MRPS9 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPS9
Entrez Gene ID	519302
Full Name	mitochondrial ribosomal protein S9
General protein information	Preferred Names mitochondrial ribosomal protein S9 Names 28S ribosomal protein S9, mitochondrial MRP-S9 S9mt
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001035332.1	NP_001030409.1	28S ribosomal protein S9, mitochondrial
XM_005212443.3	XP_005212500.1	28S ribosomal protein S9, mitochondrial isoform X1
XM_005212443.4	XP_005212500.1	28S ribosomal protein S9, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MRPS9	NP_872578.1
Pan troglodytes (chimpanzee)	MRPS9	XP_515671.1
Canis lupus familiaris (dog)	MRPS9	XP_531774.2
Rattus norvegicus (Norway rat)	Mrps9	NP_001094019.1
Danio rerio (zebrafish)	mrps9	NP_001038861.1
Bos taurus (cattle)	MRPS9	NP_001030409.1
Caenorhabditis elegans (roundworm)	mrps-9	NP_498815.1
	MRPS9	XP_001108643.1
Mus musculus (house mouse)	Mrps9	NP_076003.3
Drosophila melanogaster (fruit fly)	mRpS9	NP_524270.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010268	XP_319459.4
Xenopus tropicalis (tropical clawed frog)	mrps9	XP_002941200.1
Gallus gallus (chicken)	MRPS9	NP_001264686.1

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM structure of the small subunit of the mammalian mitochondrial ribosome.
Kaushal PS, Sharma MR, Booth TM, Haque EM, Tung CS, Sanbonmatsu KY, Spremulli LL, Agrawal RK
Proceedings of the National Academy of Sciences of the United States of America111(20)7284-9(2014 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

Identification of four proteins from the small subunit of the mammalian mitochondrial ribosome using a proteomics approach.
Koc EC, Burkhart W, Blackburn K, Koc H, Moseley A, Spremulli LL
Protein science : a publication of the Protein Society10(3)471-81(2001 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MRPS9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPS9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42659	NM_001035332.1 Latest version!	Bos taurus mitochondrial ribosomal protein S9 (MRPS9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa23780	XM_005212443.4 Latest version!	Bos taurus mitochondrial ribosomal protein S9 (MRPS9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OBa23780	XM_005212443.3	Bos taurus mitochondrial ribosomal protein S9 (MRPS9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPS9 ( NM_001035332.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001030409.1 Bos taurus mitochondrial ribosomal protein S9 (MRPS9), mRNA.

Price & Availability↑

CloneID	OBa42659
Clone ID Related Accession (Same CDS sequence)	NM_001035332.1
Accession Version	NM_001035332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	28S ribosomal protein S9, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT021542.1. On Nov 1, 2005 this sequence version replaced XM_597518.2. ##Evidence-Data-START## Transcript exon combination :: BT021542.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGCTC CCAGTGTGTC CTGCGGCGCA GCAGTTCCCT ACCGGCTCTT TCTTGCGGGT 
AGGGTTAGCT TCGCCCGGGA ACAAGGCCTC TGGAAAGCCG CGGCTTCTGG GCTGCAGACA 
GGTACCAGAT GCCAGATATT AAGGCTCAAA CATTCCCCGG CTGTAACAAC AACAAAAAAT 
GTTGCAGCCT TAAGACGTGA GAGTTACACG GTGGACTTTA TTAAAAAGCA GATTGAAGAG 
TTCAACATCG GGAAGAGACA CTTGGCCAAC ATGATGGGGG AGGACCCAGA AACCTTTACC 
CAGGAGGATG TTGATAGGGC GATCACTTAC CTTTTTCCCA GTGGTTTGTT TGAGAAGCGA 
GCCCGGCCCA TAATGAAGCA TCCCGAAGAG ATTTTTCCAA AACAGAGAGC TGTCCAGTGG 
GGAGAAGATG GCCGCCCATT TCATTTTCTC TTTTACACTG GCAAACAGTC ATACTATTCA 
TTAATGCATG AAGCGTATGG AAAGGTGCTC CACGCAGAAG AACGGCAGGA CCAGCTGCGA 
GCCAAGGGTC TGTTTTCAGA AAAATCGAAA TCCAAAGACC TGATTGGCAG CAGATGGCTG 
ATTAAGGAGG AACTGGAAGA AATGTTAGTA GAAAAACTGT CAGATCAAGA CTATGCACAG 
TTCATCCGGC TGCTGGAAAG GTTATCAGCC TTGCCGTGCG ATGCAGCTGA AGAGGAATTT 
GTGGGCAGGT TCCGCAGGAC AGTGACCGTT CAGTCGAAGA AACATCTGAT CGAGCCTTTG 
CAGTATGACG AACAGGGCAT GGCCTTCAGC ACAGGCCAAG GTAAGAGAAA GACTGCAAAT 
GCAGAGGCGG TTGTTTATGG CCATGGCAGT GGGAAGATAG AAATAAATGG GGTCGACTAC 
CTGCTCTACT TCCCAGTGAC CCAAGACAGA GAGCAGCTCA TGTTCCCCTT TCATTTCCTC 
GACCGGCTTG GGAAGCACGA CGTGACCTGC ACGGTGTCGG GGGGCGGGCG GTCGTCACAG 
GCGGGGGCCA TCCGCCTGGC CATGTCCAGA GCCCTGTGCA GCTTCATCAC CGAGGATGAG 
GTTGAATGGA TGCGCCAAGG TACGGCTAGT TTGAGTTTCT GTGAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030409.1
CDS	12..1142
Translation	MAAPSVSCGAAVPYRLFLAGRVSFAREQGLWKAAASGLQTGTRCQILRLKHSPAVTTTKNVAALRRESYTVDFIKKQIEEFNIGKRHLANMMGEDPETFTQEDVDRAITYLFPSGLFEKRARPIMKHPEEIFPKQRAVQWGEDGRPFHFLFYTGKQSYYSLMHEAYGKVLHAEERQDQLRAKGLFSEKSKSKDLIGSRWLIKEELEEMLVEKLSDQDYAQFIRLLERLSALPCDAAEEEFVGRFRRTVTVQSKKHLIEPLQYDEQGMAFSTGQGKRKTANAEAVVYGHGSGKIEINGVDYLLYFPVTQDREQLMFPFHFLDRLGKHDVTCTVSGGGRSSQAGAIRLAMSRALCSFITEDEVEWMRQGTASLSFCEP

Target ORF information:

RefSeq Version	NM_001035332.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mitochondrial ribosomal protein S9 (MRPS9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001035332.1

ORF Insert Sequence:

ATGGCAGCTC CCAGTGTGTC CTGCGGCGCA GCAGTTCCCT ACCGGCTCTT TCTTGCGGGT 
AGGGTTAGCT TCGCCCGGGA ACAAGGCCTC TGGAAAGCCG CGGCTTCTGG GCTGCAGACA 
GGTACCAGAT GCCAGATATT AAGGCTCAAA CATTCCCCGG CTGTAACAAC AACAAAAAAT 
GTTGCAGCCT TAAGACGTGA GAGTTACACG GTGGACTTTA TTAAAAAGCA GATTGAAGAG 
TTCAACATCG GGAAGAGACA CTTGGCCAAC ATGATGGGGG AGGACCCAGA AACCTTTACC 
CAGGAGGATG TTGATAGGGC GATCACTTAC CTTTTTCCCA GTGGTTTGTT TGAGAAGCGA 
GCCCGGCCCA TAATGAAGCA TCCCGAAGAG ATTTTTCCAA AACAGAGAGC TGTCCAGTGG 
GGAGAAGATG GCCGCCCATT TCATTTTCTC TTTTACACTG GCAAACAGTC ATACTATTCA 
TTAATGCATG AAGCGTATGG AAAGGTGCTC CACGCAGAAG AACGGCAGGA CCAGCTGCGA 
GCCAAGGGTC TGTTTTCAGA AAAATCGAAA TCCAAAGACC TGATTGGCAG CAGATGGCTG 
ATTAAGGAGG AACTGGAAGA AATGTTAGTA GAAAAACTGT CAGATCAAGA CTATGCACAG 
TTCATCCGGC TGCTGGAAAG GTTATCAGCC TTGCCGTGCG ATGCAGCTGA AGAGGAATTT 
GTGGGCAGGT TCCGCAGGAC AGTGACCGTT CAGTCGAAGA AACATCTGAT CGAGCCTTTG 
CAGTATGACG AACAGGGCAT GGCCTTCAGC ACAGGCCAAG GTAAGAGAAA GACTGCAAAT 
GCAGAGGCGG TTGTTTATGG CCATGGCAGT GGGAAGATAG AAATAAATGG GGTCGACTAC 
CTGCTCTACT TCCCAGTGAC CCAAGACAGA GAGCAGCTCA TGTTCCCCTT TCATTTCCTC 
GACCGGCTTG GGAAGCACGA CGTGACCTGC ACGGTGTCGG GGGGCGGGCG GTCGTCACAG 
GCGGGGGCCA TCCGCCTGGC CATGTCCAGA GCCCTGTGCA GCTTCATCAC CGAGGATGAG 
GTTGAATGGA TGCGCCAAGG TACGGCTAGT TTGAGTTTCT GTGAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS9 ( XM_005212443.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212500.1 Bos taurus mitochondrial ribosomal protein S9 (MRPS9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23780
Clone ID Related Accession (Same CDS sequence)	XM_005212443.3 , XM_005212443.4
Accession Version	XM_005212443.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	28S ribosomal protein S9, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104288.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTC CCAGTGTGTC CTGCGGCGCA GCAGTTCCCT ACCGGCTCTT TCTTGCGGGT 
AGGGTTAGCT TCGCCCGGGA ACAAGGCCTC TGGAAAGCCG CGGCTTCTGG GCTGCAGACA 
GGTACCAGAT GCCAGATATT AAGGCTCAAA CATTCCCCGG CTGTAACAAC AACAAAAAAT 
GTTGCAGCCT TAAGACGTGA GAGTTACACG GTGGACTTTA TTAAAAAGCA GATTGAAGAG 
TTCAACATCG GGAAGAGACA CTTGGCCAAC ATGATGGGGG AGGACCCAGA AACCTTTACC 
CAGGAGGATG TTGATAGGGC GATCACTTAC CTTTTTCCCA GTGGTTTGTT TGAGAAGCGA 
GCCCGGCCCA TAATGAAGCA TCCCGAAGAG ATTTTTCCAA AACAGAGAGC TGTCCAGTGG 
GGAGAAGATG GCCGCCCATT TCATTTTCTC TTTTACACTG GCAAACAGTC ATACTATTCA 
TTAATGCATG AAGCGTATGG AAAGGTGCTC CACGCAGAAG AACGGCAGGA CCAGCTGCGA 
GCCAAGGGTC TGTTTTCAGA AAAATCGAAA TCCAAAGACC TGATTGGCAG CAGATGGCTG 
ATTAAGGAGG AACTGGAAGA AATGTTAGTA GAAAAACTGT CAGATCAAGA CTATGCACAG 
TTCATCCGGC TGCTGGAAAG GTTATCAGCC TTGCCGTGCG ATGCAGCTGA AGAGGAATTT 
GTGGGCAGGT TCCGCAGGAC AGTGACCGTT CAGTCGAAGA AACATCTGAT CGAGCCTTTG 
CAGTATGACG AACAGGGCAT GGCCTTCAGC ACAGGCCAAG GTAAGAGAAA GACTGCAAAT 
GCAGAGGCGG TTGTTTATGG CCATGGCAGT GGGAAGATAG AAATAAATGG GGTCGACTAC 
CTGCTCTACT TCCCAGTGAC CCAAGACAGA GAGCAGCTCA TGTTCCCCTT TCATTTCCTC 
GACCGGCTTG GGAAGCACGA CGTGACCTGC ACGGTGTCGG GGGGCGGGCG GTCGTCACAG 
GCGGGGGCCA TCCGCCTGGC CATGTCCAGA GCCCTGTGCA GCTTCATCAC CGAGGATGAG 
GTTGAATGGA TGCGCCAAGC TGGACTGCTC ACCACGGATC CCCGTGTCCG AGAGCGTAAG 
AAGCCAGGCC AGGAGGGCGC CCGCAGGAAG TTCACCTGGA AGAAACGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212500.1
CDS	296..1486
Translation	MAAPSVSCGAAVPYRLFLAGRVSFAREQGLWKAAASGLQTGTRCQILRLKHSPAVTTTKNVAALRRESYTVDFIKKQIEEFNIGKRHLANMMGEDPETFTQEDVDRAITYLFPSGLFEKRARPIMKHPEEIFPKQRAVQWGEDGRPFHFLFYTGKQSYYSLMHEAYGKVLHAEERQDQLRAKGLFSEKSKSKDLIGSRWLIKEELEEMLVEKLSDQDYAQFIRLLERLSALPCDAAEEEFVGRFRRTVTVQSKKHLIEPLQYDEQGMAFSTGQGKRKTANAEAVVYGHGSGKIEINGVDYLLYFPVTQDREQLMFPFHFLDRLGKHDVTCTVSGGGRSSQAGAIRLAMSRALCSFITEDEVEWMRQAGLLTTDPRVRERKKPGQEGARRKFTWKKR

Target ORF information:

RefSeq Version	XM_005212443.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mitochondrial ribosomal protein S9 (MRPS9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212443.3

ORF Insert Sequence:

ATGGCAGCTC CCAGTGTGTC CTGCGGCGCA GCAGTTCCCT ACCGGCTCTT TCTTGCGGGT 
AGGGTTAGCT TCGCCCGGGA ACAAGGCCTC TGGAAAGCCG CGGCTTCTGG GCTGCAGACA 
GGTACCAGAT GCCAGATATT AAGGCTCAAA CATTCCCCGG CTGTAACAAC AACAAAAAAT 
GTTGCAGCCT TAAGACGTGA GAGTTACACG GTGGACTTTA TTAAAAAGCA GATTGAAGAG 
TTCAACATCG GGAAGAGACA CTTGGCCAAC ATGATGGGGG AGGACCCAGA AACCTTTACC 
CAGGAGGATG TTGATAGGGC GATCACTTAC CTTTTTCCCA GTGGTTTGTT TGAGAAGCGA 
GCCCGGCCCA TAATGAAGCA TCCCGAAGAG ATTTTTCCAA AACAGAGAGC TGTCCAGTGG 
GGAGAAGATG GCCGCCCATT TCATTTTCTC TTTTACACTG GCAAACAGTC ATACTATTCA 
TTAATGCATG AAGCGTATGG AAAGGTGCTC CACGCAGAAG AACGGCAGGA CCAGCTGCGA 
GCCAAGGGTC TGTTTTCAGA AAAATCGAAA TCCAAAGACC TGATTGGCAG CAGATGGCTG 
ATTAAGGAGG AACTGGAAGA AATGTTAGTA GAAAAACTGT CAGATCAAGA CTATGCACAG 
TTCATCCGGC TGCTGGAAAG GTTATCAGCC TTGCCGTGCG ATGCAGCTGA AGAGGAATTT 
GTGGGCAGGT TCCGCAGGAC AGTGACCGTT CAGTCGAAGA AACATCTGAT CGAGCCTTTG 
CAGTATGACG AACAGGGCAT GGCCTTCAGC ACAGGCCAAG GTAAGAGAAA GACTGCAAAT 
GCAGAGGCGG TTGTTTATGG CCATGGCAGT GGGAAGATAG AAATAAATGG GGTCGACTAC 
CTGCTCTACT TCCCAGTGAC CCAAGACAGA GAGCAGCTCA TGTTCCCCTT TCATTTCCTC 
GACCGGCTTG GGAAGCACGA CGTGACCTGC ACGGTGTCGG GGGGCGGGCG GTCGTCACAG 
GCGGGGGCCA TCCGCCTGGC CATGTCCAGA GCCCTGTGCA GCTTCATCAC CGAGGATGAG 
GTTGAATGGA TGCGCCAAGC TGGACTGCTC ACCACGGATC CCCGTGTCCG AGAGCGTAAG 
AAGCCAGGCC AGGAGGGCGC CCGCAGGAAG TTCACCTGGA AGAAACGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS9 ( XM_005212443.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212500.1 Bos taurus mitochondrial ribosomal protein S9 (MRPS9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23780
Clone ID Related Accession (Same CDS sequence)	XM_005212443.3 , XM_005212443.4
Accession Version	XM_005212443.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	28S ribosomal protein S9, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212443.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTC CCAGTGTGTC CTGCGGCGCA GCAGTTCCCT ACCGGCTCTT TCTTGCGGGT 
AGGGTTAGCT TCGCCCGGGA ACAAGGCCTC TGGAAAGCCG CGGCTTCTGG GCTGCAGACA 
GGTACCAGAT GCCAGATATT AAGGCTCAAA CATTCCCCGG CTGTAACAAC AACAAAAAAT 
GTTGCAGCCT TAAGACGTGA GAGTTACACG GTGGACTTTA TTAAAAAGCA GATTGAAGAG 
TTCAACATCG GGAAGAGACA CTTGGCCAAC ATGATGGGGG AGGACCCAGA AACCTTTACC 
CAGGAGGATG TTGATAGGGC GATCACTTAC CTTTTTCCCA GTGGTTTGTT TGAGAAGCGA 
GCCCGGCCCA TAATGAAGCA TCCCGAAGAG ATTTTTCCAA AACAGAGAGC TGTCCAGTGG 
GGAGAAGATG GCCGCCCATT TCATTTTCTC TTTTACACTG GCAAACAGTC ATACTATTCA 
TTAATGCATG AAGCGTATGG AAAGGTGCTC CACGCAGAAG AACGGCAGGA CCAGCTGCGA 
GCCAAGGGTC TGTTTTCAGA AAAATCGAAA TCCAAAGACC TGATTGGCAG CAGATGGCTG 
ATTAAGGAGG AACTGGAAGA AATGTTAGTA GAAAAACTGT CAGATCAAGA CTATGCACAG 
TTCATCCGGC TGCTGGAAAG GTTATCAGCC TTGCCGTGCG ATGCAGCTGA AGAGGAATTT 
GTGGGCAGGT TCCGCAGGAC AGTGACCGTT CAGTCGAAGA AACATCTGAT CGAGCCTTTG 
CAGTATGACG AACAGGGCAT GGCCTTCAGC ACAGGCCAAG GTAAGAGAAA GACTGCAAAT 
GCAGAGGCGG TTGTTTATGG CCATGGCAGT GGGAAGATAG AAATAAATGG GGTCGACTAC 
CTGCTCTACT TCCCAGTGAC CCAAGACAGA GAGCAGCTCA TGTTCCCCTT TCATTTCCTC 
GACCGGCTTG GGAAGCACGA CGTGACCTGC ACGGTGTCGG GGGGCGGGCG GTCGTCACAG 
GCGGGGGCCA TCCGCCTGGC CATGTCCAGA GCCCTGTGCA GCTTCATCAC CGAGGATGAG 
GTTGAATGGA TGCGCCAAGC TGGACTGCTC ACCACGGATC CCCGTGTCCG AGAGCGTAAG 
AAGCCAGGCC AGGAGGGCGC CCGCAGGAAG TTCACCTGGA AGAAACGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212500.1
CDS	359..1549
Translation	MAAPSVSCGAAVPYRLFLAGRVSFAREQGLWKAAASGLQTGTRCQILRLKHSPAVTTTKNVAALRRESYTVDFIKKQIEEFNIGKRHLANMMGEDPETFTQEDVDRAITYLFPSGLFEKRARPIMKHPEEIFPKQRAVQWGEDGRPFHFLFYTGKQSYYSLMHEAYGKVLHAEERQDQLRAKGLFSEKSKSKDLIGSRWLIKEELEEMLVEKLSDQDYAQFIRLLERLSALPCDAAEEEFVGRFRRTVTVQSKKHLIEPLQYDEQGMAFSTGQGKRKTANAEAVVYGHGSGKIEINGVDYLLYFPVTQDREQLMFPFHFLDRLGKHDVTCTVSGGGRSSQAGAIRLAMSRALCSFITEDEVEWMRQAGLLTTDPRVRERKKPGQEGARRKFTWKKR

Target ORF information:

RefSeq Version	XM_005212443.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mitochondrial ribosomal protein S9 (MRPS9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212443.4

ORF Insert Sequence:

ATGGCAGCTC CCAGTGTGTC CTGCGGCGCA GCAGTTCCCT ACCGGCTCTT TCTTGCGGGT 
AGGGTTAGCT TCGCCCGGGA ACAAGGCCTC TGGAAAGCCG CGGCTTCTGG GCTGCAGACA 
GGTACCAGAT GCCAGATATT AAGGCTCAAA CATTCCCCGG CTGTAACAAC AACAAAAAAT 
GTTGCAGCCT TAAGACGTGA GAGTTACACG GTGGACTTTA TTAAAAAGCA GATTGAAGAG 
TTCAACATCG GGAAGAGACA CTTGGCCAAC ATGATGGGGG AGGACCCAGA AACCTTTACC 
CAGGAGGATG TTGATAGGGC GATCACTTAC CTTTTTCCCA GTGGTTTGTT TGAGAAGCGA 
GCCCGGCCCA TAATGAAGCA TCCCGAAGAG ATTTTTCCAA AACAGAGAGC TGTCCAGTGG 
GGAGAAGATG GCCGCCCATT TCATTTTCTC TTTTACACTG GCAAACAGTC ATACTATTCA 
TTAATGCATG AAGCGTATGG AAAGGTGCTC CACGCAGAAG AACGGCAGGA CCAGCTGCGA 
GCCAAGGGTC TGTTTTCAGA AAAATCGAAA TCCAAAGACC TGATTGGCAG CAGATGGCTG 
ATTAAGGAGG AACTGGAAGA AATGTTAGTA GAAAAACTGT CAGATCAAGA CTATGCACAG 
TTCATCCGGC TGCTGGAAAG GTTATCAGCC TTGCCGTGCG ATGCAGCTGA AGAGGAATTT 
GTGGGCAGGT TCCGCAGGAC AGTGACCGTT CAGTCGAAGA AACATCTGAT CGAGCCTTTG 
CAGTATGACG AACAGGGCAT GGCCTTCAGC ACAGGCCAAG GTAAGAGAAA GACTGCAAAT 
GCAGAGGCGG TTGTTTATGG CCATGGCAGT GGGAAGATAG AAATAAATGG GGTCGACTAC 
CTGCTCTACT TCCCAGTGAC CCAAGACAGA GAGCAGCTCA TGTTCCCCTT TCATTTCCTC 
GACCGGCTTG GGAAGCACGA CGTGACCTGC ACGGTGTCGG GGGGCGGGCG GTCGTCACAG 
GCGGGGGCCA TCCGCCTGGC CATGTCCAGA GCCCTGTGCA GCTTCATCAC CGAGGATGAG 
GTTGAATGGA TGCGCCAAGC TGGACTGCTC ACCACGGATC CCCGTGTCCG AGAGCGTAAG 
AAGCCAGGCC AGGAGGGCGC CCGCAGGAAG TTCACCTGGA AGAAACGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM structure of the small subunit of the mammalian mitochondrial ribosome.
Proceedings of the National Academy of Sciences of the United States of America111(20)7284-9(2014 May)
Kaushal PS,Sharma MR,Booth TM,Haque EM,Tung CS,Sanbonmatsu KY,Spremulli LL,Agrawal RK

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

Identification of four proteins from the small subunit of the mammalian mitochondrial ribosome using a proteomics approach.
Protein science : a publication of the Protein Society10(3)471-81(2001 Mar)
Koc EC,Burkhart W,Blackburn K,Koc H,Moseley A,Spremulli LL

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MRPS9 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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