MAGED1 cDNA ORF clone, Homo sapiens(human)

The following MAGED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03665 NM_001005333.2
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu18181 NM_006986.4
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu18181 NM_001005332.2
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu18181 XM_017029978.1
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu18181 XM_011530835.2
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu18181 XM_017029980.1
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu18181 XM_017029979.1
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
View Old Accession Versions >>
OHu03665 NM_001005333.1 Homo sapiens MAGE family member D1 (MAGED1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu18181 NM_006986.3 Homo sapiens MAGE family member D1 (MAGED1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu18181 NM_001005332.1 Homo sapiens MAGE family member D1 (MAGED1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu18181 XM_011530835.1 Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu03665
    Clone ID Related Accession (Same CDS sequence) NM_001005333.1 , NM_001005333.2
    Accession Version NM_001005333.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-12
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX422007.2, BC014070.2 and AF132205.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014070.2, SRR1660809.249174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGAATCC TGACGCTTGT 
    AGAGCAGTCT GTCACCCCCT CCCCCAGCCT CCTGCCTCCA CCCTCCCTCT TTCTGCATTC
    CCCACTCTGT GCGACCCCCC TTATTCTCAA CTCCGCGATC CGCCTGCCGT CCTGAGCTGC
    TACTGCACGC CTCTTGGAGC GTCCCCGGCT CCTGCTGAGG CCTCCGTAGA AGACAGCGCC
    TTGCTTATGC AGACCTTGAT GGAGGCCATC CAGATCTCAG AGGCTCCACC TACTAACCAG
    GCCACCGCAG CTGCTAGTCC CCAGAGTTCA CAGCCCCCAA CTGCCAATGA GATGGCTGAC
    ATTCAGGTTT CAGCAGCTGC CGCTAGGCCT AAGTCAGCCT TTAAAGTCCA GAATGCCACC
    ACAAAAGGCC CAAATGGTGT CTATGATTTC TCTCAGGCTC ATAATGCCAA GGATGTGCCC
    AACACGCAGC CCAAGGCAGC CTTTAAGTCC CAAAATGCTA CCCCAAAGGG TCCAAATGCT
    GCCTATGATT TTTCCCAGGC AGCAACCACT GGTGAGTTAG CTGCTAACAA GTCTGAGATG
    GCCTTCAAGG CCCAGAATGC CACTACTAAA GTGGGCCCAA ATGCCACCTA CAATTTCTCT
    CAGTCTCTCA ATGCCAATGA CCTGGCCAAC AGCAGGCCTA AGACCCCTTT CAAGGCTTGG
    AATGATACCA CTAAGGCCCC AACAGCTGAT ACCCAGACCC AGAATGTAAA TCAGGCCAAA
    ATGGCCACTT CCCAGGCTGA CATAGAGACC GACCCAGGTA TCTCTGAACC TGACGGTGCA
    ACTGCACAGA CATCAGCAGA TGGTTCCCAG GCTCAGAATC TGGAGTCCCG GACAATAATT
    CGGGGCAAGA GGACCCGCAA GATTAATAAC TTGAATGTTG AAGAGAACAG CAGTGGGGAT
    CAGAGGCGGG CCCCACTGGC TGCAGGGACC TGGAGGTCTG CACCAGTTCC AGTGACCACT
    CAGAACCCAC CTGGCGCACC CCCCAATGTG CTCTGGCAGA CGCCATTGGC TTGGCAGAAC
    CCCTCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCACGTCA GAGCCCTCCA
    GCTAGGCAGA CCCCACCAGC CTGGCAGAAC CCAGTCGCTT GGCAGAACCC AGTGATTTGG
    CCAAACCCAG TAATCTGGCA GAACCCAGTG ATCTGGCCAA ACCCCATTGT CTGGCCCGGC
    CCTGTTGTCT GGCCGAATCC ACTGGCCTGG CAGAATCCAC CTGGATGGCA GACTCCACCT
    GGATGGCAGA CCCCACCGGG CTGGCAGGGT CCTCCAGACT GGCAAGGTCC TCCTGACTGG
    CCGCTACCAC CCGACTGGCC ACTGCCACCT GATTGGCCAC TTCCCACTGA CTGGCCACTA
    CCACCTGACT GGATCCCCGC TGATTGGCCA ATTCCACCTG ACTGGCAGAA CCTGCGCCCC
    TCGCCTAACC TGCGCCCTTC TCCCAACTCG CGTGCCTCAC AGAACCCAGG TGCTGCACAG
    CCCCGAGATG TGGCCCTTCT TCAGGAAAGA GCAAATAAGT TGGTCAAGTA CTTGATGCTT
    AAGGACTACA CAAAGGTGCC CATCAAGCGC TCAGAAATGC TGAGAGATAT CATCCGTGAA
    TACACTGATG TTTATCCAGA AATCATTGAA CGTGCATGCT TTGTCCTAGA GAAGAAATTT
    GGGATTCAAC TGAAAGAAAT TGACAAAGAA GAACACCTGT ATATTCTCAT CAGTACCCCC
    GAGTCCCTGG CTGGCATACT GGGAACGACC AAAGACACAC CCAAGCTCGG TCTCCTCTTG
    GTGATTCTGG GTGTCATCTT CATGAATGGC AACCGTGCCA GTGAGGCTGT CCTCTGGGAG
    GCACTACGCA AGATGGGACT GCGTCCTGGG GTGAGACATC CCCTCCTTGG AGATCTAAGG
    AAACTTCTCA CCTATGAGTT TGTAAAGCAG AAATACCTGG ACTACAGACG AGTGCCCAAC
    AGCAACCCCC CGGAGTATGA GTTCCTCTGG GGCCTCCGTT CCTACCATGA GACTAGCAAG
    ATGAAAGTGC TGAGATTCAT TGCAGAGGTT CAGAAAAGAG ACCCTCGTGA CTGGACTGCA
    CAGTTCATGG AGGCTGCAGA TGAGGCCTTG GATGCTCTGG ATGCTGCTGC AGCTGAGGCC
    GAAGCCCGGG CTGAAGCAAG AACCCGCATG GGAATTGGAG ATGAGGCTGT GTCTGGGCCC
    TGGAGCTGGG ATGACATTGA GTTTGAGCTG CTGACCTGGG ATGAGGAAGG AGATTTTGGA
    GATCCCTGGT CCAGAATTCC ATTTACCTTC TGGGCCAGAT ACCACCAGAA TGCCCGCTCC
    AGATTCCCTC AGACCTTTGC CGGTCCCATT ATTGGTCCTG GTGGTACAGC CAGTGCCAAC
    TTCGCTGCCA ACTTTGGTGC CATTGGTTTC TTCTGGGTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001005333.1
    CDS191..2695
    Translation

    Target ORF information:

    RefSeq Version NM_001005333.1
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001005333.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGAATCC TGACGCTTGT 
    AGAGCAGTCT GTCACCCCCT CCCCCAGCCT CCTGCCTCCA CCCTCCCTCT TTCTGCATTC
    CCCACTCTGT GCGACCCCCC TTATTCTCAA CTCCGCGATC CGCCTGCCGT CCTGAGCTGC
    TACTGCACGC CTCTTGGAGC GTCCCCGGCT CCTGCTGAGG CCTCCGTAGA AGACAGCGCC
    TTGCTTATGC AGACCTTGAT GGAGGCCATC CAGATCTCAG AGGCTCCACC TACTAACCAG
    GCCACCGCAG CTGCTAGTCC CCAGAGTTCA CAGCCCCCAA CTGCCAATGA GATGGCTGAC
    ATTCAGGTTT CAGCAGCTGC CGCTAGGCCT AAGTCAGCCT TTAAAGTCCA GAATGCCACC
    ACAAAAGGCC CAAATGGTGT CTATGATTTC TCTCAGGCTC ATAATGCCAA GGATGTGCCC
    AACACGCAGC CCAAGGCAGC CTTTAAGTCC CAAAATGCTA CCCCAAAGGG TCCAAATGCT
    GCCTATGATT TTTCCCAGGC AGCAACCACT GGTGAGTTAG CTGCTAACAA GTCTGAGATG
    GCCTTCAAGG CCCAGAATGC CACTACTAAA GTGGGCCCAA ATGCCACCTA CAATTTCTCT
    CAGTCTCTCA ATGCCAATGA CCTGGCCAAC AGCAGGCCTA AGACCCCTTT CAAGGCTTGG
    AATGATACCA CTAAGGCCCC AACAGCTGAT ACCCAGACCC AGAATGTAAA TCAGGCCAAA
    ATGGCCACTT CCCAGGCTGA CATAGAGACC GACCCAGGTA TCTCTGAACC TGACGGTGCA
    ACTGCACAGA CATCAGCAGA TGGTTCCCAG GCTCAGAATC TGGAGTCCCG GACAATAATT
    CGGGGCAAGA GGACCCGCAA GATTAATAAC TTGAATGTTG AAGAGAACAG CAGTGGGGAT
    CAGAGGCGGG CCCCACTGGC TGCAGGGACC TGGAGGTCTG CACCAGTTCC AGTGACCACT
    CAGAACCCAC CTGGCGCACC CCCCAATGTG CTCTGGCAGA CGCCATTGGC TTGGCAGAAC
    CCCTCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCACGTCA GAGCCCTCCA
    GCTAGGCAGA CCCCACCAGC CTGGCAGAAC CCAGTCGCTT GGCAGAACCC AGTGATTTGG
    CCAAACCCAG TAATCTGGCA GAACCCAGTG ATCTGGCCAA ACCCCATTGT CTGGCCCGGC
    CCTGTTGTCT GGCCGAATCC ACTGGCCTGG CAGAATCCAC CTGGATGGCA GACTCCACCT
    GGATGGCAGA CCCCACCGGG CTGGCAGGGT CCTCCAGACT GGCAAGGTCC TCCTGACTGG
    CCGCTACCAC CCGACTGGCC ACTGCCACCT GATTGGCCAC TTCCCACTGA CTGGCCACTA
    CCACCTGACT GGATCCCCGC TGATTGGCCA ATTCCACCTG ACTGGCAGAA CCTGCGCCCC
    TCGCCTAACC TGCGCCCTTC TCCCAACTCG CGTGCCTCAC AGAACCCAGG TGCTGCACAG
    CCCCGAGATG TGGCCCTTCT TCAGGAAAGA GCAAATAAGT TGGTCAAGTA CTTGATGCTT
    AAGGACTACA CAAAGGTGCC CATCAAGCGC TCAGAAATGC TGAGAGATAT CATCCGTGAA
    TACACTGATG TTTATCCAGA AATCATTGAA CGTGCATGCT TTGTCCTAGA GAAGAAATTT
    GGGATTCAAC TGAAAGAAAT TGACAAAGAA GAACACCTGT ATATTCTCAT CAGTACCCCC
    GAGTCCCTGG CTGGCATACT GGGAACGACC AAAGACACAC CCAAGCTCGG TCTCCTCTTG
    GTGATTCTGG GTGTCATCTT CATGAATGGC AACCGTGCCA GTGAGGCTGT CCTCTGGGAG
    GCACTACGCA AGATGGGACT GCGTCCTGGG GTGAGACATC CCCTCCTTGG AGATCTAAGG
    AAACTTCTCA CCTATGAGTT TGTAAAGCAG AAATACCTGG ACTACAGACG AGTGCCCAAC
    AGCAACCCCC CGGAGTATGA GTTCCTCTGG GGCCTCCGTT CCTACCATGA GACTAGCAAG
    ATGAAAGTGC TGAGATTCAT TGCAGAGGTT CAGAAAAGAG ACCCTCGTGA CTGGACTGCA
    CAGTTCATGG AGGCTGCAGA TGAGGCCTTG GATGCTCTGG ATGCTGCTGC AGCTGAGGCC
    GAAGCCCGGG CTGAAGCAAG AACCCGCATG GGAATTGGAG ATGAGGCTGT GTCTGGGCCC
    TGGAGCTGGG ATGACATTGA GTTTGAGCTG CTGACCTGGG ATGAGGAAGG AGATTTTGGA
    GATCCCTGGT CCAGAATTCC ATTTACCTTC TGGGCCAGAT ACCACCAGAA TGCCCGCTCC
    AGATTCCCTC AGACCTTTGC CGGTCCCATT ATTGGTCCTG GTGGTACAGC CAGTGCCAAC
    TTCGCTGCCA ACTTTGGTGC CATTGGTTTC TTCTGGGTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03665
    Clone ID Related Accession (Same CDS sequence) NM_001005333.1 , NM_001005333.2
    Accession Version NM_001005333.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX422007.2 and BC014070.2. On Jun 2, 2019 this sequence version replaced NM_001005333.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014070.2, SRR1660809.249174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGAATCC TGACGCTTGT 
    AGAGCAGTCT GTCACCCCCT CCCCCAGCCT CCTGCCTCCA CCCTCCCTCT TTCTGCATTC
    CCCACTCTGT GCGACCCCCC TTATTCTCAA CTCCGCGATC CGCCTGCCGT CCTGAGCTGC
    TACTGCACGC CTCTTGGAGC GTCCCCGGCT CCTGCTGAGG CCTCCGTAGA AGACAGCGCC
    TTGCTTATGC AGACCTTGAT GGAGGCCATC CAGATCTCAG AGGCTCCACC TACTAACCAG
    GCCACCGCAG CTGCTAGTCC CCAGAGTTCA CAGCCCCCAA CTGCCAATGA GATGGCTGAC
    ATTCAGGTTT CAGCAGCTGC CGCTAGGCCT AAGTCAGCCT TTAAAGTCCA GAATGCCACC
    ACAAAAGGCC CAAATGGTGT CTATGATTTC TCTCAGGCTC ATAATGCCAA GGATGTGCCC
    AACACGCAGC CCAAGGCAGC CTTTAAGTCC CAAAATGCTA CCCCAAAGGG TCCAAATGCT
    GCCTATGATT TTTCCCAGGC AGCAACCACT GGTGAGTTAG CTGCTAACAA GTCTGAGATG
    GCCTTCAAGG CCCAGAATGC CACTACTAAA GTGGGCCCAA ATGCCACCTA CAATTTCTCT
    CAGTCTCTCA ATGCCAATGA CCTGGCCAAC AGCAGGCCTA AGACCCCTTT CAAGGCTTGG
    AATGATACCA CTAAGGCCCC AACAGCTGAT ACCCAGACCC AGAATGTAAA TCAGGCCAAA
    ATGGCCACTT CCCAGGCTGA CATAGAGACC GACCCAGGTA TCTCTGAACC TGACGGTGCA
    ACTGCACAGA CATCAGCAGA TGGTTCCCAG GCTCAGAATC TGGAGTCCCG GACAATAATT
    CGGGGCAAGA GGACCCGCAA GATTAATAAC TTGAATGTTG AAGAGAACAG CAGTGGGGAT
    CAGAGGCGGG CCCCACTGGC TGCAGGGACC TGGAGGTCTG CACCAGTTCC AGTGACCACT
    CAGAACCCAC CTGGCGCACC CCCCAATGTG CTCTGGCAGA CGCCATTGGC TTGGCAGAAC
    CCCTCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCACGTCA GAGCCCTCCA
    GCTAGGCAGA CCCCACCAGC CTGGCAGAAC CCAGTCGCTT GGCAGAACCC AGTGATTTGG
    CCAAACCCAG TAATCTGGCA GAACCCAGTG ATCTGGCCAA ACCCCATTGT CTGGCCCGGC
    CCTGTTGTCT GGCCGAATCC ACTGGCCTGG CAGAATCCAC CTGGATGGCA GACTCCACCT
    GGATGGCAGA CCCCACCGGG CTGGCAGGGT CCTCCAGACT GGCAAGGTCC TCCTGACTGG
    CCGCTACCAC CCGACTGGCC ACTGCCACCT GATTGGCCAC TTCCCACTGA CTGGCCACTA
    CCACCTGACT GGATCCCCGC TGATTGGCCA ATTCCACCTG ACTGGCAGAA CCTGCGCCCC
    TCGCCTAACC TGCGCCCTTC TCCCAACTCG CGTGCCTCAC AGAACCCAGG TGCTGCACAG
    CCCCGAGATG TGGCCCTTCT TCAGGAAAGA GCAAATAAGT TGGTCAAGTA CTTGATGCTT
    AAGGACTACA CAAAGGTGCC CATCAAGCGC TCAGAAATGC TGAGAGATAT CATCCGTGAA
    TACACTGATG TTTATCCAGA AATCATTGAA CGTGCATGCT TTGTCCTAGA GAAGAAATTT
    GGGATTCAAC TGAAAGAAAT TGACAAAGAA GAACACCTGT ATATTCTCAT CAGTACCCCC
    GAGTCCCTGG CTGGCATACT GGGAACGACC AAAGACACAC CCAAGCTCGG TCTCCTCTTG
    GTGATTCTGG GTGTCATCTT CATGAATGGC AACCGTGCCA GTGAGGCTGT CCTCTGGGAG
    GCACTACGCA AGATGGGACT GCGTCCTGGG GTGAGACATC CCCTCCTTGG AGATCTAAGG
    AAACTTCTCA CCTATGAGTT TGTAAAGCAG AAATACCTGG ACTACAGACG AGTGCCCAAC
    AGCAACCCCC CGGAGTATGA GTTCCTCTGG GGCCTCCGTT CCTACCATGA GACTAGCAAG
    ATGAAAGTGC TGAGATTCAT TGCAGAGGTT CAGAAAAGAG ACCCTCGTGA CTGGACTGCA
    CAGTTCATGG AGGCTGCAGA TGAGGCCTTG GATGCTCTGG ATGCTGCTGC AGCTGAGGCC
    GAAGCCCGGG CTGAAGCAAG AACCCGCATG GGAATTGGAG ATGAGGCTGT GTCTGGGCCC
    TGGAGCTGGG ATGACATTGA GTTTGAGCTG CTGACCTGGG ATGAGGAAGG AGATTTTGGA
    GATCCCTGGT CCAGAATTCC ATTTACCTTC TGGGCCAGAT ACCACCAGAA TGCCCGCTCC
    AGATTCCCTC AGACCTTTGC CGGTCCCATT ATTGGTCCTG GTGGTACAGC CAGTGCCAAC
    TTCGCTGCCA ACTTTGGTGC CATTGGTTTC TTCTGGGTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001005333.1
    CDS170..2674
    Translation

    Target ORF information:

    RefSeq Version NM_001005333.2
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001005333.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGAATCC TGACGCTTGT 
    AGAGCAGTCT GTCACCCCCT CCCCCAGCCT CCTGCCTCCA CCCTCCCTCT TTCTGCATTC
    CCCACTCTGT GCGACCCCCC TTATTCTCAA CTCCGCGATC CGCCTGCCGT CCTGAGCTGC
    TACTGCACGC CTCTTGGAGC GTCCCCGGCT CCTGCTGAGG CCTCCGTAGA AGACAGCGCC
    TTGCTTATGC AGACCTTGAT GGAGGCCATC CAGATCTCAG AGGCTCCACC TACTAACCAG
    GCCACCGCAG CTGCTAGTCC CCAGAGTTCA CAGCCCCCAA CTGCCAATGA GATGGCTGAC
    ATTCAGGTTT CAGCAGCTGC CGCTAGGCCT AAGTCAGCCT TTAAAGTCCA GAATGCCACC
    ACAAAAGGCC CAAATGGTGT CTATGATTTC TCTCAGGCTC ATAATGCCAA GGATGTGCCC
    AACACGCAGC CCAAGGCAGC CTTTAAGTCC CAAAATGCTA CCCCAAAGGG TCCAAATGCT
    GCCTATGATT TTTCCCAGGC AGCAACCACT GGTGAGTTAG CTGCTAACAA GTCTGAGATG
    GCCTTCAAGG CCCAGAATGC CACTACTAAA GTGGGCCCAA ATGCCACCTA CAATTTCTCT
    CAGTCTCTCA ATGCCAATGA CCTGGCCAAC AGCAGGCCTA AGACCCCTTT CAAGGCTTGG
    AATGATACCA CTAAGGCCCC AACAGCTGAT ACCCAGACCC AGAATGTAAA TCAGGCCAAA
    ATGGCCACTT CCCAGGCTGA CATAGAGACC GACCCAGGTA TCTCTGAACC TGACGGTGCA
    ACTGCACAGA CATCAGCAGA TGGTTCCCAG GCTCAGAATC TGGAGTCCCG GACAATAATT
    CGGGGCAAGA GGACCCGCAA GATTAATAAC TTGAATGTTG AAGAGAACAG CAGTGGGGAT
    CAGAGGCGGG CCCCACTGGC TGCAGGGACC TGGAGGTCTG CACCAGTTCC AGTGACCACT
    CAGAACCCAC CTGGCGCACC CCCCAATGTG CTCTGGCAGA CGCCATTGGC TTGGCAGAAC
    CCCTCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCACGTCA GAGCCCTCCA
    GCTAGGCAGA CCCCACCAGC CTGGCAGAAC CCAGTCGCTT GGCAGAACCC AGTGATTTGG
    CCAAACCCAG TAATCTGGCA GAACCCAGTG ATCTGGCCAA ACCCCATTGT CTGGCCCGGC
    CCTGTTGTCT GGCCGAATCC ACTGGCCTGG CAGAATCCAC CTGGATGGCA GACTCCACCT
    GGATGGCAGA CCCCACCGGG CTGGCAGGGT CCTCCAGACT GGCAAGGTCC TCCTGACTGG
    CCGCTACCAC CCGACTGGCC ACTGCCACCT GATTGGCCAC TTCCCACTGA CTGGCCACTA
    CCACCTGACT GGATCCCCGC TGATTGGCCA ATTCCACCTG ACTGGCAGAA CCTGCGCCCC
    TCGCCTAACC TGCGCCCTTC TCCCAACTCG CGTGCCTCAC AGAACCCAGG TGCTGCACAG
    CCCCGAGATG TGGCCCTTCT TCAGGAAAGA GCAAATAAGT TGGTCAAGTA CTTGATGCTT
    AAGGACTACA CAAAGGTGCC CATCAAGCGC TCAGAAATGC TGAGAGATAT CATCCGTGAA
    TACACTGATG TTTATCCAGA AATCATTGAA CGTGCATGCT TTGTCCTAGA GAAGAAATTT
    GGGATTCAAC TGAAAGAAAT TGACAAAGAA GAACACCTGT ATATTCTCAT CAGTACCCCC
    GAGTCCCTGG CTGGCATACT GGGAACGACC AAAGACACAC CCAAGCTCGG TCTCCTCTTG
    GTGATTCTGG GTGTCATCTT CATGAATGGC AACCGTGCCA GTGAGGCTGT CCTCTGGGAG
    GCACTACGCA AGATGGGACT GCGTCCTGGG GTGAGACATC CCCTCCTTGG AGATCTAAGG
    AAACTTCTCA CCTATGAGTT TGTAAAGCAG AAATACCTGG ACTACAGACG AGTGCCCAAC
    AGCAACCCCC CGGAGTATGA GTTCCTCTGG GGCCTCCGTT CCTACCATGA GACTAGCAAG
    ATGAAAGTGC TGAGATTCAT TGCAGAGGTT CAGAAAAGAG ACCCTCGTGA CTGGACTGCA
    CAGTTCATGG AGGCTGCAGA TGAGGCCTTG GATGCTCTGG ATGCTGCTGC AGCTGAGGCC
    GAAGCCCGGG CTGAAGCAAG AACCCGCATG GGAATTGGAG ATGAGGCTGT GTCTGGGCCC
    TGGAGCTGGG ATGACATTGA GTTTGAGCTG CTGACCTGGG ATGAGGAAGG AGATTTTGGA
    GATCCCTGGT CCAGAATTCC ATTTACCTTC TGGGCCAGAT ACCACCAGAA TGCCCGCTCC
    AGATTCCCTC AGACCTTTGC CGGTCCCATT ATTGGTCCTG GTGGTACAGC CAGTGCCAAC
    TTCGCTGCCA ACTTTGGTGC CATTGGTTTC TTCTGGGTTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version NM_006986.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-15
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX422007.2, CD387872.1, BF529661.1, BC014070.2 and AF132205.1. On Sep 24, 2004 this sequence version replaced NM_006986.2. Transcript Variant: This variant (2) lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF258554.1, SRR1803612.173417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008917.3
    CDS191..2527
    Translation

    Target ORF information:

    RefSeq Version NM_006986.3
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006986.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version NM_001005332.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG819042.1 and BC014070.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001005332.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.27158.1, SRR1803614.248884.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001005332.1
    CDS79..2415
    Translation

    Target ORF information:

    RefSeq Version NM_001005332.2
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001005332.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version NM_006986.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF529661.1 and BC014070.2. On Jun 2, 2019 this sequence version replaced NM_006986.3. Transcript Variant: This variant (2) lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF258554.1, SRR7346977.1314785.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008917.3
    CDS170..2506
    Translation

    Target ORF information:

    RefSeq Version NM_006986.4
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006986.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version NM_001005332.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-15
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG819042.1, BC014070.2 and AF132205.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.907521.1, SRR3476690.318773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001005332.1
    CDS96..2432
    Translation

    Target ORF information:

    RefSeq Version NM_001005332.1
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001005332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version XM_017029978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885467.1
    CDS106..2442
    Translation

    Target ORF information:

    RefSeq Version XM_017029978.1
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version XM_011530835.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011529137.1
    CDS214..2550
    Translation

    Target ORF information:

    RefSeq Version XM_011530835.2
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530835.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version XM_017029979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885468.1
    CDS792..3128
    Translation

    Target ORF information:

    RefSeq Version XM_017029979.1
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version XM_011530835.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product melanoma-associated antigen D1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011529137.1
    Translation

    Target ORF information:

    RefSeq Version XM_011530835.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530835.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18181
    Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , NM_001005332.2 , NM_006986.4 , NM_001005332.1 , XM_011530835.1 , XM_017029980.1
    Accession Version XM_017029980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product melanoma-associated antigen D1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885469.1
    CDS610..2946
    Translation

    Target ORF information:

    RefSeq Version XM_017029980.1
    Organism Homo sapiens(human)
    Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
    GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
    CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
    GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
    CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
    AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
    GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
    AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
    TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
    TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
    AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
    CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
    CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
    AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
    CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
    GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
    CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
    CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
    GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
    CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
    CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
    GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
    AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
    GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
    TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
    ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
    GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
    ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
    GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
    GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
    GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
    CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
    GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
    GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
    GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
    GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
    GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
    AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
    GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.