NFASC cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFASC
Entrez Gene ID	23114
Full Name	neurofascin
Synonyms	NF,NRCAML
General protein information	Preferred Names neurofascin Names neurofascin neurofascin homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009].
Disorder MIM:	609145

mRNA and Protein(s)

mRNA	Protein	Name
XM_011509313.1	XP_011507615.1	neurofascin isoform X3
XM_011509323.1	XP_011507625.1	neurofascin isoform X16
XM_011509322.1	XP_011507624.1	neurofascin isoform X15
XM_011509321.1	XP_011507623.1	neurofascin isoform X13
XM_011509327.1	XP_011507629.1	neurofascin isoform X28
XM_011509311.2	XP_011507613.2	neurofascin isoform X2
XM_011509312.1	XP_011507614.1	neurofascin isoform X3
XM_011509323.2	XP_011507625.2	neurofascin isoform X16
XM_005244993.3	XP_005245050.2	neurofascin isoform X24
XM_024454301.1	XP_024310069.1	neurofascin isoform X31
XM_017000741.1	XP_016856230.1	neurofascin isoform X32
XM_011509328.2	XP_011507630.2	neurofascin isoform X36
NM_001005388.2	NP_001005388.2	neurofascin isoform 1 precursor
XM_011509325.1	XP_011507627.1	neurofascin isoform X18
XM_017000736.1	XP_016856225.1	neurofascin isoform X20
XM_011509315.1	XP_011507617.1	neurofascin isoform X6
XM_017000737.1	XP_016856226.1	neurofascin isoform X24
NM_001365986.1	NP_001352915.1	neurofascin isoform 7 precursor
NM_001160333.2	NP_001153805.1	neurofascin isoform 6 precursor
XM_024454288.1	XP_024310056.1	neurofascin isoform X5
XM_024454292.1	XP_024310060.1	neurofascin isoform X8
XM_011509321.2	XP_011507623.2	neurofascin isoform X14
XM_011509322.2	XP_011507624.2	neurofascin isoform X15
XM_011509325.2	XP_011507627.2	neurofascin isoform X19
XM_005244991.3	XP_005245048.2	neurofascin isoform X22
XM_005244992.4	XP_005245049.2	neurofascin isoform X23
XM_011509327.2	XP_011507629.2	neurofascin isoform X29
NM_001005389.1	NP_001005389.2	neurofascin isoform 5 precursor
XM_011509311.1	XP_011507613.1	neurofascin isoform X1
XM_011509317.1	XP_011507619.1	neurofascin isoform X9
NM_001160332.1	NP_001153804.1	neurofascin isoform 3 precursor
XM_011509316.1	XP_011507618.1	neurofascin isoform X10
XM_011509319.1	XP_011507621.1	neurofascin isoform X12
XM_024454296.1	XP_024310064.1	neurofascin isoform X17
XM_024454297.1	XP_024310065.1	neurofascin isoform X18
XM_005244989.3	XP_005245046.2	neurofascin isoform X20
XM_017000739.1	XP_016856228.1	neurofascin isoform X26
XM_011509326.2	XP_011507628.2	neurofascin isoform X28
XM_024454302.1	XP_024310070.1	neurofascin isoform X31
XM_017000743.1	XP_016856232.1	neurofascin isoform X35
XM_017000735.1	XP_016856224.1	neurofascin isoform X14
XM_011509328.1	XP_011507630.1	neurofascin isoform X34
NM_001160333.1	NP_001153805.1	neurofascin isoform 6 precursor
NM_001160331.1	NP_001153803.1	neurofascin isoform 2 precursor
NM_001005389.2	NP_001005389.2	neurofascin isoform 5 precursor
NM_015090.3	NP_055905.2	neurofascin isoform 4 precursor
XM_024454283.1	XP_024310051.1	neurofascin isoform X1
XM_017000734.1	XP_016856223.1	neurofascin isoform X9
XM_011509318.2	XP_011507620.1	neurofascin isoform X11
XM_017000738.1	XP_016856227.1	neurofascin isoform X25
XM_017000742.2	XP_016856231.1	neurofascin isoform X33
XM_011509326.1	XP_011507628.1	neurofascin isoform X27
XM_017000742.1	XP_016856231.1	neurofascin isoform X31
XM_011509324.1	XP_011507626.1	neurofascin isoform X17
XM_024454285.1	XP_024310053.1	neurofascin isoform X4
XM_017000733.1	XP_016856222.1	neurofascin isoform X6
XM_011509314.1	XP_011507616.1	neurofascin isoform X7
XM_011509320.2	XP_011507622.1	neurofascin isoform X13
XM_024454299.1	XP_024310067.1	neurofascin isoform X21
XM_024454300.1	XP_024310068.1	neurofascin isoform X27
XM_017000740.1	XP_016856229.1	neurofascin isoform X30
XM_005244997.3	XP_005245054.2	neurofascin isoform X34

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nfasc	NP_874385.1
Xenopus tropicalis (tropical clawed frog)	nfasc	XP_004910755.1
Rattus norvegicus (Norway rat)	Nfasc	NP_001153786.1
Danio rerio (zebrafish)	nfasca	NP_001139239.1
	NFASC	XP_001096628.1
Canis lupus familiaris (dog)	NFASC	XP_005640834.1
Pan troglodytes (chimpanzee)	NFASC	XP_003308755.1
Gallus gallus (chicken)	NFASC	NP_001153794.1
Homo sapiens (human)	NFASC	NP_001005388.2
Bos taurus (cattle)	NFASC	XP_005216764.1

Gene Ontology
Bibliography

Related articles in PubMed

Copy number variation, increased gene expression, and molecular mechanisms of neurofascin in lung cancer.
Samulin Erdem J, Arnoldussen YJ, Skaug V, Haugen A, Zienolddiny S
Molecular carcinogenesis56(9)2076-2085(2017 Sep)

Autoantibodies to nodal isoforms of neurofascin in chronic inflammatory demyelinating polyneuropathy.
Delmont E, Manso C, Querol L, Cortese A, Berardinelli A, Lozza A, Belghazi M, Malissart P, Labauge P, Taieb G, Yuki N, Illa I, Attarian S, Devaux JJ
Brain : a journal of neurology140(7)1851-1858(2017 Jul)

Neurofascin-155 IgG4 in chronic inflammatory demyelinating polyneuropathy.
Devaux JJ, Miura Y, Fukami Y, Inoue T, Manso C, Belghazi M, Sekiguchi K, Kokubun N, Ichikawa H, Wong AH, Yuki N
Neurology86(9)800-7(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggest that NFASC is a novel regulator of non-small cell lung cancer cell motility and support a role of NFASC in the regulation of non-small cell lung cancer progression
Title: Copy number variation, increased gene expression, and molecular mechanisms of neurofascin in lung cancer.

identified neurofascin as the target of the autoantibodies in Chronic inflammatory demyelination polyneuropathy patients.
Title: Autoantibodies to nodal isoforms of neurofascin in chronic inflammatory demyelinating polyneuropathy.

-NF155 IgG4 antibodies were associated with a subgroup of patients with CIDP showing a younger age at onset, ataxia, tremor, CNS demyelination, and a poor response to IV immunoglobulin.
Title: Neurofascin-155 IgG4 in chronic inflammatory demyelinating polyneuropathy.

Autoantibodies to NF155 identify a inflammatory demyelinating polyradiculoneuropathy subtype characterized by severe neuropathy, poor response to intravenous immunoglobulin, and disabling tremor.
Title: Neurofascin IgG4 antibodies in CIDP associate with disabling tremor and poor response to IVIg.

It is a common protein to the central and peripheral nervous system may play a pivotal role in combined demyelination in Combined central and peripheral demyelination.
Title: [Neurofascin: a novel target for combined central and peripheral demyelination].

The following NFASC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFASC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00703	NM_001160331.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$599.50-$839.30 $1199.00
OHu23142	NM_001005388.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu23142	XM_024454300.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26373	NM_001005389.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
OHu27075	NM_001160333.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
OHu65479	XM_005244997.3 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu104326	XM_024454301.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu104326	XM_024454302.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu87059	XM_017000741.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu87060	XM_017000742.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu87061	XM_017000743.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu65458	XM_011509311.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu65459	XM_011509312.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu65460	XM_011509313.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu65461	XM_011509314.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu65462	XM_011509315.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu65463	XM_011509316.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu65464	XM_011509317.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu65465	XM_011509318.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu65466	XM_011509319.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu65467	XM_011509320.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu65469	XM_011509322.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu65470	XM_011509323.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu107102	NM_001365986.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu104314	XM_024454285.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104315	XM_024454288.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104316	XM_024454292.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104317	XM_011509321.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104318	XM_011509322.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104319	XM_011509323.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104320	XM_024454296.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104321	XM_024454297.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104322	XM_011509325.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104323	XM_024454299.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104324	XM_011509326.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104325	XM_011509327.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104327	XM_011509328.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu87051	XM_017000733.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu87052	XM_017000734.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu87053	XM_017000735.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu87054	XM_017000736.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu87055	XM_017000737.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu87056	XM_017000738.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu87057	XM_017000739.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu87058	XM_017000740.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu01378	NM_001160332.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$832.30 $1189.00
OHu02001	NM_015090.3 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu65468	XM_011509321.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65471	XM_011509324.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65472	XM_011509325.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65473	XM_005244989.3 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65474	XM_005244991.3 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65475	XM_005244992.4 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65476	XM_005244993.3 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65477	XM_011509326.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65478	XM_011509327.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65480	XM_011509328.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu104312	XM_024454283.1 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu104313	XM_011509311.2 Latest version!	Homo sapiens neurofascin (NFASC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OHu27075	NM_001160333.1	Homo sapiens neurofascin (NFASC), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
OHu26373	NM_001005389.1	Homo sapiens neurofascin (NFASC), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
OHu87060	XM_017000742.1	Homo sapiens neurofascin (NFASC), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFASC ( NM_001160331.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153803.1 Homo sapiens neurofascin (NFASC), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00703
Clone ID Related Accession (Same CDS sequence)	NM_001160331.1
Accession Version	NM_001160331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	neurofascin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL391822.12 and BC035389.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The promoter and 5' UTR for this variant have not been determined. The resulting isoform (2) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences near the N-terminus, compared to isoform 1. Isoform 2 is also known as NF155, as described by Davis et al. (PMID: 8947556). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCAAC CACCCTTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC 
CGGCCCCGGG ACCTGGAGCT GACCGACCTG GCCGAGAGGA GCGTGCGGCT GACCTGGATC 
CCCGGGGATG CTAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAAGACCAG 
TTCCAACCTG GGGTCTGGCA TGACCATTCC AAGTACCCCG GCAGCGTTAA CTCAGCCGTC 
CTCCGGCTGT CCCCGTATGT CAACTACCAG TTCCGTGTCA TTGCCATCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTCCC ATCCGAGCGC TACCGAACCA GTGGAGCACC CCCCGAGTCC 
AATCCTGGTG ACGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCCATGAATG CCACCTCGGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGAGGCGG 
AGAGAGACTC GAGAGGCCTG GAACAACGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACCCCAG TCTACGTGCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTCGGGAAG 
GGCCCTGAGC CAGAGTCCGT CATCGGTTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAGTCCGAGT CATGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTACTCCAA CCGCAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153803.1
CDS	91..3660
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160331.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160331.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCAAC CACCCTTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC 
CGGCCCCGGG ACCTGGAGCT GACCGACCTG GCCGAGAGGA GCGTGCGGCT GACCTGGATC 
CCCGGGGATG CTAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAAGACCAG 
TTCCAACCTG GGGTCTGGCA TGACCATTCC AAGTACCCCG GCAGCGTTAA CTCAGCCGTC 
CTCCGGCTGT CCCCGTATGT CAACTACCAG TTCCGTGTCA TTGCCATCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTCCC ATCCGAGCGC TACCGAACCA GTGGAGCACC CCCCGAGTCC 
AATCCTGGTG ACGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCCATGAATG CCACCTCGGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGAGGCGG 
AGAGAGACTC GAGAGGCCTG GAACAACGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACCCCAG TCTACGTGCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTCGGGAAG 
GGCCCTGAGC CAGAGTCCGT CATCGGTTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAGTCCGAGT CATGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTACTCCAA CCGCAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001005388.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005388.2 Homo sapiens neurofascin (NFASC), transcript variant 1, mRNA.

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CloneID	OHu23142
Clone ID Related Accession (Same CDS sequence)	NM_001005388.2 , XM_024454300.1
Accession Version	NM_001005388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	neurofascin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC040674.1, BC117674.2, AB018299.3, AL391822.12 and BC035389.1. This sequence is a reference standard in the RefSeqGene project. On May 21, 2009 this sequence version replaced NM_001005388.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform lacks the third fibronectin type 3 (FNIII) repeat and includes the mucin-like domain. Isoform 1 is also known as NF186, as described by Davis et al. (PMID: 8947556). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.189883.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTGCGACG 
GGCGCTGTGA GCAGTACCGA TGCTACTGCC ATTGCTGCCA CCACCGAAGC CACAACAGTC 
CCCATCATCC CAACTGTCGC ACCTACCACC ATCGCCACCA CCACCACCGT CGCCACAACT 
ACTACAACCA CTGCTGCCGC CACCACCACC ACGGAGAGTC CTCCCACCAC CACCTCCGGG 
ACTAAGATAC ACGAATCCGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005388.2
CDS	329..4051
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001005388.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005388.2

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTGCGACG 
GGCGCTGTGA GCAGTACCGA TGCTACTGCC ATTGCTGCCA CCACCGAAGC CACAACAGTC 
CCCATCATCC CAACTGTCGC ACCTACCACC ATCGCCACCA CCACCACCGT CGCCACAACT 
ACTACAACCA CTGCTGCCGC CACCACCACC ACGGAGAGTC CTCCCACCAC CACCTCCGGG 
ACTAAGATAC ACGAATCCGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454300.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310068.1 Homo sapiens neurofascin (NFASC), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OHu23142
Clone ID Related Accession (Same CDS sequence)	NM_001005388.2 , XM_024454300.1
Accession Version	XM_024454300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTGCGACG 
GGCGCTGTGA GCAGTACCGA TGCTACTGCC ATTGCTGCCA CCACCGAAGC CACAACAGTC 
CCCATCATCC CAACTGTCGC ACCTACCACC ATCGCCACCA CCACCACCGT CGCCACAACT 
ACTACAACCA CTGCTGCCGC CACCACCACC ACGGAGAGTC CTCCCACCAC CACCTCCGGG 
ACTAAGATAC ACGAATCCGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310068.1
CDS	188..3910
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024454300.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454300.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTGCGACG 
GGCGCTGTGA GCAGTACCGA TGCTACTGCC ATTGCTGCCA CCACCGAAGC CACAACAGTC 
CCCATCATCC CAACTGTCGC ACCTACCACC ATCGCCACCA CCACCACCGT CGCCACAACT 
ACTACAACCA CTGCTGCCGC CACCACCACC ACGGAGAGTC CTCCCACCAC CACCTCCGGG 
ACTAAGATAC ACGAATCCGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001005389.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005389.2 Homo sapiens neurofascin (NFASC), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu26373
Clone ID Related Accession (Same CDS sequence)	NM_001005389.1 , NM_001005389.2
Accession Version	NM_001005389.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-22
Organism	Homo sapiens(human)
Product	neurofascin isoform 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC040674.1, AK090639.1 and AA514945.1. Transcript Variant: This variant (5) uses an alternate 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (5) has a substantially shorter and distinct C-terminus that lacks the fibronectin domains, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK090639.1, SRR1803612.157606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GGTAACTGCC CATGCTCACC CTGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005389.2
CDS	329..2188
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCPCSPWH

Target ORF information:

RefSeq Version	NM_001005389.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005389.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GGTAACTGCC CATGCTCACC CTGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001005389.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005389.2 Homo sapiens neurofascin (NFASC), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu26373
Clone ID Related Accession (Same CDS sequence)	NM_001005389.1 , NM_001005389.2
Accession Version	NM_001005389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	neurofascin isoform 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC096675.2, BC040674.1, AK090639.1 and AA514945.1. On Jun 2, 2019 this sequence version replaced NM_001005389.1. Transcript Variant: This variant (5) uses an alternate 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (5) has a substantially shorter and distinct C-terminus that lacks the fibronectin domains, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK090639.1, SRR1803612.157606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GGTAACTGCC CATGCTCACC CTGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005389.2
CDS	331..2190
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCPCSPWH

Target ORF information:

RefSeq Version	NM_001005389.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005389.2

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GGTAACTGCC CATGCTCACC CTGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001160333.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153805.1 Homo sapiens neurofascin (NFASC), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu27075
Clone ID Related Accession (Same CDS sequence)	NM_001160333.2 , NM_001160333.1
Accession Version	NM_001160333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	neurofascin isoform 6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC096675.2, BC040674.1, AK299262.1, AK090639.1 and AA514945.1. On Jun 2, 2019 this sequence version replaced NM_001160333.1. Transcript Variant: This variant (6) lacks the second coding exon and uses an alternate 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (5) lacks an internal domain and has a substantially shorter and distinct C-terminus that lacks the fibronectin domains, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.125827.1, SRR1803611.148860.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC ACCAAGCTTC 
ATGTATCCCC AGGGCACCGC GAGCAGCCAG ATGGTGCTTC GTGGCATGGA CCTCCTGCTG 
GAATGCATCG CCTCCGGGGT CCCAACACCA GACATCGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTG ATAAGGCCAA GTTTGAGAAC TTTAATAAGG CCCTGCGTAT CACAAATGTC 
TCTGAGGAAG ACTCCGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT 
ATTCTGGCTC CTGGCGAGGA TGGGAGACTG GTGTGTCGAG CCAATGGAAA CCCCAAACCC 
ACTGTCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCTAA CCCAAACCGT 
GAGGTGGCCG GAGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG GGCTGTGTAC 
CAGTGCAACA CCTCCAACGA GCATGGCTAC CTGCTGGCCA ACGCCTTTGT CAGTGTGCTG 
GATGTGCCGC CTCGGATGCT GTCGCCCCGG AACCAGCTCA TTCGAGTGAT TCTTTACAAC 
CGGACGCGGC TGGACTGCCC TTTCTTTGGG TCTCCCATCC CCACACTGCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGC AACTACCATG TTTATGAGAA CGGCAGTCTG 
GAAATTAAGA TGATCCGCAA AGAGGACCAG GGCATCTACA CCTGTGTCGC CACCAACATC 
CTGGGCAAAG CTGAAAACCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG 
ATGCCCGAGG ACCAGGTGGC CAGAAGGGGC ACCACGGTGC AGCTGGAGTG TCGGGTGAAG 
CACGACCCCT CCCTGAAACT CACCGTCTCC TGGCTGAAGG ATGACGAGCC GCTCTATATT 
GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGGGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTGT CGCCAGCACC GAGCTAGACC AAGACCTGGC CAAGGCCTAC 
CTCACCGTGC TAGGTAACTG CCCATGCTCA CCCTGGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153805.1
CDS	331..2172
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCPCSPWH

Target ORF information:

RefSeq Version	NM_001160333.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160333.2

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC ACCAAGCTTC 
ATGTATCCCC AGGGCACCGC GAGCAGCCAG ATGGTGCTTC GTGGCATGGA CCTCCTGCTG 
GAATGCATCG CCTCCGGGGT CCCAACACCA GACATCGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTG ATAAGGCCAA GTTTGAGAAC TTTAATAAGG CCCTGCGTAT CACAAATGTC 
TCTGAGGAAG ACTCCGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT 
ATTCTGGCTC CTGGCGAGGA TGGGAGACTG GTGTGTCGAG CCAATGGAAA CCCCAAACCC 
ACTGTCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCTAA CCCAAACCGT 
GAGGTGGCCG GAGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG GGCTGTGTAC 
CAGTGCAACA CCTCCAACGA GCATGGCTAC CTGCTGGCCA ACGCCTTTGT CAGTGTGCTG 
GATGTGCCGC CTCGGATGCT GTCGCCCCGG AACCAGCTCA TTCGAGTGAT TCTTTACAAC 
CGGACGCGGC TGGACTGCCC TTTCTTTGGG TCTCCCATCC CCACACTGCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGC AACTACCATG TTTATGAGAA CGGCAGTCTG 
GAAATTAAGA TGATCCGCAA AGAGGACCAG GGCATCTACA CCTGTGTCGC CACCAACATC 
CTGGGCAAAG CTGAAAACCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG 
ATGCCCGAGG ACCAGGTGGC CAGAAGGGGC ACCACGGTGC AGCTGGAGTG TCGGGTGAAG 
CACGACCCCT CCCTGAAACT CACCGTCTCC TGGCTGAAGG ATGACGAGCC GCTCTATATT 
GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGGGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTGT CGCCAGCACC GAGCTAGACC AAGACCTGGC CAAGGCCTAC 
CTCACCGTGC TAGGTAACTG CCCATGCTCA CCCTGGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001160333.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153805.1 Homo sapiens neurofascin (NFASC), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu27075
Clone ID Related Accession (Same CDS sequence)	NM_001160333.2 , NM_001160333.1
Accession Version	NM_001160333.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-22
Organism	Homo sapiens(human)
Product	neurofascin isoform 6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC040674.1, AK299262.1, AK090639.1 and AA514945.1. Transcript Variant: This variant (6) lacks the second coding exon and uses an alternate 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (5) lacks an internal domain and has a substantially shorter and distinct C-terminus that lacks the fibronectin domains, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.148860.1, SRR1660803.125827.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC ACCAAGCTTC 
ATGTATCCCC AGGGCACCGC GAGCAGCCAG ATGGTGCTTC GTGGCATGGA CCTCCTGCTG 
GAATGCATCG CCTCCGGGGT CCCAACACCA GACATCGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTG ATAAGGCCAA GTTTGAGAAC TTTAATAAGG CCCTGCGTAT CACAAATGTC 
TCTGAGGAAG ACTCCGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT 
ATTCTGGCTC CTGGCGAGGA TGGGAGACTG GTGTGTCGAG CCAATGGAAA CCCCAAACCC 
ACTGTCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCTAA CCCAAACCGT 
GAGGTGGCCG GAGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG GGCTGTGTAC 
CAGTGCAACA CCTCCAACGA GCATGGCTAC CTGCTGGCCA ACGCCTTTGT CAGTGTGCTG 
GATGTGCCGC CTCGGATGCT GTCGCCCCGG AACCAGCTCA TTCGAGTGAT TCTTTACAAC 
CGGACGCGGC TGGACTGCCC TTTCTTTGGG TCTCCCATCC CCACACTGCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGC AACTACCATG TTTATGAGAA CGGCAGTCTG 
GAAATTAAGA TGATCCGCAA AGAGGACCAG GGCATCTACA CCTGTGTCGC CACCAACATC 
CTGGGCAAAG CTGAAAACCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG 
ATGCCCGAGG ACCAGGTGGC CAGAAGGGGC ACCACGGTGC AGCTGGAGTG TCGGGTGAAG 
CACGACCCCT CCCTGAAACT CACCGTCTCC TGGCTGAAGG ATGACGAGCC GCTCTATATT 
GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGGGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTGT CGCCAGCACC GAGCTAGACC AAGACCTGGC CAAGGCCTAC 
CTCACCGTGC TAGGTAACTG CCCATGCTCA CCCTGGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153805.1
CDS	329..2170
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCPCSPWH

Target ORF information:

RefSeq Version	NM_001160333.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160333.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC ACCAAGCTTC 
ATGTATCCCC AGGGCACCGC GAGCAGCCAG ATGGTGCTTC GTGGCATGGA CCTCCTGCTG 
GAATGCATCG CCTCCGGGGT CCCAACACCA GACATCGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTG ATAAGGCCAA GTTTGAGAAC TTTAATAAGG CCCTGCGTAT CACAAATGTC 
TCTGAGGAAG ACTCCGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT 
ATTCTGGCTC CTGGCGAGGA TGGGAGACTG GTGTGTCGAG CCAATGGAAA CCCCAAACCC 
ACTGTCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCTAA CCCAAACCGT 
GAGGTGGCCG GAGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG GGCTGTGTAC 
CAGTGCAACA CCTCCAACGA GCATGGCTAC CTGCTGGCCA ACGCCTTTGT CAGTGTGCTG 
GATGTGCCGC CTCGGATGCT GTCGCCCCGG AACCAGCTCA TTCGAGTGAT TCTTTACAAC 
CGGACGCGGC TGGACTGCCC TTTCTTTGGG TCTCCCATCC CCACACTGCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGC AACTACCATG TTTATGAGAA CGGCAGTCTG 
GAAATTAAGA TGATCCGCAA AGAGGACCAG GGCATCTACA CCTGTGTCGC CACCAACATC 
CTGGGCAAAG CTGAAAACCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG 
ATGCCCGAGG ACCAGGTGGC CAGAAGGGGC ACCACGGTGC AGCTGGAGTG TCGGGTGAAG 
CACGACCCCT CCCTGAAACT CACCGTCTCC TGGCTGAAGG ATGACGAGCC GCTCTATATT 
GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGGGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTGT CGCCAGCACC GAGCTAGACC AAGACCTGGC CAAGGCCTAC 
CTCACCGTGC TAGGTAACTG CCCATGCTCA CCCTGGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005244997.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245054.2 Homo sapiens neurofascin (NFASC), transcript variant X35, mRNA.

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CloneID	OHu65479
Clone ID Related Accession (Same CDS sequence)	XM_005244997.3
Accession Version	XM_005244997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005244997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGTAACT GCCCATGCTC ACCCTGGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245054.2
CDS	178..2250
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCPCSPWH

Target ORF information:

RefSeq Version	XM_005244997.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005244997.3

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGTAACT GCCCATGCTC ACCCTGGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454301.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310069.1 Homo sapiens neurofascin (NFASC), transcript variant X31, mRNA.

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CloneID	OHu104326
Clone ID Related Accession (Same CDS sequence)	XM_024454301.1 , XM_024454302.1
Accession Version	XM_024454301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT ATCCCAGGGC TGCGCCCACT GAAGTTAAAG TCCGAGTCAT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGCGTCTAC TCCGACACGG TCCAGGGCCA GCTCAGAGAG 
TACCGAGTGA GGAAGCCAGC TCCGGACTCA CCTAACTACC CAGCTCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310069.1
CDS	288..2918
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRVRKPAPDSPNYPAH

Target ORF information:

RefSeq Version	XM_024454301.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454301.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT ATCCCAGGGC TGCGCCCACT GAAGTTAAAG TCCGAGTCAT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGCGTCTAC TCCGACACGG TCCAGGGCCA GCTCAGAGAG 
TACCGAGTGA GGAAGCCAGC TCCGGACTCA CCTAACTACC CAGCTCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454302.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310070.1 Homo sapiens neurofascin (NFASC), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OHu104326
Clone ID Related Accession (Same CDS sequence)	XM_024454301.1 , XM_024454302.1
Accession Version	XM_024454302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT ATCCCAGGGC TGCGCCCACT GAAGTTAAAG TCCGAGTCAT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGCGTCTAC TCCGACACGG TCCAGGGCCA GCTCAGAGAG 
TACCGAGTGA GGAAGCCAGC TCCGGACTCA CCTAACTACC CAGCTCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310070.1
CDS	384..3014
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRVRKPAPDSPNYPAH

Target ORF information:

RefSeq Version	XM_024454302.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454302.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT ATCCCAGGGC TGCGCCCACT GAAGTTAAAG TCCGAGTCAT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGCGTCTAC TCCGACACGG TCCAGGGCCA GCTCAGAGAG 
TACCGAGTGA GGAAGCCAGC TCCGGACTCA CCTAACTACC CAGCTCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000741.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856230.1 Homo sapiens neurofascin (NFASC), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OHu87059
Clone ID Related Accession (Same CDS sequence)	XM_017000741.1
Accession Version	XM_017000741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG GGGAACCTTT GCAATCGGCA CCACCTAACC CAAACCGTGA GGTGGCCGGA 
GACACCATCA TCTTCCGGGA CACCCAGATC AGCAGCAGGG CTGTGTACCA GTGCAACACC 
TCCAACGAGC ATGGCTACCT GCTGGCCAAC GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT 
CGGATGCTGT CGCCCCGGAA CCAGCTCATT CGAGTGATTC TTTACAACCG GACGCGGCTG 
GACTGCCCTT TCTTTGGGTC TCCCATCCCC ACACTGCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGTGGCAA CTACCATGTT TATGAGAACG GCAGTCTGGA AATTAAGATG 
ATCCGCAAAG AGGACCAGGG CATCTACACC TGTGTCGCCA CCAACATCCT GGGCAAAGCT 
GAAAACCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACCGGAT GCCCGAGGAC 
CAGGTGGCCA GAAGGGGCAC CACGGTGCAG CTGGAGTGTC GGGTGAAGCA CGACCCCTCC 
CTGAAACTCA CCGTCTCCTG GCTGAAGGAT GACGAGCCGC TCTATATTGG AAACAGGATG 
AAGAAGGAAG ACGACTCCCT GACCATCTTT GGGGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTGTCG CCAGCACCGA GCTAGACCAA GACCTGGCCA AGGCCTACCT CACCGTGCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTGC CCAAAGGACG GCCAGACCGG 
CCCCGGGACC TGGAGCTGAC CGACCTGGCC GAGAGGAGCG TGCGGCTGAC CTGGATCCCC 
GGGGATGCTA ACAACAGCCC CATCACAGAC TACGTCGTCC AGTTTGAAGA AGACCAGTTC 
CAACCTGGGG TCTGGCATGA CCATTCCAAG TACCCCGGCA GCGTTAACTC AGCCGTCCTC 
CGGCTGTCCC CGTATGTCAA CTACCAGTTC CGTGTCATTG CCATCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTCCCATC CGAGCGCTAC CGAACCAGTG GAGCACCCCC CGAGTCCAAT 
CCTGGTGACG TGAAGGGAGA GGGGACCAGA AAGAACAACA TGGAGATCAC GTGGACGCCC 
ATGAATGCCA CCTCGGCCTT TGGCCCCAAC CTGCGCTACA TTGTCAAGTG GAGGCGGAGA 
GAGACTCGAG AGGCCTGGAA CAACGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACCCCAGTCT ACGTGCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT CGGGAAGGGC 
CCTGAGCCAG AGTCCGTCAT CGGTTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAG TCCGAGTCAT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGCGTCTAC 
TCCGACACGG TCCAGGGCCA GCTCAGAGAG TACCGAGCCT ACTACTGGAG GGAGAGCAGC 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAAG CCAGCTTCCC TGGTGACCGC 
CTCCGTGGCG TGGTGTCCCG CCTCTTCCCC TACAGTAACT ACAAGCTGGA GATGGTTGTG 
GTCAATGGGA GAGGTGATGG GCCTCGCAGT GAGACCAAGG AGTTCACCAC CCCGGAAGGA 
GTACCCAGTG CCCCTAGGCG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG 
GAATGGGATC ATCCTGAGCA TCCAAATGGG ATCATGATTG GATACACTCT CAAATATGTG 
GCCTTTAACG GGACCAAAGT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCACGG TGCAAAGAAC GGACCCCGTG TCACGCTACC GCTTTACCCT CAGCGCCAGG 
ACGCAGGTGG GCTCTGGGGA AGCCGTCACA GAGGAGTCAC CAGCACCCCC GAATGAAGCT 
ACTCCAACCG CAGCTCCTCC CACATTGCCC CCGACTACCG TGGGTGCGAC GGGCGCTGTG 
AGCAGTACCG ATGCTACTGC CATTGCTGCC ACCACCGAAG CCACAACAGT CCCCATCATC 
CCAACTGTCG CACCTACCAC CATCGCCACC ACCACCACCG TCGCCACAAC TACTACAACC 
ACTGCTGCCG CCACCACCAC CACGGAGAGT CCTCCCACCA CCACCTCCGG GACTAAGATA 
CACGAATCCG CCCCTGATGA GCAGTCCATA TGGAACGTCA CGGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC GGGCCCGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCAGTTAAGG CCCAGGCTCA GCCTATACAG 
CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGGG TTTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA CAACCAAGCG 
GACATCGCCA CCCAGGGCTG GTTCATTGGG CTTATGTGCG CCATCGCCCT CCTGGTGCTG 
ATCCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGCGGCG GCAAGTACCC AGTACGAGAA 
AAGAAGGATG TTCCCCTTGG CCCTGAAGAC CCCAAGGAAG AGGATGGCTC ATTTGACTAT 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGTCAGACAT CTCTGGACGG CACCATCAAG 
CAGCAGGAGA GTGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG TCAGTTCAAT 
GAAGACGGCT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAGGAGGA AACAGAGGGC 
AACGAAAGCT CAGAGGCCAC GTCACCTGTC AATGCTATCT ACTCTCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856230.1
CDS	1042..3975
Translation	MVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000741.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000741.1

ORF Insert Sequence:

ATGGTGAATG GGGAACCTTT GCAATCGGCA CCACCTAACC CAAACCGTGA GGTGGCCGGA 
GACACCATCA TCTTCCGGGA CACCCAGATC AGCAGCAGGG CTGTGTACCA GTGCAACACC 
TCCAACGAGC ATGGCTACCT GCTGGCCAAC GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT 
CGGATGCTGT CGCCCCGGAA CCAGCTCATT CGAGTGATTC TTTACAACCG GACGCGGCTG 
GACTGCCCTT TCTTTGGGTC TCCCATCCCC ACACTGCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGTGGCAA CTACCATGTT TATGAGAACG GCAGTCTGGA AATTAAGATG 
ATCCGCAAAG AGGACCAGGG CATCTACACC TGTGTCGCCA CCAACATCCT GGGCAAAGCT 
GAAAACCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACCGGAT GCCCGAGGAC 
CAGGTGGCCA GAAGGGGCAC CACGGTGCAG CTGGAGTGTC GGGTGAAGCA CGACCCCTCC 
CTGAAACTCA CCGTCTCCTG GCTGAAGGAT GACGAGCCGC TCTATATTGG AAACAGGATG 
AAGAAGGAAG ACGACTCCCT GACCATCTTT GGGGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTGTCG CCAGCACCGA GCTAGACCAA GACCTGGCCA AGGCCTACCT CACCGTGCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTGC CCAAAGGACG GCCAGACCGG 
CCCCGGGACC TGGAGCTGAC CGACCTGGCC GAGAGGAGCG TGCGGCTGAC CTGGATCCCC 
GGGGATGCTA ACAACAGCCC CATCACAGAC TACGTCGTCC AGTTTGAAGA AGACCAGTTC 
CAACCTGGGG TCTGGCATGA CCATTCCAAG TACCCCGGCA GCGTTAACTC AGCCGTCCTC 
CGGCTGTCCC CGTATGTCAA CTACCAGTTC CGTGTCATTG CCATCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTCCCATC CGAGCGCTAC CGAACCAGTG GAGCACCCCC CGAGTCCAAT 
CCTGGTGACG TGAAGGGAGA GGGGACCAGA AAGAACAACA TGGAGATCAC GTGGACGCCC 
ATGAATGCCA CCTCGGCCTT TGGCCCCAAC CTGCGCTACA TTGTCAAGTG GAGGCGGAGA 
GAGACTCGAG AGGCCTGGAA CAACGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACCCCAGTCT ACGTGCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT CGGGAAGGGC 
CCTGAGCCAG AGTCCGTCAT CGGTTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAG TCCGAGTCAT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGCGTCTAC 
TCCGACACGG TCCAGGGCCA GCTCAGAGAG TACCGAGCCT ACTACTGGAG GGAGAGCAGC 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAAG CCAGCTTCCC TGGTGACCGC 
CTCCGTGGCG TGGTGTCCCG CCTCTTCCCC TACAGTAACT ACAAGCTGGA GATGGTTGTG 
GTCAATGGGA GAGGTGATGG GCCTCGCAGT GAGACCAAGG AGTTCACCAC CCCGGAAGGA 
GTACCCAGTG CCCCTAGGCG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG 
GAATGGGATC ATCCTGAGCA TCCAAATGGG ATCATGATTG GATACACTCT CAAATATGTG 
GCCTTTAACG GGACCAAAGT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCACGG TGCAAAGAAC GGACCCCGTG TCACGCTACC GCTTTACCCT CAGCGCCAGG 
ACGCAGGTGG GCTCTGGGGA AGCCGTCACA GAGGAGTCAC CAGCACCCCC GAATGAAGCT 
ACTCCAACCG CAGCTCCTCC CACATTGCCC CCGACTACCG TGGGTGCGAC GGGCGCTGTG 
AGCAGTACCG ATGCTACTGC CATTGCTGCC ACCACCGAAG CCACAACAGT CCCCATCATC 
CCAACTGTCG CACCTACCAC CATCGCCACC ACCACCACCG TCGCCACAAC TACTACAACC 
ACTGCTGCCG CCACCACCAC CACGGAGAGT CCTCCCACCA CCACCTCCGG GACTAAGATA 
CACGAATCCG CCCCTGATGA GCAGTCCATA TGGAACGTCA CGGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC GGGCCCGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCAGTTAAGG CCCAGGCTCA GCCTATACAG 
CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGGG TTTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA CAACCAAGCG 
GACATCGCCA CCCAGGGCTG GTTCATTGGG CTTATGTGCG CCATCGCCCT CCTGGTGCTG 
ATCCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGCGGCG GCAAGTACCC AGTACGAGAA 
AAGAAGGATG TTCCCCTTGG CCCTGAAGAC CCCAAGGAAG AGGATGGCTC ATTTGACTAT 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGTCAGACAT CTCTGGACGG CACCATCAAG 
CAGCAGGAGA GTGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG TCAGTTCAAT 
GAAGACGGCT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAGGAGGA AACAGAGGGC 
AACGAAAGCT CAGAGGCCAC GTCACCTGTC AATGCTATCT ACTCTCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000742.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856231.1 Homo sapiens neurofascin (NFASC), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OHu87060
Clone ID Related Accession (Same CDS sequence)	XM_017000742.2 , XM_017000742.1
Accession Version	XM_017000742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG GGGAACCTTT GCAATCGGCA CCACCTAACC CAAACCGTGA GGTGGCCGGA 
GACACCATCA TCTTCCGGGA CACCCAGATC AGCAGCAGGG CTGTGTACCA GTGCAACACC 
TCCAACGAGC ATGGCTACCT GCTGGCCAAC GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT 
CGGATGCTGT CGCCCCGGAA CCAGCTCATT CGAGTGATTC TTTACAACCG GACGCGGCTG 
GACTGCCCTT TCTTTGGGTC TCCCATCCCC ACACTGCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGTGGCAA CTACCATGTT TATGAGAACG GCAGTCTGGA AATTAAGATG 
ATCCGCAAAG AGGACCAGGG CATCTACACC TGTGTCGCCA CCAACATCCT GGGCAAAGCT 
GAAAACCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACCGGAT GCCCGAGGAC 
CAGGTGGCCA GAAGGGGCAC CACGGTGCAG CTGGAGTGTC GGGTGAAGCA CGACCCCTCC 
CTGAAACTCA CCGTCTCCTG GCTGAAGGAT GACGAGCCGC TCTATATTGG AAACAGGATG 
AAGAAGGAAG ACGACTCCCT GACCATCTTT GGGGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTGTCG CCAGCACCGA GCTAGACCAA GACCTGGCCA AGGCCTACCT CACCGTGCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTGC CCAAAGGACG GCCAGACCGG 
CCCCGGGACC TGGAGCTGAC CGACCTGGCC GAGAGGAGCG TGCGGCTGAC CTGGATCCCC 
GGGGATGCTA ACAACAGCCC CATCACAGAC TACGTCGTCC AGTTTGAAGA AGACCAGTTC 
CAACCTGGGG TCTGGCATGA CCATTCCAAG TACCCCGGCA GCGTTAACTC AGCCGTCCTC 
CGGCTGTCCC CGTATGTCAA CTACCAGTTC CGTGTCATTG CCATCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTCCCATC CGAGCGCTAC CGAACCAGTG GAGCACCCCC CGAGTCCAAT 
CCTGGTGACG TGAAGGGAGA GGGGACCAGA AAGAACAACA TGGAGATCAC GTGGACGCCC 
ATGAATGCCA CCTCGGCCTT TGGCCCCAAC CTGCGCTACA TTGTCAAGTG GAGGCGGAGA 
GAGACTCGAG AGGCCTGGAA CAACGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACCCCAGTCT ACGTGCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT CGGGAAGGGC 
CCTGAGCCAG AGTCCGTCAT CGGTTACTCC GGAGAAGATT TACCCAGTGC CCCTAGGCGT 
TTCCGAGTCC GGCAGCCCAA CCTGGAGACA ATCAACCTGG AATGGGATCA TCCTGAGCAT 
CCAAATGGGA TCATGATTGG ATACACTCTC AAATATGTGG CCTTTAACGG GACCAAAGTA 
GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC AATCAGACCA AGTTCACGGT GCAAAGAACG 
GACCCCGTGT CACGCTACCG CTTTACCCTC AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA 
GCCGTCACAG AGGAGTCACC AGCACCCCCG AATGAAGCTA CTCCAACCGC AGCTCCTCCC 
ACATTGCCCC CGACTACCGT GGGTGCGACG GGCGCTGTGA GCAGTACCGA TGCTACTGCC 
ATTGCTGCCA CCACCGAAGC CACAACAGTC CCCATCATCC CAACTGTCGC ACCTACCACC 
ATCGCCACCA CCACCACCGT CGCCACAACT ACTACAACCA CTGCTGCCGC CACCACCACC 
ACGGAGAGTC CTCCCACCAC CACCTCCGGG ACTAAGATAC ACGAATCCGC CCCTGATGAG 
CAGTCCATAT GGAACGTCAC GGTGCTCCCC AACAGTAAAT GGGCCAACAT CACCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA TCGACAGCAA CCATACGAAA 
AAAACTGTCC CAGTTAAGGC CCAGGCTCAG CCTATACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGGGT TTATTCCCGG GACAACGAGG GCATCAGCAG TACCGTCATC 
ACCTTTATGA CCAGTACAGC TTACACCAAC AACCAAGCGG ACATCGCCAC CCAGGGCTGG 
TTCATTGGGC TTATGTGCGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCA GTACGAGAAA AGAAGGATGT TCCCCTTGGC 
CCTGAAGACC CCAAGGAAGA GGATGGCTCA TTTGACTATA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GTCAGACATC TCTGGACGGC ACCATCAAGC AGCAGGAGAG TGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGT CAGTTCAATG AAGACGGCTC CTTCATCGGC 
CAGTACACGG TCAAAAAGGA CAAGGAGGAA ACAGAGGGCA ACGAAAGCTC AGAGGCCACG 
TCACCTGTCA ATGCTATCTA CTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856231.1
CDS	1097..3709
Translation	MVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000742.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000742.2

ORF Insert Sequence:

ATGGTGAATG GGGAACCTTT GCAATCGGCA CCACCTAACC CAAACCGTGA GGTGGCCGGA 
GACACCATCA TCTTCCGGGA CACCCAGATC AGCAGCAGGG CTGTGTACCA GTGCAACACC 
TCCAACGAGC ATGGCTACCT GCTGGCCAAC GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT 
CGGATGCTGT CGCCCCGGAA CCAGCTCATT CGAGTGATTC TTTACAACCG GACGCGGCTG 
GACTGCCCTT TCTTTGGGTC TCCCATCCCC ACACTGCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGTGGCAA CTACCATGTT TATGAGAACG GCAGTCTGGA AATTAAGATG 
ATCCGCAAAG AGGACCAGGG CATCTACACC TGTGTCGCCA CCAACATCCT GGGCAAAGCT 
GAAAACCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACCGGAT GCCCGAGGAC 
CAGGTGGCCA GAAGGGGCAC CACGGTGCAG CTGGAGTGTC GGGTGAAGCA CGACCCCTCC 
CTGAAACTCA CCGTCTCCTG GCTGAAGGAT GACGAGCCGC TCTATATTGG AAACAGGATG 
AAGAAGGAAG ACGACTCCCT GACCATCTTT GGGGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTGTCG CCAGCACCGA GCTAGACCAA GACCTGGCCA AGGCCTACCT CACCGTGCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTGC CCAAAGGACG GCCAGACCGG 
CCCCGGGACC TGGAGCTGAC CGACCTGGCC GAGAGGAGCG TGCGGCTGAC CTGGATCCCC 
GGGGATGCTA ACAACAGCCC CATCACAGAC TACGTCGTCC AGTTTGAAGA AGACCAGTTC 
CAACCTGGGG TCTGGCATGA CCATTCCAAG TACCCCGGCA GCGTTAACTC AGCCGTCCTC 
CGGCTGTCCC CGTATGTCAA CTACCAGTTC CGTGTCATTG CCATCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTCCCATC CGAGCGCTAC CGAACCAGTG GAGCACCCCC CGAGTCCAAT 
CCTGGTGACG TGAAGGGAGA GGGGACCAGA AAGAACAACA TGGAGATCAC GTGGACGCCC 
ATGAATGCCA CCTCGGCCTT TGGCCCCAAC CTGCGCTACA TTGTCAAGTG GAGGCGGAGA 
GAGACTCGAG AGGCCTGGAA CAACGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACCCCAGTCT ACGTGCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT CGGGAAGGGC 
CCTGAGCCAG AGTCCGTCAT CGGTTACTCC GGAGAAGATT TACCCAGTGC CCCTAGGCGT 
TTCCGAGTCC GGCAGCCCAA CCTGGAGACA ATCAACCTGG AATGGGATCA TCCTGAGCAT 
CCAAATGGGA TCATGATTGG ATACACTCTC AAATATGTGG CCTTTAACGG GACCAAAGTA 
GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC AATCAGACCA AGTTCACGGT GCAAAGAACG 
GACCCCGTGT CACGCTACCG CTTTACCCTC AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA 
GCCGTCACAG AGGAGTCACC AGCACCCCCG AATGAAGCTA CTCCAACCGC AGCTCCTCCC 
ACATTGCCCC CGACTACCGT GGGTGCGACG GGCGCTGTGA GCAGTACCGA TGCTACTGCC 
ATTGCTGCCA CCACCGAAGC CACAACAGTC CCCATCATCC CAACTGTCGC ACCTACCACC 
ATCGCCACCA CCACCACCGT CGCCACAACT ACTACAACCA CTGCTGCCGC CACCACCACC 
ACGGAGAGTC CTCCCACCAC CACCTCCGGG ACTAAGATAC ACGAATCCGC CCCTGATGAG 
CAGTCCATAT GGAACGTCAC GGTGCTCCCC AACAGTAAAT GGGCCAACAT CACCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA TCGACAGCAA CCATACGAAA 
AAAACTGTCC CAGTTAAGGC CCAGGCTCAG CCTATACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGGGT TTATTCCCGG GACAACGAGG GCATCAGCAG TACCGTCATC 
ACCTTTATGA CCAGTACAGC TTACACCAAC AACCAAGCGG ACATCGCCAC CCAGGGCTGG 
TTCATTGGGC TTATGTGCGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCA GTACGAGAAA AGAAGGATGT TCCCCTTGGC 
CCTGAAGACC CCAAGGAAGA GGATGGCTCA TTTGACTATA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GTCAGACATC TCTGGACGGC ACCATCAAGC AGCAGGAGAG TGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGT CAGTTCAATG AAGACGGCTC CTTCATCGGC 
CAGTACACGG TCAAAAAGGA CAAGGAGGAA ACAGAGGGCA ACGAAAGCTC AGAGGCCACG 
TCACCTGTCA ATGCTATCTA CTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000742.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856231.1 Homo sapiens neurofascin (NFASC), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OHu87060
Clone ID Related Accession (Same CDS sequence)	XM_017000742.2 , XM_017000742.1
Accession Version	XM_017000742.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG GGGAACCTTT GCAATCGGCA CCACCTAACC CAAACCGTGA GGTGGCCGGA 
GACACCATCA TCTTCCGGGA CACCCAGATC AGCAGCAGGG CTGTGTACCA GTGCAACACC 
TCCAACGAGC ATGGCTACCT GCTGGCCAAC GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT 
CGGATGCTGT CGCCCCGGAA CCAGCTCATT CGAGTGATTC TTTACAACCG GACGCGGCTG 
GACTGCCCTT TCTTTGGGTC TCCCATCCCC ACACTGCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGTGGCAA CTACCATGTT TATGAGAACG GCAGTCTGGA AATTAAGATG 
ATCCGCAAAG AGGACCAGGG CATCTACACC TGTGTCGCCA CCAACATCCT GGGCAAAGCT 
GAAAACCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACCGGAT GCCCGAGGAC 
CAGGTGGCCA GAAGGGGCAC CACGGTGCAG CTGGAGTGTC GGGTGAAGCA CGACCCCTCC 
CTGAAACTCA CCGTCTCCTG GCTGAAGGAT GACGAGCCGC TCTATATTGG AAACAGGATG 
AAGAAGGAAG ACGACTCCCT GACCATCTTT GGGGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTGTCG CCAGCACCGA GCTAGACCAA GACCTGGCCA AGGCCTACCT CACCGTGCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTGC CCAAAGGACG GCCAGACCGG 
CCCCGGGACC TGGAGCTGAC CGACCTGGCC GAGAGGAGCG TGCGGCTGAC CTGGATCCCC 
GGGGATGCTA ACAACAGCCC CATCACAGAC TACGTCGTCC AGTTTGAAGA AGACCAGTTC 
CAACCTGGGG TCTGGCATGA CCATTCCAAG TACCCCGGCA GCGTTAACTC AGCCGTCCTC 
CGGCTGTCCC CGTATGTCAA CTACCAGTTC CGTGTCATTG CCATCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTCCCATC CGAGCGCTAC CGAACCAGTG GAGCACCCCC CGAGTCCAAT 
CCTGGTGACG TGAAGGGAGA GGGGACCAGA AAGAACAACA TGGAGATCAC GTGGACGCCC 
ATGAATGCCA CCTCGGCCTT TGGCCCCAAC CTGCGCTACA TTGTCAAGTG GAGGCGGAGA 
GAGACTCGAG AGGCCTGGAA CAACGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACCCCAGTCT ACGTGCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT CGGGAAGGGC 
CCTGAGCCAG AGTCCGTCAT CGGTTACTCC GGAGAAGATT TACCCAGTGC CCCTAGGCGT 
TTCCGAGTCC GGCAGCCCAA CCTGGAGACA ATCAACCTGG AATGGGATCA TCCTGAGCAT 
CCAAATGGGA TCATGATTGG ATACACTCTC AAATATGTGG CCTTTAACGG GACCAAAGTA 
GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC AATCAGACCA AGTTCACGGT GCAAAGAACG 
GACCCCGTGT CACGCTACCG CTTTACCCTC AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA 
GCCGTCACAG AGGAGTCACC AGCACCCCCG AATGAAGCTA CTCCAACCGC AGCTCCTCCC 
ACATTGCCCC CGACTACCGT GGGTGCGACG GGCGCTGTGA GCAGTACCGA TGCTACTGCC 
ATTGCTGCCA CCACCGAAGC CACAACAGTC CCCATCATCC CAACTGTCGC ACCTACCACC 
ATCGCCACCA CCACCACCGT CGCCACAACT ACTACAACCA CTGCTGCCGC CACCACCACC 
ACGGAGAGTC CTCCCACCAC CACCTCCGGG ACTAAGATAC ACGAATCCGC CCCTGATGAG 
CAGTCCATAT GGAACGTCAC GGTGCTCCCC AACAGTAAAT GGGCCAACAT CACCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA TCGACAGCAA CCATACGAAA 
AAAACTGTCC CAGTTAAGGC CCAGGCTCAG CCTATACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGGGT TTATTCCCGG GACAACGAGG GCATCAGCAG TACCGTCATC 
ACCTTTATGA CCAGTACAGC TTACACCAAC AACCAAGCGG ACATCGCCAC CCAGGGCTGG 
TTCATTGGGC TTATGTGCGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCA GTACGAGAAA AGAAGGATGT TCCCCTTGGC 
CCTGAAGACC CCAAGGAAGA GGATGGCTCA TTTGACTATA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GTCAGACATC TCTGGACGGC ACCATCAAGC AGCAGGAGAG TGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGT CAGTTCAATG AAGACGGCTC CTTCATCGGC 
CAGTACACGG TCAAAAAGGA CAAGGAGGAA ACAGAGGGCA ACGAAAGCTC AGAGGCCACG 
TCACCTGTCA ATGCTATCTA CTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856231.1
CDS	1188..3800
Translation	MVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000742.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000742.1

ORF Insert Sequence:

ATGGTGAATG GGGAACCTTT GCAATCGGCA CCACCTAACC CAAACCGTGA GGTGGCCGGA 
GACACCATCA TCTTCCGGGA CACCCAGATC AGCAGCAGGG CTGTGTACCA GTGCAACACC 
TCCAACGAGC ATGGCTACCT GCTGGCCAAC GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT 
CGGATGCTGT CGCCCCGGAA CCAGCTCATT CGAGTGATTC TTTACAACCG GACGCGGCTG 
GACTGCCCTT TCTTTGGGTC TCCCATCCCC ACACTGCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGTGGCAA CTACCATGTT TATGAGAACG GCAGTCTGGA AATTAAGATG 
ATCCGCAAAG AGGACCAGGG CATCTACACC TGTGTCGCCA CCAACATCCT GGGCAAAGCT 
GAAAACCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACCGGAT GCCCGAGGAC 
CAGGTGGCCA GAAGGGGCAC CACGGTGCAG CTGGAGTGTC GGGTGAAGCA CGACCCCTCC 
CTGAAACTCA CCGTCTCCTG GCTGAAGGAT GACGAGCCGC TCTATATTGG AAACAGGATG 
AAGAAGGAAG ACGACTCCCT GACCATCTTT GGGGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTGTCG CCAGCACCGA GCTAGACCAA GACCTGGCCA AGGCCTACCT CACCGTGCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTGC CCAAAGGACG GCCAGACCGG 
CCCCGGGACC TGGAGCTGAC CGACCTGGCC GAGAGGAGCG TGCGGCTGAC CTGGATCCCC 
GGGGATGCTA ACAACAGCCC CATCACAGAC TACGTCGTCC AGTTTGAAGA AGACCAGTTC 
CAACCTGGGG TCTGGCATGA CCATTCCAAG TACCCCGGCA GCGTTAACTC AGCCGTCCTC 
CGGCTGTCCC CGTATGTCAA CTACCAGTTC CGTGTCATTG CCATCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTCCCATC CGAGCGCTAC CGAACCAGTG GAGCACCCCC CGAGTCCAAT 
CCTGGTGACG TGAAGGGAGA GGGGACCAGA AAGAACAACA TGGAGATCAC GTGGACGCCC 
ATGAATGCCA CCTCGGCCTT TGGCCCCAAC CTGCGCTACA TTGTCAAGTG GAGGCGGAGA 
GAGACTCGAG AGGCCTGGAA CAACGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACCCCAGTCT ACGTGCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT CGGGAAGGGC 
CCTGAGCCAG AGTCCGTCAT CGGTTACTCC GGAGAAGATT TACCCAGTGC CCCTAGGCGT 
TTCCGAGTCC GGCAGCCCAA CCTGGAGACA ATCAACCTGG AATGGGATCA TCCTGAGCAT 
CCAAATGGGA TCATGATTGG ATACACTCTC AAATATGTGG CCTTTAACGG GACCAAAGTA 
GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC AATCAGACCA AGTTCACGGT GCAAAGAACG 
GACCCCGTGT CACGCTACCG CTTTACCCTC AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA 
GCCGTCACAG AGGAGTCACC AGCACCCCCG AATGAAGCTA CTCCAACCGC AGCTCCTCCC 
ACATTGCCCC CGACTACCGT GGGTGCGACG GGCGCTGTGA GCAGTACCGA TGCTACTGCC 
ATTGCTGCCA CCACCGAAGC CACAACAGTC CCCATCATCC CAACTGTCGC ACCTACCACC 
ATCGCCACCA CCACCACCGT CGCCACAACT ACTACAACCA CTGCTGCCGC CACCACCACC 
ACGGAGAGTC CTCCCACCAC CACCTCCGGG ACTAAGATAC ACGAATCCGC CCCTGATGAG 
CAGTCCATAT GGAACGTCAC GGTGCTCCCC AACAGTAAAT GGGCCAACAT CACCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA TCGACAGCAA CCATACGAAA 
AAAACTGTCC CAGTTAAGGC CCAGGCTCAG CCTATACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGGGT TTATTCCCGG GACAACGAGG GCATCAGCAG TACCGTCATC 
ACCTTTATGA CCAGTACAGC TTACACCAAC AACCAAGCGG ACATCGCCAC CCAGGGCTGG 
TTCATTGGGC TTATGTGCGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCA GTACGAGAAA AGAAGGATGT TCCCCTTGGC 
CCTGAAGACC CCAAGGAAGA GGATGGCTCA TTTGACTATA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GTCAGACATC TCTGGACGGC ACCATCAAGC AGCAGGAGAG TGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGT CAGTTCAATG AAGACGGCTC CTTCATCGGC 
CAGTACACGG TCAAAAAGGA CAAGGAGGAA ACAGAGGGCA ACGAAAGCTC AGAGGCCACG 
TCACCTGTCA ATGCTATCTA CTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000743.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856232.1 Homo sapiens neurofascin (NFASC), transcript variant X36, mRNA.

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CloneID	OHu87061
Clone ID Related Accession (Same CDS sequence)	XM_017000743.1
Accession Version	XM_017000743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACACCA 
AGCTTCATGT ATCCCCAGGG CACCGCGAGC AGCCAGATGG TGCTTCGTGG CATGGACCTC 
CTGCTGGAAT GCATCGCCTC CGGGGTCCCA ACACCAGACA TCGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGATAA GGCCAAGTTT GAGAACTTTA ATAAGGCCCT GCGTATCACA 
AATGTCTCTG AGGAAGACTC CGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCCT ACTGGCTGGA CGAACCCAAG 
AACCTTATTC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGAAACCCC 
AAACCCACTG TCCAGTGGAT GGTGAATGGG GAACCTTTGC AATCGGCACC ACCTAACCCA 
AACCGTGAGG TGGCCGGAGA CACCATCATC TTCCGGGACA CCCAGATCAG CAGCAGGGCT 
GTGTACCAGT GCAACACCTC CAACGAGCAT GGCTACCTGC TGGCCAACGC CTTTGTCAGT 
GTGCTGGATG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATTCG AGTGATTCTT 
TACAACCGGA CGCGGCTGGA CTGCCCTTTC TTTGGGTCTC CCATCCCCAC ACTGCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGCAACT ACCATGTTTA TGAGAACGGC 
AGTCTGGAAA TTAAGATGAT CCGCAAAGAG GACCAGGGCA TCTACACCTG TGTCGCCACC 
AACATCCTGG GCAAAGCTGA AAACCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACCGGATGC CCGAGGACCA GGTGGCCAGA AGGGGCACCA CGGTGCAGCT GGAGTGTCGG 
GTGAAGCACG ACCCCTCCCT GAAACTCACC GTCTCCTGGC TGAAGGATGA CGAGCCGCTC 
TATATTGGAA ACAGGATGAA GAAGGAAGAC GACTCCCTGA CCATCTTTGG GGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTGTCGCC AGCACCGAGC TAGACCAAGA CCTGGCCAAG 
GCCTACCTCA CCGTGCTAGG TAACTGCCCA TGCTCACCCT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856232.1
CDS	25..2172
Translation	MRRLAARGAAGDAGEVAAPAADSSDSAQGRSPSPWRLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCPCSPWH

Target ORF information:

RefSeq Version	XM_017000743.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000743.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACACCA 
AGCTTCATGT ATCCCCAGGG CACCGCGAGC AGCCAGATGG TGCTTCGTGG CATGGACCTC 
CTGCTGGAAT GCATCGCCTC CGGGGTCCCA ACACCAGACA TCGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGATAA GGCCAAGTTT GAGAACTTTA ATAAGGCCCT GCGTATCACA 
AATGTCTCTG AGGAAGACTC CGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCCT ACTGGCTGGA CGAACCCAAG 
AACCTTATTC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGAAACCCC 
AAACCCACTG TCCAGTGGAT GGTGAATGGG GAACCTTTGC AATCGGCACC ACCTAACCCA 
AACCGTGAGG TGGCCGGAGA CACCATCATC TTCCGGGACA CCCAGATCAG CAGCAGGGCT 
GTGTACCAGT GCAACACCTC CAACGAGCAT GGCTACCTGC TGGCCAACGC CTTTGTCAGT 
GTGCTGGATG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATTCG AGTGATTCTT 
TACAACCGGA CGCGGCTGGA CTGCCCTTTC TTTGGGTCTC CCATCCCCAC ACTGCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGCAACT ACCATGTTTA TGAGAACGGC 
AGTCTGGAAA TTAAGATGAT CCGCAAAGAG GACCAGGGCA TCTACACCTG TGTCGCCACC 
AACATCCTGG GCAAAGCTGA AAACCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACCGGATGC CCGAGGACCA GGTGGCCAGA AGGGGCACCA CGGTGCAGCT GGAGTGTCGG 
GTGAAGCACG ACCCCTCCCT GAAACTCACC GTCTCCTGGC TGAAGGATGA CGAGCCGCTC 
TATATTGGAA ACAGGATGAA GAAGGAAGAC GACTCCCTGA CCATCTTTGG GGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTGTCGCC AGCACCGAGC TAGACCAAGA CCTGGCCAAG 
GCCTACCTCA CCGTGCTAGG TAACTGCCCA TGCTCACCCT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509311.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507613.1 Homo sapiens neurofascin (NFASC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu65458
Clone ID Related Accession (Same CDS sequence)	XM_011509311.1
Accession Version	XM_011509311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TCCTCCCACA TTGCCCCCGA CTACCGTGGG TGCGACGGGC 
GCTGTGAGCA GTACCGATGC TACTGCCATT GCTGCCACCA CCGAAGCCAC AACAGTCCCC 
ATCATCCCAA CTGTCGCACC TACCACCATC GCCACCACCA CCACCGTCGC CACAACTACT 
ACAACCACTG CTGCCGCCAC CACCACCACG GAGAGTCCTC CCACCACCAC CTCCGGGACT 
AAGATACACG AATCCGCCCC TGATGAGCAG TCCATATGGA ACGTCACGGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT 
GAGTACATCG ACAGCAACCA TACGAAAAAA ACTGTCCCAG TTAAGGCCCA GGCTCAGCCT 
ATACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGGGTTTA TTCCCGGGAC 
AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAACAAC 
CAAGCGGACA TCGCCACCCA GGGCTGGTTC ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCAGTA 
CGAGAAAAGA AGGATGTTCC CCTTGGCCCT GAAGACCCCA AGGAAGAGGA TGGCTCATTT 
GACTATAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGTC AGACATCTCT GGACGGCACC 
ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGTCAG 
TTCAATGAAG ACGGCTCCTT CATCGGCCAG TACACGGTCA AAAAGGACAA GGAGGAAACA 
GAGGGCAACG AAAGCTCAGA GGCCACGTCA CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507613.1
CDS	91..4170
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509311.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509311.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TCCTCCCACA TTGCCCCCGA CTACCGTGGG TGCGACGGGC 
GCTGTGAGCA GTACCGATGC TACTGCCATT GCTGCCACCA CCGAAGCCAC AACAGTCCCC 
ATCATCCCAA CTGTCGCACC TACCACCATC GCCACCACCA CCACCGTCGC CACAACTACT 
ACAACCACTG CTGCCGCCAC CACCACCACG GAGAGTCCTC CCACCACCAC CTCCGGGACT 
AAGATACACG AATCCGCCCC TGATGAGCAG TCCATATGGA ACGTCACGGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT 
GAGTACATCG ACAGCAACCA TACGAAAAAA ACTGTCCCAG TTAAGGCCCA GGCTCAGCCT 
ATACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGGGTTTA TTCCCGGGAC 
AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAACAAC 
CAAGCGGACA TCGCCACCCA GGGCTGGTTC ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCAGTA 
CGAGAAAAGA AGGATGTTCC CCTTGGCCCT GAAGACCCCA AGGAAGAGGA TGGCTCATTT 
GACTATAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGTC AGACATCTCT GGACGGCACC 
ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGTCAG 
TTCAATGAAG ACGGCTCCTT CATCGGCCAG TACACGGTCA AAAAGGACAA GGAGGAAACA 
GAGGGCAACG AAAGCTCAGA GGCCACGTCA CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509312.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507614.1 Homo sapiens neurofascin (NFASC), transcript variant X3, mRNA.

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CloneID	OHu65459
Clone ID Related Accession (Same CDS sequence)	XM_011509312.1
Accession Version	XM_011509312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGCTGAT 
CAGGCCACTC CAACTAACCG TTTGGCTGCC CTGCCCAAAG GACGGCCAGA CCGGCCCCGG 
GACCTGGAGC TGACCGACCT GGCCGAGAGG AGCGTGCGGC TGACCTGGAT CCCCGGGGAT 
GCTAACAACA GCCCCATCAC AGACTACGTC GTCCAGTTTG AAGAAGACCA GTTCCAACCT 
GGGGTCTGGC ATGACCATTC CAAGTACCCC GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG 
TCCCCGTATG TCAACTACCA GTTCCGTGTC ATTGCCATCA ACGAGGTTGG GAGCAGCCAC 
CCCAGCCTCC CATCCGAGCG CTACCGAACC AGTGGAGCAC CCCCCGAGTC CAATCCTGGT 
GACGTGAAGG GAGAGGGGAC CAGAAAGAAC AACATGGAGA TCACGTGGAC GCCCATGAAT 
GCCACCTCGG CCTTTGGCCC CAACCTGCGC TACATTGTCA AGTGGAGGCG GAGAGAGACT 
CGAGAGGCCT GGAACAACGT CACAGTGTGG GGCTCTCGCT ACGTGGTGGG GCAGACCCCA 
GTCTACGTGC CCTATGAGAT CCGAGTCCAG GCTGAAAATG ACTTCGGGAA GGGCCCTGAG 
CCAGAGTCCG TCATCGGTTA CTCCGGAGAA GATTATCCCA GGGCTGCGCC CACTGAAGTT 
AAAGTCCGAG TCATGAACAG CACAGCCATC AGCCTTCAGT GGAACCGCGT CTACTCCGAC 
ACGGTCCAGG GCCAGCTCAG AGAGTACCGA GCCTACTACT GGAGGGAGAG CAGCTTGCTG 
AAGAACCTGT GGGTGTCTCA GAAGAGACAG CAAGCCAGCT TCCCTGGTGA CCGCCTCCGT 
GGCGTGGTGT CCCGCCTCTT CCCCTACAGT AACTACAAGC TGGAGATGGT TGTGGTCAAT 
GGGAGAGGTG ATGGGCCTCG CAGTGAGACC AAGGAGTTCA CCACCCCGGA AGGAGTACCC 
AGTGCCCCTA GGCGTTTCCG AGTCCGGCAG CCCAACCTGG AGACAATCAA CCTGGAATGG 
GATCATCCTG AGCATCCAAA TGGGATCATG ATTGGATACA CTCTCAAATA TGTGGCCTTT 
AACGGGACCA AAGTAGGAAA GCAGATAGTG GAAAACTTCT CTCCCAATCA GACCAAGTTC 
ACGGTGCAAA GAACGGACCC CGTGTCACGC TACCGCTTTA CCCTCAGCGC CAGGACGCAG 
GTGGGCTCTG GGGAAGCCGT CACAGAGGAG TCACCAGCAC CCCCGAATGA AGCTACTCCA 
ACCGCAGCTC CTCCCACATT GCCCCCGACT ACCGTGGGTG CGACGGGCGC TGTGAGCAGT 
ACCGATGCTA CTGCCATTGC TGCCACCACC GAAGCCACAA CAGTCCCCAT CATCCCAACT 
GTCGCACCTA CCACCATCGC CACCACCACC ACCGTCGCCA CAACTACTAC AACCACTGCT 
GCCGCCACCA CCACCACGGA GAGTCCTCCC ACCACCACCT CCGGGACTAA GATACACGAA 
TCCGCCCCTG ATGAGCAGTC CATATGGAAC GTCACGGTGC TCCCCAACAG TAAATGGGCC 
AACATCACCT GGAAGCACAA TTTCGGGCCC GGAACTGACT TTGTGGTTGA GTACATCGAC 
AGCAACCATA CGAAAAAAAC TGTCCCAGTT AAGGCCCAGG CTCAGCCTAT ACAGCTGACA 
GACCTCTATC CCGGGATGAC ATACACGTTG CGGGTTTATT CCCGGGACAA CGAGGGCATC 
AGCAGTACCG TCATCACCTT TATGACCAGT ACAGCTTACA CCAACAACCA AGCGGACATC 
GCCACCCAGG GCTGGTTCAT TGGGCTTATG TGCGCCATCG CCCTCCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCAGTACG AGAAAAGAAG 
GATGTTCCCC TTGGCCCTGA AGACCCCAAG GAAGAGGATG GCTCATTTGA CTATAGTGAT 
GAGGACAACA AGCCCCTGCA GGGCAGTCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG 
GAGAGTGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGTCAGTT CAATGAAGAC 
GGCTCCTTCA TCGGCCAGTA CACGGTCAAA AAGGACAAGG AGGAAACAGA GGGCAACGAA 
AGCTCAGAGG CCACGTCACC TGTCAATGCT ATCTACTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507614.1
CDS	178..4485
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509312.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509312.1

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGCTGAT 
CAGGCCACTC CAACTAACCG TTTGGCTGCC CTGCCCAAAG GACGGCCAGA CCGGCCCCGG 
GACCTGGAGC TGACCGACCT GGCCGAGAGG AGCGTGCGGC TGACCTGGAT CCCCGGGGAT 
GCTAACAACA GCCCCATCAC AGACTACGTC GTCCAGTTTG AAGAAGACCA GTTCCAACCT 
GGGGTCTGGC ATGACCATTC CAAGTACCCC GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG 
TCCCCGTATG TCAACTACCA GTTCCGTGTC ATTGCCATCA ACGAGGTTGG GAGCAGCCAC 
CCCAGCCTCC CATCCGAGCG CTACCGAACC AGTGGAGCAC CCCCCGAGTC CAATCCTGGT 
GACGTGAAGG GAGAGGGGAC CAGAAAGAAC AACATGGAGA TCACGTGGAC GCCCATGAAT 
GCCACCTCGG CCTTTGGCCC CAACCTGCGC TACATTGTCA AGTGGAGGCG GAGAGAGACT 
CGAGAGGCCT GGAACAACGT CACAGTGTGG GGCTCTCGCT ACGTGGTGGG GCAGACCCCA 
GTCTACGTGC CCTATGAGAT CCGAGTCCAG GCTGAAAATG ACTTCGGGAA GGGCCCTGAG 
CCAGAGTCCG TCATCGGTTA CTCCGGAGAA GATTATCCCA GGGCTGCGCC CACTGAAGTT 
AAAGTCCGAG TCATGAACAG CACAGCCATC AGCCTTCAGT GGAACCGCGT CTACTCCGAC 
ACGGTCCAGG GCCAGCTCAG AGAGTACCGA GCCTACTACT GGAGGGAGAG CAGCTTGCTG 
AAGAACCTGT GGGTGTCTCA GAAGAGACAG CAAGCCAGCT TCCCTGGTGA CCGCCTCCGT 
GGCGTGGTGT CCCGCCTCTT CCCCTACAGT AACTACAAGC TGGAGATGGT TGTGGTCAAT 
GGGAGAGGTG ATGGGCCTCG CAGTGAGACC AAGGAGTTCA CCACCCCGGA AGGAGTACCC 
AGTGCCCCTA GGCGTTTCCG AGTCCGGCAG CCCAACCTGG AGACAATCAA CCTGGAATGG 
GATCATCCTG AGCATCCAAA TGGGATCATG ATTGGATACA CTCTCAAATA TGTGGCCTTT 
AACGGGACCA AAGTAGGAAA GCAGATAGTG GAAAACTTCT CTCCCAATCA GACCAAGTTC 
ACGGTGCAAA GAACGGACCC CGTGTCACGC TACCGCTTTA CCCTCAGCGC CAGGACGCAG 
GTGGGCTCTG GGGAAGCCGT CACAGAGGAG TCACCAGCAC CCCCGAATGA AGCTACTCCA 
ACCGCAGCTC CTCCCACATT GCCCCCGACT ACCGTGGGTG CGACGGGCGC TGTGAGCAGT 
ACCGATGCTA CTGCCATTGC TGCCACCACC GAAGCCACAA CAGTCCCCAT CATCCCAACT 
GTCGCACCTA CCACCATCGC CACCACCACC ACCGTCGCCA CAACTACTAC AACCACTGCT 
GCCGCCACCA CCACCACGGA GAGTCCTCCC ACCACCACCT CCGGGACTAA GATACACGAA 
TCCGCCCCTG ATGAGCAGTC CATATGGAAC GTCACGGTGC TCCCCAACAG TAAATGGGCC 
AACATCACCT GGAAGCACAA TTTCGGGCCC GGAACTGACT TTGTGGTTGA GTACATCGAC 
AGCAACCATA CGAAAAAAAC TGTCCCAGTT AAGGCCCAGG CTCAGCCTAT ACAGCTGACA 
GACCTCTATC CCGGGATGAC ATACACGTTG CGGGTTTATT CCCGGGACAA CGAGGGCATC 
AGCAGTACCG TCATCACCTT TATGACCAGT ACAGCTTACA CCAACAACCA AGCGGACATC 
GCCACCCAGG GCTGGTTCAT TGGGCTTATG TGCGCCATCG CCCTCCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCAGTACG AGAAAAGAAG 
GATGTTCCCC TTGGCCCTGA AGACCCCAAG GAAGAGGATG GCTCATTTGA CTATAGTGAT 
GAGGACAACA AGCCCCTGCA GGGCAGTCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG 
GAGAGTGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGTCAGTT CAATGAAGAC 
GGCTCCTTCA TCGGCCAGTA CACGGTCAAA AAGGACAAGG AGGAAACAGA GGGCAACGAA 
AGCTCAGAGG CCACGTCACC TGTCAATGCT ATCTACTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509313.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507615.1 Homo sapiens neurofascin (NFASC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu65460
Clone ID Related Accession (Same CDS sequence)	XM_011509313.1
Accession Version	XM_011509313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGGACGGCCA GACCGGCCCC GGGACCTGGA GCTGACCGAC 
CTGGCCGAGA GGAGCGTGCG GCTGACCTGG ATCCCCGGGG ATGCTAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAAC CTGGGGTCTG GCATGACCAT 
TCCAAGTACC CCGGCAGCGT TAACTCAGCC GTCCTCCGGC TGTCCCCGTA TGTCAACTAC 
CAGTTCCGTG TCATTGCCAT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT CCCATCCGAG 
CGCTACCGAA CCAGTGGAGC ACCCCCCGAG TCCAATCCTG GTGACGTGAA GGGAGAGGGG 
ACCAGAAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC GGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGAGG CGGAGAGAGA CTCGAGAGGC CTGGAACAAC 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACCC CAGTCTACGT GCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTCGGG AAGGGCCCTG AGCCAGAGTC CGTCATCGGT 
TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAGTCCG AGTCATGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAGTACC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAAGCCAG CTTCCCTGGT GACCGCCTCC GTGGCGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGCAGTGAGA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC TAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA 
AATGGGATCA TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC 
CCCGTGTCAC GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC 
GTCACAGAGG AGTCACCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TGCGACGGGC GCTGTGAGCA GTACCGATGC TACTGCCATT 
GCTGCCACCA CCGAAGCCAC AACAGTCCCC ATCATCCCAA CTGTCGCACC TACCACCATC 
GCCACCACCA CCACCGTCGC CACAACTACT ACAACCACTG CTGCCGCCAC CACCACCACG 
GAGAGTCCTC CCACCACCAC CTCCGGGACT AAGATACACG AATCCGCCCC TGATGAGCAG 
TCCATATGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCAG TTAAGGCCCA GGCTCAGCCT ATACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGGGTTTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507615.1
CDS	91..4140
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509313.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509313.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGGACGGCCA GACCGGCCCC GGGACCTGGA GCTGACCGAC 
CTGGCCGAGA GGAGCGTGCG GCTGACCTGG ATCCCCGGGG ATGCTAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAAC CTGGGGTCTG GCATGACCAT 
TCCAAGTACC CCGGCAGCGT TAACTCAGCC GTCCTCCGGC TGTCCCCGTA TGTCAACTAC 
CAGTTCCGTG TCATTGCCAT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT CCCATCCGAG 
CGCTACCGAA CCAGTGGAGC ACCCCCCGAG TCCAATCCTG GTGACGTGAA GGGAGAGGGG 
ACCAGAAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC GGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGAGG CGGAGAGAGA CTCGAGAGGC CTGGAACAAC 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACCC CAGTCTACGT GCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTCGGG AAGGGCCCTG AGCCAGAGTC CGTCATCGGT 
TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAGTCCG AGTCATGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAGTACC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAAGCCAG CTTCCCTGGT GACCGCCTCC GTGGCGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGCAGTGAGA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC TAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA 
AATGGGATCA TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC 
CCCGTGTCAC GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC 
GTCACAGAGG AGTCACCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TGCGACGGGC GCTGTGAGCA GTACCGATGC TACTGCCATT 
GCTGCCACCA CCGAAGCCAC AACAGTCCCC ATCATCCCAA CTGTCGCACC TACCACCATC 
GCCACCACCA CCACCGTCGC CACAACTACT ACAACCACTG CTGCCGCCAC CACCACCACG 
GAGAGTCCTC CCACCACCAC CTCCGGGACT AAGATACACG AATCCGCCCC TGATGAGCAG 
TCCATATGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCAG TTAAGGCCCA GGCTCAGCCT ATACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGGGTTTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509314.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507616.1 Homo sapiens neurofascin (NFASC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu65461
Clone ID Related Accession (Same CDS sequence)	XM_011509314.1
Accession Version	XM_011509314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC 
CGGCCCCGGG ACCTGGAGCT GACCGACCTG GCCGAGAGGA GCGTGCGGCT GACCTGGATC 
CCCGGGGATG CTAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAAGACCAG 
TTCCAACCTG GGGTCTGGCA TGACCATTCC AAGTACCCCG GCAGCGTTAA CTCAGCCGTC 
CTCCGGCTGT CCCCGTATGT CAACTACCAG TTCCGTGTCA TTGCCATCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTCCC ATCCGAGCGC TACCGAACCA GTGGAGCACC CCCCGAGTCC 
AATCCTGGTG ACGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCCATGAATG CCACCTCGGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGAGGCGG 
AGAGAGACTC GAGAGGCCTG GAACAACGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACCCCAG TCTACGTGCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTCGGGAAG 
GGCCCTGAGC CAGAGTCCGT CATCGGTTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAGTCCGAGT CATGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTACTCCAA CCGCAGCTCC TCCCACATTG CCCCCGACTA CCGTGGGTGC GACGGGCGCT 
GTGAGCAGTA CCGATGCTAC TGCCATTGCT GCCACCACCG AAGCCACAAC AGTCCCCATC 
ATCCCAACTG TCGCACCTAC CACCATCGCC ACCACCACCA CCGTCGCCAC AACTACTACA 
ACCACTGCTG CCGCCACCAC CACCACGGAG AGTCCTCCCA CCACCACCTC CGGGACTAAG 
ATACACGAAT CCGCCCCTGA TGAGCAGTCC ATATGGAACG TCACGGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCGGGCCCG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCAGTTA AGGCCCAGGC TCAGCCTATA 
CAGCTGACAG ACCTCTATCC CGGGATGACA TACACGTTGC GGGTTTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAACAACCAA 
GCGGACATCG CCACCCAGGG CTGGTTCATT GGGCTTATGT GCGCCATCGC CCTCCTGGTG 
CTGATCCTGC TCATCGTCTG TTTCATCAAG AGGAGTCGCG GCGGCAAGTA CCCAGTACGA 
GAAAAGAAGG ATGTTCCCCT TGGCCCTGAA GACCCCAAGG AAGAGGATGG CTCATTTGAC 
TATAGTGATG AGGACAACAA GCCCCTGCAG GGCAGTCAGA CATCTCTGGA CGGCACCATC 
AAGCAGCAGG AGAGTGACGA CAGCCTGGTG GACTATGGCG AGGGTGGCGA GGGTCAGTTC 
AATGAAGACG GCTCCTTCAT CGGCCAGTAC ACGGTCAAAA AGGACAAGGA GGAAACAGAG 
GGCAACGAAA GCTCAGAGGC CACGTCACCT GTCAATGCTA TCTACTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507616.1
CDS	178..4434
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509314.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509314.1

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC 
CGGCCCCGGG ACCTGGAGCT GACCGACCTG GCCGAGAGGA GCGTGCGGCT GACCTGGATC 
CCCGGGGATG CTAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAAGACCAG 
TTCCAACCTG GGGTCTGGCA TGACCATTCC AAGTACCCCG GCAGCGTTAA CTCAGCCGTC 
CTCCGGCTGT CCCCGTATGT CAACTACCAG TTCCGTGTCA TTGCCATCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTCCC ATCCGAGCGC TACCGAACCA GTGGAGCACC CCCCGAGTCC 
AATCCTGGTG ACGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCCATGAATG CCACCTCGGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGAGGCGG 
AGAGAGACTC GAGAGGCCTG GAACAACGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACCCCAG TCTACGTGCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTCGGGAAG 
GGCCCTGAGC CAGAGTCCGT CATCGGTTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAGTCCGAGT CATGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTACTCCAA CCGCAGCTCC TCCCACATTG CCCCCGACTA CCGTGGGTGC GACGGGCGCT 
GTGAGCAGTA CCGATGCTAC TGCCATTGCT GCCACCACCG AAGCCACAAC AGTCCCCATC 
ATCCCAACTG TCGCACCTAC CACCATCGCC ACCACCACCA CCGTCGCCAC AACTACTACA 
ACCACTGCTG CCGCCACCAC CACCACGGAG AGTCCTCCCA CCACCACCTC CGGGACTAAG 
ATACACGAAT CCGCCCCTGA TGAGCAGTCC ATATGGAACG TCACGGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCGGGCCCG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCAGTTA AGGCCCAGGC TCAGCCTATA 
CAGCTGACAG ACCTCTATCC CGGGATGACA TACACGTTGC GGGTTTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAACAACCAA 
GCGGACATCG CCACCCAGGG CTGGTTCATT GGGCTTATGT GCGCCATCGC CCTCCTGGTG 
CTGATCCTGC TCATCGTCTG TTTCATCAAG AGGAGTCGCG GCGGCAAGTA CCCAGTACGA 
GAAAAGAAGG ATGTTCCCCT TGGCCCTGAA GACCCCAAGG AAGAGGATGG CTCATTTGAC 
TATAGTGATG AGGACAACAA GCCCCTGCAG GGCAGTCAGA CATCTCTGGA CGGCACCATC 
AAGCAGCAGG AGAGTGACGA CAGCCTGGTG GACTATGGCG AGGGTGGCGA GGGTCAGTTC 
AATGAAGACG GCTCCTTCAT CGGCCAGTAC ACGGTCAAAA AGGACAAGGA GGAAACAGAG 
GGCAACGAAA GCTCAGAGGC CACGTCACCT GTCAATGCTA TCTACTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509315.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507617.1 Homo sapiens neurofascin (NFASC), transcript variant X6, mRNA.

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CloneID	OHu65462
Clone ID Related Accession (Same CDS sequence)	XM_011509315.1
Accession Version	XM_011509315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACCGGCCCCG GGACCTGGAG 
CTGACCGACC TGGCCGAGAG GAGCGTGCGG CTGACCTGGA TCCCCGGGGA TGCTAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAACC TGGGGTCTGG 
CATGACCATT CCAAGTACCC CGGCAGCGTT AACTCAGCCG TCCTCCGGCT GTCCCCGTAT 
GTCAACTACC AGTTCCGTGT CATTGCCATC AACGAGGTTG GGAGCAGCCA CCCCAGCCTC 
CCATCCGAGC GCTACCGAAC CAGTGGAGCA CCCCCCGAGT CCAATCCTGG TGACGTGAAG 
GGAGAGGGGA CCAGAAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCG 
GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGAGGC GGAGAGAGAC TCGAGAGGCC 
TGGAACAACG TCACAGTGTG GGGCTCTCGC TACGTGGTGG GGCAGACCCC AGTCTACGTG 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTCGGGA AGGGCCCTGA GCCAGAGTCC 
GTCATCGGTT ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACTGAAGT TAAAGTCCGA 
GTCATGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG 
GGCCAGCTCA GAGAGTACCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGACA GCAAGCCAGC TTCCCTGGTG ACCGCCTCCG TGGCGTGGTG 
TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGAGAGGT 
GATGGGCCTC GCAGTGAGAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCT 
AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCT 
GAGCATCCAA ATGGGATCAT GATTGGATAC ACTCTCAAAT ATGTGGCCTT TAACGGGACC 
AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA 
AGAACGGACC CCGTGTCACG CTACCGCTTT ACCCTCAGCG CCAGGACGCA GGTGGGCTCT 
GGGGAAGCCG TCACAGAGGA GTCACCAGCA CCCCCGAATG AAGCTACTCC AACCGCAGCT 
CCTCCCACAT TGCCCCCGAC TACCGTGGGT GCGACGGGCG CTGTGAGCAG TACCGATGCT 
ACTGCCATTG CTGCCACCAC CGAAGCCACA ACAGTCCCCA TCATCCCAAC TGTCGCACCT 
ACCACCATCG CCACCACCAC CACCGTCGCC ACAACTACTA CAACCACTGC TGCCGCCACC 
ACCACCACGG AGAGTCCTCC CACCACCACC TCCGGGACTA AGATACACGA ATCCGCCCCT 
GATGAGCAGT CCATATGGAA CGTCACGGTG CTCCCCAACA GTAAATGGGC CAACATCACC 
TGGAAGCACA ATTTCGGGCC CGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT 
ACGAAAAAAA CTGTCCCAGT TAAGGCCCAG GCTCAGCCTA TACAGCTGAC AGACCTCTAT 
CCCGGGATGA CATACACGTT GCGGGTTTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC 
GTCATCACCT TTATGACCAG TACAGCTTAC ACCAACAACC AAGCGGACAT CGCCACCCAG 
GGCTGGTTCA TTGGGCTTAT GTGCGCCATC GCCCTCCTGG TGCTGATCCT GCTCATCGTC 
TGTTTCATCA AGAGGAGTCG CGGCGGCAAG TACCCAGTAC GAGAAAAGAA GGATGTTCCC 
CTTGGCCCTG AAGACCCCAA GGAAGAGGAT GGCTCATTTG ACTATAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGTCA GACATCTCTG GACGGCACCA TCAAGCAGCA GGAGAGTGAC 
GACAGCCTGG TGGACTATGG CGAGGGTGGC GAGGGTCAGT TCAATGAAGA CGGCTCCTTC 
ATCGGCCAGT ACACGGTCAA AAAGGACAAG GAGGAAACAG AGGGCAACGA AAGCTCAGAG 
GCCACGTCAC CTGTCAATGC TATCTACTCT CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507617.1
CDS	91..4089
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509315.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509315.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACCGGCCCCG GGACCTGGAG 
CTGACCGACC TGGCCGAGAG GAGCGTGCGG CTGACCTGGA TCCCCGGGGA TGCTAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAACC TGGGGTCTGG 
CATGACCATT CCAAGTACCC CGGCAGCGTT AACTCAGCCG TCCTCCGGCT GTCCCCGTAT 
GTCAACTACC AGTTCCGTGT CATTGCCATC AACGAGGTTG GGAGCAGCCA CCCCAGCCTC 
CCATCCGAGC GCTACCGAAC CAGTGGAGCA CCCCCCGAGT CCAATCCTGG TGACGTGAAG 
GGAGAGGGGA CCAGAAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCG 
GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGAGGC GGAGAGAGAC TCGAGAGGCC 
TGGAACAACG TCACAGTGTG GGGCTCTCGC TACGTGGTGG GGCAGACCCC AGTCTACGTG 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTCGGGA AGGGCCCTGA GCCAGAGTCC 
GTCATCGGTT ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACTGAAGT TAAAGTCCGA 
GTCATGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG 
GGCCAGCTCA GAGAGTACCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGACA GCAAGCCAGC TTCCCTGGTG ACCGCCTCCG TGGCGTGGTG 
TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGAGAGGT 
GATGGGCCTC GCAGTGAGAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCT 
AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCT 
GAGCATCCAA ATGGGATCAT GATTGGATAC ACTCTCAAAT ATGTGGCCTT TAACGGGACC 
AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA 
AGAACGGACC CCGTGTCACG CTACCGCTTT ACCCTCAGCG CCAGGACGCA GGTGGGCTCT 
GGGGAAGCCG TCACAGAGGA GTCACCAGCA CCCCCGAATG AAGCTACTCC AACCGCAGCT 
CCTCCCACAT TGCCCCCGAC TACCGTGGGT GCGACGGGCG CTGTGAGCAG TACCGATGCT 
ACTGCCATTG CTGCCACCAC CGAAGCCACA ACAGTCCCCA TCATCCCAAC TGTCGCACCT 
ACCACCATCG CCACCACCAC CACCGTCGCC ACAACTACTA CAACCACTGC TGCCGCCACC 
ACCACCACGG AGAGTCCTCC CACCACCACC TCCGGGACTA AGATACACGA ATCCGCCCCT 
GATGAGCAGT CCATATGGAA CGTCACGGTG CTCCCCAACA GTAAATGGGC CAACATCACC 
TGGAAGCACA ATTTCGGGCC CGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT 
ACGAAAAAAA CTGTCCCAGT TAAGGCCCAG GCTCAGCCTA TACAGCTGAC AGACCTCTAT 
CCCGGGATGA CATACACGTT GCGGGTTTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC 
GTCATCACCT TTATGACCAG TACAGCTTAC ACCAACAACC AAGCGGACAT CGCCACCCAG 
GGCTGGTTCA TTGGGCTTAT GTGCGCCATC GCCCTCCTGG TGCTGATCCT GCTCATCGTC 
TGTTTCATCA AGAGGAGTCG CGGCGGCAAG TACCCAGTAC GAGAAAAGAA GGATGTTCCC 
CTTGGCCCTG AAGACCCCAA GGAAGAGGAT GGCTCATTTG ACTATAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGTCA GACATCTCTG GACGGCACCA TCAAGCAGCA GGAGAGTGAC 
GACAGCCTGG TGGACTATGG CGAGGGTGGC GAGGGTCAGT TCAATGAAGA CGGCTCCTTC 
ATCGGCCAGT ACACGGTCAA AAAGGACAAG GAGGAAACAG AGGGCAACGA AAGCTCAGAG 
GCCACGTCAC CTGTCAATGC TATCTACTCT CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509316.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507618.1 Homo sapiens neurofascin (NFASC), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu65463
Clone ID Related Accession (Same CDS sequence)	XM_011509316.1
Accession Version	XM_011509316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTGCGA CGGGCGCTGT GAGCAGTACC GATGCTACTG CCATTGCTGC CACCACCGAA 
GCCACAACAG TCCCCATCAT CCCAACTGTC GCACCTACCA CCATCGCCAC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCTGCC GCCACCACCA CCACGGAGAG TCCTCCCACC 
ACCACCTCCG GGACTAAGAT ACACGAATCC GCCCCTGATG AGCAGTCCAT ATGGAACGTC 
ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CGGGCCCGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCAGTTAAG 
GCCCAGGCTC AGCCTATACA GCTGACAGAC CTCTATCCCG GGATGACATA CACGTTGCGG 
GTTTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ACAACCAAGC GGACATCGCC ACCCAGGGCT GGTTCATTGG GCTTATGTGC 
GCCATCGCCC TCCTGGTGCT GATCCTGCTC ATCGTCTGTT TCATCAAGAG GAGTCGCGGC 
GGCAAGTACC CAGTACGAGA AAAGAAGGAT GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA 
GAGGATGGCT CATTTGACTA TAGTGATGAG GACAACAAGC CCCTGCAGGG CAGTCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTGGTGGA CTATGGCGAG 
GGTGGCGAGG GTCAGTTCAA TGAAGACGGC TCCTTCATCG GCCAGTACAC GGTCAAAAAG 
GACAAGGAGG AAACAGAGGG CAACGAAAGC TCAGAGGCCA CGTCACCTGT CAATGCTATC 
TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507618.1
CDS	288..4382
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509316.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509316.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTGCGA CGGGCGCTGT GAGCAGTACC GATGCTACTG CCATTGCTGC CACCACCGAA 
GCCACAACAG TCCCCATCAT CCCAACTGTC GCACCTACCA CCATCGCCAC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCTGCC GCCACCACCA CCACGGAGAG TCCTCCCACC 
ACCACCTCCG GGACTAAGAT ACACGAATCC GCCCCTGATG AGCAGTCCAT ATGGAACGTC 
ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CGGGCCCGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCAGTTAAG 
GCCCAGGCTC AGCCTATACA GCTGACAGAC CTCTATCCCG GGATGACATA CACGTTGCGG 
GTTTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ACAACCAAGC GGACATCGCC ACCCAGGGCT GGTTCATTGG GCTTATGTGC 
GCCATCGCCC TCCTGGTGCT GATCCTGCTC ATCGTCTGTT TCATCAAGAG GAGTCGCGGC 
GGCAAGTACC CAGTACGAGA AAAGAAGGAT GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA 
GAGGATGGCT CATTTGACTA TAGTGATGAG GACAACAAGC CCCTGCAGGG CAGTCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTGGTGGA CTATGGCGAG 
GGTGGCGAGG GTCAGTTCAA TGAAGACGGC TCCTTCATCG GCCAGTACAC GGTCAAAAAG 
GACAAGGAGG AAACAGAGGG CAACGAAAGC TCAGAGGCCA CGTCACCTGT CAATGCTATC 
TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509317.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507619.1 Homo sapiens neurofascin (NFASC), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu65464
Clone ID Related Accession (Same CDS sequence)	XM_011509317.1
Accession Version	XM_011509317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTGCGA CGGGCGCTGT GAGCAGTACC GATGCTACTG CCATTGCTGC CACCACCGAA 
GCCACAACAG TCCCCATCAT CCCAACTGTC GCACCTACCA CCATCGCCAC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCTGCC GCCACCACCA CCACGGAGAG TCCTCCCACC 
ACCACCTCCG GGACTAAGAT ACACGAATCC GCCCCTGATG AGCAGTCCAT ATGGAACGTC 
ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CGGGCCCGGA 
ACTGACTTTG TGGTTGAGTA CATCGACACT TACACCAACA ACCAAGCGGA CATCGCCACC 
CAGGGCTGGT TCATTGGGCT TATGTGCGCC ATCGCCCTCC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCAG TACGAGAAAA GAAGGATGTT 
CCCCTTGGCC CTGAAGACCC CAAGGAAGAG GATGGCTCAT TTGACTATAG TGATGAGGAC 
AACAAGCCCC TGCAGGGCAG TCAGACATCT CTGGACGGCA CCATCAAGCA GCAGGAGAGT 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGTC AGTTCAATGA AGACGGCTCC 
TTCATCGGCC AGTACACGGT CAAAAAGGAC AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA 
GAGGCCACGT CACCTGTCAA TGCTATCTAC TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507619.1
CDS	91..4032
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDTYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509317.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509317.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTGCGA CGGGCGCTGT GAGCAGTACC GATGCTACTG CCATTGCTGC CACCACCGAA 
GCCACAACAG TCCCCATCAT CCCAACTGTC GCACCTACCA CCATCGCCAC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCTGCC GCCACCACCA CCACGGAGAG TCCTCCCACC 
ACCACCTCCG GGACTAAGAT ACACGAATCC GCCCCTGATG AGCAGTCCAT ATGGAACGTC 
ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CGGGCCCGGA 
ACTGACTTTG TGGTTGAGTA CATCGACACT TACACCAACA ACCAAGCGGA CATCGCCACC 
CAGGGCTGGT TCATTGGGCT TATGTGCGCC ATCGCCCTCC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCAG TACGAGAAAA GAAGGATGTT 
CCCCTTGGCC CTGAAGACCC CAAGGAAGAG GATGGCTCAT TTGACTATAG TGATGAGGAC 
AACAAGCCCC TGCAGGGCAG TCAGACATCT CTGGACGGCA CCATCAAGCA GCAGGAGAGT 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGTC AGTTCAATGA AGACGGCTCC 
TTCATCGGCC AGTACACGGT CAAAAAGGAC AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA 
GAGGCCACGT CACCTGTCAA TGCTATCTAC TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509318.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507620.1 Homo sapiens neurofascin (NFASC), transcript variant X11, mRNA.

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CloneID	OHu65465
Clone ID Related Accession (Same CDS sequence)	XM_011509318.2
Accession Version	XM_011509318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCAACCACC CTTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCAG AAAGAACACC AAGCTTCATG 
TATCCCCAGG GCACCGCGAG CAGCCAGATG GTGCTTCGTG GCATGGACCT CCTGCTGGAA 
TGCATCGCCT CCGGGGTCCC AACACCAGAC ATCGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTGATA AGGCCAAGTT TGAGAACTTT AATAAGGCCC TGCGTATCAC AAATGTCTCT 
GAGGAAGACT CCGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ACGAACCCAA GAACCTTATT 
CTGGCTCCTG GCGAGGATGG GAGACTGGTG TGTCGAGCCA ATGGAAACCC CAAACCCACT 
GTCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCTAACCC AAACCGTGAG 
GTGGCCGGAG ACACCATCAT CTTCCGGGAC ACCCAGATCA GCAGCAGGGC TGTGTACCAG 
TGCAACACCT CCAACGAGCA TGGCTACCTG CTGGCCAACG CCTTTGTCAG TGTGCTGGAT 
GTGCCGCCTC GGATGCTGTC GCCCCGGAAC CAGCTCATTC GAGTGATTCT TTACAACCGG 
ACGCGGCTGG ACTGCCCTTT CTTTGGGTCT CCCATCCCCA CACTGCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGTGGCAAC TACCATGTTT ATGAGAACGG CAGTCTGGAA 
ATTAAGATGA TCCGCAAAGA GGACCAGGGC ATCTACACCT GTGTCGCCAC CAACATCCTG 
GGCAAAGCTG AAAACCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACCGGATG 
CCCGAGGACC AGGTGGCCAG AAGGGGCACC ACGGTGCAGC TGGAGTGTCG GGTGAAGCAC 
GACCCCTCCC TGAAACTCAC CGTCTCCTGG CTGAAGGATG ACGAGCCGCT CTATATTGGA 
AACAGGATGA AGAAGGAAGA CGACTCCCTG ACCATCTTTG GGGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTGTCGC CAGCACCGAG CTAGACCAAG ACCTGGCCAA GGCCTACCTC 
ACCGTGCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTGCC CAAAGGACGG 
CCAGACCGGC CCCGGGACCT GGAGCTGACC GACCTGGCCG AGAGGAGCGT GCGGCTGACC 
TGGATCCCCG GGGATGCTAA CAACAGCCCC ATCACAGACT ACGTCGTCCA GTTTGAAGAA 
GACCAGTTCC AACCTGGGGT CTGGCATGAC CATTCCAAGT ACCCCGGCAG CGTTAACTCA 
GCCGTCCTCC GGCTGTCCCC GTATGTCAAC TACCAGTTCC GTGTCATTGC CATCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTCCCATCC GAGCGCTACC GAACCAGTGG AGCACCCCCC 
GAGTCCAATC CTGGTGACGT GAAGGGAGAG GGGACCAGAA AGAACAACAT GGAGATCACG 
TGGACGCCCA TGAATGCCAC CTCGGCCTTT GGCCCCAACC TGCGCTACAT TGTCAAGTGG 
AGGCGGAGAG AGACTCGAGA GGCCTGGAAC AACGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CCCCAGTCTA CGTGCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTC 
GGGAAGGGCC CTGAGCCAGA GTCCGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAGT CCGAGTCATG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGCGTCTACT CCGACACGGT CCAGGGCCAG CTCAGAGAGT ACCGAGCCTA CTACTGGAGG 
GAGAGCAGCT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAAGC CAGCTTCCCT 
GGTGACCGCC TCCGTGGCGT GGTGTCCCGC CTCTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTTGTGG TCAATGGGAG AGGTGATGGG CCTCGCAGTG AGACCAAGGA GTTCACCACC 
CCGGAAGGAG TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTGCGACG 
GGCGCTGTGA GCAGTACCGA TGCTACTGCC ATTGCTGCCA CCACCGAAGC CACAACAGTC 
CCCATCATCC CAACTGTCGC ACCTACCACC ATCGCCACCA CCACCACCGT CGCCACAACT 
ACTACAACCA CTGCTGCCGC CACCACCACC ACGGAGAGTC CTCCCACCAC CACCTCCGGG 
ACTAAGATAC ACGAATCCGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507620.1
CDS	25..4407
Translation	MRRLAARGAAGDAGEVAAPAADSSDSAQGRSPSPWRLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509318.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509318.2

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCAACCACC CTTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCAG AAAGAACACC AAGCTTCATG 
TATCCCCAGG GCACCGCGAG CAGCCAGATG GTGCTTCGTG GCATGGACCT CCTGCTGGAA 
TGCATCGCCT CCGGGGTCCC AACACCAGAC ATCGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTGATA AGGCCAAGTT TGAGAACTTT AATAAGGCCC TGCGTATCAC AAATGTCTCT 
GAGGAAGACT CCGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ACGAACCCAA GAACCTTATT 
CTGGCTCCTG GCGAGGATGG GAGACTGGTG TGTCGAGCCA ATGGAAACCC CAAACCCACT 
GTCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCTAACCC AAACCGTGAG 
GTGGCCGGAG ACACCATCAT CTTCCGGGAC ACCCAGATCA GCAGCAGGGC TGTGTACCAG 
TGCAACACCT CCAACGAGCA TGGCTACCTG CTGGCCAACG CCTTTGTCAG TGTGCTGGAT 
GTGCCGCCTC GGATGCTGTC GCCCCGGAAC CAGCTCATTC GAGTGATTCT TTACAACCGG 
ACGCGGCTGG ACTGCCCTTT CTTTGGGTCT CCCATCCCCA CACTGCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGTGGCAAC TACCATGTTT ATGAGAACGG CAGTCTGGAA 
ATTAAGATGA TCCGCAAAGA GGACCAGGGC ATCTACACCT GTGTCGCCAC CAACATCCTG 
GGCAAAGCTG AAAACCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACCGGATG 
CCCGAGGACC AGGTGGCCAG AAGGGGCACC ACGGTGCAGC TGGAGTGTCG GGTGAAGCAC 
GACCCCTCCC TGAAACTCAC CGTCTCCTGG CTGAAGGATG ACGAGCCGCT CTATATTGGA 
AACAGGATGA AGAAGGAAGA CGACTCCCTG ACCATCTTTG GGGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTGTCGC CAGCACCGAG CTAGACCAAG ACCTGGCCAA GGCCTACCTC 
ACCGTGCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTGCC CAAAGGACGG 
CCAGACCGGC CCCGGGACCT GGAGCTGACC GACCTGGCCG AGAGGAGCGT GCGGCTGACC 
TGGATCCCCG GGGATGCTAA CAACAGCCCC ATCACAGACT ACGTCGTCCA GTTTGAAGAA 
GACCAGTTCC AACCTGGGGT CTGGCATGAC CATTCCAAGT ACCCCGGCAG CGTTAACTCA 
GCCGTCCTCC GGCTGTCCCC GTATGTCAAC TACCAGTTCC GTGTCATTGC CATCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTCCCATCC GAGCGCTACC GAACCAGTGG AGCACCCCCC 
GAGTCCAATC CTGGTGACGT GAAGGGAGAG GGGACCAGAA AGAACAACAT GGAGATCACG 
TGGACGCCCA TGAATGCCAC CTCGGCCTTT GGCCCCAACC TGCGCTACAT TGTCAAGTGG 
AGGCGGAGAG AGACTCGAGA GGCCTGGAAC AACGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CCCCAGTCTA CGTGCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTC 
GGGAAGGGCC CTGAGCCAGA GTCCGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAGT CCGAGTCATG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGCGTCTACT CCGACACGGT CCAGGGCCAG CTCAGAGAGT ACCGAGCCTA CTACTGGAGG 
GAGAGCAGCT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAAGC CAGCTTCCCT 
GGTGACCGCC TCCGTGGCGT GGTGTCCCGC CTCTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTTGTGG TCAATGGGAG AGGTGATGGG CCTCGCAGTG AGACCAAGGA GTTCACCACC 
CCGGAAGGAG TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTGCGACG 
GGCGCTGTGA GCAGTACCGA TGCTACTGCC ATTGCTGCCA CCACCGAAGC CACAACAGTC 
CCCATCATCC CAACTGTCGC ACCTACCACC ATCGCCACCA CCACCACCGT CGCCACAACT 
ACTACAACCA CTGCTGCCGC CACCACCACC ACGGAGAGTC CTCCCACCAC CACCTCCGGG 
ACTAAGATAC ACGAATCCGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509319.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507621.1 Homo sapiens neurofascin (NFASC), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu65466
Clone ID Related Accession (Same CDS sequence)	XM_011509319.1
Accession Version	XM_011509319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT ATCCCAGGGC TGCGCCCACT GAAGTTAAAG TCCGAGTCAT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGCGTCTAC TCCGACACGG TCCAGGGCCA GCTCAGAGAG 
TACCGAGCCT ACTACTGGAG GGAGAGCAGC TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG 
AGACAGCAAG CCAGCTTCCC TGGTGACCGC CTCCGTGGCG TGGTGTCCCG CCTCTTCCCC 
TACAGTAACT ACAAGCTGGA GATGGTTGTG GTCAATGGGA GAGGTGATGG GCCTCGCAGT 
GAGACCAAGG AGTTCACCAC CCCGGAAGGA GTACCCAGTG CCCCTAGGCG TTTCCGAGTC 
CGGCAGCCCA ACCTGGAGAC AATCAACCTG GAATGGGATC ATCCTGAGCA TCCAAATGGG 
ATCATGATTG GATACACTCT CAAATATGTG GCCTTTAACG GGACCAAAGT AGGAAAGCAG 
ATAGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCACGG TGCAAAGAAC GGACCCCGTG 
TCACGCTACC GCTTTACCCT CAGCGCCAGG ACGCAGGTGG GCTCTGGGGA AGCCGTCACA 
GAGGAGTCAC CAGCACCCCC GAATGAAGCT ACTCCAACCG CAGCTCCTCC CACATTGCCC 
CCGACTACCG TGGGTGCGAC GGGCGCTGTG AGCAGTACCG ATGCTACTGC CATTGCTGCC 
ACCACCGAAG CCACAACAGT CCCCATCATC CCAACTGTCG CACCTACCAC CATCGCCACC 
ACCACCACCG TCGCCACAAC TACTACAACC ACTGCTGCCG CCACCACCAC CACGGAGAGT 
CCTCCCACCA CCACCTCCGG GACTAAGATA CACGAATCCG CCCCTGATGA GCAGTCCATA 
TGGAACGTCA CGGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC 
GGGCCCGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAACTGTC 
CCAGTTAAGG CCCAGGCTCA GCCTATACAG CTGACAGACC TCTATCCCGG GATGACATAC 
ACGTTGCGGG TTTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG 
ACCAGTACAG CTTACACCAA CAACCAAGCG GACATCGCCA CCCAGGGCTG GTTCATTGGG 
CTTATGTGCG CCATCGCCCT CCTGGTGCTG ATCCTGCTCA TCGTCTGTTT CATCAAGAGG 
AGTCGCGGCG GCAAGTACCC AGTACGAGAA AAGAAGGATG TTCCCCTTGG CCCTGAAGAC 
CCCAAGGAAG AGGATGGCTC ATTTGACTAT AGTGATGAGG ACAACAAGCC CCTGCAGGGC 
AGTCAGACAT CTCTGGACGG CACCATCAAG CAGCAGGAGA GTGACGACAG CCTGGTGGAC 
TATGGCGAGG GTGGCGAGGG TCAGTTCAAT GAAGACGGCT CCTTCATCGG CCAGTACACG 
GTCAAAAAGG ACAAGGAGGA AACAGAGGGC AACGAAAGCT CAGAGGCCAC GTCACCTGTC 
AATGCTATCT ACTCTCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507621.1
CDS	288..4331
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509319.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT ATCCCAGGGC TGCGCCCACT GAAGTTAAAG TCCGAGTCAT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGCGTCTAC TCCGACACGG TCCAGGGCCA GCTCAGAGAG 
TACCGAGCCT ACTACTGGAG GGAGAGCAGC TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG 
AGACAGCAAG CCAGCTTCCC TGGTGACCGC CTCCGTGGCG TGGTGTCCCG CCTCTTCCCC 
TACAGTAACT ACAAGCTGGA GATGGTTGTG GTCAATGGGA GAGGTGATGG GCCTCGCAGT 
GAGACCAAGG AGTTCACCAC CCCGGAAGGA GTACCCAGTG CCCCTAGGCG TTTCCGAGTC 
CGGCAGCCCA ACCTGGAGAC AATCAACCTG GAATGGGATC ATCCTGAGCA TCCAAATGGG 
ATCATGATTG GATACACTCT CAAATATGTG GCCTTTAACG GGACCAAAGT AGGAAAGCAG 
ATAGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCACGG TGCAAAGAAC GGACCCCGTG 
TCACGCTACC GCTTTACCCT CAGCGCCAGG ACGCAGGTGG GCTCTGGGGA AGCCGTCACA 
GAGGAGTCAC CAGCACCCCC GAATGAAGCT ACTCCAACCG CAGCTCCTCC CACATTGCCC 
CCGACTACCG TGGGTGCGAC GGGCGCTGTG AGCAGTACCG ATGCTACTGC CATTGCTGCC 
ACCACCGAAG CCACAACAGT CCCCATCATC CCAACTGTCG CACCTACCAC CATCGCCACC 
ACCACCACCG TCGCCACAAC TACTACAACC ACTGCTGCCG CCACCACCAC CACGGAGAGT 
CCTCCCACCA CCACCTCCGG GACTAAGATA CACGAATCCG CCCCTGATGA GCAGTCCATA 
TGGAACGTCA CGGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC 
GGGCCCGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAACTGTC 
CCAGTTAAGG CCCAGGCTCA GCCTATACAG CTGACAGACC TCTATCCCGG GATGACATAC 
ACGTTGCGGG TTTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG 
ACCAGTACAG CTTACACCAA CAACCAAGCG GACATCGCCA CCCAGGGCTG GTTCATTGGG 
CTTATGTGCG CCATCGCCCT CCTGGTGCTG ATCCTGCTCA TCGTCTGTTT CATCAAGAGG 
AGTCGCGGCG GCAAGTACCC AGTACGAGAA AAGAAGGATG TTCCCCTTGG CCCTGAAGAC 
CCCAAGGAAG AGGATGGCTC ATTTGACTAT AGTGATGAGG ACAACAAGCC CCTGCAGGGC 
AGTCAGACAT CTCTGGACGG CACCATCAAG CAGCAGGAGA GTGACGACAG CCTGGTGGAC 
TATGGCGAGG GTGGCGAGGG TCAGTTCAAT GAAGACGGCT CCTTCATCGG CCAGTACACG 
GTCAAAAAGG ACAAGGAGGA AACAGAGGGC AACGAAAGCT CAGAGGCCAC GTCACCTGTC 
AATGCTATCT ACTCTCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509320.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507622.1 Homo sapiens neurofascin (NFASC), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu65467
Clone ID Related Accession (Same CDS sequence)	XM_011509320.2
Accession Version	XM_011509320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACACCA 
AGCTTCATGT ATCCCCAGGG CACCGCGAGC AGCCAGATGG TGCTTCGTGG CATGGACCTC 
CTGCTGGAAT GCATCGCCTC CGGGGTCCCA ACACCAGACA TCGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGATAA GGCCAAGTTT GAGAACTTTA ATAAGGCCCT GCGTATCACA 
AATGTCTCTG AGGAAGACTC CGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCCT ACTGGCTGGA CGAACCCAAG 
AACCTTATTC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGAAACCCC 
AAACCCACTG TCCAGTGGAT GGTGAATGGG GAACCTTTGC AATCGGCACC ACCTAACCCA 
AACCGTGAGG TGGCCGGAGA CACCATCATC TTCCGGGACA CCCAGATCAG CAGCAGGGCT 
GTGTACCAGT GCAACACCTC CAACGAGCAT GGCTACCTGC TGGCCAACGC CTTTGTCAGT 
GTGCTGGATG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATTCG AGTGATTCTT 
TACAACCGGA CGCGGCTGGA CTGCCCTTTC TTTGGGTCTC CCATCCCCAC ACTGCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGCAACT ACCATGTTTA TGAGAACGGC 
AGTCTGGAAA TTAAGATGAT CCGCAAAGAG GACCAGGGCA TCTACACCTG TGTCGCCACC 
AACATCCTGG GCAAAGCTGA AAACCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACCGGATGC CCGAGGACCA GGTGGCCAGA AGGGGCACCA CGGTGCAGCT GGAGTGTCGG 
GTGAAGCACG ACCCCTCCCT GAAACTCACC GTCTCCTGGC TGAAGGATGA CGAGCCGCTC 
TATATTGGAA ACAGGATGAA GAAGGAAGAC GACTCCCTGA CCATCTTTGG GGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTGTCGCC AGCACCGAGC TAGACCAAGA CCTGGCCAAG 
GCCTACCTCA CCGTGCTAGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTGCCC 
AAAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTAC TCCAACCGCA GCTCCTCCCA CATTGCCCCC GACTACCGTG 
GGTGCGACGG GCGCTGTGAG CAGTACCGAT GCTACTGCCA TTGCTGCCAC CACCGAAGCC 
ACAACAGTCC CCATCATCCC AACTGTCGCA CCTACCACCA TCGCCACCAC CACCACCGTC 
GCCACAACTA CTACAACCAC TGCTGCCGCC ACCACCACCA CGGAGAGTCC TCCCACCACC 
ACCTCCGGGA CTAAGATACA CGAATCCGCC CCTGATGAGC AGTCCATATG GAACGTCACG 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC AGTTAAGGCC 
CAGGCTCAGC CTATACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGGGTT 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAACA ACCAAGCGGA CATCGCCACC CAGGGCTGGT TCATTGGGCT TATGTGCGCC 
ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCAG TACGAGAAAA GAAGGATGTT CCCCTTGGCC CTGAAGACCC CAAGGAAGAG 
GATGGCTCAT TTGACTATAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG TCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGTC AGTTCAATGA AGACGGCTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC 
AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA GAGGCCACGT CACCTGTCAA TGCTATCTAC 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507622.1
CDS	25..4356
Translation	MRRLAARGAAGDAGEVAAPAADSSDSAQGRSPSPWRLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509320.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509320.2

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACACCA 
AGCTTCATGT ATCCCCAGGG CACCGCGAGC AGCCAGATGG TGCTTCGTGG CATGGACCTC 
CTGCTGGAAT GCATCGCCTC CGGGGTCCCA ACACCAGACA TCGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGATAA GGCCAAGTTT GAGAACTTTA ATAAGGCCCT GCGTATCACA 
AATGTCTCTG AGGAAGACTC CGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCCT ACTGGCTGGA CGAACCCAAG 
AACCTTATTC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGAAACCCC 
AAACCCACTG TCCAGTGGAT GGTGAATGGG GAACCTTTGC AATCGGCACC ACCTAACCCA 
AACCGTGAGG TGGCCGGAGA CACCATCATC TTCCGGGACA CCCAGATCAG CAGCAGGGCT 
GTGTACCAGT GCAACACCTC CAACGAGCAT GGCTACCTGC TGGCCAACGC CTTTGTCAGT 
GTGCTGGATG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATTCG AGTGATTCTT 
TACAACCGGA CGCGGCTGGA CTGCCCTTTC TTTGGGTCTC CCATCCCCAC ACTGCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGCAACT ACCATGTTTA TGAGAACGGC 
AGTCTGGAAA TTAAGATGAT CCGCAAAGAG GACCAGGGCA TCTACACCTG TGTCGCCACC 
AACATCCTGG GCAAAGCTGA AAACCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACCGGATGC CCGAGGACCA GGTGGCCAGA AGGGGCACCA CGGTGCAGCT GGAGTGTCGG 
GTGAAGCACG ACCCCTCCCT GAAACTCACC GTCTCCTGGC TGAAGGATGA CGAGCCGCTC 
TATATTGGAA ACAGGATGAA GAAGGAAGAC GACTCCCTGA CCATCTTTGG GGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTGTCGCC AGCACCGAGC TAGACCAAGA CCTGGCCAAG 
GCCTACCTCA CCGTGCTAGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTGCCC 
AAAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTAC TCCAACCGCA GCTCCTCCCA CATTGCCCCC GACTACCGTG 
GGTGCGACGG GCGCTGTGAG CAGTACCGAT GCTACTGCCA TTGCTGCCAC CACCGAAGCC 
ACAACAGTCC CCATCATCCC AACTGTCGCA CCTACCACCA TCGCCACCAC CACCACCGTC 
GCCACAACTA CTACAACCAC TGCTGCCGCC ACCACCACCA CGGAGAGTCC TCCCACCACC 
ACCTCCGGGA CTAAGATACA CGAATCCGCC CCTGATGAGC AGTCCATATG GAACGTCACG 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC AGTTAAGGCC 
CAGGCTCAGC CTATACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGGGTT 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAACA ACCAAGCGGA CATCGCCACC CAGGGCTGGT TCATTGGGCT TATGTGCGCC 
ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCAG TACGAGAAAA GAAGGATGTT CCCCTTGGCC CTGAAGACCC CAAGGAAGAG 
GATGGCTCAT TTGACTATAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG TCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGTC AGTTCAATGA AGACGGCTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC 
AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA GAGGCCACGT CACCTGTCAA TGCTATCTAC 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509322.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507624.1 Homo sapiens neurofascin (NFASC), transcript variant X15, mRNA.

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CloneID	OHu65469
Clone ID Related Accession (Same CDS sequence)	XM_011509322.1
Accession Version	XM_011509322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTGCGA CGGGCGCTGT GAGCAGTACC GATGCTACTG CCATTGCTGC CACCACCGAA 
GCCACAACAG TCCCCATCAT CCCAACTGTC GCACCTACCA CCATCGCCAC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCTGCC GCCACCACCA CCACGGAGAG TCCTCCCACC 
ACCACCTCCG GGACTAAGAT ACACGAATCC GCTTACACCA ACAACCAAGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTTATGTGC GCCATCGCCC TCCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CAGTACGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA GAGGATGGCT CATTTGACTA TAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGTCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GTCAGTTCAA TGAAGACGGC 
TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAGGAGG AAACAGAGGG CAACGAAAGC 
TCAGAGGCCA CGTCACCTGT CAATGCTATC TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507624.1
CDS	91..3915
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509322.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509322.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTGCGA CGGGCGCTGT GAGCAGTACC GATGCTACTG CCATTGCTGC CACCACCGAA 
GCCACAACAG TCCCCATCAT CCCAACTGTC GCACCTACCA CCATCGCCAC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCTGCC GCCACCACCA CCACGGAGAG TCCTCCCACC 
ACCACCTCCG GGACTAAGAT ACACGAATCC GCTTACACCA ACAACCAAGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTTATGTGC GCCATCGCCC TCCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CAGTACGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA GAGGATGGCT CATTTGACTA TAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGTCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GTCAGTTCAA TGAAGACGGC 
TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAGGAGG AAACAGAGGG CAACGAAAGC 
TCAGAGGCCA CGTCACCTGT CAATGCTATC TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509323.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507625.1 Homo sapiens neurofascin (NFASC), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu65470
Clone ID Related Accession (Same CDS sequence)	XM_011509323.1
Accession Version	XM_011509323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TTACCCAGTG CCCCTAGGCG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG 
GAATGGGATC ATCCTGAGCA TCCAAATGGG ATCATGATTG GATACACTCT CAAATATGTG 
GCCTTTAACG GGACCAAAGT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCACGG TGCAAAGAAC GGACCCCGTG TCACGCTACC GCTTTACCCT CAGCGCCAGG 
ACGCAGGTGG GCTCTGGGGA AGCCGTCACA GAGGAGTCAC CAGCACCCCC GAATGAAGCT 
ACTCCAACCG CAGCTCCTCC CACATTGCCC CCGACTACCG TGGGTGCGAC GGGCGCTGTG 
AGCAGTACCG ATGCTACTGC CATTGCTGCC ACCACCGAAG CCACAACAGT CCCCATCATC 
CCAACTGTCG CACCTACCAC CATCGCCACC ACCACCACCG TCGCCACAAC TACTACAACC 
ACTGCTGCCG CCACCACCAC CACGGAGAGT CCTCCCACCA CCACCTCCGG GACTAAGATA 
CACGAATCCG CCCCTGATGA GCAGTCCATA TGGAACGTCA CGGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC GGGCCCGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCAGTTAAGG CCCAGGCTCA GCCTATACAG 
CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGGG TTTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA CAACCAAGCG 
GACATCGCCA CCCAGGGCTG GTTCATTGGG CTTATGTGCG CCATCGCCCT CCTGGTGCTG 
ATCCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGCGGCG GCAAGTACCC AGTACGAGAA 
AAGAAGGATG TTCCCCTTGG CCCTGAAGAC CCCAAGGAAG AGGATGGCTC ATTTGACTAT 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGTCAGACAT CTCTGGACGG CACCATCAAG 
CAGCAGGAGA GTGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG TCAGTTCAAT 
GAAGACGGCT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAGGAGGA AACAGAGGGC 
AACGAAAGCT CAGAGGCCAC GTCACCTGTC AATGCTATCT ACTCTCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507625.1
CDS	91..3864
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509323.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509323.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TTACCCAGTG CCCCTAGGCG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG 
GAATGGGATC ATCCTGAGCA TCCAAATGGG ATCATGATTG GATACACTCT CAAATATGTG 
GCCTTTAACG GGACCAAAGT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCACGG TGCAAAGAAC GGACCCCGTG TCACGCTACC GCTTTACCCT CAGCGCCAGG 
ACGCAGGTGG GCTCTGGGGA AGCCGTCACA GAGGAGTCAC CAGCACCCCC GAATGAAGCT 
ACTCCAACCG CAGCTCCTCC CACATTGCCC CCGACTACCG TGGGTGCGAC GGGCGCTGTG 
AGCAGTACCG ATGCTACTGC CATTGCTGCC ACCACCGAAG CCACAACAGT CCCCATCATC 
CCAACTGTCG CACCTACCAC CATCGCCACC ACCACCACCG TCGCCACAAC TACTACAACC 
ACTGCTGCCG CCACCACCAC CACGGAGAGT CCTCCCACCA CCACCTCCGG GACTAAGATA 
CACGAATCCG CCCCTGATGA GCAGTCCATA TGGAACGTCA CGGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC GGGCCCGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCAGTTAAGG CCCAGGCTCA GCCTATACAG 
CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGGG TTTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA CAACCAAGCG 
GACATCGCCA CCCAGGGCTG GTTCATTGGG CTTATGTGCG CCATCGCCCT CCTGGTGCTG 
ATCCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGCGGCG GCAAGTACCC AGTACGAGAA 
AAGAAGGATG TTCCCCTTGG CCCTGAAGAC CCCAAGGAAG AGGATGGCTC ATTTGACTAT 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGTCAGACAT CTCTGGACGG CACCATCAAG 
CAGCAGGAGA GTGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG TCAGTTCAAT 
GAAGACGGCT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAGGAGGA AACAGAGGGC 
AACGAAAGCT CAGAGGCCAC GTCACCTGTC AATGCTATCT ACTCTCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001365986.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352915.1 Homo sapiens neurofascin (NFASC), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu107102
Clone ID Related Accession (Same CDS sequence)	NM_001365986.1
Accession Version	NM_001365986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	neurofascin isoform 7 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC096675.2 and AL391822.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.272567.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCAAC CACCCTTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTTA 
CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG CAGCCCAACC TGGAGACAAT CAACCTGGAA 
TGGGATCATC CTGAGCATCC AAATGGGATC ATGATTGGAT ACACTCTCAA ATATGTGGCC 
TTTAACGGGA CCAAAGTAGG AAAGCAGATA GTGGAAAACT TCTCTCCCAA TCAGACCAAG 
TTCACGGTGC AAAGAACGGA CCCCGTGTCA CGCTACCGCT TTACCCTCAG CGCCAGGACG 
CAGGTGGGCT CTGGGGAAGC CGTCACAGAG GAGTCACCAG CACCCCCGAA TGAAGCTTAC 
ACCAACAACC AAGCGGACAT CGCCACCCAG GGCTGGTTCA TTGGGCTTAT GTGCGCCATC 
GCCCTCCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCAGTAC GAGAAAAGAA GGATGTTCCC CTTGGCCCTG AAGACCCCAA GGAAGAGGAT 
GGCTCATTTG ACTATAGTGA TGAGGACAAC AAGCCCCTGC AGGGCAGTCA GACATCTCTG 
GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGTCAGT TCAATGAAGA CGGCTCCTTC ATCGGCCAGT ACACGGTCAA AAAGGACAAG 
GAGGAAACAG AGGGCAACGA AAGCTCAGAG GCCACGTCAC CTGTCAATGC TATCTACTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352915.1
CDS	331..3519
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001365986.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365986.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCAAC CACCCTTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTTA 
CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG CAGCCCAACC TGGAGACAAT CAACCTGGAA 
TGGGATCATC CTGAGCATCC AAATGGGATC ATGATTGGAT ACACTCTCAA ATATGTGGCC 
TTTAACGGGA CCAAAGTAGG AAAGCAGATA GTGGAAAACT TCTCTCCCAA TCAGACCAAG 
TTCACGGTGC AAAGAACGGA CCCCGTGTCA CGCTACCGCT TTACCCTCAG CGCCAGGACG 
CAGGTGGGCT CTGGGGAAGC CGTCACAGAG GAGTCACCAG CACCCCCGAA TGAAGCTTAC 
ACCAACAACC AAGCGGACAT CGCCACCCAG GGCTGGTTCA TTGGGCTTAT GTGCGCCATC 
GCCCTCCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCAGTAC GAGAAAAGAA GGATGTTCCC CTTGGCCCTG AAGACCCCAA GGAAGAGGAT 
GGCTCATTTG ACTATAGTGA TGAGGACAAC AAGCCCCTGC AGGGCAGTCA GACATCTCTG 
GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGTCAGT TCAATGAAGA CGGCTCCTTC ATCGGCCAGT ACACGGTCAA AAAGGACAAG 
GAGGAAACAG AGGGCAACGA AAGCTCAGAG GCCACGTCAC CTGTCAATGC TATCTACTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454285.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310053.1 Homo sapiens neurofascin (NFASC), transcript variant X4, mRNA.

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CloneID	OHu104314
Clone ID Related Accession (Same CDS sequence)	XM_024454285.1
Accession Version	XM_024454285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGG ACGGCCAGAC 
CGGCCCCGGG ACCTGGAGCT GACCGACCTG GCCGAGAGGA GCGTGCGGCT GACCTGGATC 
CCCGGGGATG CTAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAAGACCAG 
TTCCAACCTG GGGTCTGGCA TGACCATTCC AAGTACCCCG GCAGCGTTAA CTCAGCCGTC 
CTCCGGCTGT CCCCGTATGT CAACTACCAG TTCCGTGTCA TTGCCATCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTCCC ATCCGAGCGC TACCGAACCA GTGGAGCACC CCCCGAGTCC 
AATCCTGGTG ACGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCCATGAATG CCACCTCGGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGAGGCGG 
AGAGAGACTC GAGAGGCCTG GAACAACGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACCCCAG TCTACGTGCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTCGGGAAG 
GGCCCTGAGC CAGAGTCCGT CATCGGTTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAGTCCGAGT CATGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTACTCCAA CCGCAGCTCC TCCCACATTG CCCCCGACTA CCGTGGGTGC GACGGGCGCT 
GTGAGCAGTA CCGATGCTAC TGCCATTGCT GCCACCACCG AAGCCACAAC AGTCCCCATC 
ATCCCAACTG TCGCACCTAC CACCATCGCC ACCACCACCA CCGTCGCCAC AACTACTACA 
ACCACTGCTG CCGCCACCAC CACCACGGAG AGTCCTCCCA CCACCACCTC CGGGACTAAG 
ATACACGAAT CCGCCCCTGA TGAGCAGTCC ATATGGAACG TCACGGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCGGGCCCG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCAGTTA AGGCCCAGGC TCAGCCTATA 
CAGCTGACAG ACCTCTATCC CGGGATGACA TACACGTTGC GGGTTTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAACAACCAA 
GCGGACATCG CCACCCAGGG CTGGTTCATT GGGCTTATGT GCGCCATCGC CCTCCTGGTG 
CTGATCCTGC TCATCGTCTG TTTCATCAAG AGGAGTCGCG GCGGCAAGTA CCCAGTACGA 
GAAAAGAAGG ATGTTCCCCT TGGCCCTGAA GACCCCAAGG AAGAGGATGG CTCATTTGAC 
TATAGTGATG AGGACAACAA GCCCCTGCAG GGCAGTCAGA CATCTCTGGA CGGCACCATC 
AAGCAGCAGG AGAGTGACGA CAGCCTGGTG GACTATGGCG AGGGTGGCGA GGGTCAGTTC 
AATGAAGACG GCTCCTTCAT CGGCCAGTAC ACGGTCAAAA AGGACAAGGA GGAAACAGAG 
GGCAACGAAA GCTCAGAGGC CACGTCACCT GTCAATGCTA TCTACTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310053.1
CDS	74..4570
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024454285.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454285.1

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGG ACGGCCAGAC 
CGGCCCCGGG ACCTGGAGCT GACCGACCTG GCCGAGAGGA GCGTGCGGCT GACCTGGATC 
CCCGGGGATG CTAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAAGACCAG 
TTCCAACCTG GGGTCTGGCA TGACCATTCC AAGTACCCCG GCAGCGTTAA CTCAGCCGTC 
CTCCGGCTGT CCCCGTATGT CAACTACCAG TTCCGTGTCA TTGCCATCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTCCC ATCCGAGCGC TACCGAACCA GTGGAGCACC CCCCGAGTCC 
AATCCTGGTG ACGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCCATGAATG CCACCTCGGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGAGGCGG 
AGAGAGACTC GAGAGGCCTG GAACAACGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACCCCAG TCTACGTGCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTCGGGAAG 
GGCCCTGAGC CAGAGTCCGT CATCGGTTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAGTCCGAGT CATGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTACTCCAA CCGCAGCTCC TCCCACATTG CCCCCGACTA CCGTGGGTGC GACGGGCGCT 
GTGAGCAGTA CCGATGCTAC TGCCATTGCT GCCACCACCG AAGCCACAAC AGTCCCCATC 
ATCCCAACTG TCGCACCTAC CACCATCGCC ACCACCACCA CCGTCGCCAC AACTACTACA 
ACCACTGCTG CCGCCACCAC CACCACGGAG AGTCCTCCCA CCACCACCTC CGGGACTAAG 
ATACACGAAT CCGCCCCTGA TGAGCAGTCC ATATGGAACG TCACGGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCGGGCCCG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCAGTTA AGGCCCAGGC TCAGCCTATA 
CAGCTGACAG ACCTCTATCC CGGGATGACA TACACGTTGC GGGTTTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAACAACCAA 
GCGGACATCG CCACCCAGGG CTGGTTCATT GGGCTTATGT GCGCCATCGC CCTCCTGGTG 
CTGATCCTGC TCATCGTCTG TTTCATCAAG AGGAGTCGCG GCGGCAAGTA CCCAGTACGA 
GAAAAGAAGG ATGTTCCCCT TGGCCCTGAA GACCCCAAGG AAGAGGATGG CTCATTTGAC 
TATAGTGATG AGGACAACAA GCCCCTGCAG GGCAGTCAGA CATCTCTGGA CGGCACCATC 
AAGCAGCAGG AGAGTGACGA CAGCCTGGTG GACTATGGCG AGGGTGGCGA GGGTCAGTTC 
AATGAAGACG GCTCCTTCAT CGGCCAGTAC ACGGTCAAAA AGGACAAGGA GGAAACAGAG 
GGCAACGAAA GCTCAGAGGC CACGTCACCT GTCAATGCTA TCTACTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454288.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310056.1 Homo sapiens neurofascin (NFASC), transcript variant X5, mRNA.

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CloneID	OHu104315
Clone ID Related Accession (Same CDS sequence)	XM_024454288.1
Accession Version	XM_024454288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCACCCGAGG AGTTGCAGAA AGAACACCAA GCTTCATGTA TCCCCAGGGC 
ACCGCGAGCA GCCAGATGGT GCTTCGTGGC ATGGACCTCC TGCTGGAATG CATCGCCTCC 
GGGGTCCCAA CACCAGACAT CGCATGGTAC AAGAAAGGTG GGGACCTCCC ATCTGATAAG 
GCCAAGTTTG AGAACTTTAA TAAGGCCCTG CGTATCACAA ATGTCTCTGA GGAAGACTCC 
GGGGAGTATT TCTGCCTGGC CTCCAACAAG ATGGGCAGCA TCCGGCACAC GATCTCGGTG 
AGAGTAAAGG CTGCTCCCTA CTGGCTGGAC GAACCCAAGA ACCTTATTCT GGCTCCTGGC 
GAGGATGGGA GACTGGTGTG TCGAGCCAAT GGAAACCCCA AACCCACTGT CCAGTGGATG 
GTGAATGGGG AACCTTTGCA ATCGGCACCA CCTAACCCAA ACCGTGAGGT GGCCGGAGAC 
ACCATCATCT TCCGGGACAC CCAGATCAGC AGCAGGGCTG TGTACCAGTG CAACACCTCC 
AACGAGCATG GCTACCTGCT GGCCAACGCC TTTGTCAGTG TGCTGGATGT GCCGCCTCGG 
ATGCTGTCGC CCCGGAACCA GCTCATTCGA GTGATTCTTT ACAACCGGAC GCGGCTGGAC 
TGCCCTTTCT TTGGGTCTCC CATCCCCACA CTGCGATGGT TTAAGAATGG GCAAGGAAGC 
AACCTGGATG GTGGCAACTA CCATGTTTAT GAGAACGGCA GTCTGGAAAT TAAGATGATC 
CGCAAAGAGG ACCAGGGCAT CTACACCTGT GTCGCCACCA ACATCCTGGG CAAAGCTGAA 
AACCAAGTCC GCCTGGAGGT CAAAGACCCC ACCAGGATCT ACCGGATGCC CGAGGACCAG 
GTGGCCAGAA GGGGCACCAC GGTGCAGCTG GAGTGTCGGG TGAAGCACGA CCCCTCCCTG 
AAACTCACCG TCTCCTGGCT GAAGGATGAC GAGCCGCTCT ATATTGGAAA CAGGATGAAG 
AAGGAAGACG ACTCCCTGAC CATCTTTGGG GTGGCAGAGC GGGACCAGGG CAGTTACACG 
TGTGTCGCCA GCACCGAGCT AGACCAAGAC CTGGCCAAGG CCTACCTCAC CGTGCTAGCT 
GATCAGGCCA CTCCAACTAA CCGTTTGGCT GCCCTGCCCA AAGGACGGCC AGACCGGCCC 
CGGGACCTGG AGCTGACCGA CCTGGCCGAG AGGAGCGTGC GGCTGACCTG GATCCCCGGG 
GATGCTAACA ACAGCCCCAT CACAGACTAC GTCGTCCAGT TTGAAGAAGA CCAGTTCCAA 
CCTGGGGTCT GGCATGACCA TTCCAAGTAC CCCGGCAGCG TTAACTCAGC CGTCCTCCGG 
CTGTCCCCGT ATGTCAACTA CCAGTTCCGT GTCATTGCCA TCAACGAGGT TGGGAGCAGC 
CACCCCAGCC TCCCATCCGA GCGCTACCGA ACCAGTGGAG CACCCCCCGA GTCCAATCCT 
GGTGACGTGA AGGGAGAGGG GACCAGAAAG AACAACATGG AGATCACGTG GACGCCCATG 
AATGCCACCT CGGCCTTTGG CCCCAACCTG CGCTACATTG TCAAGTGGAG GCGGAGAGAG 
ACTCGAGAGG CCTGGAACAA CGTCACAGTG TGGGGCTCTC GCTACGTGGT GGGGCAGACC 
CCAGTCTACG TGCCCTATGA GATCCGAGTC CAGGCTGAAA ATGACTTCGG GAAGGGCCCT 
GAGCCAGAGT CCGTCATCGG TTACTCCGGA GAAGATTATC CCAGGGCTGC GCCCACTGAA 
GTTAAAGTCC GAGTCATGAA CAGCACAGCC ATCAGCCTTC AGTGGAACCG CGTCTACTCC 
GACACGGTCC AGGGCCAGCT CAGAGAGTAC CGAGCCTACT ACTGGAGGGA GAGCAGCTTG 
CTGAAGAACC TGTGGGTGTC TCAGAAGAGA CAGCAAGCCA GCTTCCCTGG TGACCGCCTC 
CGTGGCGTGG TGTCCCGCCT CTTCCCCTAC AGTAACTACA AGCTGGAGAT GGTTGTGGTC 
AATGGGAGAG GTGATGGGCC TCGCAGTGAG ACCAAGGAGT TCACCACCCC GGAAGGAGTA 
CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG CAGCCCAACC TGGAGACAAT CAACCTGGAA 
TGGGATCATC CTGAGCATCC AAATGGGATC ATGATTGGAT ACACTCTCAA ATATGTGGCC 
TTTAACGGGA CCAAAGTAGG AAAGCAGATA GTGGAAAACT TCTCTCCCAA TCAGACCAAG 
TTCACGGTGC AAAGAACGGA CCCCGTGTCA CGCTACCGCT TTACCCTCAG CGCCAGGACG 
CAGGTGGGCT CTGGGGAAGC CGTCACAGAG GAGTCACCAG CACCCCCGAA TGAAGCTACT 
CCAACCGCAG CTCCTCCCAC ATTGCCCCCG ACTACCGTGG GTGCGACGGG CGCTGTGAGC 
AGTACCGATG CTACTGCCAT TGCTGCCACC ACCGAAGCCA CAACAGTCCC CATCATCCCA 
ACTGTCGCAC CTACCACCAT CGCCACCACC ACCACCGTCG CCACAACTAC TACAACCACT 
GCTGCCGCCA CCACCACCAC GGAGAGTCCT CCCACCACCA CCTCCGGGAC TAAGATACAC 
GAATCCGCCC CTGATGAGCA GTCCATATGG AACGTCACGG TGCTCCCCAA CAGTAAATGG 
GCCAACATCA CCTGGAAGCA CAATTTCGGG CCCGGAACTG ACTTTGTGGT TGAGTACATC 
GACAGCAACC ATACGAAAAA AACTGTCCCA GTTAAGGCCC AGGCTCAGCC TATACAGCTG 
ACAGACCTCT ATCCCGGGAT GACATACACG TTGCGGGTTT ATTCCCGGGA CAACGAGGGC 
ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT ACACCAACAA CCAAGCGGAC 
ATCGCCACCC AGGGCTGGTT CATTGGGCTT ATGTGCGCCA TCGCCCTCCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCAGT ACGAGAAAAG 
AAGGATGTTC CCCTTGGCCC TGAAGACCCC AAGGAAGAGG ATGGCTCATT TGACTATAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGT CAGACATCTC TGGACGGCAC CATCAAGCAG 
CAGGAGAGTG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGTCA GTTCAATGAA 
GACGGCTCCT TCATCGGCCA GTACACGGTC AAAAAGGACA AGGAGGAAAC AGAGGGCAAC 
GAAAGCTCAG AGGCCACGTC ACCTGTCAAT GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310056.1
CDS	72..4562
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024454288.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454288.1

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCACCCGAGG AGTTGCAGAA AGAACACCAA GCTTCATGTA TCCCCAGGGC 
ACCGCGAGCA GCCAGATGGT GCTTCGTGGC ATGGACCTCC TGCTGGAATG CATCGCCTCC 
GGGGTCCCAA CACCAGACAT CGCATGGTAC AAGAAAGGTG GGGACCTCCC ATCTGATAAG 
GCCAAGTTTG AGAACTTTAA TAAGGCCCTG CGTATCACAA ATGTCTCTGA GGAAGACTCC 
GGGGAGTATT TCTGCCTGGC CTCCAACAAG ATGGGCAGCA TCCGGCACAC GATCTCGGTG 
AGAGTAAAGG CTGCTCCCTA CTGGCTGGAC GAACCCAAGA ACCTTATTCT GGCTCCTGGC 
GAGGATGGGA GACTGGTGTG TCGAGCCAAT GGAAACCCCA AACCCACTGT CCAGTGGATG 
GTGAATGGGG AACCTTTGCA ATCGGCACCA CCTAACCCAA ACCGTGAGGT GGCCGGAGAC 
ACCATCATCT TCCGGGACAC CCAGATCAGC AGCAGGGCTG TGTACCAGTG CAACACCTCC 
AACGAGCATG GCTACCTGCT GGCCAACGCC TTTGTCAGTG TGCTGGATGT GCCGCCTCGG 
ATGCTGTCGC CCCGGAACCA GCTCATTCGA GTGATTCTTT ACAACCGGAC GCGGCTGGAC 
TGCCCTTTCT TTGGGTCTCC CATCCCCACA CTGCGATGGT TTAAGAATGG GCAAGGAAGC 
AACCTGGATG GTGGCAACTA CCATGTTTAT GAGAACGGCA GTCTGGAAAT TAAGATGATC 
CGCAAAGAGG ACCAGGGCAT CTACACCTGT GTCGCCACCA ACATCCTGGG CAAAGCTGAA 
AACCAAGTCC GCCTGGAGGT CAAAGACCCC ACCAGGATCT ACCGGATGCC CGAGGACCAG 
GTGGCCAGAA GGGGCACCAC GGTGCAGCTG GAGTGTCGGG TGAAGCACGA CCCCTCCCTG 
AAACTCACCG TCTCCTGGCT GAAGGATGAC GAGCCGCTCT ATATTGGAAA CAGGATGAAG 
AAGGAAGACG ACTCCCTGAC CATCTTTGGG GTGGCAGAGC GGGACCAGGG CAGTTACACG 
TGTGTCGCCA GCACCGAGCT AGACCAAGAC CTGGCCAAGG CCTACCTCAC CGTGCTAGCT 
GATCAGGCCA CTCCAACTAA CCGTTTGGCT GCCCTGCCCA AAGGACGGCC AGACCGGCCC 
CGGGACCTGG AGCTGACCGA CCTGGCCGAG AGGAGCGTGC GGCTGACCTG GATCCCCGGG 
GATGCTAACA ACAGCCCCAT CACAGACTAC GTCGTCCAGT TTGAAGAAGA CCAGTTCCAA 
CCTGGGGTCT GGCATGACCA TTCCAAGTAC CCCGGCAGCG TTAACTCAGC CGTCCTCCGG 
CTGTCCCCGT ATGTCAACTA CCAGTTCCGT GTCATTGCCA TCAACGAGGT TGGGAGCAGC 
CACCCCAGCC TCCCATCCGA GCGCTACCGA ACCAGTGGAG CACCCCCCGA GTCCAATCCT 
GGTGACGTGA AGGGAGAGGG GACCAGAAAG AACAACATGG AGATCACGTG GACGCCCATG 
AATGCCACCT CGGCCTTTGG CCCCAACCTG CGCTACATTG TCAAGTGGAG GCGGAGAGAG 
ACTCGAGAGG CCTGGAACAA CGTCACAGTG TGGGGCTCTC GCTACGTGGT GGGGCAGACC 
CCAGTCTACG TGCCCTATGA GATCCGAGTC CAGGCTGAAA ATGACTTCGG GAAGGGCCCT 
GAGCCAGAGT CCGTCATCGG TTACTCCGGA GAAGATTATC CCAGGGCTGC GCCCACTGAA 
GTTAAAGTCC GAGTCATGAA CAGCACAGCC ATCAGCCTTC AGTGGAACCG CGTCTACTCC 
GACACGGTCC AGGGCCAGCT CAGAGAGTAC CGAGCCTACT ACTGGAGGGA GAGCAGCTTG 
CTGAAGAACC TGTGGGTGTC TCAGAAGAGA CAGCAAGCCA GCTTCCCTGG TGACCGCCTC 
CGTGGCGTGG TGTCCCGCCT CTTCCCCTAC AGTAACTACA AGCTGGAGAT GGTTGTGGTC 
AATGGGAGAG GTGATGGGCC TCGCAGTGAG ACCAAGGAGT TCACCACCCC GGAAGGAGTA 
CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG CAGCCCAACC TGGAGACAAT CAACCTGGAA 
TGGGATCATC CTGAGCATCC AAATGGGATC ATGATTGGAT ACACTCTCAA ATATGTGGCC 
TTTAACGGGA CCAAAGTAGG AAAGCAGATA GTGGAAAACT TCTCTCCCAA TCAGACCAAG 
TTCACGGTGC AAAGAACGGA CCCCGTGTCA CGCTACCGCT TTACCCTCAG CGCCAGGACG 
CAGGTGGGCT CTGGGGAAGC CGTCACAGAG GAGTCACCAG CACCCCCGAA TGAAGCTACT 
CCAACCGCAG CTCCTCCCAC ATTGCCCCCG ACTACCGTGG GTGCGACGGG CGCTGTGAGC 
AGTACCGATG CTACTGCCAT TGCTGCCACC ACCGAAGCCA CAACAGTCCC CATCATCCCA 
ACTGTCGCAC CTACCACCAT CGCCACCACC ACCACCGTCG CCACAACTAC TACAACCACT 
GCTGCCGCCA CCACCACCAC GGAGAGTCCT CCCACCACCA CCTCCGGGAC TAAGATACAC 
GAATCCGCCC CTGATGAGCA GTCCATATGG AACGTCACGG TGCTCCCCAA CAGTAAATGG 
GCCAACATCA CCTGGAAGCA CAATTTCGGG CCCGGAACTG ACTTTGTGGT TGAGTACATC 
GACAGCAACC ATACGAAAAA AACTGTCCCA GTTAAGGCCC AGGCTCAGCC TATACAGCTG 
ACAGACCTCT ATCCCGGGAT GACATACACG TTGCGGGTTT ATTCCCGGGA CAACGAGGGC 
ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT ACACCAACAA CCAAGCGGAC 
ATCGCCACCC AGGGCTGGTT CATTGGGCTT ATGTGCGCCA TCGCCCTCCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCAGT ACGAGAAAAG 
AAGGATGTTC CCCTTGGCCC TGAAGACCCC AAGGAAGAGG ATGGCTCATT TGACTATAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGT CAGACATCTC TGGACGGCAC CATCAAGCAG 
CAGGAGAGTG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGTCA GTTCAATGAA 
GACGGCTCCT TCATCGGCCA GTACACGGTC AAAAAGGACA AGGAGGAAAC AGAGGGCAAC 
GAAAGCTCAG AGGCCACGTC ACCTGTCAAT GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454292.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310060.1 Homo sapiens neurofascin (NFASC), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu104316
Clone ID Related Accession (Same CDS sequence)	XM_024454292.1
Accession Version	XM_024454292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCACCCGAGG AGTTGCAGAA AGAACACCAA GCTTCATGTA TCCCCAGGGC 
ACCGCGAGCA GCCAGATGGT GCTTCGTGGC ATGGACCTCC TGCTGGAATG CATCGCCTCC 
GGGGTCCCAA CACCAGACAT CGCATGGTAC AAGAAAGGTG GGGACCTCCC ATCTGATAAG 
GCCAAGTTTG AGAACTTTAA TAAGGCCCTG CGTATCACAA ATGTCTCTGA GGAAGACTCC 
GGGGAGTATT TCTGCCTGGC CTCCAACAAG ATGGGCAGCA TCCGGCACAC GATCTCGGTG 
AGAGTAAAGG CTGCTCCCTA CTGGCTGGAC GAACCCAAGA ACCTTATTCT GGCTCCTGGC 
GAGGATGGGA GACTGGTGTG TCGAGCCAAT GGAAACCCCA AACCCACTGT CCAGTGGATG 
GTGAATGGGG AACCTTTGCA ATCGGCACCA CCTAACCCAA ACCGTGAGGT GGCCGGAGAC 
ACCATCATCT TCCGGGACAC CCAGATCAGC AGCAGGGCTG TGTACCAGTG CAACACCTCC 
AACGAGCATG GCTACCTGCT GGCCAACGCC TTTGTCAGTG TGCTGGATGT GCCGCCTCGG 
ATGCTGTCGC CCCGGAACCA GCTCATTCGA GTGATTCTTT ACAACCGGAC GCGGCTGGAC 
TGCCCTTTCT TTGGGTCTCC CATCCCCACA CTGCGATGGT TTAAGAATGG GCAAGGAAGC 
AACCTGGATG GTGGCAACTA CCATGTTTAT GAGAACGGCA GTCTGGAAAT TAAGATGATC 
CGCAAAGAGG ACCAGGGCAT CTACACCTGT GTCGCCACCA ACATCCTGGG CAAAGCTGAA 
AACCAAGTCC GCCTGGAGGT CAAAGACCCC ACCAGGATCT ACCGGATGCC CGAGGACCAG 
GTGGCCAGAA GGGGCACCAC GGTGCAGCTG GAGTGTCGGG TGAAGCACGA CCCCTCCCTG 
AAACTCACCG TCTCCTGGCT GAAGGATGAC GAGCCGCTCT ATATTGGAAA CAGGATGAAG 
AAGGAAGACG ACTCCCTGAC CATCTTTGGG GTGGCAGAGC GGGACCAGGG CAGTTACACG 
TGTGTCGCCA GCACCGAGCT AGACCAAGAC CTGGCCAAGG CCTACCTCAC CGTGCTAGGA 
CGGCCAGACC GGCCCCGGGA CCTGGAGCTG ACCGACCTGG CCGAGAGGAG CGTGCGGCTG 
ACCTGGATCC CCGGGGATGC TAACAACAGC CCCATCACAG ACTACGTCGT CCAGTTTGAA 
GAAGACCAGT TCCAACCTGG GGTCTGGCAT GACCATTCCA AGTACCCCGG CAGCGTTAAC 
TCAGCCGTCC TCCGGCTGTC CCCGTATGTC AACTACCAGT TCCGTGTCAT TGCCATCAAC 
GAGGTTGGGA GCAGCCACCC CAGCCTCCCA TCCGAGCGCT ACCGAACCAG TGGAGCACCC 
CCCGAGTCCA ATCCTGGTGA CGTGAAGGGA GAGGGGACCA GAAAGAACAA CATGGAGATC 
ACGTGGACGC CCATGAATGC CACCTCGGCC TTTGGCCCCA ACCTGCGCTA CATTGTCAAG 
TGGAGGCGGA GAGAGACTCG AGAGGCCTGG AACAACGTCA CAGTGTGGGG CTCTCGCTAC 
GTGGTGGGGC AGACCCCAGT CTACGTGCCC TATGAGATCC GAGTCCAGGC TGAAAATGAC 
TTCGGGAAGG GCCCTGAGCC AGAGTCCGTC ATCGGTTACT CCGGAGAAGA TTATCCCAGG 
GCTGCGCCCA CTGAAGTTAA AGTCCGAGTC ATGAACAGCA CAGCCATCAG CCTTCAGTGG 
AACCGCGTCT ACTCCGACAC GGTCCAGGGC CAGCTCAGAG AGTACCGAGC CTACTACTGG 
AGGGAGAGCA GCTTGCTGAA GAACCTGTGG GTGTCTCAGA AGAGACAGCA AGCCAGCTTC 
CCTGGTGACC GCCTCCGTGG CGTGGTGTCC CGCCTCTTCC CCTACAGTAA CTACAAGCTG 
GAGATGGTTG TGGTCAATGG GAGAGGTGAT GGGCCTCGCA GTGAGACCAA GGAGTTCACC 
ACCCCGGAAG GAGTACCCAG TGCCCCTAGG CGTTTCCGAG TCCGGCAGCC CAACCTGGAG 
ACAATCAACC TGGAATGGGA TCATCCTGAG CATCCAAATG GGATCATGAT TGGATACACT 
CTCAAATATG TGGCCTTTAA CGGGACCAAA GTAGGAAAGC AGATAGTGGA AAACTTCTCT 
CCCAATCAGA CCAAGTTCAC GGTGCAAAGA ACGGACCCCG TGTCACGCTA CCGCTTTACC 
CTCAGCGCCA GGACGCAGGT GGGCTCTGGG GAAGCCGTCA CAGAGGAGTC ACCAGCACCC 
CCGAATGAAG CTACTCCAAC CGCAGCTCCT CCCACATTGC CCCCGACTAC CGTGGGTGCG 
ACGGGCGCTG TGAGCAGTAC CGATGCTACT GCCATTGCTG CCACCACCGA AGCCACAACA 
GTCCCCATCA TCCCAACTGT CGCACCTACC ACCATCGCCA CCACCACCAC CGTCGCCACA 
ACTACTACAA CCACTGCTGC CGCCACCACC ACCACGGAGA GTCCTCCCAC CACCACCTCC 
GGGACTAAGA TACACGAATC CGCCCCTGAT GAGCAGTCCA TATGGAACGT CACGGTGCTC 
CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT 
GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAACTG TCCCAGTTAA GGCCCAGGCT 
CAGCCTATAC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG GGTTTATTCC 
CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC 
AACAACCAAG CGGACATCGC CACCCAGGGC TGGTTCATTG GGCTTATGTG CGCCATCGCC 
CTCCTGGTGC TGATCCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGCGG CGGCAAGTAC 
CCAGTACGAG AAAAGAAGGA TGTTCCCCTT GGCCCTGAAG ACCCCAAGGA AGAGGATGGC 
TCATTTGACT ATAGTGATGA GGACAACAAG CCCCTGCAGG GCAGTCAGAC ATCTCTGGAC 
GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTATGGCGA GGGTGGCGAG 
GGTCAGTTCA ATGAAGACGG CTCCTTCATC GGCCAGTACA CGGTCAAAAA GGACAAGGAG 
GAAACAGAGG GCAACGAAAG CTCAGAGGCC ACGTCACCTG TCAATGCTAT CTACTCTCTG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310060.1
CDS	73..4518
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024454292.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454292.1

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCACCCGAGG AGTTGCAGAA AGAACACCAA GCTTCATGTA TCCCCAGGGC 
ACCGCGAGCA GCCAGATGGT GCTTCGTGGC ATGGACCTCC TGCTGGAATG CATCGCCTCC 
GGGGTCCCAA CACCAGACAT CGCATGGTAC AAGAAAGGTG GGGACCTCCC ATCTGATAAG 
GCCAAGTTTG AGAACTTTAA TAAGGCCCTG CGTATCACAA ATGTCTCTGA GGAAGACTCC 
GGGGAGTATT TCTGCCTGGC CTCCAACAAG ATGGGCAGCA TCCGGCACAC GATCTCGGTG 
AGAGTAAAGG CTGCTCCCTA CTGGCTGGAC GAACCCAAGA ACCTTATTCT GGCTCCTGGC 
GAGGATGGGA GACTGGTGTG TCGAGCCAAT GGAAACCCCA AACCCACTGT CCAGTGGATG 
GTGAATGGGG AACCTTTGCA ATCGGCACCA CCTAACCCAA ACCGTGAGGT GGCCGGAGAC 
ACCATCATCT TCCGGGACAC CCAGATCAGC AGCAGGGCTG TGTACCAGTG CAACACCTCC 
AACGAGCATG GCTACCTGCT GGCCAACGCC TTTGTCAGTG TGCTGGATGT GCCGCCTCGG 
ATGCTGTCGC CCCGGAACCA GCTCATTCGA GTGATTCTTT ACAACCGGAC GCGGCTGGAC 
TGCCCTTTCT TTGGGTCTCC CATCCCCACA CTGCGATGGT TTAAGAATGG GCAAGGAAGC 
AACCTGGATG GTGGCAACTA CCATGTTTAT GAGAACGGCA GTCTGGAAAT TAAGATGATC 
CGCAAAGAGG ACCAGGGCAT CTACACCTGT GTCGCCACCA ACATCCTGGG CAAAGCTGAA 
AACCAAGTCC GCCTGGAGGT CAAAGACCCC ACCAGGATCT ACCGGATGCC CGAGGACCAG 
GTGGCCAGAA GGGGCACCAC GGTGCAGCTG GAGTGTCGGG TGAAGCACGA CCCCTCCCTG 
AAACTCACCG TCTCCTGGCT GAAGGATGAC GAGCCGCTCT ATATTGGAAA CAGGATGAAG 
AAGGAAGACG ACTCCCTGAC CATCTTTGGG GTGGCAGAGC GGGACCAGGG CAGTTACACG 
TGTGTCGCCA GCACCGAGCT AGACCAAGAC CTGGCCAAGG CCTACCTCAC CGTGCTAGGA 
CGGCCAGACC GGCCCCGGGA CCTGGAGCTG ACCGACCTGG CCGAGAGGAG CGTGCGGCTG 
ACCTGGATCC CCGGGGATGC TAACAACAGC CCCATCACAG ACTACGTCGT CCAGTTTGAA 
GAAGACCAGT TCCAACCTGG GGTCTGGCAT GACCATTCCA AGTACCCCGG CAGCGTTAAC 
TCAGCCGTCC TCCGGCTGTC CCCGTATGTC AACTACCAGT TCCGTGTCAT TGCCATCAAC 
GAGGTTGGGA GCAGCCACCC CAGCCTCCCA TCCGAGCGCT ACCGAACCAG TGGAGCACCC 
CCCGAGTCCA ATCCTGGTGA CGTGAAGGGA GAGGGGACCA GAAAGAACAA CATGGAGATC 
ACGTGGACGC CCATGAATGC CACCTCGGCC TTTGGCCCCA ACCTGCGCTA CATTGTCAAG 
TGGAGGCGGA GAGAGACTCG AGAGGCCTGG AACAACGTCA CAGTGTGGGG CTCTCGCTAC 
GTGGTGGGGC AGACCCCAGT CTACGTGCCC TATGAGATCC GAGTCCAGGC TGAAAATGAC 
TTCGGGAAGG GCCCTGAGCC AGAGTCCGTC ATCGGTTACT CCGGAGAAGA TTATCCCAGG 
GCTGCGCCCA CTGAAGTTAA AGTCCGAGTC ATGAACAGCA CAGCCATCAG CCTTCAGTGG 
AACCGCGTCT ACTCCGACAC GGTCCAGGGC CAGCTCAGAG AGTACCGAGC CTACTACTGG 
AGGGAGAGCA GCTTGCTGAA GAACCTGTGG GTGTCTCAGA AGAGACAGCA AGCCAGCTTC 
CCTGGTGACC GCCTCCGTGG CGTGGTGTCC CGCCTCTTCC CCTACAGTAA CTACAAGCTG 
GAGATGGTTG TGGTCAATGG GAGAGGTGAT GGGCCTCGCA GTGAGACCAA GGAGTTCACC 
ACCCCGGAAG GAGTACCCAG TGCCCCTAGG CGTTTCCGAG TCCGGCAGCC CAACCTGGAG 
ACAATCAACC TGGAATGGGA TCATCCTGAG CATCCAAATG GGATCATGAT TGGATACACT 
CTCAAATATG TGGCCTTTAA CGGGACCAAA GTAGGAAAGC AGATAGTGGA AAACTTCTCT 
CCCAATCAGA CCAAGTTCAC GGTGCAAAGA ACGGACCCCG TGTCACGCTA CCGCTTTACC 
CTCAGCGCCA GGACGCAGGT GGGCTCTGGG GAAGCCGTCA CAGAGGAGTC ACCAGCACCC 
CCGAATGAAG CTACTCCAAC CGCAGCTCCT CCCACATTGC CCCCGACTAC CGTGGGTGCG 
ACGGGCGCTG TGAGCAGTAC CGATGCTACT GCCATTGCTG CCACCACCGA AGCCACAACA 
GTCCCCATCA TCCCAACTGT CGCACCTACC ACCATCGCCA CCACCACCAC CGTCGCCACA 
ACTACTACAA CCACTGCTGC CGCCACCACC ACCACGGAGA GTCCTCCCAC CACCACCTCC 
GGGACTAAGA TACACGAATC CGCCCCTGAT GAGCAGTCCA TATGGAACGT CACGGTGCTC 
CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT 
GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAACTG TCCCAGTTAA GGCCCAGGCT 
CAGCCTATAC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG GGTTTATTCC 
CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC 
AACAACCAAG CGGACATCGC CACCCAGGGC TGGTTCATTG GGCTTATGTG CGCCATCGCC 
CTCCTGGTGC TGATCCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGCGG CGGCAAGTAC 
CCAGTACGAG AAAAGAAGGA TGTTCCCCTT GGCCCTGAAG ACCCCAAGGA AGAGGATGGC 
TCATTTGACT ATAGTGATGA GGACAACAAG CCCCTGCAGG GCAGTCAGAC ATCTCTGGAC 
GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTATGGCGA GGGTGGCGAG 
GGTCAGTTCA ATGAAGACGG CTCCTTCATC GGCCAGTACA CGGTCAAAAA GGACAAGGAG 
GAAACAGAGG GCAACGAAAG CTCAGAGGCC ACGTCACCTG TCAATGCTAT CTACTCTCTG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509321.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507623.2 Homo sapiens neurofascin (NFASC), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu104317
Clone ID Related Accession (Same CDS sequence)	XM_011509321.2
Accession Version	XM_011509321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTAC TCCAACCGCA 
GCCCCTGATG AGCAGTCCAT ATGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC 
ATCACCTGGA AGCACAATTT CGGGCCCGGA ACTGACTTTG TGGTTGAGTA CATCGACAGC 
AACCATACGA AAAAAACTGT CCCAGTTAAG GCCCAGGCTC AGCCTATACA GCTGACAGAC 
CTCTATCCCG GGATGACATA CACGTTGCGG GTTTATTCCC GGGACAACGA GGGCATCAGC 
AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ACAACCAAGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTTATGTGC GCCATCGCCC TCCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CAGTACGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA GAGGATGGCT CATTTGACTA TAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGTCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GTCAGTTCAA TGAAGACGGC 
TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAGGAGG AAACAGAGGG CAACGAAAGC 
TCAGAGGCCA CGTCACCTGT CAATGCTATC TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507623.2
CDS	72..4376
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509321.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509321.2

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTAC TCCAACCGCA 
GCCCCTGATG AGCAGTCCAT ATGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC 
ATCACCTGGA AGCACAATTT CGGGCCCGGA ACTGACTTTG TGGTTGAGTA CATCGACAGC 
AACCATACGA AAAAAACTGT CCCAGTTAAG GCCCAGGCTC AGCCTATACA GCTGACAGAC 
CTCTATCCCG GGATGACATA CACGTTGCGG GTTTATTCCC GGGACAACGA GGGCATCAGC 
AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ACAACCAAGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTTATGTGC GCCATCGCCC TCCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CAGTACGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA GAGGATGGCT CATTTGACTA TAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGTCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GTCAGTTCAA TGAAGACGGC 
TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAGGAGG AAACAGAGGG CAACGAAAGC 
TCAGAGGCCA CGTCACCTGT CAATGCTATC TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509322.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507624.2 Homo sapiens neurofascin (NFASC), transcript variant X15, mRNA.

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CloneID	OHu104318
Clone ID Related Accession (Same CDS sequence)	XM_011509322.2
Accession Version	XM_011509322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTAC TCCAACCGCA 
GCTCCTCCCA CATTGCCCCC GACTACCGTG GGTGCGACGG GCGCTGTGAG CAGTACCGAT 
GCTACTGCCA TTGCTGCCAC CACCGAAGCC ACAACAGTCC CCATCATCCC AACTGTCGCA 
CCTACCACCA TCGCCACCAC CACCACCGTC GCCACAACTA CTACAACCAC TGCTGCCGCC 
ACCACCACCA CGGAGAGTCC TCCCACCACC ACCTCCGGGA CTAAGATACA CGAATCCGCT 
TACACCAACA ACCAAGCGGA CATCGCCACC CAGGGCTGGT TCATTGGGCT TATGTGCGCC 
ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCAG TACGAGAAAA GAAGGATGTT CCCCTTGGCC CTGAAGACCC CAAGGAAGAG 
GATGGCTCAT TTGACTATAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG TCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGTC AGTTCAATGA AGACGGCTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC 
AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA GAGGCCACGT CACCTGTCAA TGCTATCTAC 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507624.2
CDS	72..4343
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509322.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509322.2

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTAC TCCAACCGCA 
GCTCCTCCCA CATTGCCCCC GACTACCGTG GGTGCGACGG GCGCTGTGAG CAGTACCGAT 
GCTACTGCCA TTGCTGCCAC CACCGAAGCC ACAACAGTCC CCATCATCCC AACTGTCGCA 
CCTACCACCA TCGCCACCAC CACCACCGTC GCCACAACTA CTACAACCAC TGCTGCCGCC 
ACCACCACCA CGGAGAGTCC TCCCACCACC ACCTCCGGGA CTAAGATACA CGAATCCGCT 
TACACCAACA ACCAAGCGGA CATCGCCACC CAGGGCTGGT TCATTGGGCT TATGTGCGCC 
ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCAG TACGAGAAAA GAAGGATGTT CCCCTTGGCC CTGAAGACCC CAAGGAAGAG 
GATGGCTCAT TTGACTATAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG TCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGTC AGTTCAATGA AGACGGCTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC 
AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA GAGGCCACGT CACCTGTCAA TGCTATCTAC 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509323.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507625.2 Homo sapiens neurofascin (NFASC), transcript variant X16, mRNA.

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CloneID	OHu104319
Clone ID Related Accession (Same CDS sequence)	XM_011509323.2
Accession Version	XM_011509323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTTA CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CTGAGCATCC AAATGGGATC 
ATGATTGGAT ACACTCTCAA ATATGTGGCC TTTAACGGGA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAACGGA CCCCGTGTCA 
CGCTACCGCT TTACCCTCAG CGCCAGGACG CAGGTGGGCT CTGGGGAAGC CGTCACAGAG 
GAGTCACCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC ATTGCCCCCG 
ACTACCGTGG GTGCGACGGG CGCTGTGAGC AGTACCGATG CTACTGCCAT TGCTGCCACC 
ACCGAAGCCA CAACAGTCCC CATCATCCCA ACTGTCGCAC CTACCACCAT CGCCACCACC 
ACCACCGTCG CCACAACTAC TACAACCACT GCTGCCGCCA CCACCACCAC GGAGAGTCCT 
CCCACCACCA CCTCCGGGAC TAAGATACAC GAATCCGCCC CTGATGAGCA GTCCATATGG 
AACGTCACGG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCGGG 
CCCGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCA 
GTTAAGGCCC AGGCTCAGCC TATACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG 
TTGCGGGTTT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAACAA CCAAGCGGAC ATCGCCACCC AGGGCTGGTT CATTGGGCTT 
ATGTGCGCCA TCGCCCTCCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCAGT ACGAGAAAAG AAGGATGTTC CCCTTGGCCC TGAAGACCCC 
AAGGAAGAGG ATGGCTCATT TGACTATAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGT 
CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGTCA GTTCAATGAA GACGGCTCCT TCATCGGCCA GTACACGGTC 
AAAAAGGACA AGGAGGAAAC AGAGGGCAAC GAAAGCTCAG AGGCCACGTC ACCTGTCAAT 
GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507625.2
CDS	69..4289
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509323.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509323.2

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTTA CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CTGAGCATCC AAATGGGATC 
ATGATTGGAT ACACTCTCAA ATATGTGGCC TTTAACGGGA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAACGGA CCCCGTGTCA 
CGCTACCGCT TTACCCTCAG CGCCAGGACG CAGGTGGGCT CTGGGGAAGC CGTCACAGAG 
GAGTCACCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC ATTGCCCCCG 
ACTACCGTGG GTGCGACGGG CGCTGTGAGC AGTACCGATG CTACTGCCAT TGCTGCCACC 
ACCGAAGCCA CAACAGTCCC CATCATCCCA ACTGTCGCAC CTACCACCAT CGCCACCACC 
ACCACCGTCG CCACAACTAC TACAACCACT GCTGCCGCCA CCACCACCAC GGAGAGTCCT 
CCCACCACCA CCTCCGGGAC TAAGATACAC GAATCCGCCC CTGATGAGCA GTCCATATGG 
AACGTCACGG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCGGG 
CCCGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCA 
GTTAAGGCCC AGGCTCAGCC TATACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG 
TTGCGGGTTT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAACAA CCAAGCGGAC ATCGCCACCC AGGGCTGGTT CATTGGGCTT 
ATGTGCGCCA TCGCCCTCCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCAGT ACGAGAAAAG AAGGATGTTC CCCTTGGCCC TGAAGACCCC 
AAGGAAGAGG ATGGCTCATT TGACTATAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGT 
CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGTCA GTTCAATGAA GACGGCTCCT TCATCGGCCA GTACACGGTC 
AAAAAGGACA AGGAGGAAAC AGAGGGCAAC GAAAGCTCAG AGGCCACGTC ACCTGTCAAT 
GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454296.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310064.1 Homo sapiens neurofascin (NFASC), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu104320
Clone ID Related Accession (Same CDS sequence)	XM_024454296.1
Accession Version	XM_024454296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCACCCGAGG AGTTGCAGAA AGAACACCAA GCTTCATGTA TCCCCAGGGC 
ACCGCGAGCA GCCAGATGGT GCTTCGTGGC ATGGACCTCC TGCTGGAATG CATCGCCTCC 
GGGGTCCCAA CACCAGACAT CGCATGGTAC AAGAAAGGTG GGGACCTCCC ATCTGATAAG 
GCCAAGTTTG AGAACTTTAA TAAGGCCCTG CGTATCACAA ATGTCTCTGA GGAAGACTCC 
GGGGAGTATT TCTGCCTGGC CTCCAACAAG ATGGGCAGCA TCCGGCACAC GATCTCGGTG 
AGAGTAAAGG CTGCTCCCTA CTGGCTGGAC GAACCCAAGA ACCTTATTCT GGCTCCTGGC 
GAGGATGGGA GACTGGTGTG TCGAGCCAAT GGAAACCCCA AACCCACTGT CCAGTGGATG 
GTGAATGGGG AACCTTTGCA ATCGGCACCA CCTAACCCAA ACCGTGAGGT GGCCGGAGAC 
ACCATCATCT TCCGGGACAC CCAGATCAGC AGCAGGGCTG TGTACCAGTG CAACACCTCC 
AACGAGCATG GCTACCTGCT GGCCAACGCC TTTGTCAGTG TGCTGGATGT GCCGCCTCGG 
ATGCTGTCGC CCCGGAACCA GCTCATTCGA GTGATTCTTT ACAACCGGAC GCGGCTGGAC 
TGCCCTTTCT TTGGGTCTCC CATCCCCACA CTGCGATGGT TTAAGAATGG GCAAGGAAGC 
AACCTGGATG GTGGCAACTA CCATGTTTAT GAGAACGGCA GTCTGGAAAT TAAGATGATC 
CGCAAAGAGG ACCAGGGCAT CTACACCTGT GTCGCCACCA ACATCCTGGG CAAAGCTGAA 
AACCAAGTCC GCCTGGAGGT CAAAGACCCC ACCAGGATCT ACCGGATGCC CGAGGACCAG 
GTGGCCAGAA GGGGCACCAC GGTGCAGCTG GAGTGTCGGG TGAAGCACGA CCCCTCCCTG 
AAACTCACCG TCTCCTGGCT GAAGGATGAC GAGCCGCTCT ATATTGGAAA CAGGATGAAG 
AAGGAAGACG ACTCCCTGAC CATCTTTGGG GTGGCAGAGC GGGACCAGGG CAGTTACACG 
TGTGTCGCCA GCACCGAGCT AGACCAAGAC CTGGCCAAGG CCTACCTCAC CGTGCTAGCT 
GATCAGGCCA CTCCAACTAA CCGTTTGGCT GCCCTGCCCA AAGGACGGCC AGACCGGCCC 
CGGGACCTGG AGCTGACCGA CCTGGCCGAG AGGAGCGTGC GGCTGACCTG GATCCCCGGG 
GATGCTAACA ACAGCCCCAT CACAGACTAC GTCGTCCAGT TTGAAGAAGA CCAGTTCCAA 
CCTGGGGTCT GGCATGACCA TTCCAAGTAC CCCGGCAGCG TTAACTCAGC CGTCCTCCGG 
CTGTCCCCGT ATGTCAACTA CCAGTTCCGT GTCATTGCCA TCAACGAGGT TGGGAGCAGC 
CACCCCAGCC TCCCATCCGA GCGCTACCGA ACCAGTGGAG CACCCCCCGA GTCCAATCCT 
GGTGACGTGA AGGGAGAGGG GACCAGAAAG AACAACATGG AGATCACGTG GACGCCCATG 
AATGCCACCT CGGCCTTTGG CCCCAACCTG CGCTACATTG TCAAGTGGAG GCGGAGAGAG 
ACTCGAGAGG CCTGGAACAA CGTCACAGTG TGGGGCTCTC GCTACGTGGT GGGGCAGACC 
CCAGTCTACG TGCCCTATGA GATCCGAGTC CAGGCTGAAA ATGACTTCGG GAAGGGCCCT 
GAGCCAGAGT CCGTCATCGG TTACTCCGGA GAAGATTTAC CCAGTGCCCC TAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA 
AATGGGATCA TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC 
CCCGTGTCAC GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC 
GTCACAGAGG AGTCACCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TGCGACGGGC GCTGTGAGCA GTACCGATGC TACTGCCATT 
GCTGCCACCA CCGAAGCCAC AACAGTCCCC ATCATCCCAA CTGTCGCACC TACCACCATC 
GCCACCACCA CCACCGTCGC CACAACTACT ACAACCACTG CTGCCGCCAC CACCACCACG 
GAGAGTCCTC CCACCACCAC CTCCGGGACT AAGATACACG AATCCGCCCC TGATGAGCAG 
TCCATATGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCAG TTAAGGCCCA GGCTCAGCCT ATACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGGGTTTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310064.1
CDS	67..4236
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024454296.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454296.1

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCACCCGAGG AGTTGCAGAA AGAACACCAA GCTTCATGTA TCCCCAGGGC 
ACCGCGAGCA GCCAGATGGT GCTTCGTGGC ATGGACCTCC TGCTGGAATG CATCGCCTCC 
GGGGTCCCAA CACCAGACAT CGCATGGTAC AAGAAAGGTG GGGACCTCCC ATCTGATAAG 
GCCAAGTTTG AGAACTTTAA TAAGGCCCTG CGTATCACAA ATGTCTCTGA GGAAGACTCC 
GGGGAGTATT TCTGCCTGGC CTCCAACAAG ATGGGCAGCA TCCGGCACAC GATCTCGGTG 
AGAGTAAAGG CTGCTCCCTA CTGGCTGGAC GAACCCAAGA ACCTTATTCT GGCTCCTGGC 
GAGGATGGGA GACTGGTGTG TCGAGCCAAT GGAAACCCCA AACCCACTGT CCAGTGGATG 
GTGAATGGGG AACCTTTGCA ATCGGCACCA CCTAACCCAA ACCGTGAGGT GGCCGGAGAC 
ACCATCATCT TCCGGGACAC CCAGATCAGC AGCAGGGCTG TGTACCAGTG CAACACCTCC 
AACGAGCATG GCTACCTGCT GGCCAACGCC TTTGTCAGTG TGCTGGATGT GCCGCCTCGG 
ATGCTGTCGC CCCGGAACCA GCTCATTCGA GTGATTCTTT ACAACCGGAC GCGGCTGGAC 
TGCCCTTTCT TTGGGTCTCC CATCCCCACA CTGCGATGGT TTAAGAATGG GCAAGGAAGC 
AACCTGGATG GTGGCAACTA CCATGTTTAT GAGAACGGCA GTCTGGAAAT TAAGATGATC 
CGCAAAGAGG ACCAGGGCAT CTACACCTGT GTCGCCACCA ACATCCTGGG CAAAGCTGAA 
AACCAAGTCC GCCTGGAGGT CAAAGACCCC ACCAGGATCT ACCGGATGCC CGAGGACCAG 
GTGGCCAGAA GGGGCACCAC GGTGCAGCTG GAGTGTCGGG TGAAGCACGA CCCCTCCCTG 
AAACTCACCG TCTCCTGGCT GAAGGATGAC GAGCCGCTCT ATATTGGAAA CAGGATGAAG 
AAGGAAGACG ACTCCCTGAC CATCTTTGGG GTGGCAGAGC GGGACCAGGG CAGTTACACG 
TGTGTCGCCA GCACCGAGCT AGACCAAGAC CTGGCCAAGG CCTACCTCAC CGTGCTAGCT 
GATCAGGCCA CTCCAACTAA CCGTTTGGCT GCCCTGCCCA AAGGACGGCC AGACCGGCCC 
CGGGACCTGG AGCTGACCGA CCTGGCCGAG AGGAGCGTGC GGCTGACCTG GATCCCCGGG 
GATGCTAACA ACAGCCCCAT CACAGACTAC GTCGTCCAGT TTGAAGAAGA CCAGTTCCAA 
CCTGGGGTCT GGCATGACCA TTCCAAGTAC CCCGGCAGCG TTAACTCAGC CGTCCTCCGG 
CTGTCCCCGT ATGTCAACTA CCAGTTCCGT GTCATTGCCA TCAACGAGGT TGGGAGCAGC 
CACCCCAGCC TCCCATCCGA GCGCTACCGA ACCAGTGGAG CACCCCCCGA GTCCAATCCT 
GGTGACGTGA AGGGAGAGGG GACCAGAAAG AACAACATGG AGATCACGTG GACGCCCATG 
AATGCCACCT CGGCCTTTGG CCCCAACCTG CGCTACATTG TCAAGTGGAG GCGGAGAGAG 
ACTCGAGAGG CCTGGAACAA CGTCACAGTG TGGGGCTCTC GCTACGTGGT GGGGCAGACC 
CCAGTCTACG TGCCCTATGA GATCCGAGTC CAGGCTGAAA ATGACTTCGG GAAGGGCCCT 
GAGCCAGAGT CCGTCATCGG TTACTCCGGA GAAGATTTAC CCAGTGCCCC TAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA 
AATGGGATCA TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC 
CCCGTGTCAC GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC 
GTCACAGAGG AGTCACCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TGCGACGGGC GCTGTGAGCA GTACCGATGC TACTGCCATT 
GCTGCCACCA CCGAAGCCAC AACAGTCCCC ATCATCCCAA CTGTCGCACC TACCACCATC 
GCCACCACCA CCACCGTCGC CACAACTACT ACAACCACTG CTGCCGCCAC CACCACCACG 
GAGAGTCCTC CCACCACCAC CTCCGGGACT AAGATACACG AATCCGCCCC TGATGAGCAG 
TCCATATGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCAG TTAAGGCCCA GGCTCAGCCT ATACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGGGTTTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454297.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310065.1 Homo sapiens neurofascin (NFASC), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu104321
Clone ID Related Accession (Same CDS sequence)	XM_024454297.1
Accession Version	XM_024454297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTAC TCCAACCGCA 
GCTTACACCA ACAACCAAGC GGACATCGCC ACCCAGGGCT GGTTCATTGG GCTTATGTGC 
GCCATCGCCC TCCTGGTGCT GATCCTGCTC ATCGTCTGTT TCATCAAGAG GAGTCGCGGC 
GGCAAGTACC CAGTACGAGA AAAGAAGGAT GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA 
GAGGATGGCT CATTTGACTA TAGTGATGAG GACAACAAGC CCCTGCAGGG CAGTCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTGGTGGA CTATGGCGAG 
GGTGGCGAGG GTCAGTTCAA TGAAGACGGC TCCTTCATCG GCCAGTACAC GGTCAAAAAG 
GACAAGGAGG AAACAGAGGG CAACGAAAGC TCAGAGGCCA CGTCACCTGT CAATGCTATC 
TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310065.1
CDS	69..4103
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024454297.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454297.1

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTAC TCCAACCGCA 
GCTTACACCA ACAACCAAGC GGACATCGCC ACCCAGGGCT GGTTCATTGG GCTTATGTGC 
GCCATCGCCC TCCTGGTGCT GATCCTGCTC ATCGTCTGTT TCATCAAGAG GAGTCGCGGC 
GGCAAGTACC CAGTACGAGA AAAGAAGGAT GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA 
GAGGATGGCT CATTTGACTA TAGTGATGAG GACAACAAGC CCCTGCAGGG CAGTCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTGGTGGA CTATGGCGAG 
GGTGGCGAGG GTCAGTTCAA TGAAGACGGC TCCTTCATCG GCCAGTACAC GGTCAAAAAG 
GACAAGGAGG AAACAGAGGG CAACGAAAGC TCAGAGGCCA CGTCACCTGT CAATGCTATC 
TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509325.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507627.2 Homo sapiens neurofascin (NFASC), transcript variant X19, mRNA.

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CloneID	OHu104322
Clone ID Related Accession (Same CDS sequence)	XM_011509325.2
Accession Version	XM_011509325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTTA CACCAACAAC 
CAAGCGGACA TCGCCACCCA GGGCTGGTTC ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCAGTA 
CGAGAAAAGA AGGATGTTCC CCTTGGCCCT GAAGACCCCA AGGAAGAGGA TGGCTCATTT 
GACTATAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGTC AGACATCTCT GGACGGCACC 
ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGTCAG 
TTCAATGAAG ACGGCTCCTT CATCGGCCAG TACACGGTCA AAAAGGACAA GGAGGAAACA 
GAGGGCAACG AAAGCTCAGA GGCCACGTCA CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507627.2
CDS	69..4088
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509325.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509325.2

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTTA CACCAACAAC 
CAAGCGGACA TCGCCACCCA GGGCTGGTTC ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCAGTA 
CGAGAAAAGA AGGATGTTCC CCTTGGCCCT GAAGACCCCA AGGAAGAGGA TGGCTCATTT 
GACTATAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGTC AGACATCTCT GGACGGCACC 
ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGTCAG 
TTCAATGAAG ACGGCTCCTT CATCGGCCAG TACACGGTCA AAAAGGACAA GGAGGAAACA 
GAGGGCAACG AAAGCTCAGA GGCCACGTCA CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454299.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310067.1 Homo sapiens neurofascin (NFASC), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu104323
Clone ID Related Accession (Same CDS sequence)	XM_024454299.1
Accession Version	XM_024454299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGG ACGGCCAGAC 
CGGCCCCGGG ACCTGGAGCT GACCGACCTG GCCGAGAGGA GCGTGCGGCT GACCTGGATC 
CCCGGGGATG CTAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAAGACCAG 
TTCCAACCTG GGGTCTGGCA TGACCATTCC AAGTACCCCG GCAGCGTTAA CTCAGCCGTC 
CTCCGGCTGT CCCCGTATGT CAACTACCAG TTCCGTGTCA TTGCCATCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTCCC ATCCGAGCGC TACCGAACCA GTGGAGCACC CCCCGAGTCC 
AATCCTGGTG ACGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCCATGAATG CCACCTCGGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGAGGCGG 
AGAGAGACTC GAGAGGCCTG GAACAACGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACCCCAG TCTACGTGCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTCGGGAAG 
GGCCCTGAGC CAGAGTCCGT CATCGGTTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAGTCCGAGT CATGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTACTCCAA CCGCAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310067.1
CDS	70..4059
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024454299.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454299.1

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGG ACGGCCAGAC 
CGGCCCCGGG ACCTGGAGCT GACCGACCTG GCCGAGAGGA GCGTGCGGCT GACCTGGATC 
CCCGGGGATG CTAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAAGACCAG 
TTCCAACCTG GGGTCTGGCA TGACCATTCC AAGTACCCCG GCAGCGTTAA CTCAGCCGTC 
CTCCGGCTGT CCCCGTATGT CAACTACCAG TTCCGTGTCA TTGCCATCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTCCC ATCCGAGCGC TACCGAACCA GTGGAGCACC CCCCGAGTCC 
AATCCTGGTG ACGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCCATGAATG CCACCTCGGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGAGGCGG 
AGAGAGACTC GAGAGGCCTG GAACAACGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACCCCAG TCTACGTGCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTCGGGAAG 
GGCCCTGAGC CAGAGTCCGT CATCGGTTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAGTCCGAGT CATGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTACTCCAA CCGCAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509326.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507628.2 Homo sapiens neurofascin (NFASC), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OHu104324
Clone ID Related Accession (Same CDS sequence)	XM_011509326.2
Accession Version	XM_011509326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTTA CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CTGAGCATCC AAATGGGATC 
ATGATTGGAT ACACTCTCAA ATATGTGGCC TTTAACGGGA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAACGGA CCCCGTGTCA 
CGCTACCGCT TTACCCTCAG CGCCAGGACG CAGGTGGGCT CTGGGGAAGC CGTCACAGAG 
GAGTCACCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTTACACCAA CAACCAAGCG 
GACATCGCCA CCCAGGGCTG GTTCATTGGG CTTATGTGCG CCATCGCCCT CCTGGTGCTG 
ATCCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGCGGCG GCAAGTACCC AGTACGAGAA 
AAGAAGGATG TTCCCCTTGG CCCTGAAGAC CCCAAGGAAG AGGATGGCTC ATTTGACTAT 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGTCAGACAT CTCTGGACGG CACCATCAAG 
CAGCAGGAGA GTGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG TCAGTTCAAT 
GAAGACGGCT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAGGAGGA AACAGAGGGC 
AACGAAAGCT CAGAGGCCAC GTCACCTGTC AATGCTATCT ACTCTCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507628.2
CDS	64..3777
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509326.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509326.2

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTTA CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CTGAGCATCC AAATGGGATC 
ATGATTGGAT ACACTCTCAA ATATGTGGCC TTTAACGGGA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAACGGA CCCCGTGTCA 
CGCTACCGCT TTACCCTCAG CGCCAGGACG CAGGTGGGCT CTGGGGAAGC CGTCACAGAG 
GAGTCACCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTTACACCAA CAACCAAGCG 
GACATCGCCA CCCAGGGCTG GTTCATTGGG CTTATGTGCG CCATCGCCCT CCTGGTGCTG 
ATCCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGCGGCG GCAAGTACCC AGTACGAGAA 
AAGAAGGATG TTCCCCTTGG CCCTGAAGAC CCCAAGGAAG AGGATGGCTC ATTTGACTAT 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGTCAGACAT CTCTGGACGG CACCATCAAG 
CAGCAGGAGA GTGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG TCAGTTCAAT 
GAAGACGGCT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAGGAGGA AACAGAGGGC 
AACGAAAGCT CAGAGGCCAC GTCACCTGTC AATGCTATCT ACTCTCTGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509327.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507629.2 Homo sapiens neurofascin (NFASC), transcript variant X29, mRNA.

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CloneID	OHu104325
Clone ID Related Accession (Same CDS sequence)	XM_011509327.2
Accession Version	XM_011509327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTTA CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CTGAGCATCC AAATGGGATC 
ATGATTGGAT ACACTCTCAA ATATGTGGCC TTTAACGGGA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAACGGA CCCCGTGTCA 
CGCTACCGCT TTACCCTCAG CGCCAGGACG CAGGTGGGCT CTGGGGAAGC CGTCACAGAG 
GAGTCACCAG CACCCCCGAA TGAAGCTTAC ACCAACAACC AAGCGGACAT CGCCACCCAG 
GGCTGGTTCA TTGGGCTTAT GTGCGCCATC GCCCTCCTGG TGCTGATCCT GCTCATCGTC 
TGTTTCATCA AGAGGAGTCG CGGCGGCAAG TACCCAGTAC GAGAAAAGAA GGATGTTCCC 
CTTGGCCCTG AAGACCCCAA GGAAGAGGAT GGCTCATTTG ACTATAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGTCA GACATCTCTG GACGGCACCA TCAAGCAGCA GGAGAGTGAC 
GACAGCCTGG TGGACTATGG CGAGGGTGGC GAGGGTCAGT TCAATGAAGA CGGCTCCTTC 
ATCGGCCAGT ACACGGTCAA AAAGGACAAG GAGGAAACAG AGGGCAACGA AAGCTCAGAG 
GCCACGTCAC CTGTCAATGC TATCTACTCT CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507629.2
CDS	64..3762
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509327.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509327.2

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTTA CCCAGTGCCC CTAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CTGAGCATCC AAATGGGATC 
ATGATTGGAT ACACTCTCAA ATATGTGGCC TTTAACGGGA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAACGGA CCCCGTGTCA 
CGCTACCGCT TTACCCTCAG CGCCAGGACG CAGGTGGGCT CTGGGGAAGC CGTCACAGAG 
GAGTCACCAG CACCCCCGAA TGAAGCTTAC ACCAACAACC AAGCGGACAT CGCCACCCAG 
GGCTGGTTCA TTGGGCTTAT GTGCGCCATC GCCCTCCTGG TGCTGATCCT GCTCATCGTC 
TGTTTCATCA AGAGGAGTCG CGGCGGCAAG TACCCAGTAC GAGAAAAGAA GGATGTTCCC 
CTTGGCCCTG AAGACCCCAA GGAAGAGGAT GGCTCATTTG ACTATAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGTCA GACATCTCTG GACGGCACCA TCAAGCAGCA GGAGAGTGAC 
GACAGCCTGG TGGACTATGG CGAGGGTGGC GAGGGTCAGT TCAATGAAGA CGGCTCCTTC 
ATCGGCCAGT ACACGGTCAA AAAGGACAAG GAGGAAACAG AGGGCAACGA AAGCTCAGAG 
GCCACGTCAC CTGTCAATGC TATCTACTCT CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509328.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507630.2 Homo sapiens neurofascin (NFASC), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OHu104327
Clone ID Related Accession (Same CDS sequence)	XM_011509328.2
Accession Version	XM_011509328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCACCCGAGG AGTTGCAGAA AGAACACCAA GCTTCATGTA TCCCCAGGGC 
ACCGCGAGCA GCCAGATGGT GCTTCGTGGC ATGGACCTCC TGCTGGAATG CATCGCCTCC 
GGGGTCCCAA CACCAGACAT CGCATGGTAC AAGAAAGGTG GGGACCTCCC ATCTGATAAG 
GCCAAGTTTG AGAACTTTAA TAAGGCCCTG CGTATCACAA ATGTCTCTGA GGAAGACTCC 
GGGGAGTATT TCTGCCTGGC CTCCAACAAG ATGGGCAGCA TCCGGCACAC GATCTCGGTG 
AGAGTAAAGG CTGCTCCCTA CTGGCTGGAC GAACCCAAGA ACCTTATTCT GGCTCCTGGC 
GAGGATGGGA GACTGGTGTG TCGAGCCAAT GGAAACCCCA AACCCACTGT CCAGTGGATG 
GTGAATGGGG AACCTTTGCA ATCGGCACCA CCTAACCCAA ACCGTGAGGT GGCCGGAGAC 
ACCATCATCT TCCGGGACAC CCAGATCAGC AGCAGGGCTG TGTACCAGTG CAACACCTCC 
AACGAGCATG GCTACCTGCT GGCCAACGCC TTTGTCAGTG TGCTGGATGT GCCGCCTCGG 
ATGCTGTCGC CCCGGAACCA GCTCATTCGA GTGATTCTTT ACAACCGGAC GCGGCTGGAC 
TGCCCTTTCT TTGGGTCTCC CATCCCCACA CTGCGATGGT TTAAGAATGG GCAAGGAAGC 
AACCTGGATG GTGGCAACTA CCATGTTTAT GAGAACGGCA GTCTGGAAAT TAAGATGATC 
CGCAAAGAGG ACCAGGGCAT CTACACCTGT GTCGCCACCA ACATCCTGGG CAAAGCTGAA 
AACCAAGTCC GCCTGGAGGT CAAAGACCCC ACCAGGATCT ACCGGATGCC CGAGGACCAG 
GTGGCCAGAA GGGGCACCAC GGTGCAGCTG GAGTGTCGGG TGAAGCACGA CCCCTCCCTG 
AAACTCACCG TCTCCTGGCT GAAGGATGAC GAGCCGCTCT ATATTGGAAA CAGGATGAAG 
AAGGAAGACG ACTCCCTGAC CATCTTTGGG GTGGCAGAGC GGGACCAGGG CAGTTACACG 
TGTGTCGCCA GCACCGAGCT AGACCAAGAC CTGGCCAAGG CCTACCTCAC CGTGCTAGCT 
GATCAGGCCA CTCCAACTAA CCGTTTGGCT GCCCTGCCCA AAGGACGGCC AGACCGGCCC 
CGGGACCTGG AGCTGACCGA CCTGGCCGAG AGGAGCGTGC GGCTGACCTG GATCCCCGGG 
GATGCTAACA ACAGCCCCAT CACAGACTAC GTCGTCCAGT TTGAAGAAGA CCAGTTCCAA 
CCTGGGGTCT GGCATGACCA TTCCAAGTAC CCCGGCAGCG TTAACTCAGC CGTCCTCCGG 
CTGTCCCCGT ATGTCAACTA CCAGTTCCGT GTCATTGCCA TCAACGAGGT TGGGAGCAGC 
CACCCCAGCC TCCCATCCGA GCGCTACCGA ACCAGTGGAG CACCCCCCGA GTCCAATCCT 
GGTGACGTGA AGGGAGAGGG GACCAGAAAG AACAACATGG AGATCACGTG GACGCCCATG 
AATGCCACCT CGGCCTTTGG CCCCAACCTG CGCTACATTG TCAAGTGGAG GCGGAGAGAG 
ACTCGAGAGG CCTGGAACAA CGTCACAGTG TGGGGCTCTC GCTACGTGGT GGGGCAGACC 
CCAGTCTACG TGCCCTATGA GATCCGAGTC CAGGCTGAAA ATGACTTCGG GAAGGGCCCT 
GAGCCAGAGT CCGTCATCGG TTACTCCGGA GAAGATTTAC CCAGTGCCCC TAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA 
AATGGGATCA TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC 
CCCGTGTCAC GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC 
GTCACAGAGG AGTCACCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507630.2
CDS	62..3724
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509328.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509328.2

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCACCCGAGG AGTTGCAGAA AGAACACCAA GCTTCATGTA TCCCCAGGGC 
ACCGCGAGCA GCCAGATGGT GCTTCGTGGC ATGGACCTCC TGCTGGAATG CATCGCCTCC 
GGGGTCCCAA CACCAGACAT CGCATGGTAC AAGAAAGGTG GGGACCTCCC ATCTGATAAG 
GCCAAGTTTG AGAACTTTAA TAAGGCCCTG CGTATCACAA ATGTCTCTGA GGAAGACTCC 
GGGGAGTATT TCTGCCTGGC CTCCAACAAG ATGGGCAGCA TCCGGCACAC GATCTCGGTG 
AGAGTAAAGG CTGCTCCCTA CTGGCTGGAC GAACCCAAGA ACCTTATTCT GGCTCCTGGC 
GAGGATGGGA GACTGGTGTG TCGAGCCAAT GGAAACCCCA AACCCACTGT CCAGTGGATG 
GTGAATGGGG AACCTTTGCA ATCGGCACCA CCTAACCCAA ACCGTGAGGT GGCCGGAGAC 
ACCATCATCT TCCGGGACAC CCAGATCAGC AGCAGGGCTG TGTACCAGTG CAACACCTCC 
AACGAGCATG GCTACCTGCT GGCCAACGCC TTTGTCAGTG TGCTGGATGT GCCGCCTCGG 
ATGCTGTCGC CCCGGAACCA GCTCATTCGA GTGATTCTTT ACAACCGGAC GCGGCTGGAC 
TGCCCTTTCT TTGGGTCTCC CATCCCCACA CTGCGATGGT TTAAGAATGG GCAAGGAAGC 
AACCTGGATG GTGGCAACTA CCATGTTTAT GAGAACGGCA GTCTGGAAAT TAAGATGATC 
CGCAAAGAGG ACCAGGGCAT CTACACCTGT GTCGCCACCA ACATCCTGGG CAAAGCTGAA 
AACCAAGTCC GCCTGGAGGT CAAAGACCCC ACCAGGATCT ACCGGATGCC CGAGGACCAG 
GTGGCCAGAA GGGGCACCAC GGTGCAGCTG GAGTGTCGGG TGAAGCACGA CCCCTCCCTG 
AAACTCACCG TCTCCTGGCT GAAGGATGAC GAGCCGCTCT ATATTGGAAA CAGGATGAAG 
AAGGAAGACG ACTCCCTGAC CATCTTTGGG GTGGCAGAGC GGGACCAGGG CAGTTACACG 
TGTGTCGCCA GCACCGAGCT AGACCAAGAC CTGGCCAAGG CCTACCTCAC CGTGCTAGCT 
GATCAGGCCA CTCCAACTAA CCGTTTGGCT GCCCTGCCCA AAGGACGGCC AGACCGGCCC 
CGGGACCTGG AGCTGACCGA CCTGGCCGAG AGGAGCGTGC GGCTGACCTG GATCCCCGGG 
GATGCTAACA ACAGCCCCAT CACAGACTAC GTCGTCCAGT TTGAAGAAGA CCAGTTCCAA 
CCTGGGGTCT GGCATGACCA TTCCAAGTAC CCCGGCAGCG TTAACTCAGC CGTCCTCCGG 
CTGTCCCCGT ATGTCAACTA CCAGTTCCGT GTCATTGCCA TCAACGAGGT TGGGAGCAGC 
CACCCCAGCC TCCCATCCGA GCGCTACCGA ACCAGTGGAG CACCCCCCGA GTCCAATCCT 
GGTGACGTGA AGGGAGAGGG GACCAGAAAG AACAACATGG AGATCACGTG GACGCCCATG 
AATGCCACCT CGGCCTTTGG CCCCAACCTG CGCTACATTG TCAAGTGGAG GCGGAGAGAG 
ACTCGAGAGG CCTGGAACAA CGTCACAGTG TGGGGCTCTC GCTACGTGGT GGGGCAGACC 
CCAGTCTACG TGCCCTATGA GATCCGAGTC CAGGCTGAAA ATGACTTCGG GAAGGGCCCT 
GAGCCAGAGT CCGTCATCGG TTACTCCGGA GAAGATTTAC CCAGTGCCCC TAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA 
AATGGGATCA TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC 
CCCGTGTCAC GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC 
GTCACAGAGG AGTCACCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000733.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856222.1 Homo sapiens neurofascin (NFASC), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu87051
Clone ID Related Accession (Same CDS sequence)	XM_017000733.1
Accession Version	XM_017000733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGGACGG 
CCAGACCGGC CCCGGGACCT GGAGCTGACC GACCTGGCCG AGAGGAGCGT GCGGCTGACC 
TGGATCCCCG GGGATGCTAA CAACAGCCCC ATCACAGACT ACGTCGTCCA GTTTGAAGAA 
GACCAGTTCC AACCTGGGGT CTGGCATGAC CATTCCAAGT ACCCCGGCAG CGTTAACTCA 
GCCGTCCTCC GGCTGTCCCC GTATGTCAAC TACCAGTTCC GTGTCATTGC CATCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTCCCATCC GAGCGCTACC GAACCAGTGG AGCACCCCCC 
GAGTCCAATC CTGGTGACGT GAAGGGAGAG GGGACCAGAA AGAACAACAT GGAGATCACG 
TGGACGCCCA TGAATGCCAC CTCGGCCTTT GGCCCCAACC TGCGCTACAT TGTCAAGTGG 
AGGCGGAGAG AGACTCGAGA GGCCTGGAAC AACGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CCCCAGTCTA CGTGCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTC 
GGGAAGGGCC CTGAGCCAGA GTCCGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAGT CCGAGTCATG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGCGTCTACT CCGACACGGT CCAGGGCCAG CTCAGAGAGT ACCGAGCCTA CTACTGGAGG 
GAGAGCAGCT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAAGC CAGCTTCCCT 
GGTGACCGCC TCCGTGGCGT GGTGTCCCGC CTCTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTTGTGG TCAATGGGAG AGGTGATGGG CCTCGCAGTG AGACCAAGGA GTTCACCACC 
CCGGAAGGAG TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTGCGACG 
GGCGCTGTGA GCAGTACCGA TGCTACTGCC ATTGCTGCCA CCACCGAAGC CACAACAGTC 
CCCATCATCC CAACTGTCGC ACCTACCACC ATCGCCACCA CCACCACCGT CGCCACAACT 
ACTACAACCA CTGCTGCCGC CACCACCACC ACGGAGAGTC CTCCCACCAC CACCTCCGGG 
ACTAAGATAC ACGAATCCGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856222.1
CDS	178..4440
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000733.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000733.1

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGGACGG 
CCAGACCGGC CCCGGGACCT GGAGCTGACC GACCTGGCCG AGAGGAGCGT GCGGCTGACC 
TGGATCCCCG GGGATGCTAA CAACAGCCCC ATCACAGACT ACGTCGTCCA GTTTGAAGAA 
GACCAGTTCC AACCTGGGGT CTGGCATGAC CATTCCAAGT ACCCCGGCAG CGTTAACTCA 
GCCGTCCTCC GGCTGTCCCC GTATGTCAAC TACCAGTTCC GTGTCATTGC CATCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTCCCATCC GAGCGCTACC GAACCAGTGG AGCACCCCCC 
GAGTCCAATC CTGGTGACGT GAAGGGAGAG GGGACCAGAA AGAACAACAT GGAGATCACG 
TGGACGCCCA TGAATGCCAC CTCGGCCTTT GGCCCCAACC TGCGCTACAT TGTCAAGTGG 
AGGCGGAGAG AGACTCGAGA GGCCTGGAAC AACGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CCCCAGTCTA CGTGCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTC 
GGGAAGGGCC CTGAGCCAGA GTCCGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAGT CCGAGTCATG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGCGTCTACT CCGACACGGT CCAGGGCCAG CTCAGAGAGT ACCGAGCCTA CTACTGGAGG 
GAGAGCAGCT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAAGC CAGCTTCCCT 
GGTGACCGCC TCCGTGGCGT GGTGTCCCGC CTCTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTTGTGG TCAATGGGAG AGGTGATGGG CCTCGCAGTG AGACCAAGGA GTTCACCACC 
CCGGAAGGAG TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTGCGACG 
GGCGCTGTGA GCAGTACCGA TGCTACTGCC ATTGCTGCCA CCACCGAAGC CACAACAGTC 
CCCATCATCC CAACTGTCGC ACCTACCACC ATCGCCACCA CCACCACCGT CGCCACAACT 
ACTACAACCA CTGCTGCCGC CACCACCACC ACGGAGAGTC CTCCCACCAC CACCTCCGGG 
ACTAAGATAC ACGAATCCGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000734.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856223.1 Homo sapiens neurofascin (NFASC), transcript variant X9, mRNA.

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CloneID	OHu87052
Clone ID Related Accession (Same CDS sequence)	XM_017000734.1
Accession Version	XM_017000734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTAC TCCAACCGCA GCTCCTCCCA CATTGCCCCC GACTACCGTG 
GGTGCGACGG GCGCTGTGAG CAGTACCGAT GCTACTGCCA TTGCTGCCAC CACCGAAGCC 
ACAACAGTCC CCATCATCCC AACTGTCGCA CCTACCACCA TCGCCACCAC CACCACCGTC 
GCCACAACTA CTACAACCAC TGCTGCCGCC ACCACCACCA CGGAGAGTCC TCCCACCACC 
ACCTCCGGGA CTAAGATACA CGAATCCGCC CCTGATGAGC AGTCCATATG GAACGTCACG 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC AGTTAAGGCC 
CAGGCTCAGC CTATACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGGGTT 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAACA ACCAAGCGGA CATCGCCACC CAGGGCTGGT TCATTGGGCT TATGTGCGCC 
ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCAG TACGAGAAAA GAAGGATGTT CCCCTTGGCC CTGAAGACCC CAAGGAAGAG 
GATGGCTCAT TTGACTATAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG TCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGTC AGTTCAATGA AGACGGCTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC 
AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA GAGGCCACGT CACCTGTCAA TGCTATCTAC 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856223.1
CDS	178..4389
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000734.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000734.1

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTAC TCCAACCGCA GCTCCTCCCA CATTGCCCCC GACTACCGTG 
GGTGCGACGG GCGCTGTGAG CAGTACCGAT GCTACTGCCA TTGCTGCCAC CACCGAAGCC 
ACAACAGTCC CCATCATCCC AACTGTCGCA CCTACCACCA TCGCCACCAC CACCACCGTC 
GCCACAACTA CTACAACCAC TGCTGCCGCC ACCACCACCA CGGAGAGTCC TCCCACCACC 
ACCTCCGGGA CTAAGATACA CGAATCCGCC CCTGATGAGC AGTCCATATG GAACGTCACG 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC AGTTAAGGCC 
CAGGCTCAGC CTATACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGGGTT 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAACA ACCAAGCGGA CATCGCCACC CAGGGCTGGT TCATTGGGCT TATGTGCGCC 
ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCAG TACGAGAAAA GAAGGATGTT CCCCTTGGCC CTGAAGACCC CAAGGAAGAG 
GATGGCTCAT TTGACTATAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG TCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGTC AGTTCAATGA AGACGGCTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC 
AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA GAGGCCACGT CACCTGTCAA TGCTATCTAC 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000735.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856224.1 Homo sapiens neurofascin (NFASC), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu87053
Clone ID Related Accession (Same CDS sequence)	XM_017000735.1
Accession Version	XM_017000735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856224.1
CDS	91..3933
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000735.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000735.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC CCCTGATGAG CAGTCCATAT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CAGTTAAGGC CCAGGCTCAG 
CCTATACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGGGT TTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000736.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856225.1 Homo sapiens neurofascin (NFASC), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu87054
Clone ID Related Accession (Same CDS sequence)	XM_017000736.1
Accession Version	XM_017000736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGGACGGCCA GACCGGCCCC GGGACCTGGA GCTGACCGAC 
CTGGCCGAGA GGAGCGTGCG GCTGACCTGG ATCCCCGGGG ATGCTAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAAC CTGGGGTCTG GCATGACCAT 
TCCAAGTACC CCGGCAGCGT TAACTCAGCC GTCCTCCGGC TGTCCCCGTA TGTCAACTAC 
CAGTTCCGTG TCATTGCCAT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT CCCATCCGAG 
CGCTACCGAA CCAGTGGAGC ACCCCCCGAG TCCAATCCTG GTGACGTGAA GGGAGAGGGG 
ACCAGAAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC GGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGAGG CGGAGAGAGA CTCGAGAGGC CTGGAACAAC 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACCC CAGTCTACGT GCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTCGGG AAGGGCCCTG AGCCAGAGTC CGTCATCGGT 
TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAGTCCG AGTCATGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAGTACC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAAGCCAG CTTCCCTGGT GACCGCCTCC GTGGCGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGCAGTGAGA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC TAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA 
AATGGGATCA TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC 
CCCGTGTCAC GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC 
GTCACAGAGG AGTCACCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856225.1
CDS	91..3633
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000736.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000736.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGGACGGCCA GACCGGCCCC GGGACCTGGA GCTGACCGAC 
CTGGCCGAGA GGAGCGTGCG GCTGACCTGG ATCCCCGGGG ATGCTAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAAC CTGGGGTCTG GCATGACCAT 
TCCAAGTACC CCGGCAGCGT TAACTCAGCC GTCCTCCGGC TGTCCCCGTA TGTCAACTAC 
CAGTTCCGTG TCATTGCCAT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT CCCATCCGAG 
CGCTACCGAA CCAGTGGAGC ACCCCCCGAG TCCAATCCTG GTGACGTGAA GGGAGAGGGG 
ACCAGAAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC GGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGAGG CGGAGAGAGA CTCGAGAGGC CTGGAACAAC 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACCC CAGTCTACGT GCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTCGGG AAGGGCCCTG AGCCAGAGTC CGTCATCGGT 
TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAGTCCG AGTCATGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAGTACC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAAGCCAG CTTCCCTGGT GACCGCCTCC GTGGCGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGCAGTGAGA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC TAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA 
AATGGGATCA TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC 
CCCGTGTCAC GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC 
GTCACAGAGG AGTCACCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TTACACCAAC 
AACCAAGCGG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TTATGTGCGC CATCGCCCTC 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCA 
GTACGAGAAA AGAAGGATGT TCCCCTTGGC CCTGAAGACC CCAAGGAAGA GGATGGCTCA 
TTTGACTATA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GTCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGT 
CAGTTCAATG AAGACGGCTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAGGAGGAA 
ACAGAGGGCA ACGAAAGCTC AGAGGCCACG TCACCTGTCA ATGCTATCTA CTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000737.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856226.1 Homo sapiens neurofascin (NFASC), transcript variant X24, mRNA.

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CloneID	OHu87055
Clone ID Related Accession (Same CDS sequence)	XM_017000737.1
Accession Version	XM_017000737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTGCGA CGGGCGCTGT GAGCAGTACC GATGCTACTG CCATTGCTGC CACCACCGAA 
GCCACAACAG TCCCCATCAT CCCAACTGTC GCACCTACCA CCATCGCCAC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCTGCC GCCACCACCA CCACGGAGAG TCCTCCCACC 
ACCACCTCCG GGACTAAGAT ACACGAATCC GGTGCCATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856226.1
CDS	91..3492
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESGAI

Target ORF information:

RefSeq Version	XM_017000737.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000737.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTGCGA CGGGCGCTGT GAGCAGTACC GATGCTACTG CCATTGCTGC CACCACCGAA 
GCCACAACAG TCCCCATCAT CCCAACTGTC GCACCTACCA CCATCGCCAC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCTGCC GCCACCACCA CCACGGAGAG TCCTCCCACC 
ACCACCTCCG GGACTAAGAT ACACGAATCC GGTGCCATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000738.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856227.1 Homo sapiens neurofascin (NFASC), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OHu87056
Clone ID Related Accession (Same CDS sequence)	XM_017000738.1
Accession Version	XM_017000738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCAACCACC CTTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCAG AAAGAACACC AAGCTTCATG 
TATCCCCAGG GCACCGCGAG CAGCCAGATG GTGCTTCGTG GCATGGACCT CCTGCTGGAA 
TGCATCGCCT CCGGGGTCCC AACACCAGAC ATCGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTGATA AGGCCAAGTT TGAGAACTTT AATAAGGCCC TGCGTATCAC AAATGTCTCT 
GAGGAAGACT CCGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ACGAACCCAA GAACCTTATT 
CTGGCTCCTG GCGAGGATGG GAGACTGGTG TGTCGAGCCA ATGGAAACCC CAAACCCACT 
GTCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCTAACCC AAACCGTGAG 
GTGGCCGGAG ACACCATCAT CTTCCGGGAC ACCCAGATCA GCAGCAGGGC TGTGTACCAG 
TGCAACACCT CCAACGAGCA TGGCTACCTG CTGGCCAACG CCTTTGTCAG TGTGCTGGAT 
GTGCCGCCTC GGATGCTGTC GCCCCGGAAC CAGCTCATTC GAGTGATTCT TTACAACCGG 
ACGCGGCTGG ACTGCCCTTT CTTTGGGTCT CCCATCCCCA CACTGCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGTGGCAAC TACCATGTTT ATGAGAACGG CAGTCTGGAA 
ATTAAGATGA TCCGCAAAGA GGACCAGGGC ATCTACACCT GTGTCGCCAC CAACATCCTG 
GGCAAAGCTG AAAACCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACCGGATG 
CCCGAGGACC AGGTGGCCAG AAGGGGCACC ACGGTGCAGC TGGAGTGTCG GGTGAAGCAC 
GACCCCTCCC TGAAACTCAC CGTCTCCTGG CTGAAGGATG ACGAGCCGCT CTATATTGGA 
AACAGGATGA AGAAGGAAGA CGACTCCCTG ACCATCTTTG GGGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTGTCGC CAGCACCGAG CTAGACCAAG ACCTGGCCAA GGCCTACCTC 
ACCGTGCTAG GACGGCCAGA CCGGCCCCGG GACCTGGAGC TGACCGACCT GGCCGAGAGG 
AGCGTGCGGC TGACCTGGAT CCCCGGGGAT GCTAACAACA GCCCCATCAC AGACTACGTC 
GTCCAGTTTG AAGAAGACCA GTTCCAACCT GGGGTCTGGC ATGACCATTC CAAGTACCCC 
GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG TCCCCGTATG TCAACTACCA GTTCCGTGTC 
ATTGCCATCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTCC CATCCGAGCG CTACCGAACC 
AGTGGAGCAC CCCCCGAGTC CAATCCTGGT GACGTGAAGG GAGAGGGGAC CAGAAAGAAC 
AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCGG CCTTTGGCCC CAACCTGCGC 
TACATTGTCA AGTGGAGGCG GAGAGAGACT CGAGAGGCCT GGAACAACGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACCCCA GTCTACGTGC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTCGGGAA GGGCCCTGAG CCAGAGTCCG TCATCGGTTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAGTCCGAG TCATGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGCGT CTACTCCGAC ACGGTCCAGG GCCAGCTCAG AGAGTACCGA 
GCCTACTACT GGAGGGAGAG CAGCTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAAGCCAGCT TCCCTGGTGA CCGCCTCCGT GGCGTGGTGT CCCGCCTCTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT TGTGGTCAAT GGGAGAGGTG ATGGGCCTCG CAGTGAGACC 
AAGGAGTTCA CCACCCCGGA AGGAGTACCC AGTGCCCCTA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCTG AGCATCCAAA TGGGATCATG 
ATTGGATACA CTCTCAAATA TGTGGCCTTT AACGGGACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAACGGACCC CGTGTCACGC 
TACCGCTTTA CCCTCAGCGC CAGGACGCAG GTGGGCTCTG GGGAAGCCGT CACAGAGGAG 
TCACCAGCAC CCCCGAATGA AGCTACTCCA ACCGCAGCTT ACACCAACAA CCAAGCGGAC 
ATCGCCACCC AGGGCTGGTT CATTGGGCTT ATGTGCGCCA TCGCCCTCCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCAGT ACGAGAAAAG 
AAGGATGTTC CCCTTGGCCC TGAAGACCCC AAGGAAGAGG ATGGCTCATT TGACTATAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGT CAGACATCTC TGGACGGCAC CATCAAGCAG 
CAGGAGAGTG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGTCA GTTCAATGAA 
GACGGCTCCT TCATCGGCCA GTACACGGTC AAAAAGGACA AGGAGGAAAC AGAGGGCAAC 
GAAAGCTCAG AGGCCACGTC ACCTGTCAAT GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856227.1
CDS	25..3855
Translation	MRRLAARGAAGDAGEVAAPAADSSDSAQGRSPSPWRLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000738.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000738.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCAACCACC CTTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCAG AAAGAACACC AAGCTTCATG 
TATCCCCAGG GCACCGCGAG CAGCCAGATG GTGCTTCGTG GCATGGACCT CCTGCTGGAA 
TGCATCGCCT CCGGGGTCCC AACACCAGAC ATCGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTGATA AGGCCAAGTT TGAGAACTTT AATAAGGCCC TGCGTATCAC AAATGTCTCT 
GAGGAAGACT CCGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ACGAACCCAA GAACCTTATT 
CTGGCTCCTG GCGAGGATGG GAGACTGGTG TGTCGAGCCA ATGGAAACCC CAAACCCACT 
GTCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCTAACCC AAACCGTGAG 
GTGGCCGGAG ACACCATCAT CTTCCGGGAC ACCCAGATCA GCAGCAGGGC TGTGTACCAG 
TGCAACACCT CCAACGAGCA TGGCTACCTG CTGGCCAACG CCTTTGTCAG TGTGCTGGAT 
GTGCCGCCTC GGATGCTGTC GCCCCGGAAC CAGCTCATTC GAGTGATTCT TTACAACCGG 
ACGCGGCTGG ACTGCCCTTT CTTTGGGTCT CCCATCCCCA CACTGCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGTGGCAAC TACCATGTTT ATGAGAACGG CAGTCTGGAA 
ATTAAGATGA TCCGCAAAGA GGACCAGGGC ATCTACACCT GTGTCGCCAC CAACATCCTG 
GGCAAAGCTG AAAACCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACCGGATG 
CCCGAGGACC AGGTGGCCAG AAGGGGCACC ACGGTGCAGC TGGAGTGTCG GGTGAAGCAC 
GACCCCTCCC TGAAACTCAC CGTCTCCTGG CTGAAGGATG ACGAGCCGCT CTATATTGGA 
AACAGGATGA AGAAGGAAGA CGACTCCCTG ACCATCTTTG GGGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTGTCGC CAGCACCGAG CTAGACCAAG ACCTGGCCAA GGCCTACCTC 
ACCGTGCTAG GACGGCCAGA CCGGCCCCGG GACCTGGAGC TGACCGACCT GGCCGAGAGG 
AGCGTGCGGC TGACCTGGAT CCCCGGGGAT GCTAACAACA GCCCCATCAC AGACTACGTC 
GTCCAGTTTG AAGAAGACCA GTTCCAACCT GGGGTCTGGC ATGACCATTC CAAGTACCCC 
GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG TCCCCGTATG TCAACTACCA GTTCCGTGTC 
ATTGCCATCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTCC CATCCGAGCG CTACCGAACC 
AGTGGAGCAC CCCCCGAGTC CAATCCTGGT GACGTGAAGG GAGAGGGGAC CAGAAAGAAC 
AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCGG CCTTTGGCCC CAACCTGCGC 
TACATTGTCA AGTGGAGGCG GAGAGAGACT CGAGAGGCCT GGAACAACGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACCCCA GTCTACGTGC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTCGGGAA GGGCCCTGAG CCAGAGTCCG TCATCGGTTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAGTCCGAG TCATGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGCGT CTACTCCGAC ACGGTCCAGG GCCAGCTCAG AGAGTACCGA 
GCCTACTACT GGAGGGAGAG CAGCTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAAGCCAGCT TCCCTGGTGA CCGCCTCCGT GGCGTGGTGT CCCGCCTCTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT TGTGGTCAAT GGGAGAGGTG ATGGGCCTCG CAGTGAGACC 
AAGGAGTTCA CCACCCCGGA AGGAGTACCC AGTGCCCCTA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCTG AGCATCCAAA TGGGATCATG 
ATTGGATACA CTCTCAAATA TGTGGCCTTT AACGGGACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAACGGACCC CGTGTCACGC 
TACCGCTTTA CCCTCAGCGC CAGGACGCAG GTGGGCTCTG GGGAAGCCGT CACAGAGGAG 
TCACCAGCAC CCCCGAATGA AGCTACTCCA ACCGCAGCTT ACACCAACAA CCAAGCGGAC 
ATCGCCACCC AGGGCTGGTT CATTGGGCTT ATGTGCGCCA TCGCCCTCCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCAGT ACGAGAAAAG 
AAGGATGTTC CCCTTGGCCC TGAAGACCCC AAGGAAGAGG ATGGCTCATT TGACTATAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGT CAGACATCTC TGGACGGCAC CATCAAGCAG 
CAGGAGAGTG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGTCA GTTCAATGAA 
GACGGCTCCT TCATCGGCCA GTACACGGTC AAAAAGGACA AGGAGGAAAC AGAGGGCAAC 
GAAAGCTCAG AGGCCACGTC ACCTGTCAAT GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000739.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856228.1 Homo sapiens neurofascin (NFASC), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu87057
Clone ID Related Accession (Same CDS sequence)	XM_017000739.1
Accession Version	XM_017000739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCAACCACC CTTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCAG AAAGAACACC AAGCTTCATG 
TATCCCCAGG GCACCGCGAG CAGCCAGATG GTGCTTCGTG GCATGGACCT CCTGCTGGAA 
TGCATCGCCT CCGGGGTCCC AACACCAGAC ATCGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTGATA AGGCCAAGTT TGAGAACTTT AATAAGGCCC TGCGTATCAC AAATGTCTCT 
GAGGAAGACT CCGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ACGAACCCAA GAACCTTATT 
CTGGCTCCTG GCGAGGATGG GAGACTGGTG TGTCGAGCCA ATGGAAACCC CAAACCCACT 
GTCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCTAACCC AAACCGTGAG 
GTGGCCGGAG ACACCATCAT CTTCCGGGAC ACCCAGATCA GCAGCAGGGC TGTGTACCAG 
TGCAACACCT CCAACGAGCA TGGCTACCTG CTGGCCAACG CCTTTGTCAG TGTGCTGGAT 
GTGCCGCCTC GGATGCTGTC GCCCCGGAAC CAGCTCATTC GAGTGATTCT TTACAACCGG 
ACGCGGCTGG ACTGCCCTTT CTTTGGGTCT CCCATCCCCA CACTGCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGTGGCAAC TACCATGTTT ATGAGAACGG CAGTCTGGAA 
ATTAAGATGA TCCGCAAAGA GGACCAGGGC ATCTACACCT GTGTCGCCAC CAACATCCTG 
GGCAAAGCTG AAAACCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACCGGATG 
CCCGAGGACC AGGTGGCCAG AAGGGGCACC ACGGTGCAGC TGGAGTGTCG GGTGAAGCAC 
GACCCCTCCC TGAAACTCAC CGTCTCCTGG CTGAAGGATG ACGAGCCGCT CTATATTGGA 
AACAGGATGA AGAAGGAAGA CGACTCCCTG ACCATCTTTG GGGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTGTCGC CAGCACCGAG CTAGACCAAG ACCTGGCCAA GGCCTACCTC 
ACCGTGCTAG GACGGCCAGA CCGGCCCCGG GACCTGGAGC TGACCGACCT GGCCGAGAGG 
AGCGTGCGGC TGACCTGGAT CCCCGGGGAT GCTAACAACA GCCCCATCAC AGACTACGTC 
GTCCAGTTTG AAGAAGACCA GTTCCAACCT GGGGTCTGGC ATGACCATTC CAAGTACCCC 
GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG TCCCCGTATG TCAACTACCA GTTCCGTGTC 
ATTGCCATCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTCC CATCCGAGCG CTACCGAACC 
AGTGGAGCAC CCCCCGAGTC CAATCCTGGT GACGTGAAGG GAGAGGGGAC CAGAAAGAAC 
AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCGG CCTTTGGCCC CAACCTGCGC 
TACATTGTCA AGTGGAGGCG GAGAGAGACT CGAGAGGCCT GGAACAACGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACCCCA GTCTACGTGC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTCGGGAA GGGCCCTGAG CCAGAGTCCG TCATCGGTTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAGTCCGAG TCATGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGCGT CTACTCCGAC ACGGTCCAGG GCCAGCTCAG AGAGTACCGA 
GCCTACTACT GGAGGGAGAG CAGCTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAAGCCAGCT TCCCTGGTGA CCGCCTCCGT GGCGTGGTGT CCCGCCTCTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT TGTGGTCAAT GGGAGAGGTG ATGGGCCTCG CAGTGAGACC 
AAGGAGTTCA CCACCCCGGA AGGAGTACCC AGTGCCCCTA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCTG AGCATCCAAA TGGGATCATG 
ATTGGATACA CTCTCAAATA TGTGGCCTTT AACGGGACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAACGGACCC CGTGTCACGC 
TACCGCTTTA CCCTCAGCGC CAGGACGCAG GTGGGCTCTG GGGAAGCCGT CACAGAGGAG 
TCACCAGCAC CCCCGAATGA AGCTTACACC AACAACCAAG CGGACATCGC CACCCAGGGC 
TGGTTCATTG GGCTTATGTG CGCCATCGCC CTCCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCAGTACGAG AAAAGAAGGA TGTTCCCCTT 
GGCCCTGAAG ACCCCAAGGA AGAGGATGGC TCATTTGACT ATAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGTCAGAC ATCTCTGGAC GGCACCATCA AGCAGCAGGA GAGTGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGTCAGTTCA ATGAAGACGG CTCCTTCATC 
GGCCAGTACA CGGTCAAAAA GGACAAGGAG GAAACAGAGG GCAACGAAAG CTCAGAGGCC 
ACGTCACCTG TCAATGCTAT CTACTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856228.1
CDS	25..3840
Translation	MRRLAARGAAGDAGEVAAPAADSSDSAQGRSPSPWRLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000739.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000739.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCAACCACC CTTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCAG AAAGAACACC AAGCTTCATG 
TATCCCCAGG GCACCGCGAG CAGCCAGATG GTGCTTCGTG GCATGGACCT CCTGCTGGAA 
TGCATCGCCT CCGGGGTCCC AACACCAGAC ATCGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTGATA AGGCCAAGTT TGAGAACTTT AATAAGGCCC TGCGTATCAC AAATGTCTCT 
GAGGAAGACT CCGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ACGAACCCAA GAACCTTATT 
CTGGCTCCTG GCGAGGATGG GAGACTGGTG TGTCGAGCCA ATGGAAACCC CAAACCCACT 
GTCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCTAACCC AAACCGTGAG 
GTGGCCGGAG ACACCATCAT CTTCCGGGAC ACCCAGATCA GCAGCAGGGC TGTGTACCAG 
TGCAACACCT CCAACGAGCA TGGCTACCTG CTGGCCAACG CCTTTGTCAG TGTGCTGGAT 
GTGCCGCCTC GGATGCTGTC GCCCCGGAAC CAGCTCATTC GAGTGATTCT TTACAACCGG 
ACGCGGCTGG ACTGCCCTTT CTTTGGGTCT CCCATCCCCA CACTGCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGTGGCAAC TACCATGTTT ATGAGAACGG CAGTCTGGAA 
ATTAAGATGA TCCGCAAAGA GGACCAGGGC ATCTACACCT GTGTCGCCAC CAACATCCTG 
GGCAAAGCTG AAAACCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACCGGATG 
CCCGAGGACC AGGTGGCCAG AAGGGGCACC ACGGTGCAGC TGGAGTGTCG GGTGAAGCAC 
GACCCCTCCC TGAAACTCAC CGTCTCCTGG CTGAAGGATG ACGAGCCGCT CTATATTGGA 
AACAGGATGA AGAAGGAAGA CGACTCCCTG ACCATCTTTG GGGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTGTCGC CAGCACCGAG CTAGACCAAG ACCTGGCCAA GGCCTACCTC 
ACCGTGCTAG GACGGCCAGA CCGGCCCCGG GACCTGGAGC TGACCGACCT GGCCGAGAGG 
AGCGTGCGGC TGACCTGGAT CCCCGGGGAT GCTAACAACA GCCCCATCAC AGACTACGTC 
GTCCAGTTTG AAGAAGACCA GTTCCAACCT GGGGTCTGGC ATGACCATTC CAAGTACCCC 
GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG TCCCCGTATG TCAACTACCA GTTCCGTGTC 
ATTGCCATCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTCC CATCCGAGCG CTACCGAACC 
AGTGGAGCAC CCCCCGAGTC CAATCCTGGT GACGTGAAGG GAGAGGGGAC CAGAAAGAAC 
AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCGG CCTTTGGCCC CAACCTGCGC 
TACATTGTCA AGTGGAGGCG GAGAGAGACT CGAGAGGCCT GGAACAACGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACCCCA GTCTACGTGC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTCGGGAA GGGCCCTGAG CCAGAGTCCG TCATCGGTTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAGTCCGAG TCATGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGCGT CTACTCCGAC ACGGTCCAGG GCCAGCTCAG AGAGTACCGA 
GCCTACTACT GGAGGGAGAG CAGCTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAAGCCAGCT TCCCTGGTGA CCGCCTCCGT GGCGTGGTGT CCCGCCTCTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT TGTGGTCAAT GGGAGAGGTG ATGGGCCTCG CAGTGAGACC 
AAGGAGTTCA CCACCCCGGA AGGAGTACCC AGTGCCCCTA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCTG AGCATCCAAA TGGGATCATG 
ATTGGATACA CTCTCAAATA TGTGGCCTTT AACGGGACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAACGGACCC CGTGTCACGC 
TACCGCTTTA CCCTCAGCGC CAGGACGCAG GTGGGCTCTG GGGAAGCCGT CACAGAGGAG 
TCACCAGCAC CCCCGAATGA AGCTTACACC AACAACCAAG CGGACATCGC CACCCAGGGC 
TGGTTCATTG GGCTTATGTG CGCCATCGCC CTCCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCAGTACGAG AAAAGAAGGA TGTTCCCCTT 
GGCCCTGAAG ACCCCAAGGA AGAGGATGGC TCATTTGACT ATAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGTCAGAC ATCTCTGGAC GGCACCATCA AGCAGCAGGA GAGTGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGTCAGTTCA ATGAAGACGG CTCCTTCATC 
GGCCAGTACA CGGTCAAAAA GGACAAGGAG GAAACAGAGG GCAACGAAAG CTCAGAGGCC 
ACGTCACCTG TCAATGCTAT CTACTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_017000740.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856229.1 Homo sapiens neurofascin (NFASC), transcript variant X30, mRNA.

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CloneID	OHu87058
Clone ID Related Accession (Same CDS sequence)	XM_017000740.1
Accession Version	XM_017000740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCAACCACC CTTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCAG AAAGAACACC AAGCTTCATG 
TATCCCCAGG GCACCGCGAG CAGCCAGATG GTGCTTCGTG GCATGGACCT CCTGCTGGAA 
TGCATCGCCT CCGGGGTCCC AACACCAGAC ATCGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTGATA AGGCCAAGTT TGAGAACTTT AATAAGGCCC TGCGTATCAC AAATGTCTCT 
GAGGAAGACT CCGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ACGAACCCAA GAACCTTATT 
CTGGCTCCTG GCGAGGATGG GAGACTGGTG TGTCGAGCCA ATGGAAACCC CAAACCCACT 
GTCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCTAACCC AAACCGTGAG 
GTGGCCGGAG ACACCATCAT CTTCCGGGAC ACCCAGATCA GCAGCAGGGC TGTGTACCAG 
TGCAACACCT CCAACGAGCA TGGCTACCTG CTGGCCAACG CCTTTGTCAG TGTGCTGGAT 
GTGCCGCCTC GGATGCTGTC GCCCCGGAAC CAGCTCATTC GAGTGATTCT TTACAACCGG 
ACGCGGCTGG ACTGCCCTTT CTTTGGGTCT CCCATCCCCA CACTGCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGTGGCAAC TACCATGTTT ATGAGAACGG CAGTCTGGAA 
ATTAAGATGA TCCGCAAAGA GGACCAGGGC ATCTACACCT GTGTCGCCAC CAACATCCTG 
GGCAAAGCTG AAAACCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACCGGATG 
CCCGAGGACC AGGTGGCCAG AAGGGGCACC ACGGTGCAGC TGGAGTGTCG GGTGAAGCAC 
GACCCCTCCC TGAAACTCAC CGTCTCCTGG CTGAAGGATG ACGAGCCGCT CTATATTGGA 
AACAGGATGA AGAAGGAAGA CGACTCCCTG ACCATCTTTG GGGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTGTCGC CAGCACCGAG CTAGACCAAG ACCTGGCCAA GGCCTACCTC 
ACCGTGCTAG GACGGCCAGA CCGGCCCCGG GACCTGGAGC TGACCGACCT GGCCGAGAGG 
AGCGTGCGGC TGACCTGGAT CCCCGGGGAT GCTAACAACA GCCCCATCAC AGACTACGTC 
GTCCAGTTTG AAGAAGACCA GTTCCAACCT GGGGTCTGGC ATGACCATTC CAAGTACCCC 
GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG TCCCCGTATG TCAACTACCA GTTCCGTGTC 
ATTGCCATCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTCC CATCCGAGCG CTACCGAACC 
AGTGGAGCAC CCCCCGAGTC CAATCCTGGT GACGTGAAGG GAGAGGGGAC CAGAAAGAAC 
AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCGG CCTTTGGCCC CAACCTGCGC 
TACATTGTCA AGTGGAGGCG GAGAGAGACT CGAGAGGCCT GGAACAACGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACCCCA GTCTACGTGC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTCGGGAA GGGCCCTGAG CCAGAGTCCG TCATCGGTTA CTCCGGAGAA 
GATTTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTTACACCA ACAACCAAGC GGACATCGCC ACCCAGGGCT GGTTCATTGG GCTTATGTGC 
GCCATCGCCC TCCTGGTGCT GATCCTGCTC ATCGTCTGTT TCATCAAGAG GAGTCGCGGC 
GGCAAGTACC CAGTACGAGA AAAGAAGGAT GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA 
GAGGATGGCT CATTTGACTA TAGTGATGAG GACAACAAGC CCCTGCAGGG CAGTCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTGGTGGA CTATGGCGAG 
GGTGGCGAGG GTCAGTTCAA TGAAGACGGC TCCTTCATCG GCCAGTACAC GGTCAAAAAG 
GACAAGGAGG AAACAGAGGG CAACGAAAGC TCAGAGGCCA CGTCACCTGT CAATGCTATC 
TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856229.1
CDS	25..3519
Translation	MRRLAARGAAGDAGEVAAPAADSSDSAQGRSPSPWRLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017000740.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000740.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCA 
GCGGACAGCT CGGACAGCGC CCAGGGCCGG AGCCCGAGCC CTTGGAGGTT GATTGACTTA 
TGTGCAATTT GGGACGCTGG AGTTTACCTT CCCTCCGCAG CCTGGAACAG AGCCTCCTCT 
GGTGTTGCAA GGAAGAGGCT GAATGAGGCA GAGAAGCTGA GTGCTGTCCA GGAGGCCCAG 
TTAAAGCGGC TCGAGGTGAC AAGACCCCGA GTGCTGGGGA GCAGGGAGCA GGGCCAGGTG 
CCGAGGATGG CCAGGCAGCC ACCGCCGCCC TGGGTCCATG CAGCCTTCCT CCTCTGCCTC 
CTCAGTCTTG GCGGAGCCAT CGAAATTCCT ATGGATCTGA CGCAGCCGCC AACCATCACC 
AAGCAGTCAG CGAAGGATCA CATCGTGGAC CCCCGTGATA ACATCCTGAT TGAGTGTGAA 
GCAAAAGGGA ACCCTGCCCC CAGCTTCCAC TGGACACGAA ACAGCAGATT CTTCAACATC 
GCCAAGGACC CCCGGGTGTC CATGAGGAGG AGGTCTGGGA CCCTGGTGAT TGACTTCCGC 
AGTGGCGGGC GGCCGGAGGA ATATGAGGGG GAATATCAGT GCTTCGCCCG CAACAAATTT 
GGCACGGCCC TGTCCAATAG GATCCGCCTG CAGGTGTCTA AATCTCCTCT GTGGCCCAAG 
GAAAACCTAG ACCCTGTCGT GGTCCAAGAG GGCGCTCCTT TGACGCTCCA GTGCAACCCC 
CCGCCTGGAC TTCCATCCCC GGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAAGACAAAC GTGTCTCTCA GGGCCATAAC GGAGACCTAT ACTTCTCCAA CGTGATGCTG 
CAGGACATGC AGACCGACTA CAGTTGTAAC GCCCGCTTCC ACTTCACCCA CACCATCCAG 
CAGAAGAACC CTTTCACCCT CAAGGTCCTC ACCAACCACC CTTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCAG AAAGAACACC AAGCTTCATG 
TATCCCCAGG GCACCGCGAG CAGCCAGATG GTGCTTCGTG GCATGGACCT CCTGCTGGAA 
TGCATCGCCT CCGGGGTCCC AACACCAGAC ATCGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTGATA AGGCCAAGTT TGAGAACTTT AATAAGGCCC TGCGTATCAC AAATGTCTCT 
GAGGAAGACT CCGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ACGAACCCAA GAACCTTATT 
CTGGCTCCTG GCGAGGATGG GAGACTGGTG TGTCGAGCCA ATGGAAACCC CAAACCCACT 
GTCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCTAACCC AAACCGTGAG 
GTGGCCGGAG ACACCATCAT CTTCCGGGAC ACCCAGATCA GCAGCAGGGC TGTGTACCAG 
TGCAACACCT CCAACGAGCA TGGCTACCTG CTGGCCAACG CCTTTGTCAG TGTGCTGGAT 
GTGCCGCCTC GGATGCTGTC GCCCCGGAAC CAGCTCATTC GAGTGATTCT TTACAACCGG 
ACGCGGCTGG ACTGCCCTTT CTTTGGGTCT CCCATCCCCA CACTGCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGTGGCAAC TACCATGTTT ATGAGAACGG CAGTCTGGAA 
ATTAAGATGA TCCGCAAAGA GGACCAGGGC ATCTACACCT GTGTCGCCAC CAACATCCTG 
GGCAAAGCTG AAAACCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACCGGATG 
CCCGAGGACC AGGTGGCCAG AAGGGGCACC ACGGTGCAGC TGGAGTGTCG GGTGAAGCAC 
GACCCCTCCC TGAAACTCAC CGTCTCCTGG CTGAAGGATG ACGAGCCGCT CTATATTGGA 
AACAGGATGA AGAAGGAAGA CGACTCCCTG ACCATCTTTG GGGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTGTCGC CAGCACCGAG CTAGACCAAG ACCTGGCCAA GGCCTACCTC 
ACCGTGCTAG GACGGCCAGA CCGGCCCCGG GACCTGGAGC TGACCGACCT GGCCGAGAGG 
AGCGTGCGGC TGACCTGGAT CCCCGGGGAT GCTAACAACA GCCCCATCAC AGACTACGTC 
GTCCAGTTTG AAGAAGACCA GTTCCAACCT GGGGTCTGGC ATGACCATTC CAAGTACCCC 
GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG TCCCCGTATG TCAACTACCA GTTCCGTGTC 
ATTGCCATCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTCC CATCCGAGCG CTACCGAACC 
AGTGGAGCAC CCCCCGAGTC CAATCCTGGT GACGTGAAGG GAGAGGGGAC CAGAAAGAAC 
AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCGG CCTTTGGCCC CAACCTGCGC 
TACATTGTCA AGTGGAGGCG GAGAGAGACT CGAGAGGCCT GGAACAACGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACCCCA GTCTACGTGC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTCGGGAA GGGCCCTGAG CCAGAGTCCG TCATCGGTTA CTCCGGAGAA 
GATTTACCCA GTGCCCCTAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCTGA GCATCCAAAT GGGATCATGA TTGGATACAC TCTCAAATAT 
GTGGCCTTTA ACGGGACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGGTGCAAAG AACGGACCCC GTGTCACGCT ACCGCTTTAC CCTCAGCGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCCGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTTACACCA ACAACCAAGC GGACATCGCC ACCCAGGGCT GGTTCATTGG GCTTATGTGC 
GCCATCGCCC TCCTGGTGCT GATCCTGCTC ATCGTCTGTT TCATCAAGAG GAGTCGCGGC 
GGCAAGTACC CAGTACGAGA AAAGAAGGAT GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA 
GAGGATGGCT CATTTGACTA TAGTGATGAG GACAACAAGC CCCTGCAGGG CAGTCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTGGTGGA CTATGGCGAG 
GGTGGCGAGG GTCAGTTCAA TGAAGACGGC TCCTTCATCG GCCAGTACAC GGTCAAAAAG 
GACAAGGAGG AAACAGAGGG CAACGAAAGC TCAGAGGCCA CGTCACCTGT CAATGCTATC 
TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001160332.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153804.1 Homo sapiens neurofascin (NFASC), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01378
Clone ID Related Accession (Same CDS sequence)	NM_001160332.1
Accession Version	NM_001160332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	neurofascin isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC096675.2, AL391822.12 and BC035389.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences in the N-terminal and central regions, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCAAC CACCCTTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTAC TCCAACCGCA GCTTACACCA ACAACCAAGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTTATGTGC GCCATCGCCC TCCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CAGTACGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA GAGGATGGCT CATTTGACTA TAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGTCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GTCAGTTCAA TGAAGACGGC 
TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAGGAGG AAACAGAGGG CAACGAAAGC 
TCAGAGGCCA CGTCACCTGT CAATGCTATC TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153804.1
CDS	329..3853
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160332.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160332.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCAAC CACCCTTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTAC TCCAACCGCA GCTTACACCA ACAACCAAGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTTATGTGC GCCATCGCCC TCCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CAGTACGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA GAGGATGGCT CATTTGACTA TAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGTCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GTCAGTTCAA TGAAGACGGC 
TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAGGAGG AAACAGAGGG CAACGAAAGC 
TCAGAGGCCA CGTCACCTGT CAATGCTATC TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_015090.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055905.2 Homo sapiens neurofascin (NFASC), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu02001
Clone ID Related Accession (Same CDS sequence)	NM_015090.3
Accession Version	NM_015090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	neurofascin isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC040674.1, AB018299.3, AL391822.12 and BC035389.1. On May 21, 2009 this sequence version replaced NM_015090.2. Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (4) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences in the N-terminal and central regions, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB018299.3, BC137013.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCAAC CACCCTTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055905.2
CDS	329..3838
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_015090.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015090.3

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CTGACGCAGC CGCCAACCAT CACCAAGCAG 
TCAGCGAAGG ATCACATCGT GGACCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACA CGAAACAGCA GATTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCCATGAG GAGGAGGTCT GGGACCCTGG TGATTGACTT CCGCAGTGGC 
GGGCGGCCGG AGGAATATGA GGGGGAATAT CAGTGCTTCG CCCGCAACAA ATTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCTAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTGGTCCA AGAGGGCGCT CCTTTGACGC TCCAGTGCAA CCCCCCGCCT 
GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAACGTGTCT CTCAGGGCCA TAACGGAGAC CTATACTTCT CCAACGTGAT GCTGCAGGAC 
ATGCAGACCG ACTACAGTTG TAACGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCTTTCA CCCTCAAGGT CCTCACCAAC CACCCTTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTTA CACCAACAAC CAAGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTTA TGTGCGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCAGTA CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAAGACCCCA AGGAAGAGGA TGGCTCATTT GACTATAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGTC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGTCAG TTCAATGAAG ACGGCTCCTT CATCGGCCAG 
TACACGGTCA AAAAGGACAA GGAGGAAACA GAGGGCAACG AAAGCTCAGA GGCCACGTCA 
CCTGTCAATG CTATCTACTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509321.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507623.1 Homo sapiens neurofascin (NFASC), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu65468
Clone ID Related Accession (Same CDS sequence)	XM_011509321.1
Accession Version	XM_011509321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCCCCTG ATGAGCAGTC CATATGGAAC 
GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAAGCACAA TTTCGGGCCC 
GGAACTGACT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCAGTT 
AAGGCCCAGG CTCAGCCTAT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG 
CGGGTTTATT CCCGGGACAA CGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT 
ACAGCTTACA CCAACAACCA AGCGGACATC GCCACCCAGG GCTGGTTCAT TGGGCTTATG 
TGCGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC 
GGCGGCAAGT ACCCAGTACG AGAAAAGAAG GATGTTCCCC TTGGCCCTGA AGACCCCAAG 
GAAGAGGATG GCTCATTTGA CTATAGTGAT GAGGACAACA AGCCCCTGCA GGGCAGTCAG 
ACATCTCTGG ACGGCACCAT CAAGCAGCAG GAGAGTGACG ACAGCCTGGT GGACTATGGC 
GAGGGTGGCG AGGGTCAGTT CAATGAAGAC GGCTCCTTCA TCGGCCAGTA CACGGTCAAA 
AAGGACAAGG AGGAAACAGA GGGCAACGAA AGCTCAGAGG CCACGTCACC TGTCAATGCT 
ATCTACTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507623.1
CDS	91..3948
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509321.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509321.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCCCCTG ATGAGCAGTC CATATGGAAC 
GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAAGCACAA TTTCGGGCCC 
GGAACTGACT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCAGTT 
AAGGCCCAGG CTCAGCCTAT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG 
CGGGTTTATT CCCGGGACAA CGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT 
ACAGCTTACA CCAACAACCA AGCGGACATC GCCACCCAGG GCTGGTTCAT TGGGCTTATG 
TGCGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC 
GGCGGCAAGT ACCCAGTACG AGAAAAGAAG GATGTTCCCC TTGGCCCTGA AGACCCCAAG 
GAAGAGGATG GCTCATTTGA CTATAGTGAT GAGGACAACA AGCCCCTGCA GGGCAGTCAG 
ACATCTCTGG ACGGCACCAT CAAGCAGCAG GAGAGTGACG ACAGCCTGGT GGACTATGGC 
GAGGGTGGCG AGGGTCAGTT CAATGAAGAC GGCTCCTTCA TCGGCCAGTA CACGGTCAAA 
AAGGACAAGG AGGAAACAGA GGGCAACGAA AGCTCAGAGG CCACGTCACC TGTCAATGCT 
ATCTACTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509324.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507626.1 Homo sapiens neurofascin (NFASC), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu65471
Clone ID Related Accession (Same CDS sequence)	XM_011509324.1
Accession Version	XM_011509324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTTACA CCAACAACCA AGCGGACATC 
GCCACCCAGG GCTGGTTCAT TGGGCTTATG TGCGCCATCG CCCTCCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCAGTACG AGAAAAGAAG 
GATGTTCCCC TTGGCCCTGA AGACCCCAAG GAAGAGGATG GCTCATTTGA CTATAGTGAT 
GAGGACAACA AGCCCCTGCA GGGCAGTCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG 
GAGAGTGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGTCAGTT CAATGAAGAC 
GGCTCCTTCA TCGGCCAGTA CACGGTCAAA AAGGACAAGG AGGAAACAGA GGGCAACGAA 
AGCTCAGAGG CCACGTCACC TGTCAATGCT ATCTACTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507626.1
CDS	91..3678
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509324.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509324.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTTACA CCAACAACCA AGCGGACATC 
GCCACCCAGG GCTGGTTCAT TGGGCTTATG TGCGCCATCG CCCTCCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCAGTACG AGAAAAGAAG 
GATGTTCCCC TTGGCCCTGA AGACCCCAAG GAAGAGGATG GCTCATTTGA CTATAGTGAT 
GAGGACAACA AGCCCCTGCA GGGCAGTCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG 
GAGAGTGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGTCAGTT CAATGAAGAC 
GGCTCCTTCA TCGGCCAGTA CACGGTCAAA AAGGACAAGG AGGAAACAGA GGGCAACGAA 
AGCTCAGAGG CCACGTCACC TGTCAATGCT ATCTACTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509325.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507627.1 Homo sapiens neurofascin (NFASC), transcript variant X18, mRNA.

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CloneID	OHu65472
Clone ID Related Accession (Same CDS sequence)	XM_011509325.1
Accession Version	XM_011509325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TTACACCAAC AACCAAGCGG ACATCGCCAC CCAGGGCTGG 
TTCATTGGGC TTATGTGCGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCA GTACGAGAAA AGAAGGATGT TCCCCTTGGC 
CCTGAAGACC CCAAGGAAGA GGATGGCTCA TTTGACTATA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GTCAGACATC TCTGGACGGC ACCATCAAGC AGCAGGAGAG TGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGT CAGTTCAATG AAGACGGCTC CTTCATCGGC 
CAGTACACGG TCAAAAAGGA CAAGGAGGAA ACAGAGGGCA ACGAAAGCTC AGAGGCCACG 
TCACCTGTCA ATGCTATCTA CTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507627.1
CDS	91..3663
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509325.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509325.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA GTCCGAGTCA TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC AGCTCAGAGA GTACCGAGCC 
TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA 
GCCAGCTTCC CTGGTGACCG CCTCCGTGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGCAG TGAGACCAAG 
GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCTAGGC GTTTCCGAGT CCGGCAGCCC 
AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCTGAGC ATCCAAATGG GATCATGATT 
GGATACACTC TCAAATATGT GGCCTTTAAC GGGACCAAAG TAGGAAAGCA GATAGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAA CGGACCCCGT GTCACGCTAC 
CGCTTTACCC TCAGCGCCAG GACGCAGGTG GGCTCTGGGG AAGCCGTCAC AGAGGAGTCA 
CCAGCACCCC CGAATGAAGC TTACACCAAC AACCAAGCGG ACATCGCCAC CCAGGGCTGG 
TTCATTGGGC TTATGTGCGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCA GTACGAGAAA AGAAGGATGT TCCCCTTGGC 
CCTGAAGACC CCAAGGAAGA GGATGGCTCA TTTGACTATA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GTCAGACATC TCTGGACGGC ACCATCAAGC AGCAGGAGAG TGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGT CAGTTCAATG AAGACGGCTC CTTCATCGGC 
CAGTACACGG TCAAAAAGGA CAAGGAGGAA ACAGAGGGCA ACGAAAGCTC AGAGGCCACG 
TCACCTGTCA ATGCTATCTA CTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005244989.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245046.2 Homo sapiens neurofascin (NFASC), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu65473
Clone ID Related Accession (Same CDS sequence)	XM_005244989.3
Accession Version	XM_005244989.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005244989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGCTGAT 
CAGGCCACTC CAACTAACCG TTTGGCTGCC CTGCCCAAAG GACGGCCAGA CCGGCCCCGG 
GACCTGGAGC TGACCGACCT GGCCGAGAGG AGCGTGCGGC TGACCTGGAT CCCCGGGGAT 
GCTAACAACA GCCCCATCAC AGACTACGTC GTCCAGTTTG AAGAAGACCA GTTCCAACCT 
GGGGTCTGGC ATGACCATTC CAAGTACCCC GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG 
TCCCCGTATG TCAACTACCA GTTCCGTGTC ATTGCCATCA ACGAGGTTGG GAGCAGCCAC 
CCCAGCCTCC CATCCGAGCG CTACCGAACC AGTGGAGCAC CCCCCGAGTC CAATCCTGGT 
GACGTGAAGG GAGAGGGGAC CAGAAAGAAC AACATGGAGA TCACGTGGAC GCCCATGAAT 
GCCACCTCGG CCTTTGGCCC CAACCTGCGC TACATTGTCA AGTGGAGGCG GAGAGAGACT 
CGAGAGGCCT GGAACAACGT CACAGTGTGG GGCTCTCGCT ACGTGGTGGG GCAGACCCCA 
GTCTACGTGC CCTATGAGAT CCGAGTCCAG GCTGAAAATG ACTTCGGGAA GGGCCCTGAG 
CCAGAGTCCG TCATCGGTTA CTCCGGAGAA GATTATCCCA GGGCTGCGCC CACTGAAGTT 
AAAGTCCGAG TCATGAACAG CACAGCCATC AGCCTTCAGT GGAACCGCGT CTACTCCGAC 
ACGGTCCAGG GCCAGCTCAG AGAGTACCGA GCCTACTACT GGAGGGAGAG CAGCTTGCTG 
AAGAACCTGT GGGTGTCTCA GAAGAGACAG CAAGCCAGCT TCCCTGGTGA CCGCCTCCGT 
GGCGTGGTGT CCCGCCTCTT CCCCTACAGT AACTACAAGC TGGAGATGGT TGTGGTCAAT 
GGGAGAGGTG ATGGGCCTCG CAGTGAGACC AAGGAGTTCA CCACCCCGGA AGGAGTACCC 
AGTGCCCCTA GGCGTTTCCG AGTCCGGCAG CCCAACCTGG AGACAATCAA CCTGGAATGG 
GATCATCCTG AGCATCCAAA TGGGATCATG ATTGGATACA CTCTCAAATA TGTGGCCTTT 
AACGGGACCA AAGTAGGAAA GCAGATAGTG GAAAACTTCT CTCCCAATCA GACCAAGTTC 
ACGGTGCAAA GAACGGACCC CGTGTCACGC TACCGCTTTA CCCTCAGCGC CAGGACGCAG 
GTGGGCTCTG GGGAAGCCGT CACAGAGGAG TCACCAGCAC CCCCGAATGA AGCTACTCCA 
ACCGCAGCTT ACACCAACAA CCAAGCGGAC ATCGCCACCC AGGGCTGGTT CATTGGGCTT 
ATGTGCGCCA TCGCCCTCCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCAGT ACGAGAAAAG AAGGATGTTC CCCTTGGCCC TGAAGACCCC 
AAGGAAGAGG ATGGCTCATT TGACTATAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGT 
CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGTCA GTTCAATGAA GACGGCTCCT TCATCGGCCA GTACACGGTC 
AAAAAGGACA AGGAGGAAAC AGAGGGCAAC GAAAGCTCAG AGGCCACGTC ACCTGTCAAT 
GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245046.2
CDS	178..3978
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005244989.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005244989.3

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGCTGAT 
CAGGCCACTC CAACTAACCG TTTGGCTGCC CTGCCCAAAG GACGGCCAGA CCGGCCCCGG 
GACCTGGAGC TGACCGACCT GGCCGAGAGG AGCGTGCGGC TGACCTGGAT CCCCGGGGAT 
GCTAACAACA GCCCCATCAC AGACTACGTC GTCCAGTTTG AAGAAGACCA GTTCCAACCT 
GGGGTCTGGC ATGACCATTC CAAGTACCCC GGCAGCGTTA ACTCAGCCGT CCTCCGGCTG 
TCCCCGTATG TCAACTACCA GTTCCGTGTC ATTGCCATCA ACGAGGTTGG GAGCAGCCAC 
CCCAGCCTCC CATCCGAGCG CTACCGAACC AGTGGAGCAC CCCCCGAGTC CAATCCTGGT 
GACGTGAAGG GAGAGGGGAC CAGAAAGAAC AACATGGAGA TCACGTGGAC GCCCATGAAT 
GCCACCTCGG CCTTTGGCCC CAACCTGCGC TACATTGTCA AGTGGAGGCG GAGAGAGACT 
CGAGAGGCCT GGAACAACGT CACAGTGTGG GGCTCTCGCT ACGTGGTGGG GCAGACCCCA 
GTCTACGTGC CCTATGAGAT CCGAGTCCAG GCTGAAAATG ACTTCGGGAA GGGCCCTGAG 
CCAGAGTCCG TCATCGGTTA CTCCGGAGAA GATTATCCCA GGGCTGCGCC CACTGAAGTT 
AAAGTCCGAG TCATGAACAG CACAGCCATC AGCCTTCAGT GGAACCGCGT CTACTCCGAC 
ACGGTCCAGG GCCAGCTCAG AGAGTACCGA GCCTACTACT GGAGGGAGAG CAGCTTGCTG 
AAGAACCTGT GGGTGTCTCA GAAGAGACAG CAAGCCAGCT TCCCTGGTGA CCGCCTCCGT 
GGCGTGGTGT CCCGCCTCTT CCCCTACAGT AACTACAAGC TGGAGATGGT TGTGGTCAAT 
GGGAGAGGTG ATGGGCCTCG CAGTGAGACC AAGGAGTTCA CCACCCCGGA AGGAGTACCC 
AGTGCCCCTA GGCGTTTCCG AGTCCGGCAG CCCAACCTGG AGACAATCAA CCTGGAATGG 
GATCATCCTG AGCATCCAAA TGGGATCATG ATTGGATACA CTCTCAAATA TGTGGCCTTT 
AACGGGACCA AAGTAGGAAA GCAGATAGTG GAAAACTTCT CTCCCAATCA GACCAAGTTC 
ACGGTGCAAA GAACGGACCC CGTGTCACGC TACCGCTTTA CCCTCAGCGC CAGGACGCAG 
GTGGGCTCTG GGGAAGCCGT CACAGAGGAG TCACCAGCAC CCCCGAATGA AGCTACTCCA 
ACCGCAGCTT ACACCAACAA CCAAGCGGAC ATCGCCACCC AGGGCTGGTT CATTGGGCTT 
ATGTGCGCCA TCGCCCTCCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCAGT ACGAGAAAAG AAGGATGTTC CCCTTGGCCC TGAAGACCCC 
AAGGAAGAGG ATGGCTCATT TGACTATAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGT 
CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGTCA GTTCAATGAA GACGGCTCCT TCATCGGCCA GTACACGGTC 
AAAAAGGACA AGGAGGAAAC AGAGGGCAAC GAAAGCTCAG AGGCCACGTC ACCTGTCAAT 
GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005244991.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245048.2 Homo sapiens neurofascin (NFASC), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu65474
Clone ID Related Accession (Same CDS sequence)	XM_005244991.3
Accession Version	XM_005244991.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005244991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGGACGG 
CCAGACCGGC CCCGGGACCT GGAGCTGACC GACCTGGCCG AGAGGAGCGT GCGGCTGACC 
TGGATCCCCG GGGATGCTAA CAACAGCCCC ATCACAGACT ACGTCGTCCA GTTTGAAGAA 
GACCAGTTCC AACCTGGGGT CTGGCATGAC CATTCCAAGT ACCCCGGCAG CGTTAACTCA 
GCCGTCCTCC GGCTGTCCCC GTATGTCAAC TACCAGTTCC GTGTCATTGC CATCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTCCCATCC GAGCGCTACC GAACCAGTGG AGCACCCCCC 
GAGTCCAATC CTGGTGACGT GAAGGGAGAG GGGACCAGAA AGAACAACAT GGAGATCACG 
TGGACGCCCA TGAATGCCAC CTCGGCCTTT GGCCCCAACC TGCGCTACAT TGTCAAGTGG 
AGGCGGAGAG AGACTCGAGA GGCCTGGAAC AACGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CCCCAGTCTA CGTGCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTC 
GGGAAGGGCC CTGAGCCAGA GTCCGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAGT CCGAGTCATG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGCGTCTACT CCGACACGGT CCAGGGCCAG CTCAGAGAGT ACCGAGCCTA CTACTGGAGG 
GAGAGCAGCT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAAGC CAGCTTCCCT 
GGTGACCGCC TCCGTGGCGT GGTGTCCCGC CTCTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTTGTGG TCAATGGGAG AGGTGATGGG CCTCGCAGTG AGACCAAGGA GTTCACCACC 
CCGGAAGGAG TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTTACACC AACAACCAAG CGGACATCGC CACCCAGGGC 
TGGTTCATTG GGCTTATGTG CGCCATCGCC CTCCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCAGTACGAG AAAAGAAGGA TGTTCCCCTT 
GGCCCTGAAG ACCCCAAGGA AGAGGATGGC TCATTTGACT ATAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGTCAGAC ATCTCTGGAC GGCACCATCA AGCAGCAGGA GAGTGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGTCAGTTCA ATGAAGACGG CTCCTTCATC 
GGCCAGTACA CGGTCAAAAA GGACAAGGAG GAAACAGAGG GCAACGAAAG CTCAGAGGCC 
ACGTCACCTG TCAATGCTAT CTACTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245048.2
CDS	178..3933
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005244991.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005244991.3

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGGACGG 
CCAGACCGGC CCCGGGACCT GGAGCTGACC GACCTGGCCG AGAGGAGCGT GCGGCTGACC 
TGGATCCCCG GGGATGCTAA CAACAGCCCC ATCACAGACT ACGTCGTCCA GTTTGAAGAA 
GACCAGTTCC AACCTGGGGT CTGGCATGAC CATTCCAAGT ACCCCGGCAG CGTTAACTCA 
GCCGTCCTCC GGCTGTCCCC GTATGTCAAC TACCAGTTCC GTGTCATTGC CATCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTCCCATCC GAGCGCTACC GAACCAGTGG AGCACCCCCC 
GAGTCCAATC CTGGTGACGT GAAGGGAGAG GGGACCAGAA AGAACAACAT GGAGATCACG 
TGGACGCCCA TGAATGCCAC CTCGGCCTTT GGCCCCAACC TGCGCTACAT TGTCAAGTGG 
AGGCGGAGAG AGACTCGAGA GGCCTGGAAC AACGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CCCCAGTCTA CGTGCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTC 
GGGAAGGGCC CTGAGCCAGA GTCCGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAGT CCGAGTCATG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGCGTCTACT CCGACACGGT CCAGGGCCAG CTCAGAGAGT ACCGAGCCTA CTACTGGAGG 
GAGAGCAGCT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAAGC CAGCTTCCCT 
GGTGACCGCC TCCGTGGCGT GGTGTCCCGC CTCTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTTGTGG TCAATGGGAG AGGTGATGGG CCTCGCAGTG AGACCAAGGA GTTCACCACC 
CCGGAAGGAG TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTTACACC AACAACCAAG CGGACATCGC CACCCAGGGC 
TGGTTCATTG GGCTTATGTG CGCCATCGCC CTCCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCAGTACGAG AAAAGAAGGA TGTTCCCCTT 
GGCCCTGAAG ACCCCAAGGA AGAGGATGGC TCATTTGACT ATAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGTCAGAC ATCTCTGGAC GGCACCATCA AGCAGCAGGA GAGTGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGTCAGTTCA ATGAAGACGG CTCCTTCATC 
GGCCAGTACA CGGTCAAAAA GGACAAGGAG GAAACAGAGG GCAACGAAAG CTCAGAGGCC 
ACGTCACCTG TCAATGCTAT CTACTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005244992.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245049.2 Homo sapiens neurofascin (NFASC), transcript variant X23, mRNA.

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CloneID	OHu65475
Clone ID Related Accession (Same CDS sequence)	XM_005244992.4
Accession Version	XM_005244992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005244992.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGGACGG 
CCAGACCGGC CCCGGGACCT GGAGCTGACC GACCTGGCCG AGAGGAGCGT GCGGCTGACC 
TGGATCCCCG GGGATGCTAA CAACAGCCCC ATCACAGACT ACGTCGTCCA GTTTGAAGAA 
GACCAGTTCC AACCTGGGGT CTGGCATGAC CATTCCAAGT ACCCCGGCAG CGTTAACTCA 
GCCGTCCTCC GGCTGTCCCC GTATGTCAAC TACCAGTTCC GTGTCATTGC CATCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTCCCATCC GAGCGCTACC GAACCAGTGG AGCACCCCCC 
GAGTCCAATC CTGGTGACGT GAAGGGAGAG GGGACCAGAA AGAACAACAT GGAGATCACG 
TGGACGCCCA TGAATGCCAC CTCGGCCTTT GGCCCCAACC TGCGCTACAT TGTCAAGTGG 
AGGCGGAGAG AGACTCGAGA GGCCTGGAAC AACGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CCCCAGTCTA CGTGCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTC 
GGGAAGGGCC CTGAGCCAGA GTCCGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAGT CCGAGTCATG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGCGTCTACT CCGACACGGT CCAGGGCCAG CTCAGAGAGT ACCGAGCCTA CTACTGGAGG 
GAGAGCAGCT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAAGC CAGCTTCCCT 
GGTGACCGCC TCCGTGGCGT GGTGTCCCGC CTCTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTTGTGG TCAATGGGAG AGGTGATGGG CCTCGCAGTG AGACCAAGGA GTTCACCACC 
CCGGAAGGAG TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTT ACACCAACAA CCAAGCGGAC ATCGCCACCC AGGGCTGGTT CATTGGGCTT 
ATGTGCGCCA TCGCCCTCCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCAGT ACGAGAAAAG AAGGATGTTC CCCTTGGCCC TGAAGACCCC 
AAGGAAGAGG ATGGCTCATT TGACTATAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGT 
CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGTCA GTTCAATGAA GACGGCTCCT TCATCGGCCA GTACACGGTC 
AAAAAGGACA AGGAGGAAAC AGAGGGCAAC GAAAGCTCAG AGGCCACGTC ACCTGTCAAT 
GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245049.2
CDS	178..3918
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005244992.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005244992.4

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAA CCACCCTTAT AATGACTCGT CCTTAAGAAA CCACCCTGAC 
ATGTACAGTG CCCGAGGAGT TGCAGAAAGA ACACCAAGCT TCATGTATCC CCAGGGCACC 
GCGAGCAGCC AGATGGTGCT TCGTGGCATG GACCTCCTGC TGGAATGCAT CGCCTCCGGG 
GTCCCAACAC CAGACATCGC ATGGTACAAG AAAGGTGGGG ACCTCCCATC TGATAAGGCC 
AAGTTTGAGA ACTTTAATAA GGCCCTGCGT ATCACAAATG TCTCTGAGGA AGACTCCGGG 
GAGTATTTCT GCCTGGCCTC CAACAAGATG GGCAGCATCC GGCACACGAT CTCGGTGAGA 
GTAAAGGCTG CTCCCTACTG GCTGGACGAA CCCAAGAACC TTATTCTGGC TCCTGGCGAG 
GATGGGAGAC TGGTGTGTCG AGCCAATGGA AACCCCAAAC CCACTGTCCA GTGGATGGTG 
AATGGGGAAC CTTTGCAATC GGCACCACCT AACCCAAACC GTGAGGTGGC CGGAGACACC 
ATCATCTTCC GGGACACCCA GATCAGCAGC AGGGCTGTGT ACCAGTGCAA CACCTCCAAC 
GAGCATGGCT ACCTGCTGGC CAACGCCTTT GTCAGTGTGC TGGATGTGCC GCCTCGGATG 
CTGTCGCCCC GGAACCAGCT CATTCGAGTG ATTCTTTACA ACCGGACGCG GCTGGACTGC 
CCTTTCTTTG GGTCTCCCAT CCCCACACTG CGATGGTTTA AGAATGGGCA AGGAAGCAAC 
CTGGATGGTG GCAACTACCA TGTTTATGAG AACGGCAGTC TGGAAATTAA GATGATCCGC 
AAAGAGGACC AGGGCATCTA CACCTGTGTC GCCACCAACA TCCTGGGCAA AGCTGAAAAC 
CAAGTCCGCC TGGAGGTCAA AGACCCCACC AGGATCTACC GGATGCCCGA GGACCAGGTG 
GCCAGAAGGG GCACCACGGT GCAGCTGGAG TGTCGGGTGA AGCACGACCC CTCCCTGAAA 
CTCACCGTCT CCTGGCTGAA GGATGACGAG CCGCTCTATA TTGGAAACAG GATGAAGAAG 
GAAGACGACT CCCTGACCAT CTTTGGGGTG GCAGAGCGGG ACCAGGGCAG TTACACGTGT 
GTCGCCAGCA CCGAGCTAGA CCAAGACCTG GCCAAGGCCT ACCTCACCGT GCTAGGACGG 
CCAGACCGGC CCCGGGACCT GGAGCTGACC GACCTGGCCG AGAGGAGCGT GCGGCTGACC 
TGGATCCCCG GGGATGCTAA CAACAGCCCC ATCACAGACT ACGTCGTCCA GTTTGAAGAA 
GACCAGTTCC AACCTGGGGT CTGGCATGAC CATTCCAAGT ACCCCGGCAG CGTTAACTCA 
GCCGTCCTCC GGCTGTCCCC GTATGTCAAC TACCAGTTCC GTGTCATTGC CATCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTCCCATCC GAGCGCTACC GAACCAGTGG AGCACCCCCC 
GAGTCCAATC CTGGTGACGT GAAGGGAGAG GGGACCAGAA AGAACAACAT GGAGATCACG 
TGGACGCCCA TGAATGCCAC CTCGGCCTTT GGCCCCAACC TGCGCTACAT TGTCAAGTGG 
AGGCGGAGAG AGACTCGAGA GGCCTGGAAC AACGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CCCCAGTCTA CGTGCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTC 
GGGAAGGGCC CTGAGCCAGA GTCCGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAGT CCGAGTCATG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGCGTCTACT CCGACACGGT CCAGGGCCAG CTCAGAGAGT ACCGAGCCTA CTACTGGAGG 
GAGAGCAGCT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAAGC CAGCTTCCCT 
GGTGACCGCC TCCGTGGCGT GGTGTCCCGC CTCTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTTGTGG TCAATGGGAG AGGTGATGGG CCTCGCAGTG AGACCAAGGA GTTCACCACC 
CCGGAAGGAG TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTT ACACCAACAA CCAAGCGGAC ATCGCCACCC AGGGCTGGTT CATTGGGCTT 
ATGTGCGCCA TCGCCCTCCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCAGT ACGAGAAAAG AAGGATGTTC CCCTTGGCCC TGAAGACCCC 
AAGGAAGAGG ATGGCTCATT TGACTATAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGT 
CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGTCA GTTCAATGAA GACGGCTCCT TCATCGGCCA GTACACGGTC 
AAAAAGGACA AGGAGGAAAC AGAGGGCAAC GAAAGCTCAG AGGCCACGTC ACCTGTCAAT 
GCTATCTACT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005244993.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245050.2 Homo sapiens neurofascin (NFASC), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu65476
Clone ID Related Accession (Same CDS sequence)	XM_005244993.3
Accession Version	XM_005244993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005244993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTAC TCCAACCGCA GCTTACACCA ACAACCAAGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTTATGTGC GCCATCGCCC TCCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CAGTACGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA GAGGATGGCT CATTTGACTA TAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGTCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GTCAGTTCAA TGAAGACGGC 
TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAGGAGG AAACAGAGGG CAACGAAAGC 
TCAGAGGCCA CGTCACCTGT CAATGCTATC TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245050.2
CDS	178..3882
Translation	MNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005244993.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005244993.3

ORF Insert Sequence:

ATGAACCAGA CCATGGCTCC TTCAGGAGAG AGGTTGATTG ACTTATGTGC AATTTGGGAC 
GCTGGAGTTT ACCTTCCCTC CGCAGCCTGG AACAGAGCCT CCTCTGGTGT TGCAAGGAAG 
AGGCTGAATG AGGCAGAGAA GCTGAGTGCT GTCCAGGAGG CCCAGTTAAA GCGGCTCGAG 
GTGACAAGAC CCCGAGTGCT GGGGAGCAGG GAGCAGGGCC AGGTGCCGAG GATGGCCAGG 
CAGCCACCGC CGCCCTGGGT CCATGCAGCC TTCCTCCTCT GCCTCCTCAG TCTTGGCGGA 
GCCATCGAAA TTCCTATGGA TCTGACGCAG CCGCCAACCA TCACCAAGCA GTCAGCGAAG 
GATCACATCG TGGACCCCCG TGATAACATC CTGATTGAGT GTGAAGCAAA AGGGAACCCT 
GCCCCCAGCT TCCACTGGAC ACGAAACAGC AGATTCTTCA ACATCGCCAA GGACCCCCGG 
GTGTCCATGA GGAGGAGGTC TGGGACCCTG GTGATTGACT TCCGCAGTGG CGGGCGGCCG 
GAGGAATATG AGGGGGAATA TCAGTGCTTC GCCCGCAACA AATTTGGCAC GGCCCTGTCC 
AATAGGATCC GCCTGCAGGT GTCTAAATCT CCTCTGTGGC CCAAGGAAAA CCTAGACCCT 
GTCGTGGTCC AAGAGGGCGC TCCTTTGACG CTCCAGTGCA ACCCCCCGCC TGGACTTCCA 
TCCCCGGTCA TCTTCTGGAT GAGCAGCTCC ATGGAGCCCA TCACCCAAGA CAAACGTGTC 
TCTCAGGGCC ATAACGGAGA CCTATACTTC TCCAACGTGA TGCTGCAGGA CATGCAGACC 
GACTACAGTT GTAACGCCCG CTTCCACTTC ACCCACACCA TCCAGCAGAA GAACCCTTTC 
ACCCTCAAGG TCCTCACCAC CCGAGGAGTT GCAGAAAGAA CACCAAGCTT CATGTATCCC 
CAGGGCACCG CGAGCAGCCA GATGGTGCTT CGTGGCATGG ACCTCCTGCT GGAATGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GATAAGGCCA AGTTTGAGAA CTTTAATAAG GCCCTGCGTA TCACAAATGT CTCTGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATTCTGGCT 
CCTGGCGAGG ATGGGAGACT GGTGTGTCGA GCCAATGGAA ACCCCAAACC CACTGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCTA ACCCAAACCG TGAGGTGGCC 
GGAGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCTGTGTA CCAGTGCAAC 
ACCTCCAACG AGCATGGCTA CCTGCTGGCC AACGCCTTTG TCAGTGTGCT GGATGTGCCG 
CCTCGGATGC TGTCGCCCCG GAACCAGCTC ATTCGAGTGA TTCTTTACAA CCGGACGCGG 
CTGGACTGCC CTTTCTTTGG GTCTCCCATC CCCACACTGC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTTTATGAGA ACGGCAGTCT GGAAATTAAG 
ATGATCCGCA AAGAGGACCA GGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA 
GCTGAAAACC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCCGAG 
GACCAGGTGG CCAGAAGGGG CACCACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC 
TCCCTGAAAC TCACCGTCTC CTGGCTGAAG GATGACGAGC CGCTCTATAT TGGAAACAGG 
ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGGGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTG TCGCCAGCAC CGAGCTAGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG 
CTAGGACGGC CAGACCGGCC CCGGGACCTG GAGCTGACCG ACCTGGCCGA GAGGAGCGTG 
CGGCTGACCT GGATCCCCGG GGATGCTAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC TGGCATGACC ATTCCAAGTA CCCCGGCAGC 
GTTAACTCAG CCGTCCTCCG GCTGTCCCCG TATGTCAACT ACCAGTTCCG TGTCATTGCC 
ATCAACGAGG TTGGGAGCAG CCACCCCAGC CTCCCATCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCCG AGTCCAATCC TGGTGACGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAATGCCACC TCGGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGA GGCGGAGAGA GACTCGAGAG GCCTGGAACA ACGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC CCCAGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTCG GGAAGGGCCC TGAGCCAGAG TCCGTCATCG GTTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAGTC CGAGTCATGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC 
AGCTTCCCTG GTGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCTGAGCATC CAAATGGGAT CATGATTGGA 
TACACTCTCA AATATGTGGC CTTTAACGGG ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAACGG ACCCCGTGTC ACGCTACCGC 
TTTACCCTCA GCGCCAGGAC GCAGGTGGGC TCTGGGGAAG CCGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTAC TCCAACCGCA GCTTACACCA ACAACCAAGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTTATGTGC GCCATCGCCC TCCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CAGTACGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAAGA CCCCAAGGAA GAGGATGGCT CATTTGACTA TAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGTCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GTCAGTTCAA TGAAGACGGC 
TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAGGAGG AAACAGAGGG CAACGAAAGC 
TCAGAGGCCA CGTCACCTGT CAATGCTATC TACTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509326.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507628.1 Homo sapiens neurofascin (NFASC), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OHu65477
Clone ID Related Accession (Same CDS sequence)	XM_011509326.1
Accession Version	XM_011509326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TTACCCAGTG CCCCTAGGCG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG 
GAATGGGATC ATCCTGAGCA TCCAAATGGG ATCATGATTG GATACACTCT CAAATATGTG 
GCCTTTAACG GGACCAAAGT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCACGG TGCAAAGAAC GGACCCCGTG TCACGCTACC GCTTTACCCT CAGCGCCAGG 
ACGCAGGTGG GCTCTGGGGA AGCCGTCACA GAGGAGTCAC CAGCACCCCC GAATGAAGCT 
ACTCCAACCG CAGCTTACAC CAACAACCAA GCGGACATCG CCACCCAGGG CTGGTTCATT 
GGGCTTATGT GCGCCATCGC CCTCCTGGTG CTGATCCTGC TCATCGTCTG TTTCATCAAG 
AGGAGTCGCG GCGGCAAGTA CCCAGTACGA GAAAAGAAGG ATGTTCCCCT TGGCCCTGAA 
GACCCCAAGG AAGAGGATGG CTCATTTGAC TATAGTGATG AGGACAACAA GCCCCTGCAG 
GGCAGTCAGA CATCTCTGGA CGGCACCATC AAGCAGCAGG AGAGTGACGA CAGCCTGGTG 
GACTATGGCG AGGGTGGCGA GGGTCAGTTC AATGAAGACG GCTCCTTCAT CGGCCAGTAC 
ACGGTCAAAA AGGACAAGGA GGAAACAGAG GGCAACGAAA GCTCAGAGGC CACGTCACCT 
GTCAATGCTA TCTACTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507628.1
CDS	91..3357
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509326.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509326.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TTACCCAGTG CCCCTAGGCG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG 
GAATGGGATC ATCCTGAGCA TCCAAATGGG ATCATGATTG GATACACTCT CAAATATGTG 
GCCTTTAACG GGACCAAAGT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCACGG TGCAAAGAAC GGACCCCGTG TCACGCTACC GCTTTACCCT CAGCGCCAGG 
ACGCAGGTGG GCTCTGGGGA AGCCGTCACA GAGGAGTCAC CAGCACCCCC GAATGAAGCT 
ACTCCAACCG CAGCTTACAC CAACAACCAA GCGGACATCG CCACCCAGGG CTGGTTCATT 
GGGCTTATGT GCGCCATCGC CCTCCTGGTG CTGATCCTGC TCATCGTCTG TTTCATCAAG 
AGGAGTCGCG GCGGCAAGTA CCCAGTACGA GAAAAGAAGG ATGTTCCCCT TGGCCCTGAA 
GACCCCAAGG AAGAGGATGG CTCATTTGAC TATAGTGATG AGGACAACAA GCCCCTGCAG 
GGCAGTCAGA CATCTCTGGA CGGCACCATC AAGCAGCAGG AGAGTGACGA CAGCCTGGTG 
GACTATGGCG AGGGTGGCGA GGGTCAGTTC AATGAAGACG GCTCCTTCAT CGGCCAGTAC 
ACGGTCAAAA AGGACAAGGA GGAAACAGAG GGCAACGAAA GCTCAGAGGC CACGTCACCT 
GTCAATGCTA TCTACTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509327.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507629.1 Homo sapiens neurofascin (NFASC), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OHu65478
Clone ID Related Accession (Same CDS sequence)	XM_011509327.1
Accession Version	XM_011509327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TTACCCAGTG CCCCTAGGCG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG 
GAATGGGATC ATCCTGAGCA TCCAAATGGG ATCATGATTG GATACACTCT CAAATATGTG 
GCCTTTAACG GGACCAAAGT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCACGG TGCAAAGAAC GGACCCCGTG TCACGCTACC GCTTTACCCT CAGCGCCAGG 
ACGCAGGTGG GCTCTGGGGA AGCCGTCACA GAGGAGTCAC CAGCACCCCC GAATGAAGCT 
TACACCAACA ACCAAGCGGA CATCGCCACC CAGGGCTGGT TCATTGGGCT TATGTGCGCC 
ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCAG TACGAGAAAA GAAGGATGTT CCCCTTGGCC CTGAAGACCC CAAGGAAGAG 
GATGGCTCAT TTGACTATAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG TCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGTC AGTTCAATGA AGACGGCTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC 
AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA GAGGCCACGT CACCTGTCAA TGCTATCTAC 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507629.1
CDS	91..3342
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509327.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509327.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA 
CCAAGCTTCA TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC 
CTCCTGCTGG AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC 
ACAAATGTCT CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC 
AAGAACCTTA TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC 
CCCAAACCCA CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC 
CCAAACCGTG AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG 
GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC 
AGTGTGCTGG ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT 
CTTTACAACC GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC 
GGCAGTCTGG AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC 
ACCAACATCC TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACCGGA TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT 
CGGGTGAAGC ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG 
CTCTATATTG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC 
AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGAC GGCCAGACCG GCCCCGGGAC CTGGAGCTGA CCGACCTGGC CGAGAGGAGC 
GTGCGGCTGA CCTGGATCCC CGGGGATGCT AACAACAGCC CCATCACAGA CTACGTCGTC 
CAGTTTGAAG AAGACCAGTT CCAACCTGGG GTCTGGCATG ACCATTCCAA GTACCCCGGC 
AGCGTTAACT CAGCCGTCCT CCGGCTGTCC CCGTATGTCA ACTACCAGTT CCGTGTCATT 
GCCATCAACG AGGTTGGGAG CAGCCACCCC AGCCTCCCAT CCGAGCGCTA CCGAACCAGT 
GGAGCACCCC CCGAGTCCAA TCCTGGTGAC GTGAAGGGAG AGGGGACCAG AAAGAACAAC 
ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCGGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGAGGCGGAG AGAGACTCGA GAGGCCTGGA ACAACGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACCCCAGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TCGGGAAGGG CCCTGAGCCA GAGTCCGTCA TCGGTTACTC CGGAGAAGAT 
TTACCCAGTG CCCCTAGGCG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG 
GAATGGGATC ATCCTGAGCA TCCAAATGGG ATCATGATTG GATACACTCT CAAATATGTG 
GCCTTTAACG GGACCAAAGT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCACGG TGCAAAGAAC GGACCCCGTG TCACGCTACC GCTTTACCCT CAGCGCCAGG 
ACGCAGGTGG GCTCTGGGGA AGCCGTCACA GAGGAGTCAC CAGCACCCCC GAATGAAGCT 
TACACCAACA ACCAAGCGGA CATCGCCACC CAGGGCTGGT TCATTGGGCT TATGTGCGCC 
ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCAG TACGAGAAAA GAAGGATGTT CCCCTTGGCC CTGAAGACCC CAAGGAAGAG 
GATGGCTCAT TTGACTATAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG TCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGTC AGTTCAATGA AGACGGCTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC 
AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA GAGGCCACGT CACCTGTCAA TGCTATCTAC 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509328.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507630.1 Homo sapiens neurofascin (NFASC), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OHu65480
Clone ID Related Accession (Same CDS sequence)	XM_011509328.1
Accession Version	XM_011509328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTTACACC AACAACCAAG CGGACATCGC CACCCAGGGC 
TGGTTCATTG GGCTTATGTG CGCCATCGCC CTCCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCAGTACGAG AAAAGAAGGA TGTTCCCCTT 
GGCCCTGAAG ACCCCAAGGA AGAGGATGGC TCATTTGACT ATAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGTCAGAC ATCTCTGGAC GGCACCATCA AGCAGCAGGA GAGTGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGTCAGTTCA ATGAAGACGG CTCCTTCATC 
GGCCAGTACA CGGTCAAAAA GGACAAGGAG GAAACAGAGG GCAACGAAAG CTCAGAGGCC 
ACGTCACCTG TCAATGCTAT CTACTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507630.1
CDS	91..3306
Translation	MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509328.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509328.1

ORF Insert Sequence:

ATGGCCAGGC AGCCACCGCC GCCCTGGGTC CATGCAGCCT TCCTCCTCTG CCTCCTCAGT 
CTTGGCGGAG CCATCGAAAT TCCTATGGAT CCAAGCATTC AGAATGAGCT GACGCAGCCG 
CCAACCATCA CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG 
ATTGAGTGTG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG 
ATTGACTTCC GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC 
CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC 
CAGTGCAACC CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC 
AACGTGATGC TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACAC CAAGCTTCAT GTATCCCCAG GGCACCGCGA GCAGCCAGAT GGTGCTTCGT 
GGCATGGACC TCCTGCTGGA ATGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAT AAGGCCAAGT TTGAGAACTT TAATAAGGCC 
CTGCGTATCA CAAATGTCTC TGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT TCTGGCTCCT GGCGAGGATG GGAGACTGGT GTGTCGAGCC 
AATGGAAACC CCAAACCCAC TGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCTAACC CAAACCGTGA GGTGGCCGGA GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT GCTGGCCAAC 
GCCTTTGTCA GTGTGCTGGA TGTGCCGCCT CGGATGCTGT CGCCCCGGAA CCAGCTCATT 
CGAGTGATTC TTTACAACCG GACGCGGCTG GACTGCCCTT TCTTTGGGTC TCCCATCCCC 
ACACTGCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTT 
TATGAGAACG GCAGTCTGGA AATTAAGATG ATCCGCAAAG AGGACCAGGG CATCTACACC 
TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAAAACCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGGAT GCCCGAGGAC CAGGTGGCCA GAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAACTCA CCGTCTCCTG GCTGAAGGAT 
GACGAGCCGC TCTATATTGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT 
GGGGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTGTCG CCAGCACCGA GCTAGACCAA 
GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGG CCCCGGGACC TGGAGCTGAC CGACCTGGCC 
GAGAGGAGCG TGCGGCTGAC CTGGATCCCC GGGGATGCTA ACAACAGCCC CATCACAGAC 
TACGTCGTCC AGTTTGAAGA AGACCAGTTC CAACCTGGGG TCTGGCATGA CCATTCCAAG 
TACCCCGGCA GCGTTAACTC AGCCGTCCTC CGGCTGTCCC CGTATGTCAA CTACCAGTTC 
CGTGTCATTG CCATCAACGA GGTTGGGAGC AGCCACCCCA GCCTCCCATC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC CGAGTCCAAT CCTGGTGACG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCC ATGAATGCCA CCTCGGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GAGGCGGAGA GAGACTCGAG AGGCCTGGAA CAACGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACCCCAGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT CGGGAAGGGC CCTGAGCCAG AGTCCGTCAT CGGTTACTCC 
GGAGAAGATT TACCCAGTGC CCCTAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCTGAGCAT CCAAATGGGA TCATGATTGG ATACACTCTC 
AAATATGTGG CCTTTAACGG GACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGGT GCAAAGAACG GACCCCGTGT CACGCTACCG CTTTACCCTC 
AGCGCCAGGA CGCAGGTGGG CTCTGGGGAA GCCGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTA CTCCAACCGC AGCTTACACC AACAACCAAG CGGACATCGC CACCCAGGGC 
TGGTTCATTG GGCTTATGTG CGCCATCGCC CTCCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCAGTACGAG AAAAGAAGGA TGTTCCCCTT 
GGCCCTGAAG ACCCCAAGGA AGAGGATGGC TCATTTGACT ATAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGTCAGAC ATCTCTGGAC GGCACCATCA AGCAGCAGGA GAGTGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGTCAGTTCA ATGAAGACGG CTCCTTCATC 
GGCCAGTACA CGGTCAAAAA GGACAAGGAG GAAACAGAGG GCAACGAAAG CTCAGAGGCC 
ACGTCACCTG TCAATGCTAT CTACTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024454283.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310051.1 Homo sapiens neurofascin (NFASC), transcript variant X1, mRNA.

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CloneID	OHu104312
Clone ID Related Accession (Same CDS sequence)	XM_024454283.1
Accession Version	XM_024454283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTAC TCCAACCGCA 
GCTCCTCCCA CATTGCCCCC GACTACCGTG GGTGCGACGG GCGCTGTGAG CAGTACCGAT 
GCTACTGCCA TTGCTGCCAC CACCGAAGCC ACAACAGTCC CCATCATCCC AACTGTCGCA 
CCTACCACCA TCGCCACCAC CACCACCGTC GCCACAACTA CTACAACCAC TGCTGCCGCC 
ACCACCACCA CGGAGAGTCC TCCCACCACC ACCTCCGGGA CTAAGATACA CGAATCCGCC 
CCTGATGAGC AGTCCATATG GAACGTCACG GTGCTCCCCA ACAGTAAATG GGCCAACATC 
ACCTGGAAGC ACAATTTCGG GCCCGGAACT GACTTTGTGG TTGAGTACAT CGACAGCAAC 
CATACGAAAA AAACTGTCCC AGTTAAGGCC CAGGCTCAGC CTATACAGCT GACAGACCTC 
TATCCCGGGA TGACATACAC GTTGCGGGTT TATTCCCGGG ACAACGAGGG CATCAGCAGT 
ACCGTCATCA CCTTTATGAC CAGTACAGCT TACACCAACA ACCAAGCGGA CATCGCCACC 
CAGGGCTGGT TCATTGGGCT TATGTGCGCC ATCGCCCTCC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCAG TACGAGAAAA GAAGGATGTT 
CCCCTTGGCC CTGAAGACCC CAAGGAAGAG GATGGCTCAT TTGACTATAG TGATGAGGAC 
AACAAGCCCC TGCAGGGCAG TCAGACATCT CTGGACGGCA CCATCAAGCA GCAGGAGAGT 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGTC AGTTCAATGA AGACGGCTCC 
TTCATCGGCC AGTACACGGT CAAAAAGGAC AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA 
GAGGCCACGT CACCTGTCAA TGCTATCTAC TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310051.1
CDS	74..4615
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024454283.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454283.1

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTAC TCCAACCGCA 
GCTCCTCCCA CATTGCCCCC GACTACCGTG GGTGCGACGG GCGCTGTGAG CAGTACCGAT 
GCTACTGCCA TTGCTGCCAC CACCGAAGCC ACAACAGTCC CCATCATCCC AACTGTCGCA 
CCTACCACCA TCGCCACCAC CACCACCGTC GCCACAACTA CTACAACCAC TGCTGCCGCC 
ACCACCACCA CGGAGAGTCC TCCCACCACC ACCTCCGGGA CTAAGATACA CGAATCCGCC 
CCTGATGAGC AGTCCATATG GAACGTCACG GTGCTCCCCA ACAGTAAATG GGCCAACATC 
ACCTGGAAGC ACAATTTCGG GCCCGGAACT GACTTTGTGG TTGAGTACAT CGACAGCAAC 
CATACGAAAA AAACTGTCCC AGTTAAGGCC CAGGCTCAGC CTATACAGCT GACAGACCTC 
TATCCCGGGA TGACATACAC GTTGCGGGTT TATTCCCGGG ACAACGAGGG CATCAGCAGT 
ACCGTCATCA CCTTTATGAC CAGTACAGCT TACACCAACA ACCAAGCGGA CATCGCCACC 
CAGGGCTGGT TCATTGGGCT TATGTGCGCC ATCGCCCTCC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCAG TACGAGAAAA GAAGGATGTT 
CCCCTTGGCC CTGAAGACCC CAAGGAAGAG GATGGCTCAT TTGACTATAG TGATGAGGAC 
AACAAGCCCC TGCAGGGCAG TCAGACATCT CTGGACGGCA CCATCAAGCA GCAGGAGAGT 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGTC AGTTCAATGA AGACGGCTCC 
TTCATCGGCC AGTACACGGT CAAAAAGGAC AAGGAGGAAA CAGAGGGCAA CGAAAGCTCA 
GAGGCCACGT CACCTGTCAA TGCTATCTAC TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_011509311.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507613.2 Homo sapiens neurofascin (NFASC), transcript variant X2, mRNA.

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CloneID	OHu104313
Clone ID Related Accession (Same CDS sequence)	XM_011509311.2
Accession Version	XM_011509311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509311.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTCC TCCCACATTG 
CCCCCGACTA CCGTGGGTGC GACGGGCGCT GTGAGCAGTA CCGATGCTAC TGCCATTGCT 
GCCACCACCG AAGCCACAAC AGTCCCCATC ATCCCAACTG TCGCACCTAC CACCATCGCC 
ACCACCACCA CCGTCGCCAC AACTACTACA ACCACTGCTG CCGCCACCAC CACCACGGAG 
AGTCCTCCCA CCACCACCTC CGGGACTAAG ATACACGAAT CCGCCCCTGA TGAGCAGTCC 
ATATGGAACG TCACGGTGCT CCCCAACAGT AAATGGGCCA ACATCACCTG GAAGCACAAT 
TTCGGGCCCG GAACTGACTT TGTGGTTGAG TACATCGACA GCAACCATAC GAAAAAAACT 
GTCCCAGTTA AGGCCCAGGC TCAGCCTATA CAGCTGACAG ACCTCTATCC CGGGATGACA 
TACACGTTGC GGGTTTATTC CCGGGACAAC GAGGGCATCA GCAGTACCGT CATCACCTTT 
ATGACCAGTA CAGCTTACAC CAACAACCAA GCGGACATCG CCACCCAGGG CTGGTTCATT 
GGGCTTATGT GCGCCATCGC CCTCCTGGTG CTGATCCTGC TCATCGTCTG TTTCATCAAG 
AGGAGTCGCG GCGGCAAGTA CCCAGTACGA GAAAAGAAGG ATGTTCCCCT TGGCCCTGAA 
GACCCCAAGG AAGAGGATGG CTCATTTGAC TATAGTGATG AGGACAACAA GCCCCTGCAG 
GGCAGTCAGA CATCTCTGGA CGGCACCATC AAGCAGCAGG AGAGTGACGA CAGCCTGGTG 
GACTATGGCG AGGGTGGCGA GGGTCAGTTC AATGAAGACG GCTCCTTCAT CGGCCAGTAC 
ACGGTCAAAA AGGACAAGGA GGAAACAGAG GGCAACGAAA GCTCAGAGGC CACGTCACCT 
GTCAATGCTA TCTACTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507613.2
CDS	74..4600
Translation	MGVTRSREARNYFDSTQGRAAVFSLRPTFREKKDLAGILLRPKSASWDGLGPKKCLASLPTCPHGGRLRRRWMNQTMAPSGERLIDLCAIWDAGVYLPSAAWNRASSGVARKRLNEAEKLSAVQEAQLKRLEVTRPRVLGSREQGQVPRMARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIATTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011509311.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofascin (NFASC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509311.2

ORF Insert Sequence:

ATGGGGGTGA CAAGGAGCAG GGAAGCCAGG AATTATTTTG ATTCCACCCA GGGCAGGGCT 
GCTGTGTTTT CTCTGAGGCC AACTTTCAGG GAAAAGAAGG ATCTTGCTGG GATTTTGCTC 
AGGCCTAAGT CTGCCAGCTG GGATGGCCTG GGGCCTAAGA AATGCCTAGC TTCCCTCCCC 
ACCTGTCCCC ATGGAGGGAG ACTGAGAAGA CGCTGGATGA ACCAGACCAT GGCTCCTTCA 
GGAGAGAGGT TGATTGACTT ATGTGCAATT TGGGACGCTG GAGTTTACCT TCCCTCCGCA 
GCCTGGAACA GAGCCTCCTC TGGTGTTGCA AGGAAGAGGC TGAATGAGGC AGAGAAGCTG 
AGTGCTGTCC AGGAGGCCCA GTTAAAGCGG CTCGAGGTGA CAAGACCCCG AGTGCTGGGG 
AGCAGGGAGC AGGGCCAGGT GCCGAGGATG GCCAGGCAGC CACCGCCGCC CTGGGTCCAT 
GCAGCCTTCC TCCTCTGCCT CCTCAGTCTT GGCGGAGCCA TCGAAATTCC TATGGATCCA 
AGCATTCAGA ATGAGCTGAC GCAGCCGCCA ACCATCACCA AGCAGTCAGC GAAGGATCAC 
ATCGTGGACC CCCGTGATAA CATCCTGATT GAGTGTGAAG CAAAAGGGAA CCCTGCCCCC 
AGCTTCCACT GGACACGAAA CAGCAGATTC TTCAACATCG CCAAGGACCC CCGGGTGTCC 
ATGAGGAGGA GGTCTGGGAC CCTGGTGATT GACTTCCGCA GTGGCGGGCG GCCGGAGGAA 
TATGAGGGGG AATATCAGTG CTTCGCCCGC AACAAATTTG GCACGGCCCT GTCCAATAGG 
ATCCGCCTGC AGGTGTCTAA ATCTCCTCTG TGGCCCAAGG AAAACCTAGA CCCTGTCGTG 
GTCCAAGAGG GCGCTCCTTT GACGCTCCAG TGCAACCCCC CGCCTGGACT TCCATCCCCG 
GTCATCTTCT GGATGAGCAG CTCCATGGAG CCCATCACCC AAGACAAACG TGTCTCTCAG 
GGCCATAACG GAGACCTATA CTTCTCCAAC GTGATGCTGC AGGACATGCA GACCGACTAC 
AGTTGTAACG CCCGCTTCCA CTTCACCCAC ACCATCCAGC AGAAGAACCC TTTCACCCTC 
AAGGTCCTCA CCAACCACCC TTATAATGAC TCGTCCTTAA GAAACCACCC TGACATGTAC 
AGTGCCCGAG GAGTTGCAGA AAGAACACCA AGCTTCATGT ATCCCCAGGG CACCGCGAGC 
AGCCAGATGG TGCTTCGTGG CATGGACCTC CTGCTGGAAT GCATCGCCTC CGGGGTCCCA 
ACACCAGACA TCGCATGGTA CAAGAAAGGT GGGGACCTCC CATCTGATAA GGCCAAGTTT 
GAGAACTTTA ATAAGGCCCT GCGTATCACA AATGTCTCTG AGGAAGACTC CGGGGAGTAT 
TTCTGCCTGG CCTCCAACAA GATGGGCAGC ATCCGGCACA CGATCTCGGT GAGAGTAAAG 
GCTGCTCCCT ACTGGCTGGA CGAACCCAAG AACCTTATTC TGGCTCCTGG CGAGGATGGG 
AGACTGGTGT GTCGAGCCAA TGGAAACCCC AAACCCACTG TCCAGTGGAT GGTGAATGGG 
GAACCTTTGC AATCGGCACC ACCTAACCCA AACCGTGAGG TGGCCGGAGA CACCATCATC 
TTCCGGGACA CCCAGATCAG CAGCAGGGCT GTGTACCAGT GCAACACCTC CAACGAGCAT 
GGCTACCTGC TGGCCAACGC CTTTGTCAGT GTGCTGGATG TGCCGCCTCG GATGCTGTCG 
CCCCGGAACC AGCTCATTCG AGTGATTCTT TACAACCGGA CGCGGCTGGA CTGCCCTTTC 
TTTGGGTCTC CCATCCCCAC ACTGCGATGG TTTAAGAATG GGCAAGGAAG CAACCTGGAT 
GGTGGCAACT ACCATGTTTA TGAGAACGGC AGTCTGGAAA TTAAGATGAT CCGCAAAGAG 
GACCAGGGCA TCTACACCTG TGTCGCCACC AACATCCTGG GCAAAGCTGA AAACCAAGTC 
CGCCTGGAGG TCAAAGACCC CACCAGGATC TACCGGATGC CCGAGGACCA GGTGGCCAGA 
AGGGGCACCA CGGTGCAGCT GGAGTGTCGG GTGAAGCACG ACCCCTCCCT GAAACTCACC 
GTCTCCTGGC TGAAGGATGA CGAGCCGCTC TATATTGGAA ACAGGATGAA GAAGGAAGAC 
GACTCCCTGA CCATCTTTGG GGTGGCAGAG CGGGACCAGG GCAGTTACAC GTGTGTCGCC 
AGCACCGAGC TAGACCAAGA CCTGGCCAAG GCCTACCTCA CCGTGCTAGC TGATCAGGCC 
ACTCCAACTA ACCGTTTGGC TGCCCTGCCC AAAGGACGGC CAGACCGGCC CCGGGACCTG 
GAGCTGACCG ACCTGGCCGA GAGGAGCGTG CGGCTGACCT GGATCCCCGG GGATGCTAAC 
AACAGCCCCA TCACAGACTA CGTCGTCCAG TTTGAAGAAG ACCAGTTCCA ACCTGGGGTC 
TGGCATGACC ATTCCAAGTA CCCCGGCAGC GTTAACTCAG CCGTCCTCCG GCTGTCCCCG 
TATGTCAACT ACCAGTTCCG TGTCATTGCC ATCAACGAGG TTGGGAGCAG CCACCCCAGC 
CTCCCATCCG AGCGCTACCG AACCAGTGGA GCACCCCCCG AGTCCAATCC TGGTGACGTG 
AAGGGAGAGG GGACCAGAAA GAACAACATG GAGATCACGT GGACGCCCAT GAATGCCACC 
TCGGCCTTTG GCCCCAACCT GCGCTACATT GTCAAGTGGA GGCGGAGAGA GACTCGAGAG 
GCCTGGAACA ACGTCACAGT GTGGGGCTCT CGCTACGTGG TGGGGCAGAC CCCAGTCTAC 
GTGCCCTATG AGATCCGAGT CCAGGCTGAA AATGACTTCG GGAAGGGCCC TGAGCCAGAG 
TCCGTCATCG GTTACTCCGG AGAAGATTAT CCCAGGGCTG CGCCCACTGA AGTTAAAGTC 
CGAGTCATGA ACAGCACAGC CATCAGCCTT CAGTGGAACC GCGTCTACTC CGACACGGTC 
CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC TACTGGAGGG AGAGCAGCTT GCTGAAGAAC 
CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC AGCTTCCCTG GTGACCGCCT CCGTGGCGTG 
GTGTCCCGCC TCTTCCCCTA CAGTAACTAC AAGCTGGAGA TGGTTGTGGT CAATGGGAGA 
GGTGATGGGC CTCGCAGTGA GACCAAGGAG TTCACCACCC CGGAAGGAGT ACCCAGTGCC 
CCTAGGCGTT TCCGAGTCCG GCAGCCCAAC CTGGAGACAA TCAACCTGGA ATGGGATCAT 
CCTGAGCATC CAAATGGGAT CATGATTGGA TACACTCTCA AATATGTGGC CTTTAACGGG 
ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC TTCTCTCCCA ATCAGACCAA GTTCACGGTG 
CAAAGAACGG ACCCCGTGTC ACGCTACCGC TTTACCCTCA GCGCCAGGAC GCAGGTGGGC 
TCTGGGGAAG CCGTCACAGA GGAGTCACCA GCACCCCCGA ATGAAGCTCC TCCCACATTG 
CCCCCGACTA CCGTGGGTGC GACGGGCGCT GTGAGCAGTA CCGATGCTAC TGCCATTGCT 
GCCACCACCG AAGCCACAAC AGTCCCCATC ATCCCAACTG TCGCACCTAC CACCATCGCC 
ACCACCACCA CCGTCGCCAC AACTACTACA ACCACTGCTG CCGCCACCAC CACCACGGAG 
AGTCCTCCCA CCACCACCTC CGGGACTAAG ATACACGAAT CCGCCCCTGA TGAGCAGTCC 
ATATGGAACG TCACGGTGCT CCCCAACAGT AAATGGGCCA ACATCACCTG GAAGCACAAT 
TTCGGGCCCG GAACTGACTT TGTGGTTGAG TACATCGACA GCAACCATAC GAAAAAAACT 
GTCCCAGTTA AGGCCCAGGC TCAGCCTATA CAGCTGACAG ACCTCTATCC CGGGATGACA 
TACACGTTGC GGGTTTATTC CCGGGACAAC GAGGGCATCA GCAGTACCGT CATCACCTTT 
ATGACCAGTA CAGCTTACAC CAACAACCAA GCGGACATCG CCACCCAGGG CTGGTTCATT 
GGGCTTATGT GCGCCATCGC CCTCCTGGTG CTGATCCTGC TCATCGTCTG TTTCATCAAG 
AGGAGTCGCG GCGGCAAGTA CCCAGTACGA GAAAAGAAGG ATGTTCCCCT TGGCCCTGAA 
GACCCCAAGG AAGAGGATGG CTCATTTGAC TATAGTGATG AGGACAACAA GCCCCTGCAG 
GGCAGTCAGA CATCTCTGGA CGGCACCATC AAGCAGCAGG AGAGTGACGA CAGCCTGGTG 
GACTATGGCG AGGGTGGCGA GGGTCAGTTC AATGAAGACG GCTCCTTCAT CGGCCAGTAC 
ACGGTCAAAA AGGACAAGGA GGAAACAGAG GGCAACGAAA GCTCAGAGGC CACGTCACCT 
GTCAATGCTA TCTACTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Copy number variation, increased gene expression, and molecular mechanisms of neurofascin in lung cancer.
Molecular carcinogenesis56(9)2076-2085(2017 Sep)
Samulin Erdem J,Arnoldussen YJ,Skaug V,Haugen A,Zienolddiny S

Autoantibodies to nodal isoforms of neurofascin in chronic inflammatory demyelinating polyneuropathy.
Brain : a journal of neurology140(7)1851-1858(2017 Jul)
Delmont E,Manso C,Querol L,Cortese A,Berardinelli A,Lozza A,Belghazi M,Malissart P,Labauge P,Taieb G,Yuki N,Illa I,Attarian S,Devaux JJ

Neurofascin-155 IgG4 in chronic inflammatory demyelinating polyneuropathy.
Neurology86(9)800-7(2016 Mar)
Devaux JJ,Miura Y,Fukami Y,Inoue T,Manso C,Belghazi M,Sekiguchi K,Kokubun N,Ichikawa H,Wong AH,Yuki N

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NFASC cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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