Maged1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Maged1
Entrez Gene ID	94275
Full Name	melanoma antigen, family D, 1
Synonyms	2810433C11Rik,5430405L04Rik,DXBwg1492e,Dlixin,Dlxin-1,Dlxin1,MAGE-D1,NRAGE,mFLJ00163
General protein information	Preferred Names melanoma antigen, family D, 1 Names melanoma-associated antigen D1 MAGE-D1 antigen neurotrophin receptor-interacting MAGE homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X C3\|X 41.56 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_019791.3	NP_062765.1	melanoma-associated antigen D1
NM_019791.2	NP_062765.1	melanoma-associated antigen D1

Pathways from BioSystems

Homologies

	LOC696658	XP_002808576.1
Mus musculus (house mouse)	Maged1	NP_062765.1
Rattus norvegicus (Norway rat)	Maged1	NP_445861.1
Canis lupus familiaris (dog)	MAGED1	XP_005641419.1
Bos taurus (cattle)	MAGED1	NP_001039590.2
Homo sapiens (human)	MAGED1	NP_001005333.1
Pan troglodytes (chimpanzee)	MAGED1	XP_003317513.1

Gene Ontology
Bibliography

Related articles in PubMed

Necdin modulates osteogenic cell differentiation by regulating Dlx5 and MAGE-D1.
Ju H, Lee S, Lee J, Ghil S
Biochemical and biophysical research communications489(2)109-115(2017 07)

NRAGE is involved in homologous recombination repair to resist the DNA-damaging chemotherapy and composes a ternary complex with RNF8-BARD1 to promote cell survival in squamous esophageal tumorigenesis.
Yang Q, Pan Q, Li C, Xu Y, Wen C, Sun F
Cell death and differentiation23(8)1406-16(2016 Aug)

MAGED1 is a negative regulator of bone remodeling in mice.
Liu M, Xu L, Ma X, Xu J, Wang J, Xian M, Zhou X, Wang M, Wang F, Qin A, Pan Q, Wen C
The American journal of pathology185(10)2653-67(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both transient and stable expression of Necdin induced osteoblast-specific markers in an osteogenic cell line through formation of a complex with melanoma-associated antigen D1 (MAGE-D1) and promoter activation.
Title: Necdin modulates osteogenic cell differentiation by regulating Dlx5 and MAGE-D1.

MAGED1 as a novel regulator of osteoblastogenesis, osteoclastogenesis, and bone remodeling in a mouse model
Title: MAGED1 is a negative regulator of bone remodeling in mice.

NRAGE is a potent regulator of proliferation and odontoblastic differentiation of mouse dental pulp cells, which might be via the NF-kappaB signalling pathway.
Title: Effects of neurotrophin receptor-mediated MAGE homology on proliferation and odontoblastic differentiation of mouse dental pulp cells.

Maged1 deficiency prevented the interaction of Maged1 with cAMP response element-binding protein (CREB).
Title: Maged1 co-interacting with CREB through a hexapeptide repeat domain regulates learning and memory in mice.

Maged1 is involved in osteoblast proliferation and differentiation. Mice deficient in Maged1 protein had decreased bone mineral density (BMD)
Title: Systems genetic analysis of osteoblast-lineage cells.

The following Maged1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Maged1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11455	NM_019791.3 Latest version!	Mus musculus melanoma antigen, family D, 1 (Maged1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$454.30 $649.00
View Old Accession Versions >>
OMu11455	NM_019791.2	Mus musculus melanoma antigen, family D, 1 (Maged1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$454.30 $649.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Maged1 ( NM_019791.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062765.1 Mus musculus melanoma antigen, family D, 1 (Maged1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11455
Clone ID Related Accession (Same CDS sequence)	NM_019791.3 , NM_019791.2
Accession Version	NM_019791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Mus musculus(house mouse)
Product	melanoma-associated antigen D1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627302.20. On Jul 26, 2018 this sequence version replaced NM_019791.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB029448.1, AK049660.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCAGA AACCGGACGG CGGTGCAGGC CTCCGCGGCT TCCAGGCTGA GGCCTCTGTT 
GAAGACAGCG CCTTGCTTGT GCAGACCTTG ATGGAAGCCA TCCAGATCTC CGAGGCTCCG 
CCCACCAGCC AGGCCACAGC AGCTGCCAGT GGGCCAAATG CTAGTCCCCA GAGTTCACAG 
CCCCCAACTG CCAATGAGAA GGCTGATACT GAGGTTTCAG CAGCTGCTGC CAGGCCTAAG 
ACAGGCTTTA AGGCCCAGAA TGCCACCACA AAGGGGCCAA ATGATTACTC TCAGGCACGT 
AATGCCAAGG AGATGCCCAA GAATCAGTCT AAGGCGGCCT TTAAGTCACA GAATGGCACC 
CCTAAAGGTC CACATGCTGC CTCTGACTTT TCCCAGGCAG CACCCACAGG CAAATCAGCT 
AAAAAGTCTG AAATGGCCTT TAAGGGTCAG AATAGCACTA AGGCTGGCCC CGGTACCACC 
TACAATTTCC CTCAGTCTCC CAGTGCCAAT GAGATGACCA ACAACCAGCC TAAGACAGCT 
AAGGCTTGGA ATGACACTAC TAAGGTCCCT GGAGCTGATG CCCAGACCCA GAATGTAAAT 
CAGGCCAAAA TGGCTGACGT AGGGACCAGC GCAGGTATCT CTGAAGCTGA TGGTGCAGCA 
GCCCAGACAT CAGCAGATGG CTCCCAGACT CAGAACGTGG AGTCCCGGAC TATAATTCGG 
GGCAAGAGGA CCCGCAAGGT TAATAACTTG AATGTGGAAG AGAACAACAG TGGGGATCAA 
AGGCGTGCCT CACTGGCCTC AGGGAACTGG AGGTCTGCTC CGGTTCCAGT GACCACTCAG 
CAGAACCCAC CTGGAGCACC CCCTAATGTG GTGTGGCAGA CACCACTGGC TTGGCAGAAC 
CCATCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCAGCACG TCAGAGTCCC 
CCAGCTAGGC AGACACCATC AGCTTGGCAG AACCCAGTTG CATGGCAGAA TCCAGTGATC 
TGGCCTAACC CAGTGATCTG GCAGAATCCA GTGATCTGGC CAAACCCCAT TGTCTGGCCT 
GGCCCAATTG TCTGGCCAAA CCCAATGGCC TGGCAGAGTA CACCTGGATG GCAGAGCCCA 
CCCAGCTGGC AGGCTCCACC TAGTTGGCAG AGCCCTCAAG ATTGGCAGGG CCCTCCAGAT 
TGGCAGGTAC CACCTGACTG GTCAATGCCT CCTGACTGGT CCTTTCCCTC CGACTGGCCT 
TTTCCACCTG ACTGGATCCC CGCCGACTGG CCAATTCCAC CTGACTGGCA GAACTTACGA 
CCCTCACCTA ATCTGAGATC CTCCTCCAAC TCTCGTGCCT CACAGAACCA GGGTCCTCCA 
CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA AGAGCAAATA AGTTGGTCAA GTACCTGATG 
CTTAAAGACT ACACGAAGGT GCCCATCAAG CGCTCAGAAA TGCTGAGGGA TATCATCCGA 
GAATACACTG ATGTCTATCC AGAAATCATT GAGCGCGCAT GCTTTGTCCT GGAGAAGAAA 
TTTGGAATCC AGCTGAAGGA AATCGACAAA GAAGAGCATC TGTATATTCT CATCAGTACC 
CCTGAGTCCC TGGCTGGCAT ACTGGGAACG ACCAAAGACA CACCGAAGCT TGGCCTCCTC 
TTAGTGATTC TGGGCATTAT CTTCATGAAT GGCAACCGTG CCACCGAGGC CGTCCTCTGG 
GAAGCACTGC GCAAGATGGG ACTACGTCCT GGGGTGAGAC ATCCCCTCCT TGGTGACCTG 
AGAAAACTTC TTACTTACGA GTTTGTAAAG CAGAAATACC TGGACTATAG ACGAGTGCCC 
AACAGCAACC CTCCTGAGTA TGAGTTCCTC TGGGGCCTCC GCTCCTACCA TGAGACTAGC 
AAGATGAAAG TGCTGAGATT CATTGCAGAG GTTCAGAAGA GAGACCCTCG TGACTGGACT 
GCACAGTTCA TGGAAGCTGC AGATGAAGCC TTGGATGCTC TGGATGCTGC TGCAGCTGAG 
GCAGAGGCCC GGGCTGAAGC GAGAAACCGC ATGGGAATTG GAGATGAGGC TGTGTCTGGT 
CCCTGGAGCT GGGATGACAT TGAGTTTGAG CTGCTGACCT GGGATGAGGA AGGAGATTTT 
GGAGATCCTT GGTCCAGGAT CCCCTTTACC TTCTGGGCCA GATACCACCA GAATGCCCGC 
TCCAGGTTTC CCCAGGCCTT TACTGGCCCC ATCATTGGCC CCAGTGGTAC TGCCACCGCC 
AACTTCGCCG CCAACTTCGG TGCCATTGGC TTCTTCTGGG TTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062765.1
CDS	164..2491
Translation	MAQKPDGGAGLRGFQAEASVEDSALLVQTLMEAIQISEAPPTSQATAAASGPNASPQSSQPPTANEKADTEVSAAAARPKTGFKAQNATTKGPNDYSQARNAKEMPKNQSKAAFKSQNGTPKGPHAASDFSQAAPTGKSAKKSEMAFKGQNSTKAGPGTTYNFPQSPSANEMTNNQPKTAKAWNDTTKVPGADAQTQNVNQAKMADVGTSAGISEADGAAAQTSADGSQTQNVESRTIIRGKRTRKVNNLNVEENNSGDQRRASLASGNWRSAPVPVTTQQNPPGAPPNVVWQTPLAWQNPSGWQNQTARQTPPAARQSPPARQTPSAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPIVWPNPMAWQSTPGWQSPPSWQAPPSWQSPQDWQGPPDWQVPPDWSMPPDWSFPSDWPFPPDWIPADWPIPPDWQNLRPSPNLRSSSNSRASQNQGPPQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGIIFMNGNRATEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARNRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQAFTGPIIGPSGTATANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	NM_019791.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanoma antigen, family D, 1 (Maged1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019791.3

ORF Insert Sequence:

ATGGCTCAGA AACCGGACGG CGGTGCAGGC CTCCGCGGCT TCCAGGCTGA GGCCTCTGTT 
GAAGACAGCG CCTTGCTTGT GCAGACCTTG ATGGAAGCCA TCCAGATCTC CGAGGCTCCG 
CCCACCAGCC AGGCCACAGC AGCTGCCAGT GGGCCAAATG CTAGTCCCCA GAGTTCACAG 
CCCCCAACTG CCAATGAGAA GGCTGATACT GAGGTTTCAG CAGCTGCTGC CAGGCCTAAG 
ACAGGCTTTA AGGCCCAGAA TGCCACCACA AAGGGGCCAA ATGATTACTC TCAGGCACGT 
AATGCCAAGG AGATGCCCAA GAATCAGTCT AAGGCGGCCT TTAAGTCACA GAATGGCACC 
CCTAAAGGTC CACATGCTGC CTCTGACTTT TCCCAGGCAG CACCCACAGG CAAATCAGCT 
AAAAAGTCTG AAATGGCCTT TAAGGGTCAG AATAGCACTA AGGCTGGCCC CGGTACCACC 
TACAATTTCC CTCAGTCTCC CAGTGCCAAT GAGATGACCA ACAACCAGCC TAAGACAGCT 
AAGGCTTGGA ATGACACTAC TAAGGTCCCT GGAGCTGATG CCCAGACCCA GAATGTAAAT 
CAGGCCAAAA TGGCTGACGT AGGGACCAGC GCAGGTATCT CTGAAGCTGA TGGTGCAGCA 
GCCCAGACAT CAGCAGATGG CTCCCAGACT CAGAACGTGG AGTCCCGGAC TATAATTCGG 
GGCAAGAGGA CCCGCAAGGT TAATAACTTG AATGTGGAAG AGAACAACAG TGGGGATCAA 
AGGCGTGCCT CACTGGCCTC AGGGAACTGG AGGTCTGCTC CGGTTCCAGT GACCACTCAG 
CAGAACCCAC CTGGAGCACC CCCTAATGTG GTGTGGCAGA CACCACTGGC TTGGCAGAAC 
CCATCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCAGCACG TCAGAGTCCC 
CCAGCTAGGC AGACACCATC AGCTTGGCAG AACCCAGTTG CATGGCAGAA TCCAGTGATC 
TGGCCTAACC CAGTGATCTG GCAGAATCCA GTGATCTGGC CAAACCCCAT TGTCTGGCCT 
GGCCCAATTG TCTGGCCAAA CCCAATGGCC TGGCAGAGTA CACCTGGATG GCAGAGCCCA 
CCCAGCTGGC AGGCTCCACC TAGTTGGCAG AGCCCTCAAG ATTGGCAGGG CCCTCCAGAT 
TGGCAGGTAC CACCTGACTG GTCAATGCCT CCTGACTGGT CCTTTCCCTC CGACTGGCCT 
TTTCCACCTG ACTGGATCCC CGCCGACTGG CCAATTCCAC CTGACTGGCA GAACTTACGA 
CCCTCACCTA ATCTGAGATC CTCCTCCAAC TCTCGTGCCT CACAGAACCA GGGTCCTCCA 
CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA AGAGCAAATA AGTTGGTCAA GTACCTGATG 
CTTAAAGACT ACACGAAGGT GCCCATCAAG CGCTCAGAAA TGCTGAGGGA TATCATCCGA 
GAATACACTG ATGTCTATCC AGAAATCATT GAGCGCGCAT GCTTTGTCCT GGAGAAGAAA 
TTTGGAATCC AGCTGAAGGA AATCGACAAA GAAGAGCATC TGTATATTCT CATCAGTACC 
CCTGAGTCCC TGGCTGGCAT ACTGGGAACG ACCAAAGACA CACCGAAGCT TGGCCTCCTC 
TTAGTGATTC TGGGCATTAT CTTCATGAAT GGCAACCGTG CCACCGAGGC CGTCCTCTGG 
GAAGCACTGC GCAAGATGGG ACTACGTCCT GGGGTGAGAC ATCCCCTCCT TGGTGACCTG 
AGAAAACTTC TTACTTACGA GTTTGTAAAG CAGAAATACC TGGACTATAG ACGAGTGCCC 
AACAGCAACC CTCCTGAGTA TGAGTTCCTC TGGGGCCTCC GCTCCTACCA TGAGACTAGC 
AAGATGAAAG TGCTGAGATT CATTGCAGAG GTTCAGAAGA GAGACCCTCG TGACTGGACT 
GCACAGTTCA TGGAAGCTGC AGATGAAGCC TTGGATGCTC TGGATGCTGC TGCAGCTGAG 
GCAGAGGCCC GGGCTGAAGC GAGAAACCGC ATGGGAATTG GAGATGAGGC TGTGTCTGGT 
CCCTGGAGCT GGGATGACAT TGAGTTTGAG CTGCTGACCT GGGATGAGGA AGGAGATTTT 
GGAGATCCTT GGTCCAGGAT CCCCTTTACC TTCTGGGCCA GATACCACCA GAATGCCCGC 
TCCAGGTTTC CCCAGGCCTT TACTGGCCCC ATCATTGGCC CCAGTGGTAC TGCCACCGCC 
AACTTCGCCG CCAACTTCGG TGCCATTGGC TTCTTCTGGG TTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Maged1 ( NM_019791.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062765.1 Mus musculus melanoma antigen, family D, 1 (Maged1), mRNA.

Price & Availability↑

CloneID	OMu11455
Clone ID Related Accession (Same CDS sequence)	NM_019791.3 , NM_019791.2
Accession Version	NM_019791.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Mus musculus(house mouse)
Product	melanoma-associated antigen D1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL627302.20. On Nov 17, 2006 this sequence version replaced NM_019791.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB029448.1, SRR1660817.230387.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGA AACCGGACGG CGGTGCAGGC CTCCGCGGCT TCCAGGCTGA GGCCTCTGTT 
GAAGACAGCG CCTTGCTTGT GCAGACCTTG ATGGAAGCCA TCCAGATCTC CGAGGCTCCG 
CCCACCAGCC AGGCCACAGC AGCTGCCAGT GGGCCAAATG CTAGTCCCCA GAGTTCACAG 
CCCCCAACTG CCAATGAGAA GGCTGATACT GAGGTTTCAG CAGCTGCTGC CAGGCCTAAG 
ACAGGCTTTA AGGCCCAGAA TGCCACCACA AAGGGGCCAA ATGATTACTC TCAGGCACGT 
AATGCCAAGG AGATGCCCAA GAATCAGTCT AAGGCGGCCT TTAAGTCACA GAATGGCACC 
CCTAAAGGTC CACATGCTGC CTCTGACTTT TCCCAGGCAG CACCCACAGG CAAATCAGCT 
AAAAAGTCTG AAATGGCCTT TAAGGGTCAG AATAGCACTA AGGCTGGCCC CGGTACCACC 
TACAATTTCC CTCAGTCTCC CAGTGCCAAT GAGATGACCA ACAACCAGCC TAAGACAGCT 
AAGGCTTGGA ATGACACTAC TAAGGTCCCT GGAGCTGATG CCCAGACCCA GAATGTAAAT 
CAGGCCAAAA TGGCTGACGT AGGGACCAGC GCAGGTATCT CTGAAGCTGA TGGTGCAGCA 
GCCCAGACAT CAGCAGATGG CTCCCAGACT CAGAACGTGG AGTCCCGGAC TATAATTCGG 
GGCAAGAGGA CCCGCAAGGT TAATAACTTG AATGTGGAAG AGAACAACAG TGGGGATCAA 
AGGCGTGCCT CACTGGCCTC AGGGAACTGG AGGTCTGCTC CGGTTCCAGT GACCACTCAG 
CAGAACCCAC CTGGAGCACC CCCTAATGTG GTGTGGCAGA CACCACTGGC TTGGCAGAAC 
CCATCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCAGCACG TCAGAGTCCC 
CCAGCTAGGC AGACACCATC AGCTTGGCAG AACCCAGTTG CATGGCAGAA TCCAGTGATC 
TGGCCTAACC CAGTGATCTG GCAGAATCCA GTGATCTGGC CAAACCCCAT TGTCTGGCCT 
GGCCCAATTG TCTGGCCAAA CCCAATGGCC TGGCAGAGTA CACCTGGATG GCAGAGCCCA 
CCCAGCTGGC AGGCTCCACC TAGTTGGCAG AGCCCTCAAG ATTGGCAGGG CCCTCCAGAT 
TGGCAGGTAC CACCTGACTG GTCAATGCCT CCTGACTGGT CCTTTCCCTC CGACTGGCCT 
TTTCCACCTG ACTGGATCCC CGCCGACTGG CCAATTCCAC CTGACTGGCA GAACTTACGA 
CCCTCACCTA ATCTGAGATC CTCCTCCAAC TCTCGTGCCT CACAGAACCA GGGTCCTCCA 
CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA AGAGCAAATA AGTTGGTCAA GTACCTGATG 
CTTAAAGACT ACACGAAGGT GCCCATCAAG CGCTCAGAAA TGCTGAGGGA TATCATCCGA 
GAATACACTG ATGTCTATCC AGAAATCATT GAGCGCGCAT GCTTTGTCCT GGAGAAGAAA 
TTTGGAATCC AGCTGAAGGA AATCGACAAA GAAGAGCATC TGTATATTCT CATCAGTACC 
CCTGAGTCCC TGGCTGGCAT ACTGGGAACG ACCAAAGACA CACCGAAGCT TGGCCTCCTC 
TTAGTGATTC TGGGCATTAT CTTCATGAAT GGCAACCGTG CCACCGAGGC CGTCCTCTGG 
GAAGCACTGC GCAAGATGGG ACTACGTCCT GGGGTGAGAC ATCCCCTCCT TGGTGACCTG 
AGAAAACTTC TTACTTACGA GTTTGTAAAG CAGAAATACC TGGACTATAG ACGAGTGCCC 
AACAGCAACC CTCCTGAGTA TGAGTTCCTC TGGGGCCTCC GCTCCTACCA TGAGACTAGC 
AAGATGAAAG TGCTGAGATT CATTGCAGAG GTTCAGAAGA GAGACCCTCG TGACTGGACT 
GCACAGTTCA TGGAAGCTGC AGATGAAGCC TTGGATGCTC TGGATGCTGC TGCAGCTGAG 
GCAGAGGCCC GGGCTGAAGC GAGAAACCGC ATGGGAATTG GAGATGAGGC TGTGTCTGGT 
CCCTGGAGCT GGGATGACAT TGAGTTTGAG CTGCTGACCT GGGATGAGGA AGGAGATTTT 
GGAGATCCTT GGTCCAGGAT CCCCTTTACC TTCTGGGCCA GATACCACCA GAATGCCCGC 
TCCAGGTTTC CCCAGGCCTT TACTGGCCCC ATCATTGGCC CCAGTGGTAC TGCCACCGCC 
AACTTCGCCG CCAACTTCGG TGCCATTGGC TTCTTCTGGG TTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062765.1
CDS	213..2540
Translation	MAQKPDGGAGLRGFQAEASVEDSALLVQTLMEAIQISEAPPTSQATAAASGPNASPQSSQPPTANEKADTEVSAAAARPKTGFKAQNATTKGPNDYSQARNAKEMPKNQSKAAFKSQNGTPKGPHAASDFSQAAPTGKSAKKSEMAFKGQNSTKAGPGTTYNFPQSPSANEMTNNQPKTAKAWNDTTKVPGADAQTQNVNQAKMADVGTSAGISEADGAAAQTSADGSQTQNVESRTIIRGKRTRKVNNLNVEENNSGDQRRASLASGNWRSAPVPVTTQQNPPGAPPNVVWQTPLAWQNPSGWQNQTARQTPPAARQSPPARQTPSAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPIVWPNPMAWQSTPGWQSPPSWQAPPSWQSPQDWQGPPDWQVPPDWSMPPDWSFPSDWPFPPDWIPADWPIPPDWQNLRPSPNLRSSSNSRASQNQGPPQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGIIFMNGNRATEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARNRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQAFTGPIIGPSGTATANFAANFGAIGFFWVE

Target ORF information:

RefSeq Version	NM_019791.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanoma antigen, family D, 1 (Maged1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019791.2

ORF Insert Sequence:

ATGGCTCAGA AACCGGACGG CGGTGCAGGC CTCCGCGGCT TCCAGGCTGA GGCCTCTGTT 
GAAGACAGCG CCTTGCTTGT GCAGACCTTG ATGGAAGCCA TCCAGATCTC CGAGGCTCCG 
CCCACCAGCC AGGCCACAGC AGCTGCCAGT GGGCCAAATG CTAGTCCCCA GAGTTCACAG 
CCCCCAACTG CCAATGAGAA GGCTGATACT GAGGTTTCAG CAGCTGCTGC CAGGCCTAAG 
ACAGGCTTTA AGGCCCAGAA TGCCACCACA AAGGGGCCAA ATGATTACTC TCAGGCACGT 
AATGCCAAGG AGATGCCCAA GAATCAGTCT AAGGCGGCCT TTAAGTCACA GAATGGCACC 
CCTAAAGGTC CACATGCTGC CTCTGACTTT TCCCAGGCAG CACCCACAGG CAAATCAGCT 
AAAAAGTCTG AAATGGCCTT TAAGGGTCAG AATAGCACTA AGGCTGGCCC CGGTACCACC 
TACAATTTCC CTCAGTCTCC CAGTGCCAAT GAGATGACCA ACAACCAGCC TAAGACAGCT 
AAGGCTTGGA ATGACACTAC TAAGGTCCCT GGAGCTGATG CCCAGACCCA GAATGTAAAT 
CAGGCCAAAA TGGCTGACGT AGGGACCAGC GCAGGTATCT CTGAAGCTGA TGGTGCAGCA 
GCCCAGACAT CAGCAGATGG CTCCCAGACT CAGAACGTGG AGTCCCGGAC TATAATTCGG 
GGCAAGAGGA CCCGCAAGGT TAATAACTTG AATGTGGAAG AGAACAACAG TGGGGATCAA 
AGGCGTGCCT CACTGGCCTC AGGGAACTGG AGGTCTGCTC CGGTTCCAGT GACCACTCAG 
CAGAACCCAC CTGGAGCACC CCCTAATGTG GTGTGGCAGA CACCACTGGC TTGGCAGAAC 
CCATCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCAGCACG TCAGAGTCCC 
CCAGCTAGGC AGACACCATC AGCTTGGCAG AACCCAGTTG CATGGCAGAA TCCAGTGATC 
TGGCCTAACC CAGTGATCTG GCAGAATCCA GTGATCTGGC CAAACCCCAT TGTCTGGCCT 
GGCCCAATTG TCTGGCCAAA CCCAATGGCC TGGCAGAGTA CACCTGGATG GCAGAGCCCA 
CCCAGCTGGC AGGCTCCACC TAGTTGGCAG AGCCCTCAAG ATTGGCAGGG CCCTCCAGAT 
TGGCAGGTAC CACCTGACTG GTCAATGCCT CCTGACTGGT CCTTTCCCTC CGACTGGCCT 
TTTCCACCTG ACTGGATCCC CGCCGACTGG CCAATTCCAC CTGACTGGCA GAACTTACGA 
CCCTCACCTA ATCTGAGATC CTCCTCCAAC TCTCGTGCCT CACAGAACCA GGGTCCTCCA 
CAGCCCCGAG ATGTGGCCCT TCTTCAGGAA AGAGCAAATA AGTTGGTCAA GTACCTGATG 
CTTAAAGACT ACACGAAGGT GCCCATCAAG CGCTCAGAAA TGCTGAGGGA TATCATCCGA 
GAATACACTG ATGTCTATCC AGAAATCATT GAGCGCGCAT GCTTTGTCCT GGAGAAGAAA 
TTTGGAATCC AGCTGAAGGA AATCGACAAA GAAGAGCATC TGTATATTCT CATCAGTACC 
CCTGAGTCCC TGGCTGGCAT ACTGGGAACG ACCAAAGACA CACCGAAGCT TGGCCTCCTC 
TTAGTGATTC TGGGCATTAT CTTCATGAAT GGCAACCGTG CCACCGAGGC CGTCCTCTGG 
GAAGCACTGC GCAAGATGGG ACTACGTCCT GGGGTGAGAC ATCCCCTCCT TGGTGACCTG 
AGAAAACTTC TTACTTACGA GTTTGTAAAG CAGAAATACC TGGACTATAG ACGAGTGCCC 
AACAGCAACC CTCCTGAGTA TGAGTTCCTC TGGGGCCTCC GCTCCTACCA TGAGACTAGC 
AAGATGAAAG TGCTGAGATT CATTGCAGAG GTTCAGAAGA GAGACCCTCG TGACTGGACT 
GCACAGTTCA TGGAAGCTGC AGATGAAGCC TTGGATGCTC TGGATGCTGC TGCAGCTGAG 
GCAGAGGCCC GGGCTGAAGC GAGAAACCGC ATGGGAATTG GAGATGAGGC TGTGTCTGGT 
CCCTGGAGCT GGGATGACAT TGAGTTTGAG CTGCTGACCT GGGATGAGGA AGGAGATTTT 
GGAGATCCTT GGTCCAGGAT CCCCTTTACC TTCTGGGCCA GATACCACCA GAATGCCCGC 
TCCAGGTTTC CCCAGGCCTT TACTGGCCCC ATCATTGGCC CCAGTGGTAC TGCCACCGCC 
AACTTCGCCG CCAACTTCGG TGCCATTGGC TTCTTCTGGG TTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Necdin modulates osteogenic cell differentiation by regulating Dlx5 and MAGE-D1.
Biochemical and biophysical research communications489(2)109-115(2017 07)
Ju H,Lee S,Lee J,Ghil S

NRAGE is involved in homologous recombination repair to resist the DNA-damaging chemotherapy and composes a ternary complex with RNF8-BARD1 to promote cell survival in squamous esophageal tumorigenesis.
Cell death and differentiation23(8)1406-16(2016 Aug)
Yang Q,Pan Q,Li C,Xu Y,Wen C,Sun F

MAGED1 is a negative regulator of bone remodeling in mice.
The American journal of pathology185(10)2653-67(2015 Oct)
Liu M,Xu L,Ma X,Xu J,Wang J,Xian M,Zhou X,Wang M,Wang F,Qin A,Pan Q,Wen C

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Maged1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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