NHSL2 cDNA ORF clone, Bos taurus(cattle)

The following NHSL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHSL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238679 XM_024988572.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa238680 XM_024988573.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa89357 XM_015470423.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa89357 XM_015461645.2
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa89358 XM_015470424.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa89358 XM_015461646.2
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa238677 XM_024988570.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa238678 XM_024988571.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa89354 XM_015461642.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89354 XM_015470420.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89355 XM_015461643.2
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89355 XM_015470421.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89356 XM_015470422.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89356 XM_015461644.1
Latest version!
Bos taurus NHS like 2 (NHSL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OBa89358 XM_015461646.1 Bos taurus NHS like 2 (NHSL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa89355 XM_015461643.1 Bos taurus NHS like 2 (NHSL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89357 XM_015461645.1 Bos taurus NHS like 2 (NHSL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238679
    Clone ID Related Accession (Same CDS sequence) XM_024988572.1
    Accession Version XM_024988572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTGGCCC AGCCTAGAGC ATGGAATGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA 
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA
    GGCAGTGTGG CCCGCTCTAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT
    GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA
    CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC
    CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA
    GAATCGACAT TCTCTGCTTC CAGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC
    ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAGTC CTTCCTCTGG GAATTCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT
    CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TGCTCAACGA
    AGCCAAGTAT CTGAACGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG
    GAGACAGGCC CAGGTGGGGC CAGCAAGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT
    ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA
    CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT
    GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC
    AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC
    TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC
    TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA
    ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACTGGCAC CACAGTCATT
    GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC
    ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG
    CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG
    CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAGAAAATG
    TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT
    GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGATACAAAT
    TTGGGGGCCA AGCTAGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC CATGGTTACT
    CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC
    ACTGAGAGCC CTGACTACGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTCC AAGCTTAGTC
    CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCACTAACTT CGCCTACATT GGCTATGACT
    CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG
    AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA
    TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT
    AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA
    AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA TGGCTCAAGC GGAGGCCTAT
    GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA TGCCAAGTGT
    CTCCCAACTG ATGACCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC
    AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA
    AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG
    GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA
    GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTTCAGG GTCAAGCGGC
    AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA
    AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA
    CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844340.1
    CDS116..3061
    Translation

    Target ORF information:

    RefSeq Version XM_024988572.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTGGCCC AGCCTAGAGC ATGGAATGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA 
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA
    GGCAGTGTGG CCCGCTCTAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT
    GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA
    CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC
    CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA
    GAATCGACAT TCTCTGCTTC CAGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC
    ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAGTC CTTCCTCTGG GAATTCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT
    CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TGCTCAACGA
    AGCCAAGTAT CTGAACGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG
    GAGACAGGCC CAGGTGGGGC CAGCAAGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT
    ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA
    CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT
    GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC
    AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC
    TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC
    TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA
    ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACTGGCAC CACAGTCATT
    GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC
    ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG
    CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG
    CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAGAAAATG
    TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT
    GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGATACAAAT
    TTGGGGGCCA AGCTAGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC CATGGTTACT
    CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC
    ACTGAGAGCC CTGACTACGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTCC AAGCTTAGTC
    CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCACTAACTT CGCCTACATT GGCTATGACT
    CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG
    AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA
    TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT
    AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA
    AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA TGGCTCAAGC GGAGGCCTAT
    GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA TGCCAAGTGT
    CTCCCAACTG ATGACCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC
    AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA
    AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG
    GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA
    GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTTCAGG GTCAAGCGGC
    AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA
    AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA
    CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238680
    Clone ID Related Accession (Same CDS sequence) XM_024988573.1
    Accession Version XM_024988573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG 
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844341.1
    CDS19..2808
    Translation

    Target ORF information:

    RefSeq Version XM_024988573.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG 
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89357
    Clone ID Related Accession (Same CDS sequence) XM_015470423.1 , XM_015461645.2 , XM_015461645.1
    Accession Version XM_015470423.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325909.1
    CDS862..3831
    Translation

    Target ORF information:

    RefSeq Version XM_015470423.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89357
    Clone ID Related Accession (Same CDS sequence) XM_015470423.1 , XM_015461645.2 , XM_015461645.1
    Accession Version XM_015461645.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317131.1
    CDS75..3044
    Translation

    Target ORF information:

    RefSeq Version XM_015461645.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461645.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89357
    Clone ID Related Accession (Same CDS sequence) XM_015470423.1 , XM_015461645.2 , XM_015461645.1
    Accession Version XM_015461645.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317131.1
    CDS862..3831
    Translation

    Target ORF information:

    RefSeq Version XM_015461645.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89358
    Clone ID Related Accession (Same CDS sequence) XM_015461646.2 , XM_015461646.1 , XM_015470424.1
    Accession Version XM_015461646.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGCCC AGCCTAGAGC ATGGAATGGT CACAGCAACA GCCCAGCAGG CAGTGTGGCC 
    CGCTCTACCA CCTCAGACAT CAGGCCCAGC CATTCAGTTC CAGAAAGTGT TCATGGAAGA
    GTTGCACTTG GTCAGGAAGC TCGGTTCCCA AATCTCACCT CACCAGTACT AAGAAATCCT
    TCTAGTCATC CGGAAGAACC TCCCCAGGCA TGGGGTGCCA CAAAACCCCC TGGAATGGAG
    AGCATGGGGA TGGTGTACAG CATTCCTGGT TCTTGCAATG GACCAACAGA ATCGACATTC
    TCTGCTTCCA GGAAGGGAGA TGCTTTTACC TATGTGACTC CAAGTGCCAC CGCTCAGAGC
    AGTCAAGTCA ATGAAAATGG AAAAAGTCCT TCCTCTGGGA ATTCTTGGGT CTCTCTGCAC
    ACACTGCCAC CTCTTGTTCC TAAGGAGGCT GCTACCCTCT TTGTCACTCG TGATATCCCA
    GCAGGGTGCA GTGGGCCTGC TGGCTACTCT CAGCATCCTG CTCAACGAAG CCAAGTATCT
    GAACGACCCT CCAAGATTGG CCTTCTGACC GGTGGTACCT CAAGGCTGGA GACAGGCCCA
    GGTGGGGCCA GCAAGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGATAC AGGTACCATG
    GATGTGGACT ATGAGGAGGA GCAGAAGGCC AGTGAAGCCT GTGCCCTACC TTATGCCAGT
    ACGAGTTCTG AGGGCAGTAA CAGTGCTGAC AACATTGCCT CCCTCAGTGC CCAACAGGAG
    GCCCATCACA GAAGGCAGAG GTCCAAGAGC ATCTCACTCA AGAAGGCCAA GAAGAAGCCT
    TCCCCTCCGA CTCGCAGTGT CTCACTGGTC AAAGATGAAC CAGTCCTCTT GCCGGAAGGT
    GGGTCAGTAC TACCCAAGGA CCAGAGGCCC AGGAGCCTTT GTCTCTCCTT GGAACACCAA
    GGACATCACT CATCCCACCC AGATACTCAG GGTCACCCAG CTGTGCCAAC CTTCAAAGAC
    CCAGAAGGTA CACAATTCGC CCATCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACTGGCACCA CAGTCATTGA GTGCACCCAA
    GTTCAGGGCA GCTCAGAGTC TCTTGCCTCC CCTTCCACTT CCAGAGCCAC GACGCCTTCC
    CAGCTTTCCA TCGAGGTGGA GGCCAGGGAG GTATCCTCTC CAGGAAGGCC TACTGGGCTG
    ATGTCACCCT CCAGTGGATA CTCCAGCCAG TCAGAGACGC CAACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT GCCCCCTCCA AGCAGCAGTG TCCGGGTGCG TCCAGTGGTA
    CCTGAGAGGA AGTCATCACT ACCCCCAACA TCACCAATGG AGAAAATGTC CAAGTCACGG
    CTATCGTTTG ACCTACCATT GACCTCTTCA ACCAACCTGG ATCTGTCTGG GATGAGTATC
    TCAATCCGAA GCAAAAACAA GGTGAGCCAG CATCACTCAG ATACAAATTT GGGGGCCAAG
    CTAGCCCAGA AAACTAGCCC CAACCAGCCA ATCATGCCCA TGGTTACTCA GTCTGACTTA
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCTGG AAGATGACAC TGAGAGCCCT
    GACTACGCTG AGGAATCAGG AGCTGAAGTC TTCACCTTGC CAGAGAGGAA GATGAAACCT
    CCCATAGCCG AGAAGCCCCC TCTGGCCCGA AGGCCTCCAA GCTTAGTCCA CAAGCCACCA
    TCTGTCCCCG AGGAGTACCC ACTAACTTCG CCTACATTGG CTATGACTCC CAAGAGCTCA
    ATTCAGCACA TAAGGCCACT CCCTCAAGAT ATCTACACAG TGGTGCGGAA ATCAAAGTCC
    TCCAGCTTCC CTGAGAGCAG AAGCCCAGGG GAGTCCACAG CACCCTCATC TCTTGTTTTC
    ACGCCTTTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGGA
    AGTATGGAGG ATGAGGGCCC TAAGGGGAGA GCCCTGCCTG AAAGAATTAG CCTCCAGAGC
    CAAGAAGAAG CTGAGAAAAA GAAAGGCAAG ATTCCACCTC CTGTACCAAA AAAGCCCAGC
    GTGCTGTACC TGCCTCTCAC TTCACCCATG GCTCAAGCGG AGGCCTATGT GACTGAACCA
    AGACTGCCCC TCAGCCCCAT CATCACCCTG GAGGAAGATG CCAAGTGTCT CCCAACTGAT
    GACCACCTGC CATCTGCTGG TACAAGGATG ACCTCAAGGC CACAGTCCAA CAGCGAAAGG
    GAAGCAAGTC CTCCAGGGAG TTTGATGGAA CCAAGCACCG AAGAAAAAAG TGTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAAGAT GATGATGATG TGTTTGTGGC TTCACGTACA
    ACTGAAGATT TATTTACTGT GATCCACAGG TCCAAAAGAA AGCTGCTTGG CTGGAAGGAA
    CCTGGTGAGA CCTTTGCTGG CAGCAGCAGA CCAAGCTCCC ACTCACCAGT AAGGAACCCA
    CCTGAGTCTC CGGTCAGTGA GTTGGCTGCC TCTTCAGGGT CAAGCGGCAG TGCCAACCTA
    GATGCTGGCA GAAATGATGA TTTCAAGGCC TTGCTACAGA AGAAGGGAAG CAAGGCAACT
    CCAAGGTCCC GCCCCTCAGC AGCCGAACTG CTGAAGACCA CTAACCCACT GGCTCGGCGA
    ATTATTGCAC AATTTTCAAA AGACTACAAA ACCCCTGATA ACCCCAGTAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317132.1
    CDS123..2936
    Translation

    Target ORF information:

    RefSeq Version XM_015461646.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461646.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGCCC AGCCTAGAGC ATGGAATGGT CACAGCAACA GCCCAGCAGG CAGTGTGGCC 
    CGCTCTACCA CCTCAGACAT CAGGCCCAGC CATTCAGTTC CAGAAAGTGT TCATGGAAGA
    GTTGCACTTG GTCAGGAAGC TCGGTTCCCA AATCTCACCT CACCAGTACT AAGAAATCCT
    TCTAGTCATC CGGAAGAACC TCCCCAGGCA TGGGGTGCCA CAAAACCCCC TGGAATGGAG
    AGCATGGGGA TGGTGTACAG CATTCCTGGT TCTTGCAATG GACCAACAGA ATCGACATTC
    TCTGCTTCCA GGAAGGGAGA TGCTTTTACC TATGTGACTC CAAGTGCCAC CGCTCAGAGC
    AGTCAAGTCA ATGAAAATGG AAAAAGTCCT TCCTCTGGGA ATTCTTGGGT CTCTCTGCAC
    ACACTGCCAC CTCTTGTTCC TAAGGAGGCT GCTACCCTCT TTGTCACTCG TGATATCCCA
    GCAGGGTGCA GTGGGCCTGC TGGCTACTCT CAGCATCCTG CTCAACGAAG CCAAGTATCT
    GAACGACCCT CCAAGATTGG CCTTCTGACC GGTGGTACCT CAAGGCTGGA GACAGGCCCA
    GGTGGGGCCA GCAAGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGATAC AGGTACCATG
    GATGTGGACT ATGAGGAGGA GCAGAAGGCC AGTGAAGCCT GTGCCCTACC TTATGCCAGT
    ACGAGTTCTG AGGGCAGTAA CAGTGCTGAC AACATTGCCT CCCTCAGTGC CCAACAGGAG
    GCCCATCACA GAAGGCAGAG GTCCAAGAGC ATCTCACTCA AGAAGGCCAA GAAGAAGCCT
    TCCCCTCCGA CTCGCAGTGT CTCACTGGTC AAAGATGAAC CAGTCCTCTT GCCGGAAGGT
    GGGTCAGTAC TACCCAAGGA CCAGAGGCCC AGGAGCCTTT GTCTCTCCTT GGAACACCAA
    GGACATCACT CATCCCACCC AGATACTCAG GGTCACCCAG CTGTGCCAAC CTTCAAAGAC
    CCAGAAGGTA CACAATTCGC CCATCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACTGGCACCA CAGTCATTGA GTGCACCCAA
    GTTCAGGGCA GCTCAGAGTC TCTTGCCTCC CCTTCCACTT CCAGAGCCAC GACGCCTTCC
    CAGCTTTCCA TCGAGGTGGA GGCCAGGGAG GTATCCTCTC CAGGAAGGCC TACTGGGCTG
    ATGTCACCCT CCAGTGGATA CTCCAGCCAG TCAGAGACGC CAACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT GCCCCCTCCA AGCAGCAGTG TCCGGGTGCG TCCAGTGGTA
    CCTGAGAGGA AGTCATCACT ACCCCCAACA TCACCAATGG AGAAAATGTC CAAGTCACGG
    CTATCGTTTG ACCTACCATT GACCTCTTCA ACCAACCTGG ATCTGTCTGG GATGAGTATC
    TCAATCCGAA GCAAAAACAA GGTGAGCCAG CATCACTCAG ATACAAATTT GGGGGCCAAG
    CTAGCCCAGA AAACTAGCCC CAACCAGCCA ATCATGCCCA TGGTTACTCA GTCTGACTTA
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCTGG AAGATGACAC TGAGAGCCCT
    GACTACGCTG AGGAATCAGG AGCTGAAGTC TTCACCTTGC CAGAGAGGAA GATGAAACCT
    CCCATAGCCG AGAAGCCCCC TCTGGCCCGA AGGCCTCCAA GCTTAGTCCA CAAGCCACCA
    TCTGTCCCCG AGGAGTACCC ACTAACTTCG CCTACATTGG CTATGACTCC CAAGAGCTCA
    ATTCAGCACA TAAGGCCACT CCCTCAAGAT ATCTACACAG TGGTGCGGAA ATCAAAGTCC
    TCCAGCTTCC CTGAGAGCAG AAGCCCAGGG GAGTCCACAG CACCCTCATC TCTTGTTTTC
    ACGCCTTTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGGA
    AGTATGGAGG ATGAGGGCCC TAAGGGGAGA GCCCTGCCTG AAAGAATTAG CCTCCAGAGC
    CAAGAAGAAG CTGAGAAAAA GAAAGGCAAG ATTCCACCTC CTGTACCAAA AAAGCCCAGC
    GTGCTGTACC TGCCTCTCAC TTCACCCATG GCTCAAGCGG AGGCCTATGT GACTGAACCA
    AGACTGCCCC TCAGCCCCAT CATCACCCTG GAGGAAGATG CCAAGTGTCT CCCAACTGAT
    GACCACCTGC CATCTGCTGG TACAAGGATG ACCTCAAGGC CACAGTCCAA CAGCGAAAGG
    GAAGCAAGTC CTCCAGGGAG TTTGATGGAA CCAAGCACCG AAGAAAAAAG TGTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAAGAT GATGATGATG TGTTTGTGGC TTCACGTACA
    ACTGAAGATT TATTTACTGT GATCCACAGG TCCAAAAGAA AGCTGCTTGG CTGGAAGGAA
    CCTGGTGAGA CCTTTGCTGG CAGCAGCAGA CCAAGCTCCC ACTCACCAGT AAGGAACCCA
    CCTGAGTCTC CGGTCAGTGA GTTGGCTGCC TCTTCAGGGT CAAGCGGCAG TGCCAACCTA
    GATGCTGGCA GAAATGATGA TTTCAAGGCC TTGCTACAGA AGAAGGGAAG CAAGGCAACT
    CCAAGGTCCC GCCCCTCAGC AGCCGAACTG CTGAAGACCA CTAACCCACT GGCTCGGCGA
    ATTATTGCAC AATTTTCAAA AGACTACAAA ACCCCTGATA ACCCCAGTAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89358
    Clone ID Related Accession (Same CDS sequence) XM_015461646.2 , XM_015461646.1 , XM_015470424.1
    Accession Version XM_015461646.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGCCC AGCCTAGAGC ATGGAATGGT CACAGCAACA GCCCAGCAGG CAGTGTGGCC 
    CGCTCTACCA CCTCAGACAT CAGGCCCAGC CATTCAGTTC CAGAAAGTGT TCATGGAAGA
    GTTGCACTTG GTCAGGAAGC TCGGTTCCCA AATCTCACCT CACCAGTACT AAGAAATCCT
    TCTAGTCATC CGGAAGAACC TCCCCAGGCA TGGGGTGCCA CAAAACCCCC TGGAATGGAG
    AGCATGGGGA TGGTGTACAG CATTCCTGGT TCTTGCAATG GACCAACAGA ATCGACATTC
    TCTGCTTCCA GGAAGGGAGA TGCTTTTACC TATGTGACTC CAAGTGCCAC CGCTCAGAGC
    AGTCAAGTCA ATGAAAATGG AAAAAGTCCT TCCTCTGGGA ATTCTTGGGT CTCTCTGCAC
    ACACTGCCAC CTCTTGTTCC TAAGGAGGCT GCTACCCTCT TTGTCACTCG TGATATCCCA
    GCAGGGTGCA GTGGGCCTGC TGGCTACTCT CAGCATCCTG CTCAACGAAG CCAAGTATCT
    GAACGACCCT CCAAGATTGG CCTTCTGACC GGTGGTACCT CAAGGCTGGA GACAGGCCCA
    GGTGGGGCCA GCAAGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGATAC AGGTACCATG
    GATGTGGACT ATGAGGAGGA GCAGAAGGCC AGTGAAGCCT GTGCCCTACC TTATGCCAGT
    ACGAGTTCTG AGGGCAGTAA CAGTGCTGAC AACATTGCCT CCCTCAGTGC CCAACAGGAG
    GCCCATCACA GAAGGCAGAG GTCCAAGAGC ATCTCACTCA AGAAGGCCAA GAAGAAGCCT
    TCCCCTCCGA CTCGCAGTGT CTCACTGGTC AAAGATGAAC CAGTCCTCTT GCCGGAAGGT
    GGGTCAGTAC TACCCAAGGA CCAGAGGCCC AGGAGCCTTT GTCTCTCCTT GGAACACCAA
    GGACATCACT CATCCCACCC AGATACTCAG GGTCACCCAG CTGTGCCAAC CTTCAAAGAC
    CCAGAAGGTA CACAATTCGC CCATCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACTGGCACCA CAGTCATTGA GTGCACCCAA
    GTTCAGGGCA GCTCAGAGTC TCTTGCCTCC CCTTCCACTT CCAGAGCCAC GACGCCTTCC
    CAGCTTTCCA TCGAGGTGGA GGCCAGGGAG GTATCCTCTC CAGGAAGGCC TACTGGGCTG
    ATGTCACCCT CCAGTGGATA CTCCAGCCAG TCAGAGACGC CAACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT GCCCCCTCCA AGCAGCAGTG TCCGGGTGCG TCCAGTGGTA
    CCTGAGAGGA AGTCATCACT ACCCCCAACA TCACCAATGG AGAAAATGTC CAAGTCACGG
    CTATCGTTTG ACCTACCATT GACCTCTTCA ACCAACCTGG ATCTGTCTGG GATGAGTATC
    TCAATCCGAA GCAAAAACAA GGTGAGCCAG CATCACTCAG ATACAAATTT GGGGGCCAAG
    CTAGCCCAGA AAACTAGCCC CAACCAGCCA ATCATGCCCA TGGTTACTCA GTCTGACTTA
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCTGG AAGATGACAC TGAGAGCCCT
    GACTACGCTG AGGAATCAGG AGCTGAAGTC TTCACCTTGC CAGAGAGGAA GATGAAACCT
    CCCATAGCCG AGAAGCCCCC TCTGGCCCGA AGGCCTCCAA GCTTAGTCCA CAAGCCACCA
    TCTGTCCCCG AGGAGTACCC ACTAACTTCG CCTACATTGG CTATGACTCC CAAGAGCTCA
    ATTCAGCACA TAAGGCCACT CCCTCAAGAT ATCTACACAG TGGTGCGGAA ATCAAAGTCC
    TCCAGCTTCC CTGAGAGCAG AAGCCCAGGG GAGTCCACAG CACCCTCATC TCTTGTTTTC
    ACGCCTTTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGGA
    AGTATGGAGG ATGAGGGCCC TAAGGGGAGA GCCCTGCCTG AAAGAATTAG CCTCCAGAGC
    CAAGAAGAAG CTGAGAAAAA GAAAGGCAAG ATTCCACCTC CTGTACCAAA AAAGCCCAGC
    GTGCTGTACC TGCCTCTCAC TTCACCCATG GCTCAAGCGG AGGCCTATGT GACTGAACCA
    AGACTGCCCC TCAGCCCCAT CATCACCCTG GAGGAAGATG CCAAGTGTCT CCCAACTGAT
    GACCACCTGC CATCTGCTGG TACAAGGATG ACCTCAAGGC CACAGTCCAA CAGCGAAAGG
    GAAGCAAGTC CTCCAGGGAG TTTGATGGAA CCAAGCACCG AAGAAAAAAG TGTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAAGAT GATGATGATG TGTTTGTGGC TTCACGTACA
    ACTGAAGATT TATTTACTGT GATCCACAGG TCCAAAAGAA AGCTGCTTGG CTGGAAGGAA
    CCTGGTGAGA CCTTTGCTGG CAGCAGCAGA CCAAGCTCCC ACTCACCAGT AAGGAACCCA
    CCTGAGTCTC CGGTCAGTGA GTTGGCTGCC TCTTCAGGGT CAAGCGGCAG TGCCAACCTA
    GATGCTGGCA GAAATGATGA TTTCAAGGCC TTGCTACAGA AGAAGGGAAG CAAGGCAACT
    CCAAGGTCCC GCCCCTCAGC AGCCGAACTG CTGAAGACCA CTAACCCACT GGCTCGGCGA
    ATTATTGCAC AATTTTCAAA AGACTACAAA ACCCCTGATA ACCCCAGTAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317132.1
    CDS860..3673
    Translation

    Target ORF information:

    RefSeq Version XM_015461646.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGCCC AGCCTAGAGC ATGGAATGGT CACAGCAACA GCCCAGCAGG CAGTGTGGCC 
    CGCTCTACCA CCTCAGACAT CAGGCCCAGC CATTCAGTTC CAGAAAGTGT TCATGGAAGA
    GTTGCACTTG GTCAGGAAGC TCGGTTCCCA AATCTCACCT CACCAGTACT AAGAAATCCT
    TCTAGTCATC CGGAAGAACC TCCCCAGGCA TGGGGTGCCA CAAAACCCCC TGGAATGGAG
    AGCATGGGGA TGGTGTACAG CATTCCTGGT TCTTGCAATG GACCAACAGA ATCGACATTC
    TCTGCTTCCA GGAAGGGAGA TGCTTTTACC TATGTGACTC CAAGTGCCAC CGCTCAGAGC
    AGTCAAGTCA ATGAAAATGG AAAAAGTCCT TCCTCTGGGA ATTCTTGGGT CTCTCTGCAC
    ACACTGCCAC CTCTTGTTCC TAAGGAGGCT GCTACCCTCT TTGTCACTCG TGATATCCCA
    GCAGGGTGCA GTGGGCCTGC TGGCTACTCT CAGCATCCTG CTCAACGAAG CCAAGTATCT
    GAACGACCCT CCAAGATTGG CCTTCTGACC GGTGGTACCT CAAGGCTGGA GACAGGCCCA
    GGTGGGGCCA GCAAGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGATAC AGGTACCATG
    GATGTGGACT ATGAGGAGGA GCAGAAGGCC AGTGAAGCCT GTGCCCTACC TTATGCCAGT
    ACGAGTTCTG AGGGCAGTAA CAGTGCTGAC AACATTGCCT CCCTCAGTGC CCAACAGGAG
    GCCCATCACA GAAGGCAGAG GTCCAAGAGC ATCTCACTCA AGAAGGCCAA GAAGAAGCCT
    TCCCCTCCGA CTCGCAGTGT CTCACTGGTC AAAGATGAAC CAGTCCTCTT GCCGGAAGGT
    GGGTCAGTAC TACCCAAGGA CCAGAGGCCC AGGAGCCTTT GTCTCTCCTT GGAACACCAA
    GGACATCACT CATCCCACCC AGATACTCAG GGTCACCCAG CTGTGCCAAC CTTCAAAGAC
    CCAGAAGGTA CACAATTCGC CCATCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACTGGCACCA CAGTCATTGA GTGCACCCAA
    GTTCAGGGCA GCTCAGAGTC TCTTGCCTCC CCTTCCACTT CCAGAGCCAC GACGCCTTCC
    CAGCTTTCCA TCGAGGTGGA GGCCAGGGAG GTATCCTCTC CAGGAAGGCC TACTGGGCTG
    ATGTCACCCT CCAGTGGATA CTCCAGCCAG TCAGAGACGC CAACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT GCCCCCTCCA AGCAGCAGTG TCCGGGTGCG TCCAGTGGTA
    CCTGAGAGGA AGTCATCACT ACCCCCAACA TCACCAATGG AGAAAATGTC CAAGTCACGG
    CTATCGTTTG ACCTACCATT GACCTCTTCA ACCAACCTGG ATCTGTCTGG GATGAGTATC
    TCAATCCGAA GCAAAAACAA GGTGAGCCAG CATCACTCAG ATACAAATTT GGGGGCCAAG
    CTAGCCCAGA AAACTAGCCC CAACCAGCCA ATCATGCCCA TGGTTACTCA GTCTGACTTA
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCTGG AAGATGACAC TGAGAGCCCT
    GACTACGCTG AGGAATCAGG AGCTGAAGTC TTCACCTTGC CAGAGAGGAA GATGAAACCT
    CCCATAGCCG AGAAGCCCCC TCTGGCCCGA AGGCCTCCAA GCTTAGTCCA CAAGCCACCA
    TCTGTCCCCG AGGAGTACCC ACTAACTTCG CCTACATTGG CTATGACTCC CAAGAGCTCA
    ATTCAGCACA TAAGGCCACT CCCTCAAGAT ATCTACACAG TGGTGCGGAA ATCAAAGTCC
    TCCAGCTTCC CTGAGAGCAG AAGCCCAGGG GAGTCCACAG CACCCTCATC TCTTGTTTTC
    ACGCCTTTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGGA
    AGTATGGAGG ATGAGGGCCC TAAGGGGAGA GCCCTGCCTG AAAGAATTAG CCTCCAGAGC
    CAAGAAGAAG CTGAGAAAAA GAAAGGCAAG ATTCCACCTC CTGTACCAAA AAAGCCCAGC
    GTGCTGTACC TGCCTCTCAC TTCACCCATG GCTCAAGCGG AGGCCTATGT GACTGAACCA
    AGACTGCCCC TCAGCCCCAT CATCACCCTG GAGGAAGATG CCAAGTGTCT CCCAACTGAT
    GACCACCTGC CATCTGCTGG TACAAGGATG ACCTCAAGGC CACAGTCCAA CAGCGAAAGG
    GAAGCAAGTC CTCCAGGGAG TTTGATGGAA CCAAGCACCG AAGAAAAAAG TGTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAAGAT GATGATGATG TGTTTGTGGC TTCACGTACA
    ACTGAAGATT TATTTACTGT GATCCACAGG TCCAAAAGAA AGCTGCTTGG CTGGAAGGAA
    CCTGGTGAGA CCTTTGCTGG CAGCAGCAGA CCAAGCTCCC ACTCACCAGT AAGGAACCCA
    CCTGAGTCTC CGGTCAGTGA GTTGGCTGCC TCTTCAGGGT CAAGCGGCAG TGCCAACCTA
    GATGCTGGCA GAAATGATGA TTTCAAGGCC TTGCTACAGA AGAAGGGAAG CAAGGCAACT
    CCAAGGTCCC GCCCCTCAGC AGCCGAACTG CTGAAGACCA CTAACCCACT GGCTCGGCGA
    ATTATTGCAC AATTTTCAAA AGACTACAAA ACCCCTGATA ACCCCAGTAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89358
    Clone ID Related Accession (Same CDS sequence) XM_015461646.2 , XM_015461646.1 , XM_015470424.1
    Accession Version XM_015470424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGCCC AGCCTAGAGC ATGGAATGGT CACAGCAACA GCCCAGCAGG CAGTGTGGCC 
    CGCTCTACCA CCTCAGACAT CAGGCCCAGC CATTCAGTTC CAGAAAGTGT TCATGGAAGA
    GTTGCACTTG GTCAGGAAGC TCGGTTCCCA AATCTCACCT CACCAGTACT AAGAAATCCT
    TCTAGTCATC CGGAAGAACC TCCCCAGGCA TGGGGTGCCA CAAAACCCCC TGGAATGGAG
    AGCATGGGGA TGGTGTACAG CATTCCTGGT TCTTGCAATG GACCAACAGA ATCGACATTC
    TCTGCTTCCA GGAAGGGAGA TGCTTTTACC TATGTGACTC CAAGTGCCAC CGCTCAGAGC
    AGTCAAGTCA ATGAAAATGG AAAAAGTCCT TCCTCTGGGA ATTCTTGGGT CTCTCTGCAC
    ACACTGCCAC CTCTTGTTCC TAAGGAGGCT GCTACCCTCT TTGTCACTCG TGATATCCCA
    GCAGGGTGCA GTGGGCCTGC TGGCTACTCT CAGCATCCTG CTCAACGAAG CCAAGTATCT
    GAACGACCCT CCAAGATTGG CCTTCTGACC GGTGGTACCT CAAGGCTGGA GACAGGCCCA
    GGTGGGGCCA GCAAGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGATAC AGGTACCATG
    GATGTGGACT ATGAGGAGGA GCAGAAGGCC AGTGAAGCCT GTGCCCTACC TTATGCCAGT
    ACGAGTTCTG AGGGCAGTAA CAGTGCTGAC AACATTGCCT CCCTCAGTGC CCAACAGGAG
    GCCCATCACA GAAGGCAGAG GTCCAAGAGC ATCTCACTCA AGAAGGCCAA GAAGAAGCCT
    TCCCCTCCGA CTCGCAGTGT CTCACTGGTC AAAGATGAAC CAGTCCTCTT GCCGGAAGGT
    GGGTCAGTAC TACCCAAGGA CCAGAGGCCC AGGAGCCTTT GTCTCTCCTT GGAACACCAA
    GGACATCACT CATCCCACCC AGATACTCAG GGTCACCCAG CTGTGCCAAC CTTCAAAGAC
    CCAGAAGGTA CACAATTCGC CCATCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACTGGCACCA CAGTCATTGA GTGCACCCAA
    GTTCAGGGCA GCTCAGAGTC TCTTGCCTCC CCTTCCACTT CCAGAGCCAC GACGCCTTCC
    CAGCTTTCCA TCGAGGTGGA GGCCAGGGAG GTATCCTCTC CAGGAAGGCC TACTGGGCTG
    ATGTCACCCT CCAGTGGATA CTCCAGCCAG TCAGAGACGC CAACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT GCCCCCTCCA AGCAGCAGTG TCCGGGTGCG TCCAGTGGTA
    CCTGAGAGGA AGTCATCACT ACCCCCAACA TCACCAATGG AGAAAATGTC CAAGTCACGG
    CTATCGTTTG ACCTACCATT GACCTCTTCA ACCAACCTGG ATCTGTCTGG GATGAGTATC
    TCAATCCGAA GCAAAAACAA GGTGAGCCAG CATCACTCAG ATACAAATTT GGGGGCCAAG
    CTAGCCCAGA AAACTAGCCC CAACCAGCCA ATCATGCCCA TGGTTACTCA GTCTGACTTA
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCTGG AAGATGACAC TGAGAGCCCT
    GACTACGCTG AGGAATCAGG AGCTGAAGTC TTCACCTTGC CAGAGAGGAA GATGAAACCT
    CCCATAGCCG AGAAGCCCCC TCTGGCCCGA AGGCCTCCAA GCTTAGTCCA CAAGCCACCA
    TCTGTCCCCG AGGAGTACCC ACTAACTTCG CCTACATTGG CTATGACTCC CAAGAGCTCA
    ATTCAGCACA TAAGGCCACT CCCTCAAGAT ATCTACACAG TGGTGCGGAA ATCAAAGTCC
    TCCAGCTTCC CTGAGAGCAG AAGCCCAGGG GAGTCCACAG CACCCTCATC TCTTGTTTTC
    ACGCCTTTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGGA
    AGTATGGAGG ATGAGGGCCC TAAGGGGAGA GCCCTGCCTG AAAGAATTAG CCTCCAGAGC
    CAAGAAGAAG CTGAGAAAAA GAAAGGCAAG ATTCCACCTC CTGTACCAAA AAAGCCCAGC
    GTGCTGTACC TGCCTCTCAC TTCACCCATG GCTCAAGCGG AGGCCTATGT GACTGAACCA
    AGACTGCCCC TCAGCCCCAT CATCACCCTG GAGGAAGATG CCAAGTGTCT CCCAACTGAT
    GACCACCTGC CATCTGCTGG TACAAGGATG ACCTCAAGGC CACAGTCCAA CAGCGAAAGG
    GAAGCAAGTC CTCCAGGGAG TTTGATGGAA CCAAGCACCG AAGAAAAAAG TGTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAAGAT GATGATGATG TGTTTGTGGC TTCACGTACA
    ACTGAAGATT TATTTACTGT GATCCACAGG TCCAAAAGAA AGCTGCTTGG CTGGAAGGAA
    CCTGGTGAGA CCTTTGCTGG CAGCAGCAGA CCAAGCTCCC ACTCACCAGT AAGGAACCCA
    CCTGAGTCTC CGGTCAGTGA GTTGGCTGCC TCTTCAGGGT CAAGCGGCAG TGCCAACCTA
    GATGCTGGCA GAAATGATGA TTTCAAGGCC TTGCTACAGA AGAAGGGAAG CAAGGCAACT
    CCAAGGTCCC GCCCCTCAGC AGCCGAACTG CTGAAGACCA CTAACCCACT GGCTCGGCGA
    ATTATTGCAC AATTTTCAAA AGACTACAAA ACCCCTGATA ACCCCAGTAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325910.1
    CDS860..3673
    Translation

    Target ORF information:

    RefSeq Version XM_015470424.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGCCC AGCCTAGAGC ATGGAATGGT CACAGCAACA GCCCAGCAGG CAGTGTGGCC 
    CGCTCTACCA CCTCAGACAT CAGGCCCAGC CATTCAGTTC CAGAAAGTGT TCATGGAAGA
    GTTGCACTTG GTCAGGAAGC TCGGTTCCCA AATCTCACCT CACCAGTACT AAGAAATCCT
    TCTAGTCATC CGGAAGAACC TCCCCAGGCA TGGGGTGCCA CAAAACCCCC TGGAATGGAG
    AGCATGGGGA TGGTGTACAG CATTCCTGGT TCTTGCAATG GACCAACAGA ATCGACATTC
    TCTGCTTCCA GGAAGGGAGA TGCTTTTACC TATGTGACTC CAAGTGCCAC CGCTCAGAGC
    AGTCAAGTCA ATGAAAATGG AAAAAGTCCT TCCTCTGGGA ATTCTTGGGT CTCTCTGCAC
    ACACTGCCAC CTCTTGTTCC TAAGGAGGCT GCTACCCTCT TTGTCACTCG TGATATCCCA
    GCAGGGTGCA GTGGGCCTGC TGGCTACTCT CAGCATCCTG CTCAACGAAG CCAAGTATCT
    GAACGACCCT CCAAGATTGG CCTTCTGACC GGTGGTACCT CAAGGCTGGA GACAGGCCCA
    GGTGGGGCCA GCAAGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGATAC AGGTACCATG
    GATGTGGACT ATGAGGAGGA GCAGAAGGCC AGTGAAGCCT GTGCCCTACC TTATGCCAGT
    ACGAGTTCTG AGGGCAGTAA CAGTGCTGAC AACATTGCCT CCCTCAGTGC CCAACAGGAG
    GCCCATCACA GAAGGCAGAG GTCCAAGAGC ATCTCACTCA AGAAGGCCAA GAAGAAGCCT
    TCCCCTCCGA CTCGCAGTGT CTCACTGGTC AAAGATGAAC CAGTCCTCTT GCCGGAAGGT
    GGGTCAGTAC TACCCAAGGA CCAGAGGCCC AGGAGCCTTT GTCTCTCCTT GGAACACCAA
    GGACATCACT CATCCCACCC AGATACTCAG GGTCACCCAG CTGTGCCAAC CTTCAAAGAC
    CCAGAAGGTA CACAATTCGC CCATCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACTGGCACCA CAGTCATTGA GTGCACCCAA
    GTTCAGGGCA GCTCAGAGTC TCTTGCCTCC CCTTCCACTT CCAGAGCCAC GACGCCTTCC
    CAGCTTTCCA TCGAGGTGGA GGCCAGGGAG GTATCCTCTC CAGGAAGGCC TACTGGGCTG
    ATGTCACCCT CCAGTGGATA CTCCAGCCAG TCAGAGACGC CAACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT GCCCCCTCCA AGCAGCAGTG TCCGGGTGCG TCCAGTGGTA
    CCTGAGAGGA AGTCATCACT ACCCCCAACA TCACCAATGG AGAAAATGTC CAAGTCACGG
    CTATCGTTTG ACCTACCATT GACCTCTTCA ACCAACCTGG ATCTGTCTGG GATGAGTATC
    TCAATCCGAA GCAAAAACAA GGTGAGCCAG CATCACTCAG ATACAAATTT GGGGGCCAAG
    CTAGCCCAGA AAACTAGCCC CAACCAGCCA ATCATGCCCA TGGTTACTCA GTCTGACTTA
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCTGG AAGATGACAC TGAGAGCCCT
    GACTACGCTG AGGAATCAGG AGCTGAAGTC TTCACCTTGC CAGAGAGGAA GATGAAACCT
    CCCATAGCCG AGAAGCCCCC TCTGGCCCGA AGGCCTCCAA GCTTAGTCCA CAAGCCACCA
    TCTGTCCCCG AGGAGTACCC ACTAACTTCG CCTACATTGG CTATGACTCC CAAGAGCTCA
    ATTCAGCACA TAAGGCCACT CCCTCAAGAT ATCTACACAG TGGTGCGGAA ATCAAAGTCC
    TCCAGCTTCC CTGAGAGCAG AAGCCCAGGG GAGTCCACAG CACCCTCATC TCTTGTTTTC
    ACGCCTTTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGGA
    AGTATGGAGG ATGAGGGCCC TAAGGGGAGA GCCCTGCCTG AAAGAATTAG CCTCCAGAGC
    CAAGAAGAAG CTGAGAAAAA GAAAGGCAAG ATTCCACCTC CTGTACCAAA AAAGCCCAGC
    GTGCTGTACC TGCCTCTCAC TTCACCCATG GCTCAAGCGG AGGCCTATGT GACTGAACCA
    AGACTGCCCC TCAGCCCCAT CATCACCCTG GAGGAAGATG CCAAGTGTCT CCCAACTGAT
    GACCACCTGC CATCTGCTGG TACAAGGATG ACCTCAAGGC CACAGTCCAA CAGCGAAAGG
    GAAGCAAGTC CTCCAGGGAG TTTGATGGAA CCAAGCACCG AAGAAAAAAG TGTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAAGAT GATGATGATG TGTTTGTGGC TTCACGTACA
    ACTGAAGATT TATTTACTGT GATCCACAGG TCCAAAAGAA AGCTGCTTGG CTGGAAGGAA
    CCTGGTGAGA CCTTTGCTGG CAGCAGCAGA CCAAGCTCCC ACTCACCAGT AAGGAACCCA
    CCTGAGTCTC CGGTCAGTGA GTTGGCTGCC TCTTCAGGGT CAAGCGGCAG TGCCAACCTA
    GATGCTGGCA GAAATGATGA TTTCAAGGCC TTGCTACAGA AGAAGGGAAG CAAGGCAACT
    CCAAGGTCCC GCCCCTCAGC AGCCGAACTG CTGAAGACCA CTAACCCACT GGCTCGGCGA
    ATTATTGCAC AATTTTCAAA AGACTACAAA ACCCCTGATA ACCCCAGTAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238677
    Clone ID Related Accession (Same CDS sequence) XM_024988570.1
    Accession Version XM_024988570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTGGCCC AGCCTAGAGC ATGGAATGAA GAATATGAGG AACAGTACTC GGAGGCCAGA 
    CTTCTGGGGC AGACCTTCCG CTCTGCTGAT GAGGCCCCTG AGCCCACCCC CAGCCCAAGG
    CTCCAGTCTG CCAGGCGTCT GGAGTTTGTA CTGATGCCCA CAAAATGGCA GCTGAGCGAG
    GATGAGACTA CCATGCAGGG TGTGAGGGCC CCTGAGGCCT CCCTGAGCCT GCCTACCACA
    GCCGACAAGC AAGCTGCCTG GAATAGCCCC TTTCCTCTGC CCATCCTAGA GGAGAAGCGG
    TGGCTTCAGT CTTGTCCCAC ACACTCTGAC ATCGTGCCCA TCAACGTCTC CGGGCAGCAG
    TTTGATAAAC ATGCAAGTTT GCGACACTCG TTGTTTAACA CAGAGACAGC CGTGAACCCC
    AAGTCCACCC TGAGGCGGAG GCGGACCATT ATTGGATTCT CTAACTATTC CCAGCGAGAC
    CAAGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844338.1
    CDS16..3285
    Translation

    Target ORF information:

    RefSeq Version XM_024988570.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTGGCCC AGCCTAGAGC ATGGAATGAA GAATATGAGG AACAGTACTC GGAGGCCAGA 
    CTTCTGGGGC AGACCTTCCG CTCTGCTGAT GAGGCCCCTG AGCCCACCCC CAGCCCAAGG
    CTCCAGTCTG CCAGGCGTCT GGAGTTTGTA CTGATGCCCA CAAAATGGCA GCTGAGCGAG
    GATGAGACTA CCATGCAGGG TGTGAGGGCC CCTGAGGCCT CCCTGAGCCT GCCTACCACA
    GCCGACAAGC AAGCTGCCTG GAATAGCCCC TTTCCTCTGC CCATCCTAGA GGAGAAGCGG
    TGGCTTCAGT CTTGTCCCAC ACACTCTGAC ATCGTGCCCA TCAACGTCTC CGGGCAGCAG
    TTTGATAAAC ATGCAAGTTT GCGACACTCG TTGTTTAACA CAGAGACAGC CGTGAACCCC
    AAGTCCACCC TGAGGCGGAG GCGGACCATT ATTGGATTCT CTAACTATTC CCAGCGAGAC
    CAAGGTCACA GCAACAGCCC AGCAGGCAGT GTGGCCCGCT CTACCACCTC AGACATCAGG
    CCCAGCCATT CAGTTCCAGA AAGTGTTCAT GGAAGAGTTG CACTTGGTCA GGAAGCTCGG
    TTCCCAAATC TCACCTCACC AGTACTAAGA AATCCTTCTA GTCATCCGGA AGAACCTCCC
    CAGGCATGGG GTGCCACAAA ACCCCCTGGA ATGGAGAGCA TGGGGATGGT GTACAGCATT
    CCTGGTTCTT GCAATGGACC AACAGAATCG ACATTCTCTG CTTCCAGGAA GGGAGATGCT
    TTTACCTATG TGACTCCAAG TGCCACCGCT CAGAGCAGTC AAGTCAATGA AAATGGAAAA
    AGTCCTTCCT CTGGGAATTC TTGGGTCTCT CTGCACACAC TGCCACCTCT TGTTCCTAAG
    GAGGCTGCTA CCCTCTTTGT CACTCGTGAT ATCCCAGCAG GGTGCAGTGG GCCTGCTGGC
    TACTCTCAGC ATCCTGCTCA ACGAAGCCAA GTATCTGAAC GACCCTCCAA GATTGGCCTT
    CTGACCGGTG GTACCTCAAG GCTGGAGACA GGCCCAGGTG GGGCCAGCAA GTTCCGGGAG
    CGGTCACTGT CTGTACCCAC AGATACAGGT ACCATGGATG TGGACTATGA GGAGGAGCAG
    AAGGCCAGTG AAGCCTGTGC CCTACCTTAT GCCAGTACGA GTTCTGAGGG CAGTAACAGT
    GCTGACAACA TTGCCTCCCT CAGTGCCCAA CAGGAGGCCC ATCACAGAAG GCAGAGGTCC
    AAGAGCATCT CACTCAAGAA GGCCAAGAAG AAGCCTTCCC CTCCGACTCG CAGTGTCTCA
    CTGGTCAAAG ATGAACCAGT CCTCTTGCCG GAAGGTGGGT CAGTACTACC CAAGGACCAG
    AGGCCCAGGA GCCTTTGTCT CTCCTTGGAA CACCAAGGAC ATCACTCATC CCACCCAGAT
    ACTCAGGGTC ACCCAGCTGT GCCAACCTTC AAAGACCCAG AAGGTACACA ATTCGCCCAT
    CACTGGTATC TTACTGACTG GAAGTCTGGT GACACCTACC AGTCCTTGTC CAGCTCCAGC
    ACTGCCACTG GCACCACAGT CATTGAGTGC ACCCAAGTTC AGGGCAGCTC AGAGTCTCTT
    GCCTCCCCTT CCACTTCCAG AGCCACGACG CCTTCCCAGC TTTCCATCGA GGTGGAGGCC
    AGGGAGGTAT CCTCTCCAGG AAGGCCTACT GGGCTGATGT CACCCTCCAG TGGATACTCC
    AGCCAGTCAG AGACGCCAAC ACCCACTGTC TCCATGTCCT TGACCCTGGG CCACTTGCCC
    CCTCCAAGCA GCAGTGTCCG GGTGCGTCCA GTGGTACCTG AGAGGAAGTC ATCACTACCC
    CCAACATCAC CAATGGAGAA AATGTCCAAG TCACGGCTAT CGTTTGACCT ACCATTGACC
    TCTTCAACCA ACCTGGATCT GTCTGGGATG AGTATCTCAA TCCGAAGCAA AAACAAGGTG
    AGCCAGCATC ACTCAGATAC AAATTTGGGG GCCAAGCTAG CCCAGAAAAC TAGCCCCAAC
    CAGCCAATCA TGCCCATGGT TACTCAGTCT GACTTACGTT CTGTTCGCCT GAGGTCAGTC
    AGCAAGTCTG AGCTGGAAGA TGACACTGAG AGCCCTGACT ACGCTGAGGA ATCAGGAGCT
    GAAGTCTTCA CCTTGCCAGA GAGGAAGATG AAACCTCCCA TAGCCGAGAA GCCCCCTCTG
    GCCCGAAGGC CTCCAAGCTT AGTCCACAAG CCACCATCTG TCCCCGAGGA GTACCCACTA
    ACTTCGCCTA CATTGGCTAT GACTCCCAAG AGCTCAATTC AGCACATAAG GCCACTCCCT
    CAAGATATCT ACACAGTGGT GCGGAAATCA AAGTCCTCCA GCTTCCCTGA GAGCAGAAGC
    CCAGGGGAGT CCACAGCACC CTCATCTCTT GTTTTCACGC CTTTTGCCAG TTCCTCTGGT
    GCTTTCTTCT CAGGAACACA GCAACCTCCC CAGGGAAGTA TGGAGGATGA GGGCCCTAAG
    GGGAGAGCCC TGCCTGAAAG AATTAGCCTC CAGAGCCAAG AAGAAGCTGA GAAAAAGAAA
    GGCAAGATTC CACCTCCTGT ACCAAAAAAG CCCAGCGTGC TGTACCTGCC TCTCACTTCA
    CCCATGGCTC AAGCGGAGGC CTATGTGACT GAACCAAGAC TGCCCCTCAG CCCCATCATC
    ACCCTGGAGG AAGATGCCAA GTGTCTCCCA ACTGATGACC ACCTGCCATC TGCTGGTACA
    AGGATGACCT CAAGGCCACA GTCCAACAGC GAAAGGGAAG CAAGTCCTCC AGGGAGTTTG
    ATGGAACCAA GCACCGAAGA AAAAAGTGTA ATCAGTGATA AAACAGCCGA ATGGATTGCA
    GAAGATGATG ATGATGTGTT TGTGGCTTCA CGTACAACTG AAGATTTATT TACTGTGATC
    CACAGGTCCA AAAGAAAGCT GCTTGGCTGG AAGGAACCTG GTGAGACCTT TGCTGGCAGC
    AGCAGACCAA GCTCCCACTC ACCAGTAAGG AACCCACCTG AGTCTCCGGT CAGTGAGTTG
    GCTGCCTCTT CAGGGTCAAG CGGCAGTGCC AACCTAGATG CTGGCAGAAA TGATGATTTC
    AAGGCCTTGC TACAGAAGAA GGGAAGCAAG GCAACTCCAA GGTCCCGCCC CTCAGCAGCC
    GAACTGCTGA AGACCACTAA CCCACTGGCT CGGCGAATTA TTGCACAATT TTCAAAAGAC
    TACAAAACCC CTGATAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238678
    Clone ID Related Accession (Same CDS sequence) XM_024988571.1
    Accession Version XM_024988571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGGGCAGC AGTTTGATAA ACATGCAAGT TTGCGACACT CGTTGTTTAA CACAGAGACA
    GCCGTGAACC CCAAGTCCAC CCTGAGGCGG AGGCGGACCA TTATTGGATT CTCTAACTAT
    TCCCAGCGAG ACCAAGGTCA CAGCAACAGC CCAGCAGGCA GTGTGGCCCG CTCTACCACC
    TCAGACATCA GGCCCAGCCA TTCAGTTCCA GAAAGTGTTC ATGGAAGAGT TGCACTTGGT
    CAGGAAGCTC GGTTCCCAAA TCTCACCTCA CCAGTACTAA GAAATCCTTC TAGTCATCCG
    GAAGAACCTC CCCAGGCATG GGGTGCCACA AAACCCCCTG GAATGGAGAG CATGGGGATG
    GTGTACAGCA TTCCTGGTTC TTGCAATGGA CCAACAGAAT CGACATTCTC TGCTTCCAGG
    AAGGGAGATG CTTTTACCTA TGTGACTCCA AGTGCCACCG CTCAGAGCAG TCAAGTCAAT
    GAAAATGGAA AAAGTCCTTC CTCTGGGAAT TCTTGGGTCT CTCTGCACAC ACTGCCACCT
    CTTGTTCCTA AGGAGGCTGC TACCCTCTTT GTCACTCGTG ATATCCCAGC AGGGTGCAGT
    GGGCCTGCTG GCTACTCTCA GCATCCTGCT CAACGAAGCC AAGTATCTGA ACGACCCTCC
    AAGATTGGCC TTCTGACCGG TGGTACCTCA AGGCTGGAGA CAGGCCCAGG TGGGGCCAGC
    AAGTTCCGGG AGCGGTCACT GTCTGTACCC ACAGATACAG GTACCATGGA TGTGGACTAT
    GAGGAGGAGC AGAAGGCCAG TGAAGCCTGT GCCCTACCTT ATGCCAGTAC GAGTTCTGAG
    GGCAGTAACA GTGCTGACAA CATTGCCTCC CTCAGTGCCC AACAGGAGGC CCATCACAGA
    AGGCAGAGGT CCAAGAGCAT CTCACTCAAG AAGGCCAAGA AGAAGCCTTC CCCTCCGACT
    CGCAGTGTCT CACTGGTCAA AGATGAACCA GTCCTCTTGC CGGAAGGTGG GTCAGTACTA
    CCCAAGGACC AGAGGCCCAG GAGCCTTTGT CTCTCCTTGG AACACCAAGG ACATCACTCA
    TCCCACCCAG ATACTCAGGG TCACCCAGCT GTGCCAACCT TCAAAGACCC AGAAGGTACA
    CAATTCGCCC ATCACTGGTA TCTTACTGAC TGGAAGTCTG GTGACACCTA CCAGTCCTTG
    TCCAGCTCCA GCACTGCCAC TGGCACCACA GTCATTGAGT GCACCCAAGT TCAGGGCAGC
    TCAGAGTCTC TTGCCTCCCC TTCCACTTCC AGAGCCACGA CGCCTTCCCA GCTTTCCATC
    GAGGTGGAGG CCAGGGAGGT ATCCTCTCCA GGAAGGCCTA CTGGGCTGAT GTCACCCTCC
    AGTGGATACT CCAGCCAGTC AGAGACGCCA ACACCCACTG TCTCCATGTC CTTGACCCTG
    GGCCACTTGC CCCCTCCAAG CAGCAGTGTC CGGGTGCGTC CAGTGGTACC TGAGAGGAAG
    TCATCACTAC CCCCAACATC ACCAATGGAG AAAATGTCCA AGTCACGGCT ATCGTTTGAC
    CTACCATTGA CCTCTTCAAC CAACCTGGAT CTGTCTGGGA TGAGTATCTC AATCCGAAGC
    AAAAACAAGG TGAGCCAGCA TCACTCAGAT ACAAATTTGG GGGCCAAGCT AGCCCAGAAA
    ACTAGCCCCA ACCAGCCAAT CATGCCCATG GTTACTCAGT CTGACTTACG TTCTGTTCGC
    CTGAGGTCAG TCAGCAAGTC TGAGCTGGAA GATGACACTG AGAGCCCTGA CTACGCTGAG
    GAATCAGGAG CTGAAGTCTT CACCTTGCCA GAGAGGAAGA TGAAACCTCC CATAGCCGAG
    AAGCCCCCTC TGGCCCGAAG GCCTCCAAGC TTAGTCCACA AGCCACCATC TGTCCCCGAG
    GAGTACCCAC TAACTTCGCC TACATTGGCT ATGACTCCCA AGAGCTCAAT TCAGCACATA
    AGGCCACTCC CTCAAGATAT CTACACAGTG GTGCGGAAAT CAAAGTCCTC CAGCTTCCCT
    GAGAGCAGAA GCCCAGGGGA GTCCACAGCA CCCTCATCTC TTGTTTTCAC GCCTTTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGGAAG TATGGAGGAT
    GAGGGCCCTA AGGGGAGAGC CCTGCCTGAA AGAATTAGCC TCCAGAGCCA AGAAGAAGCT
    GAGAAAAAGA AAGGCAAGAT TCCACCTCCT GTACCAAAAA AGCCCAGCGT GCTGTACCTG
    CCTCTCACTT CACCCATGGC TCAAGCGGAG GCCTATGTGA CTGAACCAAG ACTGCCCCTC
    AGCCCCATCA TCACCCTGGA GGAAGATGCC AAGTGTCTCC CAACTGATGA CCACCTGCCA
    TCTGCTGGTA CAAGGATGAC CTCAAGGCCA CAGTCCAACA GCGAAAGGGA AGCAAGTCCT
    CCAGGGAGTT TGATGGAACC AAGCACCGAA GAAAAAAGTG TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAAGATGA TGATGATGTG TTTGTGGCTT CACGTACAAC TGAAGATTTA
    TTTACTGTGA TCCACAGGTC CAAAAGAAAG CTGCTTGGCT GGAAGGAACC TGGTGAGACC
    TTTGCTGGCA GCAGCAGACC AAGCTCCCAC TCACCAGTAA GGAACCCACC TGAGTCTCCG
    GTCAGTGAGT TGGCTGCCTC TTCAGGGTCA AGCGGCAGTG CCAACCTAGA TGCTGGCAGA
    AATGATGATT TCAAGGCCTT GCTACAGAAG AAGGGAAGCA AGGCAACTCC AAGGTCCCGC
    CCCTCAGCAG CCGAACTGCT GAAGACCACT AACCCACTGG CTCGGCGAAT TATTGCACAA
    TTTTCAAAAG ACTACAAAAC CCCTGATAAC CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844339.1
    CDS75..3176
    Translation

    Target ORF information:

    RefSeq Version XM_024988571.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGGGCAGC AGTTTGATAA ACATGCAAGT TTGCGACACT CGTTGTTTAA CACAGAGACA
    GCCGTGAACC CCAAGTCCAC CCTGAGGCGG AGGCGGACCA TTATTGGATT CTCTAACTAT
    TCCCAGCGAG ACCAAGGTCA CAGCAACAGC CCAGCAGGCA GTGTGGCCCG CTCTACCACC
    TCAGACATCA GGCCCAGCCA TTCAGTTCCA GAAAGTGTTC ATGGAAGAGT TGCACTTGGT
    CAGGAAGCTC GGTTCCCAAA TCTCACCTCA CCAGTACTAA GAAATCCTTC TAGTCATCCG
    GAAGAACCTC CCCAGGCATG GGGTGCCACA AAACCCCCTG GAATGGAGAG CATGGGGATG
    GTGTACAGCA TTCCTGGTTC TTGCAATGGA CCAACAGAAT CGACATTCTC TGCTTCCAGG
    AAGGGAGATG CTTTTACCTA TGTGACTCCA AGTGCCACCG CTCAGAGCAG TCAAGTCAAT
    GAAAATGGAA AAAGTCCTTC CTCTGGGAAT TCTTGGGTCT CTCTGCACAC ACTGCCACCT
    CTTGTTCCTA AGGAGGCTGC TACCCTCTTT GTCACTCGTG ATATCCCAGC AGGGTGCAGT
    GGGCCTGCTG GCTACTCTCA GCATCCTGCT CAACGAAGCC AAGTATCTGA ACGACCCTCC
    AAGATTGGCC TTCTGACCGG TGGTACCTCA AGGCTGGAGA CAGGCCCAGG TGGGGCCAGC
    AAGTTCCGGG AGCGGTCACT GTCTGTACCC ACAGATACAG GTACCATGGA TGTGGACTAT
    GAGGAGGAGC AGAAGGCCAG TGAAGCCTGT GCCCTACCTT ATGCCAGTAC GAGTTCTGAG
    GGCAGTAACA GTGCTGACAA CATTGCCTCC CTCAGTGCCC AACAGGAGGC CCATCACAGA
    AGGCAGAGGT CCAAGAGCAT CTCACTCAAG AAGGCCAAGA AGAAGCCTTC CCCTCCGACT
    CGCAGTGTCT CACTGGTCAA AGATGAACCA GTCCTCTTGC CGGAAGGTGG GTCAGTACTA
    CCCAAGGACC AGAGGCCCAG GAGCCTTTGT CTCTCCTTGG AACACCAAGG ACATCACTCA
    TCCCACCCAG ATACTCAGGG TCACCCAGCT GTGCCAACCT TCAAAGACCC AGAAGGTACA
    CAATTCGCCC ATCACTGGTA TCTTACTGAC TGGAAGTCTG GTGACACCTA CCAGTCCTTG
    TCCAGCTCCA GCACTGCCAC TGGCACCACA GTCATTGAGT GCACCCAAGT TCAGGGCAGC
    TCAGAGTCTC TTGCCTCCCC TTCCACTTCC AGAGCCACGA CGCCTTCCCA GCTTTCCATC
    GAGGTGGAGG CCAGGGAGGT ATCCTCTCCA GGAAGGCCTA CTGGGCTGAT GTCACCCTCC
    AGTGGATACT CCAGCCAGTC AGAGACGCCA ACACCCACTG TCTCCATGTC CTTGACCCTG
    GGCCACTTGC CCCCTCCAAG CAGCAGTGTC CGGGTGCGTC CAGTGGTACC TGAGAGGAAG
    TCATCACTAC CCCCAACATC ACCAATGGAG AAAATGTCCA AGTCACGGCT ATCGTTTGAC
    CTACCATTGA CCTCTTCAAC CAACCTGGAT CTGTCTGGGA TGAGTATCTC AATCCGAAGC
    AAAAACAAGG TGAGCCAGCA TCACTCAGAT ACAAATTTGG GGGCCAAGCT AGCCCAGAAA
    ACTAGCCCCA ACCAGCCAAT CATGCCCATG GTTACTCAGT CTGACTTACG TTCTGTTCGC
    CTGAGGTCAG TCAGCAAGTC TGAGCTGGAA GATGACACTG AGAGCCCTGA CTACGCTGAG
    GAATCAGGAG CTGAAGTCTT CACCTTGCCA GAGAGGAAGA TGAAACCTCC CATAGCCGAG
    AAGCCCCCTC TGGCCCGAAG GCCTCCAAGC TTAGTCCACA AGCCACCATC TGTCCCCGAG
    GAGTACCCAC TAACTTCGCC TACATTGGCT ATGACTCCCA AGAGCTCAAT TCAGCACATA
    AGGCCACTCC CTCAAGATAT CTACACAGTG GTGCGGAAAT CAAAGTCCTC CAGCTTCCCT
    GAGAGCAGAA GCCCAGGGGA GTCCACAGCA CCCTCATCTC TTGTTTTCAC GCCTTTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGGAAG TATGGAGGAT
    GAGGGCCCTA AGGGGAGAGC CCTGCCTGAA AGAATTAGCC TCCAGAGCCA AGAAGAAGCT
    GAGAAAAAGA AAGGCAAGAT TCCACCTCCT GTACCAAAAA AGCCCAGCGT GCTGTACCTG
    CCTCTCACTT CACCCATGGC TCAAGCGGAG GCCTATGTGA CTGAACCAAG ACTGCCCCTC
    AGCCCCATCA TCACCCTGGA GGAAGATGCC AAGTGTCTCC CAACTGATGA CCACCTGCCA
    TCTGCTGGTA CAAGGATGAC CTCAAGGCCA CAGTCCAACA GCGAAAGGGA AGCAAGTCCT
    CCAGGGAGTT TGATGGAACC AAGCACCGAA GAAAAAAGTG TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAAGATGA TGATGATGTG TTTGTGGCTT CACGTACAAC TGAAGATTTA
    TTTACTGTGA TCCACAGGTC CAAAAGAAAG CTGCTTGGCT GGAAGGAACC TGGTGAGACC
    TTTGCTGGCA GCAGCAGACC AAGCTCCCAC TCACCAGTAA GGAACCCACC TGAGTCTCCG
    GTCAGTGAGT TGGCTGCCTC TTCAGGGTCA AGCGGCAGTG CCAACCTAGA TGCTGGCAGA
    AATGATGATT TCAAGGCCTT GCTACAGAAG AAGGGAAGCA AGGCAACTCC AAGGTCCCGC
    CCCTCAGCAG CCGAACTGCT GAAGACCACT AACCCACTGG CTCGGCGAAT TATTGCACAA
    TTTTCAAAAG ACTACAAAAC CCCTGATAAC CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89354
    Clone ID Related Accession (Same CDS sequence) XM_015461642.1 , XM_015470420.1
    Accession Version XM_015461642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG CTGGAAAGTC TTTGGCAGCC
    ATCTGGACAA TTCAAGGGCA CCCTGTGCCT CACTGTGTGG TGATGGGTCA CAGCAACAGC
    CCAGCAGGCA GTGTGGCCCG CTCTACCACC TCAGACATCA GGCCCAGCCA TTCAGTTCCA
    GAAAGTGTTC ATGGAAGAGT TGCACTTGGT CAGGAAGCTC GGTTCCCAAA TCTCACCTCA
    CCAGTACTAA GAAATCCTTC TAGTCATCCG GAAGAACCTC CCCAGGCATG GGGTGCCACA
    AAACCCCCTG GAATGGAGAG CATGGGGATG GTGTACAGCA TTCCTGGTTC TTGCAATGGA
    CCAACAGAAT CGACATTCTC TGCTTCCAGG AAGGGAGATG CTTTTACCTA TGTGACTCCA
    AGTGCCACCG CTCAGAGCAG TCAAGTCAAT GAAAATGGAA AAAGTCCTTC CTCTGGGAAT
    TCTTGGGTCT CTCTGCACAC ACTGCCACCT CTTGTTCCTA AGGAGGCTGC TACCCTCTTT
    GTCACTCGTG ATATCCCAGC AGGGTGCAGT GGGCCTGCTG GCTACTCTCA GCATCCTGCT
    CAACGAAGCC AAGTATCTGA ACGACCCTCC AAGATTGGCC TTCTGACCGG TGGTACCTCA
    AGGCTGGAGA CAGGCCCAGG TGGGGCCAGC AAGTTCCGGG AGCGGTCACT GTCTGTACCC
    ACAGATACAG GTACCATGGA TGTGGACTAT GAGGAGGAGC AGAAGGCCAG TGAAGCCTGT
    GCCCTACCTT ATGCCAGTAC GAGTTCTGAG GGCAGTAACA GTGCTGACAA CATTGCCTCC
    CTCAGTGCCC AACAGGAGGC CCATCACAGA AGGCAGAGGT CCAAGAGCAT CTCACTCAAG
    AAGGCCAAGA AGAAGCCTTC CCCTCCGACT CGCAGTGTCT CACTGGTCAA AGATGAACCA
    GTCCTCTTGC CGGAAGGTGG GTCAGTACTA CCCAAGGACC AGAGGCCCAG GAGCCTTTGT
    CTCTCCTTGG AACACCAAGG ACATCACTCA TCCCACCCAG ATACTCAGGG TCACCCAGCT
    GTGCCAACCT TCAAAGACCC AGAAGGTACA CAATTCGCCC ATCACTGGTA TCTTACTGAC
    TGGAAGTCTG GTGACACCTA CCAGTCCTTG TCCAGCTCCA GCACTGCCAC TGGCACCACA
    GTCATTGAGT GCACCCAAGT TCAGGGCAGC TCAGAGTCTC TTGCCTCCCC TTCCACTTCC
    AGAGCCACGA CGCCTTCCCA GCTTTCCATC GAGGTGGAGG CCAGGGAGGT ATCCTCTCCA
    GGAAGGCCTA CTGGGCTGAT GTCACCCTCC AGTGGATACT CCAGCCAGTC AGAGACGCCA
    ACACCCACTG TCTCCATGTC CTTGACCCTG GGCCACTTGC CCCCTCCAAG CAGCAGTGTC
    CGGGTGCGTC CAGTGGTACC TGAGAGGAAG TCATCACTAC CCCCAACATC ACCAATGGAG
    AAAATGTCCA AGTCACGGCT ATCGTTTGAC CTACCATTGA CCTCTTCAAC CAACCTGGAT
    CTGTCTGGGA TGAGTATCTC AATCCGAAGC AAAAACAAGG TGAGCCAGCA TCACTCAGAT
    ACAAATTTGG GGGCCAAGCT AGCCCAGAAA ACTAGCCCCA ACCAGCCAAT CATGCCCATG
    GTTACTCAGT CTGACTTACG TTCTGTTCGC CTGAGGTCAG TCAGCAAGTC TGAGCTGGAA
    GATGACACTG AGAGCCCTGA CTACGCTGAG GAATCAGGAG CTGAAGTCTT CACCTTGCCA
    GAGAGGAAGA TGAAACCTCC CATAGCCGAG AAGCCCCCTC TGGCCCGAAG GCCTCCAAGC
    TTAGTCCACA AGCCACCATC TGTCCCCGAG GAGTACCCAC TAACTTCGCC TACATTGGCT
    ATGACTCCCA AGAGCTCAAT TCAGCACATA AGGCCACTCC CTCAAGATAT CTACACAGTG
    GTGCGGAAAT CAAAGTCCTC CAGCTTCCCT GAGAGCAGAA GCCCAGGGGA GTCCACAGCA
    CCCTCATCTC TTGTTTTCAC GCCTTTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGGAAG TATGGAGGAT GAGGGCCCTA AGGGGAGAGC CCTGCCTGAA
    AGAATTAGCC TCCAGAGCCA AGAAGAAGCT GAGAAAAAGA AAGGCAAGAT TCCACCTCCT
    GTACCAAAAA AGCCCAGCGT GCTGTACCTG CCTCTCACTT CACCCATGGC TCAAGCGGAG
    GCCTATGTGA CTGAACCAAG ACTGCCCCTC AGCCCCATCA TCACCCTGGA GGAAGATGCC
    AAGTGTCTCC CAACTGATGA CCACCTGCCA TCTGCTGGTA CAAGGATGAC CTCAAGGCCA
    CAGTCCAACA GCGAAAGGGA AGCAAGTCCT CCAGGGAGTT TGATGGAACC AAGCACCGAA
    GAAAAAAGTG TAATCAGTGA TAAAACAGCC GAATGGATTG CAGAAGATGA TGATGATGTG
    TTTGTGGCTT CACGTACAAC TGAAGATTTA TTTACTGTGA TCCACAGGTC CAAAAGAAAG
    CTGCTTGGCT GGAAGGAACC TGGTGAGACC TTTGCTGGCA GCAGCAGACC AAGCTCCCAC
    TCACCAGTAA GGAACCCACC TGAGTCTCCG GTCAGTGAGT TGGCTGCCTC TTCAGGGTCA
    AGCGGCAGTG CCAACCTAGA TGCTGGCAGA AATGATGATT TCAAGGCCTT GCTACAGAAG
    AAGGGAAGCA AGGCAACTCC AAGGTCCCGC CCCTCAGCAG CCGAACTGCT GAAGACCACT
    AACCCACTGG CTCGGCGAAT TATTGCACAA TTTTCAAAAG ACTACAAAAC CCCTGATAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317128.1
    CDS863..4354
    Translation

    Target ORF information:

    RefSeq Version XM_015461642.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG CTGGAAAGTC TTTGGCAGCC
    ATCTGGACAA TTCAAGGGCA CCCTGTGCCT CACTGTGTGG TGATGGGTCA CAGCAACAGC
    CCAGCAGGCA GTGTGGCCCG CTCTACCACC TCAGACATCA GGCCCAGCCA TTCAGTTCCA
    GAAAGTGTTC ATGGAAGAGT TGCACTTGGT CAGGAAGCTC GGTTCCCAAA TCTCACCTCA
    CCAGTACTAA GAAATCCTTC TAGTCATCCG GAAGAACCTC CCCAGGCATG GGGTGCCACA
    AAACCCCCTG GAATGGAGAG CATGGGGATG GTGTACAGCA TTCCTGGTTC TTGCAATGGA
    CCAACAGAAT CGACATTCTC TGCTTCCAGG AAGGGAGATG CTTTTACCTA TGTGACTCCA
    AGTGCCACCG CTCAGAGCAG TCAAGTCAAT GAAAATGGAA AAAGTCCTTC CTCTGGGAAT
    TCTTGGGTCT CTCTGCACAC ACTGCCACCT CTTGTTCCTA AGGAGGCTGC TACCCTCTTT
    GTCACTCGTG ATATCCCAGC AGGGTGCAGT GGGCCTGCTG GCTACTCTCA GCATCCTGCT
    CAACGAAGCC AAGTATCTGA ACGACCCTCC AAGATTGGCC TTCTGACCGG TGGTACCTCA
    AGGCTGGAGA CAGGCCCAGG TGGGGCCAGC AAGTTCCGGG AGCGGTCACT GTCTGTACCC
    ACAGATACAG GTACCATGGA TGTGGACTAT GAGGAGGAGC AGAAGGCCAG TGAAGCCTGT
    GCCCTACCTT ATGCCAGTAC GAGTTCTGAG GGCAGTAACA GTGCTGACAA CATTGCCTCC
    CTCAGTGCCC AACAGGAGGC CCATCACAGA AGGCAGAGGT CCAAGAGCAT CTCACTCAAG
    AAGGCCAAGA AGAAGCCTTC CCCTCCGACT CGCAGTGTCT CACTGGTCAA AGATGAACCA
    GTCCTCTTGC CGGAAGGTGG GTCAGTACTA CCCAAGGACC AGAGGCCCAG GAGCCTTTGT
    CTCTCCTTGG AACACCAAGG ACATCACTCA TCCCACCCAG ATACTCAGGG TCACCCAGCT
    GTGCCAACCT TCAAAGACCC AGAAGGTACA CAATTCGCCC ATCACTGGTA TCTTACTGAC
    TGGAAGTCTG GTGACACCTA CCAGTCCTTG TCCAGCTCCA GCACTGCCAC TGGCACCACA
    GTCATTGAGT GCACCCAAGT TCAGGGCAGC TCAGAGTCTC TTGCCTCCCC TTCCACTTCC
    AGAGCCACGA CGCCTTCCCA GCTTTCCATC GAGGTGGAGG CCAGGGAGGT ATCCTCTCCA
    GGAAGGCCTA CTGGGCTGAT GTCACCCTCC AGTGGATACT CCAGCCAGTC AGAGACGCCA
    ACACCCACTG TCTCCATGTC CTTGACCCTG GGCCACTTGC CCCCTCCAAG CAGCAGTGTC
    CGGGTGCGTC CAGTGGTACC TGAGAGGAAG TCATCACTAC CCCCAACATC ACCAATGGAG
    AAAATGTCCA AGTCACGGCT ATCGTTTGAC CTACCATTGA CCTCTTCAAC CAACCTGGAT
    CTGTCTGGGA TGAGTATCTC AATCCGAAGC AAAAACAAGG TGAGCCAGCA TCACTCAGAT
    ACAAATTTGG GGGCCAAGCT AGCCCAGAAA ACTAGCCCCA ACCAGCCAAT CATGCCCATG
    GTTACTCAGT CTGACTTACG TTCTGTTCGC CTGAGGTCAG TCAGCAAGTC TGAGCTGGAA
    GATGACACTG AGAGCCCTGA CTACGCTGAG GAATCAGGAG CTGAAGTCTT CACCTTGCCA
    GAGAGGAAGA TGAAACCTCC CATAGCCGAG AAGCCCCCTC TGGCCCGAAG GCCTCCAAGC
    TTAGTCCACA AGCCACCATC TGTCCCCGAG GAGTACCCAC TAACTTCGCC TACATTGGCT
    ATGACTCCCA AGAGCTCAAT TCAGCACATA AGGCCACTCC CTCAAGATAT CTACACAGTG
    GTGCGGAAAT CAAAGTCCTC CAGCTTCCCT GAGAGCAGAA GCCCAGGGGA GTCCACAGCA
    CCCTCATCTC TTGTTTTCAC GCCTTTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGGAAG TATGGAGGAT GAGGGCCCTA AGGGGAGAGC CCTGCCTGAA
    AGAATTAGCC TCCAGAGCCA AGAAGAAGCT GAGAAAAAGA AAGGCAAGAT TCCACCTCCT
    GTACCAAAAA AGCCCAGCGT GCTGTACCTG CCTCTCACTT CACCCATGGC TCAAGCGGAG
    GCCTATGTGA CTGAACCAAG ACTGCCCCTC AGCCCCATCA TCACCCTGGA GGAAGATGCC
    AAGTGTCTCC CAACTGATGA CCACCTGCCA TCTGCTGGTA CAAGGATGAC CTCAAGGCCA
    CAGTCCAACA GCGAAAGGGA AGCAAGTCCT CCAGGGAGTT TGATGGAACC AAGCACCGAA
    GAAAAAAGTG TAATCAGTGA TAAAACAGCC GAATGGATTG CAGAAGATGA TGATGATGTG
    TTTGTGGCTT CACGTACAAC TGAAGATTTA TTTACTGTGA TCCACAGGTC CAAAAGAAAG
    CTGCTTGGCT GGAAGGAACC TGGTGAGACC TTTGCTGGCA GCAGCAGACC AAGCTCCCAC
    TCACCAGTAA GGAACCCACC TGAGTCTCCG GTCAGTGAGT TGGCTGCCTC TTCAGGGTCA
    AGCGGCAGTG CCAACCTAGA TGCTGGCAGA AATGATGATT TCAAGGCCTT GCTACAGAAG
    AAGGGAAGCA AGGCAACTCC AAGGTCCCGC CCCTCAGCAG CCGAACTGCT GAAGACCACT
    AACCCACTGG CTCGGCGAAT TATTGCACAA TTTTCAAAAG ACTACAAAAC CCCTGATAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89354
    Clone ID Related Accession (Same CDS sequence) XM_015461642.1 , XM_015470420.1
    Accession Version XM_015470420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG CTGGAAAGTC TTTGGCAGCC
    ATCTGGACAA TTCAAGGGCA CCCTGTGCCT CACTGTGTGG TGATGGGTCA CAGCAACAGC
    CCAGCAGGCA GTGTGGCCCG CTCTACCACC TCAGACATCA GGCCCAGCCA TTCAGTTCCA
    GAAAGTGTTC ATGGAAGAGT TGCACTTGGT CAGGAAGCTC GGTTCCCAAA TCTCACCTCA
    CCAGTACTAA GAAATCCTTC TAGTCATCCG GAAGAACCTC CCCAGGCATG GGGTGCCACA
    AAACCCCCTG GAATGGAGAG CATGGGGATG GTGTACAGCA TTCCTGGTTC TTGCAATGGA
    CCAACAGAAT CGACATTCTC TGCTTCCAGG AAGGGAGATG CTTTTACCTA TGTGACTCCA
    AGTGCCACCG CTCAGAGCAG TCAAGTCAAT GAAAATGGAA AAAGTCCTTC CTCTGGGAAT
    TCTTGGGTCT CTCTGCACAC ACTGCCACCT CTTGTTCCTA AGGAGGCTGC TACCCTCTTT
    GTCACTCGTG ATATCCCAGC AGGGTGCAGT GGGCCTGCTG GCTACTCTCA GCATCCTGCT
    CAACGAAGCC AAGTATCTGA ACGACCCTCC AAGATTGGCC TTCTGACCGG TGGTACCTCA
    AGGCTGGAGA CAGGCCCAGG TGGGGCCAGC AAGTTCCGGG AGCGGTCACT GTCTGTACCC
    ACAGATACAG GTACCATGGA TGTGGACTAT GAGGAGGAGC AGAAGGCCAG TGAAGCCTGT
    GCCCTACCTT ATGCCAGTAC GAGTTCTGAG GGCAGTAACA GTGCTGACAA CATTGCCTCC
    CTCAGTGCCC AACAGGAGGC CCATCACAGA AGGCAGAGGT CCAAGAGCAT CTCACTCAAG
    AAGGCCAAGA AGAAGCCTTC CCCTCCGACT CGCAGTGTCT CACTGGTCAA AGATGAACCA
    GTCCTCTTGC CGGAAGGTGG GTCAGTACTA CCCAAGGACC AGAGGCCCAG GAGCCTTTGT
    CTCTCCTTGG AACACCAAGG ACATCACTCA TCCCACCCAG ATACTCAGGG TCACCCAGCT
    GTGCCAACCT TCAAAGACCC AGAAGGTACA CAATTCGCCC ATCACTGGTA TCTTACTGAC
    TGGAAGTCTG GTGACACCTA CCAGTCCTTG TCCAGCTCCA GCACTGCCAC TGGCACCACA
    GTCATTGAGT GCACCCAAGT TCAGGGCAGC TCAGAGTCTC TTGCCTCCCC TTCCACTTCC
    AGAGCCACGA CGCCTTCCCA GCTTTCCATC GAGGTGGAGG CCAGGGAGGT ATCCTCTCCA
    GGAAGGCCTA CTGGGCTGAT GTCACCCTCC AGTGGATACT CCAGCCAGTC AGAGACGCCA
    ACACCCACTG TCTCCATGTC CTTGACCCTG GGCCACTTGC CCCCTCCAAG CAGCAGTGTC
    CGGGTGCGTC CAGTGGTACC TGAGAGGAAG TCATCACTAC CCCCAACATC ACCAATGGAG
    AAAATGTCCA AGTCACGGCT ATCGTTTGAC CTACCATTGA CCTCTTCAAC CAACCTGGAT
    CTGTCTGGGA TGAGTATCTC AATCCGAAGC AAAAACAAGG TGAGCCAGCA TCACTCAGAT
    ACAAATTTGG GGGCCAAGCT AGCCCAGAAA ACTAGCCCCA ACCAGCCAAT CATGCCCATG
    GTTACTCAGT CTGACTTACG TTCTGTTCGC CTGAGGTCAG TCAGCAAGTC TGAGCTGGAA
    GATGACACTG AGAGCCCTGA CTACGCTGAG GAATCAGGAG CTGAAGTCTT CACCTTGCCA
    GAGAGGAAGA TGAAACCTCC CATAGCCGAG AAGCCCCCTC TGGCCCGAAG GCCTCCAAGC
    TTAGTCCACA AGCCACCATC TGTCCCCGAG GAGTACCCAC TAACTTCGCC TACATTGGCT
    ATGACTCCCA AGAGCTCAAT TCAGCACATA AGGCCACTCC CTCAAGATAT CTACACAGTG
    GTGCGGAAAT CAAAGTCCTC CAGCTTCCCT GAGAGCAGAA GCCCAGGGGA GTCCACAGCA
    CCCTCATCTC TTGTTTTCAC GCCTTTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGGAAG TATGGAGGAT GAGGGCCCTA AGGGGAGAGC CCTGCCTGAA
    AGAATTAGCC TCCAGAGCCA AGAAGAAGCT GAGAAAAAGA AAGGCAAGAT TCCACCTCCT
    GTACCAAAAA AGCCCAGCGT GCTGTACCTG CCTCTCACTT CACCCATGGC TCAAGCGGAG
    GCCTATGTGA CTGAACCAAG ACTGCCCCTC AGCCCCATCA TCACCCTGGA GGAAGATGCC
    AAGTGTCTCC CAACTGATGA CCACCTGCCA TCTGCTGGTA CAAGGATGAC CTCAAGGCCA
    CAGTCCAACA GCGAAAGGGA AGCAAGTCCT CCAGGGAGTT TGATGGAACC AAGCACCGAA
    GAAAAAAGTG TAATCAGTGA TAAAACAGCC GAATGGATTG CAGAAGATGA TGATGATGTG
    TTTGTGGCTT CACGTACAAC TGAAGATTTA TTTACTGTGA TCCACAGGTC CAAAAGAAAG
    CTGCTTGGCT GGAAGGAACC TGGTGAGACC TTTGCTGGCA GCAGCAGACC AAGCTCCCAC
    TCACCAGTAA GGAACCCACC TGAGTCTCCG GTCAGTGAGT TGGCTGCCTC TTCAGGGTCA
    AGCGGCAGTG CCAACCTAGA TGCTGGCAGA AATGATGATT TCAAGGCCTT GCTACAGAAG
    AAGGGAAGCA AGGCAACTCC AAGGTCCCGC CCCTCAGCAG CCGAACTGCT GAAGACCACT
    AACCCACTGG CTCGGCGAAT TATTGCACAA TTTTCAAAAG ACTACAAAAC CCCTGATAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325906.1
    CDS863..4354
    Translation

    Target ORF information:

    RefSeq Version XM_015470420.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG CTGGAAAGTC TTTGGCAGCC
    ATCTGGACAA TTCAAGGGCA CCCTGTGCCT CACTGTGTGG TGATGGGTCA CAGCAACAGC
    CCAGCAGGCA GTGTGGCCCG CTCTACCACC TCAGACATCA GGCCCAGCCA TTCAGTTCCA
    GAAAGTGTTC ATGGAAGAGT TGCACTTGGT CAGGAAGCTC GGTTCCCAAA TCTCACCTCA
    CCAGTACTAA GAAATCCTTC TAGTCATCCG GAAGAACCTC CCCAGGCATG GGGTGCCACA
    AAACCCCCTG GAATGGAGAG CATGGGGATG GTGTACAGCA TTCCTGGTTC TTGCAATGGA
    CCAACAGAAT CGACATTCTC TGCTTCCAGG AAGGGAGATG CTTTTACCTA TGTGACTCCA
    AGTGCCACCG CTCAGAGCAG TCAAGTCAAT GAAAATGGAA AAAGTCCTTC CTCTGGGAAT
    TCTTGGGTCT CTCTGCACAC ACTGCCACCT CTTGTTCCTA AGGAGGCTGC TACCCTCTTT
    GTCACTCGTG ATATCCCAGC AGGGTGCAGT GGGCCTGCTG GCTACTCTCA GCATCCTGCT
    CAACGAAGCC AAGTATCTGA ACGACCCTCC AAGATTGGCC TTCTGACCGG TGGTACCTCA
    AGGCTGGAGA CAGGCCCAGG TGGGGCCAGC AAGTTCCGGG AGCGGTCACT GTCTGTACCC
    ACAGATACAG GTACCATGGA TGTGGACTAT GAGGAGGAGC AGAAGGCCAG TGAAGCCTGT
    GCCCTACCTT ATGCCAGTAC GAGTTCTGAG GGCAGTAACA GTGCTGACAA CATTGCCTCC
    CTCAGTGCCC AACAGGAGGC CCATCACAGA AGGCAGAGGT CCAAGAGCAT CTCACTCAAG
    AAGGCCAAGA AGAAGCCTTC CCCTCCGACT CGCAGTGTCT CACTGGTCAA AGATGAACCA
    GTCCTCTTGC CGGAAGGTGG GTCAGTACTA CCCAAGGACC AGAGGCCCAG GAGCCTTTGT
    CTCTCCTTGG AACACCAAGG ACATCACTCA TCCCACCCAG ATACTCAGGG TCACCCAGCT
    GTGCCAACCT TCAAAGACCC AGAAGGTACA CAATTCGCCC ATCACTGGTA TCTTACTGAC
    TGGAAGTCTG GTGACACCTA CCAGTCCTTG TCCAGCTCCA GCACTGCCAC TGGCACCACA
    GTCATTGAGT GCACCCAAGT TCAGGGCAGC TCAGAGTCTC TTGCCTCCCC TTCCACTTCC
    AGAGCCACGA CGCCTTCCCA GCTTTCCATC GAGGTGGAGG CCAGGGAGGT ATCCTCTCCA
    GGAAGGCCTA CTGGGCTGAT GTCACCCTCC AGTGGATACT CCAGCCAGTC AGAGACGCCA
    ACACCCACTG TCTCCATGTC CTTGACCCTG GGCCACTTGC CCCCTCCAAG CAGCAGTGTC
    CGGGTGCGTC CAGTGGTACC TGAGAGGAAG TCATCACTAC CCCCAACATC ACCAATGGAG
    AAAATGTCCA AGTCACGGCT ATCGTTTGAC CTACCATTGA CCTCTTCAAC CAACCTGGAT
    CTGTCTGGGA TGAGTATCTC AATCCGAAGC AAAAACAAGG TGAGCCAGCA TCACTCAGAT
    ACAAATTTGG GGGCCAAGCT AGCCCAGAAA ACTAGCCCCA ACCAGCCAAT CATGCCCATG
    GTTACTCAGT CTGACTTACG TTCTGTTCGC CTGAGGTCAG TCAGCAAGTC TGAGCTGGAA
    GATGACACTG AGAGCCCTGA CTACGCTGAG GAATCAGGAG CTGAAGTCTT CACCTTGCCA
    GAGAGGAAGA TGAAACCTCC CATAGCCGAG AAGCCCCCTC TGGCCCGAAG GCCTCCAAGC
    TTAGTCCACA AGCCACCATC TGTCCCCGAG GAGTACCCAC TAACTTCGCC TACATTGGCT
    ATGACTCCCA AGAGCTCAAT TCAGCACATA AGGCCACTCC CTCAAGATAT CTACACAGTG
    GTGCGGAAAT CAAAGTCCTC CAGCTTCCCT GAGAGCAGAA GCCCAGGGGA GTCCACAGCA
    CCCTCATCTC TTGTTTTCAC GCCTTTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGGAAG TATGGAGGAT GAGGGCCCTA AGGGGAGAGC CCTGCCTGAA
    AGAATTAGCC TCCAGAGCCA AGAAGAAGCT GAGAAAAAGA AAGGCAAGAT TCCACCTCCT
    GTACCAAAAA AGCCCAGCGT GCTGTACCTG CCTCTCACTT CACCCATGGC TCAAGCGGAG
    GCCTATGTGA CTGAACCAAG ACTGCCCCTC AGCCCCATCA TCACCCTGGA GGAAGATGCC
    AAGTGTCTCC CAACTGATGA CCACCTGCCA TCTGCTGGTA CAAGGATGAC CTCAAGGCCA
    CAGTCCAACA GCGAAAGGGA AGCAAGTCCT CCAGGGAGTT TGATGGAACC AAGCACCGAA
    GAAAAAAGTG TAATCAGTGA TAAAACAGCC GAATGGATTG CAGAAGATGA TGATGATGTG
    TTTGTGGCTT CACGTACAAC TGAAGATTTA TTTACTGTGA TCCACAGGTC CAAAAGAAAG
    CTGCTTGGCT GGAAGGAACC TGGTGAGACC TTTGCTGGCA GCAGCAGACC AAGCTCCCAC
    TCACCAGTAA GGAACCCACC TGAGTCTCCG GTCAGTGAGT TGGCTGCCTC TTCAGGGTCA
    AGCGGCAGTG CCAACCTAGA TGCTGGCAGA AATGATGATT TCAAGGCCTT GCTACAGAAG
    AAGGGAAGCA AGGCAACTCC AAGGTCCCGC CCCTCAGCAG CCGAACTGCT GAAGACCACT
    AACCCACTGG CTCGGCGAAT TATTGCACAA TTTTCAAAAG ACTACAAAAC CCCTGATAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89355
    Clone ID Related Accession (Same CDS sequence) XM_015461643.2 , XM_015470421.1 , XM_015461643.1
    Accession Version XM_015461643.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA
    GGCAGTGTGG CCCGCTCTAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT
    GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA
    CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC
    CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA
    GAATCGACAT TCTCTGCTTC CAGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC
    ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAGTC CTTCCTCTGG GAATTCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT
    CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TGCTCAACGA
    AGCCAAGTAT CTGAACGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG
    GAGACAGGCC CAGGTGGGGC CAGCAAGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT
    ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA
    CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT
    GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC
    AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC
    TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC
    TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA
    ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACTGGCAC CACAGTCATT
    GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC
    ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG
    CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG
    CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAGAAAATG
    TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT
    GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGATACAAAT
    TTGGGGGCCA AGCTAGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC CATGGTTACT
    CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC
    ACTGAGAGCC CTGACTACGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTCC AAGCTTAGTC
    CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCACTAACTT CGCCTACATT GGCTATGACT
    CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG
    AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA
    TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT
    AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA
    AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA TGGCTCAAGC GGAGGCCTAT
    GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA TGCCAAGTGT
    CTCCCAACTG ATGACCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC
    AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA
    AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG
    GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA
    GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTTCAGG GTCAAGCGGC
    AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA
    AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA
    CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317129.1
    CDS75..3500
    Translation

    Target ORF information:

    RefSeq Version XM_015461643.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461643.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA
    GGCAGTGTGG CCCGCTCTAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT
    GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA
    CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC
    CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA
    GAATCGACAT TCTCTGCTTC CAGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC
    ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAGTC CTTCCTCTGG GAATTCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT
    CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TGCTCAACGA
    AGCCAAGTAT CTGAACGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG
    GAGACAGGCC CAGGTGGGGC CAGCAAGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT
    ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA
    CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT
    GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC
    AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC
    TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC
    TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA
    ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACTGGCAC CACAGTCATT
    GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC
    ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG
    CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG
    CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAGAAAATG
    TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT
    GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGATACAAAT
    TTGGGGGCCA AGCTAGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC CATGGTTACT
    CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC
    ACTGAGAGCC CTGACTACGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTCC AAGCTTAGTC
    CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCACTAACTT CGCCTACATT GGCTATGACT
    CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG
    AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA
    TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT
    AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA
    AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA TGGCTCAAGC GGAGGCCTAT
    GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA TGCCAAGTGT
    CTCCCAACTG ATGACCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC
    AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA
    AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG
    GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA
    GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTTCAGG GTCAAGCGGC
    AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA
    AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA
    CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89355
    Clone ID Related Accession (Same CDS sequence) XM_015461643.2 , XM_015470421.1 , XM_015461643.1
    Accession Version XM_015470421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA
    GGCAGTGTGG CCCGCTCTAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT
    GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA
    CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC
    CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA
    GAATCGACAT TCTCTGCTTC CAGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC
    ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAGTC CTTCCTCTGG GAATTCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT
    CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TGCTCAACGA
    AGCCAAGTAT CTGAACGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG
    GAGACAGGCC CAGGTGGGGC CAGCAAGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT
    ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA
    CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT
    GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC
    AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC
    TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC
    TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA
    ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACTGGCAC CACAGTCATT
    GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC
    ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG
    CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG
    CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAGAAAATG
    TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT
    GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGATACAAAT
    TTGGGGGCCA AGCTAGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC CATGGTTACT
    CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC
    ACTGAGAGCC CTGACTACGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTCC AAGCTTAGTC
    CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCACTAACTT CGCCTACATT GGCTATGACT
    CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG
    AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA
    TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT
    AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA
    AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA TGGCTCAAGC GGAGGCCTAT
    GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA TGCCAAGTGT
    CTCCCAACTG ATGACCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC
    AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA
    AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG
    GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA
    GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTTCAGG GTCAAGCGGC
    AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA
    AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA
    CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325907.1
    CDS863..4288
    Translation

    Target ORF information:

    RefSeq Version XM_015470421.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA
    GGCAGTGTGG CCCGCTCTAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT
    GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA
    CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC
    CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA
    GAATCGACAT TCTCTGCTTC CAGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC
    ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAGTC CTTCCTCTGG GAATTCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT
    CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TGCTCAACGA
    AGCCAAGTAT CTGAACGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG
    GAGACAGGCC CAGGTGGGGC CAGCAAGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT
    ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA
    CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT
    GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC
    AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC
    TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC
    TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA
    ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACTGGCAC CACAGTCATT
    GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC
    ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG
    CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG
    CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAGAAAATG
    TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT
    GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGATACAAAT
    TTGGGGGCCA AGCTAGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC CATGGTTACT
    CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC
    ACTGAGAGCC CTGACTACGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTCC AAGCTTAGTC
    CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCACTAACTT CGCCTACATT GGCTATGACT
    CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG
    AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA
    TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT
    AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA
    AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA TGGCTCAAGC GGAGGCCTAT
    GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA TGCCAAGTGT
    CTCCCAACTG ATGACCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC
    AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA
    AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG
    GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA
    GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTTCAGG GTCAAGCGGC
    AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA
    AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA
    CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89355
    Clone ID Related Accession (Same CDS sequence) XM_015461643.2 , XM_015470421.1 , XM_015461643.1
    Accession Version XM_015461643.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA
    GGCAGTGTGG CCCGCTCTAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT
    GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA
    CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC
    CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA
    GAATCGACAT TCTCTGCTTC CAGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC
    ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAGTC CTTCCTCTGG GAATTCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT
    CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TGCTCAACGA
    AGCCAAGTAT CTGAACGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG
    GAGACAGGCC CAGGTGGGGC CAGCAAGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT
    ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA
    CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT
    GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC
    AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC
    TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC
    TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA
    ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACTGGCAC CACAGTCATT
    GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC
    ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG
    CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG
    CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAGAAAATG
    TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT
    GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGATACAAAT
    TTGGGGGCCA AGCTAGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC CATGGTTACT
    CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC
    ACTGAGAGCC CTGACTACGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTCC AAGCTTAGTC
    CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCACTAACTT CGCCTACATT GGCTATGACT
    CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG
    AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA
    TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT
    AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA
    AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA TGGCTCAAGC GGAGGCCTAT
    GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA TGCCAAGTGT
    CTCCCAACTG ATGACCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC
    AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA
    AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG
    GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA
    GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTTCAGG GTCAAGCGGC
    AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA
    AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA
    CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317129.1
    CDS863..4288
    Translation

    Target ORF information:

    RefSeq Version XM_015461643.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG
    TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG
    AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG TCCCACACAC
    TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA
    GGCAGTGTGG CCCGCTCTAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT
    GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA
    CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC
    CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA
    GAATCGACAT TCTCTGCTTC CAGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC
    ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAGTC CTTCCTCTGG GAATTCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT
    CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TGCTCAACGA
    AGCCAAGTAT CTGAACGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG
    GAGACAGGCC CAGGTGGGGC CAGCAAGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT
    ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA
    CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT
    GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC
    AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC
    TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC
    TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA
    ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACTGGCAC CACAGTCATT
    GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC
    ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG
    CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG
    CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAGAAAATG
    TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT
    GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGATACAAAT
    TTGGGGGCCA AGCTAGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC CATGGTTACT
    CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC
    ACTGAGAGCC CTGACTACGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTCC AAGCTTAGTC
    CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCACTAACTT CGCCTACATT GGCTATGACT
    CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG
    AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA
    TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT
    AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA
    AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA TGGCTCAAGC GGAGGCCTAT
    GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA TGCCAAGTGT
    CTCCCAACTG ATGACCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC
    AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA
    AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG
    GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA
    GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTTCAGG GTCAAGCGGC
    AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA
    AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA
    CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89356
    Clone ID Related Accession (Same CDS sequence) XM_015461644.1 , XM_015470422.1
    Accession Version XM_015461644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGAAA CCCGCCACAG 
    CCGCTGACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCGTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GTTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAG TCTGTTCCGG CGCACCGTGC GCCTCCGCCG CCGCCTTCCC
    TACCGCCTGC TCGGCCGGGA GGACCACGAG GAAGAGTTAG GTGCCTCCCA TTGGTCAAAC
    CTAACCCGAA GCCAGAGGGC GAGGGAGCCT GTCAATGCAG TCCATACAGG TCACAGCAAC
    AGCCCAGCAG GCAGTGTGGC CCGCTCTACC ACCTCAGACA TCAGGCCCAG CCATTCAGTT
    CCAGAAAGTG TTCATGGAAG AGTTGCACTT GGTCAGGAAG CTCGGTTCCC AAATCTCACC
    TCACCAGTAC TAAGAAATCC TTCTAGTCAT CCGGAAGAAC CTCCCCAGGC ATGGGGTGCC
    ACAAAACCCC CTGGAATGGA GAGCATGGGG ATGGTGTACA GCATTCCTGG TTCTTGCAAT
    GGACCAACAG AATCGACATT CTCTGCTTCC AGGAAGGGAG ATGCTTTTAC CTATGTGACT
    CCAAGTGCCA CCGCTCAGAG CAGTCAAGTC AATGAAAATG GAAAAAGTCC TTCCTCTGGG
    AATTCTTGGG TCTCTCTGCA CACACTGCCA CCTCTTGTTC CTAAGGAGGC TGCTACCCTC
    TTTGTCACTC GTGATATCCC AGCAGGGTGC AGTGGGCCTG CTGGCTACTC TCAGCATCCT
    GCTCAACGAA GCCAAGTATC TGAACGACCC TCCAAGATTG GCCTTCTGAC CGGTGGTACC
    TCAAGGCTGG AGACAGGCCC AGGTGGGGCC AGCAAGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGATA CAGGTACCAT GGATGTGGAC TATGAGGAGG AGCAGAAGGC CAGTGAAGCC
    TGTGCCCTAC CTTATGCCAG TACGAGTTCT GAGGGCAGTA ACAGTGCTGA CAACATTGCC
    TCCCTCAGTG CCCAACAGGA GGCCCATCAC AGAAGGCAGA GGTCCAAGAG CATCTCACTC
    AAGAAGGCCA AGAAGAAGCC TTCCCCTCCG ACTCGCAGTG TCTCACTGGT CAAAGATGAA
    CCAGTCCTCT TGCCGGAAGG TGGGTCAGTA CTACCCAAGG ACCAGAGGCC CAGGAGCCTT
    TGTCTCTCCT TGGAACACCA AGGACATCAC TCATCCCACC CAGATACTCA GGGTCACCCA
    GCTGTGCCAA CCTTCAAAGA CCCAGAAGGT ACACAATTCG CCCATCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACTGGCACC
    ACAGTCATTG AGTGCACCCA AGTTCAGGGC AGCTCAGAGT CTCTTGCCTC CCCTTCCACT
    TCCAGAGCCA CGACGCCTTC CCAGCTTTCC ATCGAGGTGG AGGCCAGGGA GGTATCCTCT
    CCAGGAAGGC CTACTGGGCT GATGTCACCC TCCAGTGGAT ACTCCAGCCA GTCAGAGACG
    CCAACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TGCCCCCTCC AAGCAGCAGT
    GTCCGGGTGC GTCCAGTGGT ACCTGAGAGG AAGTCATCAC TACCCCCAAC ATCACCAATG
    GAGAAAATGT CCAAGTCACG GCTATCGTTT GACCTACCAT TGACCTCTTC AACCAACCTG
    GATCTGTCTG GGATGAGTAT CTCAATCCGA AGCAAAAACA AGGTGAGCCA GCATCACTCA
    GATACAAATT TGGGGGCCAA GCTAGCCCAG AAAACTAGCC CCAACCAGCC AATCATGCCC
    ATGGTTACTC AGTCTGACTT ACGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCTG
    GAAGATGACA CTGAGAGCCC TGACTACGCT GAGGAATCAG GAGCTGAAGT CTTCACCTTG
    CCAGAGAGGA AGATGAAACC TCCCATAGCC GAGAAGCCCC CTCTGGCCCG AAGGCCTCCA
    AGCTTAGTCC ACAAGCCACC ATCTGTCCCC GAGGAGTACC CACTAACTTC GCCTACATTG
    GCTATGACTC CCAAGAGCTC AATTCAGCAC ATAAGGCCAC TCCCTCAAGA TATCTACACA
    GTGGTGCGGA AATCAAAGTC CTCCAGCTTC CCTGAGAGCA GAAGCCCAGG GGAGTCCACA
    GCACCCTCAT CTCTTGTTTT CACGCCTTTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGG AAGTATGGAG GATGAGGGCC CTAAGGGGAG AGCCCTGCCT
    GAAAGAATTA GCCTCCAGAG CCAAGAAGAA GCTGAGAAAA AGAAAGGCAA GATTCCACCT
    CCTGTACCAA AAAAGCCCAG CGTGCTGTAC CTGCCTCTCA CTTCACCCAT GGCTCAAGCG
    GAGGCCTATG TGACTGAACC AAGACTGCCC CTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GCCAAGTGTC TCCCAACTGA TGACCACCTG CCATCTGCTG GTACAAGGAT GACCTCAAGG
    CCACAGTCCA ACAGCGAAAG GGAAGCAAGT CCTCCAGGGA GTTTGATGGA ACCAAGCACC
    GAAGAAAAAA GTGTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAAGA TGATGATGAT
    GTGTTTGTGG CTTCACGTAC AACTGAAGAT TTATTTACTG TGATCCACAG GTCCAAAAGA
    AAGCTGCTTG GCTGGAAGGA ACCTGGTGAG ACCTTTGCTG GCAGCAGCAG ACCAAGCTCC
    CACTCACCAG TAAGGAACCC ACCTGAGTCT CCGGTCAGTG AGTTGGCTGC CTCTTCAGGG
    TCAAGCGGCA GTGCCAACCT AGATGCTGGC AGAAATGATG ATTTCAAGGC CTTGCTACAG
    AAGAAGGGAA GCAAGGCAAC TCCAAGGTCC CGCCCCTCAG CAGCCGAACT GCTGAAGACC
    ACTAACCCAC TGGCTCGGCG AATTATTGCA CAATTTTCAA AAGACTACAA AACCCCTGAT
    AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317130.1
    CDS38..3172
    Translation

    Target ORF information:

    RefSeq Version XM_015461644.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGAAA CCCGCCACAG 
    CCGCTGACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCGTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GTTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAG TCTGTTCCGG CGCACCGTGC GCCTCCGCCG CCGCCTTCCC
    TACCGCCTGC TCGGCCGGGA GGACCACGAG GAAGAGTTAG GTGCCTCCCA TTGGTCAAAC
    CTAACCCGAA GCCAGAGGGC GAGGGAGCCT GTCAATGCAG TCCATACAGG TCACAGCAAC
    AGCCCAGCAG GCAGTGTGGC CCGCTCTACC ACCTCAGACA TCAGGCCCAG CCATTCAGTT
    CCAGAAAGTG TTCATGGAAG AGTTGCACTT GGTCAGGAAG CTCGGTTCCC AAATCTCACC
    TCACCAGTAC TAAGAAATCC TTCTAGTCAT CCGGAAGAAC CTCCCCAGGC ATGGGGTGCC
    ACAAAACCCC CTGGAATGGA GAGCATGGGG ATGGTGTACA GCATTCCTGG TTCTTGCAAT
    GGACCAACAG AATCGACATT CTCTGCTTCC AGGAAGGGAG ATGCTTTTAC CTATGTGACT
    CCAAGTGCCA CCGCTCAGAG CAGTCAAGTC AATGAAAATG GAAAAAGTCC TTCCTCTGGG
    AATTCTTGGG TCTCTCTGCA CACACTGCCA CCTCTTGTTC CTAAGGAGGC TGCTACCCTC
    TTTGTCACTC GTGATATCCC AGCAGGGTGC AGTGGGCCTG CTGGCTACTC TCAGCATCCT
    GCTCAACGAA GCCAAGTATC TGAACGACCC TCCAAGATTG GCCTTCTGAC CGGTGGTACC
    TCAAGGCTGG AGACAGGCCC AGGTGGGGCC AGCAAGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGATA CAGGTACCAT GGATGTGGAC TATGAGGAGG AGCAGAAGGC CAGTGAAGCC
    TGTGCCCTAC CTTATGCCAG TACGAGTTCT GAGGGCAGTA ACAGTGCTGA CAACATTGCC
    TCCCTCAGTG CCCAACAGGA GGCCCATCAC AGAAGGCAGA GGTCCAAGAG CATCTCACTC
    AAGAAGGCCA AGAAGAAGCC TTCCCCTCCG ACTCGCAGTG TCTCACTGGT CAAAGATGAA
    CCAGTCCTCT TGCCGGAAGG TGGGTCAGTA CTACCCAAGG ACCAGAGGCC CAGGAGCCTT
    TGTCTCTCCT TGGAACACCA AGGACATCAC TCATCCCACC CAGATACTCA GGGTCACCCA
    GCTGTGCCAA CCTTCAAAGA CCCAGAAGGT ACACAATTCG CCCATCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACTGGCACC
    ACAGTCATTG AGTGCACCCA AGTTCAGGGC AGCTCAGAGT CTCTTGCCTC CCCTTCCACT
    TCCAGAGCCA CGACGCCTTC CCAGCTTTCC ATCGAGGTGG AGGCCAGGGA GGTATCCTCT
    CCAGGAAGGC CTACTGGGCT GATGTCACCC TCCAGTGGAT ACTCCAGCCA GTCAGAGACG
    CCAACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TGCCCCCTCC AAGCAGCAGT
    GTCCGGGTGC GTCCAGTGGT ACCTGAGAGG AAGTCATCAC TACCCCCAAC ATCACCAATG
    GAGAAAATGT CCAAGTCACG GCTATCGTTT GACCTACCAT TGACCTCTTC AACCAACCTG
    GATCTGTCTG GGATGAGTAT CTCAATCCGA AGCAAAAACA AGGTGAGCCA GCATCACTCA
    GATACAAATT TGGGGGCCAA GCTAGCCCAG AAAACTAGCC CCAACCAGCC AATCATGCCC
    ATGGTTACTC AGTCTGACTT ACGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCTG
    GAAGATGACA CTGAGAGCCC TGACTACGCT GAGGAATCAG GAGCTGAAGT CTTCACCTTG
    CCAGAGAGGA AGATGAAACC TCCCATAGCC GAGAAGCCCC CTCTGGCCCG AAGGCCTCCA
    AGCTTAGTCC ACAAGCCACC ATCTGTCCCC GAGGAGTACC CACTAACTTC GCCTACATTG
    GCTATGACTC CCAAGAGCTC AATTCAGCAC ATAAGGCCAC TCCCTCAAGA TATCTACACA
    GTGGTGCGGA AATCAAAGTC CTCCAGCTTC CCTGAGAGCA GAAGCCCAGG GGAGTCCACA
    GCACCCTCAT CTCTTGTTTT CACGCCTTTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGG AAGTATGGAG GATGAGGGCC CTAAGGGGAG AGCCCTGCCT
    GAAAGAATTA GCCTCCAGAG CCAAGAAGAA GCTGAGAAAA AGAAAGGCAA GATTCCACCT
    CCTGTACCAA AAAAGCCCAG CGTGCTGTAC CTGCCTCTCA CTTCACCCAT GGCTCAAGCG
    GAGGCCTATG TGACTGAACC AAGACTGCCC CTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GCCAAGTGTC TCCCAACTGA TGACCACCTG CCATCTGCTG GTACAAGGAT GACCTCAAGG
    CCACAGTCCA ACAGCGAAAG GGAAGCAAGT CCTCCAGGGA GTTTGATGGA ACCAAGCACC
    GAAGAAAAAA GTGTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAAGA TGATGATGAT
    GTGTTTGTGG CTTCACGTAC AACTGAAGAT TTATTTACTG TGATCCACAG GTCCAAAAGA
    AAGCTGCTTG GCTGGAAGGA ACCTGGTGAG ACCTTTGCTG GCAGCAGCAG ACCAAGCTCC
    CACTCACCAG TAAGGAACCC ACCTGAGTCT CCGGTCAGTG AGTTGGCTGC CTCTTCAGGG
    TCAAGCGGCA GTGCCAACCT AGATGCTGGC AGAAATGATG ATTTCAAGGC CTTGCTACAG
    AAGAAGGGAA GCAAGGCAAC TCCAAGGTCC CGCCCCTCAG CAGCCGAACT GCTGAAGACC
    ACTAACCCAC TGGCTCGGCG AATTATTGCA CAATTTTCAA AAGACTACAA AACCCCTGAT
    AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89356
    Clone ID Related Accession (Same CDS sequence) XM_015461644.1 , XM_015470422.1
    Accession Version XM_015470422.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NHS-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGAAA CCCGCCACAG 
    CCGCTGACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCGTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GTTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAG TCTGTTCCGG CGCACCGTGC GCCTCCGCCG CCGCCTTCCC
    TACCGCCTGC TCGGCCGGGA GGACCACGAG GAAGAGTTAG GTGCCTCCCA TTGGTCAAAC
    CTAACCCGAA GCCAGAGGGC GAGGGAGCCT GTCAATGCAG TCCATACAGG TCACAGCAAC
    AGCCCAGCAG GCAGTGTGGC CCGCTCTACC ACCTCAGACA TCAGGCCCAG CCATTCAGTT
    CCAGAAAGTG TTCATGGAAG AGTTGCACTT GGTCAGGAAG CTCGGTTCCC AAATCTCACC
    TCACCAGTAC TAAGAAATCC TTCTAGTCAT CCGGAAGAAC CTCCCCAGGC ATGGGGTGCC
    ACAAAACCCC CTGGAATGGA GAGCATGGGG ATGGTGTACA GCATTCCTGG TTCTTGCAAT
    GGACCAACAG AATCGACATT CTCTGCTTCC AGGAAGGGAG ATGCTTTTAC CTATGTGACT
    CCAAGTGCCA CCGCTCAGAG CAGTCAAGTC AATGAAAATG GAAAAAGTCC TTCCTCTGGG
    AATTCTTGGG TCTCTCTGCA CACACTGCCA CCTCTTGTTC CTAAGGAGGC TGCTACCCTC
    TTTGTCACTC GTGATATCCC AGCAGGGTGC AGTGGGCCTG CTGGCTACTC TCAGCATCCT
    GCTCAACGAA GCCAAGTATC TGAACGACCC TCCAAGATTG GCCTTCTGAC CGGTGGTACC
    TCAAGGCTGG AGACAGGCCC AGGTGGGGCC AGCAAGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGATA CAGGTACCAT GGATGTGGAC TATGAGGAGG AGCAGAAGGC CAGTGAAGCC
    TGTGCCCTAC CTTATGCCAG TACGAGTTCT GAGGGCAGTA ACAGTGCTGA CAACATTGCC
    TCCCTCAGTG CCCAACAGGA GGCCCATCAC AGAAGGCAGA GGTCCAAGAG CATCTCACTC
    AAGAAGGCCA AGAAGAAGCC TTCCCCTCCG ACTCGCAGTG TCTCACTGGT CAAAGATGAA
    CCAGTCCTCT TGCCGGAAGG TGGGTCAGTA CTACCCAAGG ACCAGAGGCC CAGGAGCCTT
    TGTCTCTCCT TGGAACACCA AGGACATCAC TCATCCCACC CAGATACTCA GGGTCACCCA
    GCTGTGCCAA CCTTCAAAGA CCCAGAAGGT ACACAATTCG CCCATCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACTGGCACC
    ACAGTCATTG AGTGCACCCA AGTTCAGGGC AGCTCAGAGT CTCTTGCCTC CCCTTCCACT
    TCCAGAGCCA CGACGCCTTC CCAGCTTTCC ATCGAGGTGG AGGCCAGGGA GGTATCCTCT
    CCAGGAAGGC CTACTGGGCT GATGTCACCC TCCAGTGGAT ACTCCAGCCA GTCAGAGACG
    CCAACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TGCCCCCTCC AAGCAGCAGT
    GTCCGGGTGC GTCCAGTGGT ACCTGAGAGG AAGTCATCAC TACCCCCAAC ATCACCAATG
    GAGAAAATGT CCAAGTCACG GCTATCGTTT GACCTACCAT TGACCTCTTC AACCAACCTG
    GATCTGTCTG GGATGAGTAT CTCAATCCGA AGCAAAAACA AGGTGAGCCA GCATCACTCA
    GATACAAATT TGGGGGCCAA GCTAGCCCAG AAAACTAGCC CCAACCAGCC AATCATGCCC
    ATGGTTACTC AGTCTGACTT ACGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCTG
    GAAGATGACA CTGAGAGCCC TGACTACGCT GAGGAATCAG GAGCTGAAGT CTTCACCTTG
    CCAGAGAGGA AGATGAAACC TCCCATAGCC GAGAAGCCCC CTCTGGCCCG AAGGCCTCCA
    AGCTTAGTCC ACAAGCCACC ATCTGTCCCC GAGGAGTACC CACTAACTTC GCCTACATTG
    GCTATGACTC CCAAGAGCTC AATTCAGCAC ATAAGGCCAC TCCCTCAAGA TATCTACACA
    GTGGTGCGGA AATCAAAGTC CTCCAGCTTC CCTGAGAGCA GAAGCCCAGG GGAGTCCACA
    GCACCCTCAT CTCTTGTTTT CACGCCTTTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGG AAGTATGGAG GATGAGGGCC CTAAGGGGAG AGCCCTGCCT
    GAAAGAATTA GCCTCCAGAG CCAAGAAGAA GCTGAGAAAA AGAAAGGCAA GATTCCACCT
    CCTGTACCAA AAAAGCCCAG CGTGCTGTAC CTGCCTCTCA CTTCACCCAT GGCTCAAGCG
    GAGGCCTATG TGACTGAACC AAGACTGCCC CTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GCCAAGTGTC TCCCAACTGA TGACCACCTG CCATCTGCTG GTACAAGGAT GACCTCAAGG
    CCACAGTCCA ACAGCGAAAG GGAAGCAAGT CCTCCAGGGA GTTTGATGGA ACCAAGCACC
    GAAGAAAAAA GTGTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAAGA TGATGATGAT
    GTGTTTGTGG CTTCACGTAC AACTGAAGAT TTATTTACTG TGATCCACAG GTCCAAAAGA
    AAGCTGCTTG GCTGGAAGGA ACCTGGTGAG ACCTTTGCTG GCAGCAGCAG ACCAAGCTCC
    CACTCACCAG TAAGGAACCC ACCTGAGTCT CCGGTCAGTG AGTTGGCTGC CTCTTCAGGG
    TCAAGCGGCA GTGCCAACCT AGATGCTGGC AGAAATGATG ATTTCAAGGC CTTGCTACAG
    AAGAAGGGAA GCAAGGCAAC TCCAAGGTCC CGCCCCTCAG CAGCCGAACT GCTGAAGACC
    ACTAACCCAC TGGCTCGGCG AATTATTGCA CAATTTTCAA AAGACTACAA AACCCCTGAT
    AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325908.1
    CDS38..3172
    Translation

    Target ORF information:

    RefSeq Version XM_015470422.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NHS like 2 (NHSL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470422.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGAAA CCCGCCACAG 
    CCGCTGACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCGTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GTTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAG TCTGTTCCGG CGCACCGTGC GCCTCCGCCG CCGCCTTCCC
    TACCGCCTGC TCGGCCGGGA GGACCACGAG GAAGAGTTAG GTGCCTCCCA TTGGTCAAAC
    CTAACCCGAA GCCAGAGGGC GAGGGAGCCT GTCAATGCAG TCCATACAGG TCACAGCAAC
    AGCCCAGCAG GCAGTGTGGC CCGCTCTACC ACCTCAGACA TCAGGCCCAG CCATTCAGTT
    CCAGAAAGTG TTCATGGAAG AGTTGCACTT GGTCAGGAAG CTCGGTTCCC AAATCTCACC
    TCACCAGTAC TAAGAAATCC TTCTAGTCAT CCGGAAGAAC CTCCCCAGGC ATGGGGTGCC
    ACAAAACCCC CTGGAATGGA GAGCATGGGG ATGGTGTACA GCATTCCTGG TTCTTGCAAT
    GGACCAACAG AATCGACATT CTCTGCTTCC AGGAAGGGAG ATGCTTTTAC CTATGTGACT
    CCAAGTGCCA CCGCTCAGAG CAGTCAAGTC AATGAAAATG GAAAAAGTCC TTCCTCTGGG
    AATTCTTGGG TCTCTCTGCA CACACTGCCA CCTCTTGTTC CTAAGGAGGC TGCTACCCTC
    TTTGTCACTC GTGATATCCC AGCAGGGTGC AGTGGGCCTG CTGGCTACTC TCAGCATCCT
    GCTCAACGAA GCCAAGTATC TGAACGACCC TCCAAGATTG GCCTTCTGAC CGGTGGTACC
    TCAAGGCTGG AGACAGGCCC AGGTGGGGCC AGCAAGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGATA CAGGTACCAT GGATGTGGAC TATGAGGAGG AGCAGAAGGC CAGTGAAGCC
    TGTGCCCTAC CTTATGCCAG TACGAGTTCT GAGGGCAGTA ACAGTGCTGA CAACATTGCC
    TCCCTCAGTG CCCAACAGGA GGCCCATCAC AGAAGGCAGA GGTCCAAGAG CATCTCACTC
    AAGAAGGCCA AGAAGAAGCC TTCCCCTCCG ACTCGCAGTG TCTCACTGGT CAAAGATGAA
    CCAGTCCTCT TGCCGGAAGG TGGGTCAGTA CTACCCAAGG ACCAGAGGCC CAGGAGCCTT
    TGTCTCTCCT TGGAACACCA AGGACATCAC TCATCCCACC CAGATACTCA GGGTCACCCA
    GCTGTGCCAA CCTTCAAAGA CCCAGAAGGT ACACAATTCG CCCATCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACTGGCACC
    ACAGTCATTG AGTGCACCCA AGTTCAGGGC AGCTCAGAGT CTCTTGCCTC CCCTTCCACT
    TCCAGAGCCA CGACGCCTTC CCAGCTTTCC ATCGAGGTGG AGGCCAGGGA GGTATCCTCT
    CCAGGAAGGC CTACTGGGCT GATGTCACCC TCCAGTGGAT ACTCCAGCCA GTCAGAGACG
    CCAACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TGCCCCCTCC AAGCAGCAGT
    GTCCGGGTGC GTCCAGTGGT ACCTGAGAGG AAGTCATCAC TACCCCCAAC ATCACCAATG
    GAGAAAATGT CCAAGTCACG GCTATCGTTT GACCTACCAT TGACCTCTTC AACCAACCTG
    GATCTGTCTG GGATGAGTAT CTCAATCCGA AGCAAAAACA AGGTGAGCCA GCATCACTCA
    GATACAAATT TGGGGGCCAA GCTAGCCCAG AAAACTAGCC CCAACCAGCC AATCATGCCC
    ATGGTTACTC AGTCTGACTT ACGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCTG
    GAAGATGACA CTGAGAGCCC TGACTACGCT GAGGAATCAG GAGCTGAAGT CTTCACCTTG
    CCAGAGAGGA AGATGAAACC TCCCATAGCC GAGAAGCCCC CTCTGGCCCG AAGGCCTCCA
    AGCTTAGTCC ACAAGCCACC ATCTGTCCCC GAGGAGTACC CACTAACTTC GCCTACATTG
    GCTATGACTC CCAAGAGCTC AATTCAGCAC ATAAGGCCAC TCCCTCAAGA TATCTACACA
    GTGGTGCGGA AATCAAAGTC CTCCAGCTTC CCTGAGAGCA GAAGCCCAGG GGAGTCCACA
    GCACCCTCAT CTCTTGTTTT CACGCCTTTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGG AAGTATGGAG GATGAGGGCC CTAAGGGGAG AGCCCTGCCT
    GAAAGAATTA GCCTCCAGAG CCAAGAAGAA GCTGAGAAAA AGAAAGGCAA GATTCCACCT
    CCTGTACCAA AAAAGCCCAG CGTGCTGTAC CTGCCTCTCA CTTCACCCAT GGCTCAAGCG
    GAGGCCTATG TGACTGAACC AAGACTGCCC CTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GCCAAGTGTC TCCCAACTGA TGACCACCTG CCATCTGCTG GTACAAGGAT GACCTCAAGG
    CCACAGTCCA ACAGCGAAAG GGAAGCAAGT CCTCCAGGGA GTTTGATGGA ACCAAGCACC
    GAAGAAAAAA GTGTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAAGA TGATGATGAT
    GTGTTTGTGG CTTCACGTAC AACTGAAGAT TTATTTACTG TGATCCACAG GTCCAAAAGA
    AAGCTGCTTG GCTGGAAGGA ACCTGGTGAG ACCTTTGCTG GCAGCAGCAG ACCAAGCTCC
    CACTCACCAG TAAGGAACCC ACCTGAGTCT CCGGTCAGTG AGTTGGCTGC CTCTTCAGGG
    TCAAGCGGCA GTGCCAACCT AGATGCTGGC AGAAATGATG ATTTCAAGGC CTTGCTACAG
    AAGAAGGGAA GCAAGGCAAC TCCAAGGTCC CGCCCCTCAG CAGCCGAACT GCTGAAGACC
    ACTAACCCAC TGGCTCGGCG AATTATTGCA CAATTTTCAA AAGACTACAA AACCCCTGAT
    AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.