ALPK2 cDNA ORF clone, Bos taurus(cattle)

The following ALPK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa229865 XM_024984220.1
Latest version!
Bos taurus alpha kinase 2 (ALPK2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa47602 XM_010818825.3
Latest version!
Bos taurus alpha kinase 2 (ALPK2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa229863 XM_024984218.1
Latest version!
Bos taurus alpha kinase 2 (ALPK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa229864 XM_024984219.1
Latest version!
Bos taurus alpha kinase 2 (ALPK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa88312 XM_015460089.2
Latest version!
Bos taurus alpha kinase 2 (ALPK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa88313 XM_015460090.2
Latest version!
Bos taurus alpha kinase 2 (ALPK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa88314 XM_015460091.2
Latest version!
Bos taurus alpha kinase 2 (ALPK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa39620 NM_001192248.1
Latest version!
Bos taurus alpha kinase 2 (ALPK2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa32523 XM_005224149.4
Latest version!
Bos taurus alpha kinase 2 (ALPK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa32523 XM_005224149.3 Bos taurus alpha kinase 2 (ALPK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa88313 XM_015460090.1 Bos taurus alpha kinase 2 (ALPK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa88314 XM_015460091.1 Bos taurus alpha kinase 2 (ALPK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa47602 XM_010818825.2 Bos taurus alpha kinase 2 (ALPK2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa88312 XM_015460089.1 Bos taurus alpha kinase 2 (ALPK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa229865
    Clone ID Related Accession (Same CDS sequence) XM_024984220.1
    Accession Version XM_024984220.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG GAGACAACTC
    CATGGTGAGC TTGGCCATCC TTCAAGCCAG TCAGAAGGAC CAGGGCCTCT ATTACTGCTG
    CATCAAGAAC AGTTACGGGA GAGTGACCGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839988.1
    CDS653..6205
    Translation

    Target ORF information:

    RefSeq Version XM_024984220.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984220.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG GAGACAACTC
    CATGGTGAGC TTGGCCATCC TTCAAGCCAG TCAGAAGGAC CAGGGCCTCT ATTACTGCTG
    CATCAAGAAC AGTTACGGGA GAGTGACCGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47602
    Clone ID Related Accession (Same CDS sequence) XM_010818825.3 , XM_010818825.2
    Accession Version XM_010818825.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGCTCC AGTATTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817127.1
    CDS655..5865
    Translation

    Target ORF information:

    RefSeq Version XM_010818825.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818825.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGCTCC AGTATTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47602
    Clone ID Related Accession (Same CDS sequence) XM_010818825.3 , XM_010818825.2
    Accession Version XM_010818825.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGCTCC AGTATTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817127.1
    CDS93..5303
    Translation

    Target ORF information:

    RefSeq Version XM_010818825.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818825.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGCTCC AGTATTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229863
    Clone ID Related Accession (Same CDS sequence) XM_024984218.1
    Accession Version XM_024984218.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGTA
    TATGAAGAGA TTGAATTCAG CCAGCTCATC TTCCGAGAGG ACTTCCTCCG TGACAGCTAC
    TTCGGGGGCC GCCTGCATGG CCAGATCGCC ACGGAGGAGC TGCACTTCGG GGAAGGCGTC
    CACCGGAGAG CCTTCCGCAG CAAGGTGCTG CGCGGCCTGA CGCCGGTCTT CAAGCCCGGC
    CACGCCTGCG TGCTCAAGGT GCACAACGCC GTCGCCTACG GGGCCAGAAA CAACGAGGAG
    CTCATCCAGA GGAACTACAG ACTCGCCGCG CAGGAATGCT ACGTGCAGAA CACTGCCAGG
    CACTACGCGC AGATCTACGC CGCAGAAGCC CAGCCTCTGG AAGGCTTCGG GGAAGTGCCC
    GAGATCATTC CTATTTTCCT TATCCATCGA CCTGAGAATA ACATCCCGTA CGCAACCGTG
    GAGGAAGAGC TGATTGGAGA ATTCGTGAAG TATTCCATCA GGGATGGGAA GGAGATAAAC
    TTCTTGAGGA GAGAGTCGGA GGCTGGGCAG AAGTGTTGCA CCTTCCAGCA CTGGGTCTAC
    CAGAAAACAA GTGGCTGCCT GCTGGTCACG GACATGCAGG GTGTAGGCAT GAAGTTAACT
    GATGTTGGCA TAGCAACAGA GGCTACAGGG TACAAAGGAT TTAAAGGCAA CTGTTCCATG
    ACCTTCATCG ATCAGTTTAA AGCCCTTCAC CAGTGTAATA AGTACTGTAA AATGCTGGGG
    TTGAAGTCGC TTCAGGACAA CAGCCAGAAG CAGAAGAAGC CAAGCATCGG GCGGAGCAAG
    ATTCAGCCCG GCACCACAGC CGTGAAGAAG GCGGCCTCCG GGAGCCTGGC AGAAAAGAAA
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839986.1
    CDS649..7074
    Translation

    Target ORF information:

    RefSeq Version XM_024984218.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984218.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGTA
    TATGAAGAGA TTGAATTCAG CCAGCTCATC TTCCGAGAGG ACTTCCTCCG TGACAGCTAC
    TTCGGGGGCC GCCTGCATGG CCAGATCGCC ACGGAGGAGC TGCACTTCGG GGAAGGCGTC
    CACCGGAGAG CCTTCCGCAG CAAGGTGCTG CGCGGCCTGA CGCCGGTCTT CAAGCCCGGC
    CACGCCTGCG TGCTCAAGGT GCACAACGCC GTCGCCTACG GGGCCAGAAA CAACGAGGAG
    CTCATCCAGA GGAACTACAG ACTCGCCGCG CAGGAATGCT ACGTGCAGAA CACTGCCAGG
    CACTACGCGC AGATCTACGC CGCAGAAGCC CAGCCTCTGG AAGGCTTCGG GGAAGTGCCC
    GAGATCATTC CTATTTTCCT TATCCATCGA CCTGAGAATA ACATCCCGTA CGCAACCGTG
    GAGGAAGAGC TGATTGGAGA ATTCGTGAAG TATTCCATCA GGGATGGGAA GGAGATAAAC
    TTCTTGAGGA GAGAGTCGGA GGCTGGGCAG AAGTGTTGCA CCTTCCAGCA CTGGGTCTAC
    CAGAAAACAA GTGGCTGCCT GCTGGTCACG GACATGCAGG GTGTAGGCAT GAAGTTAACT
    GATGTTGGCA TAGCAACAGA GGCTACAGGG TACAAAGGAT TTAAAGGCAA CTGTTCCATG
    ACCTTCATCG ATCAGTTTAA AGCCCTTCAC CAGTGTAATA AGTACTGTAA AATGCTGGGG
    TTGAAGTCGC TTCAGGACAA CAGCCAGAAG CAGAAGAAGC CAAGCATCGG GCGGAGCAAG
    ATTCAGCCCG GCACCACAGC CGTGAAGAAG GCGGCCTCCG GGAGCCTGGC AGAAAAGAAA
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229864
    Clone ID Related Accession (Same CDS sequence) XM_024984219.1
    Accession Version XM_024984219.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGATG ACAACGCTTT AGGAAAAACC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839987.1
    CDS650..6892
    Translation

    Target ORF information:

    RefSeq Version XM_024984219.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGATG ACAACGCTTT AGGAAAAACC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88312
    Clone ID Related Accession (Same CDS sequence) XM_015460089.2 , XM_015460089.1
    Accession Version XM_015460089.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGGTG TAGGCATGAA GTTAACTGAT
    GTTGGCATAG CAACAGAGGC TACAGGGTAC AAAGGATTTA AAGGCAACTG TTCCATGACC
    TTCATCGATC AGTTTAAAGC CCTTCACCAG TGTAATAAGT ACTGTAAAAT GCTGGGGTTG
    AAGTCGCTTC AGGACAACAG CCAGAAGCAG AAGAAGCCAA GCATCGGGCG GAGCAAGATT
    CAGCCCGGCA CCACAGCCGT GAAGAAGGCG GCCTCCGGGA GCCTGGCAGA AAAGAAAAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315575.1
    CDS649..7131
    Translation

    Target ORF information:

    RefSeq Version XM_015460089.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460089.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGGTG TAGGCATGAA GTTAACTGAT
    GTTGGCATAG CAACAGAGGC TACAGGGTAC AAAGGATTTA AAGGCAACTG TTCCATGACC
    TTCATCGATC AGTTTAAAGC CCTTCACCAG TGTAATAAGT ACTGTAAAAT GCTGGGGTTG
    AAGTCGCTTC AGGACAACAG CCAGAAGCAG AAGAAGCCAA GCATCGGGCG GAGCAAGATT
    CAGCCCGGCA CCACAGCCGT GAAGAAGGCG GCCTCCGGGA GCCTGGCAGA AAAGAAAAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88312
    Clone ID Related Accession (Same CDS sequence) XM_015460089.2 , XM_015460089.1
    Accession Version XM_015460089.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGGTG TAGGCATGAA GTTAACTGAT
    GTTGGCATAG CAACAGAGGC TACAGGGTAC AAAGGATTTA AAGGCAACTG TTCCATGACC
    TTCATCGATC AGTTTAAAGC CCTTCACCAG TGTAATAAGT ACTGTAAAAT GCTGGGGTTG
    AAGTCGCTTC AGGACAACAG CCAGAAGCAG AAGAAGCCAA GCATCGGGCG GAGCAAGATT
    CAGCCCGGCA CCACAGCCGT GAAGAAGGCG GCCTCCGGGA GCCTGGCAGA AAAGAAAAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315575.1
    CDS93..6575
    Translation

    Target ORF information:

    RefSeq Version XM_015460089.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGGTG TAGGCATGAA GTTAACTGAT
    GTTGGCATAG CAACAGAGGC TACAGGGTAC AAAGGATTTA AAGGCAACTG TTCCATGACC
    TTCATCGATC AGTTTAAAGC CCTTCACCAG TGTAATAAGT ACTGTAAAAT GCTGGGGTTG
    AAGTCGCTTC AGGACAACAG CCAGAAGCAG AAGAAGCCAA GCATCGGGCG GAGCAAGATT
    CAGCCCGGCA CCACAGCCGT GAAGAAGGCG GCCTCCGGGA GCCTGGCAGA AAAGAAAAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88313
    Clone ID Related Accession (Same CDS sequence) XM_015460090.1 , XM_015460090.2
    Accession Version XM_015460090.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGGTG TAGGCATGAA GTTAACTGAT
    GTTGGCATAG CAACAGAGGC TACAGGGAAC CAATGGCATC AGTGGGAGCT CAGAGCTGCA
    TTAATAGATA CACGGGATTT CGGACCCCAC CCCAGGCCTA CTGAATCCAA ACCAGCACGT
    GAACACGGCC CTCGAGTGAT CTTTATGCCC CACTTCCGTC CAAGGGTCAA GATATTTAAG
    AAAAGACTCT ATCGGATGGT GTTCAGCAGC ATAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315576.1
    CDS93..6551
    Translation

    Target ORF information:

    RefSeq Version XM_015460090.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGGTG TAGGCATGAA GTTAACTGAT
    GTTGGCATAG CAACAGAGGC TACAGGGAAC CAATGGCATC AGTGGGAGCT CAGAGCTGCA
    TTAATAGATA CACGGGATTT CGGACCCCAC CCCAGGCCTA CTGAATCCAA ACCAGCACGT
    GAACACGGCC CTCGAGTGAT CTTTATGCCC CACTTCCGTC CAAGGGTCAA GATATTTAAG
    AAAAGACTCT ATCGGATGGT GTTCAGCAGC ATAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88313
    Clone ID Related Accession (Same CDS sequence) XM_015460090.1 , XM_015460090.2
    Accession Version XM_015460090.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGGTG TAGGCATGAA GTTAACTGAT
    GTTGGCATAG CAACAGAGGC TACAGGGAAC CAATGGCATC AGTGGGAGCT CAGAGCTGCA
    TTAATAGATA CACGGGATTT CGGACCCCAC CCCAGGCCTA CTGAATCCAA ACCAGCACGT
    GAACACGGCC CTCGAGTGAT CTTTATGCCC CACTTCCGTC CAAGGGTCAA GATATTTAAG
    AAAAGACTCT ATCGGATGGT GTTCAGCAGC ATAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315576.1
    CDS650..7108
    Translation

    Target ORF information:

    RefSeq Version XM_015460090.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460090.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAG
    ATCATTCCTA TTTTCCTTAT CCATCGACCT GAGAATAACA TCCCGTACGC AACCGTGGAG
    GAAGAGCTGA TTGGAGAATT CGTGAAGTAT TCCATCAGGG ATGGGAAGGA GATAAACTTC
    TTGAGGAGAG AGTCGGAGGC TGGGCAGAAG TGTTGCACCT TCCAGCACTG GGTCTACCAG
    AAAACAAGTG GCTGCCTGCT GGTCACGGAC ATGCAGGGTG TAGGCATGAA GTTAACTGAT
    GTTGGCATAG CAACAGAGGC TACAGGGAAC CAATGGCATC AGTGGGAGCT CAGAGCTGCA
    TTAATAGATA CACGGGATTT CGGACCCCAC CCCAGGCCTA CTGAATCCAA ACCAGCACGT
    GAACACGGCC CTCGAGTGAT CTTTATGCCC CACTTCCGTC CAAGGGTCAA GATATTTAAG
    AAAAGACTCT ATCGGATGGT GTTCAGCAGC ATAAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88314
    Clone ID Related Accession (Same CDS sequence) XM_015460091.2 , XM_015460091.1
    Accession Version XM_015460091.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAA
    ACAGCAGATC GCGAAAAACT CAGTCCTATG ATACAACAAG TAAAGACGGT GGTGCATCAG
    GGAAAAGCAA ATTGGAAAAT CAGGGCCGTG GGAGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315577.1
    CDS651..6749
    Translation

    Target ORF information:

    RefSeq Version XM_015460091.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460091.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAA
    ACAGCAGATC GCGAAAAACT CAGTCCTATG ATACAACAAG TAAAGACGGT GGTGCATCAG
    GGAAAAGCAA ATTGGAAAAT CAGGGCCGTG GGAGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88314
    Clone ID Related Accession (Same CDS sequence) XM_015460091.2 , XM_015460091.1
    Accession Version XM_015460091.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAA
    ACAGCAGATC GCGAAAAACT CAGTCCTATG ATACAACAAG TAAAGACGGT GGTGCATCAG
    GGAAAAGCAA ATTGGAAAAT CAGGGCCGTG GGAGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315577.1
    CDS93..6191
    Translation

    Target ORF information:

    RefSeq Version XM_015460091.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGGC
    TTTCTCTTCT GTCTTCTAGT TCTCAAACAG CTCTCAAGTC ACCCAGATGT TAAAGTATAT
    GAAGAGATTG AATTCAGCCA GCTCATCTTC CGAGAGGACT TCCTCCGTGA CAGCTACTTC
    GGGGGCCGCC TGCATGGCCA GATCGCCACG GAGGAGCTGC ACTTCGGGGA AGGCGTCCAC
    CGGAGAGCCT TCCGCAGCAA GGTGCTGCGC GGCCTGACGC CGGTCTTCAA GCCCGGCCAC
    GCCTGCGTGC TCAAGGTGCA CAACGCCGTC GCCTACGGGG CCAGAAACAA CGAGGAGCTC
    ATCCAGAGGA ACTACAGACT CGCCGCGCAG GAATGCTACG TGCAGAACAC TGCCAGGCAC
    TACGCGCAGA TCTACGCCGC AGAAGCCCAG CCTCTGGAAG GCTTCGGGGA AGTGCCCGAA
    ACAGCAGATC GCGAAAAACT CAGTCCTATG ATACAACAAG TAAAGACGGT GGTGCATCAG
    GGAAAAGCAA ATTGGAAAAT CAGGGCCGTG GGAGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39620
    Clone ID Related Accession (Same CDS sequence) NM_001192248.1
    Accession Version NM_001192248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000024.1. On Jul 17, 2010 this sequence version replaced XM_587341.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGTTTCAA GGACAAGCCA GCACTTAACA GTGGAGTTCG AAACTGGCCA GCCCAAGCCA 
    GAGGTAACTT GGTATAAGAA TGGTCGAACC ATAGATGAAT GTGGCAGTGT CTCCAGCTAC
    GAATTCTTCG AGAATCAGTA TGTTCACGTA TTACACCTCT ATTGCTGTAC CCAAAATGAC
    ACTGCTGTTT ATCAAGTCTC TGCTCGGAAC TGTTTTGGGA TGATCTGCTG CTCTGCTTCC
    ATTGAAGTGC AGTGCCCGAC AGAGGACCAA CCACTCTCTC CCAACCCAAA AGATGACGGG
    CACACAGGTT GGAAACACGA CACGGAGACA TATGAGCAGG AGAGCCCAAA CCACACTGAT
    GAGAAAGAGC ATCCTTATAA GGAAGGAGAA GGTATCGCCT CGGGGCCTCC TACATCAGCT
    GACGCCCCCT CCTCCAAATC CAGCGGTGCT CATTCACTCC AGGTATCGGC CGACCGTGAC
    CCTGGCGCGT CTGGCTCTGA CAACCCCTTG GCGGTTAAAG ACACGAGGCA AACGGAAGAG
    GCTGGTGGTG CTGCTAACAC AGAGGGCGTT GCGGACGGGT TGCGTTTTCC TAGTTCAAGT
    GATGCTCCTG ATAAACAAGA TGTGTGTGGC CACAGGGCCG TGCATTCCGA AGTACCAAGG
    TTAACAGATG GTGCCTTAGA CCCTGAGGGC CCTAACGAGG AAGCCTTGAA CTCTAGTCAC
    CAAAATGCCA GAGTCCAGAA GTATATCAGC TTCGGCCCGG CCCTGACACG GGCAGACTCG
    TCCGCTCCTC CAGGTACCAG GCCCGTCAGC CCCCCGGCTT CCAGCACAGA CTCTGACAGT
    GACTATGAAC TTTGCCCAGA GATAACCCTA ACCTGCGCCG AGGAGTTTTC AGATGATGAC
    CTGGAGTATC TGGAATGTTC CGACGTTATG ACAGATTACT CTAATGCGAT CTGGCAAAGG
    AACCTGCAGG GGACGGAGCG TGTTTTTTTA TTAGAAAGCG ATGACGAAGA GATGGAATTC
    AGTGAGTGTA GCCTGGGTGG GTGTGCGCGT TTCTTCAGTG AATTGGGTTG CAGGCCCCCG
    GTGTCAGATG ACACGGGCCC CATGGATGCC ACCGCTGGCC TCTGTGGTCA TCACTCACCA
    CCCCAAGAAG TGGGGGGAAG GAGCAGCCGG GCCTCCACGC ACAGAGCCTC ATCCTTGCAA
    GAGGGGATGC CCCTGCCCCT GGGACTTCAG CAGGATGGAC CGGCTATGGT GACAGACCTG
    GGCAGGTATA AACCACCCGC TGCCTTGGAG GCTGCTGAAG ACGGTTATCC AGGAATTCAA
    GGAGAAACCA GAGACAGCCA CCAGGCCGGA AAGGAATTCT CTGGTGACAA GCTGCTAAAC
    ATGGATAAAG CGGTGGCAGG GACAGAGGGG AAGCACTTGT CTGGTGAGTC AGGACAGCCA
    GGGATGAGCC GGTGTCTGGA GACCAGGGCT GAGAGAAGAG TAGGGGGAAA AGACGGATGG
    AGCCAGCGGG GCTCTGAGAA ACCTGCCAGG ACGTGGCGGC CGGGAATTAA GGGAAAAGCC
    AAGAGGCTGA ATTCCAGCCT GGAAGAAAGG ACGGCGGAAG CCTCCCTGGA TCGCCTCTGT
    CCCAAGGGAC CTGTTAAGCA CCCACTAACC CCGAGTGACA AAAGAGTCAG CTCCCATGCC
    AGAGCAGAGG GGGCTGATCT GAAGCCCCAG TTGCCTGCAG GAGGCCCTGA GGTTCCAACC
    CAAGCAGAGC CAGAAGTGAA AACCCTGCAG GCTCCACCCG GCTTGCTCTC CAAGGAGGAG
    ACCTTGCACT TCCAGAGAGA AGGGGTACGG GTTACCGACG TATTTGAAAC GTGCAAGGTT
    TCAGGCTGGA GTGATCATCC TCAGGTACAA ATTCAGGAAA CAGTCAGGGA GCAAATCTCT
    CTCAGCCACA TGCCGGCTTT CTCGGAGCCT ACTGGGGAGG AGCCTACTTT TCCTGGGACC
    ACAACAGAGT CCCTCCCCAA CTTAGGAGGG ATCGACAAAG AAAGTGCATC ATTGGCCAAA
    CACCCGGAGG TAGACGGTTG TACCCAAGGG GCACGGCATG AAGAAAACCA AGGTGACAAC
    ACGCTCAGAC GTTCTGGAAG AGACCTGGGG CGTGTGCTGA GCATCCCAGA AGCCAGCAGT
    GAAGCCACGT CCCCCTGTGA GCTGTTGGGG CCTCCCCCCC AGCCCCAGGC TGCCTCTGCG
    TCCCCTGAAT TTGCACATAC TGTCCCCGCC CTGGAAACCA TGTGTGCTGC TGGGCCAGGG
    GACGGAGTAG CTGTGTGTGG CCCGGAGCGT CTTGAAGCAG GTGACCAGGA AGCATGTGAT
    ATTGTGGGCT CTCCGGTTGG AGCCCCAGTT GGTAAACATT TGCCTCAAGA AATGTGCTCC
    CTGGACTTAG AGCTGGCAGG TCAAAGCAAA CCACCTGATT TATGTCCTCC CGATGACAAG
    ACTCTGGATG TTCTCTCTCA GACACAAGGT CCTGAGCCTC CCCAGACTAC GTGCGGGAGC
    AGTGGCGAGA GAACCTCTCC CGACTCCCCT CTTTTCATCA GCACTTTCAC CTGGAACATC
    TCACAAAAAG CCAGTAAAGG TGCCACCGGG GAGGATCTAG CCAACATAGA GGGTTCCGCC
    TCCACGTCGG CCTCCATAGT AAAGGCTGGC CAGGAGAGGC TGAGCCCCAG CCACTCAGGG
    GACCTTGAGG AAAGATGGCC TCCATCTCCA GAGAGCAGCT CTTCCGTTTG GGCCATAGAG
    GGCAGTGACA AGAACTCCAG CCCCGGTGCC CCGGACTCGG CAGACATACC GGCAGGTCAT
    AGTCCAGTAA CGAAGTTTCC TCGGGAGAAG CCCACAACAC TAATTGCTGA CGTTGAGTGT
    TCTGAGGTGA CTGGGGCGAA TAGCAACACA TCTGCTCTTT CCGTTGCCCC GAAAGGCCAC
    CCATCCAAAT ACCTCGCTGT TTCAGTTGCT GGGGACAGCC ATGCAGGTGG CCCTGAGGAG
    AGTTCACCTC GGGCACCAGA TGGAAGTGCT TCTCGACTTC CTTCCAGTGT ACAGTTGGCT
    CATGTTTTTA ATGGTTCCAC AATCAAATCT TCAAGAGAGC TAGGACCCAT GGCTCCCAGT
    GGACCCGGGA TCCATGTCCG AGTTCCTCAA CTCCCAGAGG GAGAGGGTTT TGGTGGCAGT
    TCCCCTCTTC AGATTAATAA CCTGTCTAGA AAGAAGAGCC AGGCCAGGCA CACAGCAGAC
    ACGAGGACCC TTGAAGAGAA TTTCCAAGAA AAAGGAAGTG AAACGGCAGA GAGGGTCCAG
    CAGGGTTCTT CTGATGATAA TTCCCAAGAA ACTTTGCCCA CGACATCTGT CGGACAAGTG
    GAAACAAACT TGGTGCCCTT GGACTGCTCG TCAGCCAGCT CCACAGAGAG GAGAGGGCAG
    AGCTCGGGCA CTCGTGTGTC TGTGGTGGCT GAATCTCCAA TGAAAGATGA CAGTCAGCCT
    CTGAGCCAAG CTCCCCTTCT CTCTAATGTC CTTCTGGACA AGTCTAAAGA GAGAAGTCCG
    GGATGTTGGG AAGCGGGTAA AAAGCTGAAG ATCATAACTC TAGAGGCTTC TGTTTCAGAA
    ACCTGGCCAC TGGGACAGCT GACAGATTCT GGGTTCAAGG GGATGGCCGC TGGTCTCATT
    CCTGACAGGA TCCGGGCAAT TCCTGATGTT CTAAAGGCGG GTGCTGCCGT GCCTAAACCG
    GGCCCCTCTA AGACAGCATC AGCCTGCAGT CCTCAGGTAG AGTATAGCTC AGCAATTGCT
    AATAACCGAA AAATCCATAA AAGGGAAGAA CGAGCTGGCT GTGCAAGTTG GAGTTGTCTT
    TCCTCCCAGT ATTTGAGCCA ACGCAGATTC CTGGAGTCAT CTGTGGACCC TGTAGAGGGA
    AAGAAAGTCT GTGTCACAGG CTTGCTCCCA GAGGCTTTCA GAACTGGAAG GAAGGAAAAT
    GCTAACCGTG TGAGCCAAAA TCGAGATGAA AACCGACTCA AGAGGGATCG CCCCGCCTTC
    TTTAAGCAGC TCCTGTCCTG CCCTAACATC TTGGAGTCCT CTGTGGACCC CACTGATGAA
    ATGAGTGTGG GCTGGGCCAG GGTGGAAACA CCAGAGCCTT CTGAGTCCAC ACTGGGGGCC
    ATCGCAGTGG AGAATAAACC AAAGGGTGGA CACTTGGACC AGAGGCTGGA AGTCCAACCT
    GCCATCTTGC AAGTTCCGTG TGCCCAGCAA AGTGGGGAAA CCCCTCCAAG TGAAAACGGC
    ACCAACCAAA ACCAAGGGGA CTGTGTGGGA TGGGAGGCAG GACAAAGTCG CCGTGAGAGA
    GCAAAGGGGG ACATGCGTTC TGCCATTTTG CCAGTCCCAG GCGCTGTGCA GGGTGGGGAG
    GCGATGCCAG GGGCACACAT CAGAAGCCAA GTACAGGAAG ACGGGGAGAG GCGCTCAGGG
    GGGCCTGGAC GCAGTAAAAG CAACGGAGCA GCATTAGTGT CCCCCGCCCT CGCTCTCTCT
    GGCGGTCTTG CCAGGGTGAA CCCCCCATCT GTTGGAGTCA CTACTCACAG CTTCACAGCT
    CGGAGTGATG ACCTTTGTGA GGAGGACTCC GTGGAGCCCA GAAACCACGA GCATGCCTTT
    TCTGACTCAG AGGAAAGGCG AGCTATGAAG AGTGAGTGGA AGCAAGCGCC CTCTTCCAGG
    GGTCTCACCC GGGGGCCTTT TACATCCTCT CCTGAAAGAA GAGGCATCGC AGACTTTTCA
    AGAAGCCACA GAATTGAGGA ACGTGAGATA GAGGAGACCC CCGCTGGGGA AACCAAACCC
    ACAGGCACAT CTGGCTCCCC GGCGGGGCCC CTGGCATCTG TTTCCGGAGT ACATGCGTCA
    GCGAAAGCTC CTAACATGCT GCTGGATCCC TACCAGCAGG GCTCCACCCT GGGCCATGGG
    AAAAAGTCGG GGGAGGAGGA GAAGGTTGGC CCCGTGGCGG CCCAAGCCAG CCATCCACCC
    CCGCCAGCAG CGATGAAGTC ACCGGAGGTC AAGGAGAAGC AAAAAACCCC AGGAAGTGGA
    CACTTAGCTG AGGGGGTAAA GAAGAAAATT CTGTCCAGGG TGGCCGCCCT GAGGCTGAGA
    TTGGAAGAGA AGGAGAAGGT GAGAAGGCAC TCGGTCTTTG AGAGTCCTAA GCCTGAAACG
    TCGGTCTCAC GCACAGATGA GAAAGGAGAG CCCCAGAGGC CACCTTGCCA AAGCGAAGGG
    AGAGCTCCAG TATTACTGAA AAAGATCCAA GCTGAGATGT CCCCTGACCA CTCTGGAAAT
    GTAAAGTTGA GCTGCCAGTT TGCAGAGATT CATGAAGATT CTACCATCTC GTGGACAAAA
    GATTCAACAT CGATAGCCCA AGTGCAGAGA AGAGCAGGAG ACAACTCCAT GGTGAGCTTG
    GCCATCCTTC AAGCCAGTCA GAAGGACCAG GGCCTCTATT ACTGCTGCAT CAAGAACAGT
    TACGGGAGAG TGACCGCTGA GTTTAACCTC ACCGCCGAAG TTCTCAAACA GCTCTCAAGT
    CACCCAGATG TTAAAGTATA TGAAGAGATT GAATTCAGCC AGCTCATCTT CCGAGAGGAC
    TTCCTCCGTG ACAGCTACTT CGGGGGCCGC CTGCATGGCC AGATCGCCAC GGAGGAGCTG
    CACTTCGGGG AAGGCGTCCA CCGGAGAGCC TTCCGCAGCA AGGTGCTGCG CGGCCTGACG
    CCGGTCTTCA AGCCCGGCCA CGCCTGCGTG CTCAAGGTGC ACAACGCCGT CGCCTACGGG
    GCCAGAAACA ACGAGGAGCT CATCCAGAGG AACTACAGAC TCGCCGCGCA GGAATGCTAC
    GTGCAGAACA CTGCCAGGCA CTACGCGCAG ATCTACGCCG CAGAAGCCCA GCCTCTGGAA
    GGCTTCGGGG AAGTGCCCGA GATCATTCCT ATTTTCCTTA TCCATCGACC TGAGAATAAC
    ATCCCGTACG CAACCGTGGA GGAAGAGCTG ATTGGAGAAT TCGTGAAGTA TTCCATCAGG
    GATGGGAAGG AGATAAACTT CTTGAGGAGA GAGTCGGAGG CTGGGCAGAA GTGTTGCACC
    TTCCAGCACT GGGTCTACCA GAAAACAAGT GGCTGCCTGC TGGTCACGGA CATGCAGGGT
    GTAGGCATGA AGTTAACTGA TGTTGGCATA GCAACAGAGG CTACAGGGTA CAAAGGATTT
    AAAGGCAACT GTTCCATGAC CTTCATCGAT CAGTTTAAAG CCCTTCACCA GTGTAATAAG
    TACTGTAAAA TGCTGGGGTT GAAGTCGCTT CAGGACAACA GCCAGAAGCA GAAGAAGCCA
    AGCATCGGGC GGAGCAAGAT TCAGCCCGGC ACCACAGCCG TGAAGAAGGC GGCCTCCGGG
    AGCCTGGCAG AAAAGAAAAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179177.1
    CDS1..6384
    Translation

    Target ORF information:

    RefSeq Version NM_001192248.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGTTTCAA GGACAAGCCA GCACTTAACA GTGGAGTTCG AAACTGGCCA GCCCAAGCCA 
    GAGGTAACTT GGTATAAGAA TGGTCGAACC ATAGATGAAT GTGGCAGTGT CTCCAGCTAC
    GAATTCTTCG AGAATCAGTA TGTTCACGTA TTACACCTCT ATTGCTGTAC CCAAAATGAC
    ACTGCTGTTT ATCAAGTCTC TGCTCGGAAC TGTTTTGGGA TGATCTGCTG CTCTGCTTCC
    ATTGAAGTGC AGTGCCCGAC AGAGGACCAA CCACTCTCTC CCAACCCAAA AGATGACGGG
    CACACAGGTT GGAAACACGA CACGGAGACA TATGAGCAGG AGAGCCCAAA CCACACTGAT
    GAGAAAGAGC ATCCTTATAA GGAAGGAGAA GGTATCGCCT CGGGGCCTCC TACATCAGCT
    GACGCCCCCT CCTCCAAATC CAGCGGTGCT CATTCACTCC AGGTATCGGC CGACCGTGAC
    CCTGGCGCGT CTGGCTCTGA CAACCCCTTG GCGGTTAAAG ACACGAGGCA AACGGAAGAG
    GCTGGTGGTG CTGCTAACAC AGAGGGCGTT GCGGACGGGT TGCGTTTTCC TAGTTCAAGT
    GATGCTCCTG ATAAACAAGA TGTGTGTGGC CACAGGGCCG TGCATTCCGA AGTACCAAGG
    TTAACAGATG GTGCCTTAGA CCCTGAGGGC CCTAACGAGG AAGCCTTGAA CTCTAGTCAC
    CAAAATGCCA GAGTCCAGAA GTATATCAGC TTCGGCCCGG CCCTGACACG GGCAGACTCG
    TCCGCTCCTC CAGGTACCAG GCCCGTCAGC CCCCCGGCTT CCAGCACAGA CTCTGACAGT
    GACTATGAAC TTTGCCCAGA GATAACCCTA ACCTGCGCCG AGGAGTTTTC AGATGATGAC
    CTGGAGTATC TGGAATGTTC CGACGTTATG ACAGATTACT CTAATGCGAT CTGGCAAAGG
    AACCTGCAGG GGACGGAGCG TGTTTTTTTA TTAGAAAGCG ATGACGAAGA GATGGAATTC
    AGTGAGTGTA GCCTGGGTGG GTGTGCGCGT TTCTTCAGTG AATTGGGTTG CAGGCCCCCG
    GTGTCAGATG ACACGGGCCC CATGGATGCC ACCGCTGGCC TCTGTGGTCA TCACTCACCA
    CCCCAAGAAG TGGGGGGAAG GAGCAGCCGG GCCTCCACGC ACAGAGCCTC ATCCTTGCAA
    GAGGGGATGC CCCTGCCCCT GGGACTTCAG CAGGATGGAC CGGCTATGGT GACAGACCTG
    GGCAGGTATA AACCACCCGC TGCCTTGGAG GCTGCTGAAG ACGGTTATCC AGGAATTCAA
    GGAGAAACCA GAGACAGCCA CCAGGCCGGA AAGGAATTCT CTGGTGACAA GCTGCTAAAC
    ATGGATAAAG CGGTGGCAGG GACAGAGGGG AAGCACTTGT CTGGTGAGTC AGGACAGCCA
    GGGATGAGCC GGTGTCTGGA GACCAGGGCT GAGAGAAGAG TAGGGGGAAA AGACGGATGG
    AGCCAGCGGG GCTCTGAGAA ACCTGCCAGG ACGTGGCGGC CGGGAATTAA GGGAAAAGCC
    AAGAGGCTGA ATTCCAGCCT GGAAGAAAGG ACGGCGGAAG CCTCCCTGGA TCGCCTCTGT
    CCCAAGGGAC CTGTTAAGCA CCCACTAACC CCGAGTGACA AAAGAGTCAG CTCCCATGCC
    AGAGCAGAGG GGGCTGATCT GAAGCCCCAG TTGCCTGCAG GAGGCCCTGA GGTTCCAACC
    CAAGCAGAGC CAGAAGTGAA AACCCTGCAG GCTCCACCCG GCTTGCTCTC CAAGGAGGAG
    ACCTTGCACT TCCAGAGAGA AGGGGTACGG GTTACCGACG TATTTGAAAC GTGCAAGGTT
    TCAGGCTGGA GTGATCATCC TCAGGTACAA ATTCAGGAAA CAGTCAGGGA GCAAATCTCT
    CTCAGCCACA TGCCGGCTTT CTCGGAGCCT ACTGGGGAGG AGCCTACTTT TCCTGGGACC
    ACAACAGAGT CCCTCCCCAA CTTAGGAGGG ATCGACAAAG AAAGTGCATC ATTGGCCAAA
    CACCCGGAGG TAGACGGTTG TACCCAAGGG GCACGGCATG AAGAAAACCA AGGTGACAAC
    ACGCTCAGAC GTTCTGGAAG AGACCTGGGG CGTGTGCTGA GCATCCCAGA AGCCAGCAGT
    GAAGCCACGT CCCCCTGTGA GCTGTTGGGG CCTCCCCCCC AGCCCCAGGC TGCCTCTGCG
    TCCCCTGAAT TTGCACATAC TGTCCCCGCC CTGGAAACCA TGTGTGCTGC TGGGCCAGGG
    GACGGAGTAG CTGTGTGTGG CCCGGAGCGT CTTGAAGCAG GTGACCAGGA AGCATGTGAT
    ATTGTGGGCT CTCCGGTTGG AGCCCCAGTT GGTAAACATT TGCCTCAAGA AATGTGCTCC
    CTGGACTTAG AGCTGGCAGG TCAAAGCAAA CCACCTGATT TATGTCCTCC CGATGACAAG
    ACTCTGGATG TTCTCTCTCA GACACAAGGT CCTGAGCCTC CCCAGACTAC GTGCGGGAGC
    AGTGGCGAGA GAACCTCTCC CGACTCCCCT CTTTTCATCA GCACTTTCAC CTGGAACATC
    TCACAAAAAG CCAGTAAAGG TGCCACCGGG GAGGATCTAG CCAACATAGA GGGTTCCGCC
    TCCACGTCGG CCTCCATAGT AAAGGCTGGC CAGGAGAGGC TGAGCCCCAG CCACTCAGGG
    GACCTTGAGG AAAGATGGCC TCCATCTCCA GAGAGCAGCT CTTCCGTTTG GGCCATAGAG
    GGCAGTGACA AGAACTCCAG CCCCGGTGCC CCGGACTCGG CAGACATACC GGCAGGTCAT
    AGTCCAGTAA CGAAGTTTCC TCGGGAGAAG CCCACAACAC TAATTGCTGA CGTTGAGTGT
    TCTGAGGTGA CTGGGGCGAA TAGCAACACA TCTGCTCTTT CCGTTGCCCC GAAAGGCCAC
    CCATCCAAAT ACCTCGCTGT TTCAGTTGCT GGGGACAGCC ATGCAGGTGG CCCTGAGGAG
    AGTTCACCTC GGGCACCAGA TGGAAGTGCT TCTCGACTTC CTTCCAGTGT ACAGTTGGCT
    CATGTTTTTA ATGGTTCCAC AATCAAATCT TCAAGAGAGC TAGGACCCAT GGCTCCCAGT
    GGACCCGGGA TCCATGTCCG AGTTCCTCAA CTCCCAGAGG GAGAGGGTTT TGGTGGCAGT
    TCCCCTCTTC AGATTAATAA CCTGTCTAGA AAGAAGAGCC AGGCCAGGCA CACAGCAGAC
    ACGAGGACCC TTGAAGAGAA TTTCCAAGAA AAAGGAAGTG AAACGGCAGA GAGGGTCCAG
    CAGGGTTCTT CTGATGATAA TTCCCAAGAA ACTTTGCCCA CGACATCTGT CGGACAAGTG
    GAAACAAACT TGGTGCCCTT GGACTGCTCG TCAGCCAGCT CCACAGAGAG GAGAGGGCAG
    AGCTCGGGCA CTCGTGTGTC TGTGGTGGCT GAATCTCCAA TGAAAGATGA CAGTCAGCCT
    CTGAGCCAAG CTCCCCTTCT CTCTAATGTC CTTCTGGACA AGTCTAAAGA GAGAAGTCCG
    GGATGTTGGG AAGCGGGTAA AAAGCTGAAG ATCATAACTC TAGAGGCTTC TGTTTCAGAA
    ACCTGGCCAC TGGGACAGCT GACAGATTCT GGGTTCAAGG GGATGGCCGC TGGTCTCATT
    CCTGACAGGA TCCGGGCAAT TCCTGATGTT CTAAAGGCGG GTGCTGCCGT GCCTAAACCG
    GGCCCCTCTA AGACAGCATC AGCCTGCAGT CCTCAGGTAG AGTATAGCTC AGCAATTGCT
    AATAACCGAA AAATCCATAA AAGGGAAGAA CGAGCTGGCT GTGCAAGTTG GAGTTGTCTT
    TCCTCCCAGT ATTTGAGCCA ACGCAGATTC CTGGAGTCAT CTGTGGACCC TGTAGAGGGA
    AAGAAAGTCT GTGTCACAGG CTTGCTCCCA GAGGCTTTCA GAACTGGAAG GAAGGAAAAT
    GCTAACCGTG TGAGCCAAAA TCGAGATGAA AACCGACTCA AGAGGGATCG CCCCGCCTTC
    TTTAAGCAGC TCCTGTCCTG CCCTAACATC TTGGAGTCCT CTGTGGACCC CACTGATGAA
    ATGAGTGTGG GCTGGGCCAG GGTGGAAACA CCAGAGCCTT CTGAGTCCAC ACTGGGGGCC
    ATCGCAGTGG AGAATAAACC AAAGGGTGGA CACTTGGACC AGAGGCTGGA AGTCCAACCT
    GCCATCTTGC AAGTTCCGTG TGCCCAGCAA AGTGGGGAAA CCCCTCCAAG TGAAAACGGC
    ACCAACCAAA ACCAAGGGGA CTGTGTGGGA TGGGAGGCAG GACAAAGTCG CCGTGAGAGA
    GCAAAGGGGG ACATGCGTTC TGCCATTTTG CCAGTCCCAG GCGCTGTGCA GGGTGGGGAG
    GCGATGCCAG GGGCACACAT CAGAAGCCAA GTACAGGAAG ACGGGGAGAG GCGCTCAGGG
    GGGCCTGGAC GCAGTAAAAG CAACGGAGCA GCATTAGTGT CCCCCGCCCT CGCTCTCTCT
    GGCGGTCTTG CCAGGGTGAA CCCCCCATCT GTTGGAGTCA CTACTCACAG CTTCACAGCT
    CGGAGTGATG ACCTTTGTGA GGAGGACTCC GTGGAGCCCA GAAACCACGA GCATGCCTTT
    TCTGACTCAG AGGAAAGGCG AGCTATGAAG AGTGAGTGGA AGCAAGCGCC CTCTTCCAGG
    GGTCTCACCC GGGGGCCTTT TACATCCTCT CCTGAAAGAA GAGGCATCGC AGACTTTTCA
    AGAAGCCACA GAATTGAGGA ACGTGAGATA GAGGAGACCC CCGCTGGGGA AACCAAACCC
    ACAGGCACAT CTGGCTCCCC GGCGGGGCCC CTGGCATCTG TTTCCGGAGT ACATGCGTCA
    GCGAAAGCTC CTAACATGCT GCTGGATCCC TACCAGCAGG GCTCCACCCT GGGCCATGGG
    AAAAAGTCGG GGGAGGAGGA GAAGGTTGGC CCCGTGGCGG CCCAAGCCAG CCATCCACCC
    CCGCCAGCAG CGATGAAGTC ACCGGAGGTC AAGGAGAAGC AAAAAACCCC AGGAAGTGGA
    CACTTAGCTG AGGGGGTAAA GAAGAAAATT CTGTCCAGGG TGGCCGCCCT GAGGCTGAGA
    TTGGAAGAGA AGGAGAAGGT GAGAAGGCAC TCGGTCTTTG AGAGTCCTAA GCCTGAAACG
    TCGGTCTCAC GCACAGATGA GAAAGGAGAG CCCCAGAGGC CACCTTGCCA AAGCGAAGGG
    AGAGCTCCAG TATTACTGAA AAAGATCCAA GCTGAGATGT CCCCTGACCA CTCTGGAAAT
    GTAAAGTTGA GCTGCCAGTT TGCAGAGATT CATGAAGATT CTACCATCTC GTGGACAAAA
    GATTCAACAT CGATAGCCCA AGTGCAGAGA AGAGCAGGAG ACAACTCCAT GGTGAGCTTG
    GCCATCCTTC AAGCCAGTCA GAAGGACCAG GGCCTCTATT ACTGCTGCAT CAAGAACAGT
    TACGGGAGAG TGACCGCTGA GTTTAACCTC ACCGCCGAAG TTCTCAAACA GCTCTCAAGT
    CACCCAGATG TTAAAGTATA TGAAGAGATT GAATTCAGCC AGCTCATCTT CCGAGAGGAC
    TTCCTCCGTG ACAGCTACTT CGGGGGCCGC CTGCATGGCC AGATCGCCAC GGAGGAGCTG
    CACTTCGGGG AAGGCGTCCA CCGGAGAGCC TTCCGCAGCA AGGTGCTGCG CGGCCTGACG
    CCGGTCTTCA AGCCCGGCCA CGCCTGCGTG CTCAAGGTGC ACAACGCCGT CGCCTACGGG
    GCCAGAAACA ACGAGGAGCT CATCCAGAGG AACTACAGAC TCGCCGCGCA GGAATGCTAC
    GTGCAGAACA CTGCCAGGCA CTACGCGCAG ATCTACGCCG CAGAAGCCCA GCCTCTGGAA
    GGCTTCGGGG AAGTGCCCGA GATCATTCCT ATTTTCCTTA TCCATCGACC TGAGAATAAC
    ATCCCGTACG CAACCGTGGA GGAAGAGCTG ATTGGAGAAT TCGTGAAGTA TTCCATCAGG
    GATGGGAAGG AGATAAACTT CTTGAGGAGA GAGTCGGAGG CTGGGCAGAA GTGTTGCACC
    TTCCAGCACT GGGTCTACCA GAAAACAAGT GGCTGCCTGC TGGTCACGGA CATGCAGGGT
    GTAGGCATGA AGTTAACTGA TGTTGGCATA GCAACAGAGG CTACAGGGTA CAAAGGATTT
    AAAGGCAACT GTTCCATGAC CTTCATCGAT CAGTTTAAAG CCCTTCACCA GTGTAATAAG
    TACTGTAAAA TGCTGGGGTT GAAGTCGCTT CAGGACAACA GCCAGAAGCA GAAGAAGCCA
    AGCATCGGGC GGAGCAAGAT TCAGCCCGGC ACCACAGCCG TGAAGAAGGC GGCCTCCGGG
    AGCCTGGCAG AAAAGAAAAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32523
    Clone ID Related Accession (Same CDS sequence) XM_005224149.3 , XM_005224149.4
    Accession Version XM_005224149.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGTT
    CTCAAACAGC TCTCAAGTCA CCCAGATGTT AAAGTATATG AAGAGATTGA ATTCAGCCAG
    CTCATCTTCC GAGAGGACTT CCTCCGTGAC AGCTACTTCG GGGGCCGCCT GCATGGCCAG
    ATCGCCACGG AGGAGCTGCA CTTCGGGGAA GGCGTCCACC GGAGAGCCTT CCGCAGCAAG
    GTGCTGCGCG GCCTGACGCC GGTCTTCAAG CCCGGCCACG CCTGCGTGCT CAAGGTGCAC
    AACGCCGTCG CCTACGGGGC CAGAAACAAC GAGGAGCTCA TCCAGAGGAA CTACAGACTC
    GCCGCGCAGG AATGCTACGT GCAGAACACT GCCAGGCACT ACGCGCAGAT CTACGCCGCA
    GAAGCCCAGC CTCTGGAAGG CTTCGGGGAA GTGCCCGAGA TCATTCCTAT TTTCCTTATC
    CATCGACCTG AGAATAACAT CCCGTACGCA ACCGTGGAGG AAGAGCTGAT TGGAGAATTC
    GTGAAGTATT CCATCAGGGA TGGGAAGGAG ATAAACTTCT TGAGGAGAGA GTCGGAGGCT
    GGGCAGAAGT GTTGCACCTT CCAGCACTGG GTCTACCAGA AAACAAGTGG CTGCCTGCTG
    GTCACGGACA TGCAGGGTGT AGGCATGAAG TTAACTGATG TTGGCATAGC AACAGAGGCT
    ACAGGGTACA AAGGATTTAA AGGCAACTGT TCCATGACCT TCATCGATCA GTTTAAAGCC
    CTTCACCAGT GTAATAAGTA CTGTAAAATG CTGGGGTTGA AGTCGCTTCA GGACAACAGC
    CAGAAGCAGA AGAAGCCAAG CATCGGGCGG AGCAAGATTC AGCCCGGCAC CACAGCCGTG
    AAGAAGGCGG CCTCCGGGAG CCTGGCAGAA AAGAAAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224206.1
    CDS93..6554
    Translation

    Target ORF information:

    RefSeq Version XM_005224149.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224149.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGTT
    CTCAAACAGC TCTCAAGTCA CCCAGATGTT AAAGTATATG AAGAGATTGA ATTCAGCCAG
    CTCATCTTCC GAGAGGACTT CCTCCGTGAC AGCTACTTCG GGGGCCGCCT GCATGGCCAG
    ATCGCCACGG AGGAGCTGCA CTTCGGGGAA GGCGTCCACC GGAGAGCCTT CCGCAGCAAG
    GTGCTGCGCG GCCTGACGCC GGTCTTCAAG CCCGGCCACG CCTGCGTGCT CAAGGTGCAC
    AACGCCGTCG CCTACGGGGC CAGAAACAAC GAGGAGCTCA TCCAGAGGAA CTACAGACTC
    GCCGCGCAGG AATGCTACGT GCAGAACACT GCCAGGCACT ACGCGCAGAT CTACGCCGCA
    GAAGCCCAGC CTCTGGAAGG CTTCGGGGAA GTGCCCGAGA TCATTCCTAT TTTCCTTATC
    CATCGACCTG AGAATAACAT CCCGTACGCA ACCGTGGAGG AAGAGCTGAT TGGAGAATTC
    GTGAAGTATT CCATCAGGGA TGGGAAGGAG ATAAACTTCT TGAGGAGAGA GTCGGAGGCT
    GGGCAGAAGT GTTGCACCTT CCAGCACTGG GTCTACCAGA AAACAAGTGG CTGCCTGCTG
    GTCACGGACA TGCAGGGTGT AGGCATGAAG TTAACTGATG TTGGCATAGC AACAGAGGCT
    ACAGGGTACA AAGGATTTAA AGGCAACTGT TCCATGACCT TCATCGATCA GTTTAAAGCC
    CTTCACCAGT GTAATAAGTA CTGTAAAATG CTGGGGTTGA AGTCGCTTCA GGACAACAGC
    CAGAAGCAGA AGAAGCCAAG CATCGGGCGG AGCAAGATTC AGCCCGGCAC CACAGCCGTG
    AAGAAGGCGG CCTCCGGGAG CCTGGCAGAA AAGAAAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32523
    Clone ID Related Accession (Same CDS sequence) XM_005224149.3 , XM_005224149.4
    Accession Version XM_005224149.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224149.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGTT
    CTCAAACAGC TCTCAAGTCA CCCAGATGTT AAAGTATATG AAGAGATTGA ATTCAGCCAG
    CTCATCTTCC GAGAGGACTT CCTCCGTGAC AGCTACTTCG GGGGCCGCCT GCATGGCCAG
    ATCGCCACGG AGGAGCTGCA CTTCGGGGAA GGCGTCCACC GGAGAGCCTT CCGCAGCAAG
    GTGCTGCGCG GCCTGACGCC GGTCTTCAAG CCCGGCCACG CCTGCGTGCT CAAGGTGCAC
    AACGCCGTCG CCTACGGGGC CAGAAACAAC GAGGAGCTCA TCCAGAGGAA CTACAGACTC
    GCCGCGCAGG AATGCTACGT GCAGAACACT GCCAGGCACT ACGCGCAGAT CTACGCCGCA
    GAAGCCCAGC CTCTGGAAGG CTTCGGGGAA GTGCCCGAGA TCATTCCTAT TTTCCTTATC
    CATCGACCTG AGAATAACAT CCCGTACGCA ACCGTGGAGG AAGAGCTGAT TGGAGAATTC
    GTGAAGTATT CCATCAGGGA TGGGAAGGAG ATAAACTTCT TGAGGAGAGA GTCGGAGGCT
    GGGCAGAAGT GTTGCACCTT CCAGCACTGG GTCTACCAGA AAACAAGTGG CTGCCTGCTG
    GTCACGGACA TGCAGGGTGT AGGCATGAAG TTAACTGATG TTGGCATAGC AACAGAGGCT
    ACAGGGTACA AAGGATTTAA AGGCAACTGT TCCATGACCT TCATCGATCA GTTTAAAGCC
    CTTCACCAGT GTAATAAGTA CTGTAAAATG CTGGGGTTGA AGTCGCTTCA GGACAACAGC
    CAGAAGCAGA AGAAGCCAAG CATCGGGCGG AGCAAGATTC AGCCCGGCAC CACAGCCGTG
    AAGAAGGCGG CCTCCGGGAG CCTGGCAGAA AAGAAAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224206.1
    CDS648..7109
    Translation

    Target ORF information:

    RefSeq Version XM_005224149.4
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 2 (ALPK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224149.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGTGGCATG AAAGGATGGC AGACTCAGGA GGGCCCCAGA GGCGCGCGCT TTGTTTTTTG 
    TCTACGTTGC TTTCCCAGAA AGTTCCTGAG AAGTCCGATG CCGTGCTTCG TTGCACAATA
    TCAGGCCAGC CCAAGCCAGA GGTAACTTGG TATAAGAATG GTCGAACCAT AGATGAATGT
    GGCAGTGTCT CCAGCTACGA ATTCTTCGAG AATCAGTATG TTCACGTATT ACACCTCTAT
    TGCTGTACCC AAAATGACAC TGCTGTTTAT CAAGTCTCTG CTCGGAACTG TTTTGGGATG
    ATCTGCTGCT CTGCTTCCAT TGAAGTGCAG TGCCCGACAG AGGACCAACC ACTCTCTCCC
    AACCCAAAAG ATGACGGGCA CACAGGTTGG AAACACGACA CGGAGACATA TGAGCAGGAG
    AGCCCAAACC ACACTGATGA GAAAGAGCAT CCTTATAAGG AAGGAGAAGG TATCGCCTCG
    GGGCCTCCTA CATCAGCTGA CGCCCCCTCC TCCAAATCCA GCGGTGCTCA TTCACTCCAG
    GTATCGGCCG ACCGTGACCC TGGCGCGTCT GGCTCTGACA ACCCCTTGGC GGTTAAAGAC
    ACGAGGCAAA CGGAAGAGGC TGGTGGTGCT GCTAACACAG AGGGCGTTGC GGACGGGTTG
    CGTTTTCCTA GTTCAAGTGA TGCTCCTGAT AAACAAGATG TGTGTGGCCA CAGGGCCGTG
    CATTCCGAAG TACCAAGGTT AACAGATGGT GCCTTAGACC CTGAGGGCCC TAACGAGGAA
    GCCTTGAACT CTAGTCACCA AAATGCCAGA GTCCAGAAGT ATATCAGCTT CGGCCCGGCC
    CTGACACGGG CAGACTCGTC CGCTCCTCCA GGTACCAGGC CCGTCAGCCC CCCGGCTTCC
    AGCACAGACT CTGACAGTGA CTATGAACTT TGCCCAGAGA TAACCCTAAC CTGCGCCGAG
    GAGTTTTCAG ATGATGACCT GGAGTATCTG GAATGTTCCG ACGTTATGAC AGATTACTCT
    AATGCGATCT GGCAAAGGAA CCTGCAGGGG ACGGAGCGTG TTTTTTTATT AGAAAGCGAT
    GACGAAGAGA TGGAATTCAG TGAGTGTAGC CTGGGTGGGT GTGCGCGTTT CTTCAGTGAA
    TTGGGTTGCA GGCCCCCGGT GTCAGATGAC ACGGGCCCCA TGGATGCCAC CGCTGGCCTC
    TGTGGTCATC ACTCACCACC CCAAGAAGTG GGGGGAAGGA GCAGCCGGGC CTCCACGCAC
    AGAGCCTCAT CCTTGCAAGA GGGGATGCCC CTGCCCCTGG GACTTCAGCA GGATGGACCG
    GCTATGGTGA CAGACCTGGG CAGGTATAAA CCACCCGCTG CCTTGGAGGC TGCTGAAGAC
    GGTTATCCAG GAATTCAAGG AGAAACCAGA GACAGCCACC AGGCCGGAAA GGAATTCTCT
    GGTGACAAGC TGCTAAACAT GGATAAAGCG GTGGCAGGGA CAGAGGGGAA GCACTTGTCT
    GGTGAGTCAG GACAGCCAGG GATGAGCCGG TGTCTGGAGA CCAGGGCTGA GAGAAGAGTA
    GGGGGAAAAG ACGGATGGAG CCAGCGGGGC TCTGAGAAAC CTGCCAGGAC GTGGCGGCCG
    GGAATTAAGG GAAAAGCCAA GAGGCTGAAT TCCAGCCTGG AAGAAAGGAC GGCGGAAGCC
    TCCCTGGATC GCCTCTGTCC CAAGGGACCT GTTAAGCACC CACTAACCCC GAGTGACAAA
    AGAGTCAGCT CCCATGCCAG AGCAGAGGGG GCTGATCTGA AGCCCCAGTT GCCTGCAGGA
    GGCCCTGAGG TTCCAACCCA AGCAGAGCCA GAAGTGAAAA CCCTGCAGGC TCCACCCGGC
    TTGCTCTCCA AGGAGGAGAC CTTGCACTTC CAGAGAGAAG GGGTACGGGT TACCGACGTA
    TTTGAAACGT GCAAGGTTTC AGGCTGGAGT GATCATCCTC AGGTACAAAT TCAGGAAACA
    GTCAGGGAGC AAATCTCTCT CAGCCACATG CCGGCTTTCT CGGAGCCTAC TGGGGAGGAG
    CCTACTTTTC CTGGGACCAC AACAGAGTCC CTCCCCAACT TAGGAGGGAT CGACAAAGAA
    AGTGCATCAT TGGCCAAACA CCCGGAGGTA GACGGTTGTA CCCAAGGGGC ACGGCATGAA
    GAAAACCAAG GTGACAACAC GCTCAGACGT TCTGGAAGAG ACCTGGGGCG TGTGCTGAGC
    ATCCCAGAAG CCAGCAGTGA AGCCACGTCC CCCTGTGAGC TGTTGGGGCC TCCCCCCCAG
    CCCCAGGCTG CCTCTGCGTC CCCTGAATTT GCACATACTG TCCCCGCCCT GGAAACCATG
    TGTGCTGCTG GGCCAGGGGA CGGAGTAGCT GTGTGTGGCC CGGAGCGTCT TGAAGCAGGT
    GACCAGGAAG CATGTGATAT TGTGGGCTCT CCGGTTGGAG CCCCAGTTGG TAAACATTTG
    CCTCAAGAAA TGTGCTCCCT GGACTTAGAG CTGGCAGGTC AAAGCAAACC ACCTGATTTA
    TGTCCTCCCG ATGACAAGAC TCTGGATGTT CTCTCTCAGA CACAAGGTCC TGAGCCTCCC
    CAGACTACGT GCGGGAGCAG TGGCGAGAGA ACCTCTCCCG ACTCCCCTCT TTTCATCAGC
    ACTTTCACCT GGAACATCTC ACAAAAAGCC AGTAAAGGTG CCACCGGGGA GGATCTAGCC
    AACATAGAGG GTTCCGCCTC CACGTCGGCC TCCATAGTAA AGGCTGGCCA GGAGAGGCTG
    AGCCCCAGCC ACTCAGGGGA CCTTGAGGAA AGATGGCCTC CATCTCCAGA GAGCAGCTCT
    TCCGTTTGGG CCATAGAGGG CAGTGACAAG AACTCCAGCC CCGGTGCCCC GGACTCGGCA
    GACATACCGG CAGGTCATAG TCCAGTAACG AAGTTTCCTC GGGAGAAGCC CACAACACTA
    ATTGCTGACG TTGAGTGTTC TGAGGTGACT GGGGCGAATA GCAACACATC TGCTCTTTCC
    GTTGCCCCGA AAGGCCACCC ATCCAAATAC CTCGCTGTTT CAGTTGCTGG GGACAGCCAT
    GCAGGTGGCC CTGAGGAGAG TTCACCTCGG GCACCAGATG GAAGTGCTTC TCGACTTCCT
    TCCAGTGTAC AGTTGGCTCA TGTTTTTAAT GGTTCCACAA TCAAATCTTC AAGAGAGCTA
    GGACCCATGG CTCCCAGTGG ACCCGGGATC CATGTCCGAG TTCCTCAACT CCCAGAGGGA
    GAGGGTTTTG GTGGCAGTTC CCCTCTTCAG ATTAATAACC TGTCTAGAAA GAAGAGCCAG
    GCCAGGCACA CAGCAGACAC GAGGACCCTT GAAGAGAATT TCCAAGAAAA AGGAAGTGAA
    ACGGCAGAGA GGGTCCAGCA GGGTTCTTCT GATGATAATT CCCAAGAAAC TTTGCCCACG
    ACATCTGTCG GACAAGTGGA AACAAACTTG GTGCCCTTGG ACTGCTCGTC AGCCAGCTCC
    ACAGAGAGGA GAGGGCAGAG CTCGGGCACT CGTGTGTCTG TGGTGGCTGA ATCTCCAATG
    AAAGATGACA GTCAGCCTCT GAGCCAAGCT CCCCTTCTCT CTAATGTCCT TCTGGACAAG
    TCTAAAGAGA GAAGTCCGGG ATGTTGGGAA GCGGGTAAAA AGCTGAAGAT CATAACTCTA
    GAGGCTTCTG TTTCAGAAAC CTGGCCACTG GGACAGCTGA CAGATTCTGG GTTCAAGGGG
    ATGGCCGCTG GTCTCATTCC TGACAGGATC CGGGCAATTC CTGATGTTCT AAAGGCGGGT
    GCTGCCGTGC CTAAACCGGG CCCCTCTAAG ACAGCATCAG CCTGCAGTCC TCAGGTAGAG
    TATAGCTCAG CAATTGCTAA TAACCGAAAA ATCCATAAAA GGGAAGAACG AGCTGGCTGT
    GCAAGTTGGA GTTGTCTTTC CTCCCAGTAT TTGAGCCAAC GCAGATTCCT GGAGTCATCT
    GTGGACCCTG TAGAGGGAAA GAAAGTCTGT GTCACAGGCT TGCTCCCAGA GGCTTTCAGA
    ACTGGAAGGA AGGAAAATGC TAACCGTGTG AGCCAAAATC GAGATGAAAA CCGACTCAAG
    AGGGATCGCC CCGCCTTCTT TAAGCAGCTC CTGTCCTGCC CTAACATCTT GGAGTCCTCT
    GTGGACCCCA CTGATGAAAT GAGTGTGGGC TGGGCCAGGG TGGAAACACC AGAGCCTTCT
    GAGTCCACAC TGGGGGCCAT CGCAGTGGAG AATAAACCAA AGGGTGGACA CTTGGACCAG
    AGGCTGGAAG TCCAACCTGC CATCTTGCAA GTTCCGTGTG CCCAGCAAAG TGGGGAAACC
    CCTCCAAGTG AAAACGGCAC CAACCAAAAC CAAGGGGACT GTGTGGGATG GGAGGCAGGA
    CAAAGTCGCC GTGAGAGAGC AAAGGGGGAC ATGCGTTCTG CCATTTTGCC AGTCCCAGGC
    GCTGTGCAGG GTGGGGAGGC GATGCCAGGG GCACACATCA GAAGCCAAGT ACAGGAAGAC
    GGGGAGAGGC GCTCAGGGGG GCCTGGACGC AGTAAAAGCA ACGGAGCAGC ATTAGTGTCC
    CCCGCCCTCG CTCTCTCTGG CGGTCTTGCC AGGGTGAACC CCCCATCTGT TGGAGTCACT
    ACTCACAGCT TCACAGCTCG GAGTGATGAC CTTTGTGAGG AGGACTCCGT GGAGCCCAGA
    AACCACGAGC ATGCCTTTTC TGACTCAGAG GAAAGGCGAG CTATGAAGAG TGAGTGGAAG
    CAAGCGCCCT CTTCCAGGGG TCTCACCCGG GGGCCTTTTA CATCCTCTCC TGAAAGAAGA
    GGCATCGCAG ACTTTTCAAG AAGCCACAGA ATTGAGGAAC GTGAGATAGA GGAGACCCCC
    GCTGGGGAAA CCAAACCCAC AGGCACATCT GGCTCCCCGG CGGGGCCCCT GGCATCTGTT
    TCCGGAGTAC ATGCGTCAGC GAAAGCTCCT AACATGCTGC TGGATCCCTA CCAGCAGGGC
    TCCACCCTGG GCCATGGGAA AAAGTCGGGG GAGGAGGAGA AGGTTGGCCC CGTGGCGGCC
    CAAGCCAGCC ATCCACCCCC GCCAGCAGCG ATGAAGTCAC CGGAGGTCAA GGAGAAGCAA
    AAAACCCCAG GAAGTGGACA CTTAGCTGAG GGGGTAAAGA AGAAAATTCT GTCCAGGGTG
    GCCGCCCTGA GGCTGAGATT GGAAGAGAAG GAGAAGGTGA GAAGGCACTC GGTCTTTGAG
    AGTCCTAAGC CTGAAACGTC GGTCTCACGC ACAGATGAGA AAGGAGAGCC CCAGAGGCCA
    CCTTGCCAAA GCGAAGGGAG AGCTCCAGTA TTACTGAAAA AGATCCAAGC TGAGATGTCC
    CCTGACCACT CTGGAAATGT AAAGTTGAGC TGCCAGTTTG CAGAGATTCA TGAAGATTCT
    ACCATCTCGT GGACAAAAGA TTCAACATCG ATAGCCCAAG TGCAGAGAAG AGCAGGAGAC
    AACTCCATGG TGAGCTTGGC CATCCTTCAA GCCAGTCAGA AGGACCAGGG CCTCTATTAC
    TGCTGCATCA AGAACAGTTA CGGGAGAGTG ACCGCTGAGT TTAACCTCAC CGCCGAAGTT
    CTCAAACAGC TCTCAAGTCA CCCAGATGTT AAAGTATATG AAGAGATTGA ATTCAGCCAG
    CTCATCTTCC GAGAGGACTT CCTCCGTGAC AGCTACTTCG GGGGCCGCCT GCATGGCCAG
    ATCGCCACGG AGGAGCTGCA CTTCGGGGAA GGCGTCCACC GGAGAGCCTT CCGCAGCAAG
    GTGCTGCGCG GCCTGACGCC GGTCTTCAAG CCCGGCCACG CCTGCGTGCT CAAGGTGCAC
    AACGCCGTCG CCTACGGGGC CAGAAACAAC GAGGAGCTCA TCCAGAGGAA CTACAGACTC
    GCCGCGCAGG AATGCTACGT GCAGAACACT GCCAGGCACT ACGCGCAGAT CTACGCCGCA
    GAAGCCCAGC CTCTGGAAGG CTTCGGGGAA GTGCCCGAGA TCATTCCTAT TTTCCTTATC
    CATCGACCTG AGAATAACAT CCCGTACGCA ACCGTGGAGG AAGAGCTGAT TGGAGAATTC
    GTGAAGTATT CCATCAGGGA TGGGAAGGAG ATAAACTTCT TGAGGAGAGA GTCGGAGGCT
    GGGCAGAAGT GTTGCACCTT CCAGCACTGG GTCTACCAGA AAACAAGTGG CTGCCTGCTG
    GTCACGGACA TGCAGGGTGT AGGCATGAAG TTAACTGATG TTGGCATAGC AACAGAGGCT
    ACAGGGTACA AAGGATTTAA AGGCAACTGT TCCATGACCT TCATCGATCA GTTTAAAGCC
    CTTCACCAGT GTAATAAGTA CTGTAAAATG CTGGGGTTGA AGTCGCTTCA GGACAACAGC
    CAGAAGCAGA AGAAGCCAAG CATCGGGCGG AGCAAGATTC AGCCCGGCAC CACAGCCGTG
    AAGAAGGCGG CCTCCGGGAG CCTGGCAGAA AAGAAAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.