BOLA-NC1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BOLA-NC1
Entrez Gene ID	510417
Full Name	non-classical MHC class I antigen
Synonyms	BoLA,BoLA-NC
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005223620.4	XP_005223677.1	non-classical MHC class I antigen isoform X3
XM_005223618.3	XP_005223675.1	non-classical MHC class I antigen isoform X1
XM_005223618.4	XP_005223675.1	non-classical MHC class I antigen isoform X1
XM_024983519.1	XP_024839287.1	non-classical MHC class I antigen isoform X4
NM_001105616.2	NP_001099086.1	non-classical MHC class I antigen precursor
XM_005223620.3	XP_005223677.1	non-classical MHC class I antigen isoform X2
XM_024983518.1	XP_024839286.1	non-classical MHC class I antigen isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DLA-64	NP_001014400.1
Bos taurus (cattle)	BOLA-NC1	NP_001099086.1
Danio rerio (zebrafish)	mhc1uka	XP_005159527.1
Danio rerio (zebrafish)	LOC101883648	XP_005161941.1
Xenopus tropicalis (tropical clawed frog)	LOC100127707	NP_001265363.1
Xenopus tropicalis (tropical clawed frog)	LOC100493954	XP_002940102.2
Xenopus tropicalis (tropical clawed frog)	LOC100495225	XP_002940109.2
Xenopus tropicalis (tropical clawed frog)	LOC100497446	XP_004918996.1
Xenopus tropicalis (tropical clawed frog)	LOC100494770	XP_002940106.1
Xenopus tropicalis (tropical clawed frog)	mhc1b2	NP_001234924.1
Bos taurus (cattle)	BOLA-A	NP_001035644.1
Xenopus tropicalis (tropical clawed frog)	LOC100490553	XP_002938999.2
Xenopus tropicalis (tropical clawed frog)	LOC100497122	XP_004918998.1
Xenopus tropicalis (tropical clawed frog)	LOC100496024	XP_002940114.2
Xenopus tropicalis (tropical clawed frog)	LOC101734387	XP_004918994.1
Xenopus tropicalis (tropical clawed frog)	LOC100495382	XP_002940110.1
Xenopus tropicalis (tropical clawed frog)	LOC100494618	XP_002940105.1
Canis lupus familiaris (dog)	DLA-12	NP_001014401.1
Bos taurus (cattle)	BOLA	NP_001033607.1
Bos taurus (cattle)	JSP.1	NP_001035588.1
Danio rerio (zebrafish)	mhc1uba	NP_571546.1
Xenopus tropicalis (tropical clawed frog)	LOC100217322	XP_004919447.1
Xenopus tropicalis (tropical clawed frog)	LOC100490896	XP_002939001.2
Xenopus tropicalis (tropical clawed frog)	LOC100496639	XP_002940118.2
Xenopus tropicalis (tropical clawed frog)	mr1	XP_002940116.1
Xenopus tropicalis (tropical clawed frog)	LOC100496176	XP_002940115.2
Xenopus tropicalis (tropical clawed frog)	LOC100494130	XP_004918993.1
Xenopus tropicalis (tropical clawed frog)	LOC100494930	XP_004918997.1
Xenopus tropicalis (tropical clawed frog)	LOC101730341	XP_004919000.1
Xenopus tropicalis (tropical clawed frog)	LOC100490378	XP_002938998.1
Xenopus tropicalis (tropical clawed frog)	LOC100487860	XP_002944047.1
Xenopus tropicalis (tropical clawed frog)	LOC100494293	XP_002940103.2
Xenopus tropicalis (tropical clawed frog)	LOC100495081	XP_002940108.1
Xenopus tropicalis (tropical clawed frog)	LOC100494456	XP_002940104.2
Homo sapiens (human)	HLA-B	NP_005505.2
Pan troglodytes (chimpanzee)	PATR-B	NP_001009081.1
	MAMU-B18	NP_001108436.1
Canis lupus familiaris (dog)	DLA88	NP_001014767.1
Danio rerio (zebrafish)	LOC796419	XP_001336749.4
Xenopus tropicalis (tropical clawed frog)	hla-a	NP_001106536.1
Xenopus tropicalis (tropical clawed frog)	LOC100495868	XP_002940113.1
Xenopus tropicalis (tropical clawed frog)	LOC100490728	XP_002939000.1
Xenopus tropicalis (tropical clawed frog)	LOC100495703	XP_002940112.2
Xenopus tropicalis (tropical clawed frog)	LOC100496488	XP_002940117.2
Xenopus tropicalis (tropical clawed frog)	LOC100170421	NP_001123671.1
Xenopus tropicalis (tropical clawed frog)	LOC100170569	XP_004918999.1
Xenopus tropicalis (tropical clawed frog)	LOC100495542	XP_002940111.1
Xenopus tropicalis (tropical clawed frog)	LOC100485167	XP_002935389.2

Gene Ontology
Bibliography

Related articles in PubMed

A novel RNAseq-assisted method for MHC class I genotyping in a non-model species applied to a lethal vaccination-induced alloimmune disease.
Demasius W, Weikard R, Hadlich F, Buitkamp J, K?hn C
BMC genomics17365(2016 05)

Non-classical major histocompatibility complex class makes a crucial contribution to reproduction in the dairy cow.
Shu L, Peng X, Zhang S, Deng G, Wu Y, He M, Li B, Li C, Zhang K
The Journal of reproduction and development58(5)569-75(2012)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

BoLA class I allele diversity and polymorphism in a herd of cattle.
Babiuk S, Horseman B, Zhang C, Bickis M, Kusalik A, Schook LB, Abrahamsen MS, Pontarollo R
Immunogenetics59(2)167-76(2007 Feb)

Evidence for expression of both classical and non-classical major histocompatibility complex class I genes in bovine trophoblast cells.
Davies CJ, Eldridge JA, Fisher PJ, Schlafer DH
American journal of reproductive immunology (New York, N.Y. : 1989)55(3)188-200(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that BoLA-NC1 is up-regulated in first and third trimesters, but not in second trimester; study investigated expression of three non-classical major histocompatibility complex genes in peripheral mononuclear cells of pregnant cows.
Title: Non-classical major histocompatibility complex class makes a crucial contribution to reproduction in the dairy cow.

The following BOLA-NC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BOLA-NC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40423	NM_001105616.2 Latest version!	Bos taurus non-classical MHC class I antigen (BOLA-NC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa32121	XM_005223618.4 Latest version!	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa32123	XM_005223620.4 Latest version!	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa229456	XM_024983518.1 Latest version!	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa229457	XM_024983519.1 Latest version!	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OBa32121	XM_005223618.3	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa32123	XM_005223620.3	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BOLA-NC1 ( NM_001105616.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001099086.1 Bos taurus non-classical MHC class I antigen (BOLA-NC1), mRNA.

Price & Availability↑

CloneID	OBa40423
Clone ID Related Accession (Same CDS sequence)	NM_001105616.2
Accession Version	NM_001105616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	non-classical MHC class I antigen precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC109706.1. On Aug 17, 2015 this sequence version replaced NM_001105616.1. ##Evidence-Data-START## Transcript exon combination :: BC109706.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCA CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCA ACAGGTGCTG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCA AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAT GGCACATGTG ACCCATCACC CCATCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAGCGC 
AATGGGGAGG ACCAGACCCA GGACATGGAG CTTGTGGAGA CCAGGCCTTC AGGGGATGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTT CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGAGCCC CTCACCCTGA GATGGGATAA AGGAGGGGAA 
CCTCCTCAGC CCTCTGTCCC CATCATGGGC ATCATTCTTG TCCTGGTTCT CCTCGTGGTG 
GCTGTGGTGG CTGGAGCTGT GATCTGGAGT AAGAAACGCT CAGGTGAAAA AGGACGGATC 
TACACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099086.1
CDS	8..1036
Translation	MGPRTLLLLLLGVLVPRDTRAGPHSMRYFLTAVSRPGLGEPRFITVGYVDDTQFVRFDSDRPDPRMEPRARWVEDEGPEYWDQETRIQKENTQTFRANLNTLLGYYNQSEAGSHTIQWMHGCGVGSDGRLLRGYNQHAYDGKDYLALNEDLRSWTAADTAAQITKRKWEATGAAERFRNYLEGKCVKLLRRHLENGKDTLLRADPPMAHVTHHPISEREVTLRCWALGFYPEEISLTWQRNGEDQTQDMELVETRPSGDGTFQKWAALAVPSGEEQRYTCRVQHEGLQEPLTLRWDKGGEPPQPSVPIMGIILVLVLLVVAVVAGAVIWSKKRSGEKGRIYT

Target ORF information:

RefSeq Version	NM_001105616.2
Organism	Bos taurus(cattle)
Definition	Bos taurus non-classical MHC class I antigen (BOLA-NC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105616.2

ORF Insert Sequence:

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCA CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCA ACAGGTGCTG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCA AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAT GGCACATGTG ACCCATCACC CCATCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAGCGC 
AATGGGGAGG ACCAGACCCA GGACATGGAG CTTGTGGAGA CCAGGCCTTC AGGGGATGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTT CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGAGCCC CTCACCCTGA GATGGGATAA AGGAGGGGAA 
CCTCCTCAGC CCTCTGTCCC CATCATGGGC ATCATTCTTG TCCTGGTTCT CCTCGTGGTG 
GCTGTGGTGG CTGGAGCTGT GATCTGGAGT AAGAAACGCT CAGGTGAAAA AGGACGGATC 
TACACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA-NC1 ( XM_005223618.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223675.1 Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32121
Clone ID Related Accession (Same CDS sequence)	XM_005223618.3 , XM_005223618.4
Accession Version	XM_005223618.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	non-classical MHC class I antigen isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCT CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCG GCAGGTGAGG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCG AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAA GGCACATGTG GCCCATCACC CCAGCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAACGC 
AATGGGGAGG ACCAGATCCA GGACATGGAG CTTGTGGAAA CCAGGCCTTC AGGGGACGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGGGCCC CTCACCCTGA GATGGGGTGA AAAAGGACGG 
ATCTACACCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCACGGTT 
CCTAAAGAGT CCTGGTCTCA TTCTTCTCTG GGTGATCCCA TCTCTGACAG CAGCAGGGGT 
CACTTTGCCT GTCATTGTCC CCACAAGTCA AGGGATCCAT ACACACAGGA TCTCAGCGGT 
GTCAAGAGGG AAATTTCAGA GCCATCCAGC TCTTGCCATC CTAGAAATTT TGCCGGATTC 
TTCTTCCAAC TTTTCTCCAT CTTTAAAAAG AACCACGTGA TGGAACTTGC AAAGAAGAGG 
GACCCGATAG TTTTCTTATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223675.1
CDS	66..1289
Translation	MGPRTLLLLLLGVLVPRDTRAGPHSMRYFLTAVSRPGLGEPRFITVGYVDDTQFVRFDSDRPDPRMEPRARWVEDEGPEYWDQETRIQKENTQTFRANLNTLLGYYNQSEAGSHTIQWMHGCGVGSDGRLLRGYNQLAYDGKDYLALNEDLRSWTAADTAAQITKRKWEAAGEAERFRNYLEGECVKLLRRHLENGKDTLLRADPPKAHVAHHPSSEREVTLRCWALGFYPEEISLTWQRNGEDQIQDMELVETRPSGDGTFQKWAALAVPSGEEQRYTCRVQHEGLQGPLTLRWGEKGRIYTQASSSDSAQGSDVSLTVPKESWSHSSLGDPISDSSRGHFACHCPHKSRDPYTQDLSGVKREISEPSSSCHPRNFAGFFFQLFSIFKKNHVMELAKKRDPIVFLS

Target ORF information:

RefSeq Version	XM_005223618.3
Organism	Bos taurus(cattle)
Definition	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223618.3

ORF Insert Sequence:

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCT CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCG GCAGGTGAGG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCG AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAA GGCACATGTG GCCCATCACC CCAGCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAACGC 
AATGGGGAGG ACCAGATCCA GGACATGGAG CTTGTGGAAA CCAGGCCTTC AGGGGACGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGGGCCC CTCACCCTGA GATGGGGTGA AAAAGGACGG 
ATCTACACCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCACGGTT 
CCTAAAGAGT CCTGGTCTCA TTCTTCTCTG GGTGATCCCA TCTCTGACAG CAGCAGGGGT 
CACTTTGCCT GTCATTGTCC CCACAAGTCA AGGGATCCAT ACACACAGGA TCTCAGCGGT 
GTCAAGAGGG AAATTTCAGA GCCATCCAGC TCTTGCCATC CTAGAAATTT TGCCGGATTC 
TTCTTCCAAC TTTTCTCCAT CTTTAAAAAG AACCACGTGA TGGAACTTGC AAAGAAGAGG 
GACCCGATAG TTTTCTTATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA-NC1 ( XM_005223618.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223675.1 Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32121
Clone ID Related Accession (Same CDS sequence)	XM_005223618.3 , XM_005223618.4
Accession Version	XM_005223618.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	non-classical MHC class I antigen isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223618.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCT CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCG GCAGGTGAGG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCG AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAA GGCACATGTG GCCCATCACC CCAGCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAACGC 
AATGGGGAGG ACCAGATCCA GGACATGGAG CTTGTGGAAA CCAGGCCTTC AGGGGACGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGGGCCC CTCACCCTGA GATGGGGTGA AAAAGGACGG 
ATCTACACCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCACGGTT 
CCTAAAGAGT CCTGGTCTCA TTCTTCTCTG GGTGATCCCA TCTCTGACAG CAGCAGGGGT 
CACTTTGCCT GTCATTGTCC CCACAAGTCA AGGGATCCAT ACACACAGGA TCTCAGCGGT 
GTCAAGAGGG AAATTTCAGA GCCATCCAGC TCTTGCCATC CTAGAAATTT TGCCGGATTC 
TTCTTCCAAC TTTTCTCCAT CTTTAAAAAG AACCACGTGA TGGAACTTGC AAAGAAGAGG 
GACCCGATAG TTTTCTTATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223675.1
CDS	90..1313
Translation	MGPRTLLLLLLGVLVPRDTRAGPHSMRYFLTAVSRPGLGEPRFITVGYVDDTQFVRFDSDRPDPRMEPRARWVEDEGPEYWDQETRIQKENTQTFRANLNTLLGYYNQSEAGSHTIQWMHGCGVGSDGRLLRGYNQLAYDGKDYLALNEDLRSWTAADTAAQITKRKWEAAGEAERFRNYLEGECVKLLRRHLENGKDTLLRADPPKAHVAHHPSSEREVTLRCWALGFYPEEISLTWQRNGEDQIQDMELVETRPSGDGTFQKWAALAVPSGEEQRYTCRVQHEGLQGPLTLRWGEKGRIYTQASSSDSAQGSDVSLTVPKESWSHSSLGDPISDSSRGHFACHCPHKSRDPYTQDLSGVKREISEPSSSCHPRNFAGFFFQLFSIFKKNHVMELAKKRDPIVFLS

Target ORF information:

RefSeq Version	XM_005223618.4
Organism	Bos taurus(cattle)
Definition	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223618.4

ORF Insert Sequence:

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCT CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCG GCAGGTGAGG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCG AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAA GGCACATGTG GCCCATCACC CCAGCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAACGC 
AATGGGGAGG ACCAGATCCA GGACATGGAG CTTGTGGAAA CCAGGCCTTC AGGGGACGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGGGCCC CTCACCCTGA GATGGGGTGA AAAAGGACGG 
ATCTACACCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCACGGTT 
CCTAAAGAGT CCTGGTCTCA TTCTTCTCTG GGTGATCCCA TCTCTGACAG CAGCAGGGGT 
CACTTTGCCT GTCATTGTCC CCACAAGTCA AGGGATCCAT ACACACAGGA TCTCAGCGGT 
GTCAAGAGGG AAATTTCAGA GCCATCCAGC TCTTGCCATC CTAGAAATTT TGCCGGATTC 
TTCTTCCAAC TTTTCTCCAT CTTTAAAAAG AACCACGTGA TGGAACTTGC AAAGAAGAGG 
GACCCGATAG TTTTCTTATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA-NC1 ( XM_005223620.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223677.1 Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa32123
Clone ID Related Accession (Same CDS sequence)	XM_005223620.4 , XM_005223620.3
Accession Version	XM_005223620.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	non-classical MHC class I antigen isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223620.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCT CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCG GCAGGTGAGG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCG AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAA GGCACATGTG GCCCATCACC CCAGCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAACGC 
AATGGGGAGG ACCAGATCCA GGACATGGAG CTTGTGGAAA CCAGGCCTTC AGGGGACGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGGGCCC CTCACCCTGA GATGGGGTGA AAAAGGACGG 
ATCTACACCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCACGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223677.1
CDS	89..1060
Translation	MGPRTLLLLLLGVLVPRDTRAGPHSMRYFLTAVSRPGLGEPRFITVGYVDDTQFVRFDSDRPDPRMEPRARWVEDEGPEYWDQETRIQKENTQTFRANLNTLLGYYNQSEAGSHTIQWMHGCGVGSDGRLLRGYNQLAYDGKDYLALNEDLRSWTAADTAAQITKRKWEAAGEAERFRNYLEGECVKLLRRHLENGKDTLLRADPPKAHVAHHPSSEREVTLRCWALGFYPEEISLTWQRNGEDQIQDMELVETRPSGDGTFQKWAALAVPSGEEQRYTCRVQHEGLQGPLTLRWGEKGRIYTQASSSDSAQGSDVSLTVPKV

Target ORF information:

RefSeq Version	XM_005223620.4
Organism	Bos taurus(cattle)
Definition	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223620.4

ORF Insert Sequence:

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCT CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCG GCAGGTGAGG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCG AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAA GGCACATGTG GCCCATCACC CCAGCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAACGC 
AATGGGGAGG ACCAGATCCA GGACATGGAG CTTGTGGAAA CCAGGCCTTC AGGGGACGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGGGCCC CTCACCCTGA GATGGGGTGA AAAAGGACGG 
ATCTACACCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCACGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA-NC1 ( XM_005223620.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223677.1 Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa32123
Clone ID Related Accession (Same CDS sequence)	XM_005223620.4 , XM_005223620.3
Accession Version	XM_005223620.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	non-classical MHC class I antigen isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCT CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCG GCAGGTGAGG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCG AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAA GGCACATGTG GCCCATCACC CCAGCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAACGC 
AATGGGGAGG ACCAGATCCA GGACATGGAG CTTGTGGAAA CCAGGCCTTC AGGGGACGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGGGCCC CTCACCCTGA GATGGGGTGA AAAAGGACGG 
ATCTACACCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCACGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223677.1
CDS	66..1037
Translation	MGPRTLLLLLLGVLVPRDTRAGPHSMRYFLTAVSRPGLGEPRFITVGYVDDTQFVRFDSDRPDPRMEPRARWVEDEGPEYWDQETRIQKENTQTFRANLNTLLGYYNQSEAGSHTIQWMHGCGVGSDGRLLRGYNQLAYDGKDYLALNEDLRSWTAADTAAQITKRKWEAAGEAERFRNYLEGECVKLLRRHLENGKDTLLRADPPKAHVAHHPSSEREVTLRCWALGFYPEEISLTWQRNGEDQIQDMELVETRPSGDGTFQKWAALAVPSGEEQRYTCRVQHEGLQGPLTLRWGEKGRIYTQASSSDSAQGSDVSLTVPKV

Target ORF information:

RefSeq Version	XM_005223620.3
Organism	Bos taurus(cattle)
Definition	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223620.3

ORF Insert Sequence:

ATGGGGCCGC GAACCCTCCT CCTGCTCCTC TTGGGGGTCC TGGTCCCCCG AGACACACGG 
GCGGGCCCTC ACTCCATGAG GTATTTCCTC ACCGCCGTGT CCCGGCCCGG CCTCGGGGAG 
CCCCGCTTCA TCACCGTCGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC 
CGTCCAGATC CGAGGATGGA GCCGCGGGCG CGGTGGGTGG AGGATGAGGG GCCGGAGTAT 
TGGGATCAAG AGACGCGGAT ACAAAAGGAA AACACACAGA CTTTCCGAGC AAACCTGAAC 
ACCCTACTCG GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACACCATCCA GTGGATGCAC 
GGCTGCGGCG TGGGGTCGGA CGGGCGCCTC CTCCGCGGGT ATAATCAGCT CGCCTATGAC 
GGCAAAGATT ACCTCGCCCT GAACGAGGAC CTGCGCTCCT GGACCGCGGC GGACACAGCG 
GCTCAGATCA CCAAGCGCAA GTGGGAGGCG GCAGGTGAGG CGGAGAGATT CAGGAACTAC 
CTGGAGGGCG AGTGCGTGAA GTTGCTCCGC AGACACCTGG AGAACGGGAA GGACACGCTG 
CTGCGCGCAG ACCCTCCAAA GGCACATGTG GCCCATCACC CCAGCTCTGA GCGTGAGGTC 
ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGAGGAGA TCTCACTGAC CTGGCAACGC 
AATGGGGAGG ACCAGATCCA GGACATGGAG CTTGTGGAAA CCAGGCCTTC AGGGGACGGA 
ACCTTCCAGA AGTGGGCGGC CCTGGCGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC 
CGTGTGCAGC ACGAGGGGCT TCAGGGGCCC CTCACCCTGA GATGGGGTGA AAAAGGACGG 
ATCTACACCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCACGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA-NC1 ( XM_024983518.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839286.1 Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229456
Clone ID Related Accession (Same CDS sequence)	XM_024983518.1
Accession Version	XM_024983518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	non-classical MHC class I antigen isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTATT TCCTCACCGC CGTGTCCCGG CCCGGCCTCG GGGAGCCCCG CTTCATCACC 
GTCGGCTACG TGGACGACAC GCAGTTCGTG CGGTTCGACA GCGACCGTCC AGATCCGAGG 
ATGGAGCCGC GGGCGCGGTG GGTGGAGGAT GAGGGGCCGG AGTATTGGGA TCAAGAGACG 
CGGATACAAA AGGAAAACAC ACAGACTTTC CGAGCAAACC TGAACACCCT ACTCGGCTAC 
TACAACCAGA GCGAGGCCGG GTCTCACACC ATCCAGTGGA TGCACGGCTG CGGCGTGGGG 
TCGGACGGGC GCCTCCTCCG CGGGTATAAT CAGCTCGCCT ATGACGGCAA AGATTACCTC 
GCCCTGAACG AGGACCTGCG CTCCTGGACC GCGGCGGACA CAGCGGCTCA GATCACCAAG 
CGCAAGTGGG AGGCGGCAGG TGAGGCGGAG AGATTCAGGA ACTACCTGGA GGGCGAGTGC 
GTGAAGTTGC TCCGCAGACA CCTGGAGAAC GGGAAGGACA CGCTGCTGCG CGCAGACCCT 
CCAAAGGCAC ATGTGGCCCA TCACCCCAGC TCTGAGCGTG AGGTCACCCT GAGGTGCTGG 
GCCCTGGGCT TCTACCCTGA GGAGATCTCA CTGACCTGGC AACGCAATGG GGAGGACCAG 
ATCCAGGACA TGGAGCTTGT GGAAACCAGG CCTTCAGGGG ACGGAACCTT CCAGAAGTGG 
GCGGCCCTGG CGGTGCCTTC TGGAGAGGAG CAGAGATACA CGTGCCGTGT GCAGCACGAG 
GGGCTTCAGG GGCCCCTCAC CCTGAGATGG GGTGAAAAAG GACGGATCTA CACCCAGGCT 
TCAAGCAGTG ACAGTGCCCA GGGCTCTGAT GTGTCTCTCA CGGTTCCTAA AGAGTCCTGG 
TCTCATTCTT CTCTGGGTGA TCCCATCTCT GACAGCAGCA GGGGTCACTT TGCCTGTCAT 
TGTCCCCACA AGTCAAGGGA TCCATACACA CAGGATCTCA GCGGTGTCAA GAGGGAAATT 
TCAGAGCCAT CCAGCTCTTG CCATCCTAGA AATTTTGCCG GATTCTTCTT CCAACTTTTC 
TCCATCTTTA AAAAGAACCA CGTGATGGAA CTTGCAAAGA AGAGGGACCC GATAGTTTTC 
TTATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839286.1
CDS	287..1435
Translation	MRYFLTAVSRPGLGEPRFITVGYVDDTQFVRFDSDRPDPRMEPRARWVEDEGPEYWDQETRIQKENTQTFRANLNTLLGYYNQSEAGSHTIQWMHGCGVGSDGRLLRGYNQLAYDGKDYLALNEDLRSWTAADTAAQITKRKWEAAGEAERFRNYLEGECVKLLRRHLENGKDTLLRADPPKAHVAHHPSSEREVTLRCWALGFYPEEISLTWQRNGEDQIQDMELVETRPSGDGTFQKWAALAVPSGEEQRYTCRVQHEGLQGPLTLRWGEKGRIYTQASSSDSAQGSDVSLTVPKESWSHSSLGDPISDSSRGHFACHCPHKSRDPYTQDLSGVKREISEPSSSCHPRNFAGFFFQLFSIFKKNHVMELAKKRDPIVFLS

Target ORF information:

RefSeq Version	XM_024983518.1
Organism	Bos taurus(cattle)
Definition	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983518.1

ORF Insert Sequence:

ATGAGGTATT TCCTCACCGC CGTGTCCCGG CCCGGCCTCG GGGAGCCCCG CTTCATCACC 
GTCGGCTACG TGGACGACAC GCAGTTCGTG CGGTTCGACA GCGACCGTCC AGATCCGAGG 
ATGGAGCCGC GGGCGCGGTG GGTGGAGGAT GAGGGGCCGG AGTATTGGGA TCAAGAGACG 
CGGATACAAA AGGAAAACAC ACAGACTTTC CGAGCAAACC TGAACACCCT ACTCGGCTAC 
TACAACCAGA GCGAGGCCGG GTCTCACACC ATCCAGTGGA TGCACGGCTG CGGCGTGGGG 
TCGGACGGGC GCCTCCTCCG CGGGTATAAT CAGCTCGCCT ATGACGGCAA AGATTACCTC 
GCCCTGAACG AGGACCTGCG CTCCTGGACC GCGGCGGACA CAGCGGCTCA GATCACCAAG 
CGCAAGTGGG AGGCGGCAGG TGAGGCGGAG AGATTCAGGA ACTACCTGGA GGGCGAGTGC 
GTGAAGTTGC TCCGCAGACA CCTGGAGAAC GGGAAGGACA CGCTGCTGCG CGCAGACCCT 
CCAAAGGCAC ATGTGGCCCA TCACCCCAGC TCTGAGCGTG AGGTCACCCT GAGGTGCTGG 
GCCCTGGGCT TCTACCCTGA GGAGATCTCA CTGACCTGGC AACGCAATGG GGAGGACCAG 
ATCCAGGACA TGGAGCTTGT GGAAACCAGG CCTTCAGGGG ACGGAACCTT CCAGAAGTGG 
GCGGCCCTGG CGGTGCCTTC TGGAGAGGAG CAGAGATACA CGTGCCGTGT GCAGCACGAG 
GGGCTTCAGG GGCCCCTCAC CCTGAGATGG GGTGAAAAAG GACGGATCTA CACCCAGGCT 
TCAAGCAGTG ACAGTGCCCA GGGCTCTGAT GTGTCTCTCA CGGTTCCTAA AGAGTCCTGG 
TCTCATTCTT CTCTGGGTGA TCCCATCTCT GACAGCAGCA GGGGTCACTT TGCCTGTCAT 
TGTCCCCACA AGTCAAGGGA TCCATACACA CAGGATCTCA GCGGTGTCAA GAGGGAAATT 
TCAGAGCCAT CCAGCTCTTG CCATCCTAGA AATTTTGCCG GATTCTTCTT CCAACTTTTC 
TCCATCTTTA AAAAGAACCA CGTGATGGAA CTTGCAAAGA AGAGGGACCC GATAGTTTTC 
TTATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA-NC1 ( XM_024983519.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839287.1 Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa229457
Clone ID Related Accession (Same CDS sequence)	XM_024983519.1
Accession Version	XM_024983519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	non-classical MHC class I antigen isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTATT TCCTCACCGC CGTGTCCCGG CCCGGCCTCG GGGAGCCCCG CTTCATCACC 
GTCGGCTACG TGGACGACAC GCAGTTCGTG CGGTTCGACA GCGACCGTCC AGATCCGAGG 
ATGGAGCCGC GGGCGCGGTG GGTGGAGGAT GAGGGGCCGG AGTATTGGGA TCAAGAGACG 
CGGATACAAA AGGAAAACAC ACAGACTTTC CGAGCAAACC TGAACACCCT ACTCGGCTAC 
TACAACCAGA GCGAGGCCGG GTCTCACACC ATCCAGTGGA TGCACGGCTG CGGCGTGGGG 
TCGGACGGGC GCCTCCTCCG CGGGTATAAT CAGCTCGCCT ATGACGGCAA AGATTACCTC 
GCCCTGAACG AGGACCTGCG CTCCTGGACC GCGGCGGACA CAGCGGCTCA GATCACCAAG 
CGCAAGTGGG AGGCGGCAGG TGAGGCGGAG AGATTCAGGA ACTACCTGGA GGGCGAGTGC 
GTGAAGTTGC TCCGCAGACA CCTGGAGAAC GGGAAGGACA CGCTGCTGCG CGCAGACCCT 
CCAAAGGCAC ATGTGGCCCA TCACCCCAGC TCTGAGCGTG AGGTCACCCT GAGGTGCTGG 
GCCCTGGGCT TCTACCCTGA GGAGATCTCA CTGACCTGGC AACGCAATGG GGAGGACCAG 
ATCCAGGACA TGGAGCTTGT GGAAACCAGG CCTTCAGGGG ACGGAACCTT CCAGAAGTGG 
GCGGCCCTGG CGGTGCCTTC TGGAGAGGAG CAGAGATACA CGTGCCGTGT GCAGCACGAG 
GGGCTTCAGG GGCCCCTCAC CCTGAGATGG GGTGAAAAAG GACGGATCTA CACCCAGGCT 
TCAAGCAGTG ACAGTGCCCA GGGCTCTGAT GTGTCTCTCA CGGTTCCTAA AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839287.1
CDS	285..1181
Translation	MRYFLTAVSRPGLGEPRFITVGYVDDTQFVRFDSDRPDPRMEPRARWVEDEGPEYWDQETRIQKENTQTFRANLNTLLGYYNQSEAGSHTIQWMHGCGVGSDGRLLRGYNQLAYDGKDYLALNEDLRSWTAADTAAQITKRKWEAAGEAERFRNYLEGECVKLLRRHLENGKDTLLRADPPKAHVAHHPSSEREVTLRCWALGFYPEEISLTWQRNGEDQIQDMELVETRPSGDGTFQKWAALAVPSGEEQRYTCRVQHEGLQGPLTLRWGEKGRIYTQASSSDSAQGSDVSLTVPKV

Target ORF information:

RefSeq Version	XM_024983519.1
Organism	Bos taurus(cattle)
Definition	Bos taurus non-classical MHC class I antigen (BOLA-NC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983519.1

ORF Insert Sequence:

ATGAGGTATT TCCTCACCGC CGTGTCCCGG CCCGGCCTCG GGGAGCCCCG CTTCATCACC 
GTCGGCTACG TGGACGACAC GCAGTTCGTG CGGTTCGACA GCGACCGTCC AGATCCGAGG 
ATGGAGCCGC GGGCGCGGTG GGTGGAGGAT GAGGGGCCGG AGTATTGGGA TCAAGAGACG 
CGGATACAAA AGGAAAACAC ACAGACTTTC CGAGCAAACC TGAACACCCT ACTCGGCTAC 
TACAACCAGA GCGAGGCCGG GTCTCACACC ATCCAGTGGA TGCACGGCTG CGGCGTGGGG 
TCGGACGGGC GCCTCCTCCG CGGGTATAAT CAGCTCGCCT ATGACGGCAA AGATTACCTC 
GCCCTGAACG AGGACCTGCG CTCCTGGACC GCGGCGGACA CAGCGGCTCA GATCACCAAG 
CGCAAGTGGG AGGCGGCAGG TGAGGCGGAG AGATTCAGGA ACTACCTGGA GGGCGAGTGC 
GTGAAGTTGC TCCGCAGACA CCTGGAGAAC GGGAAGGACA CGCTGCTGCG CGCAGACCCT 
CCAAAGGCAC ATGTGGCCCA TCACCCCAGC TCTGAGCGTG AGGTCACCCT GAGGTGCTGG 
GCCCTGGGCT TCTACCCTGA GGAGATCTCA CTGACCTGGC AACGCAATGG GGAGGACCAG 
ATCCAGGACA TGGAGCTTGT GGAAACCAGG CCTTCAGGGG ACGGAACCTT CCAGAAGTGG 
GCGGCCCTGG CGGTGCCTTC TGGAGAGGAG CAGAGATACA CGTGCCGTGT GCAGCACGAG 
GGGCTTCAGG GGCCCCTCAC CCTGAGATGG GGTGAAAAAG GACGGATCTA CACCCAGGCT 
TCAAGCAGTG ACAGTGCCCA GGGCTCTGAT GTGTCTCTCA CGGTTCCTAA AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel RNAseq-assisted method for MHC class I genotyping in a non-model species applied to a lethal vaccination-induced alloimmune disease.
BMC genomics17365(2016 05)
Demasius W,Weikard R,Hadlich F,Buitkamp J,K?hn C

Non-classical major histocompatibility complex class makes a crucial contribution to reproduction in the dairy cow.
The Journal of reproduction and development58(5)569-75(2012)
Shu L,Peng X,Zhang S,Deng G,Wu Y,He M,Li B,Li C,Zhang K

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

BoLA class I allele diversity and polymorphism in a herd of cattle.
Immunogenetics59(2)167-76(2007 Feb)
Babiuk S,Horseman B,Zhang C,Bickis M,Kusalik A,Schook LB,Abrahamsen MS,Pontarollo R

Evidence for expression of both classical and non-classical major histocompatibility complex class I genes in bovine trophoblast cells.
American journal of reproductive immunology (New York, N.Y. : 1989)55(3)188-200(2006 Mar)
Davies CJ,Eldridge JA,Fisher PJ,Schlafer DH

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BOLA-NC1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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