MAGED1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAGED1
Entrez Gene ID	512562
Full Name	melanoma antigen family D, 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015461705.2	XP_015317191.2	melanoma-associated antigen D1 isoform X1
XM_005228193.3	XP_005228250.1	melanoma-associated antigen D1 isoform X2
XM_005228193.2	XP_005228250.1	melanoma-associated antigen D1 isoform X1
XM_015461705.1	XP_015317191.1	melanoma-associated antigen D1 isoform X1
XM_024987905.1	XP_024843673.1	melanoma-associated antigen D1 isoform X2
XM_024987906.1	XP_024843674.1	melanoma-associated antigen D1 isoform X2
NM_001046125.2	NP_001039590.2	melanoma-associated antigen D1

Pathways from BioSystems

Homologies

	LOC696658	XP_002808576.1
Mus musculus (house mouse)	Maged1	NP_062765.1
Rattus norvegicus (Norway rat)	Maged1	NP_445861.1
Canis lupus familiaris (dog)	MAGED1	XP_005641419.1
Bos taurus (cattle)	MAGED1	NP_001039590.2
Homo sapiens (human)	MAGED1	NP_001005333.1
Pan troglodytes (chimpanzee)	MAGED1	XP_003317513.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAGED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238336	XM_015461705.2 Latest version!	Bos taurus MAGE family member D1 (MAGED1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa35615	NM_001046125.2 Latest version!	Bos taurus MAGE family member D1 (MAGED1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa35615	XM_024987906.1 Latest version!	Bos taurus MAGE family member D1 (MAGED1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa35615	XM_005228193.3 Latest version!	Bos taurus MAGE family member D1 (MAGED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa35615	XM_024987905.1 Latest version!	Bos taurus MAGE family member D1 (MAGED1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa35615	XM_015461705.1 Latest version!	Bos taurus melanoma antigen family D, 1 (MAGED1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OBa35615	XM_005228193.2	Bos taurus melanoma antigen family D, 1 (MAGED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAGED1 ( XM_015461705.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317191.2 Bos taurus MAGE family member D1 (MAGED1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa238336
Clone ID Related Accession (Same CDS sequence)	XM_015461705.2
Accession Version	XM_015461705.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461705.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTGG GCTTCCCTGG TGGCTCACAT GGCTCTGCTC TTGCCAGAGA GACAAGAATC 
ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317191.2
CDS	62..2479
Translation	MALGFPGGSHGSALARETRIMSEKMDCGAGLLGFQAEASVEDSTLLMQTLMEAIQISEAPPTSQATAAASAPNASPQSSQPPAVSEMADTQALATATKPKTAFKVQNATTKGPNAAYDFSQAFNAKETPNILPTAHFKSQNAPAKGPNAAYDFSQAAPTGELTANKSDMAFKAQNTTTKVVPNAAYNFSQSLNASEMVNPQPKTAFKAWNDTTTAPTADPQTQNVNPAKMATSHTDIEADPSPGICESDGAAAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEESSSGDQRRAPLAPGTWRSAPVPVTTQSPPGAPPNVLWQTPLAWQNPSGWQNQPTRQTPPARQSPPTRQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPADWPLPPDWIPTDWPVPPDWQNLRPSPNLRPSPNSRASQNLGASQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	XM_015461705.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MAGE family member D1 (MAGED1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461705.2

ORF Insert Sequence:

ATGGCTTTGG GCTTCCCTGG TGGCTCACAT GGCTCTGCTC TTGCCAGAGA GACAAGAATC 
ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED1 ( NM_001046125.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001039590.2 Bos taurus MAGE family member D1 (MAGED1), mRNA.

Price & Availability↑

CloneID	OBa35615
Clone ID Related Accession (Same CDS sequence)	XM_005228193.3 , XM_005228193.2 , XM_015461705.1 , XM_024987905.1 , XM_024987906.1 , NM_001046125.2
Accession Version	NM_001046125.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-02
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EE227115.1, EE231198.1, DY221014.1, DT857798.1 and BC105492.1. On Jan 17, 2008 this sequence version replaced NM_001046125.1. ##Evidence-Data-START## CDS exon combination :: BC105492.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039590.2
CDS	162..2519
Translation	MSEKMDCGAGLLGFQAEASVEDSTLLMQTLMEAIQISEAPPTSQATAAASAPNASPQSSQPPAVSEMADTQALATATKPKTAFKVQNATTKGPNAAYDFSQAFNAKETPNILPTAHFKSQNAPAKGPNAAYDFSQAAPTGELTANKSDMAFKAQNTTTKVVPNAAYNFSQSLNASEMVNPQPKTAFKAWNDTTTAPTADPQTQNVNPAKMATSHTDIEADPSPGICESDGAAAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEESSSGDQRRAPLAPGTWRSAPVPVTTQSPPGAPPNVLWQTPLAWQNPSGWQNQPTRQTPPARQSPPTRQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPADWPLPPDWIPTDWPVPPDWQNLRPSPNLRPSPNSRASQNLGASQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	NM_001046125.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MAGE family member D1 (MAGED1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001046125.2

ORF Insert Sequence:

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED1 ( XM_005228193.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228250.1 Bos taurus MAGE family member D1 (MAGED1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35615
Clone ID Related Accession (Same CDS sequence)	XM_005228193.3 , XM_005228193.2 , XM_015461705.1 , XM_024987905.1 , XM_024987906.1 , NM_001046125.2
Accession Version	XM_005228193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228250.1
CDS	278..2635
Translation	MSEKMDCGAGLLGFQAEASVEDSTLLMQTLMEAIQISEAPPTSQATAAASAPNASPQSSQPPAVSEMADTQALATATKPKTAFKVQNATTKGPNAAYDFSQAFNAKETPNILPTAHFKSQNAPAKGPNAAYDFSQAAPTGELTANKSDMAFKAQNTTTKVVPNAAYNFSQSLNASEMVNPQPKTAFKAWNDTTTAPTADPQTQNVNPAKMATSHTDIEADPSPGICESDGAAAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEESSSGDQRRAPLAPGTWRSAPVPVTTQSPPGAPPNVLWQTPLAWQNPSGWQNQPTRQTPPARQSPPTRQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPADWPLPPDWIPTDWPVPPDWQNLRPSPNLRPSPNSRASQNLGASQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	XM_005228193.3
Organism	Bos taurus(cattle)
Definition	Bos taurus MAGE family member D1 (MAGED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228193.3

ORF Insert Sequence:

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED1 ( XM_005228193.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228250.1 Bos taurus melanoma antigen family D, 1 (MAGED1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35615
Clone ID Related Accession (Same CDS sequence)	XM_005228193.3 , XM_005228193.2 , XM_015461705.1 , XM_024987905.1 , XM_024987906.1 , NM_001046125.2
Accession Version	XM_005228193.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104737.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228250.1
CDS	229..2586
Translation	MSEKMDCGAGLLGFQAEASVEDSTLLMQTLMEAIQISEAPPTSQATAAASAPNASPQSSQPPAVSEMADTQALATATKPKTAFKVQNATTKGPNAAYDFSQAFNAKETPNILPTAHFKSQNAPAKGPNAAYDFSQAAPTGELTANKSDMAFKAQNTTTKVVPNAAYNFSQSLNASEMVNPQPKTAFKAWNDTTTAPTADPQTQNVNPAKMATSHTDIEADPSPGICESDGAAAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEESSSGDQRRAPLAPGTWRSAPVPVTTQSPPGAPPNVLWQTPLAWQNPSGWQNQPTRQTPPARQSPPTRQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPADWPLPPDWIPTDWPVPPDWQNLRPSPNLRPSPNSRASQNLGASQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	XM_005228193.2
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 1 (MAGED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228193.2

ORF Insert Sequence:

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED1 ( XM_015461705.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317191.1 Bos taurus melanoma antigen family D, 1 (MAGED1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35615
Clone ID Related Accession (Same CDS sequence)	XM_005228193.3 , XM_005228193.2 , XM_015461705.1 , XM_024987905.1 , XM_024987906.1 , NM_001046125.2
Accession Version	XM_015461705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104737.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317191.1
CDS	55..2412
Translation	MSEKMDCGAGLLGFQAEASVEDSTLLMQTLMEAIQISEAPPTSQATAAASAPNASPQSSQPPAVSEMADTQALATATKPKTAFKVQNATTKGPNAAYDFSQAFNAKETPNILPTAHFKSQNAPAKGPNAAYDFSQAAPTGELTANKSDMAFKAQNTTTKVVPNAAYNFSQSLNASEMVNPQPKTAFKAWNDTTTAPTADPQTQNVNPAKMATSHTDIEADPSPGICESDGAAAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEESSSGDQRRAPLAPGTWRSAPVPVTTQSPPGAPPNVLWQTPLAWQNPSGWQNQPTRQTPPARQSPPTRQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPADWPLPPDWIPTDWPVPPDWQNLRPSPNLRPSPNSRASQNLGASQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	XM_015461705.1
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma antigen family D, 1 (MAGED1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461705.1

ORF Insert Sequence:

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED1 ( XM_024987905.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843673.1 Bos taurus MAGE family member D1 (MAGED1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35615
Clone ID Related Accession (Same CDS sequence)	XM_005228193.3 , XM_005228193.2 , XM_015461705.1 , XM_024987905.1 , XM_024987906.1 , NM_001046125.2
Accession Version	XM_024987905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843673.1
CDS	127..2484
Translation	MSEKMDCGAGLLGFQAEASVEDSTLLMQTLMEAIQISEAPPTSQATAAASAPNASPQSSQPPAVSEMADTQALATATKPKTAFKVQNATTKGPNAAYDFSQAFNAKETPNILPTAHFKSQNAPAKGPNAAYDFSQAAPTGELTANKSDMAFKAQNTTTKVVPNAAYNFSQSLNASEMVNPQPKTAFKAWNDTTTAPTADPQTQNVNPAKMATSHTDIEADPSPGICESDGAAAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEESSSGDQRRAPLAPGTWRSAPVPVTTQSPPGAPPNVLWQTPLAWQNPSGWQNQPTRQTPPARQSPPTRQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPADWPLPPDWIPTDWPVPPDWQNLRPSPNLRPSPNSRASQNLGASQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	XM_024987905.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAGE family member D1 (MAGED1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987905.1

ORF Insert Sequence:

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED1 ( XM_024987906.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843674.1 Bos taurus MAGE family member D1 (MAGED1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa35615
Clone ID Related Accession (Same CDS sequence)	XM_005228193.3 , XM_005228193.2 , XM_015461705.1 , XM_024987905.1 , XM_024987906.1 , NM_001046125.2
Accession Version	XM_024987906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	melanoma-associated antigen D1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843674.1
CDS	181..2538
Translation	MSEKMDCGAGLLGFQAEASVEDSTLLMQTLMEAIQISEAPPTSQATAAASAPNASPQSSQPPAVSEMADTQALATATKPKTAFKVQNATTKGPNAAYDFSQAFNAKETPNILPTAHFKSQNAPAKGPNAAYDFSQAAPTGELTANKSDMAFKAQNTTTKVVPNAAYNFSQSLNASEMVNPQPKTAFKAWNDTTTAPTADPQTQNVNPAKMATSHTDIEADPSPGICESDGAAAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEESSSGDQRRAPLAPGTWRSAPVPVTTQSPPGAPPNVLWQTPLAWQNPSGWQNQPTRQTPPARQSPPTRQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPADWPLPPDWIPTDWPVPPDWQNLRPSPNLRPSPNSRASQNLGASQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	XM_024987906.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAGE family member D1 (MAGED1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987906.1

ORF Insert Sequence:

ATGTCTGAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCA CCTTGCTTAT GCAGACCCTG ATGGAGGCCA TCCAGATCTC GGAGGCCCCG 
CCTACCAGCC AGGCCACAGC AGCTGCCAGT GCACCGAATG CTAGTCCTCA GAGTTCACAG 
CCCCCAGCAG TCAGTGAGAT GGCTGATACG CAGGCTTTAG CAACTGCCAC TAAGCCTAAG 
ACAGCCTTTA AGGTCCAGAA TGCCACCACA AAAGGTCCAA ATGCTGCCTA TGATTTCTCC 
CAGGCATTTA ATGCCAAGGA GACTCCCAAC ATTCTGCCTA CAGCACACTT TAAGTCCCAA 
AATGCCCCCG CAAAGGGCCC AAATGCTGCC TATGATTTTT CCCAGGCAGC CCCTACCGGT 
GAGTTAACTG CCAACAAGTC AGACATGGCC TTTAAGGCCC AGAATACCAC TACTAAAGTG 
GTCCCAAATG CGGCCTACAA TTTCTCTCAG TCTCTCAATG CCAGTGAGAT GGTCAACCCT 
CAGCCCAAGA CAGCCTTCAA GGCTTGGAAT GACACCACTA CGGCCCCTAC TGCTGATCCC 
CAGACCCAGA ATGTTAACCC AGCCAAGATG GCCACTTCCC ACACTGACAT AGAGGCTGAC 
CCAAGCCCAG GTATCTGTGA ATCTGATGGT GCAGCTGCAC AGACATCAGC GGATGGTTCC 
CAGGCTCAGA ATCTGGAGTC CAGGACTATA ATTCGGGGCA AGAGGACCCG TAAGATTAAT 
AACTTGAACG TGGAAGAGAG TAGCAGTGGG GATCAGAGGC GGGCCCCACT GGCTCCAGGG 
ACCTGGAGGT CTGCACCAGT TCCAGTTACC ACTCAGAGCC CACCTGGCGC ACCCCCCAAT 
GTGCTCTGGC AGACCCCATT GGCCTGGCAG AATCCATCAG GCTGGCAAAA TCAGCCAACC 
AGGCAGACCC CACCAGCACG TCAGAGCCCC CCAACTAGGC AGACCCCACC AGCCTGGCAA 
AACCCAGTTG CTTGGCAGAA CCCAGTGATC TGGCCAAACC CAGTGATCTG GCAGAACCCC 
GTGATCTGGC CAAACCCCAT TGTCTGGCCT GGTCCGGTTG TCTGGCCAAA CCCACTGGCC 
TGGCAGAATC CACCTGGGTG GCAGACCCCA CCTGGATGGC AGACTCCACC AGGCTGGCAA 
GGTCCTCCAG ATTGGCAAGG CCCTCCCGAT TGGCCACTAC CCCCTGACTG GCCACTGCCA 
CCCGACTGGC CGCTCCCCGC TGACTGGCCG CTCCCACCTG ACTGGATCCC CACTGATTGG 
CCAGTTCCAC CAGACTGGCA AAACCTGCGC CCCTCACCGA ACCTGCGCCC CTCTCCTAAT 
TCGCGTGCCT CACAGAACCT GGGTGCCTCA CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA 
AGAGCTAATA AGCTAGTCAA GTACCTGATG CTTAAAGATT ACACAAAGGT GCCCATCAAG 
CGCTCAGAAA TGCTAAGGGA TATCATCCGT GAATACACTG ATGTGTATCC AGAAATCATT 
GAACGTGCAT GCTTTGTCCT GGAGAAGAAA TTTGGAATTC AACTGAAAGA AATTGACAAG 
GAAGAACACC TGTATATTCT CATCAGCACC CCTGAGTCCC TGGCTGGCAT ACTGGGAACG 
ACCAAAGACA CACCAAAACT GGGTCTCCTT TTAGTGATTC TGGGTGTCAT CTTCATGAAT 
GGCAACCGTG CCAGTGAAGC TGTCCTCTGG GAGGCACTAC GCAAGATGGG ACTGCGTCCT 
GGGGTAAGAC ATCCCCTCCT TGGAGATCTG AGGAAGCTTC TCACTTACGA ATTTGTAAAA 
CAAAAGTACC TAGACTACAG ACGAGTACCC AACAGCAACC CTCCTGAGTA TGAGTTCCTC 
TGGGGCCTCC GATCCTACCA TGAGACTAGC AAGATGAAAG TGCTACGATT CATTGCAGAG 
GTTCAGAAGA GAGACCCTCG TGACTGGACT GCACAGTTCA TGGAGGCCGC AGATGAGGCC 
TTGGATGCTT TGGATGCTGC TGCAGCCGAG GCTGAGGCAC GGGCTGAGGC GAGAACCCGC 
ATGGGGATTG GAGATGAGGC CGTATCTGGG CCCTGGAGCT GGGATGACAT TGAGTTTGAG 
CTGCTGACGT GGGATGAGGA AGGAGATTTT GGAGATCCCT GGTCCAGAAT CCCATTTACC 
TTCTGGGCCA GATACCACCA GAATGCTCGC TCCAGATTTC CTCAGACCTT TGCCGGCCCC 
ATTATTGGCC CTGGTGGTAC AGCCAGTGCC AACTTTGCTG CCAACTTTGG TGCCATCGGT 
TTCTTCTGGG TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MAGED1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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