NFASC cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFASC
Entrez Gene ID	512796
Full Name	neurofascin
General protein information	Preferred Names neurofascin Names neurofascin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015475023.1	XP_015330509.1	neurofascin isoform X18
XM_015475024.1	XP_015330510.1	neurofascin isoform X19
XM_005216707.3	XP_005216764.1	neurofascin isoform X6
XM_010813043.2	XP_010811345.1	neurofascin isoform X7
XM_005216710.3	XP_005216767.1	neurofascin isoform X9
XM_002693905.4	XP_002693951.2	neurofascin isoform X10
XM_003587045.4	XP_003587093.2	neurofascin isoform X13
XM_005216715.3	XP_005216772.1	neurofascin isoform X17
XM_015466692.1	XP_015322178.1	neurofascin isoform X4
XM_015466695.1	XP_015322181.1	neurofascin isoform X7
XM_015466700.1	XP_015322186.1	neurofascin isoform X12
XM_015466703.1	XP_015322189.1	neurofascin isoform X15
XM_024976633.1	XP_024832401.1	neurofascin isoform X1
XM_024976636.1	XP_024832404.1	neurofascin isoform X4
XM_024976638.1	XP_024832406.1	neurofascin isoform X6
XM_024976641.1	XP_024832409.1	neurofascin isoform X9
XM_024976642.1	XP_024832410.1	neurofascin isoform X10
XM_005216711.3	XP_005216768.1	neurofascin isoform X11
XM_005216703.3	XP_005216760.1	neurofascin isoform X1
XM_005216705.3	XP_005216762.1	neurofascin isoform X3
XM_015466693.1	XP_015322179.1	neurofascin isoform X5
XM_015466698.1	XP_015322184.1	neurofascin isoform X10
XM_015466705.1	XP_015322191.1	neurofascin isoform X17
XM_005216713.2	XP_005216770.1	neurofascin isoform X14
XM_024976635.1	XP_024832403.1	neurofascin isoform X3
XM_024976643.1	XP_024832411.1	neurofascin isoform X11
XM_024976644.1	XP_024832412.1	neurofascin isoform X12
XM_024976645.1	XP_024832413.1	neurofascin isoform X13
XM_005216713.3	XP_005216770.2	neurofascin isoform X2
XM_015466697.1	XP_015322183.1	neurofascin isoform X9
XM_015466699.1	XP_015322185.1	neurofascin isoform X11
XM_024976637.1	XP_024832405.1	neurofascin isoform X5
XM_024976649.1	XP_024832417.1	neurofascin isoform X17
XM_024976651.1	XP_024832419.1	neurofascin isoform X19
XM_015466707.1	XP_015322193.1	neurofascin isoform X18
XM_015466690.1	XP_015322176.1	neurofascin isoform X2
XM_015466701.1	XP_015322187.1	neurofascin isoform X13
XM_005216704.3	XP_005216761.1	neurofascin isoform X2
XM_005216706.3	XP_005216763.1	neurofascin isoform X4
XM_005216708.3	XP_005216765.1	neurofascin isoform X8
XM_002693906.4	XP_002693952.2	neurofascin isoform X15
XM_024976639.1	XP_024832407.1	neurofascin isoform X7
XM_024976646.1	XP_024832414.1	neurofascin isoform X14
XM_024976650.1	XP_024832418.1	neurofascin isoform X18
XM_015466689.1	XP_015322175.1	neurofascin isoform X1
XM_015466691.1	XP_015322177.1	neurofascin isoform X3
XM_015466694.1	XP_015322180.1	neurofascin isoform X6
XM_015466696.1	XP_015322182.1	neurofascin isoform X8
XM_015466704.1	XP_015322190.1	neurofascin isoform X16
XM_010813042.2	XP_010811344.1	neurofascin isoform X5
XM_005216712.3	XP_005216769.1	neurofascin isoform X12
XM_005216714.3	XP_005216771.1	neurofascin isoform X16
XM_015466702.1	XP_015322188.1	neurofascin isoform X14
XM_015466706.1	XP_015322192.1	neurofascin isoform X14
XM_015466708.1	XP_015322194.1	neurofascin isoform X19
XM_024976634.1	XP_024832402.1	neurofascin isoform X2
XM_024976640.1	XP_024832408.1	neurofascin isoform X8
XM_024976647.1	XP_024832415.1	neurofascin isoform X15
XM_024976648.1	XP_024832416.1	neurofascin isoform X16
XM_024976652.1	XP_024832420.1	neurofascin isoform X20

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nfasc	NP_874385.1
Xenopus tropicalis (tropical clawed frog)	nfasc	XP_004910755.1
Rattus norvegicus (Norway rat)	Nfasc	NP_001153786.1
Danio rerio (zebrafish)	nfasca	NP_001139239.1
	NFASC	XP_001096628.1
Canis lupus familiaris (dog)	NFASC	XP_005640834.1
Pan troglodytes (chimpanzee)	NFASC	XP_003308755.1
Gallus gallus (chicken)	NFASC	NP_001153794.1
Homo sapiens (human)	NFASC	NP_001005388.2
Bos taurus (cattle)	NFASC	XP_005216764.1

Gene Ontology

The following NFASC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFASC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa223836	XM_024976633.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223837	XM_005216713.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223837	XM_024976634.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223838	XM_024976635.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223839	XM_024976636.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223840	XM_024976637.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223841	XM_024976638.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223842	XM_024976639.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223843	XM_024976640.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223844	XM_024976641.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223845	XM_024976642.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223846	XM_024976643.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223847	XM_024976644.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223848	XM_024976645.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223849	XM_024976646.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223850	XM_024976647.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223851	XM_024976648.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223852	XM_024976649.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223853	XM_024976650.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223854	XM_024976651.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223855	XM_024976652.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa26984	XM_005216703.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26984	XM_015466689.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26985	XM_015466690.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26985	XM_005216704.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26986	XM_015466691.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26986	XM_005216705.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26987	XM_015466692.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26987	XM_005216706.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26988	XM_005216707.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26988	XM_015466694.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26989	XM_005216708.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26989	XM_015466696.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26990	XM_005216713.2 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26990	XM_015466706.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26990	XM_015466702.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26991	XM_005216710.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26991	XM_015466697.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26992	XM_015466698.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26992	XM_002693905.4 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26993	XM_005216711.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26993	XM_015466699.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26994	XM_005216712.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26994	XM_015466700.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26995	XM_015466701.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26995	XM_003587045.4 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26996	XM_015466703.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26996	XM_002693906.4 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26997	XM_015466704.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26997	XM_005216714.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26998	XM_015466705.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26998	XM_005216715.3 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa48428	XM_015466693.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa48428	XM_010813042.2 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa48429	XM_010813043.2 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa48429	XM_015466695.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa92881	XM_015466707.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa92881	XM_015475023.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa92882	XM_015475024.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa92882	XM_015466708.1 Latest version!	Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFASC ( XM_024976633.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832401.1 Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa223836
Clone ID Related Accession (Same CDS sequence)	XM_024976633.1
Accession Version	XM_024976633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG 
AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG 
CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG 
TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC 
AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC 
ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC 
AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC 
AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC 
GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832401.1
CDS	101..4501
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976633.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976633.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG 
AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG 
CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG 
TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC 
AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC 
ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC 
AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC 
AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC 
GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216713.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216770.2 Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

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CloneID	OBa223837
Clone ID Related Accession (Same CDS sequence)	XM_005216713.3 , XM_024976634.1
Accession Version	XM_005216713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCGC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216770.2
CDS	379..4461
Translation	MAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216713.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216713.3

ORF Insert Sequence:

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCGC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976634.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832402.1 Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa223837
Clone ID Related Accession (Same CDS sequence)	XM_005216713.3 , XM_024976634.1
Accession Version	XM_024976634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCGC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832402.1
CDS	304..4386
Translation	MAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976634.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976634.1

ORF Insert Sequence:

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCGC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976635.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832403.1 Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa223838
Clone ID Related Accession (Same CDS sequence)	XM_024976635.1
Accession Version	XM_024976635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832403.1
CDS	101..4483
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976635.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976635.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976636.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832404.1 Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

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CloneID	OBa223839
Clone ID Related Accession (Same CDS sequence)	XM_024976636.1
Accession Version	XM_024976636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC 
CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC 
AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG 
GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG 
CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC 
AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT 
GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC 
ACCTCGGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC 
GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC 
TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA 
GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA 
ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG 
GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG 
AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT 
GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG 
CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT 
GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT 
CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT 
GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG 
GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG 
GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC 
GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC 
GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC 
GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC 
ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC 
GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG 
AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC 
GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC 
ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC 
TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC 
GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC 
GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC 
ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832404.1
CDS	101..4456
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976636.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976636.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC 
CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC 
AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG 
GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG 
CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC 
AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT 
GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC 
ACCTCGGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC 
GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC 
TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA 
GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA 
ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG 
GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG 
AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT 
GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG 
CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT 
GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT 
CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT 
GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG 
GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG 
GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC 
GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC 
GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC 
GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC 
ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC 
GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG 
AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC 
GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC 
ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC 
TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC 
GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC 
GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC 
ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976637.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832405.1 Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

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CloneID	OBa223840
Clone ID Related Accession (Same CDS sequence)	XM_024976637.1
Accession Version	XM_024976637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA 
GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG 
GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG 
TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC 
GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC 
AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA 
GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC 
AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA 
AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA 
GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG 
CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC 
ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT 
ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC 
ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG 
TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC 
TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC 
ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT 
GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA 
CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832405.1
CDS	101..4450
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976637.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976637.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA 
GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG 
GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG 
TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC 
GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC 
AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA 
GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC 
AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA 
AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA 
GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG 
CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC 
ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT 
ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC 
ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG 
TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC 
TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC 
ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT 
GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA 
CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976638.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832406.1 Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa223841
Clone ID Related Accession (Same CDS sequence)	XM_024976638.1
Accession Version	XM_024976638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG 
AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC 
GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC 
CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG 
GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA 
ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG 
AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CGGCCTTCGG CCCCAACCTG 
CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG 
TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA 
GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT 
CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG 
CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA 
ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC 
CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG 
CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG 
GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG 
ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC 
ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC 
GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC 
ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT 
GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT 
TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC 
TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC 
ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG 
GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC 
CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC 
AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832406.1
CDS	101..4432
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976638.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976638.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG 
AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC 
GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC 
CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG 
GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA 
ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG 
AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CGGCCTTCGG CCCCAACCTG 
CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG 
TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA 
GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT 
CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG 
CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA 
ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC 
CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG 
CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG 
GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG 
ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC 
ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC 
GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC 
ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT 
GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT 
TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC 
TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC 
ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG 
GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC 
CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC 
AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976639.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832407.1 Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa223842
Clone ID Related Accession (Same CDS sequence)	XM_024976639.1
Accession Version	XM_024976639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG 
CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA 
AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC 
ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG 
TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC 
CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC 
CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG 
TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832407.1
CDS	101..4273
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976639.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976639.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG 
CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA 
AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC 
ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG 
TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC 
CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC 
CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG 
TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976640.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832408.1 Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

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CloneID	OBa223843
Clone ID Related Accession (Same CDS sequence)	XM_024976640.1
Accession Version	XM_024976640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC 
GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC 
GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG 
CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT 
GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG 
TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC 
GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA 
GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC 
GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG 
AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC 
ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832408.1
CDS	101..4258
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976640.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976640.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC 
GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC 
GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG 
CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT 
GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG 
TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC 
GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA 
GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC 
GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG 
AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC 
ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976641.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832409.1 Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa223844
Clone ID Related Accession (Same CDS sequence)	XM_024976641.1
Accession Version	XM_024976641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832409.1
CDS	101..4231
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976641.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976641.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976642.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832410.1 Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa223845
Clone ID Related Accession (Same CDS sequence)	XM_024976642.1
Accession Version	XM_024976642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC 
GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA 
ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC 
ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT 
GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT 
GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC 
GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC 
CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832410.1
CDS	101..4180
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976642.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976642.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC 
GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA 
ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC 
ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT 
GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT 
GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC 
GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC 
CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976643.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832411.1 Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

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CloneID	OBa223846
Clone ID Related Accession (Same CDS sequence)	XM_024976643.1
Accession Version	XM_024976643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC 
ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC 
GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA 
GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC 
TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG 
CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC 
TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT 
TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832411.1
CDS	101..4129
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976643.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976643.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC 
ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC 
GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA 
GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC 
TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG 
CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC 
TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT 
TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976644.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832412.1 Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

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CloneID	OBa223847
Clone ID Related Accession (Same CDS sequence)	XM_024976644.1
Accession Version	XM_024976644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832412.1
CDS	101..4003
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976644.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976644.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976645.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832413.1 Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa223848
Clone ID Related Accession (Same CDS sequence)	XM_024976645.1
Accession Version	XM_024976645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT 
GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG 
GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC 
GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG 
GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC 
GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG 
AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832413.1
CDS	101..3988
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976645.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976645.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT 
GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG 
GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC 
GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG 
GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC 
GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG 
AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976646.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832414.1 Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa223849
Clone ID Related Accession (Same CDS sequence)	XM_024976646.1
Accession Version	XM_024976646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832414.1
CDS	101..3985
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976646.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976646.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCGGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976647.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832415.1 Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

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CloneID	OBa223850
Clone ID Related Accession (Same CDS sequence)	XM_024976647.1
Accession Version	XM_024976647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCGGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832415.1
CDS	101..3940
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976647.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976647.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCGGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976648.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832416.1 Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa223851
Clone ID Related Accession (Same CDS sequence)	XM_024976648.1
Accession Version	XM_024976648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCGGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832416.1
CDS	101..3925
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976648.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976648.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCGGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976649.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832417.1 Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa223852
Clone ID Related Accession (Same CDS sequence)	XM_024976649.1
Accession Version	XM_024976649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC 
ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC 
CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT 
GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC 
AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA 
TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA 
GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCGGCC 
TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG 
AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC 
TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC 
ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC 
CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC 
CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC 
CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT 
GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG 
CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA 
CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA 
GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA 
GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG 
GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832417.1
CDS	101..3889
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976649.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976649.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC 
ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC 
CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT 
GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC 
AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA 
TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA 
GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCGGCC 
TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG 
AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC 
TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC 
ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC 
CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC 
CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC 
CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT 
GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG 
CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA 
CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA 
GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA 
GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG 
GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976650.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832418.1 Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

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CloneID	OBa223853
Clone ID Related Accession (Same CDS sequence)	XM_024976650.1
Accession Version	XM_024976650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC 
CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG 
CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC 
AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC 
TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA 
GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832418.1
CDS	101..3682
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976650.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976650.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC 
CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG 
CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC 
AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC 
TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA 
GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976651.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832419.1 Bos taurus neurofascin (NFASC), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa223854
Clone ID Related Accession (Same CDS sequence)	XM_024976651.1
Accession Version	XM_024976651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCTACAC CAACAATCAG GCAGACATTG CCACCCAGGG CTGGTTCATC 
GGACTCATGT GTGCCATCGC CCTGCTGGTG CTGATCCTGC TCATCGTCTG TTTCATCAAG 
AGGAGTCGCG GCGGCAAGTA CCCCGTGCGA GAAAAGAAGG ATGTGCCCCT CGGCCCTGAA 
GACCCCAAAG AAGAGGATGG CTCCTTTGAT TACAGTGACG AGGACAACAA GCCCCTGCAG 
GGCAGCCAGA CGTCCCTGGA CGGCACCATT AAGCAGCAGG AGAGCGACGA CAGCCTGGTG 
GACTATGGCG AGGGTGGCGA GGGCCAGTTC AACGAGGACG GCTCCTTCAT CGGCCAGTAC 
ACAGTCAAGA AGGACAAGGA GGAGACGGAA GGCAACGAGA GCTCAGAGGC CACGTCACCG 
GTCAACGCCA TCTATTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832419.1
CDS	101..3667
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976651.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976651.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC GGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCTACAC CAACAATCAG GCAGACATTG CCACCCAGGG CTGGTTCATC 
GGACTCATGT GTGCCATCGC CCTGCTGGTG CTGATCCTGC TCATCGTCTG TTTCATCAAG 
AGGAGTCGCG GCGGCAAGTA CCCCGTGCGA GAAAAGAAGG ATGTGCCCCT CGGCCCTGAA 
GACCCCAAAG AAGAGGATGG CTCCTTTGAT TACAGTGACG AGGACAACAA GCCCCTGCAG 
GGCAGCCAGA CGTCCCTGGA CGGCACCATT AAGCAGCAGG AGAGCGACGA CAGCCTGGTG 
GACTATGGCG AGGGTGGCGA GGGCCAGTTC AACGAGGACG GCTCCTTCAT CGGCCAGTAC 
ACAGTCAAGA AGGACAAGGA GGAGACGGAA GGCAACGAGA GCTCAGAGGC CACGTCACCG 
GTCAACGCCA TCTATTCTCT GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_024976652.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832420.1 Bos taurus neurofascin (NFASC), transcript variant X21, mRNA.

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CloneID	OBa223855
Clone ID Related Accession (Same CDS sequence)	XM_024976652.1
Accession Version	XM_024976652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofascin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832420.1
CDS	101..3631
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCALSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_024976652.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976652.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCA 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCGCGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCG 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216703.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216760.1 Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26984
Clone ID Related Accession (Same CDS sequence)	XM_005216703.3 , XM_015466689.1
Accession Version	XM_005216703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG 
AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG 
CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG 
TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC 
AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC 
ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC 
AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC 
AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC 
GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216760.1
CDS	72..4472
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216703.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216703.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG 
AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG 
CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG 
TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC 
AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC 
ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC 
AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC 
AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC 
GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466689.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322175.1 Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26984
Clone ID Related Accession (Same CDS sequence)	XM_005216703.3 , XM_015466689.1
Accession Version	XM_015466689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG 
AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG 
CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG 
TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC 
AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC 
ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC 
AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC 
AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC 
GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322175.1
CDS	72..4472
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466689.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466689.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCC CCGACGAGCA GTCCATCTGG 
AACGTCACTG TGCTCCCCAA CAGTAAATGG GCCAACATCA GCTGGAAGCA CAATTTCGGG 
CCCGGAACTG ACTTTGTGGT TGAGTACACC GACAGCAATC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC CGTACAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG 
TTGCGCGTTT ATTCCCGGGA CCACGAGGGC ATCAGCAGTG CCGTCATCAC CTTTATGACC 
AGTGCAGCCT ACACCAACAA TCAGGCAGAC ATTGCCACCC AGGGCTGGTT CATCGGACTC 
ATGTGTGCCA TCGCCCTGCT GGTGCTGATC CTGCTCATCG TCTGTTTCAT CAAGAGGAGT 
CGCGGCGGCA AGTACCCCGT GCGAGAAAAG AAGGATGTGC CCCTCGGCCC TGAAGACCCC 
AAAGAAGAGG ATGGCTCCTT TGATTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACGTCCC TGGACGGCAC CATTAAGCAG CAGGAGAGCG ACGACAGCCT GGTGGACTAT 
GGCGAGGGTG GCGAGGGCCA GTTCAACGAG GACGGCTCCT TCATCGGCCA GTACACAGTC 
AAGAAGGACA AGGAGGAGAC GGAAGGCAAC GAGAGCTCAG AGGCCACGTC ACCGGTCAAC 
GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466690.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322176.1 Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

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CloneID	OBa26985
Clone ID Related Accession (Same CDS sequence)	XM_015466690.1 , XM_005216704.3
Accession Version	XM_015466690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322176.1
CDS	72..4454
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466690.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466690.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216704.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216761.1 Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

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CloneID	OBa26985
Clone ID Related Accession (Same CDS sequence)	XM_015466690.1 , XM_005216704.3
Accession Version	XM_005216704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216761.1
CDS	72..4454
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216704.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216704.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216705.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216762.1 Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa26986
Clone ID Related Accession (Same CDS sequence)	XM_005216705.3 , XM_015466691.1
Accession Version	XM_005216705.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC 
CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC 
AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG 
GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG 
CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC 
AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT 
GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC 
ACCTCAGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC 
GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC 
TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA 
GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA 
ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG 
GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG 
AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT 
GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG 
CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT 
GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT 
CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT 
GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG 
GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG 
GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC 
GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC 
GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC 
GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC 
ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC 
GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG 
AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC 
GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC 
ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC 
TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC 
GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC 
GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC 
ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216762.1
CDS	72..4427
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216705.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216705.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC 
CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC 
AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG 
GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG 
CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC 
AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT 
GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC 
ACCTCAGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC 
GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC 
TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA 
GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA 
ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG 
GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG 
AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT 
GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG 
CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT 
GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT 
CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT 
GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG 
GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG 
GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC 
GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC 
GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC 
GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC 
ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC 
GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG 
AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC 
GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC 
ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC 
TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC 
GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC 
GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC 
ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466691.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322177.1 Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa26986
Clone ID Related Accession (Same CDS sequence)	XM_005216705.3 , XM_015466691.1
Accession Version	XM_015466691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC 
CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC 
AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG 
GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG 
CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC 
AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT 
GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC 
ACCTCAGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC 
GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC 
TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA 
GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA 
ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG 
GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG 
AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT 
GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG 
CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT 
GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT 
CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT 
GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG 
GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG 
GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC 
GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC 
GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC 
GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC 
ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC 
GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG 
AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC 
GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC 
ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC 
TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC 
GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC 
GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC 
ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322177.1
CDS	72..4427
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466691.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466691.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGGAC GACCGGACCG ACCCCGGGAC 
CTGGAGCTCA CCGACCTGGC TGAGAGAAGC GTGAGGCTGA CCTGGATCCC AGGGGACGAC 
AACAACAGCC CCATCACAGA CTACGTCGTC CAGTTTGAAG AAGACCAGTT CCAGCCAGGG 
GTCTGGCATG ACCATTCCAA GTTCCCGGGC AGTGTCAACT CAGCTGTGCT CCGGCTGTCG 
CCCTACGTCA ACTACCAGTT CCGGGTCATC GCCATCAATG AGGTGGGCAG CAGCCACCCC 
AGCCTCCCAT CTGAGCGCTA CCGAACCAGC GGAGCACCCC CCGAGTCCAA CCCCTCTGAT 
GTGAAGGGAG AGGGGACCAG GAAGAACAAC ATGGAGATCA CGTGGACGCC CATGAACGCC 
ACCTCAGCCT TCGGCCCCAA CCTGCGCTAC ATCGTCAAGT GGCGGCGGAG AGAGACCCGC 
GAGACCTGGA ACAATGTCAC CGTGTGGGGC TCCCGCTACG TGGTGGGGCA GACCCCCGTC 
TATGTGCCCT ACGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAGGG CCCTGAGCCA 
GACACTGTCA TCGGTTACTC TGGAGAAGAT TATCCCAGGG CTGCACCCAC AGATGTTAAA 
ATCCGAGTCC TGAACAGCAC CGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCCGACACG 
GTCCAGGGCC AGCTCAGAGA ATATCGAGCC TACTACTGGA GGGAGAGCAG CTTGCTGAAG 
AACCTGTGGG TGTCTCAGAA GAGGCAGCAA GCTGGCTTCC CTGGCGACCG CCTACGTGGT 
GTGGTGTCCC GCCTCTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG 
CGAGGCGATG GGCCTCGCAG TGAAACCAAG GAGTTCACCA CCCCGGAAGG AGTACCCAGT 
GCCCCCAGGC GTTTCCGAGT CCGGCAGCCC AACCTGGAGA CAATCAACCT GGAATGGGAT 
CATCCAGAAC ACCCCAATGG GATCCTGATT GGATATACTC TCAAATACGT GGCCTTTAAT 
GGAACCAAAG TAGGAAAGCA GATAGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCACG 
GTGCAAAGAG CAGACCCCGT GTCGCGCTAC CGCTTTTCCC TCAGTGCCAG GACGCAGGTG 
GGCTCAGGGG AAGCAGTCAC GGAGGAGTCG CCAGCACCCC CGAATGAAGC CACGCCAACC 
GCAGCGCCTC CCACGTCGCC CCCGACCACG GTGGGCGTGC CTGGCACGGT GAGCAGTACC 
GATGCCACTG CCACCGCCGC CGCCACCGAA GCCACAACAG TCCCCACCGT CCCACCTGTC 
GCACCTACCA CCACCGCCAC CACCGAAGCC ACAGCCACTA CAGCCTCCAC CGCCACCACC 
ACCACCACGG AGAGTCCCGC CACCACCTCC AGGACTGAGA TCCAAGAATC CGCCCCCGAC 
GAGCAGTCCA TCTGGAACGT CACTGTGCTC CCCAACAGTA AATGGGCCAA CATCAGCTGG 
AAGCACAATT TCGGGCCCGG AACTGACTTT GTGGTTGAGT ACACCGACAG CAATCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCCGTAC AGCTGACAGA CCTCTATCCC 
GGGATGACAT ACACGTTGCG CGTTTATTCC CGGGACCACG AGGGCATCAG CAGTGCCGTC 
ATCACCTTTA TGACCAGTGC AGCCTACACC AACAATCAGG CAGACATTGC CACCCAGGGC 
TGGTTCATCG GACTCATGTG TGCCATCGCC CTGCTGGTGC TGATCCTGCT CATCGTCTGT 
TTCATCAAGA GGAGTCGCGG CGGCAAGTAC CCCGTGCGAG AAAAGAAGGA TGTGCCCCTC 
GGCCCTGAAG ACCCCAAAGA AGAGGATGGC TCCTTTGATT ACAGTGACGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC GTCCCTGGAC GGCACCATTA AGCAGCAGGA GAGCGACGAC 
AGCCTGGTGG ACTATGGCGA GGGTGGCGAG GGCCAGTTCA ACGAGGACGG CTCCTTCATC 
GGCCAGTACA CAGTCAAGAA GGACAAGGAG GAGACGGAAG GCAACGAGAG CTCAGAGGCC 
ACGTCACCGG TCAACGCCAT CTATTCTCTG GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466692.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322178.1 Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

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CloneID	OBa26987
Clone ID Related Accession (Same CDS sequence)	XM_015466692.1 , XM_005216706.3
Accession Version	XM_015466692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA 
GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG 
GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG 
TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC 
GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC 
AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA 
GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC 
AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA 
AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA 
GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG 
CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC 
ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT 
ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC 
ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG 
TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC 
TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC 
ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT 
GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA 
CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322178.1
CDS	72..4421
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466692.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466692.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA 
GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG 
GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG 
TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC 
GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC 
AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA 
GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC 
AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA 
AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA 
GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG 
CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC 
ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT 
ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC 
ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG 
TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC 
TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC 
ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT 
GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA 
CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216706.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216763.1 Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

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CloneID	OBa26987
Clone ID Related Accession (Same CDS sequence)	XM_015466692.1 , XM_005216706.3
Accession Version	XM_005216706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA 
GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG 
GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG 
TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC 
GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC 
AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA 
GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC 
AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA 
AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA 
GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG 
CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC 
ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT 
ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC 
ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG 
TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC 
TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC 
ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT 
GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA 
CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216763.1
CDS	72..4421
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216706.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216706.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTATCCC AGGGCTGCAC CCACAGATGT TAAAATCCGA 
GTCCTGAACA GCACCGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG 
GGCCAGCTCA GAGAATATCG AGCCTACTAC TGGAGGGAGA GCAGCTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGGCA GCAAGCTGGC TTCCCTGGCG ACCGCCTACG TGGTGTGGTG 
TCCCGCCTCT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGCGAGGC 
GATGGGCCTC GCAGTGAAAC CAAGGAGTTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC 
AGGCGTTTCC GAGTCCGGCA GCCCAACCTG GAGACAATCA ACCTGGAATG GGATCATCCA 
GAACACCCCA ATGGGATCCT GATTGGATAT ACTCTCAAAT ACGTGGCCTT TAATGGAACC 
AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA 
AGAGCAGACC CCGTGTCGCG CTACCGCTTT TCCCTCAGTG CCAGGACGCA GGTGGGCTCA 
GGGGAAGCAG TCACGGAGGA GTCGCCAGCA CCCCCGAATG AAGCCACGCC AACCGCAGCG 
CCTCCCACGT CGCCCCCGAC CACGGTGGGC GTGCCTGGCA CGGTGAGCAG TACCGATGCC 
ACTGCCACCG CCGCCGCCAC CGAAGCCACA ACAGTCCCCA CCGTCCCACC TGTCGCACCT 
ACCACCACCG CCACCACCGA AGCCACAGCC ACTACAGCCT CCACCGCCAC CACCACCACC 
ACGGAGAGTC CCGCCACCAC CTCCAGGACT GAGATCCAAG AATCCGCCCC CGACGAGCAG 
TCCATCTGGA ACGTCACTGT GCTCCCCAAC AGTAAATGGG CCAACATCAG CTGGAAGCAC 
AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACACCG ACAGCAATCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC GTACAGCTGA CAGACCTCTA TCCCGGGATG 
ACATACACGT TGCGCGTTTA TTCCCGGGAC CACGAGGGCA TCAGCAGTGC CGTCATCACC 
TTTATGACCA GTGCAGCCTA CACCAACAAT CAGGCAGACA TTGCCACCCA GGGCTGGTTC 
ATCGGACTCA TGTGTGCCAT CGCCCTGCTG GTGCTGATCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GCGGCGGCAA GTACCCCGTG CGAGAAAAGA AGGATGTGCC CCTCGGCCCT 
GAAGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACGTCCCT GGACGGCACC ATTAAGCAGC AGGAGAGCGA CGACAGCCTG 
GTGGACTATG GCGAGGGTGG CGAGGGCCAG TTCAACGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AGAAGGACAA GGAGGAGACG GAAGGCAACG AGAGCTCAGA GGCCACGTCA 
CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216707.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216764.1 Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa26988
Clone ID Related Accession (Same CDS sequence)	XM_005216707.3 , XM_015466694.1
Accession Version	XM_005216707.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG 
CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA 
AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC 
ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG 
TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC 
CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC 
CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG 
TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216764.1
CDS	72..4244
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216707.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216707.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG 
CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA 
AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC 
ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG 
TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC 
CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC 
CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG 
TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466694.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322180.1 Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa26988
Clone ID Related Accession (Same CDS sequence)	XM_005216707.3 , XM_015466694.1
Accession Version	XM_015466694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG 
CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA 
AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC 
ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG 
TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC 
CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC 
CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG 
TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322180.1
CDS	72..4244
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466694.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466694.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CCCCGACGAG 
CAGTCCATCT GGAACGTCAC TGTGCTCCCC AACAGTAAAT GGGCCAACAT CAGCTGGAAG 
CACAATTTCG GGCCCGGAAC TGACTTTGTG GTTGAGTACA CCGACAGCAA TCATACGAAA 
AAAACTGTCC CTGTTAAGGC CCAGGCCCAG CCCGTACAGC TGACAGACCT CTATCCCGGG 
ATGACATACA CGTTGCGCGT TTATTCCCGG GACCACGAGG GCATCAGCAG TGCCGTCATC 
ACCTTTATGA CCAGTGCAGC CTACACCAAC AATCAGGCAG ACATTGCCAC CCAGGGCTGG 
TTCATCGGAC TCATGTGTGC CATCGCCCTG CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC 
ATCAAGAGGA GTCGCGGCGG CAAGTACCCC GTGCGAGAAA AGAAGGATGT GCCCCTCGGC 
CCTGAAGACC CCAAAGAAGA GGATGGCTCC TTTGATTACA GTGACGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACGTC CCTGGACGGC ACCATTAAGC AGCAGGAGAG CGACGACAGC 
CTGGTGGACT ATGGCGAGGG TGGCGAGGGC CAGTTCAACG AGGACGGCTC CTTCATCGGC 
CAGTACACAG TCAAGAAGGA CAAGGAGGAG ACGGAAGGCA ACGAGAGCTC AGAGGCCACG 
TCACCGGTCA ACGCCATCTA TTCTCTGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216708.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216765.1 Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

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CloneID	OBa26989
Clone ID Related Accession (Same CDS sequence)	XM_005216708.3 , XM_015466696.1
Accession Version	XM_005216708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216765.1
CDS	72..4202
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216708.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216708.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466696.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322182.1 Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa26989
Clone ID Related Accession (Same CDS sequence)	XM_005216708.3 , XM_015466696.1
Accession Version	XM_015466696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322182.1
CDS	72..4202
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466696.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466696.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC GCCTCCCACG 
TCGCCCCCGA CCACGGTGGG CGTGCCTGGC ACGGTGAGCA GTACCGATGC CACTGCCACC 
GCCGCCGCCA CCGAAGCCAC AACAGTCCCC ACCGTCCCAC CTGTCGCACC TACCACCACC 
GCCACCACCG AAGCCACAGC CACTACAGCC TCCACCGCCA CCACCACCAC CACGGAGAGT 
CCCGCCACCA CCTCCAGGAC TGAGATCCAA GAATCCGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216713.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216770.1 Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa26990
Clone ID Related Accession (Same CDS sequence)	XM_005216713.2 , XM_015466702.1 , XM_015466706.1
Accession Version	XM_005216713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005216713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216770.1
CDS	361..4443
Translation	MAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216713.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216713.2

ORF Insert Sequence:

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466702.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322188.1 Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

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CloneID	OBa26990
Clone ID Related Accession (Same CDS sequence)	XM_005216713.2 , XM_015466702.1 , XM_015466706.1
Accession Version	XM_015466702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322188.1
CDS	361..4443
Translation	MAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466702.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466702.1

ORF Insert Sequence:

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466706.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322192.1 Bos taurus neurofascin (NFASC), transcript variant X20, mRNA.

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CloneID	OBa26990
Clone ID Related Accession (Same CDS sequence)	XM_005216713.2 , XM_015466702.1 , XM_015466706.1
Accession Version	XM_015466706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322192.1
CDS	296..4378
Translation	MAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466706.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466706.1

ORF Insert Sequence:

ATGGCCGGGC AGCCGCCGCC CTGGGCCTAC ACAGCCGTCC TCCTCTGCAC GCTCAGCCTC 
GGCGGGGCCA TCGAGATCCC CATGGACCCA AGCATTCAGA ATGAGCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCGCCTCCCA CGTCGCCCCC GACCACGGTG 
GGCGTGCCTG GCACGGTGAG CAGTACCGAT GCCACTGCCA CCGCCGCCGC CACCGAAGCC 
ACAACAGTCC CCACCGTCCC ACCTGTCGCA CCTACCACCA CCGCCACCAC CGAAGCCACA 
GCCACTACAG CCTCCACCGC CACCACCACC ACCACGGAGA GTCCCGCCAC CACCTCCAGG 
ACTGAGATCC AAGAATCCGC CCCCGACGAG CAGTCCATCT GGAACGTCAC TGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CAGCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG 
GTTGAGTACA CCGACAGCAA TCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG 
GACCACGAGG GCATCAGCAG TGCCGTCATC ACCTTTATGA CCAGTGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216710.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216767.1 Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa26991
Clone ID Related Accession (Same CDS sequence)	XM_005216710.3 , XM_015466697.1
Accession Version	XM_005216710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC 
GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA 
ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC 
ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT 
GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT 
GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC 
GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC 
CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216767.1
CDS	72..4151
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216710.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216710.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC 
GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA 
ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC 
ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT 
GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT 
GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC 
GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC 
CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466697.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322183.1 Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa26991
Clone ID Related Accession (Same CDS sequence)	XM_005216710.3 , XM_015466697.1
Accession Version	XM_015466697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC 
GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA 
ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC 
ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT 
GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT 
GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC 
GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC 
CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322183.1
CDS	72..4151
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466697.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466697.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCG CCTCCCACGT CGCCCCCGAC CACGGTGGGC 
GTGCCTGGCA CGGTGAGCAG TACCGATGCC ACTGCCACCG CCGCCGCCAC CGAAGCCACA 
ACAGTCCCCA CCGTCCCACC TGTCGCACCT ACCACCACCG CCACCACCGA AGCCACAGCC 
ACTACAGCCT CCACCGCCAC CACCACCACC ACGGAGAGTC CCGCCACCAC CTCCAGGACT 
GAGATCCAAG AATCCGCCCC CGACGAGCAG TCCATCTGGA ACGTCACTGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAG CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT 
GAGTACACCG ACAGCAATCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCC 
GTACAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGCGTTTA TTCCCGGGAC 
CACGAGGGCA TCAGCAGTGC CGTCATCACC TTTATGACCA GTGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_002693905.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002693951.2 Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

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CloneID	OBa26992
Clone ID Related Accession (Same CDS sequence)	XM_002693905.4 , XM_015466698.1
Accession Version	XM_002693905.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002693905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC 
ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC 
GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA 
GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC 
TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG 
CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC 
TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT 
TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002693951.2
CDS	72..4100
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_002693905.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002693905.4

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC 
ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC 
GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA 
GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC 
TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG 
CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC 
TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT 
TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466698.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322184.1 Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa26992
Clone ID Related Accession (Same CDS sequence)	XM_002693905.4 , XM_015466698.1
Accession Version	XM_015466698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC 
ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC 
GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA 
GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC 
TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG 
CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC 
TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT 
TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322184.1
CDS	72..4100
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466698.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466698.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCGC CTCCCACGTC GCCCCCGACC 
ACGGTGGGCG TGCCTGGCAC GGTGAGCAGT ACCGATGCCA CTGCCACCGC CGCCGCCACC 
GAAGCCACAA CAGTCCCCAC CGTCCCACCT GTCGCACCTA CCACCACCGC CACCACCGAA 
GCCACAGCCA CTACAGCCTC CACCGCCACC ACCACCACCA CGGAGAGTCC CGCCACCACC 
TCCAGGACTG AGATCCAAGA ATCCGCCCCC GACGAGCAGT CCATCTGGAA CGTCACTGTG 
CTCCCCAACA GTAAATGGGC CAACATCAGC TGGAAGCACA ATTTCGGGCC CGGAACTGAC 
TTTGTGGTTG AGTACACCGA CAGCAATCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCCG TACAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGCGTTTAT 
TCCCGGGACC ACGAGGGCAT CAGCAGTGCC GTCATCACCT TTATGACCAG TGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216711.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216768.1 Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa26993
Clone ID Related Accession (Same CDS sequence)	XM_005216711.3 , XM_015466699.1
Accession Version	XM_005216711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216768.1
CDS	72..3974
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216711.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216711.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466699.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322185.1 Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

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CloneID	OBa26993
Clone ID Related Accession (Same CDS sequence)	XM_005216711.3 , XM_015466699.1
Accession Version	XM_015466699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322185.1
CDS	72..3974
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466699.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466699.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCACGC CAACCGCAGC CTACACCAAC 
AATCAGGCAG ACATTGCCAC CCAGGGCTGG TTCATCGGAC TCATGTGTGC CATCGCCCTG 
CTGGTGCTGA TCCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGCGGCGG CAAGTACCCC 
GTGCGAGAAA AGAAGGATGT GCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGATGGCTCC 
TTTGATTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTGGACGGC 
ACCATTAAGC AGCAGGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AGGACGGCTC CTTCATCGGC CAGTACACAG TCAAGAAGGA CAAGGAGGAG 
ACGGAAGGCA ACGAGAGCTC AGAGGCCACG TCACCGGTCA ACGCCATCTA TTCTCTGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466700.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322186.1 Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa26994
Clone ID Related Accession (Same CDS sequence)	XM_015466700.1 , XM_005216712.3
Accession Version	XM_015466700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT 
GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG 
GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC 
GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG 
GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC 
GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG 
AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322186.1
CDS	72..3959
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466700.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466700.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT 
GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG 
GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC 
GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG 
GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC 
GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG 
AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216712.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216769.1 Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa26994
Clone ID Related Accession (Same CDS sequence)	XM_015466700.1 , XM_005216712.3
Accession Version	XM_005216712.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT 
GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG 
GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC 
GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG 
GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC 
GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG 
AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216769.1
CDS	72..3959
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216712.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216712.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCTACA CCAACAATCA GGCAGACATT 
GCCACCCAGG GCTGGTTCAT CGGACTCATG TGTGCCATCG CCCTGCTGGT GCTGATCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG 
GATGTGCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC 
GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTGG ACGGCACCAT TAAGCAGCAG 
GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGCCAGTT CAACGAGGAC 
GGCTCCTTCA TCGGCCAGTA CACAGTCAAG AAGGACAAGG AGGAGACGGA AGGCAACGAG 
AGCTCAGAGG CCACGTCACC GGTCAACGCC ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_003587045.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587093.2 Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

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CloneID	OBa26995
Clone ID Related Accession (Same CDS sequence)	XM_003587045.4 , XM_015466701.1
Accession Version	XM_003587045.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587093.2
CDS	72..3956
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_003587045.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587045.4

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466701.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322187.1 Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa26995
Clone ID Related Accession (Same CDS sequence)	XM_003587045.4 , XM_015466701.1
Accession Version	XM_015466701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322187.1
CDS	72..3956
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466701.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466701.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTTCCC 
AAAGGACGAC CGGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCTGA GAGAAGCGTG 
AGGCTGACCT GGATCCCAGG GGACGACAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG 
TTTGAAGAAG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCGGGCAGT 
GTCAACTCAG CTGTGCTCCG GCTGTCGCCC TACGTCAACT ACCAGTTCCG GGTCATCGCC 
ATCAATGAGG TGGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA 
GCACCCCCCG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGGAA GAACAACATG 
GAGATCACGT GGACGCCCAT GAACGCCACC TCAGCCTTCG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGCGAG ACCTGGAACA ATGTCACCGT GTGGGGCTCC 
CGCTACGTGG TGGGGCAGAC CCCCGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGGGCTG CACCCACAGA TGTTAAAATC CGAGTCCTGA ACAGCACCGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAATA TCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCT 
GGCTTCCCTG GCGACCGCCT ACGTGGTGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGATTGGA 
TATACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCAGGGGAAG CAGTCACGGA GGAGTCGCCA 
GCACCCCCGA ATGAAGCCAC GCCAACCGCA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466703.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322189.1 Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa26996
Clone ID Related Accession (Same CDS sequence)	XM_015466703.1 , XM_002693906.4
Accession Version	XM_015466703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322189.1
CDS	72..3911
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466703.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466703.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_002693906.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002693952.2 Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

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CloneID	OBa26996
Clone ID Related Accession (Same CDS sequence)	XM_015466703.1 , XM_002693906.4
Accession Version	XM_002693906.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002693906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002693952.2
CDS	72..3911
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_002693906.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002693906.4

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCACGCCAA CCGCAGCCTA CACCAACAAT 
CAGGCAGACA TTGCCACCCA GGGCTGGTTC ATCGGACTCA TGTGTGCCAT CGCCCTGCTG 
GTGCTGATCC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GCGGCGGCAA GTACCCCGTG 
CGAGAAAAGA AGGATGTGCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA TGGCTCCTTT 
GATTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACGTCCCT GGACGGCACC 
ATTAAGCAGC AGGAGAGCGA CGACAGCCTG GTGGACTATG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAGG ACGGCTCCTT CATCGGCCAG TACACAGTCA AGAAGGACAA GGAGGAGACG 
GAAGGCAACG AGAGCTCAGA GGCCACGTCA CCGGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466704.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322190.1 Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa26997
Clone ID Related Accession (Same CDS sequence)	XM_015466704.1 , XM_005216714.3
Accession Version	XM_015466704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322190.1
CDS	72..3896
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466704.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466704.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216714.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216771.1 Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa26997
Clone ID Related Accession (Same CDS sequence)	XM_015466704.1 , XM_005216714.3
Accession Version	XM_005216714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216771.1
CDS	72..3896
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216714.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216714.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCAACCACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATGTAC AGTGCCCGAG GAGTTGCAGA GAGAACACCC 
AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGTCAGATGG TGCTGCGTGG CATGGACCTC 
CTACTGGAGT GCATCGCCTC TGGGGTCCCC ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTGC CATCCGACAA GGCCAAGTTT GAGAACTTCA ACAAGGCCCT GCGAATCACC 
AACGTGTCCG AGGAAGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGT 
ATCCGGCACA CGATCTCGGT GAGAGTGAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG 
AACCTCATCC TGGCTCCTGG CGAGGATGGG AGACTGGTGT GTCGAGCCAA TGGGAACCCC 
AAGCCCACGG TCCAGTGGAT GGTGAACGGG GAACCTTTGC AATCGGCACC GCCCAACCCC 
AACCGTGAGG TGGCCGGGGA CACCATCATC TTCCGTGACA CGCAGATCAG CAGCCGGGCC 
GTGTACCAGT GCAACACCTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC 
GTGCTGGACG TGCCGCCTCG GATGCTGTCG CCCCGGAACC AGCTCATCAG GGTGATCCTG 
TACAACCGGA CCCGGCTGGA CTGCCCATTC TTTGGGTCTC CCATCCCCAC GCTCCGATGG 
TTTAAGAATG GACAAGGAAG CAACCTGGAT GGTGGCAACT ACCACGTCTA TGAGAACGGC 
AGTCTGGAGA TCAAGATGAT CCGAAAAGAG GACCAGGGCA TCTACACCTG TGTTGCCACC 
AACATCCTGG GCAAAGCAGA GAACCAGGTC CGCCTCGAGG TCAAAGACCC CACGAGGATC 
TACCGGATGC CCGAGGACCA GGTCGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAGCACG ACCCCTCCCT GAAGCTCGCG GTCTCCTGGC TGAAGGACGA CGAGCCGCTC 
TACATCAGCA ACAGAATGAA GAAGGAGGAG GATTCCCTGA CCATCTTCGG CGTGGCCGAG 
CGGGACCAGG GCAGCTACAC GTGCGTTGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAA 
GCCTACCTGA CCGTCCTCGG ACGACCGGAC CGACCCCGGG ACCTGGAGCT CACCGACCTG 
GCTGAGAGAA GCGTGAGGCT GACCTGGATC CCAGGGGACG ACAACAACAG CCCCATCACA 
GACTACGTCG TCCAGTTTGA AGAAGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC 
AAGTTCCCGG GCAGTGTCAA CTCAGCTGTG CTCCGGCTGT CGCCCTACGT CAACTACCAG 
TTCCGGGTCA TCGCCATCAA TGAGGTGGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC 
TACCGAACCA GCGGAGCACC CCCCGAGTCC AACCCCTCTG ATGTGAAGGG AGAGGGGACC 
AGGAAGAACA ACATGGAGAT CACGTGGACG CCCATGAACG CCACCTCAGC CTTCGGCCCC 
AACCTGCGCT ACATCGTCAA GTGGCGGCGG AGAGAGACCC GCGAGACCTG GAACAATGTC 
ACCGTGTGGG GCTCCCGCTA CGTGGTGGGG CAGACCCCCG TCTATGTGCC CTACGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACTGT CATCGGTTAC 
TCTGGAGAAG ATTATCCCAG GGCTGCACCC ACAGATGTTA AAATCCGAGT CCTGAACAGC 
ACCGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCAGCTCAGA 
GAATATCGAG CCTACTACTG GAGGGAGAGC AGCTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGGCAGC AAGCTGGCTT CCCTGGCGAC CGCCTACGTG GTGTGGTGTC CCGCCTCTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGCGAGGCGA TGGGCCTCGC 
AGTGAAACCA AGGAGTTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCCGA 
GTCCGGCAGC CCAACCTGGA GACAATCAAC CTGGAATGGG ATCATCCAGA ACACCCCAAT 
GGGATCCTGA TTGGATATAC TCTCAAATAC GTGGCCTTTA ATGGAACCAA AGTAGGAAAG 
CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC 
GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC AGGACGCAGG TGGGCTCAGG GGAAGCAGTC 
ACGGAGGAGT CGCCAGCACC CCCGAATGAA GCCTACACCA ACAATCAGGC AGACATTGCC 
ACCCAGGGCT GGTTCATCGG ACTCATGTGT GCCATCGCCC TGCTGGTGCT GATCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGCGGC GGCAAGTACC CCGTGCGAGA AAAGAAGGAT 
GTGCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTGGACG GCACCATTAA GCAGCAGGAG 
AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GCCAGTTCAA CGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAGAAG GACAAGGAGG AGACGGAAGG CAACGAGAGC 
TCAGAGGCCA CGTCACCGGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_005216715.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216772.1 Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

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CloneID	OBa26998
Clone ID Related Accession (Same CDS sequence)	XM_005216715.3 , XM_015466705.1
Accession Version	XM_005216715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC 
ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC 
CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT 
GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC 
AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA 
TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA 
GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCAGCC 
TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG 
AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC 
TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC 
ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC 
CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC 
CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC 
CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT 
GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG 
CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA 
CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA 
GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA 
GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG 
GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216772.1
CDS	72..3860
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_005216715.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216715.3

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC 
ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC 
CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT 
GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC 
AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA 
TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA 
GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCAGCC 
TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG 
AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC 
TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC 
ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC 
CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC 
CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC 
CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT 
GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG 
CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA 
CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA 
GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA 
GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG 
GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466705.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322191.1 Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa26998
Clone ID Related Accession (Same CDS sequence)	XM_005216715.3 , XM_015466705.1
Accession Version	XM_015466705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC 
ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC 
CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT 
GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC 
AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA 
TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA 
GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCAGCC 
TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG 
AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC 
TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC 
ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC 
CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC 
CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC 
CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT 
GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG 
CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA 
CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA 
GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA 
GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG 
GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322191.1
CDS	72..3860
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466705.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466705.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGGA CGACCGGACC GACCCCGGGA CCTGGAGCTC 
ACCGACCTGG CTGAGAGAAG CGTGAGGCTG ACCTGGATCC CAGGGGACGA CAACAACAGC 
CCCATCACAG ACTACGTCGT CCAGTTTGAA GAAGACCAGT TCCAGCCAGG GGTCTGGCAT 
GACCATTCCA AGTTCCCGGG CAGTGTCAAC TCAGCTGTGC TCCGGCTGTC GCCCTACGTC 
AACTACCAGT TCCGGGTCAT CGCCATCAAT GAGGTGGGCA GCAGCCACCC CAGCCTCCCA 
TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCCGAGTCCA ACCCCTCTGA TGTGAAGGGA 
GAGGGGACCA GGAAGAACAA CATGGAGATC ACGTGGACGC CCATGAACGC CACCTCAGCC 
TTCGGCCCCA ACCTGCGCTA CATCGTCAAG TGGCGGCGGA GAGAGACCCG CGAGACCTGG 
AACAATGTCA CCGTGTGGGG CTCCCGCTAC GTGGTGGGGC AGACCCCCGT CTATGTGCCC 
TACGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACTGTC 
ATCGGTTACT CTGGAGAAGA TTATCCCAGG GCTGCACCCA CAGATGTTAA AATCCGAGTC 
CTGAACAGCA CCGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC 
CAGCTCAGAG AATATCGAGC CTACTACTGG AGGGAGAGCA GCTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGGCAGCA AGCTGGCTTC CCTGGCGACC GCCTACGTGG TGTGGTGTCC 
CGCCTCTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GCGAGGCGAT 
GGGCCTCGCA GTGAAACCAA GGAGTTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG 
CGTTTCCGAG TCCGGCAGCC CAACCTGGAG ACAATCAACC TGGAATGGGA TCATCCAGAA 
CACCCCAATG GGATCCTGAT TGGATATACT CTCAAATACG TGGCCTTTAA TGGAACCAAA 
GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA 
GCAGACCCCG TGTCGCGCTA CCGCTTTTCC CTCAGTGCCA GGACGCAGGT GGGCTCAGGG 
GAAGCAGTCA CGGAGGAGTC GCCAGCACCC CCGAATGAAG CCACGCCAAC CGCAGCCTAC 
ACCAACAATC AGGCAGACAT TGCCACCCAG GGCTGGTTCA TCGGACTCAT GTGTGCCATC 
GCCCTGCTGG TGCTGATCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG CGGCGGCAAG 
TACCCCGTGC GAGAAAAGAA GGATGTGCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACGTCCCTG 
GACGGCACCA TTAAGCAGCA GGAGAGCGAC GACAGCCTGG TGGACTATGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA GAAGGACAAG 
GAGGAGACGG AAGGCAACGA GAGCTCAGAG GCCACGTCAC CGGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466693.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322179.1 Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa48428
Clone ID Related Accession (Same CDS sequence)	XM_015466693.1 , XM_010813042.2
Accession Version	XM_015466693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG 
AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC 
GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC 
CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG 
GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA 
ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG 
AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CAGCCTTCGG CCCCAACCTG 
CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG 
TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA 
GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT 
CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG 
CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA 
ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC 
CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG 
CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG 
GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG 
ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC 
ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC 
GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC 
ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT 
GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT 
TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC 
TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC 
ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG 
GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC 
CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC 
AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322179.1
CDS	72..4403
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466693.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466693.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG 
AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC 
GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC 
CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG 
GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA 
ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG 
AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CAGCCTTCGG CCCCAACCTG 
CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG 
TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA 
GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT 
CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG 
CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA 
ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC 
CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG 
CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG 
GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG 
ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC 
ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC 
GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC 
ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT 
GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT 
TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC 
TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC 
ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG 
GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC 
CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC 
AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_010813042.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811344.1 Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

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CloneID	OBa48428
Clone ID Related Accession (Same CDS sequence)	XM_015466693.1 , XM_010813042.2
Accession Version	XM_010813042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG 
AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC 
GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC 
CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG 
GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA 
ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG 
AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CAGCCTTCGG CCCCAACCTG 
CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG 
TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA 
GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT 
CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG 
CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA 
ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC 
CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG 
CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG 
GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG 
ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC 
ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC 
GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC 
ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT 
GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT 
TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC 
TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC 
ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG 
GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC 
CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC 
AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811344.1
CDS	72..4403
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDLSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAPPTSPPTTVGVPGTVSSTDATATAAATEATTVPTVPPVAPTTTATTEATATTASTATTTTTESPATTSRTEIQESAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_010813042.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813042.2

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCTGTC CCAGCCCCCG 
ACCATCACCA AGCAGTCCGT GAAGGACCAC ATTGTGGATC CCCGTGACAA CATCTTGATT 
GAGTGTGAAG CCAAGGGGAA CCCTGCCCCC AGCTTCCACT GGACGCGCAA CAGCAAGTTC 
TTTAACATCG CCAAGGACCC CCGGGTGTCG ATGAGGAGGC GGTCCGGGAC GCTGGTGATC 
GACTTCCGCA GTGGCGGGCG GCCAGAGGAG TACGAGGGGG AATACCAGTG CTTTGCCCGC 
AACAAATTTG GCACGGCCCT GTCCAATCGG ATTCGCCTGC AGGTGTCCAA ATCCCCCCTG 
TGGCCCAAGG AAAACCTCGA CCCTGTCGTG GTTCAAGAAG GTGCCCCCTT GACGCTCCAG 
TGCAACCCCC CACCTGGGCT TCCATCCCCG GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AAGACAAACG TGTCTCCCAG GGCCATAATG GGGACCTGTA TTTCTCTAAT 
GTGATGCTGC AGGACATGCA GACAGACTAC AGCTGCAATG CCCGCTTCCA CTTCACCCAC 
ACCATCCAGC AGAAGAACCC ATTCACCCTC AAAGTCCTGA CCACCCGAGG AGTTGCAGAG 
AGAACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GTCAGATGGT GCTGCGTGGC 
ATGGACCTCC TACTGGAGTG CATCGCCTCT GGGGTCCCCA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTGCC ATCCGACAAG GCCAAGTTTG AGAACTTCAA CAAGGCCCTG 
CGAATCACCA ACGTGTCCGA GGAAGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGTA TCCGGCACAC GATCTCGGTG AGAGTGAAGG CTGCTCCCTA CTGGCTGGAT 
GAACCCAAGA ACCTCATCCT GGCTCCTGGC GAGGATGGGA GACTGGTGTG TCGAGCCAAT 
GGGAACCCCA AGCCCACGGT CCAGTGGATG GTGAACGGGG AACCTTTGCA ATCGGCACCG 
CCCAACCCCA ACCGTGAGGT GGCCGGGGAC ACCATCATCT TCCGTGACAC GCAGATCAGC 
AGCCGGGCCG TGTACCAGTG CAACACCTCC AACGAGCACG GCTACCTGCT GGCCAACGCC 
TTCGTCAGCG TGCTGGACGT GCCGCCTCGG ATGCTGTCGC CCCGGAACCA GCTCATCAGG 
GTGATCCTGT ACAACCGGAC CCGGCTGGAC TGCCCATTCT TTGGGTCTCC CATCCCCACG 
CTCCGATGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GTGGCAACTA CCACGTCTAT 
GAGAACGGCA GTCTGGAGAT CAAGATGATC CGAAAAGAGG ACCAGGGCAT CTACACCTGT 
GTTGCCACCA ACATCCTGGG CAAAGCAGAG AACCAGGTCC GCCTCGAGGT CAAAGACCCC 
ACGAGGATCT ACCGGATGCC CGAGGACCAG GTCGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAGCACGA CCCCTCCCTG AAGCTCGCGG TCTCCTGGCT GAAGGACGAC 
GAGCCGCTCT ACATCAGCAA CAGAATGAAG AAGGAGGAGG ATTCCCTGAC CATCTTCGGC 
GTGGCCGAGC GGGACCAGGG CAGCTACACG TGCGTTGCCA GCACTGAGCT GGACCAAGAC 
CTGGCCAAAG CCTACCTGAC CGTCCTCGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTTCCCA AAGGACGACC GGACCGACCC CGGGACCTGG AGCTCACCGA CCTGGCTGAG 
AGAAGCGTGA GGCTGACCTG GATCCCAGGG GACGACAACA ACAGCCCCAT CACAGACTAC 
GTCGTCCAGT TTGAAGAAGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC 
CCGGGCAGTG TCAACTCAGC TGTGCTCCGG CTGTCGCCCT ACGTCAACTA CCAGTTCCGG 
GTCATCGCCA TCAATGAGGT GGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA 
ACCAGCGGAG CACCCCCCGA GTCCAACCCC TCTGATGTGA AGGGAGAGGG GACCAGGAAG 
AACAACATGG AGATCACGTG GACGCCCATG AACGCCACCT CAGCCTTCGG CCCCAACCTG 
CGCTACATCG TCAAGTGGCG GCGGAGAGAG ACCCGCGAGA CCTGGAACAA TGTCACCGTG 
TGGGGCTCCC GCTACGTGGT GGGGCAGACC CCCGTCTATG TGCCCTACGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCTGGA 
GAAGATTATC CCAGGGCTGC ACCCACAGAT GTTAAAATCC GAGTCCTGAA CAGCACCGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCAGCT CAGAGAATAT 
CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGG 
CAGCAAGCTG GCTTCCCTGG CGACCGCCTA CGTGGTGTGG TGTCCCGCCT CTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGCGAG GCGATGGGCC TCGCAGTGAA 
ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CCGAGTCCGG 
CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CAGAACACCC CAATGGGATC 
CTGATTGGAT ATACTCTCAA ATACGTGGCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG 
CGCTACCGCT TTTCCCTCAG TGCCAGGACG CAGGTGGGCT CAGGGGAAGC AGTCACGGAG 
GAGTCGCCAG CACCCCCGAA TGAAGCCACG CCAACCGCAG CGCCTCCCAC GTCGCCCCCG 
ACCACGGTGG GCGTGCCTGG CACGGTGAGC AGTACCGATG CCACTGCCAC CGCCGCCGCC 
ACCGAAGCCA CAACAGTCCC CACCGTCCCA CCTGTCGCAC CTACCACCAC CGCCACCACC 
GAAGCCACAG CCACTACAGC CTCCACCGCC ACCACCACCA CCACGGAGAG TCCCGCCACC 
ACCTCCAGGA CTGAGATCCA AGAATCCGCC CCCGACGAGC AGTCCATCTG GAACGTCACT 
GTGCTCCCCA ACAGTAAATG GGCCAACATC AGCTGGAAGC ACAATTTCGG GCCCGGAACT 
GACTTTGTGG TTGAGTACAC CGACAGCAAT CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT 
TATTCCCGGG ACCACGAGGG CATCAGCAGT GCCGTCATCA CCTTTATGAC CAGTGCAGCC 
TACACCAACA ATCAGGCAGA CATTGCCACC CAGGGCTGGT TCATCGGACT CATGTGTGCC 
ATCGCCCTGC TGGTGCTGAT CCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC 
AAGTACCCCG TGCGAGAAAA GAAGGATGTG CCCCTCGGCC CTGAAGACCC CAAAGAAGAG 
GATGGCTCCT TTGATTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC 
CTGGACGGCA CCATTAAGCA GCAGGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT 
GGCGAGGGCC AGTTCAACGA GGACGGCTCC TTCATCGGCC AGTACACAGT CAAGAAGGAC 
AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA GAGGCCACGT CACCGGTCAA CGCCATCTAT 
TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_010813043.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811345.1 Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa48429
Clone ID Related Accession (Same CDS sequence)	XM_010813043.2 , XM_015466695.1
Accession Version	XM_010813043.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC 
GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC 
GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG 
CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT 
GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG 
TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC 
GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA 
GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC 
GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG 
AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC 
ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811345.1
CDS	72..4229
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_010813043.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813043.2

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC 
GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC 
GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG 
CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT 
GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG 
TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC 
GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA 
GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC 
GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG 
AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC 
ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466695.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322181.1 Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa48429
Clone ID Related Accession (Same CDS sequence)	XM_010813043.2 , XM_015466695.1
Accession Version	XM_015466695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC 
GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC 
GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG 
CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT 
GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG 
TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC 
GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA 
GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC 
GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG 
AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC 
ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322181.1
CDS	72..4229
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAPDEQSIWNVTVLPNSKWANISWKHNFGPGTDFVVEYTDSNHTKKTVPVKAQAQPVQLTDLYPGMTYTLRVYSRDHEGISSAVITFMTSAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466695.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466695.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTATCC CAGGGCTGCA CCCACAGATG TTAAAATCCG AGTCCTGAAC 
AGCACCGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC 
AGAGAATATC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGGC AGCAAGCTGG CTTCCCTGGC GACCGCCTAC GTGGTGTGGT GTCCCGCCTC 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGCGAGG CGATGGGCCT 
CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC AGAACACCCC 
AATGGGATCC TGATTGGATA TACTCTCAAA TACGTGGCCT TTAATGGAAC CAAAGTAGGA 
AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC 
CCCGTGTCGC GCTACCGCTT TTCCCTCAGT GCCAGGACGC AGGTGGGCTC AGGGGAAGCA 
GTCACGGAGG AGTCGCCAGC ACCCCCGAAT GAAGCCCCCG ACGAGCAGTC CATCTGGAAC 
GTCACTGTGC TCCCCAACAG TAAATGGGCC AACATCAGCT GGAAGCACAA TTTCGGGCCC 
GGAACTGACT TTGTGGTTGA GTACACCGAC AGCAATCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAGG CCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG 
CGCGTTTATT CCCGGGACCA CGAGGGCATC AGCAGTGCCG TCATCACCTT TATGACCAGT 
GCAGCCTACA CCAACAATCA GGCAGACATT GCCACCCAGG GCTGGTTCAT CGGACTCATG 
TGTGCCATCG CCCTGCTGGT GCTGATCCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGC 
GGCGGCAAGT ACCCCGTGCG AGAAAAGAAG GATGTGCCCC TCGGCCCTGA AGACCCCAAA 
GAAGAGGATG GCTCCTTTGA TTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACGTCCCTGG ACGGCACCAT TAAGCAGCAG GAGAGCGACG ACAGCCTGGT GGACTATGGC 
GAGGGTGGCG AGGGCCAGTT CAACGAGGAC GGCTCCTTCA TCGGCCAGTA CACAGTCAAG 
AAGGACAAGG AGGAGACGGA AGGCAACGAG AGCTCAGAGG CCACGTCACC GGTCAACGCC 
ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015475023.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330509.1 Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

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CloneID	OBa92881
Clone ID Related Accession (Same CDS sequence)	XM_015475023.1 , XM_015466707.1
Accession Version	XM_015475023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC 
CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG 
CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC 
AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC 
TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA 
GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330509.1
CDS	72..3653
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015475023.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475023.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC 
CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG 
CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC 
AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC 
TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA 
GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466707.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322193.1 Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

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CloneID	OBa92881
Clone ID Related Accession (Same CDS sequence)	XM_015475023.1 , XM_015466707.1
Accession Version	XM_015466707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC 
CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG 
CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC 
AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC 
TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA 
GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322193.1
CDS	72..3653
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466707.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466707.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
AACCACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATGTACAG TGCCCGAGGA 
GTTGCAGAGA GAACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG TCAGATGGTG 
CTGCGTGGCA TGGACCTCCT ACTGGAGTGC ATCGCCTCTG GGGTCCCCAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTGCCA TCCGACAAGG CCAAGTTTGA GAACTTCAAC 
AAGGCCCTGC GAATCACCAA CGTGTCCGAG GAAGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGTAT CCGGCACACG ATCTCGGTGA GAGTGAAGGC TGCTCCCTAC 
TGGCTGGATG AACCCAAGAA CCTCATCCTG GCTCCTGGCG AGGATGGGAG ACTGGTGTGT 
CGAGCCAATG GGAACCCCAA GCCCACGGTC CAGTGGATGG TGAACGGGGA ACCTTTGCAA 
TCGGCACCGC CCAACCCCAA CCGTGAGGTG GCCGGGGACA CCATCATCTT CCGTGACACG 
CAGATCAGCA GCCGGGCCGT GTACCAGTGC AACACCTCCA ACGAGCACGG CTACCTGCTG 
GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCGCCTCGGA TGCTGTCGCC CCGGAACCAG 
CTCATCAGGG TGATCCTGTA CAACCGGACC CGGCTGGACT GCCCATTCTT TGGGTCTCCC 
ATCCCCACGC TCCGATGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG TGGCAACTAC 
CACGTCTATG AGAACGGCAG TCTGGAGATC AAGATGATCC GAAAAGAGGA CCAGGGCATC 
TACACCTGTG TTGCCACCAA CATCCTGGGC AAAGCAGAGA ACCAGGTCCG CCTCGAGGTC 
AAAGACCCCA CGAGGATCTA CCGGATGCCC GAGGACCAGG TCGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAGCACGAC CCCTCCCTGA AGCTCGCGGT CTCCTGGCTG 
AAGGACGACG AGCCGCTCTA CATCAGCAAC AGAATGAAGA AGGAGGAGGA TTCCCTGACC 
ATCTTCGGCG TGGCCGAGCG GGACCAGGGC AGCTACACGT GCGTTGCCAG CACTGAGCTG 
GACCAAGACC TGGCCAAAGC CTACCTGACC GTCCTCGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTTCCCAA AGGACGACCG GACCGACCCC GGGACCTGGA GCTCACCGAC 
CTGGCTGAGA GAAGCGTGAG GCTGACCTGG ATCCCAGGGG ACGACAACAA CAGCCCCATC 
ACAGACTACG TCGTCCAGTT TGAAGAAGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT 
TCCAAGTTCC CGGGCAGTGT CAACTCAGCT GTGCTCCGGC TGTCGCCCTA CGTCAACTAC 
CAGTTCCGGG TCATCGCCAT CAATGAGGTG GGCAGCAGCC ACCCCAGCCT CCCATCTGAG 
CGCTACCGAA CCAGCGGAGC ACCCCCCGAG TCCAACCCCT CTGATGTGAA GGGAGAGGGG 
ACCAGGAAGA ACAACATGGA GATCACGTGG ACGCCCATGA ACGCCACCTC AGCCTTCGGC 
CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAGA CCCGCGAGAC CTGGAACAAT 
GTCACCGTGT GGGGCTCCCG CTACGTGGTG GGGCAGACCC CCGTCTATGT GCCCTACGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT 
TACTCTGGAG AAGATTTACC CAGTGCCCCC AGGCGTTTCC GAGTCCGGCA GCCCAACCTG 
GAGACAATCA ACCTGGAATG GGATCATCCA GAACACCCCA ATGGGATCCT GATTGGATAT 
ACTCTCAAAT ACGTGGCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCA GGGGAAGCAG TCACGGAGGA GTCGCCAGCA 
CCCCCGAATG AAGCCACGCC AACCGCAGCC TACACCAACA ATCAGGCAGA CATTGCCACC 
CAGGGCTGGT TCATCGGACT CATGTGTGCC ATCGCCCTGC TGGTGCTGAT CCTGCTCATC 
GTCTGTTTCA TCAAGAGGAG TCGCGGCGGC AAGTACCCCG TGCGAGAAAA GAAGGATGTG 
CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GATGGCTCCT TTGATTACAG TGACGAGGAC 
AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTGGACGGCA CCATTAAGCA GCAGGAGAGC 
GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGCC AGTTCAACGA GGACGGCTCC 
TTCATCGGCC AGTACACAGT CAAGAAGGAC AAGGAGGAGA CGGAAGGCAA CGAGAGCTCA 
GAGGCCACGT CACCGGTCAA CGCCATCTAT TCTCTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015475024.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330510.1 Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa92882
Clone ID Related Accession (Same CDS sequence)	XM_015475024.1 , XM_015466708.1
Accession Version	XM_015475024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330510.1
CDS	72..3602
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015475024.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475024.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015466708.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322194.1 Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

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CloneID	OBa92882
Clone ID Related Accession (Same CDS sequence)	XM_015475024.1 , XM_015466708.1
Accession Version	XM_015466708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322194.1
CDS	72..3602
Translation	MVKICLECQSEPEERGPGSALRELLTDGETASTDTCSGARLTDFCAVWDAGVFLVSAAGKGAPSGVKKRRLNEAETLNSVREAQFQRLEAARPRGLGRGARGPVLRMAGQPPPWAYTAVLLCTLSLGGAIEIPMDPSIQNELSQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSKFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLAVSWLKDDEPLYISNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015466708.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofascin (NFASC), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466708.1

ORF Insert Sequence:

ATGGTCAAAA TATGCTTAGA ATGTCAGAGT GAGCCAGAGG AAAGAGGCCC AGGATCTGCC 
CTCAGGGAGC TCCTAACTGA TGGAGAGACA GCGAGTACAG ATACGTGCTC AGGAGCAAGG 
TTGACTGACT TCTGTGCAGT TTGGGACGCC GGAGTTTTCC TTGTCTCTGC AGCCGGGAAG 
GGAGCCCCCT CTGGCGTTAA GAAAAGGAGG CTGAATGAGG CAGAGACGCT GAACTCCGTC 
CGGGAGGCTC AGTTCCAGCG ACTGGAGGCA GCGAGACCTC GCGGGCTGGG GCGCGGGGCG 
CGAGGCCCGG TGCTCAGGAT GGCCGGGCAG CCGCCGCCCT GGGCCTACAC AGCCGTCCTC 
CTCTGCACGC TCAGCCTCGG CGGGGCCATC GAGATCCCCA TGGACCCAAG CATTCAGAAT 
GAGCTGTCCC AGCCCCCGAC CATCACCAAG CAGTCCGTGA AGGACCACAT TGTGGATCCC 
CGTGACAACA TCTTGATTGA GTGTGAAGCC AAGGGGAACC CTGCCCCCAG CTTCCACTGG 
ACGCGCAACA GCAAGTTCTT TAACATCGCC AAGGACCCCC GGGTGTCGAT GAGGAGGCGG 
TCCGGGACGC TGGTGATCGA CTTCCGCAGT GGCGGGCGGC CAGAGGAGTA CGAGGGGGAA 
TACCAGTGCT TTGCCCGCAA CAAATTTGGC ACGGCCCTGT CCAATCGGAT TCGCCTGCAG 
GTGTCCAAAT CCCCCCTGTG GCCCAAGGAA AACCTCGACC CTGTCGTGGT TCAAGAAGGT 
GCCCCCTTGA CGCTCCAGTG CAACCCCCCA CCTGGGCTTC CATCCCCGGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAA GACAAACGTG TCTCCCAGGG CCATAATGGG 
GACCTGTATT TCTCTAATGT GATGCTGCAG GACATGCAGA CAGACTACAG CTGCAATGCC 
CGCTTCCACT TCACCCACAC CATCCAGCAG AAGAACCCAT TCACCCTCAA AGTCCTGACC 
ACCCGAGGAG TTGCAGAGAG AACACCCAGC TTCATGTATC CCCAGGGCAC TGCGAGCAGT 
CAGATGGTGC TGCGTGGCAT GGACCTCCTA CTGGAGTGCA TCGCCTCTGG GGTCCCCACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTGCCAT CCGACAAGGC CAAGTTTGAG 
AACTTCAACA AGGCCCTGCG AATCACCAAC GTGTCCGAGG AAGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGTATC CGGCACACGA TCTCGGTGAG AGTGAAGGCT 
GCTCCCTACT GGCTGGATGA ACCCAAGAAC CTCATCCTGG CTCCTGGCGA GGATGGGAGA 
CTGGTGTGTC GAGCCAATGG GAACCCCAAG CCCACGGTCC AGTGGATGGT GAACGGGGAA 
CCTTTGCAAT CGGCACCGCC CAACCCCAAC CGTGAGGTGG CCGGGGACAC CATCATCTTC 
CGTGACACGC AGATCAGCAG CCGGGCCGTG TACCAGTGCA ACACCTCCAA CGAGCACGGC 
TACCTGCTGG CCAACGCCTT CGTCAGCGTG CTGGACGTGC CGCCTCGGAT GCTGTCGCCC 
CGGAACCAGC TCATCAGGGT GATCCTGTAC AACCGGACCC GGCTGGACTG CCCATTCTTT 
GGGTCTCCCA TCCCCACGCT CCGATGGTTT AAGAATGGAC AAGGAAGCAA CCTGGATGGT 
GGCAACTACC ACGTCTATGA GAACGGCAGT CTGGAGATCA AGATGATCCG AAAAGAGGAC 
CAGGGCATCT ACACCTGTGT TGCCACCAAC ATCCTGGGCA AAGCAGAGAA CCAGGTCCGC 
CTCGAGGTCA AAGACCCCAC GAGGATCTAC CGGATGCCCG AGGACCAGGT CGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAGCACGACC CCTCCCTGAA GCTCGCGGTC 
TCCTGGCTGA AGGACGACGA GCCGCTCTAC ATCAGCAACA GAATGAAGAA GGAGGAGGAT 
TCCCTGACCA TCTTCGGCGT GGCCGAGCGG GACCAGGGCA GCTACACGTG CGTTGCCAGC 
ACTGAGCTGG ACCAAGACCT GGCCAAAGCC TACCTGACCG TCCTCGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTTCCCAAA GGACGACCGG ACCGACCCCG GGACCTGGAG 
CTCACCGACC TGGCTGAGAG AAGCGTGAGG CTGACCTGGA TCCCAGGGGA CGACAACAAC 
AGCCCCATCA CAGACTACGT CGTCCAGTTT GAAGAAGACC AGTTCCAGCC AGGGGTCTGG 
CATGACCATT CCAAGTTCCC GGGCAGTGTC AACTCAGCTG TGCTCCGGCT GTCGCCCTAC 
GTCAACTACC AGTTCCGGGT CATCGCCATC AATGAGGTGG GCAGCAGCCA CCCCAGCCTC 
CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCCGAGT CCAACCCCTC TGATGTGAAG 
GGAGAGGGGA CCAGGAAGAA CAACATGGAG ATCACGTGGA CGCCCATGAA CGCCACCTCA 
GCCTTCGGCC CCAACCTGCG CTACATCGTC AAGTGGCGGC GGAGAGAGAC CCGCGAGACC 
TGGAACAATG TCACCGTGTG GGGCTCCCGC TACGTGGTGG GGCAGACCCC CGTCTATGTG 
CCCTACGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACT 
GTCATCGGTT ACTCTGGAGA AGATTTACCC AGTGCCCCCA GGCGTTTCCG AGTCCGGCAG 
CCCAACCTGG AGACAATCAA CCTGGAATGG GATCATCCAG AACACCCCAA TGGGATCCTG 
ATTGGATATA CTCTCAAATA CGTGGCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC 
TACCGCTTTT CCCTCAGTGC CAGGACGCAG GTGGGCTCAG GGGAAGCAGT CACGGAGGAG 
TCGCCAGCAC CCCCGAATGA AGCCACGCCA ACCGCAGCCT ACACCAACAA TCAGGCAGAC 
ATTGCCACCC AGGGCTGGTT CATCGGACTC ATGTGTGCCA TCGCCCTGCT GGTGCTGATC 
CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGCGGCGGCA AGTACCCCGT GCGAGAAAAG 
AAGGATGTGC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ATGGCTCCTT TGATTACAGT 
GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TGGACGGCAC CATTAAGCAG 
CAGGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGCCA GTTCAACGAG 
GACGGCTCCT TCATCGGCCA GTACACAGTC AAGAAGGACA AGGAGGAGAC GGAAGGCAAC 
GAGAGCTCAG AGGCCACGTC ACCGGTCAAC GCCATCTATT CTCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

NFASC cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: