COL6A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A1
Entrez Gene ID	511422
Full Name	collagen type VI alpha 1 chain
General protein information	Preferred Names collagen type VI alpha 1 chain Names collagen alpha-1(VI) chain collagen, type VI, alpha 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 1 Map Location: 1q12-q14

mRNA and Protein(s)

mRNA	Protein	Name
NM_001143865.1	NP_001137337.1	collagen alpha-1(VI) chain precursor
NM_001143865.2	NP_001137337.1	collagen alpha-1(VI) chain precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col6a1	XP_215375.5
Xenopus tropicalis (tropical clawed frog)	col6a1	XP_002932077.2
Canis lupus familiaris (dog)	COL6A1	XP_003434049.2
Mus musculus (house mouse)	Col6a1	NP_034063.1
Gallus gallus (chicken)	COL6A1	NP_990438.1
Danio rerio (zebrafish)	col6a1	XP_698253.3
Homo sapiens (human)	COL6A1	NP_001839.2
Pan troglodytes (chimpanzee)	COL6A1	XP_001158445.1
	LOC721855	XP_001118050.2
Bos taurus (cattle)	COL6A1	NP_001137337.1

Gene Ontology
Bibliography

Related articles in PubMed

Type II and VI collagen in nasal and articular cartilage and the effect of IL-1alpha on the distribution of these collagens.
Jansen ID, Hollander AP, Buttle DJ, Everts V
Journal of molecular histology41(1)9-17(2010 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The distribution of type II and VI collagen was immunocytochemically investigated in bovine articular and nasal cartilage.
Title: Type II and VI collagen in nasal and articular cartilage and the effect of IL-1alpha on the distribution of these collagens.

The following COL6A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa239072	NM_001143865.2 Latest version!	Bos taurus collagen type VI alpha 1 chain (COL6A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa39810	NM_001143865.1 Latest version!	Bos taurus collagen type VI alpha 1 chain (COL6A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A1 ( NM_001143865.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137337.1 Bos taurus collagen type VI alpha 1 chain (COL6A1), mRNA.

Price & Availability↑

CloneID	OBa239072
Clone ID Related Accession (Same CDS sequence)	NM_001143865.2
Accession Version	NM_001143865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	collagen alpha-1(VI) chain precursor
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000001.1. On May 30, 2018 this sequence version replaced NM_001143865.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5765710.16199.1, SRR5571257.47223.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCTGC CCCGCGCTCT GCTCCCCCTG CTGCTGCAGG CCTGCTGGGC CTCCGCGCAG 
GACGACCCCG TGGCCTCAAG GGCCATCGCT TTCCAAGACT GCCCTGTGGA CCTGTTCTTT 
GTGCTGGACA CCTCCGAGAG CGTGGCCTTG AGGCTGAAGC CCTATGGGGC CCTGGTGGAC 
AAGGTCAAGT CCTTCACCAA GCGCTTCATT GACAACCTGA ACGACAGGTA CTACCGCTGT 
GACCGCAACC TGGTGTGGAA TGCGGGCGCG CTGCACTATA GCGACGAAGT GGAGATCATC 
CGCGGGCTCA CGCGCATGCC CAGCGGCCGC GACGAGCTTA AGAGCAGCGT GGACGCCGTC 
AAGTACTTCG GAAAGGGCAC CTATACCGAC TGCGCCATCA AGAAGGGTCT GGAGGAGCTG 
CTCGTGGGGG GCTCCCACCT AAAGGAGAAC AAGTACTTGG TTGTGGTGAC TGACGGGCAC 
CCCCTGGAGG GCTACAAGGA GCCCTGCGGG GGCCTGGAGG ATGCTGTGAA CGAGGCCAAG 
CATCTGGGCA TCAAAGTCTT CTCCGTGGCC ATCACGCCCG ACCACCTGGA GCCACGTCTG 
AGCATCATCG CCACGGACCA CACGTACCGG CGCAACTTCA CGGCGGCTGA CTGGGGGCAG 
AGCCGCGACG CCGAGGAAGT CATCAGCCAG ACCATCGACA CCATCACCGA CATGATCAAA 
AATAATGTGG AGCAAGTGTG CTGCTCCTTC GAGTGCCAGC CTGCCAGAGG ACCCCCCGGG 
CCTCGAGGCG ACCCCGGGTA CGAGGGAGAA AGAGGGAAGC CGGGGCTCCC GGGAGAGAAA 
GGAGAAGCTG GAGACCCTGG AAGACCTGGG GACCTTGGAC CCGTCGGCTA CCAGGGCATG 
AAGGGAGAAA AAGGGAGCCG AGGGGAGAAG GGCTCCAGGG GACCCAAAGG CTACAAGGGC 
GAGAAGGGAA AGCGTGGCAT GGACGGCGTG GATGGCATGA AGGGGGAGAC AGGGTTCCCT 
GGCCTGCCAG GCTGCAAGGG CTCGCCCGGA TTCGATGGTA TCCAAGGACC CCCCGGGCCC 
AAGGGTGACC CAGGTGCCTT CGGACTGAAA GGACAGAAGG GTGAGCCCGG AGCAGACGGG 
GAGCCTGGGA GGCCCGGGAG CACGGGGCCG CCTGGAGATG AGGGTGAGCC TGGAGAGCCC 
GGTCCCCCGG GAGAGAAGGG AGAAGCCGGC GATGAGGGAA ATGCAGGACC AGACGGAGCC 
CCCGGAGAGA GGGGCGGCCC TGGGGAAAGA GGACCTCGAG GGACCCCAGG TGCACGGGGC 
CCTAGAGGAG ACCCGGGTGA AGCTGGACCC CAAGGTGACC AGGGACGAGA AGGCCCCGTC 
GGTGTCCCCG GTGACCCGGG CGAGGCTGGC CCCATTGGGC CGAAAGGATA CCGAGGTGAT 
GAGGGACCCC CTGGGACCGA GGGCCCCAAA GGAGCTCCGG GGCCTGCAGG CCCCCCCGGA 
GACCCCGGGC TGATGGGTGA GAGGGGTGAA GACGGCCCCC CCGGGAATGG CACTGAAGGC 
TTCCCCGGCT TTCCCGGCTA TCCAGGCAGC AGAGGTCCTC CTGGGATAAA CGGCACCAAA 
GGCTACCCTG GCCTCAAGGG GGACGAGGGA GAAGCCGGGG ACCCCGGAGA GGACAATAAT 
GACATTGCTG CACGAGGCGC CAAAGGAGCA AAGGGCTACC GAGGTCCTGA AGGCCCCCAG 
GGACCCCCAG GACACGTGGG ACCACCGGGG CCAGACGAAT GCGAGATTTT GGACATCATC 
ATGAAAATGT GCTCTTGCTG TGAGTGCAAG TGCGGGCCCA TCGACATCCT CTTCGTGCTG 
GACAGCTCTG AGAGCATCGG CCTACAGAAC TTCGAGATCG CCAAGGACTT CATCGTCAAG 
GTCATTGACC GGCTGAGCAA GGATGAGCTG GTCAAGTTTG AGCCTGGGCA GTCGCATGCA 
GGCGTGGTGC AGTACAGCCA CAACCAGATG CAGGAGCACG TGGACCTGAG GGACCCCAAC 
ATCAGGAATG CCCAGGACCT CAAGGAGGCC ATCAAGAAGC TGCAGTGGAT GGGCGGCGGC 
ACCTTCACGG GCGAGGCCCT GCAGTACACC CGGAGCCGGC TGCTGCCACC CACTCCGAAC 
AACCGCATTG CCCTGGTCAT CACCGATGGC CGCTCAGACA CCCAGAGGGA CACCACCCCA 
CTCAGCGTGC TCTGCGGCCC TGACATCCAG GTGGTCTCTG TGGGCATCAA GGATGTCTTT 
GGCTTGGCTG CGGGCTCCGA CCAGCTCAAC GTCATCTCCT GCCAAGGCCT GGCACCCCAG 
GGACGGCCGG GCATCTCATT GGTCAAGGAA AACTACGCCG AGCTTCTGGA CGATGGCTTC 
CTGAAGAACA TCACTGCCCA GATCTGCATA GACAAGAAAT GTCCAGATTA CAGCTGCCCA 
ATCACCTTCT CCTCCCCGGC CGACATCACC ATCCTCCTGG ACGGCTCCGC CAGCGTGGGC 
AGCCACAACT TTGACATCAC CAAACGCTTT GCCAAGCGGC TGGCAGAGCG CTTCCTGACA 
GCGAGCCGGA CAGACCCGGG CCAGGACGTG CGTGTGGCAG TGGTGCAGTA CAGCGGTACG 
GGGCAGCAGC GGCCGGAGCG CGCGGCCCTG CAGTTCCTGC AGAACTACAC CGTGCTGGCC 
AACACCGTGG ACTCCATGGA CTTCTTCAAT GACGCCACCG ACGTCATGGA CGCCCTGGGC 
TACGTGACCC GCTTCTACCG CGAGGCCTCG TCCAACGCCG CCAAGAAGAG GCTGCTGCTC 
TTCTCGGATG GCAACTCGCA AGGTGCCACG CCGGCCGCCA TCGAGAAGGC GGTGCAGGAG 
GCCCAGCGGG CGGGCGTGGA GATCTTCGCG GTGGTGGTGG GCCGCCAGGT GAACGAGCCC 
CACGTGCGTG TCCTGGTCAC TGGCAAGGCG GCTGAGTATG ACGTGGTCTT CGGCGAGCGC 
CACCTGTTCC GCGTGCCCAG TTACCAGGCG TTGCTGCGGG GCGTCTTCTA CCAGACGGTG 
TCCAGGAAGG TGGCGCTGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137337.1
CDS	220..3303
Translation	MRLPRALLPLLLQACWASAQDDPVASRAIAFQDCPVDLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLNDRYYRCDRNLVWNAGALHYSDEVEIIRGLTRMPSGRDELKSSVDAVKYFGKGTYTDCAIKKGLEELLVGGSHLKENKYLVVVTDGHPLEGYKEPCGGLEDAVNEAKHLGIKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEVISQTIDTITDMIKNNVEQVCCSFECQPARGPPGPRGDPGYEGERGKPGLPGEKGEAGDPGRPGDLGPVGYQGMKGEKGSRGEKGSRGPKGYKGEKGKRGMDGVDGMKGETGFPGLPGCKGSPGFDGIQGPPGPKGDPGAFGLKGQKGEPGADGEPGRPGSTGPPGDEGEPGEPGPPGEKGEAGDEGNAGPDGAPGERGGPGERGPRGTPGARGPRGDPGEAGPQGDQGREGPVGVPGDPGEAGPIGPKGYRGDEGPPGTEGPKGAPGPAGPPGDPGLMGERGEDGPPGNGTEGFPGFPGYPGSRGPPGINGTKGYPGLKGDEGEAGDPGEDNNDIAARGAKGAKGYRGPEGPQGPPGHVGPPGPDECEILDIIMKMCSCCECKCGPIDILFVLDSSESIGLQNFEIAKDFIVKVIDRLSKDELVKFEPGQSHAGVVQYSHNQMQEHVDLRDPNIRNAQDLKEAIKKLQWMGGGTFTGEALQYTRSRLLPPTPNNRIALVITDGRSDTQRDTTPLSVLCGPDIQVVSVGIKDVFGLAAGSDQLNVISCQGLAPQGRPGISLVKENYAELLDDGFLKNITAQICIDKKCPDYSCPITFSSPADITILLDGSASVGSHNFDITKRFAKRLAERFLTASRTDPGQDVRVAVVQYSGTGQQRPERAALQFLQNYTVLANTVDSMDFFNDATDVMDALGYVTRFYREASSNAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGVEIFAVVVGRQVNEPHVRVLVTGKAAEYDVVFGERHLFRVPSYQALLRGVFYQTVSRKVALD

Target ORF information:

RefSeq Version	NM_001143865.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 1 chain (COL6A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143865.2

ORF Insert Sequence:

ATGAGGCTGC CCCGCGCTCT GCTCCCCCTG CTGCTGCAGG CCTGCTGGGC CTCCGCGCAG 
GACGACCCCG TGGCCTCAAG GGCCATCGCT TTCCAAGACT GCCCTGTGGA CCTGTTCTTT 
GTGCTGGACA CCTCCGAGAG CGTGGCCTTG AGGCTGAAGC CCTATGGGGC CCTGGTGGAC 
AAGGTCAAGT CCTTCACCAA GCGCTTCATT GACAACCTGA ACGACAGGTA CTACCGCTGT 
GACCGCAACC TGGTGTGGAA TGCGGGCGCG CTGCACTATA GCGACGAAGT GGAGATCATC 
CGCGGGCTCA CGCGCATGCC CAGCGGCCGC GACGAGCTTA AGAGCAGCGT GGACGCCGTC 
AAGTACTTCG GAAAGGGCAC CTATACCGAC TGCGCCATCA AGAAGGGTCT GGAGGAGCTG 
CTCGTGGGGG GCTCCCACCT AAAGGAGAAC AAGTACTTGG TTGTGGTGAC TGACGGGCAC 
CCCCTGGAGG GCTACAAGGA GCCCTGCGGG GGCCTGGAGG ATGCTGTGAA CGAGGCCAAG 
CATCTGGGCA TCAAAGTCTT CTCCGTGGCC ATCACGCCCG ACCACCTGGA GCCACGTCTG 
AGCATCATCG CCACGGACCA CACGTACCGG CGCAACTTCA CGGCGGCTGA CTGGGGGCAG 
AGCCGCGACG CCGAGGAAGT CATCAGCCAG ACCATCGACA CCATCACCGA CATGATCAAA 
AATAATGTGG AGCAAGTGTG CTGCTCCTTC GAGTGCCAGC CTGCCAGAGG ACCCCCCGGG 
CCTCGAGGCG ACCCCGGGTA CGAGGGAGAA AGAGGGAAGC CGGGGCTCCC GGGAGAGAAA 
GGAGAAGCTG GAGACCCTGG AAGACCTGGG GACCTTGGAC CCGTCGGCTA CCAGGGCATG 
AAGGGAGAAA AAGGGAGCCG AGGGGAGAAG GGCTCCAGGG GACCCAAAGG CTACAAGGGC 
GAGAAGGGAA AGCGTGGCAT GGACGGCGTG GATGGCATGA AGGGGGAGAC AGGGTTCCCT 
GGCCTGCCAG GCTGCAAGGG CTCGCCCGGA TTCGATGGTA TCCAAGGACC CCCCGGGCCC 
AAGGGTGACC CAGGTGCCTT CGGACTGAAA GGACAGAAGG GTGAGCCCGG AGCAGACGGG 
GAGCCTGGGA GGCCCGGGAG CACGGGGCCG CCTGGAGATG AGGGTGAGCC TGGAGAGCCC 
GGTCCCCCGG GAGAGAAGGG AGAAGCCGGC GATGAGGGAA ATGCAGGACC AGACGGAGCC 
CCCGGAGAGA GGGGCGGCCC TGGGGAAAGA GGACCTCGAG GGACCCCAGG TGCACGGGGC 
CCTAGAGGAG ACCCGGGTGA AGCTGGACCC CAAGGTGACC AGGGACGAGA AGGCCCCGTC 
GGTGTCCCCG GTGACCCGGG CGAGGCTGGC CCCATTGGGC CGAAAGGATA CCGAGGTGAT 
GAGGGACCCC CTGGGACCGA GGGCCCCAAA GGAGCTCCGG GGCCTGCAGG CCCCCCCGGA 
GACCCCGGGC TGATGGGTGA GAGGGGTGAA GACGGCCCCC CCGGGAATGG CACTGAAGGC 
TTCCCCGGCT TTCCCGGCTA TCCAGGCAGC AGAGGTCCTC CTGGGATAAA CGGCACCAAA 
GGCTACCCTG GCCTCAAGGG GGACGAGGGA GAAGCCGGGG ACCCCGGAGA GGACAATAAT 
GACATTGCTG CACGAGGCGC CAAAGGAGCA AAGGGCTACC GAGGTCCTGA AGGCCCCCAG 
GGACCCCCAG GACACGTGGG ACCACCGGGG CCAGACGAAT GCGAGATTTT GGACATCATC 
ATGAAAATGT GCTCTTGCTG TGAGTGCAAG TGCGGGCCCA TCGACATCCT CTTCGTGCTG 
GACAGCTCTG AGAGCATCGG CCTACAGAAC TTCGAGATCG CCAAGGACTT CATCGTCAAG 
GTCATTGACC GGCTGAGCAA GGATGAGCTG GTCAAGTTTG AGCCTGGGCA GTCGCATGCA 
GGCGTGGTGC AGTACAGCCA CAACCAGATG CAGGAGCACG TGGACCTGAG GGACCCCAAC 
ATCAGGAATG CCCAGGACCT CAAGGAGGCC ATCAAGAAGC TGCAGTGGAT GGGCGGCGGC 
ACCTTCACGG GCGAGGCCCT GCAGTACACC CGGAGCCGGC TGCTGCCACC CACTCCGAAC 
AACCGCATTG CCCTGGTCAT CACCGATGGC CGCTCAGACA CCCAGAGGGA CACCACCCCA 
CTCAGCGTGC TCTGCGGCCC TGACATCCAG GTGGTCTCTG TGGGCATCAA GGATGTCTTT 
GGCTTGGCTG CGGGCTCCGA CCAGCTCAAC GTCATCTCCT GCCAAGGCCT GGCACCCCAG 
GGACGGCCGG GCATCTCATT GGTCAAGGAA AACTACGCCG AGCTTCTGGA CGATGGCTTC 
CTGAAGAACA TCACTGCCCA GATCTGCATA GACAAGAAAT GTCCAGATTA CAGCTGCCCA 
ATCACCTTCT CCTCCCCGGC CGACATCACC ATCCTCCTGG ACGGCTCCGC CAGCGTGGGC 
AGCCACAACT TTGACATCAC CAAACGCTTT GCCAAGCGGC TGGCAGAGCG CTTCCTGACA 
GCGAGCCGGA CAGACCCGGG CCAGGACGTG CGTGTGGCAG TGGTGCAGTA CAGCGGTACG 
GGGCAGCAGC GGCCGGAGCG CGCGGCCCTG CAGTTCCTGC AGAACTACAC CGTGCTGGCC 
AACACCGTGG ACTCCATGGA CTTCTTCAAT GACGCCACCG ACGTCATGGA CGCCCTGGGC 
TACGTGACCC GCTTCTACCG CGAGGCCTCG TCCAACGCCG CCAAGAAGAG GCTGCTGCTC 
TTCTCGGATG GCAACTCGCA AGGTGCCACG CCGGCCGCCA TCGAGAAGGC GGTGCAGGAG 
GCCCAGCGGG CGGGCGTGGA GATCTTCGCG GTGGTGGTGG GCCGCCAGGT GAACGAGCCC 
CACGTGCGTG TCCTGGTCAC TGGCAAGGCG GCTGAGTATG ACGTGGTCTT CGGCGAGCGC 
CACCTGTTCC GCGTGCCCAG TTACCAGGCG TTGCTGCGGG GCGTCTTCTA CCAGACGGTG 
TCCAGGAAGG TGGCGCTGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A1 ( NM_001143865.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137337.1 Bos taurus collagen type VI alpha 1 chain (COL6A1), mRNA.

Price & Availability↑

CloneID	OBa39810
Clone ID Related Accession (Same CDS sequence)	NM_001143865.1
Accession Version	NM_001143865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	collagen alpha-1(VI) chain precursor
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from DAAA02003502.1. On Jan 8, 2009 this sequence version replaced XM_588755.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCTGC CCCGCGCTCT GCTCCCCCTG CTGCTGCAGG CCTGCTGGGC CTCCGCGCAG 
GACGACCCCG TGGCCTCAAG GGCCATCGCC TTCCAAGACT GCCCTGTGGA CCTGTTCTTT 
GTGCTGGACA CCTCCGAGAG CGTGGCCTTG AGGCTGAAGC CCTATGGGGC CCTGGTGGAC 
AAGGTCAAGT CCTTCACCAA GCGCTTCATT GACAACCTGA ACGACAGGTA CTACCGCTGT 
GACCGCAACC TGGTGTGGAA TGCGGGCGCG CTGCACTATA GCGACGAAGT GGAGATCATC 
CGCGGGCTCA CGCGCATGCC CAGCGGCCGC GACGAGCTTA AGAGCAGCGT GGACGCCGTC 
AAGTACTTCG GAAAGGGCAC CTATACCGAC TGCGCCATCA AGAAGGGTCT GGAGGAGCTG 
CTCGTGGGGG GCTCCCACCT AAAGGAGAAC AAGTACTTGG TTGTGGTGAC TGACGGGCAC 
CCCCTGGAGG GCTACAAGGA GCCCTGCGGG GGCCTGGAGG ATGCTGTGAA CGAGGCCAAG 
CATCTGGGCA TCAAAGTCTT CTCCGTGGCC ATCACGCCCG ACCACCTGGA GCCACGTCTG 
AGCATCATCG CCACGGACCA CACGTACCGG CGCAACTTCA CGGCGGCTGA CTGGGGGCAG 
AGCCGCGACG CCGAGGAAGT CATCAGCCAG ACCATCGACA CCATCACCGA CATGATCAAA 
AATAATGTGG AGCAAGTGTG CTGCTCCTTC GAGTGCCAGC CTGCCAGAGG ACCCCCCGGG 
CCTCGAGGCG ACCCCGGGTA CGAGGGAGAA AGAGGGAAGC CGGGGCTCCC GGGAGAGAAA 
GGAGAAGCTG GAGACCCTGG AAGACCTGGG GACCTTGGAC CCGTCGGCTA CCAGGGCATG 
AAGGGAGAAA AAGGGAGCCG AGGGGAGAAG GGCTCCAGGG GACCCAAAGG CTACAAGGGC 
GAGAAGGGAA AGCGTGGCAT GGACGGCGTG GATGGCATGA AGGGGGAGAC AGGGTTCCCT 
GGCCTGCCAG GCTGCAAGGG CTCGCCCGGA TTCGATGGTA TCCAAGGACC CCCCGGGCCC 
AAGGGTGACC CAGGTGCCTT CGGACTGAAA GGACAGAAGG GTGAGCCCGG AGCAGACGGG 
GAGCCTGGGA GGCCCGGGAG CACGGGGCCG CCTGGAGATG AGGGTGAGCC TGGAGAGCCC 
GGTCCCCCGG GAGAGAAGGG AGAAGCCGGC GATGAGGGAA ATGCAGGACC AGACGGAGCC 
CCCGGAGAGA GGGGCGGCCC TGGGGAAAGA GGACCTCGAG GGACCCCAGG TGCACGGGGC 
CCTAGAGGAG ACCCGGGTGA AGCTGGACCC CAAGGTGACC AGGGACGAGA AGGCCCCGTC 
GGTGTCCCCG GTGACCCGGG CGAGGCTGGC CCCATTGGGC CGAAAGGATA CCGAGGTGAT 
GAGGGACCCC CTGGGACCGA GGGCCCCAAA GGAGCTCCGG GGCCTGCAGG CCCCCCCGGA 
GACCCCGGGC TGATGGGTGA GAGGGGTGAA GACGGCCCCC CCGGGAATGG CACTGAAGGC 
TTCCCCGGCT TTCCCGGCTA TCCAGGCAGC AGAGGTCCTC CTGGGATAAA CGGCACCAAA 
GGCTACCCTG GCCTCAAGGG GGACGAGGGA GAAGCCGGGG ACCCCGGAGA GGACAATAAT 
GACATTGCTG CACGAGGCGC CAAAGGAGCA AAGGGCTACC GAGGTCCTGA AGGCCCCCAG 
GGACCCCCAG GACACGTGGG ACCACCGGGG CCAGACGAAT GCGAGATTTT GGACATCATC 
ATGAAAATGT GCTCTTGCTG TGAGTGCAAG TGCGGGCCCA TCGACATCCT CTTCGTGCTG 
GACAGCTCTG AGAGCATCGG CCTACAGAAC TTCGAGATCG CCAAGGACTT CATCGTCAAG 
GTCATTGACC GGCTGAGCAA GGATGAGCTG GTCAAGTTTG AGCCTGGGCA GTCGCATGCA 
GGCGTGGTGC AGTACAGCCA CAACCAGATG CAGGAGCACG TGGACCTGAG GGACCCCAAC 
ATCAGGAATG CCCAGGACCT CAAGGAGGCC ATCAAGAAGC TGCAGTGGAT GGGCGGCGGC 
ACCTTCACGG GCGAGGCCCT GCAGTACACC CGGAGCCGGC TGCTGCCACC CACTCCGAAC 
AACCGCATTG CCCTGGTCAT CACCGATGGC CGCTCAGACA CCCAGAGGGA CACCACCCCA 
CTCAGCGTGC TCTGCGGCCC TGACATCCAG GTGGTCTCTG TGGGCATCAA GGATGTCTTT 
GGCTTGGCTG CGGGCTCCGA CCAGCTCAAC GTCATCTCCT GCCAAGGCCT GGCACCCCAG 
GGACGGCCGG GCATCTCATT GGTCAAGGAA AACTACGCCG AGCTTCTGGA CGATGGCTTC 
CTGAAGAACA TCACTGCCCA GATCTGCATA GACAAGAAAT GTCCAGATTA CAGCTGCCCA 
ATCACCTTCT CCTCCCCGGC CGACATCACC ATCCTCCTGG ACGGCTCCGC CAGCGTGGGC 
AGCCACAACT TTGACATCAC CAAACGCTTT GCCAAGCGGC TGGCAGAGCG CTTCCTGACA 
GCGAGCCGGA CAGACCCGGG CCAGGACGTG CGTGTGGCAG TGGTGCAGTA CAGCGGTACG 
GGGCAGCAGC GGCCGGAGCG CGCGGCCCTG CAGTTCCTGC AGAACTACAC CGTGCTGGCC 
AACACCGTGG ACTCCATGGA CTTCTTCAAT GACGCCACCG ACGTCATGGA CGCCCTGGGC 
TACGTGACCC GCTTCTACCG CGAGGCCTCG TCCAACGCCG CCAAGAAGAG GCTGCTGCTC 
TTCTCGGATG GCAACTCGCA AGGTGCCACG CCGGCCGCCA TCGAGAAGGC GGTGCAGGAG 
GCCCAGCGGG CGGGCGTGGA GATCTTCGCG GTGGTGGTGG GCCGCCAGGT GAACGAGCCC 
CACGTGCGTG TCCTGGTCAC TGGCAAGGCG GCTGAGTATG ACGTGGTCTT CGGCGAGCGC 
CACCTGTTCC GCGTGCCCAG TTACCAGGCG TTGCTGCGGG GCGTCTTCTA CCAGACGGTG 
TCCAGGAAGG TGGCGCTGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137337.1
CDS	220..3303
Translation	MRLPRALLPLLLQACWASAQDDPVASRAIAFQDCPVDLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLNDRYYRCDRNLVWNAGALHYSDEVEIIRGLTRMPSGRDELKSSVDAVKYFGKGTYTDCAIKKGLEELLVGGSHLKENKYLVVVTDGHPLEGYKEPCGGLEDAVNEAKHLGIKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEVISQTIDTITDMIKNNVEQVCCSFECQPARGPPGPRGDPGYEGERGKPGLPGEKGEAGDPGRPGDLGPVGYQGMKGEKGSRGEKGSRGPKGYKGEKGKRGMDGVDGMKGETGFPGLPGCKGSPGFDGIQGPPGPKGDPGAFGLKGQKGEPGADGEPGRPGSTGPPGDEGEPGEPGPPGEKGEAGDEGNAGPDGAPGERGGPGERGPRGTPGARGPRGDPGEAGPQGDQGREGPVGVPGDPGEAGPIGPKGYRGDEGPPGTEGPKGAPGPAGPPGDPGLMGERGEDGPPGNGTEGFPGFPGYPGSRGPPGINGTKGYPGLKGDEGEAGDPGEDNNDIAARGAKGAKGYRGPEGPQGPPGHVGPPGPDECEILDIIMKMCSCCECKCGPIDILFVLDSSESIGLQNFEIAKDFIVKVIDRLSKDELVKFEPGQSHAGVVQYSHNQMQEHVDLRDPNIRNAQDLKEAIKKLQWMGGGTFTGEALQYTRSRLLPPTPNNRIALVITDGRSDTQRDTTPLSVLCGPDIQVVSVGIKDVFGLAAGSDQLNVISCQGLAPQGRPGISLVKENYAELLDDGFLKNITAQICIDKKCPDYSCPITFSSPADITILLDGSASVGSHNFDITKRFAKRLAERFLTASRTDPGQDVRVAVVQYSGTGQQRPERAALQFLQNYTVLANTVDSMDFFNDATDVMDALGYVTRFYREASSNAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGVEIFAVVVGRQVNEPHVRVLVTGKAAEYDVVFGERHLFRVPSYQALLRGVFYQTVSRKVALD

Target ORF information:

RefSeq Version	NM_001143865.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 1 chain (COL6A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143865.1

ORF Insert Sequence:

ATGAGGCTGC CCCGCGCTCT GCTCCCCCTG CTGCTGCAGG CCTGCTGGGC CTCCGCGCAG 
GACGACCCCG TGGCCTCAAG GGCCATCGCC TTCCAAGACT GCCCTGTGGA CCTGTTCTTT 
GTGCTGGACA CCTCCGAGAG CGTGGCCTTG AGGCTGAAGC CCTATGGGGC CCTGGTGGAC 
AAGGTCAAGT CCTTCACCAA GCGCTTCATT GACAACCTGA ACGACAGGTA CTACCGCTGT 
GACCGCAACC TGGTGTGGAA TGCGGGCGCG CTGCACTATA GCGACGAAGT GGAGATCATC 
CGCGGGCTCA CGCGCATGCC CAGCGGCCGC GACGAGCTTA AGAGCAGCGT GGACGCCGTC 
AAGTACTTCG GAAAGGGCAC CTATACCGAC TGCGCCATCA AGAAGGGTCT GGAGGAGCTG 
CTCGTGGGGG GCTCCCACCT AAAGGAGAAC AAGTACTTGG TTGTGGTGAC TGACGGGCAC 
CCCCTGGAGG GCTACAAGGA GCCCTGCGGG GGCCTGGAGG ATGCTGTGAA CGAGGCCAAG 
CATCTGGGCA TCAAAGTCTT CTCCGTGGCC ATCACGCCCG ACCACCTGGA GCCACGTCTG 
AGCATCATCG CCACGGACCA CACGTACCGG CGCAACTTCA CGGCGGCTGA CTGGGGGCAG 
AGCCGCGACG CCGAGGAAGT CATCAGCCAG ACCATCGACA CCATCACCGA CATGATCAAA 
AATAATGTGG AGCAAGTGTG CTGCTCCTTC GAGTGCCAGC CTGCCAGAGG ACCCCCCGGG 
CCTCGAGGCG ACCCCGGGTA CGAGGGAGAA AGAGGGAAGC CGGGGCTCCC GGGAGAGAAA 
GGAGAAGCTG GAGACCCTGG AAGACCTGGG GACCTTGGAC CCGTCGGCTA CCAGGGCATG 
AAGGGAGAAA AAGGGAGCCG AGGGGAGAAG GGCTCCAGGG GACCCAAAGG CTACAAGGGC 
GAGAAGGGAA AGCGTGGCAT GGACGGCGTG GATGGCATGA AGGGGGAGAC AGGGTTCCCT 
GGCCTGCCAG GCTGCAAGGG CTCGCCCGGA TTCGATGGTA TCCAAGGACC CCCCGGGCCC 
AAGGGTGACC CAGGTGCCTT CGGACTGAAA GGACAGAAGG GTGAGCCCGG AGCAGACGGG 
GAGCCTGGGA GGCCCGGGAG CACGGGGCCG CCTGGAGATG AGGGTGAGCC TGGAGAGCCC 
GGTCCCCCGG GAGAGAAGGG AGAAGCCGGC GATGAGGGAA ATGCAGGACC AGACGGAGCC 
CCCGGAGAGA GGGGCGGCCC TGGGGAAAGA GGACCTCGAG GGACCCCAGG TGCACGGGGC 
CCTAGAGGAG ACCCGGGTGA AGCTGGACCC CAAGGTGACC AGGGACGAGA AGGCCCCGTC 
GGTGTCCCCG GTGACCCGGG CGAGGCTGGC CCCATTGGGC CGAAAGGATA CCGAGGTGAT 
GAGGGACCCC CTGGGACCGA GGGCCCCAAA GGAGCTCCGG GGCCTGCAGG CCCCCCCGGA 
GACCCCGGGC TGATGGGTGA GAGGGGTGAA GACGGCCCCC CCGGGAATGG CACTGAAGGC 
TTCCCCGGCT TTCCCGGCTA TCCAGGCAGC AGAGGTCCTC CTGGGATAAA CGGCACCAAA 
GGCTACCCTG GCCTCAAGGG GGACGAGGGA GAAGCCGGGG ACCCCGGAGA GGACAATAAT 
GACATTGCTG CACGAGGCGC CAAAGGAGCA AAGGGCTACC GAGGTCCTGA AGGCCCCCAG 
GGACCCCCAG GACACGTGGG ACCACCGGGG CCAGACGAAT GCGAGATTTT GGACATCATC 
ATGAAAATGT GCTCTTGCTG TGAGTGCAAG TGCGGGCCCA TCGACATCCT CTTCGTGCTG 
GACAGCTCTG AGAGCATCGG CCTACAGAAC TTCGAGATCG CCAAGGACTT CATCGTCAAG 
GTCATTGACC GGCTGAGCAA GGATGAGCTG GTCAAGTTTG AGCCTGGGCA GTCGCATGCA 
GGCGTGGTGC AGTACAGCCA CAACCAGATG CAGGAGCACG TGGACCTGAG GGACCCCAAC 
ATCAGGAATG CCCAGGACCT CAAGGAGGCC ATCAAGAAGC TGCAGTGGAT GGGCGGCGGC 
ACCTTCACGG GCGAGGCCCT GCAGTACACC CGGAGCCGGC TGCTGCCACC CACTCCGAAC 
AACCGCATTG CCCTGGTCAT CACCGATGGC CGCTCAGACA CCCAGAGGGA CACCACCCCA 
CTCAGCGTGC TCTGCGGCCC TGACATCCAG GTGGTCTCTG TGGGCATCAA GGATGTCTTT 
GGCTTGGCTG CGGGCTCCGA CCAGCTCAAC GTCATCTCCT GCCAAGGCCT GGCACCCCAG 
GGACGGCCGG GCATCTCATT GGTCAAGGAA AACTACGCCG AGCTTCTGGA CGATGGCTTC 
CTGAAGAACA TCACTGCCCA GATCTGCATA GACAAGAAAT GTCCAGATTA CAGCTGCCCA 
ATCACCTTCT CCTCCCCGGC CGACATCACC ATCCTCCTGG ACGGCTCCGC CAGCGTGGGC 
AGCCACAACT TTGACATCAC CAAACGCTTT GCCAAGCGGC TGGCAGAGCG CTTCCTGACA 
GCGAGCCGGA CAGACCCGGG CCAGGACGTG CGTGTGGCAG TGGTGCAGTA CAGCGGTACG 
GGGCAGCAGC GGCCGGAGCG CGCGGCCCTG CAGTTCCTGC AGAACTACAC CGTGCTGGCC 
AACACCGTGG ACTCCATGGA CTTCTTCAAT GACGCCACCG ACGTCATGGA CGCCCTGGGC 
TACGTGACCC GCTTCTACCG CGAGGCCTCG TCCAACGCCG CCAAGAAGAG GCTGCTGCTC 
TTCTCGGATG GCAACTCGCA AGGTGCCACG CCGGCCGCCA TCGAGAAGGC GGTGCAGGAG 
GCCCAGCGGG CGGGCGTGGA GATCTTCGCG GTGGTGGTGG GCCGCCAGGT GAACGAGCCC 
CACGTGCGTG TCCTGGTCAC TGGCAAGGCG GCTGAGTATG ACGTGGTCTT CGGCGAGCGC 
CACCTGTTCC GCGTGCCCAG TTACCAGGCG TTGCTGCGGG GCGTCTTCTA CCAGACGGTG 
TCCAGGAAGG TGGCGCTGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type II and VI collagen in nasal and articular cartilage and the effect of IL-1alpha on the distribution of these collagens.
Journal of molecular histology41(1)9-17(2010 Feb)
Jansen ID,Hollander AP,Buttle DJ,Everts V

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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COL6A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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