S100B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	S100B
Entrez Gene ID	525716
Full Name	S100 calcium binding protein B
General protein information	Preferred Names S100 calcium binding protein B Names protein S100-B S-100 protein beta chain S-100 protein subunit beta S100 protein, beta polypeptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034555.3	NP_001029727.1	protein S100-B

Pathways from BioSystems

Homologies

Homo sapiens (human)	S100B	NP_006263.1
Bos taurus (cattle)	S100B	NP_001029727.1
Pan troglodytes (chimpanzee)	S100B	XP_531586.1
Canis lupus familiaris (dog)	S100B	XP_548737.3
Rattus norvegicus (Norway rat)	S100b	NP_037323.1
Gallus gallus (chicken)	S100B	XP_001233035.1
	S100B	XP_001098016.1
Mus musculus (house mouse)	S100b	NP_033141.1
Danio rerio (zebrafish)	s100b	NP_001002552.1

Gene Ontology
Bibliography

Related articles in PubMed

Evaluation of S100B in cerebrospinal fluid as a potential biomarker for neurological diseases in calves.
Kojima Y, Chiba S, Horiuchi N, Kobayashi Y, Inokuma H
The Journal of veterinary medical science77(5)605-7(2015 May)

cAMP ameliorates inflammation by modulation of macrophage receptor for advanced glycation end-products.
Motoyoshi S, Yamamoto Y, Munesue S, Igawa H, Harashima A, Saito H, Han D, Watanabe T, Sato H, Yamamoto H
The Biochemical journal463(1)75-82(2014 Oct)

S100B protein stimulates microglia migration via RAGE-dependent up-regulation of chemokine expression and release.
Bianchi R, Kastrisianaki E, Giambanco I, Donato R
The Journal of biological chemistry286(9)7214-26(2011 Mar)

Current inhibition of human EAG1 potassium channels by the Ca2+ binding protein S100B.
Sahoo N, Tr?ger J, Heinemann SH, Sch?nherr R
FEBS letters584(18)3896-900(2010 Sep)

S100B protein in myoblasts modulates myogenic differentiation via NF-kappaB-dependent inhibition of MyoD expression.
Tubaro C, Arcuri C, Giambanco I, Donato R
Journal of cellular physiology223(1)270-82(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

As CSF-S100B levels in calves with neurologic diseases widely differed, the utility of CSF-S100B as a diagnostic marker for neurologic diseases in cattle remains inconclusive.
Title: Evaluation of S100B in cerebrospinal fluid as a potential biomarker for neurological diseases in calves.

S100B might participate in the pathophysiology of brain inflammatory disorders via RAGE-dependent regulation of several inflammation-related events including activation and migration of microglia
Title: S100B protein stimulates microglia migration via RAGE-dependent up-regulation of chemokine expression and release.

Intracellular S100B might modulate myoblast differentiation by interfering with MyoD expression in an NF-kappaB-dependent manner.
Title: S100B protein in myoblasts modulates myogenic differentiation via NF-kappaB-dependent inhibition of MyoD expression.

X-ray crystallography was used here to characterize an interaction between Ca(2)(+)-S100B and TRTK-12, a target that binds to the p53-binding site on S100B.
Title: The effects of CapZ peptide (TRTK-12) binding to S100B-Ca2+ as examined by NMR and X-ray crystallography.

Structures of pentamidine (Pnt) bound to Ca(2+)-loaded and Zn(2+),Ca(2+)-loaded S100B were determined by X-ray crystallography at 2.15 A (R(free)=0.266) and 1.85 A (R(free)=0.243) resolution, respectively.
Title: Divalent metal ion complexes of S100B in the absence and presence of pentamidine.

The following S100B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S100B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44901	NM_001034555.3 Latest version!	Bos taurus S100 calcium binding protein B (S100B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

S100B ( NM_001034555.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029727.1 Bos taurus S100 calcium binding protein B (S100B), mRNA.

Price & Availability↑

CloneID	OBa44901
Clone ID Related Accession (Same CDS sequence)	NM_001034555.3
Accession Version	NM_001034555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	protein S100-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103041.1. On Aug 30, 2012 this sequence version replaced NM_001034555.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC103041.1, DV780917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145425, SAMN03145428 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGAGT TAGAGAAGGC CGTGGTGGCC CTCATTGACG TCTTCCATCA ATATTCCGGG 
AGGGAAGGTG ACAAGCACAA GCTGAAGAAG TCAGAACTCA AGGAGCTCAT CAACAATGAA 
CTTTCTCATT TTTTAGAGGA AATCAAAGAG CAGGAGGTTG TGGACAAAGT CATGGAGACA 
CTGGACAGTG ATGGGGACGG CGAATGTGAC TTCCAGGAAT TTATGGCTTT CGTTGCCATG 
ATTACCACTG CCTGCCACGA GTTCTTCGAA CACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029727.1
CDS	54..332
Translation	MSELEKAVVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDSDGDGECDFQEFMAFVAMITTACHEFFEHE

Target ORF information:

RefSeq Version	NM_001034555.3
Organism	Bos taurus(cattle)
Definition	Bos taurus S100 calcium binding protein B (S100B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034555.3

ORF Insert Sequence:

ATGTCTGAGT TAGAGAAGGC CGTGGTGGCC CTCATTGACG TCTTCCATCA ATATTCCGGG 
AGGGAAGGTG ACAAGCACAA GCTGAAGAAG TCAGAACTCA AGGAGCTCAT CAACAATGAA 
CTTTCTCATT TTTTAGAGGA AATCAAAGAG CAGGAGGTTG TGGACAAAGT CATGGAGACA 
CTGGACAGTG ATGGGGACGG CGAATGTGAC TTCCAGGAAT TTATGGCTTT CGTTGCCATG 
ATTACCACTG CCTGCCACGA GTTCTTCGAA CACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluation of S100B in cerebrospinal fluid as a potential biomarker for neurological diseases in calves.
The Journal of veterinary medical science77(5)605-7(2015 May)
Kojima Y,Chiba S,Horiuchi N,Kobayashi Y,Inokuma H

cAMP ameliorates inflammation by modulation of macrophage receptor for advanced glycation end-products.
The Biochemical journal463(1)75-82(2014 Oct)
Motoyoshi S,Yamamoto Y,Munesue S,Igawa H,Harashima A,Saito H,Han D,Watanabe T,Sato H,Yamamoto H

S100B protein stimulates microglia migration via RAGE-dependent up-regulation of chemokine expression and release.
The Journal of biological chemistry286(9)7214-26(2011 Mar)
Bianchi R,Kastrisianaki E,Giambanco I,Donato R

Current inhibition of human EAG1 potassium channels by the Ca2+ binding protein S100B.
FEBS letters584(18)3896-900(2010 Sep)
Sahoo N,Tr?ger J,Heinemann SH,Sch?nherr R

S100B protein in myoblasts modulates myogenic differentiation via NF-kappaB-dependent inhibition of MyoD expression.
Journal of cellular physiology223(1)270-82(2010 Apr)
Tubaro C,Arcuri C,Giambanco I,Donato R