USP28 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015474610.1	XP_015330096.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
XM_005215811.4	XP_005215868.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
XM_005215812.4	XP_005215869.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
XM_015474609.2	XP_015330095.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
XM_024975129.1	XP_024830897.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X10
XM_005215811.3	XP_005215868.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
XM_005215812.3	XP_005215869.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
XM_010812360.2	XP_010810662.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
XM_005215816.3	XP_005215873.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X7
NM_001192998.1	NP_001179927.1	ubiquitin carboxyl-terminal hydrolase 28
XM_005215817.3	XP_005215874.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X8
XM_005215814.4	XP_005215871.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
XM_005215817.4	XP_005215874.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X8
XM_005215814.3	XP_005215871.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
XM_024975127.1	XP_024830895.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
XM_015474610.2	XP_015330096.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
XM_024975128.1	XP_024830896.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X9
XM_015474609.1	XP_015330095.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
XM_005215816.4	XP_005215873.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222702	XM_024975129.1 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa222700	XM_024975127.1 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa222701	XM_024975128.1 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa92628	XM_015474609.2 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa92629	XM_015474610.2 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa40337	NM_001192998.1 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa26216	XM_005215811.4 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa26217	XM_005215812.4 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa26219	XM_005215814.4 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa26221	XM_005215816.4 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa26222	XM_005215817.4 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa47296	XM_010812360.2 Latest version!	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa26216	XM_005215811.3	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa26217	XM_005215812.3	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa26222	XM_005215817.3	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa26219	XM_005215814.3	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa92629	XM_015474610.1	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa92628	XM_015474609.1	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa26221	XM_005215816.3	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

USP28 ( XM_024975129.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830897.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa222702
Clone ID Related Accession (Same CDS sequence)	XM_024975129.1
Accession Version	XM_024975129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTC 
AATCAGTCCC TGGGTCAGCC AGAGAAAATT CACAATAAGC TTGAATTTCC TCAGATCATT 
TATATGGACA GGTATATGTA CAGGAGCAAA GAGCTTGTTC GAAGTAAGCG AGATTGTATT 
CGAAAACTCA AAGAAGAAAT AAAAATTCTG CAGCAAAAAC TGGAAAGGTA TGTGAAGTAT 
GGCTCGGGCC CAGCCCGGTT CCCGCTTCCG GACATGTTGA AATACGTGAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA GAGCTCTGCA TCTCAAAGTG ATGCGTGCAT GACAGAACCA 
CTTTCTTCAG CACACTGCCT GACTTCTGAT CTGACATCCA AGGAAAGTAC AAGTGAAGAG 
AGCACGTCTC AGGAAGCTGA AGGAACCTTT TCTTCCTCTG AAGATTCTGG ACGCAAGTCT 
AGGATGGCAG AGCAGCCACT TAAACCTCCC CGTTCTTCCG TGGAAATGCC CGCACACCCA 
GCTCCTCGAA CAGTCACAGA TGAGGAGATA AACTTTGTTA AGACCTGCCT GCAGAGGTGG 
AGGAACGAGA TTGAACAGGA TATACAAGAC TTAAAGAACA GTATTGCAAG CACTACCCAG 
ACTATTGAGC AGATGTACTG TGATCCTCTC CTCCGTCAGG TGCCTTATCG CTTGCACGCG 
GTCCTCGTTC ACGAAGGACA GGCAAACGCG GGGCACTACT GGGCCTACGT CTATAATCAG 
CCGCGACAAG TCTGGCTCAA GTACAACGAC ATCTCTGTTA CTGAGTCTTC CTGGGAAGAA 
CTTGAAAGAG ATTCCTATGG GGGCCAGAGA AATGTTAGTG CTTACTGCCT GATGTATATT 
AACGACAAGC TGCCCCACTT CAATGCAGAG GCGGCCCCAA ATGAATTAGA TCAGATGTCA 
GGAGAAGTAG AAGCCCTGTC TGTTGAACTT AAGCATTACA TTCAGGAAGA TAACTGGAGG 
TTTGAGCAGG AAGTGGAGGA ATGGGAAGAA GAGCAGTCTT GTAAAATTCC TCAGATGGCA 
TCTTCCACCA GCTCAGCATC ACAGGACTTC TCTCCATCTC AAGAATCTTC AGTAGCCTCT 
TCCCACGGGG CTCGGTGCCT GTCCTCTGAG CACGCTGTGA TCGTGAAGGA GCAGACTGCC 
CAGGCGATTG CAAACACAGC ACATGCCTAT GAGAACAGTG GCGTAGAAGC TGCACTGAGT 
GAGCTTAAGG AAGCTGAACC CAAGAAGCCC ATGCCCCCGG AAACAAACCT TGCAGAGCAG 
TCAGAGCAGC CCCCACAGGC TCGTGACGCA GAGTCTGCTG CCCAGCCTAA TGCTGAGGTC 
TCTGAAGTCG AGATTCCCAG TGTGGGAAGG ATTCTGGTTA GATCTGATGC AGATGGATAT 
GATGAGGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTTA TTAAGGCATT CCATGAAGAG 
TACTCCAGGC TCTATCAGCT CGCCAAGGAG ACCCCCACCT CTCACAGTGA TCCTCGTCTT 
CAGCACGTGC TCATCTACTT CTTCCAAAAT GAAGCGCCTA AAAGGGTAGT AGAGCGGACC 
CTTCTGGAAC AATTTGCCGA TAAAAACCTT AGCTATGATG AAAGATCGAT CAGTATTATG 
AAGGTGGCTC AAGCAAAACT GAAGGAGATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA CAGTTTGTTT CGAAAGGTGT CTGTGTATCT GCTGACAGGC 
CTGGAACTCT ATCAGAAAGG AAAGTACCAA GAAGCGCTTT CCTACCTGGT GTATGCCTAC 
CAAAGTAATG CCACTCTGCT GATGAAGGGG CCCCAGCGGG GCGTAAAGGA GTCGGTGATC 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTCTTTGAA 
ACAAATGATG AGCACTCTGT AACAGAGGGT ATTAATGTGA TGAATGAGCT GATCATTCCC 
TGTATTCACC TTATCATTAA TAATGACATC TCCAAGGATG ACCTGGATGC CATTGAGGTC 
ATGAGAAACC ACTGGTGCTC TTACCTTGGG CAGGATATCG CAGAAAATCT GCAGCTGTGC 
TTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATTGT CTTAAAGGAG 
CCTCCAACTA TTCGGCCCAA TTCTCCCTAT GACCTTTGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AAGGAGTGTC AGCTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830897.1
CDS	1167..3545
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_024975129.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975129.1

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTC 
AATCAGTCCC TGGGTCAGCC AGAGAAAATT CACAATAAGC TTGAATTTCC TCAGATCATT 
TATATGGACA GGTATATGTA CAGGAGCAAA GAGCTTGTTC GAAGTAAGCG AGATTGTATT 
CGAAAACTCA AAGAAGAAAT AAAAATTCTG CAGCAAAAAC TGGAAAGGTA TGTGAAGTAT 
GGCTCGGGCC CAGCCCGGTT CCCGCTTCCG GACATGTTGA AATACGTGAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA GAGCTCTGCA TCTCAAAGTG ATGCGTGCAT GACAGAACCA 
CTTTCTTCAG CACACTGCCT GACTTCTGAT CTGACATCCA AGGAAAGTAC AAGTGAAGAG 
AGCACGTCTC AGGAAGCTGA AGGAACCTTT TCTTCCTCTG AAGATTCTGG ACGCAAGTCT 
AGGATGGCAG AGCAGCCACT TAAACCTCCC CGTTCTTCCG TGGAAATGCC CGCACACCCA 
GCTCCTCGAA CAGTCACAGA TGAGGAGATA AACTTTGTTA AGACCTGCCT GCAGAGGTGG 
AGGAACGAGA TTGAACAGGA TATACAAGAC TTAAAGAACA GTATTGCAAG CACTACCCAG 
ACTATTGAGC AGATGTACTG TGATCCTCTC CTCCGTCAGG TGCCTTATCG CTTGCACGCG 
GTCCTCGTTC ACGAAGGACA GGCAAACGCG GGGCACTACT GGGCCTACGT CTATAATCAG 
CCGCGACAAG TCTGGCTCAA GTACAACGAC ATCTCTGTTA CTGAGTCTTC CTGGGAAGAA 
CTTGAAAGAG ATTCCTATGG GGGCCAGAGA AATGTTAGTG CTTACTGCCT GATGTATATT 
AACGACAAGC TGCCCCACTT CAATGCAGAG GCGGCCCCAA ATGAATTAGA TCAGATGTCA 
GGAGAAGTAG AAGCCCTGTC TGTTGAACTT AAGCATTACA TTCAGGAAGA TAACTGGAGG 
TTTGAGCAGG AAGTGGAGGA ATGGGAAGAA GAGCAGTCTT GTAAAATTCC TCAGATGGCA 
TCTTCCACCA GCTCAGCATC ACAGGACTTC TCTCCATCTC AAGAATCTTC AGTAGCCTCT 
TCCCACGGGG CTCGGTGCCT GTCCTCTGAG CACGCTGTGA TCGTGAAGGA GCAGACTGCC 
CAGGCGATTG CAAACACAGC ACATGCCTAT GAGAACAGTG GCGTAGAAGC TGCACTGAGT 
GAGCTTAAGG AAGCTGAACC CAAGAAGCCC ATGCCCCCGG AAACAAACCT TGCAGAGCAG 
TCAGAGCAGC CCCCACAGGC TCGTGACGCA GAGTCTGCTG CCCAGCCTAA TGCTGAGGTC 
TCTGAAGTCG AGATTCCCAG TGTGGGAAGG ATTCTGGTTA GATCTGATGC AGATGGATAT 
GATGAGGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTTA TTAAGGCATT CCATGAAGAG 
TACTCCAGGC TCTATCAGCT CGCCAAGGAG ACCCCCACCT CTCACAGTGA TCCTCGTCTT 
CAGCACGTGC TCATCTACTT CTTCCAAAAT GAAGCGCCTA AAAGGGTAGT AGAGCGGACC 
CTTCTGGAAC AATTTGCCGA TAAAAACCTT AGCTATGATG AAAGATCGAT CAGTATTATG 
AAGGTGGCTC AAGCAAAACT GAAGGAGATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA CAGTTTGTTT CGAAAGGTGT CTGTGTATCT GCTGACAGGC 
CTGGAACTCT ATCAGAAAGG AAAGTACCAA GAAGCGCTTT CCTACCTGGT GTATGCCTAC 
CAAAGTAATG CCACTCTGCT GATGAAGGGG CCCCAGCGGG GCGTAAAGGA GTCGGTGATC 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTCTTTGAA 
ACAAATGATG AGCACTCTGT AACAGAGGGT ATTAATGTGA TGAATGAGCT GATCATTCCC 
TGTATTCACC TTATCATTAA TAATGACATC TCCAAGGATG ACCTGGATGC CATTGAGGTC 
ATGAGAAACC ACTGGTGCTC TTACCTTGGG CAGGATATCG CAGAAAATCT GCAGCTGTGC 
TTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATTGT CTTAAAGGAG 
CCTCCAACTA TTCGGCCCAA TTCTCCCTAT GACCTTTGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AAGGAGTGTC AGCTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_024975127.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830895.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa222700
Clone ID Related Accession (Same CDS sequence)	XM_024975127.1
Accession Version	XM_024975127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG 
CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC 
GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC 
ATCCTGGCCA TAGCTAAAGC CCGTCAGACC TTTGACCGAG ATGGGTCTGA AGCAGGGCTT 
ATTAAGGCAT TCCATGAAGA GTACTCCAGG CTCTATCAGC TCGCCAAGGA GACCCCCACC 
TCTCACAGTG ATCCTCGTCT TCAGCACGTG CTCATCTACT TCTTCCAAAA TGAAGCGCCT 
AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA CAATTTGCCG ATAAAAACCT TAGCTATGAT 
GAAAGATCGA TCAGTATTAT GAAGGTGGCT CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ACAGTTTGTT TCGAAAGGTG 
TCTGTGTATC TGCTGACAGG CCTGGAACTC TATCAGAAAG GAAAGTACCA AGAAGCGCTT 
TCCTACCTGG TGTATGCCTA CCAAAGTAAT GCCACTCTGC TGATGAAGGG GCCCCAGCGG 
GGCGTAAAGG AGTCGGTGAT CGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTCTTTGA AACAAATGAT GAGCACTCTG TAACAGAGGG TATTAATGTG 
ATGAATGAGC TGATCATTCC CTGTATTCAC CTTATCATTA ATAATGACAT CTCCAAGGAT 
GACCTGGATG CCATTGAGGT CATGAGAAAC CACTGGTGCT CTTACCTTGG GCAGGATATC 
GCAGAAAATC TGCAGCTGTG CTTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATTG TCTTAAAGGA GCCTCCAACT ATTCGGCCCA ATTCTCCCTA TGACCTTTGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830895.1
CDS	87..3446
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_024975127.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975127.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG 
CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC 
GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC 
ATCCTGGCCA TAGCTAAAGC CCGTCAGACC TTTGACCGAG ATGGGTCTGA AGCAGGGCTT 
ATTAAGGCAT TCCATGAAGA GTACTCCAGG CTCTATCAGC TCGCCAAGGA GACCCCCACC 
TCTCACAGTG ATCCTCGTCT TCAGCACGTG CTCATCTACT TCTTCCAAAA TGAAGCGCCT 
AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA CAATTTGCCG ATAAAAACCT TAGCTATGAT 
GAAAGATCGA TCAGTATTAT GAAGGTGGCT CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ACAGTTTGTT TCGAAAGGTG 
TCTGTGTATC TGCTGACAGG CCTGGAACTC TATCAGAAAG GAAAGTACCA AGAAGCGCTT 
TCCTACCTGG TGTATGCCTA CCAAAGTAAT GCCACTCTGC TGATGAAGGG GCCCCAGCGG 
GGCGTAAAGG AGTCGGTGAT CGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTCTTTGA AACAAATGAT GAGCACTCTG TAACAGAGGG TATTAATGTG 
ATGAATGAGC TGATCATTCC CTGTATTCAC CTTATCATTA ATAATGACAT CTCCAAGGAT 
GACCTGGATG CCATTGAGGT CATGAGAAAC CACTGGTGCT CTTACCTTGG GCAGGATATC 
GCAGAAAATC TGCAGCTGTG CTTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATTG TCTTAAAGGA GCCTCCAACT ATTCGGCCCA ATTCTCCCTA TGACCTTTGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_024975128.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830896.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa222701
Clone ID Related Accession (Same CDS sequence)	XM_024975128.1
Accession Version	XM_024975128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG 
CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC 
GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG 
GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA 
AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC 
CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG 
ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG 
TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC 
CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG 
GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG 
GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG 
GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC 
ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT 
GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA 
GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC 
TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG 
ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830896.1
CDS	87..3218
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_024975128.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975128.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG 
CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC 
GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG 
GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA 
AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC 
CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG 
ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG 
TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC 
CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG 
GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG 
GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG 
GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC 
ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT 
GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA 
GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC 
TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG 
ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015474609.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330095.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa92628
Clone ID Related Accession (Same CDS sequence)	XM_015474609.2 , XM_015474609.1
Accession Version	XM_015474609.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474609.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG 
GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC 
AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC 
CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA 
AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG 
GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT 
TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG 
TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG 
AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA 
GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT 
GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC 
CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT 
CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330095.1
CDS	87..3341
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_015474609.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474609.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG 
GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC 
AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC 
CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA 
AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG 
GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT 
TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG 
TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG 
AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA 
GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT 
GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC 
CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT 
CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015474609.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330095.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

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CloneID	OBa92628
Clone ID Related Accession (Same CDS sequence)	XM_015474609.2 , XM_015474609.1
Accession Version	XM_015474609.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG 
GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC 
AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC 
CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA 
AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG 
GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT 
TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG 
TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG 
AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA 
GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT 
GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC 
CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT 
CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330095.1
CDS	70..3324
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_015474609.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474609.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG 
GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC 
AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC 
CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA 
AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG 
GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT 
TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG 
TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG 
AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA 
GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT 
GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC 
CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT 
CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015474610.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330096.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

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CloneID	OBa92629
Clone ID Related Accession (Same CDS sequence)	XM_015474610.1 , XM_015474610.2
Accession Version	XM_015474610.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG TGATGCTGAG CCCTGCCATG 
CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC CGTCAGACCT TTGACCGAGA TGGGTCTGAA 
GCAGGGCTTA TTAAGGCATT CCATGAAGAG TACTCCAGGC TCTATCAGCT CGCCAAGGAG 
ACCCCCACCT CTCACAGTGA TCCTCGTCTT CAGCACGTGC TCATCTACTT CTTCCAAAAT 
GAAGCGCCTA AAAGGGTAGT AGAGCGGACC CTTCTGGAAC AATTTGCCGA TAAAAACCTT 
AGCTATGATG AAAGATCGAT CAGTATTATG AAGGTGGCTC AAGCAAAACT GAAGGAGATT 
GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA CAGTTTGTTT 
CGAAAGGTGT CTGTGTATCT GCTGACAGGC CTGGAACTCT ATCAGAAAGG AAAGTACCAA 
GAAGCGCTTT CCTACCTGGT GTATGCCTAC CAAAGTAATG CCACTCTGCT GATGAAGGGG 
CCCCAGCGGG GCGTAAAGGA GTCGGTGATC GCTTTATACC GAAGAAAATG CCTTCTGGAG 
CTGAATGCCA AAGCAGCTTC TCTCTTTGAA ACAAATGATG AGCACTCTGT AACAGAGGGT 
ATTAATGTGA TGAATGAGCT GATCATTCCC TGTATTCACC TTATCATTAA TAATGACATC 
TCCAAGGATG ACCTGGATGC CATTGAGGTC ATGAGAAACC ACTGGTGCTC TTACCTTGGG 
CAGGATATCG CAGAAAATCT GCAGCTGTGC TTAGGGGAGT TTCTACCCAG ACTTCTAGAT 
CCTTCTGCAG AAATCATTGT CTTAAAGGAG CCTCCAACTA TTCGGCCCAA TTCTCCCTAT 
GACCTTTGTA GCCGATTTGC AGCTGTCATG GAGTCAATTC AAGGAGTGTC AGCTGTGACA 
GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330096.1
CDS	70..3318
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_015474610.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474610.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG TGATGCTGAG CCCTGCCATG 
CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC CGTCAGACCT TTGACCGAGA TGGGTCTGAA 
GCAGGGCTTA TTAAGGCATT CCATGAAGAG TACTCCAGGC TCTATCAGCT CGCCAAGGAG 
ACCCCCACCT CTCACAGTGA TCCTCGTCTT CAGCACGTGC TCATCTACTT CTTCCAAAAT 
GAAGCGCCTA AAAGGGTAGT AGAGCGGACC CTTCTGGAAC AATTTGCCGA TAAAAACCTT 
AGCTATGATG AAAGATCGAT CAGTATTATG AAGGTGGCTC AAGCAAAACT GAAGGAGATT 
GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA CAGTTTGTTT 
CGAAAGGTGT CTGTGTATCT GCTGACAGGC CTGGAACTCT ATCAGAAAGG AAAGTACCAA 
GAAGCGCTTT CCTACCTGGT GTATGCCTAC CAAAGTAATG CCACTCTGCT GATGAAGGGG 
CCCCAGCGGG GCGTAAAGGA GTCGGTGATC GCTTTATACC GAAGAAAATG CCTTCTGGAG 
CTGAATGCCA AAGCAGCTTC TCTCTTTGAA ACAAATGATG AGCACTCTGT AACAGAGGGT 
ATTAATGTGA TGAATGAGCT GATCATTCCC TGTATTCACC TTATCATTAA TAATGACATC 
TCCAAGGATG ACCTGGATGC CATTGAGGTC ATGAGAAACC ACTGGTGCTC TTACCTTGGG 
CAGGATATCG CAGAAAATCT GCAGCTGTGC TTAGGGGAGT TTCTACCCAG ACTTCTAGAT 
CCTTCTGCAG AAATCATTGT CTTAAAGGAG CCTCCAACTA TTCGGCCCAA TTCTCCCTAT 
GACCTTTGTA GCCGATTTGC AGCTGTCATG GAGTCAATTC AAGGAGTGTC AGCTGTGACA 
GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015474610.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330096.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

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CloneID	OBa92629
Clone ID Related Accession (Same CDS sequence)	XM_015474610.1 , XM_015474610.2
Accession Version	XM_015474610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG TGATGCTGAG CCCTGCCATG 
CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC CGTCAGACCT TTGACCGAGA TGGGTCTGAA 
GCAGGGCTTA TTAAGGCATT CCATGAAGAG TACTCCAGGC TCTATCAGCT CGCCAAGGAG 
ACCCCCACCT CTCACAGTGA TCCTCGTCTT CAGCACGTGC TCATCTACTT CTTCCAAAAT 
GAAGCGCCTA AAAGGGTAGT AGAGCGGACC CTTCTGGAAC AATTTGCCGA TAAAAACCTT 
AGCTATGATG AAAGATCGAT CAGTATTATG AAGGTGGCTC AAGCAAAACT GAAGGAGATT 
GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA CAGTTTGTTT 
CGAAAGGTGT CTGTGTATCT GCTGACAGGC CTGGAACTCT ATCAGAAAGG AAAGTACCAA 
GAAGCGCTTT CCTACCTGGT GTATGCCTAC CAAAGTAATG CCACTCTGCT GATGAAGGGG 
CCCCAGCGGG GCGTAAAGGA GTCGGTGATC GCTTTATACC GAAGAAAATG CCTTCTGGAG 
CTGAATGCCA AAGCAGCTTC TCTCTTTGAA ACAAATGATG AGCACTCTGT AACAGAGGGT 
ATTAATGTGA TGAATGAGCT GATCATTCCC TGTATTCACC TTATCATTAA TAATGACATC 
TCCAAGGATG ACCTGGATGC CATTGAGGTC ATGAGAAACC ACTGGTGCTC TTACCTTGGG 
CAGGATATCG CAGAAAATCT GCAGCTGTGC TTAGGGGAGT TTCTACCCAG ACTTCTAGAT 
CCTTCTGCAG AAATCATTGT CTTAAAGGAG CCTCCAACTA TTCGGCCCAA TTCTCCCTAT 
GACCTTTGTA GCCGATTTGC AGCTGTCATG GAGTCAATTC AAGGAGTGTC AGCTGTGACA 
GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330096.1
CDS	87..3335
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_015474610.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474610.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG TGATGCTGAG CCCTGCCATG 
CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC CGTCAGACCT TTGACCGAGA TGGGTCTGAA 
GCAGGGCTTA TTAAGGCATT CCATGAAGAG TACTCCAGGC TCTATCAGCT CGCCAAGGAG 
ACCCCCACCT CTCACAGTGA TCCTCGTCTT CAGCACGTGC TCATCTACTT CTTCCAAAAT 
GAAGCGCCTA AAAGGGTAGT AGAGCGGACC CTTCTGGAAC AATTTGCCGA TAAAAACCTT 
AGCTATGATG AAAGATCGAT CAGTATTATG AAGGTGGCTC AAGCAAAACT GAAGGAGATT 
GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA CAGTTTGTTT 
CGAAAGGTGT CTGTGTATCT GCTGACAGGC CTGGAACTCT ATCAGAAAGG AAAGTACCAA 
GAAGCGCTTT CCTACCTGGT GTATGCCTAC CAAAGTAATG CCACTCTGCT GATGAAGGGG 
CCCCAGCGGG GCGTAAAGGA GTCGGTGATC GCTTTATACC GAAGAAAATG CCTTCTGGAG 
CTGAATGCCA AAGCAGCTTC TCTCTTTGAA ACAAATGATG AGCACTCTGT AACAGAGGGT 
ATTAATGTGA TGAATGAGCT GATCATTCCC TGTATTCACC TTATCATTAA TAATGACATC 
TCCAAGGATG ACCTGGATGC CATTGAGGTC ATGAGAAACC ACTGGTGCTC TTACCTTGGG 
CAGGATATCG CAGAAAATCT GCAGCTGTGC TTAGGGGAGT TTCTACCCAG ACTTCTAGAT 
CCTTCTGCAG AAATCATTGT CTTAAAGGAG CCTCCAACTA TTCGGCCCAA TTCTCCCTAT 
GACCTTTGTA GCCGATTTGC AGCTGTCATG GAGTCAATTC AAGGAGTGTC AGCTGTGACA 
GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001192998.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179927.1 Bos taurus ubiquitin specific peptidase 28 (USP28), mRNA.

Price & Availability↑

CloneID	OBa40337
Clone ID Related Accession (Same CDS sequence)	NM_001192998.1
Accession Version	NM_001192998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On Jul 17, 2010 this sequence version replaced XM_865093.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGTGA TGCTGAGCCC TGCCATGCAA 
GGGGTCATCC TGGCCATAGC TAAAGCCCGT CAGACCTTTG ACCGAGATGG GTCTGAAGCA 
GGGCTTATTA AGGCATTCCA TGAAGAGTAC TCCAGGCTCT ATCAGCTCGC CAAGGAGACC 
CCCACCTCTC ACAGTGATCC TCGTCTTCAG CACGTGCTCA TCTACTTCTT CCAAAATGAA 
GCGCCTAAAA GGGTAGTAGA GCGGACCCTT CTGGAACAAT TTGCCGATAA AAACCTTAGC 
TATGATGAAA GATCGATCAG TATTATGAAG GTGGCTCAAG CAAAACTGAA GGAGATTGGT 
CCAGATGACA TGAATATGGA AGAGTACAAG AAGTGGCATG AAGATTACAG TTTGTTTCGA 
AAGGTGTCTG TGTATCTGCT GACAGGCCTG GAACTCTATC AGAAAGGAAA GTACCAAGAA 
GCGCTTTCCT ACCTGGTGTA TGCCTACCAA AGTAATGCCA CTCTGCTGAT GAAGGGGCCC 
CAGCGGGGCG TAAAGGAGTC GGTGATCGCT TTATACCGAA GAAAATGCCT TCTGGAGCTG 
AATGCCAAAG CAGCTTCTCT CTTTGAAACA AATGATGAGC ACTCTGTAAC AGAGGGTATT 
AATGTGATGA ATGAGCTGAT CATTCCCTGT ATTCACCTTA TCATTAATAA TGACATCTCC 
AAGGATGACC TGGATGCCAT TGAGGTCATG AGAAACCACT GGTGCTCTTA CCTTGGGCAG 
GATATCGCAG AAAATCTGCA GCTGTGCTTA GGGGAGTTTC TACCCAGACT TCTAGATCCT 
TCTGCAGAAA TCATTGTCTT AAAGGAGCCT CCAACTATTC GGCCCAATTC TCCCTATGAC 
CTTTGTAGCC GATTTGCAGC TGTCATGGAG TCAATTCAAG GAGTGTCAGC TGTGACAGTG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179927.1
CDS	32..3277
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	NM_001192998.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192998.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGTGA TGCTGAGCCC TGCCATGCAA 
GGGGTCATCC TGGCCATAGC TAAAGCCCGT CAGACCTTTG ACCGAGATGG GTCTGAAGCA 
GGGCTTATTA AGGCATTCCA TGAAGAGTAC TCCAGGCTCT ATCAGCTCGC CAAGGAGACC 
CCCACCTCTC ACAGTGATCC TCGTCTTCAG CACGTGCTCA TCTACTTCTT CCAAAATGAA 
GCGCCTAAAA GGGTAGTAGA GCGGACCCTT CTGGAACAAT TTGCCGATAA AAACCTTAGC 
TATGATGAAA GATCGATCAG TATTATGAAG GTGGCTCAAG CAAAACTGAA GGAGATTGGT 
CCAGATGACA TGAATATGGA AGAGTACAAG AAGTGGCATG AAGATTACAG TTTGTTTCGA 
AAGGTGTCTG TGTATCTGCT GACAGGCCTG GAACTCTATC AGAAAGGAAA GTACCAAGAA 
GCGCTTTCCT ACCTGGTGTA TGCCTACCAA AGTAATGCCA CTCTGCTGAT GAAGGGGCCC 
CAGCGGGGCG TAAAGGAGTC GGTGATCGCT TTATACCGAA GAAAATGCCT TCTGGAGCTG 
AATGCCAAAG CAGCTTCTCT CTTTGAAACA AATGATGAGC ACTCTGTAAC AGAGGGTATT 
AATGTGATGA ATGAGCTGAT CATTCCCTGT ATTCACCTTA TCATTAATAA TGACATCTCC 
AAGGATGACC TGGATGCCAT TGAGGTCATG AGAAACCACT GGTGCTCTTA CCTTGGGCAG 
GATATCGCAG AAAATCTGCA GCTGTGCTTA GGGGAGTTTC TACCCAGACT TCTAGATCCT 
TCTGCAGAAA TCATTGTCTT AAAGGAGCCT CCAACTATTC GGCCCAATTC TCCCTATGAC 
CTTTGTAGCC GATTTGCAGC TGTCATGGAG TCAATTCAAG GAGTGTCAGC TGTGACAGTG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215811.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215868.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26216
Clone ID Related Accession (Same CDS sequence)	XM_005215811.4 , XM_005215811.3
Accession Version	XM_005215811.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215811.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG 
GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC 
AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC 
CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA 
AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG 
GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT 
TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG 
TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG 
AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA 
GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT 
GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC 
CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT 
CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215868.1
CDS	87..3521
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215811.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215811.4

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG 
GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC 
AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC 
CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA 
AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG 
GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT 
TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG 
TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG 
AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA 
GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT 
GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC 
CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT 
CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215811.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215868.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

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CloneID	OBa26216
Clone ID Related Accession (Same CDS sequence)	XM_005215811.4 , XM_005215811.3
Accession Version	XM_005215811.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG 
GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC 
AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC 
CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA 
AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG 
GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT 
TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG 
TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG 
AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA 
GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT 
GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC 
CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT 
CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215868.1
CDS	70..3504
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215811.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215811.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG 
GGAAGGATTC TGGTTAGATC TGATGCAGAT GGATATGATG AGGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTTATTAA GGCATTCCAT GAAGAGTACT CCAGGCTCTA TCAGCTCGCC 
AAGGAGACCC CCACCTCTCA CAGTGATCCT CGTCTTCAGC ACGTGCTCAT CTACTTCTTC 
CAAAATGAAG CGCCTAAAAG GGTAGTAGAG CGGACCCTTC TGGAACAATT TGCCGATAAA 
AACCTTAGCT ATGATGAAAG ATCGATCAGT ATTATGAAGG TGGCTCAAGC AAAACTGAAG 
GAGATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTACAGT 
TTGTTTCGAA AGGTGTCTGT GTATCTGCTG ACAGGCCTGG AACTCTATCA GAAAGGAAAG 
TACCAAGAAG CGCTTTCCTA CCTGGTGTAT GCCTACCAAA GTAATGCCAC TCTGCTGATG 
AAGGGGCCCC AGCGGGGCGT AAAGGAGTCG GTGATCGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTC TTTGAAACAA ATGATGAGCA CTCTGTAACA 
GAGGGTATTA ATGTGATGAA TGAGCTGATC ATTCCCTGTA TTCACCTTAT CATTAATAAT 
GACATCTCCA AGGATGACCT GGATGCCATT GAGGTCATGA GAAACCACTG GTGCTCTTAC 
CTTGGGCAGG ATATCGCAGA AAATCTGCAG CTGTGCTTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATTGTCTTA AAGGAGCCTC CAACTATTCG GCCCAATTCT 
CCCTATGACC TTTGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAAGG AGTGTCAGCT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215812.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215869.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa26217
Clone ID Related Accession (Same CDS sequence)	XM_005215812.4 , XM_005215812.3
Accession Version	XM_005215812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215812.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG 
CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC 
GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG 
GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA 
AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC 
CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG 
ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG 
TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC 
CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG 
GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG 
GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG 
GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC 
ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT 
GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA 
GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC 
TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG 
ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215869.1
CDS	87..3518
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215812.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215812.4

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG 
CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC 
GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG 
GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA 
AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC 
CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG 
ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG 
TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC 
CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG 
GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG 
GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG 
GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC 
ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT 
GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA 
GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC 
TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG 
ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215812.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215869.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

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CloneID	OBa26217
Clone ID Related Accession (Same CDS sequence)	XM_005215812.4 , XM_005215812.3
Accession Version	XM_005215812.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG 
CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC 
GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG 
GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA 
AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC 
CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG 
ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG 
TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC 
CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG 
GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG 
GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG 
GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC 
ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT 
GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA 
GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC 
TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG 
ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215869.1
CDS	70..3501
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215812.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215812.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGCTTA AGGAAGCTGA ACCCAAGAAG 
CCCATGCCCC CGGAAACAAA CCTTGCAGAG CAGTCAGAGC AGCCCCCACA GGCTCGTGAC 
GCAGAGTCTG CTGCCCAGCC TAATGCTGAG GTCTCTGAAG TCGAGATTCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGA TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCATCCTGGC CATAGCTAAA GCCCGTCAGA CCTTTGACCG AGATGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTCCATGAA GAGTACTCCA GGCTCTATCA GCTCGCCAAG 
GAGACCCCCA CCTCTCACAG TGATCCTCGT CTTCAGCACG TGCTCATCTA CTTCTTCCAA 
AATGAAGCGC CTAAAAGGGT AGTAGAGCGG ACCCTTCTGG AACAATTTGC CGATAAAAAC 
CTTAGCTATG ATGAAAGATC GATCAGTATT ATGAAGGTGG CTCAAGCAAA ACTGAAGGAG 
ATTGGTCCAG ATGACATGAA TATGGAAGAG TACAAGAAGT GGCATGAAGA TTACAGTTTG 
TTTCGAAAGG TGTCTGTGTA TCTGCTGACA GGCCTGGAAC TCTATCAGAA AGGAAAGTAC 
CAAGAAGCGC TTTCCTACCT GGTGTATGCC TACCAAAGTA ATGCCACTCT GCTGATGAAG 
GGGCCCCAGC GGGGCGTAAA GGAGTCGGTG ATCGCTTTAT ACCGAAGAAA ATGCCTTCTG 
GAGCTGAATG CCAAAGCAGC TTCTCTCTTT GAAACAAATG ATGAGCACTC TGTAACAGAG 
GGTATTAATG TGATGAATGA GCTGATCATT CCCTGTATTC ACCTTATCAT TAATAATGAC 
ATCTCCAAGG ATGACCTGGA TGCCATTGAG GTCATGAGAA ACCACTGGTG CTCTTACCTT 
GGGCAGGATA TCGCAGAAAA TCTGCAGCTG TGCTTAGGGG AGTTTCTACC CAGACTTCTA 
GATCCTTCTG CAGAAATCAT TGTCTTAAAG GAGCCTCCAA CTATTCGGCC CAATTCTCCC 
TATGACCTTT GTAGCCGATT TGCAGCTGTC ATGGAGTCAA TTCAAGGAGT GTCAGCTGTG 
ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215814.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215871.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa26219
Clone ID Related Accession (Same CDS sequence)	XM_005215814.4 , XM_005215814.3
Accession Version	XM_005215814.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215814.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTGATGC TGAGCCCTGC CATGCAAGGG 
GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG 
CTTATTAAGG CATTCCATGA AGAGTACTCC AGGCTCTATC AGCTCGCCAA GGAGACCCCC 
ACCTCTCACA GTGATCCTCG TCTTCAGCAC GTGCTCATCT ACTTCTTCCA AAATGAAGCG 
CCTAAAAGGG TAGTAGAGCG GACCCTTCTG GAACAATTTG CCGATAAAAA CCTTAGCTAT 
GATGAAAGAT CGATCAGTAT TATGAAGGTG GCTCAAGCAA AACTGAAGGA GATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTACAGTTT GTTTCGAAAG 
GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA CTCTATCAGA AAGGAAAGTA CCAAGAAGCG 
CTTTCCTACC TGGTGTATGC CTACCAAAGT AATGCCACTC TGCTGATGAA GGGGCCCCAG 
CGGGGCGTAA AGGAGTCGGT GATCGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTCTT TGAAACAAAT GATGAGCACT CTGTAACAGA GGGTATTAAT 
GTGATGAATG AGCTGATCAT TCCCTGTATT CACCTTATCA TTAATAATGA CATCTCCAAG 
GATGACCTGG ATGCCATTGA GGTCATGAGA AACCACTGGT GCTCTTACCT TGGGCAGGAT 
ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TTGTCTTAAA GGAGCCTCCA ACTATTCGGC CCAATTCTCC CTATGACCTT 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAAGGAG TGTCAGCTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215871.1
CDS	87..3449
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215814.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215814.4

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTGATGC TGAGCCCTGC CATGCAAGGG 
GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG 
CTTATTAAGG CATTCCATGA AGAGTACTCC AGGCTCTATC AGCTCGCCAA GGAGACCCCC 
ACCTCTCACA GTGATCCTCG TCTTCAGCAC GTGCTCATCT ACTTCTTCCA AAATGAAGCG 
CCTAAAAGGG TAGTAGAGCG GACCCTTCTG GAACAATTTG CCGATAAAAA CCTTAGCTAT 
GATGAAAGAT CGATCAGTAT TATGAAGGTG GCTCAAGCAA AACTGAAGGA GATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTACAGTTT GTTTCGAAAG 
GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA CTCTATCAGA AAGGAAAGTA CCAAGAAGCG 
CTTTCCTACC TGGTGTATGC CTACCAAAGT AATGCCACTC TGCTGATGAA GGGGCCCCAG 
CGGGGCGTAA AGGAGTCGGT GATCGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTCTT TGAAACAAAT GATGAGCACT CTGTAACAGA GGGTATTAAT 
GTGATGAATG AGCTGATCAT TCCCTGTATT CACCTTATCA TTAATAATGA CATCTCCAAG 
GATGACCTGG ATGCCATTGA GGTCATGAGA AACCACTGGT GCTCTTACCT TGGGCAGGAT 
ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TTGTCTTAAA GGAGCCTCCA ACTATTCGGC CCAATTCTCC CTATGACCTT 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAAGGAG TGTCAGCTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215814.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215871.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa26219
Clone ID Related Accession (Same CDS sequence)	XM_005215814.4 , XM_005215814.3
Accession Version	XM_005215814.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215814.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTGATGC TGAGCCCTGC CATGCAAGGG 
GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG 
CTTATTAAGG CATTCCATGA AGAGTACTCC AGGCTCTATC AGCTCGCCAA GGAGACCCCC 
ACCTCTCACA GTGATCCTCG TCTTCAGCAC GTGCTCATCT ACTTCTTCCA AAATGAAGCG 
CCTAAAAGGG TAGTAGAGCG GACCCTTCTG GAACAATTTG CCGATAAAAA CCTTAGCTAT 
GATGAAAGAT CGATCAGTAT TATGAAGGTG GCTCAAGCAA AACTGAAGGA GATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTACAGTTT GTTTCGAAAG 
GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA CTCTATCAGA AAGGAAAGTA CCAAGAAGCG 
CTTTCCTACC TGGTGTATGC CTACCAAAGT AATGCCACTC TGCTGATGAA GGGGCCCCAG 
CGGGGCGTAA AGGAGTCGGT GATCGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTCTT TGAAACAAAT GATGAGCACT CTGTAACAGA GGGTATTAAT 
GTGATGAATG AGCTGATCAT TCCCTGTATT CACCTTATCA TTAATAATGA CATCTCCAAG 
GATGACCTGG ATGCCATTGA GGTCATGAGA AACCACTGGT GCTCTTACCT TGGGCAGGAT 
ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TTGTCTTAAA GGAGCCTCCA ACTATTCGGC CCAATTCTCC CTATGACCTT 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAAGGAG TGTCAGCTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215871.1
CDS	70..3432
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215814.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215814.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGC TTAAGGAAGC TGAACCCAAG 
AAGCCCATGC CCCCGGAAAC AAACCTTGCA GAGCAGTCAG AGCAGCCCCC ACAGGCTCGT 
GACGCAGAGT CTGCTGCCCA GCCTAATGCT GAGGTGATGC TGAGCCCTGC CATGCAAGGG 
GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG 
CTTATTAAGG CATTCCATGA AGAGTACTCC AGGCTCTATC AGCTCGCCAA GGAGACCCCC 
ACCTCTCACA GTGATCCTCG TCTTCAGCAC GTGCTCATCT ACTTCTTCCA AAATGAAGCG 
CCTAAAAGGG TAGTAGAGCG GACCCTTCTG GAACAATTTG CCGATAAAAA CCTTAGCTAT 
GATGAAAGAT CGATCAGTAT TATGAAGGTG GCTCAAGCAA AACTGAAGGA GATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTACAGTTT GTTTCGAAAG 
GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA CTCTATCAGA AAGGAAAGTA CCAAGAAGCG 
CTTTCCTACC TGGTGTATGC CTACCAAAGT AATGCCACTC TGCTGATGAA GGGGCCCCAG 
CGGGGCGTAA AGGAGTCGGT GATCGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTCTT TGAAACAAAT GATGAGCACT CTGTAACAGA GGGTATTAAT 
GTGATGAATG AGCTGATCAT TCCCTGTATT CACCTTATCA TTAATAATGA CATCTCCAAG 
GATGACCTGG ATGCCATTGA GGTCATGAGA AACCACTGGT GCTCTTACCT TGGGCAGGAT 
ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TTGTCTTAAA GGAGCCTCCA ACTATTCGGC CCAATTCTCC CTATGACCTT 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAAGGAG TGTCAGCTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215816.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215873.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa26221
Clone ID Related Accession (Same CDS sequence)	XM_005215816.3 , XM_005215816.4
Accession Version	XM_005215816.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG CATTCCATGA AGAGTACTCC 
AGGCTCTATC AGCTCGCCAA GGAGACCCCC ACCTCTCACA GTGATCCTCG TCTTCAGCAC 
GTGCTCATCT ACTTCTTCCA AAATGAAGCG CCTAAAAGGG TAGTAGAGCG GACCCTTCTG 
GAACAATTTG CCGATAAAAA CCTTAGCTAT GATGAAAGAT CGATCAGTAT TATGAAGGTG 
GCTCAAGCAA AACTGAAGGA GATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG 
TGGCATGAAG ATTACAGTTT GTTTCGAAAG GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA 
CTCTATCAGA AAGGAAAGTA CCAAGAAGCG CTTTCCTACC TGGTGTATGC CTACCAAAGT 
AATGCCACTC TGCTGATGAA GGGGCCCCAG CGGGGCGTAA AGGAGTCGGT GATCGCTTTA 
TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTCTT TGAAACAAAT 
GATGAGCACT CTGTAACAGA GGGTATTAAT GTGATGAATG AGCTGATCAT TCCCTGTATT 
CACCTTATCA TTAATAATGA CATCTCCAAG GATGACCTGG ATGCCATTGA GGTCATGAGA 
AACCACTGGT GCTCTTACCT TGGGCAGGAT ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG 
GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TTGTCTTAAA GGAGCCTCCA 
ACTATTCGGC CCAATTCTCC CTATGACCTT TGTAGCCGAT TTGCAGCTGT CATGGAGTCA 
ATTCAAGGAG TGTCAGCTGT GACAGTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215873.1
CDS	70..3222
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215816.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215816.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG CATTCCATGA AGAGTACTCC 
AGGCTCTATC AGCTCGCCAA GGAGACCCCC ACCTCTCACA GTGATCCTCG TCTTCAGCAC 
GTGCTCATCT ACTTCTTCCA AAATGAAGCG CCTAAAAGGG TAGTAGAGCG GACCCTTCTG 
GAACAATTTG CCGATAAAAA CCTTAGCTAT GATGAAAGAT CGATCAGTAT TATGAAGGTG 
GCTCAAGCAA AACTGAAGGA GATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG 
TGGCATGAAG ATTACAGTTT GTTTCGAAAG GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA 
CTCTATCAGA AAGGAAAGTA CCAAGAAGCG CTTTCCTACC TGGTGTATGC CTACCAAAGT 
AATGCCACTC TGCTGATGAA GGGGCCCCAG CGGGGCGTAA AGGAGTCGGT GATCGCTTTA 
TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTCTT TGAAACAAAT 
GATGAGCACT CTGTAACAGA GGGTATTAAT GTGATGAATG AGCTGATCAT TCCCTGTATT 
CACCTTATCA TTAATAATGA CATCTCCAAG GATGACCTGG ATGCCATTGA GGTCATGAGA 
AACCACTGGT GCTCTTACCT TGGGCAGGAT ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG 
GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TTGTCTTAAA GGAGCCTCCA 
ACTATTCGGC CCAATTCTCC CTATGACCTT TGTAGCCGAT TTGCAGCTGT CATGGAGTCA 
ATTCAAGGAG TGTCAGCTGT GACAGTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215816.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215873.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa26221
Clone ID Related Accession (Same CDS sequence)	XM_005215816.3 , XM_005215816.4
Accession Version	XM_005215816.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215816.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG CATTCCATGA AGAGTACTCC 
AGGCTCTATC AGCTCGCCAA GGAGACCCCC ACCTCTCACA GTGATCCTCG TCTTCAGCAC 
GTGCTCATCT ACTTCTTCCA AAATGAAGCG CCTAAAAGGG TAGTAGAGCG GACCCTTCTG 
GAACAATTTG CCGATAAAAA CCTTAGCTAT GATGAAAGAT CGATCAGTAT TATGAAGGTG 
GCTCAAGCAA AACTGAAGGA GATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG 
TGGCATGAAG ATTACAGTTT GTTTCGAAAG GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA 
CTCTATCAGA AAGGAAAGTA CCAAGAAGCG CTTTCCTACC TGGTGTATGC CTACCAAAGT 
AATGCCACTC TGCTGATGAA GGGGCCCCAG CGGGGCGTAA AGGAGTCGGT GATCGCTTTA 
TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTCTT TGAAACAAAT 
GATGAGCACT CTGTAACAGA GGGTATTAAT GTGATGAATG AGCTGATCAT TCCCTGTATT 
CACCTTATCA TTAATAATGA CATCTCCAAG GATGACCTGG ATGCCATTGA GGTCATGAGA 
AACCACTGGT GCTCTTACCT TGGGCAGGAT ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG 
GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TTGTCTTAAA GGAGCCTCCA 
ACTATTCGGC CCAATTCTCC CTATGACCTT TGTAGCCGAT TTGCAGCTGT CATGGAGTCA 
ATTCAAGGAG TGTCAGCTGT GACAGTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215873.1
CDS	87..3239
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215816.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215816.4

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA TAGCAATCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT CACGGAAGGG 
ATTCGTGAAG GAAAGCCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC CCTTCAGGTA 
AACGGTTATC GTAACTTAGA CGAATGTTTA GAAGGGGCCA TGGTGGAGGG TGACATTGAG 
CTGCTTCCTT CCGATCATTC AGTGAAGTAC GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTCAATC AGTCCCTGGG TCAGCCAGAG 
AAAATTCACA ATAAGCTTGA ATTTCCTCAG ATCATTTATA TGGACAGGTA TATGTACAGG 
AGCAAAGAGC TTGTTCGAAG TAAGCGAGAT TGTATTCGAA AACTCAAAGA AGAAATAAAA 
ATTCTGCAGC AAAAACTGGA AAGGTATGTG AAGTATGGCT CGGGCCCAGC CCGGTTCCCG 
CTTCCGGACA TGTTGAAATA CGTGATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAGAGC 
TCTGCATCTC AAAGTGATGC GTGCATGACA GAACCACTTT CTTCAGCACA CTGCCTGACT 
TCTGATCTGA CATCCAAGGA AAGTACAAGT GAAGAGAGCA CGTCTCAGGA AGCTGAAGGA 
ACCTTTTCTT CCTCTGAAGA TTCTGGACGC AAGTCTAGGA TGGCAGAGCA GCCACTTAAA 
CCTCCCCGTT CTTCCGTGGA AATGCCCGCA CACCCAGCTC CTCGAACAGT CACAGATGAG 
GAGATAAACT TTGTTAAGAC CTGCCTGCAG AGGTGGAGGA ACGAGATTGA ACAGGATATA 
CAAGACTTAA AGAACAGTAT TGCAAGCACT ACCCAGACTA TTGAGCAGAT GTACTGTGAT 
CCTCTCCTCC GTCAGGTGCC TTATCGCTTG CACGCGGTCC TCGTTCACGA AGGACAGGCA 
AACGCGGGGC ACTACTGGGC CTACGTCTAT AATCAGCCGC GACAAGTCTG GCTCAAGTAC 
AACGACATCT CTGTTACTGA GTCTTCCTGG GAAGAACTTG AAAGAGATTC CTATGGGGGC 
CAGAGAAATG TTAGTGCTTA CTGCCTGATG TATATTAACG ACAAGCTGCC CCACTTCAAT 
GCAGAGGCGG CCCCAAATGA ATTAGATCAG ATGTCAGGAG AAGTAGAAGC CCTGTCTGTT 
GAACTTAAGC ATTACATTCA GGAAGATAAC TGGAGGTTTG AGCAGGAAGT GGAGGAATGG 
GAAGAAGAGC AGTCTTGTAA AATTCCTCAG ATGGCATCTT CCACCAGCTC AGCATCACAG 
GACTTCTCTC CATCTCAAGA ATCTTCAGTA GCCTCTTCCC ACGGGGCTCG GTGCCTGTCC 
TCTGAGCACG CTGTGATCGT GAAGGAGCAG ACTGCCCAGG CGATTGCAAA CACAGCACAT 
GCCTATGAGA ACAGTGGCGT AGAAGCTGCA CTGAGTGAGG CATTCCATGA AGAGTACTCC 
AGGCTCTATC AGCTCGCCAA GGAGACCCCC ACCTCTCACA GTGATCCTCG TCTTCAGCAC 
GTGCTCATCT ACTTCTTCCA AAATGAAGCG CCTAAAAGGG TAGTAGAGCG GACCCTTCTG 
GAACAATTTG CCGATAAAAA CCTTAGCTAT GATGAAAGAT CGATCAGTAT TATGAAGGTG 
GCTCAAGCAA AACTGAAGGA GATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG 
TGGCATGAAG ATTACAGTTT GTTTCGAAAG GTGTCTGTGT ATCTGCTGAC AGGCCTGGAA 
CTCTATCAGA AAGGAAAGTA CCAAGAAGCG CTTTCCTACC TGGTGTATGC CTACCAAAGT 
AATGCCACTC TGCTGATGAA GGGGCCCCAG CGGGGCGTAA AGGAGTCGGT GATCGCTTTA 
TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTCTT TGAAACAAAT 
GATGAGCACT CTGTAACAGA GGGTATTAAT GTGATGAATG AGCTGATCAT TCCCTGTATT 
CACCTTATCA TTAATAATGA CATCTCCAAG GATGACCTGG ATGCCATTGA GGTCATGAGA 
AACCACTGGT GCTCTTACCT TGGGCAGGAT ATCGCAGAAA ATCTGCAGCT GTGCTTAGGG 
GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TTGTCTTAAA GGAGCCTCCA 
ACTATTCGGC CCAATTCTCC CTATGACCTT TGTAGCCGAT TTGCAGCTGT CATGGAGTCA 
ATTCAAGGAG TGTCAGCTGT GACAGTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215817.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215874.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa26222
Clone ID Related Accession (Same CDS sequence)	XM_005215817.3 , XM_005215817.4
Accession Version	XM_005215817.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215817.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGCAT TCCATGAAGA GTACTCCAGG 
CTCTATCAGC TCGCCAAGGA GACCCCCACC TCTCACAGTG ATCCTCGTCT TCAGCACGTG 
CTCATCTACT TCTTCCAAAA TGAAGCGCCT AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA 
CAATTTGCCG ATAAAAACCT TAGCTATGAT GAAAGATCGA TCAGTATTAT GAAGGTGGCT 
CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT GACATGAATA TGGAAGAGTA CAAGAAGTGG 
CATGAAGATT ACAGTTTGTT TCGAAAGGTG TCTGTGTATC TGCTGACAGG CCTGGAACTC 
TATCAGAAAG GAAAGTACCA AGAAGCGCTT TCCTACCTGG TGTATGCCTA CCAAAGTAAT 
GCCACTCTGC TGATGAAGGG GCCCCAGCGG GGCGTAAAGG AGTCGGTGAT CGCTTTATAC 
CGAAGAAAAT GCCTTCTGGA GCTGAATGCC AAAGCAGCTT CTCTCTTTGA AACAAATGAT 
GAGCACTCTG TAACAGAGGG TATTAATGTG ATGAATGAGC TGATCATTCC CTGTATTCAC 
CTTATCATTA ATAATGACAT CTCCAAGGAT GACCTGGATG CCATTGAGGT CATGAGAAAC 
CACTGGTGCT CTTACCTTGG GCAGGATATC GCAGAAAATC TGCAGCTGTG CTTAGGGGAG 
TTTCTACCCA GACTTCTAGA TCCTTCTGCA GAAATCATTG TCTTAAAGGA GCCTCCAACT 
ATTCGGCCCA ATTCTCCCTA TGACCTTTGT AGCCGATTTG CAGCTGTCAT GGAGTCAATT 
CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215874.1
CDS	70..3219
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215817.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215817.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGCAT TCCATGAAGA GTACTCCAGG 
CTCTATCAGC TCGCCAAGGA GACCCCCACC TCTCACAGTG ATCCTCGTCT TCAGCACGTG 
CTCATCTACT TCTTCCAAAA TGAAGCGCCT AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA 
CAATTTGCCG ATAAAAACCT TAGCTATGAT GAAAGATCGA TCAGTATTAT GAAGGTGGCT 
CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT GACATGAATA TGGAAGAGTA CAAGAAGTGG 
CATGAAGATT ACAGTTTGTT TCGAAAGGTG TCTGTGTATC TGCTGACAGG CCTGGAACTC 
TATCAGAAAG GAAAGTACCA AGAAGCGCTT TCCTACCTGG TGTATGCCTA CCAAAGTAAT 
GCCACTCTGC TGATGAAGGG GCCCCAGCGG GGCGTAAAGG AGTCGGTGAT CGCTTTATAC 
CGAAGAAAAT GCCTTCTGGA GCTGAATGCC AAAGCAGCTT CTCTCTTTGA AACAAATGAT 
GAGCACTCTG TAACAGAGGG TATTAATGTG ATGAATGAGC TGATCATTCC CTGTATTCAC 
CTTATCATTA ATAATGACAT CTCCAAGGAT GACCTGGATG CCATTGAGGT CATGAGAAAC 
CACTGGTGCT CTTACCTTGG GCAGGATATC GCAGAAAATC TGCAGCTGTG CTTAGGGGAG 
TTTCTACCCA GACTTCTAGA TCCTTCTGCA GAAATCATTG TCTTAAAGGA GCCTCCAACT 
ATTCGGCCCA ATTCTCCCTA TGACCTTTGT AGCCGATTTG CAGCTGTCAT GGAGTCAATT 
CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005215817.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215874.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa26222
Clone ID Related Accession (Same CDS sequence)	XM_005215817.3 , XM_005215817.4
Accession Version	XM_005215817.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215817.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGCAT TCCATGAAGA GTACTCCAGG 
CTCTATCAGC TCGCCAAGGA GACCCCCACC TCTCACAGTG ATCCTCGTCT TCAGCACGTG 
CTCATCTACT TCTTCCAAAA TGAAGCGCCT AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA 
CAATTTGCCG ATAAAAACCT TAGCTATGAT GAAAGATCGA TCAGTATTAT GAAGGTGGCT 
CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT GACATGAATA TGGAAGAGTA CAAGAAGTGG 
CATGAAGATT ACAGTTTGTT TCGAAAGGTG TCTGTGTATC TGCTGACAGG CCTGGAACTC 
TATCAGAAAG GAAAGTACCA AGAAGCGCTT TCCTACCTGG TGTATGCCTA CCAAAGTAAT 
GCCACTCTGC TGATGAAGGG GCCCCAGCGG GGCGTAAAGG AGTCGGTGAT CGCTTTATAC 
CGAAGAAAAT GCCTTCTGGA GCTGAATGCC AAAGCAGCTT CTCTCTTTGA AACAAATGAT 
GAGCACTCTG TAACAGAGGG TATTAATGTG ATGAATGAGC TGATCATTCC CTGTATTCAC 
CTTATCATTA ATAATGACAT CTCCAAGGAT GACCTGGATG CCATTGAGGT CATGAGAAAC 
CACTGGTGCT CTTACCTTGG GCAGGATATC GCAGAAAATC TGCAGCTGTG CTTAGGGGAG 
TTTCTACCCA GACTTCTAGA TCCTTCTGCA GAAATCATTG TCTTAAAGGA GCCTCCAACT 
ATTCGGCCCA ATTCTCCCTA TGACCTTTGT AGCCGATTTG CAGCTGTCAT GGAGTCAATT 
CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215874.1
CDS	87..3236
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTEKRNIVFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSPEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_005215817.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215817.4

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGGA AAAGAGAAAT ATCGTGTTTA TGCAGGAGCT TCAATATTTG 
TTTGCTCTGA TGATGGGATC AAATCGCAAG TTTGTCGACC CTTCAGCAGC CCTGGACCTC 
TTAAAGGGAG CGTTCCGATC CCCTGAGGAA CAGCAGCAAG ATGTGAGTGA GTTCACACAC 
AAGCTCCTGG ATTGGCTGGA GGACGCCTTC CAGCTAGCTG TTAATGTTAA CAATCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTCAC GGAAGGGATT 
CGTGAAGGAA AGCCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCCCT TCAGGTAAAC 
GGTTATCGTA ACTTAGACGA ATGTTTAGAA GGGGCCATGG TGGAGGGTGA CATTGAGCTG 
CTTCCTTCCG ATCATTCAGT GAAGTACGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTCAATCAGT CCCTGGGTCA GCCAGAGAAA 
ATTCACAATA AGCTTGAATT TCCTCAGATC ATTTATATGG ACAGGTATAT GTACAGGAGC 
AAAGAGCTTG TTCGAAGTAA GCGAGATTGT ATTCGAAAAC TCAAAGAAGA AATAAAAATT 
CTGCAGCAAA AACTGGAAAG GTATGTGAAG TATGGCTCGG GCCCAGCCCG GTTCCCGCTT 
CCGGACATGT TGAAATACGT GATTGAATTT GCTAGTACAA AACCTGCCTC AGAGAGCTCT 
GCATCTCAAA GTGATGCGTG CATGACAGAA CCACTTTCTT CAGCACACTG CCTGACTTCT 
GATCTGACAT CCAAGGAAAG TACAAGTGAA GAGAGCACGT CTCAGGAAGC TGAAGGAACC 
TTTTCTTCCT CTGAAGATTC TGGACGCAAG TCTAGGATGG CAGAGCAGCC ACTTAAACCT 
CCCCGTTCTT CCGTGGAAAT GCCCGCACAC CCAGCTCCTC GAACAGTCAC AGATGAGGAG 
ATAAACTTTG TTAAGACCTG CCTGCAGAGG TGGAGGAACG AGATTGAACA GGATATACAA 
GACTTAAAGA ACAGTATTGC AAGCACTACC CAGACTATTG AGCAGATGTA CTGTGATCCT 
CTCCTCCGTC AGGTGCCTTA TCGCTTGCAC GCGGTCCTCG TTCACGAAGG ACAGGCAAAC 
GCGGGGCACT ACTGGGCCTA CGTCTATAAT CAGCCGCGAC AAGTCTGGCT CAAGTACAAC 
GACATCTCTG TTACTGAGTC TTCCTGGGAA GAACTTGAAA GAGATTCCTA TGGGGGCCAG 
AGAAATGTTA GTGCTTACTG CCTGATGTAT ATTAACGACA AGCTGCCCCA CTTCAATGCA 
GAGGCGGCCC CAAATGAATT AGATCAGATG TCAGGAGAAG TAGAAGCCCT GTCTGTTGAA 
CTTAAGCATT ACATTCAGGA AGATAACTGG AGGTTTGAGC AGGAAGTGGA GGAATGGGAA 
GAAGAGCAGT CTTGTAAAAT TCCTCAGATG GCATCTTCCA CCAGCTCAGC ATCACAGGAC 
TTCTCTCCAT CTCAAGAATC TTCAGTAGCC TCTTCCCACG GGGCTCGGTG CCTGTCCTCT 
GAGCACGCTG TGATCGTGAA GGAGCAGACT GCCCAGGCGA TTGCAAACAC AGCACATGCC 
TATGAGAACA GTGGCGTAGA AGCTGCACTG AGTGAGGCAT TCCATGAAGA GTACTCCAGG 
CTCTATCAGC TCGCCAAGGA GACCCCCACC TCTCACAGTG ATCCTCGTCT TCAGCACGTG 
CTCATCTACT TCTTCCAAAA TGAAGCGCCT AAAAGGGTAG TAGAGCGGAC CCTTCTGGAA 
CAATTTGCCG ATAAAAACCT TAGCTATGAT GAAAGATCGA TCAGTATTAT GAAGGTGGCT 
CAAGCAAAAC TGAAGGAGAT TGGTCCAGAT GACATGAATA TGGAAGAGTA CAAGAAGTGG 
CATGAAGATT ACAGTTTGTT TCGAAAGGTG TCTGTGTATC TGCTGACAGG CCTGGAACTC 
TATCAGAAAG GAAAGTACCA AGAAGCGCTT TCCTACCTGG TGTATGCCTA CCAAAGTAAT 
GCCACTCTGC TGATGAAGGG GCCCCAGCGG GGCGTAAAGG AGTCGGTGAT CGCTTTATAC 
CGAAGAAAAT GCCTTCTGGA GCTGAATGCC AAAGCAGCTT CTCTCTTTGA AACAAATGAT 
GAGCACTCTG TAACAGAGGG TATTAATGTG ATGAATGAGC TGATCATTCC CTGTATTCAC 
CTTATCATTA ATAATGACAT CTCCAAGGAT GACCTGGATG CCATTGAGGT CATGAGAAAC 
CACTGGTGCT CTTACCTTGG GCAGGATATC GCAGAAAATC TGCAGCTGTG CTTAGGGGAG 
TTTCTACCCA GACTTCTAGA TCCTTCTGCA GAAATCATTG TCTTAAAGGA GCCTCCAACT 
ATTCGGCCCA ATTCTCCCTA TGACCTTTGT AGCCGATTTG CAGCTGTCAT GGAGTCAATT 
CAAGGAGTGT CAGCTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_010812360.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810662.1 Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

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CloneID	OBa47296
Clone ID Related Accession (Same CDS sequence)	XM_010812360.2
Accession Version	XM_010812360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGCA AGATGTGAGT GAGTTCACAC ACAAGCTCCT GGATTGGCTG 
GAGGACGCCT TCCAGCTAGC TGTTAATGTT AATAGCAATC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTCACGGAAG GGATTCGTGA AGGAAAGCCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCCCTTCAGG TAAACGGTTA TCGTAACTTA 
GACGAATGTT TAGAAGGGGC CATGGTGGAG GGTGACATTG AGCTGCTTCC TTCCGATCAT 
TCAGTGAAGT ACGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTCAA TCAGTCCCTG GGTCAGCCAG AGAAAATTCA CAATAAGCTT 
GAATTTCCTC AGATCATTTA TATGGACAGG TATATGTACA GGAGCAAAGA GCTTGTTCGA 
AGTAAGCGAG ATTGTATTCG AAAACTCAAA GAAGAAATAA AAATTCTGCA GCAAAAACTG 
GAAAGGTATG TGAAGTATGG CTCGGGCCCA GCCCGGTTCC CGCTTCCGGA CATGTTGAAA 
TACGTGATTG AATTTGCTAG TACAAAACCT GCCTCAGAGA GCTCTGCATC TCAAAGTGAT 
GCGTGCATGA CAGAACCACT TTCTTCAGCA CACTGCCTGA CTTCTGATCT GACATCCAAG 
GAAAGTACAA GTGAAGAGAG CACGTCTCAG GAAGCTGAAG GAACCTTTTC TTCCTCTGAA 
GATTCTGGAC GCAAGTCTAG GATGGCAGAG CAGCCACTTA AACCTCCCCG TTCTTCCGTG 
GAAATGCCCG CACACCCAGC TCCTCGAACA GTCACAGATG AGGAGATAAA CTTTGTTAAG 
ACCTGCCTGC AGAGGTGGAG GAACGAGATT GAACAGGATA TACAAGACTT AAAGAACAGT 
ATTGCAAGCA CTACCCAGAC TATTGAGCAG ATGTACTGTG ATCCTCTCCT CCGTCAGGTG 
CCTTATCGCT TGCACGCGGT CCTCGTTCAC GAAGGACAGG CAAACGCGGG GCACTACTGG 
GCCTACGTCT ATAATCAGCC GCGACAAGTC TGGCTCAAGT ACAACGACAT CTCTGTTACT 
GAGTCTTCCT GGGAAGAACT TGAAAGAGAT TCCTATGGGG GCCAGAGAAA TGTTAGTGCT 
TACTGCCTGA TGTATATTAA CGACAAGCTG CCCCACTTCA ATGCAGAGGC GGCCCCAAAT 
GAATTAGATC AGATGTCAGG AGAAGTAGAA GCCCTGTCTG TTGAACTTAA GCATTACATT 
CAGGAAGATA ACTGGAGGTT TGAGCAGGAA GTGGAGGAAT GGGAAGAAGA GCAGTCTTGT 
AAAATTCCTC AGATGGCATC TTCCACCAGC TCAGCATCAC AGGACTTCTC TCCATCTCAA 
GAATCTTCAG TAGCCTCTTC CCACGGGGCT CGGTGCCTGT CCTCTGAGCA CGCTGTGATC 
GTGAAGGAGC AGACTGCCCA GGCGATTGCA AACACAGCAC ATGCCTATGA GAACAGTGGC 
GTAGAAGCTG CACTGAGTGA GCTTAAGGAA GCTGAACCCA AGAAGCCCAT GCCCCCGGAA 
ACAAACCTTG CAGAGCAGTC AGAGCAGCCC CCACAGGCTC GTGACGCAGA GTCTGCTGCC 
CAGCCTAATG CTGAGGTCTC TGAAGTCGAG ATTCCCAGTG TGGGAAGGAT TCTGGTTAGA 
TCTGATGCAG ATGGATATGA TGAGGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC 
CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTTATT 
AAGGCATTCC ATGAAGAGTA CTCCAGGCTC TATCAGCTCG CCAAGGAGAC CCCCACCTCT 
CACAGTGATC CTCGTCTTCA GCACGTGCTC ATCTACTTCT TCCAAAATGA AGCGCCTAAA 
AGGGTAGTAG AGCGGACCCT TCTGGAACAA TTTGCCGATA AAAACCTTAG CTATGATGAA 
AGATCGATCA GTATTATGAA GGTGGCTCAA GCAAAACTGA AGGAGATTGG TCCAGATGAC 
ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTACA GTTTGTTTCG AAAGGTGTCT 
GTGTATCTGC TGACAGGCCT GGAACTCTAT CAGAAAGGAA AGTACCAAGA AGCGCTTTCC 
TACCTGGTGT ATGCCTACCA AAGTAATGCC ACTCTGCTGA TGAAGGGGCC CCAGCGGGGC 
GTAAAGGAGT CGGTGATCGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA 
GCAGCTTCTC TCTTTGAAAC AAATGATGAG CACTCTGTAA CAGAGGGTAT TAATGTGATG 
AATGAGCTGA TCATTCCCTG TATTCACCTT ATCATTAATA ATGACATCTC CAAGGATGAC 
CTGGATGCCA TTGAGGTCAT GAGAAACCAC TGGTGCTCTT ACCTTGGGCA GGATATCGCA 
GAAAATCTGC AGCTGTGCTT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA 
ATCATTGTCT TAAAGGAGCC TCCAACTATT CGGCCCAATT CTCCCTATGA CCTTTGTAGC 
CGATTTGCAG CTGTCATGGA GTCAATTCAA GGAGTGTCAG CTGTGACAGT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810662.1
CDS	70..3366
Translation	MTAELQQDDAAGATDRHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQETAAEPSEEEGSAASKEELAKVIDLTHDSKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCPSHTQDVSEFTHKLLDWLEDAFQLAVNVNSNPRNKSENPMVQLFYGTFLTEGIREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDIELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELVRSKRDCIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESSASQSDACMTEPLSSAHCLTSDLTSKESTSEESTSQEAEGTFSSSEDSGRKSRMAEQPLKPPRSSVEMPAHPAPRTVTDEEINFVKTCLQRWRNEIEQDIQDLKNSIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYVYNQPRQVWLKYNDISVTESSWEELERDSYGGQRNVSAYCLMYINDKLPHFNAEAAPNELDQMSGEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMASSTSSASQDFSPSQESSVASSHGARCLSSEHAVIVKEQTAQAIANTAHAYENSGVEAALSELKEAEPKKPMPPETNLAEQSEQPPQARDAESAAQPNAEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLIYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNATLLMKGPQRGVKESVIALYRRKCLLELNAKAASLFETNDEHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSAVTVK

Target ORF information:

RefSeq Version	XM_010812360.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812360.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGAGCGA CCGACCGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCTTTTCTT 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATCACCCAGG CCGTCAGCCT TCTCACTGAT 
GAAAGAGTTA AAGAGCCCAG CCAAGAGACT GCTGCAGAAC CATCTGAAGA AGAGGGGAGT 
GCTGCCAGCA AAGAGGAATT AGCAAAAGTG ATAGACCTTA CTCACGATAG CAAGGATGAT 
CTTCAGGCTG CCATTGCTTT GAGTCTGCTG GAGTCCCCCA AAATTCAAGC TGATGGAAGA 
GATCTTAACA GGATGCACGA GGCAACCTCT GCAGAAACTA AACGCTCAAA GAGAAAACGC 
TGTGAAGTCT GGGGAGAAAA TCCCAATCCC AATGACTGGA GACGAGTTGA TGGTTGGCCA 
GTTGGGCTGA AAAATGTTGG CAATACATGT TGGTTTAGTG CTGTTATTCA GTCTCTGTTT 
CAATTGCCTG AATTCCGAAG ACTTGTCCTC AGCTATAGTC TACCACAGAA CGTACTTGAA 
AATTGTCCAA GTCACACGCA AGATGTGAGT GAGTTCACAC ACAAGCTCCT GGATTGGCTG 
GAGGACGCCT TCCAGCTAGC TGTTAATGTT AATAGCAATC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTCACGGAAG GGATTCGTGA AGGAAAGCCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCCCTTCAGG TAAACGGTTA TCGTAACTTA 
GACGAATGTT TAGAAGGGGC CATGGTGGAG GGTGACATTG AGCTGCTTCC TTCCGATCAT 
TCAGTGAAGT ACGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTCAA TCAGTCCCTG GGTCAGCCAG AGAAAATTCA CAATAAGCTT 
GAATTTCCTC AGATCATTTA TATGGACAGG TATATGTACA GGAGCAAAGA GCTTGTTCGA 
AGTAAGCGAG ATTGTATTCG AAAACTCAAA GAAGAAATAA AAATTCTGCA GCAAAAACTG 
GAAAGGTATG TGAAGTATGG CTCGGGCCCA GCCCGGTTCC CGCTTCCGGA CATGTTGAAA 
TACGTGATTG AATTTGCTAG TACAAAACCT GCCTCAGAGA GCTCTGCATC TCAAAGTGAT 
GCGTGCATGA CAGAACCACT TTCTTCAGCA CACTGCCTGA CTTCTGATCT GACATCCAAG 
GAAAGTACAA GTGAAGAGAG CACGTCTCAG GAAGCTGAAG GAACCTTTTC TTCCTCTGAA 
GATTCTGGAC GCAAGTCTAG GATGGCAGAG CAGCCACTTA AACCTCCCCG TTCTTCCGTG 
GAAATGCCCG CACACCCAGC TCCTCGAACA GTCACAGATG AGGAGATAAA CTTTGTTAAG 
ACCTGCCTGC AGAGGTGGAG GAACGAGATT GAACAGGATA TACAAGACTT AAAGAACAGT 
ATTGCAAGCA CTACCCAGAC TATTGAGCAG ATGTACTGTG ATCCTCTCCT CCGTCAGGTG 
CCTTATCGCT TGCACGCGGT CCTCGTTCAC GAAGGACAGG CAAACGCGGG GCACTACTGG 
GCCTACGTCT ATAATCAGCC GCGACAAGTC TGGCTCAAGT ACAACGACAT CTCTGTTACT 
GAGTCTTCCT GGGAAGAACT TGAAAGAGAT TCCTATGGGG GCCAGAGAAA TGTTAGTGCT 
TACTGCCTGA TGTATATTAA CGACAAGCTG CCCCACTTCA ATGCAGAGGC GGCCCCAAAT 
GAATTAGATC AGATGTCAGG AGAAGTAGAA GCCCTGTCTG TTGAACTTAA GCATTACATT 
CAGGAAGATA ACTGGAGGTT TGAGCAGGAA GTGGAGGAAT GGGAAGAAGA GCAGTCTTGT 
AAAATTCCTC AGATGGCATC TTCCACCAGC TCAGCATCAC AGGACTTCTC TCCATCTCAA 
GAATCTTCAG TAGCCTCTTC CCACGGGGCT CGGTGCCTGT CCTCTGAGCA CGCTGTGATC 
GTGAAGGAGC AGACTGCCCA GGCGATTGCA AACACAGCAC ATGCCTATGA GAACAGTGGC 
GTAGAAGCTG CACTGAGTGA GCTTAAGGAA GCTGAACCCA AGAAGCCCAT GCCCCCGGAA 
ACAAACCTTG CAGAGCAGTC AGAGCAGCCC CCACAGGCTC GTGACGCAGA GTCTGCTGCC 
CAGCCTAATG CTGAGGTCTC TGAAGTCGAG ATTCCCAGTG TGGGAAGGAT TCTGGTTAGA 
TCTGATGCAG ATGGATATGA TGAGGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC 
CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTTATT 
AAGGCATTCC ATGAAGAGTA CTCCAGGCTC TATCAGCTCG CCAAGGAGAC CCCCACCTCT 
CACAGTGATC CTCGTCTTCA GCACGTGCTC ATCTACTTCT TCCAAAATGA AGCGCCTAAA 
AGGGTAGTAG AGCGGACCCT TCTGGAACAA TTTGCCGATA AAAACCTTAG CTATGATGAA 
AGATCGATCA GTATTATGAA GGTGGCTCAA GCAAAACTGA AGGAGATTGG TCCAGATGAC 
ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTACA GTTTGTTTCG AAAGGTGTCT 
GTGTATCTGC TGACAGGCCT GGAACTCTAT CAGAAAGGAA AGTACCAAGA AGCGCTTTCC 
TACCTGGTGT ATGCCTACCA AAGTAATGCC ACTCTGCTGA TGAAGGGGCC CCAGCGGGGC 
GTAAAGGAGT CGGTGATCGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA 
GCAGCTTCTC TCTTTGAAAC AAATGATGAG CACTCTGTAA CAGAGGGTAT TAATGTGATG 
AATGAGCTGA TCATTCCCTG TATTCACCTT ATCATTAATA ATGACATCTC CAAGGATGAC 
CTGGATGCCA TTGAGGTCAT GAGAAACCAC TGGTGCTCTT ACCTTGGGCA GGATATCGCA 
GAAAATCTGC AGCTGTGCTT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA 
ATCATTGTCT TAAAGGAGCC TCCAACTATT CGGCCCAATT CTCCCTATGA CCTTTGTAGC 
CGATTTGCAG CTGTCATGGA GTCAATTCAA GGAGTGTCAG CTGTGACAGT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	USP28
Entrez Gene ID	508902
Full Name	ubiquitin specific peptidase 28
General protein information	Preferred Names ubiquitin specific peptidase 28 Names ubiquitin carboxyl-terminal hydrolase 28
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Rattus norvegicus (Norway rat)	Usp28	NP_001101614.1
Danio rerio (zebrafish)	usp28	XP_001920096.1
Xenopus tropicalis (tropical clawed frog)	usp28	XP_004916150.1
Canis lupus familiaris (dog)	USP28	XP_005619813.1
Mus musculus (house mouse)	Usp28	NP_780691.2
Pan troglodytes (chimpanzee)	USP28	XP_003313357.2
	USP28	XP_002799842.1
Bos taurus (cattle)	USP28	NP_001179927.1
Homo sapiens (human)	USP28	NP_065937.1
Gallus gallus (chicken)	USP28	NP_001026667.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

USP28 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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