GCATTCAGCA AAGCCTACCA CCATGTGTGT GCACTGGGAA
    GTGTTCTGCA GATGGCCCTC ACTGCTCTGG ACTGGTTGTA TCACAGAGTG CCCAGCATCC
    ACACTGTCAG TACATACCTG GTGGAGCCTG GCAACGATCA GAGATTACCT GTTTAAGTGC
    ACAGACTGCT CAGAGGTCGT TCACAGTTCT GTCTGTGGAG AGCAGCACAT TGCAGTGCCT
    CTCCGGGACC TGTCGGGACG AGCCCTGGGG TTGTTTGATA TCAGCATCGG ACACCACCAG
    GCATTACCTC CCCACGAACG TAAGGACCTG CAGAGAATGC TGAAGATGGC CCAAGCTGCC
    TGCTCAGAGA TACTGAAGAT GCCTTCAGAG GAAACCGAAG CAAGCCGTGT CCTGGAAGCA
    GAACACAGGA ATGTGAGGCA TGCAGGGATT CTCTTCCACC GGTTCATGCT GCAGGACCTG
    CGTGAGCGTA TCCAGAAACT GAATGCTGAG AGCTTTGCTG AAATCAAGAG CTATGAAGAC
    CCACCAGCTC TGGTCCATAA CATAATGAAG GCCGTGCTGT TGCTTTTGCA TCCAGAGTGG
    GAGGGCACGG AGAAGACTGA GAAGTGGAAT CAGTGCATAC TGCAATTTGA CGACGATTTA
    ATTCAGGAGA TGTATTGCTT TGATCCAACT GCTGCATCTG TGCACGTTCC AGCAGAGATG
    GTGCAGGACT GTATCAATGG GGCGCCTCAG GCAGCAGCGT GGCCACGGGG CTCCACCACA
    GCCCGGCTCC TGGAGCTCTG GGCACACACC TGCCTGTGCC TGGCCCAGCT GGCAGAGAGC
    ATGCAGAGCA ACACGGCCCC GTCCTGCAGC GCTGCCCACA GCAACGCCTC CTGTCAGACC
    CCCGTCTGCT CCCCCAGCCC CAGTGTTCCC CATATTGGAC GCCTGTTTCA TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17248
    Clone ID Related Accession (Same CDS sequence) XM_004946638.2 , XM_004946638.3
    Accession Version XM_004946638.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004946638.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GCTCATACAG AACTGCCCTG GCTCATTTGG AGTGTTCTGC
    TTTCACAGGG AGTTTCTCAT GCATTCAGCA AAGCCTACCA CCATGTGTGT GCACTGGGAA
    GTGTTCTGCA GATGGCCCTC ACTGCTCTGG ACTGGTTGTA TCACAGAGTG CCCAGCATCC
    ACACTGTCAG TACATACCTG GTGGAGCCTG GCAACGATCA GAGATTACCT GTTTAAGTGC
    ACAGACTGCT CAGAGGTCGT TCACAGTTCT GTCTGTGGAG AGCAGCACAT TGCAGTGCCT
    CTCCGGGACC TGTCGGGACG AGCCCTGGGG TTGTTTGATA TCAGCATCGG ACACCACCAG
    GCATTACCTC CCCACGAACG TAAGGACCTG CAGAGAATGC TGAAGATGGC CCAAGCTGCC
    TGCTCAGAGA TACTGAAGAT GCCTTCAGAG GAAACCGAAG CAAGCCGTGT CCTGGAAGCA
    GAACACAGGA ATGTGAGGCA TGCAGGGATT CTCTTCCACC GGTTCATGCT GCAGGACCTG
    CGTGAGCGTA TCCAGAAACT GAATGCTGAG AGCTTTGCTG AAATCAAGAG CTATGAAGAC
    CCACCAGCTC TGGTCCATAA CATAATGAAG GCCGTGCTGT TGCTTTTGCA TCCAGAGTGG
    GAGGGCACGG AGAAGACTGA GAAGTGGAAT CAGTGCATAC TGCAATTTGA CGACGATTTA
    ATTCAGGAGA TGTATTGCTT TGATCCAACT GCTGCATCTG TGCACGTTCC AGCAGAGATG
    GTGCAGGACT GTATCAATGG GGCGCCTCAG GCAGCAGCGT GGCCACGGGG CTCCACCACA
    GCCCGGCTCC TGGAGCTCTG GGCACACACC TGCCTGTGCC TGGCCCAGCT GGCAGAGAGC
    ATGCAGAGCA ACACGGCCCC GTCCTGCAGC GCTGCCCACA GCAACGCCTC CTGTCAGACC
    CCCGTCTGCT CCCCCAGCCC CAGTGTTCCC CATATTGGAC GCCTGTTTCA TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004946695.1
    CDS3140..6736
    Translation

    Target ORF information:

    RefSeq Version XM_004946638.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004946638.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GCTCATACAG AACTGCCCTG GCTCATTTGG AGTGTTCTGC
    TTTCACAGGG AGTTTCTCAT GCATTCAGCA AAGCCTACCA CCATGTGTGT GCACTGGGAA
    GTGTTCTGCA GATGGCCCTC ACTGCTCTGG ACTGGTTGTA TCACAGAGTG CCCAGCATCC
    ACACTGTCAG TACATACCTG GTGGAGCCTG GCAACGATCA GAGATTACCT GTTTAAGTGC
    ACAGACTGCT CAGAGGTCGT TCACAGTTCT GTCTGTGGAG AGCAGCACAT TGCAGTGCCT
    CTCCGGGACC TGTCGGGACG AGCCCTGGGG TTGTTTGATA TCAGCATCGG ACACCACCAG
    GCATTACCTC CCCACGAACG TAAGGACCTG CAGAGAATGC TGAAGATGGC CCAAGCTGCC
    TGCTCAGAGA TACTGAAGAT GCCTTCAGAG GAAACCGAAG CAAGCCGTGT CCTGGAAGCA
    GAACACAGGA ATGTGAGGCA TGCAGGGATT CTCTTCCACC GGTTCATGCT GCAGGACCTG
    CGTGAGCGTA TCCAGAAACT GAATGCTGAG AGCTTTGCTG AAATCAAGAG CTATGAAGAC
    CCACCAGCTC TGGTCCATAA CATAATGAAG GCCGTGCTGT TGCTTTTGCA TCCAGAGTGG
    GAGGGCACGG AGAAGACTGA GAAGTGGAAT CAGTGCATAC TGCAATTTGA CGACGATTTA
    ATTCAGGAGA TGTATTGCTT TGATCCAACT GCTGCATCTG TGCACGTTCC AGCAGAGATG
    GTGCAGGACT GTATCAATGG GGCGCCTCAG GCAGCAGCGT GGCCACGGGG CTCCACCACA
    GCCCGGCTCC TGGAGCTCTG GGCACACACC TGCCTGTGCC TGGCCCAGCT GGCAGAGAGC
    ATGCAGAGCA ACACGGCCCC GTCCTGCAGC GCTGCCCACA GCAACGCCTC CTGTCAGACC
    CCCGTCTGCT CCCCCAGCCC CAGTGTTCCC CATATTGGAC GCCTGTTTCA TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17249
    Clone ID Related Accession (Same CDS sequence) XM_015296107.1 , XM_015296107.2
    Accession Version XM_015296107.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAAGTAG AAAGGAAGGA CGTGCTTGCA GCAGCCGAGG AGCACGCCAG GCTGGACCCC 
    ATTACTTTTC TTGGAGAATA TCTGATGAGG CATAACCCGC AGTATGACAC CTCTACCAAC
    CCAGGGCCCT ACCTGAGGGG ACTGAAGGCG GCCACAGAGG AGCTAAGAAG GGAGATCCCA
    GATACGGCAT CGGAGAGGCT GGCCAGGATG AAGTCTGAGG CAAAAACCAA GCGTAAGGAA
    AGAGAACAGG CAGAAAGGAT GAAGTGCCAG CAGAAGGAGA TGAGAAAAGC AGCACTGGCA
    ACTCAGTTCA AAGAATGGAC AGCGGATGTC AGTGGCAGAA TTCCAGTTGC ACTGATTCAG
    AGTGCACTGA GGATTTTTCT GGATGTCTGT GCTTCTATGC CCGTAGACAC AAGAAAAGCC
    TATGAGAGGA AGCTGGAAAT CACAGACATG TGGGGGGAAA ACGTAAATGC TGATGGATTC
    ATAGAGTATG TCCATTCCTA CACGGAGAAC TTTCCAAATG ATGCATTCCA GGAGGTCCTG
    AAGCACCTCG GTCAGTGTGC TGCTGACTTC CAAGAATCCA TCAGGCATAA TACGTGCAGG
    CAGATGTTTA TTGACCTCTT CCTACACTGT GATCGAGGAC AGGTTGGTGT CTTGGATAGA
    CCCCAAATAC TTGACCTGCT GGAGCAGTTC TATGAGAGGA GCTCTGTGTG TGCCAGGAGG
    AGATTCTGCA ACCCCAAAGA GTGGCCAATA GTCGAGCTGC AGGAGGTCGG TCTGGAGGAT
    CTGTGGGGAG GCCTTGAGGA CCAGCAAGGT TATGAGGAGC CTGGAGAAGG TTTGTTTTAT
    TCTCAAGCAG AGATTTCTGA GAGAGACGTT TCAGCACGTG AAGCTGCAGC TGACGACAGG
    GCGGGCAGTG ATGGAATGAG CATAATTGAC AGAGGTCGTT TGGAACAAAT GGATGTGAGT
    GAAACAGAGA CTGGAGGAAT TCCTACGGAA GAAAACCAAG CAGCAGAATC AGGTGGTGCT
    GAGGATGCTG TGGCTGCAGA GGCCATGAAG GAACCTCCCG CAGGAGAAGA GGGTTTCAGA
    AGGCAGCACA GCACCTGGTC TGGGCAGCAG ACAAGCTCAG GGACAGCAGT GCAGGACAAG
    GCCCATCTGC AGGAGCCAGC TGAAGACCTC CATAACACCA TGGCAGGCAT TGAGAGCCGC
    AGTGCTCCTC GTGCTGGGAG CGCCTTCGAT GGGAGCTGCC TCAACCTGCC GCGCTTTGTT
    CAGCTCATGG AGACGTTCCT CGGGGAAGGC ATGGCTCTGC CTGCCGTGGA GGGGCTCATT
    GGGTTCATCA GAGAGGAATA CAAACAGACC GAAGAGGAAA AAGTGAAACA ACTAGAAAAA
    GCACATCACA GCTCCCGTTT GACCCGACGG GAACTGCTTC TGGAAGCACT GTTTGAAAAG
    TGGGACATCA ACGCGTCCGG GTTTCTGGAG ATGAAAGAGG TGGAGGCTGC TCTGAGTGCA
    TTCAAGGAAG GAATGGAAAA AGAGGCCGTG AGGATGGCAA AGAACCACTT TTGCTCACGT
    TACCCTCAGC TTGGCAGAGT GGGGAAGCTA TCCCCAAAGG CATTCCAGGC TTTCCTCGAG
    TTGGTGGCTT CTGAGCTCAC TGGGGATGAA GATGAAGTCA TTGATAACAT CGTGGAGTTT
    CTGACCACAA GAGTGGAACG AAGTCACACG GAGTGCTTGC AAAGCTTGGC GAGGAGGAGA
    TGGCTGCACA GCATCCAGCA GGCGGCCGAG AGGAGCGGAG CACGCATGGA GCCGGTGTAC
    GACGCGGTGC TCAAGGCCCT CCTCAAGGAT GCAGAAGCCC ACGGGGATAA CAAGAAGATC
    AGTGCATATA TTGCCCTTTT GGAAGAAAAC CAGCTCTCAC CAGAGCGGGG ACAGACGCTC
    TTACGCTATG TGACATGCAC CACAGATGAT GCTCCGTATG TTCTAAACCA GATACTCTAC
    AGAGACATGA AAGGAGTAAG CTTTGCAGCT GTAGAAGAGG AAAAGCCAAT CCATGTGCCA
    AGAGTTCAGC TGCATGGAAA TATCCACTTC TGGAACCAGG ACCGCCCAGC TGAGCAGAGG
    AGAGGCTCAT TCCTGGTACT GCCATTGCAG GATGCTCACT GGAGAGTCTT TGGCACCCTG
    GGACTTGATA CCCTTCGGGA CCCAAGTGAG AAAACCATCT TCCTGACCCA CGAGATCAGC
    TTCTACCAGG GAGTTTCTCA TGCATTCAGC AAAGCCTACC ACCATGTGTG TGCACTGGGA
    AGTGTTCTGC AGATGGCCCT CACTGCTCTG GACTGGTTGT ATCACAGAGT GCCCAGCATC
    CACACTGTCA GTACATACCT GGTGGAGCCT GGCAACGATC AGACGTGTGA CTATGCTCTG
    CGCAGAATGG CTACCACAGA CAGTACAGGA CAAAAGGAAA TCCATACCTC TCCTGTCCTC
    CTCCTCAGAA AAGAGAACCT CTTCAGAGAT TACCTGTTTA AGTGCACAGA CTGCTCAGAG
    GTCGTTCACA GTTCTGTCTG TGGAGAGCAG CACATTGCAG TGCCTCTCCG GGACCTGTCG
    GGACGAGCCC TGGGGTTGTT TGATATCAGC ATCGGACACC ACCAGGCATT ACCTCCCCAC
    GAACGTAAGG ACCTGCAGAG AATGCTGAAG ATGGCCCAAG CTGCCTGCTC AGAGATACTG
    AAGATGCCTT CAGAGGAAAC CGAAGCAAGC CGTGTCCTGG AAGCAGAACA CAGGAATGTG
    AGGCATGCAG GGATTCTCTT CCACCGGTTC ATGCTGCAGG ACCTGCGTGA GCGTATCCAG
    AAACTGAATG CTGAGAGCTT TGCTGAAATC AAGAGCTATG AAGACCCACC AGCTCTGGTC
    CATAACATAA TGAAGGCCGT GCTGTTGCTT TTGCATCCAG AGTGGGAGGG CACGGAGAAG
    ACTGAGAAGT GGAATCAGTG CATACTGCAA TTTGACGACG ATTTAATTCA GGAGATGTAT
    TGCTTTGATC CAACTGCTGC ATCTGTGCAC GTTCCAGCAG AGATGGTGCA GGACTGTATC
    AATGGGGCGC CTCAGGCAGC AGCGTGGCCA CGGGGCTCCA CCACAGCCCG GCTCCTGGAG
    CTCTGGGCAC ACACCTGCCT GTGCCTGGCC CAGCTGGCAG AGAGCATGCA GAGCAACACG
    GCCCCGTCCT GCAGCGCTGC CCACAGCAAC GCCTCCTGTC AGACCCCCGT CTGCTCCCCC
    AGCCCCAGTG TTCCCCATAT TGGACGCCTG TTTCATAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151593.1
    CDS1896..5177
    Translation

    Target ORF information:

    RefSeq Version XM_015296107.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAAGTAG AAAGGAAGGA CGTGCTTGCA GCAGCCGAGG AGCACGCCAG GCTGGACCCC 
    ATTACTTTTC TTGGAGAATA TCTGATGAGG CATAACCCGC AGTATGACAC CTCTACCAAC
    CCAGGGCCCT ACCTGAGGGG ACTGAAGGCG GCCACAGAGG AGCTAAGAAG GGAGATCCCA
    GATACGGCAT CGGAGAGGCT GGCCAGGATG AAGTCTGAGG CAAAAACCAA GCGTAAGGAA
    AGAGAACAGG CAGAAAGGAT GAAGTGCCAG CAGAAGGAGA TGAGAAAAGC AGCACTGGCA
    ACTCAGTTCA AAGAATGGAC AGCGGATGTC AGTGGCAGAA TTCCAGTTGC ACTGATTCAG
    AGTGCACTGA GGATTTTTCT GGATGTCTGT GCTTCTATGC CCGTAGACAC AAGAAAAGCC
    TATGAGAGGA AGCTGGAAAT CACAGACATG TGGGGGGAAA ACGTAAATGC TGATGGATTC
    ATAGAGTATG TCCATTCCTA CACGGAGAAC TTTCCAAATG ATGCATTCCA GGAGGTCCTG
    AAGCACCTCG GTCAGTGTGC TGCTGACTTC CAAGAATCCA TCAGGCATAA TACGTGCAGG
    CAGATGTTTA TTGACCTCTT CCTACACTGT GATCGAGGAC AGGTTGGTGT CTTGGATAGA
    CCCCAAATAC TTGACCTGCT GGAGCAGTTC TATGAGAGGA GCTCTGTGTG TGCCAGGAGG
    AGATTCTGCA ACCCCAAAGA GTGGCCAATA GTCGAGCTGC AGGAGGTCGG TCTGGAGGAT
    CTGTGGGGAG GCCTTGAGGA CCAGCAAGGT TATGAGGAGC CTGGAGAAGG TTTGTTTTAT
    TCTCAAGCAG AGATTTCTGA GAGAGACGTT TCAGCACGTG AAGCTGCAGC TGACGACAGG
    GCGGGCAGTG ATGGAATGAG CATAATTGAC AGAGGTCGTT TGGAACAAAT GGATGTGAGT
    GAAACAGAGA CTGGAGGAAT TCCTACGGAA GAAAACCAAG CAGCAGAATC AGGTGGTGCT
    GAGGATGCTG TGGCTGCAGA GGCCATGAAG GAACCTCCCG CAGGAGAAGA GGGTTTCAGA
    AGGCAGCACA GCACCTGGTC TGGGCAGCAG ACAAGCTCAG GGACAGCAGT GCAGGACAAG
    GCCCATCTGC AGGAGCCAGC TGAAGACCTC CATAACACCA TGGCAGGCAT TGAGAGCCGC
    AGTGCTCCTC GTGCTGGGAG CGCCTTCGAT GGGAGCTGCC TCAACCTGCC GCGCTTTGTT
    CAGCTCATGG AGACGTTCCT CGGGGAAGGC ATGGCTCTGC CTGCCGTGGA GGGGCTCATT
    GGGTTCATCA GAGAGGAATA CAAACAGACC GAAGAGGAAA AAGTGAAACA ACTAGAAAAA
    GCACATCACA GCTCCCGTTT GACCCGACGG GAACTGCTTC TGGAAGCACT GTTTGAAAAG
    TGGGACATCA ACGCGTCCGG GTTTCTGGAG ATGAAAGAGG TGGAGGCTGC TCTGAGTGCA
    TTCAAGGAAG GAATGGAAAA AGAGGCCGTG AGGATGGCAA AGAACCACTT TTGCTCACGT
    TACCCTCAGC TTGGCAGAGT GGGGAAGCTA TCCCCAAAGG CATTCCAGGC TTTCCTCGAG
    TTGGTGGCTT CTGAGCTCAC TGGGGATGAA GATGAAGTCA TTGATAACAT CGTGGAGTTT
    CTGACCACAA GAGTGGAACG AAGTCACACG GAGTGCTTGC AAAGCTTGGC GAGGAGGAGA
    TGGCTGCACA GCATCCAGCA GGCGGCCGAG AGGAGCGGAG CACGCATGGA GCCGGTGTAC
    GACGCGGTGC TCAAGGCCCT CCTCAAGGAT GCAGAAGCCC ACGGGGATAA CAAGAAGATC
    AGTGCATATA TTGCCCTTTT GGAAGAAAAC CAGCTCTCAC CAGAGCGGGG ACAGACGCTC
    TTACGCTATG TGACATGCAC CACAGATGAT GCTCCGTATG TTCTAAACCA GATACTCTAC
    AGAGACATGA AAGGAGTAAG CTTTGCAGCT GTAGAAGAGG AAAAGCCAAT CCATGTGCCA
    AGAGTTCAGC TGCATGGAAA TATCCACTTC TGGAACCAGG ACCGCCCAGC TGAGCAGAGG
    AGAGGCTCAT TCCTGGTACT GCCATTGCAG GATGCTCACT GGAGAGTCTT TGGCACCCTG
    GGACTTGATA CCCTTCGGGA CCCAAGTGAG AAAACCATCT TCCTGACCCA CGAGATCAGC
    TTCTACCAGG GAGTTTCTCA TGCATTCAGC AAAGCCTACC ACCATGTGTG TGCACTGGGA
    AGTGTTCTGC AGATGGCCCT CACTGCTCTG GACTGGTTGT ATCACAGAGT GCCCAGCATC
    CACACTGTCA GTACATACCT GGTGGAGCCT GGCAACGATC AGACGTGTGA CTATGCTCTG
    CGCAGAATGG CTACCACAGA CAGTACAGGA CAAAAGGAAA TCCATACCTC TCCTGTCCTC
    CTCCTCAGAA AAGAGAACCT CTTCAGAGAT TACCTGTTTA AGTGCACAGA CTGCTCAGAG
    GTCGTTCACA GTTCTGTCTG TGGAGAGCAG CACATTGCAG TGCCTCTCCG GGACCTGTCG
    GGACGAGCCC TGGGGTTGTT TGATATCAGC ATCGGACACC ACCAGGCATT ACCTCCCCAC
    GAACGTAAGG ACCTGCAGAG AATGCTGAAG ATGGCCCAAG CTGCCTGCTC AGAGATACTG
    AAGATGCCTT CAGAGGAAAC CGAAGCAAGC CGTGTCCTGG AAGCAGAACA CAGGAATGTG
    AGGCATGCAG GGATTCTCTT CCACCGGTTC ATGCTGCAGG ACCTGCGTGA GCGTATCCAG
    AAACTGAATG CTGAGAGCTT TGCTGAAATC AAGAGCTATG AAGACCCACC AGCTCTGGTC
    CATAACATAA TGAAGGCCGT GCTGTTGCTT TTGCATCCAG AGTGGGAGGG CACGGAGAAG
    ACTGAGAAGT GGAATCAGTG CATACTGCAA TTTGACGACG ATTTAATTCA GGAGATGTAT
    TGCTTTGATC CAACTGCTGC ATCTGTGCAC GTTCCAGCAG AGATGGTGCA GGACTGTATC
    AATGGGGCGC CTCAGGCAGC AGCGTGGCCA CGGGGCTCCA CCACAGCCCG GCTCCTGGAG
    CTCTGGGCAC ACACCTGCCT GTGCCTGGCC CAGCTGGCAG AGAGCATGCA GAGCAACACG
    GCCCCGTCCT GCAGCGCTGC CCACAGCAAC GCCTCCTGTC AGACCCCCGT CTGCTCCCCC
    AGCCCCAGTG TTCCCCATAT TGGACGCCTG TTTCATAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17249
    Clone ID Related Accession (Same CDS sequence) XM_015296107.1 , XM_015296107.2
    Accession Version XM_015296107.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAAGTAG AAAGGAAGGA CGTGCTTGCA GCAGCCGAGG AGCACGCCAG GCTGGACCCC 
    ATTACTTTTC TTGGAGAATA TCTGATGAGG CATAACCCGC AGTATGACAC CTCTACCAAC
    CCAGGGCCCT ACCTGAGGGG ACTGAAGGCG GCCACAGAGG AGCTAAGAAG GGAGATCCCA
    GATACGGCAT CGGAGAGGCT GGCCAGGATG AAGTCTGAGG CAAAAACCAA GCGTAAGGAA
    AGAGAACAGG CAGAAAGGAT GAAGTGCCAG CAGAAGGAGA TGAGAAAAGC AGCACTGGCA
    ACTCAGTTCA AAGAATGGAC AGCGGATGTC AGTGGCAGAA TTCCAGTTGC ACTGATTCAG
    AGTGCACTGA GGATTTTTCT GGATGTCTGT GCTTCTATGC CCGTAGACAC AAGAAAAGCC
    TATGAGAGGA AGCTGGAAAT CACAGACATG TGGGGGGAAA ACGTAAATGC TGATGGATTC
    ATAGAGTATG TCCATTCCTA CACGGAGAAC TTTCCAAATG ATGCATTCCA GGAGGTCCTG
    AAGCACCTCG GTCAGTGTGC TGCTGACTTC CAAGAATCCA TCAGGCATAA TACGTGCAGG
    CAGATGTTTA TTGACCTCTT CCTACACTGT GATCGAGGAC AGGTTGGTGT CTTGGATAGA
    CCCCAAATAC TTGACCTGCT GGAGCAGTTC TATGAGAGGA GCTCTGTGTG TGCCAGGAGG
    AGATTCTGCA ACCCCAAAGA GTGGCCAATA GTCGAGCTGC AGGAGGTCGG TCTGGAGGAT
    CTGTGGGGAG GCCTTGAGGA CCAGCAAGGT TATGAGGAGC CTGGAGAAGG TTTGTTTTAT
    TCTCAAGCAG AGATTTCTGA GAGAGACGTT TCAGCACGTG AAGCTGCAGC TGACGACAGG
    GCGGGCAGTG ATGGAATGAG CATAATTGAC AGAGGTCGTT TGGAACAAAT GGATGTGAGT
    GAAACAGAGA CTGGAGGAAT TCCTACGGAA GAAAACCAAG CAGCAGAATC AGGTGGTGCT
    GAGGATGCTG TGGCTGCAGA GGCCATGAAG GAACCTCCCG CAGGAGAAGA GGGTTTCAGA
    AGGCAGCACA GCACCTGGTC TGGGCAGCAG ACAAGCTCAG GGACAGCAGT GCAGGACAAG
    GCCCATCTGC AGGAGCCAGC TGAAGACCTC CATAACACCA TGGCAGGCAT TGAGAGCCGC
    AGTGCTCCTC GTGCTGGGAG CGCCTTCGAT GGGAGCTGCC TCAACCTGCC GCGCTTTGTT
    CAGCTCATGG AGACGTTCCT CGGGGAAGGC ATGGCTCTGC CTGCCGTGGA GGGGCTCATT
    GGGTTCATCA GAGAGGAATA CAAACAGACC GAAGAGGAAA AAGTGAAACA ACTAGAAAAA
    GCACATCACA GCTCCCGTTT GACCCGACGG GAACTGCTTC TGGAAGCACT GTTTGAAAAG
    TGGGACATCA ACGCGTCCGG GTTTCTGGAG ATGAAAGAGG TGGAGGCTGC TCTGAGTGCA
    TTCAAGGAAG GAATGGAAAA AGAGGCCGTG AGGATGGCAA AGAACCACTT TTGCTCACGT
    TACCCTCAGC TTGGCAGAGT GGGGAAGCTA TCCCCAAAGG CATTCCAGGC TTTCCTCGAG
    TTGGTGGCTT CTGAGCTCAC TGGGGATGAA GATGAAGTCA TTGATAACAT CGTGGAGTTT
    CTGACCACAA GAGTGGAACG AAGTCACACG GAGTGCTTGC AAAGCTTGGC GAGGAGGAGA
    TGGCTGCACA GCATCCAGCA GGCGGCCGAG AGGAGCGGAG CACGCATGGA GCCGGTGTAC
    GACGCGGTGC TCAAGGCCCT CCTCAAGGAT GCAGAAGCCC ACGGGGATAA CAAGAAGATC
    AGTGCATATA TTGCCCTTTT GGAAGAAAAC CAGCTCTCAC CAGAGCGGGG ACAGACGCTC
    TTACGCTATG TGACATGCAC CACAGATGAT GCTCCGTATG TTCTAAACCA GATACTCTAC
    AGAGACATGA AAGGAGTAAG CTTTGCAGCT GTAGAAGAGG AAAAGCCAAT CCATGTGCCA
    AGAGTTCAGC TGCATGGAAA TATCCACTTC TGGAACCAGG ACCGCCCAGC TGAGCAGAGG
    AGAGGCTCAT TCCTGGTACT GCCATTGCAG GATGCTCACT GGAGAGTCTT TGGCACCCTG
    GGACTTGATA CCCTTCGGGA CCCAAGTGAG AAAACCATCT TCCTGACCCA CGAGATCAGC
    TTCTACCAGG GAGTTTCTCA TGCATTCAGC AAAGCCTACC ACCATGTGTG TGCACTGGGA
    AGTGTTCTGC AGATGGCCCT CACTGCTCTG GACTGGTTGT ATCACAGAGT GCCCAGCATC
    CACACTGTCA GTACATACCT GGTGGAGCCT GGCAACGATC AGACGTGTGA CTATGCTCTG
    CGCAGAATGG CTACCACAGA CAGTACAGGA CAAAAGGAAA TCCATACCTC TCCTGTCCTC
    CTCCTCAGAA AAGAGAACCT CTTCAGAGAT TACCTGTTTA AGTGCACAGA CTGCTCAGAG
    GTCGTTCACA GTTCTGTCTG TGGAGAGCAG CACATTGCAG TGCCTCTCCG GGACCTGTCG
    GGACGAGCCC TGGGGTTGTT TGATATCAGC ATCGGACACC ACCAGGCATT ACCTCCCCAC
    GAACGTAAGG ACCTGCAGAG AATGCTGAAG ATGGCCCAAG CTGCCTGCTC AGAGATACTG
    AAGATGCCTT CAGAGGAAAC CGAAGCAAGC CGTGTCCTGG AAGCAGAACA CAGGAATGTG
    AGGCATGCAG GGATTCTCTT CCACCGGTTC ATGCTGCAGG ACCTGCGTGA GCGTATCCAG
    AAACTGAATG CTGAGAGCTT TGCTGAAATC AAGAGCTATG AAGACCCACC AGCTCTGGTC
    CATAACATAA TGAAGGCCGT GCTGTTGCTT TTGCATCCAG AGTGGGAGGG CACGGAGAAG
    ACTGAGAAGT GGAATCAGTG CATACTGCAA TTTGACGACG ATTTAATTCA GGAGATGTAT
    TGCTTTGATC CAACTGCTGC ATCTGTGCAC GTTCCAGCAG AGATGGTGCA GGACTGTATC
    AATGGGGCGC CTCAGGCAGC AGCGTGGCCA CGGGGCTCCA CCACAGCCCG GCTCCTGGAG
    CTCTGGGCAC ACACCTGCCT GTGCCTGGCC CAGCTGGCAG AGAGCATGCA GAGCAACACG
    GCCCCGTCCT GCAGCGCTGC CCACAGCAAC GCCTCCTGTC AGACCCCCGT CTGCTCCCCC
    AGCCCCAGTG TTCCCCATAT TGGACGCCTG TTTCATAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015151593.1
    CDS3397..6678
    Translation

    Target ORF information:

    RefSeq Version XM_015296107.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296107.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAAGTAG AAAGGAAGGA CGTGCTTGCA GCAGCCGAGG AGCACGCCAG GCTGGACCCC 
    ATTACTTTTC TTGGAGAATA TCTGATGAGG CATAACCCGC AGTATGACAC CTCTACCAAC
    CCAGGGCCCT ACCTGAGGGG ACTGAAGGCG GCCACAGAGG AGCTAAGAAG GGAGATCCCA
    GATACGGCAT CGGAGAGGCT GGCCAGGATG AAGTCTGAGG CAAAAACCAA GCGTAAGGAA
    AGAGAACAGG CAGAAAGGAT GAAGTGCCAG CAGAAGGAGA TGAGAAAAGC AGCACTGGCA
    ACTCAGTTCA AAGAATGGAC AGCGGATGTC AGTGGCAGAA TTCCAGTTGC ACTGATTCAG
    AGTGCACTGA GGATTTTTCT GGATGTCTGT GCTTCTATGC CCGTAGACAC AAGAAAAGCC
    TATGAGAGGA AGCTGGAAAT CACAGACATG TGGGGGGAAA ACGTAAATGC TGATGGATTC
    ATAGAGTATG TCCATTCCTA CACGGAGAAC TTTCCAAATG ATGCATTCCA GGAGGTCCTG
    AAGCACCTCG GTCAGTGTGC TGCTGACTTC CAAGAATCCA TCAGGCATAA TACGTGCAGG
    CAGATGTTTA TTGACCTCTT CCTACACTGT GATCGAGGAC AGGTTGGTGT CTTGGATAGA
    CCCCAAATAC TTGACCTGCT GGAGCAGTTC TATGAGAGGA GCTCTGTGTG TGCCAGGAGG
    AGATTCTGCA ACCCCAAAGA GTGGCCAATA GTCGAGCTGC AGGAGGTCGG TCTGGAGGAT
    CTGTGGGGAG GCCTTGAGGA CCAGCAAGGT TATGAGGAGC CTGGAGAAGG TTTGTTTTAT
    TCTCAAGCAG AGATTTCTGA GAGAGACGTT TCAGCACGTG AAGCTGCAGC TGACGACAGG
    GCGGGCAGTG ATGGAATGAG CATAATTGAC AGAGGTCGTT TGGAACAAAT GGATGTGAGT
    GAAACAGAGA CTGGAGGAAT TCCTACGGAA GAAAACCAAG CAGCAGAATC AGGTGGTGCT
    GAGGATGCTG TGGCTGCAGA GGCCATGAAG GAACCTCCCG CAGGAGAAGA GGGTTTCAGA
    AGGCAGCACA GCACCTGGTC TGGGCAGCAG ACAAGCTCAG GGACAGCAGT GCAGGACAAG
    GCCCATCTGC AGGAGCCAGC TGAAGACCTC CATAACACCA TGGCAGGCAT TGAGAGCCGC
    AGTGCTCCTC GTGCTGGGAG CGCCTTCGAT GGGAGCTGCC TCAACCTGCC GCGCTTTGTT
    CAGCTCATGG AGACGTTCCT CGGGGAAGGC ATGGCTCTGC CTGCCGTGGA GGGGCTCATT
    GGGTTCATCA GAGAGGAATA CAAACAGACC GAAGAGGAAA AAGTGAAACA ACTAGAAAAA
    GCACATCACA GCTCCCGTTT GACCCGACGG GAACTGCTTC TGGAAGCACT GTTTGAAAAG
    TGGGACATCA ACGCGTCCGG GTTTCTGGAG ATGAAAGAGG TGGAGGCTGC TCTGAGTGCA
    TTCAAGGAAG GAATGGAAAA AGAGGCCGTG AGGATGGCAA AGAACCACTT TTGCTCACGT
    TACCCTCAGC TTGGCAGAGT GGGGAAGCTA TCCCCAAAGG CATTCCAGGC TTTCCTCGAG
    TTGGTGGCTT CTGAGCTCAC TGGGGATGAA GATGAAGTCA TTGATAACAT CGTGGAGTTT
    CTGACCACAA GAGTGGAACG AAGTCACACG GAGTGCTTGC AAAGCTTGGC GAGGAGGAGA
    TGGCTGCACA GCATCCAGCA GGCGGCCGAG AGGAGCGGAG CACGCATGGA GCCGGTGTAC
    GACGCGGTGC TCAAGGCCCT CCTCAAGGAT GCAGAAGCCC ACGGGGATAA CAAGAAGATC
    AGTGCATATA TTGCCCTTTT GGAAGAAAAC CAGCTCTCAC CAGAGCGGGG ACAGACGCTC
    TTACGCTATG TGACATGCAC CACAGATGAT GCTCCGTATG TTCTAAACCA GATACTCTAC
    AGAGACATGA AAGGAGTAAG CTTTGCAGCT GTAGAAGAGG AAAAGCCAAT CCATGTGCCA
    AGAGTTCAGC TGCATGGAAA TATCCACTTC TGGAACCAGG ACCGCCCAGC TGAGCAGAGG
    AGAGGCTCAT TCCTGGTACT GCCATTGCAG GATGCTCACT GGAGAGTCTT TGGCACCCTG
    GGACTTGATA CCCTTCGGGA CCCAAGTGAG AAAACCATCT TCCTGACCCA CGAGATCAGC
    TTCTACCAGG GAGTTTCTCA TGCATTCAGC AAAGCCTACC ACCATGTGTG TGCACTGGGA
    AGTGTTCTGC AGATGGCCCT CACTGCTCTG GACTGGTTGT ATCACAGAGT GCCCAGCATC
    CACACTGTCA GTACATACCT GGTGGAGCCT GGCAACGATC AGACGTGTGA CTATGCTCTG
    CGCAGAATGG CTACCACAGA CAGTACAGGA CAAAAGGAAA TCCATACCTC TCCTGTCCTC
    CTCCTCAGAA AAGAGAACCT CTTCAGAGAT TACCTGTTTA AGTGCACAGA CTGCTCAGAG
    GTCGTTCACA GTTCTGTCTG TGGAGAGCAG CACATTGCAG TGCCTCTCCG GGACCTGTCG
    GGACGAGCCC TGGGGTTGTT TGATATCAGC ATCGGACACC ACCAGGCATT ACCTCCCCAC
    GAACGTAAGG ACCTGCAGAG AATGCTGAAG ATGGCCCAAG CTGCCTGCTC AGAGATACTG
    AAGATGCCTT CAGAGGAAAC CGAAGCAAGC CGTGTCCTGG AAGCAGAACA CAGGAATGTG
    AGGCATGCAG GGATTCTCTT CCACCGGTTC ATGCTGCAGG ACCTGCGTGA GCGTATCCAG
    AAACTGAATG CTGAGAGCTT TGCTGAAATC AAGAGCTATG AAGACCCACC AGCTCTGGTC
    CATAACATAA TGAAGGCCGT GCTGTTGCTT TTGCATCCAG AGTGGGAGGG CACGGAGAAG
    ACTGAGAAGT GGAATCAGTG CATACTGCAA TTTGACGACG ATTTAATTCA GGAGATGTAT
    TGCTTTGATC CAACTGCTGC ATCTGTGCAC GTTCCAGCAG AGATGGTGCA GGACTGTATC
    AATGGGGCGC CTCAGGCAGC AGCGTGGCCA CGGGGCTCCA CCACAGCCCG GCTCCTGGAG
    CTCTGGGCAC ACACCTGCCT GTGCCTGGCC CAGCTGGCAG AGAGCATGCA GAGCAACACG
    GCCCCGTCCT GCAGCGCTGC CCACAGCAAC GCCTCCTGTC AGACCCCCGT CTGCTCCCCC
    AGCCCCAGTG TTCCCCATAT TGGACGCCTG TTTCATAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17250
    Clone ID Related Accession (Same CDS sequence) XM_004946639.3 , XM_004946639.2
    Accession Version XM_004946639.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006106.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004946639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGGTATTGTG
    CACACAGAGC CAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004946696.1
    CDS3140..6277
    Translation

    Target ORF information:

    RefSeq Version XM_004946639.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004946639.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGGTATTGTG
    CACACAGAGC CAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa17250
    Clone ID Related Accession (Same CDS sequence) XM_004946639.3 , XM_004946639.2
    Accession Version XM_004946639.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455988.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGGTATTGTG
    CACACAGAGC CAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004946696.1
    CDS1639..4776
    Translation

    Target ORF information:

    RefSeq Version XM_004946639.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus EF-hand calcium binding domain 5 (EFCAB5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004946639.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGCTC AGCCAGAAGA AATGGCCCAA GATGGAGGTC TGAAGCGTCC CAAAGCTGGA 
    GCTTTGTGTG GTGTGACTGC CTCAGAAATG GAGAGAAAGC CATGGGGTGC TGAGCTGCCA
    CGTCTCAGCC GTCACGCTCC GTGGGAAGGG TGCTTTTATG ACAAGGTGCA GCAGAGGACC
    CTGAGTCTGC AGGAGGAGAA GGTGAGGACG ATTCGTGCAC GGAAGGCGGA AGAAGAAAAG
    GTAACAAAGA GAGAGCCCTG TGATGGGCTG TGCCAGGAGT GGCTCAGTAA TGAGGAGGAG
    TCTCTGGATG CTCGCACCTA TTTGACTGAC AAACTCCTAC CAACGTTAAT CCCAGGAGTG
    GAAAAGCTGT TAATGGAAGT AGAAAGGAAG GACGTGCTTG CAGCAGCCGA GGAGCACGCC
    AGGCTGGACC CCATTACTTT TCTTGGAGAA TATCTGATGA GGCATAACCC GCAGTATGAC
    ACCTCTACCA ACCCAGGGCC CTACCTGAGG GGACTGAAGG CGGCCACAGA GGAGCTAAGA
    AGGGAGATCC CAGATACGGC ATCGGAGAGG CTGGCCAGGA TGAAGTCTGA GGCAAAAACC
    AAGCGTAAGG AAAGAGAACA GGCAGAAAGG ATGAAGTGCC AGCAGAAGGA GATGAGAAAA
    GCAGCACTGG CAACTCAGTT CAAAGAATGG ACAGCGGATG TCAGTGGCAG AATTCCAGTT
    GCACTGATTC AGAGTGCACT GAGGATTTTT CTGGATGTCT GTGCTTCTAT GCCCGTAGAC
    ACAAGAAAAG CCTATGAGAG GAAGCTGGAA ATCACAGACA TGTGGGGGGA AAACGTAAAT
    GCTGATGGAT TCATAGAGTA TGTCCATTCC TACACGGAGA ACTTTCCAAA TGATGCATTC
    CAGGAGGTCC TGAAGCACCT CGGTCAGTGT GCTGCTGACT TCCAAGAATC CATCAGGCAT
    AATACGTGCA GGCAGATGTT TATTGACCTC TTCCTACACT GTGATCGAGG ACAGGTTGGT
    GTCTTGGATA GACCCCAAAT ACTTGACCTG CTGGAGCAGT TCTATGAGAG GAGCTCTGTG
    TGTGCCAGGA GGAGATTCTG CAACCCCAAA GAGTGGCCAA TAGTCGAGCT GCAGGAGGTC
    GGTCTGGAGG ATCTGTGGGG AGGCCTTGAG GACCAGCAAG GTTATGAGGA GCCTGGAGAA
    GGTTTGTTTT ATTCTCAAGC AGAGATTTCT GAGAGAGACG TTTCAGCACG TGAAGCTGCA
    GCTGACGACA GGGCGGGCAG TGATGGAATG AGCATAATTG ACAGAGGTCG TTTGGAACAA
    ATGGATGTGA GTGAAACAGA GACTGGAGGA ATTCCTACGG AAGAAAACCA AGCAGCAGAA
    TCAGGTGGTG CTGAGGATGC TGTGGCTGCA GAGGCCATGA AGGAACCTCC CGCAGGAGAA
    GAGGGTTTCA GAAGGCAGCA CAGCACCTGG TCTGGGCAGC AGACAAGCTC AGGGACAGCA
    GTGCAGGACA AGGCCCATCT GCAGGAGCCA GCTGAAGACC TCCATAACAC CATGGCAGGC
    ATTGAGAGCC GCAGTGCTCC TCGTGCTGGG AGCGCCTTCG ATGGGAGCTG CCTCAACCTG
    CCGCGCTTTG TTCAGCTCAT GGAGACGTTC CTCGGGGAAG GCATGGCTCT GCCTGCCGTG
    GAGGGGCTCA TTGGGTTCAT CAGAGAGGAA TACAAACAGA CCGAAGAGGA AAAAGTGAAA
    CAACTAGAAA AAGCACATCA CAGCTCCCGT TTGACCCGAC GGGAACTGCT TCTGGAAGCA
    CTGTTTGAAA AGTGGGACAT CAACGCGTCC GGGTTTCTGG AGATGAAAGA GGTGGAGGCT
    GCTCTGAGTG CATTCAAGGA AGGAATGGAA AAAGAGGCCG TGAGGATGGC AAAGAACCAC
    TTTTGCTCAC GTTACCCTCA GCTTGGCAGA GTGGGGAAGC TATCCCCAAA GGCATTCCAG
    GCTTTCCTCG AGTTGGTGGC TTCTGAGCTC ACTGGGGATG AAGATGAAGT CATTGATAAC
    ATCGTGGAGT TTCTGACCAC AAGAGTGGAA CGAAGTCACA CGGAGTGCTT GCAAAGCTTG
    GCGAGGAGGA GATGGCTGCA CAGCATCCAG CAGGCGGCCG AGAGGAGCGG AGCACGCATG
    GAGCCGGTGT ACGACGCGGT GCTCAAGGCC CTCCTCAAGG ATGCAGAAGC CCACGGGGAT
    AACAAGAAGA TCAGTGCATA TATTGCCCTT TTGGAAGAAA ACCAGCTCTC ACCAGAGCGG
    GGACAGACGC TCTTACGCTA TGTGACATGC ACCACAGATG ATGCTCCGTA TGTTCTAAAC
    CAGATACTCT ACAGAGACAT GAAAGGAGTA AGCTTTGCAG CTGTAGAAGA GGAAAAGCCA
    ATCCATGTGC CAAGAGTTCA GCTGCATGGA AATATCCACT TCTGGAACCA GGACCGCCCA
    GCTGAGCAGA GGAGAGGCTC ATTCCTGGTA CTGCCATTGC AGGATGCTCA CTGGAGAGTC
    TTTGGCACCC TGGGACTTGA TACCCTTCGG GACCCAAGTG AGAAAACCAT CTTCCTGACC
    CACGAGATCA GCTTCTACCA GGGAGTTTCT CATGCATTCA GCAAAGCCTA CCACCATGTG
    TGTGCACTGG GAAGTGTTCT GCAGATGGCC CTCACTGCTC TGGACTGGTT GTATCACAGA
    GTGCCCAGCA TCCACACTGT CAGTACATAC CTGGTGGAGC CTGGCAACGA TCAGACGTGT
    GACTATGCTC TGCGCAGAAT GGCTACCACA GACAGTACAG GACAAAAGGA AATCCATACC
    TCTCCTGTCC TCCTCCTCAG AAAAGAGAAC CTCTTCAGAG ATTACCTGTT TAAGTGCACA
    GACTGCTCAG AGGTCGTTCA CAGTTCTGTC TGTGGAGAGC AGCACATTGC AGTGCCTCTC
    CGGGACCTGT CGGGACGAGC CCTGGGGTTG TTTGATATCA GCATCGGACA CCACCAGGCA
    TTACCTCCCC ACGAACGTAA GGACCTGCAG AGAATGCTGA AGATGGCCCA AGCTGCCTGC
    TCAGAGATAC TGAAGATGCC TTCAGAGGAA ACCGAAGCAA GCCGTGTCCT GGGTATTGTG
    CACACAGAGC CAGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.