HECW1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW1
Entrez Gene ID	514243
Full Name	E3 ubiquitin-protein ligase HECW1
Synonyms	HECW1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024991128.1	XP_024846896.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_003585989.4	XP_003586037.3	E3 ubiquitin-protein ligase HECW1
XM_024991127.1	XP_024846895.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_015463079.1	XP_015318565.1	E3 ubiquitin-protein ligase HECW1
XM_003585989.5	XP_003586037.4	E3 ubiquitin-protein ligase HECW1 isoform X1

Pathways from BioSystems
Gene Ontology

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa48879	XM_015463079.1 Latest version!	Bos taurus E3 ubiquitin-protein ligase HECW1 (LOC514243), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa48879	XM_003585989.4 Latest version!	Bos taurus E3 ubiquitin-protein ligase HECW1 (LOC514243), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa234009	XM_003585989.5 Latest version!	Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa234010	XM_024991127.1 Latest version!	Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa03947	XM_024991128.1 Latest version!	Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW1 ( XM_003585989.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586037.3 Bos taurus E3 ubiquitin-protein ligase HECW1 (LOC514243), mRNA.

Price & Availability↑

CloneID	OBa48879
Clone ID Related Accession (Same CDS sequence)	XM_003585989.4 , XM_015463079.1
Accession Version	XM_003585989.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003585989.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGAT CTGGATCCAT CCAGCAGATG GAGCAGCTCA ACAGGCGGTA CCAAAACATT 
CAGCGAACCA TTGCTACAGA GAGGCCTGAA GAAGATTCTG GTAGCCAAAT TTGTGATCCA 
GTTCCAGCAG GAGGCGGTGG AGGAGGTGGG AGTGACTCAG AGGCTGAATC TTCGAGCTTA 
GATCTGAGAA GAGATGGGTC ACTTTCTCCA GTGAACTCAC AAAAAATCAC CTTGCTGCTA 
CAGTCTCCAG CGGTCAAATT CATCACCAAC CCTGAGTTCT TCACTGTACT GCATGCCAAC 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTGA AGCACATGAT CCTCAAAGTC 
CGGCGGGATG CGCGCAACTT CGAACGCTAC CAGCACAACC GAGACTTGGT GAATTTCATC 
AACATGTTCG CGGACACGCG GCTGGAATTG CCCCGGGGCT GGGAGATCAA AACGGACCAG 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CCACTTTTAT TGACCCCCGA 
ATCCCTCTTC AGAACGGTCG TCTTCCCAAC CATCTGACTC ATCGACAGCA TCTCCAGAGG 
CTCCGAAGTT ACAGTGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCTTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGACTCA 
TTGCCACTGG CATATAACGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA TATTTTTGAA 
ATGCTGCAAG AGCGCCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TACATTCGGA CTGAGGGTAA TCATGGGCTT GAAAAGTTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA GGAAGAGATT ATGTCCTATG TCCCCCTGCA GGCCGCCTTC 
CACCCTGGGT ACAGCTTCTC TCCCCGCTGC TCACCCTGCT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCCTACA GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATCA TTCGTCGAGA CCACCTGTTG GAGGGAACCT TCAATCAGGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGCG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCGAG AGAATTCTTC TTCCTTCTGT CTCAGGAGCT CTTTAACCCT 
TACTACGGAC TCTTCGAGTA CTCTGCCAAT GACACCTACA CCGTGCAGAT CAGCCCCATG 
TCTGCCTTTG TAGAAAACCA TCTTGAATGG TTCAGGTTCA GTGGTCGAAT CCTAGGCCTG 
GCTCTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GACCCTTCTA TAAGGCGCTC 
CTGAGACTAC CCTGTGATTT GAGTGACCTG GAATATCTAG ATGAGGAATT CCACCAGAGC 
TTGCAATGGA TGAAGGACAA CAATATCACA GACATCCTCG ACCTCACGTT CACGGTTAAT 
GAAGAGGTTT TTGGCCAGGT AACGGAGAGG GAGTTAAAAT CTGGAGGTGC CAACACACAG 
GTGACAGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TGAGGTGGCG GGTAGAGCGC 
GGCGTGGTGC AGCAGACCGA GGCACTGGTG CGGGGCTTCT ACGAGGTGGT GGACTCAAGG 
CTGGTCTCCG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TCGCTGGCAC AGCAGAGATT 
GACCTGGCCG ACTGGCGGAA CAACACTGAG TACCGGGGAG GTTATCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCGGTGGAG CGTTTCAACA ATGAGCAGAG ACTCAGATTA 
CTGCAGTTCG TCACAGGAAC ATCAAGCGTG CCCTACGAAG GCTTTGCAGC CCTGCGAGGA 
AGCAACGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACATC TCTTCCCAGG 
GCACACACGT GCTTCAATCG ATTGGATCTT CCACCTTATC CCTCGTACTC CATGTTGTAT 
GAAAAGCTGT TAACGGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586037.3
CDS	802..3012
Translation	MRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGSQICDPVPAGGGGGGGSDSEAESSSLDLRRDGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHDSLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVRWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLADWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_003585989.4
Organism	Bos taurus(cattle)
Definition	Bos taurus E3 ubiquitin-protein ligase HECW1 (LOC514243), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585989.4

ORF Insert Sequence:

ATGCGGAGAT CTGGATCCAT CCAGCAGATG GAGCAGCTCA ACAGGCGGTA CCAAAACATT 
CAGCGAACCA TTGCTACAGA GAGGCCTGAA GAAGATTCTG GTAGCCAAAT TTGTGATCCA 
GTTCCAGCAG GAGGCGGTGG AGGAGGTGGG AGTGACTCAG AGGCTGAATC TTCGAGCTTA 
GATCTGAGAA GAGATGGGTC ACTTTCTCCA GTGAACTCAC AAAAAATCAC CTTGCTGCTA 
CAGTCTCCAG CGGTCAAATT CATCACCAAC CCTGAGTTCT TCACTGTACT GCATGCCAAC 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTGA AGCACATGAT CCTCAAAGTC 
CGGCGGGATG CGCGCAACTT CGAACGCTAC CAGCACAACC GAGACTTGGT GAATTTCATC 
AACATGTTCG CGGACACGCG GCTGGAATTG CCCCGGGGCT GGGAGATCAA AACGGACCAG 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CCACTTTTAT TGACCCCCGA 
ATCCCTCTTC AGAACGGTCG TCTTCCCAAC CATCTGACTC ATCGACAGCA TCTCCAGAGG 
CTCCGAAGTT ACAGTGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCTTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGACTCA 
TTGCCACTGG CATATAACGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA TATTTTTGAA 
ATGCTGCAAG AGCGCCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TACATTCGGA CTGAGGGTAA TCATGGGCTT GAAAAGTTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA GGAAGAGATT ATGTCCTATG TCCCCCTGCA GGCCGCCTTC 
CACCCTGGGT ACAGCTTCTC TCCCCGCTGC TCACCCTGCT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCCTACA GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATCA TTCGTCGAGA CCACCTGTTG GAGGGAACCT TCAATCAGGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGCG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCGAG AGAATTCTTC TTCCTTCTGT CTCAGGAGCT CTTTAACCCT 
TACTACGGAC TCTTCGAGTA CTCTGCCAAT GACACCTACA CCGTGCAGAT CAGCCCCATG 
TCTGCCTTTG TAGAAAACCA TCTTGAATGG TTCAGGTTCA GTGGTCGAAT CCTAGGCCTG 
GCTCTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GACCCTTCTA TAAGGCGCTC 
CTGAGACTAC CCTGTGATTT GAGTGACCTG GAATATCTAG ATGAGGAATT CCACCAGAGC 
TTGCAATGGA TGAAGGACAA CAATATCACA GACATCCTCG ACCTCACGTT CACGGTTAAT 
GAAGAGGTTT TTGGCCAGGT AACGGAGAGG GAGTTAAAAT CTGGAGGTGC CAACACACAG 
GTGACAGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TGAGGTGGCG GGTAGAGCGC 
GGCGTGGTGC AGCAGACCGA GGCACTGGTG CGGGGCTTCT ACGAGGTGGT GGACTCAAGG 
CTGGTCTCCG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TCGCTGGCAC AGCAGAGATT 
GACCTGGCCG ACTGGCGGAA CAACACTGAG TACCGGGGAG GTTATCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCGGTGGAG CGTTTCAACA ATGAGCAGAG ACTCAGATTA 
CTGCAGTTCG TCACAGGAAC ATCAAGCGTG CCCTACGAAG GCTTTGCAGC CCTGCGAGGA 
AGCAACGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACATC TCTTCCCAGG 
GCACACACGT GCTTCAATCG ATTGGATCTT CCACCTTATC CCTCGTACTC CATGTTGTAT 
GAAAAGCTGT TAACGGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015463079.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318565.1 Bos taurus E3 ubiquitin-protein ligase HECW1 (LOC514243), mRNA.

Price & Availability↑

CloneID	OBa48879
Clone ID Related Accession (Same CDS sequence)	XM_003585989.4 , XM_015463079.1
Accession Version	XM_015463079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGAT CTGGATCCAT CCAGCAGATG GAGCAGCTCA ACAGGCGGTA CCAAAACATT 
CAGCGAACCA TTGCTACAGA GAGGCCTGAA GAAGATTCTG GTAGCCAAAT TTGTGATCCA 
GTTCCAGCAG GAGGCGGTGG AGGAGGTGGG AGTGACTCAG AGGCTGAATC TTCGAGCTTA 
GATCTGAGAA GAGATGGGTC ACTTTCTCCA GTGAACTCAC AAAAAATCAC CTTGCTGCTA 
CAGTCTCCAG CGGTCAAATT CATCACCAAC CCTGAGTTCT TCACTGTACT GCATGCCAAC 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTGA AGCACATGAT CCTCAAAGTC 
CGGCGGGATG CGCGCAACTT CGAACGCTAC CAGCACAACC GAGACTTGGT GAATTTCATC 
AACATGTTCG CGGACACGCG GCTGGAATTG CCCCGGGGCT GGGAGATCAA AACGGACCAG 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CCACTTTTAT TGACCCCCGA 
ATCCCTCTTC AGAACGGTCG TCTTCCCAAC CATCTGACTC ATCGACAGCA TCTCCAGAGG 
CTCCGAAGTT ACAGTGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCTTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGACTCA 
TTGCCACTGG CATATAACGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA TATTTTTGAA 
ATGCTGCAAG AGCGCCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TACATTCGGA CTGAGGGTAA TCATGGGCTT GAAAAGTTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA GGAAGAGATT ATGTCCTATG TCCCCCTGCA GGCCGCCTTC 
CACCCTGGGT ACAGCTTCTC TCCCCGCTGC TCACCCTGCT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCCTACA GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATCA TTCGTCGAGA CCACCTGTTG GAGGGAACCT TCAATCAGGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGCG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCGAG AGAATTCTTC TTCCTTCTGT CTCAGGAGCT CTTTAACCCT 
TACTACGGAC TCTTCGAGTA CTCTGCCAAT GACACCTACA CCGTGCAGAT CAGCCCCATG 
TCTGCCTTTG TAGAAAACCA TCTTGAATGG TTCAGGTTCA GTGGTCGAAT CCTAGGCCTG 
GCTCTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GACCCTTCTA TAAGGCGCTC 
CTGAGACTAC CCTGTGATTT GAGTGACCTG GAATATCTAG ATGAGGAATT CCACCAGAGC 
TTGCAATGGA TGAAGGACAA CAATATCACA GACATCCTCG ACCTCACGTT CACGGTTAAT 
GAAGAGGTTT TTGGCCAGGT AACGGAGAGG GAGTTAAAAT CTGGAGGTGC CAACACACAG 
GTGACAGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TGAGGTGGCG GGTAGAGCGC 
GGCGTGGTGC AGCAGACCGA GGCACTGGTG CGGGGCTTCT ACGAGGTGGT GGACTCAAGG 
CTGGTCTCCG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TCGCTGGCAC AGCAGAGATT 
GACCTGGCCG ACTGGCGGAA CAACACTGAG TACCGGGGAG GTTATCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCGGTGGAG CGTTTCAACA ATGAGCAGAG ACTCAGATTA 
CTGCAGTTCG TCACAGGAAC ATCAAGCGTG CCCTACGAAG GCTTTGCAGC CCTGCGAGGA 
AGCAACGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACATC TCTTCCCAGG 
GCACACACGT GCTTCAATCG ATTGGATCTT CCACCTTATC CCTCGTACTC CATGTTGTAT 
GAAAAGCTGT TAACGGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318565.1
CDS	802..3012
Translation	MRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGSQICDPVPAGGGGGGGSDSEAESSSLDLRRDGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHDSLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVRWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLADWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015463079.1
Organism	Bos taurus(cattle)
Definition	Bos taurus E3 ubiquitin-protein ligase HECW1 (LOC514243), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463079.1

ORF Insert Sequence:

ATGCGGAGAT CTGGATCCAT CCAGCAGATG GAGCAGCTCA ACAGGCGGTA CCAAAACATT 
CAGCGAACCA TTGCTACAGA GAGGCCTGAA GAAGATTCTG GTAGCCAAAT TTGTGATCCA 
GTTCCAGCAG GAGGCGGTGG AGGAGGTGGG AGTGACTCAG AGGCTGAATC TTCGAGCTTA 
GATCTGAGAA GAGATGGGTC ACTTTCTCCA GTGAACTCAC AAAAAATCAC CTTGCTGCTA 
CAGTCTCCAG CGGTCAAATT CATCACCAAC CCTGAGTTCT TCACTGTACT GCATGCCAAC 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTGA AGCACATGAT CCTCAAAGTC 
CGGCGGGATG CGCGCAACTT CGAACGCTAC CAGCACAACC GAGACTTGGT GAATTTCATC 
AACATGTTCG CGGACACGCG GCTGGAATTG CCCCGGGGCT GGGAGATCAA AACGGACCAG 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CCACTTTTAT TGACCCCCGA 
ATCCCTCTTC AGAACGGTCG TCTTCCCAAC CATCTGACTC ATCGACAGCA TCTCCAGAGG 
CTCCGAAGTT ACAGTGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCTTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGACTCA 
TTGCCACTGG CATATAACGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA TATTTTTGAA 
ATGCTGCAAG AGCGCCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TACATTCGGA CTGAGGGTAA TCATGGGCTT GAAAAGTTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA GGAAGAGATT ATGTCCTATG TCCCCCTGCA GGCCGCCTTC 
CACCCTGGGT ACAGCTTCTC TCCCCGCTGC TCACCCTGCT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCCTACA GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATCA TTCGTCGAGA CCACCTGTTG GAGGGAACCT TCAATCAGGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGCG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCGAG AGAATTCTTC TTCCTTCTGT CTCAGGAGCT CTTTAACCCT 
TACTACGGAC TCTTCGAGTA CTCTGCCAAT GACACCTACA CCGTGCAGAT CAGCCCCATG 
TCTGCCTTTG TAGAAAACCA TCTTGAATGG TTCAGGTTCA GTGGTCGAAT CCTAGGCCTG 
GCTCTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GACCCTTCTA TAAGGCGCTC 
CTGAGACTAC CCTGTGATTT GAGTGACCTG GAATATCTAG ATGAGGAATT CCACCAGAGC 
TTGCAATGGA TGAAGGACAA CAATATCACA GACATCCTCG ACCTCACGTT CACGGTTAAT 
GAAGAGGTTT TTGGCCAGGT AACGGAGAGG GAGTTAAAAT CTGGAGGTGC CAACACACAG 
GTGACAGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TGAGGTGGCG GGTAGAGCGC 
GGCGTGGTGC AGCAGACCGA GGCACTGGTG CGGGGCTTCT ACGAGGTGGT GGACTCAAGG 
CTGGTCTCCG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TCGCTGGCAC AGCAGAGATT 
GACCTGGCCG ACTGGCGGAA CAACACTGAG TACCGGGGAG GTTATCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCGGTGGAG CGTTTCAACA ATGAGCAGAG ACTCAGATTA 
CTGCAGTTCG TCACAGGAAC ATCAAGCGTG CCCTACGAAG GCTTTGCAGC CCTGCGAGGA 
AGCAACGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACATC TCTTCCCAGG 
GCACACACGT GCTTCAATCG ATTGGATCTT CCACCTTATC CCTCGTACTC CATGTTGTAT 
GAAAAGCTGT TAACGGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_003585989.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586037.4 Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa234009
Clone ID Related Accession (Same CDS sequence)	XM_003585989.5
Accession Version	XM_003585989.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003585989.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC CGAAAAGGCC AGCCTTTAAA GACCCCGGCA GTGTCGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC CTCAGCGGAC TCGCTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTCTTAGGC CTGGCCGCCA TGGCATCTCC ATCTAGAAAC 
TCCCAGAATC GACGCCGGTG CAAGGAGCCG CTCCGCCACA GCTACAATCC AGGCCAGTAC 
CACAGTGTGG CCATCAGGAC TGGCCCCCAT GGTGCCGTCA CCATCCCCCG CTCCACCAGT 
GACACCGACC TGGTCACCTC GGACAGCCGC TCCACACTCA TGGTCAGCAG TTCCTACTAC 
TCCATAGGAC ACTCGCAGGA CCTGGTGATC CACTGGGACA TCAAGGAGGA AGTGGATGCT 
GGGGACTGGA TCGGCATGTA CCTCATTGAT GAGGTCTTGT CGGAAAACTT CCTGGACTAT 
AAGAACCGTG GAGTCAATGG CTCTCATCGG GGCCAGATCA TTTGGAAGAT TGATGCCAGC 
TCTTACTTTG TAGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTAAGTGGA 
GCCTTGCGAG CCACCACCCC CAGTGTCACC GTCAAAAACT CAGCAGCTCC TATTTTTAAA 
AGCATTGGCT CTGATGAGAC CGTGCAAGGG CAAGGAAGTC GGAGGCTGAT CAGCTTTTCT 
CTCTCAGATT TCCAGGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAC 
CTGAAGATTT CTATTCAGCC TGGGAAGCAC AGCATCTTTC CTGCCCTCCC CCACCATGGA 
CAGGAGAGGC GATCCAAAAT CATTGGCAAC ACTGTGAACC CCATCTGGCA GGCTGAGCAA 
TTCAGCTTTG TGTCCTTGCC CACTGATGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTTTTG GGAAAGCTGT CAATGCCCGT TCAAAGACTC 
CTGGAAAGAC ATGCCATAGG GGACAGGGTG GTCAGCTACA CCCTTGGCCG TAGGCTTCCA 
ACAGATCACG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GATGATGAGG AGATTTCCCT GAGTACCGAG CCTGAGTCTG CGGAAATTCA GGACAGCCCC 
ATGAATAACC TGGTGGCAAG CAGCTGTGGG GAGCCCCCGT GTGATGCACC AGAGCCCTCA 
GGGACCCCAA AGCCTGAGCA GCCGAGCCAG GGCAGCACTG TGGGCCCTCC AGAGATCGAG 
GGCCTGGAGC TTACCAGTCT GGTTGAGGCA GCGGCAGGTA CCACGGAGGC AGGAGATGAT 
GATGGCATAG TCTCTGTGGG GCCTGAGGGG GCTGGGGAGC TGCTGGCCCC TGGGCCAGAG 
GACACAGAGC CCGCCCTAAG TGCAGAAGAA CTGGCCGAGC GGCTGGACCT GGGAGGGGAG 
GTGGCCCTCC CGGGGATGCT GGAGGACGGT CAAGCCCCTA GCAGCCCTGG GGAGCCGGTG 
GCTGAGGAAA GGGCCACAGA GAGTCGGGCT GGGGGACAGG AGGAGGAGAA GGAGCAGATG 
GAGGAGGAAG AGGGGGACGT GTCTGCCCTG GGGCAGGGGG AGGACAGGCT GCACCTGCGG 
GCCTCGGTGA AGAGGAAAAC CAGGCCGTGC TCCCTGCCTG TGTCCGAGCT GGAGACGGTG 
ATCGCGTCCG CTTGCGGTGA GCCGGAGACG CCGCGGACCC ACTACATCCG CATCCACACG 
CTGCTGCACA GCCTGCCCTC GGCGCAGGGC GGCAGCGCGG ACGACGACGA CGACGGTGCC 
GAGGAGGAGG CCACCCTCAA GGATGCCTCG GAGAAGGACG CGCTCAGTGA GATGGACACG 
GTCGCCGCGG AGCCTCCTCC CCTGGAAGAG GACGGAGAGG CCCCCGAGGG GGCCACTCCA 
GGCACGGCTC CCCTCGGCCA CTCGGGCTTG GCCAACGGCT CAGCCCCGGA TGGCGACGCG 
CTCCCCAGCA CGGGGAGCGA GAGCGACTCC AGCCCGCGGC AGGGTGGAGA CCACAGCTGC 
TGCGACGCAT CCTGCTGCAG CCCCTCCTGT TACAGCTCCT CGTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA GTAGGTTTGC CAGCCACACC 
CGCTTCTCCT CTGTGGACAG TGCCAAGATC TCCGAGAGCA CGGTCTTCTC CTCGCAGGAC 
GACGAGGAGG AGGAGAACAG CGCCTTCGAG TCAGTGCCCG ACTCAGTGCA GAGCCCAGAG 
CTGGACCAGG AGTCGGCCAA CGGTGCAGGG CCATGGCAAG ACGAGCTGGC CGCCCCCGAT 
GGGAGCATGG CCAGAACCAT GGAAGGTCTG GAGTCCCCCG TGGCAGGGCC GAGCAGTCGG 
AGAGAAGGTG AATGTCCTAT ACTCCATAAT TCCCAGCCAG TAAGCCAGCT TCCTTCCTTG 
AGGCCTGAAC ATCATCACTA CCCAACAATC GATGAGCCTC TTCCACCAAA CTGGGAAGCT 
CGCATTGACA GCCACGGGCG GGTCTTTTAT GTGGACCACG TGAACCGCAC AACCACCTGG 
CAGCGTCCCA CAGCAGCAGC CACCCCCGAC GGGATGCGGA GATCTGGATC CATCCAGCAG 
ATGGAGCAGC TCAACAGGCG GTACCAAAAC ATTCAGCGAA CCATTGCTAC AGAGAGGCCT 
GAAGAAGATT CTGGTAGCCA AATTTGTGAT CCAGTTCCAG CAGGAGGCGG TGGAGGAGGT 
GGGAGTGACT CAGAGGCTGA ATCTTCGAGC TTAGATCTGA GAAGAGATGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTACAGTCTC CAGCGGTCAA ATTCATCACC 
AACCCTGAGT TCTTCACTGT ACTGCATGCC AACTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TGAAGCACAT GATCCTCAAA GTCCGGCGGG ATGCGCGCAA CTTCGAACGC 
TACCAGCACA ACCGAGACTT GGTGAATTTC ATCAACATGT TCGCGGACAC GCGGCTGGAA 
TTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTCTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT TATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AACCATCTGA CTCATCGACA GCATCTCCAG AGGCTCCGAA GTTACAGTGC TGGAGAGGCC 
TCAGAAGTCT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCCTAGTA 
GCTGCTATTC GAAGCCAACA CCAACATGAC TCATTGCCAC TGGCATATAA CGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAATATTTTT GAAATGCTGC AAGAGCGCCA GCCAAGCTTG 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCATGGG 
CTTGAAAAGT TGTCCTGTGA TGCAGATCTA GTCATTTTGC TGAGTCTCTT TGAGGAAGAG 
ATTATGTCCT ATGTCCCCCT GCAGGCCGCC TTCCACCCTG GGTACAGCTT CTCTCCCCGC 
TGCTCACCCT GCTCCTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCACCCT ACAGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACCG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TCATTCGTCG AGACCACCTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCCCGGA AGGAGCTCCA GCGAAACAAG 
CTCTACATCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGTCCTTC GAGAGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTTAAC CCTTACTACG GACTCTTCGA GTACTCTGCC 
AATGACACCT ACACCGTGCA GATCAGCCCC ATGTCTGCCT TTGTAGAAAA CCATCTTGAA 
TGGTTCAGGT TCAGTGGTCG AATCCTAGGC CTGGCTCTGA TCCATCAGTA CCTTCTGGAT 
GCTTTCTTCA CAAGACCCTT CTATAAGGCG CTCCTGAGAC TACCCTGTGA TTTGAGTGAC 
CTGGAATATC TAGATGAGGA ATTCCACCAG AGCTTGCAAT GGATGAAGGA CAACAATATC 
ACAGACATCC TCGACCTCAC GTTCACGGTT AATGAAGAGG TTTTTGGCCA GGTAACGGAG 
AGGGAGTTAA AATCTGGAGG TGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGGA TGGTGAGGTG GCGGGTAGAG CGCGGCGTGG TGCAGCAGAC CGAGGCACTG 
GTGCGGGGCT TCTACGAGGT GGTGGACTCA AGGCTGGTCT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATCGCTGG CACAGCAGAG ATTGACCTGG CCGACTGGCG GAACAACACT 
GAGTACCGGG GAGGTTATCA TGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGTTTCA ACAATGAGCA GAGACTCAGA TTACTGCAGT TCGTCACAGG AACATCAAGC 
GTGCCCTACG AAGGCTTTGC AGCCCTGCGA GGAAGCAACG GGCTTCGGCG TTTCTGCATA 
GAGAAATGGG GGAAAATTAC ATCTCTTCCC AGGGCACACA CGTGCTTCAA TCGATTGGAT 
CTTCCACCTT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACGGC AGTAGAAGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586037.4
CDS	1151..6034
Translation	MPYPKRPAFKDPGSVVVQGVSKQVARSAFLLSGLAPAVAITNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQYHSVAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGSQICDPVPAGGGGGGGSDSEAESSSLDLRRDGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHDSLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVRWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLADWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_003585989.5
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003585989.5

ORF Insert Sequence:

ATGCCCTACC CGAAAAGGCC AGCCTTTAAA GACCCCGGCA GTGTCGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC CTCAGCGGAC TCGCTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTCTTAGGC CTGGCCGCCA TGGCATCTCC ATCTAGAAAC 
TCCCAGAATC GACGCCGGTG CAAGGAGCCG CTCCGCCACA GCTACAATCC AGGCCAGTAC 
CACAGTGTGG CCATCAGGAC TGGCCCCCAT GGTGCCGTCA CCATCCCCCG CTCCACCAGT 
GACACCGACC TGGTCACCTC GGACAGCCGC TCCACACTCA TGGTCAGCAG TTCCTACTAC 
TCCATAGGAC ACTCGCAGGA CCTGGTGATC CACTGGGACA TCAAGGAGGA AGTGGATGCT 
GGGGACTGGA TCGGCATGTA CCTCATTGAT GAGGTCTTGT CGGAAAACTT CCTGGACTAT 
AAGAACCGTG GAGTCAATGG CTCTCATCGG GGCCAGATCA TTTGGAAGAT TGATGCCAGC 
TCTTACTTTG TAGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTAAGTGGA 
GCCTTGCGAG CCACCACCCC CAGTGTCACC GTCAAAAACT CAGCAGCTCC TATTTTTAAA 
AGCATTGGCT CTGATGAGAC CGTGCAAGGG CAAGGAAGTC GGAGGCTGAT CAGCTTTTCT 
CTCTCAGATT TCCAGGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAC 
CTGAAGATTT CTATTCAGCC TGGGAAGCAC AGCATCTTTC CTGCCCTCCC CCACCATGGA 
CAGGAGAGGC GATCCAAAAT CATTGGCAAC ACTGTGAACC CCATCTGGCA GGCTGAGCAA 
TTCAGCTTTG TGTCCTTGCC CACTGATGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTTTTG GGAAAGCTGT CAATGCCCGT TCAAAGACTC 
CTGGAAAGAC ATGCCATAGG GGACAGGGTG GTCAGCTACA CCCTTGGCCG TAGGCTTCCA 
ACAGATCACG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GATGATGAGG AGATTTCCCT GAGTACCGAG CCTGAGTCTG CGGAAATTCA GGACAGCCCC 
ATGAATAACC TGGTGGCAAG CAGCTGTGGG GAGCCCCCGT GTGATGCACC AGAGCCCTCA 
GGGACCCCAA AGCCTGAGCA GCCGAGCCAG GGCAGCACTG TGGGCCCTCC AGAGATCGAG 
GGCCTGGAGC TTACCAGTCT GGTTGAGGCA GCGGCAGGTA CCACGGAGGC AGGAGATGAT 
GATGGCATAG TCTCTGTGGG GCCTGAGGGG GCTGGGGAGC TGCTGGCCCC TGGGCCAGAG 
GACACAGAGC CCGCCCTAAG TGCAGAAGAA CTGGCCGAGC GGCTGGACCT GGGAGGGGAG 
GTGGCCCTCC CGGGGATGCT GGAGGACGGT CAAGCCCCTA GCAGCCCTGG GGAGCCGGTG 
GCTGAGGAAA GGGCCACAGA GAGTCGGGCT GGGGGACAGG AGGAGGAGAA GGAGCAGATG 
GAGGAGGAAG AGGGGGACGT GTCTGCCCTG GGGCAGGGGG AGGACAGGCT GCACCTGCGG 
GCCTCGGTGA AGAGGAAAAC CAGGCCGTGC TCCCTGCCTG TGTCCGAGCT GGAGACGGTG 
ATCGCGTCCG CTTGCGGTGA GCCGGAGACG CCGCGGACCC ACTACATCCG CATCCACACG 
CTGCTGCACA GCCTGCCCTC GGCGCAGGGC GGCAGCGCGG ACGACGACGA CGACGGTGCC 
GAGGAGGAGG CCACCCTCAA GGATGCCTCG GAGAAGGACG CGCTCAGTGA GATGGACACG 
GTCGCCGCGG AGCCTCCTCC CCTGGAAGAG GACGGAGAGG CCCCCGAGGG GGCCACTCCA 
GGCACGGCTC CCCTCGGCCA CTCGGGCTTG GCCAACGGCT CAGCCCCGGA TGGCGACGCG 
CTCCCCAGCA CGGGGAGCGA GAGCGACTCC AGCCCGCGGC AGGGTGGAGA CCACAGCTGC 
TGCGACGCAT CCTGCTGCAG CCCCTCCTGT TACAGCTCCT CGTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA GTAGGTTTGC CAGCCACACC 
CGCTTCTCCT CTGTGGACAG TGCCAAGATC TCCGAGAGCA CGGTCTTCTC CTCGCAGGAC 
GACGAGGAGG AGGAGAACAG CGCCTTCGAG TCAGTGCCCG ACTCAGTGCA GAGCCCAGAG 
CTGGACCAGG AGTCGGCCAA CGGTGCAGGG CCATGGCAAG ACGAGCTGGC CGCCCCCGAT 
GGGAGCATGG CCAGAACCAT GGAAGGTCTG GAGTCCCCCG TGGCAGGGCC GAGCAGTCGG 
AGAGAAGGTG AATGTCCTAT ACTCCATAAT TCCCAGCCAG TAAGCCAGCT TCCTTCCTTG 
AGGCCTGAAC ATCATCACTA CCCAACAATC GATGAGCCTC TTCCACCAAA CTGGGAAGCT 
CGCATTGACA GCCACGGGCG GGTCTTTTAT GTGGACCACG TGAACCGCAC AACCACCTGG 
CAGCGTCCCA CAGCAGCAGC CACCCCCGAC GGGATGCGGA GATCTGGATC CATCCAGCAG 
ATGGAGCAGC TCAACAGGCG GTACCAAAAC ATTCAGCGAA CCATTGCTAC AGAGAGGCCT 
GAAGAAGATT CTGGTAGCCA AATTTGTGAT CCAGTTCCAG CAGGAGGCGG TGGAGGAGGT 
GGGAGTGACT CAGAGGCTGA ATCTTCGAGC TTAGATCTGA GAAGAGATGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTACAGTCTC CAGCGGTCAA ATTCATCACC 
AACCCTGAGT TCTTCACTGT ACTGCATGCC AACTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TGAAGCACAT GATCCTCAAA GTCCGGCGGG ATGCGCGCAA CTTCGAACGC 
TACCAGCACA ACCGAGACTT GGTGAATTTC ATCAACATGT TCGCGGACAC GCGGCTGGAA 
TTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTCTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT TATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AACCATCTGA CTCATCGACA GCATCTCCAG AGGCTCCGAA GTTACAGTGC TGGAGAGGCC 
TCAGAAGTCT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCCTAGTA 
GCTGCTATTC GAAGCCAACA CCAACATGAC TCATTGCCAC TGGCATATAA CGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAATATTTTT GAAATGCTGC AAGAGCGCCA GCCAAGCTTG 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCATGGG 
CTTGAAAAGT TGTCCTGTGA TGCAGATCTA GTCATTTTGC TGAGTCTCTT TGAGGAAGAG 
ATTATGTCCT ATGTCCCCCT GCAGGCCGCC TTCCACCCTG GGTACAGCTT CTCTCCCCGC 
TGCTCACCCT GCTCCTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCACCCT ACAGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACCG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TCATTCGTCG AGACCACCTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCCCGGA AGGAGCTCCA GCGAAACAAG 
CTCTACATCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGTCCTTC GAGAGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTTAAC CCTTACTACG GACTCTTCGA GTACTCTGCC 
AATGACACCT ACACCGTGCA GATCAGCCCC ATGTCTGCCT TTGTAGAAAA CCATCTTGAA 
TGGTTCAGGT TCAGTGGTCG AATCCTAGGC CTGGCTCTGA TCCATCAGTA CCTTCTGGAT 
GCTTTCTTCA CAAGACCCTT CTATAAGGCG CTCCTGAGAC TACCCTGTGA TTTGAGTGAC 
CTGGAATATC TAGATGAGGA ATTCCACCAG AGCTTGCAAT GGATGAAGGA CAACAATATC 
ACAGACATCC TCGACCTCAC GTTCACGGTT AATGAAGAGG TTTTTGGCCA GGTAACGGAG 
AGGGAGTTAA AATCTGGAGG TGCCAACACA CAGGTGACAG AGAAGAACAA GAAGGAGTAC 
ATCGAGAGGA TGGTGAGGTG GCGGGTAGAG CGCGGCGTGG TGCAGCAGAC CGAGGCACTG 
GTGCGGGGCT TCTACGAGGT GGTGGACTCA AGGCTGGTCT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATCGCTGG CACAGCAGAG ATTGACCTGG CCGACTGGCG GAACAACACT 
GAGTACCGGG GAGGTTATCA TGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGTTTCA ACAATGAGCA GAGACTCAGA TTACTGCAGT TCGTCACAGG AACATCAAGC 
GTGCCCTACG AAGGCTTTGC AGCCCTGCGA GGAAGCAACG GGCTTCGGCG TTTCTGCATA 
GAGAAATGGG GGAAAATTAC ATCTCTTCCC AGGGCACACA CGTGCTTCAA TCGATTGGAT 
CTTCCACCTT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACGGC AGTAGAAGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_024991127.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846895.1 Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

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CloneID	OBa234010
Clone ID Related Accession (Same CDS sequence)	XM_024991127.1
Accession Version	XM_024991127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCGG AGCTGCCAAG AATCTGTACC AGAACAGGTT CTTAGGCCTG 
GCCGCCATGG CATCTCCATC TAGAAACTCC CAGAATCGAC GCCGGTGCAA GGAGCCGCTC 
CGCCACAGCT ACAATCCAGG CCAGTACCAC AGTGTGGCCA TCAGGACTGG CCCCCATGGT 
GCCGTCACCA TCCCCCGCTC CACCAGTGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACACTCATGG TCAGCAGTTC CTACTACTCC ATAGGACACT CGCAGGACCT GGTGATCCAC 
TGGGACATCA AGGAGGAAGT GGATGCTGGG GACTGGATCG GCATGTACCT CATTGATGAG 
GTCTTGTCGG AAAACTTCCT GGACTATAAG AACCGTGGAG TCAATGGCTC TCATCGGGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTAG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT AAGTGGAGCC TTGCGAGCCA CCACCCCCAG TGTCACCGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATTGGCTCTG ATGAGACCGT GCAAGGGCAA 
GGAAGTCGGA GGCTGATCAG CTTTTCTCTC TCAGATTTCC AGGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTACCTG AAGATTTCTA TTCAGCCTGG GAAGCACAGC 
ATCTTTCCTG CCCTCCCCCA CCATGGACAG GAGAGGCGAT CCAAAATCAT TGGCAACACT 
GTGAACCCCA TCTGGCAGGC TGAGCAATTC AGCTTTGTGT CCTTGCCCAC TGATGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTTTTGGGA 
AAGCTGTCAA TGCCCGTTCA AAGACTCCTG GAAAGACATG CCATAGGGGA CAGGGTGGTC 
AGCTACACCC TTGGCCGTAG GCTTCCAACA GATCACGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCTGCGG AAATTCAGGA CAGCCCCATG AATAACCTGG TGGCAAGCAG CTGTGGGGAG 
CCCCCGTGTG ATGCACCAGA GCCCTCAGGG ACCCCAAAGC CTGAGCAGCC GAGCCAGGGC 
AGCACTGTGG GCCCTCCAGA GATCGAGGGC CTGGAGCTTA CCAGTCTGGT TGAGGCAGCG 
GCAGGTACCA CGGAGGCAGG AGATGATGAT GGCATAGTCT CTGTGGGGCC TGAGGGGGCT 
GGGGAGCTGC TGGCCCCTGG GCCAGAGGAC ACAGAGCCCG CCCTAAGTGC AGAAGAACTG 
GCCGAGCGGC TGGACCTGGG AGGGGAGGTG GCCCTCCCGG GGATGCTGGA GGACGGTCAA 
GCCCCTAGCA GCCCTGGGGA GCCGGTGGCT GAGGAAAGGG CCACAGAGAG TCGGGCTGGG 
GGACAGGAGG AGGAGAAGGA GCAGATGGAG GAGGAAGAGG GGGACGTGTC TGCCCTGGGG 
CAGGGGGAGG ACAGGCTGCA CCTGCGGGCC TCGGTGAAGA GGAAAACCAG GCCGTGCTCC 
CTGCCTGTGT CCGAGCTGGA GACGGTGATC GCGTCCGCTT GCGGTGAGCC GGAGACGCCG 
CGGACCCACT ACATCCGCAT CCACACGCTG CTGCACAGCC TGCCCTCGGC GCAGGGCGGC 
AGCGCGGACG ACGACGACGA CGGTGCCGAG GAGGAGGCCA CCCTCAAGGA TGCCTCGGAG 
AAGGACGCGC TCAGTGAGAT GGACACGGTC GCCGCGGAGC CTCCTCCCCT GGAAGAGGAC 
GGAGAGGCCC CCGAGGGGGC CACTCCAGGC ACGGCTCCCC TCGGCCACTC GGGCTTGGCC 
AACGGCTCAG CCCCGGATGG CGACGCGCTC CCCAGCACGG GGAGCGAGAG CGACTCCAGC 
CCGCGGCAGG GTGGAGACCA CAGCTGCTGC GACGCATCCT GCTGCAGCCC CTCCTGTTAC 
AGCTCCTCGT GCTACAGCAG CTCCTGCTAC AGCGCCTCCT GCTACAGCCC CTCCTGCTAC 
AACGGCAGTA GGTTTGCCAG CCACACCCGC TTCTCCTCTG TGGACAGTGC CAAGATCTCC 
GAGAGCACGG TCTTCTCCTC GCAGGACGAC GAGGAGGAGG AGAACAGCGC CTTCGAGTCA 
GTGCCCGACT CAGTGCAGAG CCCAGAGCTG GACCAGGAGT CGGCCAACGG TGCAGGGCCA 
TGGCAAGACG AGCTGGCCGC CCCCGATGGG AGCATGGCCA GAACCATGGA AGGTCTGGAG 
TCCCCCGTGG CAGGGCCGAG CAGTCGGAGA GAAGGTGAAT GTCCTATACT CCATAATTCC 
CAGCCAGTAA GCCAGCTTCC TTCCTTGAGG CCTGAACATC ATCACTACCC AACAATCGAT 
GAGCCTCTTC CACCAAACTG GGAAGCTCGC ATTGACAGCC ACGGGCGGGT CTTTTATGTG 
GACCACGTGA ACCGCACAAC CACCTGGCAG CGTCCCACAG CAGCAGCCAC CCCCGACGGG 
ATGCGGAGAT CTGGATCCAT CCAGCAGATG GAGCAGCTCA ACAGGCGGTA CCAAAACATT 
CAGCGAACCA TTGCTACAGA GAGGCCTGAA GAAGATTCTG GTAGCCAAAT TTGTGATCCA 
GTTCCAGCAG GAGGCGGTGG AGGAGGTGGG AGTGACTCAG AGGCTGAATC TTCGAGCTTA 
GATCTGAGAA GAGATGGGTC ACTTTCTCCA GTGAACTCAC AAAAAATCAC CTTGCTGCTA 
CAGTCTCCAG CGGTCAAATT CATCACCAAC CCTGAGTTCT TCACTGTACT GCATGCCAAC 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTGA AGCACATGAT CCTCAAAGTC 
CGGCGGGATG CGCGCAACTT CGAACGCTAC CAGCACAACC GAGACTTGGT GAATTTCATC 
AACATGTTCG CGGACACGCG GCTGGAATTG CCCCGGGGCT GGGAGATCAA AACGGACCAG 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CCACTTTTAT TGACCCCCGA 
ATCCCTCTTC AGAACGGTCG TCTTCCCAAC CATCTGACTC ATCGACAGCA TCTCCAGAGG 
CTCCGAAGTT ACAGTGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCTTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGACTCA 
TTGCCACTGG CATATAACGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA TATTTTTGAA 
ATGCTGCAAG AGCGCCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TACATTCGGA CTGAGGGTAA TCATGGGCTT GAAAAGTTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA GGAAGAGATT ATGTCCTATG TCCCCCTGCA GGCCGCCTTC 
CACCCTGGGT ACAGCTTCTC TCCCCGCTGC TCACCCTGCT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCCTACA GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATCA TTCGTCGAGA CCACCTGTTG GAGGGAACCT TCAATCAGGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGCG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCGAG AGAATTCTTC TTCCTTCTGT CTCAGGAGCT CTTTAACCCT 
TACTACGGAC TCTTCGAGTA CTCTGCCAAT GACACCTACA CCGTGCAGAT CAGCCCCATG 
TCTGCCTTTG TAGAAAACCA TCTTGAATGG TTCAGGTTCA GTGGTCGAAT CCTAGGCCTG 
GCTCTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GACCCTTCTA TAAGGCGCTC 
CTGAGACTAC CCTGTGATTT GAGTGACCTG GAATATCTAG ATGAGGAATT CCACCAGAGC 
TTGCAATGGA TGAAGGACAA CAATATCACA GACATCCTCG ACCTCACGTT CACGGTTAAT 
GAAGAGGTTT TTGGCCAGGT AACGGAGAGG GAGTTAAAAT CTGGAGGTGC CAACACACAG 
GTGACAGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TGAGGTGGCG GGTAGAGCGC 
GGCGTGGTGC AGCAGACCGA GGCACTGGTG CGGGGCTTCT ACGAGGTGGT GGACTCAAGG 
CTGGTCTCCG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TCGCTGGCAC AGCAGAGATT 
GACCTGGCCG ACTGGCGGAA CAACACTGAG TACCGGGGAG GTTATCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCGGTGGAG CGTTTCAACA ATGAGCAGAG ACTCAGATTA 
CTGCAGTTCG TCACAGGAAC ATCAAGCGTG CCCTACGAAG GCTTTGCAGC CCTGCGAGGA 
AGCAACGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACATC TCTTCCCAGG 
GCACACACGT GCTTCAATCG ATTGGATCTT CCACCTTATC CCTCGTACTC CATGTTGTAT 
GAAAAGCTGT TAACGGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846895.1
CDS	305..5095
Translation	MICAQAGAAKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQYHSVAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGSQICDPVPAGGGGGGGSDSEAESSSLDLRRDGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHDSLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVRWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLADWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_024991127.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991127.1

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCGG AGCTGCCAAG AATCTGTACC AGAACAGGTT CTTAGGCCTG 
GCCGCCATGG CATCTCCATC TAGAAACTCC CAGAATCGAC GCCGGTGCAA GGAGCCGCTC 
CGCCACAGCT ACAATCCAGG CCAGTACCAC AGTGTGGCCA TCAGGACTGG CCCCCATGGT 
GCCGTCACCA TCCCCCGCTC CACCAGTGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACACTCATGG TCAGCAGTTC CTACTACTCC ATAGGACACT CGCAGGACCT GGTGATCCAC 
TGGGACATCA AGGAGGAAGT GGATGCTGGG GACTGGATCG GCATGTACCT CATTGATGAG 
GTCTTGTCGG AAAACTTCCT GGACTATAAG AACCGTGGAG TCAATGGCTC TCATCGGGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTAG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT AAGTGGAGCC TTGCGAGCCA CCACCCCCAG TGTCACCGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATTGGCTCTG ATGAGACCGT GCAAGGGCAA 
GGAAGTCGGA GGCTGATCAG CTTTTCTCTC TCAGATTTCC AGGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTACCTG AAGATTTCTA TTCAGCCTGG GAAGCACAGC 
ATCTTTCCTG CCCTCCCCCA CCATGGACAG GAGAGGCGAT CCAAAATCAT TGGCAACACT 
GTGAACCCCA TCTGGCAGGC TGAGCAATTC AGCTTTGTGT CCTTGCCCAC TGATGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTTTTGGGA 
AAGCTGTCAA TGCCCGTTCA AAGACTCCTG GAAAGACATG CCATAGGGGA CAGGGTGGTC 
AGCTACACCC TTGGCCGTAG GCTTCCAACA GATCACGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCTGCGG AAATTCAGGA CAGCCCCATG AATAACCTGG TGGCAAGCAG CTGTGGGGAG 
CCCCCGTGTG ATGCACCAGA GCCCTCAGGG ACCCCAAAGC CTGAGCAGCC GAGCCAGGGC 
AGCACTGTGG GCCCTCCAGA GATCGAGGGC CTGGAGCTTA CCAGTCTGGT TGAGGCAGCG 
GCAGGTACCA CGGAGGCAGG AGATGATGAT GGCATAGTCT CTGTGGGGCC TGAGGGGGCT 
GGGGAGCTGC TGGCCCCTGG GCCAGAGGAC ACAGAGCCCG CCCTAAGTGC AGAAGAACTG 
GCCGAGCGGC TGGACCTGGG AGGGGAGGTG GCCCTCCCGG GGATGCTGGA GGACGGTCAA 
GCCCCTAGCA GCCCTGGGGA GCCGGTGGCT GAGGAAAGGG CCACAGAGAG TCGGGCTGGG 
GGACAGGAGG AGGAGAAGGA GCAGATGGAG GAGGAAGAGG GGGACGTGTC TGCCCTGGGG 
CAGGGGGAGG ACAGGCTGCA CCTGCGGGCC TCGGTGAAGA GGAAAACCAG GCCGTGCTCC 
CTGCCTGTGT CCGAGCTGGA GACGGTGATC GCGTCCGCTT GCGGTGAGCC GGAGACGCCG 
CGGACCCACT ACATCCGCAT CCACACGCTG CTGCACAGCC TGCCCTCGGC GCAGGGCGGC 
AGCGCGGACG ACGACGACGA CGGTGCCGAG GAGGAGGCCA CCCTCAAGGA TGCCTCGGAG 
AAGGACGCGC TCAGTGAGAT GGACACGGTC GCCGCGGAGC CTCCTCCCCT GGAAGAGGAC 
GGAGAGGCCC CCGAGGGGGC CACTCCAGGC ACGGCTCCCC TCGGCCACTC GGGCTTGGCC 
AACGGCTCAG CCCCGGATGG CGACGCGCTC CCCAGCACGG GGAGCGAGAG CGACTCCAGC 
CCGCGGCAGG GTGGAGACCA CAGCTGCTGC GACGCATCCT GCTGCAGCCC CTCCTGTTAC 
AGCTCCTCGT GCTACAGCAG CTCCTGCTAC AGCGCCTCCT GCTACAGCCC CTCCTGCTAC 
AACGGCAGTA GGTTTGCCAG CCACACCCGC TTCTCCTCTG TGGACAGTGC CAAGATCTCC 
GAGAGCACGG TCTTCTCCTC GCAGGACGAC GAGGAGGAGG AGAACAGCGC CTTCGAGTCA 
GTGCCCGACT CAGTGCAGAG CCCAGAGCTG GACCAGGAGT CGGCCAACGG TGCAGGGCCA 
TGGCAAGACG AGCTGGCCGC CCCCGATGGG AGCATGGCCA GAACCATGGA AGGTCTGGAG 
TCCCCCGTGG CAGGGCCGAG CAGTCGGAGA GAAGGTGAAT GTCCTATACT CCATAATTCC 
CAGCCAGTAA GCCAGCTTCC TTCCTTGAGG CCTGAACATC ATCACTACCC AACAATCGAT 
GAGCCTCTTC CACCAAACTG GGAAGCTCGC ATTGACAGCC ACGGGCGGGT CTTTTATGTG 
GACCACGTGA ACCGCACAAC CACCTGGCAG CGTCCCACAG CAGCAGCCAC CCCCGACGGG 
ATGCGGAGAT CTGGATCCAT CCAGCAGATG GAGCAGCTCA ACAGGCGGTA CCAAAACATT 
CAGCGAACCA TTGCTACAGA GAGGCCTGAA GAAGATTCTG GTAGCCAAAT TTGTGATCCA 
GTTCCAGCAG GAGGCGGTGG AGGAGGTGGG AGTGACTCAG AGGCTGAATC TTCGAGCTTA 
GATCTGAGAA GAGATGGGTC ACTTTCTCCA GTGAACTCAC AAAAAATCAC CTTGCTGCTA 
CAGTCTCCAG CGGTCAAATT CATCACCAAC CCTGAGTTCT TCACTGTACT GCATGCCAAC 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTGA AGCACATGAT CCTCAAAGTC 
CGGCGGGATG CGCGCAACTT CGAACGCTAC CAGCACAACC GAGACTTGGT GAATTTCATC 
AACATGTTCG CGGACACGCG GCTGGAATTG CCCCGGGGCT GGGAGATCAA AACGGACCAG 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CCACTTTTAT TGACCCCCGA 
ATCCCTCTTC AGAACGGTCG TCTTCCCAAC CATCTGACTC ATCGACAGCA TCTCCAGAGG 
CTCCGAAGTT ACAGTGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCTTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGACTCA 
TTGCCACTGG CATATAACGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA TATTTTTGAA 
ATGCTGCAAG AGCGCCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TACATTCGGA CTGAGGGTAA TCATGGGCTT GAAAAGTTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA GGAAGAGATT ATGTCCTATG TCCCCCTGCA GGCCGCCTTC 
CACCCTGGGT ACAGCTTCTC TCCCCGCTGC TCACCCTGCT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCCTACA GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATCA TTCGTCGAGA CCACCTGTTG GAGGGAACCT TCAATCAGGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGCG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCGAG AGAATTCTTC TTCCTTCTGT CTCAGGAGCT CTTTAACCCT 
TACTACGGAC TCTTCGAGTA CTCTGCCAAT GACACCTACA CCGTGCAGAT CAGCCCCATG 
TCTGCCTTTG TAGAAAACCA TCTTGAATGG TTCAGGTTCA GTGGTCGAAT CCTAGGCCTG 
GCTCTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GACCCTTCTA TAAGGCGCTC 
CTGAGACTAC CCTGTGATTT GAGTGACCTG GAATATCTAG ATGAGGAATT CCACCAGAGC 
TTGCAATGGA TGAAGGACAA CAATATCACA GACATCCTCG ACCTCACGTT CACGGTTAAT 
GAAGAGGTTT TTGGCCAGGT AACGGAGAGG GAGTTAAAAT CTGGAGGTGC CAACACACAG 
GTGACAGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TGAGGTGGCG GGTAGAGCGC 
GGCGTGGTGC AGCAGACCGA GGCACTGGTG CGGGGCTTCT ACGAGGTGGT GGACTCAAGG 
CTGGTCTCCG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TCGCTGGCAC AGCAGAGATT 
GACCTGGCCG ACTGGCGGAA CAACACTGAG TACCGGGGAG GTTATCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCGGTGGAG CGTTTCAACA ATGAGCAGAG ACTCAGATTA 
CTGCAGTTCG TCACAGGAAC ATCAAGCGTG CCCTACGAAG GCTTTGCAGC CCTGCGAGGA 
AGCAACGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACATC TCTTCCCAGG 
GCACACACGT GCTTCAATCG ATTGGATCTT CCACCTTATC CCTCGTACTC CATGTTGTAT 
GAAAAGCTGT TAACGGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_024991128.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846896.1 Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

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CloneID	OBa03947
Clone ID Related Accession (Same CDS sequence)	XM_024991128.1
Accession Version	XM_024991128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCTGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTGGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACGCCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCGCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GACGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGACGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCCAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGTTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGCATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCCACAGCAG CAGCCACCCC CGACGGGATG 
CGGAGATCTG GATCCATCCA GCAGATGGAG CAGCTCAACA GGCGGTACCA AAACATTCAG 
CGAACCATTG CTACAGAGAG GCCTGAAGAA GATTCTGGTA GCCAAATTTG TGATCCAGTT 
CCAGCAGGAG GCGGTGGAGG AGGTGGGAGT GACTCAGAGG CTGAATCTTC GAGCTTAGAT 
CTGAGAAGAG ATGGGTCACT TTCTCCAGTG AACTCACAAA AAATCACCTT GCTGCTACAG 
TCTCCAGCGG TCAAATTCAT CACCAACCCT GAGTTCTTCA CTGTACTGCA TGCCAACTAT 
AGTGCCTACC GAGTCTTCAC CAGTAGCACC TGCTTGAAGC ACATGATCCT CAAAGTCCGG 
CGGGATGCGC GCAACTTCGA ACGCTACCAG CACAACCGAG ACTTGGTGAA TTTCATCAAC 
ATGTTCGCGG ACACGCGGCT GGAATTGCCC CGGGGCTGGG AGATCAAAAC GGACCAGCAG 
GGAAAGTCTT TCTTCGTGGA CCACAACAGT CGAGCTACCA CTTTTATTGA CCCCCGAATC 
CCTCTTCAGA ACGGTCGTCT TCCCAACCAT CTGACTCATC GACAGCATCT CCAGAGGCTC 
CGAAGTTACA GTGCTGGAGA GGCCTCAGAA GTCTCTAGAA ACAGAGGAGC CTCTTTACTG 
GCCAGGCCAG GACACAGCCT AGTAGCTGCT ATTCGAAGCC AACACCAACA TGACTCATTG 
CCACTGGCAT ATAACGACAA GATTGTGGCA TTTCTTCGCC AGCCAAATAT TTTTGAAATG 
CTGCAAGAGC GCCAGCCAAG CTTGGCAAGA AACCACACAC TCAGGGAGAA AATCCATTAC 
ATTCGGACTG AGGGTAATCA TGGGCTTGAA AAGTTGTCCT GTGATGCAGA TCTAGTCATT 
TTGCTGAGTC TCTTTGAGGA AGAGATTATG TCCTATGTCC CCCTGCAGGC CGCCTTCCAC 
CCTGGGTACA GCTTCTCTCC CCGCTGCTCA CCCTGCTCCT CACCTCAGAA CTCCCCAGGT 
TTACAGAGAG CCAGTGCAAG AGCCCCTTCA CCCTACAGGA GAGACTTTGA GGCCAAGCTC 
CGCAATTTCT ACCGAAAACT GGAAGCCAAA GGATTTGGTC AGGGTCCAGG GAAAATTAAG 
CTCATCATTC GTCGAGACCA CCTGTTGGAG GGAACCTTCA ATCAGGTGAT GGCCTATTCC 
CGGAAGGAGC TCCAGCGAAA CAAGCTCTAC ATCACCTTTG TTGGAGAGGA GGGCCTGGAC 
TACAGTGGTC CTTCGAGAGA ATTCTTCTTC CTTCTGTCTC AGGAGCTCTT TAACCCTTAC 
TACGGACTCT TCGAGTACTC TGCCAATGAC ACCTACACCG TGCAGATCAG CCCCATGTCT 
GCCTTTGTAG AAAACCATCT TGAATGGTTC AGGTTCAGTG GTCGAATCCT AGGCCTGGCT 
CTGATCCATC AGTACCTTCT GGATGCTTTC TTCACAAGAC CCTTCTATAA GGCGCTCCTG 
AGACTACCCT GTGATTTGAG TGACCTGGAA TATCTAGATG AGGAATTCCA CCAGAGCTTG 
CAATGGATGA AGGACAACAA TATCACAGAC ATCCTCGACC TCACGTTCAC GGTTAATGAA 
GAGGTTTTTG GCCAGGTAAC GGAGAGGGAG TTAAAATCTG GAGGTGCCAA CACACAGGTG 
ACAGAGAAGA ACAAGAAGGA GTACATCGAG AGGATGGTGA GGTGGCGGGT AGAGCGCGGC 
GTGGTGCAGC AGACCGAGGC ACTGGTGCGG GGCTTCTACG AGGTGGTGGA CTCAAGGCTG 
GTCTCCGTGT TTGATGCCAG GGAGCTGGAG CTGGTGATCG CTGGCACAGC AGAGATTGAC 
CTGGCCGACT GGCGGAACAA CACTGAGTAC CGGGGAGGTT ATCATGATGG GCATCTTGTG 
ATCCGCTGGT TCTGGGCTGC GGTGGAGCGT TTCAACAATG AGCAGAGACT CAGATTACTG 
CAGTTCGTCA CAGGAACATC AAGCGTGCCC TACGAAGGCT TTGCAGCCCT GCGAGGAAGC 
AACGGGCTTC GGCGTTTCTG CATAGAGAAA TGGGGGAAAA TTACATCTCT TCCCAGGGCA 
CACACGTGCT TCAATCGATT GGATCTTCCA CCTTATCCCT CGTACTCCAT GTTGTATGAA 
AAGCTGTTAA CGGCAGTAGA AGAAACCAGC ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846896.1
CDS	230..5017
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQYHSVAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGSQICDPVPAGGGGGGGSDSEAESSSLDLRRDGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHDSLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVRWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLADWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_024991128.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991128.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCTGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTGGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACGCCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCGCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GACGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGACGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCCAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGTTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGCATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCCACAGCAG CAGCCACCCC CGACGGGATG 
CGGAGATCTG GATCCATCCA GCAGATGGAG CAGCTCAACA GGCGGTACCA AAACATTCAG 
CGAACCATTG CTACAGAGAG GCCTGAAGAA GATTCTGGTA GCCAAATTTG TGATCCAGTT 
CCAGCAGGAG GCGGTGGAGG AGGTGGGAGT GACTCAGAGG CTGAATCTTC GAGCTTAGAT 
CTGAGAAGAG ATGGGTCACT TTCTCCAGTG AACTCACAAA AAATCACCTT GCTGCTACAG 
TCTCCAGCGG TCAAATTCAT CACCAACCCT GAGTTCTTCA CTGTACTGCA TGCCAACTAT 
AGTGCCTACC GAGTCTTCAC CAGTAGCACC TGCTTGAAGC ACATGATCCT CAAAGTCCGG 
CGGGATGCGC GCAACTTCGA ACGCTACCAG CACAACCGAG ACTTGGTGAA TTTCATCAAC 
ATGTTCGCGG ACACGCGGCT GGAATTGCCC CGGGGCTGGG AGATCAAAAC GGACCAGCAG 
GGAAAGTCTT TCTTCGTGGA CCACAACAGT CGAGCTACCA CTTTTATTGA CCCCCGAATC 
CCTCTTCAGA ACGGTCGTCT TCCCAACCAT CTGACTCATC GACAGCATCT CCAGAGGCTC 
CGAAGTTACA GTGCTGGAGA GGCCTCAGAA GTCTCTAGAA ACAGAGGAGC CTCTTTACTG 
GCCAGGCCAG GACACAGCCT AGTAGCTGCT ATTCGAAGCC AACACCAACA TGACTCATTG 
CCACTGGCAT ATAACGACAA GATTGTGGCA TTTCTTCGCC AGCCAAATAT TTTTGAAATG 
CTGCAAGAGC GCCAGCCAAG CTTGGCAAGA AACCACACAC TCAGGGAGAA AATCCATTAC 
ATTCGGACTG AGGGTAATCA TGGGCTTGAA AAGTTGTCCT GTGATGCAGA TCTAGTCATT 
TTGCTGAGTC TCTTTGAGGA AGAGATTATG TCCTATGTCC CCCTGCAGGC CGCCTTCCAC 
CCTGGGTACA GCTTCTCTCC CCGCTGCTCA CCCTGCTCCT CACCTCAGAA CTCCCCAGGT 
TTACAGAGAG CCAGTGCAAG AGCCCCTTCA CCCTACAGGA GAGACTTTGA GGCCAAGCTC 
CGCAATTTCT ACCGAAAACT GGAAGCCAAA GGATTTGGTC AGGGTCCAGG GAAAATTAAG 
CTCATCATTC GTCGAGACCA CCTGTTGGAG GGAACCTTCA ATCAGGTGAT GGCCTATTCC 
CGGAAGGAGC TCCAGCGAAA CAAGCTCTAC ATCACCTTTG TTGGAGAGGA GGGCCTGGAC 
TACAGTGGTC CTTCGAGAGA ATTCTTCTTC CTTCTGTCTC AGGAGCTCTT TAACCCTTAC 
TACGGACTCT TCGAGTACTC TGCCAATGAC ACCTACACCG TGCAGATCAG CCCCATGTCT 
GCCTTTGTAG AAAACCATCT TGAATGGTTC AGGTTCAGTG GTCGAATCCT AGGCCTGGCT 
CTGATCCATC AGTACCTTCT GGATGCTTTC TTCACAAGAC CCTTCTATAA GGCGCTCCTG 
AGACTACCCT GTGATTTGAG TGACCTGGAA TATCTAGATG AGGAATTCCA CCAGAGCTTG 
CAATGGATGA AGGACAACAA TATCACAGAC ATCCTCGACC TCACGTTCAC GGTTAATGAA 
GAGGTTTTTG GCCAGGTAAC GGAGAGGGAG TTAAAATCTG GAGGTGCCAA CACACAGGTG 
ACAGAGAAGA ACAAGAAGGA GTACATCGAG AGGATGGTGA GGTGGCGGGT AGAGCGCGGC 
GTGGTGCAGC AGACCGAGGC ACTGGTGCGG GGCTTCTACG AGGTGGTGGA CTCAAGGCTG 
GTCTCCGTGT TTGATGCCAG GGAGCTGGAG CTGGTGATCG CTGGCACAGC AGAGATTGAC 
CTGGCCGACT GGCGGAACAA CACTGAGTAC CGGGGAGGTT ATCATGATGG GCATCTTGTG 
ATCCGCTGGT TCTGGGCTGC GGTGGAGCGT TTCAACAATG AGCAGAGACT CAGATTACTG 
CAGTTCGTCA CAGGAACATC AAGCGTGCCC TACGAAGGCT TTGCAGCCCT GCGAGGAAGC 
AACGGGCTTC GGCGTTTCTG CATAGAGAAA TGGGGGAAAA TTACATCTCT TCCCAGGGCA 
CACACGTGCT TCAATCGATT GGATCTTCCA CCTTATCCCT CGTACTCCAT GTTGTATGAA 
AAGCTGTTAA CGGCAGTAGA AGAAACCAGC ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HECW1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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