USP28 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP28
Entrez Gene ID	57646
Full Name	ubiquitin specific peptidase 28
General protein information	Preferred Names ubiquitin specific peptidase 28 Names ubiquitin carboxyl-terminal hydrolase 28 deubiquitinating enzyme 28 ubiquitin carboxyl-terminal hydrolase 28 variant 1 ubiquitin thioesterase 28 ubiquitin thiolesterase 28 ubiquitin-specific-processing protease 28
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q23.2
Summary	The protein encoded by this gene is a deubiquitinase involved in the DNA damage pathway and DNA damage-induced apoptosis. Overexpression of this gene is seen in several cancers. [provided by RefSeq, Oct 2016].
Disorder MIM:	610748

mRNA and Protein(s)

mRNA	Protein	Name
XM_005271636.2	XP_005271693.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X7
NM_001346259.1	NP_001333188.1	ubiquitin carboxyl-terminal hydrolase 28 isoform i
NM_001301029.1	NP_001287958.1	ubiquitin carboxyl-terminal hydrolase 28 isoform b
NM_001346270.1	NP_001333199.1	ubiquitin carboxyl-terminal hydrolase 28 isoform p
NM_001346269.1	NP_001333198.1	ubiquitin carboxyl-terminal hydrolase 28 isoform p
NM_001346262.2	NP_001333191.1	ubiquitin carboxyl-terminal hydrolase 28 isoform b
NM_001346254.2	NP_001333183.1	ubiquitin carboxyl-terminal hydrolase 28 isoform e
NM_001346268.2	NP_001333197.1	ubiquitin carboxyl-terminal hydrolase 28 isoform o
NM_001346272.2	NP_001333201.1	ubiquitin carboxyl-terminal hydrolase 28 isoform q
NM_001346255.1	NP_001333184.1	ubiquitin carboxyl-terminal hydrolase 28 isoform f
XM_005271632.2	XP_005271689.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
XM_011542936.2	XP_011541238.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
XM_017018060.1	XP_016873549.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X15
XM_017018069.1	XP_016873558.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X24
NM_001346263.1	NP_001333192.1	ubiquitin carboxyl-terminal hydrolase 28 isoform l
NM_001346271.1	NP_001333200.1	ubiquitin carboxyl-terminal hydrolase 28 isoform p
NM_001346264.1	NP_001333193.1	ubiquitin carboxyl-terminal hydrolase 28 isoform m
NM_001346273.1	NP_001333202.1	ubiquitin carboxyl-terminal hydrolase 28 isoform r
NM_001346267.2	NP_001333196.1	ubiquitin carboxyl-terminal hydrolase 28 isoform o
NM_001346261.2	NP_001333190.1	ubiquitin carboxyl-terminal hydrolase 28 isoform k
NM_001346259.2	NP_001333188.1	ubiquitin carboxyl-terminal hydrolase 28 isoform i
NM_001301029.2	NP_001287958.1	ubiquitin carboxyl-terminal hydrolase 28 isoform b
NM_001346252.2	NP_001333181.1	ubiquitin carboxyl-terminal hydrolase 28 isoform c
NM_001346271.2	NP_001333200.1	ubiquitin carboxyl-terminal hydrolase 28 isoform p
NM_001346270.2	NP_001333199.1	ubiquitin carboxyl-terminal hydrolase 28 isoform p
XM_011542938.1	XP_011541240.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X14
XM_017018064.1	XP_016873553.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X19
NM_001346258.1	NP_001333187.1	ubiquitin carboxyl-terminal hydrolase 28 isoform h
NM_001346261.1	NP_001333190.1	ubiquitin carboxyl-terminal hydrolase 28 isoform k
NM_001346267.1	NP_001333196.1	ubiquitin carboxyl-terminal hydrolase 28 isoform o
NM_020886.4	NP_065937.1	ubiquitin carboxyl-terminal hydrolase 28 isoform a
NM_001346253.2	NP_001333182.1	ubiquitin carboxyl-terminal hydrolase 28 isoform d
NM_001346273.2	NP_001333202.1	ubiquitin carboxyl-terminal hydrolase 28 isoform r
NM_001346264.2	NP_001333193.1	ubiquitin carboxyl-terminal hydrolase 28 isoform m
NM_001346265.2	NP_001333194.1	ubiquitin carboxyl-terminal hydrolase 28 isoform n
NM_001346260.2	NP_001333189.1	ubiquitin carboxyl-terminal hydrolase 28 isoform j
NM_001346272.1	NP_001333201.1	ubiquitin carboxyl-terminal hydrolase 28 isoform q
NM_001346253.1	NP_001333182.1	ubiquitin carboxyl-terminal hydrolase 28 isoform d
NM_001346252.1	NP_001333181.1	ubiquitin carboxyl-terminal hydrolase 28 isoform c
XM_005271638.2	XP_005271695.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X11
XM_017018067.1	XP_016873556.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X21
XM_011542942.1	XP_011541244.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X22
NM_020886.3	NP_065937.1	ubiquitin carboxyl-terminal hydrolase 28 isoform a
NM_001346262.1	NP_001333191.1	ubiquitin carboxyl-terminal hydrolase 28 isoform b
NM_001346255.2	NP_001333184.1	ubiquitin carboxyl-terminal hydrolase 28 isoform f
NM_001346254.1	NP_001333183.1	ubiquitin carboxyl-terminal hydrolase 28 isoform e
NM_001346260.1	NP_001333189.1	ubiquitin carboxyl-terminal hydrolase 28 isoform j
NM_001346265.1	NP_001333194.1	ubiquitin carboxyl-terminal hydrolase 28 isoform n
XM_005271633.4	XP_005271690.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
XM_005271630.2	XP_005271687.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
NM_001346257.1	NP_001333186.1	ubiquitin carboxyl-terminal hydrolase 28 isoform g
NM_001346268.1	NP_001333197.1	ubiquitin carboxyl-terminal hydrolase 28 isoform o
NM_001346258.2	NP_001333187.1	ubiquitin carboxyl-terminal hydrolase 28 isoform h
NM_001346269.2	NP_001333198.1	ubiquitin carboxyl-terminal hydrolase 28 isoform p
NM_001346257.2	NP_001333186.1	ubiquitin carboxyl-terminal hydrolase 28 isoform g
NM_001346263.2	NP_001333192.1	ubiquitin carboxyl-terminal hydrolase 28 isoform l

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Usp28	NP_001101614.1
Danio rerio (zebrafish)	usp28	XP_001920096.1
Xenopus tropicalis (tropical clawed frog)	usp28	XP_004916150.1
Canis lupus familiaris (dog)	USP28	XP_005619813.1
Mus musculus (house mouse)	Usp28	NP_780691.2
Pan troglodytes (chimpanzee)	USP28	XP_003313357.2
	USP28	XP_002799842.1
Bos taurus (cattle)	USP28	NP_001179927.1
Homo sapiens (human)	USP28	NP_065937.1
Gallus gallus (chicken)	USP28	NP_001026667.1

Gene Ontology

The following USP28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu100648	NM_001346273.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu45852	NM_001346262.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu45852	NM_001301029.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu70770	NM_001346263.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu70771	NM_001346265.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu70772	XM_011542942.1 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu100563	NM_001346261.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100589	NM_001346272.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100612	NM_001346267.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100612	NM_001346268.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100639	NM_001346264.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100689	NM_001346260.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu92401	NM_001346270.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu92401	NM_001346271.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu92401	NM_001346269.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu92400	XM_017018064.1 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu92401	XM_017018067.1 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu92402	XM_017018069.1 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu100535	NM_001346259.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100560	NM_001346255.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100584	NM_001346253.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu100646	NM_001346257.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu92399	XM_017018060.1 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu03574	NM_020886.4 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$762.30 $1089.00
OHu45845	NM_001346252.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu45849	NM_001346254.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu45851	NM_001346258.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu45844	XM_005271630.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu45846	XM_005271632.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu45847	XM_005271633.4 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu45848	XM_005271636.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu45850	XM_005271638.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu70766	XM_011542936.2 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu70768	XM_011542938.1 Latest version!	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu100535	NM_001346259.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu45852	NM_001301029.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu92401	NM_001346271.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu92401	NM_001346270.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100563	NM_001346261.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100612	NM_001346267.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100648	NM_001346273.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu100639	NM_001346264.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100589	NM_001346272.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100584	NM_001346253.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu45845	NM_001346252.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu03574	NM_020886.3	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$762.30 $1089.00
OHu45852	NM_001346262.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu100560	NM_001346255.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu45849	NM_001346254.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu100689	NM_001346260.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu70771	NM_001346265.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu100612	NM_001346268.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu45851	NM_001346258.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu92401	NM_001346269.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100646	NM_001346257.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu70770	NM_001346263.1	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP28 ( NM_001346273.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333202.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 22, mRNA.

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CloneID	OHu100648
Clone ID Related Accession (Same CDS sequence)	NM_001346273.1 , NM_001346273.2
Accession Version	NM_001346273.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform r
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB061316.1 and BC065928.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065928.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333202.1
CDS	70..1821
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVE

Target ORF information:

RefSeq Version	NM_001346273.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346273.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346273.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333202.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 22, mRNA.

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CloneID	OHu100648
Clone ID Related Accession (Same CDS sequence)	NM_001346273.1 , NM_001346273.2
Accession Version	NM_001346273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform r
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, DB061316.1 and BC065928.1. On Jun 2, 2019 this sequence version replaced NM_001346273.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065928.1, SRR5189655.111634.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333202.1
CDS	72..1823
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVE

Target ORF information:

RefSeq Version	NM_001346273.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346273.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001301029.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287958.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 2, mRNA.

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CloneID	OHu45852
Clone ID Related Accession (Same CDS sequence)	NM_001301029.1 , NM_001346262.2 , NM_001301029.2 , NM_001346262.1
Accession Version	NM_001301029.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB061316.1, AK304770.1, AP003170.4, BC035986.1 and BF434481.1. On Jul 18, 2014 this sequence version replaced XM_005271641.2. Transcript Variant: This variant (2), as well as variant 12, encodes isoform b (also known as isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.232651.1, SRR1803615.272582.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287958.1
CDS	234..2996
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001301029.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301029.1

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346262.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333191.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu45852
Clone ID Related Accession (Same CDS sequence)	NM_001301029.1 , NM_001346262.2 , NM_001301029.2 , NM_001346262.1
Accession Version	NM_001346262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346262.1. Transcript Variant: This variant (12), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.86542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333191.1
CDS	314..3076
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346262.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346262.2

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001301029.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287958.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu45852
Clone ID Related Accession (Same CDS sequence)	NM_001301029.1 , NM_001346262.2 , NM_001301029.2 , NM_001346262.1
Accession Version	NM_001301029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, DB061316.1, AK304770.1, AP003170.4, BC035986.1 and BF434481.1. On Jun 2, 2019 this sequence version replaced NM_001301029.1. Transcript Variant: This variant (2), as well as variant 12, encodes isoform b (also known as isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.232651.1, SRR1660803.83390.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287958.1
CDS	236..2998
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001301029.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301029.2

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346262.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333191.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu45852
Clone ID Related Accession (Same CDS sequence)	NM_001301029.1 , NM_001346262.2 , NM_001301029.2 , NM_001346262.1
Accession Version	NM_001346262.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. Transcript Variant: This variant (12), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.86542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333191.1
CDS	312..3074
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346262.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346262.1

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346263.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333192.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu70770
Clone ID Related Accession (Same CDS sequence)	NM_001346263.1 , NM_001346263.2
Accession Version	NM_001346263.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform l
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018063.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.33802.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAGC TGTTCTATGG TACTTTCCTG ACTGAAGGGG TTCGTGAAGG AAAACCCTTT 
TGTAACAATG AGACCTTCGG CCAGTATCCT CTTCAGGTAA ACGGTTATCG CAACTTAGAC 
GAGTGTTTGG AAGGGGCCAT GGTGGAGGGT GATGTTGAGC TTCTTCCCTC CGATCACTCG 
GTGAAGTATG GACAAGAGCG TTGGTTTACA AAGCTACCTC CAGTGTTGAC CTTTGAACTC 
TCAAGATTTG AGTTTAATCA GTCCCTTGGG CAGCCAGAGA AAATTCACAA TAAGCTGGAA 
TTTCCTCAGA TTATTTATAT GGACAGGTAC ATGTACAGGA GCAAGGAGCT TATTCGAAAT 
AAGAGAGAGT GTATTCGAAA GTTGAAGGAG GAAATAAAAA TTCTGCAGCA AAAATTGGAA 
AGGTATGTGA AATATGGCTC AGGCCCAGCT CGGTTCCCGC TCCCGGACAT GCTGAAATAT 
GTTATTGAAT TTGCTAGTAC AAAACCTGCC TCAGAAAGCT GTCCACCTGA AAGTGACACA 
CATATGACAT TACCACTTTC TTCAGTGCAC TGCTCGGTTT CTGACCAGAC ATCCAAGGAA 
AGTACAAGTA CAGAAAGCTC TTCTCAGGAT GTTGAAAGTA CCTTTTCTTC TCCTGAAGAT 
TCTTTACCCA AGTCTAAACC ACTGACATCT TCTCGGTCTT CCATGGAAAT GCCTTCACAG 
CCAGCTCCAC GAACAGTCAC AGATGAGGAG ATAAATTTTG TTAAGACCTG TCTTCAGAGA 
TGGAGGAGTG AGATTGAACA AGATATACAA GATTTAAAGA CTTGTATTGC AAGTACTACT 
CAGACTATTG AACAGATGTA CTGCGATCCT CTCCTTCGTC AGGTGCCTTA TCGCTTGCAT 
GCAGTTCTTG TTCATGAAGG ACAAGCAAAT GCTGGACACT ATTGGGCCTA TATCTATAAT 
CAACCCCGAC AGAGCTGGCT CAAGTACAAT GACATCTCTG TTACTGAATC TTCCTGGGAA 
GAAGTTGAAA GAGATTCCTA TGGAGGCCTG AGAAATGTTA GTGCTTACTG TCTGATGTAC 
ATTAATGACA AACTACCCTA CTTCAATGCA GAGGCAGCCC CAACTGAATC AGATCAAATG 
TCAGAAGTGG AAGCCCTATC TGTGGAACTC AAGCATTACA TTCAGGAGGA TAACTGGCGG 
TTTGAGCAGG AAGTAGAGGA GTGGGAAGAA GAGCAGTCTT GCAAAATCCC TCAAATGGAG 
TCCTCCACCA ACTCCTCATC ACAGGACTAC TCTACATCAC AAGAGCCTTC AGTAGCCTCT 
TCTCATGGGG TTCGCTGCTT GTCGTCTGAG CATGCTGTGA TTGTAAAGGA GCAAACTGCC 
CAGGCTATTG CAAACACAGC CCGTGCCTAT GAGAAGAGCG GTGTAGAAGC GGCACTGAGT 
GAGCTTAAAG AAGCTGAACC CAAGAAGCCC ATGCCCCAGG AAACAAACCT TGCAGAGCAG 
TCAGAACAGC CCCCAAAGGC TAATGATGCA GAGTCTACTG CCCAGCCTAA TTCTGAGGTC 
TCTGAAGTCG AGATTCCCAG TGTGGGAAGG ATTCTGGTTA GATCTGATGC AGATGGATAT 
GATGAGGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTGA TTAAGGCATT CCATGAAGAA 
TACTCCAGGC TCTATCAGCT TGCCAAAGAG ACCCCCACCT CTCACAGTGA TCCTCGACTT 
CAGCATGTCC TTGTCTACTT TTTCCAAAAT GAAGCACCCA AAAGGGTAGT AGAACGAACC 
CTTCTGGAAC AGTTTGCAGA TAAAAATCTT AGCTATGATG AAAGATCAAT CAGCATTATG 
AAGGTGGCTC AAGCGAAACT GAAGGAAATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA TAGTTTGTTC CGAAAAGTGT CTGTGTATCT CCTAACAGGC 
CTAGAACTCT ATCAAAAAGG AAAGTACCAA GAGGCACTTT CCTACCTGGT ATATGCCTAC 
CAGAGCAATG CTGCCCTGCT GATGAAGGGG CCCCGCCGGG GGGTCAAAGA ATCCGTGATT 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTTTTTGAA 
ACAAATGATG ATCACTCCGT AACTGAGGGC ATTAATGTGA TGAATGAACT GATCATCCCC 
TGCATTCACC TTATCATTAA TAATGACATT TCCAAGGATG ATCTGGATGC CATTGAGGTC 
ATGAGAAACC ATTGGTGCTC TTACCTTGGG CAAGATATTG CAGAAAATCT GCAGCTGTGC 
CTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATCGT CTTGAAAGAG 
CCTCCAACTA TTCGACCCAA TTCTCCCTAT GACCTATGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AGGGAGTTTC AACTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333192.1
CDS	857..3415
Translation	MVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346263.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346263.1

ORF Insert Sequence:

ATGGTGCAGC TGTTCTATGG TACTTTCCTG ACTGAAGGGG TTCGTGAAGG AAAACCCTTT 
TGTAACAATG AGACCTTCGG CCAGTATCCT CTTCAGGTAA ACGGTTATCG CAACTTAGAC 
GAGTGTTTGG AAGGGGCCAT GGTGGAGGGT GATGTTGAGC TTCTTCCCTC CGATCACTCG 
GTGAAGTATG GACAAGAGCG TTGGTTTACA AAGCTACCTC CAGTGTTGAC CTTTGAACTC 
TCAAGATTTG AGTTTAATCA GTCCCTTGGG CAGCCAGAGA AAATTCACAA TAAGCTGGAA 
TTTCCTCAGA TTATTTATAT GGACAGGTAC ATGTACAGGA GCAAGGAGCT TATTCGAAAT 
AAGAGAGAGT GTATTCGAAA GTTGAAGGAG GAAATAAAAA TTCTGCAGCA AAAATTGGAA 
AGGTATGTGA AATATGGCTC AGGCCCAGCT CGGTTCCCGC TCCCGGACAT GCTGAAATAT 
GTTATTGAAT TTGCTAGTAC AAAACCTGCC TCAGAAAGCT GTCCACCTGA AAGTGACACA 
CATATGACAT TACCACTTTC TTCAGTGCAC TGCTCGGTTT CTGACCAGAC ATCCAAGGAA 
AGTACAAGTA CAGAAAGCTC TTCTCAGGAT GTTGAAAGTA CCTTTTCTTC TCCTGAAGAT 
TCTTTACCCA AGTCTAAACC ACTGACATCT TCTCGGTCTT CCATGGAAAT GCCTTCACAG 
CCAGCTCCAC GAACAGTCAC AGATGAGGAG ATAAATTTTG TTAAGACCTG TCTTCAGAGA 
TGGAGGAGTG AGATTGAACA AGATATACAA GATTTAAAGA CTTGTATTGC AAGTACTACT 
CAGACTATTG AACAGATGTA CTGCGATCCT CTCCTTCGTC AGGTGCCTTA TCGCTTGCAT 
GCAGTTCTTG TTCATGAAGG ACAAGCAAAT GCTGGACACT ATTGGGCCTA TATCTATAAT 
CAACCCCGAC AGAGCTGGCT CAAGTACAAT GACATCTCTG TTACTGAATC TTCCTGGGAA 
GAAGTTGAAA GAGATTCCTA TGGAGGCCTG AGAAATGTTA GTGCTTACTG TCTGATGTAC 
ATTAATGACA AACTACCCTA CTTCAATGCA GAGGCAGCCC CAACTGAATC AGATCAAATG 
TCAGAAGTGG AAGCCCTATC TGTGGAACTC AAGCATTACA TTCAGGAGGA TAACTGGCGG 
TTTGAGCAGG AAGTAGAGGA GTGGGAAGAA GAGCAGTCTT GCAAAATCCC TCAAATGGAG 
TCCTCCACCA ACTCCTCATC ACAGGACTAC TCTACATCAC AAGAGCCTTC AGTAGCCTCT 
TCTCATGGGG TTCGCTGCTT GTCGTCTGAG CATGCTGTGA TTGTAAAGGA GCAAACTGCC 
CAGGCTATTG CAAACACAGC CCGTGCCTAT GAGAAGAGCG GTGTAGAAGC GGCACTGAGT 
GAGCTTAAAG AAGCTGAACC CAAGAAGCCC ATGCCCCAGG AAACAAACCT TGCAGAGCAG 
TCAGAACAGC CCCCAAAGGC TAATGATGCA GAGTCTACTG CCCAGCCTAA TTCTGAGGTC 
TCTGAAGTCG AGATTCCCAG TGTGGGAAGG ATTCTGGTTA GATCTGATGC AGATGGATAT 
GATGAGGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTGA TTAAGGCATT CCATGAAGAA 
TACTCCAGGC TCTATCAGCT TGCCAAAGAG ACCCCCACCT CTCACAGTGA TCCTCGACTT 
CAGCATGTCC TTGTCTACTT TTTCCAAAAT GAAGCACCCA AAAGGGTAGT AGAACGAACC 
CTTCTGGAAC AGTTTGCAGA TAAAAATCTT AGCTATGATG AAAGATCAAT CAGCATTATG 
AAGGTGGCTC AAGCGAAACT GAAGGAAATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA TAGTTTGTTC CGAAAAGTGT CTGTGTATCT CCTAACAGGC 
CTAGAACTCT ATCAAAAAGG AAAGTACCAA GAGGCACTTT CCTACCTGGT ATATGCCTAC 
CAGAGCAATG CTGCCCTGCT GATGAAGGGG CCCCGCCGGG GGGTCAAAGA ATCCGTGATT 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTTTTTGAA 
ACAAATGATG ATCACTCCGT AACTGAGGGC ATTAATGTGA TGAATGAACT GATCATCCCC 
TGCATTCACC TTATCATTAA TAATGACATT TCCAAGGATG ATCTGGATGC CATTGAGGTC 
ATGAGAAACC ATTGGTGCTC TTACCTTGGG CAAGATATTG CAGAAAATCT GCAGCTGTGC 
CTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATCGT CTTGAAAGAG 
CCTCCAACTA TTCGACCCAA TTCTCCCTAT GACCTATGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AGGGAGTTTC AACTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346263.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333192.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu70770
Clone ID Related Accession (Same CDS sequence)	NM_001346263.1 , NM_001346263.2
Accession Version	NM_001346263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform l
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346263.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.33802.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAGC TGTTCTATGG TACTTTCCTG ACTGAAGGGG TTCGTGAAGG AAAACCCTTT 
TGTAACAATG AGACCTTCGG CCAGTATCCT CTTCAGGTAA ACGGTTATCG CAACTTAGAC 
GAGTGTTTGG AAGGGGCCAT GGTGGAGGGT GATGTTGAGC TTCTTCCCTC CGATCACTCG 
GTGAAGTATG GACAAGAGCG TTGGTTTACA AAGCTACCTC CAGTGTTGAC CTTTGAACTC 
TCAAGATTTG AGTTTAATCA GTCCCTTGGG CAGCCAGAGA AAATTCACAA TAAGCTGGAA 
TTTCCTCAGA TTATTTATAT GGACAGGTAC ATGTACAGGA GCAAGGAGCT TATTCGAAAT 
AAGAGAGAGT GTATTCGAAA GTTGAAGGAG GAAATAAAAA TTCTGCAGCA AAAATTGGAA 
AGGTATGTGA AATATGGCTC AGGCCCAGCT CGGTTCCCGC TCCCGGACAT GCTGAAATAT 
GTTATTGAAT TTGCTAGTAC AAAACCTGCC TCAGAAAGCT GTCCACCTGA AAGTGACACA 
CATATGACAT TACCACTTTC TTCAGTGCAC TGCTCGGTTT CTGACCAGAC ATCCAAGGAA 
AGTACAAGTA CAGAAAGCTC TTCTCAGGAT GTTGAAAGTA CCTTTTCTTC TCCTGAAGAT 
TCTTTACCCA AGTCTAAACC ACTGACATCT TCTCGGTCTT CCATGGAAAT GCCTTCACAG 
CCAGCTCCAC GAACAGTCAC AGATGAGGAG ATAAATTTTG TTAAGACCTG TCTTCAGAGA 
TGGAGGAGTG AGATTGAACA AGATATACAA GATTTAAAGA CTTGTATTGC AAGTACTACT 
CAGACTATTG AACAGATGTA CTGCGATCCT CTCCTTCGTC AGGTGCCTTA TCGCTTGCAT 
GCAGTTCTTG TTCATGAAGG ACAAGCAAAT GCTGGACACT ATTGGGCCTA TATCTATAAT 
CAACCCCGAC AGAGCTGGCT CAAGTACAAT GACATCTCTG TTACTGAATC TTCCTGGGAA 
GAAGTTGAAA GAGATTCCTA TGGAGGCCTG AGAAATGTTA GTGCTTACTG TCTGATGTAC 
ATTAATGACA AACTACCCTA CTTCAATGCA GAGGCAGCCC CAACTGAATC AGATCAAATG 
TCAGAAGTGG AAGCCCTATC TGTGGAACTC AAGCATTACA TTCAGGAGGA TAACTGGCGG 
TTTGAGCAGG AAGTAGAGGA GTGGGAAGAA GAGCAGTCTT GCAAAATCCC TCAAATGGAG 
TCCTCCACCA ACTCCTCATC ACAGGACTAC TCTACATCAC AAGAGCCTTC AGTAGCCTCT 
TCTCATGGGG TTCGCTGCTT GTCGTCTGAG CATGCTGTGA TTGTAAAGGA GCAAACTGCC 
CAGGCTATTG CAAACACAGC CCGTGCCTAT GAGAAGAGCG GTGTAGAAGC GGCACTGAGT 
GAGCTTAAAG AAGCTGAACC CAAGAAGCCC ATGCCCCAGG AAACAAACCT TGCAGAGCAG 
TCAGAACAGC CCCCAAAGGC TAATGATGCA GAGTCTACTG CCCAGCCTAA TTCTGAGGTC 
TCTGAAGTCG AGATTCCCAG TGTGGGAAGG ATTCTGGTTA GATCTGATGC AGATGGATAT 
GATGAGGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTGA TTAAGGCATT CCATGAAGAA 
TACTCCAGGC TCTATCAGCT TGCCAAAGAG ACCCCCACCT CTCACAGTGA TCCTCGACTT 
CAGCATGTCC TTGTCTACTT TTTCCAAAAT GAAGCACCCA AAAGGGTAGT AGAACGAACC 
CTTCTGGAAC AGTTTGCAGA TAAAAATCTT AGCTATGATG AAAGATCAAT CAGCATTATG 
AAGGTGGCTC AAGCGAAACT GAAGGAAATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA TAGTTTGTTC CGAAAAGTGT CTGTGTATCT CCTAACAGGC 
CTAGAACTCT ATCAAAAAGG AAAGTACCAA GAGGCACTTT CCTACCTGGT ATATGCCTAC 
CAGAGCAATG CTGCCCTGCT GATGAAGGGG CCCCGCCGGG GGGTCAAAGA ATCCGTGATT 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTTTTTGAA 
ACAAATGATG ATCACTCCGT AACTGAGGGC ATTAATGTGA TGAATGAACT GATCATCCCC 
TGCATTCACC TTATCATTAA TAATGACATT TCCAAGGATG ATCTGGATGC CATTGAGGTC 
ATGAGAAACC ATTGGTGCTC TTACCTTGGG CAAGATATTG CAGAAAATCT GCAGCTGTGC 
CTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATCGT CTTGAAAGAG 
CCTCCAACTA TTCGACCCAA TTCTCCCTAT GACCTATGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AGGGAGTTTC AACTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333192.1
CDS	859..3417
Translation	MVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346263.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346263.2

ORF Insert Sequence:

ATGGTGCAGC TGTTCTATGG TACTTTCCTG ACTGAAGGGG TTCGTGAAGG AAAACCCTTT 
TGTAACAATG AGACCTTCGG CCAGTATCCT CTTCAGGTAA ACGGTTATCG CAACTTAGAC 
GAGTGTTTGG AAGGGGCCAT GGTGGAGGGT GATGTTGAGC TTCTTCCCTC CGATCACTCG 
GTGAAGTATG GACAAGAGCG TTGGTTTACA AAGCTACCTC CAGTGTTGAC CTTTGAACTC 
TCAAGATTTG AGTTTAATCA GTCCCTTGGG CAGCCAGAGA AAATTCACAA TAAGCTGGAA 
TTTCCTCAGA TTATTTATAT GGACAGGTAC ATGTACAGGA GCAAGGAGCT TATTCGAAAT 
AAGAGAGAGT GTATTCGAAA GTTGAAGGAG GAAATAAAAA TTCTGCAGCA AAAATTGGAA 
AGGTATGTGA AATATGGCTC AGGCCCAGCT CGGTTCCCGC TCCCGGACAT GCTGAAATAT 
GTTATTGAAT TTGCTAGTAC AAAACCTGCC TCAGAAAGCT GTCCACCTGA AAGTGACACA 
CATATGACAT TACCACTTTC TTCAGTGCAC TGCTCGGTTT CTGACCAGAC ATCCAAGGAA 
AGTACAAGTA CAGAAAGCTC TTCTCAGGAT GTTGAAAGTA CCTTTTCTTC TCCTGAAGAT 
TCTTTACCCA AGTCTAAACC ACTGACATCT TCTCGGTCTT CCATGGAAAT GCCTTCACAG 
CCAGCTCCAC GAACAGTCAC AGATGAGGAG ATAAATTTTG TTAAGACCTG TCTTCAGAGA 
TGGAGGAGTG AGATTGAACA AGATATACAA GATTTAAAGA CTTGTATTGC AAGTACTACT 
CAGACTATTG AACAGATGTA CTGCGATCCT CTCCTTCGTC AGGTGCCTTA TCGCTTGCAT 
GCAGTTCTTG TTCATGAAGG ACAAGCAAAT GCTGGACACT ATTGGGCCTA TATCTATAAT 
CAACCCCGAC AGAGCTGGCT CAAGTACAAT GACATCTCTG TTACTGAATC TTCCTGGGAA 
GAAGTTGAAA GAGATTCCTA TGGAGGCCTG AGAAATGTTA GTGCTTACTG TCTGATGTAC 
ATTAATGACA AACTACCCTA CTTCAATGCA GAGGCAGCCC CAACTGAATC AGATCAAATG 
TCAGAAGTGG AAGCCCTATC TGTGGAACTC AAGCATTACA TTCAGGAGGA TAACTGGCGG 
TTTGAGCAGG AAGTAGAGGA GTGGGAAGAA GAGCAGTCTT GCAAAATCCC TCAAATGGAG 
TCCTCCACCA ACTCCTCATC ACAGGACTAC TCTACATCAC AAGAGCCTTC AGTAGCCTCT 
TCTCATGGGG TTCGCTGCTT GTCGTCTGAG CATGCTGTGA TTGTAAAGGA GCAAACTGCC 
CAGGCTATTG CAAACACAGC CCGTGCCTAT GAGAAGAGCG GTGTAGAAGC GGCACTGAGT 
GAGCTTAAAG AAGCTGAACC CAAGAAGCCC ATGCCCCAGG AAACAAACCT TGCAGAGCAG 
TCAGAACAGC CCCCAAAGGC TAATGATGCA GAGTCTACTG CCCAGCCTAA TTCTGAGGTC 
TCTGAAGTCG AGATTCCCAG TGTGGGAAGG ATTCTGGTTA GATCTGATGC AGATGGATAT 
GATGAGGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTGA TTAAGGCATT CCATGAAGAA 
TACTCCAGGC TCTATCAGCT TGCCAAAGAG ACCCCCACCT CTCACAGTGA TCCTCGACTT 
CAGCATGTCC TTGTCTACTT TTTCCAAAAT GAAGCACCCA AAAGGGTAGT AGAACGAACC 
CTTCTGGAAC AGTTTGCAGA TAAAAATCTT AGCTATGATG AAAGATCAAT CAGCATTATG 
AAGGTGGCTC AAGCGAAACT GAAGGAAATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA TAGTTTGTTC CGAAAAGTGT CTGTGTATCT CCTAACAGGC 
CTAGAACTCT ATCAAAAAGG AAAGTACCAA GAGGCACTTT CCTACCTGGT ATATGCCTAC 
CAGAGCAATG CTGCCCTGCT GATGAAGGGG CCCCGCCGGG GGGTCAAAGA ATCCGTGATT 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTTTTTGAA 
ACAAATGATG ATCACTCCGT AACTGAGGGC ATTAATGTGA TGAATGAACT GATCATCCCC 
TGCATTCACC TTATCATTAA TAATGACATT TCCAAGGATG ATCTGGATGC CATTGAGGTC 
ATGAGAAACC ATTGGTGCTC TTACCTTGGG CAAGATATTG CAGAAAATCT GCAGCTGTGC 
CTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATCGT CTTGAAAGAG 
CCTCCAACTA TTCGACCCAA TTCTCCCTAT GACCTATGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AGGGAGTTTC AACTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346265.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333194.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 15, mRNA.

Price & Availability↑

CloneID	OHu70771
Clone ID Related Accession (Same CDS sequence)	NM_001346265.2 , NM_001346265.1
Accession Version	NM_001346265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform n
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346265.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.61885.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGCT TAAAGAAGCT 
GAACCCAAGA AGCCCATGCC CCAGGAAACA AACCTTGCAG AGCAGTCAGA ACAGCCCCCA 
AAGGCTAATG ATGCAGAGTC TACTGCCCAG CCTAATTCTG AGGTCTCTGA AGTCGAGATT 
CCCAGTGTGG GAAGGATTCT GGTTAGATCT GATGCAGATG GATATGATGA GGAGGTGATG 
CTGAGCCCTG CCATGCAAGG GGTCATCCTG GCCATAGCTA AAGCCCGTCA GACCTTTGAC 
CGAGATGGGT CTGAAGCAGG GCTGATTAAG GCATTCCATG AAGAATACTC CAGGCTCTAT 
CAGCTTGCCA AAGAGACCCC CACCTCTCAC AGTGATCCTC GACTTCAGCA TGTCCTTGTC 
TACTTTTTCC AAAATGAAGC ACCCAAAAGG GTAGTAGAAC GAACCCTTCT GGAACAGTTT 
GCAGATAAAA ATCTTAGCTA TGATGAAAGA TCAATCAGCA TTATGAAGGT GGCTCAAGCG 
AAACTGAAGG AAATTGGTCC AGATGACATG AATATGGAAG AGTACAAGAA GTGGCATGAA 
GATTATAGTT TGTTCCGAAA AGTGTCTGTG TATCTCCTAA CAGGCCTAGA ACTCTATCAA 
AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC CTGGTATATG CCTACCAGAG CAATGCTGCC 
CTGCTGATGA AGGGGCCCCG CCGGGGGGTC AAAGAATCCG TGATTGCTTT ATACCGAAGA 
AAATGCCTTC TGGAGCTGAA TGCCAAAGCA GCTTCTCTTT TTGAAACAAA TGATGATCAC 
TCCGTAACTG AGGGCATTAA TGTGATGAAT GAACTGATCA TCCCCTGCAT TCACCTTATC 
ATTAATAATG ACATTTCCAA GGATGATCTG GATGCCATTG AGGTCATGAG AAACCATTGG 
TGCTCTTACC TTGGGCAAGA TATTGCAGAA AATCTGCAGC TGTGCCTAGG GGAGTTTCTA 
CCCAGACTTC TAGATCCTTC TGCAGAAATC ATCGTCTTGA AAGAGCCTCC AACTATTCGA 
CCCAATTCTC CCTATGACCT ATGTAGCCGA TTTGCAGCTG TCATGGAGTC AATTCAGGGA 
GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333194.1
CDS	1205..3568
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346265.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346265.2

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGCT TAAAGAAGCT 
GAACCCAAGA AGCCCATGCC CCAGGAAACA AACCTTGCAG AGCAGTCAGA ACAGCCCCCA 
AAGGCTAATG ATGCAGAGTC TACTGCCCAG CCTAATTCTG AGGTCTCTGA AGTCGAGATT 
CCCAGTGTGG GAAGGATTCT GGTTAGATCT GATGCAGATG GATATGATGA GGAGGTGATG 
CTGAGCCCTG CCATGCAAGG GGTCATCCTG GCCATAGCTA AAGCCCGTCA GACCTTTGAC 
CGAGATGGGT CTGAAGCAGG GCTGATTAAG GCATTCCATG AAGAATACTC CAGGCTCTAT 
CAGCTTGCCA AAGAGACCCC CACCTCTCAC AGTGATCCTC GACTTCAGCA TGTCCTTGTC 
TACTTTTTCC AAAATGAAGC ACCCAAAAGG GTAGTAGAAC GAACCCTTCT GGAACAGTTT 
GCAGATAAAA ATCTTAGCTA TGATGAAAGA TCAATCAGCA TTATGAAGGT GGCTCAAGCG 
AAACTGAAGG AAATTGGTCC AGATGACATG AATATGGAAG AGTACAAGAA GTGGCATGAA 
GATTATAGTT TGTTCCGAAA AGTGTCTGTG TATCTCCTAA CAGGCCTAGA ACTCTATCAA 
AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC CTGGTATATG CCTACCAGAG CAATGCTGCC 
CTGCTGATGA AGGGGCCCCG CCGGGGGGTC AAAGAATCCG TGATTGCTTT ATACCGAAGA 
AAATGCCTTC TGGAGCTGAA TGCCAAAGCA GCTTCTCTTT TTGAAACAAA TGATGATCAC 
TCCGTAACTG AGGGCATTAA TGTGATGAAT GAACTGATCA TCCCCTGCAT TCACCTTATC 
ATTAATAATG ACATTTCCAA GGATGATCTG GATGCCATTG AGGTCATGAG AAACCATTGG 
TGCTCTTACC TTGGGCAAGA TATTGCAGAA AATCTGCAGC TGTGCCTAGG GGAGTTTCTA 
CCCAGACTTC TAGATCCTTC TGCAGAAATC ATCGTCTTGA AAGAGCCTCC AACTATTCGA 
CCCAATTCTC CCTATGACCT ATGTAGCCGA TTTGCAGCTG TCATGGAGTC AATTCAGGGA 
GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346265.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333194.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 15, mRNA.

Price & Availability↑

CloneID	OHu70771
Clone ID Related Accession (Same CDS sequence)	NM_001346265.2 , NM_001346265.1
Accession Version	NM_001346265.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform n
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_011542941.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.61885.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGCT TAAAGAAGCT 
GAACCCAAGA AGCCCATGCC CCAGGAAACA AACCTTGCAG AGCAGTCAGA ACAGCCCCCA 
AAGGCTAATG ATGCAGAGTC TACTGCCCAG CCTAATTCTG AGGTCTCTGA AGTCGAGATT 
CCCAGTGTGG GAAGGATTCT GGTTAGATCT GATGCAGATG GATATGATGA GGAGGTGATG 
CTGAGCCCTG CCATGCAAGG GGTCATCCTG GCCATAGCTA AAGCCCGTCA GACCTTTGAC 
CGAGATGGGT CTGAAGCAGG GCTGATTAAG GCATTCCATG AAGAATACTC CAGGCTCTAT 
CAGCTTGCCA AAGAGACCCC CACCTCTCAC AGTGATCCTC GACTTCAGCA TGTCCTTGTC 
TACTTTTTCC AAAATGAAGC ACCCAAAAGG GTAGTAGAAC GAACCCTTCT GGAACAGTTT 
GCAGATAAAA ATCTTAGCTA TGATGAAAGA TCAATCAGCA TTATGAAGGT GGCTCAAGCG 
AAACTGAAGG AAATTGGTCC AGATGACATG AATATGGAAG AGTACAAGAA GTGGCATGAA 
GATTATAGTT TGTTCCGAAA AGTGTCTGTG TATCTCCTAA CAGGCCTAGA ACTCTATCAA 
AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC CTGGTATATG CCTACCAGAG CAATGCTGCC 
CTGCTGATGA AGGGGCCCCG CCGGGGGGTC AAAGAATCCG TGATTGCTTT ATACCGAAGA 
AAATGCCTTC TGGAGCTGAA TGCCAAAGCA GCTTCTCTTT TTGAAACAAA TGATGATCAC 
TCCGTAACTG AGGGCATTAA TGTGATGAAT GAACTGATCA TCCCCTGCAT TCACCTTATC 
ATTAATAATG ACATTTCCAA GGATGATCTG GATGCCATTG AGGTCATGAG AAACCATTGG 
TGCTCTTACC TTGGGCAAGA TATTGCAGAA AATCTGCAGC TGTGCCTAGG GGAGTTTCTA 
CCCAGACTTC TAGATCCTTC TGCAGAAATC ATCGTCTTGA AAGAGCCTCC AACTATTCGA 
CCCAATTCTC CCTATGACCT ATGTAGCCGA TTTGCAGCTG TCATGGAGTC AATTCAGGGA 
GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333194.1
CDS	1203..3566
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346265.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346265.1

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGCT TAAAGAAGCT 
GAACCCAAGA AGCCCATGCC CCAGGAAACA AACCTTGCAG AGCAGTCAGA ACAGCCCCCA 
AAGGCTAATG ATGCAGAGTC TACTGCCCAG CCTAATTCTG AGGTCTCTGA AGTCGAGATT 
CCCAGTGTGG GAAGGATTCT GGTTAGATCT GATGCAGATG GATATGATGA GGAGGTGATG 
CTGAGCCCTG CCATGCAAGG GGTCATCCTG GCCATAGCTA AAGCCCGTCA GACCTTTGAC 
CGAGATGGGT CTGAAGCAGG GCTGATTAAG GCATTCCATG AAGAATACTC CAGGCTCTAT 
CAGCTTGCCA AAGAGACCCC CACCTCTCAC AGTGATCCTC GACTTCAGCA TGTCCTTGTC 
TACTTTTTCC AAAATGAAGC ACCCAAAAGG GTAGTAGAAC GAACCCTTCT GGAACAGTTT 
GCAGATAAAA ATCTTAGCTA TGATGAAAGA TCAATCAGCA TTATGAAGGT GGCTCAAGCG 
AAACTGAAGG AAATTGGTCC AGATGACATG AATATGGAAG AGTACAAGAA GTGGCATGAA 
GATTATAGTT TGTTCCGAAA AGTGTCTGTG TATCTCCTAA CAGGCCTAGA ACTCTATCAA 
AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC CTGGTATATG CCTACCAGAG CAATGCTGCC 
CTGCTGATGA AGGGGCCCCG CCGGGGGGTC AAAGAATCCG TGATTGCTTT ATACCGAAGA 
AAATGCCTTC TGGAGCTGAA TGCCAAAGCA GCTTCTCTTT TTGAAACAAA TGATGATCAC 
TCCGTAACTG AGGGCATTAA TGTGATGAAT GAACTGATCA TCCCCTGCAT TCACCTTATC 
ATTAATAATG ACATTTCCAA GGATGATCTG GATGCCATTG AGGTCATGAG AAACCATTGG 
TGCTCTTACC TTGGGCAAGA TATTGCAGAA AATCTGCAGC TGTGCCTAGG GGAGTTTCTA 
CCCAGACTTC TAGATCCTTC TGCAGAAATC ATCGTCTTGA AAGAGCCTCC AACTATTCGA 
CCCAATTCTC CCTATGACCT ATGTAGCCGA TTTGCAGCTG TCATGGAGTC AATTCAGGGA 
GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_011542942.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541244.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X24, mRNA.

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CloneID	OHu70772
Clone ID Related Accession (Same CDS sequence)	XM_011542942.1
Accession Version	XM_011542942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT ATGTTATTGA ATTTGCTAGT ACAAAACCTG CCTCAGAAAG CTGTCCACCT 
GAAAGTGACA CACATATGAC ATTACCACTT TCTTCAGTGC ACTGCTCGGT TTCTGACCAG 
ACATCCAAGG AAAGTACAAG TACAGAAAGC TCTTCTCAGG ATGTTGAAAG TACCTTTTCT 
TCTCCTGAAG ATTCTTTACC CAAGTCTAAA CCACTGACAT CTTCTCGGTC TTCCATGGAA 
ATGCCTTCAC AGCCAGCTCC ACGAACAGTC ACAGATGAGG AGATAAATTT TGTTAAGACC 
TGTCTTCAGA GATGGAGGAG TGAGATTGAA CAAGATATAC AAGATTTAAA GACTTGTATT 
GCAAGTACTA CTCAGACTAT TGAACAGATG TACTGCGATC CTCTCCTTCG TCAGGTGCCT 
TATCGCTTGC ATGCAGTTCT TGTTCATGAA GGACAAGCAA ATGCTGGACA CTATTGGGCC 
TATATCTATA ATCAACCCCG ACAGAGCTGG CTCAAGTACA ATGACATCTC TGTTACTGAA 
TCTTCCTGGG AAGAAGTTGA AAGAGATTCC TATGGAGGCC TGAGAAATGT TAGTGCTTAC 
TGTCTGATGT ACATTAATGA CAAACTACCC TACTTCAATG CAGAGGCAGC CCCAACTGAA 
TCAGATCAAA TGTCAGAAGT GGAAGCCCTA TCTGTGGAAC TCAAGCATTA CATTCAGGAG 
GATAACTGGC GGTTTGAGCA GGAAGTAGAG GAGTGGGAAG AAGAGCAGTC TTGCAAAATC 
CCTCAAATGG AGTCCTCCAC CAACTCCTCA TCACAGGACT ACTCTACATC ACAAGAGCCT 
TCAGTAGCCT CTTCTCATGG GGTTCGCTGC TTGTCGTCTG AGCATGCTGT GATTGTAAAG 
GAGCAAACTG CCCAGGCTAT TGCAAACACA GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA 
GCGGCACTGA GTGAGCTTAA AGAAGCTGAA CCCAAGAAGC CCATGCCCCA GGAAACAAAC 
CTTGCAGAGC AGTCAGAACA GCCCCCAAAG GCTAATGATG CAGAGTCTAC TGCCCAGCCT 
AATTCTGAGG TCTCTGAAGT CGAGATTCCC AGTGTGGGAA GGATTCTGGT TAGATCTGAT 
GCAGATGGAT ATGATGAGGA GGTGATGCTG AGCCCTGCCA TGCAAGGGGT CATCCTGGCC 
ATAGCTAAAG CCCGTCAGAC CTTTGACCGA GATGGGTCTG AAGCAGGGCT GATTAAGGCA 
TTCCATGAAG AATACTCCAG GCTCTATCAG CTTGCCAAAG AGACCCCCAC CTCTCACAGT 
GATCCTCGAC TTCAGCATGT CCTTGTCTAC TTTTTCCAAA ATGAAGCACC CAAAAGGGTA 
GTAGAACGAA CCCTTCTGGA ACAGTTTGCA GATAAAAATC TTAGCTATGA TGAAAGATCA 
ATCAGCATTA TGAAGGTGGC TCAAGCGAAA CTGAAGGAAA TTGGTCCAGA TGACATGAAT 
ATGGAAGAGT ACAAGAAGTG GCATGAAGAT TATAGTTTGT TCCGAAAAGT GTCTGTGTAT 
CTCCTAACAG GCCTAGAACT CTATCAAAAA GGAAAGTACC AAGAGGCACT TTCCTACCTG 
GTATATGCCT ACCAGAGCAA TGCTGCCCTG CTGATGAAGG GGCCCCGCCG GGGGGTCAAA 
GAATCCGTGA TTGCTTTATA CCGAAGAAAA TGCCTTCTGG AGCTGAATGC CAAAGCAGCT 
TCTCTTTTTG AAACAAATGA TGATCACTCC GTAACTGAGG GCATTAATGT GATGAATGAA 
CTGATCATCC CCTGCATTCA CCTTATCATT AATAATGACA TTTCCAAGGA TGATCTGGAT 
GCCATTGAGG TCATGAGAAA CCATTGGTGC TCTTACCTTG GGCAAGATAT TGCAGAAAAT 
CTGCAGCTGT GCCTAGGGGA GTTTCTACCC AGACTTCTAG ATCCTTCTGC AGAAATCATC 
GTCTTGAAAG AGCCTCCAAC TATTCGACCC AATTCTCCCT ATGACCTATG TAGCCGATTT 
GCAGCTGTCA TGGAGTCAAT TCAGGGAGTT TCAACTGTGA CAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541244.1
CDS	114..2204
Translation	MLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_011542942.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542942.1

ORF Insert Sequence:

ATGCTGAAAT ATGTTATTGA ATTTGCTAGT ACAAAACCTG CCTCAGAAAG CTGTCCACCT 
GAAAGTGACA CACATATGAC ATTACCACTT TCTTCAGTGC ACTGCTCGGT TTCTGACCAG 
ACATCCAAGG AAAGTACAAG TACAGAAAGC TCTTCTCAGG ATGTTGAAAG TACCTTTTCT 
TCTCCTGAAG ATTCTTTACC CAAGTCTAAA CCACTGACAT CTTCTCGGTC TTCCATGGAA 
ATGCCTTCAC AGCCAGCTCC ACGAACAGTC ACAGATGAGG AGATAAATTT TGTTAAGACC 
TGTCTTCAGA GATGGAGGAG TGAGATTGAA CAAGATATAC AAGATTTAAA GACTTGTATT 
GCAAGTACTA CTCAGACTAT TGAACAGATG TACTGCGATC CTCTCCTTCG TCAGGTGCCT 
TATCGCTTGC ATGCAGTTCT TGTTCATGAA GGACAAGCAA ATGCTGGACA CTATTGGGCC 
TATATCTATA ATCAACCCCG ACAGAGCTGG CTCAAGTACA ATGACATCTC TGTTACTGAA 
TCTTCCTGGG AAGAAGTTGA AAGAGATTCC TATGGAGGCC TGAGAAATGT TAGTGCTTAC 
TGTCTGATGT ACATTAATGA CAAACTACCC TACTTCAATG CAGAGGCAGC CCCAACTGAA 
TCAGATCAAA TGTCAGAAGT GGAAGCCCTA TCTGTGGAAC TCAAGCATTA CATTCAGGAG 
GATAACTGGC GGTTTGAGCA GGAAGTAGAG GAGTGGGAAG AAGAGCAGTC TTGCAAAATC 
CCTCAAATGG AGTCCTCCAC CAACTCCTCA TCACAGGACT ACTCTACATC ACAAGAGCCT 
TCAGTAGCCT CTTCTCATGG GGTTCGCTGC TTGTCGTCTG AGCATGCTGT GATTGTAAAG 
GAGCAAACTG CCCAGGCTAT TGCAAACACA GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA 
GCGGCACTGA GTGAGCTTAA AGAAGCTGAA CCCAAGAAGC CCATGCCCCA GGAAACAAAC 
CTTGCAGAGC AGTCAGAACA GCCCCCAAAG GCTAATGATG CAGAGTCTAC TGCCCAGCCT 
AATTCTGAGG TCTCTGAAGT CGAGATTCCC AGTGTGGGAA GGATTCTGGT TAGATCTGAT 
GCAGATGGAT ATGATGAGGA GGTGATGCTG AGCCCTGCCA TGCAAGGGGT CATCCTGGCC 
ATAGCTAAAG CCCGTCAGAC CTTTGACCGA GATGGGTCTG AAGCAGGGCT GATTAAGGCA 
TTCCATGAAG AATACTCCAG GCTCTATCAG CTTGCCAAAG AGACCCCCAC CTCTCACAGT 
GATCCTCGAC TTCAGCATGT CCTTGTCTAC TTTTTCCAAA ATGAAGCACC CAAAAGGGTA 
GTAGAACGAA CCCTTCTGGA ACAGTTTGCA GATAAAAATC TTAGCTATGA TGAAAGATCA 
ATCAGCATTA TGAAGGTGGC TCAAGCGAAA CTGAAGGAAA TTGGTCCAGA TGACATGAAT 
ATGGAAGAGT ACAAGAAGTG GCATGAAGAT TATAGTTTGT TCCGAAAAGT GTCTGTGTAT 
CTCCTAACAG GCCTAGAACT CTATCAAAAA GGAAAGTACC AAGAGGCACT TTCCTACCTG 
GTATATGCCT ACCAGAGCAA TGCTGCCCTG CTGATGAAGG GGCCCCGCCG GGGGGTCAAA 
GAATCCGTGA TTGCTTTATA CCGAAGAAAA TGCCTTCTGG AGCTGAATGC CAAAGCAGCT 
TCTCTTTTTG AAACAAATGA TGATCACTCC GTAACTGAGG GCATTAATGT GATGAATGAA 
CTGATCATCC CCTGCATTCA CCTTATCATT AATAATGACA TTTCCAAGGA TGATCTGGAT 
GCCATTGAGG TCATGAGAAA CCATTGGTGC TCTTACCTTG GGCAAGATAT TGCAGAAAAT 
CTGCAGCTGT GCCTAGGGGA GTTTCTACCC AGACTTCTAG ATCCTTCTGC AGAAATCATC 
GTCTTGAAAG AGCCTCCAAC TATTCGACCC AATTCTCCCT ATGACCTATG TAGCCGATTT 
GCAGCTGTCA TGGAGTCAAT TCAGGGAGTT TCAACTGTGA CAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346261.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333190.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 11, mRNA.

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CloneID	OHu100563
Clone ID Related Accession (Same CDS sequence)	NM_001346261.2 , NM_001346261.1
Accession Version	NM_001346261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform k
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346261.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.78441.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTGA TTAAGGCATT CCATGAAGAA 
TACTCCAGGC TCTATCAGCT TGCCAAAGAG ACCCCCACCT CTCACAGTGA TCCTCGACTT 
CAGCATGTCC TTGTCTACTT TTTCCAAAAT GAAGCACCCA AAAGGGTAGT AGAACGAACC 
CTTCTGGAAC AGTTTGCAGA TAAAAATCTT AGCTATGATG AAAGATCAAT CAGCATTATG 
AAGGTGGCTC AAGCGAAACT GAAGGAAATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA TAGTTTGTTC CGAAAAGTGT CTGTGTATCT CCTAACAGGC 
CTAGAACTCT ATCAAAAAGG AAAGTACCAA GAGGCACTTT CCTACCTGGT ATATGCCTAC 
CAGAGCAATG CTGCCCTGCT GATGAAGGGG CCCCGCCGGG GGGTCAAAGA ATCCGTGATT 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTTTTTGAA 
ACAAATGATG ATCACTCCGT AACTGAGGGC ATTAATGTGA TGAATGAACT GATCATCCCC 
TGCATTCACC TTATCATTAA TAATGACATT TCCAAGGATG ATCTGGATGC CATTGAGGTC 
ATGAGAAACC ATTGGTGCTC TTACCTTGGG CAAGATATTG CAGAAAATCT GCAGCTGTGC 
CTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATCGT CTTGAAAGAG 
CCTCCAACTA TTCGACCCAA TTCTCCCTAT GACCTATGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AGGGAGTTTC AACTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333190.1
CDS	236..3094
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346261.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346261.2

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTGA TTAAGGCATT CCATGAAGAA 
TACTCCAGGC TCTATCAGCT TGCCAAAGAG ACCCCCACCT CTCACAGTGA TCCTCGACTT 
CAGCATGTCC TTGTCTACTT TTTCCAAAAT GAAGCACCCA AAAGGGTAGT AGAACGAACC 
CTTCTGGAAC AGTTTGCAGA TAAAAATCTT AGCTATGATG AAAGATCAAT CAGCATTATG 
AAGGTGGCTC AAGCGAAACT GAAGGAAATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA TAGTTTGTTC CGAAAAGTGT CTGTGTATCT CCTAACAGGC 
CTAGAACTCT ATCAAAAAGG AAAGTACCAA GAGGCACTTT CCTACCTGGT ATATGCCTAC 
CAGAGCAATG CTGCCCTGCT GATGAAGGGG CCCCGCCGGG GGGTCAAAGA ATCCGTGATT 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTTTTTGAA 
ACAAATGATG ATCACTCCGT AACTGAGGGC ATTAATGTGA TGAATGAACT GATCATCCCC 
TGCATTCACC TTATCATTAA TAATGACATT TCCAAGGATG ATCTGGATGC CATTGAGGTC 
ATGAGAAACC ATTGGTGCTC TTACCTTGGG CAAGATATTG CAGAAAATCT GCAGCTGTGC 
CTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATCGT CTTGAAAGAG 
CCTCCAACTA TTCGACCCAA TTCTCCCTAT GACCTATGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AGGGAGTTTC AACTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346261.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333190.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu100563
Clone ID Related Accession (Same CDS sequence)	NM_001346261.2 , NM_001346261.1
Accession Version	NM_001346261.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform k
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018062.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.78441.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTGA TTAAGGCATT CCATGAAGAA 
TACTCCAGGC TCTATCAGCT TGCCAAAGAG ACCCCCACCT CTCACAGTGA TCCTCGACTT 
CAGCATGTCC TTGTCTACTT TTTCCAAAAT GAAGCACCCA AAAGGGTAGT AGAACGAACC 
CTTCTGGAAC AGTTTGCAGA TAAAAATCTT AGCTATGATG AAAGATCAAT CAGCATTATG 
AAGGTGGCTC AAGCGAAACT GAAGGAAATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA TAGTTTGTTC CGAAAAGTGT CTGTGTATCT CCTAACAGGC 
CTAGAACTCT ATCAAAAAGG AAAGTACCAA GAGGCACTTT CCTACCTGGT ATATGCCTAC 
CAGAGCAATG CTGCCCTGCT GATGAAGGGG CCCCGCCGGG GGGTCAAAGA ATCCGTGATT 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTTTTTGAA 
ACAAATGATG ATCACTCCGT AACTGAGGGC ATTAATGTGA TGAATGAACT GATCATCCCC 
TGCATTCACC TTATCATTAA TAATGACATT TCCAAGGATG ATCTGGATGC CATTGAGGTC 
ATGAGAAACC ATTGGTGCTC TTACCTTGGG CAAGATATTG CAGAAAATCT GCAGCTGTGC 
CTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATCGT CTTGAAAGAG 
CCTCCAACTA TTCGACCCAA TTCTCCCTAT GACCTATGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AGGGAGTTTC AACTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333190.1
CDS	234..3092
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346261.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346261.1

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGG TGATGCTGAG CCCTGCCATG CAAGGGGTCA TCCTGGCCAT AGCTAAAGCC 
CGTCAGACCT TTGACCGAGA TGGGTCTGAA GCAGGGCTGA TTAAGGCATT CCATGAAGAA 
TACTCCAGGC TCTATCAGCT TGCCAAAGAG ACCCCCACCT CTCACAGTGA TCCTCGACTT 
CAGCATGTCC TTGTCTACTT TTTCCAAAAT GAAGCACCCA AAAGGGTAGT AGAACGAACC 
CTTCTGGAAC AGTTTGCAGA TAAAAATCTT AGCTATGATG AAAGATCAAT CAGCATTATG 
AAGGTGGCTC AAGCGAAACT GAAGGAAATT GGTCCAGATG ACATGAATAT GGAAGAGTAC 
AAGAAGTGGC ATGAAGATTA TAGTTTGTTC CGAAAAGTGT CTGTGTATCT CCTAACAGGC 
CTAGAACTCT ATCAAAAAGG AAAGTACCAA GAGGCACTTT CCTACCTGGT ATATGCCTAC 
CAGAGCAATG CTGCCCTGCT GATGAAGGGG CCCCGCCGGG GGGTCAAAGA ATCCGTGATT 
GCTTTATACC GAAGAAAATG CCTTCTGGAG CTGAATGCCA AAGCAGCTTC TCTTTTTGAA 
ACAAATGATG ATCACTCCGT AACTGAGGGC ATTAATGTGA TGAATGAACT GATCATCCCC 
TGCATTCACC TTATCATTAA TAATGACATT TCCAAGGATG ATCTGGATGC CATTGAGGTC 
ATGAGAAACC ATTGGTGCTC TTACCTTGGG CAAGATATTG CAGAAAATCT GCAGCTGTGC 
CTAGGGGAGT TTCTACCCAG ACTTCTAGAT CCTTCTGCAG AAATCATCGT CTTGAAAGAG 
CCTCCAACTA TTCGACCCAA TTCTCCCTAT GACCTATGTA GCCGATTTGC AGCTGTCATG 
GAGTCAATTC AGGGAGTTTC AACTGTGACA GTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346272.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333201.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 21, mRNA.

Price & Availability↑

CloneID	OHu100589
Clone ID Related Accession (Same CDS sequence)	NM_001346272.2 , NM_001346272.1
Accession Version	NM_001346272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform q
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346272.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.20203.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333201.1
CDS	997..3078
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346272.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346272.2

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346272.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333201.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 21, mRNA.

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CloneID	OHu100589
Clone ID Related Accession (Same CDS sequence)	NM_001346272.2 , NM_001346272.1
Accession Version	NM_001346272.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform q
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018068.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.20203.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333201.1
CDS	995..3076
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346272.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346272.1

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346268.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333197.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 17, mRNA.

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CloneID	OHu100612
Clone ID Related Accession (Same CDS sequence)	NM_001346268.2 , NM_001346267.2 , NM_001346267.1 , NM_001346268.1
Accession Version	NM_001346268.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform o
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346268.1. Transcript Variant: This variant (17), as well as variant 16, encodes isoform o. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.165663.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTGATTAA GGCATTCCAT GAAGAATACT CCAGGCTCTA TCAGCTTGCC 
AAAGAGACCC CCACCTCTCA CAGTGATCCT CGACTTCAGC ATGTCCTTGT CTACTTTTTC 
CAAAATGAAG CACCCAAAAG GGTAGTAGAA CGAACCCTTC TGGAACAGTT TGCAGATAAA 
AATCTTAGCT ATGATGAAAG ATCAATCAGC ATTATGAAGG TGGCTCAAGC GAAACTGAAG 
GAAATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTATAGT 
TTGTTCCGAA AAGTGTCTGT GTATCTCCTA ACAGGCCTAG AACTCTATCA AAAAGGAAAG 
TACCAAGAGG CACTTTCCTA CCTGGTATAT GCCTACCAGA GCAATGCTGC CCTGCTGATG 
AAGGGGCCCC GCCGGGGGGT CAAAGAATCC GTGATTGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT TTTGAAACAA ATGATGATCA CTCCGTAACT 
GAGGGCATTA ATGTGATGAA TGAACTGATC ATCCCCTGCA TTCACCTTAT CATTAATAAT 
GACATTTCCA AGGATGATCT GGATGCCATT GAGGTCATGA GAAACCATTG GTGCTCTTAC 
CTTGGGCAAG ATATTGCAGA AAATCTGCAG CTGTGCCTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATCGTCTTG AAAGAGCCTC CAACTATTCG ACCCAATTCT 
CCCTATGACC TATGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAGGG AGTTTCAACT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333197.1
CDS	709..2943
Translation	MVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346268.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346268.2

ORF Insert Sequence:

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTGATTAA GGCATTCCAT GAAGAATACT CCAGGCTCTA TCAGCTTGCC 
AAAGAGACCC CCACCTCTCA CAGTGATCCT CGACTTCAGC ATGTCCTTGT CTACTTTTTC 
CAAAATGAAG CACCCAAAAG GGTAGTAGAA CGAACCCTTC TGGAACAGTT TGCAGATAAA 
AATCTTAGCT ATGATGAAAG ATCAATCAGC ATTATGAAGG TGGCTCAAGC GAAACTGAAG 
GAAATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTATAGT 
TTGTTCCGAA AAGTGTCTGT GTATCTCCTA ACAGGCCTAG AACTCTATCA AAAAGGAAAG 
TACCAAGAGG CACTTTCCTA CCTGGTATAT GCCTACCAGA GCAATGCTGC CCTGCTGATG 
AAGGGGCCCC GCCGGGGGGT CAAAGAATCC GTGATTGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT TTTGAAACAA ATGATGATCA CTCCGTAACT 
GAGGGCATTA ATGTGATGAA TGAACTGATC ATCCCCTGCA TTCACCTTAT CATTAATAAT 
GACATTTCCA AGGATGATCT GGATGCCATT GAGGTCATGA GAAACCATTG GTGCTCTTAC 
CTTGGGCAAG ATATTGCAGA AAATCTGCAG CTGTGCCTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATCGTCTTG AAAGAGCCTC CAACTATTCG ACCCAATTCT 
CCCTATGACC TATGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAGGG AGTTTCAACT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346267.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333196.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 16, mRNA.

Price & Availability↑

CloneID	OHu100612
Clone ID Related Accession (Same CDS sequence)	NM_001346268.2 , NM_001346267.2 , NM_001346267.1 , NM_001346268.1
Accession Version	NM_001346267.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform o
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346267.1. Transcript Variant: This variant (16), as well as variant 17, encodes isoform o. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.100710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTGATTAA GGCATTCCAT GAAGAATACT CCAGGCTCTA TCAGCTTGCC 
AAAGAGACCC CCACCTCTCA CAGTGATCCT CGACTTCAGC ATGTCCTTGT CTACTTTTTC 
CAAAATGAAG CACCCAAAAG GGTAGTAGAA CGAACCCTTC TGGAACAGTT TGCAGATAAA 
AATCTTAGCT ATGATGAAAG ATCAATCAGC ATTATGAAGG TGGCTCAAGC GAAACTGAAG 
GAAATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTATAGT 
TTGTTCCGAA AAGTGTCTGT GTATCTCCTA ACAGGCCTAG AACTCTATCA AAAAGGAAAG 
TACCAAGAGG CACTTTCCTA CCTGGTATAT GCCTACCAGA GCAATGCTGC CCTGCTGATG 
AAGGGGCCCC GCCGGGGGGT CAAAGAATCC GTGATTGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT TTTGAAACAA ATGATGATCA CTCCGTAACT 
GAGGGCATTA ATGTGATGAA TGAACTGATC ATCCCCTGCA TTCACCTTAT CATTAATAAT 
GACATTTCCA AGGATGATCT GGATGCCATT GAGGTCATGA GAAACCATTG GTGCTCTTAC 
CTTGGGCAAG ATATTGCAGA AAATCTGCAG CTGTGCCTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATCGTCTTG AAAGAGCCTC CAACTATTCG ACCCAATTCT 
CCCTATGACC TATGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAGGG AGTTTCAACT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333196.1
CDS	920..3154
Translation	MVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346267.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346267.2

ORF Insert Sequence:

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTGATTAA GGCATTCCAT GAAGAATACT CCAGGCTCTA TCAGCTTGCC 
AAAGAGACCC CCACCTCTCA CAGTGATCCT CGACTTCAGC ATGTCCTTGT CTACTTTTTC 
CAAAATGAAG CACCCAAAAG GGTAGTAGAA CGAACCCTTC TGGAACAGTT TGCAGATAAA 
AATCTTAGCT ATGATGAAAG ATCAATCAGC ATTATGAAGG TGGCTCAAGC GAAACTGAAG 
GAAATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTATAGT 
TTGTTCCGAA AAGTGTCTGT GTATCTCCTA ACAGGCCTAG AACTCTATCA AAAAGGAAAG 
TACCAAGAGG CACTTTCCTA CCTGGTATAT GCCTACCAGA GCAATGCTGC CCTGCTGATG 
AAGGGGCCCC GCCGGGGGGT CAAAGAATCC GTGATTGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT TTTGAAACAA ATGATGATCA CTCCGTAACT 
GAGGGCATTA ATGTGATGAA TGAACTGATC ATCCCCTGCA TTCACCTTAT CATTAATAAT 
GACATTTCCA AGGATGATCT GGATGCCATT GAGGTCATGA GAAACCATTG GTGCTCTTAC 
CTTGGGCAAG ATATTGCAGA AAATCTGCAG CTGTGCCTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATCGTCTTG AAAGAGCCTC CAACTATTCG ACCCAATTCT 
CCCTATGACC TATGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAGGG AGTTTCAACT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346267.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333196.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 16, mRNA.

Price & Availability↑

CloneID	OHu100612
Clone ID Related Accession (Same CDS sequence)	NM_001346268.2 , NM_001346267.2 , NM_001346267.1 , NM_001346268.1
Accession Version	NM_001346267.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform o
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. Transcript Variant: This variant (16), as well as variant 17, encodes isoform o. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.100710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTGATTAA GGCATTCCAT GAAGAATACT CCAGGCTCTA TCAGCTTGCC 
AAAGAGACCC CCACCTCTCA CAGTGATCCT CGACTTCAGC ATGTCCTTGT CTACTTTTTC 
CAAAATGAAG CACCCAAAAG GGTAGTAGAA CGAACCCTTC TGGAACAGTT TGCAGATAAA 
AATCTTAGCT ATGATGAAAG ATCAATCAGC ATTATGAAGG TGGCTCAAGC GAAACTGAAG 
GAAATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTATAGT 
TTGTTCCGAA AAGTGTCTGT GTATCTCCTA ACAGGCCTAG AACTCTATCA AAAAGGAAAG 
TACCAAGAGG CACTTTCCTA CCTGGTATAT GCCTACCAGA GCAATGCTGC CCTGCTGATG 
AAGGGGCCCC GCCGGGGGGT CAAAGAATCC GTGATTGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT TTTGAAACAA ATGATGATCA CTCCGTAACT 
GAGGGCATTA ATGTGATGAA TGAACTGATC ATCCCCTGCA TTCACCTTAT CATTAATAAT 
GACATTTCCA AGGATGATCT GGATGCCATT GAGGTCATGA GAAACCATTG GTGCTCTTAC 
CTTGGGCAAG ATATTGCAGA AAATCTGCAG CTGTGCCTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATCGTCTTG AAAGAGCCTC CAACTATTCG ACCCAATTCT 
CCCTATGACC TATGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAGGG AGTTTCAACT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333196.1
CDS	918..3152
Translation	MVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346267.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346267.1

ORF Insert Sequence:

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTGATTAA GGCATTCCAT GAAGAATACT CCAGGCTCTA TCAGCTTGCC 
AAAGAGACCC CCACCTCTCA CAGTGATCCT CGACTTCAGC ATGTCCTTGT CTACTTTTTC 
CAAAATGAAG CACCCAAAAG GGTAGTAGAA CGAACCCTTC TGGAACAGTT TGCAGATAAA 
AATCTTAGCT ATGATGAAAG ATCAATCAGC ATTATGAAGG TGGCTCAAGC GAAACTGAAG 
GAAATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTATAGT 
TTGTTCCGAA AAGTGTCTGT GTATCTCCTA ACAGGCCTAG AACTCTATCA AAAAGGAAAG 
TACCAAGAGG CACTTTCCTA CCTGGTATAT GCCTACCAGA GCAATGCTGC CCTGCTGATG 
AAGGGGCCCC GCCGGGGGGT CAAAGAATCC GTGATTGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT TTTGAAACAA ATGATGATCA CTCCGTAACT 
GAGGGCATTA ATGTGATGAA TGAACTGATC ATCCCCTGCA TTCACCTTAT CATTAATAAT 
GACATTTCCA AGGATGATCT GGATGCCATT GAGGTCATGA GAAACCATTG GTGCTCTTAC 
CTTGGGCAAG ATATTGCAGA AAATCTGCAG CTGTGCCTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATCGTCTTG AAAGAGCCTC CAACTATTCG ACCCAATTCT 
CCCTATGACC TATGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAGGG AGTTTCAACT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346268.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333197.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 17, mRNA.

Price & Availability↑

CloneID	OHu100612
Clone ID Related Accession (Same CDS sequence)	NM_001346268.2 , NM_001346267.2 , NM_001346267.1 , NM_001346268.1
Accession Version	NM_001346268.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform o
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. Transcript Variant: This variant (17), as well as variant 16, encodes isoform o. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.165663.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTGATTAA GGCATTCCAT GAAGAATACT CCAGGCTCTA TCAGCTTGCC 
AAAGAGACCC CCACCTCTCA CAGTGATCCT CGACTTCAGC ATGTCCTTGT CTACTTTTTC 
CAAAATGAAG CACCCAAAAG GGTAGTAGAA CGAACCCTTC TGGAACAGTT TGCAGATAAA 
AATCTTAGCT ATGATGAAAG ATCAATCAGC ATTATGAAGG TGGCTCAAGC GAAACTGAAG 
GAAATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTATAGT 
TTGTTCCGAA AAGTGTCTGT GTATCTCCTA ACAGGCCTAG AACTCTATCA AAAAGGAAAG 
TACCAAGAGG CACTTTCCTA CCTGGTATAT GCCTACCAGA GCAATGCTGC CCTGCTGATG 
AAGGGGCCCC GCCGGGGGGT CAAAGAATCC GTGATTGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT TTTGAAACAA ATGATGATCA CTCCGTAACT 
GAGGGCATTA ATGTGATGAA TGAACTGATC ATCCCCTGCA TTCACCTTAT CATTAATAAT 
GACATTTCCA AGGATGATCT GGATGCCATT GAGGTCATGA GAAACCATTG GTGCTCTTAC 
CTTGGGCAAG ATATTGCAGA AAATCTGCAG CTGTGCCTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATCGTCTTG AAAGAGCCTC CAACTATTCG ACCCAATTCT 
CCCTATGACC TATGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAGGG AGTTTCAACT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333197.1
CDS	707..2941
Translation	MVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346268.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346268.1

ORF Insert Sequence:

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGGTGAT GCTGAGCCCT 
GCCATGCAAG GGGTCATCCT GGCCATAGCT AAAGCCCGTC AGACCTTTGA CCGAGATGGG 
TCTGAAGCAG GGCTGATTAA GGCATTCCAT GAAGAATACT CCAGGCTCTA TCAGCTTGCC 
AAAGAGACCC CCACCTCTCA CAGTGATCCT CGACTTCAGC ATGTCCTTGT CTACTTTTTC 
CAAAATGAAG CACCCAAAAG GGTAGTAGAA CGAACCCTTC TGGAACAGTT TGCAGATAAA 
AATCTTAGCT ATGATGAAAG ATCAATCAGC ATTATGAAGG TGGCTCAAGC GAAACTGAAG 
GAAATTGGTC CAGATGACAT GAATATGGAA GAGTACAAGA AGTGGCATGA AGATTATAGT 
TTGTTCCGAA AAGTGTCTGT GTATCTCCTA ACAGGCCTAG AACTCTATCA AAAAGGAAAG 
TACCAAGAGG CACTTTCCTA CCTGGTATAT GCCTACCAGA GCAATGCTGC CCTGCTGATG 
AAGGGGCCCC GCCGGGGGGT CAAAGAATCC GTGATTGCTT TATACCGAAG AAAATGCCTT 
CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT TTTGAAACAA ATGATGATCA CTCCGTAACT 
GAGGGCATTA ATGTGATGAA TGAACTGATC ATCCCCTGCA TTCACCTTAT CATTAATAAT 
GACATTTCCA AGGATGATCT GGATGCCATT GAGGTCATGA GAAACCATTG GTGCTCTTAC 
CTTGGGCAAG ATATTGCAGA AAATCTGCAG CTGTGCCTAG GGGAGTTTCT ACCCAGACTT 
CTAGATCCTT CTGCAGAAAT CATCGTCTTG AAAGAGCCTC CAACTATTCG ACCCAATTCT 
CCCTATGACC TATGTAGCCG ATTTGCAGCT GTCATGGAGT CAATTCAGGG AGTTTCAACT 
GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346264.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333193.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 14, mRNA.

Price & Availability↑

CloneID	OHu100639
Clone ID Related Accession (Same CDS sequence)	NM_001346264.1 , NM_001346264.2
Accession Version	NM_001346264.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform m
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.41923.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGCTTAA AGAAGCTGAA 
CCCAAGAAGC CCATGCCCCA GGAAACAAAC CTTGCAGAGC AGTCAGAACA GCCCCCAAAG 
GCTAATGATG CAGAGTCTAC TGCCCAGCCT AATTCTGAGG TCTCTGAAGT CGAGATTCCC 
AGTGTGGGAA GGATTCTGGT TAGATCTGAT GCAGATGGAT ATGATGAGGA GGTGATGCTG 
AGCCCTGCCA TGCAAGGGGT CATCCTGGCC ATAGCTAAAG CCCGTCAGAC CTTTGACCGA 
GATGGGTCTG AAGCAGGGCT GATTAAGGCA TTCCATGAAG AATACTCCAG GCTCTATCAG 
CTTGCCAAAG AGACCCCCAC CTCTCACAGT GATCCTCGAC TTCAGCATGT CCTTGTCTAC 
TTTTTCCAAA ATGAAGCACC CAAAAGGGTA GTAGAACGAA CCCTTCTGGA ACAGTTTGCA 
GATAAAAATC TTAGCTATGA TGAAAGATCA ATCAGCATTA TGAAGGTGGC TCAAGCGAAA 
CTGAAGGAAA TTGGTCCAGA TGACATGAAT ATGGAAGAGT ACAAGAAGTG GCATGAAGAT 
TATAGTTTGT TCCGAAAAGT GTCTGTGTAT CTCCTAACAG GCCTAGAACT CTATCAAAAA 
GGAAAGTACC AAGAGGCACT TTCCTACCTG GTATATGCCT ACCAGAGCAA TGCTGCCCTG 
CTGATGAAGG GGCCCCGCCG GGGGGTCAAA GAATCCGTGA TTGCTTTATA CCGAAGAAAA 
TGCCTTCTGG AGCTGAATGC CAAAGCAGCT TCTCTTTTTG AAACAAATGA TGATCACTCC 
GTAACTGAGG GCATTAATGT GATGAATGAA CTGATCATCC CCTGCATTCA CCTTATCATT 
AATAATGACA TTTCCAAGGA TGATCTGGAT GCCATTGAGG TCATGAGAAA CCATTGGTGC 
TCTTACCTTG GGCAAGATAT TGCAGAAAAT CTGCAGCTGT GCCTAGGGGA GTTTCTACCC 
AGACTTCTAG ATCCTTCTGC AGAAATCATC GTCTTGAAAG AGCCTCCAAC TATTCGACCC 
AATTCTCCCT ATGACCTATG TAGCCGATTT GCAGCTGTCA TGGAGTCAAT TCAGGGAGTT 
TCAACTGTGA CAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333193.1
CDS	918..3338
Translation	MVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346264.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346264.1

ORF Insert Sequence:

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGCTTAA AGAAGCTGAA 
CCCAAGAAGC CCATGCCCCA GGAAACAAAC CTTGCAGAGC AGTCAGAACA GCCCCCAAAG 
GCTAATGATG CAGAGTCTAC TGCCCAGCCT AATTCTGAGG TCTCTGAAGT CGAGATTCCC 
AGTGTGGGAA GGATTCTGGT TAGATCTGAT GCAGATGGAT ATGATGAGGA GGTGATGCTG 
AGCCCTGCCA TGCAAGGGGT CATCCTGGCC ATAGCTAAAG CCCGTCAGAC CTTTGACCGA 
GATGGGTCTG AAGCAGGGCT GATTAAGGCA TTCCATGAAG AATACTCCAG GCTCTATCAG 
CTTGCCAAAG AGACCCCCAC CTCTCACAGT GATCCTCGAC TTCAGCATGT CCTTGTCTAC 
TTTTTCCAAA ATGAAGCACC CAAAAGGGTA GTAGAACGAA CCCTTCTGGA ACAGTTTGCA 
GATAAAAATC TTAGCTATGA TGAAAGATCA ATCAGCATTA TGAAGGTGGC TCAAGCGAAA 
CTGAAGGAAA TTGGTCCAGA TGACATGAAT ATGGAAGAGT ACAAGAAGTG GCATGAAGAT 
TATAGTTTGT TCCGAAAAGT GTCTGTGTAT CTCCTAACAG GCCTAGAACT CTATCAAAAA 
GGAAAGTACC AAGAGGCACT TTCCTACCTG GTATATGCCT ACCAGAGCAA TGCTGCCCTG 
CTGATGAAGG GGCCCCGCCG GGGGGTCAAA GAATCCGTGA TTGCTTTATA CCGAAGAAAA 
TGCCTTCTGG AGCTGAATGC CAAAGCAGCT TCTCTTTTTG AAACAAATGA TGATCACTCC 
GTAACTGAGG GCATTAATGT GATGAATGAA CTGATCATCC CCTGCATTCA CCTTATCATT 
AATAATGACA TTTCCAAGGA TGATCTGGAT GCCATTGAGG TCATGAGAAA CCATTGGTGC 
TCTTACCTTG GGCAAGATAT TGCAGAAAAT CTGCAGCTGT GCCTAGGGGA GTTTCTACCC 
AGACTTCTAG ATCCTTCTGC AGAAATCATC GTCTTGAAAG AGCCTCCAAC TATTCGACCC 
AATTCTCCCT ATGACCTATG TAGCCGATTT GCAGCTGTCA TGGAGTCAAT TCAGGGAGTT 
TCAACTGTGA CAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346264.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333193.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 14, mRNA.

Price & Availability↑

CloneID	OHu100639
Clone ID Related Accession (Same CDS sequence)	NM_001346264.1 , NM_001346264.2
Accession Version	NM_001346264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform m
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346264.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.41923.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGCTTAA AGAAGCTGAA 
CCCAAGAAGC CCATGCCCCA GGAAACAAAC CTTGCAGAGC AGTCAGAACA GCCCCCAAAG 
GCTAATGATG CAGAGTCTAC TGCCCAGCCT AATTCTGAGG TCTCTGAAGT CGAGATTCCC 
AGTGTGGGAA GGATTCTGGT TAGATCTGAT GCAGATGGAT ATGATGAGGA GGTGATGCTG 
AGCCCTGCCA TGCAAGGGGT CATCCTGGCC ATAGCTAAAG CCCGTCAGAC CTTTGACCGA 
GATGGGTCTG AAGCAGGGCT GATTAAGGCA TTCCATGAAG AATACTCCAG GCTCTATCAG 
CTTGCCAAAG AGACCCCCAC CTCTCACAGT GATCCTCGAC TTCAGCATGT CCTTGTCTAC 
TTTTTCCAAA ATGAAGCACC CAAAAGGGTA GTAGAACGAA CCCTTCTGGA ACAGTTTGCA 
GATAAAAATC TTAGCTATGA TGAAAGATCA ATCAGCATTA TGAAGGTGGC TCAAGCGAAA 
CTGAAGGAAA TTGGTCCAGA TGACATGAAT ATGGAAGAGT ACAAGAAGTG GCATGAAGAT 
TATAGTTTGT TCCGAAAAGT GTCTGTGTAT CTCCTAACAG GCCTAGAACT CTATCAAAAA 
GGAAAGTACC AAGAGGCACT TTCCTACCTG GTATATGCCT ACCAGAGCAA TGCTGCCCTG 
CTGATGAAGG GGCCCCGCCG GGGGGTCAAA GAATCCGTGA TTGCTTTATA CCGAAGAAAA 
TGCCTTCTGG AGCTGAATGC CAAAGCAGCT TCTCTTTTTG AAACAAATGA TGATCACTCC 
GTAACTGAGG GCATTAATGT GATGAATGAA CTGATCATCC CCTGCATTCA CCTTATCATT 
AATAATGACA TTTCCAAGGA TGATCTGGAT GCCATTGAGG TCATGAGAAA CCATTGGTGC 
TCTTACCTTG GGCAAGATAT TGCAGAAAAT CTGCAGCTGT GCCTAGGGGA GTTTCTACCC 
AGACTTCTAG ATCCTTCTGC AGAAATCATC GTCTTGAAAG AGCCTCCAAC TATTCGACCC 
AATTCTCCCT ATGACCTATG TAGCCGATTT GCAGCTGTCA TGGAGTCAAT TCAGGGAGTT 
TCAACTGTGA CAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333193.1
CDS	920..3340
Translation	MVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346264.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346264.2

ORF Insert Sequence:

ATGGTGGAGG GTGATGTTGA GCTTCTTCCC TCCGATCACT CGGTGAAGTA TGGACAAGAG 
CGTTGGTTTA CAAAGCTACC TCCAGTGTTG ACCTTTGAAC TCTCAAGATT TGAGTTTAAT 
CAGTCCCTTG GGCAGCCAGA GAAAATTCAC AATAAGCTGG AATTTCCTCA GATTATTTAT 
ATGGACAGGT ACATGTACAG GAGCAAGGAG CTTATTCGAA ATAAGAGAGA GTGTATTCGA 
AAGTTGAAGG AGGAAATAAA AATTCTGCAG CAAAAATTGG AAAGGTATGT GAAATATGGC 
TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC ATGCTGAAAT ATGTTATTGA ATTTGCTAGT 
ACAAAACCTG CCTCAGAAAG CTGTCCACCT GAAAGTGACA CACATATGAC ATTACCACTT 
TCTTCAGTGC ACTGCTCGGT TTCTGACCAG ACATCCAAGG AAAGTACAAG TACAGAAAGC 
TCTTCTCAGG ATGTTGAAAG TACCTTTTCT TCTCCTGAAG ATTCTTTACC CAAGTCTAAA 
CCACTGACAT CTTCTCGGTC TTCCATGGAA ATGCCTTCAC AGCCAGCTCC ACGAACAGTC 
ACAGATGAGG AGATAAATTT TGTTAAGACC TGTCTTCAGA GATGGAGGAG TGAGATTGAA 
CAAGATATAC AAGATTTAAA GACTTGTATT GCAAGTACTA CTCAGACTAT TGAACAGATG 
TACTGCGATC CTCTCCTTCG TCAGGTGCCT TATCGCTTGC ATGCAGTTCT TGTTCATGAA 
GGACAAGCAA ATGCTGGACA CTATTGGGCC TATATCTATA ATCAACCCCG ACAGAGCTGG 
CTCAAGTACA ATGACATCTC TGTTACTGAA TCTTCCTGGG AAGAAGTTGA AAGAGATTCC 
TATGGAGGCC TGAGAAATGT TAGTGCTTAC TGTCTGATGT ACATTAATGA CAAACTACCC 
TACTTCAATG CAGAGGCAGC CCCAACTGAA TCAGATCAAA TGTCAGAAGT GGAAGCCCTA 
TCTGTGGAAC TCAAGCATTA CATTCAGGAG GATAACTGGC GGTTTGAGCA GGAAGTAGAG 
GAGTGGGAAG AAGAGCAGTC TTGCAAAATC CCTCAAATGG AGTCCTCCAC CAACTCCTCA 
TCACAGGACT ACTCTACATC ACAAGAGCCT TCAGTAGCCT CTTCTCATGG GGTTCGCTGC 
TTGTCGTCTG AGCATGCTGT GATTGTAAAG GAGCAAACTG CCCAGGCTAT TGCAAACACA 
GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA GCGGCACTGA GTGAGCTTAA AGAAGCTGAA 
CCCAAGAAGC CCATGCCCCA GGAAACAAAC CTTGCAGAGC AGTCAGAACA GCCCCCAAAG 
GCTAATGATG CAGAGTCTAC TGCCCAGCCT AATTCTGAGG TCTCTGAAGT CGAGATTCCC 
AGTGTGGGAA GGATTCTGGT TAGATCTGAT GCAGATGGAT ATGATGAGGA GGTGATGCTG 
AGCCCTGCCA TGCAAGGGGT CATCCTGGCC ATAGCTAAAG CCCGTCAGAC CTTTGACCGA 
GATGGGTCTG AAGCAGGGCT GATTAAGGCA TTCCATGAAG AATACTCCAG GCTCTATCAG 
CTTGCCAAAG AGACCCCCAC CTCTCACAGT GATCCTCGAC TTCAGCATGT CCTTGTCTAC 
TTTTTCCAAA ATGAAGCACC CAAAAGGGTA GTAGAACGAA CCCTTCTGGA ACAGTTTGCA 
GATAAAAATC TTAGCTATGA TGAAAGATCA ATCAGCATTA TGAAGGTGGC TCAAGCGAAA 
CTGAAGGAAA TTGGTCCAGA TGACATGAAT ATGGAAGAGT ACAAGAAGTG GCATGAAGAT 
TATAGTTTGT TCCGAAAAGT GTCTGTGTAT CTCCTAACAG GCCTAGAACT CTATCAAAAA 
GGAAAGTACC AAGAGGCACT TTCCTACCTG GTATATGCCT ACCAGAGCAA TGCTGCCCTG 
CTGATGAAGG GGCCCCGCCG GGGGGTCAAA GAATCCGTGA TTGCTTTATA CCGAAGAAAA 
TGCCTTCTGG AGCTGAATGC CAAAGCAGCT TCTCTTTTTG AAACAAATGA TGATCACTCC 
GTAACTGAGG GCATTAATGT GATGAATGAA CTGATCATCC CCTGCATTCA CCTTATCATT 
AATAATGACA TTTCCAAGGA TGATCTGGAT GCCATTGAGG TCATGAGAAA CCATTGGTGC 
TCTTACCTTG GGCAAGATAT TGCAGAAAAT CTGCAGCTGT GCCTAGGGGA GTTTCTACCC 
AGACTTCTAG ATCCTTCTGC AGAAATCATC GTCTTGAAAG AGCCTCCAAC TATTCGACCC 
AATTCTCCCT ATGACCTATG TAGCCGATTT GCAGCTGTCA TGGAGTCAAT TCAGGGAGTT 
TCAACTGTGA CAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346260.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333189.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu100689
Clone ID Related Accession (Same CDS sequence)	NM_001346260.2 , NM_001346260.1
Accession Version	NM_001346260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346260.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.16362.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GCAGTCCCAG GAACAAATCT 
GAAAATCCAA TGGTGCAGCT GTTCTATGGT ACTTTCCTGA CTGAAGGGGT TCGTGAAGGA 
AAACCCTTTT GTAACAATGA GACCTTCGGC CAGTATCCTC TTCAGGTAAA CGGTTATCGC 
AACTTAGACG AGTGTTTGGA AGGGGCCATG GTGGAGGGTG ATGTTGAGCT TCTTCCCTCC 
GATCACTCGG TGAAGTATGG ACAAGAGCGT TGGTTTACAA AGCTACCTCC AGTGTTGACC 
TTTGAACTCT CAAGATTTGA GTTTAATCAG TCCCTTGGGC AGCCAGAGAA AATTCACAAT 
AAGCTGGAAT TTCCTCAGAT TATTTATATG GACAGGTACA TGTACAGGAG CAAGGAGCTT 
ATTCGAAATA AGAGAGAGTG TATTCGAAAG TTGAAGGAGG AAATAAAAAT TCTGCAGCAA 
AAATTGGAAA GGTATGTGAA ATATGGCTCA GGCCCAGCTC GGTTCCCGCT CCCGGACATG 
CTGAAATATG TTATTGAATT TGCTAGTACA AAACCTGCCT CAGAAAGCTG TCCACCTGAA 
AGTGACACAC ATATGACATT ACCACTTTCT TCAGTGCACT GCTCGGTTTC TGACCAGACA 
TCCAAGGAAA GTACAAGTAC AGAAAGCTCT TCTCAGGATG TTGAAAGTAC CTTTTCTTCT 
CCTGAAGATT CTTTACCCAA GTCTAAACCA CTGACATCTT CTCGGTCTTC CATGGAAATG 
CCTTCACAGC CAGCTCCACG AACAGTCACA GATGAGGAGA TAAATTTTGT TAAGACCTGT 
CTTCAGAGAT GGAGGAGTGA GATTGAACAA GATATACAAG ATTTAAAGAC TTGTATTGCA 
AGTACTACTC AGACTATTGA ACAGATGTAC TGCGATCCTC TCCTTCGTCA GGTGCCTTAT 
CGCTTGCATG CAGTTCTTGT TCATGAAGGA CAAGCAAATG CTGGACACTA TTGGGCCTAT 
ATCTATAATC AACCCCGACA GAGCTGGCTC AAGTACAATG ACATCTCTGT TACTGAATCT 
TCCTGGGAAG AAGTTGAAAG AGATTCCTAT GGAGGCCTGA GAAATGTTAG TGCTTACTGT 
CTGATGTACA TTAATGACAA ACTACCCTAC TTCAATGCAG AGGCAGCCCC AACTGAATCA 
GATCAAATGT CAGAAGTGGA AGCCCTATCT GTGGAACTCA AGCATTACAT TCAGGAGGAT 
AACTGGCGGT TTGAGCAGGA AGTAGAGGAG TGGGAAGAAG AGCAGTCTTG CAAAATCCCT 
CAAATGGAGT CCTCCACCAA CTCCTCATCA CAGGACTACT CTACATCACA AGAGCCTTCA 
GTAGCCTCTT CTCATGGGGT TCGCTGCTTG TCGTCTGAGC ATGCTGTGAT TGTAAAGGAG 
CAAACTGCCC AGGCTATTGC AAACACAGCC CGTGCCTATG AGAAGAGCGG TGTAGAAGCG 
GCACTGAGTG AGGTGATGCT GAGCCCTGCC ATGCAAGGGG TCATCCTGGC CATAGCTAAA 
GCCCGTCAGA CCTTTGACCG AGATGGGTCT GAAGCAGGGC TGATTAAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333189.1
CDS	236..3097
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346260.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346260.2

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GCAGTCCCAG GAACAAATCT 
GAAAATCCAA TGGTGCAGCT GTTCTATGGT ACTTTCCTGA CTGAAGGGGT TCGTGAAGGA 
AAACCCTTTT GTAACAATGA GACCTTCGGC CAGTATCCTC TTCAGGTAAA CGGTTATCGC 
AACTTAGACG AGTGTTTGGA AGGGGCCATG GTGGAGGGTG ATGTTGAGCT TCTTCCCTCC 
GATCACTCGG TGAAGTATGG ACAAGAGCGT TGGTTTACAA AGCTACCTCC AGTGTTGACC 
TTTGAACTCT CAAGATTTGA GTTTAATCAG TCCCTTGGGC AGCCAGAGAA AATTCACAAT 
AAGCTGGAAT TTCCTCAGAT TATTTATATG GACAGGTACA TGTACAGGAG CAAGGAGCTT 
ATTCGAAATA AGAGAGAGTG TATTCGAAAG TTGAAGGAGG AAATAAAAAT TCTGCAGCAA 
AAATTGGAAA GGTATGTGAA ATATGGCTCA GGCCCAGCTC GGTTCCCGCT CCCGGACATG 
CTGAAATATG TTATTGAATT TGCTAGTACA AAACCTGCCT CAGAAAGCTG TCCACCTGAA 
AGTGACACAC ATATGACATT ACCACTTTCT TCAGTGCACT GCTCGGTTTC TGACCAGACA 
TCCAAGGAAA GTACAAGTAC AGAAAGCTCT TCTCAGGATG TTGAAAGTAC CTTTTCTTCT 
CCTGAAGATT CTTTACCCAA GTCTAAACCA CTGACATCTT CTCGGTCTTC CATGGAAATG 
CCTTCACAGC CAGCTCCACG AACAGTCACA GATGAGGAGA TAAATTTTGT TAAGACCTGT 
CTTCAGAGAT GGAGGAGTGA GATTGAACAA GATATACAAG ATTTAAAGAC TTGTATTGCA 
AGTACTACTC AGACTATTGA ACAGATGTAC TGCGATCCTC TCCTTCGTCA GGTGCCTTAT 
CGCTTGCATG CAGTTCTTGT TCATGAAGGA CAAGCAAATG CTGGACACTA TTGGGCCTAT 
ATCTATAATC AACCCCGACA GAGCTGGCTC AAGTACAATG ACATCTCTGT TACTGAATCT 
TCCTGGGAAG AAGTTGAAAG AGATTCCTAT GGAGGCCTGA GAAATGTTAG TGCTTACTGT 
CTGATGTACA TTAATGACAA ACTACCCTAC TTCAATGCAG AGGCAGCCCC AACTGAATCA 
GATCAAATGT CAGAAGTGGA AGCCCTATCT GTGGAACTCA AGCATTACAT TCAGGAGGAT 
AACTGGCGGT TTGAGCAGGA AGTAGAGGAG TGGGAAGAAG AGCAGTCTTG CAAAATCCCT 
CAAATGGAGT CCTCCACCAA CTCCTCATCA CAGGACTACT CTACATCACA AGAGCCTTCA 
GTAGCCTCTT CTCATGGGGT TCGCTGCTTG TCGTCTGAGC ATGCTGTGAT TGTAAAGGAG 
CAAACTGCCC AGGCTATTGC AAACACAGCC CGTGCCTATG AGAAGAGCGG TGTAGAAGCG 
GCACTGAGTG AGGTGATGCT GAGCCCTGCC ATGCAAGGGG TCATCCTGGC CATAGCTAAA 
GCCCGTCAGA CCTTTGACCG AGATGGGTCT GAAGCAGGGC TGATTAAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346260.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333189.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu100689
Clone ID Related Accession (Same CDS sequence)	NM_001346260.2 , NM_001346260.1
Accession Version	NM_001346260.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018061.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.16362.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GCAGTCCCAG GAACAAATCT 
GAAAATCCAA TGGTGCAGCT GTTCTATGGT ACTTTCCTGA CTGAAGGGGT TCGTGAAGGA 
AAACCCTTTT GTAACAATGA GACCTTCGGC CAGTATCCTC TTCAGGTAAA CGGTTATCGC 
AACTTAGACG AGTGTTTGGA AGGGGCCATG GTGGAGGGTG ATGTTGAGCT TCTTCCCTCC 
GATCACTCGG TGAAGTATGG ACAAGAGCGT TGGTTTACAA AGCTACCTCC AGTGTTGACC 
TTTGAACTCT CAAGATTTGA GTTTAATCAG TCCCTTGGGC AGCCAGAGAA AATTCACAAT 
AAGCTGGAAT TTCCTCAGAT TATTTATATG GACAGGTACA TGTACAGGAG CAAGGAGCTT 
ATTCGAAATA AGAGAGAGTG TATTCGAAAG TTGAAGGAGG AAATAAAAAT TCTGCAGCAA 
AAATTGGAAA GGTATGTGAA ATATGGCTCA GGCCCAGCTC GGTTCCCGCT CCCGGACATG 
CTGAAATATG TTATTGAATT TGCTAGTACA AAACCTGCCT CAGAAAGCTG TCCACCTGAA 
AGTGACACAC ATATGACATT ACCACTTTCT TCAGTGCACT GCTCGGTTTC TGACCAGACA 
TCCAAGGAAA GTACAAGTAC AGAAAGCTCT TCTCAGGATG TTGAAAGTAC CTTTTCTTCT 
CCTGAAGATT CTTTACCCAA GTCTAAACCA CTGACATCTT CTCGGTCTTC CATGGAAATG 
CCTTCACAGC CAGCTCCACG AACAGTCACA GATGAGGAGA TAAATTTTGT TAAGACCTGT 
CTTCAGAGAT GGAGGAGTGA GATTGAACAA GATATACAAG ATTTAAAGAC TTGTATTGCA 
AGTACTACTC AGACTATTGA ACAGATGTAC TGCGATCCTC TCCTTCGTCA GGTGCCTTAT 
CGCTTGCATG CAGTTCTTGT TCATGAAGGA CAAGCAAATG CTGGACACTA TTGGGCCTAT 
ATCTATAATC AACCCCGACA GAGCTGGCTC AAGTACAATG ACATCTCTGT TACTGAATCT 
TCCTGGGAAG AAGTTGAAAG AGATTCCTAT GGAGGCCTGA GAAATGTTAG TGCTTACTGT 
CTGATGTACA TTAATGACAA ACTACCCTAC TTCAATGCAG AGGCAGCCCC AACTGAATCA 
GATCAAATGT CAGAAGTGGA AGCCCTATCT GTGGAACTCA AGCATTACAT TCAGGAGGAT 
AACTGGCGGT TTGAGCAGGA AGTAGAGGAG TGGGAAGAAG AGCAGTCTTG CAAAATCCCT 
CAAATGGAGT CCTCCACCAA CTCCTCATCA CAGGACTACT CTACATCACA AGAGCCTTCA 
GTAGCCTCTT CTCATGGGGT TCGCTGCTTG TCGTCTGAGC ATGCTGTGAT TGTAAAGGAG 
CAAACTGCCC AGGCTATTGC AAACACAGCC CGTGCCTATG AGAAGAGCGG TGTAGAAGCG 
GCACTGAGTG AGGTGATGCT GAGCCCTGCC ATGCAAGGGG TCATCCTGGC CATAGCTAAA 
GCCCGTCAGA CCTTTGACCG AGATGGGTCT GAAGCAGGGC TGATTAAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333189.1
CDS	234..3095
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346260.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346260.1

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GCAGTCCCAG GAACAAATCT 
GAAAATCCAA TGGTGCAGCT GTTCTATGGT ACTTTCCTGA CTGAAGGGGT TCGTGAAGGA 
AAACCCTTTT GTAACAATGA GACCTTCGGC CAGTATCCTC TTCAGGTAAA CGGTTATCGC 
AACTTAGACG AGTGTTTGGA AGGGGCCATG GTGGAGGGTG ATGTTGAGCT TCTTCCCTCC 
GATCACTCGG TGAAGTATGG ACAAGAGCGT TGGTTTACAA AGCTACCTCC AGTGTTGACC 
TTTGAACTCT CAAGATTTGA GTTTAATCAG TCCCTTGGGC AGCCAGAGAA AATTCACAAT 
AAGCTGGAAT TTCCTCAGAT TATTTATATG GACAGGTACA TGTACAGGAG CAAGGAGCTT 
ATTCGAAATA AGAGAGAGTG TATTCGAAAG TTGAAGGAGG AAATAAAAAT TCTGCAGCAA 
AAATTGGAAA GGTATGTGAA ATATGGCTCA GGCCCAGCTC GGTTCCCGCT CCCGGACATG 
CTGAAATATG TTATTGAATT TGCTAGTACA AAACCTGCCT CAGAAAGCTG TCCACCTGAA 
AGTGACACAC ATATGACATT ACCACTTTCT TCAGTGCACT GCTCGGTTTC TGACCAGACA 
TCCAAGGAAA GTACAAGTAC AGAAAGCTCT TCTCAGGATG TTGAAAGTAC CTTTTCTTCT 
CCTGAAGATT CTTTACCCAA GTCTAAACCA CTGACATCTT CTCGGTCTTC CATGGAAATG 
CCTTCACAGC CAGCTCCACG AACAGTCACA GATGAGGAGA TAAATTTTGT TAAGACCTGT 
CTTCAGAGAT GGAGGAGTGA GATTGAACAA GATATACAAG ATTTAAAGAC TTGTATTGCA 
AGTACTACTC AGACTATTGA ACAGATGTAC TGCGATCCTC TCCTTCGTCA GGTGCCTTAT 
CGCTTGCATG CAGTTCTTGT TCATGAAGGA CAAGCAAATG CTGGACACTA TTGGGCCTAT 
ATCTATAATC AACCCCGACA GAGCTGGCTC AAGTACAATG ACATCTCTGT TACTGAATCT 
TCCTGGGAAG AAGTTGAAAG AGATTCCTAT GGAGGCCTGA GAAATGTTAG TGCTTACTGT 
CTGATGTACA TTAATGACAA ACTACCCTAC TTCAATGCAG AGGCAGCCCC AACTGAATCA 
GATCAAATGT CAGAAGTGGA AGCCCTATCT GTGGAACTCA AGCATTACAT TCAGGAGGAT 
AACTGGCGGT TTGAGCAGGA AGTAGAGGAG TGGGAAGAAG AGCAGTCTTG CAAAATCCCT 
CAAATGGAGT CCTCCACCAA CTCCTCATCA CAGGACTACT CTACATCACA AGAGCCTTCA 
GTAGCCTCTT CTCATGGGGT TCGCTGCTTG TCGTCTGAGC ATGCTGTGAT TGTAAAGGAG 
CAAACTGCCC AGGCTATTGC AAACACAGCC CGTGCCTATG AGAAGAGCGG TGTAGAAGCG 
GCACTGAGTG AGGTGATGCT GAGCCCTGCC ATGCAAGGGG TCATCCTGGC CATAGCTAAA 
GCCCGTCAGA CCTTTGACCG AGATGGGTCT GAAGCAGGGC TGATTAAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346270.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333199.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 19, mRNA.

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CloneID	OHu92401
Clone ID Related Accession (Same CDS sequence)	NM_001346270.1 , NM_001346269.1 , NM_001346271.1 , NM_001346271.2 , NM_001346270.2 , XM_017018067.1 , NM_001346269.2
Accession Version	NM_001346270.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018066.1. Transcript Variant: This variant (19), as well as variants 18 and 20, encodes isoform p. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.234745.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333199.1
CDS	1566..3743
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346270.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346270.1

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346269.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333198.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 18, mRNA.

Price & Availability↑

CloneID	OHu92401
Clone ID Related Accession (Same CDS sequence)	NM_001346270.1 , NM_001346269.1 , NM_001346271.1 , NM_001346271.2 , NM_001346270.2 , XM_017018067.1 , NM_001346269.2
Accession Version	NM_001346269.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. Transcript Variant: This variant (18), as well as variants 19 and 20, encodes isoform p. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.170741.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333198.1
CDS	992..3169
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346269.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346269.1

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346271.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333200.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 20, mRNA.

Price & Availability↑

CloneID	OHu92401
Clone ID Related Accession (Same CDS sequence)	NM_001346270.1 , NM_001346269.1 , NM_001346271.1 , NM_001346271.2 , NM_001346270.2 , XM_017018067.1 , NM_001346269.2
Accession Version	NM_001346271.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018065.1. Transcript Variant: This variant (20), as well as variants 18 and 19, encodes isoform p. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.277625.1, SRR1803615.242126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333200.1
CDS	1203..3380
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346271.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346271.1

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346271.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333200.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 20, mRNA.

Price & Availability↑

CloneID	OHu92401
Clone ID Related Accession (Same CDS sequence)	NM_001346270.1 , NM_001346269.1 , NM_001346271.1 , NM_001346271.2 , NM_001346270.2 , XM_017018067.1 , NM_001346269.2
Accession Version	NM_001346271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346271.1. Transcript Variant: This variant (20), as well as variants 18 and 19, encodes isoform p. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.242126.1, SRR1803612.277625.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333200.1
CDS	1205..3382
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346271.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346271.2

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346270.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333199.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 19, mRNA.

Price & Availability↑

CloneID	OHu92401
Clone ID Related Accession (Same CDS sequence)	NM_001346270.1 , NM_001346269.1 , NM_001346271.1 , NM_001346271.2 , NM_001346270.2 , XM_017018067.1 , NM_001346269.2
Accession Version	NM_001346270.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346270.1. Transcript Variant: This variant (19), as well as variants 18 and 20, encodes isoform p. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.234745.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333199.1
CDS	1568..3745
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346270.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346270.2

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346269.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333198.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 18, mRNA.

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CloneID	OHu92401
Clone ID Related Accession (Same CDS sequence)	NM_001346270.1 , NM_001346269.1 , NM_001346271.1 , NM_001346271.2 , NM_001346270.2 , XM_017018067.1 , NM_001346269.2
Accession Version	NM_001346269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346269.1. Transcript Variant: This variant (18), as well as variants 19 and 20, encodes isoform p. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.170741.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333198.1
CDS	994..3171
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346269.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346269.2

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_017018064.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873553.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X19, mRNA.

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CloneID	OHu92400
Clone ID Related Accession (Same CDS sequence)	XM_017018064.1
Accession Version	XM_017018064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGC TGTTCTATGG TACTTTCCTG ACTGAAGGGG TTCGTGAAGG AAAACCCTTT 
TGTAACAATG AGACCTTCGG CCAGTATCCT CTTCAGGTAA ACGGTTATCG CAACTTAGAC 
GAGTGTTTGG AAGGGGCCAT GGTGGAGGGT GATGTTGAGC TTCTTCCCTC CGATCACTCG 
GTGAAGTATG GACAAGAGCG TTGGTTTACA AAGCTACCTC CAGTGTTGAC CTTTGAACTC 
TCAAGATTTG AGTTTAATCA GTCCCTTGGG CAGCCAGAGA AAATTCACAA TAAGCTGGAA 
TTTCCTCAGA TTATTTATAT GGACAGGTAC ATGTACAGGA GCAAGGAGCT TATTCGAAAT 
AAGAGAGAGT GTATTCGAAA GTTGAAGGAG GAAATAAAAA TTCTGCAGCA AAAATTGGAA 
AGGTATGTGA AATATGGCTC AGGCCCAGCT CGGTTCCCGC TCCCGGACAT GCTGAAATAT 
GTTATTGAAT TTGCTAGTAC AAAACCTGCC TCAGAAAGCT GTCCACCTGA AAGTGACACA 
CATATGACAT TACCACTTTC TTCAGTGCAC TGCTCGGTTT CTGACCAGAC ATCCAAGGAA 
AGTACAAGTA CAGAAAGCTC TTCTCAGGAT GTTGAAAGTA CCTTTTCTTC TCCTGAAGAT 
TCTTTACCCA AGTCTAAACC ACTGACATCT TCTCGGTCTT CCATGGAAAT GCCTTCACAG 
CCAGCTCCAC GAACAGTCAC AGATGAGGAG ATAAATTTTG TTAAGACCTG TCTTCAGAGA 
TGGAGGAGTG AGATTGAACA AGATATACAA GATTTAAAGA CTTGTATTGC AAGTACTACT 
CAGACTATTG AACAGATGTA CTGCGATCCT CTCCTTCGTC AGGTGCCTTA TCGCTTGCAT 
GCAGTTCTTG TTCATGAAGG ACAAGCAAAT GCTGGACACT ATTGGGCCTA TATCTATAAT 
CAACCCCGAC AGAGCTGGCT CAAGTACAAT GACATCTCTG TTACTGAATC TTCCTGGGAA 
GAAGTTGAAA GAGATTCCTA TGGAGGCCTG AGAAATGTTA GTGCTTACTG TCTGATGTAC 
ATTAATGACA AACTACCCTA CTTCAATGCA GAGGCAGCCC CAACTGAATC AGATCAAATG 
TCAGAAGTGG AAGCCCTATC TGTGGAACTC AAGCATTACA TTCAGGAGGA TAACTGGCGG 
TTTGAGCAGG AAGTAGAGGA GTGGGAAGAA GAGCAGTCTT GCAAAATCCC TCAAATGGAG 
TCCTCCACCA ACTCCTCATC ACAGGACTAC TCTACATCAC AAGAGCCTTC AGTAGCCTCT 
TCTCATGGGG TTCGCTGCTT GTCGTCTGAG CATGCTGTGA TTGTAAAGGA GCAAACTGCC 
CAGGCTATTG CAAACACAGC CCGTGCCTAT GAGAAGAGCG GTGTAGAAGC GGCACTGAGT 
GAGGTGATGC TGAGCCCTGC CATGCAAGGG GTCATCCTGG CCATAGCTAA AGCCCGTCAG 
ACCTTTGACC GAGATGGGTC TGAAGCAGGG CTGATTAAGG CATTCCATGA AGAATACTCC 
AGGCTCTATC AGCTTGCCAA AGAGACCCCC ACCTCTCACA GTGATCCTCG ACTTCAGCAT 
GTCCTTGTCT ACTTTTTCCA AAATGAAGCA CCCAAAAGGG TAGTAGAACG AACCCTTCTG 
GAACAGTTTG CAGATAAAAA TCTTAGCTAT GATGAAAGAT CAATCAGCAT TATGAAGGTG 
GCTCAAGCGA AACTGAAGGA AATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG 
TGGCATGAAG ATTATAGTTT GTTCCGAAAA GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA 
CTCTATCAAA AAGGAAAGTA CCAAGAGGCA CTTTCCTACC TGGTATATGC CTACCAGAGC 
AATGCTGCCC TGCTGATGAA GGGGCCCCGC CGGGGGGTCA AAGAATCCGT GATTGCTTTA 
TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTTTT TGAAACAAAT 
GATGATCACT CCGTAACTGA GGGCATTAAT GTGATGAATG AACTGATCAT CCCCTGCATT 
CACCTTATCA TTAATAATGA CATTTCCAAG GATGATCTGG ATGCCATTGA GGTCATGAGA 
AACCATTGGT GCTCTTACCT TGGGCAAGAT ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG 
GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TCGTCTTGAA AGAGCCTCCA 
ACTATTCGAC CCAATTCTCC CTATGACCTA TGTAGCCGAT TTGCAGCTGT CATGGAGTCA 
ATTCAGGGAG TTTCAACTGT GACAGTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873553.1
CDS	728..3100
Translation	MVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_017018064.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018064.1

ORF Insert Sequence:

ATGGTGCAGC TGTTCTATGG TACTTTCCTG ACTGAAGGGG TTCGTGAAGG AAAACCCTTT 
TGTAACAATG AGACCTTCGG CCAGTATCCT CTTCAGGTAA ACGGTTATCG CAACTTAGAC 
GAGTGTTTGG AAGGGGCCAT GGTGGAGGGT GATGTTGAGC TTCTTCCCTC CGATCACTCG 
GTGAAGTATG GACAAGAGCG TTGGTTTACA AAGCTACCTC CAGTGTTGAC CTTTGAACTC 
TCAAGATTTG AGTTTAATCA GTCCCTTGGG CAGCCAGAGA AAATTCACAA TAAGCTGGAA 
TTTCCTCAGA TTATTTATAT GGACAGGTAC ATGTACAGGA GCAAGGAGCT TATTCGAAAT 
AAGAGAGAGT GTATTCGAAA GTTGAAGGAG GAAATAAAAA TTCTGCAGCA AAAATTGGAA 
AGGTATGTGA AATATGGCTC AGGCCCAGCT CGGTTCCCGC TCCCGGACAT GCTGAAATAT 
GTTATTGAAT TTGCTAGTAC AAAACCTGCC TCAGAAAGCT GTCCACCTGA AAGTGACACA 
CATATGACAT TACCACTTTC TTCAGTGCAC TGCTCGGTTT CTGACCAGAC ATCCAAGGAA 
AGTACAAGTA CAGAAAGCTC TTCTCAGGAT GTTGAAAGTA CCTTTTCTTC TCCTGAAGAT 
TCTTTACCCA AGTCTAAACC ACTGACATCT TCTCGGTCTT CCATGGAAAT GCCTTCACAG 
CCAGCTCCAC GAACAGTCAC AGATGAGGAG ATAAATTTTG TTAAGACCTG TCTTCAGAGA 
TGGAGGAGTG AGATTGAACA AGATATACAA GATTTAAAGA CTTGTATTGC AAGTACTACT 
CAGACTATTG AACAGATGTA CTGCGATCCT CTCCTTCGTC AGGTGCCTTA TCGCTTGCAT 
GCAGTTCTTG TTCATGAAGG ACAAGCAAAT GCTGGACACT ATTGGGCCTA TATCTATAAT 
CAACCCCGAC AGAGCTGGCT CAAGTACAAT GACATCTCTG TTACTGAATC TTCCTGGGAA 
GAAGTTGAAA GAGATTCCTA TGGAGGCCTG AGAAATGTTA GTGCTTACTG TCTGATGTAC 
ATTAATGACA AACTACCCTA CTTCAATGCA GAGGCAGCCC CAACTGAATC AGATCAAATG 
TCAGAAGTGG AAGCCCTATC TGTGGAACTC AAGCATTACA TTCAGGAGGA TAACTGGCGG 
TTTGAGCAGG AAGTAGAGGA GTGGGAAGAA GAGCAGTCTT GCAAAATCCC TCAAATGGAG 
TCCTCCACCA ACTCCTCATC ACAGGACTAC TCTACATCAC AAGAGCCTTC AGTAGCCTCT 
TCTCATGGGG TTCGCTGCTT GTCGTCTGAG CATGCTGTGA TTGTAAAGGA GCAAACTGCC 
CAGGCTATTG CAAACACAGC CCGTGCCTAT GAGAAGAGCG GTGTAGAAGC GGCACTGAGT 
GAGGTGATGC TGAGCCCTGC CATGCAAGGG GTCATCCTGG CCATAGCTAA AGCCCGTCAG 
ACCTTTGACC GAGATGGGTC TGAAGCAGGG CTGATTAAGG CATTCCATGA AGAATACTCC 
AGGCTCTATC AGCTTGCCAA AGAGACCCCC ACCTCTCACA GTGATCCTCG ACTTCAGCAT 
GTCCTTGTCT ACTTTTTCCA AAATGAAGCA CCCAAAAGGG TAGTAGAACG AACCCTTCTG 
GAACAGTTTG CAGATAAAAA TCTTAGCTAT GATGAAAGAT CAATCAGCAT TATGAAGGTG 
GCTCAAGCGA AACTGAAGGA AATTGGTCCA GATGACATGA ATATGGAAGA GTACAAGAAG 
TGGCATGAAG ATTATAGTTT GTTCCGAAAA GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA 
CTCTATCAAA AAGGAAAGTA CCAAGAGGCA CTTTCCTACC TGGTATATGC CTACCAGAGC 
AATGCTGCCC TGCTGATGAA GGGGCCCCGC CGGGGGGTCA AAGAATCCGT GATTGCTTTA 
TACCGAAGAA AATGCCTTCT GGAGCTGAAT GCCAAAGCAG CTTCTCTTTT TGAAACAAAT 
GATGATCACT CCGTAACTGA GGGCATTAAT GTGATGAATG AACTGATCAT CCCCTGCATT 
CACCTTATCA TTAATAATGA CATTTCCAAG GATGATCTGG ATGCCATTGA GGTCATGAGA 
AACCATTGGT GCTCTTACCT TGGGCAAGAT ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG 
GAGTTTCTAC CCAGACTTCT AGATCCTTCT GCAGAAATCA TCGTCTTGAA AGAGCCTCCA 
ACTATTCGAC CCAATTCTCC CTATGACCTA TGTAGCCGAT TTGCAGCTGT CATGGAGTCA 
ATTCAGGGAG TTTCAACTGT GACAGTGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_017018067.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873556.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OHu92401
Clone ID Related Accession (Same CDS sequence)	NM_001346270.1 , NM_001346269.1 , NM_001346271.1 , NM_001346271.2 , NM_001346270.2 , XM_017018067.1 , NM_001346269.2
Accession Version	XM_017018067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873556.1
CDS	778..2955
Translation	MRWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_017018067.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018067.1

ORF Insert Sequence:

ATGCGTTGGT TTACAAAGCT ACCTCCAGTG TTGACCTTTG AACTCTCAAG ATTTGAGTTT 
AATCAGTCCC TTGGGCAGCC AGAGAAAATT CACAATAAGC TGGAATTTCC TCAGATTATT 
TATATGGACA GGTACATGTA CAGGAGCAAG GAGCTTATTC GAAATAAGAG AGAGTGTATT 
CGAAAGTTGA AGGAGGAAAT AAAAATTCTG CAGCAAAAAT TGGAAAGGTA TGTGAAATAT 
GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG GACATGCTGA AATATGTTAT TGAATTTGCT 
AGTACAAAAC CTGCCTCAGA AAGCTGTCCA CCTGAAAGTG ACACACATAT GACATTACCA 
CTTTCTTCAG TGCACTGCTC GGTTTCTGAC CAGACATCCA AGGAAAGTAC AAGTACAGAA 
AGCTCTTCTC AGGATGTTGA AAGTACCTTT TCTTCTCCTG AAGATTCTTT ACCCAAGTCT 
AAACCACTGA CATCTTCTCG GTCTTCCATG GAAATGCCTT CACAGCCAGC TCCACGAACA 
GTCACAGATG AGGAGATAAA TTTTGTTAAG ACCTGTCTTC AGAGATGGAG GAGTGAGATT 
GAACAAGATA TACAAGATTT AAAGACTTGT ATTGCAAGTA CTACTCAGAC TATTGAACAG 
ATGTACTGCG ATCCTCTCCT TCGTCAGGTG CCTTATCGCT TGCATGCAGT TCTTGTTCAT 
GAAGGACAAG CAAATGCTGG ACACTATTGG GCCTATATCT ATAATCAACC CCGACAGAGC 
TGGCTCAAGT ACAATGACAT CTCTGTTACT GAATCTTCCT GGGAAGAAGT TGAAAGAGAT 
TCCTATGGAG GCCTGAGAAA TGTTAGTGCT TACTGTCTGA TGTACATTAA TGACAAACTA 
CCCTACTTCA ATGCAGAGGC AGCCCCAACT GAATCAGATC AAATGTCAGA AGTGGAAGCC 
CTATCTGTGG AACTCAAGCA TTACATTCAG GAGGATAACT GGCGGTTTGA GCAGGAAGTA 
GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA ATCCCTCAAA TGGAGTCCTC CACCAACTCC 
TCATCACAGG ACTACTCTAC ATCACAAGAG CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC 
TGCTTGTCGT CTGAGCATGC TGTGATTGTA AAGGAGCAAA CTGCCCAGGC TATTGCAAAC 
ACAGCCCGTG CCTATGAGAA GAGCGGTGTA GAAGCGGCAC TGAGTGAGGT GATGCTGAGC 
CCTGCCATGC AAGGGGTCAT CCTGGCCATA GCTAAAGCCC GTCAGACCTT TGACCGAGAT 
GGGTCTGAAG CAGGGCTGAT TAAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_017018069.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873558.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu92402
Clone ID Related Accession (Same CDS sequence)	XM_017018069.1
Accession Version	XM_017018069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT ATGTTATTGA ATTTGCTAGT ACAAAACCTG CCTCAGAAAG CTGTCCACCT 
GAAAGTGACA CACATATGAC ATTACCACTT TCTTCAGTGC ACTGCTCGGT TTCTGACCAG 
ACATCCAAGG AAAGTACAAG TACAGAAAGC TCTTCTCAGG ATGTTGAAAG TACCTTTTCT 
TCTCCTGAAG ATTCTTTACC CAAGTCTAAA CCACTGACAT CTTCTCGGTC TTCCATGGAA 
ATGCCTTCAC AGCCAGCTCC ACGAACAGTC ACAGATGAGG AGATAAATTT TGTTAAGACC 
TGTCTTCAGA GATGGAGGAG TGAGATTGAA CAAGATATAC AAGATTTAAA GACTTGTATT 
GCAAGTACTA CTCAGACTAT TGAACAGATG TACTGCGATC CTCTCCTTCG TCAGGTGCCT 
TATCGCTTGC ATGCAGTTCT TGTTCATGAA GGACAAGCAA ATGCTGGACA CTATTGGGCC 
TATATCTATA ATCAACCCCG ACAGAGCTGG CTCAAGTACA ATGACATCTC TGTTACTGAA 
TCTTCCTGGG AAGAAGTTGA AAGAGATTCC TATGGAGGCC TGAGAAATGT TAGTGCTTAC 
TGTCTGATGT ACATTAATGA CAAACTACCC TACTTCAATG CAGAGGCAGC CCCAACTGAA 
TCAGATCAAA TGTCAGAAGT GGAAGCCCTA TCTGTGGAAC TCAAGCATTA CATTCAGGAG 
GATAACTGGC GGTTTGAGCA GGAAGTAGAG GAGTGGGAAG AAGAGCAGTC TTGCAAAATC 
CCTCAAATGG AGTCCTCCAC CAACTCCTCA TCACAGGACT ACTCTACATC ACAAGAGCCT 
TCAGTAGCCT CTTCTCATGG GGTTCGCTGC TTGTCGTCTG AGCATGCTGT GATTGTAAAG 
GAGCAAACTG CCCAGGCTAT TGCAAACACA GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA 
GCGGCACTGA GTGAGGCATT CCATGAAGAA TACTCCAGGC TCTATCAGCT TGCCAAAGAG 
ACCCCCACCT CTCACAGTGA TCCTCGACTT CAGCATGTCC TTGTCTACTT TTTCCAAAAT 
GAAGCACCCA AAAGGGTAGT AGAACGAACC CTTCTGGAAC AGTTTGCAGA TAAAAATCTT 
AGCTATGATG AAAGATCAAT CAGCATTATG AAGGTGGCTC AAGCGAAACT GAAGGAAATT 
GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA TAGTTTGTTC 
CGAAAAGTGT CTGTGTATCT CCTAACAGGC CTAGAACTCT ATCAAAAAGG AAAGTACCAA 
GAGGCACTTT CCTACCTGGT ATATGCCTAC CAGAGCAATG CTGCCCTGCT GATGAAGGGG 
CCCCGCCGGG GGGTCAAAGA ATCCGTGATT GCTTTATACC GAAGAAAATG CCTTCTGGTT 
TGTACTGTTT GTAAAGCCTT AAGGTTTTTT TGTTGTTTAC CTCTGCTCCT TGAAGCCAGG 
AAAATATATG GCTTTCAAAA CAGACTATCT CCAACAGGTT GTAGATTTCC CCCCAAAATT 
CTCATGAACA GTTTTACATT GGAACAGGTT TTTTTTCAGT TACTTTTAGT GAATGTCATT 
CTTCTTCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873558.1
CDS	114..1745
Translation	MLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLVCTVCKALRFFCCLPLLLEARKIYGFQNRLSPTGCRFPPKILMNSFTLEQVFFQLLLVNVILLQ

Target ORF information:

RefSeq Version	XM_017018069.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018069.1

ORF Insert Sequence:

ATGCTGAAAT ATGTTATTGA ATTTGCTAGT ACAAAACCTG CCTCAGAAAG CTGTCCACCT 
GAAAGTGACA CACATATGAC ATTACCACTT TCTTCAGTGC ACTGCTCGGT TTCTGACCAG 
ACATCCAAGG AAAGTACAAG TACAGAAAGC TCTTCTCAGG ATGTTGAAAG TACCTTTTCT 
TCTCCTGAAG ATTCTTTACC CAAGTCTAAA CCACTGACAT CTTCTCGGTC TTCCATGGAA 
ATGCCTTCAC AGCCAGCTCC ACGAACAGTC ACAGATGAGG AGATAAATTT TGTTAAGACC 
TGTCTTCAGA GATGGAGGAG TGAGATTGAA CAAGATATAC AAGATTTAAA GACTTGTATT 
GCAAGTACTA CTCAGACTAT TGAACAGATG TACTGCGATC CTCTCCTTCG TCAGGTGCCT 
TATCGCTTGC ATGCAGTTCT TGTTCATGAA GGACAAGCAA ATGCTGGACA CTATTGGGCC 
TATATCTATA ATCAACCCCG ACAGAGCTGG CTCAAGTACA ATGACATCTC TGTTACTGAA 
TCTTCCTGGG AAGAAGTTGA AAGAGATTCC TATGGAGGCC TGAGAAATGT TAGTGCTTAC 
TGTCTGATGT ACATTAATGA CAAACTACCC TACTTCAATG CAGAGGCAGC CCCAACTGAA 
TCAGATCAAA TGTCAGAAGT GGAAGCCCTA TCTGTGGAAC TCAAGCATTA CATTCAGGAG 
GATAACTGGC GGTTTGAGCA GGAAGTAGAG GAGTGGGAAG AAGAGCAGTC TTGCAAAATC 
CCTCAAATGG AGTCCTCCAC CAACTCCTCA TCACAGGACT ACTCTACATC ACAAGAGCCT 
TCAGTAGCCT CTTCTCATGG GGTTCGCTGC TTGTCGTCTG AGCATGCTGT GATTGTAAAG 
GAGCAAACTG CCCAGGCTAT TGCAAACACA GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA 
GCGGCACTGA GTGAGGCATT CCATGAAGAA TACTCCAGGC TCTATCAGCT TGCCAAAGAG 
ACCCCCACCT CTCACAGTGA TCCTCGACTT CAGCATGTCC TTGTCTACTT TTTCCAAAAT 
GAAGCACCCA AAAGGGTAGT AGAACGAACC CTTCTGGAAC AGTTTGCAGA TAAAAATCTT 
AGCTATGATG AAAGATCAAT CAGCATTATG AAGGTGGCTC AAGCGAAACT GAAGGAAATT 
GGTCCAGATG ACATGAATAT GGAAGAGTAC AAGAAGTGGC ATGAAGATTA TAGTTTGTTC 
CGAAAAGTGT CTGTGTATCT CCTAACAGGC CTAGAACTCT ATCAAAAAGG AAAGTACCAA 
GAGGCACTTT CCTACCTGGT ATATGCCTAC CAGAGCAATG CTGCCCTGCT GATGAAGGGG 
CCCCGCCGGG GGGTCAAAGA ATCCGTGATT GCTTTATACC GAAGAAAATG CCTTCTGGTT 
TGTACTGTTT GTAAAGCCTT AAGGTTTTTT TGTTGTTTAC CTCTGCTCCT TGAAGCCAGG 
AAAATATATG GCTTTCAAAA CAGACTATCT CCAACAGGTT GTAGATTTCC CCCCAAAATT 
CTCATGAACA GTTTTACATT GGAACAGGTT TTTTTTCAGT TACTTTTAGT GAATGTCATT 
CTTCTTCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346259.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333188.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu100535
Clone ID Related Accession (Same CDS sequence)	NM_001346259.1 , NM_001346259.2
Accession Version	NM_001346259.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018059.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.61354.1, SRR1803614.55175.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGGCAT TCCATGAAGA ATACTCCAGG CTCTATCAGC TTGCCAAAGA GACCCCCACC 
TCTCACAGTG ATCCTCGACT TCAGCATGTC CTTGTCTACT TTTTCCAAAA TGAAGCACCC 
AAAAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAAAAATCT TAGCTATGAT 
GAAAGATCAA TCAGCATTAT GAAGGTGGCT CAAGCGAAAC TGAAGGAAAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ATAGTTTGTT CCGAAAAGTG 
TCTGTGTATC TCCTAACAGG CCTAGAACTC TATCAAAAAG GAAAGTACCA AGAGGCACTT 
TCCTACCTGG TATATGCCTA CCAGAGCAAT GCTGCCCTGC TGATGAAGGG GCCCCGCCGG 
GGGGTCAAAG AATCCGTGAT TGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTTTTTGA AACAAATGAT GATCACTCCG TAACTGAGGG CATTAATGTG 
ATGAATGAAC TGATCATCCC CTGCATTCAC CTTATCATTA ATAATGACAT TTCCAAGGAT 
GATCTGGATG CCATTGAGGT CATGAGAAAC CATTGGTGCT CTTACCTTGG GCAAGATATT 
GCAGAAAATC TGCAGCTGTG CCTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATCG TCTTGAAAGA GCCTCCAACT ATTCGACCCA ATTCTCCCTA TGACCTATGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAGGGAGTTT CAACTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333188.1
CDS	70..3129
Translation	MTAELQQDDAAGAADGHGSASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346259.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346259.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGGCAT TCCATGAAGA ATACTCCAGG CTCTATCAGC TTGCCAAAGA GACCCCCACC 
TCTCACAGTG ATCCTCGACT TCAGCATGTC CTTGTCTACT TTTTCCAAAA TGAAGCACCC 
AAAAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAAAAATCT TAGCTATGAT 
GAAAGATCAA TCAGCATTAT GAAGGTGGCT CAAGCGAAAC TGAAGGAAAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ATAGTTTGTT CCGAAAAGTG 
TCTGTGTATC TCCTAACAGG CCTAGAACTC TATCAAAAAG GAAAGTACCA AGAGGCACTT 
TCCTACCTGG TATATGCCTA CCAGAGCAAT GCTGCCCTGC TGATGAAGGG GCCCCGCCGG 
GGGGTCAAAG AATCCGTGAT TGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTTTTTGA AACAAATGAT GATCACTCCG TAACTGAGGG CATTAATGTG 
ATGAATGAAC TGATCATCCC CTGCATTCAC CTTATCATTA ATAATGACAT TTCCAAGGAT 
GATCTGGATG CCATTGAGGT CATGAGAAAC CATTGGTGCT CTTACCTTGG GCAAGATATT 
GCAGAAAATC TGCAGCTGTG CCTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATCG TCTTGAAAGA GCCTCCAACT ATTCGACCCA ATTCTCCCTA TGACCTATGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAGGGAGTTT CAACTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346259.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333188.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 9, mRNA.

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CloneID	OHu100535
Clone ID Related Accession (Same CDS sequence)	NM_001346259.1 , NM_001346259.2
Accession Version	NM_001346259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346259.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.61354.1, SRR1803614.55175.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGGCAT TCCATGAAGA ATACTCCAGG CTCTATCAGC TTGCCAAAGA GACCCCCACC 
TCTCACAGTG ATCCTCGACT TCAGCATGTC CTTGTCTACT TTTTCCAAAA TGAAGCACCC 
AAAAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAAAAATCT TAGCTATGAT 
GAAAGATCAA TCAGCATTAT GAAGGTGGCT CAAGCGAAAC TGAAGGAAAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ATAGTTTGTT CCGAAAAGTG 
TCTGTGTATC TCCTAACAGG CCTAGAACTC TATCAAAAAG GAAAGTACCA AGAGGCACTT 
TCCTACCTGG TATATGCCTA CCAGAGCAAT GCTGCCCTGC TGATGAAGGG GCCCCGCCGG 
GGGGTCAAAG AATCCGTGAT TGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTTTTTGA AACAAATGAT GATCACTCCG TAACTGAGGG CATTAATGTG 
ATGAATGAAC TGATCATCCC CTGCATTCAC CTTATCATTA ATAATGACAT TTCCAAGGAT 
GATCTGGATG CCATTGAGGT CATGAGAAAC CATTGGTGCT CTTACCTTGG GCAAGATATT 
GCAGAAAATC TGCAGCTGTG CCTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATCG TCTTGAAAGA GCCTCCAACT ATTCGACCCA ATTCTCCCTA TGACCTATGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAGGGAGTTT CAACTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333188.1
CDS	72..3131
Translation	MTAELQQDDAAGAADGHGSASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346259.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346259.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGGCAT TCCATGAAGA ATACTCCAGG CTCTATCAGC TTGCCAAAGA GACCCCCACC 
TCTCACAGTG ATCCTCGACT TCAGCATGTC CTTGTCTACT TTTTCCAAAA TGAAGCACCC 
AAAAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAAAAATCT TAGCTATGAT 
GAAAGATCAA TCAGCATTAT GAAGGTGGCT CAAGCGAAAC TGAAGGAAAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ATAGTTTGTT CCGAAAAGTG 
TCTGTGTATC TCCTAACAGG CCTAGAACTC TATCAAAAAG GAAAGTACCA AGAGGCACTT 
TCCTACCTGG TATATGCCTA CCAGAGCAAT GCTGCCCTGC TGATGAAGGG GCCCCGCCGG 
GGGGTCAAAG AATCCGTGAT TGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTTTTTGA AACAAATGAT GATCACTCCG TAACTGAGGG CATTAATGTG 
ATGAATGAAC TGATCATCCC CTGCATTCAC CTTATCATTA ATAATGACAT TTCCAAGGAT 
GATCTGGATG CCATTGAGGT CATGAGAAAC CATTGGTGCT CTTACCTTGG GCAAGATATT 
GCAGAAAATC TGCAGCTGTG CCTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATCG TCTTGAAAGA GCCTCCAACT ATTCGACCCA ATTCTCCCTA TGACCTATGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAGGGAGTTT CAACTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346255.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333184.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu100560
Clone ID Related Accession (Same CDS sequence)	NM_001346255.1 , NM_001346255.2
Accession Version	NM_001346255.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018057.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.104038.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAACT ATATTTGTCG TACAAAGCTG GCAGACAGAT ACCCTCAGAG CTGCCAAATG 
CTGTTAAATC AACTGAGAGA AATCACAGGC ATTCAGGACC CTTCCTTTCT CCATGAAGCT 
CTGAAGGCCA GTAATGGTGA CATTACTCAG GCAGTCAGCC TTCTCACTGA TGAGAGAGTT 
AAGGAGCCCA GTCAAGACAC TGTTGCTACA GAACCATCTG AAGTAGAGGG GAGTGCTGCC 
AACAAGGAAG TATTAGCAAA AGTTATAGAC CTTACTCATG ATAACAAAGA TGATCTTCAG 
GCTGCCATTG CTTTGAGTCT ACTGGAGTCT CCCAAAATTC AAGCTGATGG AAGAGATCTT 
AACAGGATGC ATGAAGCAAC CTCTGCAGAA ACTAAACGCT CAAAGAGAAA ACGCTGTGAA 
GTCTGGGGAG AAAACCCCAA TCCCAATGAC TGGAGGAGAG TTGATGGTTG GCCAGTTGGG 
CTGAAAAATG TTGGCAATAC ATGTTGGTTT AGTGCTGTTA TTCAGTCTCT CTTTCAATTG 
CCTGAATTTC GAAGACTTGT TCTCAGTTAT AGTCTGCCAC AAAATGTACT TGAAAATTGT 
CGAAGTCATA CAGAAAAGAG AAATATCATG TTTATGCAAG AGCTTCAGTA TTTGTTTGCT 
CTAATGATGG GATCAAATAG AAAATTTGTA GACCCGTCTG CAGCCCTGGA TCTATTAAAG 
GGAGCATTCC GATCATCTGA GGAACAGCAG CAAGATGTGA GTGAATTCAC ACACAAGCTC 
CTGGATTGGC TAGAGGACGC ATTCCAGCTA GCTGTTAATG TTAACAGTCC CAGGAACAAA 
TCTGAAAATC CAATGGTGCA GCTGTTCTAT GGTACTTTCC TGACTGAAGG GGTTCGTGAA 
GGAAAACCCT TTTGTAACAA TGAGACCTTC GGCCAGTATC CTCTTCAGGT AAACGGTTAT 
CGCAACTTAG ACGAGTGTTT GGAAGGGGCC ATGGTGGAGG GTGATGTTGA GCTTCTTCCC 
TCCGATCACT CGGTGAAGTA TGGACAAGAG CGTTGGTTTA CAAAGCTACC TCCAGTGTTG 
ACCTTTGAAC TCTCAAGATT TGAGTTTAAT CAGTCCCTTG GGCAGCCAGA GAAAATTCAC 
AATAAGCTGG AATTTCCTCA GATTATTTAT ATGGACAGGT ACATGTACAG GAGCAAGGAG 
CTTATTCGAA ATAAGAGAGA GTGTATTCGA AAGTTGAAGG AGGAAATAAA AATTCTGCAG 
CAAAAATTGG AAAGGTATGT GAAATATGGC TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC 
ATGCTGAAAT ATGTTATTGA ATTTGCTAGT ACAAAACCTG CCTCAGAAAG CTGTCCACCT 
GAAAGTGACA CACATATGAC ATTACCACTT TCTTCAGTGC ACTGCTCGGT TTCTGACCAG 
ACATCCAAGG AAAGTACAAG TACAGAAAGC TCTTCTCAGG ATGTTGAAAG TACCTTTTCT 
TCTCCTGAAG ATTCTTTACC CAAGTCTAAA CCACTGACAT CTTCTCGGTC TTCCATGGAA 
ATGCCTTCAC AGCCAGCTCC ACGAACAGTC ACAGATGAGG AGATAAATTT TGTTAAGACC 
TGTCTTCAGA GATGGAGGAG TGAGATTGAA CAAGATATAC AAGATTTAAA GACTTGTATT 
GCAAGTACTA CTCAGACTAT TGAACAGATG TACTGCGATC CTCTCCTTCG TCAGGTGCCT 
TATCGCTTGC ATGCAGTTCT TGTTCATGAA GGACAAGCAA ATGCTGGACA CTATTGGGCC 
TATATCTATA ATCAACCCCG ACAGAGCTGG CTCAAGTACA ATGACATCTC TGTTACTGAA 
TCTTCCTGGG AAGAAGTTGA AAGAGATTCC TATGGAGGCC TGAGAAATGT TAGTGCTTAC 
TGTCTGATGT ACATTAATGA CAAACTACCC TACTTCAATG CAGAGGCAGC CCCAACTGAA 
TCAGATCAAA TGTCAGAAGT GGAAGCCCTA TCTGTGGAAC TCAAGCATTA CATTCAGGAG 
GATAACTGGC GGTTTGAGCA GGAAGTAGAG GAGTGGGAAG AAGAGCAGTC TTGCAAAATC 
CCTCAAATGG AGTCCTCCAC CAACTCCTCA TCACAGGACT ACTCTACATC ACAAGAGCCT 
TCAGTAGCCT CTTCTCATGG GGTTCGCTGC TTGTCGTCTG AGCATGCTGT GATTGTAAAG 
GAGCAAACTG CCCAGGCTAT TGCAAACACA GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA 
GCGGCACTGA GTGAGGTGAT GCTGAGCCCT GCCATGCAAG GGGTCATCCT GGCCATAGCT 
AAAGCCCGTC AGACCTTTGA CCGAGATGGG TCTGAAGCAG GGCTGATTAA GGCATTCCAT 
GAAGAATACT CCAGGCTCTA TCAGCTTGCC AAAGAGACCC CCACCTCTCA CAGTGATCCT 
CGACTTCAGC ATGTCCTTGT CTACTTTTTC CAAAATGAAG CACCCAAAAG GGTAGTAGAA 
CGAACCCTTC TGGAACAGTT TGCAGATAAA AATCTTAGCT ATGATGAAAG ATCAATCAGC 
ATTATGAAGG TGGCTCAAGC GAAACTGAAG GAAATTGGTC CAGATGACAT GAATATGGAA 
GAGTACAAGA AGTGGCATGA AGATTATAGT TTGTTCCGAA AAGTGTCTGT GTATCTCCTA 
ACAGGCCTAG AACTCTATCA AAAAGGAAAG TACCAAGAGG CACTTTCCTA CCTGGTATAT 
GCCTACCAGA GCAATGCTGC CCTGCTGATG AAGGGGCCCC GCCGGGGGGT CAAAGAATCC 
GTGATTGCTT TATACCGAAG AAAATGCCTT CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT 
TTTGAAACAA ATGATGATCA CTCCGTAACT GAGGGCATTA ATGTGATGAA TGAACTGATC 
ATCCCCTGCA TTCACCTTAT CATTAATAAT GACATTTCCA AGGATGATCT GGATGCCATT 
GAGGTCATGA GAAACCATTG GTGCTCTTAC CTTGGGCAAG ATATTGCAGA AAATCTGCAG 
CTGTGCCTAG GGGAGTTTCT ACCCAGACTT CTAGATCCTT CTGCAGAAAT CATCGTCTTG 
AAAGAGCCTC CAACTATTCG ACCCAATTCT CCCTATGACC TATGTAGCCG ATTTGCAGCT 
GTCATGGAGT CAATTCAGGG AGTTTCAACT GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333184.1
CDS	442..3666
Translation	MRNYICRTKLADRYPQSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346255.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346255.1

ORF Insert Sequence:

ATGAGAAACT ATATTTGTCG TACAAAGCTG GCAGACAGAT ACCCTCAGAG CTGCCAAATG 
CTGTTAAATC AACTGAGAGA AATCACAGGC ATTCAGGACC CTTCCTTTCT CCATGAAGCT 
CTGAAGGCCA GTAATGGTGA CATTACTCAG GCAGTCAGCC TTCTCACTGA TGAGAGAGTT 
AAGGAGCCCA GTCAAGACAC TGTTGCTACA GAACCATCTG AAGTAGAGGG GAGTGCTGCC 
AACAAGGAAG TATTAGCAAA AGTTATAGAC CTTACTCATG ATAACAAAGA TGATCTTCAG 
GCTGCCATTG CTTTGAGTCT ACTGGAGTCT CCCAAAATTC AAGCTGATGG AAGAGATCTT 
AACAGGATGC ATGAAGCAAC CTCTGCAGAA ACTAAACGCT CAAAGAGAAA ACGCTGTGAA 
GTCTGGGGAG AAAACCCCAA TCCCAATGAC TGGAGGAGAG TTGATGGTTG GCCAGTTGGG 
CTGAAAAATG TTGGCAATAC ATGTTGGTTT AGTGCTGTTA TTCAGTCTCT CTTTCAATTG 
CCTGAATTTC GAAGACTTGT TCTCAGTTAT AGTCTGCCAC AAAATGTACT TGAAAATTGT 
CGAAGTCATA CAGAAAAGAG AAATATCATG TTTATGCAAG AGCTTCAGTA TTTGTTTGCT 
CTAATGATGG GATCAAATAG AAAATTTGTA GACCCGTCTG CAGCCCTGGA TCTATTAAAG 
GGAGCATTCC GATCATCTGA GGAACAGCAG CAAGATGTGA GTGAATTCAC ACACAAGCTC 
CTGGATTGGC TAGAGGACGC ATTCCAGCTA GCTGTTAATG TTAACAGTCC CAGGAACAAA 
TCTGAAAATC CAATGGTGCA GCTGTTCTAT GGTACTTTCC TGACTGAAGG GGTTCGTGAA 
GGAAAACCCT TTTGTAACAA TGAGACCTTC GGCCAGTATC CTCTTCAGGT AAACGGTTAT 
CGCAACTTAG ACGAGTGTTT GGAAGGGGCC ATGGTGGAGG GTGATGTTGA GCTTCTTCCC 
TCCGATCACT CGGTGAAGTA TGGACAAGAG CGTTGGTTTA CAAAGCTACC TCCAGTGTTG 
ACCTTTGAAC TCTCAAGATT TGAGTTTAAT CAGTCCCTTG GGCAGCCAGA GAAAATTCAC 
AATAAGCTGG AATTTCCTCA GATTATTTAT ATGGACAGGT ACATGTACAG GAGCAAGGAG 
CTTATTCGAA ATAAGAGAGA GTGTATTCGA AAGTTGAAGG AGGAAATAAA AATTCTGCAG 
CAAAAATTGG AAAGGTATGT GAAATATGGC TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC 
ATGCTGAAAT ATGTTATTGA ATTTGCTAGT ACAAAACCTG CCTCAGAAAG CTGTCCACCT 
GAAAGTGACA CACATATGAC ATTACCACTT TCTTCAGTGC ACTGCTCGGT TTCTGACCAG 
ACATCCAAGG AAAGTACAAG TACAGAAAGC TCTTCTCAGG ATGTTGAAAG TACCTTTTCT 
TCTCCTGAAG ATTCTTTACC CAAGTCTAAA CCACTGACAT CTTCTCGGTC TTCCATGGAA 
ATGCCTTCAC AGCCAGCTCC ACGAACAGTC ACAGATGAGG AGATAAATTT TGTTAAGACC 
TGTCTTCAGA GATGGAGGAG TGAGATTGAA CAAGATATAC AAGATTTAAA GACTTGTATT 
GCAAGTACTA CTCAGACTAT TGAACAGATG TACTGCGATC CTCTCCTTCG TCAGGTGCCT 
TATCGCTTGC ATGCAGTTCT TGTTCATGAA GGACAAGCAA ATGCTGGACA CTATTGGGCC 
TATATCTATA ATCAACCCCG ACAGAGCTGG CTCAAGTACA ATGACATCTC TGTTACTGAA 
TCTTCCTGGG AAGAAGTTGA AAGAGATTCC TATGGAGGCC TGAGAAATGT TAGTGCTTAC 
TGTCTGATGT ACATTAATGA CAAACTACCC TACTTCAATG CAGAGGCAGC CCCAACTGAA 
TCAGATCAAA TGTCAGAAGT GGAAGCCCTA TCTGTGGAAC TCAAGCATTA CATTCAGGAG 
GATAACTGGC GGTTTGAGCA GGAAGTAGAG GAGTGGGAAG AAGAGCAGTC TTGCAAAATC 
CCTCAAATGG AGTCCTCCAC CAACTCCTCA TCACAGGACT ACTCTACATC ACAAGAGCCT 
TCAGTAGCCT CTTCTCATGG GGTTCGCTGC TTGTCGTCTG AGCATGCTGT GATTGTAAAG 
GAGCAAACTG CCCAGGCTAT TGCAAACACA GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA 
GCGGCACTGA GTGAGGTGAT GCTGAGCCCT GCCATGCAAG GGGTCATCCT GGCCATAGCT 
AAAGCCCGTC AGACCTTTGA CCGAGATGGG TCTGAAGCAG GGCTGATTAA GGCATTCCAT 
GAAGAATACT CCAGGCTCTA TCAGCTTGCC AAAGAGACCC CCACCTCTCA CAGTGATCCT 
CGACTTCAGC ATGTCCTTGT CTACTTTTTC CAAAATGAAG CACCCAAAAG GGTAGTAGAA 
CGAACCCTTC TGGAACAGTT TGCAGATAAA AATCTTAGCT ATGATGAAAG ATCAATCAGC 
ATTATGAAGG TGGCTCAAGC GAAACTGAAG GAAATTGGTC CAGATGACAT GAATATGGAA 
GAGTACAAGA AGTGGCATGA AGATTATAGT TTGTTCCGAA AAGTGTCTGT GTATCTCCTA 
ACAGGCCTAG AACTCTATCA AAAAGGAAAG TACCAAGAGG CACTTTCCTA CCTGGTATAT 
GCCTACCAGA GCAATGCTGC CCTGCTGATG AAGGGGCCCC GCCGGGGGGT CAAAGAATCC 
GTGATTGCTT TATACCGAAG AAAATGCCTT CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT 
TTTGAAACAA ATGATGATCA CTCCGTAACT GAGGGCATTA ATGTGATGAA TGAACTGATC 
ATCCCCTGCA TTCACCTTAT CATTAATAAT GACATTTCCA AGGATGATCT GGATGCCATT 
GAGGTCATGA GAAACCATTG GTGCTCTTAC CTTGGGCAAG ATATTGCAGA AAATCTGCAG 
CTGTGCCTAG GGGAGTTTCT ACCCAGACTT CTAGATCCTT CTGCAGAAAT CATCGTCTTG 
AAAGAGCCTC CAACTATTCG ACCCAATTCT CCCTATGACC TATGTAGCCG ATTTGCAGCT 
GTCATGGAGT CAATTCAGGG AGTTTCAACT GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346255.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333184.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu100560
Clone ID Related Accession (Same CDS sequence)	NM_001346255.1 , NM_001346255.2
Accession Version	NM_001346255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346255.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.104038.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAACT ATATTTGTCG TACAAAGCTG GCAGACAGAT ACCCTCAGAG CTGCCAAATG 
CTGTTAAATC AACTGAGAGA AATCACAGGC ATTCAGGACC CTTCCTTTCT CCATGAAGCT 
CTGAAGGCCA GTAATGGTGA CATTACTCAG GCAGTCAGCC TTCTCACTGA TGAGAGAGTT 
AAGGAGCCCA GTCAAGACAC TGTTGCTACA GAACCATCTG AAGTAGAGGG GAGTGCTGCC 
AACAAGGAAG TATTAGCAAA AGTTATAGAC CTTACTCATG ATAACAAAGA TGATCTTCAG 
GCTGCCATTG CTTTGAGTCT ACTGGAGTCT CCCAAAATTC AAGCTGATGG AAGAGATCTT 
AACAGGATGC ATGAAGCAAC CTCTGCAGAA ACTAAACGCT CAAAGAGAAA ACGCTGTGAA 
GTCTGGGGAG AAAACCCCAA TCCCAATGAC TGGAGGAGAG TTGATGGTTG GCCAGTTGGG 
CTGAAAAATG TTGGCAATAC ATGTTGGTTT AGTGCTGTTA TTCAGTCTCT CTTTCAATTG 
CCTGAATTTC GAAGACTTGT TCTCAGTTAT AGTCTGCCAC AAAATGTACT TGAAAATTGT 
CGAAGTCATA CAGAAAAGAG AAATATCATG TTTATGCAAG AGCTTCAGTA TTTGTTTGCT 
CTAATGATGG GATCAAATAG AAAATTTGTA GACCCGTCTG CAGCCCTGGA TCTATTAAAG 
GGAGCATTCC GATCATCTGA GGAACAGCAG CAAGATGTGA GTGAATTCAC ACACAAGCTC 
CTGGATTGGC TAGAGGACGC ATTCCAGCTA GCTGTTAATG TTAACAGTCC CAGGAACAAA 
TCTGAAAATC CAATGGTGCA GCTGTTCTAT GGTACTTTCC TGACTGAAGG GGTTCGTGAA 
GGAAAACCCT TTTGTAACAA TGAGACCTTC GGCCAGTATC CTCTTCAGGT AAACGGTTAT 
CGCAACTTAG ACGAGTGTTT GGAAGGGGCC ATGGTGGAGG GTGATGTTGA GCTTCTTCCC 
TCCGATCACT CGGTGAAGTA TGGACAAGAG CGTTGGTTTA CAAAGCTACC TCCAGTGTTG 
ACCTTTGAAC TCTCAAGATT TGAGTTTAAT CAGTCCCTTG GGCAGCCAGA GAAAATTCAC 
AATAAGCTGG AATTTCCTCA GATTATTTAT ATGGACAGGT ACATGTACAG GAGCAAGGAG 
CTTATTCGAA ATAAGAGAGA GTGTATTCGA AAGTTGAAGG AGGAAATAAA AATTCTGCAG 
CAAAAATTGG AAAGGTATGT GAAATATGGC TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC 
ATGCTGAAAT ATGTTATTGA ATTTGCTAGT ACAAAACCTG CCTCAGAAAG CTGTCCACCT 
GAAAGTGACA CACATATGAC ATTACCACTT TCTTCAGTGC ACTGCTCGGT TTCTGACCAG 
ACATCCAAGG AAAGTACAAG TACAGAAAGC TCTTCTCAGG ATGTTGAAAG TACCTTTTCT 
TCTCCTGAAG ATTCTTTACC CAAGTCTAAA CCACTGACAT CTTCTCGGTC TTCCATGGAA 
ATGCCTTCAC AGCCAGCTCC ACGAACAGTC ACAGATGAGG AGATAAATTT TGTTAAGACC 
TGTCTTCAGA GATGGAGGAG TGAGATTGAA CAAGATATAC AAGATTTAAA GACTTGTATT 
GCAAGTACTA CTCAGACTAT TGAACAGATG TACTGCGATC CTCTCCTTCG TCAGGTGCCT 
TATCGCTTGC ATGCAGTTCT TGTTCATGAA GGACAAGCAA ATGCTGGACA CTATTGGGCC 
TATATCTATA ATCAACCCCG ACAGAGCTGG CTCAAGTACA ATGACATCTC TGTTACTGAA 
TCTTCCTGGG AAGAAGTTGA AAGAGATTCC TATGGAGGCC TGAGAAATGT TAGTGCTTAC 
TGTCTGATGT ACATTAATGA CAAACTACCC TACTTCAATG CAGAGGCAGC CCCAACTGAA 
TCAGATCAAA TGTCAGAAGT GGAAGCCCTA TCTGTGGAAC TCAAGCATTA CATTCAGGAG 
GATAACTGGC GGTTTGAGCA GGAAGTAGAG GAGTGGGAAG AAGAGCAGTC TTGCAAAATC 
CCTCAAATGG AGTCCTCCAC CAACTCCTCA TCACAGGACT ACTCTACATC ACAAGAGCCT 
TCAGTAGCCT CTTCTCATGG GGTTCGCTGC TTGTCGTCTG AGCATGCTGT GATTGTAAAG 
GAGCAAACTG CCCAGGCTAT TGCAAACACA GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA 
GCGGCACTGA GTGAGGTGAT GCTGAGCCCT GCCATGCAAG GGGTCATCCT GGCCATAGCT 
AAAGCCCGTC AGACCTTTGA CCGAGATGGG TCTGAAGCAG GGCTGATTAA GGCATTCCAT 
GAAGAATACT CCAGGCTCTA TCAGCTTGCC AAAGAGACCC CCACCTCTCA CAGTGATCCT 
CGACTTCAGC ATGTCCTTGT CTACTTTTTC CAAAATGAAG CACCCAAAAG GGTAGTAGAA 
CGAACCCTTC TGGAACAGTT TGCAGATAAA AATCTTAGCT ATGATGAAAG ATCAATCAGC 
ATTATGAAGG TGGCTCAAGC GAAACTGAAG GAAATTGGTC CAGATGACAT GAATATGGAA 
GAGTACAAGA AGTGGCATGA AGATTATAGT TTGTTCCGAA AAGTGTCTGT GTATCTCCTA 
ACAGGCCTAG AACTCTATCA AAAAGGAAAG TACCAAGAGG CACTTTCCTA CCTGGTATAT 
GCCTACCAGA GCAATGCTGC CCTGCTGATG AAGGGGCCCC GCCGGGGGGT CAAAGAATCC 
GTGATTGCTT TATACCGAAG AAAATGCCTT CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT 
TTTGAAACAA ATGATGATCA CTCCGTAACT GAGGGCATTA ATGTGATGAA TGAACTGATC 
ATCCCCTGCA TTCACCTTAT CATTAATAAT GACATTTCCA AGGATGATCT GGATGCCATT 
GAGGTCATGA GAAACCATTG GTGCTCTTAC CTTGGGCAAG ATATTGCAGA AAATCTGCAG 
CTGTGCCTAG GGGAGTTTCT ACCCAGACTT CTAGATCCTT CTGCAGAAAT CATCGTCTTG 
AAAGAGCCTC CAACTATTCG ACCCAATTCT CCCTATGACC TATGTAGCCG ATTTGCAGCT 
GTCATGGAGT CAATTCAGGG AGTTTCAACT GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333184.1
CDS	444..3668
Translation	MRNYICRTKLADRYPQSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346255.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346255.2

ORF Insert Sequence:

ATGAGAAACT ATATTTGTCG TACAAAGCTG GCAGACAGAT ACCCTCAGAG CTGCCAAATG 
CTGTTAAATC AACTGAGAGA AATCACAGGC ATTCAGGACC CTTCCTTTCT CCATGAAGCT 
CTGAAGGCCA GTAATGGTGA CATTACTCAG GCAGTCAGCC TTCTCACTGA TGAGAGAGTT 
AAGGAGCCCA GTCAAGACAC TGTTGCTACA GAACCATCTG AAGTAGAGGG GAGTGCTGCC 
AACAAGGAAG TATTAGCAAA AGTTATAGAC CTTACTCATG ATAACAAAGA TGATCTTCAG 
GCTGCCATTG CTTTGAGTCT ACTGGAGTCT CCCAAAATTC AAGCTGATGG AAGAGATCTT 
AACAGGATGC ATGAAGCAAC CTCTGCAGAA ACTAAACGCT CAAAGAGAAA ACGCTGTGAA 
GTCTGGGGAG AAAACCCCAA TCCCAATGAC TGGAGGAGAG TTGATGGTTG GCCAGTTGGG 
CTGAAAAATG TTGGCAATAC ATGTTGGTTT AGTGCTGTTA TTCAGTCTCT CTTTCAATTG 
CCTGAATTTC GAAGACTTGT TCTCAGTTAT AGTCTGCCAC AAAATGTACT TGAAAATTGT 
CGAAGTCATA CAGAAAAGAG AAATATCATG TTTATGCAAG AGCTTCAGTA TTTGTTTGCT 
CTAATGATGG GATCAAATAG AAAATTTGTA GACCCGTCTG CAGCCCTGGA TCTATTAAAG 
GGAGCATTCC GATCATCTGA GGAACAGCAG CAAGATGTGA GTGAATTCAC ACACAAGCTC 
CTGGATTGGC TAGAGGACGC ATTCCAGCTA GCTGTTAATG TTAACAGTCC CAGGAACAAA 
TCTGAAAATC CAATGGTGCA GCTGTTCTAT GGTACTTTCC TGACTGAAGG GGTTCGTGAA 
GGAAAACCCT TTTGTAACAA TGAGACCTTC GGCCAGTATC CTCTTCAGGT AAACGGTTAT 
CGCAACTTAG ACGAGTGTTT GGAAGGGGCC ATGGTGGAGG GTGATGTTGA GCTTCTTCCC 
TCCGATCACT CGGTGAAGTA TGGACAAGAG CGTTGGTTTA CAAAGCTACC TCCAGTGTTG 
ACCTTTGAAC TCTCAAGATT TGAGTTTAAT CAGTCCCTTG GGCAGCCAGA GAAAATTCAC 
AATAAGCTGG AATTTCCTCA GATTATTTAT ATGGACAGGT ACATGTACAG GAGCAAGGAG 
CTTATTCGAA ATAAGAGAGA GTGTATTCGA AAGTTGAAGG AGGAAATAAA AATTCTGCAG 
CAAAAATTGG AAAGGTATGT GAAATATGGC TCAGGCCCAG CTCGGTTCCC GCTCCCGGAC 
ATGCTGAAAT ATGTTATTGA ATTTGCTAGT ACAAAACCTG CCTCAGAAAG CTGTCCACCT 
GAAAGTGACA CACATATGAC ATTACCACTT TCTTCAGTGC ACTGCTCGGT TTCTGACCAG 
ACATCCAAGG AAAGTACAAG TACAGAAAGC TCTTCTCAGG ATGTTGAAAG TACCTTTTCT 
TCTCCTGAAG ATTCTTTACC CAAGTCTAAA CCACTGACAT CTTCTCGGTC TTCCATGGAA 
ATGCCTTCAC AGCCAGCTCC ACGAACAGTC ACAGATGAGG AGATAAATTT TGTTAAGACC 
TGTCTTCAGA GATGGAGGAG TGAGATTGAA CAAGATATAC AAGATTTAAA GACTTGTATT 
GCAAGTACTA CTCAGACTAT TGAACAGATG TACTGCGATC CTCTCCTTCG TCAGGTGCCT 
TATCGCTTGC ATGCAGTTCT TGTTCATGAA GGACAAGCAA ATGCTGGACA CTATTGGGCC 
TATATCTATA ATCAACCCCG ACAGAGCTGG CTCAAGTACA ATGACATCTC TGTTACTGAA 
TCTTCCTGGG AAGAAGTTGA AAGAGATTCC TATGGAGGCC TGAGAAATGT TAGTGCTTAC 
TGTCTGATGT ACATTAATGA CAAACTACCC TACTTCAATG CAGAGGCAGC CCCAACTGAA 
TCAGATCAAA TGTCAGAAGT GGAAGCCCTA TCTGTGGAAC TCAAGCATTA CATTCAGGAG 
GATAACTGGC GGTTTGAGCA GGAAGTAGAG GAGTGGGAAG AAGAGCAGTC TTGCAAAATC 
CCTCAAATGG AGTCCTCCAC CAACTCCTCA TCACAGGACT ACTCTACATC ACAAGAGCCT 
TCAGTAGCCT CTTCTCATGG GGTTCGCTGC TTGTCGTCTG AGCATGCTGT GATTGTAAAG 
GAGCAAACTG CCCAGGCTAT TGCAAACACA GCCCGTGCCT ATGAGAAGAG CGGTGTAGAA 
GCGGCACTGA GTGAGGTGAT GCTGAGCCCT GCCATGCAAG GGGTCATCCT GGCCATAGCT 
AAAGCCCGTC AGACCTTTGA CCGAGATGGG TCTGAAGCAG GGCTGATTAA GGCATTCCAT 
GAAGAATACT CCAGGCTCTA TCAGCTTGCC AAAGAGACCC CCACCTCTCA CAGTGATCCT 
CGACTTCAGC ATGTCCTTGT CTACTTTTTC CAAAATGAAG CACCCAAAAG GGTAGTAGAA 
CGAACCCTTC TGGAACAGTT TGCAGATAAA AATCTTAGCT ATGATGAAAG ATCAATCAGC 
ATTATGAAGG TGGCTCAAGC GAAACTGAAG GAAATTGGTC CAGATGACAT GAATATGGAA 
GAGTACAAGA AGTGGCATGA AGATTATAGT TTGTTCCGAA AAGTGTCTGT GTATCTCCTA 
ACAGGCCTAG AACTCTATCA AAAAGGAAAG TACCAAGAGG CACTTTCCTA CCTGGTATAT 
GCCTACCAGA GCAATGCTGC CCTGCTGATG AAGGGGCCCC GCCGGGGGGT CAAAGAATCC 
GTGATTGCTT TATACCGAAG AAAATGCCTT CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT 
TTTGAAACAA ATGATGATCA CTCCGTAACT GAGGGCATTA ATGTGATGAA TGAACTGATC 
ATCCCCTGCA TTCACCTTAT CATTAATAAT GACATTTCCA AGGATGATCT GGATGCCATT 
GAGGTCATGA GAAACCATTG GTGCTCTTAC CTTGGGCAAG ATATTGCAGA AAATCTGCAG 
CTGTGCCTAG GGGAGTTTCT ACCCAGACTT CTAGATCCTT CTGCAGAAAT CATCGTCTTG 
AAAGAGCCTC CAACTATTCG ACCCAATTCT CCCTATGACC TATGTAGCCG ATTTGCAGCT 
GTCATGGAGT CAATTCAGGG AGTTTCAACT GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346253.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333182.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu100584
Clone ID Related Accession (Same CDS sequence)	NM_001346253.2 , NM_001346253.1
Accession Version	NM_001346253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346253.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.245736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGCTTA AAGAAGCTGA ACCCAAGAAG CCCATGCCCC AGGAAACAAA CCTTGCAGAG 
CAGTCAGAAC AGCCCCCAAA GGCTAATGAT GCAGAGTCTA CTGCCCAGCC TAATTCTGAG 
GTCTCTGAAG TCGAGATTCC CAGTGTGGGA AGGATTCTGG TTAGATCTGA TGCAGATGGA 
TATGATGAGG AGGTGATGCT GAGCCCTGCC ATGCAAGGGG TCATCCTGGC CATAGCTAAA 
GCCCGTCAGA CCTTTGACCG AGATGGGTCT GAAGCAGGGC TGATTAAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333182.1
CDS	72..3413
Translation	MTAELQQDDAAGAADGHGSASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346253.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346253.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGCTTA AAGAAGCTGA ACCCAAGAAG CCCATGCCCC AGGAAACAAA CCTTGCAGAG 
CAGTCAGAAC AGCCCCCAAA GGCTAATGAT GCAGAGTCTA CTGCCCAGCC TAATTCTGAG 
GTCTCTGAAG TCGAGATTCC CAGTGTGGGA AGGATTCTGG TTAGATCTGA TGCAGATGGA 
TATGATGAGG AGGTGATGCT GAGCCCTGCC ATGCAAGGGG TCATCCTGGC CATAGCTAAA 
GCCCGTCAGA CCTTTGACCG AGATGGGTCT GAAGCAGGGC TGATTAAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346253.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333182.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu100584
Clone ID Related Accession (Same CDS sequence)	NM_001346253.2 , NM_001346253.1
Accession Version	NM_001346253.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018056.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.245736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGCTTA AAGAAGCTGA ACCCAAGAAG CCCATGCCCC AGGAAACAAA CCTTGCAGAG 
CAGTCAGAAC AGCCCCCAAA GGCTAATGAT GCAGAGTCTA CTGCCCAGCC TAATTCTGAG 
GTCTCTGAAG TCGAGATTCC CAGTGTGGGA AGGATTCTGG TTAGATCTGA TGCAGATGGA 
TATGATGAGG AGGTGATGCT GAGCCCTGCC ATGCAAGGGG TCATCCTGGC CATAGCTAAA 
GCCCGTCAGA CCTTTGACCG AGATGGGTCT GAAGCAGGGC TGATTAAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333182.1
CDS	70..3411
Translation	MTAELQQDDAAGAADGHGSASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346253.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346253.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGCTTA AAGAAGCTGA ACCCAAGAAG CCCATGCCCC AGGAAACAAA CCTTGCAGAG 
CAGTCAGAAC AGCCCCCAAA GGCTAATGAT GCAGAGTCTA CTGCCCAGCC TAATTCTGAG 
GTCTCTGAAG TCGAGATTCC CAGTGTGGGA AGGATTCTGG TTAGATCTGA TGCAGATGGA 
TATGATGAGG AGGTGATGCT GAGCCCTGCC ATGCAAGGGG TCATCCTGGC CATAGCTAAA 
GCCCGTCAGA CCTTTGACCG AGATGGGTCT GAAGCAGGGC TGATTAAGGC ATTCCATGAA 
GAATACTCCA GGCTCTATCA GCTTGCCAAA GAGACCCCCA CCTCTCACAG TGATCCTCGA 
CTTCAGCATG TCCTTGTCTA CTTTTTCCAA AATGAAGCAC CCAAAAGGGT AGTAGAACGA 
ACCCTTCTGG AACAGTTTGC AGATAAAAAT CTTAGCTATG ATGAAAGATC AATCAGCATT 
ATGAAGGTGG CTCAAGCGAA ACTGAAGGAA ATTGGTCCAG ATGACATGAA TATGGAAGAG 
TACAAGAAGT GGCATGAAGA TTATAGTTTG TTCCGAAAAG TGTCTGTGTA TCTCCTAACA 
GGCCTAGAAC TCTATCAAAA AGGAAAGTAC CAAGAGGCAC TTTCCTACCT GGTATATGCC 
TACCAGAGCA ATGCTGCCCT GCTGATGAAG GGGCCCCGCC GGGGGGTCAA AGAATCCGTG 
ATTGCTTTAT ACCGAAGAAA ATGCCTTCTG GAGCTGAATG CCAAAGCAGC TTCTCTTTTT 
GAAACAAATG ATGATCACTC CGTAACTGAG GGCATTAATG TGATGAATGA ACTGATCATC 
CCCTGCATTC ACCTTATCAT TAATAATGAC ATTTCCAAGG ATGATCTGGA TGCCATTGAG 
GTCATGAGAA ACCATTGGTG CTCTTACCTT GGGCAAGATA TTGCAGAAAA TCTGCAGCTG 
TGCCTAGGGG AGTTTCTACC CAGACTTCTA GATCCTTCTG CAGAAATCAT CGTCTTGAAA 
GAGCCTCCAA CTATTCGACC CAATTCTCCC TATGACCTAT GTAGCCGATT TGCAGCTGTC 
ATGGAGTCAA TTCAGGGAGT TTCAACTGTG ACAGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346257.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333186.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 7, mRNA.

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CloneID	OHu100646
Clone ID Related Accession (Same CDS sequence)	NM_001346257.1 , NM_001346257.2
Accession Version	NM_001346257.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_017018058.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.236138.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGGTGA TGCTGAGCCC TGCCATGCAA GGGGTCATCC TGGCCATAGC TAAAGCCCGT 
CAGACCTTTG ACCGAGATGG GTCTGAAGCA GGGCTGATTA AGGCATTCCA TGAAGAATAC 
TCCAGGCTCT ATCAGCTTGC CAAAGAGACC CCCACCTCTC ACAGTGATCC TCGACTTCAG 
CATGTCCTTG TCTACTTTTT CCAAAATGAA GCACCCAAAA GGGTAGTAGA ACGAACCCTT 
CTGGAACAGT TTGCAGATAA AAATCTTAGC TATGATGAAA GATCAATCAG CATTATGAAG 
GTGGCTCAAG CGAAACTGAA GGAAATTGGT CCAGATGACA TGAATATGGA AGAGTACAAG 
AAGTGGCATG AAGATTATAG TTTGTTCCGA AAAGTGTCTG TGTATCTCCT AACAGGCCTA 
GAACTCTATC AAAAAGGAAA GTACCAAGAG GCACTTTCCT ACCTGGTATA TGCCTACCAG 
AGCAATGCTG CCCTGCTGAT GAAGGGGCCC CGCCGGGGGG TCAAAGAATC CGTGATTGCT 
TTATACCGAA GAAAATGCCT TCTGGAGCTG AATGCCAAAG CAGCTTCTCT TTTTGAAACA 
AATGATGATC ACTCCGTAAC TGAGGGCATT AATGTGATGA ATGAACTGAT CATCCCCTGC 
ATTCACCTTA TCATTAATAA TGACATTTCC AAGGATGATC TGGATGCCAT TGAGGTCATG 
AGAAACCATT GGTGCTCTTA CCTTGGGCAA GATATTGCAG AAAATCTGCA GCTGTGCCTA 
GGGGAGTTTC TACCCAGACT TCTAGATCCT TCTGCAGAAA TCATCGTCTT GAAAGAGCCT 
CCAACTATTC GACCCAATTC TCCCTATGAC CTATGTAGCC GATTTGCAGC TGTCATGGAG 
TCAATTCAGG GAGTTTCAAC TGTGACAGTG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333186.1
CDS	70..3225
Translation	MTAELQQDDAAGAADGHGSASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346257.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346257.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGGTGA TGCTGAGCCC TGCCATGCAA GGGGTCATCC TGGCCATAGC TAAAGCCCGT 
CAGACCTTTG ACCGAGATGG GTCTGAAGCA GGGCTGATTA AGGCATTCCA TGAAGAATAC 
TCCAGGCTCT ATCAGCTTGC CAAAGAGACC CCCACCTCTC ACAGTGATCC TCGACTTCAG 
CATGTCCTTG TCTACTTTTT CCAAAATGAA GCACCCAAAA GGGTAGTAGA ACGAACCCTT 
CTGGAACAGT TTGCAGATAA AAATCTTAGC TATGATGAAA GATCAATCAG CATTATGAAG 
GTGGCTCAAG CGAAACTGAA GGAAATTGGT CCAGATGACA TGAATATGGA AGAGTACAAG 
AAGTGGCATG AAGATTATAG TTTGTTCCGA AAAGTGTCTG TGTATCTCCT AACAGGCCTA 
GAACTCTATC AAAAAGGAAA GTACCAAGAG GCACTTTCCT ACCTGGTATA TGCCTACCAG 
AGCAATGCTG CCCTGCTGAT GAAGGGGCCC CGCCGGGGGG TCAAAGAATC CGTGATTGCT 
TTATACCGAA GAAAATGCCT TCTGGAGCTG AATGCCAAAG CAGCTTCTCT TTTTGAAACA 
AATGATGATC ACTCCGTAAC TGAGGGCATT AATGTGATGA ATGAACTGAT CATCCCCTGC 
ATTCACCTTA TCATTAATAA TGACATTTCC AAGGATGATC TGGATGCCAT TGAGGTCATG 
AGAAACCATT GGTGCTCTTA CCTTGGGCAA GATATTGCAG AAAATCTGCA GCTGTGCCTA 
GGGGAGTTTC TACCCAGACT TCTAGATCCT TCTGCAGAAA TCATCGTCTT GAAAGAGCCT 
CCAACTATTC GACCCAATTC TCCCTATGAC CTATGTAGCC GATTTGCAGC TGTCATGGAG 
TCAATTCAGG GAGTTTCAAC TGTGACAGTG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346257.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333186.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 7, mRNA.

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CloneID	OHu100646
Clone ID Related Accession (Same CDS sequence)	NM_001346257.1 , NM_001346257.2
Accession Version	NM_001346257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346257.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.236138.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGGTGA TGCTGAGCCC TGCCATGCAA GGGGTCATCC TGGCCATAGC TAAAGCCCGT 
CAGACCTTTG ACCGAGATGG GTCTGAAGCA GGGCTGATTA AGGCATTCCA TGAAGAATAC 
TCCAGGCTCT ATCAGCTTGC CAAAGAGACC CCCACCTCTC ACAGTGATCC TCGACTTCAG 
CATGTCCTTG TCTACTTTTT CCAAAATGAA GCACCCAAAA GGGTAGTAGA ACGAACCCTT 
CTGGAACAGT TTGCAGATAA AAATCTTAGC TATGATGAAA GATCAATCAG CATTATGAAG 
GTGGCTCAAG CGAAACTGAA GGAAATTGGT CCAGATGACA TGAATATGGA AGAGTACAAG 
AAGTGGCATG AAGATTATAG TTTGTTCCGA AAAGTGTCTG TGTATCTCCT AACAGGCCTA 
GAACTCTATC AAAAAGGAAA GTACCAAGAG GCACTTTCCT ACCTGGTATA TGCCTACCAG 
AGCAATGCTG CCCTGCTGAT GAAGGGGCCC CGCCGGGGGG TCAAAGAATC CGTGATTGCT 
TTATACCGAA GAAAATGCCT TCTGGAGCTG AATGCCAAAG CAGCTTCTCT TTTTGAAACA 
AATGATGATC ACTCCGTAAC TGAGGGCATT AATGTGATGA ATGAACTGAT CATCCCCTGC 
ATTCACCTTA TCATTAATAA TGACATTTCC AAGGATGATC TGGATGCCAT TGAGGTCATG 
AGAAACCATT GGTGCTCTTA CCTTGGGCAA GATATTGCAG AAAATCTGCA GCTGTGCCTA 
GGGGAGTTTC TACCCAGACT TCTAGATCCT TCTGCAGAAA TCATCGTCTT GAAAGAGCCT 
CCAACTATTC GACCCAATTC TCCCTATGAC CTATGTAGCC GATTTGCAGC TGTCATGGAG 
TCAATTCAGG GAGTTTCAAC TGTGACAGTG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333186.1
CDS	72..3227
Translation	MTAELQQDDAAGAADGHGSASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346257.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346257.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGTC CCAGGAACAA ATCTGAAAAT 
CCAATGGTGC AGCTGTTCTA TGGTACTTTC CTGACTGAAG GGGTTCGTGA AGGAAAACCC 
TTTTGTAACA ATGAGACCTT CGGCCAGTAT CCTCTTCAGG TAAACGGTTA TCGCAACTTA 
GACGAGTGTT TGGAAGGGGC CATGGTGGAG GGTGATGTTG AGCTTCTTCC CTCCGATCAC 
TCGGTGAAGT ATGGACAAGA GCGTTGGTTT ACAAAGCTAC CTCCAGTGTT GACCTTTGAA 
CTCTCAAGAT TTGAGTTTAA TCAGTCCCTT GGGCAGCCAG AGAAAATTCA CAATAAGCTG 
GAATTTCCTC AGATTATTTA TATGGACAGG TACATGTACA GGAGCAAGGA GCTTATTCGA 
AATAAGAGAG AGTGTATTCG AAAGTTGAAG GAGGAAATAA AAATTCTGCA GCAAAAATTG 
GAAAGGTATG TGAAATATGG CTCAGGCCCA GCTCGGTTCC CGCTCCCGGA CATGCTGAAA 
TATGTTATTG AATTTGCTAG TACAAAACCT GCCTCAGAAA GCTGTCCACC TGAAAGTGAC 
ACACATATGA CATTACCACT TTCTTCAGTG CACTGCTCGG TTTCTGACCA GACATCCAAG 
GAAAGTACAA GTACAGAAAG CTCTTCTCAG GATGTTGAAA GTACCTTTTC TTCTCCTGAA 
GATTCTTTAC CCAAGTCTAA ACCACTGACA TCTTCTCGGT CTTCCATGGA AATGCCTTCA 
CAGCCAGCTC CACGAACAGT CACAGATGAG GAGATAAATT TTGTTAAGAC CTGTCTTCAG 
AGATGGAGGA GTGAGATTGA ACAAGATATA CAAGATTTAA AGACTTGTAT TGCAAGTACT 
ACTCAGACTA TTGAACAGAT GTACTGCGAT CCTCTCCTTC GTCAGGTGCC TTATCGCTTG 
CATGCAGTTC TTGTTCATGA AGGACAAGCA AATGCTGGAC ACTATTGGGC CTATATCTAT 
AATCAACCCC GACAGAGCTG GCTCAAGTAC AATGACATCT CTGTTACTGA ATCTTCCTGG 
GAAGAAGTTG AAAGAGATTC CTATGGAGGC CTGAGAAATG TTAGTGCTTA CTGTCTGATG 
TACATTAATG ACAAACTACC CTACTTCAAT GCAGAGGCAG CCCCAACTGA ATCAGATCAA 
ATGTCAGAAG TGGAAGCCCT ATCTGTGGAA CTCAAGCATT ACATTCAGGA GGATAACTGG 
CGGTTTGAGC AGGAAGTAGA GGAGTGGGAA GAAGAGCAGT CTTGCAAAAT CCCTCAAATG 
GAGTCCTCCA CCAACTCCTC ATCACAGGAC TACTCTACAT CACAAGAGCC TTCAGTAGCC 
TCTTCTCATG GGGTTCGCTG CTTGTCGTCT GAGCATGCTG TGATTGTAAA GGAGCAAACT 
GCCCAGGCTA TTGCAAACAC AGCCCGTGCC TATGAGAAGA GCGGTGTAGA AGCGGCACTG 
AGTGAGGTGA TGCTGAGCCC TGCCATGCAA GGGGTCATCC TGGCCATAGC TAAAGCCCGT 
CAGACCTTTG ACCGAGATGG GTCTGAAGCA GGGCTGATTA AGGCATTCCA TGAAGAATAC 
TCCAGGCTCT ATCAGCTTGC CAAAGAGACC CCCACCTCTC ACAGTGATCC TCGACTTCAG 
CATGTCCTTG TCTACTTTTT CCAAAATGAA GCACCCAAAA GGGTAGTAGA ACGAACCCTT 
CTGGAACAGT TTGCAGATAA AAATCTTAGC TATGATGAAA GATCAATCAG CATTATGAAG 
GTGGCTCAAG CGAAACTGAA GGAAATTGGT CCAGATGACA TGAATATGGA AGAGTACAAG 
AAGTGGCATG AAGATTATAG TTTGTTCCGA AAAGTGTCTG TGTATCTCCT AACAGGCCTA 
GAACTCTATC AAAAAGGAAA GTACCAAGAG GCACTTTCCT ACCTGGTATA TGCCTACCAG 
AGCAATGCTG CCCTGCTGAT GAAGGGGCCC CGCCGGGGGG TCAAAGAATC CGTGATTGCT 
TTATACCGAA GAAAATGCCT TCTGGAGCTG AATGCCAAAG CAGCTTCTCT TTTTGAAACA 
AATGATGATC ACTCCGTAAC TGAGGGCATT AATGTGATGA ATGAACTGAT CATCCCCTGC 
ATTCACCTTA TCATTAATAA TGACATTTCC AAGGATGATC TGGATGCCAT TGAGGTCATG 
AGAAACCATT GGTGCTCTTA CCTTGGGCAA GATATTGCAG AAAATCTGCA GCTGTGCCTA 
GGGGAGTTTC TACCCAGACT TCTAGATCCT TCTGCAGAAA TCATCGTCTT GAAAGAGCCT 
CCAACTATTC GACCCAATTC TCCCTATGAC CTATGTAGCC GATTTGCAGC TGTCATGGAG 
TCAATTCAGG GAGTTTCAAC TGTGACAGTG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_017018060.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873549.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu92399
Clone ID Related Accession (Same CDS sequence)	XM_017018060.1
Accession Version	XM_017018060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGC TTAAAGAAGC TGAACCCAAG AAGCCCATGC CCCAGGAAAC AAACCTTGCA 
GAGCAGTCAG AACAGCCCCC AAAGGCTAAT GATGCAGAGT CTACTGCCCA GCCTAATTCT 
GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG GGAAGGATTC TGGTTAGATC TGATGCAGAT 
GGATATGATG AGGAGGTGAT GCTGAGCCCT GCCATGCAAG GGGTCATCCT GGCCATAGCT 
AAAGCCCGTC AGACCTTTGA CCGAGATGGG TCTGAAGCAG GGCTGATTAA GGCATTCCAT 
GAAGAATACT CCAGGCTCTA TCAGCTTGCC AAAGAGACCC CCACCTCTCA CAGTGATCCT 
CGACTTCAGC ATGTCCTTGT CTACTTTTTC CAAAATGAAG CACCCAAAAG GGTAGTAGAA 
CGAACCCTTC TGGAACAGTT TGCAGATAAA AATCTTAGCT ATGATGAAAG ATCAATCAGC 
ATTATGAAGG TGGCTCAAGC GAAACTGAAG GAAATTGGTC CAGATGACAT GAATATGGAA 
GAGTACAAGA AGTGGCATGA AGATTATAGT TTGTTCCGAA AAGTGTCTGT GTATCTCCTA 
ACAGGCCTAG AACTCTATCA AAAAGGAAAG TACCAAGAGG CACTTTCCTA CCTGGTATAT 
GCCTACCAGA GCAATGCTGC CCTGCTGATG AAGGGGCCCC GCCGGGGGGT CAAAGAATCC 
GTGATTGCTT TATACCGAAG AAAATGCCTT CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT 
TTTGAAACAA ATGATGATCA CTCCGTAACT GAGGGCATTA ATGTGATGAA TGAACTGATC 
ATCCCCTGCA TTCACCTTAT CATTAATAAT GACATTTCCA AGGATGATCT GGATGCCATT 
GAGGTCATGA GAAACCATTG GTGCTCTTAC CTTGGGCAAG ATATTGCAGA AAATCTGCAG 
CTGTGCCTAG GGGAGTTTCT ACCCAGACTT CTAGATCCTT CTGCAGAAAT CATCGTCTTG 
AAAGAGCCTC CAACTATTCG ACCCAATTCT CCCTATGACC TATGTAGCCG ATTTGCAGCT 
GTCATGGAGT CAATTCAGGG AGTTTCAACT GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873549.1
CDS	234..3278
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_017018060.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018060.1

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGC TTAAAGAAGC TGAACCCAAG AAGCCCATGC CCCAGGAAAC AAACCTTGCA 
GAGCAGTCAG AACAGCCCCC AAAGGCTAAT GATGCAGAGT CTACTGCCCA GCCTAATTCT 
GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG GGAAGGATTC TGGTTAGATC TGATGCAGAT 
GGATATGATG AGGAGGTGAT GCTGAGCCCT GCCATGCAAG GGGTCATCCT GGCCATAGCT 
AAAGCCCGTC AGACCTTTGA CCGAGATGGG TCTGAAGCAG GGCTGATTAA GGCATTCCAT 
GAAGAATACT CCAGGCTCTA TCAGCTTGCC AAAGAGACCC CCACCTCTCA CAGTGATCCT 
CGACTTCAGC ATGTCCTTGT CTACTTTTTC CAAAATGAAG CACCCAAAAG GGTAGTAGAA 
CGAACCCTTC TGGAACAGTT TGCAGATAAA AATCTTAGCT ATGATGAAAG ATCAATCAGC 
ATTATGAAGG TGGCTCAAGC GAAACTGAAG GAAATTGGTC CAGATGACAT GAATATGGAA 
GAGTACAAGA AGTGGCATGA AGATTATAGT TTGTTCCGAA AAGTGTCTGT GTATCTCCTA 
ACAGGCCTAG AACTCTATCA AAAAGGAAAG TACCAAGAGG CACTTTCCTA CCTGGTATAT 
GCCTACCAGA GCAATGCTGC CCTGCTGATG AAGGGGCCCC GCCGGGGGGT CAAAGAATCC 
GTGATTGCTT TATACCGAAG AAAATGCCTT CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT 
TTTGAAACAA ATGATGATCA CTCCGTAACT GAGGGCATTA ATGTGATGAA TGAACTGATC 
ATCCCCTGCA TTCACCTTAT CATTAATAAT GACATTTCCA AGGATGATCT GGATGCCATT 
GAGGTCATGA GAAACCATTG GTGCTCTTAC CTTGGGCAAG ATATTGCAGA AAATCTGCAG 
CTGTGCCTAG GGGAGTTTCT ACCCAGACTT CTAGATCCTT CTGCAGAAAT CATCGTCTTG 
AAAGAGCCTC CAACTATTCG ACCCAATTCT CCCTATGACC TATGTAGCCG ATTTGCAGCT 
GTCATGGAGT CAATTCAGGG AGTTTCAACT GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_020886.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065937.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03574
Clone ID Related Accession (Same CDS sequence)	NM_020886.4 , NM_020886.3
Accession Version	NM_020886.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, DB061316.1, AF266283.1, AP003170.4, BC035986.1 and BF434481.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_020886.3. Transcript Variant: This variant (1) encodes isoform a (also known as isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.66634.1, SRR1803617.245823.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGGTGATG CTGAGCCCTG CCATGCAAGG GGTCATCCTG 
GCCATAGCTA AAGCCCGTCA GACCTTTGAC CGAGATGGGT CTGAAGCAGG GCTGATTAAG 
GCATTCCATG AAGAATACTC CAGGCTCTAT CAGCTTGCCA AAGAGACCCC CACCTCTCAC 
AGTGATCCTC GACTTCAGCA TGTCCTTGTC TACTTTTTCC AAAATGAAGC ACCCAAAAGG 
GTAGTAGAAC GAACCCTTCT GGAACAGTTT GCAGATAAAA ATCTTAGCTA TGATGAAAGA 
TCAATCAGCA TTATGAAGGT GGCTCAAGCG AAACTGAAGG AAATTGGTCC AGATGACATG 
AATATGGAAG AGTACAAGAA GTGGCATGAA GATTATAGTT TGTTCCGAAA AGTGTCTGTG 
TATCTCCTAA CAGGCCTAGA ACTCTATCAA AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC 
CTGGTATATG CCTACCAGAG CAATGCTGCC CTGCTGATGA AGGGGCCCCG CCGGGGGGTC 
AAAGAATCCG TGATTGCTTT ATACCGAAGA AAATGCCTTC TGGAGCTGAA TGCCAAAGCA 
GCTTCTCTTT TTGAAACAAA TGATGATCAC TCCGTAACTG AGGGCATTAA TGTGATGAAT 
GAACTGATCA TCCCCTGCAT TCACCTTATC ATTAATAATG ACATTTCCAA GGATGATCTG 
GATGCCATTG AGGTCATGAG AAACCATTGG TGCTCTTACC TTGGGCAAGA TATTGCAGAA 
AATCTGCAGC TGTGCCTAGG GGAGTTTCTA CCCAGACTTC TAGATCCTTC TGCAGAAATC 
ATCGTCTTGA AAGAGCCTCC AACTATTCGA CCCAATTCTC CCTATGACCT ATGTAGCCGA 
TTTGCAGCTG TCATGGAGTC AATTCAGGGA GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065937.1
CDS	72..3305
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_020886.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020886.4

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGGTGATG CTGAGCCCTG CCATGCAAGG GGTCATCCTG 
GCCATAGCTA AAGCCCGTCA GACCTTTGAC CGAGATGGGT CTGAAGCAGG GCTGATTAAG 
GCATTCCATG AAGAATACTC CAGGCTCTAT CAGCTTGCCA AAGAGACCCC CACCTCTCAC 
AGTGATCCTC GACTTCAGCA TGTCCTTGTC TACTTTTTCC AAAATGAAGC ACCCAAAAGG 
GTAGTAGAAC GAACCCTTCT GGAACAGTTT GCAGATAAAA ATCTTAGCTA TGATGAAAGA 
TCAATCAGCA TTATGAAGGT GGCTCAAGCG AAACTGAAGG AAATTGGTCC AGATGACATG 
AATATGGAAG AGTACAAGAA GTGGCATGAA GATTATAGTT TGTTCCGAAA AGTGTCTGTG 
TATCTCCTAA CAGGCCTAGA ACTCTATCAA AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC 
CTGGTATATG CCTACCAGAG CAATGCTGCC CTGCTGATGA AGGGGCCCCG CCGGGGGGTC 
AAAGAATCCG TGATTGCTTT ATACCGAAGA AAATGCCTTC TGGAGCTGAA TGCCAAAGCA 
GCTTCTCTTT TTGAAACAAA TGATGATCAC TCCGTAACTG AGGGCATTAA TGTGATGAAT 
GAACTGATCA TCCCCTGCAT TCACCTTATC ATTAATAATG ACATTTCCAA GGATGATCTG 
GATGCCATTG AGGTCATGAG AAACCATTGG TGCTCTTACC TTGGGCAAGA TATTGCAGAA 
AATCTGCAGC TGTGCCTAGG GGAGTTTCTA CCCAGACTTC TAGATCCTTC TGCAGAAATC 
ATCGTCTTGA AAGAGCCTCC AACTATTCGA CCCAATTCTC CCTATGACCT ATGTAGCCGA 
TTTGCAGCTG TCATGGAGTC AATTCAGGGA GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_020886.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065937.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03574
Clone ID Related Accession (Same CDS sequence)	NM_020886.4 , NM_020886.3
Accession Version	NM_020886.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB061316.1, AF266283.1, AP003170.4, BC035986.1 and BF434481.1. This sequence is a reference standard in the RefSeqGene project. On Jul 18, 2014 this sequence version replaced NM_020886.2. Transcript Variant: This variant (1) encodes isoform a (also known as isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.220742.1, SRR1803614.185392.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGGTGATG CTGAGCCCTG CCATGCAAGG GGTCATCCTG 
GCCATAGCTA AAGCCCGTCA GACCTTTGAC CGAGATGGGT CTGAAGCAGG GCTGATTAAG 
GCATTCCATG AAGAATACTC CAGGCTCTAT CAGCTTGCCA AAGAGACCCC CACCTCTCAC 
AGTGATCCTC GACTTCAGCA TGTCCTTGTC TACTTTTTCC AAAATGAAGC ACCCAAAAGG 
GTAGTAGAAC GAACCCTTCT GGAACAGTTT GCAGATAAAA ATCTTAGCTA TGATGAAAGA 
TCAATCAGCA TTATGAAGGT GGCTCAAGCG AAACTGAAGG AAATTGGTCC AGATGACATG 
AATATGGAAG AGTACAAGAA GTGGCATGAA GATTATAGTT TGTTCCGAAA AGTGTCTGTG 
TATCTCCTAA CAGGCCTAGA ACTCTATCAA AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC 
CTGGTATATG CCTACCAGAG CAATGCTGCC CTGCTGATGA AGGGGCCCCG CCGGGGGGTC 
AAAGAATCCG TGATTGCTTT ATACCGAAGA AAATGCCTTC TGGAGCTGAA TGCCAAAGCA 
GCTTCTCTTT TTGAAACAAA TGATGATCAC TCCGTAACTG AGGGCATTAA TGTGATGAAT 
GAACTGATCA TCCCCTGCAT TCACCTTATC ATTAATAATG ACATTTCCAA GGATGATCTG 
GATGCCATTG AGGTCATGAG AAACCATTGG TGCTCTTACC TTGGGCAAGA TATTGCAGAA 
AATCTGCAGC TGTGCCTAGG GGAGTTTCTA CCCAGACTTC TAGATCCTTC TGCAGAAATC 
ATCGTCTTGA AAGAGCCTCC AACTATTCGA CCCAATTCTC CCTATGACCT ATGTAGCCGA 
TTTGCAGCTG TCATGGAGTC AATTCAGGGA GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065937.1
CDS	70..3303
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_020886.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020886.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGGTGATG CTGAGCCCTG CCATGCAAGG GGTCATCCTG 
GCCATAGCTA AAGCCCGTCA GACCTTTGAC CGAGATGGGT CTGAAGCAGG GCTGATTAAG 
GCATTCCATG AAGAATACTC CAGGCTCTAT CAGCTTGCCA AAGAGACCCC CACCTCTCAC 
AGTGATCCTC GACTTCAGCA TGTCCTTGTC TACTTTTTCC AAAATGAAGC ACCCAAAAGG 
GTAGTAGAAC GAACCCTTCT GGAACAGTTT GCAGATAAAA ATCTTAGCTA TGATGAAAGA 
TCAATCAGCA TTATGAAGGT GGCTCAAGCG AAACTGAAGG AAATTGGTCC AGATGACATG 
AATATGGAAG AGTACAAGAA GTGGCATGAA GATTATAGTT TGTTCCGAAA AGTGTCTGTG 
TATCTCCTAA CAGGCCTAGA ACTCTATCAA AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC 
CTGGTATATG CCTACCAGAG CAATGCTGCC CTGCTGATGA AGGGGCCCCG CCGGGGGGTC 
AAAGAATCCG TGATTGCTTT ATACCGAAGA AAATGCCTTC TGGAGCTGAA TGCCAAAGCA 
GCTTCTCTTT TTGAAACAAA TGATGATCAC TCCGTAACTG AGGGCATTAA TGTGATGAAT 
GAACTGATCA TCCCCTGCAT TCACCTTATC ATTAATAATG ACATTTCCAA GGATGATCTG 
GATGCCATTG AGGTCATGAG AAACCATTGG TGCTCTTACC TTGGGCAAGA TATTGCAGAA 
AATCTGCAGC TGTGCCTAGG GGAGTTTCTA CCCAGACTTC TAGATCCTTC TGCAGAAATC 
ATCGTCTTGA AAGAGCCTCC AACTATTCGA CCCAATTCTC CCTATGACCT ATGTAGCCGA 
TTTGCAGCTG TCATGGAGTC AATTCAGGGA GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346252.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333181.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 3, mRNA.

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CloneID	OHu45845
Clone ID Related Accession (Same CDS sequence)	NM_001346252.2 , NM_001346252.1
Accession Version	NM_001346252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346252.1. Transcript Variant: This variant (3) encodes the longest isoform (c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.39353.1, SRR1660805.48038.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGCTTAAA GAAGCTGAAC CCAAGAAGCC CATGCCCCAG 
GAAACAAACC TTGCAGAGCA GTCAGAACAG CCCCCAAAGG CTAATGATGC AGAGTCTACT 
GCCCAGCCTA ATTCTGAGGT CTCTGAAGTC GAGATTCCCA GTGTGGGAAG GATTCTGGTT 
AGATCTGATG CAGATGGATA TGATGAGGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC 
ATCCTGGCCA TAGCTAAAGC CCGTCAGACC TTTGACCGAG ATGGGTCTGA AGCAGGGCTG 
ATTAAGGCAT TCCATGAAGA ATACTCCAGG CTCTATCAGC TTGCCAAAGA GACCCCCACC 
TCTCACAGTG ATCCTCGACT TCAGCATGTC CTTGTCTACT TTTTCCAAAA TGAAGCACCC 
AAAAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAAAAATCT TAGCTATGAT 
GAAAGATCAA TCAGCATTAT GAAGGTGGCT CAAGCGAAAC TGAAGGAAAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ATAGTTTGTT CCGAAAAGTG 
TCTGTGTATC TCCTAACAGG CCTAGAACTC TATCAAAAAG GAAAGTACCA AGAGGCACTT 
TCCTACCTGG TATATGCCTA CCAGAGCAAT GCTGCCCTGC TGATGAAGGG GCCCCGCCGG 
GGGGTCAAAG AATCCGTGAT TGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTTTTTGA AACAAATGAT GATCACTCCG TAACTGAGGG CATTAATGTG 
ATGAATGAAC TGATCATCCC CTGCATTCAC CTTATCATTA ATAATGACAT TTCCAAGGAT 
GATCTGGATG CCATTGAGGT CATGAGAAAC CATTGGTGCT CTTACCTTGG GCAAGATATT 
GCAGAAAATC TGCAGCTGTG CCTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATCG TCTTGAAAGA GCCTCCAACT ATTCGACCCA ATTCTCCCTA TGACCTATGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAGGGAGTTT CAACTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333181.1
CDS	72..3491
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346252.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346252.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGCTTAAA GAAGCTGAAC CCAAGAAGCC CATGCCCCAG 
GAAACAAACC TTGCAGAGCA GTCAGAACAG CCCCCAAAGG CTAATGATGC AGAGTCTACT 
GCCCAGCCTA ATTCTGAGGT CTCTGAAGTC GAGATTCCCA GTGTGGGAAG GATTCTGGTT 
AGATCTGATG CAGATGGATA TGATGAGGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC 
ATCCTGGCCA TAGCTAAAGC CCGTCAGACC TTTGACCGAG ATGGGTCTGA AGCAGGGCTG 
ATTAAGGCAT TCCATGAAGA ATACTCCAGG CTCTATCAGC TTGCCAAAGA GACCCCCACC 
TCTCACAGTG ATCCTCGACT TCAGCATGTC CTTGTCTACT TTTTCCAAAA TGAAGCACCC 
AAAAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAAAAATCT TAGCTATGAT 
GAAAGATCAA TCAGCATTAT GAAGGTGGCT CAAGCGAAAC TGAAGGAAAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ATAGTTTGTT CCGAAAAGTG 
TCTGTGTATC TCCTAACAGG CCTAGAACTC TATCAAAAAG GAAAGTACCA AGAGGCACTT 
TCCTACCTGG TATATGCCTA CCAGAGCAAT GCTGCCCTGC TGATGAAGGG GCCCCGCCGG 
GGGGTCAAAG AATCCGTGAT TGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTTTTTGA AACAAATGAT GATCACTCCG TAACTGAGGG CATTAATGTG 
ATGAATGAAC TGATCATCCC CTGCATTCAC CTTATCATTA ATAATGACAT TTCCAAGGAT 
GATCTGGATG CCATTGAGGT CATGAGAAAC CATTGGTGCT CTTACCTTGG GCAAGATATT 
GCAGAAAATC TGCAGCTGTG CCTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATCG TCTTGAAAGA GCCTCCAACT ATTCGACCCA ATTCTCCCTA TGACCTATGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAGGGAGTTT CAACTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346252.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333181.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu45845
Clone ID Related Accession (Same CDS sequence)	NM_001346252.2 , NM_001346252.1
Accession Version	NM_001346252.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_005271631.2. Transcript Variant: This variant (3) encodes the longest isoform (c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.39353.1, SRR1660805.48038.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGCTTAAA GAAGCTGAAC CCAAGAAGCC CATGCCCCAG 
GAAACAAACC TTGCAGAGCA GTCAGAACAG CCCCCAAAGG CTAATGATGC AGAGTCTACT 
GCCCAGCCTA ATTCTGAGGT CTCTGAAGTC GAGATTCCCA GTGTGGGAAG GATTCTGGTT 
AGATCTGATG CAGATGGATA TGATGAGGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC 
ATCCTGGCCA TAGCTAAAGC CCGTCAGACC TTTGACCGAG ATGGGTCTGA AGCAGGGCTG 
ATTAAGGCAT TCCATGAAGA ATACTCCAGG CTCTATCAGC TTGCCAAAGA GACCCCCACC 
TCTCACAGTG ATCCTCGACT TCAGCATGTC CTTGTCTACT TTTTCCAAAA TGAAGCACCC 
AAAAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAAAAATCT TAGCTATGAT 
GAAAGATCAA TCAGCATTAT GAAGGTGGCT CAAGCGAAAC TGAAGGAAAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ATAGTTTGTT CCGAAAAGTG 
TCTGTGTATC TCCTAACAGG CCTAGAACTC TATCAAAAAG GAAAGTACCA AGAGGCACTT 
TCCTACCTGG TATATGCCTA CCAGAGCAAT GCTGCCCTGC TGATGAAGGG GCCCCGCCGG 
GGGGTCAAAG AATCCGTGAT TGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTTTTTGA AACAAATGAT GATCACTCCG TAACTGAGGG CATTAATGTG 
ATGAATGAAC TGATCATCCC CTGCATTCAC CTTATCATTA ATAATGACAT TTCCAAGGAT 
GATCTGGATG CCATTGAGGT CATGAGAAAC CATTGGTGCT CTTACCTTGG GCAAGATATT 
GCAGAAAATC TGCAGCTGTG CCTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATCG TCTTGAAAGA GCCTCCAACT ATTCGACCCA ATTCTCCCTA TGACCTATGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAGGGAGTTT CAACTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333181.1
CDS	70..3489
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346252.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346252.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGCTTAAA GAAGCTGAAC CCAAGAAGCC CATGCCCCAG 
GAAACAAACC TTGCAGAGCA GTCAGAACAG CCCCCAAAGG CTAATGATGC AGAGTCTACT 
GCCCAGCCTA ATTCTGAGGT CTCTGAAGTC GAGATTCCCA GTGTGGGAAG GATTCTGGTT 
AGATCTGATG CAGATGGATA TGATGAGGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC 
ATCCTGGCCA TAGCTAAAGC CCGTCAGACC TTTGACCGAG ATGGGTCTGA AGCAGGGCTG 
ATTAAGGCAT TCCATGAAGA ATACTCCAGG CTCTATCAGC TTGCCAAAGA GACCCCCACC 
TCTCACAGTG ATCCTCGACT TCAGCATGTC CTTGTCTACT TTTTCCAAAA TGAAGCACCC 
AAAAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAAAAATCT TAGCTATGAT 
GAAAGATCAA TCAGCATTAT GAAGGTGGCT CAAGCGAAAC TGAAGGAAAT TGGTCCAGAT 
GACATGAATA TGGAAGAGTA CAAGAAGTGG CATGAAGATT ATAGTTTGTT CCGAAAAGTG 
TCTGTGTATC TCCTAACAGG CCTAGAACTC TATCAAAAAG GAAAGTACCA AGAGGCACTT 
TCCTACCTGG TATATGCCTA CCAGAGCAAT GCTGCCCTGC TGATGAAGGG GCCCCGCCGG 
GGGGTCAAAG AATCCGTGAT TGCTTTATAC CGAAGAAAAT GCCTTCTGGA GCTGAATGCC 
AAAGCAGCTT CTCTTTTTGA AACAAATGAT GATCACTCCG TAACTGAGGG CATTAATGTG 
ATGAATGAAC TGATCATCCC CTGCATTCAC CTTATCATTA ATAATGACAT TTCCAAGGAT 
GATCTGGATG CCATTGAGGT CATGAGAAAC CATTGGTGCT CTTACCTTGG GCAAGATATT 
GCAGAAAATC TGCAGCTGTG CCTAGGGGAG TTTCTACCCA GACTTCTAGA TCCTTCTGCA 
GAAATCATCG TCTTGAAAGA GCCTCCAACT ATTCGACCCA ATTCTCCCTA TGACCTATGT 
AGCCGATTTG CAGCTGTCAT GGAGTCAATT CAGGGAGTTT CAACTGTGAC AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346254.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333183.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 5, mRNA.

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CloneID	OHu45849
Clone ID Related Accession (Same CDS sequence)	NM_001346254.2 , NM_001346254.1
Accession Version	NM_001346254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346254.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.198394.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGGTAT GTGAAATATG GCTCAGGCCC AGCTCGGTTC 
CCGCTCCCGG ACATGCTGAA ATATGTTATT GAATTTGCTA GTACAAAACC TGCCTCAGAA 
AGCTGTCCAC CTGAAAGTGA CACACATATG ACATTACCAC TTTCTTCAGT GCACTGCTCG 
GTTTCTGACC AGACATCCAA GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC 
CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTGATT 
AAGGCATTCC ATGAAGAATA CTCCAGGCTC TATCAGCTTG CCAAAGAGAC CCCCACCTCT 
CACAGTGATC CTCGACTTCA GCATGTCCTT GTCTACTTTT TCCAAAATGA AGCACCCAAA 
AGGGTAGTAG AACGAACCCT TCTGGAACAG TTTGCAGATA AAAATCTTAG CTATGATGAA 
AGATCAATCA GCATTATGAA GGTGGCTCAA GCGAAACTGA AGGAAATTGG TCCAGATGAC 
ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTATA GTTTGTTCCG AAAAGTGTCT 
GTGTATCTCC TAACAGGCCT AGAACTCTAT CAAAAAGGAA AGTACCAAGA GGCACTTTCC 
TACCTGGTAT ATGCCTACCA GAGCAATGCT GCCCTGCTGA TGAAGGGGCC CCGCCGGGGG 
GTCAAAGAAT CCGTGATTGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA 
GCAGCTTCTC TTTTTGAAAC AAATGATGAT CACTCCGTAA CTGAGGGCAT TAATGTGATG 
AATGAACTGA TCATCCCCTG CATTCACCTT ATCATTAATA ATGACATTTC CAAGGATGAT 
CTGGATGCCA TTGAGGTCAT GAGAAACCAT TGGTGCTCTT ACCTTGGGCA AGATATTGCA 
GAAAATCTGC AGCTGTGCCT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA 
ATCATCGTCT TGAAAGAGCC TCCAACTATT CGACCCAATT CTCCCTATGA CCTATGTAGC 
CGATTTGCAG CTGTCATGGA GTCAATTCAG GGAGTTTCAA CTGTGACAGT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333183.1
CDS	72..3308
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346254.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346254.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGGTAT GTGAAATATG GCTCAGGCCC AGCTCGGTTC 
CCGCTCCCGG ACATGCTGAA ATATGTTATT GAATTTGCTA GTACAAAACC TGCCTCAGAA 
AGCTGTCCAC CTGAAAGTGA CACACATATG ACATTACCAC TTTCTTCAGT GCACTGCTCG 
GTTTCTGACC AGACATCCAA GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC 
CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTGATT 
AAGGCATTCC ATGAAGAATA CTCCAGGCTC TATCAGCTTG CCAAAGAGAC CCCCACCTCT 
CACAGTGATC CTCGACTTCA GCATGTCCTT GTCTACTTTT TCCAAAATGA AGCACCCAAA 
AGGGTAGTAG AACGAACCCT TCTGGAACAG TTTGCAGATA AAAATCTTAG CTATGATGAA 
AGATCAATCA GCATTATGAA GGTGGCTCAA GCGAAACTGA AGGAAATTGG TCCAGATGAC 
ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTATA GTTTGTTCCG AAAAGTGTCT 
GTGTATCTCC TAACAGGCCT AGAACTCTAT CAAAAAGGAA AGTACCAAGA GGCACTTTCC 
TACCTGGTAT ATGCCTACCA GAGCAATGCT GCCCTGCTGA TGAAGGGGCC CCGCCGGGGG 
GTCAAAGAAT CCGTGATTGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA 
GCAGCTTCTC TTTTTGAAAC AAATGATGAT CACTCCGTAA CTGAGGGCAT TAATGTGATG 
AATGAACTGA TCATCCCCTG CATTCACCTT ATCATTAATA ATGACATTTC CAAGGATGAT 
CTGGATGCCA TTGAGGTCAT GAGAAACCAT TGGTGCTCTT ACCTTGGGCA AGATATTGCA 
GAAAATCTGC AGCTGTGCCT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA 
ATCATCGTCT TGAAAGAGCC TCCAACTATT CGACCCAATT CTCCCTATGA CCTATGTAGC 
CGATTTGCAG CTGTCATGGA GTCAATTCAG GGAGTTTCAA CTGTGACAGT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346254.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333183.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu45849
Clone ID Related Accession (Same CDS sequence)	NM_001346254.2 , NM_001346254.1
Accession Version	NM_001346254.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_005271637.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.198394.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGGTAT GTGAAATATG GCTCAGGCCC AGCTCGGTTC 
CCGCTCCCGG ACATGCTGAA ATATGTTATT GAATTTGCTA GTACAAAACC TGCCTCAGAA 
AGCTGTCCAC CTGAAAGTGA CACACATATG ACATTACCAC TTTCTTCAGT GCACTGCTCG 
GTTTCTGACC AGACATCCAA GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC 
CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTGATT 
AAGGCATTCC ATGAAGAATA CTCCAGGCTC TATCAGCTTG CCAAAGAGAC CCCCACCTCT 
CACAGTGATC CTCGACTTCA GCATGTCCTT GTCTACTTTT TCCAAAATGA AGCACCCAAA 
AGGGTAGTAG AACGAACCCT TCTGGAACAG TTTGCAGATA AAAATCTTAG CTATGATGAA 
AGATCAATCA GCATTATGAA GGTGGCTCAA GCGAAACTGA AGGAAATTGG TCCAGATGAC 
ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTATA GTTTGTTCCG AAAAGTGTCT 
GTGTATCTCC TAACAGGCCT AGAACTCTAT CAAAAAGGAA AGTACCAAGA GGCACTTTCC 
TACCTGGTAT ATGCCTACCA GAGCAATGCT GCCCTGCTGA TGAAGGGGCC CCGCCGGGGG 
GTCAAAGAAT CCGTGATTGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA 
GCAGCTTCTC TTTTTGAAAC AAATGATGAT CACTCCGTAA CTGAGGGCAT TAATGTGATG 
AATGAACTGA TCATCCCCTG CATTCACCTT ATCATTAATA ATGACATTTC CAAGGATGAT 
CTGGATGCCA TTGAGGTCAT GAGAAACCAT TGGTGCTCTT ACCTTGGGCA AGATATTGCA 
GAAAATCTGC AGCTGTGCCT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA 
ATCATCGTCT TGAAAGAGCC TCCAACTATT CGACCCAATT CTCCCTATGA CCTATGTAGC 
CGATTTGCAG CTGTCATGGA GTCAATTCAG GGAGTTTCAA CTGTGACAGT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333183.1
CDS	70..3306
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346254.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346254.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGGTAT GTGAAATATG GCTCAGGCCC AGCTCGGTTC 
CCGCTCCCGG ACATGCTGAA ATATGTTATT GAATTTGCTA GTACAAAACC TGCCTCAGAA 
AGCTGTCCAC CTGAAAGTGA CACACATATG ACATTACCAC TTTCTTCAGT GCACTGCTCG 
GTTTCTGACC AGACATCCAA GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC 
CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTGATT 
AAGGCATTCC ATGAAGAATA CTCCAGGCTC TATCAGCTTG CCAAAGAGAC CCCCACCTCT 
CACAGTGATC CTCGACTTCA GCATGTCCTT GTCTACTTTT TCCAAAATGA AGCACCCAAA 
AGGGTAGTAG AACGAACCCT TCTGGAACAG TTTGCAGATA AAAATCTTAG CTATGATGAA 
AGATCAATCA GCATTATGAA GGTGGCTCAA GCGAAACTGA AGGAAATTGG TCCAGATGAC 
ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTATA GTTTGTTCCG AAAAGTGTCT 
GTGTATCTCC TAACAGGCCT AGAACTCTAT CAAAAAGGAA AGTACCAAGA GGCACTTTCC 
TACCTGGTAT ATGCCTACCA GAGCAATGCT GCCCTGCTGA TGAAGGGGCC CCGCCGGGGG 
GTCAAAGAAT CCGTGATTGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA 
GCAGCTTCTC TTTTTGAAAC AAATGATGAT CACTCCGTAA CTGAGGGCAT TAATGTGATG 
AATGAACTGA TCATCCCCTG CATTCACCTT ATCATTAATA ATGACATTTC CAAGGATGAT 
CTGGATGCCA TTGAGGTCAT GAGAAACCAT TGGTGCTCTT ACCTTGGGCA AGATATTGCA 
GAAAATCTGC AGCTGTGCCT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA 
ATCATCGTCT TGAAAGAGCC TCCAACTATT CGACCCAATT CTCCCTATGA CCTATGTAGC 
CGATTTGCAG CTGTCATGGA GTCAATTCAG GGAGTTTCAA CTGTGACAGT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346258.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333187.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu45851
Clone ID Related Accession (Same CDS sequence)	NM_001346258.1 , NM_001346258.2
Accession Version	NM_001346258.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Oct 4, 2016 this sequence version replaced XM_005271639.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.37634.1, SRR1803612.80824.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333187.1
CDS	70..3207
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346258.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346258.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001346258.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333187.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 8, mRNA.

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CloneID	OHu45851
Clone ID Related Accession (Same CDS sequence)	NM_001346258.1 , NM_001346258.2
Accession Version	NM_001346258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001874.4, AP003170.4 and KF459544.1. On Jun 2, 2019 this sequence version replaced NM_001346258.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.37634.1, SRR1803612.80824.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333187.1
CDS	72..3209
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001346258.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346258.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGTCCC 
AGGAACAAAT CTGAAAATCC AATGGTGCAG CTGTTCTATG GTACTTTCCT GACTGAAGGG 
GTTCGTGAAG GAAAACCCTT TTGTAACAAT GAGACCTTCG GCCAGTATCC TCTTCAGGTA 
AACGGTTATC GCAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAGGG TGATGTTGAG 
CTTCTTCCCT CCGATCACTC GGTGAAGTAT GGACAAGAGC GTTGGTTTAC AAAGCTACCT 
CCAGTGTTGA CCTTTGAACT CTCAAGATTT GAGTTTAATC AGTCCCTTGG GCAGCCAGAG 
AAAATTCACA ATAAGCTGGA ATTTCCTCAG ATTATTTATA TGGACAGGTA CATGTACAGG 
AGCAAGGAGC TTATTCGAAA TAAGAGAGAG TGTATTCGAA AGTTGAAGGA GGAAATAAAA 
ATTCTGCAGC AAAAATTGGA AAGGTATGTG AAATATGGCT CAGGCCCAGC TCGGTTCCCG 
CTCCCGGACA TGCTGAAATA TGTTATTGAA TTTGCTAGTA CAAAACCTGC CTCAGAAAGC 
TGTCCACCTG AAAGTGACAC ACATATGACA TTACCACTTT CTTCAGTGCA CTGCTCGGTT 
TCTGACCAGA CATCCAAGGA AAGTACAAGT ACAGAAAGCT CTTCTCAGGA TGTTGAAAGT 
ACCTTTTCTT CTCCTGAAGA TTCTTTACCC AAGTCTAAAC CACTGACATC TTCTCGGTCT 
TCCATGGAAA TGCCTTCACA GCCAGCTCCA CGAACAGTCA CAGATGAGGA GATAAATTTT 
GTTAAGACCT GTCTTCAGAG ATGGAGGAGT GAGATTGAAC AAGATATACA AGATTTAAAG 
ACTTGTATTG CAAGTACTAC TCAGACTATT GAACAGATGT ACTGCGATCC TCTCCTTCGT 
CAGGTGCCTT ATCGCTTGCA TGCAGTTCTT GTTCATGAAG GACAAGCAAA TGCTGGACAC 
TATTGGGCCT ATATCTATAA TCAACCCCGA CAGAGCTGGC TCAAGTACAA TGACATCTCT 
GTTACTGAAT CTTCCTGGGA AGAAGTTGAA AGAGATTCCT ATGGAGGCCT GAGAAATGTT 
AGTGCTTACT GTCTGATGTA CATTAATGAC AAACTACCCT ACTTCAATGC AGAGGCAGCC 
CCAACTGAAT CAGATCAAAT GTCAGAAGTG GAAGCCCTAT CTGTGGAACT CAAGCATTAC 
ATTCAGGAGG ATAACTGGCG GTTTGAGCAG GAAGTAGAGG AGTGGGAAGA AGAGCAGTCT 
TGCAAAATCC CTCAAATGGA GTCCTCCACC AACTCCTCAT CACAGGACTA CTCTACATCA 
CAAGAGCCTT CAGTAGCCTC TTCTCATGGG GTTCGCTGCT TGTCGTCTGA GCATGCTGTG 
ATTGTAAAGG AGCAAACTGC CCAGGCTATT GCAAACACAG CCCGTGCCTA TGAGAAGAGC 
GGTGTAGAAG CGGCACTGAG TGAGGCATTC CATGAAGAAT ACTCCAGGCT CTATCAGCTT 
GCCAAAGAGA CCCCCACCTC TCACAGTGAT CCTCGACTTC AGCATGTCCT TGTCTACTTT 
TTCCAAAATG AAGCACCCAA AAGGGTAGTA GAACGAACCC TTCTGGAACA GTTTGCAGAT 
AAAAATCTTA GCTATGATGA AAGATCAATC AGCATTATGA AGGTGGCTCA AGCGAAACTG 
AAGGAAATTG GTCCAGATGA CATGAATATG GAAGAGTACA AGAAGTGGCA TGAAGATTAT 
AGTTTGTTCC GAAAAGTGTC TGTGTATCTC CTAACAGGCC TAGAACTCTA TCAAAAAGGA 
AAGTACCAAG AGGCACTTTC CTACCTGGTA TATGCCTACC AGAGCAATGC TGCCCTGCTG 
ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA TCCGTGATTG CTTTATACCG AAGAAAATGC 
CTTCTGGAGC TGAATGCCAA AGCAGCTTCT CTTTTTGAAA CAAATGATGA TCACTCCGTA 
ACTGAGGGCA TTAATGTGAT GAATGAACTG ATCATCCCCT GCATTCACCT TATCATTAAT 
AATGACATTT CCAAGGATGA TCTGGATGCC ATTGAGGTCA TGAGAAACCA TTGGTGCTCT 
TACCTTGGGC AAGATATTGC AGAAAATCTG CAGCTGTGCC TAGGGGAGTT TCTACCCAGA 
CTTCTAGATC CTTCTGCAGA AATCATCGTC TTGAAAGAGC CTCCAACTAT TCGACCCAAT 
TCTCCCTATG ACCTATGTAG CCGATTTGCA GCTGTCATGG AGTCAATTCA GGGAGTTTCA 
ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005271630.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271687.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu45844
Clone ID Related Accession (Same CDS sequence)	XM_005271630.2
Accession Version	XM_005271630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005271630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGGTAT GTGAAATATG GCTCAGGCCC AGCTCGGTTC 
CCGCTCCCGG ACATGCTGAA ATATGTTATT GAATTTGCTA GTACAAAACC TGCCTCAGAA 
AGCTGTCCAC CTGAAAGTGA CACACATATG ACATTACCAC TTTCTTCAGT GCACTGCTCG 
GTTTCTGACC AGACATCCAA GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGCTT AAAGAAGCTG AACCCAAGAA GCCCATGCCC 
CAGGAAACAA ACCTTGCAGA GCAGTCAGAA CAGCCCCCAA AGGCTAATGA TGCAGAGTCT 
ACTGCCCAGC CTAATTCTGA GGTCTCTGAA GTCGAGATTC CCAGTGTGGG AAGGATTCTG 
GTTAGATCTG ATGCAGATGG ATATGATGAG GAGGTGATGC TGAGCCCTGC CATGCAAGGG 
GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG 
CTGATTAAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271687.1
CDS	66..3488
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_005271630.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271630.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGGTAT GTGAAATATG GCTCAGGCCC AGCTCGGTTC 
CCGCTCCCGG ACATGCTGAA ATATGTTATT GAATTTGCTA GTACAAAACC TGCCTCAGAA 
AGCTGTCCAC CTGAAAGTGA CACACATATG ACATTACCAC TTTCTTCAGT GCACTGCTCG 
GTTTCTGACC AGACATCCAA GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGCTT AAAGAAGCTG AACCCAAGAA GCCCATGCCC 
CAGGAAACAA ACCTTGCAGA GCAGTCAGAA CAGCCCCCAA AGGCTAATGA TGCAGAGTCT 
ACTGCCCAGC CTAATTCTGA GGTCTCTGAA GTCGAGATTC CCAGTGTGGG AAGGATTCTG 
GTTAGATCTG ATGCAGATGG ATATGATGAG GAGGTGATGC TGAGCCCTGC CATGCAAGGG 
GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG 
CTGATTAAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005271632.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271689.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

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CloneID	OHu45846
Clone ID Related Accession (Same CDS sequence)	XM_005271632.2
Accession Version	XM_005271632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005271632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAACT ATATTTGTCG TACAAAGCTG GCAGACAGAT ACCCTCAGAG CTGCCAAATG 
CTGTTAAATC AACTGAGAGA AATCACAGGC ATTCAGGACC CTTCCTTTCT CCATGAAGCT 
CTGAAGGCCA GTAATGGTGA CATTACTCAG GCAGTCAGCC TTCTCACTGA TGAGAGAGTT 
AAGGAGCCCA GTCAAGACAC TGTTGCTACA GAACCATCTG AAGTAGAGGG GAGTGCTGCC 
AACAAGGAAG TATTAGCAAA AGTTATAGAC CTTACTCATG ATAACAAAGA TGATCTTCAG 
GCTGCCATTG CTTTGAGTCT ACTGGAGTCT CCCAAAATTC AAGCTGATGG AAGAGATCTT 
AACAGGATGC ATGAAGCAAC CTCTGCAGAA ACTAAACGCT CAAAGAGAAA ACGCTGTGAA 
GTCTGGGGAG AAAACCCCAA TCCCAATGAC TGGAGGAGAG TTGATGGTTG GCCAGTTGGG 
CTGAAAAATG TTGGCAATAC ATGTTGGTTT AGTGCTGTTA TTCAGTCTCT CTTTCAATTG 
CCTGAATTTC GAAGACTTGT TCTCAGTTAT AGTCTGCCAC AAAATGTACT TGAAAATTGT 
CGAAGTCATA CAGAAAAGAG AAATATCATG TTTATGCAAG AGCTTCAGTA TTTGTTTGCT 
CTAATGATGG GATCAAATAG AAAATTTGTA GACCCGTCTG CAGCCCTGGA TCTATTAAAG 
GGAGCATTCC GATCATCTGA GGAACAGCAG CAAGATGTGA GTGAATTCAC ACACAAGCTC 
CTGGATTGGC TAGAGGACGC ATTCCAGCTA GCTGTTAATG TTAACAGCAG TCCCAGGAAC 
AAATCTGAAA ATCCAATGGT GCAGCTGTTC TATGGTACTT TCCTGACTGA AGGGGTTCGT 
GAAGGAAAAC CCTTTTGTAA CAATGAGACC TTCGGCCAGT ATCCTCTTCA GGTAAACGGT 
TATCGCAACT TAGACGAGTG TTTGGAAGGG GCCATGGTGG AGGGTGATGT TGAGCTTCTT 
CCCTCCGATC ACTCGGTGAA GTATGGACAA GAGCGTTGGT TTACAAAGCT ACCTCCAGTG 
TTGACCTTTG AACTCTCAAG ATTTGAGTTT AATCAGTCCC TTGGGCAGCC AGAGAAAATT 
CACAATAAGC TGGAATTTCC TCAGATTATT TATATGGACA GGTACATGTA CAGGAGCAAG 
GAGCTTATTC GAAATAAGAG AGAGTGTATT CGAAAGTTGA AGGAGGAAAT AAAAATTCTG 
CAGCAAAAAT TGGAAAGGTA TGTGAAATAT GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG 
GACATGCTGA AATATGTTAT TGAATTTGCT AGTACAAAAC CTGCCTCAGA AAGCTGTCCA 
CCTGAAAGTG ACACACATAT GACATTACCA CTTTCTTCAG TGCACTGCTC GGTTTCTGAC 
CAGACATCCA AGGAAAGTAC AAGTACAGAA AGCTCTTCTC AGGATGTTGA AAGTACCTTT 
TCTTCTCCTG AAGATTCTTT ACCCAAGTCT AAACCACTGA CATCTTCTCG GTCTTCCATG 
GAAATGCCTT CACAGCCAGC TCCACGAACA GTCACAGATG AGGAGATAAA TTTTGTTAAG 
ACCTGTCTTC AGAGATGGAG GAGTGAGATT GAACAAGATA TACAAGATTT AAAGACTTGT 
ATTGCAAGTA CTACTCAGAC TATTGAACAG ATGTACTGCG ATCCTCTCCT TCGTCAGGTG 
CCTTATCGCT TGCATGCAGT TCTTGTTCAT GAAGGACAAG CAAATGCTGG ACACTATTGG 
GCCTATATCT ATAATCAACC CCGACAGAGC TGGCTCAAGT ACAATGACAT CTCTGTTACT 
GAATCTTCCT GGGAAGAAGT TGAAAGAGAT TCCTATGGAG GCCTGAGAAA TGTTAGTGCT 
TACTGTCTGA TGTACATTAA TGACAAACTA CCCTACTTCA ATGCAGAGGC AGCCCCAACT 
GAATCAGATC AAATGTCAGA AGTGGAAGCC CTATCTGTGG AACTCAAGCA TTACATTCAG 
GAGGATAACT GGCGGTTTGA GCAGGAAGTA GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA 
ATCCCTCAAA TGGAGTCCTC CACCAACTCC TCATCACAGG ACTACTCTAC ATCACAAGAG 
CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC TGCTTGTCGT CTGAGCATGC TGTGATTGTA 
AAGGAGCAAA CTGCCCAGGC TATTGCAAAC ACAGCCCGTG CCTATGAGAA GAGCGGTGTA 
GAAGCGGCAC TGAGTGAGCT TAAAGAAGCT GAACCCAAGA AGCCCATGCC CCAGGAAACA 
AACCTTGCAG AGCAGTCAGA ACAGCCCCCA AAGGCTAATG ATGCAGAGTC TACTGCCCAG 
CCTAATTCTG AGGTCTCTGA AGTCGAGATT CCCAGTGTGG GAAGGATTCT GGTTAGATCT 
GATGCAGATG GATATGATGA GGAGGTGATG CTGAGCCCTG CCATGCAAGG GGTCATCCTG 
GCCATAGCTA AAGCCCGTCA GACCTTTGAC CGAGATGGGT CTGAAGCAGG GCTGATTAAG 
GCATTCCATG AAGAATACTC CAGGCTCTAT CAGCTTGCCA AAGAGACCCC CACCTCTCAC 
AGTGATCCTC GACTTCAGCA TGTCCTTGTC TACTTTTTCC AAAATGAAGC ACCCAAAAGG 
GTAGTAGAAC GAACCCTTCT GGAACAGTTT GCAGATAAAA ATCTTAGCTA TGATGAAAGA 
TCAATCAGCA TTATGAAGGT GGCTCAAGCG AAACTGAAGG AAATTGGTCC AGATGACATG 
AATATGGAAG AGTACAAGAA GTGGCATGAA GATTATAGTT TGTTCCGAAA AGTGTCTGTG 
TATCTCCTAA CAGGCCTAGA ACTCTATCAA AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC 
CTGGTATATG CCTACCAGAG CAATGCTGCC CTGCTGATGA AGGGGCCCCG CCGGGGGGTC 
AAAGAATCCG TGATTGCTTT ATACCGAAGA AAATGCCTTC TGGAGCTGAA TGCCAAAGCA 
GCTTCTCTTT TTGAAACAAA TGATGATCAC TCCGTAACTG AGGGCATTAA TGTGATGAAT 
GAACTGATCA TCCCCTGCAT TCACCTTATC ATTAATAATG ACATTTCCAA GGATGATCTG 
GATGCCATTG AGGTCATGAG AAACCATTGG TGCTCTTACC TTGGGCAAGA TATTGCAGAA 
AATCTGCAGC TGTGCCTAGG GGAGTTTCTA CCCAGACTTC TAGATCCTTC TGCAGAAATC 
ATCGTCTTGA AAGAGCCTCC AACTATTCGA CCCAATTCTC CCTATGACCT ATGTAGCCGA 
TTTGCAGCTG TCATGGAGTC AATTCAGGGA GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271689.1
CDS	405..3818
Translation	MRNYICRTKLADRYPQSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_005271632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271632.2

ORF Insert Sequence:

ATGAGAAACT ATATTTGTCG TACAAAGCTG GCAGACAGAT ACCCTCAGAG CTGCCAAATG 
CTGTTAAATC AACTGAGAGA AATCACAGGC ATTCAGGACC CTTCCTTTCT CCATGAAGCT 
CTGAAGGCCA GTAATGGTGA CATTACTCAG GCAGTCAGCC TTCTCACTGA TGAGAGAGTT 
AAGGAGCCCA GTCAAGACAC TGTTGCTACA GAACCATCTG AAGTAGAGGG GAGTGCTGCC 
AACAAGGAAG TATTAGCAAA AGTTATAGAC CTTACTCATG ATAACAAAGA TGATCTTCAG 
GCTGCCATTG CTTTGAGTCT ACTGGAGTCT CCCAAAATTC AAGCTGATGG AAGAGATCTT 
AACAGGATGC ATGAAGCAAC CTCTGCAGAA ACTAAACGCT CAAAGAGAAA ACGCTGTGAA 
GTCTGGGGAG AAAACCCCAA TCCCAATGAC TGGAGGAGAG TTGATGGTTG GCCAGTTGGG 
CTGAAAAATG TTGGCAATAC ATGTTGGTTT AGTGCTGTTA TTCAGTCTCT CTTTCAATTG 
CCTGAATTTC GAAGACTTGT TCTCAGTTAT AGTCTGCCAC AAAATGTACT TGAAAATTGT 
CGAAGTCATA CAGAAAAGAG AAATATCATG TTTATGCAAG AGCTTCAGTA TTTGTTTGCT 
CTAATGATGG GATCAAATAG AAAATTTGTA GACCCGTCTG CAGCCCTGGA TCTATTAAAG 
GGAGCATTCC GATCATCTGA GGAACAGCAG CAAGATGTGA GTGAATTCAC ACACAAGCTC 
CTGGATTGGC TAGAGGACGC ATTCCAGCTA GCTGTTAATG TTAACAGCAG TCCCAGGAAC 
AAATCTGAAA ATCCAATGGT GCAGCTGTTC TATGGTACTT TCCTGACTGA AGGGGTTCGT 
GAAGGAAAAC CCTTTTGTAA CAATGAGACC TTCGGCCAGT ATCCTCTTCA GGTAAACGGT 
TATCGCAACT TAGACGAGTG TTTGGAAGGG GCCATGGTGG AGGGTGATGT TGAGCTTCTT 
CCCTCCGATC ACTCGGTGAA GTATGGACAA GAGCGTTGGT TTACAAAGCT ACCTCCAGTG 
TTGACCTTTG AACTCTCAAG ATTTGAGTTT AATCAGTCCC TTGGGCAGCC AGAGAAAATT 
CACAATAAGC TGGAATTTCC TCAGATTATT TATATGGACA GGTACATGTA CAGGAGCAAG 
GAGCTTATTC GAAATAAGAG AGAGTGTATT CGAAAGTTGA AGGAGGAAAT AAAAATTCTG 
CAGCAAAAAT TGGAAAGGTA TGTGAAATAT GGCTCAGGCC CAGCTCGGTT CCCGCTCCCG 
GACATGCTGA AATATGTTAT TGAATTTGCT AGTACAAAAC CTGCCTCAGA AAGCTGTCCA 
CCTGAAAGTG ACACACATAT GACATTACCA CTTTCTTCAG TGCACTGCTC GGTTTCTGAC 
CAGACATCCA AGGAAAGTAC AAGTACAGAA AGCTCTTCTC AGGATGTTGA AAGTACCTTT 
TCTTCTCCTG AAGATTCTTT ACCCAAGTCT AAACCACTGA CATCTTCTCG GTCTTCCATG 
GAAATGCCTT CACAGCCAGC TCCACGAACA GTCACAGATG AGGAGATAAA TTTTGTTAAG 
ACCTGTCTTC AGAGATGGAG GAGTGAGATT GAACAAGATA TACAAGATTT AAAGACTTGT 
ATTGCAAGTA CTACTCAGAC TATTGAACAG ATGTACTGCG ATCCTCTCCT TCGTCAGGTG 
CCTTATCGCT TGCATGCAGT TCTTGTTCAT GAAGGACAAG CAAATGCTGG ACACTATTGG 
GCCTATATCT ATAATCAACC CCGACAGAGC TGGCTCAAGT ACAATGACAT CTCTGTTACT 
GAATCTTCCT GGGAAGAAGT TGAAAGAGAT TCCTATGGAG GCCTGAGAAA TGTTAGTGCT 
TACTGTCTGA TGTACATTAA TGACAAACTA CCCTACTTCA ATGCAGAGGC AGCCCCAACT 
GAATCAGATC AAATGTCAGA AGTGGAAGCC CTATCTGTGG AACTCAAGCA TTACATTCAG 
GAGGATAACT GGCGGTTTGA GCAGGAAGTA GAGGAGTGGG AAGAAGAGCA GTCTTGCAAA 
ATCCCTCAAA TGGAGTCCTC CACCAACTCC TCATCACAGG ACTACTCTAC ATCACAAGAG 
CCTTCAGTAG CCTCTTCTCA TGGGGTTCGC TGCTTGTCGT CTGAGCATGC TGTGATTGTA 
AAGGAGCAAA CTGCCCAGGC TATTGCAAAC ACAGCCCGTG CCTATGAGAA GAGCGGTGTA 
GAAGCGGCAC TGAGTGAGCT TAAAGAAGCT GAACCCAAGA AGCCCATGCC CCAGGAAACA 
AACCTTGCAG AGCAGTCAGA ACAGCCCCCA AAGGCTAATG ATGCAGAGTC TACTGCCCAG 
CCTAATTCTG AGGTCTCTGA AGTCGAGATT CCCAGTGTGG GAAGGATTCT GGTTAGATCT 
GATGCAGATG GATATGATGA GGAGGTGATG CTGAGCCCTG CCATGCAAGG GGTCATCCTG 
GCCATAGCTA AAGCCCGTCA GACCTTTGAC CGAGATGGGT CTGAAGCAGG GCTGATTAAG 
GCATTCCATG AAGAATACTC CAGGCTCTAT CAGCTTGCCA AAGAGACCCC CACCTCTCAC 
AGTGATCCTC GACTTCAGCA TGTCCTTGTC TACTTTTTCC AAAATGAAGC ACCCAAAAGG 
GTAGTAGAAC GAACCCTTCT GGAACAGTTT GCAGATAAAA ATCTTAGCTA TGATGAAAGA 
TCAATCAGCA TTATGAAGGT GGCTCAAGCG AAACTGAAGG AAATTGGTCC AGATGACATG 
AATATGGAAG AGTACAAGAA GTGGCATGAA GATTATAGTT TGTTCCGAAA AGTGTCTGTG 
TATCTCCTAA CAGGCCTAGA ACTCTATCAA AAAGGAAAGT ACCAAGAGGC ACTTTCCTAC 
CTGGTATATG CCTACCAGAG CAATGCTGCC CTGCTGATGA AGGGGCCCCG CCGGGGGGTC 
AAAGAATCCG TGATTGCTTT ATACCGAAGA AAATGCCTTC TGGAGCTGAA TGCCAAAGCA 
GCTTCTCTTT TTGAAACAAA TGATGATCAC TCCGTAACTG AGGGCATTAA TGTGATGAAT 
GAACTGATCA TCCCCTGCAT TCACCTTATC ATTAATAATG ACATTTCCAA GGATGATCTG 
GATGCCATTG AGGTCATGAG AAACCATTGG TGCTCTTACC TTGGGCAAGA TATTGCAGAA 
AATCTGCAGC TGTGCCTAGG GGAGTTTCTA CCCAGACTTC TAGATCCTTC TGCAGAAATC 
ATCGTCTTGA AAGAGCCTCC AACTATTCGA CCCAATTCTC CCTATGACCT ATGTAGCCGA 
TTTGCAGCTG TCATGGAGTC AATTCAGGGA GTTTCAACTG TGACAGTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005271633.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271690.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

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CloneID	OHu45847
Clone ID Related Accession (Same CDS sequence)	XM_005271633.4
Accession Version	XM_005271633.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005271633.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTAA ATCAACTGAG AGAAATCACA GGCATTCAGG ACCCTTCCTT TCTCCATGAA 
GCTCTGAAGG CCAGTAATGG TGACATTACT CAGGCAGTCA GCCTTCTCAC TGATGAGAGA 
GTTAAGGAGC CCAGTCAAGA CACTGTTGCT ACAGAACCAT CTGAAGTAGA GGGGAGTGCT 
GCCAACAAGG AAGTATTAGC AAAAGTTATA GACCTTACTC ATGATAACAA AGATGATCTT 
CAGGCTGCCA TTGCTTTGAG TCTACTGGAG TCTCCCAAAA TTCAAGCTGA TGGAAGAGAT 
CTTAACAGGA TGCATGAAGC AACCTCTGCA GAAACTAAAC GCTCAAAGAG AAAACGCTGT 
GAAGTCTGGG GAGAAAACCC CAATCCCAAT GACTGGAGGA GAGTTGATGG TTGGCCAGTT 
GGGCTGAAAA ATGTTGGCAA TACATGTTGG TTTAGTGCTG TTATTCAGTC TCTCTTTCAA 
TTGCCTGAAT TTCGAAGACT TGTTCTCAGT TATAGTCTGC CACAAAATGT ACTTGAAAAT 
TGTCGAAGTC ATACAGAAAA GAGAAATATC ATGTTTATGC AAGAGCTTCA GTATTTGTTT 
GCTCTAATGA TGGGATCAAA TAGAAAATTT GTAGACCCGT CTGCAGCCCT GGATCTATTA 
AAGGGAGCAT TCCGATCATC TGAGGAACAG CAGCAAGATG TGAGTGAATT CACACACAAG 
CTCCTGGATT GGCTAGAGGA CGCATTCCAG CTAGCTGTTA ATGTTAACAG CAGTCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTGAC TGAAGGGGTT 
CGTGAAGGAA AACCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCTCT TCAGGTAAAC 
GGTTATCGCA ACTTAGACGA GTGTTTGGAA GGGGCCATGG TGGAGGGTGA TGTTGAGCTT 
CTTCCCTCCG ATCACTCGGT GAAGTATGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTTAATCAGT CCCTTGGGCA GCCAGAGAAA 
ATTCACAATA AGCTGGAATT TCCTCAGATT ATTTATATGG ACAGGTACAT GTACAGGAGC 
AAGGAGCTTA TTCGAAATAA GAGAGAGTGT ATTCGAAAGT TGAAGGAGGA AATAAAAATT 
CTGCAGCAAA AATTGGAAAG GTATGTGAAA TATGGCTCAG GCCCAGCTCG GTTCCCGCTC 
CCGGACATGC TGAAATATGT TATTGAATTT GCTAGTACAA AACCTGCCTC AGAAAGCTGT 
CCACCTGAAA GTGACACACA TATGACATTA CCACTTTCTT CAGTGCACTG CTCGGTTTCT 
GACCAGACAT CCAAGGAAAG TACAAGTACA GAAAGCTCTT CTCAGGATGT TGAAAGTACC 
TTTTCTTCTC CTGAAGATTC TTTACCCAAG TCTAAACCAC TGACATCTTC TCGGTCTTCC 
ATGGAAATGC CTTCACAGCC AGCTCCACGA ACAGTCACAG ATGAGGAGAT AAATTTTGTT 
AAGACCTGTC TTCAGAGATG GAGGAGTGAG ATTGAACAAG ATATACAAGA TTTAAAGACT 
TGTATTGCAA GTACTACTCA GACTATTGAA CAGATGTACT GCGATCCTCT CCTTCGTCAG 
GTGCCTTATC GCTTGCATGC AGTTCTTGTT CATGAAGGAC AAGCAAATGC TGGACACTAT 
TGGGCCTATA TCTATAATCA ACCCCGACAG AGCTGGCTCA AGTACAATGA CATCTCTGTT 
ACTGAATCTT CCTGGGAAGA AGTTGAAAGA GATTCCTATG GAGGCCTGAG AAATGTTAGT 
GCTTACTGTC TGATGTACAT TAATGACAAA CTACCCTACT TCAATGCAGA GGCAGCCCCA 
ACTGAATCAG ATCAAATGTC AGAAGTGGAA GCCCTATCTG TGGAACTCAA GCATTACATT 
CAGGAGGATA ACTGGCGGTT TGAGCAGGAA GTAGAGGAGT GGGAAGAAGA GCAGTCTTGC 
AAAATCCCTC AAATGGAGTC CTCCACCAAC TCCTCATCAC AGGACTACTC TACATCACAA 
GAGCCTTCAG TAGCCTCTTC TCATGGGGTT CGCTGCTTGT CGTCTGAGCA TGCTGTGATT 
GTAAAGGAGC AAACTGCCCA GGCTATTGCA AACACAGCCC GTGCCTATGA GAAGAGCGGT 
GTAGAAGCGG CACTGAGTGA GCTTAAAGAA GCTGAACCCA AGAAGCCCAT GCCCCAGGAA 
ACAAACCTTG CAGAGCAGTC AGAACAGCCC CCAAAGGCTA ATGATGCAGA GTCTACTGCC 
CAGCCTAATT CTGAGGTCTC TGAAGTCGAG ATTCCCAGTG TGGGAAGGAT TCTGGTTAGA 
TCTGATGCAG ATGGATATGA TGAGGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC 
CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTGATT 
AAGGCATTCC ATGAAGAATA CTCCAGGCTC TATCAGCTTG CCAAAGAGAC CCCCACCTCT 
CACAGTGATC CTCGACTTCA GCATGTCCTT GTCTACTTTT TCCAAAATGA AGCACCCAAA 
AGGGTAGTAG AACGAACCCT TCTGGAACAG TTTGCAGATA AAAATCTTAG CTATGATGAA 
AGATCAATCA GCATTATGAA GGTGGCTCAA GCGAAACTGA AGGAAATTGG TCCAGATGAC 
ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTATA GTTTGTTCCG AAAAGTGTCT 
GTGTATCTCC TAACAGGCCT AGAACTCTAT CAAAAAGGAA AGTACCAAGA GGCACTTTCC 
TACCTGGTAT ATGCCTACCA GAGCAATGCT GCCCTGCTGA TGAAGGGGCC CCGCCGGGGG 
GTCAAAGAAT CCGTGATTGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA 
GCAGCTTCTC TTTTTGAAAC AAATGATGAT CACTCCGTAA CTGAGGGCAT TAATGTGATG 
AATGAACTGA TCATCCCCTG CATTCACCTT ATCATTAATA ATGACATTTC CAAGGATGAT 
CTGGATGCCA TTGAGGTCAT GAGAAACCAT TGGTGCTCTT ACCTTGGGCA AGATATTGCA 
GAAAATCTGC AGCTGTGCCT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA 
ATCATCGTCT TGAAAGAGCC TCCAACTATT CGACCCAATT CTCCCTATGA CCTATGTAGC 
CGATTTGCAG CTGTCATGGA GTCAATTCAG GGAGTTTCAA CTGTGACAGT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271690.1
CDS	190..3546
Translation	MLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_005271633.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271633.4

ORF Insert Sequence:

ATGCTGTTAA ATCAACTGAG AGAAATCACA GGCATTCAGG ACCCTTCCTT TCTCCATGAA 
GCTCTGAAGG CCAGTAATGG TGACATTACT CAGGCAGTCA GCCTTCTCAC TGATGAGAGA 
GTTAAGGAGC CCAGTCAAGA CACTGTTGCT ACAGAACCAT CTGAAGTAGA GGGGAGTGCT 
GCCAACAAGG AAGTATTAGC AAAAGTTATA GACCTTACTC ATGATAACAA AGATGATCTT 
CAGGCTGCCA TTGCTTTGAG TCTACTGGAG TCTCCCAAAA TTCAAGCTGA TGGAAGAGAT 
CTTAACAGGA TGCATGAAGC AACCTCTGCA GAAACTAAAC GCTCAAAGAG AAAACGCTGT 
GAAGTCTGGG GAGAAAACCC CAATCCCAAT GACTGGAGGA GAGTTGATGG TTGGCCAGTT 
GGGCTGAAAA ATGTTGGCAA TACATGTTGG TTTAGTGCTG TTATTCAGTC TCTCTTTCAA 
TTGCCTGAAT TTCGAAGACT TGTTCTCAGT TATAGTCTGC CACAAAATGT ACTTGAAAAT 
TGTCGAAGTC ATACAGAAAA GAGAAATATC ATGTTTATGC AAGAGCTTCA GTATTTGTTT 
GCTCTAATGA TGGGATCAAA TAGAAAATTT GTAGACCCGT CTGCAGCCCT GGATCTATTA 
AAGGGAGCAT TCCGATCATC TGAGGAACAG CAGCAAGATG TGAGTGAATT CACACACAAG 
CTCCTGGATT GGCTAGAGGA CGCATTCCAG CTAGCTGTTA ATGTTAACAG CAGTCCCAGG 
AACAAATCTG AAAATCCAAT GGTGCAGCTG TTCTATGGTA CTTTCCTGAC TGAAGGGGTT 
CGTGAAGGAA AACCCTTTTG TAACAATGAG ACCTTCGGCC AGTATCCTCT TCAGGTAAAC 
GGTTATCGCA ACTTAGACGA GTGTTTGGAA GGGGCCATGG TGGAGGGTGA TGTTGAGCTT 
CTTCCCTCCG ATCACTCGGT GAAGTATGGA CAAGAGCGTT GGTTTACAAA GCTACCTCCA 
GTGTTGACCT TTGAACTCTC AAGATTTGAG TTTAATCAGT CCCTTGGGCA GCCAGAGAAA 
ATTCACAATA AGCTGGAATT TCCTCAGATT ATTTATATGG ACAGGTACAT GTACAGGAGC 
AAGGAGCTTA TTCGAAATAA GAGAGAGTGT ATTCGAAAGT TGAAGGAGGA AATAAAAATT 
CTGCAGCAAA AATTGGAAAG GTATGTGAAA TATGGCTCAG GCCCAGCTCG GTTCCCGCTC 
CCGGACATGC TGAAATATGT TATTGAATTT GCTAGTACAA AACCTGCCTC AGAAAGCTGT 
CCACCTGAAA GTGACACACA TATGACATTA CCACTTTCTT CAGTGCACTG CTCGGTTTCT 
GACCAGACAT CCAAGGAAAG TACAAGTACA GAAAGCTCTT CTCAGGATGT TGAAAGTACC 
TTTTCTTCTC CTGAAGATTC TTTACCCAAG TCTAAACCAC TGACATCTTC TCGGTCTTCC 
ATGGAAATGC CTTCACAGCC AGCTCCACGA ACAGTCACAG ATGAGGAGAT AAATTTTGTT 
AAGACCTGTC TTCAGAGATG GAGGAGTGAG ATTGAACAAG ATATACAAGA TTTAAAGACT 
TGTATTGCAA GTACTACTCA GACTATTGAA CAGATGTACT GCGATCCTCT CCTTCGTCAG 
GTGCCTTATC GCTTGCATGC AGTTCTTGTT CATGAAGGAC AAGCAAATGC TGGACACTAT 
TGGGCCTATA TCTATAATCA ACCCCGACAG AGCTGGCTCA AGTACAATGA CATCTCTGTT 
ACTGAATCTT CCTGGGAAGA AGTTGAAAGA GATTCCTATG GAGGCCTGAG AAATGTTAGT 
GCTTACTGTC TGATGTACAT TAATGACAAA CTACCCTACT TCAATGCAGA GGCAGCCCCA 
ACTGAATCAG ATCAAATGTC AGAAGTGGAA GCCCTATCTG TGGAACTCAA GCATTACATT 
CAGGAGGATA ACTGGCGGTT TGAGCAGGAA GTAGAGGAGT GGGAAGAAGA GCAGTCTTGC 
AAAATCCCTC AAATGGAGTC CTCCACCAAC TCCTCATCAC AGGACTACTC TACATCACAA 
GAGCCTTCAG TAGCCTCTTC TCATGGGGTT CGCTGCTTGT CGTCTGAGCA TGCTGTGATT 
GTAAAGGAGC AAACTGCCCA GGCTATTGCA AACACAGCCC GTGCCTATGA GAAGAGCGGT 
GTAGAAGCGG CACTGAGTGA GCTTAAAGAA GCTGAACCCA AGAAGCCCAT GCCCCAGGAA 
ACAAACCTTG CAGAGCAGTC AGAACAGCCC CCAAAGGCTA ATGATGCAGA GTCTACTGCC 
CAGCCTAATT CTGAGGTCTC TGAAGTCGAG ATTCCCAGTG TGGGAAGGAT TCTGGTTAGA 
TCTGATGCAG ATGGATATGA TGAGGAGGTG ATGCTGAGCC CTGCCATGCA AGGGGTCATC 
CTGGCCATAG CTAAAGCCCG TCAGACCTTT GACCGAGATG GGTCTGAAGC AGGGCTGATT 
AAGGCATTCC ATGAAGAATA CTCCAGGCTC TATCAGCTTG CCAAAGAGAC CCCCACCTCT 
CACAGTGATC CTCGACTTCA GCATGTCCTT GTCTACTTTT TCCAAAATGA AGCACCCAAA 
AGGGTAGTAG AACGAACCCT TCTGGAACAG TTTGCAGATA AAAATCTTAG CTATGATGAA 
AGATCAATCA GCATTATGAA GGTGGCTCAA GCGAAACTGA AGGAAATTGG TCCAGATGAC 
ATGAATATGG AAGAGTACAA GAAGTGGCAT GAAGATTATA GTTTGTTCCG AAAAGTGTCT 
GTGTATCTCC TAACAGGCCT AGAACTCTAT CAAAAAGGAA AGTACCAAGA GGCACTTTCC 
TACCTGGTAT ATGCCTACCA GAGCAATGCT GCCCTGCTGA TGAAGGGGCC CCGCCGGGGG 
GTCAAAGAAT CCGTGATTGC TTTATACCGA AGAAAATGCC TTCTGGAGCT GAATGCCAAA 
GCAGCTTCTC TTTTTGAAAC AAATGATGAT CACTCCGTAA CTGAGGGCAT TAATGTGATG 
AATGAACTGA TCATCCCCTG CATTCACCTT ATCATTAATA ATGACATTTC CAAGGATGAT 
CTGGATGCCA TTGAGGTCAT GAGAAACCAT TGGTGCTCTT ACCTTGGGCA AGATATTGCA 
GAAAATCTGC AGCTGTGCCT AGGGGAGTTT CTACCCAGAC TTCTAGATCC TTCTGCAGAA 
ATCATCGTCT TGAAAGAGCC TCCAACTATT CGACCCAATT CTCCCTATGA CCTATGTAGC 
CGATTTGCAG CTGTCATGGA GTCAATTCAG GGAGTTTCAA CTGTGACAGT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005271636.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271693.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

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CloneID	OHu45848
Clone ID Related Accession (Same CDS sequence)	XM_005271636.2
Accession Version	XM_005271636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005271636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGCTT AAAGAAGCTG AACCCAAGAA GCCCATGCCC 
CAGGAAACAA ACCTTGCAGA GCAGTCAGAA CAGCCCCCAA AGGCTAATGA TGCAGAGTCT 
ACTGCCCAGC CTAATTCTGA GGTCTCTGAA GTCGAGATTC CCAGTGTGGG AAGGATTCTG 
GTTAGATCTG ATGCAGATGG ATATGATGAG GAGGTGATGC TGAGCCCTGC CATGCAAGGG 
GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG 
CTGATTAAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271693.1
CDS	66..3308
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_005271636.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271636.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGCTT AAAGAAGCTG AACCCAAGAA GCCCATGCCC 
CAGGAAACAA ACCTTGCAGA GCAGTCAGAA CAGCCCCCAA AGGCTAATGA TGCAGAGTCT 
ACTGCCCAGC CTAATTCTGA GGTCTCTGAA GTCGAGATTC CCAGTGTGGG AAGGATTCTG 
GTTAGATCTG ATGCAGATGG ATATGATGAG GAGGTGATGC TGAGCCCTGC CATGCAAGGG 
GTCATCCTGG CCATAGCTAA AGCCCGTCAG ACCTTTGACC GAGATGGGTC TGAAGCAGGG 
CTGATTAAGG CATTCCATGA AGAATACTCC AGGCTCTATC AGCTTGCCAA AGAGACCCCC 
ACCTCTCACA GTGATCCTCG ACTTCAGCAT GTCCTTGTCT ACTTTTTCCA AAATGAAGCA 
CCCAAAAGGG TAGTAGAACG AACCCTTCTG GAACAGTTTG CAGATAAAAA TCTTAGCTAT 
GATGAAAGAT CAATCAGCAT TATGAAGGTG GCTCAAGCGA AACTGAAGGA AATTGGTCCA 
GATGACATGA ATATGGAAGA GTACAAGAAG TGGCATGAAG ATTATAGTTT GTTCCGAAAA 
GTGTCTGTGT ATCTCCTAAC AGGCCTAGAA CTCTATCAAA AAGGAAAGTA CCAAGAGGCA 
CTTTCCTACC TGGTATATGC CTACCAGAGC AATGCTGCCC TGCTGATGAA GGGGCCCCGC 
CGGGGGGTCA AAGAATCCGT GATTGCTTTA TACCGAAGAA AATGCCTTCT GGAGCTGAAT 
GCCAAAGCAG CTTCTCTTTT TGAAACAAAT GATGATCACT CCGTAACTGA GGGCATTAAT 
GTGATGAATG AACTGATCAT CCCCTGCATT CACCTTATCA TTAATAATGA CATTTCCAAG 
GATGATCTGG ATGCCATTGA GGTCATGAGA AACCATTGGT GCTCTTACCT TGGGCAAGAT 
ATTGCAGAAA ATCTGCAGCT GTGCCTAGGG GAGTTTCTAC CCAGACTTCT AGATCCTTCT 
GCAGAAATCA TCGTCTTGAA AGAGCCTCCA ACTATTCGAC CCAATTCTCC CTATGACCTA 
TGTAGCCGAT TTGCAGCTGT CATGGAGTCA ATTCAGGGAG TTTCAACTGT GACAGTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_005271638.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271695.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu45850
Clone ID Related Accession (Same CDS sequence)	XM_005271638.2
Accession Version	XM_005271638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005271638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGGTAT GTGAAATATG GCTCAGGCCC AGCTCGGTTC 
CCGCTCCCGG ACATGCTGAA ATATGTTATT GAATTTGCTA GTACAAAACC TGCCTCAGAA 
AGCTGTCCAC CTGAAAGTGA CACACATATG ACATTACCAC TTTCTTCAGT GCACTGCTCG 
GTTTCTGACC AGACATCCAA GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGGCA TTCCATGAAG AATACTCCAG GCTCTATCAG 
CTTGCCAAAG AGACCCCCAC CTCTCACAGT GATCCTCGAC TTCAGCATGT CCTTGTCTAC 
TTTTTCCAAA ATGAAGCACC CAAAAGGGTA GTAGAACGAA CCCTTCTGGA ACAGTTTGCA 
GATAAAAATC TTAGCTATGA TGAAAGATCA ATCAGCATTA TGAAGGTGGC TCAAGCGAAA 
CTGAAGGAAA TTGGTCCAGA TGACATGAAT ATGGAAGAGT ACAAGAAGTG GCATGAAGAT 
TATAGTTTGT TCCGAAAAGT GTCTGTGTAT CTCCTAACAG GCCTAGAACT CTATCAAAAA 
GGAAAGTACC AAGAGGCACT TTCCTACCTG GTATATGCCT ACCAGAGCAA TGCTGCCCTG 
CTGATGAAGG GGCCCCGCCG GGGGGTCAAA GAATCCGTGA TTGCTTTATA CCGAAGAAAA 
TGCCTTCTGG AGCTGAATGC CAAAGCAGCT TCTCTTTTTG AAACAAATGA TGATCACTCC 
GTAACTGAGG GCATTAATGT GATGAATGAA CTGATCATCC CCTGCATTCA CCTTATCATT 
AATAATGACA TTTCCAAGGA TGATCTGGAT GCCATTGAGG TCATGAGAAA CCATTGGTGC 
TCTTACCTTG GGCAAGATAT TGCAGAAAAT CTGCAGCTGT GCCTAGGGGA GTTTCTACCC 
AGACTTCTAG ATCCTTCTGC AGAAATCATC GTCTTGAAAG AGCCTCCAAC TATTCGACCC 
AATTCTCCCT ATGACCTATG TAGCCGATTT GCAGCTGTCA TGGAGTCAAT TCAGGGAGTT 
TCAACTGTGA CAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271695.1
CDS	66..3206
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_005271638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271638.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGAGC 
TGCCAAATGC TGTTAAATCA ACTGAGAGAA ATCACAGGCA TTCAGGACCC TTCCTTTCTC 
CATGAAGCTC TGAAGGCCAG TAATGGTGAC ATTACTCAGG CAGTCAGCCT TCTCACTGAT 
GAGAGAGTTA AGGAGCCCAG TCAAGACACT GTTGCTACAG AACCATCTGA AGTAGAGGGG 
AGTGCTGCCA ACAAGGAAGT ATTAGCAAAA GTTATAGACC TTACTCATGA TAACAAAGAT 
GATCTTCAGG CTGCCATTGC TTTGAGTCTA CTGGAGTCTC CCAAAATTCA AGCTGATGGA 
AGAGATCTTA ACAGGATGCA TGAAGCAACC TCTGCAGAAA CTAAACGCTC AAAGAGAAAA 
CGCTGTGAAG TCTGGGGAGA AAACCCCAAT CCCAATGACT GGAGGAGAGT TGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAATACA TGTTGGTTTA GTGCTGTTAT TCAGTCTCTC 
TTTCAATTGC CTGAATTTCG AAGACTTGTT CTCAGTTATA GTCTGCCACA AAATGTACTT 
GAAAATTGTC GAAGTCATAC AGAAAAGAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTGTTTGCTC TAATGATGGG ATCAAATAGA AAATTTGTAG ACCCGTCTGC AGCCCTGGAT 
CTATTAAAGG GAGCATTCCG ATCATCTGAG GAACAGCAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT AGAGGACGCA TTCCAGCTAG CTGTTAATGT TAACAGCAGT 
CCCAGGAACA AATCTGAAAA TCCAATGGTG CAGCTGTTCT ATGGTACTTT CCTGACTGAA 
GGGGTTCGTG AAGGAAAACC CTTTTGTAAC AATGAGACCT TCGGCCAGTA TCCTCTTCAG 
GTAAACGGTT ATCGCAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA GGGTGATGTT 
GAGCTTCTTC CCTCCGATCA CTCGGTGAAG TATGGACAAG AGCGTTGGTT TACAAAGCTA 
CCTCCAGTGT TGACCTTTGA ACTCTCAAGA TTTGAGTTTA ATCAGTCCCT TGGGCAGCCA 
GAGAAAATTC ACAATAAGCT GGAATTTCCT CAGATTATTT ATATGGACAG GTACATGTAC 
AGGAGCAAGG AGCTTATTCG AAATAAGAGA GAGTGTATTC GAAAGTTGAA GGAGGAAATA 
AAAATTCTGC AGCAAAAATT GGAAAGGTAT GTGAAATATG GCTCAGGCCC AGCTCGGTTC 
CCGCTCCCGG ACATGCTGAA ATATGTTATT GAATTTGCTA GTACAAAACC TGCCTCAGAA 
AGCTGTCCAC CTGAAAGTGA CACACATATG ACATTACCAC TTTCTTCAGT GCACTGCTCG 
GTTTCTGACC AGACATCCAA GGAAAGTACA AGTACAGAAA GCTCTTCTCA GGATGTTGAA 
AGTACCTTTT CTTCTCCTGA AGATTCTTTA CCCAAGTCTA AACCACTGAC ATCTTCTCGG 
TCTTCCATGG AAATGCCTTC ACAGCCAGCT CCACGAACAG TCACAGATGA GGAGATAAAT 
TTTGTTAAGA CCTGTCTTCA GAGATGGAGG AGTGAGATTG AACAAGATAT ACAAGATTTA 
AAGACTTGTA TTGCAAGTAC TACTCAGACT ATTGAACAGA TGTACTGCGA TCCTCTCCTT 
CGTCAGGTGC CTTATCGCTT GCATGCAGTT CTTGTTCATG AAGGACAAGC AAATGCTGGA 
CACTATTGGG CCTATATCTA TAATCAACCC CGACAGAGCT GGCTCAAGTA CAATGACATC 
TCTGTTACTG AATCTTCCTG GGAAGAAGTT GAAAGAGATT CCTATGGAGG CCTGAGAAAT 
GTTAGTGCTT ACTGTCTGAT GTACATTAAT GACAAACTAC CCTACTTCAA TGCAGAGGCA 
GCCCCAACTG AATCAGATCA AATGTCAGAA GTGGAAGCCC TATCTGTGGA ACTCAAGCAT 
TACATTCAGG AGGATAACTG GCGGTTTGAG CAGGAAGTAG AGGAGTGGGA AGAAGAGCAG 
TCTTGCAAAA TCCCTCAAAT GGAGTCCTCC ACCAACTCCT CATCACAGGA CTACTCTACA 
TCACAAGAGC CTTCAGTAGC CTCTTCTCAT GGGGTTCGCT GCTTGTCGTC TGAGCATGCT 
GTGATTGTAA AGGAGCAAAC TGCCCAGGCT ATTGCAAACA CAGCCCGTGC CTATGAGAAG 
AGCGGTGTAG AAGCGGCACT GAGTGAGGCA TTCCATGAAG AATACTCCAG GCTCTATCAG 
CTTGCCAAAG AGACCCCCAC CTCTCACAGT GATCCTCGAC TTCAGCATGT CCTTGTCTAC 
TTTTTCCAAA ATGAAGCACC CAAAAGGGTA GTAGAACGAA CCCTTCTGGA ACAGTTTGCA 
GATAAAAATC TTAGCTATGA TGAAAGATCA ATCAGCATTA TGAAGGTGGC TCAAGCGAAA 
CTGAAGGAAA TTGGTCCAGA TGACATGAAT ATGGAAGAGT ACAAGAAGTG GCATGAAGAT 
TATAGTTTGT TCCGAAAAGT GTCTGTGTAT CTCCTAACAG GCCTAGAACT CTATCAAAAA 
GGAAAGTACC AAGAGGCACT TTCCTACCTG GTATATGCCT ACCAGAGCAA TGCTGCCCTG 
CTGATGAAGG GGCCCCGCCG GGGGGTCAAA GAATCCGTGA TTGCTTTATA CCGAAGAAAA 
TGCCTTCTGG AGCTGAATGC CAAAGCAGCT TCTCTTTTTG AAACAAATGA TGATCACTCC 
GTAACTGAGG GCATTAATGT GATGAATGAA CTGATCATCC CCTGCATTCA CCTTATCATT 
AATAATGACA TTTCCAAGGA TGATCTGGAT GCCATTGAGG TCATGAGAAA CCATTGGTGC 
TCTTACCTTG GGCAAGATAT TGCAGAAAAT CTGCAGCTGT GCCTAGGGGA GTTTCTACCC 
AGACTTCTAG ATCCTTCTGC AGAAATCATC GTCTTGAAAG AGCCTCCAAC TATTCGACCC 
AATTCTCCCT ATGACCTATG TAGCCGATTT GCAGCTGTCA TGGAGTCAAT TCAGGGAGTT 
TCAACTGTGA CAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_011542936.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541238.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu70766
Clone ID Related Accession (Same CDS sequence)	XM_011542936.2
Accession Version	XM_011542936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGCA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGC TTAAAGAAGC TGAACCCAAG AAGCCCATGC CCCAGGAAAC AAACCTTGCA 
GAGCAGTCAG AACAGCCCCC AAAGGCTAAT GATGCAGAGT CTACTGCCCA GCCTAATTCT 
GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG GGAAGGATTC TGGTTAGATC TGATGCAGAT 
GGATATGATG AGGAGGTGAT GCTGAGCCCT GCCATGCAAG GGGTCATCCT GGCCATAGCT 
AAAGCCCGTC AGACCTTTGA CCGAGATGGG TCTGAAGCAG GGCTGATTAA GGCATTCCAT 
GAAGAATACT CCAGGCTCTA TCAGCTTGCC AAAGAGACCC CCACCTCTCA CAGTGATCCT 
CGACTTCAGC ATGTCCTTGT CTACTTTTTC CAAAATGAAG CACCCAAAAG GGTAGTAGAA 
CGAACCCTTC TGGAACAGTT TGCAGATAAA AATCTTAGCT ATGATGAAAG ATCAATCAGC 
ATTATGAAGG TGGCTCAAGC GAAACTGAAG GAAATTGGTC CAGATGACAT GAATATGGAA 
GAGTACAAGA AGTGGCATGA AGATTATAGT TTGTTCCGAA AAGTGTCTGT GTATCTCCTA 
ACAGGCCTAG AACTCTATCA AAAAGGAAAG TACCAAGAGG CACTTTCCTA CCTGGTATAT 
GCCTACCAGA GCAATGCTGC CCTGCTGATG AAGGGGCCCC GCCGGGGGGT CAAAGAATCC 
GTGATTGCTT TATACCGAAG AAAATGCCTT CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT 
TTTGAAACAA ATGATGATCA CTCCGTAACT GAGGGCATTA ATGTGATGAA TGAACTGATC 
ATCCCCTGCA TTCACCTTAT CATTAATAAT GACATTTCCA AGGATGATCT GGATGCCATT 
GAGGTCATGA GAAACCATTG GTGCTCTTAC CTTGGGCAAG ATATTGCAGA AAATCTGCAG 
CTGTGCCTAG GGGAGTTTCT ACCCAGACTT CTAGATCCTT CTGCAGAAAT CATCGTCTTG 
AAAGAGCCTC CAACTATTCG ACCCAATTCT CCCTATGACC TATGTAGCCG ATTTGCAGCT 
GTCATGGAGT CAATTCAGGG AGTTTCAACT GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541238.1
CDS	63..3407
Translation	MTAELQQDDAAGAADGHGSASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_011542936.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542936.2

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCCGGCGCGG CAGACGGCCA CGGCTCGGCC 
AGTAATGGTG ACATTACTCA GGCAGTCAGC CTTCTCACTG ATGAGAGAGT TAAGGAGCCC 
AGTCAAGACA CTGTTGCTAC AGAACCATCT GAAGTAGAGG GGAGTGCTGC CAACAAGGAA 
GTATTAGCAA AAGTTATAGA CCTTACTCAT GATAACAAAG ATGATCTTCA GGCTGCCATT 
GCTTTGAGTC TACTGGAGTC TCCCAAAATT CAAGCTGATG GAAGAGATCT TAACAGGATG 
CATGAAGCAA CCTCTGCAGA AACTAAACGC TCAAAGAGAA AACGCTGTGA AGTCTGGGGA 
GAAAACCCCA ATCCCAATGA CTGGAGGAGA GTTGATGGTT GGCCAGTTGG GCTGAAAAAT 
GTTGGCAATA CATGTTGGTT TAGTGCTGTT ATTCAGTCTC TCTTTCAATT GCCTGAATTT 
CGAAGACTTG TTCTCAGTTA TAGTCTGCCA CAAAATGTAC TTGAAAATTG TCGAAGTCAT 
ACAGAAAAGA GAAATATCAT GTTTATGCAA GAGCTTCAGT ATTTGTTTGC TCTAATGATG 
GGATCAAATA GAAAATTTGT AGACCCGTCT GCAGCCCTGG ATCTATTAAA GGGAGCATTC 
CGATCATCTG AGGAACAGCA GCAAGATGTG AGTGAATTCA CACACAAGCT CCTGGATTGG 
CTAGAGGACG CATTCCAGCT AGCTGTTAAT GTTAACAGCA GTCCCAGGAA CAAATCTGAA 
AATCCAATGG TGCAGCTGTT CTATGGTACT TTCCTGACTG AAGGGGTTCG TGAAGGAAAA 
CCCTTTTGTA ACAATGAGAC CTTCGGCCAG TATCCTCTTC AGGTAAACGG TTATCGCAAC 
TTAGACGAGT GTTTGGAAGG GGCCATGGTG GAGGGTGATG TTGAGCTTCT TCCCTCCGAT 
CACTCGGTGA AGTATGGACA AGAGCGTTGG TTTACAAAGC TACCTCCAGT GTTGACCTTT 
GAACTCTCAA GATTTGAGTT TAATCAGTCC CTTGGGCAGC CAGAGAAAAT TCACAATAAG 
CTGGAATTTC CTCAGATTAT TTATATGGAC AGGTACATGT ACAGGAGCAA GGAGCTTATT 
CGAAATAAGA GAGAGTGTAT TCGAAAGTTG AAGGAGGAAA TAAAAATTCT GCAGCAAAAA 
TTGGAAAGGT ATGTGAAATA TGGCTCAGGC CCAGCTCGGT TCCCGCTCCC GGACATGCTG 
AAATATGTTA TTGAATTTGC TAGTACAAAA CCTGCCTCAG AAAGCTGTCC ACCTGAAAGT 
GACACACATA TGACATTACC ACTTTCTTCA GTGCACTGCT CGGTTTCTGA CCAGACATCC 
AAGGAAAGTA CAAGTACAGA AAGCTCTTCT CAGGATGTTG AAAGTACCTT TTCTTCTCCT 
GAAGATTCTT TACCCAAGTC TAAACCACTG ACATCTTCTC GGTCTTCCAT GGAAATGCCT 
TCACAGCCAG CTCCACGAAC AGTCACAGAT GAGGAGATAA ATTTTGTTAA GACCTGTCTT 
CAGAGATGGA GGAGTGAGAT TGAACAAGAT ATACAAGATT TAAAGACTTG TATTGCAAGT 
ACTACTCAGA CTATTGAACA GATGTACTGC GATCCTCTCC TTCGTCAGGT GCCTTATCGC 
TTGCATGCAG TTCTTGTTCA TGAAGGACAA GCAAATGCTG GACACTATTG GGCCTATATC 
TATAATCAAC CCCGACAGAG CTGGCTCAAG TACAATGACA TCTCTGTTAC TGAATCTTCC 
TGGGAAGAAG TTGAAAGAGA TTCCTATGGA GGCCTGAGAA ATGTTAGTGC TTACTGTCTG 
ATGTACATTA ATGACAAACT ACCCTACTTC AATGCAGAGG CAGCCCCAAC TGAATCAGAT 
CAAATGTCAG AAGTGGAAGC CCTATCTGTG GAACTCAAGC ATTACATTCA GGAGGATAAC 
TGGCGGTTTG AGCAGGAAGT AGAGGAGTGG GAAGAAGAGC AGTCTTGCAA AATCCCTCAA 
ATGGAGTCCT CCACCAACTC CTCATCACAG GACTACTCTA CATCACAAGA GCCTTCAGTA 
GCCTCTTCTC ATGGGGTTCG CTGCTTGTCG TCTGAGCATG CTGTGATTGT AAAGGAGCAA 
ACTGCCCAGG CTATTGCAAA CACAGCCCGT GCCTATGAGA AGAGCGGTGT AGAAGCGGCA 
CTGAGTGAGC TTAAAGAAGC TGAACCCAAG AAGCCCATGC CCCAGGAAAC AAACCTTGCA 
GAGCAGTCAG AACAGCCCCC AAAGGCTAAT GATGCAGAGT CTACTGCCCA GCCTAATTCT 
GAGGTCTCTG AAGTCGAGAT TCCCAGTGTG GGAAGGATTC TGGTTAGATC TGATGCAGAT 
GGATATGATG AGGAGGTGAT GCTGAGCCCT GCCATGCAAG GGGTCATCCT GGCCATAGCT 
AAAGCCCGTC AGACCTTTGA CCGAGATGGG TCTGAAGCAG GGCTGATTAA GGCATTCCAT 
GAAGAATACT CCAGGCTCTA TCAGCTTGCC AAAGAGACCC CCACCTCTCA CAGTGATCCT 
CGACTTCAGC ATGTCCTTGT CTACTTTTTC CAAAATGAAG CACCCAAAAG GGTAGTAGAA 
CGAACCCTTC TGGAACAGTT TGCAGATAAA AATCTTAGCT ATGATGAAAG ATCAATCAGC 
ATTATGAAGG TGGCTCAAGC GAAACTGAAG GAAATTGGTC CAGATGACAT GAATATGGAA 
GAGTACAAGA AGTGGCATGA AGATTATAGT TTGTTCCGAA AAGTGTCTGT GTATCTCCTA 
ACAGGCCTAG AACTCTATCA AAAAGGAAAG TACCAAGAGG CACTTTCCTA CCTGGTATAT 
GCCTACCAGA GCAATGCTGC CCTGCTGATG AAGGGGCCCC GCCGGGGGGT CAAAGAATCC 
GTGATTGCTT TATACCGAAG AAAATGCCTT CTGGAGCTGA ATGCCAAAGC AGCTTCTCTT 
TTTGAAACAA ATGATGATCA CTCCGTAACT GAGGGCATTA ATGTGATGAA TGAACTGATC 
ATCCCCTGCA TTCACCTTAT CATTAATAAT GACATTTCCA AGGATGATCT GGATGCCATT 
GAGGTCATGA GAAACCATTG GTGCTCTTAC CTTGGGCAAG ATATTGCAGA AAATCTGCAG 
CTGTGCCTAG GGGAGTTTCT ACCCAGACTT CTAGATCCTT CTGCAGAAAT CATCGTCTTG 
AAAGAGCCTC CAACTATTCG ACCCAATTCT CCCTATGACC TATGTAGCCG ATTTGCAGCT 
GTCATGGAGT CAATTCAGGG AGTTTCAACT GTGACAGTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_011542938.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541240.1 Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X14, mRNA.

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CloneID	OHu70768
Clone ID Related Accession (Same CDS sequence)	XM_011542938.1
Accession Version	XM_011542938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GCAGTCCCAG GAACAAATCT 
GAAAATCCAA TGGTGCAGCT GTTCTATGGT ACTTTCCTGA CTGAAGGGGT TCGTGAAGGA 
AAACCCTTTT GTAACAATGA GACCTTCGGC CAGTATCCTC TTCAGGTAAA CGGTTATCGC 
AACTTAGACG AGTGTTTGGA AGGGGCCATG GTGGAGGGTG ATGTTGAGCT TCTTCCCTCC 
GATCACTCGG TGAAGTATGG ACAAGAGCGT TGGTTTACAA AGCTACCTCC AGTGTTGACC 
TTTGAACTCT CAAGATTTGA GTTTAATCAG TCCCTTGGGC AGCCAGAGAA AATTCACAAT 
AAGCTGGAAT TTCCTCAGAT TATTTATATG GACAGGTACA TGTACAGGAG CAAGGAGCTT 
ATTCGAAATA AGAGAGAGTG TATTCGAAAG TTGAAGGAGG AAATAAAAAT TCTGCAGCAA 
AAATTGGAAA GGTATGTGAA ATATGGCTCA GGCCCAGCTC GGTTCCCGCT CCCGGACATG 
CTGAAATATG TTATTGAATT TGCTAGTACA AAACCTGCCT CAGAAAGCTG TCCACCTGAA 
AGTGACACAC ATATGACATT ACCACTTTCT TCAGTGCACT GCTCGGTTTC TGACCAGACA 
TCCAAGGAAA GTACAAGTAC AGAAAGCTCT TCTCAGGATG TTGAAAGTAC CTTTTCTTCT 
CCTGAAGATT CTTTACCCAA GTCTAAACCA CTGACATCTT CTCGGTCTTC CATGGAAATG 
CCTTCACAGC CAGCTCCACG AACAGTCACA GATGAGGAGA TAAATTTTGT TAAGACCTGT 
CTTCAGAGAT GGAGGAGTGA GATTGAACAA GATATACAAG ATTTAAAGAC TTGTATTGCA 
AGTACTACTC AGACTATTGA ACAGATGTAC TGCGATCCTC TCCTTCGTCA GGTGCCTTAT 
CGCTTGCATG CAGTTCTTGT TCATGAAGGA CAAGCAAATG CTGGACACTA TTGGGCCTAT 
ATCTATAATC AACCCCGACA GAGCTGGCTC AAGTACAATG ACATCTCTGT TACTGAATCT 
TCCTGGGAAG AAGTTGAAAG AGATTCCTAT GGAGGCCTGA GAAATGTTAG TGCTTACTGT 
CTGATGTACA TTAATGACAA ACTACCCTAC TTCAATGCAG AGGCAGCCCC AACTGAATCA 
GATCAAATGT CAGAAGTGGA AGCCCTATCT GTGGAACTCA AGCATTACAT TCAGGAGGAT 
AACTGGCGGT TTGAGCAGGA AGTAGAGGAG TGGGAAGAAG AGCAGTCTTG CAAAATCCCT 
CAAATGGAGT CCTCCACCAA CTCCTCATCA CAGGACTACT CTACATCACA AGAGCCTTCA 
GTAGCCTCTT CTCATGGGGT TCGCTGCTTG TCGTCTGAGC ATGCTGTGAT TGTAAAGGAG 
CAAACTGCCC AGGCTATTGC AAACACAGCC CGTGCCTATG AGAAGAGCGG TGTAGAAGCG 
GCACTGAGTG AGCTTAAAGA AGCTGAACCC AAGAAGCCCA TGCCCCAGGA AACAAACCTT 
GCAGAGCAGT CAGAACAGCC CCCAAAGGCT AATGATGCAG AGTCTACTGC CCAGCCTAAT 
TCTGAGGTCT CTGAAGTCGA GATTCCCAGT GTGGGAAGGA TTCTGGTTAG ATCTGATGCA 
GATGGATATG ATGAGGAGGT GATGCTGAGC CCTGCCATGC AAGGGGTCAT CCTGGCCATA 
GCTAAAGCCC GTCAGACCTT TGACCGAGAT GGGTCTGAAG CAGGGCTGAT TAAGGCATTC 
CATGAAGAAT ACTCCAGGCT CTATCAGCTT GCCAAAGAGA CCCCCACCTC TCACAGTGAT 
CCTCGACTTC AGCATGTCCT TGTCTACTTT TTCCAAAATG AAGCACCCAA AAGGGTAGTA 
GAACGAACCC TTCTGGAACA GTTTGCAGAT AAAAATCTTA GCTATGATGA AAGATCAATC 
AGCATTATGA AGGTGGCTCA AGCGAAACTG AAGGAAATTG GTCCAGATGA CATGAATATG 
GAAGAGTACA AGAAGTGGCA TGAAGATTAT AGTTTGTTCC GAAAAGTGTC TGTGTATCTC 
CTAACAGGCC TAGAACTCTA TCAAAAAGGA AAGTACCAAG AGGCACTTTC CTACCTGGTA 
TATGCCTACC AGAGCAATGC TGCCCTGCTG ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA 
TCCGTGATTG CTTTATACCG AAGAAAATGC CTTCTGGAGC TGAATGCCAA AGCAGCTTCT 
CTTTTTGAAA CAAATGATGA TCACTCCGTA ACTGAGGGCA TTAATGTGAT GAATGAACTG 
ATCATCCCCT GCATTCACCT TATCATTAAT AATGACATTT CCAAGGATGA TCTGGATGCC 
ATTGAGGTCA TGAGAAACCA TTGGTGCTCT TACCTTGGGC AAGATATTGC AGAAAATCTG 
CAGCTGTGCC TAGGGGAGTT TCTACCCAGA CTTCTAGATC CTTCTGCAGA AATCATCGTC 
TTGAAAGAGC CTCCAACTAT TCGACCCAAT TCTCCCTATG ACCTATGTAG CCGATTTGCA 
GCTGTCATGG AGTCAATTCA GGGAGTTTCA ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541240.1
CDS	234..3281
Translation	MHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_011542938.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542938.1

ORF Insert Sequence:

ATGCATGAAG CAACCTCTGC AGAAACTAAA CGCTCAAAGA GAAAACGCTG TGAAGTCTGG 
GGAGAAAACC CCAATCCCAA TGACTGGAGG AGAGTTGATG GTTGGCCAGT TGGGCTGAAA 
AATGTTGGCA ATACATGTTG GTTTAGTGCT GTTATTCAGT CTCTCTTTCA ATTGCCTGAA 
TTTCGAAGAC TTGTTCTCAG TTATAGTCTG CCACAAAATG TACTTGAAAA TTGTCGAAGT 
CATACAGAAA AGAGAAATAT CATGTTTATG CAAGAGCTTC AGTATTTGTT TGCTCTAATG 
ATGGGATCAA ATAGAAAATT TGTAGACCCG TCTGCAGCCC TGGATCTATT AAAGGGAGCA 
TTCCGATCAT CTGAGGAACA GCAGCAAGAT GTGAGTGAAT TCACACACAA GCTCCTGGAT 
TGGCTAGAGG ACGCATTCCA GCTAGCTGTT AATGTTAACA GCAGTCCCAG GAACAAATCT 
GAAAATCCAA TGGTGCAGCT GTTCTATGGT ACTTTCCTGA CTGAAGGGGT TCGTGAAGGA 
AAACCCTTTT GTAACAATGA GACCTTCGGC CAGTATCCTC TTCAGGTAAA CGGTTATCGC 
AACTTAGACG AGTGTTTGGA AGGGGCCATG GTGGAGGGTG ATGTTGAGCT TCTTCCCTCC 
GATCACTCGG TGAAGTATGG ACAAGAGCGT TGGTTTACAA AGCTACCTCC AGTGTTGACC 
TTTGAACTCT CAAGATTTGA GTTTAATCAG TCCCTTGGGC AGCCAGAGAA AATTCACAAT 
AAGCTGGAAT TTCCTCAGAT TATTTATATG GACAGGTACA TGTACAGGAG CAAGGAGCTT 
ATTCGAAATA AGAGAGAGTG TATTCGAAAG TTGAAGGAGG AAATAAAAAT TCTGCAGCAA 
AAATTGGAAA GGTATGTGAA ATATGGCTCA GGCCCAGCTC GGTTCCCGCT CCCGGACATG 
CTGAAATATG TTATTGAATT TGCTAGTACA AAACCTGCCT CAGAAAGCTG TCCACCTGAA 
AGTGACACAC ATATGACATT ACCACTTTCT TCAGTGCACT GCTCGGTTTC TGACCAGACA 
TCCAAGGAAA GTACAAGTAC AGAAAGCTCT TCTCAGGATG TTGAAAGTAC CTTTTCTTCT 
CCTGAAGATT CTTTACCCAA GTCTAAACCA CTGACATCTT CTCGGTCTTC CATGGAAATG 
CCTTCACAGC CAGCTCCACG AACAGTCACA GATGAGGAGA TAAATTTTGT TAAGACCTGT 
CTTCAGAGAT GGAGGAGTGA GATTGAACAA GATATACAAG ATTTAAAGAC TTGTATTGCA 
AGTACTACTC AGACTATTGA ACAGATGTAC TGCGATCCTC TCCTTCGTCA GGTGCCTTAT 
CGCTTGCATG CAGTTCTTGT TCATGAAGGA CAAGCAAATG CTGGACACTA TTGGGCCTAT 
ATCTATAATC AACCCCGACA GAGCTGGCTC AAGTACAATG ACATCTCTGT TACTGAATCT 
TCCTGGGAAG AAGTTGAAAG AGATTCCTAT GGAGGCCTGA GAAATGTTAG TGCTTACTGT 
CTGATGTACA TTAATGACAA ACTACCCTAC TTCAATGCAG AGGCAGCCCC AACTGAATCA 
GATCAAATGT CAGAAGTGGA AGCCCTATCT GTGGAACTCA AGCATTACAT TCAGGAGGAT 
AACTGGCGGT TTGAGCAGGA AGTAGAGGAG TGGGAAGAAG AGCAGTCTTG CAAAATCCCT 
CAAATGGAGT CCTCCACCAA CTCCTCATCA CAGGACTACT CTACATCACA AGAGCCTTCA 
GTAGCCTCTT CTCATGGGGT TCGCTGCTTG TCGTCTGAGC ATGCTGTGAT TGTAAAGGAG 
CAAACTGCCC AGGCTATTGC AAACACAGCC CGTGCCTATG AGAAGAGCGG TGTAGAAGCG 
GCACTGAGTG AGCTTAAAGA AGCTGAACCC AAGAAGCCCA TGCCCCAGGA AACAAACCTT 
GCAGAGCAGT CAGAACAGCC CCCAAAGGCT AATGATGCAG AGTCTACTGC CCAGCCTAAT 
TCTGAGGTCT CTGAAGTCGA GATTCCCAGT GTGGGAAGGA TTCTGGTTAG ATCTGATGCA 
GATGGATATG ATGAGGAGGT GATGCTGAGC CCTGCCATGC AAGGGGTCAT CCTGGCCATA 
GCTAAAGCCC GTCAGACCTT TGACCGAGAT GGGTCTGAAG CAGGGCTGAT TAAGGCATTC 
CATGAAGAAT ACTCCAGGCT CTATCAGCTT GCCAAAGAGA CCCCCACCTC TCACAGTGAT 
CCTCGACTTC AGCATGTCCT TGTCTACTTT TTCCAAAATG AAGCACCCAA AAGGGTAGTA 
GAACGAACCC TTCTGGAACA GTTTGCAGAT AAAAATCTTA GCTATGATGA AAGATCAATC 
AGCATTATGA AGGTGGCTCA AGCGAAACTG AAGGAAATTG GTCCAGATGA CATGAATATG 
GAAGAGTACA AGAAGTGGCA TGAAGATTAT AGTTTGTTCC GAAAAGTGTC TGTGTATCTC 
CTAACAGGCC TAGAACTCTA TCAAAAAGGA AAGTACCAAG AGGCACTTTC CTACCTGGTA 
TATGCCTACC AGAGCAATGC TGCCCTGCTG ATGAAGGGGC CCCGCCGGGG GGTCAAAGAA 
TCCGTGATTG CTTTATACCG AAGAAAATGC CTTCTGGAGC TGAATGCCAA AGCAGCTTCT 
CTTTTTGAAA CAAATGATGA TCACTCCGTA ACTGAGGGCA TTAATGTGAT GAATGAACTG 
ATCATCCCCT GCATTCACCT TATCATTAAT AATGACATTT CCAAGGATGA TCTGGATGCC 
ATTGAGGTCA TGAGAAACCA TTGGTGCTCT TACCTTGGGC AAGATATTGC AGAAAATCTG 
CAGCTGTGCC TAGGGGAGTT TCTACCCAGA CTTCTAGATC CTTCTGCAGA AATCATCGTC 
TTGAAAGAGC CTCCAACTAT TCGACCCAAT TCTCCCTATG ACCTATGTAG CCGATTTGCA 
GCTGTCATGG AGTCAATTCA GGGAGTTTCA ACTGTGACAG TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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USP28 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: