RASGRF1 cDNA ORF clone, Bos taurus(cattle)

The following RASGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa50177 XM_024981793.1
Latest version!
Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa37699 NM_001191457.1
Latest version!
Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa30893 XM_005221879.4
Latest version!
Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa30895 XM_005221881.4
Latest version!
Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa30893 XM_005221879.3 Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa30895 XM_005221881.3 Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa50177
    Clone ID Related Accession (Same CDS sequence) XM_024981793.1
    Accession Version XM_024981793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGATATCC
    CACATCATCC GAGAGATCCG CCAGTTTCAA CAAACTGCCT ACAAGATCGA GCACCAAGCA
    AAGGTAACCC AGTATTTACT GGACCAATCT TTTGTAATGG ATGAAGAAAG CCTCTACGAG
    TCTTCTCTCC GAATAGAACC AAAACTCCCC ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837561.1
    CDS458..4033
    Translation

    Target ORF information:

    RefSeq Version XM_024981793.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGATATCC
    CACATCATCC GAGAGATCCG CCAGTTTCAA CAAACTGCCT ACAAGATCGA GCACCAAGCA
    AAGGTAACCC AGTATTTACT GGACCAATCT TTTGTAATGG ATGAAGAAAG CCTCTACGAG
    TCTTCTCTCC GAATAGAACC AAAACTCCCC ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37699
    Clone ID Related Accession (Same CDS sequence) NM_001191457.1
    Accession Version NM_001191457.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product ras-specific guanine nucleotide-releasing factor 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000021.1. On Jul 17, 2010 this sequence version replaced XM_002702628.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.15208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145416 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA
    GAAGCTCTGA AAAATTGCGA CCCGCCCTGT GTCCCTTACC TGGGGATGTA CCTCACTGAC
    CTGGCCTTCA TCGAAGAGGG TACGCCCAAC TACACGGAGG ACGGCCTGGT CAACTTCTCC
    AAGATGAGAA TGATATCCCA CATCATCCGA GAGATCCGCC AGTTTCAACA AACTGCCTAC
    AAGATCGAGC ACCAAGCAAA GGTAACCCAG TATTTACTGG ACCAATCTTT TGTAATGGAT
    GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178386.1
    CDS1..3774
    Translation

    Target ORF information:

    RefSeq Version NM_001191457.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA
    GAAGCTCTGA AAAATTGCGA CCCGCCCTGT GTCCCTTACC TGGGGATGTA CCTCACTGAC
    CTGGCCTTCA TCGAAGAGGG TACGCCCAAC TACACGGAGG ACGGCCTGGT CAACTTCTCC
    AAGATGAGAA TGATATCCCA CATCATCCGA GAGATCCGCC AGTTTCAACA AACTGCCTAC
    AAGATCGAGC ACCAAGCAAA GGTAACCCAG TATTTACTGG ACCAATCTTT TGTAATGGAT
    GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30893
    Clone ID Related Accession (Same CDS sequence) XM_005221879.3 , XM_005221879.4
    Accession Version XM_005221879.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221879.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA
    GAAGCTCTGA AAAAACAGAT GTTCCACCCA TCTGTGCTGT CCGGCCGTGT TTCCTCCGCC
    CGGGCTCCGC GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCACA TTCACCAACC
    TGTCCGTCCG TCCGTCCGTC CGTCCGCCCC CAGGTGCTGC TCCATCTGCC TCCTTCACAT
    GGCTTCCTCG CCCAGCACCG CCACGGCCTC ACCGCCTCCG TGGTCCCCAT TCTGTCTGCG
    TCCCCATCCT GTCTCCCCTC TTTCAGGTTC CCTGTGGGCT TGTGTAAGTG CCTCCCTAGA
    CGCCTCTTCA CCCAGCCATG CAGGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221936.1
    CDS939..4748
    Translation

    Target ORF information:

    RefSeq Version XM_005221879.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221879.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA
    GAAGCTCTGA AAAAACAGAT GTTCCACCCA TCTGTGCTGT CCGGCCGTGT TTCCTCCGCC
    CGGGCTCCGC GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCACA TTCACCAACC
    TGTCCGTCCG TCCGTCCGTC CGTCCGCCCC CAGGTGCTGC TCCATCTGCC TCCTTCACAT
    GGCTTCCTCG CCCAGCACCG CCACGGCCTC ACCGCCTCCG TGGTCCCCAT TCTGTCTGCG
    TCCCCATCCT GTCTCCCCTC TTTCAGGTTC CCTGTGGGCT TGTGTAAGTG CCTCCCTAGA
    CGCCTCTTCA CCCAGCCATG CAGGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30893
    Clone ID Related Accession (Same CDS sequence) XM_005221879.3 , XM_005221879.4
    Accession Version XM_005221879.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221879.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA
    GAAGCTCTGA AAAAACAGAT GTTCCACCCA TCTGTGCTGT CCGGCCGTGT TTCCTCCGCC
    CGGGCTCCGC GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCACA TTCACCAACC
    TGTCCGTCCG TCCGTCCGTC CGTCCGCCCC CAGGTGCTGC TCCATCTGCC TCCTTCACAT
    GGCTTCCTCG CCCAGCACCG CCACGGCCTC ACCGCCTCCG TGGTCCCCAT TCTGTCTGCG
    TCCCCATCCT GTCTCCCCTC TTTCAGGTTC CCTGTGGGCT TGTGTAAGTG CCTCCCTAGA
    CGCCTCTTCA CCCAGCCATG CAGGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221936.1
    CDS465..4274
    Translation

    Target ORF information:

    RefSeq Version XM_005221879.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221879.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA
    GAAGCTCTGA AAAAACAGAT GTTCCACCCA TCTGTGCTGT CCGGCCGTGT TTCCTCCGCC
    CGGGCTCCGC GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCACA TTCACCAACC
    TGTCCGTCCG TCCGTCCGTC CGTCCGCCCC CAGGTGCTGC TCCATCTGCC TCCTTCACAT
    GGCTTCCTCG CCCAGCACCG CCACGGCCTC ACCGCCTCCG TGGTCCCCAT TCTGTCTGCG
    TCCCCATCCT GTCTCCCCTC TTTCAGGTTC CCTGTGGGCT TGTGTAAGTG CCTCCCTAGA
    CGCCTCTTCA CCCAGCCATG CAGGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30895
    Clone ID Related Accession (Same CDS sequence) XM_005221881.3 , XM_005221881.4
    Accession Version XM_005221881.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGGGTTGG
    TCCAAACCAG GCGACCACAT TTCTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221938.1
    CDS936..4385
    Translation

    Target ORF information:

    RefSeq Version XM_005221881.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221881.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGGGTTGG
    TCCAAACCAG GCGACCACAT TTCTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30895
    Clone ID Related Accession (Same CDS sequence) XM_005221881.3 , XM_005221881.4
    Accession Version XM_005221881.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221881.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGGGTTGG
    TCCAAACCAG GCGACCACAT TTCTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221938.1
    CDS463..3912
    Translation

    Target ORF information:

    RefSeq Version XM_005221881.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221881.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC
    CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG
    GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG
    CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG
    CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC
    AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC
    ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC
    GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC
    AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT
    CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA
    CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC
    ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC
    ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC
    CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT
    GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG
    TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA
    CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT
    GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA
    TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC
    CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC
    CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC
    CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC
    GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA
    TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG
    ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG
    ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC
    CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA
    ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG
    GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG
    AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGGGTTGG
    TCCAAACCAG GCGACCACAT TTCTAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.