RASGRF1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following RASGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287266 XM_022417015.1
Latest version!
Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe287267 XM_022417016.1
Latest version!
Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe35657 XM_014112339.1
Latest version!
Canis lupus familiaris Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287266
    Clone ID Related Accession (Same CDS sequence) XM_022417015.1
    Accession Version XM_022417015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTGCGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ATTTCACGGT TAACTTCAGC
    CATGAGAACC AGAAAGCCCT GGAGCTGAGG ACAGAGGATG CCAAGGACTG TGACGAGTGG
    GTGGCAGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CAGAGCACGA AGCGCTGATG
    CAAAAGTATC TGCACCTCCT GCAGATCGTG GAGACAGAGA AGACAGTGGC CAAACAGCTG
    CGGCGGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATTGCATCA
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACGCAGACCG TCACCCCCAA CGATGAAGAC
    AGTGACATCA AGAAGATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGCTCGCCGC ACGCTGATAG CATGCGGAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTATGTGCA GCAGCTCCAC
    ATCCTGGTTA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTCAACAGCG AGACCATCAT GTTCCTGCAC
    CAGATCTTCT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACCCT GGTCCTGGCT
    GACCTGTTCG ACATCCTGCT GCCGATGCTC AACATCTACC AGGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GGGACTTCGA CAAGCTGCTG
    AAGCACTATG AGGCCAAGCC CGACTGCGAG GAACGGACCC TGGAGACCTT CCTCACCTAC
    CCCATGTTTC AGATCCCCAG GTACATCCTG ACGCTGCATG AGCTCCTGGC GCACACCCCT
    CACGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ATGACGAAGT GAGTGAGACA GAGAACATCC GGAAAAACCT CGCCATCGAG
    CGCATGATCA TTGAAGGCTG CGAGATCCTG CTGGACACCA GCCAGACTTT TGTGAGACAA
    GGCTCGCTCA TCCAGGTGCC CATGTCTGAG AAGGGCAAGA TCACCAGGGG ACGCCTGGGG
    TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTCTCCAA GCACCTGATT
    ATCTGCACCA GAGGCTCTGG TGGGAAACTC CACCTGACCA AGAACGGGGT CATCTCCCTC
    ATCGACTGCA CCTTGCTGGA GGAGCCGGAG AGCACAGAGG AAGACGCCAA AGGATCCAGC
    CAAGACATAG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAAGACTC CCCGCCTTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAATGGG CTCATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACTG TGCCACAGAT GATCAAATCC GACGCCTCCT TGTACTGCGA TGACGTAGAC
    ATTCGCTTCA GCAAGACCAT GAACTCGTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GGGCGGCTGC TGGAACGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACG
    TTCCTGCACT CGTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGCTCTG GCCAAGCCTC GAGGGGCCCG
    GTGGGTCACC GTGAGCCCTG GGCCCCTGCC AGATCACTAG AGCTCTTATT CGCCAGCGGC
    CAGAACAACA AGCTTCTGTA TGGTGACCCT CCCAAGTCCC CGCGCGCCAC GCGCAAGTTC
    TCCTCACCGC CACCCCTGTC CATCACCAAG ACGTCATCCC CCAGCCGCCG GCGGAAGCTG
    TCGCTGAACA TCCCCATCAT CACGGGCGGC AAGGCCCTGG ACCTGGCCGC CCTCAGCTGC
    AGCTCCAACG GCTACACCAG CCTGTACTCG GCCGTGTCCC CCTTCAGCAA GGCCACGCTG
    GACACCAGCA AGCTCTTCGT ATCCAGCAGC TTTGCCAACA AGATTCCAGA CGAAGGTGAA
    ACGGCCACTG AGAAGCCCGA AGAGCCCGTG GCTCTCAGCA AGCAGAGCTC AGAAGTCTCC
    GTGAGGGAAG AGTCCGATAA CGATCAAACC CAGAGTGACG ACGGGGACAC TGAGGCGTCC
    CCAACCAAAT CGCCAACAAC GCCAAAGTCA GTCAAAAGCA AAAATTCCTC AGAGTTCCCC
    CTCTTCTCCT ACAACAACGG GGTGGTCATG ACCTCGTGTC GGGAGCTGGA CAACACACGC
    AGCGCCCTGT CCGCGGCCTC TGCCTTTGCC ATAGCCACGG CGGGAGCCAA CGAGGGCACC
    CCGAACAAAG AGAAGTACCG GAGGATGTCC CTGGCCAGCA CAGGGTTTCC CCCTGACCAG
    AGGAACGGGG ACAAGGAGTT CGTGATCCGC AGAGCGGCCA CCAACCGCGT CCTGAACGTG
    CTCCGCCACT GGGTCTCCAA ACACTCGCAG GACTTTGAGA CCAATGACGA GCTGCGATGC
    AAGGTGATCA GCTTCCTGGA GGAGGTCATG CACGACCCAG AGCTGCTGAC CCAGGAGAGG
    AAGGCTGCGG CCAACATTAT CAGGACTCTG ACCCAGGAGG ACCCAGGGGA CAACCAGATC
    ACACTGGAGG AGATCACGCA GATGGCCGAA GGCGTGAAGG CAGAGCCCTT TGAAAACCAC
    TCGGCCCTGG AGATAGCGGA GCAGCTGACC CTGCTGGACC ACCTGGTCTT CAAGAAGATC
    CCTTACGAGG AGTTCTTCGG GCAAGGCTGG ATGAAAGTGG AGAAGAACGA GCGGACGCCC
    TACATCATGA AAACCACCAA GCATTTCAAT GACATCAGTA ACTTGATTGC TTCAGAAATC
    ATCCGCAATG AAGACATCAA TGCAAGAGTG AGCGCCATCG AGAAGTGGGT GGCTGTGGCT
    GACATATGCC GCTGCTTACA CAACTATAAC GCTGTGCTGG AAATCACCTC GTCCATGAAC
    CGGAGCGCAA TATTCCGGCT GAAAAAGACG TGGCTCAAAG TCTCTAAGCA GACAAAAGCT
    TTGATTGATA AGCTCCAAAA GCTTGTATCA TCAGAGGGCA GATTTAAGAA TCTGAGAGAA
    GCTCTGAAAA ATTGTGATCC ACCCTGTGTC CCTTACCTGG GGATGTACCT CACCGACCTG
    GCCTTCATCG AGGAGGGGAC ACCCAATTAC ACGGAGGATG GCCTGGTCAA CTTCTCCAAG
    ATGAGAATGA TATCTCATAT TATCCGAGAG ATCCGCCAGT TTCAACAAAC TGCGTATAAA
    ATAGAACACC AAGTGAAGGT AAGCCGCCAG TGCTCCGACC CTTGGGCTCT CACACAGGTG
    CTCTGTCTGG ACCATCCTCT TCCTCCCTCA TCCCTTCACA TCTACCCACT CCCTTTCTCT
    CTCTGGCTCC CCTGGGCTCA AAGGCCAACT CCTCCAGGAA GCCCTCCCTC TCTCCTCCCC
    CCCTCCCCCC GCCACCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272723.1
    CDS695..4615
    Translation

    Target ORF information:

    RefSeq Version XM_022417015.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTGCGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ATTTCACGGT TAACTTCAGC
    CATGAGAACC AGAAAGCCCT GGAGCTGAGG ACAGAGGATG CCAAGGACTG TGACGAGTGG
    GTGGCAGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CAGAGCACGA AGCGCTGATG
    CAAAAGTATC TGCACCTCCT GCAGATCGTG GAGACAGAGA AGACAGTGGC CAAACAGCTG
    CGGCGGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATTGCATCA
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACGCAGACCG TCACCCCCAA CGATGAAGAC
    AGTGACATCA AGAAGATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGCTCGCCGC ACGCTGATAG CATGCGGAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTATGTGCA GCAGCTCCAC
    ATCCTGGTTA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTCAACAGCG AGACCATCAT GTTCCTGCAC
    CAGATCTTCT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACCCT GGTCCTGGCT
    GACCTGTTCG ACATCCTGCT GCCGATGCTC AACATCTACC AGGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GGGACTTCGA CAAGCTGCTG
    AAGCACTATG AGGCCAAGCC CGACTGCGAG GAACGGACCC TGGAGACCTT CCTCACCTAC
    CCCATGTTTC AGATCCCCAG GTACATCCTG ACGCTGCATG AGCTCCTGGC GCACACCCCT
    CACGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ATGACGAAGT GAGTGAGACA GAGAACATCC GGAAAAACCT CGCCATCGAG
    CGCATGATCA TTGAAGGCTG CGAGATCCTG CTGGACACCA GCCAGACTTT TGTGAGACAA
    GGCTCGCTCA TCCAGGTGCC CATGTCTGAG AAGGGCAAGA TCACCAGGGG ACGCCTGGGG
    TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTCTCCAA GCACCTGATT
    ATCTGCACCA GAGGCTCTGG TGGGAAACTC CACCTGACCA AGAACGGGGT CATCTCCCTC
    ATCGACTGCA CCTTGCTGGA GGAGCCGGAG AGCACAGAGG AAGACGCCAA AGGATCCAGC
    CAAGACATAG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAAGACTC CCCGCCTTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAATGGG CTCATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACTG TGCCACAGAT GATCAAATCC GACGCCTCCT TGTACTGCGA TGACGTAGAC
    ATTCGCTTCA GCAAGACCAT GAACTCGTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GGGCGGCTGC TGGAACGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACG
    TTCCTGCACT CGTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGCTCTG GCCAAGCCTC GAGGGGCCCG
    GTGGGTCACC GTGAGCCCTG GGCCCCTGCC AGATCACTAG AGCTCTTATT CGCCAGCGGC
    CAGAACAACA AGCTTCTGTA TGGTGACCCT CCCAAGTCCC CGCGCGCCAC GCGCAAGTTC
    TCCTCACCGC CACCCCTGTC CATCACCAAG ACGTCATCCC CCAGCCGCCG GCGGAAGCTG
    TCGCTGAACA TCCCCATCAT CACGGGCGGC AAGGCCCTGG ACCTGGCCGC CCTCAGCTGC
    AGCTCCAACG GCTACACCAG CCTGTACTCG GCCGTGTCCC CCTTCAGCAA GGCCACGCTG
    GACACCAGCA AGCTCTTCGT ATCCAGCAGC TTTGCCAACA AGATTCCAGA CGAAGGTGAA
    ACGGCCACTG AGAAGCCCGA AGAGCCCGTG GCTCTCAGCA AGCAGAGCTC AGAAGTCTCC
    GTGAGGGAAG AGTCCGATAA CGATCAAACC CAGAGTGACG ACGGGGACAC TGAGGCGTCC
    CCAACCAAAT CGCCAACAAC GCCAAAGTCA GTCAAAAGCA AAAATTCCTC AGAGTTCCCC
    CTCTTCTCCT ACAACAACGG GGTGGTCATG ACCTCGTGTC GGGAGCTGGA CAACACACGC
    AGCGCCCTGT CCGCGGCCTC TGCCTTTGCC ATAGCCACGG CGGGAGCCAA CGAGGGCACC
    CCGAACAAAG AGAAGTACCG GAGGATGTCC CTGGCCAGCA CAGGGTTTCC CCCTGACCAG
    AGGAACGGGG ACAAGGAGTT CGTGATCCGC AGAGCGGCCA CCAACCGCGT CCTGAACGTG
    CTCCGCCACT GGGTCTCCAA ACACTCGCAG GACTTTGAGA CCAATGACGA GCTGCGATGC
    AAGGTGATCA GCTTCCTGGA GGAGGTCATG CACGACCCAG AGCTGCTGAC CCAGGAGAGG
    AAGGCTGCGG CCAACATTAT CAGGACTCTG ACCCAGGAGG ACCCAGGGGA CAACCAGATC
    ACACTGGAGG AGATCACGCA GATGGCCGAA GGCGTGAAGG CAGAGCCCTT TGAAAACCAC
    TCGGCCCTGG AGATAGCGGA GCAGCTGACC CTGCTGGACC ACCTGGTCTT CAAGAAGATC
    CCTTACGAGG AGTTCTTCGG GCAAGGCTGG ATGAAAGTGG AGAAGAACGA GCGGACGCCC
    TACATCATGA AAACCACCAA GCATTTCAAT GACATCAGTA ACTTGATTGC TTCAGAAATC
    ATCCGCAATG AAGACATCAA TGCAAGAGTG AGCGCCATCG AGAAGTGGGT GGCTGTGGCT
    GACATATGCC GCTGCTTACA CAACTATAAC GCTGTGCTGG AAATCACCTC GTCCATGAAC
    CGGAGCGCAA TATTCCGGCT GAAAAAGACG TGGCTCAAAG TCTCTAAGCA GACAAAAGCT
    TTGATTGATA AGCTCCAAAA GCTTGTATCA TCAGAGGGCA GATTTAAGAA TCTGAGAGAA
    GCTCTGAAAA ATTGTGATCC ACCCTGTGTC CCTTACCTGG GGATGTACCT CACCGACCTG
    GCCTTCATCG AGGAGGGGAC ACCCAATTAC ACGGAGGATG GCCTGGTCAA CTTCTCCAAG
    ATGAGAATGA TATCTCATAT TATCCGAGAG ATCCGCCAGT TTCAACAAAC TGCGTATAAA
    ATAGAACACC AAGTGAAGGT AAGCCGCCAG TGCTCCGACC CTTGGGCTCT CACACAGGTG
    CTCTGTCTGG ACCATCCTCT TCCTCCCTCA TCCCTTCACA TCTACCCACT CCCTTTCTCT
    CTCTGGCTCC CCTGGGCTCA AAGGCCAACT CCTCCAGGAA GCCCTCCCTC TCTCCTCCCC
    CCCTCCCCCC GCCACCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287267
    Clone ID Related Accession (Same CDS sequence) XM_022417016.1
    Accession Version XM_022417016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTGCGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ATTTCACGGT TAACTTCAGC
    CATGAGAACC AGAAAGCCCT GGAGCTGAGG ACAGAGGATG CCAAGGACTG TGACGAGTGG
    GTGGCAGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CAGAGCACGA AGCGCTGATG
    CAAAAGTATC TGCACCTCCT GCAGATCGTG GAGACAGAGA AGACAGTGGC CAAACAGCTG
    CGGCGGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATTGCATCA
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACGCAGACCG TCACCCCCAA CGATGAAGAC
    AGTGACATCA AGAAGATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGCTCGCCGC ACGCTGATAG CATGCGGAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTATGTGCA GCAGCTCCAC
    ATCCTGGTTA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTCAACAGCG AGACCATCAT GTTCCTGCAC
    CAGATCTTCT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACCCT GGTCCTGGCT
    GACCTGTTCG ACATCCTGCT GCCGATGCTC AACATCTACC AGGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GGGACTTCGA CAAGCTGCTG
    AAGCACTATG AGGCCAAGCC CGACTGCGAG GAACGGACCC TGGAGACCTT CCTCACCTAC
    CCCATGTTTC AGATCCCCAG GTACATCCTG ACGCTGCATG AGCTCCTGGC GCACACCCCT
    CACGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ATGACGAAGT GAGTGAGACA GAGAACATCC GGAAAAACCT CGCCATCGAG
    CGCATGATCA TTGAAGGCTG CGAGATCCTG CTGGACACCA GCCAGACTTT TGTGAGACAA
    GGCTCGCTCA TCCAGGTGCC CATGTCTGAG AAGGGCAAGA TCACCAGGGG ACGCCTGGGG
    TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTCTCCAA GCACCTGATT
    ATCTGCACCA GAGGCTCTGG TGGGAAACTC CACCTGACCA AGAACGGGGT CATCTCCCTC
    ATCGACTGCA CCTTGCTGGA GGAGCCGGAG AGCACAGAGG AAGACGCCAA AGGATCCAGC
    CAAGACATAG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAAGACTC CCCGCCTTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAATGGG CTCATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACTG TGCCACAGAT GATCAAATCC GACGCCTCCT TGTACTGCGA TGACGTAGAC
    ATTCGCTTCA GCAAGACCAT GAACTCGTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GGGCGGCTGC TGGAACGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACG
    TTCCTGCACT CGTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGATCAC TAGAGCTCTT ATTCGCCAGC
    GGCCAGAACA ACAAGCTTCT GTATGGTGAC CCTCCCAAGT CCCCGCGCGC CACGCGCAAG
    TTCTCCTCAC CGCCACCCCT GTCCATCACC AAGACGTCAT CCCCCAGCCG CCGGCGGAAG
    CTGTCGCTGA ACATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCCTGTAC TCGGCCGTGT CCCCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTT CGTATCCAGC AGCTTTGCCA ACAAGATTCC AGACGAAGGT
    GAAACGGCCA CTGAGAAGCC CGAAGAGCCC GTGGCTCTCA GCAAGCAGAG CTCAGAAGTC
    TCCGTGAGGG AAGAGTCCGA TAACGATCAA ACCCAGAGTG ACGACGGGGA CACTGAGGCG
    TCCCCAACCA AATCGCCAAC AACGCCAAAG TCAGTCAAAA GCAAAAATTC CTCAGAGTTC
    CCCCTCTTCT CCTACAACAA CGGGGTGGTC ATGACCTCGT GTCGGGAGCT GGACAACACA
    CGCAGCGCCC TGTCCGCGGC CTCTGCCTTT GCCATAGCCA CGGCGGGAGC CAACGAGGGC
    ACCCCGAACA AAGAGAAGTA CCGGAGGATG TCCCTGGCCA GCACAGGGTT TCCCCCTGAC
    CAGAGGAACG GGGACAAGGA GTTCGTGATC CGCAGAGCGG CCACCAACCG CGTCCTGAAC
    GTGCTCCGCC ACTGGGTCTC CAAACACTCG CAGGACTTTG AGACCAATGA CGAGCTGCGA
    TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTGCT GACCCAGGAG
    AGGAAGGCTG CGGCCAACAT TATCAGGACT CTGACCCAGG AGGACCCAGG GGACAACCAG
    ATCACACTGG AGGAGATCAC GCAGATGGCC GAAGGCGTGA AGGCAGAGCC CTTTGAAAAC
    CACTCGGCCC TGGAGATAGC GGAGCAGCTG ACCCTGCTGG ACCACCTGGT CTTCAAGAAG
    ATCCCTTACG AGGAGTTCTT CGGGCAAGGC TGGATGAAAG TGGAGAAGAA CGAGCGGACG
    CCCTACATCA TGAAAACCAC CAAGCATTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAAGACAT CAATGCAAGA GTGAGCGCCA TCGAGAAGTG GGTGGCTGTG
    GCTGACATAT GCCGCTGCTT ACACAACTAT AACGCTGTGC TGGAAATCAC CTCGTCCATG
    AACCGGAGCG CAATATTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACAAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTA TCATCAGAGG GCAGATTTAA GAATCTGAGA
    GAAGCTCTGA AAAATTGTGA TCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC
    CTGGCCTTCA TCGAGGAGGG GACACCCAAT TACACGGAGG ATGGCCTGGT CAACTTCTCC
    AAGATGAGAA TGATATCTCA TATTATCCGA GAGATCCGCC AGTTTCAACA AACTGCGTAT
    AAAATAGAAC ACCAAGTGAA GGTAAGCCGC CAGTGCTCCG ACCCTTGGGC TCTCACACAG
    GTGCTCTGTC TGGACCATCC TCTTCCTCCC TCATCCCTTC ACATCTACCC ACTCCCTTTC
    TCTCTCTGGC TCCCCTGGGC TCAAAGGCCA ACTCCTCCAG GAAGCCCTCC CTCTCTCCTC
    CCCCCCTCCC CCCGCCACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272724.1
    CDS1..3864
    Translation

    Target ORF information:

    RefSeq Version XM_022417016.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTGCGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ATTTCACGGT TAACTTCAGC
    CATGAGAACC AGAAAGCCCT GGAGCTGAGG ACAGAGGATG CCAAGGACTG TGACGAGTGG
    GTGGCAGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CAGAGCACGA AGCGCTGATG
    CAAAAGTATC TGCACCTCCT GCAGATCGTG GAGACAGAGA AGACAGTGGC CAAACAGCTG
    CGGCGGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATTGCATCA
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACGCAGACCG TCACCCCCAA CGATGAAGAC
    AGTGACATCA AGAAGATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGCTCGCCGC ACGCTGATAG CATGCGGAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTATGTGCA GCAGCTCCAC
    ATCCTGGTTA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTCAACAGCG AGACCATCAT GTTCCTGCAC
    CAGATCTTCT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACCCT GGTCCTGGCT
    GACCTGTTCG ACATCCTGCT GCCGATGCTC AACATCTACC AGGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GGGACTTCGA CAAGCTGCTG
    AAGCACTATG AGGCCAAGCC CGACTGCGAG GAACGGACCC TGGAGACCTT CCTCACCTAC
    CCCATGTTTC AGATCCCCAG GTACATCCTG ACGCTGCATG AGCTCCTGGC GCACACCCCT
    CACGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ATGACGAAGT GAGTGAGACA GAGAACATCC GGAAAAACCT CGCCATCGAG
    CGCATGATCA TTGAAGGCTG CGAGATCCTG CTGGACACCA GCCAGACTTT TGTGAGACAA
    GGCTCGCTCA TCCAGGTGCC CATGTCTGAG AAGGGCAAGA TCACCAGGGG ACGCCTGGGG
    TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTCTCCAA GCACCTGATT
    ATCTGCACCA GAGGCTCTGG TGGGAAACTC CACCTGACCA AGAACGGGGT CATCTCCCTC
    ATCGACTGCA CCTTGCTGGA GGAGCCGGAG AGCACAGAGG AAGACGCCAA AGGATCCAGC
    CAAGACATAG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAAGACTC CCCGCCTTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAATGGG CTCATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACTG TGCCACAGAT GATCAAATCC GACGCCTCCT TGTACTGCGA TGACGTAGAC
    ATTCGCTTCA GCAAGACCAT GAACTCGTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GGGCGGCTGC TGGAACGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACG
    TTCCTGCACT CGTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGATCAC TAGAGCTCTT ATTCGCCAGC
    GGCCAGAACA ACAAGCTTCT GTATGGTGAC CCTCCCAAGT CCCCGCGCGC CACGCGCAAG
    TTCTCCTCAC CGCCACCCCT GTCCATCACC AAGACGTCAT CCCCCAGCCG CCGGCGGAAG
    CTGTCGCTGA ACATCCCCAT CATCACGGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAGCTCCA ACGGCTACAC CAGCCTGTAC TCGGCCGTGT CCCCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTT CGTATCCAGC AGCTTTGCCA ACAAGATTCC AGACGAAGGT
    GAAACGGCCA CTGAGAAGCC CGAAGAGCCC GTGGCTCTCA GCAAGCAGAG CTCAGAAGTC
    TCCGTGAGGG AAGAGTCCGA TAACGATCAA ACCCAGAGTG ACGACGGGGA CACTGAGGCG
    TCCCCAACCA AATCGCCAAC AACGCCAAAG TCAGTCAAAA GCAAAAATTC CTCAGAGTTC
    CCCCTCTTCT CCTACAACAA CGGGGTGGTC ATGACCTCGT GTCGGGAGCT GGACAACACA
    CGCAGCGCCC TGTCCGCGGC CTCTGCCTTT GCCATAGCCA CGGCGGGAGC CAACGAGGGC
    ACCCCGAACA AAGAGAAGTA CCGGAGGATG TCCCTGGCCA GCACAGGGTT TCCCCCTGAC
    CAGAGGAACG GGGACAAGGA GTTCGTGATC CGCAGAGCGG CCACCAACCG CGTCCTGAAC
    GTGCTCCGCC ACTGGGTCTC CAAACACTCG CAGGACTTTG AGACCAATGA CGAGCTGCGA
    TGCAAGGTGA TCAGCTTCCT GGAGGAGGTC ATGCACGACC CAGAGCTGCT GACCCAGGAG
    AGGAAGGCTG CGGCCAACAT TATCAGGACT CTGACCCAGG AGGACCCAGG GGACAACCAG
    ATCACACTGG AGGAGATCAC GCAGATGGCC GAAGGCGTGA AGGCAGAGCC CTTTGAAAAC
    CACTCGGCCC TGGAGATAGC GGAGCAGCTG ACCCTGCTGG ACCACCTGGT CTTCAAGAAG
    ATCCCTTACG AGGAGTTCTT CGGGCAAGGC TGGATGAAAG TGGAGAAGAA CGAGCGGACG
    CCCTACATCA TGAAAACCAC CAAGCATTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAAGACAT CAATGCAAGA GTGAGCGCCA TCGAGAAGTG GGTGGCTGTG
    GCTGACATAT GCCGCTGCTT ACACAACTAT AACGCTGTGC TGGAAATCAC CTCGTCCATG
    AACCGGAGCG CAATATTCCG GCTGAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACAAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTA TCATCAGAGG GCAGATTTAA GAATCTGAGA
    GAAGCTCTGA AAAATTGTGA TCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC
    CTGGCCTTCA TCGAGGAGGG GACACCCAAT TACACGGAGG ATGGCCTGGT CAACTTCTCC
    AAGATGAGAA TGATATCTCA TATTATCCGA GAGATCCGCC AGTTTCAACA AACTGCGTAT
    AAAATAGAAC ACCAAGTGAA GGTAAGCCGC CAGTGCTCCG ACCCTTGGGC TCTCACACAG
    GTGCTCTGTC TGGACCATCC TCTTCCTCCC TCATCCCTTC ACATCTACCC ACTCCCTTTC
    TCTCTCTGGC TCCCCTGGGC TCAAAGGCCA ACTCCTCCAG GAAGCCCTCC CTCTCTCCTC
    CCCCCCTCCC CCCGCCACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35657
    Clone ID Related Accession (Same CDS sequence) XM_014112339.1
    Accession Version XM_014112339.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ras-specific guanine nucleotide-releasing factor 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTGCGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ATTTCACGGT TAACTTCAGC
    CATGAGAACC AGAAAGCCCT GGAGCTGAGG ACAGAGGATG CCAAGGACTG TGACGAGTGG
    GTGGCAGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CAGAGCACGA AGCGCTGATG
    CAAAAGTATC TGCACCTCCT GCAGATCGTG GAGACAGAGA AGACAGTGGC CAAACAGCTG
    CGGCGGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATTGCATCA
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACGCAGACCG TCACCCCCAA CGATGAAGAC
    AGTGACATCA AGAAGATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGCTCGCCGC ACGCTGATAG CATGCGGAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTATGTGCA GCAGCTCCAC
    ATCCTGGTTA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTCAACAGCG AGACCATCAT GTTCCTGCAC
    CAGATCTTCT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACCCT GGTCCTGGCT
    GACCTGTTCG ACATCCTGCT GCCGATGCTC AACATCTACC AGGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GGGACTTCGA CAAGCTGCTG
    AAGCACTATG AGGCCAAGCC CGACTGCGAG GAACGGACCC TGGAGACCTT CCTCACCTAC
    CCCATGTTTC AGATCCCCAG GTACATCCTG ACGCTGCATG AGCTCCTGGC GCACACCCCT
    CACGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ATGACGAAGT GAGTGAGACA GAGAACATCC GGAAAAACCT CGCCATCGAG
    CGCATGATCA TTGAAGGCTG CGAGATCCTG CTGGACACCA GCCAGACTTT TGTGAGACAA
    GGCTCGCTCA TCCAGGTGCC CATGTCTGAG AAGGGCAAGA TCACCAGGGG ACGCCTGGGG
    TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTCTCCAA GCACCTGATT
    ATCTGCACCA GAGGCTCTGG TGGGAAACTC CACCTGACCA AGAACGGGGT CATCTCCCTC
    ATCGACTGCA CCTTGCTGGA GGAGCCGGAG AGCACAGAGG AAGACGCCAA AGGATCCAGC
    CAAGACATAG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAAGACTC CCCGCCTTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAATGGG CTCATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACTG TGCCACAGAT GATCAAATCC GACGCCTCCT TGTACTGCGA TGACGTAGAC
    ATTCGCTTCA GCAAGACCAT GAACTCGTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GGGCGGCTGC TGGAACGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACG
    TTCCTGCACT CGTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGCTCTG GCCAAGCCTC GAGGGGCCCG
    GTGGGTCACC GTGAGCCCTG GGCCCCTGCC AGATCACTAG AGCTCTTATT CGCCAGCGGC
    CAGAACAACA AGCTTCTGTA TGGTGACCCT CCCAAGTCCC CGCGCGCCAC GCGCAAGTTC
    TCCTCACCGC CACCCCTGTC CATCACCAAG ACGTCATCCC CCAGCCGCCG GCGGAAGCTG
    TCGCTGAACA TCCCCATCAT CACGGGCGGC AAGGCCCTGG ACCTGGCCGC CCTCAGCTGC
    AGCTCCAACG GCTACACCAG CCTGTACTCG GCCGTGTCCC CCTTCAGCAA GGCCACGCTG
    GACACCAGCA AGCTCTTCGT ATCCAGCAGC TTTGCCAACA AGATTCCAGA CGAAGGTGAA
    ACGGCCACTG AGAAGCCCGA AGAGCCCGTG GCTCTCAGCA AGCAGAGCTC AGAAGTCTCC
    GTGAGGGAAG AGTCCGATAA CGATCAAACC CAGAGTGACG ACGGGGACAC TGAGGCGTCC
    CCAACCAAAT CGCCAACAAC GCCAAAGTCA GTCAAAAGCA AAAATTCCTC AGAGTTCCCC
    CTCTTCTCCT ACAACAACGG GGTGGTCATG ACCTCGTGTC GGGAGCTGGA CAACACACGC
    AGCGCCCTGT CCGCGGCCTC TGCCTTTGCC ATAGCCACGG CGGGAGCCAA CGAGGGCACC
    CCGAACAAAG AGAAGTACCG GAGGATGTCC CTGGCCAGCA CAGGGTTTCC CCCTGACCAG
    AGGAACGGGG ACAAGGAGTT CGTGATCCGC AGAGCGGCCA CCAACCGCGT CCTGAACGTG
    CTCCGCCACT GGGTCTCCAA ACACTCGCAG GACTTTGAGA CCAATGACGA GCTGCGATGC
    AAGGTGATCA GCTTCCTGGA GGAGGTCATG CACGACCCAG AGCTGCTGAC CCAGGAGAGG
    AAGGCTGCGG CCAACATTAT CAGGACTCTG ACCCAGGAGG ACCCAGGGGA CAACCAGATC
    ACACTGGAGG AGATCACGCA GATGGCCGAA GGCGTGAAGG CAGAGCCCTT TGAAAACCAC
    TCGGCCCTGG AGATAGCGGA GCAGCTGACC CTGCTGGACC ACCTGGTCTT CAAGAAGATC
    CCTTACGAGG AGTTCTTCGG GCAAGGCTGG ATGAAAGTGG AGAAGAACGA GCGGACGCCC
    TACATCATGA AAACCACCAA GCATTTCAAT GACATCAGTA ACTTGATTGC TTCAGAAATC
    ATCCGCAATG AAGACATCAA TGCAAGAGTG AGCGCCATCG AGAAGTGGGT GGCTGTGGCT
    GACATATGCC GCTGCTTACA CAACTATAAC GCTGTGCTGG AAATCACCTC GTCCATGAAC
    CGGAGCGCAA TATTCCGGCT GAAAAAGACG TGGCTCAAAG TCTCTAAGCA GACAAAAGCT
    TTGATTGATA AGCTCCAAAA GCTTGTATCA TCAGAGGGCA GATTTAAGAA TCTGAGAGAA
    GCTCTGAAAA ATTGTGATCC ACCCTGTGTC CCTTACCTGG GGATGTACCT CACCGACCTG
    GCCTTCATCG AGGAGGGGAC ACCCAATTAC ACGGAGGATG GCCTGGTCAA CTTCTCCAAG
    ATGAGAATGA TATCTCATAT TATCCGAGAG ATCCGCCAGT TTCAACAAAC TGCGTATAAA
    ATAGAACACC AAGTGAAGGT AAGCCGCCAG TGCTCCGACC CTTGGGCTCT CACACAGGTG
    CTCTGTCTGG ACCATCCTCT TCCTCCCTCA TCCCTTCACA TCTACCCACT CCCTTTCTCT
    CTCTGTGAGT TCTCCCAGCT TCAGGCAGCA GGTGTGAACC TTGTCACCTG CCCTTGGATG
    GTGGAGGATT ACATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967814.1
    CDS538..4455
    Translation

    Target ORF information:

    RefSeq Version XM_014112339.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112339.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGAAGG GGATCCGGCT GAACGATGGC CACGTCGCGT CCCTGGGGCT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTCTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCTGCGCTCT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ATTTCACGGT TAACTTCAGC
    CATGAGAACC AGAAAGCCCT GGAGCTGAGG ACAGAGGATG CCAAGGACTG TGACGAGTGG
    GTGGCAGCCA TCGCTCATGC CAGCTACCGG ACCCTGGCCA CAGAGCACGA AGCGCTGATG
    CAAAAGTATC TGCACCTCCT GCAGATCGTG GAGACAGAGA AGACAGTGGC CAAACAGCTG
    CGGCGGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATTGCATCA
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACGCAGACCG TCACCCCCAA CGATGAAGAC
    AGTGACATCA AGAAGATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGC
    AAGTGGAAGA CCATCATCCA GGACTACATC CGCTCGCCGC ACGCTGATAG CATGCGGAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCCGAGGCCG AGTATGTGCA GCAGCTCCAC
    ATCCTGGTTA ACAACTTCCT GCGCCCACTG CGGATGGCCG CCAGCTCCAA GAAGCCCCCC
    ATCACGCACG ACGACGTCAG CAGCATCTTC CTCAACAGCG AGACCATCAT GTTCCTGCAC
    CAGATCTTCT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACCCT GGTCCTGGCT
    GACCTGTTCG ACATCCTGCT GCCGATGCTC AACATCTACC AGGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCGCACTGC AAGCAGAACC GGGACTTCGA CAAGCTGCTG
    AAGCACTATG AGGCCAAGCC CGACTGCGAG GAACGGACCC TGGAGACCTT CCTCACCTAC
    CCCATGTTTC AGATCCCCAG GTACATCCTG ACGCTGCATG AGCTCCTGGC GCACACCCCT
    CACGAGCACG TGGAGCGCAA CAGCCTGGAC TATGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGGATAATGC ATGACGAAGT GAGTGAGACA GAGAACATCC GGAAAAACCT CGCCATCGAG
    CGCATGATCA TTGAAGGCTG CGAGATCCTG CTGGACACCA GCCAGACTTT TGTGAGACAA
    GGCTCGCTCA TCCAGGTGCC CATGTCTGAG AAGGGCAAGA TCACCAGGGG ACGCCTGGGG
    TCTCTGTCCC TAAAGAAAGA GGGTGAGCGG CAGTGCTTCC TGTTCTCCAA GCACCTGATT
    ATCTGCACCA GAGGCTCTGG TGGGAAACTC CACCTGACCA AGAACGGGGT CATCTCCCTC
    ATCGACTGCA CCTTGCTGGA GGAGCCGGAG AGCACAGAGG AAGACGCCAA AGGATCCAGC
    CAAGACATAG ACCACTTGGA TTTTAAGATC GGGGTGGAGC CCAAAGACTC CCCGCCTTTC
    ACGGTCATCC TCGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG GTGCAATGGG CTCATGATGA ACGCCTTTGA GGAGAACTCC
    AAGGTCACTG TGCCACAGAT GATCAAATCC GACGCCTCCT TGTACTGCGA TGACGTAGAC
    ATTCGCTTCA GCAAGACCAT GAACTCGTGC AAAGTGCTGC AGATCCGGTA CGCCAGCGTG
    GGGCGGCTGC TGGAACGGCT CACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACG
    TTCCTGCACT CGTACCGCGT CTTCACCACG GCCGTCGTGG TCCTGGACAA GCTCATCACC
    ATCTACAGGA AGCCCATCAG CGCCATCCCC GCCAGCTCTG GCCAAGCCTC GAGGGGCCCG
    GTGGGTCACC GTGAGCCCTG GGCCCCTGCC AGATCACTAG AGCTCTTATT CGCCAGCGGC
    CAGAACAACA AGCTTCTGTA TGGTGACCCT CCCAAGTCCC CGCGCGCCAC GCGCAAGTTC
    TCCTCACCGC CACCCCTGTC CATCACCAAG ACGTCATCCC CCAGCCGCCG GCGGAAGCTG
    TCGCTGAACA TCCCCATCAT CACGGGCGGC AAGGCCCTGG ACCTGGCCGC CCTCAGCTGC
    AGCTCCAACG GCTACACCAG CCTGTACTCG GCCGTGTCCC CCTTCAGCAA GGCCACGCTG
    GACACCAGCA AGCTCTTCGT ATCCAGCAGC TTTGCCAACA AGATTCCAGA CGAAGGTGAA
    ACGGCCACTG AGAAGCCCGA AGAGCCCGTG GCTCTCAGCA AGCAGAGCTC AGAAGTCTCC
    GTGAGGGAAG AGTCCGATAA CGATCAAACC CAGAGTGACG ACGGGGACAC TGAGGCGTCC
    CCAACCAAAT CGCCAACAAC GCCAAAGTCA GTCAAAAGCA AAAATTCCTC AGAGTTCCCC
    CTCTTCTCCT ACAACAACGG GGTGGTCATG ACCTCGTGTC GGGAGCTGGA CAACACACGC
    AGCGCCCTGT CCGCGGCCTC TGCCTTTGCC ATAGCCACGG CGGGAGCCAA CGAGGGCACC
    CCGAACAAAG AGAAGTACCG GAGGATGTCC CTGGCCAGCA CAGGGTTTCC CCCTGACCAG
    AGGAACGGGG ACAAGGAGTT CGTGATCCGC AGAGCGGCCA CCAACCGCGT CCTGAACGTG
    CTCCGCCACT GGGTCTCCAA ACACTCGCAG GACTTTGAGA CCAATGACGA GCTGCGATGC
    AAGGTGATCA GCTTCCTGGA GGAGGTCATG CACGACCCAG AGCTGCTGAC CCAGGAGAGG
    AAGGCTGCGG CCAACATTAT CAGGACTCTG ACCCAGGAGG ACCCAGGGGA CAACCAGATC
    ACACTGGAGG AGATCACGCA GATGGCCGAA GGCGTGAAGG CAGAGCCCTT TGAAAACCAC
    TCGGCCCTGG AGATAGCGGA GCAGCTGACC CTGCTGGACC ACCTGGTCTT CAAGAAGATC
    CCTTACGAGG AGTTCTTCGG GCAAGGCTGG ATGAAAGTGG AGAAGAACGA GCGGACGCCC
    TACATCATGA AAACCACCAA GCATTTCAAT GACATCAGTA ACTTGATTGC TTCAGAAATC
    ATCCGCAATG AAGACATCAA TGCAAGAGTG AGCGCCATCG AGAAGTGGGT GGCTGTGGCT
    GACATATGCC GCTGCTTACA CAACTATAAC GCTGTGCTGG AAATCACCTC GTCCATGAAC
    CGGAGCGCAA TATTCCGGCT GAAAAAGACG TGGCTCAAAG TCTCTAAGCA GACAAAAGCT
    TTGATTGATA AGCTCCAAAA GCTTGTATCA TCAGAGGGCA GATTTAAGAA TCTGAGAGAA
    GCTCTGAAAA ATTGTGATCC ACCCTGTGTC CCTTACCTGG GGATGTACCT CACCGACCTG
    GCCTTCATCG AGGAGGGGAC ACCCAATTAC ACGGAGGATG GCCTGGTCAA CTTCTCCAAG
    ATGAGAATGA TATCTCATAT TATCCGAGAG ATCCGCCAGT TTCAACAAAC TGCGTATAAA
    ATAGAACACC AAGTGAAGGT AAGCCGCCAG TGCTCCGACC CTTGGGCTCT CACACAGGTG
    CTCTGTCTGG ACCATCCTCT TCCTCCCTCA TCCCTTCACA TCTACCCACT CCCTTTCTCT
    CTCTGTGAGT TCTCCCAGCT TCAGGCAGCA GGTGTGAACC TTGTCACCTG CCCTTGGATG
    GTGGAGGATT ACATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.