ANO4 cDNA ORF clone, Bos taurus(cattle)

The following ANO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa19434 NM_001102050.1
Latest version!
Bos taurus anoctamin 4 (ANO4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19431 XM_024991747.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19431 XM_005206590.4
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19431 XM_005206591.3
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19431 XM_015471090.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19431 XM_015471091.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19432 XM_024991748.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19432 XM_005206594.4
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19432 XM_024991753.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19432 XM_024991754.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19434 XM_005206596.4
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19434 XM_024991752.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa234388 XM_024991749.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa234389 XM_024991750.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa234390 XM_024991755.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa90751 XM_015471092.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa90752 XM_015471093.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa234381 XM_024991738.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa234381 XM_024991739.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa234382 XM_024991740.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa234383 XM_024991741.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa234384 XM_024991743.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa234385 XM_024991744.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa234386 XM_024991745.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa234387 XM_024991746.1
Latest version!
Bos taurus anoctamin 4 (ANO4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OBa19431 XM_005206590.3 Bos taurus anoctamin 4 (ANO4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19434 XM_005206596.3 Bos taurus anoctamin 4 (ANO4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19432 XM_005206594.3 Bos taurus anoctamin 4 (ANO4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa19434
    Clone ID Related Accession (Same CDS sequence) NM_001102050.1 , XM_024991752.1 , XM_005206596.4 , XM_005206596.3
    Accession Version NM_001102050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Bos taurus(cattle)
    Product anoctamin-4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC147937.1. On Aug 21, 2007 this sequence version replaced XM_596939.3. ##Evidence-Data-START## Transcript exon combination :: BC147937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145422, SAMN03145439 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGTGGCC 
    AAGGATGTCA ATATTCTTTT TGATGAATTA GAAGCTGTCA ACAGTCCTTG CAAAGATGAC
    GACTCTCTCC TTCACCCTGG AAATCTTACC AGTACTTCAG ATGATGCTAG CAGGTTGGAA
    GCCGGGGGAG AGACAGTACC AGAAAAAAAC AAATTAAATG GCCTTTACTT CAGAGACGGA
    AAATGTCGAA TTGACTACAT TCTTGTGTAC AGAAAATCCA ACCCCCAGAT GGAAAAGAGA
    GAAGTATTTG AAAGAAATAT TAGAGCAGAA GGATTACAAA TGGAGAAAGA GTCCTCTCTA
    ATAAATAGTG ACATTATCTT TGTGAAGTTG CATGCCCCAT GGGAAGTGCT TGGAAGATAT
    GCAGAACAAA TGAATGTAAG AATGCCTTTC AGGAGAAAAA TCTATTACCT GCCCCGCCGG
    TACAAGTTCA TGAGCAGGAT CGATAAACAA ATAAGCAGGT TTCGGAGATG GTTACCTAAG
    AAGCCAATGA GGCTGGACAA GGAGACACTG CCAGACCTGG AGGAGAATGA CTGCTACACT
    GCCCCTTTCA GCCAGCAAAG GATCCATCAC TTCATCATAC ACAACAAAGA CACTTTCTTC
    AACAATGCCA CAAGAAGTAG AATTGTACAT CATATTTTAC AAAGAATAAA GTATGAAGAA
    GGAAAAAACA AAATTGGTCT GAATCGTTTG CTTACTAATG GCTCCTATGA AGCTGCATTT
    CCTCTGCACG AGGGAAGTTA CAGAAGTAAA AACTCCATCA GAACCCACGG AGCGGTAAAC
    CACCGACATC TGCTCTATGA GTGCTGGGCG TCCTGGGGTG TGTGGTACAA ATACCAGCCT
    TTGGATCTTG TAAGGCGGTA CTTTGGAGAG AAGATTGGGT TGTACTTTGC CTGGTTGGGC
    TGGTACACCG GCATGCTCTT CCCAGCTGCC TTCATTGGAT TGTTTGTCTT TTTGTATGGC
    GTCATCACTC TGGATCACTG CCAAGTCAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095520.1
    CDS494..3256
    Translation

    Target ORF information:

    RefSeq Version NM_001102050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGTGGCC 
    AAGGATGTCA ATATTCTTTT TGATGAATTA GAAGCTGTCA ACAGTCCTTG CAAAGATGAC
    GACTCTCTCC TTCACCCTGG AAATCTTACC AGTACTTCAG ATGATGCTAG CAGGTTGGAA
    GCCGGGGGAG AGACAGTACC AGAAAAAAAC AAATTAAATG GCCTTTACTT CAGAGACGGA
    AAATGTCGAA TTGACTACAT TCTTGTGTAC AGAAAATCCA ACCCCCAGAT GGAAAAGAGA
    GAAGTATTTG AAAGAAATAT TAGAGCAGAA GGATTACAAA TGGAGAAAGA GTCCTCTCTA
    ATAAATAGTG ACATTATCTT TGTGAAGTTG CATGCCCCAT GGGAAGTGCT TGGAAGATAT
    GCAGAACAAA TGAATGTAAG AATGCCTTTC AGGAGAAAAA TCTATTACCT GCCCCGCCGG
    TACAAGTTCA TGAGCAGGAT CGATAAACAA ATAAGCAGGT TTCGGAGATG GTTACCTAAG
    AAGCCAATGA GGCTGGACAA GGAGACACTG CCAGACCTGG AGGAGAATGA CTGCTACACT
    GCCCCTTTCA GCCAGCAAAG GATCCATCAC TTCATCATAC ACAACAAAGA CACTTTCTTC
    AACAATGCCA CAAGAAGTAG AATTGTACAT CATATTTTAC AAAGAATAAA GTATGAAGAA
    GGAAAAAACA AAATTGGTCT GAATCGTTTG CTTACTAATG GCTCCTATGA AGCTGCATTT
    CCTCTGCACG AGGGAAGTTA CAGAAGTAAA AACTCCATCA GAACCCACGG AGCGGTAAAC
    CACCGACATC TGCTCTATGA GTGCTGGGCG TCCTGGGGTG TGTGGTACAA ATACCAGCCT
    TTGGATCTTG TAAGGCGGTA CTTTGGAGAG AAGATTGGGT TGTACTTTGC CTGGTTGGGC
    TGGTACACCG GCATGCTCTT CCCAGCTGCC TTCATTGGAT TGTTTGTCTT TTTGTATGGC
    GTCATCACTC TGGATCACTG CCAAGTCAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19431
    Clone ID Related Accession (Same CDS sequence) XM_015471090.1 , XM_005206590.3 , XM_005206591.3 , XM_005206590.4 , XM_015471091.1 , XM_024991747.1
    Accession Version XM_015471090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326576.1
    CDS445..3366
    Translation

    Target ORF information:

    RefSeq Version XM_015471090.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19431
    Clone ID Related Accession (Same CDS sequence) XM_015471090.1 , XM_005206590.3 , XM_005206591.3 , XM_005206590.4 , XM_015471091.1 , XM_024991747.1
    Accession Version XM_005206590.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206647.1
    CDS836..3757
    Translation

    Target ORF information:

    RefSeq Version XM_005206590.3
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206590.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19431
    Clone ID Related Accession (Same CDS sequence) XM_015471090.1 , XM_005206590.3 , XM_005206591.3 , XM_005206590.4 , XM_015471091.1 , XM_024991747.1
    Accession Version XM_005206591.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206648.1
    CDS371..3292
    Translation

    Target ORF information:

    RefSeq Version XM_005206591.3
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206591.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19431
    Clone ID Related Accession (Same CDS sequence) XM_015471090.1 , XM_005206590.3 , XM_005206591.3 , XM_005206590.4 , XM_015471091.1 , XM_024991747.1
    Accession Version XM_005206590.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206590.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206647.1
    CDS1130..4051
    Translation

    Target ORF information:

    RefSeq Version XM_005206590.4
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206590.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19431
    Clone ID Related Accession (Same CDS sequence) XM_015471090.1 , XM_005206590.3 , XM_005206591.3 , XM_005206590.4 , XM_015471091.1 , XM_024991747.1
    Accession Version XM_015471091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326577.1
    CDS372..3293
    Translation

    Target ORF information:

    RefSeq Version XM_015471091.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19431
    Clone ID Related Accession (Same CDS sequence) XM_015471090.1 , XM_005206590.3 , XM_005206591.3 , XM_005206590.4 , XM_015471091.1 , XM_024991747.1
    Accession Version XM_024991747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847515.1
    CDS90..3011
    Translation

    Target ORF information:

    RefSeq Version XM_024991747.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGAGAAAAAT CTATTACCTG CCCCGCCGGT ACAAGTTCAT GAGCAGGATC
    GATAAACAAA TAAGCAGGTT TCGGAGATGG TTACCTAAGA AGCCAATGAG GCTGGACAAG
    GAGACACTGC CAGACCTGGA GGAGAATGAC TGCTACACTG CCCCTTTCAG CCAGCAAAGG
    ATCCATCACT TCATCATACA CAACAAAGAC ACTTTCTTCA ACAATGCCAC AAGAAGTAGA
    ATTGTACATC ATATTTTACA AAGAATAAAG TATGAAGAAG GAAAAAACAA AATTGGTCTG
    AATCGTTTGC TTACTAATGG CTCCTATGAA GCTGCATTTC CTCTGCACGA GGGAAGTTAC
    AGAAGTAAAA ACTCCATCAG AACCCACGGA GCGGTAAACC ACCGACATCT GCTCTATGAG
    TGCTGGGCGT CCTGGGGTGT GTGGTACAAA TACCAGCCTT TGGATCTTGT AAGGCGGTAC
    TTTGGAGAGA AGATTGGGTT GTACTTTGCC TGGTTGGGCT GGTACACCGG CATGCTCTTC
    CCAGCTGCCT TCATTGGATT GTTTGTCTTT TTGTATGGCG TCATCACTCT GGATCACTGC
    CAAGTCAGTA AAGAAGTCTG CCAAGCTACA GATATCATCA TGTGTCCTGT GTGTGATAAA
    TACTGTCCAT TCATGAGGCT GTCAGACAGC TGCGTTTATG CCAAGGTAAC CCATCTTTTT
    GACAATGGAG CCACTGTTTT CTTTGCTGTT TTCATGGCAG TCTGGGCAAC AGTTTTCCTA
    GAGTTTTGGA AGAGACGGAG AGCAGTAATT GCTTATGACT GGGATTTGAT AGACTGGGAA
    GAAGAGGAGG AAGAAATACG GCCCCAGTTT GAAGCCAAGT ATTCCAAGAA AGAGAGGATG
    AATCCCATTT CCGGCAAGCC AGAACCATAT CAAGCATTTG CAGATAAATG CAGCCGACTT
    ATTGTTTCTG CATCTGGAAT ATTTTTTATG ATCTGCGTGG TGATCGCCGC TGTGTTCGGG
    ATTGTCATTT ACAGGGTGGT GACCGTCAGC ACTTTCGCAG CCTTTAAGTG GGCATTAATC
    AGGAATAATT CTCAGGTTGC AACTACAGGA ACTGCTGTGT GCATCAATTT CTGCATCATT
    ATGCTGCTGA ATGTGCTCTA TGAAAAGGTT GCTCTTCTTC TGACAAATTT AGAACAGCCT
    CGCACAGAGT CCGAGTGGGA GAACAGCTTC ACCCTGAAAA TGTTTCTTTT TCAGTTTGTC
    AATCTGAACA GCTCCACATT TTACATCGCG TTCTTCCTCG GAAGATTCAC AGGACACCCA
    GGTGCCTACT TGAGGCTGAT AAACAGGTGG AGACTAGAAG AGTGCCACCC TAGTGGATGC
    CTTATTGATC TCTGTATGCA AATGGGTATT ATAATGGTGC TAAAGCAGAC CTGGAATAAT
    TTCATGGAAC TTGGCTACCC GTTAATTCAG AATTGGTGGA CTAGAAGAAA AGTACGACAA
    GAACATGGGC CTGAAAGGAA AATAAGTTTC CCACAATGGG AAAAGGACTA CAACCTTCAG
    CCGATGAATG CCTACGGACT CTTTGATGAA TACTTAGAAA TGATTCTTCA GTTTGGATTT
    ACAACTATCT TTGTGGCAGC TTTTCCCCTA GCACCACTTC TGGCCTTACT GAATAATATA
    ATTGAAATTC GACTTGACGC TTATAAATTT GTCACACAGT GGAGGCGACC TTTAGCTTCA
    AGGGCCAAAG ATATAGGAAT TTGGTATGGG ATTCTTGAAG GCATTGGAAT TCTCTCCGTT
    ATTACAAATG CATTTGTGAT AGCGATCACA TCTGACTTTA TCCCTCGCTT GGTATATGCT
    TATAAGTATG GACCTTGTGC GGGCCAAGGA GAAGCTGGGC AAAAGTGCAT GGTTGGCTAT
    GTGAATGCCA GCTTGTCTGT ATTCCGAATT TCTGACTTTG AGAACAGATC TGAACCTGAG
    TCTGATGGCA GTGAGTTCTC GGGGACTCCT CTCAAGTACT GTAGATACCG AGACTACCGT
    GACCCTCCTC ATTCACTGGT GCCCTACGGC TACACACTAC AGTTCTGGCA CGTCCTAGCA
    GCCCGACTGG CTTTTATCAT TGTGTTCGAG CACCTCGTGT TTTGTATAAA GCACCTCATT
    TCATATCTGA TCCCAGACCT CCCAAAAGAT CTAAGGGATC GAATGAGAAG AGAGAAGTAC
    TTGATTCAGG AGATGATGTA TGAAGCAGAA CTGGAGCGTC TCCAGAAGGA ACGAAAGGAG
    AGGAAGAAAA ATGGAAAAGC ACACCACAAT GAGTGGCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19432
    Clone ID Related Accession (Same CDS sequence) XM_024991753.1 , XM_024991748.1 , XM_024991754.1 , XM_005206594.3 , XM_005206594.4
    Accession Version XM_024991753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847521.1
    CDS482..3349
    Translation

    Target ORF information:

    RefSeq Version XM_024991753.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19432
    Clone ID Related Accession (Same CDS sequence) XM_024991753.1 , XM_024991748.1 , XM_024991754.1 , XM_005206594.3 , XM_005206594.4
    Accession Version XM_024991748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847516.1
    CDS625..3492
    Translation

    Target ORF information:

    RefSeq Version XM_024991748.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19432
    Clone ID Related Accession (Same CDS sequence) XM_024991753.1 , XM_024991748.1 , XM_024991754.1 , XM_005206594.3 , XM_005206594.4
    Accession Version XM_024991754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847522.1
    CDS483..3350
    Translation

    Target ORF information:

    RefSeq Version XM_024991754.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19432
    Clone ID Related Accession (Same CDS sequence) XM_024991753.1 , XM_024991748.1 , XM_024991754.1 , XM_005206594.3 , XM_005206594.4
    Accession Version XM_005206594.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206651.1
    CDS953..3820
    Translation

    Target ORF information:

    RefSeq Version XM_005206594.3
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206594.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19432
    Clone ID Related Accession (Same CDS sequence) XM_024991753.1 , XM_024991748.1 , XM_024991754.1 , XM_005206594.3 , XM_005206594.4
    Accession Version XM_005206594.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206594.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206651.1
    CDS1248..4115
    Translation

    Target ORF information:

    RefSeq Version XM_005206594.4
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206594.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGAAGGA 
    GGTGTGGATT TGCAAAGCTA CCAGCTGGAT ATGCAAATAC TACCTGACGG GCCAAGGAGT
    GATGTGGACT TTTCAGAGAT TCTTAATGCA ATACAAGAAA TGGCCAAGGA TGTCAATATT
    CTTTTTGATG AATTAGAAGC TGTCAACAGT CCTTGCAAAG ATGACGACTC TCTCCTTCAC
    CCTGGAAATC TTACCAGTAC TTCAGATGAT GCTAGCAGGT TGGAAGCCGG GGGAGAGACA
    GTACCAGAAA AAAACAAATT AAATGGCCTT TACTTCAGAG ACGGAAAATG TCGAATTGAC
    TACATTCTTG TGTACAGAAA ATCCAACCCC CAGATGGAAA AGAGAGAAGT ATTTGAAAGA
    AATATTAGAG CAGAAGGATT ACAAATGGAG AAAGAGTCCT CTCTAATAAA TAGTGACATT
    ATCTTTGTGA AGTTGCATGC CCCATGGGAA GTGCTTGGAA GATATGCAGA ACAAATGAAT
    GTAAGAATGC CTTTCAGGAG AAAAATCTAT TACCTGCCCC GCCGGTACAA GTTCATGAGC
    AGGATCGATA AACAAATAAG CAGGTTTCGG AGATGGTTAC CTAAGAAGCC AATGAGGCTG
    GACAAGGAGA CACTGCCAGA CCTGGAGGAG AATGACTGCT ACACTGCCCC TTTCAGCCAG
    CAAAGGATCC ATCACTTCAT CATACACAAC AAAGACACTT TCTTCAACAA TGCCACAAGA
    AGTAGAATTG TACATCATAT TTTACAAAGA ATAAAGTATG AAGAAGGAAA AAACAAAATT
    GGTCTGAATC GTTTGCTTAC TAATGGCTCC TATGAAGCTG CATTTCCTCT GCACGAGGGA
    AGTTACAGAA GTAAAAACTC CATCAGAACC CACGGAGCGG TAAACCACCG ACATCTGCTC
    TATGAGTGCT GGGCGTCCTG GGGTGTGTGG TACAAATACC AGCCTTTGGA TCTTGTAAGG
    CGGTACTTTG GAGAGAAGAT TGGGTTGTAC TTTGCCTGGT TGGGCTGGTA CACCGGCATG
    CTCTTCCCAG CTGCCTTCAT TGGATTGTTT GTCTTTTTGT ATGGCGTCAT CACTCTGGAT
    CACTGCCAAG TCAGTAAAGA AGTCTGCCAA GCTACAGATA TCATCATGTG TCCTGTGTGT
    GATAAATACT GTCCATTCAT GAGGCTGTCA GACAGCTGCG TTTATGCCAA GGTAACCCAT
    CTTTTTGACA ATGGAGCCAC TGTTTTCTTT GCTGTTTTCA TGGCAGTCTG GGCAACAGTT
    TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA GTAATTGCTT ATGACTGGGA TTTGATAGAC
    TGGGAAGAAG AGGAGGAAGA AATACGGCCC CAGTTTGAAG CCAAGTATTC CAAGAAAGAG
    AGGATGAATC CCATTTCCGG CAAGCCAGAA CCATATCAAG CATTTGCAGA TAAATGCAGC
    CGACTTATTG TTTCTGCATC TGGAATATTT TTTATGATCT GCGTGGTGAT CGCCGCTGTG
    TTCGGGATTG TCATTTACAG GGTGGTGACC GTCAGCACTT TCGCAGCCTT TAAGTGGGCA
    TTAATCAGGA ATAATTCTCA GGTTGCAACT ACAGGAACTG CTGTGTGCAT CAATTTCTGC
    ATCATTATGC TGCTGAATGT GCTCTATGAA AAGGTTGCTC TTCTTCTGAC AAATTTAGAA
    CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC AGCTTCACCC TGAAAATGTT TCTTTTTCAG
    TTTGTCAATC TGAACAGCTC CACATTTTAC ATCGCGTTCT TCCTCGGAAG ATTCACAGGA
    CACCCAGGTG CCTACTTGAG GCTGATAAAC AGGTGGAGAC TAGAAGAGTG CCACCCTAGT
    GGATGCCTTA TTGATCTCTG TATGCAAATG GGTATTATAA TGGTGCTAAA GCAGACCTGG
    AATAATTTCA TGGAACTTGG CTACCCGTTA ATTCAGAATT GGTGGACTAG AAGAAAAGTA
    CGACAAGAAC ATGGGCCTGA AAGGAAAATA AGTTTCCCAC AATGGGAAAA GGACTACAAC
    CTTCAGCCGA TGAATGCCTA CGGACTCTTT GATGAATACT TAGAAATGAT TCTTCAGTTT
    GGATTTACAA CTATCTTTGT GGCAGCTTTT CCCCTAGCAC CACTTCTGGC CTTACTGAAT
    AATATAATTG AAATTCGACT TGACGCTTAT AAATTTGTCA CACAGTGGAG GCGACCTTTA
    GCTTCAAGGG CCAAAGATAT AGGAATTTGG TATGGGATTC TTGAAGGCAT TGGAATTCTC
    TCCGTTATTA CAAATGCATT TGTGATAGCG ATCACATCTG ACTTTATCCC TCGCTTGGTA
    TATGCTTATA AGTATGGACC TTGTGCGGGC CAAGGAGAAG CTGGGCAAAA GTGCATGGTT
    GGCTATGTGA ATGCCAGCTT GTCTGTATTC CGAATTTCTG ACTTTGAGAA CAGATCTGAA
    CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG ACTCCTCTCA AGTACTGTAG ATACCGAGAC
    TACCGTGACC CTCCTCATTC ACTGGTGCCC TACGGCTACA CACTACAGTT CTGGCACGTC
    CTAGCAGCCC GACTGGCTTT TATCATTGTG TTCGAGCACC TCGTGTTTTG TATAAAGCAC
    CTCATTTCAT ATCTGATCCC AGACCTCCCA AAAGATCTAA GGGATCGAAT GAGAAGAGAG
    AAGTACTTGA TTCAGGAGAT GATGTATGAA GCAGAACTGG AGCGTCTCCA GAAGGAACGA
    AAGGAGAGGA AGAAAAATGG AAAAGCACAC CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19434
    Clone ID Related Accession (Same CDS sequence) NM_001102050.1 , XM_024991752.1 , XM_005206596.4 , XM_005206596.3
    Accession Version XM_024991752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGTGGCC 
    AAGGATGTCA ATATTCTTTT TGATGAATTA GAAGCTGTCA ACAGTCCTTG CAAAGATGAC
    GACTCTCTCC TTCACCCTGG AAATCTTACC AGTACTTCAG ATGATGCTAG CAGGTTGGAA
    GCCGGGGGAG AGACAGTACC AGAAAAAAAC AAATTAAATG GCCTTTACTT CAGAGACGGA
    AAATGTCGAA TTGACTACAT TCTTGTGTAC AGAAAATCCA ACCCCCAGAT GGAAAAGAGA
    GAAGTATTTG AAAGAAATAT TAGAGCAGAA GGATTACAAA TGGAGAAAGA GTCCTCTCTA
    ATAAATAGTG ACATTATCTT TGTGAAGTTG CATGCCCCAT GGGAAGTGCT TGGAAGATAT
    GCAGAACAAA TGAATGTAAG AATGCCTTTC AGGAGAAAAA TCTATTACCT GCCCCGCCGG
    TACAAGTTCA TGAGCAGGAT CGATAAACAA ATAAGCAGGT TTCGGAGATG GTTACCTAAG
    AAGCCAATGA GGCTGGACAA GGAGACACTG CCAGACCTGG AGGAGAATGA CTGCTACACT
    GCCCCTTTCA GCCAGCAAAG GATCCATCAC TTCATCATAC ACAACAAAGA CACTTTCTTC
    AACAATGCCA CAAGAAGTAG AATTGTACAT CATATTTTAC AAAGAATAAA GTATGAAGAA
    GGAAAAAACA AAATTGGTCT GAATCGTTTG CTTACTAATG GCTCCTATGA AGCTGCATTT
    CCTCTGCACG AGGGAAGTTA CAGAAGTAAA AACTCCATCA GAACCCACGG AGCGGTAAAC
    CACCGACATC TGCTCTATGA GTGCTGGGCG TCCTGGGGTG TGTGGTACAA ATACCAGCCT
    TTGGATCTTG TAAGGCGGTA CTTTGGAGAG AAGATTGGGT TGTACTTTGC CTGGTTGGGC
    TGGTACACCG GCATGCTCTT CCCAGCTGCC TTCATTGGAT TGTTTGTCTT TTTGTATGGC
    GTCATCACTC TGGATCACTG CCAAGTCAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847520.1
    CDS364..3126
    Translation

    Target ORF information:

    RefSeq Version XM_024991752.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGTGGCC 
    AAGGATGTCA ATATTCTTTT TGATGAATTA GAAGCTGTCA ACAGTCCTTG CAAAGATGAC
    GACTCTCTCC TTCACCCTGG AAATCTTACC AGTACTTCAG ATGATGCTAG CAGGTTGGAA
    GCCGGGGGAG AGACAGTACC AGAAAAAAAC AAATTAAATG GCCTTTACTT CAGAGACGGA
    AAATGTCGAA TTGACTACAT TCTTGTGTAC AGAAAATCCA ACCCCCAGAT GGAAAAGAGA
    GAAGTATTTG AAAGAAATAT TAGAGCAGAA GGATTACAAA TGGAGAAAGA GTCCTCTCTA
    ATAAATAGTG ACATTATCTT TGTGAAGTTG CATGCCCCAT GGGAAGTGCT TGGAAGATAT
    GCAGAACAAA TGAATGTAAG AATGCCTTTC AGGAGAAAAA TCTATTACCT GCCCCGCCGG
    TACAAGTTCA TGAGCAGGAT CGATAAACAA ATAAGCAGGT TTCGGAGATG GTTACCTAAG
    AAGCCAATGA GGCTGGACAA GGAGACACTG CCAGACCTGG AGGAGAATGA CTGCTACACT
    GCCCCTTTCA GCCAGCAAAG GATCCATCAC TTCATCATAC ACAACAAAGA CACTTTCTTC
    AACAATGCCA CAAGAAGTAG AATTGTACAT CATATTTTAC AAAGAATAAA GTATGAAGAA
    GGAAAAAACA AAATTGGTCT GAATCGTTTG CTTACTAATG GCTCCTATGA AGCTGCATTT
    CCTCTGCACG AGGGAAGTTA CAGAAGTAAA AACTCCATCA GAACCCACGG AGCGGTAAAC
    CACCGACATC TGCTCTATGA GTGCTGGGCG TCCTGGGGTG TGTGGTACAA ATACCAGCCT
    TTGGATCTTG TAAGGCGGTA CTTTGGAGAG AAGATTGGGT TGTACTTTGC CTGGTTGGGC
    TGGTACACCG GCATGCTCTT CCCAGCTGCC TTCATTGGAT TGTTTGTCTT TTTGTATGGC
    GTCATCACTC TGGATCACTG CCAAGTCAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19434
    Clone ID Related Accession (Same CDS sequence) NM_001102050.1 , XM_024991752.1 , XM_005206596.4 , XM_005206596.3
    Accession Version XM_005206596.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206596.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGTGGCC 
    AAGGATGTCA ATATTCTTTT TGATGAATTA GAAGCTGTCA ACAGTCCTTG CAAAGATGAC
    GACTCTCTCC TTCACCCTGG AAATCTTACC AGTACTTCAG ATGATGCTAG CAGGTTGGAA
    GCCGGGGGAG AGACAGTACC AGAAAAAAAC AAATTAAATG GCCTTTACTT CAGAGACGGA
    AAATGTCGAA TTGACTACAT TCTTGTGTAC AGAAAATCCA ACCCCCAGAT GGAAAAGAGA
    GAAGTATTTG AAAGAAATAT TAGAGCAGAA GGATTACAAA TGGAGAAAGA GTCCTCTCTA
    ATAAATAGTG ACATTATCTT TGTGAAGTTG CATGCCCCAT GGGAAGTGCT TGGAAGATAT
    GCAGAACAAA TGAATGTAAG AATGCCTTTC AGGAGAAAAA TCTATTACCT GCCCCGCCGG
    TACAAGTTCA TGAGCAGGAT CGATAAACAA ATAAGCAGGT TTCGGAGATG GTTACCTAAG
    AAGCCAATGA GGCTGGACAA GGAGACACTG CCAGACCTGG AGGAGAATGA CTGCTACACT
    GCCCCTTTCA GCCAGCAAAG GATCCATCAC TTCATCATAC ACAACAAAGA CACTTTCTTC
    AACAATGCCA CAAGAAGTAG AATTGTACAT CATATTTTAC AAAGAATAAA GTATGAAGAA
    GGAAAAAACA AAATTGGTCT GAATCGTTTG CTTACTAATG GCTCCTATGA AGCTGCATTT
    CCTCTGCACG AGGGAAGTTA CAGAAGTAAA AACTCCATCA GAACCCACGG AGCGGTAAAC
    CACCGACATC TGCTCTATGA GTGCTGGGCG TCCTGGGGTG TGTGGTACAA ATACCAGCCT
    TTGGATCTTG TAAGGCGGTA CTTTGGAGAG AAGATTGGGT TGTACTTTGC CTGGTTGGGC
    TGGTACACCG GCATGCTCTT CCCAGCTGCC TTCATTGGAT TGTTTGTCTT TTTGTATGGC
    GTCATCACTC TGGATCACTG CCAAGTCAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206653.1
    CDS599..3361
    Translation

    Target ORF information:

    RefSeq Version XM_005206596.4
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206596.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGTGGCC 
    AAGGATGTCA ATATTCTTTT TGATGAATTA GAAGCTGTCA ACAGTCCTTG CAAAGATGAC
    GACTCTCTCC TTCACCCTGG AAATCTTACC AGTACTTCAG ATGATGCTAG CAGGTTGGAA
    GCCGGGGGAG AGACAGTACC AGAAAAAAAC AAATTAAATG GCCTTTACTT CAGAGACGGA
    AAATGTCGAA TTGACTACAT TCTTGTGTAC AGAAAATCCA ACCCCCAGAT GGAAAAGAGA
    GAAGTATTTG AAAGAAATAT TAGAGCAGAA GGATTACAAA TGGAGAAAGA GTCCTCTCTA
    ATAAATAGTG ACATTATCTT TGTGAAGTTG CATGCCCCAT GGGAAGTGCT TGGAAGATAT
    GCAGAACAAA TGAATGTAAG AATGCCTTTC AGGAGAAAAA TCTATTACCT GCCCCGCCGG
    TACAAGTTCA TGAGCAGGAT CGATAAACAA ATAAGCAGGT TTCGGAGATG GTTACCTAAG
    AAGCCAATGA GGCTGGACAA GGAGACACTG CCAGACCTGG AGGAGAATGA CTGCTACACT
    GCCCCTTTCA GCCAGCAAAG GATCCATCAC TTCATCATAC ACAACAAAGA CACTTTCTTC
    AACAATGCCA CAAGAAGTAG AATTGTACAT CATATTTTAC AAAGAATAAA GTATGAAGAA
    GGAAAAAACA AAATTGGTCT GAATCGTTTG CTTACTAATG GCTCCTATGA AGCTGCATTT
    CCTCTGCACG AGGGAAGTTA CAGAAGTAAA AACTCCATCA GAACCCACGG AGCGGTAAAC
    CACCGACATC TGCTCTATGA GTGCTGGGCG TCCTGGGGTG TGTGGTACAA ATACCAGCCT
    TTGGATCTTG TAAGGCGGTA CTTTGGAGAG AAGATTGGGT TGTACTTTGC CTGGTTGGGC
    TGGTACACCG GCATGCTCTT CCCAGCTGCC TTCATTGGAT TGTTTGTCTT TTTGTATGGC
    GTCATCACTC TGGATCACTG CCAAGTCAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19434
    Clone ID Related Accession (Same CDS sequence) NM_001102050.1 , XM_024991752.1 , XM_005206596.4 , XM_005206596.3
    Accession Version XM_005206596.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206596.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGTGGCC 
    AAGGATGTCA ATATTCTTTT TGATGAATTA GAAGCTGTCA ACAGTCCTTG CAAAGATGAC
    GACTCTCTCC TTCACCCTGG AAATCTTACC AGTACTTCAG ATGATGCTAG CAGGTTGGAA
    GCCGGGGGAG AGACAGTACC AGAAAAAAAC AAATTAAATG GCCTTTACTT CAGAGACGGA
    AAATGTCGAA TTGACTACAT TCTTGTGTAC AGAAAATCCA ACCCCCAGAT GGAAAAGAGA
    GAAGTATTTG AAAGAAATAT TAGAGCAGAA GGATTACAAA TGGAGAAAGA GTCCTCTCTA
    ATAAATAGTG ACATTATCTT TGTGAAGTTG CATGCCCCAT GGGAAGTGCT TGGAAGATAT
    GCAGAACAAA TGAATGTAAG AATGCCTTTC AGGAGAAAAA TCTATTACCT GCCCCGCCGG
    TACAAGTTCA TGAGCAGGAT CGATAAACAA ATAAGCAGGT TTCGGAGATG GTTACCTAAG
    AAGCCAATGA GGCTGGACAA GGAGACACTG CCAGACCTGG AGGAGAATGA CTGCTACACT
    GCCCCTTTCA GCCAGCAAAG GATCCATCAC TTCATCATAC ACAACAAAGA CACTTTCTTC
    AACAATGCCA CAAGAAGTAG AATTGTACAT CATATTTTAC AAAGAATAAA GTATGAAGAA
    GGAAAAAACA AAATTGGTCT GAATCGTTTG CTTACTAATG GCTCCTATGA AGCTGCATTT
    CCTCTGCACG AGGGAAGTTA CAGAAGTAAA AACTCCATCA GAACCCACGG AGCGGTAAAC
    CACCGACATC TGCTCTATGA GTGCTGGGCG TCCTGGGGTG TGTGGTACAA ATACCAGCCT
    TTGGATCTTG TAAGGCGGTA CTTTGGAGAG AAGATTGGGT TGTACTTTGC CTGGTTGGGC
    TGGTACACCG GCATGCTCTT CCCAGCTGCC TTCATTGGAT TGTTTGTCTT TTTGTATGGC
    GTCATCACTC TGGATCACTG CCAAGTCAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206653.1
    CDS562..3324
    Translation

    Target ORF information:

    RefSeq Version XM_005206596.3
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206596.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGACGA GCTCTTCTGG AATCACTAAT GGGAGAACCA GAGTCTTCCA CCCAGTGGCC 
    AAGGATGTCA ATATTCTTTT TGATGAATTA GAAGCTGTCA ACAGTCCTTG CAAAGATGAC
    GACTCTCTCC TTCACCCTGG AAATCTTACC AGTACTTCAG ATGATGCTAG CAGGTTGGAA
    GCCGGGGGAG AGACAGTACC AGAAAAAAAC AAATTAAATG GCCTTTACTT CAGAGACGGA
    AAATGTCGAA TTGACTACAT TCTTGTGTAC AGAAAATCCA ACCCCCAGAT GGAAAAGAGA
    GAAGTATTTG AAAGAAATAT TAGAGCAGAA GGATTACAAA TGGAGAAAGA GTCCTCTCTA
    ATAAATAGTG ACATTATCTT TGTGAAGTTG CATGCCCCAT GGGAAGTGCT TGGAAGATAT
    GCAGAACAAA TGAATGTAAG AATGCCTTTC AGGAGAAAAA TCTATTACCT GCCCCGCCGG
    TACAAGTTCA TGAGCAGGAT CGATAAACAA ATAAGCAGGT TTCGGAGATG GTTACCTAAG
    AAGCCAATGA GGCTGGACAA GGAGACACTG CCAGACCTGG AGGAGAATGA CTGCTACACT
    GCCCCTTTCA GCCAGCAAAG GATCCATCAC TTCATCATAC ACAACAAAGA CACTTTCTTC
    AACAATGCCA CAAGAAGTAG AATTGTACAT CATATTTTAC AAAGAATAAA GTATGAAGAA
    GGAAAAAACA AAATTGGTCT GAATCGTTTG CTTACTAATG GCTCCTATGA AGCTGCATTT
    CCTCTGCACG AGGGAAGTTA CAGAAGTAAA AACTCCATCA GAACCCACGG AGCGGTAAAC
    CACCGACATC TGCTCTATGA GTGCTGGGCG TCCTGGGGTG TGTGGTACAA ATACCAGCCT
    TTGGATCTTG TAAGGCGGTA CTTTGGAGAG AAGATTGGGT TGTACTTTGC CTGGTTGGGC
    TGGTACACCG GCATGCTCTT CCCAGCTGCC TTCATTGGAT TGTTTGTCTT TTTGTATGGC
    GTCATCACTC TGGATCACTG CCAAGTCAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234388
    Clone ID Related Accession (Same CDS sequence) XM_024991749.1
    Accession Version XM_024991749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGT GGCCAAGGAT
    GTCAATATTC TTTTTGATGA ATTAGAAGCT GTCAACAGTC CTTGCAAAGA TGACGACTCT
    CTCCTTCACC CTGGAAATCT TACCAGTACT TCAGATGATG CTAGCAGGTT GGAAGCCGGG
    GGAGAGACAG TACCAGAAAA AAACAAATTA AATGGCCTTT ACTTCAGAGA CGGAAAATGT
    CGAATTGACT ACATTCTTGT GTACAGAAAA TCCAACCCCC AGATGGAAAA GAGAGAAGTA
    TTTGAAAGAA ATATTAGAGC AGAAGGATTA CAAATGGAGA AAGAGTCCTC TCTAATAAAT
    AGTGACATTA TCTTTGTGAA GTTGCATGCC CCATGGGAAG TGCTTGGAAG ATATGCAGAA
    CAAATGAATG TAAGAATGCC TTTCAGGAGA AAAATCTATT ACCTGCCCCG CCGGTACAAG
    TTCATGAGCA GGATCGATAA ACAAATAAGC AGGTTTCGGA GATGGTTACC TAAGAAGCCA
    ATGAGGCTGG ACAAGGAGAC ACTGCCAGAC CTGGAGGAGA ATGACTGCTA CACTGCCCCT
    TTCAGCCAGC AAAGGATCCA TCACTTCATC ATACACAACA AAGACACTTT CTTCAACAAT
    GCCACAAGAA GTAGAATTGT ACATCATATT TTACAAAGAA TAAAGTATGA AGAAGGAAAA
    AACAAAATTG GTCTGAATCG TTTGCTTACT AATGGCTCCT ATGAAGCTGC ATTTCCTCTG
    CACGAGGGAA GTTACAGAAG TAAAAACTCC ATCAGAACCC ACGGAGCGGT AAACCACCGA
    CATCTGCTCT ATGAGTGCTG GGCGTCCTGG GGTGTGTGGT ACAAATACCA GCCTTTGGAT
    CTTGTAAGGC GGTACTTTGG AGAGAAGATT GGGTTGTACT TTGCCTGGTT GGGCTGGTAC
    ACCGGCATGC TCTTCCCAGC TGCCTTCATT GGATTGTTTG TCTTTTTGTA TGGCGTCATC
    ACTCTGGATC ACTGCCAAGT CAGTAAAGAA GTCTGCCAAG CTACAGATAT CATCATGTGT
    CCTGTGTGTG ATAAATACTG TCCATTCATG AGGCTGTCAG ACAGCTGCGT TTATGCCAAG
    GTAACCCATC TTTTTGACAA TGGAGCCACT GTTTTCTTTG CTGTTTTCAT GGCAGTCTGG
    GCAACAGTTT TCCTAGAGTT TTGGAAGAGA CGGAGAGCAG TAATTGCTTA TGACTGGGAT
    TTGATAGACT GGGAAGAAGA GGAGGAAGAA ATACGGCCCC AGTTTGAAGC CAAGTATTCC
    AAGAAAGAGA GGATGAATCC CATTTCCGGC AAGCCAGAAC CATATCAAGC ATTTGCAGAT
    AAATGCAGCC GACTTATTGT TTCTGCATCT GGAATATTTT TTATGATCTG CGTGGTGATC
    GCCGCTGTGT TCGGGATTGT CATTTACAGG GTGGTGACCG TCAGCACTTT CGCAGCCTTT
    AAGTGGGCAT TAATCAGGAA TAATTCTCAG GTTGCAACTA CAGGAACTGC TGTGTGCATC
    AATTTCTGCA TCATTATGCT GCTGAATGTG CTCTATGAAA AGGTTGCTCT TCTTCTGACA
    AATTTAGAAC AGCCTCGCAC AGAGTCCGAG TGGGAGAACA GCTTCACCCT GAAAATGTTT
    CTTTTTCAGT TTGTCAATCT GAACAGCTCC ACATTTTACA TCGCGTTCTT CCTCGGAAGA
    TTCACAGGAC ACCCAGGTGC CTACTTGAGG CTGATAAACA GGTGGAGACT AGAAGAGTGC
    CACCCTAGTG GATGCCTTAT TGATCTCTGT ATGCAAATGG GTATTATAAT GGTGCTAAAG
    CAGACCTGGA ATAATTTCAT GGAACTTGGC TACCCGTTAA TTCAGAATTG GTGGACTAGA
    AGAAAAGTAC GACAAGAACA TGGGCCTGAA AGGAAAATAA GTTTCCCACA ATGGGAAAAG
    GACTACAACC TTCAGCCGAT GAATGCCTAC GGACTCTTTG ATGAATACTT AGAAATGATT
    CTTCAGTTTG GATTTACAAC TATCTTTGTG GCAGCTTTTC CCCTAGCACC ACTTCTGGCC
    TTACTGAATA ATATAATTGA AATTCGACTT GACGCTTATA AATTTGTCAC ACAGTGGAGG
    CGACCTTTAG CTTCAAGGGC CAAAGATATA GGAATTTGGT ATGGGATTCT TGAAGGCATT
    GGAATTCTCT CCGTTATTAC AAATGCATTT GTGATAGCGA TCACATCTGA CTTTATCCCT
    CGCTTGGTAT ATGCTTATAA GTATGGACCT TGTGCGGGCC AAGGAGAAGC TGGGCAAAAG
    TGCATGGTTG GCTATGTGAA TGCCAGCTTG TCTGTATTCC GAATTTCTGA CTTTGAGAAC
    AGATCTGAAC CTGAGTCTGA TGGCAGTGAG TTCTCGGGGA CTCCTCTCAA GTACTGTAGA
    TACCGAGACT ACCGTGACCC TCCTCATTCA CTGGTGCCCT ACGGCTACAC ACTACAGTTC
    TGGCACGTCC TAGCAGCCCG ACTGGCTTTT ATCATTGTGT TCGAGCACCT CGTGTTTTGT
    ATAAAGCACC TCATTTCATA TCTGATCCCA GACCTCCCAA AAGATCTAAG GGATCGAATG
    AGAAGAGAGA AGTACTTGAT TCAGGAGATG ATGTATGAAG CAGAACTGGA GCGTCTCCAG
    AAGGAACGAA AGGAGAGGAA GAAAAATGGA AAAGCACACC ACAATGAGTG GCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847517.1
    CDS90..2906
    Translation

    Target ORF information:

    RefSeq Version XM_024991749.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGT GGCCAAGGAT
    GTCAATATTC TTTTTGATGA ATTAGAAGCT GTCAACAGTC CTTGCAAAGA TGACGACTCT
    CTCCTTCACC CTGGAAATCT TACCAGTACT TCAGATGATG CTAGCAGGTT GGAAGCCGGG
    GGAGAGACAG TACCAGAAAA AAACAAATTA AATGGCCTTT ACTTCAGAGA CGGAAAATGT
    CGAATTGACT ACATTCTTGT GTACAGAAAA TCCAACCCCC AGATGGAAAA GAGAGAAGTA
    TTTGAAAGAA ATATTAGAGC AGAAGGATTA CAAATGGAGA AAGAGTCCTC TCTAATAAAT
    AGTGACATTA TCTTTGTGAA GTTGCATGCC CCATGGGAAG TGCTTGGAAG ATATGCAGAA
    CAAATGAATG TAAGAATGCC TTTCAGGAGA AAAATCTATT ACCTGCCCCG CCGGTACAAG
    TTCATGAGCA GGATCGATAA ACAAATAAGC AGGTTTCGGA GATGGTTACC TAAGAAGCCA
    ATGAGGCTGG ACAAGGAGAC ACTGCCAGAC CTGGAGGAGA ATGACTGCTA CACTGCCCCT
    TTCAGCCAGC AAAGGATCCA TCACTTCATC ATACACAACA AAGACACTTT CTTCAACAAT
    GCCACAAGAA GTAGAATTGT ACATCATATT TTACAAAGAA TAAAGTATGA AGAAGGAAAA
    AACAAAATTG GTCTGAATCG TTTGCTTACT AATGGCTCCT ATGAAGCTGC ATTTCCTCTG
    CACGAGGGAA GTTACAGAAG TAAAAACTCC ATCAGAACCC ACGGAGCGGT AAACCACCGA
    CATCTGCTCT ATGAGTGCTG GGCGTCCTGG GGTGTGTGGT ACAAATACCA GCCTTTGGAT
    CTTGTAAGGC GGTACTTTGG AGAGAAGATT GGGTTGTACT TTGCCTGGTT GGGCTGGTAC
    ACCGGCATGC TCTTCCCAGC TGCCTTCATT GGATTGTTTG TCTTTTTGTA TGGCGTCATC
    ACTCTGGATC ACTGCCAAGT CAGTAAAGAA GTCTGCCAAG CTACAGATAT CATCATGTGT
    CCTGTGTGTG ATAAATACTG TCCATTCATG AGGCTGTCAG ACAGCTGCGT TTATGCCAAG
    GTAACCCATC TTTTTGACAA TGGAGCCACT GTTTTCTTTG CTGTTTTCAT GGCAGTCTGG
    GCAACAGTTT TCCTAGAGTT TTGGAAGAGA CGGAGAGCAG TAATTGCTTA TGACTGGGAT
    TTGATAGACT GGGAAGAAGA GGAGGAAGAA ATACGGCCCC AGTTTGAAGC CAAGTATTCC
    AAGAAAGAGA GGATGAATCC CATTTCCGGC AAGCCAGAAC CATATCAAGC ATTTGCAGAT
    AAATGCAGCC GACTTATTGT TTCTGCATCT GGAATATTTT TTATGATCTG CGTGGTGATC
    GCCGCTGTGT TCGGGATTGT CATTTACAGG GTGGTGACCG TCAGCACTTT CGCAGCCTTT
    AAGTGGGCAT TAATCAGGAA TAATTCTCAG GTTGCAACTA CAGGAACTGC TGTGTGCATC
    AATTTCTGCA TCATTATGCT GCTGAATGTG CTCTATGAAA AGGTTGCTCT TCTTCTGACA
    AATTTAGAAC AGCCTCGCAC AGAGTCCGAG TGGGAGAACA GCTTCACCCT GAAAATGTTT
    CTTTTTCAGT TTGTCAATCT GAACAGCTCC ACATTTTACA TCGCGTTCTT CCTCGGAAGA
    TTCACAGGAC ACCCAGGTGC CTACTTGAGG CTGATAAACA GGTGGAGACT AGAAGAGTGC
    CACCCTAGTG GATGCCTTAT TGATCTCTGT ATGCAAATGG GTATTATAAT GGTGCTAAAG
    CAGACCTGGA ATAATTTCAT GGAACTTGGC TACCCGTTAA TTCAGAATTG GTGGACTAGA
    AGAAAAGTAC GACAAGAACA TGGGCCTGAA AGGAAAATAA GTTTCCCACA ATGGGAAAAG
    GACTACAACC TTCAGCCGAT GAATGCCTAC GGACTCTTTG ATGAATACTT AGAAATGATT
    CTTCAGTTTG GATTTACAAC TATCTTTGTG GCAGCTTTTC CCCTAGCACC ACTTCTGGCC
    TTACTGAATA ATATAATTGA AATTCGACTT GACGCTTATA AATTTGTCAC ACAGTGGAGG
    CGACCTTTAG CTTCAAGGGC CAAAGATATA GGAATTTGGT ATGGGATTCT TGAAGGCATT
    GGAATTCTCT CCGTTATTAC AAATGCATTT GTGATAGCGA TCACATCTGA CTTTATCCCT
    CGCTTGGTAT ATGCTTATAA GTATGGACCT TGTGCGGGCC AAGGAGAAGC TGGGCAAAAG
    TGCATGGTTG GCTATGTGAA TGCCAGCTTG TCTGTATTCC GAATTTCTGA CTTTGAGAAC
    AGATCTGAAC CTGAGTCTGA TGGCAGTGAG TTCTCGGGGA CTCCTCTCAA GTACTGTAGA
    TACCGAGACT ACCGTGACCC TCCTCATTCA CTGGTGCCCT ACGGCTACAC ACTACAGTTC
    TGGCACGTCC TAGCAGCCCG ACTGGCTTTT ATCATTGTGT TCGAGCACCT CGTGTTTTGT
    ATAAAGCACC TCATTTCATA TCTGATCCCA GACCTCCCAA AAGATCTAAG GGATCGAATG
    AGAAGAGAGA AGTACTTGAT TCAGGAGATG ATGTATGAAG CAGAACTGGA GCGTCTCCAG
    AAGGAACGAA AGGAGAGGAA GAAAAATGGA AAAGCACACC ACAATGAGTG GCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234389
    Clone ID Related Accession (Same CDS sequence) XM_024991750.1
    Accession Version XM_024991750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2778bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA 
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGG CGGTACTTTG GAGAGAAGAT TGGGTTGTAC
    TTTGCCTGGT TGGGCTGGTA CACCGGCATG CTCTTCCCAG CTGCCTTCAT TGGATTGTTT
    GTCTTTTTGT ATGGCGTCAT CACTCTGGAT CACTGCCAAG TCAGTAAAGA AGTCTGCCAA
    GCTACAGATA TCATCATGTG TCCTGTGTGT GATAAATACT GTCCATTCAT GAGGCTGTCA
    GACAGCTGCG TTTATGCCAA GGTAACCCAT CTTTTTGACA ATGGAGCCAC TGTTTTCTTT
    GCTGTTTTCA TGGCAGTCTG GGCAACAGTT TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA
    GTAATTGCTT ATGACTGGGA TTTGATAGAC TGGGAAGAAG AGGAGGAAGA AATACGGCCC
    CAGTTTGAAG CCAAGTATTC CAAGAAAGAG AGGATGAATC CCATTTCCGG CAAGCCAGAA
    CCATATCAAG CATTTGCAGA TAAATGCAGC CGACTTATTG TTTCTGCATC TGGAATATTT
    TTTATGATCT GCGTGGTGAT CGCCGCTGTG TTCGGGATTG TCATTTACAG GGTGGTGACC
    GTCAGCACTT TCGCAGCCTT TAAGTGGGCA TTAATCAGGA ATAATTCTCA GGTTGCAACT
    ACAGGAACTG CTGTGTGCAT CAATTTCTGC ATCATTATGC TGCTGAATGT GCTCTATGAA
    AAGGTTGCTC TTCTTCTGAC AAATTTAGAA CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC
    AGCTTCACCC TGAAAATGTT TCTTTTTCAG TTTGTCAATC TGAACAGCTC CACATTTTAC
    ATCGCGTTCT TCCTCGGAAG ATTCACAGGA CACCCAGGTG CCTACTTGAG GCTGATAAAC
    AGGTGGAGAC TAGAAGAGTG CCACCCTAGT GGATGCCTTA TTGATCTCTG TATGCAAATG
    GGTATTATAA TGGTGCTAAA GCAGACCTGG AATAATTTCA TGGAACTTGG CTACCCGTTA
    ATTCAGAATT GGTGGACTAG AAGAAAAGTA CGACAAGAAC ATGGGCCTGA AAGGAAAATA
    AGTTTCCCAC AATGGGAAAA GGACTACAAC CTTCAGCCGA TGAATGCCTA CGGACTCTTT
    GATGAATACT TAGAAATGAT TCTTCAGTTT GGATTTACAA CTATCTTTGT GGCAGCTTTT
    CCCCTAGCAC CACTTCTGGC CTTACTGAAT AATATAATTG AAATTCGACT TGACGCTTAT
    AAATTTGTCA CACAGTGGAG GCGACCTTTA GCTTCAAGGG CCAAAGATAT AGGAATTTGG
    TATGGGATTC TTGAAGGCAT TGGAATTCTC TCCGTTATTA CAAATGCATT TGTGATAGCG
    ATCACATCTG ACTTTATCCC TCGCTTGGTA TATGCTTATA AGTATGGACC TTGTGCGGGC
    CAAGGAGAAG CTGGGCAAAA GTGCATGGTT GGCTATGTGA ATGCCAGCTT GTCTGTATTC
    CGAATTTCTG ACTTTGAGAA CAGATCTGAA CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG
    ACTCCTCTCA AGTACTGTAG ATACCGAGAC TACCGTGACC CTCCTCATTC ACTGGTGCCC
    TACGGCTACA CACTACAGTT CTGGCACGTC CTAGCAGCCC GACTGGCTTT TATCATTGTG
    TTCGAGCACC TCGTGTTTTG TATAAAGCAC CTCATTTCAT ATCTGATCCC AGACCTCCCA
    AAAGATCTAA GGGATCGAAT GAGAAGAGAG AAGTACTTGA TTCAGGAGAT GATGTATGAA
    GCAGAACTGG AGCGTCTCCA GAAGGAACGA AAGGAGAGGA AGAAAAATGG AAAAGCACAC
    CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847518.1
    CDS70..2847
    Translation

    Target ORF information:

    RefSeq Version XM_024991750.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA 
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGG CGGTACTTTG GAGAGAAGAT TGGGTTGTAC
    TTTGCCTGGT TGGGCTGGTA CACCGGCATG CTCTTCCCAG CTGCCTTCAT TGGATTGTTT
    GTCTTTTTGT ATGGCGTCAT CACTCTGGAT CACTGCCAAG TCAGTAAAGA AGTCTGCCAA
    GCTACAGATA TCATCATGTG TCCTGTGTGT GATAAATACT GTCCATTCAT GAGGCTGTCA
    GACAGCTGCG TTTATGCCAA GGTAACCCAT CTTTTTGACA ATGGAGCCAC TGTTTTCTTT
    GCTGTTTTCA TGGCAGTCTG GGCAACAGTT TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA
    GTAATTGCTT ATGACTGGGA TTTGATAGAC TGGGAAGAAG AGGAGGAAGA AATACGGCCC
    CAGTTTGAAG CCAAGTATTC CAAGAAAGAG AGGATGAATC CCATTTCCGG CAAGCCAGAA
    CCATATCAAG CATTTGCAGA TAAATGCAGC CGACTTATTG TTTCTGCATC TGGAATATTT
    TTTATGATCT GCGTGGTGAT CGCCGCTGTG TTCGGGATTG TCATTTACAG GGTGGTGACC
    GTCAGCACTT TCGCAGCCTT TAAGTGGGCA TTAATCAGGA ATAATTCTCA GGTTGCAACT
    ACAGGAACTG CTGTGTGCAT CAATTTCTGC ATCATTATGC TGCTGAATGT GCTCTATGAA
    AAGGTTGCTC TTCTTCTGAC AAATTTAGAA CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC
    AGCTTCACCC TGAAAATGTT TCTTTTTCAG TTTGTCAATC TGAACAGCTC CACATTTTAC
    ATCGCGTTCT TCCTCGGAAG ATTCACAGGA CACCCAGGTG CCTACTTGAG GCTGATAAAC
    AGGTGGAGAC TAGAAGAGTG CCACCCTAGT GGATGCCTTA TTGATCTCTG TATGCAAATG
    GGTATTATAA TGGTGCTAAA GCAGACCTGG AATAATTTCA TGGAACTTGG CTACCCGTTA
    ATTCAGAATT GGTGGACTAG AAGAAAAGTA CGACAAGAAC ATGGGCCTGA AAGGAAAATA
    AGTTTCCCAC AATGGGAAAA GGACTACAAC CTTCAGCCGA TGAATGCCTA CGGACTCTTT
    GATGAATACT TAGAAATGAT TCTTCAGTTT GGATTTACAA CTATCTTTGT GGCAGCTTTT
    CCCCTAGCAC CACTTCTGGC CTTACTGAAT AATATAATTG AAATTCGACT TGACGCTTAT
    AAATTTGTCA CACAGTGGAG GCGACCTTTA GCTTCAAGGG CCAAAGATAT AGGAATTTGG
    TATGGGATTC TTGAAGGCAT TGGAATTCTC TCCGTTATTA CAAATGCATT TGTGATAGCG
    ATCACATCTG ACTTTATCCC TCGCTTGGTA TATGCTTATA AGTATGGACC TTGTGCGGGC
    CAAGGAGAAG CTGGGCAAAA GTGCATGGTT GGCTATGTGA ATGCCAGCTT GTCTGTATTC
    CGAATTTCTG ACTTTGAGAA CAGATCTGAA CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG
    ACTCCTCTCA AGTACTGTAG ATACCGAGAC TACCGTGACC CTCCTCATTC ACTGGTGCCC
    TACGGCTACA CACTACAGTT CTGGCACGTC CTAGCAGCCC GACTGGCTTT TATCATTGTG
    TTCGAGCACC TCGTGTTTTG TATAAAGCAC CTCATTTCAT ATCTGATCCC AGACCTCCCA
    AAAGATCTAA GGGATCGAAT GAGAAGAGAG AAGTACTTGA TTCAGGAGAT GATGTATGAA
    GCAGAACTGG AGCGTCTCCA GAAGGAACGA AAGGAGAGGA AGAAAAATGG AAAAGCACAC
    CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234390
    Clone ID Related Accession (Same CDS sequence) XM_024991755.1
    Accession Version XM_024991755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGTTTTC TTCTCGACTG GCTTCATTTT TGCTGTTCTT CCAGGAGAAA AATCTATTAC 
    CTGCCCCGCC GGTACAAGTT CATGAGCAGG ATCGATAAAC AAATAAGCAG GTTTCGGAGA
    TGGTTACCTA AGAAGCCAAT GAGGCTGGAC AAGGAGACAC TGCCAGACCT GGAGGAGAAT
    GACTGCTACA CTGCCCCTTT CAGCCAGCAA AGGATCCATC ACTTCATCAT ACACAACAAA
    GACACTTTCT TCAACAATGC CACAAGAAGT AGAATTGTAC ATCATATTTT ACAAAGAATA
    AAGTATGAAG AAGGAAAAAA CAAAATTGGT CTGAATCGTT TGCTTACTAA TGGCTCCTAT
    GAAGCTGCAT TTCCTCTGCA CGAGGGAAGT TACAGAAGTA AAAACTCCAT CAGAACCCAC
    GGAGCGGTAA ACCACCGACA TCTGCTCTAT GAGTGCTGGG CGTCCTGGGG TGTGTGGTAC
    AAATACCAGC CTTTGGATCT TGTAAGGCGG TACTTTGGAG AGAAGATTGG GTTGTACTTT
    GCCTGGTTGG GCTGGTACAC CGGCATGCTC TTCCCAGCTG CCTTCATTGG ATTGTTTGTC
    TTTTTGTATG GCGTCATCAC TCTGGATCAC TGCCAAGTCA GTAAAGAAGT CTGCCAAGCT
    ACAGATATCA TCATGTGTCC TGTGTGTGAT AAATACTGTC CATTCATGAG GCTGTCAGAC
    AGCTGCGTTT ATGCCAAGGT AACCCATCTT TTTGACAATG GAGCCACTGT TTTCTTTGCT
    GTTTTCATGG CAGTCTGGGC AACAGTTTTC CTAGAGTTTT GGAAGAGACG GAGAGCAGTA
    ATTGCTTATG ACTGGGATTT GATAGACTGG GAAGAAGAGG AGGAAGAAAT ACGGCCCCAG
    TTTGAAGCCA AGTATTCCAA GAAAGAGAGG ATGAATCCCA TTTCCGGCAA GCCAGAACCA
    TATCAAGCAT TTGCAGATAA ATGCAGCCGA CTTATTGTTT CTGCATCTGG AATATTTTTT
    ATGATCTGCG TGGTGATCGC CGCTGTGTTC GGGATTGTCA TTTACAGGGT GGTGACCGTC
    AGCACTTTCG CAGCCTTTAA GTGGGCATTA ATCAGGAATA ATTCTCAGGT TGCAACTACA
    GGAACTGCTG TGTGCATCAA TTTCTGCATC ATTATGCTGC TGAATGTGCT CTATGAAAAG
    GTTGCTCTTC TTCTGACAAA TTTAGAACAG CCTCGCACAG AGTCCGAGTG GGAGAACAGC
    TTCACCCTGA AAATGTTTCT TTTTCAGTTT GTCAATCTGA ACAGCTCCAC ATTTTACATC
    GCGTTCTTCC TCGGAAGATT CACAGGACAC CCAGGTGCCT ACTTGAGGCT GATAAACAGG
    TGGAGACTAG AAGAGTGCCA CCCTAGTGGA TGCCTTATTG ATCTCTGTAT GCAAATGGGT
    ATTATAATGG TGCTAAAGCA GACCTGGAAT AATTTCATGG AACTTGGCTA CCCGTTAATT
    CAGAATTGGT GGACTAGAAG AAAAGTACGA CAAGAACATG GGCCTGAAAG GAAAATAAGT
    TTCCCACAAT GGGAAAAGGA CTACAACCTT CAGCCGATGA ATGCCTACGG ACTCTTTGAT
    GAATACTTAG AAATGATTCT TCAGTTTGGA TTTACAACTA TCTTTGTGGC AGCTTTTCCC
    CTAGCACCAC TTCTGGCCTT ACTGAATAAT ATAATTGAAA TTCGACTTGA CGCTTATAAA
    TTTGTCACAC AGTGGAGGCG ACCTTTAGCT TCAAGGGCCA AAGATATAGG AATTTGGTAT
    GGGATTCTTG AAGGCATTGG AATTCTCTCC GTTATTACAA ATGCATTTGT GATAGCGATC
    ACATCTGACT TTATCCCTCG CTTGGTATAT GCTTATAAGT ATGGACCTTG TGCGGGCCAA
    GGAGAAGCTG GGCAAAAGTG CATGGTTGGC TATGTGAATG CCAGCTTGTC TGTATTCCGA
    ATTTCTGACT TTGAGAACAG ATCTGAACCT GAGTCTGATG GCAGTGAGTT CTCGGGGACT
    CCTCTCAAGT ACTGTAGATA CCGAGACTAC CGTGACCCTC CTCATTCACT GGTGCCCTAC
    GGCTACACAC TACAGTTCTG GCACGTCCTA GCAGCCCGAC TGGCTTTTAT CATTGTGTTC
    GAGCACCTCG TGTTTTGTAT AAAGCACCTC ATTTCATATC TGATCCCAGA CCTCCCAAAA
    GATCTAAGGG ATCGAATGAG AAGAGAGAAG TACTTGATTC AGGAGATGAT GTATGAAGCA
    GAACTGGAGC GTCTCCAGAA GGAACGAAAG GAGAGGAAGA AAAATGGAAA AGCACACCAC
    AATGAGTGGC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847523.1
    CDS299..2653
    Translation

    Target ORF information:

    RefSeq Version XM_024991755.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGTTTTC TTCTCGACTG GCTTCATTTT TGCTGTTCTT CCAGGAGAAA AATCTATTAC 
    CTGCCCCGCC GGTACAAGTT CATGAGCAGG ATCGATAAAC AAATAAGCAG GTTTCGGAGA
    TGGTTACCTA AGAAGCCAAT GAGGCTGGAC AAGGAGACAC TGCCAGACCT GGAGGAGAAT
    GACTGCTACA CTGCCCCTTT CAGCCAGCAA AGGATCCATC ACTTCATCAT ACACAACAAA
    GACACTTTCT TCAACAATGC CACAAGAAGT AGAATTGTAC ATCATATTTT ACAAAGAATA
    AAGTATGAAG AAGGAAAAAA CAAAATTGGT CTGAATCGTT TGCTTACTAA TGGCTCCTAT
    GAAGCTGCAT TTCCTCTGCA CGAGGGAAGT TACAGAAGTA AAAACTCCAT CAGAACCCAC
    GGAGCGGTAA ACCACCGACA TCTGCTCTAT GAGTGCTGGG CGTCCTGGGG TGTGTGGTAC
    AAATACCAGC CTTTGGATCT TGTAAGGCGG TACTTTGGAG AGAAGATTGG GTTGTACTTT
    GCCTGGTTGG GCTGGTACAC CGGCATGCTC TTCCCAGCTG CCTTCATTGG ATTGTTTGTC
    TTTTTGTATG GCGTCATCAC TCTGGATCAC TGCCAAGTCA GTAAAGAAGT CTGCCAAGCT
    ACAGATATCA TCATGTGTCC TGTGTGTGAT AAATACTGTC CATTCATGAG GCTGTCAGAC
    AGCTGCGTTT ATGCCAAGGT AACCCATCTT TTTGACAATG GAGCCACTGT TTTCTTTGCT
    GTTTTCATGG CAGTCTGGGC AACAGTTTTC CTAGAGTTTT GGAAGAGACG GAGAGCAGTA
    ATTGCTTATG ACTGGGATTT GATAGACTGG GAAGAAGAGG AGGAAGAAAT ACGGCCCCAG
    TTTGAAGCCA AGTATTCCAA GAAAGAGAGG ATGAATCCCA TTTCCGGCAA GCCAGAACCA
    TATCAAGCAT TTGCAGATAA ATGCAGCCGA CTTATTGTTT CTGCATCTGG AATATTTTTT
    ATGATCTGCG TGGTGATCGC CGCTGTGTTC GGGATTGTCA TTTACAGGGT GGTGACCGTC
    AGCACTTTCG CAGCCTTTAA GTGGGCATTA ATCAGGAATA ATTCTCAGGT TGCAACTACA
    GGAACTGCTG TGTGCATCAA TTTCTGCATC ATTATGCTGC TGAATGTGCT CTATGAAAAG
    GTTGCTCTTC TTCTGACAAA TTTAGAACAG CCTCGCACAG AGTCCGAGTG GGAGAACAGC
    TTCACCCTGA AAATGTTTCT TTTTCAGTTT GTCAATCTGA ACAGCTCCAC ATTTTACATC
    GCGTTCTTCC TCGGAAGATT CACAGGACAC CCAGGTGCCT ACTTGAGGCT GATAAACAGG
    TGGAGACTAG AAGAGTGCCA CCCTAGTGGA TGCCTTATTG ATCTCTGTAT GCAAATGGGT
    ATTATAATGG TGCTAAAGCA GACCTGGAAT AATTTCATGG AACTTGGCTA CCCGTTAATT
    CAGAATTGGT GGACTAGAAG AAAAGTACGA CAAGAACATG GGCCTGAAAG GAAAATAAGT
    TTCCCACAAT GGGAAAAGGA CTACAACCTT CAGCCGATGA ATGCCTACGG ACTCTTTGAT
    GAATACTTAG AAATGATTCT TCAGTTTGGA TTTACAACTA TCTTTGTGGC AGCTTTTCCC
    CTAGCACCAC TTCTGGCCTT ACTGAATAAT ATAATTGAAA TTCGACTTGA CGCTTATAAA
    TTTGTCACAC AGTGGAGGCG ACCTTTAGCT TCAAGGGCCA AAGATATAGG AATTTGGTAT
    GGGATTCTTG AAGGCATTGG AATTCTCTCC GTTATTACAA ATGCATTTGT GATAGCGATC
    ACATCTGACT TTATCCCTCG CTTGGTATAT GCTTATAAGT ATGGACCTTG TGCGGGCCAA
    GGAGAAGCTG GGCAAAAGTG CATGGTTGGC TATGTGAATG CCAGCTTGTC TGTATTCCGA
    ATTTCTGACT TTGAGAACAG ATCTGAACCT GAGTCTGATG GCAGTGAGTT CTCGGGGACT
    CCTCTCAAGT ACTGTAGATA CCGAGACTAC CGTGACCCTC CTCATTCACT GGTGCCCTAC
    GGCTACACAC TACAGTTCTG GCACGTCCTA GCAGCCCGAC TGGCTTTTAT CATTGTGTTC
    GAGCACCTCG TGTTTTGTAT AAAGCACCTC ATTTCATATC TGATCCCAGA CCTCCCAAAA
    GATCTAAGGG ATCGAATGAG AAGAGAGAAG TACTTGATTC AGGAGATGAT GTATGAAGCA
    GAACTGGAGC GTCTCCAGAA GGAACGAAAG GAGAGGAAGA AAAATGGAAA AGCACACCAC
    AATGAGTGGC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90751
    Clone ID Related Accession (Same CDS sequence) XM_015471092.1
    Accession Version XM_015471092.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGATCGATAA ACAAATAAGC AGGTTTCGGA GATGGTTACC TAAGAAGCCA
    ATGAGGCTGG ACAAGGAGAC ACTGCCAGAC CTGGAGGAGA ATGACTGCTA CACTGCCCCT
    TTCAGCCAGC AAAGGATCCA TCACTTCATC ATACACAACA AAGACACTTT CTTCAACAAT
    GCCACAAGAA GTAGAATTGT ACATCATATT TTACAAAGAA TAAAGTATGA AGAAGGAAAA
    AACAAAATTG GTCTGAATCG TTTGCTTACT AATGGCTCCT ATGAAGCTGC ATTTCCTCTG
    CACGAGGGAA GTTACAGAAG TAAAAACTCC ATCAGAACCC ACGGAGCGGT AAACCACCGA
    CATCTGCTCT ATGAGTGCTG GGCGTCCTGG GGTGTGTGGT ACAAATACCA GCCTTTGGAT
    CTTGTAAGGC GGTACTTTGG AGAGAAGATT GGGTTGTACT TTGCCTGGTT GGGCTGGTAC
    ACCGGCATGC TCTTCCCAGC TGCCTTCATT GGATTGTTTG TCTTTTTGTA TGGCGTCATC
    ACTCTGGATC ACTGCCAAGT CAGTAAAGAA GTCTGCCAAG CTACAGATAT CATCATGTGT
    CCTGTGTGTG ATAAATACTG TCCATTCATG AGGCTGTCAG ACAGCTGCGT TTATGCCAAG
    GTAACCCATC TTTTTGACAA TGGAGCCACT GTTTTCTTTG CTGTTTTCAT GGCAGTCTGG
    GCAACAGTTT TCCTAGAGTT TTGGAAGAGA CGGAGAGCAG TAATTGCTTA TGACTGGGAT
    TTGATAGACT GGGAAGAAGA GGAGGAAGAA ATACGGCCCC AGTTTGAAGC CAAGTATTCC
    AAGAAAGAGA GGATGAATCC CATTTCCGGC AAGCCAGAAC CATATCAAGC ATTTGCAGAT
    AAATGCAGCC GACTTATTGT TTCTGCATCT GGAATATTTT TTATGATCTG CGTGGTGATC
    GCCGCTGTGT TCGGGATTGT CATTTACAGG GTGGTGACCG TCAGCACTTT CGCAGCCTTT
    AAGTGGGCAT TAATCAGGAA TAATTCTCAG GTTGCAACTA CAGGAACTGC TGTGTGCATC
    AATTTCTGCA TCATTATGCT GCTGAATGTG CTCTATGAAA AGGTTGCTCT TCTTCTGACA
    AATTTAGAAC AGCCTCGCAC AGAGTCCGAG TGGGAGAACA GCTTCACCCT GAAAATGTTT
    CTTTTTCAGT TTGTCAATCT GAACAGCTCC ACATTTTACA TCGCGTTCTT CCTCGGAAGA
    TTCACAGGAC ACCCAGGTGC CTACTTGAGG CTGATAAACA GGTGGAGACT AGAAGAGTGC
    CACCCTAGTG GATGCCTTAT TGATCTCTGT ATGCAAATGG GTATTATAAT GGTGCTAAAG
    CAGACCTGGA ATAATTTCAT GGAACTTGGC TACCCGTTAA TTCAGAATTG GTGGACTAGA
    AGAAAAGTAC GACAAGAACA TGGGCCTGAA AGGAAAATAA GTTTCCCACA ATGGGAAAAG
    GACTACAACC TTCAGCCGAT GAATGCCTAC GGACTCTTTG ATGAATACTT AGAAATGATT
    CTTCAGTTTG GATTTACAAC TATCTTTGTG GCAGCTTTTC CCCTAGCACC ACTTCTGGCC
    TTACTGAATA ATATAATTGA AATTCGACTT GACGCTTATA AATTTGTCAC ACAGTGGAGG
    CGACCTTTAG CTTCAAGGGC CAAAGATATA GGAATTTGGT ATGGGATTCT TGAAGGCATT
    GGAATTCTCT CCGTTATTAC AAATGCATTT GTGATAGCGA TCACATCTGA CTTTATCCCT
    CGCTTGGTAT ATGCTTATAA GTATGGACCT TGTGCGGGCC AAGGAGAAGC TGGGCAAAAG
    TGCATGGTTG GCTATGTGAA TGCCAGCTTG TCTGTATTCC GAATTTCTGA CTTTGAGAAC
    AGATCTGAAC CTGAGTCTGA TGGCAGTGAG TTCTCGGGGA CTCCTCTCAA GTACTGTAGA
    TACCGAGACT ACCGTGACCC TCCTCATTCA CTGGTGCCCT ACGGCTACAC ACTACAGTTC
    TGGCACGTCC TAGCAGCCCG ACTGGCTTTT ATCATTGTGT TCGAGCACCT CGTGTTTTGT
    ATAAAGCACC TCATTTCATA TCTGATCCCA GACCTCCCAA AAGATCTAAG GGATCGAATG
    AGAAGAGAGA AGTACTTGAT TCAGGAGATG ATGTATGAAG CAGAACTGGA GCGTCTCCAG
    AAGGAACGAA AGGAGAGGAA GAAAAATGGA AAAGCACACC ACAATGAGTG GCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326578.1
    CDS371..3247
    Translation

    Target ORF information:

    RefSeq Version XM_015471092.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471092.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GGATCGATAA ACAAATAAGC AGGTTTCGGA GATGGTTACC TAAGAAGCCA
    ATGAGGCTGG ACAAGGAGAC ACTGCCAGAC CTGGAGGAGA ATGACTGCTA CACTGCCCCT
    TTCAGCCAGC AAAGGATCCA TCACTTCATC ATACACAACA AAGACACTTT CTTCAACAAT
    GCCACAAGAA GTAGAATTGT ACATCATATT TTACAAAGAA TAAAGTATGA AGAAGGAAAA
    AACAAAATTG GTCTGAATCG TTTGCTTACT AATGGCTCCT ATGAAGCTGC ATTTCCTCTG
    CACGAGGGAA GTTACAGAAG TAAAAACTCC ATCAGAACCC ACGGAGCGGT AAACCACCGA
    CATCTGCTCT ATGAGTGCTG GGCGTCCTGG GGTGTGTGGT ACAAATACCA GCCTTTGGAT
    CTTGTAAGGC GGTACTTTGG AGAGAAGATT GGGTTGTACT TTGCCTGGTT GGGCTGGTAC
    ACCGGCATGC TCTTCCCAGC TGCCTTCATT GGATTGTTTG TCTTTTTGTA TGGCGTCATC
    ACTCTGGATC ACTGCCAAGT CAGTAAAGAA GTCTGCCAAG CTACAGATAT CATCATGTGT
    CCTGTGTGTG ATAAATACTG TCCATTCATG AGGCTGTCAG ACAGCTGCGT TTATGCCAAG
    GTAACCCATC TTTTTGACAA TGGAGCCACT GTTTTCTTTG CTGTTTTCAT GGCAGTCTGG
    GCAACAGTTT TCCTAGAGTT TTGGAAGAGA CGGAGAGCAG TAATTGCTTA TGACTGGGAT
    TTGATAGACT GGGAAGAAGA GGAGGAAGAA ATACGGCCCC AGTTTGAAGC CAAGTATTCC
    AAGAAAGAGA GGATGAATCC CATTTCCGGC AAGCCAGAAC CATATCAAGC ATTTGCAGAT
    AAATGCAGCC GACTTATTGT TTCTGCATCT GGAATATTTT TTATGATCTG CGTGGTGATC
    GCCGCTGTGT TCGGGATTGT CATTTACAGG GTGGTGACCG TCAGCACTTT CGCAGCCTTT
    AAGTGGGCAT TAATCAGGAA TAATTCTCAG GTTGCAACTA CAGGAACTGC TGTGTGCATC
    AATTTCTGCA TCATTATGCT GCTGAATGTG CTCTATGAAA AGGTTGCTCT TCTTCTGACA
    AATTTAGAAC AGCCTCGCAC AGAGTCCGAG TGGGAGAACA GCTTCACCCT GAAAATGTTT
    CTTTTTCAGT TTGTCAATCT GAACAGCTCC ACATTTTACA TCGCGTTCTT CCTCGGAAGA
    TTCACAGGAC ACCCAGGTGC CTACTTGAGG CTGATAAACA GGTGGAGACT AGAAGAGTGC
    CACCCTAGTG GATGCCTTAT TGATCTCTGT ATGCAAATGG GTATTATAAT GGTGCTAAAG
    CAGACCTGGA ATAATTTCAT GGAACTTGGC TACCCGTTAA TTCAGAATTG GTGGACTAGA
    AGAAAAGTAC GACAAGAACA TGGGCCTGAA AGGAAAATAA GTTTCCCACA ATGGGAAAAG
    GACTACAACC TTCAGCCGAT GAATGCCTAC GGACTCTTTG ATGAATACTT AGAAATGATT
    CTTCAGTTTG GATTTACAAC TATCTTTGTG GCAGCTTTTC CCCTAGCACC ACTTCTGGCC
    TTACTGAATA ATATAATTGA AATTCGACTT GACGCTTATA AATTTGTCAC ACAGTGGAGG
    CGACCTTTAG CTTCAAGGGC CAAAGATATA GGAATTTGGT ATGGGATTCT TGAAGGCATT
    GGAATTCTCT CCGTTATTAC AAATGCATTT GTGATAGCGA TCACATCTGA CTTTATCCCT
    CGCTTGGTAT ATGCTTATAA GTATGGACCT TGTGCGGGCC AAGGAGAAGC TGGGCAAAAG
    TGCATGGTTG GCTATGTGAA TGCCAGCTTG TCTGTATTCC GAATTTCTGA CTTTGAGAAC
    AGATCTGAAC CTGAGTCTGA TGGCAGTGAG TTCTCGGGGA CTCCTCTCAA GTACTGTAGA
    TACCGAGACT ACCGTGACCC TCCTCATTCA CTGGTGCCCT ACGGCTACAC ACTACAGTTC
    TGGCACGTCC TAGCAGCCCG ACTGGCTTTT ATCATTGTGT TCGAGCACCT CGTGTTTTGT
    ATAAAGCACC TCATTTCATA TCTGATCCCA GACCTCCCAA AAGATCTAAG GGATCGAATG
    AGAAGAGAGA AGTACTTGAT TCAGGAGATG ATGTATGAAG CAGAACTGGA GCGTCTCCAG
    AAGGAACGAA AGGAGAGGAA GAAAAATGGA AAAGCACACC ACAATGAGTG GCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90752
    Clone ID Related Accession (Same CDS sequence) XM_015471093.1
    Accession Version XM_015471093.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GCTTCATCAT ACACAACAAA GACACTTTCT TCAACAATGC CACAAGAAGT
    AGAATTGTAC ATCATATTTT ACAAAGAATA AAGTATGAAG AAGGAAAAAA CAAAATTGGT
    CTGAATCGTT TGCTTACTAA TGGCTCCTAT GAAGCTGCAT TTCCTCTGCA CGAGGGAAGT
    TACAGAAGTA AAAACTCCAT CAGAACCCAC GGAGCGGTAA ACCACCGACA TCTGCTCTAT
    GAGTGCTGGG CGTCCTGGGG TGTGTGGTAC AAATACCAGC CTTTGGATCT TGTAAGGCGG
    TACTTTGGAG AGAAGATTGG GTTGTACTTT GCCTGGTTGG GCTGGTACAC CGGCATGCTC
    TTCCCAGCTG CCTTCATTGG ATTGTTTGTC TTTTTGTATG GCGTCATCAC TCTGGATCAC
    TGCCAAGTCA GTAAAGAAGT CTGCCAAGCT ACAGATATCA TCATGTGTCC TGTGTGTGAT
    AAATACTGTC CATTCATGAG GCTGTCAGAC AGCTGCGTTT ATGCCAAGGT AACCCATCTT
    TTTGACAATG GAGCCACTGT TTTCTTTGCT GTTTTCATGG CAGTCTGGGC AACAGTTTTC
    CTAGAGTTTT GGAAGAGACG GAGAGCAGTA ATTGCTTATG ACTGGGATTT GATAGACTGG
    GAAGAAGAGG AGGAAGAAAT ACGGCCCCAG TTTGAAGCCA AGTATTCCAA GAAAGAGAGG
    ATGAATCCCA TTTCCGGCAA GCCAGAACCA TATCAAGCAT TTGCAGATAA ATGCAGCCGA
    CTTATTGTTT CTGCATCTGG AATATTTTTT ATGATCTGCG TGGTGATCGC CGCTGTGTTC
    GGGATTGTCA TTTACAGGGT GGTGACCGTC AGCACTTTCG CAGCCTTTAA GTGGGCATTA
    ATCAGGAATA ATTCTCAGGT TGCAACTACA GGAACTGCTG TGTGCATCAA TTTCTGCATC
    ATTATGCTGC TGAATGTGCT CTATGAAAAG GTTGCTCTTC TTCTGACAAA TTTAGAACAG
    CCTCGCACAG AGTCCGAGTG GGAGAACAGC TTCACCCTGA AAATGTTTCT TTTTCAGTTT
    GTCAATCTGA ACAGCTCCAC ATTTTACATC GCGTTCTTCC TCGGAAGATT CACAGGACAC
    CCAGGTGCCT ACTTGAGGCT GATAAACAGG TGGAGACTAG AAGAGTGCCA CCCTAGTGGA
    TGCCTTATTG ATCTCTGTAT GCAAATGGGT ATTATAATGG TGCTAAAGCA GACCTGGAAT
    AATTTCATGG AACTTGGCTA CCCGTTAATT CAGAATTGGT GGACTAGAAG AAAAGTACGA
    CAAGAACATG GGCCTGAAAG GAAAATAAGT TTCCCACAAT GGGAAAAGGA CTACAACCTT
    CAGCCGATGA ATGCCTACGG ACTCTTTGAT GAATACTTAG AAATGATTCT TCAGTTTGGA
    TTTACAACTA TCTTTGTGGC AGCTTTTCCC CTAGCACCAC TTCTGGCCTT ACTGAATAAT
    ATAATTGAAA TTCGACTTGA CGCTTATAAA TTTGTCACAC AGTGGAGGCG ACCTTTAGCT
    TCAAGGGCCA AAGATATAGG AATTTGGTAT GGGATTCTTG AAGGCATTGG AATTCTCTCC
    GTTATTACAA ATGCATTTGT GATAGCGATC ACATCTGACT TTATCCCTCG CTTGGTATAT
    GCTTATAAGT ATGGACCTTG TGCGGGCCAA GGAGAAGCTG GGCAAAAGTG CATGGTTGGC
    TATGTGAATG CCAGCTTGTC TGTATTCCGA ATTTCTGACT TTGAGAACAG ATCTGAACCT
    GAGTCTGATG GCAGTGAGTT CTCGGGGACT CCTCTCAAGT ACTGTAGATA CCGAGACTAC
    CGTGACCCTC CTCATTCACT GGTGCCCTAC GGCTACACAC TACAGTTCTG GCACGTCCTA
    GCAGCCCGAC TGGCTTTTAT CATTGTGTTC GAGCACCTCG TGTTTTGTAT AAAGCACCTC
    ATTTCATATC TGATCCCAGA CCTCCCAAAA GATCTAAGGG ATCGAATGAG AAGAGAGAAG
    TACTTGATTC AGGAGATGAT GTATGAAGCA GAACTGGAGC GTCTCCAGAA GGAACGAAAG
    GAGAGGAAGA AAAATGGAAA AGCACACCAC AATGAGTGGC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326579.1
    CDS371..3115
    Translation

    Target ORF information:

    RefSeq Version XM_015471093.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471093.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGGCTGA AAAGCGTTTG CAAATCCACC AACGGCGAAG TGTGGCAAGC CGCCCAGGGT 
    CACGTCGCCT GCCCGTGGTC AGGCATTCCT CACTTCCACC AGGCAGAAGA AGGAGGTGTG
    GATTTGCAAA GCTACCAGCT GGATATGCAA ATACTACCTG ACGGGCCAAG GAGTGATGTG
    GACTTTTCAG AGATTCTTAA TGCAATACAA GAAATGGCCA AGGATGTCAA TATTCTTTTT
    GATGAATTAG AAGCTGTCAA CAGTCCTTGC AAAGATGACG ACTCTCTCCT TCACCCTGGA
    AATCTTACCA GTACTTCAGA TGATGCTAGC AGGTTGGAAG CCGGGGGAGA GACAGTACCA
    GAAAAAAACA AATTAAATGG CCTTTACTTC AGAGACGGAA AATGTCGAAT TGACTACATT
    CTTGTGTACA GAAAATCCAA CCCCCAGATG GAAAAGAGAG AAGTATTTGA AAGAAATATT
    AGAGCAGAAG GATTACAAAT GGAGAAAGAG TCCTCTCTAA TAAATAGTGA CATTATCTTT
    GTGAAGTTGC ATGCCCCATG GGAAGTGCTT GGAAGATATG CAGAACAAAT GAATGTAAGA
    ATGCCTTTCA GCTTCATCAT ACACAACAAA GACACTTTCT TCAACAATGC CACAAGAAGT
    AGAATTGTAC ATCATATTTT ACAAAGAATA AAGTATGAAG AAGGAAAAAA CAAAATTGGT
    CTGAATCGTT TGCTTACTAA TGGCTCCTAT GAAGCTGCAT TTCCTCTGCA CGAGGGAAGT
    TACAGAAGTA AAAACTCCAT CAGAACCCAC GGAGCGGTAA ACCACCGACA TCTGCTCTAT
    GAGTGCTGGG CGTCCTGGGG TGTGTGGTAC AAATACCAGC CTTTGGATCT TGTAAGGCGG
    TACTTTGGAG AGAAGATTGG GTTGTACTTT GCCTGGTTGG GCTGGTACAC CGGCATGCTC
    TTCCCAGCTG CCTTCATTGG ATTGTTTGTC TTTTTGTATG GCGTCATCAC TCTGGATCAC
    TGCCAAGTCA GTAAAGAAGT CTGCCAAGCT ACAGATATCA TCATGTGTCC TGTGTGTGAT
    AAATACTGTC CATTCATGAG GCTGTCAGAC AGCTGCGTTT ATGCCAAGGT AACCCATCTT
    TTTGACAATG GAGCCACTGT TTTCTTTGCT GTTTTCATGG CAGTCTGGGC AACAGTTTTC
    CTAGAGTTTT GGAAGAGACG GAGAGCAGTA ATTGCTTATG ACTGGGATTT GATAGACTGG
    GAAGAAGAGG AGGAAGAAAT ACGGCCCCAG TTTGAAGCCA AGTATTCCAA GAAAGAGAGG
    ATGAATCCCA TTTCCGGCAA GCCAGAACCA TATCAAGCAT TTGCAGATAA ATGCAGCCGA
    CTTATTGTTT CTGCATCTGG AATATTTTTT ATGATCTGCG TGGTGATCGC CGCTGTGTTC
    GGGATTGTCA TTTACAGGGT GGTGACCGTC AGCACTTTCG CAGCCTTTAA GTGGGCATTA
    ATCAGGAATA ATTCTCAGGT TGCAACTACA GGAACTGCTG TGTGCATCAA TTTCTGCATC
    ATTATGCTGC TGAATGTGCT CTATGAAAAG GTTGCTCTTC TTCTGACAAA TTTAGAACAG
    CCTCGCACAG AGTCCGAGTG GGAGAACAGC TTCACCCTGA AAATGTTTCT TTTTCAGTTT
    GTCAATCTGA ACAGCTCCAC ATTTTACATC GCGTTCTTCC TCGGAAGATT CACAGGACAC
    CCAGGTGCCT ACTTGAGGCT GATAAACAGG TGGAGACTAG AAGAGTGCCA CCCTAGTGGA
    TGCCTTATTG ATCTCTGTAT GCAAATGGGT ATTATAATGG TGCTAAAGCA GACCTGGAAT
    AATTTCATGG AACTTGGCTA CCCGTTAATT CAGAATTGGT GGACTAGAAG AAAAGTACGA
    CAAGAACATG GGCCTGAAAG GAAAATAAGT TTCCCACAAT GGGAAAAGGA CTACAACCTT
    CAGCCGATGA ATGCCTACGG ACTCTTTGAT GAATACTTAG AAATGATTCT TCAGTTTGGA
    TTTACAACTA TCTTTGTGGC AGCTTTTCCC CTAGCACCAC TTCTGGCCTT ACTGAATAAT
    ATAATTGAAA TTCGACTTGA CGCTTATAAA TTTGTCACAC AGTGGAGGCG ACCTTTAGCT
    TCAAGGGCCA AAGATATAGG AATTTGGTAT GGGATTCTTG AAGGCATTGG AATTCTCTCC
    GTTATTACAA ATGCATTTGT GATAGCGATC ACATCTGACT TTATCCCTCG CTTGGTATAT
    GCTTATAAGT ATGGACCTTG TGCGGGCCAA GGAGAAGCTG GGCAAAAGTG CATGGTTGGC
    TATGTGAATG CCAGCTTGTC TGTATTCCGA ATTTCTGACT TTGAGAACAG ATCTGAACCT
    GAGTCTGATG GCAGTGAGTT CTCGGGGACT CCTCTCAAGT ACTGTAGATA CCGAGACTAC
    CGTGACCCTC CTCATTCACT GGTGCCCTAC GGCTACACAC TACAGTTCTG GCACGTCCTA
    GCAGCCCGAC TGGCTTTTAT CATTGTGTTC GAGCACCTCG TGTTTTGTAT AAAGCACCTC
    ATTTCATATC TGATCCCAGA CCTCCCAAAA GATCTAAGGG ATCGAATGAG AAGAGAGAAG
    TACTTGATTC AGGAGATGAT GTATGAAGCA GAACTGGAGC GTCTCCAGAA GGAACGAAAG
    GAGAGGAAGA AAAATGGAAA AGCACACCAC AATGAGTGGC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234381
    Clone ID Related Accession (Same CDS sequence) XM_024991739.1 , XM_024991738.1
    Accession Version XM_024991739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGG CGGTACTTTG GAGAGAAGAT TGGGTTGTAC
    TTTGCCTGGT TGGGCTGGTA CACCGGCATG CTCTTCCCAG CTGCCTTCAT TGGATTGTTT
    GTCTTTTTGT ATGGCGTCAT CACTCTGGAT CACTGCCAAG TCAGTAAAGA AGTCTGCCAA
    GCTACAGATA TCATCATGTG TCCTGTGTGT GATAAATACT GTCCATTCAT GAGGCTGTCA
    GACAGCTGCG TTTATGCCAA GGTAACCCAT CTTTTTGACA ATGGAGCCAC TGTTTTCTTT
    GCTGTTTTCA TGGCAGTCTG GGCAACAGTT TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA
    GTAATTGCTT ATGACTGGGA TTTGATAGAC TGGGAAGAAG AGGAGGAAGA AATACGGCCC
    CAGTTTGAAG CCAAGTATTC CAAGAAAGAG AGGATGAATC CCATTTCCGG CAAGCCAGAA
    CCATATCAAG CATTTGCAGA TAAATGCAGC CGACTTATTG TTTCTGCATC TGGAATATTT
    TTTATGATCT GCGTGGTGAT CGCCGCTGTG TTCGGGATTG TCATTTACAG GGTGGTGACC
    GTCAGCACTT TCGCAGCCTT TAAGTGGGCA TTAATCAGGA ATAATTCTCA GGTTGCAACT
    ACAGGAACTG CTGTGTGCAT CAATTTCTGC ATCATTATGC TGCTGAATGT GCTCTATGAA
    AAGGTTGCTC TTCTTCTGAC AAATTTAGAA CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC
    AGCTTCACCC TGAAAATGTT TCTTTTTCAG TTTGTCAATC TGAACAGCTC CACATTTTAC
    ATCGCGTTCT TCCTCGGAAG ATTCACAGGA CACCCAGGTG CCTACTTGAG GCTGATAAAC
    AGGTGGAGAC TAGAAGAGTG CCACCCTAGT GGATGCCTTA TTGATCTCTG TATGCAAATG
    GGTATTATAA TGGTGCTAAA GCAGACCTGG AATAATTTCA TGGAACTTGG CTACCCGTTA
    ATTCAGAATT GGTGGACTAG AAGAAAAGTA CGACAAGAAC ATGGGCCTGA AAGGAAAATA
    AGTTTCCCAC AATGGGAAAA GGACTACAAC CTTCAGCCGA TGAATGCCTA CGGACTCTTT
    GATGAATACT TAGAAATGAT TCTTCAGTTT GGATTTACAA CTATCTTTGT GGCAGCTTTT
    CCCCTAGCAC CACTTCTGGC CTTACTGAAT AATATAATTG AAATTCGACT TGACGCTTAT
    AAATTTGTCA CACAGTGGAG GCGACCTTTA GCTTCAAGGG CCAAAGATAT AGGAATTTGG
    TATGGGATTC TTGAAGGCAT TGGAATTCTC TCCGTTATTA CAAATGCATT TGTGATAGCG
    ATCACATCTG ACTTTATCCC TCGCTTGGTA TATGCTTATA AGTATGGACC TTGTGCGGGC
    CAAGGAGAAG CTGGGCAAAA GTGCATGGTT GGCTATGTGA ATGCCAGCTT GTCTGTATTC
    CGAATTTCTG ACTTTGAGAA CAGATCTGAA CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG
    ACTCCTCTCA AGTACTGTAG ATACCGAGAC TACCGTGACC CTCCTCATTC ACTGGTGCCC
    TACGGCTACA CACTACAGTT CTGGCACGTC CTAGCAGCCC GACTGGCTTT TATCATTGTG
    TTCGAGCACC TCGTGTTTTG TATAAAGCAC CTCATTTCAT ATCTGATCCC AGACCTCCCA
    AAAGATCTAA GGGATCGAAT GAGAAGAGAG AAGTACTTGA TTCAGGAGAT GATGTATGAA
    GCAGAACTGG AGCGTCTCCA GAAGGAACGA AAGGAGAGGA AGAAAAATGG AAAAGCACAC
    CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847507.1
    CDS168..4085
    Translation

    Target ORF information:

    RefSeq Version XM_024991739.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGG CGGTACTTTG GAGAGAAGAT TGGGTTGTAC
    TTTGCCTGGT TGGGCTGGTA CACCGGCATG CTCTTCCCAG CTGCCTTCAT TGGATTGTTT
    GTCTTTTTGT ATGGCGTCAT CACTCTGGAT CACTGCCAAG TCAGTAAAGA AGTCTGCCAA
    GCTACAGATA TCATCATGTG TCCTGTGTGT GATAAATACT GTCCATTCAT GAGGCTGTCA
    GACAGCTGCG TTTATGCCAA GGTAACCCAT CTTTTTGACA ATGGAGCCAC TGTTTTCTTT
    GCTGTTTTCA TGGCAGTCTG GGCAACAGTT TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA
    GTAATTGCTT ATGACTGGGA TTTGATAGAC TGGGAAGAAG AGGAGGAAGA AATACGGCCC
    CAGTTTGAAG CCAAGTATTC CAAGAAAGAG AGGATGAATC CCATTTCCGG CAAGCCAGAA
    CCATATCAAG CATTTGCAGA TAAATGCAGC CGACTTATTG TTTCTGCATC TGGAATATTT
    TTTATGATCT GCGTGGTGAT CGCCGCTGTG TTCGGGATTG TCATTTACAG GGTGGTGACC
    GTCAGCACTT TCGCAGCCTT TAAGTGGGCA TTAATCAGGA ATAATTCTCA GGTTGCAACT
    ACAGGAACTG CTGTGTGCAT CAATTTCTGC ATCATTATGC TGCTGAATGT GCTCTATGAA
    AAGGTTGCTC TTCTTCTGAC AAATTTAGAA CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC
    AGCTTCACCC TGAAAATGTT TCTTTTTCAG TTTGTCAATC TGAACAGCTC CACATTTTAC
    ATCGCGTTCT TCCTCGGAAG ATTCACAGGA CACCCAGGTG CCTACTTGAG GCTGATAAAC
    AGGTGGAGAC TAGAAGAGTG CCACCCTAGT GGATGCCTTA TTGATCTCTG TATGCAAATG
    GGTATTATAA TGGTGCTAAA GCAGACCTGG AATAATTTCA TGGAACTTGG CTACCCGTTA
    ATTCAGAATT GGTGGACTAG AAGAAAAGTA CGACAAGAAC ATGGGCCTGA AAGGAAAATA
    AGTTTCCCAC AATGGGAAAA GGACTACAAC CTTCAGCCGA TGAATGCCTA CGGACTCTTT
    GATGAATACT TAGAAATGAT TCTTCAGTTT GGATTTACAA CTATCTTTGT GGCAGCTTTT
    CCCCTAGCAC CACTTCTGGC CTTACTGAAT AATATAATTG AAATTCGACT TGACGCTTAT
    AAATTTGTCA CACAGTGGAG GCGACCTTTA GCTTCAAGGG CCAAAGATAT AGGAATTTGG
    TATGGGATTC TTGAAGGCAT TGGAATTCTC TCCGTTATTA CAAATGCATT TGTGATAGCG
    ATCACATCTG ACTTTATCCC TCGCTTGGTA TATGCTTATA AGTATGGACC TTGTGCGGGC
    CAAGGAGAAG CTGGGCAAAA GTGCATGGTT GGCTATGTGA ATGCCAGCTT GTCTGTATTC
    CGAATTTCTG ACTTTGAGAA CAGATCTGAA CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG
    ACTCCTCTCA AGTACTGTAG ATACCGAGAC TACCGTGACC CTCCTCATTC ACTGGTGCCC
    TACGGCTACA CACTACAGTT CTGGCACGTC CTAGCAGCCC GACTGGCTTT TATCATTGTG
    TTCGAGCACC TCGTGTTTTG TATAAAGCAC CTCATTTCAT ATCTGATCCC AGACCTCCCA
    AAAGATCTAA GGGATCGAAT GAGAAGAGAG AAGTACTTGA TTCAGGAGAT GATGTATGAA
    GCAGAACTGG AGCGTCTCCA GAAGGAACGA AAGGAGAGGA AGAAAAATGG AAAAGCACAC
    CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234381
    Clone ID Related Accession (Same CDS sequence) XM_024991739.1 , XM_024991738.1
    Accession Version XM_024991738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGG CGGTACTTTG GAGAGAAGAT TGGGTTGTAC
    TTTGCCTGGT TGGGCTGGTA CACCGGCATG CTCTTCCCAG CTGCCTTCAT TGGATTGTTT
    GTCTTTTTGT ATGGCGTCAT CACTCTGGAT CACTGCCAAG TCAGTAAAGA AGTCTGCCAA
    GCTACAGATA TCATCATGTG TCCTGTGTGT GATAAATACT GTCCATTCAT GAGGCTGTCA
    GACAGCTGCG TTTATGCCAA GGTAACCCAT CTTTTTGACA ATGGAGCCAC TGTTTTCTTT
    GCTGTTTTCA TGGCAGTCTG GGCAACAGTT TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA
    GTAATTGCTT ATGACTGGGA TTTGATAGAC TGGGAAGAAG AGGAGGAAGA AATACGGCCC
    CAGTTTGAAG CCAAGTATTC CAAGAAAGAG AGGATGAATC CCATTTCCGG CAAGCCAGAA
    CCATATCAAG CATTTGCAGA TAAATGCAGC CGACTTATTG TTTCTGCATC TGGAATATTT
    TTTATGATCT GCGTGGTGAT CGCCGCTGTG TTCGGGATTG TCATTTACAG GGTGGTGACC
    GTCAGCACTT TCGCAGCCTT TAAGTGGGCA TTAATCAGGA ATAATTCTCA GGTTGCAACT
    ACAGGAACTG CTGTGTGCAT CAATTTCTGC ATCATTATGC TGCTGAATGT GCTCTATGAA
    AAGGTTGCTC TTCTTCTGAC AAATTTAGAA CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC
    AGCTTCACCC TGAAAATGTT TCTTTTTCAG TTTGTCAATC TGAACAGCTC CACATTTTAC
    ATCGCGTTCT TCCTCGGAAG ATTCACAGGA CACCCAGGTG CCTACTTGAG GCTGATAAAC
    AGGTGGAGAC TAGAAGAGTG CCACCCTAGT GGATGCCTTA TTGATCTCTG TATGCAAATG
    GGTATTATAA TGGTGCTAAA GCAGACCTGG AATAATTTCA TGGAACTTGG CTACCCGTTA
    ATTCAGAATT GGTGGACTAG AAGAAAAGTA CGACAAGAAC ATGGGCCTGA AAGGAAAATA
    AGTTTCCCAC AATGGGAAAA GGACTACAAC CTTCAGCCGA TGAATGCCTA CGGACTCTTT
    GATGAATACT TAGAAATGAT TCTTCAGTTT GGATTTACAA CTATCTTTGT GGCAGCTTTT
    CCCCTAGCAC CACTTCTGGC CTTACTGAAT AATATAATTG AAATTCGACT TGACGCTTAT
    AAATTTGTCA CACAGTGGAG GCGACCTTTA GCTTCAAGGG CCAAAGATAT AGGAATTTGG
    TATGGGATTC TTGAAGGCAT TGGAATTCTC TCCGTTATTA CAAATGCATT TGTGATAGCG
    ATCACATCTG ACTTTATCCC TCGCTTGGTA TATGCTTATA AGTATGGACC TTGTGCGGGC
    CAAGGAGAAG CTGGGCAAAA GTGCATGGTT GGCTATGTGA ATGCCAGCTT GTCTGTATTC
    CGAATTTCTG ACTTTGAGAA CAGATCTGAA CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG
    ACTCCTCTCA AGTACTGTAG ATACCGAGAC TACCGTGACC CTCCTCATTC ACTGGTGCCC
    TACGGCTACA CACTACAGTT CTGGCACGTC CTAGCAGCCC GACTGGCTTT TATCATTGTG
    TTCGAGCACC TCGTGTTTTG TATAAAGCAC CTCATTTCAT ATCTGATCCC AGACCTCCCA
    AAAGATCTAA GGGATCGAAT GAGAAGAGAG AAGTACTTGA TTCAGGAGAT GATGTATGAA
    GCAGAACTGG AGCGTCTCCA GAAGGAACGA AAGGAGAGGA AGAAAAATGG AAAAGCACAC
    CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847506.1
    CDS168..4085
    Translation

    Target ORF information:

    RefSeq Version XM_024991738.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGG CGGTACTTTG GAGAGAAGAT TGGGTTGTAC
    TTTGCCTGGT TGGGCTGGTA CACCGGCATG CTCTTCCCAG CTGCCTTCAT TGGATTGTTT
    GTCTTTTTGT ATGGCGTCAT CACTCTGGAT CACTGCCAAG TCAGTAAAGA AGTCTGCCAA
    GCTACAGATA TCATCATGTG TCCTGTGTGT GATAAATACT GTCCATTCAT GAGGCTGTCA
    GACAGCTGCG TTTATGCCAA GGTAACCCAT CTTTTTGACA ATGGAGCCAC TGTTTTCTTT
    GCTGTTTTCA TGGCAGTCTG GGCAACAGTT TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA
    GTAATTGCTT ATGACTGGGA TTTGATAGAC TGGGAAGAAG AGGAGGAAGA AATACGGCCC
    CAGTTTGAAG CCAAGTATTC CAAGAAAGAG AGGATGAATC CCATTTCCGG CAAGCCAGAA
    CCATATCAAG CATTTGCAGA TAAATGCAGC CGACTTATTG TTTCTGCATC TGGAATATTT
    TTTATGATCT GCGTGGTGAT CGCCGCTGTG TTCGGGATTG TCATTTACAG GGTGGTGACC
    GTCAGCACTT TCGCAGCCTT TAAGTGGGCA TTAATCAGGA ATAATTCTCA GGTTGCAACT
    ACAGGAACTG CTGTGTGCAT CAATTTCTGC ATCATTATGC TGCTGAATGT GCTCTATGAA
    AAGGTTGCTC TTCTTCTGAC AAATTTAGAA CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC
    AGCTTCACCC TGAAAATGTT TCTTTTTCAG TTTGTCAATC TGAACAGCTC CACATTTTAC
    ATCGCGTTCT TCCTCGGAAG ATTCACAGGA CACCCAGGTG CCTACTTGAG GCTGATAAAC
    AGGTGGAGAC TAGAAGAGTG CCACCCTAGT GGATGCCTTA TTGATCTCTG TATGCAAATG
    GGTATTATAA TGGTGCTAAA GCAGACCTGG AATAATTTCA TGGAACTTGG CTACCCGTTA
    ATTCAGAATT GGTGGACTAG AAGAAAAGTA CGACAAGAAC ATGGGCCTGA AAGGAAAATA
    AGTTTCCCAC AATGGGAAAA GGACTACAAC CTTCAGCCGA TGAATGCCTA CGGACTCTTT
    GATGAATACT TAGAAATGAT TCTTCAGTTT GGATTTACAA CTATCTTTGT GGCAGCTTTT
    CCCCTAGCAC CACTTCTGGC CTTACTGAAT AATATAATTG AAATTCGACT TGACGCTTAT
    AAATTTGTCA CACAGTGGAG GCGACCTTTA GCTTCAAGGG CCAAAGATAT AGGAATTTGG
    TATGGGATTC TTGAAGGCAT TGGAATTCTC TCCGTTATTA CAAATGCATT TGTGATAGCG
    ATCACATCTG ACTTTATCCC TCGCTTGGTA TATGCTTATA AGTATGGACC TTGTGCGGGC
    CAAGGAGAAG CTGGGCAAAA GTGCATGGTT GGCTATGTGA ATGCCAGCTT GTCTGTATTC
    CGAATTTCTG ACTTTGAGAA CAGATCTGAA CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG
    ACTCCTCTCA AGTACTGTAG ATACCGAGAC TACCGTGACC CTCCTCATTC ACTGGTGCCC
    TACGGCTACA CACTACAGTT CTGGCACGTC CTAGCAGCCC GACTGGCTTT TATCATTGTG
    TTCGAGCACC TCGTGTTTTG TATAAAGCAC CTCATTTCAT ATCTGATCCC AGACCTCCCA
    AAAGATCTAA GGGATCGAAT GAGAAGAGAG AAGTACTTGA TTCAGGAGAT GATGTATGAA
    GCAGAACTGG AGCGTCTCCA GAAGGAACGA AAGGAGAGGA AGAAAAATGG AAAAGCACAC
    CACAATGAGT GGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234382
    Clone ID Related Accession (Same CDS sequence) XM_024991740.1
    Accession Version XM_024991740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAT CGATAAACAA
    ATAAGCAGGT TTCGGAGATG GTTACCTAAG AAGCCAATGA GGCTGGACAA GGAGACACTG
    CCAGACCTGG AGGAGAATGA CTGCTACACT GCCCCTTTCA GCCAGCAAAG GATCCATCAC
    TTCATCATAC ACAACAAAGA CACTTTCTTC AACAATGCCA CAAGAAGTAG AATTGTACAT
    CATATTTTAC AAAGAATAAA GTATGAAGAA GGAAAAAACA AAATTGGTCT GAATCGTTTG
    CTTACTAATG GCTCCTATGA AGCTGCATTT CCTCTGCACG AGGGAAGTTA CAGAAGTAAA
    AACTCCATCA GAACCCACGG AGCGGTAAAC CACCGACATC TGCTCTATGA GTGCTGGGCG
    TCCTGGGGTG TGTGGTACAA ATACCAGCCT TTGGATCTTG TAAGGCGGTA CTTTGGAGAG
    AAGATTGGGT TGTACTTTGC CTGGTTGGGC TGGTACACCG GCATGCTCTT CCCAGCTGCC
    TTCATTGGAT TGTTTGTCTT TTTGTATGGC GTCATCACTC TGGATCACTG CCAAGTCAGT
    AAAGAAGTCT GCCAAGCTAC AGATATCATC ATGTGTCCTG TGTGTGATAA ATACTGTCCA
    TTCATGAGGC TGTCAGACAG CTGCGTTTAT GCCAAGGTAA CCCATCTTTT TGACAATGGA
    GCCACTGTTT TCTTTGCTGT TTTCATGGCA GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG
    AAGAGACGGA GAGCAGTAAT TGCTTATGAC TGGGATTTGA TAGACTGGGA AGAAGAGGAG
    GAAGAAATAC GGCCCCAGTT TGAAGCCAAG TATTCCAAGA AAGAGAGGAT GAATCCCATT
    TCCGGCAAGC CAGAACCATA TCAAGCATTT GCAGATAAAT GCAGCCGACT TATTGTTTCT
    GCATCTGGAA TATTTTTTAT GATCTGCGTG GTGATCGCCG CTGTGTTCGG GATTGTCATT
    TACAGGGTGG TGACCGTCAG CACTTTCGCA GCCTTTAAGT GGGCATTAAT CAGGAATAAT
    TCTCAGGTTG CAACTACAGG AACTGCTGTG TGCATCAATT TCTGCATCAT TATGCTGCTG
    AATGTGCTCT ATGAAAAGGT TGCTCTTCTT CTGACAAATT TAGAACAGCC TCGCACAGAG
    TCCGAGTGGG AGAACAGCTT CACCCTGAAA ATGTTTCTTT TTCAGTTTGT CAATCTGAAC
    AGCTCCACAT TTTACATCGC GTTCTTCCTC GGAAGATTCA CAGGACACCC AGGTGCCTAC
    TTGAGGCTGA TAAACAGGTG GAGACTAGAA GAGTGCCACC CTAGTGGATG CCTTATTGAT
    CTCTGTATGC AAATGGGTAT TATAATGGTG CTAAAGCAGA CCTGGAATAA TTTCATGGAA
    CTTGGCTACC CGTTAATTCA GAATTGGTGG ACTAGAAGAA AAGTACGACA AGAACATGGG
    CCTGAAAGGA AAATAAGTTT CCCACAATGG GAAAAGGACT ACAACCTTCA GCCGATGAAT
    GCCTACGGAC TCTTTGATGA ATACTTAGAA ATGATTCTTC AGTTTGGATT TACAACTATC
    TTTGTGGCAG CTTTTCCCCT AGCACCACTT CTGGCCTTAC TGAATAATAT AATTGAAATT
    CGACTTGACG CTTATAAATT TGTCACACAG TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA
    GATATAGGAA TTTGGTATGG GATTCTTGAA GGCATTGGAA TTCTCTCCGT TATTACAAAT
    GCATTTGTGA TAGCGATCAC ATCTGACTTT ATCCCTCGCT TGGTATATGC TTATAAGTAT
    GGACCTTGTG CGGGCCAAGG AGAAGCTGGG CAAAAGTGCA TGGTTGGCTA TGTGAATGCC
    AGCTTGTCTG TATTCCGAAT TTCTGACTTT GAGAACAGAT CTGAACCTGA GTCTGATGGC
    AGTGAGTTCT CGGGGACTCC TCTCAAGTAC TGTAGATACC GAGACTACCG TGACCCTCCT
    CATTCACTGG TGCCCTACGG CTACACACTA CAGTTCTGGC ACGTCCTAGC AGCCCGACTG
    GCTTTTATCA TTGTGTTCGA GCACCTCGTG TTTTGTATAA AGCACCTCAT TTCATATCTG
    ATCCCAGACC TCCCAAAAGA TCTAAGGGAT CGAATGAGAA GAGAGAAGTA CTTGATTCAG
    GAGATGATGT ATGAAGCAGA ACTGGAGCGT CTCCAGAAGG AACGAAAGGA GAGGAAGAAA
    AATGGAAAAG CACACCACAA TGAGTGGCCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847508.1
    CDS167..4039
    Translation

    Target ORF information:

    RefSeq Version XM_024991740.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAT CGATAAACAA
    ATAAGCAGGT TTCGGAGATG GTTACCTAAG AAGCCAATGA GGCTGGACAA GGAGACACTG
    CCAGACCTGG AGGAGAATGA CTGCTACACT GCCCCTTTCA GCCAGCAAAG GATCCATCAC
    TTCATCATAC ACAACAAAGA CACTTTCTTC AACAATGCCA CAAGAAGTAG AATTGTACAT
    CATATTTTAC AAAGAATAAA GTATGAAGAA GGAAAAAACA AAATTGGTCT GAATCGTTTG
    CTTACTAATG GCTCCTATGA AGCTGCATTT CCTCTGCACG AGGGAAGTTA CAGAAGTAAA
    AACTCCATCA GAACCCACGG AGCGGTAAAC CACCGACATC TGCTCTATGA GTGCTGGGCG
    TCCTGGGGTG TGTGGTACAA ATACCAGCCT TTGGATCTTG TAAGGCGGTA CTTTGGAGAG
    AAGATTGGGT TGTACTTTGC CTGGTTGGGC TGGTACACCG GCATGCTCTT CCCAGCTGCC
    TTCATTGGAT TGTTTGTCTT TTTGTATGGC GTCATCACTC TGGATCACTG CCAAGTCAGT
    AAAGAAGTCT GCCAAGCTAC AGATATCATC ATGTGTCCTG TGTGTGATAA ATACTGTCCA
    TTCATGAGGC TGTCAGACAG CTGCGTTTAT GCCAAGGTAA CCCATCTTTT TGACAATGGA
    GCCACTGTTT TCTTTGCTGT TTTCATGGCA GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG
    AAGAGACGGA GAGCAGTAAT TGCTTATGAC TGGGATTTGA TAGACTGGGA AGAAGAGGAG
    GAAGAAATAC GGCCCCAGTT TGAAGCCAAG TATTCCAAGA AAGAGAGGAT GAATCCCATT
    TCCGGCAAGC CAGAACCATA TCAAGCATTT GCAGATAAAT GCAGCCGACT TATTGTTTCT
    GCATCTGGAA TATTTTTTAT GATCTGCGTG GTGATCGCCG CTGTGTTCGG GATTGTCATT
    TACAGGGTGG TGACCGTCAG CACTTTCGCA GCCTTTAAGT GGGCATTAAT CAGGAATAAT
    TCTCAGGTTG CAACTACAGG AACTGCTGTG TGCATCAATT TCTGCATCAT TATGCTGCTG
    AATGTGCTCT ATGAAAAGGT TGCTCTTCTT CTGACAAATT TAGAACAGCC TCGCACAGAG
    TCCGAGTGGG AGAACAGCTT CACCCTGAAA ATGTTTCTTT TTCAGTTTGT CAATCTGAAC
    AGCTCCACAT TTTACATCGC GTTCTTCCTC GGAAGATTCA CAGGACACCC AGGTGCCTAC
    TTGAGGCTGA TAAACAGGTG GAGACTAGAA GAGTGCCACC CTAGTGGATG CCTTATTGAT
    CTCTGTATGC AAATGGGTAT TATAATGGTG CTAAAGCAGA CCTGGAATAA TTTCATGGAA
    CTTGGCTACC CGTTAATTCA GAATTGGTGG ACTAGAAGAA AAGTACGACA AGAACATGGG
    CCTGAAAGGA AAATAAGTTT CCCACAATGG GAAAAGGACT ACAACCTTCA GCCGATGAAT
    GCCTACGGAC TCTTTGATGA ATACTTAGAA ATGATTCTTC AGTTTGGATT TACAACTATC
    TTTGTGGCAG CTTTTCCCCT AGCACCACTT CTGGCCTTAC TGAATAATAT AATTGAAATT
    CGACTTGACG CTTATAAATT TGTCACACAG TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA
    GATATAGGAA TTTGGTATGG GATTCTTGAA GGCATTGGAA TTCTCTCCGT TATTACAAAT
    GCATTTGTGA TAGCGATCAC ATCTGACTTT ATCCCTCGCT TGGTATATGC TTATAAGTAT
    GGACCTTGTG CGGGCCAAGG AGAAGCTGGG CAAAAGTGCA TGGTTGGCTA TGTGAATGCC
    AGCTTGTCTG TATTCCGAAT TTCTGACTTT GAGAACAGAT CTGAACCTGA GTCTGATGGC
    AGTGAGTTCT CGGGGACTCC TCTCAAGTAC TGTAGATACC GAGACTACCG TGACCCTCCT
    CATTCACTGG TGCCCTACGG CTACACACTA CAGTTCTGGC ACGTCCTAGC AGCCCGACTG
    GCTTTTATCA TTGTGTTCGA GCACCTCGTG TTTTGTATAA AGCACCTCAT TTCATATCTG
    ATCCCAGACC TCCCAAAAGA TCTAAGGGAT CGAATGAGAA GAGAGAAGTA CTTGATTCAG
    GAGATGATGT ATGAAGCAGA ACTGGAGCGT CTCCAGAAGG AACGAAAGGA GAGGAAGAAA
    AATGGAAAAG CACACCACAA TGAGTGGCCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234383
    Clone ID Related Accession (Same CDS sequence) XM_024991741.1
    Accession Version XM_024991741.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGTGGCC AAGGATGTCA ATATTCTTTT TGATGAATTA
    GAAGCTGTCA ACAGTCCTTG CAAAGATGAC GACTCTCTCC TTCACCCTGG AAATCTTACC
    AGTACTTCAG ATGATGCTAG CAGGTTGGAA GCCGGGGGAG AGACAGTACC AGAAAAAAAC
    AAATTAAATG GCCTTTACTT CAGAGACGGA AAATGTCGAA TTGACTACAT TCTTGTGTAC
    AGAAAATCCA ACCCCCAGAT GGAAAAGAGA GAAGTATTTG AAAGAAATAT TAGAGCAGAA
    GGATTACAAA TGGAGAAAGA GTCCTCTCTA ATAAATAGTG ACATTATCTT TGTGAAGTTG
    CATGCCCCAT GGGAAGTGCT TGGAAGATAT GCAGAACAAA TGAATGTAAG AATGCCTTTC
    AGGAGAAAAA TCTATTACCT GCCCCGCCGG TACAAGTTCA TGAGCAGGAT CGATAAACAA
    ATAAGCAGGT TTCGGAGATG GTTACCTAAG AAGCCAATGA GGCTGGACAA GGAGACACTG
    CCAGACCTGG AGGAGAATGA CTGCTACACT GCCCCTTTCA GCCAGCAAAG GATCCATCAC
    TTCATCATAC ACAACAAAGA CACTTTCTTC AACAATGCCA CAAGAAGTAG AATTGTACAT
    CATATTTTAC AAAGAATAAA GTATGAAGAA GGAAAAAACA AAATTGGTCT GAATCGTTTG
    CTTACTAATG GCTCCTATGA AGCTGCATTT CCTCTGCACG AGGGAAGTTA CAGAAGTAAA
    AACTCCATCA GAACCCACGG AGCGGTAAAC CACCGACATC TGCTCTATGA GTGCTGGGCG
    TCCTGGGGTG TGTGGTACAA ATACCAGCCT TTGGATCTTG TAAGGCGGTA CTTTGGAGAG
    AAGATTGGGT TGTACTTTGC CTGGTTGGGC TGGTACACCG GCATGCTCTT CCCAGCTGCC
    TTCATTGGAT TGTTTGTCTT TTTGTATGGC GTCATCACTC TGGATCACTG CCAAGTCAGT
    AAAGAAGTCT GCCAAGCTAC AGATATCATC ATGTGTCCTG TGTGTGATAA ATACTGTCCA
    TTCATGAGGC TGTCAGACAG CTGCGTTTAT GCCAAGGTAA CCCATCTTTT TGACAATGGA
    GCCACTGTTT TCTTTGCTGT TTTCATGGCA GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG
    AAGAGACGGA GAGCAGTAAT TGCTTATGAC TGGGATTTGA TAGACTGGGA AGAAGAGGAG
    GAAGAAATAC GGCCCCAGTT TGAAGCCAAG TATTCCAAGA AAGAGAGGAT GAATCCCATT
    TCCGGCAAGC CAGAACCATA TCAAGCATTT GCAGATAAAT GCAGCCGACT TATTGTTTCT
    GCATCTGGAA TATTTTTTAT GATCTGCGTG GTGATCGCCG CTGTGTTCGG GATTGTCATT
    TACAGGGTGG TGACCGTCAG CACTTTCGCA GCCTTTAAGT GGGCATTAAT CAGGAATAAT
    TCTCAGGTTG CAACTACAGG AACTGCTGTG TGCATCAATT TCTGCATCAT TATGCTGCTG
    AATGTGCTCT ATGAAAAGGT TGCTCTTCTT CTGACAAATT TAGAACAGCC TCGCACAGAG
    TCCGAGTGGG AGAACAGCTT CACCCTGAAA ATGTTTCTTT TTCAGTTTGT CAATCTGAAC
    AGCTCCACAT TTTACATCGC GTTCTTCCTC GGAAGATTCA CAGGACACCC AGGTGCCTAC
    TTGAGGCTGA TAAACAGGTG GAGACTAGAA GAGTGCCACC CTAGTGGATG CCTTATTGAT
    CTCTGTATGC AAATGGGTAT TATAATGGTG CTAAAGCAGA CCTGGAATAA TTTCATGGAA
    CTTGGCTACC CGTTAATTCA GAATTGGTGG ACTAGAAGAA AAGTACGACA AGAACATGGG
    CCTGAAAGGA AAATAAGTTT CCCACAATGG GAAAAGGACT ACAACCTTCA GCCGATGAAT
    GCCTACGGAC TCTTTGATGA ATACTTAGAA ATGATTCTTC AGTTTGGATT TACAACTATC
    TTTGTGGCAG CTTTTCCCCT AGCACCACTT CTGGCCTTAC TGAATAATAT AATTGAAATT
    CGACTTGACG CTTATAAATT TGTCACACAG TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA
    GATATAGGAA TTTGGTATGG GATTCTTGAA GGCATTGGAA TTCTCTCCGT TATTACAAAT
    GCATTTGTGA TAGCGATCAC ATCTGACTTT ATCCCTCGCT TGGTATATGC TTATAAGTAT
    GGACCTTGTG CGGGCCAAGG AGAAGCTGGG CAAAAGTGCA TGGTTGGCTA TGTGAATGCC
    AGCTTGTCTG TATTCCGAAT TTCTGACTTT GAGAACAGAT CTGAACCTGA GTCTGATGGC
    AGTGAGTTCT CGGGGACTCC TCTCAAGTAC TGTAGATACC GAGACTACCG TGACCCTCCT
    CATTCACTGG TGCCCTACGG CTACACACTA CAGTTCTGGC ACGTCCTAGC AGCCCGACTG
    GCTTTTATCA TTGTGTTCGA GCACCTCGTG TTTTGTATAA AGCACCTCAT TTCATATCTG
    ATCCCAGACC TCCCAAAAGA TCTAAGGGAT CGAATGAGAA GAGAGAAGTA CTTGATTCAG
    GAGATGATGT ATGAAGCAGA ACTGGAGCGT CTCCAGAAGG AACGAAAGGA GAGGAAGAAA
    AATGGAAAAG CACACCACAA TGAGTGGCCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847509.1
    CDS168..3980
    Translation

    Target ORF information:

    RefSeq Version XM_024991741.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991741.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGTGGCC AAGGATGTCA ATATTCTTTT TGATGAATTA
    GAAGCTGTCA ACAGTCCTTG CAAAGATGAC GACTCTCTCC TTCACCCTGG AAATCTTACC
    AGTACTTCAG ATGATGCTAG CAGGTTGGAA GCCGGGGGAG AGACAGTACC AGAAAAAAAC
    AAATTAAATG GCCTTTACTT CAGAGACGGA AAATGTCGAA TTGACTACAT TCTTGTGTAC
    AGAAAATCCA ACCCCCAGAT GGAAAAGAGA GAAGTATTTG AAAGAAATAT TAGAGCAGAA
    GGATTACAAA TGGAGAAAGA GTCCTCTCTA ATAAATAGTG ACATTATCTT TGTGAAGTTG
    CATGCCCCAT GGGAAGTGCT TGGAAGATAT GCAGAACAAA TGAATGTAAG AATGCCTTTC
    AGGAGAAAAA TCTATTACCT GCCCCGCCGG TACAAGTTCA TGAGCAGGAT CGATAAACAA
    ATAAGCAGGT TTCGGAGATG GTTACCTAAG AAGCCAATGA GGCTGGACAA GGAGACACTG
    CCAGACCTGG AGGAGAATGA CTGCTACACT GCCCCTTTCA GCCAGCAAAG GATCCATCAC
    TTCATCATAC ACAACAAAGA CACTTTCTTC AACAATGCCA CAAGAAGTAG AATTGTACAT
    CATATTTTAC AAAGAATAAA GTATGAAGAA GGAAAAAACA AAATTGGTCT GAATCGTTTG
    CTTACTAATG GCTCCTATGA AGCTGCATTT CCTCTGCACG AGGGAAGTTA CAGAAGTAAA
    AACTCCATCA GAACCCACGG AGCGGTAAAC CACCGACATC TGCTCTATGA GTGCTGGGCG
    TCCTGGGGTG TGTGGTACAA ATACCAGCCT TTGGATCTTG TAAGGCGGTA CTTTGGAGAG
    AAGATTGGGT TGTACTTTGC CTGGTTGGGC TGGTACACCG GCATGCTCTT CCCAGCTGCC
    TTCATTGGAT TGTTTGTCTT TTTGTATGGC GTCATCACTC TGGATCACTG CCAAGTCAGT
    AAAGAAGTCT GCCAAGCTAC AGATATCATC ATGTGTCCTG TGTGTGATAA ATACTGTCCA
    TTCATGAGGC TGTCAGACAG CTGCGTTTAT GCCAAGGTAA CCCATCTTTT TGACAATGGA
    GCCACTGTTT TCTTTGCTGT TTTCATGGCA GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG
    AAGAGACGGA GAGCAGTAAT TGCTTATGAC TGGGATTTGA TAGACTGGGA AGAAGAGGAG
    GAAGAAATAC GGCCCCAGTT TGAAGCCAAG TATTCCAAGA AAGAGAGGAT GAATCCCATT
    TCCGGCAAGC CAGAACCATA TCAAGCATTT GCAGATAAAT GCAGCCGACT TATTGTTTCT
    GCATCTGGAA TATTTTTTAT GATCTGCGTG GTGATCGCCG CTGTGTTCGG GATTGTCATT
    TACAGGGTGG TGACCGTCAG CACTTTCGCA GCCTTTAAGT GGGCATTAAT CAGGAATAAT
    TCTCAGGTTG CAACTACAGG AACTGCTGTG TGCATCAATT TCTGCATCAT TATGCTGCTG
    AATGTGCTCT ATGAAAAGGT TGCTCTTCTT CTGACAAATT TAGAACAGCC TCGCACAGAG
    TCCGAGTGGG AGAACAGCTT CACCCTGAAA ATGTTTCTTT TTCAGTTTGT CAATCTGAAC
    AGCTCCACAT TTTACATCGC GTTCTTCCTC GGAAGATTCA CAGGACACCC AGGTGCCTAC
    TTGAGGCTGA TAAACAGGTG GAGACTAGAA GAGTGCCACC CTAGTGGATG CCTTATTGAT
    CTCTGTATGC AAATGGGTAT TATAATGGTG CTAAAGCAGA CCTGGAATAA TTTCATGGAA
    CTTGGCTACC CGTTAATTCA GAATTGGTGG ACTAGAAGAA AAGTACGACA AGAACATGGG
    CCTGAAAGGA AAATAAGTTT CCCACAATGG GAAAAGGACT ACAACCTTCA GCCGATGAAT
    GCCTACGGAC TCTTTGATGA ATACTTAGAA ATGATTCTTC AGTTTGGATT TACAACTATC
    TTTGTGGCAG CTTTTCCCCT AGCACCACTT CTGGCCTTAC TGAATAATAT AATTGAAATT
    CGACTTGACG CTTATAAATT TGTCACACAG TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA
    GATATAGGAA TTTGGTATGG GATTCTTGAA GGCATTGGAA TTCTCTCCGT TATTACAAAT
    GCATTTGTGA TAGCGATCAC ATCTGACTTT ATCCCTCGCT TGGTATATGC TTATAAGTAT
    GGACCTTGTG CGGGCCAAGG AGAAGCTGGG CAAAAGTGCA TGGTTGGCTA TGTGAATGCC
    AGCTTGTCTG TATTCCGAAT TTCTGACTTT GAGAACAGAT CTGAACCTGA GTCTGATGGC
    AGTGAGTTCT CGGGGACTCC TCTCAAGTAC TGTAGATACC GAGACTACCG TGACCCTCCT
    CATTCACTGG TGCCCTACGG CTACACACTA CAGTTCTGGC ACGTCCTAGC AGCCCGACTG
    GCTTTTATCA TTGTGTTCGA GCACCTCGTG TTTTGTATAA AGCACCTCAT TTCATATCTG
    ATCCCAGACC TCCCAAAAGA TCTAAGGGAT CGAATGAGAA GAGAGAAGTA CTTGATTCAG
    GAGATGATGT ATGAAGCAGA ACTGGAGCGT CTCCAGAAGG AACGAAAGGA GAGGAAGAAA
    AATGGAAAAG CACACCACAA TGAGTGGCCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234384
    Clone ID Related Accession (Same CDS sequence) XM_024991743.1
    Accession Version XM_024991743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847511.1
    CDS168..3950
    Translation

    Target ORF information:

    RefSeq Version XM_024991743.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGT AAAGAAGTCT GCCAAGCTAC AGATATCATC
    ATGTGTCCTG TGTGTGATAA ATACTGTCCA TTCATGAGGC TGTCAGACAG CTGCGTTTAT
    GCCAAGGTAA CCCATCTTTT TGACAATGGA GCCACTGTTT TCTTTGCTGT TTTCATGGCA
    GTCTGGGCAA CAGTTTTCCT AGAGTTTTGG AAGAGACGGA GAGCAGTAAT TGCTTATGAC
    TGGGATTTGA TAGACTGGGA AGAAGAGGAG GAAGAAATAC GGCCCCAGTT TGAAGCCAAG
    TATTCCAAGA AAGAGAGGAT GAATCCCATT TCCGGCAAGC CAGAACCATA TCAAGCATTT
    GCAGATAAAT GCAGCCGACT TATTGTTTCT GCATCTGGAA TATTTTTTAT GATCTGCGTG
    GTGATCGCCG CTGTGTTCGG GATTGTCATT TACAGGGTGG TGACCGTCAG CACTTTCGCA
    GCCTTTAAGT GGGCATTAAT CAGGAATAAT TCTCAGGTTG CAACTACAGG AACTGCTGTG
    TGCATCAATT TCTGCATCAT TATGCTGCTG AATGTGCTCT ATGAAAAGGT TGCTCTTCTT
    CTGACAAATT TAGAACAGCC TCGCACAGAG TCCGAGTGGG AGAACAGCTT CACCCTGAAA
    ATGTTTCTTT TTCAGTTTGT CAATCTGAAC AGCTCCACAT TTTACATCGC GTTCTTCCTC
    GGAAGATTCA CAGGACACCC AGGTGCCTAC TTGAGGCTGA TAAACAGGTG GAGACTAGAA
    GAGTGCCACC CTAGTGGATG CCTTATTGAT CTCTGTATGC AAATGGGTAT TATAATGGTG
    CTAAAGCAGA CCTGGAATAA TTTCATGGAA CTTGGCTACC CGTTAATTCA GAATTGGTGG
    ACTAGAAGAA AAGTACGACA AGAACATGGG CCTGAAAGGA AAATAAGTTT CCCACAATGG
    GAAAAGGACT ACAACCTTCA GCCGATGAAT GCCTACGGAC TCTTTGATGA ATACTTAGAA
    ATGATTCTTC AGTTTGGATT TACAACTATC TTTGTGGCAG CTTTTCCCCT AGCACCACTT
    CTGGCCTTAC TGAATAATAT AATTGAAATT CGACTTGACG CTTATAAATT TGTCACACAG
    TGGAGGCGAC CTTTAGCTTC AAGGGCCAAA GATATAGGAA TTTGGTATGG GATTCTTGAA
    GGCATTGGAA TTCTCTCCGT TATTACAAAT GCATTTGTGA TAGCGATCAC ATCTGACTTT
    ATCCCTCGCT TGGTATATGC TTATAAGTAT GGACCTTGTG CGGGCCAAGG AGAAGCTGGG
    CAAAAGTGCA TGGTTGGCTA TGTGAATGCC AGCTTGTCTG TATTCCGAAT TTCTGACTTT
    GAGAACAGAT CTGAACCTGA GTCTGATGGC AGTGAGTTCT CGGGGACTCC TCTCAAGTAC
    TGTAGATACC GAGACTACCG TGACCCTCCT CATTCACTGG TGCCCTACGG CTACACACTA
    CAGTTCTGGC ACGTCCTAGC AGCCCGACTG GCTTTTATCA TTGTGTTCGA GCACCTCGTG
    TTTTGTATAA AGCACCTCAT TTCATATCTG ATCCCAGACC TCCCAAAAGA TCTAAGGGAT
    CGAATGAGAA GAGAGAAGTA CTTGATTCAG GAGATGATGT ATGAAGCAGA ACTGGAGCGT
    CTCCAGAAGG AACGAAAGGA GAGGAAGAAA AATGGAAAAG CACACCACAA TGAGTGGCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234385
    Clone ID Related Accession (Same CDS sequence) XM_024991744.1
    Accession Version XM_024991744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGTCC TCTCTAATAA ATAGTGACAT TATCTTTGTG
    AAGTTGCATG CCCCATGGGA AGTGCTTGGA AGATATGCAG AACAAATGAA TGTAAGAATG
    CCTTTCAGGA GAAAAATCTA TTACCTGCCC CGCCGGTACA AGTTCATGAG CAGGATCGAT
    AAACAAATAA GCAGGTTTCG GAGATGGTTA CCTAAGAAGC CAATGAGGCT GGACAAGGAG
    ACACTGCCAG ACCTGGAGGA GAATGACTGC TACACTGCCC CTTTCAGCCA GCAAAGGATC
    CATCACTTCA TCATACACAA CAAAGACACT TTCTTCAACA ATGCCACAAG AAGTAGAATT
    GTACATCATA TTTTACAAAG AATAAAGTAT GAAGAAGGAA AAAACAAAAT TGGTCTGAAT
    CGTTTGCTTA CTAATGGCTC CTATGAAGCT GCATTTCCTC TGCACGAGGG AAGTTACAGA
    AGTAAAAACT CCATCAGAAC CCACGGAGCG GTAAACCACC GACATCTGCT CTATGAGTGC
    TGGGCGTCCT GGGGTGTGTG GTACAAATAC CAGCCTTTGG ATCTTGTAAG GCGGTACTTT
    GGAGAGAAGA TTGGGTTGTA CTTTGCCTGG TTGGGCTGGT ACACCGGCAT GCTCTTCCCA
    GCTGCCTTCA TTGGATTGTT TGTCTTTTTG TATGGCGTCA TCACTCTGGA TCACTGCCAA
    GTCAGTAAAG AAGTCTGCCA AGCTACAGAT ATCATCATGT GTCCTGTGTG TGATAAATAC
    TGTCCATTCA TGAGGCTGTC AGACAGCTGC GTTTATGCCA AGGTAACCCA TCTTTTTGAC
    AATGGAGCCA CTGTTTTCTT TGCTGTTTTC ATGGCAGTCT GGGCAACAGT TTTCCTAGAG
    TTTTGGAAGA GACGGAGAGC AGTAATTGCT TATGACTGGG ATTTGATAGA CTGGGAAGAA
    GAGGAGGAAG AAATACGGCC CCAGTTTGAA GCCAAGTATT CCAAGAAAGA GAGGATGAAT
    CCCATTTCCG GCAAGCCAGA ACCATATCAA GCATTTGCAG ATAAATGCAG CCGACTTATT
    GTTTCTGCAT CTGGAATATT TTTTATGATC TGCGTGGTGA TCGCCGCTGT GTTCGGGATT
    GTCATTTACA GGGTGGTGAC CGTCAGCACT TTCGCAGCCT TTAAGTGGGC ATTAATCAGG
    AATAATTCTC AGGTTGCAAC TACAGGAACT GCTGTGTGCA TCAATTTCTG CATCATTATG
    CTGCTGAATG TGCTCTATGA AAAGGTTGCT CTTCTTCTGA CAAATTTAGA ACAGCCTCGC
    ACAGAGTCCG AGTGGGAGAA CAGCTTCACC CTGAAAATGT TTCTTTTTCA GTTTGTCAAT
    CTGAACAGCT CCACATTTTA CATCGCGTTC TTCCTCGGAA GATTCACAGG ACACCCAGGT
    GCCTACTTGA GGCTGATAAA CAGGTGGAGA CTAGAAGAGT GCCACCCTAG TGGATGCCTT
    ATTGATCTCT GTATGCAAAT GGGTATTATA ATGGTGCTAA AGCAGACCTG GAATAATTTC
    ATGGAACTTG GCTACCCGTT AATTCAGAAT TGGTGGACTA GAAGAAAAGT ACGACAAGAA
    CATGGGCCTG AAAGGAAAAT AAGTTTCCCA CAATGGGAAA AGGACTACAA CCTTCAGCCG
    ATGAATGCCT ACGGACTCTT TGATGAATAC TTAGAAATGA TTCTTCAGTT TGGATTTACA
    ACTATCTTTG TGGCAGCTTT TCCCCTAGCA CCACTTCTGG CCTTACTGAA TAATATAATT
    GAAATTCGAC TTGACGCTTA TAAATTTGTC ACACAGTGGA GGCGACCTTT AGCTTCAAGG
    GCCAAAGATA TAGGAATTTG GTATGGGATT CTTGAAGGCA TTGGAATTCT CTCCGTTATT
    ACAAATGCAT TTGTGATAGC GATCACATCT GACTTTATCC CTCGCTTGGT ATATGCTTAT
    AAGTATGGAC CTTGTGCGGG CCAAGGAGAA GCTGGGCAAA AGTGCATGGT TGGCTATGTG
    AATGCCAGCT TGTCTGTATT CCGAATTTCT GACTTTGAGA ACAGATCTGA ACCTGAGTCT
    GATGGCAGTG AGTTCTCGGG GACTCCTCTC AAGTACTGTA GATACCGAGA CTACCGTGAC
    CCTCCTCATT CACTGGTGCC CTACGGCTAC ACACTACAGT TCTGGCACGT CCTAGCAGCC
    CGACTGGCTT TTATCATTGT GTTCGAGCAC CTCGTGTTTT GTATAAAGCA CCTCATTTCA
    TATCTGATCC CAGACCTCCC AAAAGATCTA AGGGATCGAA TGAGAAGAGA GAAGTACTTG
    ATTCAGGAGA TGATGTATGA AGCAGAACTG GAGCGTCTCC AGAAGGAACG AAAGGAGAGG
    AAGAAAAATG GAAAAGCACA CCACAATGAG TGGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847512.1
    CDS170..3928
    Translation

    Target ORF information:

    RefSeq Version XM_024991744.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGTCC TCTCTAATAA ATAGTGACAT TATCTTTGTG
    AAGTTGCATG CCCCATGGGA AGTGCTTGGA AGATATGCAG AACAAATGAA TGTAAGAATG
    CCTTTCAGGA GAAAAATCTA TTACCTGCCC CGCCGGTACA AGTTCATGAG CAGGATCGAT
    AAACAAATAA GCAGGTTTCG GAGATGGTTA CCTAAGAAGC CAATGAGGCT GGACAAGGAG
    ACACTGCCAG ACCTGGAGGA GAATGACTGC TACACTGCCC CTTTCAGCCA GCAAAGGATC
    CATCACTTCA TCATACACAA CAAAGACACT TTCTTCAACA ATGCCACAAG AAGTAGAATT
    GTACATCATA TTTTACAAAG AATAAAGTAT GAAGAAGGAA AAAACAAAAT TGGTCTGAAT
    CGTTTGCTTA CTAATGGCTC CTATGAAGCT GCATTTCCTC TGCACGAGGG AAGTTACAGA
    AGTAAAAACT CCATCAGAAC CCACGGAGCG GTAAACCACC GACATCTGCT CTATGAGTGC
    TGGGCGTCCT GGGGTGTGTG GTACAAATAC CAGCCTTTGG ATCTTGTAAG GCGGTACTTT
    GGAGAGAAGA TTGGGTTGTA CTTTGCCTGG TTGGGCTGGT ACACCGGCAT GCTCTTCCCA
    GCTGCCTTCA TTGGATTGTT TGTCTTTTTG TATGGCGTCA TCACTCTGGA TCACTGCCAA
    GTCAGTAAAG AAGTCTGCCA AGCTACAGAT ATCATCATGT GTCCTGTGTG TGATAAATAC
    TGTCCATTCA TGAGGCTGTC AGACAGCTGC GTTTATGCCA AGGTAACCCA TCTTTTTGAC
    AATGGAGCCA CTGTTTTCTT TGCTGTTTTC ATGGCAGTCT GGGCAACAGT TTTCCTAGAG
    TTTTGGAAGA GACGGAGAGC AGTAATTGCT TATGACTGGG ATTTGATAGA CTGGGAAGAA
    GAGGAGGAAG AAATACGGCC CCAGTTTGAA GCCAAGTATT CCAAGAAAGA GAGGATGAAT
    CCCATTTCCG GCAAGCCAGA ACCATATCAA GCATTTGCAG ATAAATGCAG CCGACTTATT
    GTTTCTGCAT CTGGAATATT TTTTATGATC TGCGTGGTGA TCGCCGCTGT GTTCGGGATT
    GTCATTTACA GGGTGGTGAC CGTCAGCACT TTCGCAGCCT TTAAGTGGGC ATTAATCAGG
    AATAATTCTC AGGTTGCAAC TACAGGAACT GCTGTGTGCA TCAATTTCTG CATCATTATG
    CTGCTGAATG TGCTCTATGA AAAGGTTGCT CTTCTTCTGA CAAATTTAGA ACAGCCTCGC
    ACAGAGTCCG AGTGGGAGAA CAGCTTCACC CTGAAAATGT TTCTTTTTCA GTTTGTCAAT
    CTGAACAGCT CCACATTTTA CATCGCGTTC TTCCTCGGAA GATTCACAGG ACACCCAGGT
    GCCTACTTGA GGCTGATAAA CAGGTGGAGA CTAGAAGAGT GCCACCCTAG TGGATGCCTT
    ATTGATCTCT GTATGCAAAT GGGTATTATA ATGGTGCTAA AGCAGACCTG GAATAATTTC
    ATGGAACTTG GCTACCCGTT AATTCAGAAT TGGTGGACTA GAAGAAAAGT ACGACAAGAA
    CATGGGCCTG AAAGGAAAAT AAGTTTCCCA CAATGGGAAA AGGACTACAA CCTTCAGCCG
    ATGAATGCCT ACGGACTCTT TGATGAATAC TTAGAAATGA TTCTTCAGTT TGGATTTACA
    ACTATCTTTG TGGCAGCTTT TCCCCTAGCA CCACTTCTGG CCTTACTGAA TAATATAATT
    GAAATTCGAC TTGACGCTTA TAAATTTGTC ACACAGTGGA GGCGACCTTT AGCTTCAAGG
    GCCAAAGATA TAGGAATTTG GTATGGGATT CTTGAAGGCA TTGGAATTCT CTCCGTTATT
    ACAAATGCAT TTGTGATAGC GATCACATCT GACTTTATCC CTCGCTTGGT ATATGCTTAT
    AAGTATGGAC CTTGTGCGGG CCAAGGAGAA GCTGGGCAAA AGTGCATGGT TGGCTATGTG
    AATGCCAGCT TGTCTGTATT CCGAATTTCT GACTTTGAGA ACAGATCTGA ACCTGAGTCT
    GATGGCAGTG AGTTCTCGGG GACTCCTCTC AAGTACTGTA GATACCGAGA CTACCGTGAC
    CCTCCTCATT CACTGGTGCC CTACGGCTAC ACACTACAGT TCTGGCACGT CCTAGCAGCC
    CGACTGGCTT TTATCATTGT GTTCGAGCAC CTCGTGTTTT GTATAAAGCA CCTCATTTCA
    TATCTGATCC CAGACCTCCC AAAAGATCTA AGGGATCGAA TGAGAAGAGA GAAGTACTTG
    ATTCAGGAGA TGATGTATGA AGCAGAACTG GAGCGTCTCC AGAAGGAACG AAAGGAGAGG
    AAGAAAAATG GAAAAGCACA CCACAATGAG TGGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234386
    Clone ID Related Accession (Same CDS sequence) XM_024991745.1
    Accession Version XM_024991745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGCTT CATCATACAC
    AACAAAGACA CTTTCTTCAA CAATGCCACA AGAAGTAGAA TTGTACATCA TATTTTACAA
    AGAATAAAGT ATGAAGAAGG AAAAAACAAA ATTGGTCTGA ATCGTTTGCT TACTAATGGC
    TCCTATGAAG CTGCATTTCC TCTGCACGAG GGAAGTTACA GAAGTAAAAA CTCCATCAGA
    ACCCACGGAG CGGTAAACCA CCGACATCTG CTCTATGAGT GCTGGGCGTC CTGGGGTGTG
    TGGTACAAAT ACCAGCCTTT GGATCTTGTA AGGCGGTACT TTGGAGAGAA GATTGGGTTG
    TACTTTGCCT GGTTGGGCTG GTACACCGGC ATGCTCTTCC CAGCTGCCTT CATTGGATTG
    TTTGTCTTTT TGTATGGCGT CATCACTCTG GATCACTGCC AAGTCAGTAA AGAAGTCTGC
    CAAGCTACAG ATATCATCAT GTGTCCTGTG TGTGATAAAT ACTGTCCATT CATGAGGCTG
    TCAGACAGCT GCGTTTATGC CAAGGTAACC CATCTTTTTG ACAATGGAGC CACTGTTTTC
    TTTGCTGTTT TCATGGCAGT CTGGGCAACA GTTTTCCTAG AGTTTTGGAA GAGACGGAGA
    GCAGTAATTG CTTATGACTG GGATTTGATA GACTGGGAAG AAGAGGAGGA AGAAATACGG
    CCCCAGTTTG AAGCCAAGTA TTCCAAGAAA GAGAGGATGA ATCCCATTTC CGGCAAGCCA
    GAACCATATC AAGCATTTGC AGATAAATGC AGCCGACTTA TTGTTTCTGC ATCTGGAATA
    TTTTTTATGA TCTGCGTGGT GATCGCCGCT GTGTTCGGGA TTGTCATTTA CAGGGTGGTG
    ACCGTCAGCA CTTTCGCAGC CTTTAAGTGG GCATTAATCA GGAATAATTC TCAGGTTGCA
    ACTACAGGAA CTGCTGTGTG CATCAATTTC TGCATCATTA TGCTGCTGAA TGTGCTCTAT
    GAAAAGGTTG CTCTTCTTCT GACAAATTTA GAACAGCCTC GCACAGAGTC CGAGTGGGAG
    AACAGCTTCA CCCTGAAAAT GTTTCTTTTT CAGTTTGTCA ATCTGAACAG CTCCACATTT
    TACATCGCGT TCTTCCTCGG AAGATTCACA GGACACCCAG GTGCCTACTT GAGGCTGATA
    AACAGGTGGA GACTAGAAGA GTGCCACCCT AGTGGATGCC TTATTGATCT CTGTATGCAA
    ATGGGTATTA TAATGGTGCT AAAGCAGACC TGGAATAATT TCATGGAACT TGGCTACCCG
    TTAATTCAGA ATTGGTGGAC TAGAAGAAAA GTACGACAAG AACATGGGCC TGAAAGGAAA
    ATAAGTTTCC CACAATGGGA AAAGGACTAC AACCTTCAGC CGATGAATGC CTACGGACTC
    TTTGATGAAT ACTTAGAAAT GATTCTTCAG TTTGGATTTA CAACTATCTT TGTGGCAGCT
    TTTCCCCTAG CACCACTTCT GGCCTTACTG AATAATATAA TTGAAATTCG ACTTGACGCT
    TATAAATTTG TCACACAGTG GAGGCGACCT TTAGCTTCAA GGGCCAAAGA TATAGGAATT
    TGGTATGGGA TTCTTGAAGG CATTGGAATT CTCTCCGTTA TTACAAATGC ATTTGTGATA
    GCGATCACAT CTGACTTTAT CCCTCGCTTG GTATATGCTT ATAAGTATGG ACCTTGTGCG
    GGCCAAGGAG AAGCTGGGCA AAAGTGCATG GTTGGCTATG TGAATGCCAG CTTGTCTGTA
    TTCCGAATTT CTGACTTTGA GAACAGATCT GAACCTGAGT CTGATGGCAG TGAGTTCTCG
    GGGACTCCTC TCAAGTACTG TAGATACCGA GACTACCGTG ACCCTCCTCA TTCACTGGTG
    CCCTACGGCT ACACACTACA GTTCTGGCAC GTCCTAGCAG CCCGACTGGC TTTTATCATT
    GTGTTCGAGC ACCTCGTGTT TTGTATAAAG CACCTCATTT CATATCTGAT CCCAGACCTC
    CCAAAAGATC TAAGGGATCG AATGAGAAGA GAGAAGTACT TGATTCAGGA GATGATGTAT
    GAAGCAGAAC TGGAGCGTCT CCAGAAGGAA CGAAAGGAGA GGAAGAAAAA TGGAAAAGCA
    CACCACAATG AGTGGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847513.1
    CDS168..3908
    Translation

    Target ORF information:

    RefSeq Version XM_024991745.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGCTT CATCATACAC
    AACAAAGACA CTTTCTTCAA CAATGCCACA AGAAGTAGAA TTGTACATCA TATTTTACAA
    AGAATAAAGT ATGAAGAAGG AAAAAACAAA ATTGGTCTGA ATCGTTTGCT TACTAATGGC
    TCCTATGAAG CTGCATTTCC TCTGCACGAG GGAAGTTACA GAAGTAAAAA CTCCATCAGA
    ACCCACGGAG CGGTAAACCA CCGACATCTG CTCTATGAGT GCTGGGCGTC CTGGGGTGTG
    TGGTACAAAT ACCAGCCTTT GGATCTTGTA AGGCGGTACT TTGGAGAGAA GATTGGGTTG
    TACTTTGCCT GGTTGGGCTG GTACACCGGC ATGCTCTTCC CAGCTGCCTT CATTGGATTG
    TTTGTCTTTT TGTATGGCGT CATCACTCTG GATCACTGCC AAGTCAGTAA AGAAGTCTGC
    CAAGCTACAG ATATCATCAT GTGTCCTGTG TGTGATAAAT ACTGTCCATT CATGAGGCTG
    TCAGACAGCT GCGTTTATGC CAAGGTAACC CATCTTTTTG ACAATGGAGC CACTGTTTTC
    TTTGCTGTTT TCATGGCAGT CTGGGCAACA GTTTTCCTAG AGTTTTGGAA GAGACGGAGA
    GCAGTAATTG CTTATGACTG GGATTTGATA GACTGGGAAG AAGAGGAGGA AGAAATACGG
    CCCCAGTTTG AAGCCAAGTA TTCCAAGAAA GAGAGGATGA ATCCCATTTC CGGCAAGCCA
    GAACCATATC AAGCATTTGC AGATAAATGC AGCCGACTTA TTGTTTCTGC ATCTGGAATA
    TTTTTTATGA TCTGCGTGGT GATCGCCGCT GTGTTCGGGA TTGTCATTTA CAGGGTGGTG
    ACCGTCAGCA CTTTCGCAGC CTTTAAGTGG GCATTAATCA GGAATAATTC TCAGGTTGCA
    ACTACAGGAA CTGCTGTGTG CATCAATTTC TGCATCATTA TGCTGCTGAA TGTGCTCTAT
    GAAAAGGTTG CTCTTCTTCT GACAAATTTA GAACAGCCTC GCACAGAGTC CGAGTGGGAG
    AACAGCTTCA CCCTGAAAAT GTTTCTTTTT CAGTTTGTCA ATCTGAACAG CTCCACATTT
    TACATCGCGT TCTTCCTCGG AAGATTCACA GGACACCCAG GTGCCTACTT GAGGCTGATA
    AACAGGTGGA GACTAGAAGA GTGCCACCCT AGTGGATGCC TTATTGATCT CTGTATGCAA
    ATGGGTATTA TAATGGTGCT AAAGCAGACC TGGAATAATT TCATGGAACT TGGCTACCCG
    TTAATTCAGA ATTGGTGGAC TAGAAGAAAA GTACGACAAG AACATGGGCC TGAAAGGAAA
    ATAAGTTTCC CACAATGGGA AAAGGACTAC AACCTTCAGC CGATGAATGC CTACGGACTC
    TTTGATGAAT ACTTAGAAAT GATTCTTCAG TTTGGATTTA CAACTATCTT TGTGGCAGCT
    TTTCCCCTAG CACCACTTCT GGCCTTACTG AATAATATAA TTGAAATTCG ACTTGACGCT
    TATAAATTTG TCACACAGTG GAGGCGACCT TTAGCTTCAA GGGCCAAAGA TATAGGAATT
    TGGTATGGGA TTCTTGAAGG CATTGGAATT CTCTCCGTTA TTACAAATGC ATTTGTGATA
    GCGATCACAT CTGACTTTAT CCCTCGCTTG GTATATGCTT ATAAGTATGG ACCTTGTGCG
    GGCCAAGGAG AAGCTGGGCA AAAGTGCATG GTTGGCTATG TGAATGCCAG CTTGTCTGTA
    TTCCGAATTT CTGACTTTGA GAACAGATCT GAACCTGAGT CTGATGGCAG TGAGTTCTCG
    GGGACTCCTC TCAAGTACTG TAGATACCGA GACTACCGTG ACCCTCCTCA TTCACTGGTG
    CCCTACGGCT ACACACTACA GTTCTGGCAC GTCCTAGCAG CCCGACTGGC TTTTATCATT
    GTGTTCGAGC ACCTCGTGTT TTGTATAAAG CACCTCATTT CATATCTGAT CCCAGACCTC
    CCAAAAGATC TAAGGGATCG AATGAGAAGA GAGAAGTACT TGATTCAGGA GATGATGTAT
    GAAGCAGAAC TGGAGCGTCT CCAGAAGGAA CGAAAGGAGA GGAAGAAAAA TGGAAAAGCA
    CACCACAATG AGTGGCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234387
    Clone ID Related Accession (Same CDS sequence) XM_024991746.1
    Accession Version XM_024991746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGG CGGTACTTTG GAGAGAAGAT TGGGTTGTAC
    TTTGCCTGGT TGGGCTGGTA CACCGGCATG CTCTTCCCAG CTGCCTTCAT TGGATTGTTT
    GTCTTTTTGT ATGGCGTCAT CACTCTGGAT CACTGCCAAG TCAGTAAAGA AGTCTGCCAA
    GCTACAGATA TCATCATGTG TCCTGTGTGT GATAAATACT GTCCATTCAT GAGGCTGTCA
    GACAGCTGCG TTTATGCCAA GGTAACCCAT CTTTTTGACA ATGGAGCCAC TGTTTTCTTT
    GCTGTTTTCA TGGCAGTCTG GGCAACAGTT TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA
    GTAATTGCTT ATGACTGGGA TTTGATAGAC TGGGAAGAAG AGGAGGAAGA AATACGGCCC
    CAGTTTGAAG CCAAGTATTC CAAGAAAGAG AGGATGAATC CCATTTCCGG CAAGCCAGAA
    CCATATCAAG CATTTGCAGA TAAATGCAGC CGACTTATTG TTTCTGCATC TGGAATATTT
    TTTATGATCT GCGTGGTGAT CGCCGCTGTG TTCGGGATTG TCATTTACAG GGTGGTGACC
    GTCAGCACTT TCGCAGCCTT TAAGTGGGCA TTAATCAGGA ATAATTCTCA GGTTGCAACT
    ACAGGAACTG CTGTGTGCAT CAATTTCTGC ATCATTATGC TGCTGAATGT GCTCTATGAA
    AAGGTTGCTC TTCTTCTGAC AAATTTAGAA CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC
    AGCTTCACCC TGAAAATGTT TCTTTTTCAG TTTGTCAATC TGAACAGCTC CACATTTTAC
    ATCGCGTTCT TCCTCGGAAG ATTCACAGGA CACCCAGGTG CCTACTTGAG GCTGATAAAC
    AGGTGGAGAC TAGAAGAGTG CCACCCTAGT GGATGCCTTA TTGATCTCTG TATGCAAATG
    GGTATTATAA TGGTGCTAAA GCAGACCTGG AATAATTTCA TGGAACTTGG CTACCCGTTA
    ATTCAGAATT GGTGGACTAG AAGAAAAGTA CGACAAGAAC ATGGGCCTGA AAGGAAAATA
    AGTTTCCCAC AATGGGAAAA GGACTACAAC CTTCAGCCGA TGAATGCCTA CGGACTCTTT
    GATGAATACT TAGAAATGAT TCTTCAGTTT GGATTTACAA CTATCTTTGT GGCAGCTTTT
    CCCCTAGCAC CACTTCTGGC CTTACTGAAT AATATAATTG AAATTCGACT TGACGCTTAT
    AAATTTGTCA CACAGTGGAG GCGACCTTTA GCTTCAAGGG CCAAAGATAT AGGAATTTGG
    TATGGGATTC TTGAAGGCAT TGGAATTCTC TCCGTTATTA CAAATGCATT TGTGATAGCG
    ATCACATCTG ACTTTATCCC TCGCTTGGTA TATGCTTATA AGTATGGACC TTGTGCGGGC
    CAAGGAGAAG CTGGGCAAAA GTGCATGGTT GGCTATGTGA ATGCCAGCTT GTCTGTATTC
    CGAATTTCTG ACTTTGAGAA CAGATCTGAA CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG
    ACTCCTCTCA AGTACTGTAG GTTTGTGGCA ATCCTGGGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847514.1
    CDS169..3810
    Translation

    Target ORF information:

    RefSeq Version XM_024991746.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 4 (ANO4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCCCCAG GCTTGCTTTC CAGTGGAACT TCCCTAGAAA GGGAGCTGGA GGTTCCAGGT 
    CCCCGAAGCC GTGGCGCGCT CGGAACTCCC GATCCCGCCT TTGCCCGCGT CACCTCTGCC
    GCACCCACCC CGAGGGGCAC CCCTCTTCGG CGTGGACCCA GCCAGGTCCT GCCCGCCGCC
    CGCCGGGTGT GGGCGCAGTC ACCCGGTACC TCCGCCCGGC ACCTCCCACC GCCGGCTTTG
    CCCCTCCTCT CTCGAGGTTG CGCGCCCTCC CGGCAGGTTC AATCCCCGCG GGTACCCGGA
    GCACCGCGGC CCGTGTGCGT CCTCGCTCCC CACGCCCTAG CAGCCCAGAC CCGGCCCCGG
    GCACCTGCGC GCCGCCGGCT CCTCTCCGAG CGGCCGCGGC CGGCGGACTG GGCATGCTCA
    GCTGTGGGGC GGCCGGGCGC CGCGGGAGCC GAGCGCATGC CTTCGCGGGG CCGGACGCCC
    GCCGTCCAGG GGCGCGGCAC GCAGGCGGCC GCTGTCGCCG ACGCCCGGGC CAGCCTGCGC
    AGGACGCGGG CCAGGATGGC CTCTCTCACG GCATACGACA AAGATATAAG CAGTGGGAGT
    TATAACCTTT CCTCTGCAAC GACCGGCAGC TACTACAGTT GTGTCAGCCA GGTCACTATA
    GGTTATGATG TTGCAGGGCC TGTTGCAATT GGGCTCACCC GCTGGAAAAA TCCCTATGCA
    CAAGGCAGAC CCACCCTACC ATCAGATCTG CCTCTCTTCA TCTCCAAAGA TGCAGGCGTC
    CCAGTGTATC ACCGATCCCT CACTCGCAAG ACGGAGCTGG CCACGCCTCT GTCTTGCCCA
    GCCTCACTGT CCATCACTCC AGTGCCTTCT TACAGCAGCA GCAGCCAGGA AACCCTGAGC
    CAGGACACAA CAGGTTTAAG TTTATCTATT CATGGGGCTG AAAAGCGTTT GCAAATCCAC
    CAACGGCGAA GTGTGGCAAG CCGCCCAGGG TCACGTCGCC TGCCCGTGGT CAGGCATTCC
    TCACTTCCAC CAGGCAGAAG GTCAGTAAAA ATGGAGACGA GCTCTTCTGG AATCACTAAT
    GGGAGAACCA GAGTCTTCCA CCCAGAAGGA GGTGTGGATT TGCAAAGCTA CCAGCTGGAT
    ATGCAAATAC TACCTGACGG GCCAAGGAGT GATGTGGACT TTTCAGAGAT TCTTAATGCA
    ATACAAGAAA TGGCCAAGGA TGTCAATATT CTTTTTGATG AATTAGAAGC TGTCAACAGT
    CCTTGCAAAG ATGACGACTC TCTCCTTCAC CCTGGAAATC TTACCAGTAC TTCAGATGAT
    GCTAGCAGGT TGGAAGCCGG GGGAGAGACA GTACCAGAAA AAAACAAATT AAATGGCCTT
    TACTTCAGAG ACGGAAAATG TCGAATTGAC TACATTCTTG TGTACAGAAA ATCCAACCCC
    CAGATGGAAA AGAGAGAAGT ATTTGAAAGA AATATTAGAG CAGAAGGATT ACAAATGGAG
    AAAGAGTCCT CTCTAATAAA TAGTGACATT ATCTTTGTGA AGTTGCATGC CCCATGGGAA
    GTGCTTGGAA GATATGCAGA ACAAATGAAT GTAAGAATGC CTTTCAGGAG AAAAATCTAT
    TACCTGCCCC GCCGGTACAA GTTCATGAGC AGGATCGATA AACAAATAAG CAGGTTTCGG
    AGATGGTTAC CTAAGAAGCC AATGAGGCTG GACAAGGAGA CACTGCCAGA CCTGGAGGAG
    AATGACTGCT ACACTGCCCC TTTCAGCCAG CAAAGGATCC ATCACTTCAT CATACACAAC
    AAAGACACTT TCTTCAACAA TGCCACAAGA AGTAGAATTG TACATCATAT TTTACAAAGA
    ATAAAGTATG AAGAAGGAAA AAACAAAATT GGTCTGAATC GTTTGCTTAC TAATGGCTCC
    TATGAAGCTG CATTTCCTCT GCACGAGGGA AGTTACAGAA GTAAAAACTC CATCAGAACC
    CACGGAGCGG TAAACCACCG ACATCTGCTC TATGAGTGCT GGGCGTCCTG GGGTGTGTGG
    TACAAATACC AGCCTTTGGA TCTTGTAAGG CGGTACTTTG GAGAGAAGAT TGGGTTGTAC
    TTTGCCTGGT TGGGCTGGTA CACCGGCATG CTCTTCCCAG CTGCCTTCAT TGGATTGTTT
    GTCTTTTTGT ATGGCGTCAT CACTCTGGAT CACTGCCAAG TCAGTAAAGA AGTCTGCCAA
    GCTACAGATA TCATCATGTG TCCTGTGTGT GATAAATACT GTCCATTCAT GAGGCTGTCA
    GACAGCTGCG TTTATGCCAA GGTAACCCAT CTTTTTGACA ATGGAGCCAC TGTTTTCTTT
    GCTGTTTTCA TGGCAGTCTG GGCAACAGTT TTCCTAGAGT TTTGGAAGAG ACGGAGAGCA
    GTAATTGCTT ATGACTGGGA TTTGATAGAC TGGGAAGAAG AGGAGGAAGA AATACGGCCC
    CAGTTTGAAG CCAAGTATTC CAAGAAAGAG AGGATGAATC CCATTTCCGG CAAGCCAGAA
    CCATATCAAG CATTTGCAGA TAAATGCAGC CGACTTATTG TTTCTGCATC TGGAATATTT
    TTTATGATCT GCGTGGTGAT CGCCGCTGTG TTCGGGATTG TCATTTACAG GGTGGTGACC
    GTCAGCACTT TCGCAGCCTT TAAGTGGGCA TTAATCAGGA ATAATTCTCA GGTTGCAACT
    ACAGGAACTG CTGTGTGCAT CAATTTCTGC ATCATTATGC TGCTGAATGT GCTCTATGAA
    AAGGTTGCTC TTCTTCTGAC AAATTTAGAA CAGCCTCGCA CAGAGTCCGA GTGGGAGAAC
    AGCTTCACCC TGAAAATGTT TCTTTTTCAG TTTGTCAATC TGAACAGCTC CACATTTTAC
    ATCGCGTTCT TCCTCGGAAG ATTCACAGGA CACCCAGGTG CCTACTTGAG GCTGATAAAC
    AGGTGGAGAC TAGAAGAGTG CCACCCTAGT GGATGCCTTA TTGATCTCTG TATGCAAATG
    GGTATTATAA TGGTGCTAAA GCAGACCTGG AATAATTTCA TGGAACTTGG CTACCCGTTA
    ATTCAGAATT GGTGGACTAG AAGAAAAGTA CGACAAGAAC ATGGGCCTGA AAGGAAAATA
    AGTTTCCCAC AATGGGAAAA GGACTACAAC CTTCAGCCGA TGAATGCCTA CGGACTCTTT
    GATGAATACT TAGAAATGAT TCTTCAGTTT GGATTTACAA CTATCTTTGT GGCAGCTTTT
    CCCCTAGCAC CACTTCTGGC CTTACTGAAT AATATAATTG AAATTCGACT TGACGCTTAT
    AAATTTGTCA CACAGTGGAG GCGACCTTTA GCTTCAAGGG CCAAAGATAT AGGAATTTGG
    TATGGGATTC TTGAAGGCAT TGGAATTCTC TCCGTTATTA CAAATGCATT TGTGATAGCG
    ATCACATCTG ACTTTATCCC TCGCTTGGTA TATGCTTATA AGTATGGACC TTGTGCGGGC
    CAAGGAGAAG CTGGGCAAAA GTGCATGGTT GGCTATGTGA ATGCCAGCTT GTCTGTATTC
    CGAATTTCTG ACTTTGAGAA CAGATCTGAA CCTGAGTCTG ATGGCAGTGA GTTCTCGGGG
    ACTCCTCTCA AGTACTGTAG GTTTGTGGCA ATCCTGGGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.