nhsl2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nhsl2
Entrez Gene ID	100486042
Full Name	NHS like 2
General protein information	Preferred Names NHS like 2 Names NHS-like protein 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004916790.3	XP_004916847.1	NHS-like protein 2 isoform X1
XM_012968902.2	XP_012824356.1	NHS-like protein 2 isoform X2
XM_012968902.3	XP_012824356.2	NHS-like protein 2 isoform X2
XM_031891006.1	XP_031746866.1	NHS-like protein 2 isoform X5
XM_031891005.1	XP_031746865.1	NHS-like protein 2 isoform X4
XM_004916790.4	XP_004916847.2	NHS-like protein 2 isoform X1
XM_031891004.1	XP_031746864.1	NHS-like protein 2 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	NHSL2	NP_001013649.2
Rattus norvegicus (Norway rat)	Nhsl2	XP_006257183.1
Gallus gallus (chicken)	NHSL2	XP_420135.4
Canis lupus familiaris (dog)	NHSL2	XP_549074.3
Mus musculus (house mouse)	Nhsl2	NP_001157082.1
Danio rerio (zebrafish)	LOC794153	XP_001334053.3
Xenopus tropicalis (tropical clawed frog)	nhsl2	XP_004916848.1
Pan troglodytes (chimpanzee)	NHSL2	XP_001139391.2
Bos taurus (cattle)	NHSL2	XP_002700058.1
	LOC702808	XP_002806324.1

Gene Ontology

The following nhsl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nhsl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa14263	XM_031891004.1 Latest version!	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa14264	XM_031891005.1 Latest version!	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa14265	XM_031891006.1 Latest version!	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa64261	XM_004916790.4 Latest version!	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa64262	XM_012968902.3 Latest version!	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa14261	XM_004916790.3 Latest version!	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa33512	XM_012968902.2 Latest version!	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nhsl2 ( XM_031891004.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746864.1 Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa14263
Clone ID Related Accession (Same CDS sequence)	XM_031891004.1
Accession Version	XM_031891004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGGA ACTCACCGCA CAAACAGAGA AGCAAGAAGC ATCACAGAAT GCAGCCGGCA 
ACAGCTGCTG CTCACTGCCC GACCACCACA GCTGCTTCTG CTGTGGACCA ATCGAAGTTG 
GCAAACTGGC AACAACAGCC AGTAAATGTT TTTCTGTCCA CTAAGCGGCC TCCTTGCGTG 
GAAGAGCTTC ATCAAGAGGC TGAACTGAAC CTGCAGAGCC TTCTTCAGGC TGATTGCTTT 
ACAGAAGAAT TTGAAGATCG CTATCTAGAC ACTAAAGTCA CTGGGCAAAC ATTCCGCTAT 
TCTGCCCTCT CGCAATCTGC AACACTTCCT CCAGAGACTC CCACCAGGAA TACCCCCAAG 
CGCCTTGAGT TTGTATATCT GCCAACCAAC AGAAGAGTTA GTGAAGATGA AACCACCACT 
GCCGGTGTTA GGCCACGGGA GACATGCCTG AGTTTACCAA CAACCCCAGA CAAGCAAGTG 
GCTTGGATTA AAGCTTTCCC ATTCCCTATC CCAGAGGAGA GGAGATGGCA TCAATCTAAC 
TCCATCCAGG CAAACGTTGT ACCTATCAAT GTGTCAGGGG AACTTTTTGA TAAACATGCA 
AACTCCCGGC ATTCCTTGTT CAATACAGAG ACCGCAGTAA ATCCGAAGAC CACTCTGAGA 
CGCAGAAGGA CTGTGATTGG ATTCTCCCAC CATCAGTCAC AGGATCACGT AGACAAAACT 
CCATTGGCAG AGGCAACCCC CAAGCCAAGC ACATCAGGCA ATGTCAACGG TGTAACGTCT 
ATTGAACCTC CGCCAGATAG AGAAGAGTCC CCACCTCCTA AGCCTGAAGC ATTTCATGGG 
AGACATCCTG GAGGAAGAGC AGCTACTCCC CATCCTCAGC AGCTTCCTTT AAGAAAGACG 
TTTAGTGACC TTGGGCATAT TTTGCAGTCG CCAAGCAACA TGGACAATGC AGGTGTTATG 
TATGCTAAAC CTCCATGCAA TGGAGCGAAA GACTCTTCTA TGTCTTTAAA CTGGCAGGAA 
AGCTCATTTA ACTACATGAG TCCATCTAGC CCAGTCAAAA GCCAGCACAG TAGTGAAACA 
ACAAAACCTT CCACCTTATC AATGGCTTCT CCTGGAATGT CTAAAGGTGC AGGATCGTAT 
AAGCACAGAA AAGAGAACAT AGTGAGGAAT AACAGTGGAA ATATGTGTTC TAGTGCTACT 
CTTCCAGCCC CCACAGTAAA TCACACAAAA ACTGAAGAGT TAAAAGGCAT CTTTCACACT 
GGTCAAACAG GGGGAATCAA CTCGTCAACA TTGCCTACAC TATCTAGTGA GTGCACTTTC 
CGCGAGAGGT CATTATCAAT GCCTACTGAC TCAGGTTCCC TATCCTCTGT AGATATTGCC 
TATACAGAGA ACAGGAGAGG AAGTGGGGAC TATGCCCTAA ATTATCCTAG TGCAAGCTCT 
GAAGAGAGCA CCAGTACTGA GAATGTATCT GTTTCAGCAG AGCAAGACAA CCAGGAAAGG 
AGGAGATCTC GGAGCATCTC TTTGAGAAAG CCAAAAAAGA AGCCATCACC TCCTGCTCGC 
AGTGTTTCGT TGATTAAAGA TGCAAGTGGC CAGAAATTAC AACCGGTAGC CACCATGCCA 
AAGGACCAAC GGCCTAAAAG TCTCTGTATT GCCTTTGATA GCCAAGGGCA CAGTTATATT 
CAAACATCCA TGCATGGTAA TGCAACAATG CCAACATCAA AAAGCTTGCA CAGTTGGAAC 
CTTAGTGACT GCAAATCCAA TGACTTGTAT AGGTCTCTGT CTGGCTCTAG CACAATGACA 
GGTACCACTG TGATAGAACG CATGAAGGCA CCAGGAAGTT CAGAATCATT AAGTTCTCTT 
TCTTATTCTA GGGCCACTAC CCCCTCCCAG GTTTCTACTG AGACAGACTT AAAAATGTCT 
CCTGGAAAAC CTCCTGGCCT AATGTCACCA TCAAGTGGTT ACTCTAGTCA GTCAGAGACC 
CCAACTGCAG TGCCAACATC ATTGATTCTT GGCCACTCTC CTTACCAAAG CAGTAAGGTG 
AGACCACTTG TTCCTGAGAG AAAGTCTTCA CTGCCAGCTG TTTCACCAAT TGACAGAAGC 
CCAAAAGTCC ACCAGTCTTT TGATTTACCA ATTACTCCAT CTACACATAC ACATATGGAC 
CTAACTACAC TGCGAAGGCC TTCAAAAGGC AAAATAAGGG CCAACCGAAA TGCATCTGAA 
TCAACCTTCG GTACTTCTAA ACTTAACCAA AAGACTAGTC CAGTGCAGCC CATAATGCCT 
ATGGTTACCC AGTCTGACTT GAGATCCATA CGTTTGCGAT CAGTCAGTAA ATCAGAGCCC 
GACGACAGTT TTGATGGCAT CGATCATATC GATGAACTTG CTGACGAAGC ATTCCCGTTG 
CCTGAAAAAA AAATAAAACC TCCAGTTGCA GAGAAACCCC CAATGTCCAG AAGACCAATA 
AACCTTTCCT ACAGAAACCC TGCAGTTACA CCGGAATCCC CTGTTCTATC TCCCAGTACT 
CCTGGAACAC CTGCCAGAGA TCAAGTATCG CCACAGGATG CCTACCTGGT GATTGGAACT 
ATTAGATACA GGAAAATGAA TGATACAGAA GCTCGCATAG AAACTCCCCC TTCCTTAGAC 
CCGTCACCCC CTTTCGATAG TTGCTTTCCT GCAACCCATT CAGTGGTTAG AAGTTTTTCG 
CAGAGCAGCA TTGAAGAAGA AGAGCTCTAT AAAGGTAAAC AACTGCCTGA AAGAATAACT 
CTACAAAGCT TGAACTATAT GGATTCAAAG AAAATCAAGG TTCCACCGCC TATTCCCAAA 
AAGCCCAGTG TTTTACATCT TCCAGTTTCC AGTCCACCTC CTCCTAACCA AATGGCATCC 
TCACCTTCCG AGCAACGTTC GTCGCCGATC ATTACTCTAG ACGCTGGCAC AAGCTCTTTT 
CCTGACATTA TTGAGTTCCA AAAATATCAC GGAGTTATCT CTGCTGCTCA GCCAGATGTA 
ATAACTATTG ATCCTCTGTC AGGAGCTTTT ACAGACTTAT CAGAGGAGAA CAAAAGTGTA 
TCAAACAAAA CAGCAGAATC AATCACAGAG GATGATGATG AAGTTTTCGT CAGTTCACGC 
ACAACGGAAG ACTTATTTAC GGTCATACAC AGGTCAAAAA GGAAGTTATT GGGATGGAAA 
GATTCTGGAG ATTCTTTTGG AAGCCGGCAA AGCTCAGTGT CACCGGTAAA GAATTCAGGT 
AGCTCCACAA GTAGTGACTA TTCAACTGGG ACCATGAGCA GCTCGCGGTC ATCCAGCAAA 
AATGAAGACT TCAAAGCCCT TCTCCAGAGA AAAGGCAGCA AGAGCAACAT TGGGTCTCGT 
CCTTCAGCTG CCGAACTTCT CAAGACAACT AATCCCTTGG CACGGAGGGT CTTGAACGAG 
TTTGCTCCAG AGATTGAGAA AAGTGGCAGC ACTAAAACCT TACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746864.1
CDS	858..4325
Translation	MMGNSPHKQRSKKHHRMQPATAAAHCPTTTAASAVDQSKLANWQQQPVNVFLSTKRPPCVEELHQEAELNLQSLLQADCFTEEFEDRYLDTKVTGQTFRYSALSQSATLPPETPTRNTPKRLEFVYLPTNRRVSEDETTTAGVRPRETCLSLPTTPDKQVAWIKAFPFPIPEERRWHQSNSIQANVVPINVSGELFDKHANSRHSLFNTETAVNPKTTLRRRRTVIGFSHHQSQDHVDKTPLAEATPKPSTSGNVNGVTSIEPPPDREESPPPKPEAFHGRHPGGRAATPHPQQLPLRKTFSDLGHILQSPSNMDNAGVMYAKPPCNGAKDSSMSLNWQESSFNYMSPSSPVKSQHSSETTKPSTLSMASPGMSKGAGSYKHRKENIVRNNSGNMCSSATLPAPTVNHTKTEELKGIFHTGQTGGINSSTLPTLSSECTFRERSLSMPTDSGSLSSVDIAYTENRRGSGDYALNYPSASSEESTSTENVSVSAEQDNQERRRSRSISLRKPKKKPSPPARSVSLIKDASGQKLQPVATMPKDQRPKSLCIAFDSQGHSYIQTSMHGNATMPTSKSLHSWNLSDCKSNDLYRSLSGSSTMTGTTVIERMKAPGSSESLSSLSYSRATTPSQVSTETDLKMSPGKPPGLMSPSSGYSSQSETPTAVPTSLILGHSPYQSSKVRPLVPERKSSLPAVSPIDRSPKVHQSFDLPITPSTHTHMDLTTLRRPSKGKIRANRNASESTFGTSKLNQKTSPVQPIMPMVTQSDLRSIRLRSVSKSEPDDSFDGIDHIDELADEAFPLPEKKIKPPVAEKPPMSRRPINLSYRNPAVTPESPVLSPSTPGTPARDQVSPQDAYLVIGTIRYRKMNDTEARIETPPSLDPSPPFDSCFPATHSVVRSFSQSSIEEEELYKGKQLPERITLQSLNYMDSKKIKVPPPIPKKPSVLHLPVSSPPPPNQMASSPSEQRSSPIITLDAGTSSFPDIIEFQKYHGVISAAQPDVITIDPLSGAFTDLSEENKSVSNKTAESITEDDDEVFVSSRTTEDLFTVIHRSKRKLLGWKDSGDSFGSRQSSVSPVKNSGSSTSSDYSTGTMSSSRSSSKNEDFKALLQRKGSKSNIGSRPSAAELLKTTNPLARRVLNEFAPEIEKSGSTKTLP

Target ORF information:

RefSeq Version	XM_031891004.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891004.1

ORF Insert Sequence:

ATGATGGGGA ACTCACCGCA CAAACAGAGA AGCAAGAAGC ATCACAGAAT GCAGCCGGCA 
ACAGCTGCTG CTCACTGCCC GACCACCACA GCTGCTTCTG CTGTGGACCA ATCGAAGTTG 
GCAAACTGGC AACAACAGCC AGTAAATGTT TTTCTGTCCA CTAAGCGGCC TCCTTGCGTG 
GAAGAGCTTC ATCAAGAGGC TGAACTGAAC CTGCAGAGCC TTCTTCAGGC TGATTGCTTT 
ACAGAAGAAT TTGAAGATCG CTATCTAGAC ACTAAAGTCA CTGGGCAAAC ATTCCGCTAT 
TCTGCCCTCT CGCAATCTGC AACACTTCCT CCAGAGACTC CCACCAGGAA TACCCCCAAG 
CGCCTTGAGT TTGTATATCT GCCAACCAAC AGAAGAGTTA GTGAAGATGA AACCACCACT 
GCCGGTGTTA GGCCACGGGA GACATGCCTG AGTTTACCAA CAACCCCAGA CAAGCAAGTG 
GCTTGGATTA AAGCTTTCCC ATTCCCTATC CCAGAGGAGA GGAGATGGCA TCAATCTAAC 
TCCATCCAGG CAAACGTTGT ACCTATCAAT GTGTCAGGGG AACTTTTTGA TAAACATGCA 
AACTCCCGGC ATTCCTTGTT CAATACAGAG ACCGCAGTAA ATCCGAAGAC CACTCTGAGA 
CGCAGAAGGA CTGTGATTGG ATTCTCCCAC CATCAGTCAC AGGATCACGT AGACAAAACT 
CCATTGGCAG AGGCAACCCC CAAGCCAAGC ACATCAGGCA ATGTCAACGG TGTAACGTCT 
ATTGAACCTC CGCCAGATAG AGAAGAGTCC CCACCTCCTA AGCCTGAAGC ATTTCATGGG 
AGACATCCTG GAGGAAGAGC AGCTACTCCC CATCCTCAGC AGCTTCCTTT AAGAAAGACG 
TTTAGTGACC TTGGGCATAT TTTGCAGTCG CCAAGCAACA TGGACAATGC AGGTGTTATG 
TATGCTAAAC CTCCATGCAA TGGAGCGAAA GACTCTTCTA TGTCTTTAAA CTGGCAGGAA 
AGCTCATTTA ACTACATGAG TCCATCTAGC CCAGTCAAAA GCCAGCACAG TAGTGAAACA 
ACAAAACCTT CCACCTTATC AATGGCTTCT CCTGGAATGT CTAAAGGTGC AGGATCGTAT 
AAGCACAGAA AAGAGAACAT AGTGAGGAAT AACAGTGGAA ATATGTGTTC TAGTGCTACT 
CTTCCAGCCC CCACAGTAAA TCACACAAAA ACTGAAGAGT TAAAAGGCAT CTTTCACACT 
GGTCAAACAG GGGGAATCAA CTCGTCAACA TTGCCTACAC TATCTAGTGA GTGCACTTTC 
CGCGAGAGGT CATTATCAAT GCCTACTGAC TCAGGTTCCC TATCCTCTGT AGATATTGCC 
TATACAGAGA ACAGGAGAGG AAGTGGGGAC TATGCCCTAA ATTATCCTAG TGCAAGCTCT 
GAAGAGAGCA CCAGTACTGA GAATGTATCT GTTTCAGCAG AGCAAGACAA CCAGGAAAGG 
AGGAGATCTC GGAGCATCTC TTTGAGAAAG CCAAAAAAGA AGCCATCACC TCCTGCTCGC 
AGTGTTTCGT TGATTAAAGA TGCAAGTGGC CAGAAATTAC AACCGGTAGC CACCATGCCA 
AAGGACCAAC GGCCTAAAAG TCTCTGTATT GCCTTTGATA GCCAAGGGCA CAGTTATATT 
CAAACATCCA TGCATGGTAA TGCAACAATG CCAACATCAA AAAGCTTGCA CAGTTGGAAC 
CTTAGTGACT GCAAATCCAA TGACTTGTAT AGGTCTCTGT CTGGCTCTAG CACAATGACA 
GGTACCACTG TGATAGAACG CATGAAGGCA CCAGGAAGTT CAGAATCATT AAGTTCTCTT 
TCTTATTCTA GGGCCACTAC CCCCTCCCAG GTTTCTACTG AGACAGACTT AAAAATGTCT 
CCTGGAAAAC CTCCTGGCCT AATGTCACCA TCAAGTGGTT ACTCTAGTCA GTCAGAGACC 
CCAACTGCAG TGCCAACATC ATTGATTCTT GGCCACTCTC CTTACCAAAG CAGTAAGGTG 
AGACCACTTG TTCCTGAGAG AAAGTCTTCA CTGCCAGCTG TTTCACCAAT TGACAGAAGC 
CCAAAAGTCC ACCAGTCTTT TGATTTACCA ATTACTCCAT CTACACATAC ACATATGGAC 
CTAACTACAC TGCGAAGGCC TTCAAAAGGC AAAATAAGGG CCAACCGAAA TGCATCTGAA 
TCAACCTTCG GTACTTCTAA ACTTAACCAA AAGACTAGTC CAGTGCAGCC CATAATGCCT 
ATGGTTACCC AGTCTGACTT GAGATCCATA CGTTTGCGAT CAGTCAGTAA ATCAGAGCCC 
GACGACAGTT TTGATGGCAT CGATCATATC GATGAACTTG CTGACGAAGC ATTCCCGTTG 
CCTGAAAAAA AAATAAAACC TCCAGTTGCA GAGAAACCCC CAATGTCCAG AAGACCAATA 
AACCTTTCCT ACAGAAACCC TGCAGTTACA CCGGAATCCC CTGTTCTATC TCCCAGTACT 
CCTGGAACAC CTGCCAGAGA TCAAGTATCG CCACAGGATG CCTACCTGGT GATTGGAACT 
ATTAGATACA GGAAAATGAA TGATACAGAA GCTCGCATAG AAACTCCCCC TTCCTTAGAC 
CCGTCACCCC CTTTCGATAG TTGCTTTCCT GCAACCCATT CAGTGGTTAG AAGTTTTTCG 
CAGAGCAGCA TTGAAGAAGA AGAGCTCTAT AAAGGTAAAC AACTGCCTGA AAGAATAACT 
CTACAAAGCT TGAACTATAT GGATTCAAAG AAAATCAAGG TTCCACCGCC TATTCCCAAA 
AAGCCCAGTG TTTTACATCT TCCAGTTTCC AGTCCACCTC CTCCTAACCA AATGGCATCC 
TCACCTTCCG AGCAACGTTC GTCGCCGATC ATTACTCTAG ACGCTGGCAC AAGCTCTTTT 
CCTGACATTA TTGAGTTCCA AAAATATCAC GGAGTTATCT CTGCTGCTCA GCCAGATGTA 
ATAACTATTG ATCCTCTGTC AGGAGCTTTT ACAGACTTAT CAGAGGAGAA CAAAAGTGTA 
TCAAACAAAA CAGCAGAATC AATCACAGAG GATGATGATG AAGTTTTCGT CAGTTCACGC 
ACAACGGAAG ACTTATTTAC GGTCATACAC AGGTCAAAAA GGAAGTTATT GGGATGGAAA 
GATTCTGGAG ATTCTTTTGG AAGCCGGCAA AGCTCAGTGT CACCGGTAAA GAATTCAGGT 
AGCTCCACAA GTAGTGACTA TTCAACTGGG ACCATGAGCA GCTCGCGGTC ATCCAGCAAA 
AATGAAGACT TCAAAGCCCT TCTCCAGAGA AAAGGCAGCA AGAGCAACAT TGGGTCTCGT 
CCTTCAGCTG CCGAACTTCT CAAGACAACT AATCCCTTGG CACGGAGGGT CTTGAACGAG 
TTTGCTCCAG AGATTGAGAA AAGTGGCAGC ACTAAAACCT TACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl2 ( XM_031891005.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746865.1 Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa14264
Clone ID Related Accession (Same CDS sequence)	XM_031891005.1
Accession Version	XM_031891005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGGA ACTCACCGCA CAAACAGAGA AGCAAGAAGC ATCACAGAAT GCAGCCGGCA 
ACAGCTGCTT CTGCTGTGGA CCAATCGAAG TTGGCAAACT GGCAACAACA GCCAGTAAAT 
GTTTTTCTGT CCACTAAGCG GCCTCCTTGC GTGGAAGAGC TTCATCAAGA GGCTGAACTG 
AACCTGCAGA GCCTTCTTCA GGCTGATTGC TTTACAGAAG AATTTGAAGA TCGCTATCTA 
GACACTAAAG TCACTGGGCA AACATTCCGC TATTCTGCCC TCTCGCAATC TGCAACACTT 
CCTCCAGAGA CTCCCACCAG GAATACCCCC AAGCGCCTTG AGTTTGTATA TCTGCCAACC 
AACAGAAGAG TTAGTGAAGA TGAAACCACC ACTGCCGGTG TTAGGCCACG GGAGACATGC 
CTGAGTTTAC CAACAACCCC AGACAAGCAA GTGGCTTGGA TTAAAGCTTT CCCATTCCCT 
ATCCCAGAGG AGAGGAGATG GCATCAATCT AACTCCATCC AGGCAAACGT TGTACCTATC 
AATGTGTCAG GGGAACTTTT TGATAAACAT GCAAACTCCC GGCATTCCTT GTTCAATACA 
GAGACCGCAG TAAATCCGAA GACCACTCTG AGACGCAGAA GGACTGTGAT TGGATTCTCC 
CACCATCAGT CACAGGATCA CGTAGACAAA ACTCCATTGG CAGAGGCAAC CCCCAAGCCA 
AGCACATCAG GCAATGTCAA CGGTGTAACG TCTATTGAAC CTCCGCCAGA TAGAGAAGAG 
TCCCCACCTC CTAAGCCTGA AGCATTTCAT GGGAGACATC CTGGAGGAAG AGCAGCTACT 
CCCCATCCTC AGCAGCTTCC TTTAAGAAAG ACGTTTAGTG ACCTTGGGCA TATTTTGCAG 
TCGCCAAGCA ACATGGACAA TGCAGGTGTT ATGTATGCTA AACCTCCATG CAATGGAGCG 
AAAGACTCTT CTATGTCTTT AAACTGGCAG GAAAGCTCAT TTAACTACAT GAGTCCATCT 
AGCCCAGTCA AAAGCCAGCA CAGTAGTGAA ACAACAAAAC CTTCCACCTT ATCAATGGCT 
TCTCCTGGAA TGTCTAAAGG TGCAGGATCG TATAAGCACA GAAAAGAGAA CATAGTGAGG 
AATAACAGTG GAAATATGTG TTCTAGTGCT ACTCTTCCAG CCCCCACAGT AAATCACACA 
AAAACTGAAG AGTTAAAAGG CATCTTTCAC ACTGGTCAAA CAGGGGGAAT CAACTCGTCA 
ACATTGCCTA CACTATCTAG TGAGTGCACT TTCCGCGAGA GGTCATTATC AATGCCTACT 
GACTCAGGTT CCCTATCCTC TGTAGATATT GCCTATACAG AGAACAGGAG AGGAAGTGGG 
GACTATGCCC TAAATTATCC TAGTGCAAGC TCTGAAGAGA GCACCAGTAC TGAGAATGTA 
TCTGTTTCAG CAGAGCAAGA CAACCAGGAA AGGAGGAGAT CTCGGAGCAT CTCTTTGAGA 
AAGCCAAAAA AGAAGCCATC ACCTCCTGCT CGCAGTGTTT CGTTGATTAA AGATGCAAGT 
GGCCAGAAAT TACAACCGGT AGCCACCATG CCAAAGGACC AACGGCCTAA AAGTCTCTGT 
ATTGCCTTTG ATAGCCAAGG GCACAGTTAT ATTCAAACAT CCATGCATGG TAATGCAACA 
ATGCCAACAT CAAAAAGCTT GCACAGTTGG AACCTTAGTG ACTGCAAATC CAATGACTTG 
TATAGGTCTC TGTCTGGCTC TAGCACAATG ACAGGTACCA CTGTGATAGA ACGCATGAAG 
GCACCAGGAA GTTCAGAATC ATTAAGTTCT CTTTCTTATT CTAGGGCCAC TACCCCCTCC 
CAGGTTTCTA CTGAGACAGA CTTAAAAATG TCTCCTGGAA AACCTCCTGG CCTAATGTCA 
CCATCAAGTG GTTACTCTAG TCAGTCAGAG ACCCCAACTG CAGTGCCAAC ATCATTGATT 
CTTGGCCACT CTCCTTACCA AAGCAGTAAG GTGAGACCAC TTGTTCCTGA GAGAAAGTCT 
TCACTGCCAG CTGTTTCACC AATTGACAGA AGCCCAAAAG TCCACCAGTC TTTTGATTTA 
CCAATTACTC CATCTACACA TACACATATG GACCTAACTA CACTGCGAAG GCCTTCAAAA 
GGCAAAATAA GGGCCAACCG AAATGCATCT GAATCAACCT TCGGTACTTC TAAACTTAAC 
CAAAAGACTA GTCCAGTGCA GCCCATAATG CCTATGGTTA CCCAGTCTGA CTTGAGATCC 
ATACGTTTGC GATCAGTCAG TAAATCAGAG CCCGACGACA GTTTTGATGG CATCGATCAT 
ATCGATGAAC TTGCTGACGA AGCATTCCCG TTGCCTGAAA AAAAAATAAA ACCTCCAGTT 
GCAGAGAAAC CCCCAATGTC CAGAAGACCA ATAAACCTTT CCTACAGAAA CCCTGCAGTT 
ACACCGGAAT CCCCTGTTCT ATCTCCCAGT ACTCCTGGAA CACCTGCCAG AGATCAAGTA 
TCGCCACAGG ATGCCTACCT GGTGATTGGA ACTATTAGAT ACAGGAAAAT GAATGATACA 
GAAGCTCGCA TAGAAACTCC CCCTTCCTTA GACCCGTCAC CCCCTTTCGA TAGTTGCTTT 
CCTGCAACCC ATTCAGTGGT TAGAAGTTTT TCGCAGAGCA GCATTGAAGA AGAAGAGCTC 
TATAAAGGTA AACAACTGCC TGAAAGAATA ACTCTACAAA GCTTGAACTA TATGGATTCA 
AAGAAAATCA AGGTTCCACC GCCTATTCCC AAAAAGCCCA GTGTTTTACA TCTTCCAGTT 
TCCAGTCCAC CTCCTCCTAA CCAAATGGCA TCCTCACCTT CCGAGCAACG TTCGTCGCCG 
ATCATTACTC TAGACGCTGG CACAAGCTCT TTTCCTGACA TTATTGAGTT CCAAAAATAT 
CACGGAGTTA TCTCTGCTGC TCAGCCAGAT GTAATAACTA TTGATCCTCT GTCAGGAGCT 
TTTACAGACT TATCAGAGGA GAACAAAAGT GTATCAAACA AAACAGCAGA ATCAATCACA 
GAGGATGATG ATGAAGTTTT CGTCAGTTCA CGCACAACGG AAGACTTATT TACGGTCATA 
CACAGGTCAA AAAGGAAGTT ATTGGGATGG AAAGATTCTG GAGATTCTTT TGGAAGCCGG 
CAAAGCTCAG TGTCACCGGT AAAGAATTCA GGTAGCTCCA CAAGTAGTGA CTATTCAACT 
GGGACCATGA GCAGCTCGCG GTCATCCAGC AAAAATGAAG ACTTCAAAGC CCTTCTCCAG 
AGAAAAGGCA GCAAGAGCAA CATTGGGTCT CGTCCTTCAG CTGCCGAACT TCTCAAGACA 
ACTAATCCCT TGGCACGGAG GGTCTTGAAC GAGTTTGCTC CAGAGATTGA GAAAAGTGGC 
AGCACTAAAA CCTTACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746865.1
CDS	858..4298
Translation	MMGNSPHKQRSKKHHRMQPATAASAVDQSKLANWQQQPVNVFLSTKRPPCVEELHQEAELNLQSLLQADCFTEEFEDRYLDTKVTGQTFRYSALSQSATLPPETPTRNTPKRLEFVYLPTNRRVSEDETTTAGVRPRETCLSLPTTPDKQVAWIKAFPFPIPEERRWHQSNSIQANVVPINVSGELFDKHANSRHSLFNTETAVNPKTTLRRRRTVIGFSHHQSQDHVDKTPLAEATPKPSTSGNVNGVTSIEPPPDREESPPPKPEAFHGRHPGGRAATPHPQQLPLRKTFSDLGHILQSPSNMDNAGVMYAKPPCNGAKDSSMSLNWQESSFNYMSPSSPVKSQHSSETTKPSTLSMASPGMSKGAGSYKHRKENIVRNNSGNMCSSATLPAPTVNHTKTEELKGIFHTGQTGGINSSTLPTLSSECTFRERSLSMPTDSGSLSSVDIAYTENRRGSGDYALNYPSASSEESTSTENVSVSAEQDNQERRRSRSISLRKPKKKPSPPARSVSLIKDASGQKLQPVATMPKDQRPKSLCIAFDSQGHSYIQTSMHGNATMPTSKSLHSWNLSDCKSNDLYRSLSGSSTMTGTTVIERMKAPGSSESLSSLSYSRATTPSQVSTETDLKMSPGKPPGLMSPSSGYSSQSETPTAVPTSLILGHSPYQSSKVRPLVPERKSSLPAVSPIDRSPKVHQSFDLPITPSTHTHMDLTTLRRPSKGKIRANRNASESTFGTSKLNQKTSPVQPIMPMVTQSDLRSIRLRSVSKSEPDDSFDGIDHIDELADEAFPLPEKKIKPPVAEKPPMSRRPINLSYRNPAVTPESPVLSPSTPGTPARDQVSPQDAYLVIGTIRYRKMNDTEARIETPPSLDPSPPFDSCFPATHSVVRSFSQSSIEEEELYKGKQLPERITLQSLNYMDSKKIKVPPPIPKKPSVLHLPVSSPPPPNQMASSPSEQRSSPIITLDAGTSSFPDIIEFQKYHGVISAAQPDVITIDPLSGAFTDLSEENKSVSNKTAESITEDDDEVFVSSRTTEDLFTVIHRSKRKLLGWKDSGDSFGSRQSSVSPVKNSGSSTSSDYSTGTMSSSRSSSKNEDFKALLQRKGSKSNIGSRPSAAELLKTTNPLARRVLNEFAPEIEKSGSTKTLP

Target ORF information:

RefSeq Version	XM_031891005.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891005.1

ORF Insert Sequence:

ATGATGGGGA ACTCACCGCA CAAACAGAGA AGCAAGAAGC ATCACAGAAT GCAGCCGGCA 
ACAGCTGCTT CTGCTGTGGA CCAATCGAAG TTGGCAAACT GGCAACAACA GCCAGTAAAT 
GTTTTTCTGT CCACTAAGCG GCCTCCTTGC GTGGAAGAGC TTCATCAAGA GGCTGAACTG 
AACCTGCAGA GCCTTCTTCA GGCTGATTGC TTTACAGAAG AATTTGAAGA TCGCTATCTA 
GACACTAAAG TCACTGGGCA AACATTCCGC TATTCTGCCC TCTCGCAATC TGCAACACTT 
CCTCCAGAGA CTCCCACCAG GAATACCCCC AAGCGCCTTG AGTTTGTATA TCTGCCAACC 
AACAGAAGAG TTAGTGAAGA TGAAACCACC ACTGCCGGTG TTAGGCCACG GGAGACATGC 
CTGAGTTTAC CAACAACCCC AGACAAGCAA GTGGCTTGGA TTAAAGCTTT CCCATTCCCT 
ATCCCAGAGG AGAGGAGATG GCATCAATCT AACTCCATCC AGGCAAACGT TGTACCTATC 
AATGTGTCAG GGGAACTTTT TGATAAACAT GCAAACTCCC GGCATTCCTT GTTCAATACA 
GAGACCGCAG TAAATCCGAA GACCACTCTG AGACGCAGAA GGACTGTGAT TGGATTCTCC 
CACCATCAGT CACAGGATCA CGTAGACAAA ACTCCATTGG CAGAGGCAAC CCCCAAGCCA 
AGCACATCAG GCAATGTCAA CGGTGTAACG TCTATTGAAC CTCCGCCAGA TAGAGAAGAG 
TCCCCACCTC CTAAGCCTGA AGCATTTCAT GGGAGACATC CTGGAGGAAG AGCAGCTACT 
CCCCATCCTC AGCAGCTTCC TTTAAGAAAG ACGTTTAGTG ACCTTGGGCA TATTTTGCAG 
TCGCCAAGCA ACATGGACAA TGCAGGTGTT ATGTATGCTA AACCTCCATG CAATGGAGCG 
AAAGACTCTT CTATGTCTTT AAACTGGCAG GAAAGCTCAT TTAACTACAT GAGTCCATCT 
AGCCCAGTCA AAAGCCAGCA CAGTAGTGAA ACAACAAAAC CTTCCACCTT ATCAATGGCT 
TCTCCTGGAA TGTCTAAAGG TGCAGGATCG TATAAGCACA GAAAAGAGAA CATAGTGAGG 
AATAACAGTG GAAATATGTG TTCTAGTGCT ACTCTTCCAG CCCCCACAGT AAATCACACA 
AAAACTGAAG AGTTAAAAGG CATCTTTCAC ACTGGTCAAA CAGGGGGAAT CAACTCGTCA 
ACATTGCCTA CACTATCTAG TGAGTGCACT TTCCGCGAGA GGTCATTATC AATGCCTACT 
GACTCAGGTT CCCTATCCTC TGTAGATATT GCCTATACAG AGAACAGGAG AGGAAGTGGG 
GACTATGCCC TAAATTATCC TAGTGCAAGC TCTGAAGAGA GCACCAGTAC TGAGAATGTA 
TCTGTTTCAG CAGAGCAAGA CAACCAGGAA AGGAGGAGAT CTCGGAGCAT CTCTTTGAGA 
AAGCCAAAAA AGAAGCCATC ACCTCCTGCT CGCAGTGTTT CGTTGATTAA AGATGCAAGT 
GGCCAGAAAT TACAACCGGT AGCCACCATG CCAAAGGACC AACGGCCTAA AAGTCTCTGT 
ATTGCCTTTG ATAGCCAAGG GCACAGTTAT ATTCAAACAT CCATGCATGG TAATGCAACA 
ATGCCAACAT CAAAAAGCTT GCACAGTTGG AACCTTAGTG ACTGCAAATC CAATGACTTG 
TATAGGTCTC TGTCTGGCTC TAGCACAATG ACAGGTACCA CTGTGATAGA ACGCATGAAG 
GCACCAGGAA GTTCAGAATC ATTAAGTTCT CTTTCTTATT CTAGGGCCAC TACCCCCTCC 
CAGGTTTCTA CTGAGACAGA CTTAAAAATG TCTCCTGGAA AACCTCCTGG CCTAATGTCA 
CCATCAAGTG GTTACTCTAG TCAGTCAGAG ACCCCAACTG CAGTGCCAAC ATCATTGATT 
CTTGGCCACT CTCCTTACCA AAGCAGTAAG GTGAGACCAC TTGTTCCTGA GAGAAAGTCT 
TCACTGCCAG CTGTTTCACC AATTGACAGA AGCCCAAAAG TCCACCAGTC TTTTGATTTA 
CCAATTACTC CATCTACACA TACACATATG GACCTAACTA CACTGCGAAG GCCTTCAAAA 
GGCAAAATAA GGGCCAACCG AAATGCATCT GAATCAACCT TCGGTACTTC TAAACTTAAC 
CAAAAGACTA GTCCAGTGCA GCCCATAATG CCTATGGTTA CCCAGTCTGA CTTGAGATCC 
ATACGTTTGC GATCAGTCAG TAAATCAGAG CCCGACGACA GTTTTGATGG CATCGATCAT 
ATCGATGAAC TTGCTGACGA AGCATTCCCG TTGCCTGAAA AAAAAATAAA ACCTCCAGTT 
GCAGAGAAAC CCCCAATGTC CAGAAGACCA ATAAACCTTT CCTACAGAAA CCCTGCAGTT 
ACACCGGAAT CCCCTGTTCT ATCTCCCAGT ACTCCTGGAA CACCTGCCAG AGATCAAGTA 
TCGCCACAGG ATGCCTACCT GGTGATTGGA ACTATTAGAT ACAGGAAAAT GAATGATACA 
GAAGCTCGCA TAGAAACTCC CCCTTCCTTA GACCCGTCAC CCCCTTTCGA TAGTTGCTTT 
CCTGCAACCC ATTCAGTGGT TAGAAGTTTT TCGCAGAGCA GCATTGAAGA AGAAGAGCTC 
TATAAAGGTA AACAACTGCC TGAAAGAATA ACTCTACAAA GCTTGAACTA TATGGATTCA 
AAGAAAATCA AGGTTCCACC GCCTATTCCC AAAAAGCCCA GTGTTTTACA TCTTCCAGTT 
TCCAGTCCAC CTCCTCCTAA CCAAATGGCA TCCTCACCTT CCGAGCAACG TTCGTCGCCG 
ATCATTACTC TAGACGCTGG CACAAGCTCT TTTCCTGACA TTATTGAGTT CCAAAAATAT 
CACGGAGTTA TCTCTGCTGC TCAGCCAGAT GTAATAACTA TTGATCCTCT GTCAGGAGCT 
TTTACAGACT TATCAGAGGA GAACAAAAGT GTATCAAACA AAACAGCAGA ATCAATCACA 
GAGGATGATG ATGAAGTTTT CGTCAGTTCA CGCACAACGG AAGACTTATT TACGGTCATA 
CACAGGTCAA AAAGGAAGTT ATTGGGATGG AAAGATTCTG GAGATTCTTT TGGAAGCCGG 
CAAAGCTCAG TGTCACCGGT AAAGAATTCA GGTAGCTCCA CAAGTAGTGA CTATTCAACT 
GGGACCATGA GCAGCTCGCG GTCATCCAGC AAAAATGAAG ACTTCAAAGC CCTTCTCCAG 
AGAAAAGGCA GCAAGAGCAA CATTGGGTCT CGTCCTTCAG CTGCCGAACT TCTCAAGACA 
ACTAATCCCT TGGCACGGAG GGTCTTGAAC GAGTTTGCTC CAGAGATTGA GAAAAGTGGC 
AGCACTAAAA CCTTACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl2 ( XM_031891006.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746866.1 Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa14265
Clone ID Related Accession (Same CDS sequence)	XM_031891006.1
Accession Version	XM_031891006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAGG CATTCATGAA TATTTCATGG GGAAAAGCTG CTTCTGCTGT GGACCAATCG 
AAGTTGGCAA ACTGGCAACA ACAGCCAGTA AATGTTTTTC TGTCCACTAA GCGGCCTCCT 
TGCGTGGAAG AGCTTCATCA AGAGGCTGAA CTGAACCTGC AGAGCCTTCT TCAGGCTGAT 
TGCTTTACAG AAGAATTTGA AGATCGCTAT CTAGACACTA AAGTCACTGG GCAAACATTC 
CGCTATTCTG CCCTCTCGCA ATCTGCAACA CTTCCTCCAG AGACTCCCAC CAGGAATACC 
CCCAAGCGCC TTGAGTTTGT ATATCTGCCA ACCAACAGAA GAGTTAGTGA AGATGAAACC 
ACCACTGCCG GTGTTAGGCC ACGGGAGACA TGCCTGAGTT TACCAACAAC CCCAGACAAG 
CAAGTGGCTT GGATTAAAGC TTTCCCATTC CCTATCCCAG AGGAGAGGAG ATGGCATCAA 
TCTAACTCCA TCCAGGCAAA CGTTGTACCT ATCAATGTGT CAGGGGAACT TTTTGATAAA 
CATGCAAACT CCCGGCATTC CTTGTTCAAT ACAGAGACCG CAGTAAATCC GAAGACCACT 
CTGAGACGCA GAAGGACTGT GATTGGATTC TCCCACCATC AGTCACAGGA TCACGTAGAC 
AAAACTCCAT TGGCAGAGGC AACCCCCAAG CCAAGCACAT CAGGCAATGT CAACGGTGTA 
ACGTCTATTG AACCTCCGCC AGATAGAGAA GAGTCCCCAC CTCCTAAGCC TGAAGCATTT 
CATGGGAGAC ATCCTGGAGG AAGAGCAGCT ACTCCCCATC CTCAGCAGCT TCCTTTAAGA 
AAGACGTTTA GTGACCTTGG GCATATTTTG CAGTCGCCAA GCAACATGGA CAATGCAGGT 
GTTATGTATG CTAAACCTCC ATGCAATGGA GCGAAAGACT CTTCTATGTC TTTAAACTGG 
CAGGAAAGCT CATTTAACTA CATGAGTCCA TCTAGCCCAG TCAAAAGCCA GCACAGTAGT 
GAAACAACAA AACCTTCCAC CTTATCAATG GCTTCTCCTG GAATGTCTAA AGGTGCAGGA 
TCGTATAAGC ACAGAAAAGA GAACATAGTG AGGAATAACA GTGGAAATAT GTGTTCTAGT 
GCTACTCTTC CAGCCCCCAC AGTAAATCAC ACAAAAACTG AAGAGTTAAA AGGCATCTTT 
CACACTGGTC AAACAGGGGG AATCAACTCG TCAACATTGC CTACACTATC TAGTGAGTGC 
ACTTTCCGCG AGAGGTCATT ATCAATGCCT ACTGACTCAG GTTCCCTATC CTCTGTAGAT 
ATTGCCTATA CAGAGAACAG GAGAGGAAGT GGGGACTATG CCCTAAATTA TCCTAGTGCA 
AGCTCTGAAG AGAGCACCAG TACTGAGAAT GTATCTGTTT CAGCAGAGCA AGACAACCAG 
GAAAGGAGGA GATCTCGGAG CATCTCTTTG AGAAAGCCAA AAAAGAAGCC ATCACCTCCT 
GCTCGCAGTG TTTCGTTGAT TAAAGATGCA AGTGGCCAGA AATTACAACC GGTAGCCACC 
ATGCCAAAGG ACCAACGGCC TAAAAGTCTC TGTATTGCCT TTGATAGCCA AGGGCACAGT 
TATATTCAAA CATCCATGCA TGGTAATGCA ACAATGCCAA CATCAAAAAG CTTGCACAGT 
TGGAACCTTA GTGACTGCAA ATCCAATGAC TTGTATAGGT CTCTGTCTGG CTCTAGCACA 
ATGACAGGTA CCACTGTGAT AGAACGCATG AAGGCACCAG GAAGTTCAGA ATCATTAAGT 
TCTCTTTCTT ATTCTAGGGC CACTACCCCC TCCCAGGTTT CTACTGAGAC AGACTTAAAA 
ATGTCTCCTG GAAAACCTCC TGGCCTAATG TCACCATCAA GTGGTTACTC TAGTCAGTCA 
GAGACCCCAA CTGCAGTGCC AACATCATTG ATTCTTGGCC ACTCTCCTTA CCAAAGCAGT 
AAGGTGAGAC CACTTGTTCC TGAGAGAAAG TCTTCACTGC CAGCTGTTTC ACCAATTGAC 
AGAAGCCCAA AAGTCCACCA GTCTTTTGAT TTACCAATTA CTCCATCTAC ACATACACAT 
ATGGACCTAA CTACACTGCG AAGGCCTTCA AAAGGCAAAA TAAGGGCCAA CCGAAATGCA 
TCTGAATCAA CCTTCGGTAC TTCTAAACTT AACCAAAAGA CTAGTCCAGT GCAGCCCATA 
ATGCCTATGG TTACCCAGTC TGACTTGAGA TCCATACGTT TGCGATCAGT CAGTAAATCA 
GAGCCCGACG ACAGTTTTGA TGGCATCGAT CATATCGATG AACTTGCTGA CGAAGCATTC 
CCGTTGCCTG AAAAAAAAAT AAAACCTCCA GTTGCAGAGA AACCCCCAAT GTCCAGAAGA 
CCAATAAACC TTTCCTACAG AAACCCTGCA GTTACACCGG AATCCCCTGT TCTATCTCCC 
AGTACTCCTG GAACACCTGC CAGAGATCAA GTATCGCCAC AGGATGCCTA CCTGGTGATT 
GGAACTATTA GATACAGGAA AATGAATGAT ACAGAAGCTC GCATAGAAAC TCCCCCTTCC 
TTAGACCCGT CACCCCCTTT CGATAGTTGC TTTCCTGCAA CCCATTCAGT GGTTAGAAGT 
TTTTCGCAGA GCAGCATTGA AGAAGAAGAG CTCTATAAAG GTAAACAACT GCCTGAAAGA 
ATAACTCTAC AAAGCTTGAA CTATATGGAT TCAAAGAAAA TCAAGGTTCC ACCGCCTATT 
CCCAAAAAGC CCAGTGTTTT ACATCTTCCA GTTTCCAGTC CACCTCCTCC TAACCAAATG 
GCATCCTCAC CTTCCGAGCA ACGTTCGTCG CCGATCATTA CTCTAGACGC TGGCACAAGC 
TCTTTTCCTG ACATTATTGA GTTCCAAAAA TATCACGGAG TTATCTCTGC TGCTCAGCCA 
GATGTAATAA CTATTGATCC TCTGTCAGGA GCTTTTACAG ACTTATCAGA GGAGAACAAA 
AGTGTATCAA ACAAAACAGC AGAATCAATC ACAGAGGATG ATGATGAAGT TTTCGTCAGT 
TCACGCACAA CGGAAGACTT ATTTACGGTC ATACACAGGT CAAAAAGGAA GTTATTGGGA 
TGGAAAGATT CTGGAGATTC TTTTGGAAGC CGGCAAAGCT CAGTGTCACC GGTAAAGAAT 
TCAGGTAGCT CCACAAGTAG TGACTATTCA ACTGGGACCA TGAGCAGCTC GCGGTCATCC 
AGCAAAAATG AAGACTTCAA AGCCCTTCTC CAGAGAAAAG GCAGCAAGAG CAACATTGGG 
TCTCGTCCTT CAGCTGCCGA ACTTCTCAAG ACAACTAATC CCTTGGCACG GAGGGTCTTG 
AACGAGTTTG CTCCAGAGAT TGAGAAAAGT GGCAGCACTA AAACCTTACC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746866.1
CDS	415..3828
Translation	MDKAFMNISWGKAASAVDQSKLANWQQQPVNVFLSTKRPPCVEELHQEAELNLQSLLQADCFTEEFEDRYLDTKVTGQTFRYSALSQSATLPPETPTRNTPKRLEFVYLPTNRRVSEDETTTAGVRPRETCLSLPTTPDKQVAWIKAFPFPIPEERRWHQSNSIQANVVPINVSGELFDKHANSRHSLFNTETAVNPKTTLRRRRTVIGFSHHQSQDHVDKTPLAEATPKPSTSGNVNGVTSIEPPPDREESPPPKPEAFHGRHPGGRAATPHPQQLPLRKTFSDLGHILQSPSNMDNAGVMYAKPPCNGAKDSSMSLNWQESSFNYMSPSSPVKSQHSSETTKPSTLSMASPGMSKGAGSYKHRKENIVRNNSGNMCSSATLPAPTVNHTKTEELKGIFHTGQTGGINSSTLPTLSSECTFRERSLSMPTDSGSLSSVDIAYTENRRGSGDYALNYPSASSEESTSTENVSVSAEQDNQERRRSRSISLRKPKKKPSPPARSVSLIKDASGQKLQPVATMPKDQRPKSLCIAFDSQGHSYIQTSMHGNATMPTSKSLHSWNLSDCKSNDLYRSLSGSSTMTGTTVIERMKAPGSSESLSSLSYSRATTPSQVSTETDLKMSPGKPPGLMSPSSGYSSQSETPTAVPTSLILGHSPYQSSKVRPLVPERKSSLPAVSPIDRSPKVHQSFDLPITPSTHTHMDLTTLRRPSKGKIRANRNASESTFGTSKLNQKTSPVQPIMPMVTQSDLRSIRLRSVSKSEPDDSFDGIDHIDELADEAFPLPEKKIKPPVAEKPPMSRRPINLSYRNPAVTPESPVLSPSTPGTPARDQVSPQDAYLVIGTIRYRKMNDTEARIETPPSLDPSPPFDSCFPATHSVVRSFSQSSIEEEELYKGKQLPERITLQSLNYMDSKKIKVPPPIPKKPSVLHLPVSSPPPPNQMASSPSEQRSSPIITLDAGTSSFPDIIEFQKYHGVISAAQPDVITIDPLSGAFTDLSEENKSVSNKTAESITEDDDEVFVSSRTTEDLFTVIHRSKRKLLGWKDSGDSFGSRQSSVSPVKNSGSSTSSDYSTGTMSSSRSSSKNEDFKALLQRKGSKSNIGSRPSAAELLKTTNPLARRVLNEFAPEIEKSGSTKTLP

Target ORF information:

RefSeq Version	XM_031891006.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891006.1

ORF Insert Sequence:

ATGGATAAGG CATTCATGAA TATTTCATGG GGAAAAGCTG CTTCTGCTGT GGACCAATCG 
AAGTTGGCAA ACTGGCAACA ACAGCCAGTA AATGTTTTTC TGTCCACTAA GCGGCCTCCT 
TGCGTGGAAG AGCTTCATCA AGAGGCTGAA CTGAACCTGC AGAGCCTTCT TCAGGCTGAT 
TGCTTTACAG AAGAATTTGA AGATCGCTAT CTAGACACTA AAGTCACTGG GCAAACATTC 
CGCTATTCTG CCCTCTCGCA ATCTGCAACA CTTCCTCCAG AGACTCCCAC CAGGAATACC 
CCCAAGCGCC TTGAGTTTGT ATATCTGCCA ACCAACAGAA GAGTTAGTGA AGATGAAACC 
ACCACTGCCG GTGTTAGGCC ACGGGAGACA TGCCTGAGTT TACCAACAAC CCCAGACAAG 
CAAGTGGCTT GGATTAAAGC TTTCCCATTC CCTATCCCAG AGGAGAGGAG ATGGCATCAA 
TCTAACTCCA TCCAGGCAAA CGTTGTACCT ATCAATGTGT CAGGGGAACT TTTTGATAAA 
CATGCAAACT CCCGGCATTC CTTGTTCAAT ACAGAGACCG CAGTAAATCC GAAGACCACT 
CTGAGACGCA GAAGGACTGT GATTGGATTC TCCCACCATC AGTCACAGGA TCACGTAGAC 
AAAACTCCAT TGGCAGAGGC AACCCCCAAG CCAAGCACAT CAGGCAATGT CAACGGTGTA 
ACGTCTATTG AACCTCCGCC AGATAGAGAA GAGTCCCCAC CTCCTAAGCC TGAAGCATTT 
CATGGGAGAC ATCCTGGAGG AAGAGCAGCT ACTCCCCATC CTCAGCAGCT TCCTTTAAGA 
AAGACGTTTA GTGACCTTGG GCATATTTTG CAGTCGCCAA GCAACATGGA CAATGCAGGT 
GTTATGTATG CTAAACCTCC ATGCAATGGA GCGAAAGACT CTTCTATGTC TTTAAACTGG 
CAGGAAAGCT CATTTAACTA CATGAGTCCA TCTAGCCCAG TCAAAAGCCA GCACAGTAGT 
GAAACAACAA AACCTTCCAC CTTATCAATG GCTTCTCCTG GAATGTCTAA AGGTGCAGGA 
TCGTATAAGC ACAGAAAAGA GAACATAGTG AGGAATAACA GTGGAAATAT GTGTTCTAGT 
GCTACTCTTC CAGCCCCCAC AGTAAATCAC ACAAAAACTG AAGAGTTAAA AGGCATCTTT 
CACACTGGTC AAACAGGGGG AATCAACTCG TCAACATTGC CTACACTATC TAGTGAGTGC 
ACTTTCCGCG AGAGGTCATT ATCAATGCCT ACTGACTCAG GTTCCCTATC CTCTGTAGAT 
ATTGCCTATA CAGAGAACAG GAGAGGAAGT GGGGACTATG CCCTAAATTA TCCTAGTGCA 
AGCTCTGAAG AGAGCACCAG TACTGAGAAT GTATCTGTTT CAGCAGAGCA AGACAACCAG 
GAAAGGAGGA GATCTCGGAG CATCTCTTTG AGAAAGCCAA AAAAGAAGCC ATCACCTCCT 
GCTCGCAGTG TTTCGTTGAT TAAAGATGCA AGTGGCCAGA AATTACAACC GGTAGCCACC 
ATGCCAAAGG ACCAACGGCC TAAAAGTCTC TGTATTGCCT TTGATAGCCA AGGGCACAGT 
TATATTCAAA CATCCATGCA TGGTAATGCA ACAATGCCAA CATCAAAAAG CTTGCACAGT 
TGGAACCTTA GTGACTGCAA ATCCAATGAC TTGTATAGGT CTCTGTCTGG CTCTAGCACA 
ATGACAGGTA CCACTGTGAT AGAACGCATG AAGGCACCAG GAAGTTCAGA ATCATTAAGT 
TCTCTTTCTT ATTCTAGGGC CACTACCCCC TCCCAGGTTT CTACTGAGAC AGACTTAAAA 
ATGTCTCCTG GAAAACCTCC TGGCCTAATG TCACCATCAA GTGGTTACTC TAGTCAGTCA 
GAGACCCCAA CTGCAGTGCC AACATCATTG ATTCTTGGCC ACTCTCCTTA CCAAAGCAGT 
AAGGTGAGAC CACTTGTTCC TGAGAGAAAG TCTTCACTGC CAGCTGTTTC ACCAATTGAC 
AGAAGCCCAA AAGTCCACCA GTCTTTTGAT TTACCAATTA CTCCATCTAC ACATACACAT 
ATGGACCTAA CTACACTGCG AAGGCCTTCA AAAGGCAAAA TAAGGGCCAA CCGAAATGCA 
TCTGAATCAA CCTTCGGTAC TTCTAAACTT AACCAAAAGA CTAGTCCAGT GCAGCCCATA 
ATGCCTATGG TTACCCAGTC TGACTTGAGA TCCATACGTT TGCGATCAGT CAGTAAATCA 
GAGCCCGACG ACAGTTTTGA TGGCATCGAT CATATCGATG AACTTGCTGA CGAAGCATTC 
CCGTTGCCTG AAAAAAAAAT AAAACCTCCA GTTGCAGAGA AACCCCCAAT GTCCAGAAGA 
CCAATAAACC TTTCCTACAG AAACCCTGCA GTTACACCGG AATCCCCTGT TCTATCTCCC 
AGTACTCCTG GAACACCTGC CAGAGATCAA GTATCGCCAC AGGATGCCTA CCTGGTGATT 
GGAACTATTA GATACAGGAA AATGAATGAT ACAGAAGCTC GCATAGAAAC TCCCCCTTCC 
TTAGACCCGT CACCCCCTTT CGATAGTTGC TTTCCTGCAA CCCATTCAGT GGTTAGAAGT 
TTTTCGCAGA GCAGCATTGA AGAAGAAGAG CTCTATAAAG GTAAACAACT GCCTGAAAGA 
ATAACTCTAC AAAGCTTGAA CTATATGGAT TCAAAGAAAA TCAAGGTTCC ACCGCCTATT 
CCCAAAAAGC CCAGTGTTTT ACATCTTCCA GTTTCCAGTC CACCTCCTCC TAACCAAATG 
GCATCCTCAC CTTCCGAGCA ACGTTCGTCG CCGATCATTA CTCTAGACGC TGGCACAAGC 
TCTTTTCCTG ACATTATTGA GTTCCAAAAA TATCACGGAG TTATCTCTGC TGCTCAGCCA 
GATGTAATAA CTATTGATCC TCTGTCAGGA GCTTTTACAG ACTTATCAGA GGAGAACAAA 
AGTGTATCAA ACAAAACAGC AGAATCAATC ACAGAGGATG ATGATGAAGT TTTCGTCAGT 
TCACGCACAA CGGAAGACTT ATTTACGGTC ATACACAGGT CAAAAAGGAA GTTATTGGGA 
TGGAAAGATT CTGGAGATTC TTTTGGAAGC CGGCAAAGCT CAGTGTCACC GGTAAAGAAT 
TCAGGTAGCT CCACAAGTAG TGACTATTCA ACTGGGACCA TGAGCAGCTC GCGGTCATCC 
AGCAAAAATG AAGACTTCAA AGCCCTTCTC CAGAGAAAAG GCAGCAAGAG CAACATTGGG 
TCTCGTCCTT CAGCTGCCGA ACTTCTCAAG ACAACTAATC CCTTGGCACG GAGGGTCTTG 
AACGAGTTTG CTCCAGAGAT TGAGAAAAGT GGCAGCACTA AAACCTTACC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl2 ( XM_004916790.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916847.2 Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa64261
Clone ID Related Accession (Same CDS sequence)	XM_004916790.4
Accession Version	XM_004916790.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916790.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCT ACAAGAGGAC ACTTAGGCCC CAGTCTTCCT GGGGAAGAAG TCGTCACAGT 
TCATGGTGTG TTGACACATC CCAATGTTTA ACTTCCCAGA ATGAAGGAGC CTGGGGGTAT 
CATGGACACA AACCAAGGAC TGACAATGAT AATAAAGAAG CTACCCCAGA AGAGATTGAG 
ACTGTGCGTG CCCAAGTTAT CCATGTCCAA CCTGGTTATG TTATCCCCTT TCCAACATGG 
GTTCCTAAAG CAGAGACAGG CCCCAGGGGT CACACAAAAA CATTGGTGCC AAGTAAACCC 
ACAGGTGCAA CAGCTATGTC TGTTCAGGTC CAGAATTTTA CAGATGTTTG CCTCCGCTGC 
TTGGTAACTG TTCTCCTCCA GCTCTCTGAT CTGTGCCGCC TCTCGCTGTC ACTCTGTGAG 
GAGCTGGAAG AGGAGCTGCT TGTTCTGCAC AAACGTACCG CAACCCTCCA ACACCGAACT 
GCAGCCACCT ACATAACTAT CTGTAGGGCG CGCCAGCGAG AACTAACAAC CCAGCACAGC 
AGCATCGTAC TAGCTGCTTC TGCTGTGGAC CAATCGAAGT TGGCAAACTG GCAACAACAG 
CCAGTAAATG TTTTTCTGTC CACTAAGCGG CCTCCTTGCG TGGAAGAGCT TCATCAAGAG 
GCTGAACTGA ACCTGCAGAG CCTTCTTCAG GCTGATTGCT TTACAGAAGA ATTTGAAGAT 
CGCTATCTAG ACACTAAAGT CACTGGGCAA ACATTCCGCT ATTCTGCCCT CTCGCAATCT 
GCAACACTTC CTCCAGAGAC TCCCACCAGG AATACCCCCA AGCGCCTTGA GTTTGTATAT 
CTGCCAACCA ACAGAAGAGT TAGTGAAGAT GAAACCACCA CTGCCGGTGT TAGGCCACGG 
GAGACATGCC TGAGTTTACC AACAACCCCA GACAAGCAAG TGGCTTGGAT TAAAGCTTTC 
CCATTCCCTA TCCCAGAGGA GAGGAGATGG CATCAATCTA ACTCCATCCA GGCAAACGTT 
GTACCTATCA ATGTGTCAGG GGAACTTTTT GATAAACATG CAAACTCCCG GCATTCCTTG 
TTCAATACAG AGACCGCAGT AAATCCGAAG ACCACTCTGA GACGCAGAAG GACTGTGATT 
GGATTCTCCC ACCATCAGTC ACAGGATCAC GTAGACAAAA CTCCATTGGC AGAGGCAACC 
CCCAAGCCAA GCACATCAGG CAATGTCAAC GGTGTAACGT CTATTGAACC TCCGCCAGAT 
AGAGAAGAGT CCCCACCTCC TAAGCCTGAA GCATTTCATG GGAGACATCC TGGAGGAAGA 
GCAGCTACTC CCCATCCTCA GCAGCTTCCT TTAAGAAAGA CGTTTAGTGA CCTTGGGCAT 
ATTTTGCAGT CGCCAAGCAA CATGGACAAT GCAGGTGTTA TGTATGCTAA ACCTCCATGC 
AATGGAGCGA AAGACTCTTC TATGTCTTTA AACTGGCAGG AAAGCTCATT TAACTACATG 
AGTCCATCTA GCCCAGTCAA AAGCCAGCAC AGTAGTGAAA CAACAAAACC TTCCACCTTA 
TCAATGGCTT CTCCTGGAAT GTCTAAAGGT GCAGGATCGT ATAAGCACAG AAAAGAGAAC 
ATAGTGAGGA ATAACAGTGG AAATATGTGT TCTAGTGCTA CTCTTCCAGC CCCCACAGTA 
AATCACACAA AAACTGAAGA GTTAAAAGGC ATCTTTCACA CTGGTCAAAC AGGGGGAATC 
AACTCGTCAA CATTGCCTAC ACTATCTAGT GAGTGCACTT TCCGCGAGAG GTCATTATCA 
ATGCCTACTG ACTCAGGTTC CCTATCCTCT GTAGATATTG CCTATACAGA GAACAGGAGA 
GGAAGTGGGG ACTATGCCCT AAATTATCCT AGTGCAAGCT CTGAAGAGAG CACCAGTACT 
GAGAATGTAT CTGTTTCAGC AGAGCAAGAC AACCAGGAAA GGAGGAGATC TCGGAGCATC 
TCTTTGAGAA AGCCAAAAAA GAAGCCATCA CCTCCTGCTC GCAGTGTTTC GTTGATTAAA 
GATGCAAGTG GCCAGAAATT ACAACCGGTA GCCACCATGC CAAAGGACCA ACGGCCTAAA 
AGTCTCTGTA TTGCCTTTGA TAGCCAAGGG CACAGTTATA TTCAAACATC CATGCATGGT 
AATGCAACAA TGCCAACATC AAAAAGCTTG CACAGTTGGA ACCTTAGTGA CTGCAAATCC 
AATGACTTGT ATAGGTCTCT GTCTGGCTCT AGCACAATGA CAGGTACCAC TGTGATAGAA 
CGCATGAAGG CACCAGGAAG TTCAGAATCA TTAAGTTCTC TTTCTTATTC TAGGGCCACT 
ACCCCCTCCC AGGTTTCTAC TGAGACAGAC TTAAAAATGT CTCCTGGAAA ACCTCCTGGC 
CTAATGTCAC CATCAAGTGG TTACTCTAGT CAGTCAGAGA CCCCAACTGC AGTGCCAACA 
TCATTGATTC TTGGCCACTC TCCTTACCAA AGCAGTAAGG TGAGACCACT TGTTCCTGAG 
AGAAAGTCTT CACTGCCAGC TGTTTCACCA ATTGACAGAA GCCCAAAAGT CCACCAGTCT 
TTTGATTTAC CAATTACTCC ATCTACACAT ACACATATGG ACCTAACTAC ACTGCGAAGG 
CCTTCAAAAG GCAAAATAAG GGCCAACCGA AATGCATCTG AATCAACCTT CGGTACTTCT 
AAACTTAACC AAAAGACTAG TCCAGTGCAG CCCATAATGC CTATGGTTAC CCAGTCTGAC 
TTGAGATCCA TACGTTTGCG ATCAGTCAGT AAATCAGAGC CCGACGACAG TTTTGATGGC 
ATCGATCATA TCGATGAACT TGCTGACGAA GCATTCCCGT TGCCTGAAAA AAAAATAAAA 
CCTCCAGTTG CAGAGAAACC CCCAATGTCC AGAAGACCAA TAAACCTTTC CTACAGAAAC 
CCTGCAGTTA CACCGGAATC CCCTGTTCTA TCTCCCAGTA CTCCTGGAAC ACCTGCCAGA 
GATCAAGTAT CGCCACAGGA TGCCTACCTG GTGATTGGAA CTATTAGATA CAGGAAAATG 
AATGATACAG AAGCTCGCAT AGAAACTCCC CCTTCCTTAG ACCCGTCACC CCCTTTCGAT 
AGTTGCTTTC CTGCAACCCA TTCAGTGGTT AGAAGTTTTT CGCAGAGCAG CATTGAAGAA 
GAAGAGCTCT ATAAAGGTAA ACAACTGCCT GAAAGAATAA CTCTACAAAG CTTGAACTAT 
ATGGATTCAA AGAAAATCAA GGTTCCACCG CCTATTCCCA AAAAGCCCAG TGTTTTACAT 
CTTCCAGTTT CCAGTCCACC TCCTCCTAAC CAAATGGCAT CCTCACCTTC CGAGCAACGT 
TCGTCGCCGA TCATTACTCT AGACGCTGGC ACAAGCTCTT TTCCTGACAT TATTGAGTTC 
CAAAAATATC ACGGAGTTAT CTCTGCTGCT CAGCCAGATG TAATAACTAT TGATCCTCTG 
TCAGGAGCTT TTACAGACTT ATCAGAGGAG AACAAAAGTG TATCAAACAA AACAGCAGAA 
TCAATCACAG AGGATGATGA TGAAGTTTTC GTCAGTTCAC GCACAACGGA AGACTTATTT 
ACGGTCATAC ACAGGTCAAA AAGGAAGTTA TTGGGATGGA AAGATTCTGG AGATTCTTTT 
GGAAGCCGGC AAAGCTCAGT GTCACCGGTA AAGAATTCAG GTAGCTCCAC AAGTAGTGAC 
TATTCAACTG GGACCATGAG CAGCTCGCGG TCATCCAGCA AAAATGAAGA CTTCAAAGCC 
CTTCTCCAGA GAAAAGGCAG CAAGAGCAAC ATTGGGTCTC GTCCTTCAGC TGCCGAACTT 
CTCAAGACAA CTAATCCCTT GGCACGGAGG GTCTTGAACG AGTTTGCTCC AGAGATTGAG 
AAAAGTGGCA GCACTAAAAC CTTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916847.2
CDS	568..4497
Translation	MPFYKRTLRPQSSWGRSRHSSWCVDTSQCLTSQNEGAWGYHGHKPRTDNDNKEATPEEIETVRAQVIHVQPGYVIPFPTWVPKAETGPRGHTKTLVPSKPTGATAMSVQVQNFTDVCLRCLVTVLLQLSDLCRLSLSLCEELEEELLVLHKRTATLQHRTAATYITICRARQRELTTQHSSIVLAASAVDQSKLANWQQQPVNVFLSTKRPPCVEELHQEAELNLQSLLQADCFTEEFEDRYLDTKVTGQTFRYSALSQSATLPPETPTRNTPKRLEFVYLPTNRRVSEDETTTAGVRPRETCLSLPTTPDKQVAWIKAFPFPIPEERRWHQSNSIQANVVPINVSGELFDKHANSRHSLFNTETAVNPKTTLRRRRTVIGFSHHQSQDHVDKTPLAEATPKPSTSGNVNGVTSIEPPPDREESPPPKPEAFHGRHPGGRAATPHPQQLPLRKTFSDLGHILQSPSNMDNAGVMYAKPPCNGAKDSSMSLNWQESSFNYMSPSSPVKSQHSSETTKPSTLSMASPGMSKGAGSYKHRKENIVRNNSGNMCSSATLPAPTVNHTKTEELKGIFHTGQTGGINSSTLPTLSSECTFRERSLSMPTDSGSLSSVDIAYTENRRGSGDYALNYPSASSEESTSTENVSVSAEQDNQERRRSRSISLRKPKKKPSPPARSVSLIKDASGQKLQPVATMPKDQRPKSLCIAFDSQGHSYIQTSMHGNATMPTSKSLHSWNLSDCKSNDLYRSLSGSSTMTGTTVIERMKAPGSSESLSSLSYSRATTPSQVSTETDLKMSPGKPPGLMSPSSGYSSQSETPTAVPTSLILGHSPYQSSKVRPLVPERKSSLPAVSPIDRSPKVHQSFDLPITPSTHTHMDLTTLRRPSKGKIRANRNASESTFGTSKLNQKTSPVQPIMPMVTQSDLRSIRLRSVSKSEPDDSFDGIDHIDELADEAFPLPEKKIKPPVAEKPPMSRRPINLSYRNPAVTPESPVLSPSTPGTPARDQVSPQDAYLVIGTIRYRKMNDTEARIETPPSLDPSPPFDSCFPATHSVVRSFSQSSIEEEELYKGKQLPERITLQSLNYMDSKKIKVPPPIPKKPSVLHLPVSSPPPPNQMASSPSEQRSSPIITLDAGTSSFPDIIEFQKYHGVISAAQPDVITIDPLSGAFTDLSEENKSVSNKTAESITEDDDEVFVSSRTTEDLFTVIHRSKRKLLGWKDSGDSFGSRQSSVSPVKNSGSSTSSDYSTGTMSSSRSSSKNEDFKALLQRKGSKSNIGSRPSAAELLKTTNPLARRVLNEFAPEIEKSGSTKTLP

Target ORF information:

RefSeq Version	XM_004916790.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916790.4

ORF Insert Sequence:

ATGCCTTTCT ACAAGAGGAC ACTTAGGCCC CAGTCTTCCT GGGGAAGAAG TCGTCACAGT 
TCATGGTGTG TTGACACATC CCAATGTTTA ACTTCCCAGA ATGAAGGAGC CTGGGGGTAT 
CATGGACACA AACCAAGGAC TGACAATGAT AATAAAGAAG CTACCCCAGA AGAGATTGAG 
ACTGTGCGTG CCCAAGTTAT CCATGTCCAA CCTGGTTATG TTATCCCCTT TCCAACATGG 
GTTCCTAAAG CAGAGACAGG CCCCAGGGGT CACACAAAAA CATTGGTGCC AAGTAAACCC 
ACAGGTGCAA CAGCTATGTC TGTTCAGGTC CAGAATTTTA CAGATGTTTG CCTCCGCTGC 
TTGGTAACTG TTCTCCTCCA GCTCTCTGAT CTGTGCCGCC TCTCGCTGTC ACTCTGTGAG 
GAGCTGGAAG AGGAGCTGCT TGTTCTGCAC AAACGTACCG CAACCCTCCA ACACCGAACT 
GCAGCCACCT ACATAACTAT CTGTAGGGCG CGCCAGCGAG AACTAACAAC CCAGCACAGC 
AGCATCGTAC TAGCTGCTTC TGCTGTGGAC CAATCGAAGT TGGCAAACTG GCAACAACAG 
CCAGTAAATG TTTTTCTGTC CACTAAGCGG CCTCCTTGCG TGGAAGAGCT TCATCAAGAG 
GCTGAACTGA ACCTGCAGAG CCTTCTTCAG GCTGATTGCT TTACAGAAGA ATTTGAAGAT 
CGCTATCTAG ACACTAAAGT CACTGGGCAA ACATTCCGCT ATTCTGCCCT CTCGCAATCT 
GCAACACTTC CTCCAGAGAC TCCCACCAGG AATACCCCCA AGCGCCTTGA GTTTGTATAT 
CTGCCAACCA ACAGAAGAGT TAGTGAAGAT GAAACCACCA CTGCCGGTGT TAGGCCACGG 
GAGACATGCC TGAGTTTACC AACAACCCCA GACAAGCAAG TGGCTTGGAT TAAAGCTTTC 
CCATTCCCTA TCCCAGAGGA GAGGAGATGG CATCAATCTA ACTCCATCCA GGCAAACGTT 
GTACCTATCA ATGTGTCAGG GGAACTTTTT GATAAACATG CAAACTCCCG GCATTCCTTG 
TTCAATACAG AGACCGCAGT AAATCCGAAG ACCACTCTGA GACGCAGAAG GACTGTGATT 
GGATTCTCCC ACCATCAGTC ACAGGATCAC GTAGACAAAA CTCCATTGGC AGAGGCAACC 
CCCAAGCCAA GCACATCAGG CAATGTCAAC GGTGTAACGT CTATTGAACC TCCGCCAGAT 
AGAGAAGAGT CCCCACCTCC TAAGCCTGAA GCATTTCATG GGAGACATCC TGGAGGAAGA 
GCAGCTACTC CCCATCCTCA GCAGCTTCCT TTAAGAAAGA CGTTTAGTGA CCTTGGGCAT 
ATTTTGCAGT CGCCAAGCAA CATGGACAAT GCAGGTGTTA TGTATGCTAA ACCTCCATGC 
AATGGAGCGA AAGACTCTTC TATGTCTTTA AACTGGCAGG AAAGCTCATT TAACTACATG 
AGTCCATCTA GCCCAGTCAA AAGCCAGCAC AGTAGTGAAA CAACAAAACC TTCCACCTTA 
TCAATGGCTT CTCCTGGAAT GTCTAAAGGT GCAGGATCGT ATAAGCACAG AAAAGAGAAC 
ATAGTGAGGA ATAACAGTGG AAATATGTGT TCTAGTGCTA CTCTTCCAGC CCCCACAGTA 
AATCACACAA AAACTGAAGA GTTAAAAGGC ATCTTTCACA CTGGTCAAAC AGGGGGAATC 
AACTCGTCAA CATTGCCTAC ACTATCTAGT GAGTGCACTT TCCGCGAGAG GTCATTATCA 
ATGCCTACTG ACTCAGGTTC CCTATCCTCT GTAGATATTG CCTATACAGA GAACAGGAGA 
GGAAGTGGGG ACTATGCCCT AAATTATCCT AGTGCAAGCT CTGAAGAGAG CACCAGTACT 
GAGAATGTAT CTGTTTCAGC AGAGCAAGAC AACCAGGAAA GGAGGAGATC TCGGAGCATC 
TCTTTGAGAA AGCCAAAAAA GAAGCCATCA CCTCCTGCTC GCAGTGTTTC GTTGATTAAA 
GATGCAAGTG GCCAGAAATT ACAACCGGTA GCCACCATGC CAAAGGACCA ACGGCCTAAA 
AGTCTCTGTA TTGCCTTTGA TAGCCAAGGG CACAGTTATA TTCAAACATC CATGCATGGT 
AATGCAACAA TGCCAACATC AAAAAGCTTG CACAGTTGGA ACCTTAGTGA CTGCAAATCC 
AATGACTTGT ATAGGTCTCT GTCTGGCTCT AGCACAATGA CAGGTACCAC TGTGATAGAA 
CGCATGAAGG CACCAGGAAG TTCAGAATCA TTAAGTTCTC TTTCTTATTC TAGGGCCACT 
ACCCCCTCCC AGGTTTCTAC TGAGACAGAC TTAAAAATGT CTCCTGGAAA ACCTCCTGGC 
CTAATGTCAC CATCAAGTGG TTACTCTAGT CAGTCAGAGA CCCCAACTGC AGTGCCAACA 
TCATTGATTC TTGGCCACTC TCCTTACCAA AGCAGTAAGG TGAGACCACT TGTTCCTGAG 
AGAAAGTCTT CACTGCCAGC TGTTTCACCA ATTGACAGAA GCCCAAAAGT CCACCAGTCT 
TTTGATTTAC CAATTACTCC ATCTACACAT ACACATATGG ACCTAACTAC ACTGCGAAGG 
CCTTCAAAAG GCAAAATAAG GGCCAACCGA AATGCATCTG AATCAACCTT CGGTACTTCT 
AAACTTAACC AAAAGACTAG TCCAGTGCAG CCCATAATGC CTATGGTTAC CCAGTCTGAC 
TTGAGATCCA TACGTTTGCG ATCAGTCAGT AAATCAGAGC CCGACGACAG TTTTGATGGC 
ATCGATCATA TCGATGAACT TGCTGACGAA GCATTCCCGT TGCCTGAAAA AAAAATAAAA 
CCTCCAGTTG CAGAGAAACC CCCAATGTCC AGAAGACCAA TAAACCTTTC CTACAGAAAC 
CCTGCAGTTA CACCGGAATC CCCTGTTCTA TCTCCCAGTA CTCCTGGAAC ACCTGCCAGA 
GATCAAGTAT CGCCACAGGA TGCCTACCTG GTGATTGGAA CTATTAGATA CAGGAAAATG 
AATGATACAG AAGCTCGCAT AGAAACTCCC CCTTCCTTAG ACCCGTCACC CCCTTTCGAT 
AGTTGCTTTC CTGCAACCCA TTCAGTGGTT AGAAGTTTTT CGCAGAGCAG CATTGAAGAA 
GAAGAGCTCT ATAAAGGTAA ACAACTGCCT GAAAGAATAA CTCTACAAAG CTTGAACTAT 
ATGGATTCAA AGAAAATCAA GGTTCCACCG CCTATTCCCA AAAAGCCCAG TGTTTTACAT 
CTTCCAGTTT CCAGTCCACC TCCTCCTAAC CAAATGGCAT CCTCACCTTC CGAGCAACGT 
TCGTCGCCGA TCATTACTCT AGACGCTGGC ACAAGCTCTT TTCCTGACAT TATTGAGTTC 
CAAAAATATC ACGGAGTTAT CTCTGCTGCT CAGCCAGATG TAATAACTAT TGATCCTCTG 
TCAGGAGCTT TTACAGACTT ATCAGAGGAG AACAAAAGTG TATCAAACAA AACAGCAGAA 
TCAATCACAG AGGATGATGA TGAAGTTTTC GTCAGTTCAC GCACAACGGA AGACTTATTT 
ACGGTCATAC ACAGGTCAAA AAGGAAGTTA TTGGGATGGA AAGATTCTGG AGATTCTTTT 
GGAAGCCGGC AAAGCTCAGT GTCACCGGTA AAGAATTCAG GTAGCTCCAC AAGTAGTGAC 
TATTCAACTG GGACCATGAG CAGCTCGCGG TCATCCAGCA AAAATGAAGA CTTCAAAGCC 
CTTCTCCAGA GAAAAGGCAG CAAGAGCAAC ATTGGGTCTC GTCCTTCAGC TGCCGAACTT 
CTCAAGACAA CTAATCCCTT GGCACGGAGG GTCTTGAACG AGTTTGCTCC AGAGATTGAG 
AAAAGTGGCA GCACTAAAAC CTTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl2 ( XM_012968902.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824356.2 Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X2, mRNA.

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CloneID	OXa64262
Clone ID Related Accession (Same CDS sequence)	XM_012968902.3
Accession Version	XM_012968902.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCT ACAAGAGGAC ACTTAGGCCC CAGTCTTCCT GGGGAAGAAG TCGTCACAGT 
TCATGGTGTG TTGACACATC CCAATGTTTA ACTTCCCAGA ATGAAGGAGC CTGGGGGTAT 
CATGGACACA AACCAAGGAC TGACAATGAT AATAAAGAAG CTACCCCAGA AGAGATTGAG 
ACTGTGCGTG CCCAAGTTAT CCATGTCCAA CCTGGTTATG TTATCCCCTT TCCAACATGG 
GTTCCTAAAG CAGAGACAGG CCCCAGGGGT CACACAAAAA CATTGGTGCC AAGTAAACCC 
ACAGGTGCAA CAGCTATGTC TGTTCAGGTC CAGAATTTTA CAGATGTTTG CCTCCGCTGC 
TTGGTAACTG TTCTCCTCCA GCTCTCTGAT CTGTGCCGCC TCTCGCTGTC ACTCTGTGAG 
GAGCTGGAAG AGGAGCTGCT TGTTCTGCAC AAACGTACCG CAACCCTCCA ACACCGAACT 
GCAGCCACCT ACATAACTAT CTGTAGGGCG CGCCAGCGAG AACTAACAAC CCAGCACAGC 
AGCATCGTAC TAGCTGCTTC TGCTGTGGAC CAATCGAAGT TGGCAAACTG GCAACAACAG 
CCAGTAAATG TTTTTCTGTC CACTAAGCGG CCTCCTTGCG TGGAAGAGCT TCATCAAGAG 
GCTGAACTGA ACCTGCAGAG CCTTCTTCAG GCTGATTGCT TTACAGAAGA ATTTGAAGAT 
CGCTATCTAG ACACTAAAGT CACTGGGCAA ACATTCCGCT ATTCTGCCCT CTCGCAATCT 
GCAACACTTC CTCCAGAGAC TCCCACCAGG AATACCCCCA AGCGCCTTGA GTTTGTATAT 
CTGCCAACCA ACAGAAGAGT TAGTGAAGAT GAAACCACCA CTGCCGGTGT TAGGCCACGG 
GAGACATGCC TGAGTTTACC AACAACCCCA GACAAGCAAG TGGCTTGGAT TAAAGCTTTC 
CCATTCCCTA TCCCAGAGGA GAGGAGATGG CATCAATCTA ACTCCATCCA GGCAAACGTT 
GTACCTATCA ATGTGTCAGA GACCGCAGTA AATCCGAAGA CCACTCTGAG ACGCAGAAGG 
ACTGTGATTG GATTCTCCCA CCATCAGTCA CAGGATCACG TAGACAAAAC TCCATTGGCA 
GAGGCAACCC CCAAGCCAAG CACATCAGGC AATGTCAACG GTGTAACGTC TATTGAACCT 
CCGCCAGATA GAGAAGAGTC CCCACCTCCT AAGCCTGAAG CATTTCATGG GAGACATCCT 
GGAGGAAGAG CAGCTACTCC CCATCCTCAG CAGCTTCCTT TAAGAAAGAC GTTTAGTGAC 
CTTGGGCATA TTTTGCAGTC GCCAAGCAAC ATGGACAATG CAGGTGTTAT GTATGCTAAA 
CCTCCATGCA ATGGAGCGAA AGACTCTTCT ATGTCTTTAA ACTGGCAGGA AAGCTCATTT 
AACTACATGA GTCCATCTAG CCCAGTCAAA AGCCAGCACA GTAGTGAAAC AACAAAACCT 
TCCACCTTAT CAATGGCTTC TCCTGGAATG TCTAAAGGTG CAGGATCGTA TAAGCACAGA 
AAAGAGAACA TAGTGAGGAA TAACAGTGGA AATATGTGTT CTAGTGCTAC TCTTCCAGCC 
CCCACAGTAA ATCACACAAA AACTGAAGAG TTAAAAGGCA TCTTTCACAC TGGTCAAACA 
GGGGGAATCA ACTCGTCAAC ATTGCCTACA CTATCTAGTG AGTGCACTTT CCGCGAGAGG 
TCATTATCAA TGCCTACTGA CTCAGGTTCC CTATCCTCTG TAGATATTGC CTATACAGAG 
AACAGGAGAG GAAGTGGGGA CTATGCCCTA AATTATCCTA GTGCAAGCTC TGAAGAGAGC 
ACCAGTACTG AGAATGTATC TGTTTCAGCA GAGCAAGACA ACCAGGAAAG GAGGAGATCT 
CGGAGCATCT CTTTGAGAAA GCCAAAAAAG AAGCCATCAC CTCCTGCTCG CAGTGTTTCG 
TTGATTAAAG ATGCAAGTGG CCAGAAATTA CAACCGGTAG CCACCATGCC AAAGGACCAA 
CGGCCTAAAA GTCTCTGTAT TGCCTTTGAT AGCCAAGGGC ACAGTTATAT TCAAACATCC 
ATGCATGGTA ATGCAACAAT GCCAACATCA AAAAGCTTGC ACAGTTGGAA CCTTAGTGAC 
TGCAAATCCA ATGACTTGTA TAGGTCTCTG TCTGGCTCTA GCACAATGAC AGGTACCACT 
GTGATAGAAC GCATGAAGGC ACCAGGAAGT TCAGAATCAT TAAGTTCTCT TTCTTATTCT 
AGGGCCACTA CCCCCTCCCA GGTTTCTACT GAGACAGACT TAAAAATGTC TCCTGGAAAA 
CCTCCTGGCC TAATGTCACC ATCAAGTGGT TACTCTAGTC AGTCAGAGAC CCCAACTGCA 
GTGCCAACAT CATTGATTCT TGGCCACTCT CCTTACCAAA GCAGTAAGGT GAGACCACTT 
GTTCCTGAGA GAAAGTCTTC ACTGCCAGCT GTTTCACCAA TTGACAGAAG CCCAAAAGTC 
CACCAGTCTT TTGATTTACC AATTACTCCA TCTACACATA CACATATGGA CCTAACTACA 
CTGCGAAGGC CTTCAAAAGG CAAAATAAGG GCCAACCGAA ATGCATCTGA ATCAACCTTC 
GGTACTTCTA AACTTAACCA AAAGACTAGT CCAGTGCAGC CCATAATGCC TATGGTTACC 
CAGTCTGACT TGAGATCCAT ACGTTTGCGA TCAGTCAGTA AATCAGAGCC CGACGACAGT 
TTTGATGGCA TCGATCATAT CGATGAACTT GCTGACGAAG CATTCCCGTT GCCTGAAAAA 
AAAATAAAAC CTCCAGTTGC AGAGAAACCC CCAATGTCCA GAAGACCAAT AAACCTTTCC 
TACAGAAACC CTGCAGTTAC ACCGGAATCC CCTGTTCTAT CTCCCAGTAC TCCTGGAACA 
CCTGCCAGAG ATCAAGTATC GCCACAGGAT GCCTACCTGG TGATTGGAAC TATTAGATAC 
AGGAAAATGA ATGATACAGA AGCTCGCATA GAAACTCCCC CTTCCTTAGA CCCGTCACCC 
CCTTTCGATA GTTGCTTTCC TGCAACCCAT TCAGTGGTTA GAAGTTTTTC GCAGAGCAGC 
ATTGAAGAAG AAGAGCTCTA TAAAGGTAAA CAACTGCCTG AAAGAATAAC TCTACAAAGC 
TTGAACTATA TGGATTCAAA GAAAATCAAG GTTCCACCGC CTATTCCCAA AAAGCCCAGT 
GTTTTACATC TTCCAGTTTC CAGTCCACCT CCTCCTAACC AAATGGCATC CTCACCTTCC 
GAGCAACGTT CGTCGCCGAT CATTACTCTA GACGCTGGCA CAAGCTCTTT TCCTGACATT 
ATTGAGTTCC AAAAATATCA CGGAGTTATC TCTGCTGCTC AGCCAGATGT AATAACTATT 
GATCCTCTGT CAGGAGCTTT TACAGACTTA TCAGAGGAGA ACAAAAGTGT ATCAAACAAA 
ACAGCAGAAT CAATCACAGA GGATGATGAT GAAGTTTTCG TCAGTTCACG CACAACGGAA 
GACTTATTTA CGGTCATACA CAGGTCAAAA AGGAAGTTAT TGGGATGGAA AGATTCTGGA 
GATTCTTTTG GAAGCCGGCA AAGCTCAGTG TCACCGGTAA AGAATTCAGG TAGCTCCACA 
AGTAGTGACT ATTCAACTGG GACCATGAGC AGCTCGCGGT CATCCAGCAA AAATGAAGAC 
TTCAAAGCCC TTCTCCAGAG AAAAGGCAGC AAGAGCAACA TTGGGTCTCG TCCTTCAGCT 
GCCGAACTTC TCAAGACAAC TAATCCCTTG GCACGGAGGG TCTTGAACGA GTTTGCTCCA 
GAGATTGAGA AAAGTGGCAG CACTAAAACC TTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824356.2
CDS	570..4448
Translation	MPFYKRTLRPQSSWGRSRHSSWCVDTSQCLTSQNEGAWGYHGHKPRTDNDNKEATPEEIETVRAQVIHVQPGYVIPFPTWVPKAETGPRGHTKTLVPSKPTGATAMSVQVQNFTDVCLRCLVTVLLQLSDLCRLSLSLCEELEEELLVLHKRTATLQHRTAATYITICRARQRELTTQHSSIVLAASAVDQSKLANWQQQPVNVFLSTKRPPCVEELHQEAELNLQSLLQADCFTEEFEDRYLDTKVTGQTFRYSALSQSATLPPETPTRNTPKRLEFVYLPTNRRVSEDETTTAGVRPRETCLSLPTTPDKQVAWIKAFPFPIPEERRWHQSNSIQANVVPINVSETAVNPKTTLRRRRTVIGFSHHQSQDHVDKTPLAEATPKPSTSGNVNGVTSIEPPPDREESPPPKPEAFHGRHPGGRAATPHPQQLPLRKTFSDLGHILQSPSNMDNAGVMYAKPPCNGAKDSSMSLNWQESSFNYMSPSSPVKSQHSSETTKPSTLSMASPGMSKGAGSYKHRKENIVRNNSGNMCSSATLPAPTVNHTKTEELKGIFHTGQTGGINSSTLPTLSSECTFRERSLSMPTDSGSLSSVDIAYTENRRGSGDYALNYPSASSEESTSTENVSVSAEQDNQERRRSRSISLRKPKKKPSPPARSVSLIKDASGQKLQPVATMPKDQRPKSLCIAFDSQGHSYIQTSMHGNATMPTSKSLHSWNLSDCKSNDLYRSLSGSSTMTGTTVIERMKAPGSSESLSSLSYSRATTPSQVSTETDLKMSPGKPPGLMSPSSGYSSQSETPTAVPTSLILGHSPYQSSKVRPLVPERKSSLPAVSPIDRSPKVHQSFDLPITPSTHTHMDLTTLRRPSKGKIRANRNASESTFGTSKLNQKTSPVQPIMPMVTQSDLRSIRLRSVSKSEPDDSFDGIDHIDELADEAFPLPEKKIKPPVAEKPPMSRRPINLSYRNPAVTPESPVLSPSTPGTPARDQVSPQDAYLVIGTIRYRKMNDTEARIETPPSLDPSPPFDSCFPATHSVVRSFSQSSIEEEELYKGKQLPERITLQSLNYMDSKKIKVPPPIPKKPSVLHLPVSSPPPPNQMASSPSEQRSSPIITLDAGTSSFPDIIEFQKYHGVISAAQPDVITIDPLSGAFTDLSEENKSVSNKTAESITEDDDEVFVSSRTTEDLFTVIHRSKRKLLGWKDSGDSFGSRQSSVSPVKNSGSSTSSDYSTGTMSSSRSSSKNEDFKALLQRKGSKSNIGSRPSAAELLKTTNPLARRVLNEFAPEIEKSGSTKTLP

Target ORF information:

RefSeq Version	XM_012968902.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968902.3

ORF Insert Sequence:

ATGCCTTTCT ACAAGAGGAC ACTTAGGCCC CAGTCTTCCT GGGGAAGAAG TCGTCACAGT 
TCATGGTGTG TTGACACATC CCAATGTTTA ACTTCCCAGA ATGAAGGAGC CTGGGGGTAT 
CATGGACACA AACCAAGGAC TGACAATGAT AATAAAGAAG CTACCCCAGA AGAGATTGAG 
ACTGTGCGTG CCCAAGTTAT CCATGTCCAA CCTGGTTATG TTATCCCCTT TCCAACATGG 
GTTCCTAAAG CAGAGACAGG CCCCAGGGGT CACACAAAAA CATTGGTGCC AAGTAAACCC 
ACAGGTGCAA CAGCTATGTC TGTTCAGGTC CAGAATTTTA CAGATGTTTG CCTCCGCTGC 
TTGGTAACTG TTCTCCTCCA GCTCTCTGAT CTGTGCCGCC TCTCGCTGTC ACTCTGTGAG 
GAGCTGGAAG AGGAGCTGCT TGTTCTGCAC AAACGTACCG CAACCCTCCA ACACCGAACT 
GCAGCCACCT ACATAACTAT CTGTAGGGCG CGCCAGCGAG AACTAACAAC CCAGCACAGC 
AGCATCGTAC TAGCTGCTTC TGCTGTGGAC CAATCGAAGT TGGCAAACTG GCAACAACAG 
CCAGTAAATG TTTTTCTGTC CACTAAGCGG CCTCCTTGCG TGGAAGAGCT TCATCAAGAG 
GCTGAACTGA ACCTGCAGAG CCTTCTTCAG GCTGATTGCT TTACAGAAGA ATTTGAAGAT 
CGCTATCTAG ACACTAAAGT CACTGGGCAA ACATTCCGCT ATTCTGCCCT CTCGCAATCT 
GCAACACTTC CTCCAGAGAC TCCCACCAGG AATACCCCCA AGCGCCTTGA GTTTGTATAT 
CTGCCAACCA ACAGAAGAGT TAGTGAAGAT GAAACCACCA CTGCCGGTGT TAGGCCACGG 
GAGACATGCC TGAGTTTACC AACAACCCCA GACAAGCAAG TGGCTTGGAT TAAAGCTTTC 
CCATTCCCTA TCCCAGAGGA GAGGAGATGG CATCAATCTA ACTCCATCCA GGCAAACGTT 
GTACCTATCA ATGTGTCAGA GACCGCAGTA AATCCGAAGA CCACTCTGAG ACGCAGAAGG 
ACTGTGATTG GATTCTCCCA CCATCAGTCA CAGGATCACG TAGACAAAAC TCCATTGGCA 
GAGGCAACCC CCAAGCCAAG CACATCAGGC AATGTCAACG GTGTAACGTC TATTGAACCT 
CCGCCAGATA GAGAAGAGTC CCCACCTCCT AAGCCTGAAG CATTTCATGG GAGACATCCT 
GGAGGAAGAG CAGCTACTCC CCATCCTCAG CAGCTTCCTT TAAGAAAGAC GTTTAGTGAC 
CTTGGGCATA TTTTGCAGTC GCCAAGCAAC ATGGACAATG CAGGTGTTAT GTATGCTAAA 
CCTCCATGCA ATGGAGCGAA AGACTCTTCT ATGTCTTTAA ACTGGCAGGA AAGCTCATTT 
AACTACATGA GTCCATCTAG CCCAGTCAAA AGCCAGCACA GTAGTGAAAC AACAAAACCT 
TCCACCTTAT CAATGGCTTC TCCTGGAATG TCTAAAGGTG CAGGATCGTA TAAGCACAGA 
AAAGAGAACA TAGTGAGGAA TAACAGTGGA AATATGTGTT CTAGTGCTAC TCTTCCAGCC 
CCCACAGTAA ATCACACAAA AACTGAAGAG TTAAAAGGCA TCTTTCACAC TGGTCAAACA 
GGGGGAATCA ACTCGTCAAC ATTGCCTACA CTATCTAGTG AGTGCACTTT CCGCGAGAGG 
TCATTATCAA TGCCTACTGA CTCAGGTTCC CTATCCTCTG TAGATATTGC CTATACAGAG 
AACAGGAGAG GAAGTGGGGA CTATGCCCTA AATTATCCTA GTGCAAGCTC TGAAGAGAGC 
ACCAGTACTG AGAATGTATC TGTTTCAGCA GAGCAAGACA ACCAGGAAAG GAGGAGATCT 
CGGAGCATCT CTTTGAGAAA GCCAAAAAAG AAGCCATCAC CTCCTGCTCG CAGTGTTTCG 
TTGATTAAAG ATGCAAGTGG CCAGAAATTA CAACCGGTAG CCACCATGCC AAAGGACCAA 
CGGCCTAAAA GTCTCTGTAT TGCCTTTGAT AGCCAAGGGC ACAGTTATAT TCAAACATCC 
ATGCATGGTA ATGCAACAAT GCCAACATCA AAAAGCTTGC ACAGTTGGAA CCTTAGTGAC 
TGCAAATCCA ATGACTTGTA TAGGTCTCTG TCTGGCTCTA GCACAATGAC AGGTACCACT 
GTGATAGAAC GCATGAAGGC ACCAGGAAGT TCAGAATCAT TAAGTTCTCT TTCTTATTCT 
AGGGCCACTA CCCCCTCCCA GGTTTCTACT GAGACAGACT TAAAAATGTC TCCTGGAAAA 
CCTCCTGGCC TAATGTCACC ATCAAGTGGT TACTCTAGTC AGTCAGAGAC CCCAACTGCA 
GTGCCAACAT CATTGATTCT TGGCCACTCT CCTTACCAAA GCAGTAAGGT GAGACCACTT 
GTTCCTGAGA GAAAGTCTTC ACTGCCAGCT GTTTCACCAA TTGACAGAAG CCCAAAAGTC 
CACCAGTCTT TTGATTTACC AATTACTCCA TCTACACATA CACATATGGA CCTAACTACA 
CTGCGAAGGC CTTCAAAAGG CAAAATAAGG GCCAACCGAA ATGCATCTGA ATCAACCTTC 
GGTACTTCTA AACTTAACCA AAAGACTAGT CCAGTGCAGC CCATAATGCC TATGGTTACC 
CAGTCTGACT TGAGATCCAT ACGTTTGCGA TCAGTCAGTA AATCAGAGCC CGACGACAGT 
TTTGATGGCA TCGATCATAT CGATGAACTT GCTGACGAAG CATTCCCGTT GCCTGAAAAA 
AAAATAAAAC CTCCAGTTGC AGAGAAACCC CCAATGTCCA GAAGACCAAT AAACCTTTCC 
TACAGAAACC CTGCAGTTAC ACCGGAATCC CCTGTTCTAT CTCCCAGTAC TCCTGGAACA 
CCTGCCAGAG ATCAAGTATC GCCACAGGAT GCCTACCTGG TGATTGGAAC TATTAGATAC 
AGGAAAATGA ATGATACAGA AGCTCGCATA GAAACTCCCC CTTCCTTAGA CCCGTCACCC 
CCTTTCGATA GTTGCTTTCC TGCAACCCAT TCAGTGGTTA GAAGTTTTTC GCAGAGCAGC 
ATTGAAGAAG AAGAGCTCTA TAAAGGTAAA CAACTGCCTG AAAGAATAAC TCTACAAAGC 
TTGAACTATA TGGATTCAAA GAAAATCAAG GTTCCACCGC CTATTCCCAA AAAGCCCAGT 
GTTTTACATC TTCCAGTTTC CAGTCCACCT CCTCCTAACC AAATGGCATC CTCACCTTCC 
GAGCAACGTT CGTCGCCGAT CATTACTCTA GACGCTGGCA CAAGCTCTTT TCCTGACATT 
ATTGAGTTCC AAAAATATCA CGGAGTTATC TCTGCTGCTC AGCCAGATGT AATAACTATT 
GATCCTCTGT CAGGAGCTTT TACAGACTTA TCAGAGGAGA ACAAAAGTGT ATCAAACAAA 
ACAGCAGAAT CAATCACAGA GGATGATGAT GAAGTTTTCG TCAGTTCACG CACAACGGAA 
GACTTATTTA CGGTCATACA CAGGTCAAAA AGGAAGTTAT TGGGATGGAA AGATTCTGGA 
GATTCTTTTG GAAGCCGGCA AAGCTCAGTG TCACCGGTAA AGAATTCAGG TAGCTCCACA 
AGTAGTGACT ATTCAACTGG GACCATGAGC AGCTCGCGGT CATCCAGCAA AAATGAAGAC 
TTCAAAGCCC TTCTCCAGAG AAAAGGCAGC AAGAGCAACA TTGGGTCTCG TCCTTCAGCT 
GCCGAACTTC TCAAGACAAC TAATCCCTTG GCACGGAGGG TCTTGAACGA GTTTGCTCCA 
GAGATTGAGA AAAGTGGCAG CACTAAAACC TTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl2 ( XM_004916790.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916847.1 Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X1, mRNA.

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CloneID	OXa14261
Clone ID Related Accession (Same CDS sequence)	XM_004916790.3
Accession Version	XM_004916790.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCT ACAAGAGGAC ACTTAGGCCC CAGTCTTCCT GGGGAAGAAG TCGTCACAGT 
TCATGGTGTG TTGACACATC CCAATGTTTA ACTTCCCAGA ATGAAGGAGC CTGGGGGCAT 
CATGGACACA AACCAAGGAC TGACAATGAT AATAAAGAAG CTACCCCAGA AGAGATTGAG 
ACTGTGCGTG CCCAAGTTAT CCATGTCCAA CCTGGTTATG TTATCCCCTT TCCAACATGG 
GTTCCTAAAG CAGAGACAGG CCCCAGGGGT CTCACAAAAA CATTGGTGCC AAGTAAACCC 
ACAGGTGCAA CAGCTATGTC TGTTCAGGTC CAGAATTTTA CAGATGTTTG CCTCCGCTGC 
TTGGTAACTG TTCTCCTCCA GCTCTCTGAT CTGTGCCGCC TCTCGCTGTC ACTCTGTGAG 
GAGCTGGAAG AGGAGCTGCT TGTTCTGCAC AAACGTACCG CAGCCCTCCA ACACCGAACT 
GCAGCCACCT ACATAACTAT CTGTAGGGCG CGCCAGCGAG AACTAACAAC CCAGCACAGC 
AGCATCGTAC TAGCTGCTTC TGCTGTGGAC CAATCGAAGT TGGCAAACTG GCAACAACAG 
CCAGTAAATG TTTTTCTGTC CACTAAGCGG CCTCCTTGCG TGGAAGAGCT TCATCAAGAG 
GCTGAACTGA ACCTGCAGAG CCTTCTTCAG GCTGATTGCT TTACAGAAGA ATTTGAAGAT 
CGCTATCTAG ACACTAAAGT CACTGGGCAA ACATTCCGCT ATTCTGCCCT CTCGCAATCT 
GCAACACTTC CTCCAGAGAC TCCCACCAGG AATACCCCCA AGCGCCTTGA GTTTGTATAT 
CTGCCAACCA ACAGAAGAGT TAGTGAAGAT GAAACCACCA CTGCCGGTGT TAGGCCACGG 
GAGACATGCC TGAGTTTACC AACAACCCCA GACAAGCAAG TGGCTTGGAT TAAAGCTTTC 
CCATTCCCTA TCCCAGAGGA GAGGAGATGG CATCAATCTA ACTCCATCCA GGCAAACGTT 
GTACCTATCA ATGTGTCAGG GGAACTTTTT GATAAACATG CAAACTCCCG GCATTCCTTG 
TTCAATACAG AGACCGCAGT AAATCCGAAG ACCACTCTGA GACGCAGAAG GACTGTGATT 
GGATTCTCCC ACCATCAGTC ACAGGATCAC GTAGACAAAA CTCCATTGGC AGAGGCAACC 
CCCAAGCCAA GCACATCAGG CAATGTCAAC GGTGTAACGT CTATTGAACC TCCGCCAGAT 
AGAGAAGAGT CCCCACCTCC TAAGCCTGAA GCATTTCATG GGAGACATCC TGGAGGAAGA 
GCAGCTACTC CCCATCCTCA GCAGCTTCCT TTAAGAAAGA CGTTTAGTGA CCTTGGGCAT 
ATTTTGCAGT CGCCAAGCAA CATGGACAAT GCAGGTGTTA TGTATGCTAA ACCTCCATGC 
AATGGAGCGA AAGACTCTTC TATGTCTTTA AACTGGCAGG AAAGCTCATT TAACTACATG 
AGTCCATCTA GCCCAGTCAA AAGCCAGCAC AGTAGTGAAA CAACAAAACC TTCCACCTTA 
TCAATGGCTT CTCCTGGAAT GTCTAAAGGT GCAGGATCGT ATAAGCACAG AAAAGAGAAC 
ATAGTGAGGA ATAACAGTGG AAATATGTGT TCTAGTGCTA CTCTTCCAGC CCCCACAGTA 
AATCACACAA AAACTGAAGA GTTAAAAGGC ATCTTTCACA CTGGTCAAAC AGGGGGAATC 
AACTCGTCAA CATTGCCTAC ACTATCTAGT GAGTGCACTT TCCGCGAGAG GTCATTATCA 
ATGCCTACTG ACTCAGGTTC CCTATCCTCT GTAGATATTG CCTATACAGA GAACAGGAGA 
GGAAGTGGGG ACTATGCCCT AAATTATCCT AGTGCAAGCT CTGAAGAGAG CACCAGTACT 
GAGAATGTAT CTGTTTCAGC AGAGCAAGAC AACCAGGAAA GGAGGAGATC TCGGAGCATC 
TCTTTGAGAA AGCCAAAAAA GAAGCCATCA CCTCCTGCTC GCAGTGTTTC GTTGATTAAA 
GATGCAAGTG GCCAGAAATT ACAACCGGTA GCCACCATGC CAAAGGACCA ACGGCCTAAA 
AGTCTCTGTA TTGCCTTTGA TAGCCAAGGG CACAGTTATA TTCAAACATC CATGCATGGT 
AATGCAACAA TGCCAACATC AAAAAGCTTG CACAGTTGGA ACCTTAGTGA CTGCAAATCC 
AATGACTTGT ATAGGTCTCT GTCTGGCTCT AGCACAATGA CAGGTACCAC TGTGATAGAA 
CGCATGAAGG CACCAGGAAG TTCAGAATCA TTAAGTTCTC TTTCTTATTC TAGGGCCACT 
ACCCCCTCCC AGGTTTCTAC TGAGACAGAC TTAAAAATGT CTCCTGGAAA ACCTCCTGGC 
CTAATGTCAC CATCAAGTGG TTACTCTAGT CAGTCAGAGA CCCCAACTGC AGTGCCAACA 
TCATTGATTC TTGGCCACTC TCCTTACCAA AGCAGTAAGG TGAGACCACT TGTTCCTGAG 
AGAAAGTCTT CACTGCCAGC TGTTTCACCA ATTGACAGAA GCCCAAAAGT CCACCAGTCT 
TTTGATTTAC CAATTACTCC ATCTACACAT ACACATATGG ACCTAACTAC ACTGCGAAGG 
CCTTCAAAAG GCAAAATAAG GGCCAACCGA AATGCATCTG AATCAACCTT CGGTACTTCT 
AAACTTAACC AAAAGACTAG TCCAGTGCAG CCCATAATGC CTATGGTTAC CCAGTCTGAC 
TTGAGATCCA TACGTTTGCG ATCAGTCAGT AAATCAGAGC CCGACGACAG TTTTGATGGC 
ATCGATCATA TCGATGAACT TGCTGACGAA GCATTCCCGT TGCCTGAAAA AAAAATAAAA 
CCTCCAGTTG CAGAGAAACC CCCAATGTCC AGAAGACCAA TAAACCTTTC CTACAGAAAC 
CCTGCAGTTA CACCGGAATC CCCTGTTCTA TCTCCCAGTA CTCCTGGAAC ACCTGCCAGA 
GATCAAGTAT CGCCACAGGA TGCCTACCTG GTGATTGGAA CTATTAGATA CAGGAAAATG 
AATGATACAG AAGCTCGCAT AGAAACTCCC CCTTCCTTAG ACCCGTCACC CCCTTTCGAT 
AGTTGCTTTC CTGCAACCCA TTCAGTGGTT AGAAGTTTTT CGCAGAGCAG CATTGAAGAA 
GAAGAGCTCT ATAAAGGTAA ACAACTGCCT GAAAGAATAA CTCTACAAAG CTTGAACTAT 
ATGGATTCAA AGAAAATCAA GGTTCCACCG CCTATTCCCA AAAAGCCCAG TGTTTTACAT 
CTTCCAGTTT CCAGTCCACC TCCTCCTAAC CAAATGGCAT CCTCACCTTC CGAGCAACGT 
TCGTCGCCGA TCATTACTCT AGACGCTGGC ACAAGCTCTT TTCCTGACAT TATTGAGTTC 
CAAAAATATC ACGGAGTTAT CTCTGCTGCT CAGCCAGATG TAATAACTAT TGATCCTCTG 
TCAGGAGCTT TTACAGACTT ATCAGAGGAG AACAAAAGTG TATCAAACAA AACAGCAGAA 
TCAATCACAG AGGATGATGA TGAAGTTTTC GTCAGTTCAC GCACAACGGA AGACTTATTT 
ACGGTCATAC ACAGGTCAAA AAGGAAGTTA TTGGGATGGA AAGATTCTGG AGATTCTTTT 
GGAAGCCGGC AAAGCTCAGT GTCACCGGTA AAGAATTCAG GTAGCTCCAC AAGTAGTGAC 
TATTCAACTG GGACCATGAG CAGCTCGCGG TCATCCAGCA AAAATGAAGA CTTCAAAGCC 
CTTCTCCAGA GAAAAGGCAG CAAGAGCAAC ATTGGGTCTC GTCCTTCAGC TGCCGAACTT 
CTCAAGACAA CTAATCCCTT GGCACGGAGG GTCTTGAACG AGTTTGCTCC AGAGATTGAG 
AAAAGTGGCA GCACTAAAAC CTTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916847.1
CDS	774..4703
Translation	MPFYKRTLRPQSSWGRSRHSSWCVDTSQCLTSQNEGAWGHHGHKPRTDNDNKEATPEEIETVRAQVIHVQPGYVIPFPTWVPKAETGPRGLTKTLVPSKPTGATAMSVQVQNFTDVCLRCLVTVLLQLSDLCRLSLSLCEELEEELLVLHKRTAALQHRTAATYITICRARQRELTTQHSSIVLAASAVDQSKLANWQQQPVNVFLSTKRPPCVEELHQEAELNLQSLLQADCFTEEFEDRYLDTKVTGQTFRYSALSQSATLPPETPTRNTPKRLEFVYLPTNRRVSEDETTTAGVRPRETCLSLPTTPDKQVAWIKAFPFPIPEERRWHQSNSIQANVVPINVSGELFDKHANSRHSLFNTETAVNPKTTLRRRRTVIGFSHHQSQDHVDKTPLAEATPKPSTSGNVNGVTSIEPPPDREESPPPKPEAFHGRHPGGRAATPHPQQLPLRKTFSDLGHILQSPSNMDNAGVMYAKPPCNGAKDSSMSLNWQESSFNYMSPSSPVKSQHSSETTKPSTLSMASPGMSKGAGSYKHRKENIVRNNSGNMCSSATLPAPTVNHTKTEELKGIFHTGQTGGINSSTLPTLSSECTFRERSLSMPTDSGSLSSVDIAYTENRRGSGDYALNYPSASSEESTSTENVSVSAEQDNQERRRSRSISLRKPKKKPSPPARSVSLIKDASGQKLQPVATMPKDQRPKSLCIAFDSQGHSYIQTSMHGNATMPTSKSLHSWNLSDCKSNDLYRSLSGSSTMTGTTVIERMKAPGSSESLSSLSYSRATTPSQVSTETDLKMSPGKPPGLMSPSSGYSSQSETPTAVPTSLILGHSPYQSSKVRPLVPERKSSLPAVSPIDRSPKVHQSFDLPITPSTHTHMDLTTLRRPSKGKIRANRNASESTFGTSKLNQKTSPVQPIMPMVTQSDLRSIRLRSVSKSEPDDSFDGIDHIDELADEAFPLPEKKIKPPVAEKPPMSRRPINLSYRNPAVTPESPVLSPSTPGTPARDQVSPQDAYLVIGTIRYRKMNDTEARIETPPSLDPSPPFDSCFPATHSVVRSFSQSSIEEEELYKGKQLPERITLQSLNYMDSKKIKVPPPIPKKPSVLHLPVSSPPPPNQMASSPSEQRSSPIITLDAGTSSFPDIIEFQKYHGVISAAQPDVITIDPLSGAFTDLSEENKSVSNKTAESITEDDDEVFVSSRTTEDLFTVIHRSKRKLLGWKDSGDSFGSRQSSVSPVKNSGSSTSSDYSTGTMSSSRSSSKNEDFKALLQRKGSKSNIGSRPSAAELLKTTNPLARRVLNEFAPEIEKSGSTKTLP

Target ORF information:

RefSeq Version	XM_004916790.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916790.3

ORF Insert Sequence:

ATGCCTTTCT ACAAGAGGAC ACTTAGGCCC CAGTCTTCCT GGGGAAGAAG TCGTCACAGT 
TCATGGTGTG TTGACACATC CCAATGTTTA ACTTCCCAGA ATGAAGGAGC CTGGGGGCAT 
CATGGACACA AACCAAGGAC TGACAATGAT AATAAAGAAG CTACCCCAGA AGAGATTGAG 
ACTGTGCGTG CCCAAGTTAT CCATGTCCAA CCTGGTTATG TTATCCCCTT TCCAACATGG 
GTTCCTAAAG CAGAGACAGG CCCCAGGGGT CTCACAAAAA CATTGGTGCC AAGTAAACCC 
ACAGGTGCAA CAGCTATGTC TGTTCAGGTC CAGAATTTTA CAGATGTTTG CCTCCGCTGC 
TTGGTAACTG TTCTCCTCCA GCTCTCTGAT CTGTGCCGCC TCTCGCTGTC ACTCTGTGAG 
GAGCTGGAAG AGGAGCTGCT TGTTCTGCAC AAACGTACCG CAGCCCTCCA ACACCGAACT 
GCAGCCACCT ACATAACTAT CTGTAGGGCG CGCCAGCGAG AACTAACAAC CCAGCACAGC 
AGCATCGTAC TAGCTGCTTC TGCTGTGGAC CAATCGAAGT TGGCAAACTG GCAACAACAG 
CCAGTAAATG TTTTTCTGTC CACTAAGCGG CCTCCTTGCG TGGAAGAGCT TCATCAAGAG 
GCTGAACTGA ACCTGCAGAG CCTTCTTCAG GCTGATTGCT TTACAGAAGA ATTTGAAGAT 
CGCTATCTAG ACACTAAAGT CACTGGGCAA ACATTCCGCT ATTCTGCCCT CTCGCAATCT 
GCAACACTTC CTCCAGAGAC TCCCACCAGG AATACCCCCA AGCGCCTTGA GTTTGTATAT 
CTGCCAACCA ACAGAAGAGT TAGTGAAGAT GAAACCACCA CTGCCGGTGT TAGGCCACGG 
GAGACATGCC TGAGTTTACC AACAACCCCA GACAAGCAAG TGGCTTGGAT TAAAGCTTTC 
CCATTCCCTA TCCCAGAGGA GAGGAGATGG CATCAATCTA ACTCCATCCA GGCAAACGTT 
GTACCTATCA ATGTGTCAGG GGAACTTTTT GATAAACATG CAAACTCCCG GCATTCCTTG 
TTCAATACAG AGACCGCAGT AAATCCGAAG ACCACTCTGA GACGCAGAAG GACTGTGATT 
GGATTCTCCC ACCATCAGTC ACAGGATCAC GTAGACAAAA CTCCATTGGC AGAGGCAACC 
CCCAAGCCAA GCACATCAGG CAATGTCAAC GGTGTAACGT CTATTGAACC TCCGCCAGAT 
AGAGAAGAGT CCCCACCTCC TAAGCCTGAA GCATTTCATG GGAGACATCC TGGAGGAAGA 
GCAGCTACTC CCCATCCTCA GCAGCTTCCT TTAAGAAAGA CGTTTAGTGA CCTTGGGCAT 
ATTTTGCAGT CGCCAAGCAA CATGGACAAT GCAGGTGTTA TGTATGCTAA ACCTCCATGC 
AATGGAGCGA AAGACTCTTC TATGTCTTTA AACTGGCAGG AAAGCTCATT TAACTACATG 
AGTCCATCTA GCCCAGTCAA AAGCCAGCAC AGTAGTGAAA CAACAAAACC TTCCACCTTA 
TCAATGGCTT CTCCTGGAAT GTCTAAAGGT GCAGGATCGT ATAAGCACAG AAAAGAGAAC 
ATAGTGAGGA ATAACAGTGG AAATATGTGT TCTAGTGCTA CTCTTCCAGC CCCCACAGTA 
AATCACACAA AAACTGAAGA GTTAAAAGGC ATCTTTCACA CTGGTCAAAC AGGGGGAATC 
AACTCGTCAA CATTGCCTAC ACTATCTAGT GAGTGCACTT TCCGCGAGAG GTCATTATCA 
ATGCCTACTG ACTCAGGTTC CCTATCCTCT GTAGATATTG CCTATACAGA GAACAGGAGA 
GGAAGTGGGG ACTATGCCCT AAATTATCCT AGTGCAAGCT CTGAAGAGAG CACCAGTACT 
GAGAATGTAT CTGTTTCAGC AGAGCAAGAC AACCAGGAAA GGAGGAGATC TCGGAGCATC 
TCTTTGAGAA AGCCAAAAAA GAAGCCATCA CCTCCTGCTC GCAGTGTTTC GTTGATTAAA 
GATGCAAGTG GCCAGAAATT ACAACCGGTA GCCACCATGC CAAAGGACCA ACGGCCTAAA 
AGTCTCTGTA TTGCCTTTGA TAGCCAAGGG CACAGTTATA TTCAAACATC CATGCATGGT 
AATGCAACAA TGCCAACATC AAAAAGCTTG CACAGTTGGA ACCTTAGTGA CTGCAAATCC 
AATGACTTGT ATAGGTCTCT GTCTGGCTCT AGCACAATGA CAGGTACCAC TGTGATAGAA 
CGCATGAAGG CACCAGGAAG TTCAGAATCA TTAAGTTCTC TTTCTTATTC TAGGGCCACT 
ACCCCCTCCC AGGTTTCTAC TGAGACAGAC TTAAAAATGT CTCCTGGAAA ACCTCCTGGC 
CTAATGTCAC CATCAAGTGG TTACTCTAGT CAGTCAGAGA CCCCAACTGC AGTGCCAACA 
TCATTGATTC TTGGCCACTC TCCTTACCAA AGCAGTAAGG TGAGACCACT TGTTCCTGAG 
AGAAAGTCTT CACTGCCAGC TGTTTCACCA ATTGACAGAA GCCCAAAAGT CCACCAGTCT 
TTTGATTTAC CAATTACTCC ATCTACACAT ACACATATGG ACCTAACTAC ACTGCGAAGG 
CCTTCAAAAG GCAAAATAAG GGCCAACCGA AATGCATCTG AATCAACCTT CGGTACTTCT 
AAACTTAACC AAAAGACTAG TCCAGTGCAG CCCATAATGC CTATGGTTAC CCAGTCTGAC 
TTGAGATCCA TACGTTTGCG ATCAGTCAGT AAATCAGAGC CCGACGACAG TTTTGATGGC 
ATCGATCATA TCGATGAACT TGCTGACGAA GCATTCCCGT TGCCTGAAAA AAAAATAAAA 
CCTCCAGTTG CAGAGAAACC CCCAATGTCC AGAAGACCAA TAAACCTTTC CTACAGAAAC 
CCTGCAGTTA CACCGGAATC CCCTGTTCTA TCTCCCAGTA CTCCTGGAAC ACCTGCCAGA 
GATCAAGTAT CGCCACAGGA TGCCTACCTG GTGATTGGAA CTATTAGATA CAGGAAAATG 
AATGATACAG AAGCTCGCAT AGAAACTCCC CCTTCCTTAG ACCCGTCACC CCCTTTCGAT 
AGTTGCTTTC CTGCAACCCA TTCAGTGGTT AGAAGTTTTT CGCAGAGCAG CATTGAAGAA 
GAAGAGCTCT ATAAAGGTAA ACAACTGCCT GAAAGAATAA CTCTACAAAG CTTGAACTAT 
ATGGATTCAA AGAAAATCAA GGTTCCACCG CCTATTCCCA AAAAGCCCAG TGTTTTACAT 
CTTCCAGTTT CCAGTCCACC TCCTCCTAAC CAAATGGCAT CCTCACCTTC CGAGCAACGT 
TCGTCGCCGA TCATTACTCT AGACGCTGGC ACAAGCTCTT TTCCTGACAT TATTGAGTTC 
CAAAAATATC ACGGAGTTAT CTCTGCTGCT CAGCCAGATG TAATAACTAT TGATCCTCTG 
TCAGGAGCTT TTACAGACTT ATCAGAGGAG AACAAAAGTG TATCAAACAA AACAGCAGAA 
TCAATCACAG AGGATGATGA TGAAGTTTTC GTCAGTTCAC GCACAACGGA AGACTTATTT 
ACGGTCATAC ACAGGTCAAA AAGGAAGTTA TTGGGATGGA AAGATTCTGG AGATTCTTTT 
GGAAGCCGGC AAAGCTCAGT GTCACCGGTA AAGAATTCAG GTAGCTCCAC AAGTAGTGAC 
TATTCAACTG GGACCATGAG CAGCTCGCGG TCATCCAGCA AAAATGAAGA CTTCAAAGCC 
CTTCTCCAGA GAAAAGGCAG CAAGAGCAAC ATTGGGTCTC GTCCTTCAGC TGCCGAACTT 
CTCAAGACAA CTAATCCCTT GGCACGGAGG GTCTTGAACG AGTTTGCTCC AGAGATTGAG 
AAAAGTGGCA GCACTAAAAC CTTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl2 ( XM_012968902.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824356.1 Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X2, mRNA.

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CloneID	OXa33512
Clone ID Related Accession (Same CDS sequence)	XM_012968902.2
Accession Version	XM_012968902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NHS-like protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCT ACAAGAGGAC ACTTAGGCCC CAGTCTTCCT GGGGAAGAAG TCGTCACAGT 
TCATGGTGTG TTGACACATC CCAATGTTTA ACTTCCCAGA ATGAAGGAGC CTGGGGGCAT 
CATGGACACA AACCAAGGAC TGACAATGAT AATAAAGAAG CTACCCCAGA AGAGATTGAG 
ACTGTGCGTG CCCAAGTTAT CCATGTCCAA CCTGGTTATG TTATCCCCTT TCCAACATGG 
GTTCCTAAAG CAGAGACAGG CCCCAGGGGT CTCACAAAAA CATTGGTGCC AAGTAAACCC 
ACAGGTGCAA CAGCTATGTC TGTTCAGGTC CAGAATTTTA CAGATGTTTG CCTCCGCTGC 
TTGGTAACTG TTCTCCTCCA GCTCTCTGAT CTGTGCCGCC TCTCGCTGTC ACTCTGTGAG 
GAGCTGGAAG AGGAGCTGCT TGTTCTGCAC AAACGTACCG CAGCCCTCCA ACACCGAACT 
GCAGCCACCT ACATAACTAT CTGTAGGGCG CGCCAGCGAG AACTAACAAC CCAGCACAGC 
AGCATCGTAC TAGCTGCTTC TGCTGTGGAC CAATCGAAGT TGGCAAACTG GCAACAACAG 
CCAGTAAATG TTTTTCTGTC CACTAAGCGG CCTCCTTGCG TGGAAGAGCT TCATCAAGAG 
GCTGAACTGA ACCTGCAGAG CCTTCTTCAG GCTGATTGCT TTACAGAAGA ATTTGAAGAT 
CGCTATCTAG ACACTAAAGT CACTGGGCAA ACATTCCGCT ATTCTGCCCT CTCGCAATCT 
GCAACACTTC CTCCAGAGAC TCCCACCAGG AATACCCCCA AGCGCCTTGA GTTTGTATAT 
CTGCCAACCA ACAGAAGAGT TAGTGAAGAT GAAACCACCA CTGCCGGTGT TAGGCCACGG 
GAGACATGCC TGAGTTTACC AACAACCCCA GACAAGCAAG TGGCTTGGAT TAAAGCTTTC 
CCATTCCCTA TCCCAGAGGA GAGGAGATGG CATCAATCTA ACTCCATCCA GGCAAACGTT 
GTACCTATCA ATGTGTCAGA GACCGCAGTA AATCCGAAGA CCACTCTGAG ACGCAGAAGG 
ACTGTGATTG GATTCTCCCA CCATCAGTCA CAGGATCACG TAGACAAAAC TCCATTGGCA 
GAGGCAACCC CCAAGCCAAG CACATCAGGC AATGTCAACG GTGTAACGTC TATTGAACCT 
CCGCCAGATA GAGAAGAGTC CCCACCTCCT AAGCCTGAAG CATTTCATGG GAGACATCCT 
GGAGGAAGAG CAGCTACTCC CCATCCTCAG CAGCTTCCTT TAAGAAAGAC GTTTAGTGAC 
CTTGGGCATA TTTTGCAGTC GCCAAGCAAC ATGGACAATG CAGGTGTTAT GTATGCTAAA 
CCTCCATGCA ATGGAGCGAA AGACTCTTCT ATGTCTTTAA ACTGGCAGGA AAGCTCATTT 
AACTACATGA GTCCATCTAG CCCAGTCAAA AGCCAGCACA GTAGTGAAAC AACAAAACCT 
TCCACCTTAT CAATGGCTTC TCCTGGAATG TCTAAAGGTG CAGGATCGTA TAAGCACAGA 
AAAGAGAACA TAGTGAGGAA TAACAGTGGA AATATGTGTT CTAGTGCTAC TCTTCCAGCC 
CCCACAGTAA ATCACACAAA AACTGAAGAG TTAAAAGGCA TCTTTCACAC TGGTCAAACA 
GGGGGAATCA ACTCGTCAAC ATTGCCTACA CTATCTAGTG AGTGCACTTT CCGCGAGAGG 
TCATTATCAA TGCCTACTGA CTCAGGTTCC CTATCCTCTG TAGATATTGC CTATACAGAG 
AACAGGAGAG GAAGTGGGGA CTATGCCCTA AATTATCCTA GTGCAAGCTC TGAAGAGAGC 
ACCAGTACTG AGAATGTATC TGTTTCAGCA GAGCAAGACA ACCAGGAAAG GAGGAGATCT 
CGGAGCATCT CTTTGAGAAA GCCAAAAAAG AAGCCATCAC CTCCTGCTCG CAGTGTTTCG 
TTGATTAAAG ATGCAAGTGG CCAGAAATTA CAACCGGTAG CCACCATGCC AAAGGACCAA 
CGGCCTAAAA GTCTCTGTAT TGCCTTTGAT AGCCAAGGGC ACAGTTATAT TCAAACATCC 
ATGCATGGTA ATGCAACAAT GCCAACATCA AAAAGCTTGC ACAGTTGGAA CCTTAGTGAC 
TGCAAATCCA ATGACTTGTA TAGGTCTCTG TCTGGCTCTA GCACAATGAC AGGTACCACT 
GTGATAGAAC GCATGAAGGC ACCAGGAAGT TCAGAATCAT TAAGTTCTCT TTCTTATTCT 
AGGGCCACTA CCCCCTCCCA GGTTTCTACT GAGACAGACT TAAAAATGTC TCCTGGAAAA 
CCTCCTGGCC TAATGTCACC ATCAAGTGGT TACTCTAGTC AGTCAGAGAC CCCAACTGCA 
GTGCCAACAT CATTGATTCT TGGCCACTCT CCTTACCAAA GCAGTAAGGT GAGACCACTT 
GTTCCTGAGA GAAAGTCTTC ACTGCCAGCT GTTTCACCAA TTGACAGAAG CCCAAAAGTC 
CACCAGTCTT TTGATTTACC AATTACTCCA TCTACACATA CACATATGGA CCTAACTACA 
CTGCGAAGGC CTTCAAAAGG CAAAATAAGG GCCAACCGAA ATGCATCTGA ATCAACCTTC 
GGTACTTCTA AACTTAACCA AAAGACTAGT CCAGTGCAGC CCATAATGCC TATGGTTACC 
CAGTCTGACT TGAGATCCAT ACGTTTGCGA TCAGTCAGTA AATCAGAGCC CGACGACAGT 
TTTGATGGCA TCGATCATAT CGATGAACTT GCTGACGAAG CATTCCCGTT GCCTGAAAAA 
AAAATAAAAC CTCCAGTTGC AGAGAAACCC CCAATGTCCA GAAGACCAAT AAACCTTTCC 
TACAGAAACC CTGCAGTTAC ACCGGAATCC CCTGTTCTAT CTCCCAGTAC TCCTGGAACA 
CCTGCCAGAG ATCAAGTATC GCCACAGGAT GCCTACCTGG TGATTGGAAC TATTAGATAC 
AGGAAAATGA ATGATACAGA AGCTCGCATA GAAACTCCCC CTTCCTTAGA CCCGTCACCC 
CCTTTCGATA GTTGCTTTCC TGCAACCCAT TCAGTGGTTA GAAGTTTTTC GCAGAGCAGC 
ATTGAAGAAG AAGAGCTCTA TAAAGGTAAA CAACTGCCTG AAAGAATAAC TCTACAAAGC 
TTGAACTATA TGGATTCAAA GAAAATCAAG GTTCCACCGC CTATTCCCAA AAAGCCCAGT 
GTTTTACATC TTCCAGTTTC CAGTCCACCT CCTCCTAACC AAATGGCATC CTCACCTTCC 
GAGCAACGTT CGTCGCCGAT CATTACTCTA GACGCTGGCA CAAGCTCTTT TCCTGACATT 
ATTGAGTTCC AAAAATATCA CGGAGTTATC TCTGCTGCTC AGCCAGATGT AATAACTATT 
GATCCTCTGT CAGGAGCTTT TACAGACTTA TCAGAGGAGA ACAAAAGTGT ATCAAACAAA 
ACAGCAGAAT CAATCACAGA GGATGATGAT GAAGTTTTCG TCAGTTCACG CACAACGGAA 
GACTTATTTA CGGTCATACA CAGGTCAAAA AGGAAGTTAT TGGGATGGAA AGATTCTGGA 
GATTCTTTTG GAAGCCGGCA AAGCTCAGTG TCACCGGTAA AGAATTCAGG TAGCTCCACA 
AGTAGTGACT ATTCAACTGG GACCATGAGC AGCTCGCGGT CATCCAGCAA AAATGAAGAC 
TTCAAAGCCC TTCTCCAGAG AAAAGGCAGC AAGAGCAACA TTGGGTCTCG TCCTTCAGCT 
GCCGAACTTC TCAAGACAAC TAATCCCTTG GCACGGAGGG TCTTGAACGA GTTTGCTCCA 
GAGATTGAGA AAAGTGGCAG CACTAAAACC TTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824356.1
CDS	774..4652
Translation	MPFYKRTLRPQSSWGRSRHSSWCVDTSQCLTSQNEGAWGHHGHKPRTDNDNKEATPEEIETVRAQVIHVQPGYVIPFPTWVPKAETGPRGLTKTLVPSKPTGATAMSVQVQNFTDVCLRCLVTVLLQLSDLCRLSLSLCEELEEELLVLHKRTAALQHRTAATYITICRARQRELTTQHSSIVLAASAVDQSKLANWQQQPVNVFLSTKRPPCVEELHQEAELNLQSLLQADCFTEEFEDRYLDTKVTGQTFRYSALSQSATLPPETPTRNTPKRLEFVYLPTNRRVSEDETTTAGVRPRETCLSLPTTPDKQVAWIKAFPFPIPEERRWHQSNSIQANVVPINVSETAVNPKTTLRRRRTVIGFSHHQSQDHVDKTPLAEATPKPSTSGNVNGVTSIEPPPDREESPPPKPEAFHGRHPGGRAATPHPQQLPLRKTFSDLGHILQSPSNMDNAGVMYAKPPCNGAKDSSMSLNWQESSFNYMSPSSPVKSQHSSETTKPSTLSMASPGMSKGAGSYKHRKENIVRNNSGNMCSSATLPAPTVNHTKTEELKGIFHTGQTGGINSSTLPTLSSECTFRERSLSMPTDSGSLSSVDIAYTENRRGSGDYALNYPSASSEESTSTENVSVSAEQDNQERRRSRSISLRKPKKKPSPPARSVSLIKDASGQKLQPVATMPKDQRPKSLCIAFDSQGHSYIQTSMHGNATMPTSKSLHSWNLSDCKSNDLYRSLSGSSTMTGTTVIERMKAPGSSESLSSLSYSRATTPSQVSTETDLKMSPGKPPGLMSPSSGYSSQSETPTAVPTSLILGHSPYQSSKVRPLVPERKSSLPAVSPIDRSPKVHQSFDLPITPSTHTHMDLTTLRRPSKGKIRANRNASESTFGTSKLNQKTSPVQPIMPMVTQSDLRSIRLRSVSKSEPDDSFDGIDHIDELADEAFPLPEKKIKPPVAEKPPMSRRPINLSYRNPAVTPESPVLSPSTPGTPARDQVSPQDAYLVIGTIRYRKMNDTEARIETPPSLDPSPPFDSCFPATHSVVRSFSQSSIEEEELYKGKQLPERITLQSLNYMDSKKIKVPPPIPKKPSVLHLPVSSPPPPNQMASSPSEQRSSPIITLDAGTSSFPDIIEFQKYHGVISAAQPDVITIDPLSGAFTDLSEENKSVSNKTAESITEDDDEVFVSSRTTEDLFTVIHRSKRKLLGWKDSGDSFGSRQSSVSPVKNSGSSTSSDYSTGTMSSSRSSSKNEDFKALLQRKGSKSNIGSRPSAAELLKTTNPLARRVLNEFAPEIEKSGSTKTLP

Target ORF information:

RefSeq Version	XM_012968902.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS like 2 (nhsl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968902.2

ORF Insert Sequence:

ATGCCTTTCT ACAAGAGGAC ACTTAGGCCC CAGTCTTCCT GGGGAAGAAG TCGTCACAGT 
TCATGGTGTG TTGACACATC CCAATGTTTA ACTTCCCAGA ATGAAGGAGC CTGGGGGCAT 
CATGGACACA AACCAAGGAC TGACAATGAT AATAAAGAAG CTACCCCAGA AGAGATTGAG 
ACTGTGCGTG CCCAAGTTAT CCATGTCCAA CCTGGTTATG TTATCCCCTT TCCAACATGG 
GTTCCTAAAG CAGAGACAGG CCCCAGGGGT CTCACAAAAA CATTGGTGCC AAGTAAACCC 
ACAGGTGCAA CAGCTATGTC TGTTCAGGTC CAGAATTTTA CAGATGTTTG CCTCCGCTGC 
TTGGTAACTG TTCTCCTCCA GCTCTCTGAT CTGTGCCGCC TCTCGCTGTC ACTCTGTGAG 
GAGCTGGAAG AGGAGCTGCT TGTTCTGCAC AAACGTACCG CAGCCCTCCA ACACCGAACT 
GCAGCCACCT ACATAACTAT CTGTAGGGCG CGCCAGCGAG AACTAACAAC CCAGCACAGC 
AGCATCGTAC TAGCTGCTTC TGCTGTGGAC CAATCGAAGT TGGCAAACTG GCAACAACAG 
CCAGTAAATG TTTTTCTGTC CACTAAGCGG CCTCCTTGCG TGGAAGAGCT TCATCAAGAG 
GCTGAACTGA ACCTGCAGAG CCTTCTTCAG GCTGATTGCT TTACAGAAGA ATTTGAAGAT 
CGCTATCTAG ACACTAAAGT CACTGGGCAA ACATTCCGCT ATTCTGCCCT CTCGCAATCT 
GCAACACTTC CTCCAGAGAC TCCCACCAGG AATACCCCCA AGCGCCTTGA GTTTGTATAT 
CTGCCAACCA ACAGAAGAGT TAGTGAAGAT GAAACCACCA CTGCCGGTGT TAGGCCACGG 
GAGACATGCC TGAGTTTACC AACAACCCCA GACAAGCAAG TGGCTTGGAT TAAAGCTTTC 
CCATTCCCTA TCCCAGAGGA GAGGAGATGG CATCAATCTA ACTCCATCCA GGCAAACGTT 
GTACCTATCA ATGTGTCAGA GACCGCAGTA AATCCGAAGA CCACTCTGAG ACGCAGAAGG 
ACTGTGATTG GATTCTCCCA CCATCAGTCA CAGGATCACG TAGACAAAAC TCCATTGGCA 
GAGGCAACCC CCAAGCCAAG CACATCAGGC AATGTCAACG GTGTAACGTC TATTGAACCT 
CCGCCAGATA GAGAAGAGTC CCCACCTCCT AAGCCTGAAG CATTTCATGG GAGACATCCT 
GGAGGAAGAG CAGCTACTCC CCATCCTCAG CAGCTTCCTT TAAGAAAGAC GTTTAGTGAC 
CTTGGGCATA TTTTGCAGTC GCCAAGCAAC ATGGACAATG CAGGTGTTAT GTATGCTAAA 
CCTCCATGCA ATGGAGCGAA AGACTCTTCT ATGTCTTTAA ACTGGCAGGA AAGCTCATTT 
AACTACATGA GTCCATCTAG CCCAGTCAAA AGCCAGCACA GTAGTGAAAC AACAAAACCT 
TCCACCTTAT CAATGGCTTC TCCTGGAATG TCTAAAGGTG CAGGATCGTA TAAGCACAGA 
AAAGAGAACA TAGTGAGGAA TAACAGTGGA AATATGTGTT CTAGTGCTAC TCTTCCAGCC 
CCCACAGTAA ATCACACAAA AACTGAAGAG TTAAAAGGCA TCTTTCACAC TGGTCAAACA 
GGGGGAATCA ACTCGTCAAC ATTGCCTACA CTATCTAGTG AGTGCACTTT CCGCGAGAGG 
TCATTATCAA TGCCTACTGA CTCAGGTTCC CTATCCTCTG TAGATATTGC CTATACAGAG 
AACAGGAGAG GAAGTGGGGA CTATGCCCTA AATTATCCTA GTGCAAGCTC TGAAGAGAGC 
ACCAGTACTG AGAATGTATC TGTTTCAGCA GAGCAAGACA ACCAGGAAAG GAGGAGATCT 
CGGAGCATCT CTTTGAGAAA GCCAAAAAAG AAGCCATCAC CTCCTGCTCG CAGTGTTTCG 
TTGATTAAAG ATGCAAGTGG CCAGAAATTA CAACCGGTAG CCACCATGCC AAAGGACCAA 
CGGCCTAAAA GTCTCTGTAT TGCCTTTGAT AGCCAAGGGC ACAGTTATAT TCAAACATCC 
ATGCATGGTA ATGCAACAAT GCCAACATCA AAAAGCTTGC ACAGTTGGAA CCTTAGTGAC 
TGCAAATCCA ATGACTTGTA TAGGTCTCTG TCTGGCTCTA GCACAATGAC AGGTACCACT 
GTGATAGAAC GCATGAAGGC ACCAGGAAGT TCAGAATCAT TAAGTTCTCT TTCTTATTCT 
AGGGCCACTA CCCCCTCCCA GGTTTCTACT GAGACAGACT TAAAAATGTC TCCTGGAAAA 
CCTCCTGGCC TAATGTCACC ATCAAGTGGT TACTCTAGTC AGTCAGAGAC CCCAACTGCA 
GTGCCAACAT CATTGATTCT TGGCCACTCT CCTTACCAAA GCAGTAAGGT GAGACCACTT 
GTTCCTGAGA GAAAGTCTTC ACTGCCAGCT GTTTCACCAA TTGACAGAAG CCCAAAAGTC 
CACCAGTCTT TTGATTTACC AATTACTCCA TCTACACATA CACATATGGA CCTAACTACA 
CTGCGAAGGC CTTCAAAAGG CAAAATAAGG GCCAACCGAA ATGCATCTGA ATCAACCTTC 
GGTACTTCTA AACTTAACCA AAAGACTAGT CCAGTGCAGC CCATAATGCC TATGGTTACC 
CAGTCTGACT TGAGATCCAT ACGTTTGCGA TCAGTCAGTA AATCAGAGCC CGACGACAGT 
TTTGATGGCA TCGATCATAT CGATGAACTT GCTGACGAAG CATTCCCGTT GCCTGAAAAA 
AAAATAAAAC CTCCAGTTGC AGAGAAACCC CCAATGTCCA GAAGACCAAT AAACCTTTCC 
TACAGAAACC CTGCAGTTAC ACCGGAATCC CCTGTTCTAT CTCCCAGTAC TCCTGGAACA 
CCTGCCAGAG ATCAAGTATC GCCACAGGAT GCCTACCTGG TGATTGGAAC TATTAGATAC 
AGGAAAATGA ATGATACAGA AGCTCGCATA GAAACTCCCC CTTCCTTAGA CCCGTCACCC 
CCTTTCGATA GTTGCTTTCC TGCAACCCAT TCAGTGGTTA GAAGTTTTTC GCAGAGCAGC 
ATTGAAGAAG AAGAGCTCTA TAAAGGTAAA CAACTGCCTG AAAGAATAAC TCTACAAAGC 
TTGAACTATA TGGATTCAAA GAAAATCAAG GTTCCACCGC CTATTCCCAA AAAGCCCAGT 
GTTTTACATC TTCCAGTTTC CAGTCCACCT CCTCCTAACC AAATGGCATC CTCACCTTCC 
GAGCAACGTT CGTCGCCGAT CATTACTCTA GACGCTGGCA CAAGCTCTTT TCCTGACATT 
ATTGAGTTCC AAAAATATCA CGGAGTTATC TCTGCTGCTC AGCCAGATGT AATAACTATT 
GATCCTCTGT CAGGAGCTTT TACAGACTTA TCAGAGGAGA ACAAAAGTGT ATCAAACAAA 
ACAGCAGAAT CAATCACAGA GGATGATGAT GAAGTTTTCG TCAGTTCACG CACAACGGAA 
GACTTATTTA CGGTCATACA CAGGTCAAAA AGGAAGTTAT TGGGATGGAA AGATTCTGGA 
GATTCTTTTG GAAGCCGGCA AAGCTCAGTG TCACCGGTAA AGAATTCAGG TAGCTCCACA 
AGTAGTGACT ATTCAACTGG GACCATGAGC AGCTCGCGGT CATCCAGCAA AAATGAAGAC 
TTCAAAGCCC TTCTCCAGAG AAAAGGCAGC AAGAGCAACA TTGGGTCTCG TCCTTCAGCT 
GCCGAACTTC TCAAGACAAC TAATCCCTTG GCACGGAGGG TCTTGAACGA GTTTGCTCCA 
GAGATTGAGA AAAGTGGCAG CACTAAAACC TTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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nhsl2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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