NFASC cDNA ORF clone, Bos taurus(cattle)

The following NFASC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFASC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223836 XM_024976633.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223837 XM_005216713.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223837 XM_024976634.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223838 XM_024976635.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223839 XM_024976636.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223840 XM_024976637.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223841 XM_024976638.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223842 XM_024976639.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223843 XM_024976640.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223844 XM_024976641.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223845 XM_024976642.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223846 XM_024976643.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223847 XM_024976644.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223848 XM_024976645.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223849 XM_024976646.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223850 XM_024976647.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223851 XM_024976648.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223852 XM_024976649.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223853 XM_024976650.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223854 XM_024976651.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223855 XM_024976652.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa26984 XM_005216703.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26984 XM_015466689.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26985 XM_015466690.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26985 XM_005216704.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26986 XM_015466691.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26986 XM_005216705.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26987 XM_015466692.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26987 XM_005216706.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26988 XM_005216707.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26988 XM_015466694.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26989 XM_005216708.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26989 XM_015466696.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26990 XM_005216713.2
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26990 XM_015466706.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26990 XM_015466702.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26991 XM_005216710.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26991 XM_015466697.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26992 XM_015466698.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26992 XM_002693905.4
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26993 XM_005216711.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26993 XM_015466699.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26994 XM_005216712.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26994 XM_015466700.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26995 XM_015466701.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26995 XM_003587045.4
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26996 XM_015466703.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26996 XM_002693906.4
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26997 XM_015466704.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26997 XM_005216714.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26998 XM_015466705.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa26998 XM_005216715.3
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa48428 XM_015466693.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa48428 XM_010813042.2
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa48429 XM_010813043.2
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa48429 XM_015466695.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa92881 XM_015466707.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa92881 XM_015475023.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa92882 XM_015475024.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa92882 XM_015466708.1
Latest version!
Bos taurus neurofascin (NFASC), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223836
    Clone ID Related Accession (Same CDS sequence) XM_024976633.1
    Accession Version XM_024976633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG
    AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT
    GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC
    AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC
    ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT
    GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC
    AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832401.1
    CDS101..4501
    Translation

    Target ORF information:

    RefSeq Version XM_024976633.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG
    AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT
    GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC
    AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC
    ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT
    GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC
    AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223837
    Clone ID Related Accession (Same CDS sequence) XM_005216713.3 , XM_024976634.1
    Accession Version XM_005216713.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCGC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216770.2
    CDS379..4461
    Translation

    Target ORF information:

    RefSeq Version XM_005216713.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216713.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCGC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223837
    Clone ID Related Accession (Same CDS sequence) XM_005216713.3 , XM_024976634.1
    Accession Version XM_024976634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCGC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832402.1
    CDS304..4386
    Translation

    Target ORF information:

    RefSeq Version XM_024976634.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCGC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223838
    Clone ID Related Accession (Same CDS sequence) XM_024976635.1
    Accession Version XM_024976635.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832403.1
    CDS101..4483
    Translation

    Target ORF information:

    RefSeq Version XM_024976635.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223839
    Clone ID Related Accession (Same CDS sequence) XM_024976636.1
    Accession Version XM_024976636.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC
    CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC
    AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG
    GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG
    CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC
    AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT
    GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC
    ACCTCGGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC
    GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC
    TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA
    GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA
    ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG
    GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG
    AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT
    GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG
    CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT
    GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT
    CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT
    GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG
    GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG
    GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC
    GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC
    GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC
    GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC
    ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC
    GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG
    AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG
    AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC
    GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC
    ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC
    TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC
    AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC
    GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC
    ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832404.1
    CDS101..4456
    Translation

    Target ORF information:

    RefSeq Version XM_024976636.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976636.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC
    CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC
    AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG
    GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG
    CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC
    AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT
    GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC
    ACCTCGGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC
    GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC
    TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA
    GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA
    ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG
    GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG
    AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT
    GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG
    CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT
    GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT
    CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT
    GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG
    GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG
    GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC
    GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC
    GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC
    GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC
    ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC
    GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG
    AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG
    AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC
    GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC
    ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC
    TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC
    AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC
    GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC
    ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223840
    Clone ID Related Accession (Same CDS sequence) XM_024976637.1
    Accession Version XM_024976637.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA
    GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG
    GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG
    TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG
    TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC
    GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC
    AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA
    GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC
    AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA
    AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA
    GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG
    CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC
    ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT
    ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC
    ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG
    TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC
    AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG
    ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC
    TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC
    ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG
    TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA
    CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832405.1
    CDS101..4450
    Translation

    Target ORF information:

    RefSeq Version XM_024976637.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA
    GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG
    GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG
    TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG
    TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC
    GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC
    AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA
    GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC
    AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA
    AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA
    GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG
    CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC
    ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT
    ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC
    ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG
    TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC
    AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG
    ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC
    TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC
    ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG
    TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA
    CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223841
    Clone ID Related Accession (Same CDS sequence) XM_024976638.1
    Accession Version XM_024976638.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT
    GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG
    AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG
    AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CGGCCTTCGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG
    TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA
    GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG
    CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC
    CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG
    ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC
    ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC
    GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC
    ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT
    GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC
    CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC
    TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC
    ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC
    AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC
    AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832406.1
    CDS101..4432
    Translation

    Target ORF information:

    RefSeq Version XM_024976638.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT
    GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG
    AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG
    AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CGGCCTTCGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG
    TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA
    GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG
    CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC
    CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG
    ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC
    ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC
    GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC
    ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT
    GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC
    CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC
    TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC
    ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC
    AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC
    AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223842
    Clone ID Related Accession (Same CDS sequence) XM_024976639.1
    Accession Version XM_024976639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG
    CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG
    CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA
    AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG
    ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC
    ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG
    TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC
    ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC
    CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC
    CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG
    TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832407.1
    CDS101..4273
    Translation

    Target ORF information:

    RefSeq Version XM_024976639.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG
    CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG
    CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA
    AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG
    ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC
    ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG
    TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC
    ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC
    CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC
    CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG
    TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223843
    Clone ID Related Accession (Same CDS sequence) XM_024976640.1
    Accession Version XM_024976640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC
    GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC
    GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT
    AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT
    GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG
    TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC
    GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA
    GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG
    AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832408.1
    CDS101..4258
    Translation

    Target ORF information:

    RefSeq Version XM_024976640.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC
    GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC
    GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT
    AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT
    GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG
    TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC
    GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA
    GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG
    AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223844
    Clone ID Related Accession (Same CDS sequence) XM_024976641.1
    Accession Version XM_024976641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832409.1
    CDS101..4231
    Translation

    Target ORF information:

    RefSeq Version XM_024976641.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223845
    Clone ID Related Accession (Same CDS sequence) XM_024976642.1
    Accession Version XM_024976642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC
    GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA
    ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC
    ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT
    GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC
    GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC
    CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832410.1
    CDS101..4180
    Translation

    Target ORF information:

    RefSeq Version XM_024976642.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC
    GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA
    ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC
    ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT
    GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC
    GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC
    CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223846
    Clone ID Related Accession (Same CDS sequence) XM_024976643.1
    Accession Version XM_024976643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC
    ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC
    GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA
    GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC
    TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG
    CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC
    TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG
    GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT
    TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832411.1
    CDS101..4129
    Translation

    Target ORF information:

    RefSeq Version XM_024976643.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC
    ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC
    GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA
    GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC
    TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG
    CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC
    TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG
    GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT
    TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223847
    Clone ID Related Accession (Same CDS sequence) XM_024976644.1
    Accession Version XM_024976644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832412.1
    CDS101..4003
    Translation

    Target ORF information:

    RefSeq Version XM_024976644.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223848
    Clone ID Related Accession (Same CDS sequence) XM_024976645.1
    Accession Version XM_024976645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT
    GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG
    CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG
    GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC
    GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG
    AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832413.1
    CDS101..3988
    Translation

    Target ORF information:

    RefSeq Version XM_024976645.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT
    GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG
    CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG
    GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC
    GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG
    AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223849
    Clone ID Related Accession (Same CDS sequence) XM_024976646.1
    Accession Version XM_024976646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832414.1
    CDS101..3985
    Translation

    Target ORF information:

    RefSeq Version XM_024976646.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223850
    Clone ID Related Accession (Same CDS sequence) XM_024976647.1
    Accession Version XM_024976647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCGGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832415.1
    CDS101..3940
    Translation

    Target ORF information:

    RefSeq Version XM_024976647.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCGGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223851
    Clone ID Related Accession (Same CDS sequence) XM_024976648.1
    Accession Version XM_024976648.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCGGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832416.1
    CDS101..3925
    Translation

    Target ORF information:

    RefSeq Version XM_024976648.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCGGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223852
    Clone ID Related Accession (Same CDS sequence) XM_024976649.1
    Accession Version XM_024976649.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC
    ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC
    CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT
    GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC
    AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA
    TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA
    GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCGGCC
    TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG
    AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC
    TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC
    ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC
    CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC
    CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG
    GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC
    CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT
    GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG
    CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA
    CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA
    GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA
    GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG
    GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832417.1
    CDS101..3889
    Translation

    Target ORF information:

    RefSeq Version XM_024976649.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC
    ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC
    CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT
    GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC
    AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA
    TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA
    GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCGGCC
    TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG
    AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC
    TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC
    ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC
    CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC
    CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG
    GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC
    CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT
    GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG
    CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA
    CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA
    GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA
    GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG
    GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223853
    Clone ID Related Accession (Same CDS sequence) XM_024976650.1
    Accession Version XM_024976650.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC
    CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC
    GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG
    CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC
    TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA
    GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832418.1
    CDS101..3682
    Translation

    Target ORF information:

    RefSeq Version XM_024976650.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC
    CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC
    GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG
    CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC
    TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA
    GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223854
    Clone ID Related Accession (Same CDS sequence) XM_024976651.1
    Accession Version XM_024976651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCTACAC CAACAATCAG GCAGACATTG CCACCCAGGG CTGGTTCATC
    GGACTCATGT GTGCCATCGC CCTGCTGGTG CTGATCCTGC TCATCGTCTG TTTCATCAAG
    AGGAGTCGCG GCGGCAAGTA CCCCGTGCGA GAAAAGAAGG ATGTGCCCCT CGGCCCTGAA
    GACCCCAAAG AAGAGGATGG CTCCTTTGAT TACAGTGACG AGGACAACAA GCCCCTGCAG
    GGCAGCCAGA CGTCCCTGGA CGGCACCATT AAGCAGCAGG AGAGCGACGA CAGCCTGGTG
    GACTATGGCG AGGGTGGCGA GGGCCAGTTC AACGAGGACG GCTCCTTCAT CGGCCAGTAC
    ACAGTCAAGA AGGACAAGGA GGAGACGGAA GGCAACGAGA GCTCAGAGGC CACGTCACCG
    GTCAACGCCA TCTATTCTCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832419.1
    CDS101..3667
    Translation

    Target ORF information:

    RefSeq Version XM_024976651.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCTACAC CAACAATCAG GCAGACATTG CCACCCAGGG CTGGTTCATC
    GGACTCATGT GTGCCATCGC CCTGCTGGTG CTGATCCTGC TCATCGTCTG TTTCATCAAG
    AGGAGTCGCG GCGGCAAGTA CCCCGTGCGA GAAAAGAAGG ATGTGCCCCT CGGCCCTGAA
    GACCCCAAAG AAGAGGATGG CTCCTTTGAT TACAGTGACG AGGACAACAA GCCCCTGCAG
    GGCAGCCAGA CGTCCCTGGA CGGCACCATT AAGCAGCAGG AGAGCGACGA CAGCCTGGTG
    GACTATGGCG AGGGTGGCGA GGGCCAGTTC AACGAGGACG GCTCCTTCAT CGGCCAGTAC
    ACAGTCAAGA AGGACAAGGA GGAGACGGAA GGCAACGAGA GCTCAGAGGC CACGTCACCG
    GTCAACGCCA TCTATTCTCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223855
    Clone ID Related Accession (Same CDS sequence) XM_024976652.1
    Accession Version XM_024976652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurofascin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832420.1
    CDS101..3631
    Translation

    Target ORF information:

    RefSeq Version XM_024976652.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26984
    Clone ID Related Accession (Same CDS sequence) XM_005216703.3 , XM_015466689.1
    Accession Version XM_005216703.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG
    AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT
    GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC
    AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC
    ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT
    GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC
    AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216760.1
    CDS72..4472
    Translation

    Target ORF information:

    RefSeq Version XM_005216703.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216703.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG
    AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT
    GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC
    AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC
    ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT
    GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC
    AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26984
    Clone ID Related Accession (Same CDS sequence) XM_005216703.3 , XM_015466689.1
    Accession Version XM_015466689.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG
    AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT
    GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC
    AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC
    ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT
    GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC
    AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322175.1
    CDS72..4472
    Translation

    Target ORF information:

    RefSeq Version XM_015466689.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466689.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG
    AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT
    GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC
    AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC
    ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT
    GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC
    AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26985
    Clone ID Related Accession (Same CDS sequence) XM_015466690.1 , XM_005216704.3
    Accession Version XM_015466690.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322176.1
    CDS72..4454
    Translation

    Target ORF information:

    RefSeq Version XM_015466690.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26985
    Clone ID Related Accession (Same CDS sequence) XM_015466690.1 , XM_005216704.3
    Accession Version XM_005216704.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216761.1
    CDS72..4454
    Translation

    Target ORF information:

    RefSeq Version XM_005216704.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216704.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26986
    Clone ID Related Accession (Same CDS sequence) XM_005216705.3 , XM_015466691.1
    Accession Version XM_005216705.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC
    CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC
    AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG
    GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG
    CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC
    AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT
    GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC
    ACCTCAGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC
    GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC
    TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA
    GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA
    ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG
    GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG
    AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT
    GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG
    CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT
    GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT
    CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT
    GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG
    GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG
    GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC
    GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC
    GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC
    GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC
    ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC
    GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG
    AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG
    AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC
    GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC
    ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC
    TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC
    AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC
    GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC
    ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216762.1
    CDS72..4427
    Translation

    Target ORF information:

    RefSeq Version XM_005216705.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216705.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC
    CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC
    AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG
    GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG
    CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC
    AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT
    GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC
    ACCTCAGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC
    GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC
    TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA
    GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA
    ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG
    GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG
    AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT
    GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG
    CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT
    GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT
    CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT
    GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG
    GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG
    GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC
    GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC
    GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC
    GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC
    ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC
    GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG
    AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG
    AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC
    GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC
    ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC
    TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC
    AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC
    GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC
    ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26986
    Clone ID Related Accession (Same CDS sequence) XM_005216705.3 , XM_015466691.1
    Accession Version XM_015466691.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC
    CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC
    AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG
    GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG
    CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC
    AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT
    GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC
    ACCTCAGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC
    GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC
    TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA
    GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA
    ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG
    GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG
    AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT
    GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG
    CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT
    GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT
    CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT
    GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG
    GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG
    GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC
    GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC
    GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC
    GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC
    ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC
    GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG
    AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG
    AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC
    GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC
    ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC
    TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC
    AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC
    GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC
    ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322177.1
    CDS72..4427
    Translation

    Target ORF information:

    RefSeq Version XM_015466691.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC
    CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC
    AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG
    GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG
    CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC
    AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT
    GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC
    ACCTCAGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC
    GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC
    TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA
    GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA
    ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG
    GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG
    AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT
    GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG
    CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT
    GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT
    CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT
    GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG
    GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG
    GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC
    GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC
    GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC
    GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC
    ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC
    GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG
    AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG
    AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC
    GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC
    ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC
    TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC
    AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC
    GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC
    ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26987
    Clone ID Related Accession (Same CDS sequence) XM_015466692.1 , XM_005216706.3
    Accession Version XM_015466692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA
    GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG
    GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG
    TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG
    TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC
    GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC
    AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA
    GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC
    AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA
    AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA
    GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG
    CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC
    ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT
    ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC
    ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG
    TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC
    AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG
    ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC
    TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC
    ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG
    TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA
    CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322178.1
    CDS72..4421
    Translation

    Target ORF information:

    RefSeq Version XM_015466692.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA
    GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG
    GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG
    TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG
    TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC
    GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC
    AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA
    GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC
    AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA
    AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA
    GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG
    CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC
    ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT
    ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC
    ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG
    TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC
    AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG
    ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC
    TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC
    ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG
    TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA
    CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26987
    Clone ID Related Accession (Same CDS sequence) XM_015466692.1 , XM_005216706.3
    Accession Version XM_005216706.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA
    GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG
    GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG
    TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG
    TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC
    GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC
    AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA
    GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC
    AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA
    AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA
    GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG
    CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC
    ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT
    ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC
    ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG
    TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC
    AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG
    ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC
    TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC
    ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG
    TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA
    CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216763.1
    CDS72..4421
    Translation

    Target ORF information:

    RefSeq Version XM_005216706.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216706.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA
    GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG
    GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG
    TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG
    TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC
    GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC
    AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA
    GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC
    AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA
    AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA
    GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG
    CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC
    ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT
    ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC
    ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG
    TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC
    AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG
    ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC
    TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC
    ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG
    TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA
    CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26988
    Clone ID Related Accession (Same CDS sequence) XM_005216707.3 , XM_015466694.1
    Accession Version XM_005216707.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG
    CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG
    CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA
    AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG
    ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC
    ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG
    TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC
    ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC
    CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC
    CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG
    TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216764.1
    CDS72..4244
    Translation

    Target ORF information:

    RefSeq Version XM_005216707.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216707.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG
    CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG
    CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA
    AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG
    ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC
    ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG
    TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC
    ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC
    CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC
    CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG
    TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26988
    Clone ID Related Accession (Same CDS sequence) XM_005216707.3 , XM_015466694.1
    Accession Version XM_015466694.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG
    CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG
    CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA
    AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG
    ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC
    ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG
    TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC
    ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC
    CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC
    CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG
    TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322180.1
    CDS72..4244
    Translation

    Target ORF information:

    RefSeq Version XM_015466694.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466694.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG
    CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG
    CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA
    AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG
    ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC
    ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG
    TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC
    ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC
    CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC
    CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG
    TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26989
    Clone ID Related Accession (Same CDS sequence) XM_005216708.3 , XM_015466696.1
    Accession Version XM_005216708.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216765.1
    CDS72..4202
    Translation

    Target ORF information:

    RefSeq Version XM_005216708.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216708.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26989
    Clone ID Related Accession (Same CDS sequence) XM_005216708.3 , XM_015466696.1
    Accession Version XM_015466696.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322182.1
    CDS72..4202
    Translation

    Target ORF information:

    RefSeq Version XM_015466696.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466696.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG
    TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC
    GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC
    GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT
    CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26990
    Clone ID Related Accession (Same CDS sequence) XM_005216713.2 , XM_015466702.1 , XM_015466706.1
    Accession Version XM_005216713.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005216713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216770.1
    CDS361..4443
    Translation

    Target ORF information:

    RefSeq Version XM_005216713.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216713.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26990
    Clone ID Related Accession (Same CDS sequence) XM_005216713.2 , XM_015466702.1 , XM_015466706.1
    Accession Version XM_015466702.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322188.1
    CDS361..4443
    Translation

    Target ORF information:

    RefSeq Version XM_015466702.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466702.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26990
    Clone ID Related Accession (Same CDS sequence) XM_005216713.2 , XM_015466702.1 , XM_015466706.1
    Accession Version XM_015466706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322192.1
    CDS296..4378
    Translation

    Target ORF information:

    RefSeq Version XM_015466706.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
    GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG
    GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC
    ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA
    GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG
    ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26991
    Clone ID Related Accession (Same CDS sequence) XM_005216710.3 , XM_015466697.1
    Accession Version XM_005216710.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC
    GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA
    ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC
    ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT
    GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC
    GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC
    CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216767.1
    CDS72..4151
    Translation

    Target ORF information:

    RefSeq Version XM_005216710.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216710.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC
    GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA
    ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC
    ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT
    GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC
    GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC
    CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26991
    Clone ID Related Accession (Same CDS sequence) XM_005216710.3 , XM_015466697.1
    Accession Version XM_015466697.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC
    GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA
    ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC
    ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT
    GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC
    GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC
    CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322183.1
    CDS72..4151
    Translation

    Target ORF information:

    RefSeq Version XM_015466697.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466697.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC
    GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA
    ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC
    ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT
    GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC
    GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC
    CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26992
    Clone ID Related Accession (Same CDS sequence) XM_002693905.4 , XM_015466698.1
    Accession Version XM_002693905.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002693905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC
    ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC
    GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA
    GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC
    TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG
    CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC
    TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG
    GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT
    TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002693951.2
    CDS72..4100
    Translation

    Target ORF information:

    RefSeq Version XM_002693905.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002693905.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC
    ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC
    GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA
    GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC
    TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG
    CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC
    TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG
    GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT
    TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26992
    Clone ID Related Accession (Same CDS sequence) XM_002693905.4 , XM_015466698.1
    Accession Version XM_015466698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC
    ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC
    GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA
    GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC
    TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG
    CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC
    TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG
    GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT
    TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322184.1
    CDS72..4100
    Translation

    Target ORF information:

    RefSeq Version XM_015466698.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC
    ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC
    GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA
    GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC
    TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG
    CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC
    TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG
    GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT
    TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26993
    Clone ID Related Accession (Same CDS sequence) XM_005216711.3 , XM_015466699.1
    Accession Version XM_005216711.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216768.1
    CDS72..3974
    Translation

    Target ORF information:

    RefSeq Version XM_005216711.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216711.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26993
    Clone ID Related Accession (Same CDS sequence) XM_005216711.3 , XM_015466699.1
    Accession Version XM_015466699.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322185.1
    CDS72..3974
    Translation

    Target ORF information:

    RefSeq Version XM_015466699.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466699.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC
    AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG
    CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC
    GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC
    TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC
    ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG
    ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26994
    Clone ID Related Accession (Same CDS sequence) XM_015466700.1 , XM_005216712.3
    Accession Version XM_015466700.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT
    GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG
    CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG
    GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC
    GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG
    AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322186.1
    CDS72..3959
    Translation

    Target ORF information:

    RefSeq Version XM_015466700.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466700.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT
    GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG
    CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG
    GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC
    GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG
    AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26994
    Clone ID Related Accession (Same CDS sequence) XM_015466700.1 , XM_005216712.3
    Accession Version XM_005216712.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT
    GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG
    CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG
    GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC
    GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG
    AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216769.1
    CDS72..3959
    Translation

    Target ORF information:

    RefSeq Version XM_005216712.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216712.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT
    GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG
    CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG
    GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC
    GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG
    AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26995
    Clone ID Related Accession (Same CDS sequence) XM_003587045.4 , XM_015466701.1
    Accession Version XM_003587045.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587093.2
    CDS72..3956
    Translation

    Target ORF information:

    RefSeq Version XM_003587045.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587045.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26995
    Clone ID Related Accession (Same CDS sequence) XM_003587045.4 , XM_015466701.1
    Accession Version XM_015466701.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322187.1
    CDS72..3956
    Translation

    Target ORF information:

    RefSeq Version XM_015466701.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466701.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC
    AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG
    AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT
    GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG
    GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC
    CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT
    CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT
    GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA
    TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA
    GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26996
    Clone ID Related Accession (Same CDS sequence) XM_015466703.1 , XM_002693906.4
    Accession Version XM_015466703.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322189.1
    CDS72..3911
    Translation

    Target ORF information:

    RefSeq Version XM_015466703.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466703.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26996
    Clone ID Related Accession (Same CDS sequence) XM_015466703.1 , XM_002693906.4
    Accession Version XM_002693906.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002693906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002693952.2
    CDS72..3911
    Translation

    Target ORF information:

    RefSeq Version XM_002693906.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002693906.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT
    CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG
    GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG
    CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT
    GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC
    ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG
    GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26997
    Clone ID Related Accession (Same CDS sequence) XM_015466704.1 , XM_005216714.3
    Accession Version XM_015466704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322190.1
    CDS72..3896
    Translation

    Target ORF information:

    RefSeq Version XM_015466704.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26997
    Clone ID Related Accession (Same CDS sequence) XM_015466704.1 , XM_005216714.3
    Accession Version XM_005216714.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216771.1
    CDS72..3896
    Translation

    Target ORF information:

    RefSeq Version XM_005216714.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216714.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC
    CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT
    GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC
    AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT
    ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC
    AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC
    AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC
    GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG
    TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC
    AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC
    AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC
    TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC
    GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC
    TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG
    CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA
    GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG
    GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA
    GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG
    TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC
    AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC
    AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC
    ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC
    TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC
    ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA
    GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC
    AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA
    GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT
    GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC
    ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC
    ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT
    GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC
    TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC
    TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26998
    Clone ID Related Accession (Same CDS sequence) XM_005216715.3 , XM_015466705.1
    Accession Version XM_005216715.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC
    ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC
    CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT
    GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC
    AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA
    TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA
    GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCAGCC
    TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG
    AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC
    TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC
    ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC
    CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC
    CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG
    GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC
    CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT
    GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG
    CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA
    CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA
    GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA
    GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG
    GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216772.1
    CDS72..3860
    Translation

    Target ORF information:

    RefSeq Version XM_005216715.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216715.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC
    ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC
    CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT
    GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC
    AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA
    TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA
    GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCAGCC
    TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG
    AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC
    TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC
    ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC
    CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC
    CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG
    GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC
    CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT
    GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG
    CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA
    CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA
    GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA
    GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG
    GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26998
    Clone ID Related Accession (Same CDS sequence) XM_005216715.3 , XM_015466705.1
    Accession Version XM_015466705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC
    ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC
    CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT
    GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC
    AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA
    TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA
    GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCAGCC
    TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG
    AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC
    TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC
    ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC
    CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC
    CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG
    GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC
    CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT
    GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG
    CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA
    CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA
    GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA
    GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG
    GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322191.1
    CDS72..3860
    Translation

    Target ORF information:

    RefSeq Version XM_015466705.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC
    ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC
    CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT
    GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC
    AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA
    TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA
    GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCAGCC
    TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG
    AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC
    TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC
    ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC
    CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC
    CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG
    GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC
    CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT
    GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG
    CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA
    CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA
    GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA
    GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG
    GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC
    ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC
    GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG
    TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT
    GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG
    GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG
    GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48428
    Clone ID Related Accession (Same CDS sequence) XM_015466693.1 , XM_010813042.2
    Accession Version XM_015466693.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT
    GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG
    AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG
    AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CAGCCTTCGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG
    TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA
    GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG
    CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC
    CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG
    ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC
    ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC
    GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC
    ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT
    GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC
    CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC
    TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC
    ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC
    AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC
    AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322179.1
    CDS72..4403
    Translation

    Target ORF information:

    RefSeq Version XM_015466693.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT
    GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG
    AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG
    AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CAGCCTTCGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG
    TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA
    GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG
    CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC
    CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG
    ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC
    ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC
    GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC
    ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT
    GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC
    CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC
    TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC
    ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC
    AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC
    AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48428
    Clone ID Related Accession (Same CDS sequence) XM_015466693.1 , XM_010813042.2
    Accession Version XM_010813042.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT
    GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG
    AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG
    AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CAGCCTTCGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG
    TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA
    GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG
    CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC
    CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG
    ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC
    ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC
    GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC
    ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT
    GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC
    CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC
    TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC
    ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC
    AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC
    AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811344.1
    CDS72..4403
    Translation

    Target ORF information:

    RefSeq Version XM_010813042.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813042.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG
    ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT
    GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC
    TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC
    GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC
    AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG
    TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG
    TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT
    GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC
    ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG
    AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC
    ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC
    AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG
    CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT
    GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG
    CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC
    AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG
    GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG
    CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT
    GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT
    GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC
    ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC
    GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC
    GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT
    GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG
    AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG
    AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CAGCCTTCGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG
    TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA
    GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG
    CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC
    CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG
    ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC
    ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC
    GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC
    ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT
    GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC
    CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC
    TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC
    ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC
    AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC
    AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48429
    Clone ID Related Accession (Same CDS sequence) XM_010813043.2 , XM_015466695.1
    Accession Version XM_010813043.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC
    GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC
    GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT
    AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT
    GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG
    TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC
    GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA
    GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG
    AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811345.1
    CDS72..4229
    Translation

    Target ORF information:

    RefSeq Version XM_010813043.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813043.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC
    GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC
    GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT
    AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT
    GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG
    TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC
    GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA
    GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG
    AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48429
    Clone ID Related Accession (Same CDS sequence) XM_010813043.2 , XM_015466695.1
    Accession Version XM_015466695.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC
    GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC
    GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT
    AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT
    GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG
    TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC
    GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA
    GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG
    AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322181.1
    CDS72..4229
    Translation

    Target ORF information:

    RefSeq Version XM_015466695.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC
    AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC
    AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC
    AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA
    GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC
    GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC
    GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT
    AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT
    GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG
    TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC
    GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA
    GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG
    AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92881
    Clone ID Related Accession (Same CDS sequence) XM_015475023.1 , XM_015466707.1
    Accession Version XM_015475023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC
    CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC
    GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG
    CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC
    TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA
    GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330509.1
    CDS72..3653
    Translation

    Target ORF information:

    RefSeq Version XM_015475023.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC
    CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC
    GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG
    CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC
    TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA
    GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92881
    Clone ID Related Accession (Same CDS sequence) XM_015475023.1 , XM_015466707.1
    Accession Version XM_015466707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC
    CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC
    GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG
    CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC
    TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA
    GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322193.1
    CDS72..3653
    Translation

    Target ORF information:

    RefSeq Version XM_015466707.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA
    GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG
    CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT
    GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC
    AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT
    CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA
    TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG
    CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG
    CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC
    ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC
    CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC
    TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC
    AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG
    AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC
    ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC
    CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC
    CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG
    ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT
    GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG
    GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT
    ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC
    CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC
    GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG
    CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC
    TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA
    GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92882
    Clone ID Related Accession (Same CDS sequence) XM_015475024.1 , XM_015466708.1
    Accession Version XM_015475024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330510.1
    CDS72..3602
    Translation

    Target ORF information:

    RefSeq Version XM_015475024.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92882
    Clone ID Related Accession (Same CDS sequence) XM_015475024.1 , XM_015466708.1
    Accession Version XM_015466708.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurofascin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322194.1
    CDS72..3602
    Translation

    Target ORF information:

    RefSeq Version XM_015466708.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466708.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
    CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG
    TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG
    GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC
    CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG
    CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC
    CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT
    GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC
    CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG
    ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG
    TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA
    TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG
    GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT
    GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG
    GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC
    CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC
    ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT
    CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA
    CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG
    AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT
    GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA
    CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA
    CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC
    CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC
    TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC
    CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT
    GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT
    GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC
    CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC
    CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG
    GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC
    TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT
    TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC
    ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT
    CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG
    CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC
    AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC
    GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG
    GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA
    GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC
    TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT
    GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG
    CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG
    ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC
    ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG
    AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG
    CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG
    GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC
    GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.