USP28 cDNA ORF clone, Bos taurus(cattle)

The following USP28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa222702 XM_024975129.1
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222700 XM_024975127.1
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222701 XM_024975128.1
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa92628 XM_015474609.2
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa92629 XM_015474610.2
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa40337 NM_001192998.1
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa26216 XM_005215811.4
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa26217 XM_005215812.4
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa26219 XM_005215814.4
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa26221 XM_005215816.4
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa26222 XM_005215817.4
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47296 XM_010812360.2
Latest version!
Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa26216 XM_005215811.3 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa26217 XM_005215812.3 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa26222 XM_005215817.3 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa26219 XM_005215814.3 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa92629 XM_015474610.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa92628 XM_015474609.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa26221 XM_005215816.3 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa222702
    Clone ID Related Accession (Same CDS sequence) XM_024975129.1
    Accession Version XM_024975129.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTC 
    AATCAGTCCC TGGGTCAGCC AGAGAAAATT CACAATAAGC TTGAATTTCC TCAGATCATT
    TATATGGACA GGTATATGTA CAGGAGCAAA GAGCTTGTTC GAAGTAAGCG AGATTGTATT
    CGAAAACTCA AAGAAGAAAT AAAAATTCTG CAGCAAAAAC TGGAAAGGTA TGTGAAGTAT
    GGCTCGGGCC CAGCCCGGTT CCCGCTTCCG GACATGTTGA AATACGTGAT TGAATTTGCT
    AGTACAAAAC CTGCCTCAGA GAGCTCTGCA TCTCAAAGTG ATGCGTGCAT GACAGAACCA
    CTTTCTTCAG CACACTGCCT GACTTCTGAT CTGACATCCA AGGAAAGTAC AAGTGAAGAG
    AGCACGTCTC AGGAAGCTGA AGGAACCTTT TCTTCCTCTG AAGATTCTGG ACGCAAGTCT
    AGGATGGCAG AGCAGCCACT TAAACCTCCC CGTTCTTCCG TGGAAATGCC CGCACACCCA
    GCTCCTCGAA CAGTCACAGA TGAGGAGATA AACTTTGTTA AGACCTGCCT GCAGAGGTGG
    AGGAACGAGA TTGAACAGGA TATACAAGAC TTAAAGAACA GTATTGCAAG CACTACCCAG
    ACTATTGAGC AGATGTACTG TGATCCTCTC CTCCGTCAGG TGCCTTATCG CTTGCACGCG
    GTCCTCGTTC ACGAAGGACA GGCAAACGCG GGGCACTACT GGGCCTACGT CTATAATCAG
    CCGCGACAAG TCTGGCTCAA GTACAACGAC ATCTCTGTTA CTGAGTCTTC CTGGGAAGAA
    CTTGAAAGAG ATTCCTATGG GGGCCAGAGA AATGTTAGTG CTTACTGCCT GATGTATATT
    AACGACAAGC TGCCCCACTT CAATGCAGAG GCGGCCCCAA ATGAATTAGA TCAGATGTCA
    GGAGAAGTAG AAGCCCTGTC TGTTGAACTT AAGCATTACA TTCAGGAAGA TAACTGGAGG
    TTTGAGCAGG AAGTGGAGGA ATGGGAAGAA GAGCAGTCTT GTAAAATTCC TCAGATGGCA
    TCTTCCACCA GCTCAGCATC ACAGGACTTC TCTCCATCTC AAGAATCTTC AGTAGCCTCT
    TCCCACGGGG CTCGGTGCCT GTCCTCTGAG CACGCTGTGA TCGTGAAGGA GCAGACTGCC
    CAGGCGATTG CAAACACAGC ACATGCCTAT GAGAACAGTG GCGTAGAAGC TGCACTGAGT
    GAGCTTAAGG AAGCTGAACC CAAGAAGCCC ATGCCCCCGG AAACAAACCT TGCAGAGCAG
    TCAGAGCAGC CCCCACAGGC TCGTGACGCA GAGTCTGCTG CCCAGCCTAA TGCTGAGGTC
    TCTGAAGTCG AGATTCCCAG TGTGGGAAGG ATTCTGGTTA GATCTGATGC AGATGGATAT
    GATGAGGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC
    CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTTA TTAAGGCATT CCATGAAGAG
    TACTCCAGGC TCTATCAGCT CGCCAAGGAG ACCCCCACCT CTCACAGTGA TCCTCGTCTT
    CAGCACGTGC TCATCTACTT CTTCCAAAAT GAAGCGCCTA AAAGGGTAGT AGAGCGGACC
    CTTCTGGAAC AATTTGCCGA TAAAAACCTT AGCTATGATG AAAGATCGAT CAGTATTATG
    AAGGTGGCTC AAGCAAAACT GAAGGAGATT GGTCCAGATG ACATGAATAT GGAAGAGTAC
    AAGAAGTGGC ATGAAGATTA CAGTTTGTTT CGAAAGGTGT CTGTGTATCT GCTGACAGGC
    CTGGAACTCT ATCAGAAAGG AAAGTACCAA GAAGCGCTTT CCTACCTGGT GTATGCCTAC
    CAAAGTAATG CCACTCTGCT GATGAAGGGG CCCCAGCGGG GCGTAAAGGA GTCGGTGATC
    GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTCTTTGAA
    ACAAATGATG AGCACTCTGT AACAGAGGGT ATTAATGTGA TGAATGAGCT GATCATTCCC
    TGTATTCACC TTATCATTAA TAATGACATC TCCAAGGATG ACCTGGATGC CATTGAGGTC
    ATGAGAAACC ACTGGTGCTC TTACCTTGGG CAGGATATCG CAGAAAATCT GCAGCTGTGC
    TTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATTGT CTTAAAGGAG
    CCTCCAACTA TTCGGCCCAA TTCTCCCTAT GACCTTTGTA GCCGATTTGC AGCTGTCATG
    GAGTCAATTC AAGGAGTGTC AGCTGTGACA GTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830897.1
    CDS1167..3545
    Translation

    Target ORF information:

    RefSeq Version XM_024975129.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTC 
    AATCAGTCCC TGGGTCAGCC AGAGAAAATT CACAATAAGC TTGAATTTCC TCAGATCATT
    TATATGGACA GGTATATGTA CAGGAGCAAA GAGCTTGTTC GAAGTAAGCG AGATTGTATT
    CGAAAACTCA AAGAAGAAAT AAAAATTCTG CAGCAAAAAC TGGAAAGGTA TGTGAAGTAT
    GGCTCGGGCC CAGCCCGGTT CCCGCTTCCG GACATGTTGA AATACGTGAT TGAATTTGCT
    AGTACAAAAC CTGCCTCAGA GAGCTCTGCA TCTCAAAGTG ATGCGTGCAT GACAGAACCA
    CTTTCTTCAG CACACTGCCT GACTTCTGAT CTGACATCCA AGGAAAGTAC AAGTGAAGAG
    AGCACGTCTC AGGAAGCTGA AGGAACCTTT TCTTCCTCTG AAGATTCTGG ACGCAAGTCT
    AGGATGGCAG AGCAGCCACT TAAACCTCCC CGTTCTTCCG TGGAAATGCC CGCACACCCA
    GCTCCTCGAA CAGTCACAGA TGAGGAGATA AACTTTGTTA AGACCTGCCT GCAGAGGTGG
    AGGAACGAGA TTGAACAGGA TATACAAGAC TTAAAGAACA GTATTGCAAG CACTACCCAG
    ACTATTGAGC AGATGTACTG TGATCCTCTC CTCCGTCAGG TGCCTTATCG CTTGCACGCG
    GTCCTCGTTC ACGAAGGACA GGCAAACGCG GGGCACTACT GGGCCTACGT CTATAATCAG
    CCGCGACAAG TCTGGCTCAA GTACAACGAC ATCTCTGTTA CTGAGTCTTC CTGGGAAGAA
    CTTGAAAGAG ATTCCTATGG GGGCCAGAGA AATGTTAGTG CTTACTGCCT GATGTATATT
    AACGACAAGC TGCCCCACTT CAATGCAGAG GCGGCCCCAA ATGAATTAGA TCAGATGTCA
    GGAGAAGTAG AAGCCCTGTC TGTTGAACTT AAGCATTACA TTCAGGAAGA TAACTGGAGG
    TTTGAGCAGG AAGTGGAGGA ATGGGAAGAA GAGCAGTCTT GTAAAATTCC TCAGATGGCA
    TCTTCCACCA GCTCAGCATC ACAGGACTTC TCTCCATCTC AAGAATCTTC AGTAGCCTCT
    TCCCACGGGG CTCGGTGCCT GTCCTCTGAG CACGCTGTGA TCGTGAAGGA GCAGACTGCC
    CAGGCGATTG CAAACACAGC ACATGCCTAT GAGAACAGTG GCGTAGAAGC TGCACTGAGT
    GAGCTTAAGG AAGCTGAACC CAAGAAGCCC ATGCCCCCGG AAACAAACCT TGCAGAGCAG
    TCAGAGCAGC CCCCACAGGC TCGTGACGCA GAGTCTGCTG CCCAGCCTAA TGCTGAGGTC
    TCTGAAGTCG AGATTCCCAG TGTGGGAAGG ATTCTGGTTA GATCTGATGC AGATGGATAT
    GATGAGGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC
    CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTTA TTAAGGCATT CCATGAAGAG
    TACTCCAGGC TCTATCAGCT CGCCAAGGAG ACCCCCACCT CTCACAGTGA TCCTCGTCTT
    CAGCACGTGC TCATCTACTT CTTCCAAAAT GAAGCGCCTA AAAGGGTAGT AGAGCGGACC
    CTTCTGGAAC AATTTGCCGA TAAAAACCTT AGCTATGATG AAAGATCGAT CAGTATTATG
    AAGGTGGCTC AAGCAAAACT GAAGGAGATT GGTCCAGATG ACATGAATAT GGAAGAGTAC
    AAGAAGTGGC ATGAAGATTA CAGTTTGTTT CGAAAGGTGT CTGTGTATCT GCTGACAGGC
    CTGGAACTCT ATCAGAAAGG AAAGTACCAA GAAGCGCTTT CCTACCTGGT GTATGCCTAC
    CAAAGTAATG CCACTCTGCT GATGAAGGGG CCCCAGCGGG GCGTAAAGGA GTCGGTGATC
    GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTCTTTGAA
    ACAAATGATG AGCACTCTGT AACAGAGGGT ATTAATGTGA TGAATGAGCT GATCATTCCC
    TGTATTCACC TTATCATTAA TAATGACATC TCCAAGGATG ACCTGGATGC CATTGAGGTC
    ATGAGAAACC ACTGGTGCTC TTACCTTGGG CAGGATATCG CAGAAAATCT GCAGCTGTGC
    TTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATTGT CTTAAAGGAG
    CCTCCAACTA TTCGGCCCAA TTCTCCCTAT GACCTTTGTA GCCGATTTGC AGCTGTCATG
    GAGTCAATTC AAGGAGTGTC AGCTGTGACA GTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222700
    Clone ID Related Accession (Same CDS sequence) XM_024975127.1
    Accession Version XM_024975127.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG
    CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC
    GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC
    ATCCTGGCCA TAGCTAAAGC CCGTCAGACC TTTGACCGAG ATGGGTCTGA AGCAGGGCTT
    ATTAAGGCAT TCCATGAAGA GTACTCCAGG CTCTATCAGC TCGCCAAGGA GACCCCCACC
    TCTCACAGTG ATCCTCGTCT TCAGCACGTG CTCATCTACT TCTTCCAAAA TGAAGCGCCT
    AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA CAATTTGCCG ATAAAAACCT TAGCTATGAT
    GAAAGATCGA TCAGTATTAT GAAGGTGGCT CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT
    GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ACAGTTTGTT TCGAAAGGTG
    TCTGTGTATC TGCTGACAGG CCTGGAACTC TATCAGAAAG GAAAGTACCA AGAAGCGCTT
    TCCTACCTGG TGTATGCCTA CCAAAGTAAT GCCACTCTGC TGATGAAGGG GCCCCAGCGG
    GGCGTAAAGG AGTCGGTGAT CGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC
    AAAGCAGCTT CTCTCTTTGA AACAAATGAT GAGCACTCTG TAACAGAGGG TATTAATGTG
    ATGAATGAGC TGATCATTCC CTGTATTCAC CTTATCATTA ATAATGACAT CTCCAAGGAT
    GACCTGGATG CCATTGAGGT CATGAGAAAC CACTGGTGCT CTTACCTTGG GCAGGATATC
    GCAGAAAATC TGCAGCTGTG CTTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA
    GAAATCATTG TCTTAAAGGA GCCTCCAACT ATTCGGCCCA ATTCTCCCTA TGACCTTTGT
    AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830895.1
    CDS87..3446
    Translation

    Target ORF information:

    RefSeq Version XM_024975127.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975127.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG
    CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC
    GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC
    ATCCTGGCCA TAGCTAAAGC CCGTCAGACC TTTGACCGAG ATGGGTCTGA AGCAGGGCTT
    ATTAAGGCAT TCCATGAAGA GTACTCCAGG CTCTATCAGC TCGCCAAGGA GACCCCCACC
    TCTCACAGTG ATCCTCGTCT TCAGCACGTG CTCATCTACT TCTTCCAAAA TGAAGCGCCT
    AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA CAATTTGCCG ATAAAAACCT TAGCTATGAT
    GAAAGATCGA TCAGTATTAT GAAGGTGGCT CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT
    GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ACAGTTTGTT TCGAAAGGTG
    TCTGTGTATC TGCTGACAGG CCTGGAACTC TATCAGAAAG GAAAGTACCA AGAAGCGCTT
    TCCTACCTGG TGTATGCCTA CCAAAGTAAT GCCACTCTGC TGATGAAGGG GCCCCAGCGG
    GGCGTAAAGG AGTCGGTGAT CGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC
    AAAGCAGCTT CTCTCTTTGA AACAAATGAT GAGCACTCTG TAACAGAGGG TATTAATGTG
    ATGAATGAGC TGATCATTCC CTGTATTCAC CTTATCATTA ATAATGACAT CTCCAAGGAT
    GACCTGGATG CCATTGAGGT CATGAGAAAC CACTGGTGCT CTTACCTTGG GCAGGATATC
    GCAGAAAATC TGCAGCTGTG CTTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA
    GAAATCATTG TCTTAAAGGA GCCTCCAACT ATTCGGCCCA ATTCTCCCTA TGACCTTTGT
    AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222701
    Clone ID Related Accession (Same CDS sequence) XM_024975128.1
    Accession Version XM_024975128.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG
    CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC
    GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA
    AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC
    ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT
    GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG
    GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA
    AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC
    CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG
    ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG
    TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC
    CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG
    GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG
    GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG
    GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC
    ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT
    GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA
    GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC
    TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG
    ACAGTGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830896.1
    CDS87..3218
    Translation

    Target ORF information:

    RefSeq Version XM_024975128.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975128.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG
    CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC
    GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA
    AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC
    ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT
    GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG
    GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA
    AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC
    CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG
    ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG
    TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC
    CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG
    GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG
    GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG
    GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC
    ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT
    GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA
    GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC
    TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG
    ACAGTGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92628
    Clone ID Related Accession (Same CDS sequence) XM_015474609.2 , XM_015474609.1
    Accession Version XM_015474609.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474609.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG
    GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT
    GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG
    TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC
    AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC
    CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA
    AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG
    GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT
    TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG
    TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG
    AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT
    CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA
    GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT
    GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC
    CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT
    CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT
    CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT
    GTGACAGTGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330095.1
    CDS87..3341
    Translation

    Target ORF information:

    RefSeq Version XM_015474609.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474609.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG
    GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT
    GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG
    TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC
    AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC
    CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA
    AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG
    GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT
    TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG
    TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG
    AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT
    CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA
    GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT
    GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC
    CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT
    CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT
    CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT
    GTGACAGTGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92628
    Clone ID Related Accession (Same CDS sequence) XM_015474609.2 , XM_015474609.1
    Accession Version XM_015474609.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG
    GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT
    GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG
    TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC
    AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC
    CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA
    AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG
    GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT
    TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG
    TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG
    AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT
    CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA
    GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT
    GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC
    CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT
    CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT
    CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT
    GTGACAGTGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330095.1
    CDS70..3324
    Translation

    Target ORF information:

    RefSeq Version XM_015474609.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474609.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG
    GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT
    GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG
    TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC
    AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC
    CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA
    AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG
    GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT
    TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG
    TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG
    AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT
    CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA
    GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT
    GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC
    CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT
    CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT
    CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT
    GTGACAGTGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92629
    Clone ID Related Accession (Same CDS sequence) XM_015474610.1 , XM_015474610.2
    Accession Version XM_015474610.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG TGATGCTGAG CCCTGCCATG
    CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC CGTCAGACCT TTGACCGAGA TGGGTCTGAA
    GCAGGGCTTA TTAAGGCATT CCATGAAGAG TACTCCAGGC TCTATCAGCT CGCCAAGGAG
    ACCCCCACCT CTCACAGTGA TCCTCGTCTT CAGCACGTGC TCATCTACTT CTTCCAAAAT
    GAAGCGCCTA AAAGGGTAGT AGAGCGGACC CTTCTGGAAC AATTTGCCGA TAAAAACCTT
    AGCTATGATG AAAGATCGAT CAGTATTATG AAGGTGGCTC AAGCAAAACT GAAGGAGATT
    GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA CAGTTTGTTT
    CGAAAGGTGT CTGTGTATCT GCTGACAGGC CTGGAACTCT ATCAGAAAGG AAAGTACCAA
    GAAGCGCTTT CCTACCTGGT GTATGCCTAC CAAAGTAATG CCACTCTGCT GATGAAGGGG
    CCCCAGCGGG GCGTAAAGGA GTCGGTGATC GCTTTATACC GAAGAAAATG CCTTCTGGAG
    CTGAATGCCA AAGCAGCTTC TCTCTTTGAA ACAAATGATG AGCACTCTGT AACAGAGGGT
    ATTAATGTGA TGAATGAGCT GATCATTCCC TGTATTCACC TTATCATTAA TAATGACATC
    TCCAAGGATG ACCTGGATGC CATTGAGGTC ATGAGAAACC ACTGGTGCTC TTACCTTGGG
    CAGGATATCG CAGAAAATCT GCAGCTGTGC TTAGGGGAGT TTCTACCCAG ACTTCTAGAT
    CCTTCTGCAG AAATCATTGT CTTAAAGGAG CCTCCAACTA TTCGGCCCAA TTCTCCCTAT
    GACCTTTGTA GCCGATTTGC AGCTGTCATG GAGTCAATTC AAGGAGTGTC AGCTGTGACA
    GTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330096.1
    CDS70..3318
    Translation

    Target ORF information:

    RefSeq Version XM_015474610.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474610.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG TGATGCTGAG CCCTGCCATG
    CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC CGTCAGACCT TTGACCGAGA TGGGTCTGAA
    GCAGGGCTTA TTAAGGCATT CCATGAAGAG TACTCCAGGC TCTATCAGCT CGCCAAGGAG
    ACCCCCACCT CTCACAGTGA TCCTCGTCTT CAGCACGTGC TCATCTACTT CTTCCAAAAT
    GAAGCGCCTA AAAGGGTAGT AGAGCGGACC CTTCTGGAAC AATTTGCCGA TAAAAACCTT
    AGCTATGATG AAAGATCGAT CAGTATTATG AAGGTGGCTC AAGCAAAACT GAAGGAGATT
    GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA CAGTTTGTTT
    CGAAAGGTGT CTGTGTATCT GCTGACAGGC CTGGAACTCT ATCAGAAAGG AAAGTACCAA
    GAAGCGCTTT CCTACCTGGT GTATGCCTAC CAAAGTAATG CCACTCTGCT GATGAAGGGG
    CCCCAGCGGG GCGTAAAGGA GTCGGTGATC GCTTTATACC GAAGAAAATG CCTTCTGGAG
    CTGAATGCCA AAGCAGCTTC TCTCTTTGAA ACAAATGATG AGCACTCTGT AACAGAGGGT
    ATTAATGTGA TGAATGAGCT GATCATTCCC TGTATTCACC TTATCATTAA TAATGACATC
    TCCAAGGATG ACCTGGATGC CATTGAGGTC ATGAGAAACC ACTGGTGCTC TTACCTTGGG
    CAGGATATCG CAGAAAATCT GCAGCTGTGC TTAGGGGAGT TTCTACCCAG ACTTCTAGAT
    CCTTCTGCAG AAATCATTGT CTTAAAGGAG CCTCCAACTA TTCGGCCCAA TTCTCCCTAT
    GACCTTTGTA GCCGATTTGC AGCTGTCATG GAGTCAATTC AAGGAGTGTC AGCTGTGACA
    GTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92629
    Clone ID Related Accession (Same CDS sequence) XM_015474610.1 , XM_015474610.2
    Accession Version XM_015474610.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG TGATGCTGAG CCCTGCCATG
    CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC CGTCAGACCT TTGACCGAGA TGGGTCTGAA
    GCAGGGCTTA TTAAGGCATT CCATGAAGAG TACTCCAGGC TCTATCAGCT CGCCAAGGAG
    ACCCCCACCT CTCACAGTGA TCCTCGTCTT CAGCACGTGC TCATCTACTT CTTCCAAAAT
    GAAGCGCCTA AAAGGGTAGT AGAGCGGACC CTTCTGGAAC AATTTGCCGA TAAAAACCTT
    AGCTATGATG AAAGATCGAT CAGTATTATG AAGGTGGCTC AAGCAAAACT GAAGGAGATT
    GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA CAGTTTGTTT
    CGAAAGGTGT CTGTGTATCT GCTGACAGGC CTGGAACTCT ATCAGAAAGG AAAGTACCAA
    GAAGCGCTTT CCTACCTGGT GTATGCCTAC CAAAGTAATG CCACTCTGCT GATGAAGGGG
    CCCCAGCGGG GCGTAAAGGA GTCGGTGATC GCTTTATACC GAAGAAAATG CCTTCTGGAG
    CTGAATGCCA AAGCAGCTTC TCTCTTTGAA ACAAATGATG AGCACTCTGT AACAGAGGGT
    ATTAATGTGA TGAATGAGCT GATCATTCCC TGTATTCACC TTATCATTAA TAATGACATC
    TCCAAGGATG ACCTGGATGC CATTGAGGTC ATGAGAAACC ACTGGTGCTC TTACCTTGGG
    CAGGATATCG CAGAAAATCT GCAGCTGTGC TTAGGGGAGT TTCTACCCAG ACTTCTAGAT
    CCTTCTGCAG AAATCATTGT CTTAAAGGAG CCTCCAACTA TTCGGCCCAA TTCTCCCTAT
    GACCTTTGTA GCCGATTTGC AGCTGTCATG GAGTCAATTC AAGGAGTGTC AGCTGTGACA
    GTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330096.1
    CDS87..3335
    Translation

    Target ORF information:

    RefSeq Version XM_015474610.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474610.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG TGATGCTGAG CCCTGCCATG
    CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC CGTCAGACCT TTGACCGAGA TGGGTCTGAA
    GCAGGGCTTA TTAAGGCATT CCATGAAGAG TACTCCAGGC TCTATCAGCT CGCCAAGGAG
    ACCCCCACCT CTCACAGTGA TCCTCGTCTT CAGCACGTGC TCATCTACTT CTTCCAAAAT
    GAAGCGCCTA AAAGGGTAGT AGAGCGGACC CTTCTGGAAC AATTTGCCGA TAAAAACCTT
    AGCTATGATG AAAGATCGAT CAGTATTATG AAGGTGGCTC AAGCAAAACT GAAGGAGATT
    GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA CAGTTTGTTT
    CGAAAGGTGT CTGTGTATCT GCTGACAGGC CTGGAACTCT ATCAGAAAGG AAAGTACCAA
    GAAGCGCTTT CCTACCTGGT GTATGCCTAC CAAAGTAATG CCACTCTGCT GATGAAGGGG
    CCCCAGCGGG GCGTAAAGGA GTCGGTGATC GCTTTATACC GAAGAAAATG CCTTCTGGAG
    CTGAATGCCA AAGCAGCTTC TCTCTTTGAA ACAAATGATG AGCACTCTGT AACAGAGGGT
    ATTAATGTGA TGAATGAGCT GATCATTCCC TGTATTCACC TTATCATTAA TAATGACATC
    TCCAAGGATG ACCTGGATGC CATTGAGGTC ATGAGAAACC ACTGGTGCTC TTACCTTGGG
    CAGGATATCG CAGAAAATCT GCAGCTGTGC TTAGGGGAGT TTCTACCCAG ACTTCTAGAT
    CCTTCTGCAG AAATCATTGT CTTAAAGGAG CCTCCAACTA TTCGGCCCAA TTCTCCCTAT
    GACCTTTGTA GCCGATTTGC AGCTGTCATG GAGTCAATTC AAGGAGTGTC AGCTGTGACA
    GTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40337
    Clone ID Related Accession (Same CDS sequence) NM_001192998.1
    Accession Version NM_001192998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On Jul 17, 2010 this sequence version replaced XM_865093.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGTGA TGCTGAGCCC TGCCATGCAA
    GGGGTCATCC TGGCCATAGC TAAAGCCCGT CAGACCTTTG ACCGAGATGG GTCTGAAGCA
    GGGCTTATTA AGGCATTCCA TGAAGAGTAC TCCAGGCTCT ATCAGCTCGC CAAGGAGACC
    CCCACCTCTC ACAGTGATCC TCGTCTTCAG CACGTGCTCA TCTACTTCTT CCAAAATGAA
    GCGCCTAAAA GGGTAGTAGA GCGGACCCTT CTGGAACAAT TTGCCGATAA AAACCTTAGC
    TATGATGAAA GATCGATCAG TATTATGAAG GTGGCTCAAG CAAAACTGAA GGAGATTGGT
    CCAGATGACA TGAATATGGA AGAGTACAAG AAGTGGCATG AAGATTACAG TTTGTTTCGA
    AAGGTGTCTG TGTATCTGCT GACAGGCCTG GAACTCTATC AGAAAGGAAA GTACCAAGAA
    GCGCTTTCCT ACCTGGTGTA TGCCTACCAA AGTAATGCCA CTCTGCTGAT GAAGGGGCCC
    CAGCGGGGCG TAAAGGAGTC GGTGATCGCT TTATACCGAA GAAAATGCCT TCTGGAGCTG
    AATGCCAAAG CAGCTTCTCT CTTTGAAACA AATGATGAGC ACTCTGTAAC AGAGGGTATT
    AATGTGATGA ATGAGCTGAT CATTCCCTGT ATTCACCTTA TCATTAATAA TGACATCTCC
    AAGGATGACC TGGATGCCAT TGAGGTCATG AGAAACCACT GGTGCTCTTA CCTTGGGCAG
    GATATCGCAG AAAATCTGCA GCTGTGCTTA GGGGAGTTTC TACCCAGACT TCTAGATCCT
    TCTGCAGAAA TCATTGTCTT AAAGGAGCCT CCAACTATTC GGCCCAATTC TCCCTATGAC
    CTTTGTAGCC GATTTGCAGC TGTCATGGAG TCAATTCAAG GAGTGTCAGC TGTGACAGTG
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179927.1
    CDS32..3277
    Translation

    Target ORF information:

    RefSeq Version NM_001192998.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGTGA TGCTGAGCCC TGCCATGCAA
    GGGGTCATCC TGGCCATAGC TAAAGCCCGT CAGACCTTTG ACCGAGATGG GTCTGAAGCA
    GGGCTTATTA AGGCATTCCA TGAAGAGTAC TCCAGGCTCT ATCAGCTCGC CAAGGAGACC
    CCCACCTCTC ACAGTGATCC TCGTCTTCAG CACGTGCTCA TCTACTTCTT CCAAAATGAA
    GCGCCTAAAA GGGTAGTAGA GCGGACCCTT CTGGAACAAT TTGCCGATAA AAACCTTAGC
    TATGATGAAA GATCGATCAG TATTATGAAG GTGGCTCAAG CAAAACTGAA GGAGATTGGT
    CCAGATGACA TGAATATGGA AGAGTACAAG AAGTGGCATG AAGATTACAG TTTGTTTCGA
    AAGGTGTCTG TGTATCTGCT GACAGGCCTG GAACTCTATC AGAAAGGAAA GTACCAAGAA
    GCGCTTTCCT ACCTGGTGTA TGCCTACCAA AGTAATGCCA CTCTGCTGAT GAAGGGGCCC
    CAGCGGGGCG TAAAGGAGTC GGTGATCGCT TTATACCGAA GAAAATGCCT TCTGGAGCTG
    AATGCCAAAG CAGCTTCTCT CTTTGAAACA AATGATGAGC ACTCTGTAAC AGAGGGTATT
    AATGTGATGA ATGAGCTGAT CATTCCCTGT ATTCACCTTA TCATTAATAA TGACATCTCC
    AAGGATGACC TGGATGCCAT TGAGGTCATG AGAAACCACT GGTGCTCTTA CCTTGGGCAG
    GATATCGCAG AAAATCTGCA GCTGTGCTTA GGGGAGTTTC TACCCAGACT TCTAGATCCT
    TCTGCAGAAA TCATTGTCTT AAAGGAGCCT CCAACTATTC GGCCCAATTC TCCCTATGAC
    CTTTGTAGCC GATTTGCAGC TGTCATGGAG TCAATTCAAG GAGTGTCAGC TGTGACAGTG
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26216
    Clone ID Related Accession (Same CDS sequence) XM_005215811.4 , XM_005215811.3
    Accession Version XM_005215811.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215811.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG
    GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT
    GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG
    TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC
    AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC
    CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA
    AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG
    GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT
    TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG
    TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG
    AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT
    CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA
    GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT
    GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC
    CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT
    CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT
    CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT
    GTGACAGTGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215868.1
    CDS87..3521
    Translation

    Target ORF information:

    RefSeq Version XM_005215811.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215811.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG
    GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT
    GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG
    TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC
    AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC
    CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA
    AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG
    GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT
    TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG
    TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG
    AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT
    CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA
    GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT
    GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC
    CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT
    CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT
    CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT
    GTGACAGTGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26216
    Clone ID Related Accession (Same CDS sequence) XM_005215811.4 , XM_005215811.3
    Accession Version XM_005215811.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG
    GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT
    GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG
    TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC
    AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC
    CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA
    AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG
    GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT
    TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG
    TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG
    AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT
    CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA
    GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT
    GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC
    CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT
    CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT
    CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT
    GTGACAGTGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215868.1
    CDS70..3504
    Translation

    Target ORF information:

    RefSeq Version XM_005215811.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215811.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG
    GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT
    GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG
    TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC
    AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC
    CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA
    AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG
    GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT
    TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG
    TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG
    AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT
    CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA
    GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT
    GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC
    CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT
    CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT
    CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT
    GTGACAGTGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26217
    Clone ID Related Accession (Same CDS sequence) XM_005215812.4 , XM_005215812.3
    Accession Version XM_005215812.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215812.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG
    CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC
    GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA
    AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC
    ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT
    GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG
    GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA
    AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC
    CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG
    ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG
    TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC
    CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG
    GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG
    GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG
    GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC
    ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT
    GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA
    GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC
    TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG
    ACAGTGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215869.1
    CDS87..3518
    Translation

    Target ORF information:

    RefSeq Version XM_005215812.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215812.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG
    CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC
    GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA
    AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC
    ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT
    GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG
    GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA
    AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC
    CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG
    ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG
    TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC
    CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG
    GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG
    GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG
    GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC
    ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT
    GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA
    GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC
    TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG
    ACAGTGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26217
    Clone ID Related Accession (Same CDS sequence) XM_005215812.4 , XM_005215812.3
    Accession Version XM_005215812.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG
    CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC
    GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA
    AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC
    ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT
    GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG
    GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA
    AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC
    CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG
    ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG
    TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC
    CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG
    GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG
    GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG
    GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC
    ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT
    GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA
    GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC
    TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG
    ACAGTGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215869.1
    CDS70..3501
    Translation

    Target ORF information:

    RefSeq Version XM_005215812.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215812.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG
    CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC
    GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA
    AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC
    ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT
    GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG
    GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA
    AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC
    CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG
    ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG
    TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC
    CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG
    GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG
    GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG
    GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC
    ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT
    GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA
    GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC
    TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG
    ACAGTGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26219
    Clone ID Related Accession (Same CDS sequence) XM_005215814.4 , XM_005215814.3
    Accession Version XM_005215814.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215814.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTGATGC TGAGCCCTGC CATGCAAGGG
    GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG
    CTTATTAAGG CATTCCATGA AGAGTACTCC AGGCTCTATC AGCTCGCCAA GGAGACCCCC
    ACCTCTCACA GTGATCCTCG TCTTCAGCAC GTGCTCATCT ACTTCTTCCA AAATGAAGCG
    CCTAAAAGGG TAGTAGAGCG GACCCTTCTG GAACAATTTG CCGATAAAAA CCTTAGCTAT
    GATGAAAGAT CGATCAGTAT TATGAAGGTG GCTCAAGCAA AACTGAAGGA GATTGGTCCA
    GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTACAGTTT GTTTCGAAAG
    GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA CTCTATCAGA AAGGAAAGTA CCAAGAAGCG
    CTTTCCTACC TGGTGTATGC CTACCAAAGT AATGCCACTC TGCTGATGAA GGGGCCCCAG
    CGGGGCGTAA AGGAGTCGGT GATCGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT
    GCCAAAGCAG CTTCTCTCTT TGAAACAAAT GATGAGCACT CTGTAACAGA GGGTATTAAT
    GTGATGAATG AGCTGATCAT TCCCTGTATT CACCTTATCA TTAATAATGA CATCTCCAAG
    GATGACCTGG ATGCCATTGA GGTCATGAGA AACCACTGGT GCTCTTACCT TGGGCAGGAT
    ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT
    GCAGAAATCA TTGTCTTAAA GGAGCCTCCA ACTATTCGGC CCAATTCTCC CTATGACCTT
    TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAAGGAG TGTCAGCTGT GACAGTGAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215871.1
    CDS87..3449
    Translation

    Target ORF information:

    RefSeq Version XM_005215814.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215814.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTGATGC TGAGCCCTGC CATGCAAGGG
    GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG
    CTTATTAAGG CATTCCATGA AGAGTACTCC AGGCTCTATC AGCTCGCCAA GGAGACCCCC
    ACCTCTCACA GTGATCCTCG TCTTCAGCAC GTGCTCATCT ACTTCTTCCA AAATGAAGCG
    CCTAAAAGGG TAGTAGAGCG GACCCTTCTG GAACAATTTG CCGATAAAAA CCTTAGCTAT
    GATGAAAGAT CGATCAGTAT TATGAAGGTG GCTCAAGCAA AACTGAAGGA GATTGGTCCA
    GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTACAGTTT GTTTCGAAAG
    GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA CTCTATCAGA AAGGAAAGTA CCAAGAAGCG
    CTTTCCTACC TGGTGTATGC CTACCAAAGT AATGCCACTC TGCTGATGAA GGGGCCCCAG
    CGGGGCGTAA AGGAGTCGGT GATCGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT
    GCCAAAGCAG CTTCTCTCTT TGAAACAAAT GATGAGCACT CTGTAACAGA GGGTATTAAT
    GTGATGAATG AGCTGATCAT TCCCTGTATT CACCTTATCA TTAATAATGA CATCTCCAAG
    GATGACCTGG ATGCCATTGA GGTCATGAGA AACCACTGGT GCTCTTACCT TGGGCAGGAT
    ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT
    GCAGAAATCA TTGTCTTAAA GGAGCCTCCA ACTATTCGGC CCAATTCTCC CTATGACCTT
    TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAAGGAG TGTCAGCTGT GACAGTGAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26219
    Clone ID Related Accession (Same CDS sequence) XM_005215814.4 , XM_005215814.3
    Accession Version XM_005215814.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215814.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTGATGC TGAGCCCTGC CATGCAAGGG
    GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG
    CTTATTAAGG CATTCCATGA AGAGTACTCC AGGCTCTATC AGCTCGCCAA GGAGACCCCC
    ACCTCTCACA GTGATCCTCG TCTTCAGCAC GTGCTCATCT ACTTCTTCCA AAATGAAGCG
    CCTAAAAGGG TAGTAGAGCG GACCCTTCTG GAACAATTTG CCGATAAAAA CCTTAGCTAT
    GATGAAAGAT CGATCAGTAT TATGAAGGTG GCTCAAGCAA AACTGAAGGA GATTGGTCCA
    GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTACAGTTT GTTTCGAAAG
    GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA CTCTATCAGA AAGGAAAGTA CCAAGAAGCG
    CTTTCCTACC TGGTGTATGC CTACCAAAGT AATGCCACTC TGCTGATGAA GGGGCCCCAG
    CGGGGCGTAA AGGAGTCGGT GATCGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT
    GCCAAAGCAG CTTCTCTCTT TGAAACAAAT GATGAGCACT CTGTAACAGA GGGTATTAAT
    GTGATGAATG AGCTGATCAT TCCCTGTATT CACCTTATCA TTAATAATGA CATCTCCAAG
    GATGACCTGG ATGCCATTGA GGTCATGAGA AACCACTGGT GCTCTTACCT TGGGCAGGAT
    ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT
    GCAGAAATCA TTGTCTTAAA GGAGCCTCCA ACTATTCGGC CCAATTCTCC CTATGACCTT
    TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAAGGAG TGTCAGCTGT GACAGTGAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215871.1
    CDS70..3432
    Translation

    Target ORF information:

    RefSeq Version XM_005215814.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215814.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG
    AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT
    GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTGATGC TGAGCCCTGC CATGCAAGGG
    GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG
    CTTATTAAGG CATTCCATGA AGAGTACTCC AGGCTCTATC AGCTCGCCAA GGAGACCCCC
    ACCTCTCACA GTGATCCTCG TCTTCAGCAC GTGCTCATCT ACTTCTTCCA AAATGAAGCG
    CCTAAAAGGG TAGTAGAGCG GACCCTTCTG GAACAATTTG CCGATAAAAA CCTTAGCTAT
    GATGAAAGAT CGATCAGTAT TATGAAGGTG GCTCAAGCAA AACTGAAGGA GATTGGTCCA
    GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTACAGTTT GTTTCGAAAG
    GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA CTCTATCAGA AAGGAAAGTA CCAAGAAGCG
    CTTTCCTACC TGGTGTATGC CTACCAAAGT AATGCCACTC TGCTGATGAA GGGGCCCCAG
    CGGGGCGTAA AGGAGTCGGT GATCGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT
    GCCAAAGCAG CTTCTCTCTT TGAAACAAAT GATGAGCACT CTGTAACAGA GGGTATTAAT
    GTGATGAATG AGCTGATCAT TCCCTGTATT CACCTTATCA TTAATAATGA CATCTCCAAG
    GATGACCTGG ATGCCATTGA GGTCATGAGA AACCACTGGT GCTCTTACCT TGGGCAGGAT
    ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT
    GCAGAAATCA TTGTCTTAAA GGAGCCTCCA ACTATTCGGC CCAATTCTCC CTATGACCTT
    TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAAGGAG TGTCAGCTGT GACAGTGAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26221
    Clone ID Related Accession (Same CDS sequence) XM_005215816.3 , XM_005215816.4
    Accession Version XM_005215816.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG CATTCCATGA AGAGTACTCC
    AGGCTCTATC AGCTCGCCAA GGAGACCCCC ACCTCTCACA GTGATCCTCG TCTTCAGCAC
    GTGCTCATCT ACTTCTTCCA AAATGAAGCG CCTAAAAGGG TAGTAGAGCG GACCCTTCTG
    GAACAATTTG CCGATAAAAA CCTTAGCTAT GATGAAAGAT CGATCAGTAT TATGAAGGTG
    GCTCAAGCAA AACTGAAGGA GATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG
    TGGCATGAAG ATTACAGTTT GTTTCGAAAG GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA
    CTCTATCAGA AAGGAAAGTA CCAAGAAGCG CTTTCCTACC TGGTGTATGC CTACCAAAGT
    AATGCCACTC TGCTGATGAA GGGGCCCCAG CGGGGCGTAA AGGAGTCGGT GATCGCTTTA
    TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTCTT TGAAACAAAT
    GATGAGCACT CTGTAACAGA GGGTATTAAT GTGATGAATG AGCTGATCAT TCCCTGTATT
    CACCTTATCA TTAATAATGA CATCTCCAAG GATGACCTGG ATGCCATTGA GGTCATGAGA
    AACCACTGGT GCTCTTACCT TGGGCAGGAT ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG
    GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TTGTCTTAAA GGAGCCTCCA
    ACTATTCGGC CCAATTCTCC CTATGACCTT TGTAGCCGAT TTGCAGCTGT CATGGAGTCA
    ATTCAAGGAG TGTCAGCTGT GACAGTGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215873.1
    CDS70..3222
    Translation

    Target ORF information:

    RefSeq Version XM_005215816.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215816.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG CATTCCATGA AGAGTACTCC
    AGGCTCTATC AGCTCGCCAA GGAGACCCCC ACCTCTCACA GTGATCCTCG TCTTCAGCAC
    GTGCTCATCT ACTTCTTCCA AAATGAAGCG CCTAAAAGGG TAGTAGAGCG GACCCTTCTG
    GAACAATTTG CCGATAAAAA CCTTAGCTAT GATGAAAGAT CGATCAGTAT TATGAAGGTG
    GCTCAAGCAA AACTGAAGGA GATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG
    TGGCATGAAG ATTACAGTTT GTTTCGAAAG GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA
    CTCTATCAGA AAGGAAAGTA CCAAGAAGCG CTTTCCTACC TGGTGTATGC CTACCAAAGT
    AATGCCACTC TGCTGATGAA GGGGCCCCAG CGGGGCGTAA AGGAGTCGGT GATCGCTTTA
    TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTCTT TGAAACAAAT
    GATGAGCACT CTGTAACAGA GGGTATTAAT GTGATGAATG AGCTGATCAT TCCCTGTATT
    CACCTTATCA TTAATAATGA CATCTCCAAG GATGACCTGG ATGCCATTGA GGTCATGAGA
    AACCACTGGT GCTCTTACCT TGGGCAGGAT ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG
    GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TTGTCTTAAA GGAGCCTCCA
    ACTATTCGGC CCAATTCTCC CTATGACCTT TGTAGCCGAT TTGCAGCTGT CATGGAGTCA
    ATTCAAGGAG TGTCAGCTGT GACAGTGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26221
    Clone ID Related Accession (Same CDS sequence) XM_005215816.3 , XM_005215816.4
    Accession Version XM_005215816.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215816.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG CATTCCATGA AGAGTACTCC
    AGGCTCTATC AGCTCGCCAA GGAGACCCCC ACCTCTCACA GTGATCCTCG TCTTCAGCAC
    GTGCTCATCT ACTTCTTCCA AAATGAAGCG CCTAAAAGGG TAGTAGAGCG GACCCTTCTG
    GAACAATTTG CCGATAAAAA CCTTAGCTAT GATGAAAGAT CGATCAGTAT TATGAAGGTG
    GCTCAAGCAA AACTGAAGGA GATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG
    TGGCATGAAG ATTACAGTTT GTTTCGAAAG GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA
    CTCTATCAGA AAGGAAAGTA CCAAGAAGCG CTTTCCTACC TGGTGTATGC CTACCAAAGT
    AATGCCACTC TGCTGATGAA GGGGCCCCAG CGGGGCGTAA AGGAGTCGGT GATCGCTTTA
    TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTCTT TGAAACAAAT
    GATGAGCACT CTGTAACAGA GGGTATTAAT GTGATGAATG AGCTGATCAT TCCCTGTATT
    CACCTTATCA TTAATAATGA CATCTCCAAG GATGACCTGG ATGCCATTGA GGTCATGAGA
    AACCACTGGT GCTCTTACCT TGGGCAGGAT ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG
    GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TTGTCTTAAA GGAGCCTCCA
    ACTATTCGGC CCAATTCTCC CTATGACCTT TGTAGCCGAT TTGCAGCTGT CATGGAGTCA
    ATTCAAGGAG TGTCAGCTGT GACAGTGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215873.1
    CDS87..3239
    Translation

    Target ORF information:

    RefSeq Version XM_005215816.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215816.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC
    AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG
    ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA
    AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG
    CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT
    CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG
    AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG
    AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA
    ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG
    CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC
    TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT
    TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA
    ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA
    CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG
    GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA
    CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT
    CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA
    AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC
    AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC
    CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT
    GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT
    GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG
    GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG
    GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC
    TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT
    GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG CATTCCATGA AGAGTACTCC
    AGGCTCTATC AGCTCGCCAA GGAGACCCCC ACCTCTCACA GTGATCCTCG TCTTCAGCAC
    GTGCTCATCT ACTTCTTCCA AAATGAAGCG CCTAAAAGGG TAGTAGAGCG GACCCTTCTG
    GAACAATTTG CCGATAAAAA CCTTAGCTAT GATGAAAGAT CGATCAGTAT TATGAAGGTG
    GCTCAAGCAA AACTGAAGGA GATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG
    TGGCATGAAG ATTACAGTTT GTTTCGAAAG GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA
    CTCTATCAGA AAGGAAAGTA CCAAGAAGCG CTTTCCTACC TGGTGTATGC CTACCAAAGT
    AATGCCACTC TGCTGATGAA GGGGCCCCAG CGGGGCGTAA AGGAGTCGGT GATCGCTTTA
    TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTCTT TGAAACAAAT
    GATGAGCACT CTGTAACAGA GGGTATTAAT GTGATGAATG AGCTGATCAT TCCCTGTATT
    CACCTTATCA TTAATAATGA CATCTCCAAG GATGACCTGG ATGCCATTGA GGTCATGAGA
    AACCACTGGT GCTCTTACCT TGGGCAGGAT ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG
    GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TTGTCTTAAA GGAGCCTCCA
    ACTATTCGGC CCAATTCTCC CTATGACCTT TGTAGCCGAT TTGCAGCTGT CATGGAGTCA
    ATTCAAGGAG TGTCAGCTGT GACAGTGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26222
    Clone ID Related Accession (Same CDS sequence) XM_005215817.3 , XM_005215817.4
    Accession Version XM_005215817.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215817.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGCAT TCCATGAAGA GTACTCCAGG
    CTCTATCAGC TCGCCAAGGA GACCCCCACC TCTCACAGTG ATCCTCGTCT TCAGCACGTG
    CTCATCTACT TCTTCCAAAA TGAAGCGCCT AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA
    CAATTTGCCG ATAAAAACCT TAGCTATGAT GAAAGATCGA TCAGTATTAT GAAGGTGGCT
    CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT GACATGAATA TGGAAGAGTA CAAGAAGTGG
    CATGAAGATT ACAGTTTGTT TCGAAAGGTG TCTGTGTATC TGCTGACAGG CCTGGAACTC
    TATCAGAAAG GAAAGTACCA AGAAGCGCTT TCCTACCTGG TGTATGCCTA CCAAAGTAAT
    GCCACTCTGC TGATGAAGGG GCCCCAGCGG GGCGTAAAGG AGTCGGTGAT CGCTTTATAC
    CGAAGAAAAT GCCTTCTGGA GCTGAATGCC AAAGCAGCTT CTCTCTTTGA AACAAATGAT
    GAGCACTCTG TAACAGAGGG TATTAATGTG ATGAATGAGC TGATCATTCC CTGTATTCAC
    CTTATCATTA ATAATGACAT CTCCAAGGAT GACCTGGATG CCATTGAGGT CATGAGAAAC
    CACTGGTGCT CTTACCTTGG GCAGGATATC GCAGAAAATC TGCAGCTGTG CTTAGGGGAG
    TTTCTACCCA GACTTCTAGA TCCTTCTGCA GAAATCATTG TCTTAAAGGA GCCTCCAACT
    ATTCGGCCCA ATTCTCCCTA TGACCTTTGT AGCCGATTTG CAGCTGTCAT GGAGTCAATT
    CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215874.1
    CDS70..3219
    Translation

    Target ORF information:

    RefSeq Version XM_005215817.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215817.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGCAT TCCATGAAGA GTACTCCAGG
    CTCTATCAGC TCGCCAAGGA GACCCCCACC TCTCACAGTG ATCCTCGTCT TCAGCACGTG
    CTCATCTACT TCTTCCAAAA TGAAGCGCCT AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA
    CAATTTGCCG ATAAAAACCT TAGCTATGAT GAAAGATCGA TCAGTATTAT GAAGGTGGCT
    CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT GACATGAATA TGGAAGAGTA CAAGAAGTGG
    CATGAAGATT ACAGTTTGTT TCGAAAGGTG TCTGTGTATC TGCTGACAGG CCTGGAACTC
    TATCAGAAAG GAAAGTACCA AGAAGCGCTT TCCTACCTGG TGTATGCCTA CCAAAGTAAT
    GCCACTCTGC TGATGAAGGG GCCCCAGCGG GGCGTAAAGG AGTCGGTGAT CGCTTTATAC
    CGAAGAAAAT GCCTTCTGGA GCTGAATGCC AAAGCAGCTT CTCTCTTTGA AACAAATGAT
    GAGCACTCTG TAACAGAGGG TATTAATGTG ATGAATGAGC TGATCATTCC CTGTATTCAC
    CTTATCATTA ATAATGACAT CTCCAAGGAT GACCTGGATG CCATTGAGGT CATGAGAAAC
    CACTGGTGCT CTTACCTTGG GCAGGATATC GCAGAAAATC TGCAGCTGTG CTTAGGGGAG
    TTTCTACCCA GACTTCTAGA TCCTTCTGCA GAAATCATTG TCTTAAAGGA GCCTCCAACT
    ATTCGGCCCA ATTCTCCCTA TGACCTTTGT AGCCGATTTG CAGCTGTCAT GGAGTCAATT
    CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26222
    Clone ID Related Accession (Same CDS sequence) XM_005215817.3 , XM_005215817.4
    Accession Version XM_005215817.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215817.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGCAT TCCATGAAGA GTACTCCAGG
    CTCTATCAGC TCGCCAAGGA GACCCCCACC TCTCACAGTG ATCCTCGTCT TCAGCACGTG
    CTCATCTACT TCTTCCAAAA TGAAGCGCCT AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA
    CAATTTGCCG ATAAAAACCT TAGCTATGAT GAAAGATCGA TCAGTATTAT GAAGGTGGCT
    CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT GACATGAATA TGGAAGAGTA CAAGAAGTGG
    CATGAAGATT ACAGTTTGTT TCGAAAGGTG TCTGTGTATC TGCTGACAGG CCTGGAACTC
    TATCAGAAAG GAAAGTACCA AGAAGCGCTT TCCTACCTGG TGTATGCCTA CCAAAGTAAT
    GCCACTCTGC TGATGAAGGG GCCCCAGCGG GGCGTAAAGG AGTCGGTGAT CGCTTTATAC
    CGAAGAAAAT GCCTTCTGGA GCTGAATGCC AAAGCAGCTT CTCTCTTTGA AACAAATGAT
    GAGCACTCTG TAACAGAGGG TATTAATGTG ATGAATGAGC TGATCATTCC CTGTATTCAC
    CTTATCATTA ATAATGACAT CTCCAAGGAT GACCTGGATG CCATTGAGGT CATGAGAAAC
    CACTGGTGCT CTTACCTTGG GCAGGATATC GCAGAAAATC TGCAGCTGTG CTTAGGGGAG
    TTTCTACCCA GACTTCTAGA TCCTTCTGCA GAAATCATTG TCTTAAAGGA GCCTCCAACT
    ATTCGGCCCA ATTCTCCCTA TGACCTTTGT AGCCGATTTG CAGCTGTCAT GGAGTCAATT
    CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215874.1
    CDS87..3236
    Translation

    Target ORF information:

    RefSeq Version XM_005215817.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215817.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG
    TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC
    TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC
    AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG
    AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT
    CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC
    GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG
    CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA
    GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA
    ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC
    AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT
    CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT
    CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT
    GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT
    GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC
    TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT
    CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG
    ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA
    GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT
    CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC
    GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC
    GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG
    AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA
    GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA
    CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA
    GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC
    TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT
    GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC
    TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGCAT TCCATGAAGA GTACTCCAGG
    CTCTATCAGC TCGCCAAGGA GACCCCCACC TCTCACAGTG ATCCTCGTCT TCAGCACGTG
    CTCATCTACT TCTTCCAAAA TGAAGCGCCT AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA
    CAATTTGCCG ATAAAAACCT TAGCTATGAT GAAAGATCGA TCAGTATTAT GAAGGTGGCT
    CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT GACATGAATA TGGAAGAGTA CAAGAAGTGG
    CATGAAGATT ACAGTTTGTT TCGAAAGGTG TCTGTGTATC TGCTGACAGG CCTGGAACTC
    TATCAGAAAG GAAAGTACCA AGAAGCGCTT TCCTACCTGG TGTATGCCTA CCAAAGTAAT
    GCCACTCTGC TGATGAAGGG GCCCCAGCGG GGCGTAAAGG AGTCGGTGAT CGCTTTATAC
    CGAAGAAAAT GCCTTCTGGA GCTGAATGCC AAAGCAGCTT CTCTCTTTGA AACAAATGAT
    GAGCACTCTG TAACAGAGGG TATTAATGTG ATGAATGAGC TGATCATTCC CTGTATTCAC
    CTTATCATTA ATAATGACAT CTCCAAGGAT GACCTGGATG CCATTGAGGT CATGAGAAAC
    CACTGGTGCT CTTACCTTGG GCAGGATATC GCAGAAAATC TGCAGCTGTG CTTAGGGGAG
    TTTCTACCCA GACTTCTAGA TCCTTCTGCA GAAATCATTG TCTTAAAGGA GCCTCCAACT
    ATTCGGCCCA ATTCTCCCTA TGACCTTTGT AGCCGATTTG CAGCTGTCAT GGAGTCAATT
    CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47296
    Clone ID Related Accession (Same CDS sequence) XM_010812360.2
    Accession Version XM_010812360.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGCA AGATGTGAGT GAGTTCACAC ACAAGCTCCT GGATTGGCTG
    GAGGACGCCT TCCAGCTAGC TGTTAATGTT AATAGCAATC CCAGGAACAA ATCTGAAAAT
    CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTCACGGAAG GGATTCGTGA AGGAAAGCCC
    TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCCCTTCAGG TAAACGGTTA TCGTAACTTA
    GACGAATGTT TAGAAGGGGC CATGGTGGAG GGTGACATTG AGCTGCTTCC TTCCGATCAT
    TCAGTGAAGT ACGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA
    CTCTCAAGAT TTGAGTTCAA TCAGTCCCTG GGTCAGCCAG AGAAAATTCA CAATAAGCTT
    GAATTTCCTC AGATCATTTA TATGGACAGG TATATGTACA GGAGCAAAGA GCTTGTTCGA
    AGTAAGCGAG ATTGTATTCG AAAACTCAAA GAAGAAATAA AAATTCTGCA GCAAAAACTG
    GAAAGGTATG TGAAGTATGG CTCGGGCCCA GCCCGGTTCC CGCTTCCGGA CATGTTGAAA
    TACGTGATTG AATTTGCTAG TACAAAACCT GCCTCAGAGA GCTCTGCATC TCAAAGTGAT
    GCGTGCATGA CAGAACCACT TTCTTCAGCA CACTGCCTGA CTTCTGATCT GACATCCAAG
    GAAAGTACAA GTGAAGAGAG CACGTCTCAG GAAGCTGAAG GAACCTTTTC TTCCTCTGAA
    GATTCTGGAC GCAAGTCTAG GATGGCAGAG CAGCCACTTA AACCTCCCCG TTCTTCCGTG
    GAAATGCCCG CACACCCAGC TCCTCGAACA GTCACAGATG AGGAGATAAA CTTTGTTAAG
    ACCTGCCTGC AGAGGTGGAG GAACGAGATT GAACAGGATA TACAAGACTT AAAGAACAGT
    ATTGCAAGCA CTACCCAGAC TATTGAGCAG ATGTACTGTG ATCCTCTCCT CCGTCAGGTG
    CCTTATCGCT TGCACGCGGT CCTCGTTCAC GAAGGACAGG CAAACGCGGG GCACTACTGG
    GCCTACGTCT ATAATCAGCC GCGACAAGTC TGGCTCAAGT ACAACGACAT CTCTGTTACT
    GAGTCTTCCT GGGAAGAACT TGAAAGAGAT TCCTATGGGG GCCAGAGAAA TGTTAGTGCT
    TACTGCCTGA TGTATATTAA CGACAAGCTG CCCCACTTCA ATGCAGAGGC GGCCCCAAAT
    GAATTAGATC AGATGTCAGG AGAAGTAGAA GCCCTGTCTG TTGAACTTAA GCATTACATT
    CAGGAAGATA ACTGGAGGTT TGAGCAGGAA GTGGAGGAAT GGGAAGAAGA GCAGTCTTGT
    AAAATTCCTC AGATGGCATC TTCCACCAGC TCAGCATCAC AGGACTTCTC TCCATCTCAA
    GAATCTTCAG TAGCCTCTTC CCACGGGGCT CGGTGCCTGT CCTCTGAGCA CGCTGTGATC
    GTGAAGGAGC AGACTGCCCA GGCGATTGCA AACACAGCAC ATGCCTATGA GAACAGTGGC
    GTAGAAGCTG CACTGAGTGA GCTTAAGGAA GCTGAACCCA AGAAGCCCAT GCCCCCGGAA
    ACAAACCTTG CAGAGCAGTC AGAGCAGCCC CCACAGGCTC GTGACGCAGA GTCTGCTGCC
    CAGCCTAATG CTGAGGTCTC TGAAGTCGAG ATTCCCAGTG TGGGAAGGAT TCTGGTTAGA
    TCTGATGCAG ATGGATATGA TGAGGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC
    CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTTATT
    AAGGCATTCC ATGAAGAGTA CTCCAGGCTC TATCAGCTCG CCAAGGAGAC CCCCACCTCT
    CACAGTGATC CTCGTCTTCA GCACGTGCTC ATCTACTTCT TCCAAAATGA AGCGCCTAAA
    AGGGTAGTAG AGCGGACCCT TCTGGAACAA TTTGCCGATA AAAACCTTAG CTATGATGAA
    AGATCGATCA GTATTATGAA GGTGGCTCAA GCAAAACTGA AGGAGATTGG TCCAGATGAC
    ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTACA GTTTGTTTCG AAAGGTGTCT
    GTGTATCTGC TGACAGGCCT GGAACTCTAT CAGAAAGGAA AGTACCAAGA AGCGCTTTCC
    TACCTGGTGT ATGCCTACCA AAGTAATGCC ACTCTGCTGA TGAAGGGGCC CCAGCGGGGC
    GTAAAGGAGT CGGTGATCGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA
    GCAGCTTCTC TCTTTGAAAC AAATGATGAG CACTCTGTAA CAGAGGGTAT TAATGTGATG
    AATGAGCTGA TCATTCCCTG TATTCACCTT ATCATTAATA ATGACATCTC CAAGGATGAC
    CTGGATGCCA TTGAGGTCAT GAGAAACCAC TGGTGCTCTT ACCTTGGGCA GGATATCGCA
    GAAAATCTGC AGCTGTGCTT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA
    ATCATTGTCT TAAAGGAGCC TCCAACTATT CGGCCCAATT CTCCCTATGA CCTTTGTAGC
    CGATTTGCAG CTGTCATGGA GTCAATTCAA GGAGTGTCAG CTGTGACAGT GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810662.1
    CDS70..3366
    Translation

    Target ORF information:

    RefSeq Version XM_010812360.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812360.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
    TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT
    CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT
    GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT
    GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT
    CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA
    GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC
    TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA
    GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT
    CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA
    AATTGTCCAA GTCACACGCA AGATGTGAGT GAGTTCACAC ACAAGCTCCT GGATTGGCTG
    GAGGACGCCT TCCAGCTAGC TGTTAATGTT AATAGCAATC CCAGGAACAA ATCTGAAAAT
    CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTCACGGAAG GGATTCGTGA AGGAAAGCCC
    TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCCCTTCAGG TAAACGGTTA TCGTAACTTA
    GACGAATGTT TAGAAGGGGC CATGGTGGAG GGTGACATTG AGCTGCTTCC TTCCGATCAT
    TCAGTGAAGT ACGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA
    CTCTCAAGAT TTGAGTTCAA TCAGTCCCTG GGTCAGCCAG AGAAAATTCA CAATAAGCTT
    GAATTTCCTC AGATCATTTA TATGGACAGG TATATGTACA GGAGCAAAGA GCTTGTTCGA
    AGTAAGCGAG ATTGTATTCG AAAACTCAAA GAAGAAATAA AAATTCTGCA GCAAAAACTG
    GAAAGGTATG TGAAGTATGG CTCGGGCCCA GCCCGGTTCC CGCTTCCGGA CATGTTGAAA
    TACGTGATTG AATTTGCTAG TACAAAACCT GCCTCAGAGA GCTCTGCATC TCAAAGTGAT
    GCGTGCATGA CAGAACCACT TTCTTCAGCA CACTGCCTGA CTTCTGATCT GACATCCAAG
    GAAAGTACAA GTGAAGAGAG CACGTCTCAG GAAGCTGAAG GAACCTTTTC TTCCTCTGAA
    GATTCTGGAC GCAAGTCTAG GATGGCAGAG CAGCCACTTA AACCTCCCCG TTCTTCCGTG
    GAAATGCCCG CACACCCAGC TCCTCGAACA GTCACAGATG AGGAGATAAA CTTTGTTAAG
    ACCTGCCTGC AGAGGTGGAG GAACGAGATT GAACAGGATA TACAAGACTT AAAGAACAGT
    ATTGCAAGCA CTACCCAGAC TATTGAGCAG ATGTACTGTG ATCCTCTCCT CCGTCAGGTG
    CCTTATCGCT TGCACGCGGT CCTCGTTCAC GAAGGACAGG CAAACGCGGG GCACTACTGG
    GCCTACGTCT ATAATCAGCC GCGACAAGTC TGGCTCAAGT ACAACGACAT CTCTGTTACT
    GAGTCTTCCT GGGAAGAACT TGAAAGAGAT TCCTATGGGG GCCAGAGAAA TGTTAGTGCT
    TACTGCCTGA TGTATATTAA CGACAAGCTG CCCCACTTCA ATGCAGAGGC GGCCCCAAAT
    GAATTAGATC AGATGTCAGG AGAAGTAGAA GCCCTGTCTG TTGAACTTAA GCATTACATT
    CAGGAAGATA ACTGGAGGTT TGAGCAGGAA GTGGAGGAAT GGGAAGAAGA GCAGTCTTGT
    AAAATTCCTC AGATGGCATC TTCCACCAGC TCAGCATCAC AGGACTTCTC TCCATCTCAA
    GAATCTTCAG TAGCCTCTTC CCACGGGGCT CGGTGCCTGT CCTCTGAGCA CGCTGTGATC
    GTGAAGGAGC AGACTGCCCA GGCGATTGCA AACACAGCAC ATGCCTATGA GAACAGTGGC
    GTAGAAGCTG CACTGAGTGA GCTTAAGGAA GCTGAACCCA AGAAGCCCAT GCCCCCGGAA
    ACAAACCTTG CAGAGCAGTC AGAGCAGCCC CCACAGGCTC GTGACGCAGA GTCTGCTGCC
    CAGCCTAATG CTGAGGTCTC TGAAGTCGAG ATTCCCAGTG TGGGAAGGAT TCTGGTTAGA
    TCTGATGCAG ATGGATATGA TGAGGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC
    CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTTATT
    AAGGCATTCC ATGAAGAGTA CTCCAGGCTC TATCAGCTCG CCAAGGAGAC CCCCACCTCT
    CACAGTGATC CTCGTCTTCA GCACGTGCTC ATCTACTTCT TCCAAAATGA AGCGCCTAAA
    AGGGTAGTAG AGCGGACCCT TCTGGAACAA TTTGCCGATA AAAACCTTAG CTATGATGAA
    AGATCGATCA GTATTATGAA GGTGGCTCAA GCAAAACTGA AGGAGATTGG TCCAGATGAC
    ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTACA GTTTGTTTCG AAAGGTGTCT
    GTGTATCTGC TGACAGGCCT GGAACTCTAT CAGAAAGGAA AGTACCAAGA AGCGCTTTCC
    TACCTGGTGT ATGCCTACCA AAGTAATGCC ACTCTGCTGA TGAAGGGGCC CCAGCGGGGC
    GTAAAGGAGT CGGTGATCGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA
    GCAGCTTCTC TCTTTGAAAC AAATGATGAG CACTCTGTAA CAGAGGGTAT TAATGTGATG
    AATGAGCTGA TCATTCCCTG TATTCACCTT ATCATTAATA ATGACATCTC CAAGGATGAC
    CTGGATGCCA TTGAGGTCAT GAGAAACCAC TGGTGCTCTT ACCTTGGGCA GGATATCGCA
    GAAAATCTGC AGCTGTGCTT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA
    ATCATTGTCT TAAAGGAGCC TCCAACTATT CGGCCCAATT CTCCCTATGA CCTTTGTAGC
    CGATTTGCAG CTGTCATGGA GTCAATTCAA GGAGTGTCAG CTGTGACAGT GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.