TBXT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBXT
Entrez Gene ID	524443
Full Name	T-box transcription factor T
Synonyms	T
General protein information	Preferred Names T-box transcription factor T Names brachyury protein T brachyury transcription factor T, brachyury homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015472934.1	XP_015328420.1	brachyury protein isoform X1
NM_001192985.1	NP_001179914.1	brachyury protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	T	NP_001003092.1
Rattus norvegicus (Norway rat)	T	NP_001099679.1
Gallus gallus (chicken)	T	NP_990271.1
Danio rerio (zebrafish)	LOC100004296	XP_001343633.3
Mus musculus (house mouse)	T	NP_033335.1
Homo sapiens (human)	T	NP_003172.1
	T	XP_001101514.1
Pan troglodytes (chimpanzee)	T	XP_527563.3
Bos taurus (cattle)	T	NP_001179914.1
Xenopus tropicalis (tropical clawed frog)	t	NP_001008139.1

Gene Ontology
Bibliography

Related articles in PubMed

The mammalian cervical vertebrae blueprint depends on the T (brachyury) gene.
Kromik A, Ulrich R, Kusenda M, Tipold A, Stein VM, Hellige M, Dziallas P, Hadlich F, Widmann P, Goldammer T, Baumg?rtner W, Rehage J, Segelke D, Weikard R, K?hn C
Genetics199(3)873-83(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A spontaneous mutation c.196A > G in T gene was described. This spontaneous vertebral and spinal dysplasia mutation is the first in vivo evidence for T protein involvement in the maintenance of the mammalian seven-cervical vertebra blueprint.
Title: The mammalian cervical vertebrae blueprint depends on the T (brachyury) gene.

The following TBXT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBXT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa36648	NM_001192985.1 Latest version!	Bos taurus T-box transcription factor T (TBXT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa91537	XM_015472934.1 Latest version!	Bos taurus T brachyury transcription factor (T), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBXT ( NM_001192985.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179914.1 Bos taurus T-box transcription factor T (TBXT), mRNA.

Price & Availability↑

CloneID	OBa36648
Clone ID Related Accession (Same CDS sequence)	NM_001192985.1
Accession Version	NM_001192985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	brachyury protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000009.1. On Jul 17, 2010 this sequence version replaced XM_864890.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145415, SAMN03145418 [ECO:0000350] ##Evidence-Data-END##

ATGACCTCCC CGGGCACCGA CAGCCCGGGG AAGAGCCTGC AGTACCGGGT GGACCATCTG 
CTGAGCGCCG TGGAGAGCGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACGGAGCGC 
GAGCTGCGCG TGGGCCTGGA GGAGAGCGAG TTGTGGCTGC GCTTCAAGGA GCTCACCAAC 
GAGATGATCG TCACCAAGAA CGGCAGGAGG ATGTTCCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTAGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC TGACAACCAC 
CGCTGGAAGT ACGTGAATGG GGAGTGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CGCACTGGAT GAAGGCCCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAATG GAGGGGGCCA GATCATGTTG 
AACTCCTTAC ATAAGTATGA GCCTCGAATC CACATCGTGA GAGTTGGGGG TCCACAGCGT 
ATGATCACCA GCCACTGCTT CCCGGAGACC CAGTTCATCG CGGTGACCGC TTACCAAAAT 
GAGGAGATCA CAGCTCTTAA AATTAAATAC AACCCATTTG CAAAAGCTTT CCTCGATGCA 
AAGGAAAGAA GCGATCACAA AGATATGCTG GAAGAAGCAG GAGACGGCCA GCAGTCTGGG 
TACAACCAAT GGGGCTGGCT GATCCCCGGA ACCAGCACCC TGTGTCCACC CGCCACCCCC 
CACCCCCAGT TCGGAGGCCC CCTCTCGCTC CCCTCCACAC ACGGCTGCGA AAGGTACCCG 
GCCCTGAGGA GCCACCGGCC AGCCCCCTAC CCCACCCCGT ACGCGCATCG CAACAGTTCT 
CCAACCTATT CCGACAGTTC ATCTGCGTGT CTGTCCATGC TCCAGCCCCA TGACAACTGG 
TCCAGCCTTG GAATGTCTGC CCACACCAGC ATGCTGCCCA TGGGTCCGAA CGCTGGTCCT 
CCTGCAGGCT CCAGCCAGTA CCCCAGCCTG TGGTCCGTGA GCAGCGCCGC CGTTGCCCCG 
GGCGCCCAGG CGGCGGGCGT GCCCAGCGGG CTGGGAGCCC AGTTCTTTCG AGGCTCCTCC 
GCCCACTCTG CCCCCCTCGC CCACCCGGTC TCGGCATCCT CCTCGTCGGG GTCCCCACTG 
TACGAGGGGG CCGCCACGGC CACAGACGTT GCCGACAGCC AGTACGACGC CTCGGCCCAG 
GCCCGCCTCC TGGCCTCGTG GACGGCCGTG TCGCCCCCGT CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179914.1
CDS	1..1308
Translation	MTSPGTDSPGKSLQYRVDHLLSAVESELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERSDHKDMLEEAGDGQQSGYNQWGWLIPGTSTLCPPATPHPQFGGPLSLPSTHGCERYPALRSHRPAPYPTPYAHRNSSPTYSDSSSACLSMLQPHDNWSSLGMSAHTSMLPMGPNAGPPAGSSQYPSLWSVSSAAVAPGAQAAGVPSGLGAQFFRGSSAHSAPLAHPVSASSSSGSPLYEGAATATDVADSQYDASAQARLLASWTAVSPPSM

Target ORF information:

RefSeq Version	NM_001192985.1
Organism	Bos taurus(cattle)
Definition	Bos taurus T-box transcription factor T (TBXT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192985.1

ORF Insert Sequence:

ATGACCTCCC CGGGCACCGA CAGCCCGGGG AAGAGCCTGC AGTACCGGGT GGACCATCTG 
CTGAGCGCCG TGGAGAGCGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACGGAGCGC 
GAGCTGCGCG TGGGCCTGGA GGAGAGCGAG TTGTGGCTGC GCTTCAAGGA GCTCACCAAC 
GAGATGATCG TCACCAAGAA CGGCAGGAGG ATGTTCCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTAGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC TGACAACCAC 
CGCTGGAAGT ACGTGAATGG GGAGTGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CGCACTGGAT GAAGGCCCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAATG GAGGGGGCCA GATCATGTTG 
AACTCCTTAC ATAAGTATGA GCCTCGAATC CACATCGTGA GAGTTGGGGG TCCACAGCGT 
ATGATCACCA GCCACTGCTT CCCGGAGACC CAGTTCATCG CGGTGACCGC TTACCAAAAT 
GAGGAGATCA CAGCTCTTAA AATTAAATAC AACCCATTTG CAAAAGCTTT CCTCGATGCA 
AAGGAAAGAA GCGATCACAA AGATATGCTG GAAGAAGCAG GAGACGGCCA GCAGTCTGGG 
TACAACCAAT GGGGCTGGCT GATCCCCGGA ACCAGCACCC TGTGTCCACC CGCCACCCCC 
CACCCCCAGT TCGGAGGCCC CCTCTCGCTC CCCTCCACAC ACGGCTGCGA AAGGTACCCG 
GCCCTGAGGA GCCACCGGCC AGCCCCCTAC CCCACCCCGT ACGCGCATCG CAACAGTTCT 
CCAACCTATT CCGACAGTTC ATCTGCGTGT CTGTCCATGC TCCAGCCCCA TGACAACTGG 
TCCAGCCTTG GAATGTCTGC CCACACCAGC ATGCTGCCCA TGGGTCCGAA CGCTGGTCCT 
CCTGCAGGCT CCAGCCAGTA CCCCAGCCTG TGGTCCGTGA GCAGCGCCGC CGTTGCCCCG 
GGCGCCCAGG CGGCGGGCGT GCCCAGCGGG CTGGGAGCCC AGTTCTTTCG AGGCTCCTCC 
GCCCACTCTG CCCCCCTCGC CCACCCGGTC TCGGCATCCT CCTCGTCGGG GTCCCCACTG 
TACGAGGGGG CCGCCACGGC CACAGACGTT GCCGACAGCC AGTACGACGC CTCGGCCCAG 
GCCCGCCTCC TGGCCTCGTG GACGGCCGTG TCGCCCCCGT CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBXT ( XM_015472934.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328420.1 Bos taurus T brachyury transcription factor (T), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa91537
Clone ID Related Accession (Same CDS sequence)	XM_015472934.1
Accession Version	XM_015472934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	brachyury protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104261.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC CGGGCACCGA CAGCCCGGGG AAGAGCCTGC AGTACCGGGT GGACCATCTG 
CTGAGCGCCG TGGAGAGCGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACGGAGCGC 
GAGCTGCGCG TGGGCCTGGA GGAGAGCGAG TTGTGGCTGC GCTTCAAGGA GCTCACCAAC 
GAGATGATCG TCACCAAGAA CGGCAGGAGG ATGTTCCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTAGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC TGACAACCAC 
CGCTGGAAGT ACGTGAATGG GGAGTGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CGCACTGGAT GAAGGCCCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAATG GAGGGGGCCA GATCATGTTG 
AACTCCTTAC ATAAGTATGA GCCTCGAATC CACATCGTGA GAGTTGGGGG TCCACAGCGT 
ATGATCACCA GCCACTGCTT CCCGGAGACC CAGTTCATCG CGGTGACCGC TTACCAAAAT 
GAGGAGATCA CAGCTCTTAA AATTAAATAC AACCCATTTG CAAAAGCTTT CCTCGATGCA 
AAGGAAAGAA GCGATCACAA AGATATGCTG GAAGAAGCAG GAGACGGCCA GCAGTCTGGG 
TACAACCAAT CCTATTCCGA CAGTTCATCT GCGTGTCTGT CCATGCTCCA GCCCCATGAC 
AACTGGTCCA GCCTTGGAAT GTCTGCCCAC ACCAGCATGC TGCCCATGGG TCCGAACGCT 
GGTCCTCCTG CAGGCTCCAG CCAGTACCCC AGCCTGTGGT CCGTGAGCAG CGCCGCCGTT 
GCCCCGGGCG CCCAGGCGGC GGGCGTGCCC AGCGGGCTGG GAGCCCAGTT CTTTCGAGGC 
TCCTCCGCCC ACTCTGCCCC CCTCGCCCAC CCGGTCTCGG CATCCTCCTC GTCGGGGTCC 
CCACTGTACG AGGGGGCCGC CACGGCCACA GACGTTGCCG ACAGCCAGTA CGACGCCTCG 
GCCCAGGCCC GCCTCCTGGC CTCGTGGACG GCCGTGTCGC CCCCGTCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328420.1
CDS	1..1134
Translation	MTSPGTDSPGKSLQYRVDHLLSAVESELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERSDHKDMLEEAGDGQQSGYNQSYSDSSSACLSMLQPHDNWSSLGMSAHTSMLPMGPNAGPPAGSSQYPSLWSVSSAAVAPGAQAAGVPSGLGAQFFRGSSAHSAPLAHPVSASSSSGSPLYEGAATATDVADSQYDASAQARLLASWTAVSPPSM

Target ORF information:

RefSeq Version	XM_015472934.1
Organism	Bos taurus(cattle)
Definition	Bos taurus T brachyury transcription factor (T), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472934.1

ORF Insert Sequence:

ATGACCTCCC CGGGCACCGA CAGCCCGGGG AAGAGCCTGC AGTACCGGGT GGACCATCTG 
CTGAGCGCCG TGGAGAGCGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACGGAGCGC 
GAGCTGCGCG TGGGCCTGGA GGAGAGCGAG TTGTGGCTGC GCTTCAAGGA GCTCACCAAC 
GAGATGATCG TCACCAAGAA CGGCAGGAGG ATGTTCCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTAGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC TGACAACCAC 
CGCTGGAAGT ACGTGAATGG GGAGTGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CGCACTGGAT GAAGGCCCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAATG GAGGGGGCCA GATCATGTTG 
AACTCCTTAC ATAAGTATGA GCCTCGAATC CACATCGTGA GAGTTGGGGG TCCACAGCGT 
ATGATCACCA GCCACTGCTT CCCGGAGACC CAGTTCATCG CGGTGACCGC TTACCAAAAT 
GAGGAGATCA CAGCTCTTAA AATTAAATAC AACCCATTTG CAAAAGCTTT CCTCGATGCA 
AAGGAAAGAA GCGATCACAA AGATATGCTG GAAGAAGCAG GAGACGGCCA GCAGTCTGGG 
TACAACCAAT CCTATTCCGA CAGTTCATCT GCGTGTCTGT CCATGCTCCA GCCCCATGAC 
AACTGGTCCA GCCTTGGAAT GTCTGCCCAC ACCAGCATGC TGCCCATGGG TCCGAACGCT 
GGTCCTCCTG CAGGCTCCAG CCAGTACCCC AGCCTGTGGT CCGTGAGCAG CGCCGCCGTT 
GCCCCGGGCG CCCAGGCGGC GGGCGTGCCC AGCGGGCTGG GAGCCCAGTT CTTTCGAGGC 
TCCTCCGCCC ACTCTGCCCC CCTCGCCCAC CCGGTCTCGG CATCCTCCTC GTCGGGGTCC 
CCACTGTACG AGGGGGCCGC CACGGCCACA GACGTTGCCG ACAGCCAGTA CGACGCCTCG 
GCCCAGGCCC GCCTCCTGGC CTCGTGGACG GCCGTGTCGC CCCCGTCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mammalian cervical vertebrae blueprint depends on the T (brachyury) gene.
Genetics199(3)873-83(2015 Mar)
Kromik A,Ulrich R,Kusenda M,Tipold A,Stein VM,Hellige M,Dziallas P,Hadlich F,Widmann P,Goldammer T,Baumg?rtner W,Rehage J,Segelke D,Weikard R,K?hn C

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TBXT cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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