RASGRF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF1
Entrez Gene ID	520039
Full Name	Ras protein specific guanine nucleotide releasing factor 1
General protein information	Preferred Names Ras protein specific guanine nucleotide releasing factor 1 Names ras-specific guanine nucleotide-releasing factor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221879.3	XP_005221936.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_024981793.1	XP_024837561.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_005221881.3	XP_005221938.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_005221879.4	XP_005221936.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
NM_001191457.1	NP_001178386.1	ras-specific guanine nucleotide-releasing factor 1
XM_005221881.4	XP_005221938.1	ras-specific guanine nucleotide-releasing factor 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	RASGRF1	NP_002882.3
Mus musculus (house mouse)	Rasgrf1	NP_035375.1
Danio rerio (zebrafish)	si:ch211-234p18.3	XP_002665395.1
Xenopus tropicalis (tropical clawed frog)	rasgrf1	XP_002933009.2
Gallus gallus (chicken)	RASGRF1	XP_004944008.1
Pan troglodytes (chimpanzee)	RASGRF1	XP_001153586.1
Canis lupus familiaris (dog)	RASGRF1	XP_545892.5
Rattus norvegicus (Norway rat)	Rasgrf1	NP_001164002.1
Bos taurus (cattle)	RASGRF1	NP_001178386.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RASGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa50177	XM_024981793.1 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa37699	NM_001191457.1 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa30893	XM_005221879.4 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa30895	XM_005221881.4 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa30893	XM_005221879.3	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa30895	XM_005221881.3	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF1 ( XM_024981793.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837561.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa50177
Clone ID Related Accession (Same CDS sequence)	XM_024981793.1
Accession Version	XM_024981793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGATATCC 
CACATCATCC GAGAGATCCG CCAGTTTCAA CAAACTGCCT ACAAGATCGA GCACCAAGCA 
AAGGTAACCC AGTATTTACT GGACCAATCT TTTGTAATGG ATGAAGAAAG CCTCTACGAG 
TCTTCTCTCC GAATAGAACC AAAACTCCCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837561.1
CDS	458..4033
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEVEIERLKAEIASLLKDNERIQSTQTVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDVDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCSSNGYTSMYSAMSPFGKATLDTSKLYVSSSFANKIPDEGDTATEKSEEPSALSKQSSEVSMREESDNDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDSNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIVRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_024981793.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981793.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGATATCC 
CACATCATCC GAGAGATCCG CCAGTTTCAA CAAACTGCCT ACAAGATCGA GCACCAAGCA 
AAGGTAACCC AGTATTTACT GGACCAATCT TTTGTAATGG ATGAAGAAAG CCTCTACGAG 
TCTTCTCTCC GAATAGAACC AAAACTCCCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( NM_001191457.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178386.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), mRNA.

Price & Availability↑

CloneID	OBa37699
Clone ID Related Accession (Same CDS sequence)	NM_001191457.1
Accession Version	NM_001191457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000021.1. On Jul 17, 2010 this sequence version replaced XM_002702628.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.15208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145416 [ECO:0000350] ##Evidence-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAATTGCGA CCCGCCCTGT GTCCCTTACC TGGGGATGTA CCTCACTGAC 
CTGGCCTTCA TCGAAGAGGG TACGCCCAAC TACACGGAGG ACGGCCTGGT CAACTTCTCC 
AAGATGAGAA TGATATCCCA CATCATCCGA GAGATCCGCC AGTTTCAACA AACTGCCTAC 
AAGATCGAGC ACCAAGCAAA GGTAACCCAG TATTTACTGG ACCAATCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178386.1
CDS	1..3774
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEVEIERLKAEIASLLKDNERIQSTQTVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDVDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCSSNGYTSMYSAMSPFGKATLDTSKLYVSSSFANKIPDEGDTATEKSEEPSALSKQSSEVSMREESDNDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDSNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIVRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	NM_001191457.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191457.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAATTGCGA CCCGCCCTGT GTCCCTTACC TGGGGATGTA CCTCACTGAC 
CTGGCCTTCA TCGAAGAGGG TACGCCCAAC TACACGGAGG ACGGCCTGGT CAACTTCTCC 
AAGATGAGAA TGATATCCCA CATCATCCGA GAGATCCGCC AGTTTCAACA AACTGCCTAC 
AAGATCGAGC ACCAAGCAAA GGTAACCCAG TATTTACTGG ACCAATCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_005221879.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221936.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

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CloneID	OBa30893
Clone ID Related Accession (Same CDS sequence)	XM_005221879.3 , XM_005221879.4
Accession Version	XM_005221879.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221879.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGAT GTTCCACCCA TCTGTGCTGT CCGGCCGTGT TTCCTCCGCC 
CGGGCTCCGC GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCACA TTCACCAACC 
TGTCCGTCCG TCCGTCCGTC CGTCCGCCCC CAGGTGCTGC TCCATCTGCC TCCTTCACAT 
GGCTTCCTCG CCCAGCACCG CCACGGCCTC ACCGCCTCCG TGGTCCCCAT TCTGTCTGCG 
TCCCCATCCT GTCTCCCCTC TTTCAGGTTC CCTGTGGGCT TGTGTAAGTG CCTCCCTAGA 
CGCCTCTTCA CCCAGCCATG CAGGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221936.1
CDS	939..4748
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEVEIERLKAEIASLLKDNERIQSTQTVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDVDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCSSNGYTSMYSAMSPFGKATLDTSKLYVSSSFANKIPDEGDTATEKSEEPSALSKQSSEVSMREESDNDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDSNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIVRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKKQMFHPSVLSGRVSSARAPRAPVDCACGELPPHSPTCPSVRPSVRPQVLLHLPPSHGFLAQHRHGLTASVVPILSASPSCLPSFRFPVGLCKCLPRRLFTQPCRA

Target ORF information:

RefSeq Version	XM_005221879.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221879.3

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGAT GTTCCACCCA TCTGTGCTGT CCGGCCGTGT TTCCTCCGCC 
CGGGCTCCGC GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCACA TTCACCAACC 
TGTCCGTCCG TCCGTCCGTC CGTCCGCCCC CAGGTGCTGC TCCATCTGCC TCCTTCACAT 
GGCTTCCTCG CCCAGCACCG CCACGGCCTC ACCGCCTCCG TGGTCCCCAT TCTGTCTGCG 
TCCCCATCCT GTCTCCCCTC TTTCAGGTTC CCTGTGGGCT TGTGTAAGTG CCTCCCTAGA 
CGCCTCTTCA CCCAGCCATG CAGGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_005221879.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221936.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30893
Clone ID Related Accession (Same CDS sequence)	XM_005221879.3 , XM_005221879.4
Accession Version	XM_005221879.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221879.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGAT GTTCCACCCA TCTGTGCTGT CCGGCCGTGT TTCCTCCGCC 
CGGGCTCCGC GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCACA TTCACCAACC 
TGTCCGTCCG TCCGTCCGTC CGTCCGCCCC CAGGTGCTGC TCCATCTGCC TCCTTCACAT 
GGCTTCCTCG CCCAGCACCG CCACGGCCTC ACCGCCTCCG TGGTCCCCAT TCTGTCTGCG 
TCCCCATCCT GTCTCCCCTC TTTCAGGTTC CCTGTGGGCT TGTGTAAGTG CCTCCCTAGA 
CGCCTCTTCA CCCAGCCATG CAGGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221936.1
CDS	465..4274
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEVEIERLKAEIASLLKDNERIQSTQTVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDVDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCSSNGYTSMYSAMSPFGKATLDTSKLYVSSSFANKIPDEGDTATEKSEEPSALSKQSSEVSMREESDNDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDSNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIVRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKKQMFHPSVLSGRVSSARAPRAPVDCACGELPPHSPTCPSVRPSVRPQVLLHLPPSHGFLAQHRHGLTASVVPILSASPSCLPSFRFPVGLCKCLPRRLFTQPCRA

Target ORF information:

RefSeq Version	XM_005221879.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221879.4

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ACAAGCTCCA AAAGCTTGTG TCATCAGAGG GCAGATTTAA GAATCTGAGA 
GAAGCTCTGA AAAAACAGAT GTTCCACCCA TCTGTGCTGT CCGGCCGTGT TTCCTCCGCC 
CGGGCTCCGC GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCACA TTCACCAACC 
TGTCCGTCCG TCCGTCCGTC CGTCCGCCCC CAGGTGCTGC TCCATCTGCC TCCTTCACAT 
GGCTTCCTCG CCCAGCACCG CCACGGCCTC ACCGCCTCCG TGGTCCCCAT TCTGTCTGCG 
TCCCCATCCT GTCTCCCCTC TTTCAGGTTC CCTGTGGGCT TGTGTAAGTG CCTCCCTAGA 
CGCCTCTTCA CCCAGCCATG CAGGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_005221881.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221938.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30895
Clone ID Related Accession (Same CDS sequence)	XM_005221881.3 , XM_005221881.4
Accession Version	XM_005221881.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGGGTTGG 
TCCAAACCAG GCGACCACAT TTCTAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221938.1
CDS	936..4385
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEVEIERLKAEIASLLKDNERIQSTQTVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDVDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCSSNGYTSMYSAMSPFGKATLDTSKLYVSSSFANKIPDEGDTATEKSEEPSALSKQSSEVSMREESDNDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDSNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIVRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQGWSKPGDHISN

Target ORF information:

RefSeq Version	XM_005221881.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221881.3

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGGGTTGG 
TCCAAACCAG GCGACCACAT TTCTAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_005221881.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221938.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

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CloneID	OBa30895
Clone ID Related Accession (Same CDS sequence)	XM_005221881.3 , XM_005221881.4
Accession Version	XM_005221881.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221881.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGGGTTGG 
TCCAAACCAG GCGACCACAT TTCTAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221938.1
CDS	463..3912
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPTLSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEVEIERLKAEIASLLKDNERIQSTQTVTPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDVDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAVVVLDKLITIYRKPISAIPARSLELLFASGQNNKLLYGDPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCSSNGYTSMYSAMSPFGKATLDTSKLYVSSSFANKIPDEGDTATEKSEEPSALSKQSSEVSMREESDNDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDSNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVISFLEEVMHDPELLTQERKAAANIIRTLTQDDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIVRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQGWSKPGDHISN

Target ORF information:

RefSeq Version	XM_005221881.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221881.4

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
AAGGACGGAA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCTACGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCACT ATTTCACGGT TAACTTCAGC 
CACGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CCAAGGACTG CGACGAGTGG 
GTGGCGGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CGGAGCACGA GGCGCTGATG 
CAAAAATACC TGCACCTGCT GCAGATCGTG GAGACCGAGA AGACGGTGGC CAAGCAGCTG 
CGGCAGCAGA TCGAGGACGG GGAGGTGGAG ATCGAGCGAC TGAAGGCAGA GATCGCATCT 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCACCCCCAA CGACGAAGAC 
AGTGACATCA AAAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT ATGCCGGCGC 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCGC ACGCAGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTACGTGCA GCAGCTGCAC 
ATCCTGGTGA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AGACCATCAT GTTTTTGCAC 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCC 
GACCTGTTCG ACATCCTGCT GCCCATGCTC AACATCTACC AGGAGTTCGT GCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GCGACTTTGA CAAGCTGCTC 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACGCTGCACG AGCTCCTGGC ACACACACCT 
CATGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGGATAATGC ACGATGAAGT CAGTGAGACC GAGAACATCC GGAAAAACCT GGCCATTGAA 
CGCATGATCA TTGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGGTCCCTCA TCCAGGTGCC CATGTCCGAG AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCCCTTTCCC TGAAGAAGGA GGGCGAGAGG CAGTGCTTCC TGTTTTCTAA GCACCTGATC 
ATCTGCACCA GAGGCTCTGG TGGAAAGCTT CACCTGACTA AGAACGGAGT CATATCCCTC 
ATTGACTGCA CCTTGCTGGA GGAACCGGAG AGCACGGAGG AAGAAGCCAA AGGGTCCGGC 
CAAGATGTAG ACCACTTGGA TTTCAAGATC GGGGTGGAGC CAAAGGATTC ACCACCCTTC 
ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCGG CATGGACTAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG GTGCAACGGG CTGATGATGA ACGCCTTTGA GGAGAACTCC 
AAGGTCACAG TGCCACAGAT GATCAAGTCC GACGCTTCCT TGTATTGCGA CGACGTGGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG 
GAGCGGCTAC TGGAGCGACT GACCGATCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CTTACCGCGT CTTCACCACT GCTGTTGTGG TCCTAGACAA GCTCATCACC 
ATCTACAGGA AGCCCATCAG TGCCATCCCC GCCAGGTCAC TGGAGCTCTT GTTTGCCAGT 
GGCCAGAACA ACAAGCTCCT GTACGGGGAC CCACCCAAGT CCCCACGTGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACGTCAT CACCCAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACAGGC GGCAAGGCCC TGGACCTGGC CGCGCTCAGC 
TGCAGCTCCA ACGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTTGG CAAAGCCACA 
CTGGACACCA GCAAGCTCTA CGTGTCCAGC AGCTTTGCCA ACAAGATCCC AGACGAGGGT 
GACACTGCCA CCGAGAAATC CGAAGAGCCC TCTGCCCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGGG AAGAGTCAGA CAATGATCAA AACCAGAGTG ACGATGGTGA CACTGAGACA 
TCCCCCACCA AATCTCCAAC GACACCAAAA TCGGTCAAAA GCAAAAACTC CTCAGAGTTC 
CCCCTCTTCT CCTACAATAA TGGAGTGGTC ATGACCTCCT GTCGCGAGCT GGATAGTAAC 
CGCAGCGCCC TATCAGCAGC CTCTGCCTTC GCGATAGCAA CTGCGGGTGC CAACGAGGGA 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTGGCCA GTGCAGGCTT TCCCCCCGAC 
CAGAGGAACG GAGACAAGGA ATTTGTGATT CGCAGAGCAG CCACCAACCG CGTCCTGAAC 
GTGCTCCGAC ACTGGGTGTC CAAGCATTCT CAGGACTTTG AGACCAATGA CGAGCTCAAA 
TGCAAGGTCA TCAGCTTCCT GGAGGAGGTC ATGCATGACC CAGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CGGCCAACAT CATCAGGACT CTGACCCAGG ATGACCCAGG CGACAACCAG 
ATCACACTCG AGGAGATCAC ACAGATGGCT GAAGGCGTGA AGGCCGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATTGC GGAGCAACTG ACCCTACTGG ATCACCTCGT CTTCAAGAAG 
ATCCCTTATG AGGAGTTCTT CGGACAAGGG TGGATGAAAC TGGAAAAGAA TGAGAGGACC 
CCTTATATCA TGAAAACCAC TAAGCATTTC AATGATATCA GTAACTTGAT CGCTTCAGAA 
ATCGTCCGCA ACGAGGACAT CAACGCGAGG GTGAGCACCA TCGAGAAGTG GGTGGCTGTG 
GCCGACATAT GCCGATGCTT ACACAACTAC AATGCTGTTT TGGAGATCAC CTCGTCCATG 
AACCGGAGCG CGATCTTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGGGTTGG 
TCCAAACCAG GCGACCACAT TTCTAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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RASGRF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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