USP28 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015298017.1	XP_015153503.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
XM_015298019.2	XP_015153505.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X10
XM_015298014.1	XP_015153500.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
XM_015298015.1	XP_015153501.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
XM_025143198.1	XP_024998966.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
XM_025143201.1	XP_024998969.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
XM_015298012.2	XP_015153498.2	ubiquitin carboxyl-terminal hydrolase 28 isoform X9
XM_015298016.1	XP_015153502.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
XM_015298019.1	XP_015153505.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
XM_025143197.1	XP_024998965.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
XM_025143199.1	XP_024998967.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
XM_025143200.1	XP_024998968.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
XM_025143203.1	XP_024998971.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X8
XM_015298012.1	XP_015153498.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
XM_025143196.1	XP_024998964.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
XM_025143202.1	XP_024998970.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X7
XM_015298013.1	XP_015153499.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
NM_001031496.1	NP_001026667.1	ubiquitin carboxyl-terminal hydrolase 28

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa147539	XM_025143196.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa147540	XM_025143197.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa147541	XM_025143198.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa147542	XM_025143199.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa147543	XM_025143200.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa147544	XM_025143201.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa147545	XM_025143202.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa147546	XM_025143203.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa147547	XM_015298012.2 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa45821	XM_015298012.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa45821	XM_015298019.2 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa45822	XM_015298013.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa45823	XM_015298014.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa45824	XM_015298015.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa45825	XM_015298016.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa45826	XM_015298017.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa27290	NM_001031496.1 Latest version!	Gallus gallus ubiquitin specific peptidase 28 (USP28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OGa45821	XM_015298019.1	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

USP28 ( XM_025143196.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998964.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa147539
Clone ID Related Accession (Same CDS sequence)	XM_025143196.1
Accession Version	XM_025143196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGCCAA AGGAGGTAGA ACCAATGAAG GCCCAACTGG GAGAAACCAC CTTTACAGTT 
CAGGCAGAGC AACCTCAGGA TGCTAAAGAA GCAGAGCCCA CTGCCCAGAG TAGCTCACAG 
ATCTCGGAAG TGGAAATCCC CAGTGTGGGG AAGGTTCCTG TTAGATCTGA TGCAGATGGA 
TATAATGAGG AGGTGATGCT GAGTCCAGCC ATGCAAGGTG TCATCCTGGC TATTGCAAAA 
GCCCGCCAGA CCTTTGATCG TGATGGGTCT GAAGCGGGGC TCGTTAAGGC ATTCCACGAG 
GAGTACTCCA GACTCTATCT CCTTTCCAAA GAGACCCCGA CCCCTCAGAA CGATGCCCGC 
TTGCAACACG TGCTCGTTTA CTTCCTTCAG AACGATGCTC CCCAGCAGAT TGTGGAACGG 
ACCCTCCTCG AGCAGTTTGC AGACAAAAAC CTCAGCTACG ATGAAAGGTC CATTAGCATC 
ATGAAGGTAG CACGAGATAA ACTGAAAGAG ATCGGTCCTG ATGAGGTCAA TATGGAGGAG 
TATAAGAAGT GGCATGAGGA CTACAGTCTT TTTCGGAAGG TGTCTGTTTA CCTGCTTACA 
GGGTTGGAGC TGTACCAGAA TAGAAAATAC AAGGAGTCCC TGACCTACCT GATCTATGCC 
TACCAAAGCA ACACCACTCT GCTGAAGAAG GGAGCCAACA GAGGAGTGAA TGAATCGCTG 
ATCACCTTGT ATAGAAGAAA GTGTCTCTTG AAGCTGAATG AGGTGGCATC ATCTCTGTTT 
GTAAGCTGTG AGGAAGCCCA CGTGGCAGAG GGCATTAGCA TCCTGAATGA GCTGATCATC 
CCCTGTATGC ACCTCATTAA CAACTTTGAC ATCTCCAGAG AAGACATGGA TGCCATTGAG 
GTCATGAGAA ACCGCTGGTG CTCCTATCTG GGACGAGAAG ACATGGACGC AAGCCTGCAG 
ATCAGGCTGG GCGAGCTGCT CCCCCGCCTG CTGGACGGCT CCACGGAGGT GGTGGTGCTG 
AAGGAGCCCC CAAAGATCCG TCCCAACTCC CCATACGACC TCTGCAGCCG CTTTGCAGCG 
GTGATGGAGT CCATCCATGA CGCCTCGACC GTGACTGTGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998964.1
CDS	98..4222
Translation	METQRTRSVHGGTARVVCTGTQHARCAQRYSPYTACTGTQHTCSVHRGRECTGTQHTHGMHGDTGCPEAQHALGFSTHVVCIGTQRSQCAQRHSTHTACTGVQRRQGYSTQRMCRDAVCMGHSTHSMHRGTACMGTACTEMQCTQEHSTHRDTARTQCMLCAQGCHTRRTTACTGLQSSVVSTGMWHTRTELEHTPSLHTDTACTRMWAHSVCTFEPFRSFPQVQQEAWIIVHFAQDCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_025143196.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143196.1

ORF Insert Sequence:

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGCCAA AGGAGGTAGA ACCAATGAAG GCCCAACTGG GAGAAACCAC CTTTACAGTT 
CAGGCAGAGC AACCTCAGGA TGCTAAAGAA GCAGAGCCCA CTGCCCAGAG TAGCTCACAG 
ATCTCGGAAG TGGAAATCCC CAGTGTGGGG AAGGTTCCTG TTAGATCTGA TGCAGATGGA 
TATAATGAGG AGGTGATGCT GAGTCCAGCC ATGCAAGGTG TCATCCTGGC TATTGCAAAA 
GCCCGCCAGA CCTTTGATCG TGATGGGTCT GAAGCGGGGC TCGTTAAGGC ATTCCACGAG 
GAGTACTCCA GACTCTATCT CCTTTCCAAA GAGACCCCGA CCCCTCAGAA CGATGCCCGC 
TTGCAACACG TGCTCGTTTA CTTCCTTCAG AACGATGCTC CCCAGCAGAT TGTGGAACGG 
ACCCTCCTCG AGCAGTTTGC AGACAAAAAC CTCAGCTACG ATGAAAGGTC CATTAGCATC 
ATGAAGGTAG CACGAGATAA ACTGAAAGAG ATCGGTCCTG ATGAGGTCAA TATGGAGGAG 
TATAAGAAGT GGCATGAGGA CTACAGTCTT TTTCGGAAGG TGTCTGTTTA CCTGCTTACA 
GGGTTGGAGC TGTACCAGAA TAGAAAATAC AAGGAGTCCC TGACCTACCT GATCTATGCC 
TACCAAAGCA ACACCACTCT GCTGAAGAAG GGAGCCAACA GAGGAGTGAA TGAATCGCTG 
ATCACCTTGT ATAGAAGAAA GTGTCTCTTG AAGCTGAATG AGGTGGCATC ATCTCTGTTT 
GTAAGCTGTG AGGAAGCCCA CGTGGCAGAG GGCATTAGCA TCCTGAATGA GCTGATCATC 
CCCTGTATGC ACCTCATTAA CAACTTTGAC ATCTCCAGAG AAGACATGGA TGCCATTGAG 
GTCATGAGAA ACCGCTGGTG CTCCTATCTG GGACGAGAAG ACATGGACGC AAGCCTGCAG 
ATCAGGCTGG GCGAGCTGCT CCCCCGCCTG CTGGACGGCT CCACGGAGGT GGTGGTGCTG 
AAGGAGCCCC CAAAGATCCG TCCCAACTCC CCATACGACC TCTGCAGCCG CTTTGCAGCG 
GTGATGGAGT CCATCCATGA CGCCTCGACC GTGACTGTGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_025143197.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998965.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa147540
Clone ID Related Accession (Same CDS sequence)	XM_025143197.1
Accession Version	XM_025143197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGCCAA AGGAGGTAGA ACCAATGAAG GCCCAACTGG GAGAAACCAC CTTTACAGTT 
CAGGCAGAGC AACCTCAGGA TGCTAAAGAA GCAGAGCCCA CTGCCCAGAG TAGCTCACAG 
ATCTCGGAAG TGGAAATCCC CAGTGTGGGG AAGGTTCCTG TTAGATCTGA TGCAGATGGA 
TATAATGAGG AGGTGATGCT GAGTCCAGCC ATGCAAGGTG TCATCCTGGC TATTGCAAAA 
GCCCGCCAGA CCTTTGATCG TGATGGGTCT GAAGCGGGGC TCGTTAAGGC ATTCCACGAG 
GAGTACTCCA GACTCTATCT CCTTTCCAAA GAGACCCCGA CCCCTCAGAA CGATGCCCGC 
TTGCAACACG TGCTCGTTTA CTTCCTTCAG AACGATGCTC CCCAGCAGAT TGTGGAACGG 
ACCCTCCTCG AGCAGTTTGC AGACAAAAAC CTCAGCTACG ATGAAAGGTC CATTAGCATC 
ATGAAGGTAG CACGAGATAA ACTGAAAGAG ATCGGTCCTG ATGAGGTCAA TATGGAGGAG 
TATAAGAAGT GGCATGAGGA CTACAGTCTT TTTCGGAAGG TGTCTGTTTA CCTGCTTACA 
GGGTTGGAGC TGTACCAGAA TAGAAAATAC AAGGAGTCCC TGACCTACCT GATCTATGCC 
TACCAAAGCA ACACCACTCT GCTGAAGAAG GGAGCCAACA GAGGAGTGAA TGAATCGCTG 
ATCACCTTGT ATAGAAGAAA GTGTCTCTTG CTGAATGAGG TGGCATCATC TCTGTTTGTA 
AGCTGTGAGG AAGCCCACGT GGCAGAGGGC ATTAGCATCC TGAATGAGCT GATCATCCCC 
TGTATGCACC TCATTAACAA CTTTGACATC TCCAGAGAAG ACATGGATGC CATTGAGGTC 
ATGAGAAACC GCTGGTGCTC CTATCTGGGA CGAGAAGACA TGGACGCAAG CCTGCAGATC 
AGGCTGGGCG AGCTGCTCCC CCGCCTGCTG GACGGCTCCA CGGAGGTGGT GGTGCTGAAG 
GAGCCCCCAA AGATCCGTCC CAACTCCCCA TACGACCTCT GCAGCCGCTT TGCAGCGGTG 
ATGGAGTCCA TCCATGACGC CTCGACCGTG ACTGTGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998965.1
CDS	100..4221
Translation	METQRTRSVHGGTARVVCTGTQHARCAQRYSPYTACTGTQHTCSVHRGRECTGTQHTHGMHGDTGCPEAQHALGFSTHVVCIGTQRSQCAQRHSTHTACTGVQRRQGYSTQRMCRDAVCMGHSTHSMHRGTACMGTACTEMQCTQEHSTHRDTARTQCMLCAQGCHTRRTTACTGLQSSVVSTGMWHTRTELEHTPSLHTDTACTRMWAHSVCTFEPFRSFPQVQQEAWIIVHFAQDCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_025143197.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143197.1

ORF Insert Sequence:

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGCCAA AGGAGGTAGA ACCAATGAAG GCCCAACTGG GAGAAACCAC CTTTACAGTT 
CAGGCAGAGC AACCTCAGGA TGCTAAAGAA GCAGAGCCCA CTGCCCAGAG TAGCTCACAG 
ATCTCGGAAG TGGAAATCCC CAGTGTGGGG AAGGTTCCTG TTAGATCTGA TGCAGATGGA 
TATAATGAGG AGGTGATGCT GAGTCCAGCC ATGCAAGGTG TCATCCTGGC TATTGCAAAA 
GCCCGCCAGA CCTTTGATCG TGATGGGTCT GAAGCGGGGC TCGTTAAGGC ATTCCACGAG 
GAGTACTCCA GACTCTATCT CCTTTCCAAA GAGACCCCGA CCCCTCAGAA CGATGCCCGC 
TTGCAACACG TGCTCGTTTA CTTCCTTCAG AACGATGCTC CCCAGCAGAT TGTGGAACGG 
ACCCTCCTCG AGCAGTTTGC AGACAAAAAC CTCAGCTACG ATGAAAGGTC CATTAGCATC 
ATGAAGGTAG CACGAGATAA ACTGAAAGAG ATCGGTCCTG ATGAGGTCAA TATGGAGGAG 
TATAAGAAGT GGCATGAGGA CTACAGTCTT TTTCGGAAGG TGTCTGTTTA CCTGCTTACA 
GGGTTGGAGC TGTACCAGAA TAGAAAATAC AAGGAGTCCC TGACCTACCT GATCTATGCC 
TACCAAAGCA ACACCACTCT GCTGAAGAAG GGAGCCAACA GAGGAGTGAA TGAATCGCTG 
ATCACCTTGT ATAGAAGAAA GTGTCTCTTG CTGAATGAGG TGGCATCATC TCTGTTTGTA 
AGCTGTGAGG AAGCCCACGT GGCAGAGGGC ATTAGCATCC TGAATGAGCT GATCATCCCC 
TGTATGCACC TCATTAACAA CTTTGACATC TCCAGAGAAG ACATGGATGC CATTGAGGTC 
ATGAGAAACC GCTGGTGCTC CTATCTGGGA CGAGAAGACA TGGACGCAAG CCTGCAGATC 
AGGCTGGGCG AGCTGCTCCC CCGCCTGCTG GACGGCTCCA CGGAGGTGGT GGTGCTGAAG 
GAGCCCCCAA AGATCCGTCC CAACTCCCCA TACGACCTCT GCAGCCGCTT TGCAGCGGTG 
ATGGAGTCCA TCCATGACGC CTCGACCGTG ACTGTGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_025143198.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998966.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

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CloneID	OGa147541
Clone ID Related Accession (Same CDS sequence)	XM_025143198.1
Accession Version	XM_025143198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGATCCAC CTGCAGCCCA CGAGCAGAGC CTGCGCTCTC TGTCCTCGGA GCACGCCATG 
ATTGCAAAGG AGCAGACTGC CAAAGCCATT GCCAACGCAG CCAACGTCTA CGAGAAGAAC 
GGCGTGGAGG CAGCGCTCTG CGAGCCAAAG GAGGTAGAAC CAATGAAGGC CCAACTGGGA 
GAAACCACCT TTACAGTTCA GGCAGAGCAA CCTCAGGATG CTAAAGAAGC AGAGCCCACT 
GCCCAGAGTA GCTCACAGAT CTCGGAAGTG GAAATCCCCA GTGTGGGGAA GGTTCCTGTT 
AGATCTGATG CAGATGGATA TAATGAGGAG GTGATGCTGA GTCCAGCCAT GCAAGGTGTC 
ATCCTGGCTA TTGCAAAAGC CCGCCAGACC TTTGATCGTG ATGGGTCTGA AGCGGGGCTC 
GTTAAGGCAT TCCACGAGGA GTACTCCAGA CTCTATCTCC TTTCCAAAGA GACCCCGACC 
CCTCAGAACG ATGCCCGCTT GCAACACGTG CTCGTTTACT TCCTTCAGAA CGATGCTCCC 
CAGCAGATTG TGGAACGGAC CCTCCTCGAG CAGTTTGCAG ACAAAAACCT CAGCTACGAT 
GAAAGGTCCA TTAGCATCAT GAAGGTAGCA CGAGATAAAC TGAAAGAGAT CGGTCCTGAT 
GAGGTCAATA TGGAGGAGTA TAAGAAGTGG CATGAGGACT ACAGTCTTTT TCGGAAGGTG 
TCTGTTTACC TGCTTACAGG GTTGGAGCTG TACCAGAATA GAAAATACAA GGAGTCCCTG 
ACCTACCTGA TCTATGCCTA CCAAAGCAAC ACCACTCTGC TGAAGAAGGG AGCCAACAGA 
GGAGTGAATG AATCGCTGAT CACCTTGTAT AGAAGAAAGT GTCTCTTGAA GCTGAATGAG 
GTGGCATCAT CTCTGTTTGT AAGCTGTGAG GAAGCCCACG TGGCAGAGGG CATTAGCATC 
CTGAATGAGC TGATCATCCC CTGTATGCAC CTCATTAACA ACTTTGACAT CTCCAGAGAA 
GACATGGATG CCATTGAGGT CATGAGAAAC CGCTGGTGCT CCTATCTGGG ACGAGAAGAC 
ATGGACGCAA GCCTGCAGAT CAGGCTGGGC GAGCTGCTCC CCCGCCTGCT GGACGGCTCC 
ACGGAGGTGG TGGTGCTGAA GGAGCCCCCA AAGATCCGTC CCAACTCCCC ATACGACCTC 
TGCAGCCGCT TTGCAGCGGT GATGGAGTCC ATCCATGACG CCTCGACCGT GACTGTGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998966.1
CDS	95..4177
Translation	METQRTRSVHGGTARVVCTGTQHARCAQRYSPYTACTGTQHTCSVHRGRECTGTQHTHGMHGDTGCPEAQHALGFSTHVVCIGTQRSQCAQRHSTHTACTGVQRRQGYSTQRMCRDAVCMGHSTHSMHRGTACMGTACTEMQCTQEHSTHRDTARTQCMLCAQGCHTRRTTACTGLQSSVVSTGMWHTRTELEHTPSLHTDTACTRMWAHSVCTFEPFRSFPQVQQEAWIIVHFAQDCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_025143198.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143198.1

ORF Insert Sequence:

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGATCCAC CTGCAGCCCA CGAGCAGAGC CTGCGCTCTC TGTCCTCGGA GCACGCCATG 
ATTGCAAAGG AGCAGACTGC CAAAGCCATT GCCAACGCAG CCAACGTCTA CGAGAAGAAC 
GGCGTGGAGG CAGCGCTCTG CGAGCCAAAG GAGGTAGAAC CAATGAAGGC CCAACTGGGA 
GAAACCACCT TTACAGTTCA GGCAGAGCAA CCTCAGGATG CTAAAGAAGC AGAGCCCACT 
GCCCAGAGTA GCTCACAGAT CTCGGAAGTG GAAATCCCCA GTGTGGGGAA GGTTCCTGTT 
AGATCTGATG CAGATGGATA TAATGAGGAG GTGATGCTGA GTCCAGCCAT GCAAGGTGTC 
ATCCTGGCTA TTGCAAAAGC CCGCCAGACC TTTGATCGTG ATGGGTCTGA AGCGGGGCTC 
GTTAAGGCAT TCCACGAGGA GTACTCCAGA CTCTATCTCC TTTCCAAAGA GACCCCGACC 
CCTCAGAACG ATGCCCGCTT GCAACACGTG CTCGTTTACT TCCTTCAGAA CGATGCTCCC 
CAGCAGATTG TGGAACGGAC CCTCCTCGAG CAGTTTGCAG ACAAAAACCT CAGCTACGAT 
GAAAGGTCCA TTAGCATCAT GAAGGTAGCA CGAGATAAAC TGAAAGAGAT CGGTCCTGAT 
GAGGTCAATA TGGAGGAGTA TAAGAAGTGG CATGAGGACT ACAGTCTTTT TCGGAAGGTG 
TCTGTTTACC TGCTTACAGG GTTGGAGCTG TACCAGAATA GAAAATACAA GGAGTCCCTG 
ACCTACCTGA TCTATGCCTA CCAAAGCAAC ACCACTCTGC TGAAGAAGGG AGCCAACAGA 
GGAGTGAATG AATCGCTGAT CACCTTGTAT AGAAGAAAGT GTCTCTTGAA GCTGAATGAG 
GTGGCATCAT CTCTGTTTGT AAGCTGTGAG GAAGCCCACG TGGCAGAGGG CATTAGCATC 
CTGAATGAGC TGATCATCCC CTGTATGCAC CTCATTAACA ACTTTGACAT CTCCAGAGAA 
GACATGGATG CCATTGAGGT CATGAGAAAC CGCTGGTGCT CCTATCTGGG ACGAGAAGAC 
ATGGACGCAA GCCTGCAGAT CAGGCTGGGC GAGCTGCTCC CCCGCCTGCT GGACGGCTCC 
ACGGAGGTGG TGGTGCTGAA GGAGCCCCCA AAGATCCGTC CCAACTCCCC ATACGACCTC 
TGCAGCCGCT TTGCAGCGGT GATGGAGTCC ATCCATGACG CCTCGACCGT GACTGTGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_025143199.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998967.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

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CloneID	OGa147542
Clone ID Related Accession (Same CDS sequence)	XM_025143199.1
Accession Version	XM_025143199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGCCAA AGGAGGTAGA ACCAATGAAG GCCCAACTGG GAGAAACCAC CTTTACAGTT 
CAGGCAGAGC AACCTCAGGA TGCTAAAGAA GCAGAGCCCA CTGCCCAGAG TAGCTCACAG 
ATCTCGGAAG TGGAAATCCC CAGTGTGGGG AAGGTTCCTG TTAGATCTGA TGCAGATGGA 
TATAATGAGG AGGCATTCCA CGAGGAGTAC TCCAGACTCT ATCTCCTTTC CAAAGAGACC 
CCGACCCCTC AGAACGATGC CCGCTTGCAA CACGTGCTCG TTTACTTCCT TCAGAACGAT 
GCTCCCCAGC AGATTGTGGA ACGGACCCTC CTCGAGCAGT TTGCAGACAA AAACCTCAGC 
TACGATGAAA GGTCCATTAG CATCATGAAG GTAGCACGAG ATAAACTGAA AGAGATCGGT 
CCTGATGAGG TCAATATGGA GGAGTATAAG AAGTGGCATG AGGACTACAG TCTTTTTCGG 
AAGGTGTCTG TTTACCTGCT TACAGGGTTG GAGCTGTACC AGAATAGAAA ATACAAGGAG 
TCCCTGACCT ACCTGATCTA TGCCTACCAA AGCAACACCA CTCTGCTGAA GAAGGGAGCC 
AACAGAGGAG TGAATGAATC GCTGATCACC TTGTATAGAA GAAAGTGTCT CTTGAAGCTG 
AATGAGGTGG CATCATCTCT GTTTGTAAGC TGTGAGGAAG CCCACGTGGC AGAGGGCATT 
AGCATCCTGA ATGAGCTGAT CATCCCCTGT ATGCACCTCA TTAACAACTT TGACATCTCC 
AGAGAAGACA TGGATGCCAT TGAGGTCATG AGAAACCGCT GGTGCTCCTA TCTGGGACGA 
GAAGACATGG ACGCAAGCCT GCAGATCAGG CTGGGCGAGC TGCTCCCCCG CCTGCTGGAC 
GGCTCCACGG AGGTGGTGGT GCTGAAGGAG CCCCCAAAGA TCCGTCCCAA CTCCCCATAC 
GACCTCTGCA GCCGCTTTGC AGCGGTGATG GAGTCCATCC ATGACGCCTC GACCGTGACT 
GTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998967.1
CDS	98..4126
Translation	METQRTRSVHGGTARVVCTGTQHARCAQRYSPYTACTGTQHTCSVHRGRECTGTQHTHGMHGDTGCPEAQHALGFSTHVVCIGTQRSQCAQRHSTHTACTGVQRRQGYSTQRMCRDAVCMGHSTHSMHRGTACMGTACTEMQCTQEHSTHRDTARTQCMLCAQGCHTRRTTACTGLQSSVVSTGMWHTRTELEHTPSLHTDTACTRMWAHSVCTFEPFRSFPQVQQEAWIIVHFAQDCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_025143199.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143199.1

ORF Insert Sequence:

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGCCAA AGGAGGTAGA ACCAATGAAG GCCCAACTGG GAGAAACCAC CTTTACAGTT 
CAGGCAGAGC AACCTCAGGA TGCTAAAGAA GCAGAGCCCA CTGCCCAGAG TAGCTCACAG 
ATCTCGGAAG TGGAAATCCC CAGTGTGGGG AAGGTTCCTG TTAGATCTGA TGCAGATGGA 
TATAATGAGG AGGCATTCCA CGAGGAGTAC TCCAGACTCT ATCTCCTTTC CAAAGAGACC 
CCGACCCCTC AGAACGATGC CCGCTTGCAA CACGTGCTCG TTTACTTCCT TCAGAACGAT 
GCTCCCCAGC AGATTGTGGA ACGGACCCTC CTCGAGCAGT TTGCAGACAA AAACCTCAGC 
TACGATGAAA GGTCCATTAG CATCATGAAG GTAGCACGAG ATAAACTGAA AGAGATCGGT 
CCTGATGAGG TCAATATGGA GGAGTATAAG AAGTGGCATG AGGACTACAG TCTTTTTCGG 
AAGGTGTCTG TTTACCTGCT TACAGGGTTG GAGCTGTACC AGAATAGAAA ATACAAGGAG 
TCCCTGACCT ACCTGATCTA TGCCTACCAA AGCAACACCA CTCTGCTGAA GAAGGGAGCC 
AACAGAGGAG TGAATGAATC GCTGATCACC TTGTATAGAA GAAAGTGTCT CTTGAAGCTG 
AATGAGGTGG CATCATCTCT GTTTGTAAGC TGTGAGGAAG CCCACGTGGC AGAGGGCATT 
AGCATCCTGA ATGAGCTGAT CATCCCCTGT ATGCACCTCA TTAACAACTT TGACATCTCC 
AGAGAAGACA TGGATGCCAT TGAGGTCATG AGAAACCGCT GGTGCTCCTA TCTGGGACGA 
GAAGACATGG ACGCAAGCCT GCAGATCAGG CTGGGCGAGC TGCTCCCCCG CCTGCTGGAC 
GGCTCCACGG AGGTGGTGGT GCTGAAGGAG CCCCCAAAGA TCCGTCCCAA CTCCCCATAC 
GACCTCTGCA GCCGCTTTGC AGCGGTGATG GAGTCCATCC ATGACGCCTC GACCGTGACT 
GTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_025143200.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998968.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa147543
Clone ID Related Accession (Same CDS sequence)	XM_025143200.1
Accession Version	XM_025143200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGGTGA TGCTGAGTCC AGCCATGCAA GGTGTCATCC TGGCTATTGC AAAAGCCCGC 
CAGACCTTTG ATCGTGATGG GTCTGAAGCG GGGCTCGTTA AGGCATTCCA CGAGGAGTAC 
TCCAGACTCT ATCTCCTTTC CAAAGAGACC CCGACCCCTC AGAACGATGC CCGCTTGCAA 
CACGTGCTCG TTTACTTCCT TCAGAACGAT GCTCCCCAGC AGATTGTGGA ACGGACCCTC 
CTCGAGCAGT TTGCAGACAA AAACCTCAGC TACGATGAAA GGTCCATTAG CATCATGAAG 
GTAGCACGAG ATAAACTGAA AGAGATCGGT CCTGATGAGG TCAATATGGA GGAGTATAAG 
AAGTGGCATG AGGACTACAG TCTTTTTCGG AAGGTGTCTG TTTACCTGCT TACAGGGTTG 
GAGCTGTACC AGAATAGAAA ATACAAGGAG TCCCTGACCT ACCTGATCTA TGCCTACCAA 
AGCAACACCA CTCTGCTGAA GAAGGGAGCC AACAGAGGAG TGAATGAATC GCTGATCACC 
TTGTATAGAA GAAAGTGTCT CTTGAAGCTG AATGAGGTGG CATCATCTCT GTTTGTAAGC 
TGTGAGGAAG CCCACGTGGC AGAGGGCATT AGCATCCTGA ATGAGCTGAT CATCCCCTGT 
ATGCACCTCA TTAACAACTT TGACATCTCC AGAGAAGACA TGGATGCCAT TGAGGTCATG 
AGAAACCGCT GGTGCTCCTA TCTGGGACGA GAAGACATGG ACGCAAGCCT GCAGATCAGG 
CTGGGCGAGC TGCTCCCCCG CCTGCTGGAC GGCTCCACGG AGGTGGTGGT GCTGAAGGAG 
CCCCCAAAGA TCCGTCCCAA CTCCCCATAC GACCTCTGCA GCCGCTTTGC AGCGGTGATG 
GAGTCCATCC ATGACGCCTC GACCGTGACT GTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998968.1
CDS	97..4035
Translation	METQRTRSVHGGTARVVCTGTQHARCAQRYSPYTACTGTQHTCSVHRGRECTGTQHTHGMHGDTGCPEAQHALGFSTHVVCIGTQRSQCAQRHSTHTACTGVQRRQGYSTQRMCRDAVCMGHSTHSMHRGTACMGTACTEMQCTQEHSTHRDTARTQCMLCAQGCHTRRTTACTGLQSSVVSTGMWHTRTELEHTPSLHTDTACTRMWAHSVCTFEPFRSFPQVQQEAWIIVHFAQDCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_025143200.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143200.1

ORF Insert Sequence:

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGGTGA TGCTGAGTCC AGCCATGCAA GGTGTCATCC TGGCTATTGC AAAAGCCCGC 
CAGACCTTTG ATCGTGATGG GTCTGAAGCG GGGCTCGTTA AGGCATTCCA CGAGGAGTAC 
TCCAGACTCT ATCTCCTTTC CAAAGAGACC CCGACCCCTC AGAACGATGC CCGCTTGCAA 
CACGTGCTCG TTTACTTCCT TCAGAACGAT GCTCCCCAGC AGATTGTGGA ACGGACCCTC 
CTCGAGCAGT TTGCAGACAA AAACCTCAGC TACGATGAAA GGTCCATTAG CATCATGAAG 
GTAGCACGAG ATAAACTGAA AGAGATCGGT CCTGATGAGG TCAATATGGA GGAGTATAAG 
AAGTGGCATG AGGACTACAG TCTTTTTCGG AAGGTGTCTG TTTACCTGCT TACAGGGTTG 
GAGCTGTACC AGAATAGAAA ATACAAGGAG TCCCTGACCT ACCTGATCTA TGCCTACCAA 
AGCAACACCA CTCTGCTGAA GAAGGGAGCC AACAGAGGAG TGAATGAATC GCTGATCACC 
TTGTATAGAA GAAAGTGTCT CTTGAAGCTG AATGAGGTGG CATCATCTCT GTTTGTAAGC 
TGTGAGGAAG CCCACGTGGC AGAGGGCATT AGCATCCTGA ATGAGCTGAT CATCCCCTGT 
ATGCACCTCA TTAACAACTT TGACATCTCC AGAGAAGACA TGGATGCCAT TGAGGTCATG 
AGAAACCGCT GGTGCTCCTA TCTGGGACGA GAAGACATGG ACGCAAGCCT GCAGATCAGG 
CTGGGCGAGC TGCTCCCCCG CCTGCTGGAC GGCTCCACGG AGGTGGTGGT GCTGAAGGAG 
CCCCCAAAGA TCCGTCCCAA CTCCCCATAC GACCTCTGCA GCCGCTTTGC AGCGGTGATG 
GAGTCCATCC ATGACGCCTC GACCGTGACT GTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_025143201.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998969.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa147544
Clone ID Related Accession (Same CDS sequence)	XM_025143201.1
Accession Version	XM_025143201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGATCCAC CTGCAGCCCA CGAGCAGAGC CTGCGCTCTC TGTCCTCGGA GCACGCCATG 
ATTGCAAAGG AGCAGACTGC CAAAGCCATT GCCAACGCAG CCAACGTCTA CGAGAAGAAC 
GGCGTGGAGG CAGCGCTCTG CGAGGTGATG CTGAGTCCAG CCATGCAAGG TGTCATCCTG 
GCTATTGCAA AAGCCCGCCA GACCTTTGAT CGTGATGGGT CTGAAGCGGG GCTCGTTAAG 
GCATTCCACG AGGAGTACTC CAGACTCTAT CTCCTTTCCA AAGAGACCCC GACCCCTCAG 
AACGATGCCC GCTTGCAACA CGTGCTCGTT TACTTCCTTC AGAACGATGC TCCCCAGCAG 
ATTGTGGAAC GGACCCTCCT CGAGCAGTTT GCAGACAAAA ACCTCAGCTA CGATGAAAGG 
TCCATTAGCA TCATGAAGGT AGCACGAGAT AAACTGAAAG AGATCGGTCC TGATGAGGTC 
AATATGGAGG AGTATAAGAA GTGGCATGAG GACTACAGTC TTTTTCGGAA GGTGTCTGTT 
TACCTGCTTA CAGGGTTGGA GCTGTACCAG AATAGAAAAT ACAAGGAGTC CCTGACCTAC 
CTGATCTATG CCTACCAAAG CAACACCACT CTGCTGAAGA AGGGAGCCAA CAGAGGAGTG 
AATGAATCGC TGATCACCTT GTATAGAAGA AAGTGTCTCT TGAAGCTGAA TGAGGTGGCA 
TCATCTCTGT TTGTAAGCTG TGAGGAAGCC CACGTGGCAG AGGGCATTAG CATCCTGAAT 
GAGCTGATCA TCCCCTGTAT GCACCTCATT AACAACTTTG ACATCTCCAG AGAAGACATG 
GATGCCATTG AGGTCATGAG AAACCGCTGG TGCTCCTATC TGGGACGAGA AGACATGGAC 
GCAAGCCTGC AGATCAGGCT GGGCGAGCTG CTCCCCCGCC TGCTGGACGG CTCCACGGAG 
GTGGTGGTGC TGAAGGAGCC CCCAAAGATC CGTCCCAACT CCCCATACGA CCTCTGCAGC 
CGCTTTGCAG CGGTGATGGA GTCCATCCAT GACGCCTCGA CCGTGACTGT GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998969.1
CDS	95..3991
Translation	METQRTRSVHGGTARVVCTGTQHARCAQRYSPYTACTGTQHTCSVHRGRECTGTQHTHGMHGDTGCPEAQHALGFSTHVVCIGTQRSQCAQRHSTHTACTGVQRRQGYSTQRMCRDAVCMGHSTHSMHRGTACMGTACTEMQCTQEHSTHRDTARTQCMLCAQGCHTRRTTACTGLQSSVVSTGMWHTRTELEHTPSLHTDTACTRMWAHSVCTFEPFRSFPQVQQEAWIIVHFAQDCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_025143201.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143201.1

ORF Insert Sequence:

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGATCCAC CTGCAGCCCA CGAGCAGAGC CTGCGCTCTC TGTCCTCGGA GCACGCCATG 
ATTGCAAAGG AGCAGACTGC CAAAGCCATT GCCAACGCAG CCAACGTCTA CGAGAAGAAC 
GGCGTGGAGG CAGCGCTCTG CGAGGTGATG CTGAGTCCAG CCATGCAAGG TGTCATCCTG 
GCTATTGCAA AAGCCCGCCA GACCTTTGAT CGTGATGGGT CTGAAGCGGG GCTCGTTAAG 
GCATTCCACG AGGAGTACTC CAGACTCTAT CTCCTTTCCA AAGAGACCCC GACCCCTCAG 
AACGATGCCC GCTTGCAACA CGTGCTCGTT TACTTCCTTC AGAACGATGC TCCCCAGCAG 
ATTGTGGAAC GGACCCTCCT CGAGCAGTTT GCAGACAAAA ACCTCAGCTA CGATGAAAGG 
TCCATTAGCA TCATGAAGGT AGCACGAGAT AAACTGAAAG AGATCGGTCC TGATGAGGTC 
AATATGGAGG AGTATAAGAA GTGGCATGAG GACTACAGTC TTTTTCGGAA GGTGTCTGTT 
TACCTGCTTA CAGGGTTGGA GCTGTACCAG AATAGAAAAT ACAAGGAGTC CCTGACCTAC 
CTGATCTATG CCTACCAAAG CAACACCACT CTGCTGAAGA AGGGAGCCAA CAGAGGAGTG 
AATGAATCGC TGATCACCTT GTATAGAAGA AAGTGTCTCT TGAAGCTGAA TGAGGTGGCA 
TCATCTCTGT TTGTAAGCTG TGAGGAAGCC CACGTGGCAG AGGGCATTAG CATCCTGAAT 
GAGCTGATCA TCCCCTGTAT GCACCTCATT AACAACTTTG ACATCTCCAG AGAAGACATG 
GATGCCATTG AGGTCATGAG AAACCGCTGG TGCTCCTATC TGGGACGAGA AGACATGGAC 
GCAAGCCTGC AGATCAGGCT GGGCGAGCTG CTCCCCCGCC TGCTGGACGG CTCCACGGAG 
GTGGTGGTGC TGAAGGAGCC CCCAAAGATC CGTCCCAACT CCCCATACGA CCTCTGCAGC 
CGCTTTGCAG CGGTGATGGA GTCCATCCAT GACGCCTCGA CCGTGACTGT GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_025143202.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998970.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

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CloneID	OGa147545
Clone ID Related Accession (Same CDS sequence)	XM_025143202.1
Accession Version	XM_025143202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGGCAT TCCACGAGGA GTACTCCAGA CTCTATCTCC TTTCCAAAGA GACCCCGACC 
CCTCAGAACG ATGCCCGCTT GCAACACGTG CTCGTTTACT TCCTTCAGAA CGATGCTCCC 
CAGCAGATTG TGGAACGGAC CCTCCTCGAG CAGTTTGCAG ACAAAAACCT CAGCTACGAT 
GAAAGGTCCA TTAGCATCAT GAAGGTAGCA CGAGATAAAC TGAAAGAGAT CGGTCCTGAT 
GAGGTCAATA TGGAGGAGTA TAAGAAGTGG CATGAGGACT ACAGTCTTTT TCGGAAGGTG 
TCTGTTTACC TGCTTACAGG GTTGGAGCTG TACCAGAATA GAAAATACAA GGAGTCCCTG 
ACCTACCTGA TCTATGCCTA CCAAAGCAAC ACCACTCTGC TGAAGAAGGG AGCCAACAGA 
GGAGTGAATG AATCGCTGAT CACCTTGTAT AGAAGAAAGT GTCTCTTGAA GCTGAATGAG 
GTGGCATCAT CTCTGTTTGT AAGCTGTGAG GAAGCCCACG TGGCAGAGGG CATTAGCATC 
CTGAATGAGC TGATCATCCC CTGTATGCAC CTCATTAACA ACTTTGACAT CTCCAGAGAA 
GACATGGATG CCATTGAGGT CATGAGAAAC CGCTGGTGCT CCTATCTGGG ACGAGAAGAC 
ATGGACGCAA GCCTGCAGAT CAGGCTGGGC GAGCTGCTCC CCCGCCTGCT GGACGGCTCC 
ACGGAGGTGG TGGTGCTGAA GGAGCCCCCA AAGATCCGTC CCAACTCCCC ATACGACCTC 
TGCAGCCGCT TTGCAGCGGT GATGGAGTCC ATCCATGACG CCTCGACCGT GACTGTGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998970.1
CDS	95..3937
Translation	METQRTRSVHGGTARVVCTGTQHARCAQRYSPYTACTGTQHTCSVHRGRECTGTQHTHGMHGDTGCPEAQHALGFSTHVVCIGTQRSQCAQRHSTHTACTGVQRRQGYSTQRMCRDAVCMGHSTHSMHRGTACMGTACTEMQCTQEHSTHRDTARTQCMLCAQGCHTRRTTACTGLQSSVVSTGMWHTRTELEHTPSLHTDTACTRMWAHSVCTFEPFRSFPQVQQEAWIIVHFAQDCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_025143202.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143202.1

ORF Insert Sequence:

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGGTGATG CCACATCTGA TTTTGCAAGC TGTGTTGAAA TGAATGATCC ACCTGCAGCC 
CACGAGCAGA GCCTGCGCTC TCTGTCCTCG GAGCACGCCA TGATTGCAAA GGAGCAGACT 
GCCAAAGCCA TTGCCAACGC AGCCAACGTC TACGAGAAGA ACGGCGTGGA GGCAGCGCTC 
TGCGAGGCAT TCCACGAGGA GTACTCCAGA CTCTATCTCC TTTCCAAAGA GACCCCGACC 
CCTCAGAACG ATGCCCGCTT GCAACACGTG CTCGTTTACT TCCTTCAGAA CGATGCTCCC 
CAGCAGATTG TGGAACGGAC CCTCCTCGAG CAGTTTGCAG ACAAAAACCT CAGCTACGAT 
GAAAGGTCCA TTAGCATCAT GAAGGTAGCA CGAGATAAAC TGAAAGAGAT CGGTCCTGAT 
GAGGTCAATA TGGAGGAGTA TAAGAAGTGG CATGAGGACT ACAGTCTTTT TCGGAAGGTG 
TCTGTTTACC TGCTTACAGG GTTGGAGCTG TACCAGAATA GAAAATACAA GGAGTCCCTG 
ACCTACCTGA TCTATGCCTA CCAAAGCAAC ACCACTCTGC TGAAGAAGGG AGCCAACAGA 
GGAGTGAATG AATCGCTGAT CACCTTGTAT AGAAGAAAGT GTCTCTTGAA GCTGAATGAG 
GTGGCATCAT CTCTGTTTGT AAGCTGTGAG GAAGCCCACG TGGCAGAGGG CATTAGCATC 
CTGAATGAGC TGATCATCCC CTGTATGCAC CTCATTAACA ACTTTGACAT CTCCAGAGAA 
GACATGGATG CCATTGAGGT CATGAGAAAC CGCTGGTGCT CCTATCTGGG ACGAGAAGAC 
ATGGACGCAA GCCTGCAGAT CAGGCTGGGC GAGCTGCTCC CCCGCCTGCT GGACGGCTCC 
ACGGAGGTGG TGGTGCTGAA GGAGCCCCCA AAGATCCGTC CCAACTCCCC ATACGACCTC 
TGCAGCCGCT TTGCAGCGGT GATGGAGTCC ATCCATGACG CCTCGACCGT GACTGTGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_025143203.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998971.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa147546
Clone ID Related Accession (Same CDS sequence)	XM_025143203.1
Accession Version	XM_025143203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGATCCAC CTGCAGCCCA CGAGCAGAGC CTGCGCTCTC TGTCCTCGGA GCACGCCATG 
ATTGCAAAGG AGCAGACTGC CAAAGCCATT GCCAACGCAG CCAACGTCTA CGAGAAGAAC 
GGCGTGGAGG CAGCGCTCTG CGAGGCATTC CACGAGGAGT ACTCCAGACT CTATCTCCTT 
TCCAAAGAGA CCCCGACCCC TCAGAACGAT GCCCGCTTGC AACACGTGCT CGTTTACTTC 
CTTCAGAACG ATGCTCCCCA GCAGATTGTG GAACGGACCC TCCTCGAGCA GTTTGCAGAC 
AAAAACCTCA GCTACGATGA AAGGTCCATT AGCATCATGA AGGTAGCACG AGATAAACTG 
AAAGAGATCG GTCCTGATGA GGTCAATATG GAGGAGTATA AGAAGTGGCA TGAGGACTAC 
AGTCTTTTTC GGAAGGTGTC TGTTTACCTG CTTACAGGGT TGGAGCTGTA CCAGAATAGA 
AAATACAAGG AGTCCCTGAC CTACCTGATC TATGCCTACC AAAGCAACAC CACTCTGCTG 
AAGAAGGGAG CCAACAGAGG AGTGAATGAA TCGCTGATCA CCTTGTATAG AAGAAAGTGT 
CTCTTGAAGC TGAATGAGGT GGCATCATCT CTGTTTGTAA GCTGTGAGGA AGCCCACGTG 
GCAGAGGGCA TTAGCATCCT GAATGAGCTG ATCATCCCCT GTATGCACCT CATTAACAAC 
TTTGACATCT CCAGAGAAGA CATGGATGCC ATTGAGGTCA TGAGAAACCG CTGGTGCTCC 
TATCTGGGAC GAGAAGACAT GGACGCAAGC CTGCAGATCA GGCTGGGCGA GCTGCTCCCC 
CGCCTGCTGG ACGGCTCCAC GGAGGTGGTG GTGCTGAAGG AGCCCCCAAA GATCCGTCCC 
AACTCCCCAT ACGACCTCTG CAGCCGCTTT GCAGCGGTGA TGGAGTCCAT CCATGACGCC 
TCGACCGTGA CTGTGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998971.1
CDS	93..3893
Translation	METQRTRSVHGGTARVVCTGTQHARCAQRYSPYTACTGTQHTCSVHRGRECTGTQHTHGMHGDTGCPEAQHALGFSTHVVCIGTQRSQCAQRHSTHTACTGVQRRQGYSTQRMCRDAVCMGHSTHSMHRGTACMGTACTEMQCTQEHSTHRDTARTQCMLCAQGCHTRRTTACTGLQSSVVSTGMWHTRTELEHTPSLHTDTACTRMWAHSVCTFEPFRSFPQVQQEAWIIVHFAQDCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_025143203.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143203.1

ORF Insert Sequence:

ATGGAGACAC AGCGCACGCG CAGCGTGCAT GGGGGCACAG CGCGTGTAGT GTGCACGGGG 
ACACAGCACG CACGGTGTGC ACAGAGATAC AGCCCGTACA CAGCTTGCAC AGGGACACAG 
CACACGTGCA GCGTGCACAG GGGTAGAGAA TGCACGGGGA CACAGCACAC ACACGGCATG 
CACGGGGATA CAGGGTGCCC TGAGGCACAG CATGCACTGG GATTCAGCAC CCATGTAGTG 
TGCATAGGGA CACAGCGCTC ACAGTGTGCA CAAAGACACA GCACACACAC AGCTTGCACT 
GGGGTGCAGC GTAGACAGGG GTACAGTACA CAGAGGATGT GCAGAGATGC AGTGTGCATG 
GGACACAGCA CACACAGCAT GCACAGGGGC ACAGCATGCA TGGGGACAGC ATGCACTGAG 
ATGCAGTGCA CCCAGGAGCA CAGCACGCAC AGGGACACAG CAAGGACACA GTGCATGCTC 
TGTGCACAGG GATGCCACAC ACGCAGAACC ACAGCATGCA CAGGGCTGCA GAGCTCTGTG 
GTGAGCACAG GGATGTGGCA TACACGCACA GAGCTAGAGC ACACACCCAG CTTGCACACG 
GACACAGCGT GCACGAGGAT GTGGGCTCAT TCTGTGTGCA CATTTGAGCC CTTCAGGAGC 
TTTCCTCAGG TGCAGCAGGA GGCATGGATC ATTGTGCACT TTGCACAGGA TTGCCAGGTT 
TTAAACAAGA TGAAAGAGAT AACAGGAATT CAGGATGCAG ATTTCCTTCA CGCTGCTCTC 
AAGGCTGCTA AGGGAAACCT GATGGAGGCA CTCATCGTCC TCACAGAGGA GCGTGATCAG 
GAGCCTGTTC AGAACACAGC TGCTGCAGAG CCGTCCTCTT GGGAAGGCAG TGCAGTGGGC 
AAAGAGCCCC CACAAGGTGG TGCTGCGTTC GACCCTGAAA AGAAAGGAGA TGTGCACTCA 
GCTGTTGCCT ACGGACAGCT GGAATCACCA AAAGCTCATG CTGCTGAAAG ACCACAGGAG 
GTCCATTCTC CTGAACACAA AAACCGCTCC AAAAGGAAAC GCTGTGAAGT CTGGGGAGAG 
AATTCCAAAC AGAGCGACTG GAAGAGAGTG GGCGATTGGC CTGTGGGGAT GAAGAACATC 
GGCAACACGT GTTGGTTTAG TGCTGTCATA CAGTCTCTTT TCCAGTTGCC GCAGTTCCGG 
AGGTTGGTGC TCAGCTACTC CTTCCCACCA AACGTACTTG AGAGCTGCCG GACGCGCACT 
GGAAAAAGAA ATATTGCATT CATGCAAGAG CTTCAGTGTC TGTTTGCTTT AATGCTGGGA 
ACACGTCGGA AGTTTGTGGA CCCTTCTGCA GCACTGGAAC TCTTAAGGGA TGCGTTTAAA 
TCAACCGAGG AGCAGCAGCA AGATGTGAGT GAGTTCACAC ACAAACTGCT GGATTGGCTG 
GAAGATGCAT TCCAGCTCGC TGTGAATGTC AGAAGCCCTG GGGACAAATC CGAAAACCCA 
ATGGTCCAAC TTTTCTATGG GACTTTCTTG ACTGAGGGGG TCCACGAGGG CAATACTTTT 
TCCAAGATCG AAACCTTTGG TCAGTATCCC CTTCAGGTAA ATGGTTATCG AAACTTGAAT 
GAGTGCTTGG AAGGAGCCAT GGTGGAGGGA GAGATGGATG AGGAGACAGC AACTCAGTCA 
GTGAAATATG TACAGGAGCG CTGGTTCACC AAACTCCCTC CTGTGCTGAC CTTTGAACTC 
TCCCGATTTG AGTTCAACCA ATCCCTGGGA CAGCCAGAGA AAATCCACAC AAAGCTGGAG 
TTCCCTCAGA CTATTTACAT GGACAGGTAC CTCTACTGCA GCAAAGAGCT TATTCAGACA 
AAGAGAGAAG AAATGAAGAA ATTGAAGGAG AAGATGCTGG TTCTGCAACA GAAACTGGAA 
AGGTATATGA AGTATGGCTC TGGCCCAGCA CGCTTCCCAC TGCCTGACAT GCTGCAATAT 
GTTCTGGAAT TCATCACTAC AAAGCCTGCT GGAGCTGTGT CCTCTGCGTG CGTGTCTTCT 
ACTGAGGACT CTCAGATGAT GGACAGGCAG TCCCAGGGCG AGTCGCTCAT TCTGGGCACG 
CCTTCACAGC CTGACAGCAT GCTAGATGGA AAGGATGGTA AGCCTGAAGA TGAAGCTGTC 
CTATTGGCAA ATTCCTCACC TCAGCAACAA CTGAACGCGC CTCTGCAGCC TTCTGAACCA 
CCAGCAGAGA TGTCAGACTG TCCTGCTCCC CACGTGGTTT CTGAGGAAGA GATGAACCTG 
GTTACGACGT GTCTGCAGCG ATGGAGGAAT GAGATTGAGC AAGATGTGCG AGATCTGAAG 
GAGTCCATCG CCAGAATCAG CCTATCCATT GACGAAATGT ACAGCGACCC TCACCTCCAG 
CAGGTTCCTT ATCACTTGCA CGCTGTCTTG GTGCATGAAG GGCAAGCAAA TGCTGGTCAC 
TACTGGGCCT TCATATATGA CCAGCCTCGG AAAAGCTGGC TGAAATACAA TGACATCTCA 
GTGACAGAAT CATCGTGGGA AGAGCTGGAG AGAGATTCAT TTGGTGGCCT GAGGAACGCC 
AGTGCTTACT GCCTGATGTA CATAAGCGAT AAGGTGTCCC ACGTTGTTGC AGGTGAAGGT 
GACGGCTCAG AGGTTGGGCA GTTCCAGAAG GAGGTGGAGG CTTTGCCCCC AGAGCTGAGG 
CGTTACATCC AGGAGGACAA CTGGCGGCTC GAGCAGGAGG CAGAAGAGTG GGAGGAGGAG 
CAGTCCTGCA AGATTCCTTC AACTGCTTCT GAATCTCAAG AGCTCTCTCC TGAGTCGGGG 
CTAGATCCAC CTGCAGCCCA CGAGCAGAGC CTGCGCTCTC TGTCCTCGGA GCACGCCATG 
ATTGCAAAGG AGCAGACTGC CAAAGCCATT GCCAACGCAG CCAACGTCTA CGAGAAGAAC 
GGCGTGGAGG CAGCGCTCTG CGAGGCATTC CACGAGGAGT ACTCCAGACT CTATCTCCTT 
TCCAAAGAGA CCCCGACCCC TCAGAACGAT GCCCGCTTGC AACACGTGCT CGTTTACTTC 
CTTCAGAACG ATGCTCCCCA GCAGATTGTG GAACGGACCC TCCTCGAGCA GTTTGCAGAC 
AAAAACCTCA GCTACGATGA AAGGTCCATT AGCATCATGA AGGTAGCACG AGATAAACTG 
AAAGAGATCG GTCCTGATGA GGTCAATATG GAGGAGTATA AGAAGTGGCA TGAGGACTAC 
AGTCTTTTTC GGAAGGTGTC TGTTTACCTG CTTACAGGGT TGGAGCTGTA CCAGAATAGA 
AAATACAAGG AGTCCCTGAC CTACCTGATC TATGCCTACC AAAGCAACAC CACTCTGCTG 
AAGAAGGGAG CCAACAGAGG AGTGAATGAA TCGCTGATCA CCTTGTATAG AAGAAAGTGT 
CTCTTGAAGC TGAATGAGGT GGCATCATCT CTGTTTGTAA GCTGTGAGGA AGCCCACGTG 
GCAGAGGGCA TTAGCATCCT GAATGAGCTG ATCATCCCCT GTATGCACCT CATTAACAAC 
TTTGACATCT CCAGAGAAGA CATGGATGCC ATTGAGGTCA TGAGAAACCG CTGGTGCTCC 
TATCTGGGAC GAGAAGACAT GGACGCAAGC CTGCAGATCA GGCTGGGCGA GCTGCTCCCC 
CGCCTGCTGG ACGGCTCCAC GGAGGTGGTG GTGCTGAAGG AGCCCCCAAA GATCCGTCCC 
AACTCCCCAT ACGACCTCTG CAGCCGCTTT GCAGCGGTGA TGGAGTCCAT CCATGACGCC 
TCGACCGTGA CTGTGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298012.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153498.2 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa147547
Clone ID Related Accession (Same CDS sequence)	XM_015298012.2
Accession Version	XM_015298012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCG AGCTGCAGGC CGCGAGCGGC GGCCCCGGCG GCCTCGAGGC GGATTGCCAG 
GTTTTAAACA AGATGAAAGA GATAACAGGA ATTCAGGATG CAGATTTCCT TCACGCTGCT 
CTCAAGGCTG CTAAGGGAAA CCTGATGGAG GCACTCATCG TCCTCACAGA GGAGCGTGAT 
CAGGAGCCTG TTCAGAACAC AGCTGCTGCA GAGCCGTCCT CTTGGGAAGG CAGTGCAGTG 
GGCAAAGAGC CCCCACAAGG TGGTGCTGCG TTCGACCCTG AAAAGAAAGG AGATGTGCAC 
TCAGCTGTTG CCTACGGACA GCTGGAATCA CCAAAAGCTC ATGCTGCTGA AAGACCACAG 
GAGGTCCATT CTCCTGAACA CAAAAACCGC TCCAAAAGGA AACGCTGTGA AGTCTGGGGA 
GAGAATTCCA AACAGAGCGA CTGGAAGAGA GTGGGCGATT GGCCTGTGGG GATGAAGAAC 
ATCGGCAACA CGTGTTGGTT TAGTGCTGTC ATACAGTCTC TTTTCCAGTT GCCGCAGTTC 
CGGAGGTTGG TGCTCAGCTA CTCCTTCCCA CCAAACGTAC TTGAGAGCTG CCGGACGCGC 
ACTGGAAAAA GAAATATTGC ATTCATGCAA GAGCTTCAGT GTCTGTTTGC TTTAATGCTG 
GGAACACGTC GGAAGTTTGT GGACCCTTCT GCAGCACTGG AACTCTTAAG GGATGCGTTT 
AAATCAACCG AGGAGCAGCA GCAAGATGTG AGTGAGTTCA CACACAAACT GCTGGATTGG 
CTGGAAGATG CATTCCAGCT CGCTGTGAAT GTCAGAAGCC CTGGGGACAA ATCCGAAAAC 
CCAATGGTCC AACTTTTCTA TGGGACTTTC TTGACTGAGG GGGTCCACGA GGGCAATACT 
TTTTCCAAGA TCGAAACCTT TGGTCAGTAT CCCCTTCAGG TAAATGGTTA TCGAAACTTG 
AATGAGTGCT TGGAAGGAGC CATGGTGGAG GGAGAGATGG ATGAGGAGAC AGCAACTCAG 
TCAGTGAAAT ATGTACAGGA GCGCTGGTTC ACCAAACTCC CTCCTGTGCT GACCTTTGAA 
CTCTCCCGAT TTGAGTTCAA CCAATCCCTG GGACAGCCAG AGAAAATCCA CACAAAGCTG 
GAGTTCCCTC AGACTATTTA CATGGACAGG TACCTCTACT GCAGCAAAGA GCTTATTCAG 
ACAAAGAGAG AAGAAATGAA GAAATTGAAG GAGAAGATGC TGGTTCTGCA ACAGAAACTG 
GAAAGGTATA TGAAGTATGG CTCTGGCCCA GCACGCTTCC CACTGCCTGA CATGCTGCAA 
TATGTTCTGG AATTCATCAC TACAAAGCCT GCTGGAGCTG TGTCCTCTGC GTGCGTGTCT 
TCTACTGAGG ACTCTCAGAT GATGGACAGG CAGTCCCAGG GCGAGTCGCT CATTCTGGGC 
ACGCCTTCAC AGCCTGACAG CATGCTAGAT GGAAAGGATG GTAAGCCTGA AGATGAAGCT 
GTCCTATTGG CAAATTCCTC ACCTCAGCAA CAACTGAACG CGCCTCTGCA GCCTTCTGAA 
CCACCAGCAG AGATGTCAGA CTGTCCTGCT CCCCACGTGG TTTCTGAGGA AGAGATGAAC 
CTGGTTACGA CGTGTCTGCA GCGATGGAGG AATGAGATTG AGCAAGATGT GCGAGATCTG 
AAGGAGTCCA TCGCCAGAAT CAGCCTATCC ATTGACGAAA TGTACAGCGA CCCTCACCTC 
CAGCAGGTTC CTTATCACTT GCACGCTGTC TTGGTGCATG AAGGGCAAGC AAATGCTGGT 
CACTACTGGG CCTTCATATA TGACCAGCCT CGGAAAAGCT GGCTGAAATA CAATGACATC 
TCAGTGACAG AATCATCGTG GGAAGAGCTG GAGAGAGATT CATTTGGTGG CCTGAGGAAC 
GCCAGTGCTT ACTGCCTGAT GTACATAAGC GATAAGGTGT CCCACGTTGT TGCAGGTGAA 
GGTGACGGCT CAGAGGTTGG GCAGTTCCAG AAGGAGGTGG AGGCTTTGCC CCCAGAGCTG 
AGGCGTTACA TCCAGGAGGA CAACTGGCGG CTCGAGCAGG AGGCAGAAGA GTGGGAGGAG 
GAGCAGTCCT GCAAGATTCC TTCAACTGCT TCTGAATCTC AAGAGCTCTC TCCTGAGTCG 
GGGCTAGGTG ATGCCACATC TGATTTTGCA AGCTGTGTTG AAATGAATGA TCCACCTGCA 
GCCCACGAGC AGAGCCTGCG CTCTCTGTCC TCGGAGCACG CCATGATTGC AAAGGAGCAG 
ACTGCCAAAG CCATTGCCAA CGCAGCCAAC GTCTACGAGA AGAACGGCGT GGAGGCAGCG 
CTCTGCGAGC CAAAGGAGGT AGAACCAATG AAGGCCCAAC TGGGAGAAAC CACCTTTACA 
GTTCAGGCAG AGCAACCTCA GGATGCTAAA GAAGCAGAGC CCACTGCCCA GAGTAGCTCA 
CAGATCTCGG AAGTGGAAAT CCCCAGTGTG GGGAAGGTTC CTGTTAGATC TGATGCAGAT 
GGATATAATG AGGAGGTGAT GCTGAGTCCA GCCATGCAAG GTGTCATCCT GGCTATTGCA 
AAAGCCCGCC AGACCTTTGA TCGTGATGGG TCTGAAGCGG GGCTCGTTAA GGCATTCCAC 
GAGGAGTACT CCAGACTCTA TCTCCTTTCC AAAGAGACCC CGACCCCTCA GAACGATGCC 
CGCTTGCAAC ACGTGCTCGT TTACTTCCTT CAGAACGATG CTCCCCAGCA GATTGTGGAA 
CGGACCCTCC TCGAGCAGTT TGCAGACAAA AACCTCAGCT ACGATGAAAG GTCCATTAGC 
ATCATGAAGG TAGCACGAGA TAAACTGAAA GAGATCGGTC CTGATGAGGT CAATATGGAG 
GAGTATAAGA AGTGGCATGA GGACTACAGT CTTTTTCGGA AGGTGTCTGT TTACCTGCTT 
ACAGGGTTGG AGCTGTACCA GAATAGAAAA TACAAGGAGT CCCTGACCTA CCTGATCTAT 
GCCTACCAAA GCAACACCAC TCTGCTGAAG AAGGGAGCCA ACAGAGGAGT GAATGAATCG 
CTGATCACCT TGTATAGAAG AAAGTGTCTC TTGAAGCTGA ATGAGGTGGC ATCATCTCTG 
TTTGTAAGCT GTGAGGAAGC CCACGTGGCA GAGGGCATTA GCATCCTGAA TGAGCTGATC 
ATCCCCTGTA TGCACCTCAT TAACAACTTT GACATCTCCA GAGAAGACAT GGATGCCATT 
GAGGTCATGA GAAACCGCTG GTGCTCCTAT CTGGGACGAG AAGACATGGA CGCAAGCCTG 
CAGATCAGGC TGGGCGAGCT GCTCCCCCGC CTGCTGGACG GCTCCACGGA GGTGGTGGTG 
CTGAAGGAGC CCCCAAAGAT CCGTCCCAAC TCCCCATACG ACCTCTGCAG CCGCTTTGCA 
GCGGTGATGG AGTCCATCCA TGACGCCTCG ACCGTGACTG TGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153498.2
CDS	98..3565
Translation	MTAELQAASGGPGGLEADCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298012.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298012.2

ORF Insert Sequence:

ATGACGGCCG AGCTGCAGGC CGCGAGCGGC GGCCCCGGCG GCCTCGAGGC GGATTGCCAG 
GTTTTAAACA AGATGAAAGA GATAACAGGA ATTCAGGATG CAGATTTCCT TCACGCTGCT 
CTCAAGGCTG CTAAGGGAAA CCTGATGGAG GCACTCATCG TCCTCACAGA GGAGCGTGAT 
CAGGAGCCTG TTCAGAACAC AGCTGCTGCA GAGCCGTCCT CTTGGGAAGG CAGTGCAGTG 
GGCAAAGAGC CCCCACAAGG TGGTGCTGCG TTCGACCCTG AAAAGAAAGG AGATGTGCAC 
TCAGCTGTTG CCTACGGACA GCTGGAATCA CCAAAAGCTC ATGCTGCTGA AAGACCACAG 
GAGGTCCATT CTCCTGAACA CAAAAACCGC TCCAAAAGGA AACGCTGTGA AGTCTGGGGA 
GAGAATTCCA AACAGAGCGA CTGGAAGAGA GTGGGCGATT GGCCTGTGGG GATGAAGAAC 
ATCGGCAACA CGTGTTGGTT TAGTGCTGTC ATACAGTCTC TTTTCCAGTT GCCGCAGTTC 
CGGAGGTTGG TGCTCAGCTA CTCCTTCCCA CCAAACGTAC TTGAGAGCTG CCGGACGCGC 
ACTGGAAAAA GAAATATTGC ATTCATGCAA GAGCTTCAGT GTCTGTTTGC TTTAATGCTG 
GGAACACGTC GGAAGTTTGT GGACCCTTCT GCAGCACTGG AACTCTTAAG GGATGCGTTT 
AAATCAACCG AGGAGCAGCA GCAAGATGTG AGTGAGTTCA CACACAAACT GCTGGATTGG 
CTGGAAGATG CATTCCAGCT CGCTGTGAAT GTCAGAAGCC CTGGGGACAA ATCCGAAAAC 
CCAATGGTCC AACTTTTCTA TGGGACTTTC TTGACTGAGG GGGTCCACGA GGGCAATACT 
TTTTCCAAGA TCGAAACCTT TGGTCAGTAT CCCCTTCAGG TAAATGGTTA TCGAAACTTG 
AATGAGTGCT TGGAAGGAGC CATGGTGGAG GGAGAGATGG ATGAGGAGAC AGCAACTCAG 
TCAGTGAAAT ATGTACAGGA GCGCTGGTTC ACCAAACTCC CTCCTGTGCT GACCTTTGAA 
CTCTCCCGAT TTGAGTTCAA CCAATCCCTG GGACAGCCAG AGAAAATCCA CACAAAGCTG 
GAGTTCCCTC AGACTATTTA CATGGACAGG TACCTCTACT GCAGCAAAGA GCTTATTCAG 
ACAAAGAGAG AAGAAATGAA GAAATTGAAG GAGAAGATGC TGGTTCTGCA ACAGAAACTG 
GAAAGGTATA TGAAGTATGG CTCTGGCCCA GCACGCTTCC CACTGCCTGA CATGCTGCAA 
TATGTTCTGG AATTCATCAC TACAAAGCCT GCTGGAGCTG TGTCCTCTGC GTGCGTGTCT 
TCTACTGAGG ACTCTCAGAT GATGGACAGG CAGTCCCAGG GCGAGTCGCT CATTCTGGGC 
ACGCCTTCAC AGCCTGACAG CATGCTAGAT GGAAAGGATG GTAAGCCTGA AGATGAAGCT 
GTCCTATTGG CAAATTCCTC ACCTCAGCAA CAACTGAACG CGCCTCTGCA GCCTTCTGAA 
CCACCAGCAG AGATGTCAGA CTGTCCTGCT CCCCACGTGG TTTCTGAGGA AGAGATGAAC 
CTGGTTACGA CGTGTCTGCA GCGATGGAGG AATGAGATTG AGCAAGATGT GCGAGATCTG 
AAGGAGTCCA TCGCCAGAAT CAGCCTATCC ATTGACGAAA TGTACAGCGA CCCTCACCTC 
CAGCAGGTTC CTTATCACTT GCACGCTGTC TTGGTGCATG AAGGGCAAGC AAATGCTGGT 
CACTACTGGG CCTTCATATA TGACCAGCCT CGGAAAAGCT GGCTGAAATA CAATGACATC 
TCAGTGACAG AATCATCGTG GGAAGAGCTG GAGAGAGATT CATTTGGTGG CCTGAGGAAC 
GCCAGTGCTT ACTGCCTGAT GTACATAAGC GATAAGGTGT CCCACGTTGT TGCAGGTGAA 
GGTGACGGCT CAGAGGTTGG GCAGTTCCAG AAGGAGGTGG AGGCTTTGCC CCCAGAGCTG 
AGGCGTTACA TCCAGGAGGA CAACTGGCGG CTCGAGCAGG AGGCAGAAGA GTGGGAGGAG 
GAGCAGTCCT GCAAGATTCC TTCAACTGCT TCTGAATCTC AAGAGCTCTC TCCTGAGTCG 
GGGCTAGGTG ATGCCACATC TGATTTTGCA AGCTGTGTTG AAATGAATGA TCCACCTGCA 
GCCCACGAGC AGAGCCTGCG CTCTCTGTCC TCGGAGCACG CCATGATTGC AAAGGAGCAG 
ACTGCCAAAG CCATTGCCAA CGCAGCCAAC GTCTACGAGA AGAACGGCGT GGAGGCAGCG 
CTCTGCGAGC CAAAGGAGGT AGAACCAATG AAGGCCCAAC TGGGAGAAAC CACCTTTACA 
GTTCAGGCAG AGCAACCTCA GGATGCTAAA GAAGCAGAGC CCACTGCCCA GAGTAGCTCA 
CAGATCTCGG AAGTGGAAAT CCCCAGTGTG GGGAAGGTTC CTGTTAGATC TGATGCAGAT 
GGATATAATG AGGAGGTGAT GCTGAGTCCA GCCATGCAAG GTGTCATCCT GGCTATTGCA 
AAAGCCCGCC AGACCTTTGA TCGTGATGGG TCTGAAGCGG GGCTCGTTAA GGCATTCCAC 
GAGGAGTACT CCAGACTCTA TCTCCTTTCC AAAGAGACCC CGACCCCTCA GAACGATGCC 
CGCTTGCAAC ACGTGCTCGT TTACTTCCTT CAGAACGATG CTCCCCAGCA GATTGTGGAA 
CGGACCCTCC TCGAGCAGTT TGCAGACAAA AACCTCAGCT ACGATGAAAG GTCCATTAGC 
ATCATGAAGG TAGCACGAGA TAAACTGAAA GAGATCGGTC CTGATGAGGT CAATATGGAG 
GAGTATAAGA AGTGGCATGA GGACTACAGT CTTTTTCGGA AGGTGTCTGT TTACCTGCTT 
ACAGGGTTGG AGCTGTACCA GAATAGAAAA TACAAGGAGT CCCTGACCTA CCTGATCTAT 
GCCTACCAAA GCAACACCAC TCTGCTGAAG AAGGGAGCCA ACAGAGGAGT GAATGAATCG 
CTGATCACCT TGTATAGAAG AAAGTGTCTC TTGAAGCTGA ATGAGGTGGC ATCATCTCTG 
TTTGTAAGCT GTGAGGAAGC CCACGTGGCA GAGGGCATTA GCATCCTGAA TGAGCTGATC 
ATCCCCTGTA TGCACCTCAT TAACAACTTT GACATCTCCA GAGAAGACAT GGATGCCATT 
GAGGTCATGA GAAACCGCTG GTGCTCCTAT CTGGGACGAG AAGACATGGA CGCAAGCCTG 
CAGATCAGGC TGGGCGAGCT GCTCCCCCGC CTGCTGGACG GCTCCACGGA GGTGGTGGTG 
CTGAAGGAGC CCCCAAAGAT CCGTCCCAAC TCCCCATACG ACCTCTGCAG CCGCTTTGCA 
GCGGTGATGG AGTCCATCCA TGACGCCTCG ACCGTGACTG TGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298019.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153505.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X10, mRNA.

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CloneID	OGa45821
Clone ID Related Accession (Same CDS sequence)	XM_015298019.2 , XM_015298019.1 , XM_015298012.1
Accession Version	XM_015298019.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGTGATGC TGAGTCCAGC CATGCAAGGT GTCATCCTGG CTATTGCAAA AGCCCGCCAG 
ACCTTTGATC GTGATGGGTC TGAAGCGGGG CTCGTTAAGG CATTCCACGA GGAGTACTCC 
AGACTCTATC TCCTTTCCAA AGAGACCCCG ACCCCTCAGA ACGATGCCCG CTTGCAACAC 
GTGCTCGTTT ACTTCCTTCA GAACGATGCT CCCCAGCAGA TTGTGGAACG GACCCTCCTC 
GAGCAGTTTG CAGACAAAAA CCTCAGCTAC GATGAAAGGT CCATTAGCAT CATGAAGGTA 
GCACGAGATA AACTGAAAGA GATCGGTCCT GATGAGGTCA ATATGGAGGA GTATAAGAAG 
TGGCATGAGG ACTACAGTCT TTTTCGGAAG GTGTCTGTTT ACCTGCTTAC AGGGTTGGAG 
CTGTACCAGA ATAGAAAATA CAAGGAGTCC CTGACCTACC TGATCTATGC CTACCAAAGC 
AACACCACTC TGCTGAAGAA GGGAGCCAAC AGAGGAGTGA ATGAATCGCT GATCACCTTG 
TATAGAAGAA AGTGTCTCTT GAAGCTGAAT GAGGTGGCAT CATCTCTGTT TGTAAGCTGT 
GAGGAAGCCC ACGTGGCAGA GGGCATTAGC ATCCTGAATG AGCTGATCAT CCCCTGTATG 
CACCTCATTA ACAACTTTGA CATCTCCAGA GAAGACATGG ATGCCATTGA GGTCATGAGA 
AACCGCTGGT GCTCCTATCT GGGACGAGAA GACATGGACG CAAGCCTGCA GATCAGGCTG 
GGCGAGCTGC TCCCCCGCCT GCTGGACGGC TCCACGGAGG TGGTGGTGCT GAAGGAGCCC 
CCAAAGATCC GTCCCAACTC CCCATACGAC CTCTGCAGCC GCTTTGCAGC GGTGATGGAG 
TCCATCCATG ACGCCTCGAC CGTGACTGTG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153505.1
CDS	47..3442
Translation	MKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298019.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298019.2

ORF Insert Sequence:

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGTGATGC TGAGTCCAGC CATGCAAGGT GTCATCCTGG CTATTGCAAA AGCCCGCCAG 
ACCTTTGATC GTGATGGGTC TGAAGCGGGG CTCGTTAAGG CATTCCACGA GGAGTACTCC 
AGACTCTATC TCCTTTCCAA AGAGACCCCG ACCCCTCAGA ACGATGCCCG CTTGCAACAC 
GTGCTCGTTT ACTTCCTTCA GAACGATGCT CCCCAGCAGA TTGTGGAACG GACCCTCCTC 
GAGCAGTTTG CAGACAAAAA CCTCAGCTAC GATGAAAGGT CCATTAGCAT CATGAAGGTA 
GCACGAGATA AACTGAAAGA GATCGGTCCT GATGAGGTCA ATATGGAGGA GTATAAGAAG 
TGGCATGAGG ACTACAGTCT TTTTCGGAAG GTGTCTGTTT ACCTGCTTAC AGGGTTGGAG 
CTGTACCAGA ATAGAAAATA CAAGGAGTCC CTGACCTACC TGATCTATGC CTACCAAAGC 
AACACCACTC TGCTGAAGAA GGGAGCCAAC AGAGGAGTGA ATGAATCGCT GATCACCTTG 
TATAGAAGAA AGTGTCTCTT GAAGCTGAAT GAGGTGGCAT CATCTCTGTT TGTAAGCTGT 
GAGGAAGCCC ACGTGGCAGA GGGCATTAGC ATCCTGAATG AGCTGATCAT CCCCTGTATG 
CACCTCATTA ACAACTTTGA CATCTCCAGA GAAGACATGG ATGCCATTGA GGTCATGAGA 
AACCGCTGGT GCTCCTATCT GGGACGAGAA GACATGGACG CAAGCCTGCA GATCAGGCTG 
GGCGAGCTGC TCCCCCGCCT GCTGGACGGC TCCACGGAGG TGGTGGTGCT GAAGGAGCCC 
CCAAAGATCC GTCCCAACTC CCCATACGAC CTCTGCAGCC GCTTTGCAGC GGTGATGGAG 
TCCATCCATG ACGCCTCGAC CGTGACTGTG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298019.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153505.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

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CloneID	OGa45821
Clone ID Related Accession (Same CDS sequence)	XM_015298019.2 , XM_015298019.1 , XM_015298012.1
Accession Version	XM_015298019.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGTGATGC TGAGTCCAGC CATGCAAGGT GTCATCCTGG CTATTGCAAA AGCCCGCCAG 
ACCTTTGATC GTGATGGGTC TGAAGCGGGG CTCGTTAAGG CATTCCACGA GGAGTACTCC 
AGACTCTATC TCCTTTCCAA AGAGACCCCG ACCCCTCAGA ACGATGCCCG CTTGCAACAC 
GTGCTCGTTT ACTTCCTTCA GAACGATGCT CCCCAGCAGA TTGTGGAACG GACCCTCCTC 
GAGCAGTTTG CAGACAAAAA CCTCAGCTAC GATGAAAGGT CCATTAGCAT CATGAAGGTA 
GCACGAGATA AACTGAAAGA GATCGGTCCT GATGAGGTCA ATATGGAGGA GTATAAGAAG 
TGGCATGAGG ACTACAGTCT TTTTCGGAAG GTGTCTGTTT ACCTGCTTAC AGGGTTGGAG 
CTGTACCAGA ATAGAAAATA CAAGGAGTCC CTGACCTACC TGATCTATGC CTACCAAAGC 
AACACCACTC TGCTGAAGAA GGGAGCCAAC AGAGGAGTGA ATGAATCGCT GATCACCTTG 
TATAGAAGAA AGTGTCTCTT GAAGCTGAAT GAGGTGGCAT CATCTCTGTT TGTAAGCTGT 
GAGGAAGCCC ACGTGGCAGA GGGCATTAGC ATCCTGAATG AGCTGATCAT CCCCTGTATG 
CACCTCATTA ACAACTTTGA CATCTCCAGA GAAGACATGG ATGCCATTGA GGTCATGAGA 
AACCGCTGGT GCTCCTATCT GGGACGAGAA GACATGGACG CAAGCCTGCA GATCAGGCTG 
GGCGAGCTGC TCCCCCGCCT GCTGGACGGC TCCACGGAGG TGGTGGTGCT GAAGGAGCCC 
CCAAAGATCC GTCCCAACTC CCCATACGAC CTCTGCAGCC GCTTTGCAGC GGTGATGGAG 
TCCATCCATG ACGCCTCGAC CGTGACTGTG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153505.1
CDS	236..3631
Translation	MKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298019.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298019.1

ORF Insert Sequence:

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGTGATGC TGAGTCCAGC CATGCAAGGT GTCATCCTGG CTATTGCAAA AGCCCGCCAG 
ACCTTTGATC GTGATGGGTC TGAAGCGGGG CTCGTTAAGG CATTCCACGA GGAGTACTCC 
AGACTCTATC TCCTTTCCAA AGAGACCCCG ACCCCTCAGA ACGATGCCCG CTTGCAACAC 
GTGCTCGTTT ACTTCCTTCA GAACGATGCT CCCCAGCAGA TTGTGGAACG GACCCTCCTC 
GAGCAGTTTG CAGACAAAAA CCTCAGCTAC GATGAAAGGT CCATTAGCAT CATGAAGGTA 
GCACGAGATA AACTGAAAGA GATCGGTCCT GATGAGGTCA ATATGGAGGA GTATAAGAAG 
TGGCATGAGG ACTACAGTCT TTTTCGGAAG GTGTCTGTTT ACCTGCTTAC AGGGTTGGAG 
CTGTACCAGA ATAGAAAATA CAAGGAGTCC CTGACCTACC TGATCTATGC CTACCAAAGC 
AACACCACTC TGCTGAAGAA GGGAGCCAAC AGAGGAGTGA ATGAATCGCT GATCACCTTG 
TATAGAAGAA AGTGTCTCTT GAAGCTGAAT GAGGTGGCAT CATCTCTGTT TGTAAGCTGT 
GAGGAAGCCC ACGTGGCAGA GGGCATTAGC ATCCTGAATG AGCTGATCAT CCCCTGTATG 
CACCTCATTA ACAACTTTGA CATCTCCAGA GAAGACATGG ATGCCATTGA GGTCATGAGA 
AACCGCTGGT GCTCCTATCT GGGACGAGAA GACATGGACG CAAGCCTGCA GATCAGGCTG 
GGCGAGCTGC TCCCCCGCCT GCTGGACGGC TCCACGGAGG TGGTGGTGCT GAAGGAGCCC 
CCAAAGATCC GTCCCAACTC CCCATACGAC CTCTGCAGCC GCTTTGCAGC GGTGATGGAG 
TCCATCCATG ACGCCTCGAC CGTGACTGTG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298012.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153498.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

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CloneID	OGa45821
Clone ID Related Accession (Same CDS sequence)	XM_015298019.2 , XM_015298019.1 , XM_015298012.1
Accession Version	XM_015298012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGTGATGC TGAGTCCAGC CATGCAAGGT GTCATCCTGG CTATTGCAAA AGCCCGCCAG 
ACCTTTGATC GTGATGGGTC TGAAGCGGGG CTCGTTAAGG CATTCCACGA GGAGTACTCC 
AGACTCTATC TCCTTTCCAA AGAGACCCCG ACCCCTCAGA ACGATGCCCG CTTGCAACAC 
GTGCTCGTTT ACTTCCTTCA GAACGATGCT CCCCAGCAGA TTGTGGAACG GACCCTCCTC 
GAGCAGTTTG CAGACAAAAA CCTCAGCTAC GATGAAAGGT CCATTAGCAT CATGAAGGTA 
GCACGAGATA AACTGAAAGA GATCGGTCCT GATGAGGTCA ATATGGAGGA GTATAAGAAG 
TGGCATGAGG ACTACAGTCT TTTTCGGAAG GTGTCTGTTT ACCTGCTTAC AGGGTTGGAG 
CTGTACCAGA ATAGAAAATA CAAGGAGTCC CTGACCTACC TGATCTATGC CTACCAAAGC 
AACACCACTC TGCTGAAGAA GGGAGCCAAC AGAGGAGTGA ATGAATCGCT GATCACCTTG 
TATAGAAGAA AGTGTCTCTT GAAGCTGAAT GAGGTGGCAT CATCTCTGTT TGTAAGCTGT 
GAGGAAGCCC ACGTGGCAGA GGGCATTAGC ATCCTGAATG AGCTGATCAT CCCCTGTATG 
CACCTCATTA ACAACTTTGA CATCTCCAGA GAAGACATGG ATGCCATTGA GGTCATGAGA 
AACCGCTGGT GCTCCTATCT GGGACGAGAA GACATGGACG CAAGCCTGCA GATCAGGCTG 
GGCGAGCTGC TCCCCCGCCT GCTGGACGGC TCCACGGAGG TGGTGGTGCT GAAGGAGCCC 
CCAAAGATCC GTCCCAACTC CCCATACGAC CTCTGCAGCC GCTTTGCAGC GGTGATGGAG 
TCCATCCATG ACGCCTCGAC CGTGACTGTG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153498.1
CDS	126..3521
Translation	MKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298012.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298012.1

ORF Insert Sequence:

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGTGATGC TGAGTCCAGC CATGCAAGGT GTCATCCTGG CTATTGCAAA AGCCCGCCAG 
ACCTTTGATC GTGATGGGTC TGAAGCGGGG CTCGTTAAGG CATTCCACGA GGAGTACTCC 
AGACTCTATC TCCTTTCCAA AGAGACCCCG ACCCCTCAGA ACGATGCCCG CTTGCAACAC 
GTGCTCGTTT ACTTCCTTCA GAACGATGCT CCCCAGCAGA TTGTGGAACG GACCCTCCTC 
GAGCAGTTTG CAGACAAAAA CCTCAGCTAC GATGAAAGGT CCATTAGCAT CATGAAGGTA 
GCACGAGATA AACTGAAAGA GATCGGTCCT GATGAGGTCA ATATGGAGGA GTATAAGAAG 
TGGCATGAGG ACTACAGTCT TTTTCGGAAG GTGTCTGTTT ACCTGCTTAC AGGGTTGGAG 
CTGTACCAGA ATAGAAAATA CAAGGAGTCC CTGACCTACC TGATCTATGC CTACCAAAGC 
AACACCACTC TGCTGAAGAA GGGAGCCAAC AGAGGAGTGA ATGAATCGCT GATCACCTTG 
TATAGAAGAA AGTGTCTCTT GAAGCTGAAT GAGGTGGCAT CATCTCTGTT TGTAAGCTGT 
GAGGAAGCCC ACGTGGCAGA GGGCATTAGC ATCCTGAATG AGCTGATCAT CCCCTGTATG 
CACCTCATTA ACAACTTTGA CATCTCCAGA GAAGACATGG ATGCCATTGA GGTCATGAGA 
AACCGCTGGT GCTCCTATCT GGGACGAGAA GACATGGACG CAAGCCTGCA GATCAGGCTG 
GGCGAGCTGC TCCCCCGCCT GCTGGACGGC TCCACGGAGG TGGTGGTGCT GAAGGAGCCC 
CCAAAGATCC GTCCCAACTC CCCATACGAC CTCTGCAGCC GCTTTGCAGC GGTGATGGAG 
TCCATCCATG ACGCCTCGAC CGTGACTGTG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298013.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153499.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa45822
Clone ID Related Accession (Same CDS sequence)	XM_015298013.1
Accession Version	XM_015298013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGTGATGC TGAGTCCAGC CATGCAAGGT GTCATCCTGG CTATTGCAAA AGCCCGCCAG 
ACCTTTGATC GTGATGGGTC TGAAGCGGGG CTCGTTAAGG CATTCCACGA GGAGTACTCC 
AGACTCTATC TCCTTTCCAA AGAGACCCCG ACCCCTCAGA ACGATGCCCG CTTGCAACAC 
GTGCTCGTTT ACTTCCTTCA GAACGATGCT CCCCAGCAGA TTGTGGAACG GACCCTCCTC 
GAGCAGTTTG CAGACAAAAA CCTCAGCTAC GATGAAAGGT CCATTAGCAT CATGAAGGTA 
GCACGAGATA AACTGAAAGA GATCGGTCCT GATGAGGTCA ATATGGAGGA GTATAAGAAG 
TGGCATGAGG ACTACAGTCT TTTTCGGAAG GTGTCTGTTT ACCTGCTTAC AGGGTTGGAG 
CTGTACCAGA ATAGAAAATA CAAGGAGTCC CTGACCTACC TGATCTATGC CTACCAAAGC 
AACACCACTC TGCTGAAGAA GGGAGCCAAC AGAGGAGTGA ATGAATCGCT GATCACCTTG 
TATAGAAGAA AGTGTCTCTT GCTGAATGAG GTGGCATCAT CTCTGTTTGT AAGCTGTGAG 
GAAGCCCACG TGGCAGAGGG CATTAGCATC CTGAATGAGC TGATCATCCC CTGTATGCAC 
CTCATTAACA ACTTTGACAT CTCCAGAGAA GACATGGATG CCATTGAGGT CATGAGAAAC 
CGCTGGTGCT CCTATCTGGG ACGAGAAGAC ATGGACGCAA GCCTGCAGAT CAGGCTGGGC 
GAGCTGCTCC CCCGCCTGCT GGACGGCTCC ACGGAGGTGG TGGTGCTGAA GGAGCCCCCA 
AAGATCCGTC CCAACTCCCC ATACGACCTC TGCAGCCGCT TTGCAGCGGT GATGGAGTCC 
ATCCATGACG CCTCGACCGT GACTGTGAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153499.1
CDS	126..3518
Translation	MKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298013.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298013.1

ORF Insert Sequence:

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGTGATGC TGAGTCCAGC CATGCAAGGT GTCATCCTGG CTATTGCAAA AGCCCGCCAG 
ACCTTTGATC GTGATGGGTC TGAAGCGGGG CTCGTTAAGG CATTCCACGA GGAGTACTCC 
AGACTCTATC TCCTTTCCAA AGAGACCCCG ACCCCTCAGA ACGATGCCCG CTTGCAACAC 
GTGCTCGTTT ACTTCCTTCA GAACGATGCT CCCCAGCAGA TTGTGGAACG GACCCTCCTC 
GAGCAGTTTG CAGACAAAAA CCTCAGCTAC GATGAAAGGT CCATTAGCAT CATGAAGGTA 
GCACGAGATA AACTGAAAGA GATCGGTCCT GATGAGGTCA ATATGGAGGA GTATAAGAAG 
TGGCATGAGG ACTACAGTCT TTTTCGGAAG GTGTCTGTTT ACCTGCTTAC AGGGTTGGAG 
CTGTACCAGA ATAGAAAATA CAAGGAGTCC CTGACCTACC TGATCTATGC CTACCAAAGC 
AACACCACTC TGCTGAAGAA GGGAGCCAAC AGAGGAGTGA ATGAATCGCT GATCACCTTG 
TATAGAAGAA AGTGTCTCTT GCTGAATGAG GTGGCATCAT CTCTGTTTGT AAGCTGTGAG 
GAAGCCCACG TGGCAGAGGG CATTAGCATC CTGAATGAGC TGATCATCCC CTGTATGCAC 
CTCATTAACA ACTTTGACAT CTCCAGAGAA GACATGGATG CCATTGAGGT CATGAGAAAC 
CGCTGGTGCT CCTATCTGGG ACGAGAAGAC ATGGACGCAA GCCTGCAGAT CAGGCTGGGC 
GAGCTGCTCC CCCGCCTGCT GGACGGCTCC ACGGAGGTGG TGGTGCTGAA GGAGCCCCCA 
AAGATCCGTC CCAACTCCCC ATACGACCTC TGCAGCCGCT TTGCAGCGGT GATGGAGTCC 
ATCCATGACG CCTCGACCGT GACTGTGAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298014.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153500.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa45823
Clone ID Related Accession (Same CDS sequence)	XM_015298014.1
Accession Version	XM_015298014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGATCCA 
CCTGCAGCCC ACGAGCAGAG CCTGCGCTCT CTGTCCTCGG AGCACGCCAT GATTGCAAAG 
GAGCAGACTG CCAAAGCCAT TGCCAACGCA GCCAACGTCT ACGAGAAGAA CGGCGTGGAG 
GCAGCGCTCT GCGAGCCAAA GGAGGTAGAA CCAATGAAGG CCCAACTGGG AGAAACCACC 
TTTACAGTTC AGGCAGAGCA ACCTCAGGAT GCTAAAGAAG CAGAGCCCAC TGCCCAGAGT 
AGCTCACAGA TCTCGGAAGT GGAAATCCCC AGTGTGGGGA AGGTTCCTGT TAGATCTGAT 
GCAGATGGAT ATAATGAGGA GGTGATGCTG AGTCCAGCCA TGCAAGGTGT CATCCTGGCT 
ATTGCAAAAG CCCGCCAGAC CTTTGATCGT GATGGGTCTG AAGCGGGGCT CGTTAAGGCA 
TTCCACGAGG AGTACTCCAG ACTCTATCTC CTTTCCAAAG AGACCCCGAC CCCTCAGAAC 
GATGCCCGCT TGCAACACGT GCTCGTTTAC TTCCTTCAGA ACGATGCTCC CCAGCAGATT 
GTGGAACGGA CCCTCCTCGA GCAGTTTGCA GACAAAAACC TCAGCTACGA TGAAAGGTCC 
ATTAGCATCA TGAAGGTAGC ACGAGATAAA CTGAAAGAGA TCGGTCCTGA TGAGGTCAAT 
ATGGAGGAGT ATAAGAAGTG GCATGAGGAC TACAGTCTTT TTCGGAAGGT GTCTGTTTAC 
CTGCTTACAG GGTTGGAGCT GTACCAGAAT AGAAAATACA AGGAGTCCCT GACCTACCTG 
ATCTATGCCT ACCAAAGCAA CACCACTCTG CTGAAGAAGG GAGCCAACAG AGGAGTGAAT 
GAATCGCTGA TCACCTTGTA TAGAAGAAAG TGTCTCTTGA AGCTGAATGA GGTGGCATCA 
TCTCTGTTTG TAAGCTGTGA GGAAGCCCAC GTGGCAGAGG GCATTAGCAT CCTGAATGAG 
CTGATCATCC CCTGTATGCA CCTCATTAAC AACTTTGACA TCTCCAGAGA AGACATGGAT 
GCCATTGAGG TCATGAGAAA CCGCTGGTGC TCCTATCTGG GACGAGAAGA CATGGACGCA 
AGCCTGCAGA TCAGGCTGGG CGAGCTGCTC CCCCGCCTGC TGGACGGCTC CACGGAGGTG 
GTGGTGCTGA AGGAGCCCCC AAAGATCCGT CCCAACTCCC CATACGACCT CTGCAGCCGC 
TTTGCAGCGG TGATGGAGTC CATCCATGAC GCCTCGACCG TGACTGTGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153500.1
CDS	126..3479
Translation	MKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298014.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298014.1

ORF Insert Sequence:

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGATCCA 
CCTGCAGCCC ACGAGCAGAG CCTGCGCTCT CTGTCCTCGG AGCACGCCAT GATTGCAAAG 
GAGCAGACTG CCAAAGCCAT TGCCAACGCA GCCAACGTCT ACGAGAAGAA CGGCGTGGAG 
GCAGCGCTCT GCGAGCCAAA GGAGGTAGAA CCAATGAAGG CCCAACTGGG AGAAACCACC 
TTTACAGTTC AGGCAGAGCA ACCTCAGGAT GCTAAAGAAG CAGAGCCCAC TGCCCAGAGT 
AGCTCACAGA TCTCGGAAGT GGAAATCCCC AGTGTGGGGA AGGTTCCTGT TAGATCTGAT 
GCAGATGGAT ATAATGAGGA GGTGATGCTG AGTCCAGCCA TGCAAGGTGT CATCCTGGCT 
ATTGCAAAAG CCCGCCAGAC CTTTGATCGT GATGGGTCTG AAGCGGGGCT CGTTAAGGCA 
TTCCACGAGG AGTACTCCAG ACTCTATCTC CTTTCCAAAG AGACCCCGAC CCCTCAGAAC 
GATGCCCGCT TGCAACACGT GCTCGTTTAC TTCCTTCAGA ACGATGCTCC CCAGCAGATT 
GTGGAACGGA CCCTCCTCGA GCAGTTTGCA GACAAAAACC TCAGCTACGA TGAAAGGTCC 
ATTAGCATCA TGAAGGTAGC ACGAGATAAA CTGAAAGAGA TCGGTCCTGA TGAGGTCAAT 
ATGGAGGAGT ATAAGAAGTG GCATGAGGAC TACAGTCTTT TTCGGAAGGT GTCTGTTTAC 
CTGCTTACAG GGTTGGAGCT GTACCAGAAT AGAAAATACA AGGAGTCCCT GACCTACCTG 
ATCTATGCCT ACCAAAGCAA CACCACTCTG CTGAAGAAGG GAGCCAACAG AGGAGTGAAT 
GAATCGCTGA TCACCTTGTA TAGAAGAAAG TGTCTCTTGA AGCTGAATGA GGTGGCATCA 
TCTCTGTTTG TAAGCTGTGA GGAAGCCCAC GTGGCAGAGG GCATTAGCAT CCTGAATGAG 
CTGATCATCC CCTGTATGCA CCTCATTAAC AACTTTGACA TCTCCAGAGA AGACATGGAT 
GCCATTGAGG TCATGAGAAA CCGCTGGTGC TCCTATCTGG GACGAGAAGA CATGGACGCA 
AGCCTGCAGA TCAGGCTGGG CGAGCTGCTC CCCCGCCTGC TGGACGGCTC CACGGAGGTG 
GTGGTGCTGA AGGAGCCCCC AAAGATCCGT CCCAACTCCC CATACGACCT CTGCAGCCGC 
TTTGCAGCGG TGATGGAGTC CATCCATGAC GCCTCGACCG TGACTGTGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298015.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153501.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa45824
Clone ID Related Accession (Same CDS sequence)	XM_015298015.1
Accession Version	XM_015298015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGCATTCC ACGAGGAGTA CTCCAGACTC TATCTCCTTT CCAAAGAGAC CCCGACCCCT 
CAGAACGATG CCCGCTTGCA ACACGTGCTC GTTTACTTCC TTCAGAACGA TGCTCCCCAG 
CAGATTGTGG AACGGACCCT CCTCGAGCAG TTTGCAGACA AAAACCTCAG CTACGATGAA 
AGGTCCATTA GCATCATGAA GGTAGCACGA GATAAACTGA AAGAGATCGG TCCTGATGAG 
GTCAATATGG AGGAGTATAA GAAGTGGCAT GAGGACTACA GTCTTTTTCG GAAGGTGTCT 
GTTTACCTGC TTACAGGGTT GGAGCTGTAC CAGAATAGAA AATACAAGGA GTCCCTGACC 
TACCTGATCT ATGCCTACCA AAGCAACACC ACTCTGCTGA AGAAGGGAGC CAACAGAGGA 
GTGAATGAAT CGCTGATCAC CTTGTATAGA AGAAAGTGTC TCTTGAAGCT GAATGAGGTG 
GCATCATCTC TGTTTGTAAG CTGTGAGGAA GCCCACGTGG CAGAGGGCAT TAGCATCCTG 
AATGAGCTGA TCATCCCCTG TATGCACCTC ATTAACAACT TTGACATCTC CAGAGAAGAC 
ATGGATGCCA TTGAGGTCAT GAGAAACCGC TGGTGCTCCT ATCTGGGACG AGAAGACATG 
GACGCAAGCC TGCAGATCAG GCTGGGCGAG CTGCTCCCCC GCCTGCTGGA CGGCTCCACG 
GAGGTGGTGG TGCTGAAGGA GCCCCCAAAG ATCCGTCCCA ACTCCCCATA CGACCTCTGC 
AGCCGCTTTG CAGCGGTGAT GGAGTCCATC CATGACGCCT CGACCGTGAC TGTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153501.1
CDS	126..3425
Translation	MKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEPKEVEPMKAQLGETTFTVQAEQPQDAKEAEPTAQSSSQISEVEIPSVGKVPVRSDADGYNEEAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298015.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298015.1

ORF Insert Sequence:

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGCCA 
AAGGAGGTAG AACCAATGAA GGCCCAACTG GGAGAAACCA CCTTTACAGT TCAGGCAGAG 
CAACCTCAGG ATGCTAAAGA AGCAGAGCCC ACTGCCCAGA GTAGCTCACA GATCTCGGAA 
GTGGAAATCC CCAGTGTGGG GAAGGTTCCT GTTAGATCTG ATGCAGATGG ATATAATGAG 
GAGGCATTCC ACGAGGAGTA CTCCAGACTC TATCTCCTTT CCAAAGAGAC CCCGACCCCT 
CAGAACGATG CCCGCTTGCA ACACGTGCTC GTTTACTTCC TTCAGAACGA TGCTCCCCAG 
CAGATTGTGG AACGGACCCT CCTCGAGCAG TTTGCAGACA AAAACCTCAG CTACGATGAA 
AGGTCCATTA GCATCATGAA GGTAGCACGA GATAAACTGA AAGAGATCGG TCCTGATGAG 
GTCAATATGG AGGAGTATAA GAAGTGGCAT GAGGACTACA GTCTTTTTCG GAAGGTGTCT 
GTTTACCTGC TTACAGGGTT GGAGCTGTAC CAGAATAGAA AATACAAGGA GTCCCTGACC 
TACCTGATCT ATGCCTACCA AAGCAACACC ACTCTGCTGA AGAAGGGAGC CAACAGAGGA 
GTGAATGAAT CGCTGATCAC CTTGTATAGA AGAAAGTGTC TCTTGAAGCT GAATGAGGTG 
GCATCATCTC TGTTTGTAAG CTGTGAGGAA GCCCACGTGG CAGAGGGCAT TAGCATCCTG 
AATGAGCTGA TCATCCCCTG TATGCACCTC ATTAACAACT TTGACATCTC CAGAGAAGAC 
ATGGATGCCA TTGAGGTCAT GAGAAACCGC TGGTGCTCCT ATCTGGGACG AGAAGACATG 
GACGCAAGCC TGCAGATCAG GCTGGGCGAG CTGCTCCCCC GCCTGCTGGA CGGCTCCACG 
GAGGTGGTGG TGCTGAAGGA GCCCCCAAAG ATCCGTCCCA ACTCCCCATA CGACCTCTGC 
AGCCGCTTTG CAGCGGTGAT GGAGTCCATC CATGACGCCT CGACCGTGAC TGTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298016.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153502.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa45825
Clone ID Related Accession (Same CDS sequence)	XM_015298016.1
Accession Version	XM_015298016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGGTG 
ATGCTGAGTC CAGCCATGCA AGGTGTCATC CTGGCTATTG CAAAAGCCCG CCAGACCTTT 
GATCGTGATG GGTCTGAAGC GGGGCTCGTT AAGGCATTCC ACGAGGAGTA CTCCAGACTC 
TATCTCCTTT CCAAAGAGAC CCCGACCCCT CAGAACGATG CCCGCTTGCA ACACGTGCTC 
GTTTACTTCC TTCAGAACGA TGCTCCCCAG CAGATTGTGG AACGGACCCT CCTCGAGCAG 
TTTGCAGACA AAAACCTCAG CTACGATGAA AGGTCCATTA GCATCATGAA GGTAGCACGA 
GATAAACTGA AAGAGATCGG TCCTGATGAG GTCAATATGG AGGAGTATAA GAAGTGGCAT 
GAGGACTACA GTCTTTTTCG GAAGGTGTCT GTTTACCTGC TTACAGGGTT GGAGCTGTAC 
CAGAATAGAA AATACAAGGA GTCCCTGACC TACCTGATCT ATGCCTACCA AAGCAACACC 
ACTCTGCTGA AGAAGGGAGC CAACAGAGGA GTGAATGAAT CGCTGATCAC CTTGTATAGA 
AGAAAGTGTC TCTTGAAGCT GAATGAGGTG GCATCATCTC TGTTTGTAAG CTGTGAGGAA 
GCCCACGTGG CAGAGGGCAT TAGCATCCTG AATGAGCTGA TCATCCCCTG TATGCACCTC 
ATTAACAACT TTGACATCTC CAGAGAAGAC ATGGATGCCA TTGAGGTCAT GAGAAACCGC 
TGGTGCTCCT ATCTGGGACG AGAAGACATG GACGCAAGCC TGCAGATCAG GCTGGGCGAG 
CTGCTCCCCC GCCTGCTGGA CGGCTCCACG GAGGTGGTGG TGCTGAAGGA GCCCCCAAAG 
ATCCGTCCCA ACTCCCCATA CGACCTCTGC AGCCGCTTTG CAGCGGTGAT GGAGTCCATC 
CATGACGCCT CGACCGTGAC TGTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153502.1
CDS	126..3335
Translation	MKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLGDATSDFASCVEMNDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298016.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298016.1

ORF Insert Sequence:

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGGTGAT 
GCCACATCTG ATTTTGCAAG CTGTGTTGAA ATGAATGATC CACCTGCAGC CCACGAGCAG 
AGCCTGCGCT CTCTGTCCTC GGAGCACGCC ATGATTGCAA AGGAGCAGAC TGCCAAAGCC 
ATTGCCAACG CAGCCAACGT CTACGAGAAG AACGGCGTGG AGGCAGCGCT CTGCGAGGTG 
ATGCTGAGTC CAGCCATGCA AGGTGTCATC CTGGCTATTG CAAAAGCCCG CCAGACCTTT 
GATCGTGATG GGTCTGAAGC GGGGCTCGTT AAGGCATTCC ACGAGGAGTA CTCCAGACTC 
TATCTCCTTT CCAAAGAGAC CCCGACCCCT CAGAACGATG CCCGCTTGCA ACACGTGCTC 
GTTTACTTCC TTCAGAACGA TGCTCCCCAG CAGATTGTGG AACGGACCCT CCTCGAGCAG 
TTTGCAGACA AAAACCTCAG CTACGATGAA AGGTCCATTA GCATCATGAA GGTAGCACGA 
GATAAACTGA AAGAGATCGG TCCTGATGAG GTCAATATGG AGGAGTATAA GAAGTGGCAT 
GAGGACTACA GTCTTTTTCG GAAGGTGTCT GTTTACCTGC TTACAGGGTT GGAGCTGTAC 
CAGAATAGAA AATACAAGGA GTCCCTGACC TACCTGATCT ATGCCTACCA AAGCAACACC 
ACTCTGCTGA AGAAGGGAGC CAACAGAGGA GTGAATGAAT CGCTGATCAC CTTGTATAGA 
AGAAAGTGTC TCTTGAAGCT GAATGAGGTG GCATCATCTC TGTTTGTAAG CTGTGAGGAA 
GCCCACGTGG CAGAGGGCAT TAGCATCCTG AATGAGCTGA TCATCCCCTG TATGCACCTC 
ATTAACAACT TTGACATCTC CAGAGAAGAC ATGGATGCCA TTGAGGTCAT GAGAAACCGC 
TGGTGCTCCT ATCTGGGACG AGAAGACATG GACGCAAGCC TGCAGATCAG GCTGGGCGAG 
CTGCTCCCCC GCCTGCTGGA CGGCTCCACG GAGGTGGTGG TGCTGAAGGA GCCCCCAAAG 
ATCCGTCCCA ACTCCCCATA CGACCTCTGC AGCCGCTTTG CAGCGGTGAT GGAGTCCATC 
CATGACGCCT CGACCGTGAC TGTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( XM_015298017.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015153503.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

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CloneID	OGa45826
Clone ID Related Accession (Same CDS sequence)	XM_015298017.1
Accession Version	XM_015298017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGATCCA 
CCTGCAGCCC ACGAGCAGAG CCTGCGCTCT CTGTCCTCGG AGCACGCCAT GATTGCAAAG 
GAGCAGACTG CCAAAGCCAT TGCCAACGCA GCCAACGTCT ACGAGAAGAA CGGCGTGGAG 
GCAGCGCTCT GCGAGGTGAT GCTGAGTCCA GCCATGCAAG GTGTCATCCT GGCTATTGCA 
AAAGCCCGCC AGACCTTTGA TCGTGATGGG TCTGAAGCGG GGCTCGTTAA GGCATTCCAC 
GAGGAGTACT CCAGACTCTA TCTCCTTTCC AAAGAGACCC CGACCCCTCA GAACGATGCC 
CGCTTGCAAC ACGTGCTCGT TTACTTCCTT CAGAACGATG CTCCCCAGCA GATTGTGGAA 
CGGACCCTCC TCGAGCAGTT TGCAGACAAA AACCTCAGCT ACGATGAAAG GTCCATTAGC 
ATCATGAAGG TAGCACGAGA TAAACTGAAA GAGATCGGTC CTGATGAGGT CAATATGGAG 
GAGTATAAGA AGTGGCATGA GGACTACAGT CTTTTTCGGA AGGTGTCTGT TTACCTGCTT 
ACAGGGTTGG AGCTGTACCA GAATAGAAAA TACAAGGAGT CCCTGACCTA CCTGATCTAT 
GCCTACCAAA GCAACACCAC TCTGCTGAAG AAGGGAGCCA ACAGAGGAGT GAATGAATCG 
CTGATCACCT TGTATAGAAG AAAGTGTCTC TTGAAGCTGA ATGAGGTGGC ATCATCTCTG 
TTTGTAAGCT GTGAGGAAGC CCACGTGGCA GAGGGCATTA GCATCCTGAA TGAGCTGATC 
ATCCCCTGTA TGCACCTCAT TAACAACTTT GACATCTCCA GAGAAGACAT GGATGCCATT 
GAGGTCATGA GAAACCGCTG GTGCTCCTAT CTGGGACGAG AAGACATGGA CGCAAGCCTG 
CAGATCAGGC TGGGCGAGCT GCTCCCCCGC CTGCTGGACG GCTCCACGGA GGTGGTGGTG 
CTGAAGGAGC CCCCAAAGAT CCGTCCCAAC TCCCCATACG ACCTCTGCAG CCGCTTTGCA 
GCGGTGATGG AGTCCATCCA TGACGCCTCG ACCGTGACTG TGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015153503.1
CDS	126..3293
Translation	MKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIYMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARISLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEVMLSPAMQGVILAIAKARQTFDRDGSEAGLVKAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	XM_015298017.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298017.1

ORF Insert Sequence:

ATGAAAGAGA TAACAGGAAT TCAGGATGCA GATTTCCTTC ACGCTGCTCT CAAGGCTGCT 
AAGGGAAACC TGATGGAGGC ACTCATCGTC CTCACAGAGG AGCGTGATCA GGAGCCTGTT 
CAGAACACAG CTGCTGCAGA GCCGTCCTCT TGGGAAGGCA GTGCAGTGGG CAAAGAGCCC 
CCACAAGGTG GTGCTGCGTT CGACCCTGAA AAGAAAGGAG ATGTGCACTC AGCTGTTGCC 
TACGGACAGC TGGAATCACC AAAAGCTCAT GCTGCTGAAA GACCACAGGA GGTCCATTCT 
CCTGAACACA AAAACCGCTC CAAAAGGAAA CGCTGTGAAG TCTGGGGAGA GAATTCCAAA 
CAGAGCGACT GGAAGAGAGT GGGCGATTGG CCTGTGGGGA TGAAGAACAT CGGCAACACG 
TGTTGGTTTA GTGCTGTCAT ACAGTCTCTT TTCCAGTTGC CGCAGTTCCG GAGGTTGGTG 
CTCAGCTACT CCTTCCCACC AAACGTACTT GAGAGCTGCC GGACGCGCAC TGGAAAAAGA 
AATATTGCAT TCATGCAAGA GCTTCAGTGT CTGTTTGCTT TAATGCTGGG AACACGTCGG 
AAGTTTGTGG ACCCTTCTGC AGCACTGGAA CTCTTAAGGG ATGCGTTTAA ATCAACCGAG 
GAGCAGCAGC AAGATGTGAG TGAGTTCACA CACAAACTGC TGGATTGGCT GGAAGATGCA 
TTCCAGCTCG CTGTGAATGT CAGAAGCCCT GGGGACAAAT CCGAAAACCC AATGGTCCAA 
CTTTTCTATG GGACTTTCTT GACTGAGGGG GTCCACGAGG GCAATACTTT TTCCAAGATC 
GAAACCTTTG GTCAGTATCC CCTTCAGGTA AATGGTTATC GAAACTTGAA TGAGTGCTTG 
GAAGGAGCCA TGGTGGAGGG AGAGATGGAT GAGGAGACAG CAACTCAGTC AGTGAAATAT 
GTACAGGAGC GCTGGTTCAC CAAACTCCCT CCTGTGCTGA CCTTTGAACT CTCCCGATTT 
GAGTTCAACC AATCCCTGGG ACAGCCAGAG AAAATCCACA CAAAGCTGGA GTTCCCTCAG 
ACTATTTACA TGGACAGGTA CCTCTACTGC AGCAAAGAGC TTATTCAGAC AAAGAGAGAA 
GAAATGAAGA AATTGAAGGA GAAGATGCTG GTTCTGCAAC AGAAACTGGA AAGGTATATG 
AAGTATGGCT CTGGCCCAGC ACGCTTCCCA CTGCCTGACA TGCTGCAATA TGTTCTGGAA 
TTCATCACTA CAAAGCCTGC TGGAGCTGTG TCCTCTGCGT GCGTGTCTTC TACTGAGGAC 
TCTCAGATGA TGGACAGGCA GTCCCAGGGC GAGTCGCTCA TTCTGGGCAC GCCTTCACAG 
CCTGACAGCA TGCTAGATGG AAAGGATGGT AAGCCTGAAG ATGAAGCTGT CCTATTGGCA 
AATTCCTCAC CTCAGCAACA ACTGAACGCG CCTCTGCAGC CTTCTGAACC ACCAGCAGAG 
ATGTCAGACT GTCCTGCTCC CCACGTGGTT TCTGAGGAAG AGATGAACCT GGTTACGACG 
TGTCTGCAGC GATGGAGGAA TGAGATTGAG CAAGATGTGC GAGATCTGAA GGAGTCCATC 
GCCAGAATCA GCCTATCCAT TGACGAAATG TACAGCGACC CTCACCTCCA GCAGGTTCCT 
TATCACTTGC ACGCTGTCTT GGTGCATGAA GGGCAAGCAA ATGCTGGTCA CTACTGGGCC 
TTCATATATG ACCAGCCTCG GAAAAGCTGG CTGAAATACA ATGACATCTC AGTGACAGAA 
TCATCGTGGG AAGAGCTGGA GAGAGATTCA TTTGGTGGCC TGAGGAACGC CAGTGCTTAC 
TGCCTGATGT ACATAAGCGA TAAGGTGTCC CACGTTGTTG CAGGTGAAGG TGACGGCTCA 
GAGGTTGGGC AGTTCCAGAA GGAGGTGGAG GCTTTGCCCC CAGAGCTGAG GCGTTACATC 
CAGGAGGACA ACTGGCGGCT CGAGCAGGAG GCAGAAGAGT GGGAGGAGGA GCAGTCCTGC 
AAGATTCCTT CAACTGCTTC TGAATCTCAA GAGCTCTCTC CTGAGTCGGG GCTAGATCCA 
CCTGCAGCCC ACGAGCAGAG CCTGCGCTCT CTGTCCTCGG AGCACGCCAT GATTGCAAAG 
GAGCAGACTG CCAAAGCCAT TGCCAACGCA GCCAACGTCT ACGAGAAGAA CGGCGTGGAG 
GCAGCGCTCT GCGAGGTGAT GCTGAGTCCA GCCATGCAAG GTGTCATCCT GGCTATTGCA 
AAAGCCCGCC AGACCTTTGA TCGTGATGGG TCTGAAGCGG GGCTCGTTAA GGCATTCCAC 
GAGGAGTACT CCAGACTCTA TCTCCTTTCC AAAGAGACCC CGACCCCTCA GAACGATGCC 
CGCTTGCAAC ACGTGCTCGT TTACTTCCTT CAGAACGATG CTCCCCAGCA GATTGTGGAA 
CGGACCCTCC TCGAGCAGTT TGCAGACAAA AACCTCAGCT ACGATGAAAG GTCCATTAGC 
ATCATGAAGG TAGCACGAGA TAAACTGAAA GAGATCGGTC CTGATGAGGT CAATATGGAG 
GAGTATAAGA AGTGGCATGA GGACTACAGT CTTTTTCGGA AGGTGTCTGT TTACCTGCTT 
ACAGGGTTGG AGCTGTACCA GAATAGAAAA TACAAGGAGT CCCTGACCTA CCTGATCTAT 
GCCTACCAAA GCAACACCAC TCTGCTGAAG AAGGGAGCCA ACAGAGGAGT GAATGAATCG 
CTGATCACCT TGTATAGAAG AAAGTGTCTC TTGAAGCTGA ATGAGGTGGC ATCATCTCTG 
TTTGTAAGCT GTGAGGAAGC CCACGTGGCA GAGGGCATTA GCATCCTGAA TGAGCTGATC 
ATCCCCTGTA TGCACCTCAT TAACAACTTT GACATCTCCA GAGAAGACAT GGATGCCATT 
GAGGTCATGA GAAACCGCTG GTGCTCCTAT CTGGGACGAG AAGACATGGA CGCAAGCCTG 
CAGATCAGGC TGGGCGAGCT GCTCCCCCGC CTGCTGGACG GCTCCACGGA GGTGGTGGTG 
CTGAAGGAGC CCCCAAAGAT CCGTCCCAAC TCCCCATACG ACCTCTGCAG CCGCTTTGCA 
GCGGTGATGG AGTCCATCCA TGACGCCTCG ACCGTGACTG TGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP28 ( NM_001031496.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001026667.1 Gallus gallus ubiquitin specific peptidase 28 (USP28), mRNA.

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CloneID	OGa27290
Clone ID Related Accession (Same CDS sequence)	NM_001031496.1
Accession Version	NM_001031496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Gallus gallus(chicken)
Product	ubiquitin carboxyl-terminal hydrolase 28
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ720849.1. On Aug 8, 2005 this sequence version replaced XM_425805.1. ##Evidence-Data-START## Transcript exon combination :: AJ720849.1 [ECO:0000332] ##Evidence-Data-END##

ATGACGGCCG AGCTGCAGGC CGCGAGCGGC GGCCCCGGCG GCCTCGAGGC GGATTGCCAG 
GTTTTAAACA AGATGAAAGA GATAACAGGA ATTCAGGATG CAGACTTCCT TCACGCTGCT 
CTCAAGGCTG CTAAGGGAAA CCTGATGGAG GCACTCATCG TCCTCACAGA GGAGCGTGAT 
CAGGAGCCTG TTCAGAACAC AGCTGCTGCA GAGCCGTCCT CTTGGGAAGG CAGTGCAGTG 
GGCAAAGAGC CCCCACAAGG TGGTGCTGCG TTCGACCCTG AAAAGAAAGG AGATGTGCAC 
TCAGCTGTTG CCTACGGACA GCTGGAATCA CCAAAAGCTC ATGCTGCTGA AAGACCACAG 
GAGGTCCATT CTCCTGAACA CAAAAACCGC TCCAAAAGGA AACGCTGTGA AGTCTGGGGA 
GAGAATTCCA AACAGAGCGA CTGGAAGAGA GTGGGCGATT GGCCTGTGGG GATGAAGAAC 
ATCGGCAACA CGTGTTGGTT TAGTGCTGTC ATACAGTCTC TTTTCCAGTT GCCGCAGTTC 
CGGAGGTTGG TGCTCAGCTA CTCCTTCCCA CCAAACGTAC TTGAGAGCTG CCGGACGCGC 
ACTGGAAAAA GAAATATTGC ATTCATGCAA GAGCTTCAGT GTCTGTTTGC TTTAATGCTG 
GGAACACGTC GGAAGTTTGT GGACCCTTCT GCAGCACTGG AACTCTTAAG GGATGCGTTT 
AAATCAACCG AGGAGCAGCA GCAAGATGTG AGTGAGTTCA CACACAAACT GCTGGATTGG 
CTGGAAGATG CATTCCAGCT CGCTGTGAAT GTCAGAAGCC CTGGGGACAA ATCCGAAAAC 
CCAATGGTCC AACTTTTCTA TGGGACTTTC TTGACTGAGG GGGTCCACGA GGGCAATACT 
TTTTCCAAGA TCGAAACCTT TGGTCAGTAT CCCCTTCAGG TAAATGGTTA TCGAAACTTG 
AATGAGTGCT TGGAAGGAGC CATGGTGGAG GGAGAGATGG ATGAGGAGAC AGCAACTCAG 
TCAGTGAAAT ATGTACAGGA GCGCTGGTTC ACCAAACTCC CTCCTGTGCT GACCTTTGAA 
CTCTCCCGAT TTGAGTTCAA CCAATCCCTG GGACAGCCAG AGAAAATCCA CACAAAGCTG 
GAGTTCCCTC AGACTATTTT CATGGACAGG TACCTCTACT GCAGCAAAGA GCTTATTCAG 
ACAAAGAGAG AAGAAATGAA GAAATTGAAG GAGAAGATGC TGGTTCTGCA ACAGAAACTG 
GAAAGGTATA TGAAGTACGG CTCTGGCCCA GCACGCTTCC CACTGCCTGA CATGCTGCAA 
TATGTTCTGG AATTCATCAC TACAAAGCCT GCTGGAGCTG TGTCCTCTGC GTGCGTGTCT 
TCTACTGAGG ACTCTCAGAT GATGGACAGG CAGTCCCAGG GCGAGTCGCT CATTCTGGGC 
ACGCCTTCAC AGCCTGACAG CATGCTAGAT GGAAAGGATG GTAAGCCTGA AGATGAAGCT 
GTCCTATTGG CAAATTCCTC ACCTCAGCAA CAACTGAACG CGCCTCTGCA GCCTTCTGAA 
CCACCAGCAG AGATGTCAGA CTGTCCTGCT CCCCACGTGG TTTCTGAGGA AGAGATGAAC 
CTGGTTACGA CGTGTCTGCA GCGATGGAGG AATGAGATTG AGCAAGATGT GCGAGATCTG 
AAGGAGTCCA TTGCCAGAGT CAGCCTATCC ATTGACGAAA TGTACAGCGA CCCTCACCTC 
CAGCAGGTTC CTTATCACTT GCACGCTGTC TTGGTGCATG AAGGGCAAGC AAATGCTGGT 
CACTACTGGG CCTTCATATA TGACCAGCCT CGGAAAAGCT GGCTGAAATA CAATGACATC 
TCAGTGACAG AATCATCGTG GGAAGAGCTG GAGAGAGATT CATTTGGTGG CCTGAGGAAC 
GCCAGTGCTT ACTGCCTGAT GTACATAAGC GATAAGGTGT CCCACGTTGT TGCAGGTGAA 
GGTGACGGCT CAGAGGTCGG GCAGTTCCAG AAGGAGGTGG AGGCTTTGCC CCCAGAGCTG 
AGGCGTTACA TCCAGGAGGA CAACTGGCGG CTCGAGCAGG AGGCAGAAGA GTGGGAGGAG 
GAGCAGTCCT GCAAGATTCC TTCAACTGCT TCTGAATCTC AAGAGCTCTC TCCTGAGTCG 
GGGCTAGATC CACCTGCAGC CCACGAGCAG AGCCTGCGCT CTCTGTCCTC GGAGCACGCC 
ATGATTGCAA AGGAGCAGAC TGCTAAAGCC ATTGCCAACG CAGCCAACGT CTACGAGAAG 
AACGGCGTGG AGGCAGCGCT CTGCGAGGCA TTCCACGAGG AGTACTCCAG ACTCTATCTC 
CTTTCCAAAG AGACCCCGAC CCCTCAGAAC GATGCCCGCT TGCAACACGT GCTCGTTTAC 
TTCCTTCAGA ACGATGCTCC CCAGCAGATT GTGGAACGGA CCCTCCTCGA GCAGTTTGCA 
GACAAAAACC TCAGCTACGA TGAAAGGTCC ATTAGCATCA TGAAGGTAGC ACGAGATAAA 
CTGAAAGAGA TCGGTCCTGA TGAGGTCAAT ATGGAGGAGT ATAAGAAGTG GCATGAGGAC 
TACAGTCTTT TTCGGAAGGT GTCTGTTTAC CTGCTTACAG GGTTGGAGCT GTACCAGAAT 
AGAAAGTACA AGGAGTCCCT GACCTACCTG ATCTATGCCT ACCAAAGCAA CACCACTCTG 
CTGAAGAAGG GAGCCAACAG AGGAGTGAAT GAATCGCTGA TCACCTTGTA TAGAAGAAAG 
TGTCTCTTGA AGCTGAATGA GGTGGCATCA TCTCTGTTTG TAAGCTGTGA GGAAGCCCAT 
GTGGCAGAGG GCATTAGCAT CCTGAATGAG CTGATCATCC CCTGTATGCA CCTCATTAAC 
AACTTTGACA TCTCCAGAGA AGACATGGAT GCCATTGAGG TCATGAGAAA CCGCTGGTGC 
TCCTATCTGG GACGAGAAGA CATGGACGCA AGCCTGCAGA TCAGGCTGGG CGAGCTGCTC 
CCCCGCCTGC TGGACGGCTC CACGGAGGTG GTGGTGCTGA AGGAGCCCCC AAAGATCCGT 
CCCAACTCCC CATACGACCT CTGCAGCCGC TTTGCAGCGG TGATGGAGTC CATCCATGAC 
GCCTCGACTG TGACTGTGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026667.1
CDS	62..3205
Translation	MTAELQAASGGPGGLEADCQVLNKMKEITGIQDADFLHAALKAAKGNLMEALIVLTEERDQEPVQNTAAAEPSSWEGSAVGKEPPQGGAAFDPEKKGDVHSAVAYGQLESPKAHAAERPQEVHSPEHKNRSKRKRCEVWGENSKQSDWKRVGDWPVGMKNIGNTCWFSAVIQSLFQLPQFRRLVLSYSFPPNVLESCRTRTGKRNIAFMQELQCLFALMLGTRRKFVDPSAALELLRDAFKSTEEQQQDVSEFTHKLLDWLEDAFQLAVNVRSPGDKSENPMVQLFYGTFLTEGVHEGNTFSKIETFGQYPLQVNGYRNLNECLEGAMVEGEMDEETATQSVKYVQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHTKLEFPQTIFMDRYLYCSKELIQTKREEMKKLKEKMLVLQQKLERYMKYGSGPARFPLPDMLQYVLEFITTKPAGAVSSACVSSTEDSQMMDRQSQGESLILGTPSQPDSMLDGKDGKPEDEAVLLANSSPQQQLNAPLQPSEPPAEMSDCPAPHVVSEEEMNLVTTCLQRWRNEIEQDVRDLKESIARVSLSIDEMYSDPHLQQVPYHLHAVLVHEGQANAGHYWAFIYDQPRKSWLKYNDISVTESSWEELERDSFGGLRNASAYCLMYISDKVSHVVAGEGDGSEVGQFQKEVEALPPELRRYIQEDNWRLEQEAEEWEEEQSCKIPSTASESQELSPESGLDPPAAHEQSLRSLSSEHAMIAKEQTAKAIANAANVYEKNGVEAALCEAFHEEYSRLYLLSKETPTPQNDARLQHVLVYFLQNDAPQQIVERTLLEQFADKNLSYDERSISIMKVARDKLKEIGPDEVNMEEYKKWHEDYSLFRKVSVYLLTGLELYQNRKYKESLTYLIYAYQSNTTLLKKGANRGVNESLITLYRRKCLLKLNEVASSLFVSCEEAHVAEGISILNELIIPCMHLINNFDISREDMDAIEVMRNRWCSYLGREDMDASLQIRLGELLPRLLDGSTEVVVLKEPPKIRPNSPYDLCSRFAAVMESIHDASTVTVK

Target ORF information:

RefSeq Version	NM_001031496.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ubiquitin specific peptidase 28 (USP28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031496.1

ORF Insert Sequence:

ATGACGGCCG AGCTGCAGGC CGCGAGCGGC GGCCCCGGCG GCCTCGAGGC GGATTGCCAG 
GTTTTAAACA AGATGAAAGA GATAACAGGA ATTCAGGATG CAGACTTCCT TCACGCTGCT 
CTCAAGGCTG CTAAGGGAAA CCTGATGGAG GCACTCATCG TCCTCACAGA GGAGCGTGAT 
CAGGAGCCTG TTCAGAACAC AGCTGCTGCA GAGCCGTCCT CTTGGGAAGG CAGTGCAGTG 
GGCAAAGAGC CCCCACAAGG TGGTGCTGCG TTCGACCCTG AAAAGAAAGG AGATGTGCAC 
TCAGCTGTTG CCTACGGACA GCTGGAATCA CCAAAAGCTC ATGCTGCTGA AAGACCACAG 
GAGGTCCATT CTCCTGAACA CAAAAACCGC TCCAAAAGGA AACGCTGTGA AGTCTGGGGA 
GAGAATTCCA AACAGAGCGA CTGGAAGAGA GTGGGCGATT GGCCTGTGGG GATGAAGAAC 
ATCGGCAACA CGTGTTGGTT TAGTGCTGTC ATACAGTCTC TTTTCCAGTT GCCGCAGTTC 
CGGAGGTTGG TGCTCAGCTA CTCCTTCCCA CCAAACGTAC TTGAGAGCTG CCGGACGCGC 
ACTGGAAAAA GAAATATTGC ATTCATGCAA GAGCTTCAGT GTCTGTTTGC TTTAATGCTG 
GGAACACGTC GGAAGTTTGT GGACCCTTCT GCAGCACTGG AACTCTTAAG GGATGCGTTT 
AAATCAACCG AGGAGCAGCA GCAAGATGTG AGTGAGTTCA CACACAAACT GCTGGATTGG 
CTGGAAGATG CATTCCAGCT CGCTGTGAAT GTCAGAAGCC CTGGGGACAA ATCCGAAAAC 
CCAATGGTCC AACTTTTCTA TGGGACTTTC TTGACTGAGG GGGTCCACGA GGGCAATACT 
TTTTCCAAGA TCGAAACCTT TGGTCAGTAT CCCCTTCAGG TAAATGGTTA TCGAAACTTG 
AATGAGTGCT TGGAAGGAGC CATGGTGGAG GGAGAGATGG ATGAGGAGAC AGCAACTCAG 
TCAGTGAAAT ATGTACAGGA GCGCTGGTTC ACCAAACTCC CTCCTGTGCT GACCTTTGAA 
CTCTCCCGAT TTGAGTTCAA CCAATCCCTG GGACAGCCAG AGAAAATCCA CACAAAGCTG 
GAGTTCCCTC AGACTATTTT CATGGACAGG TACCTCTACT GCAGCAAAGA GCTTATTCAG 
ACAAAGAGAG AAGAAATGAA GAAATTGAAG GAGAAGATGC TGGTTCTGCA ACAGAAACTG 
GAAAGGTATA TGAAGTACGG CTCTGGCCCA GCACGCTTCC CACTGCCTGA CATGCTGCAA 
TATGTTCTGG AATTCATCAC TACAAAGCCT GCTGGAGCTG TGTCCTCTGC GTGCGTGTCT 
TCTACTGAGG ACTCTCAGAT GATGGACAGG CAGTCCCAGG GCGAGTCGCT CATTCTGGGC 
ACGCCTTCAC AGCCTGACAG CATGCTAGAT GGAAAGGATG GTAAGCCTGA AGATGAAGCT 
GTCCTATTGG CAAATTCCTC ACCTCAGCAA CAACTGAACG CGCCTCTGCA GCCTTCTGAA 
CCACCAGCAG AGATGTCAGA CTGTCCTGCT CCCCACGTGG TTTCTGAGGA AGAGATGAAC 
CTGGTTACGA CGTGTCTGCA GCGATGGAGG AATGAGATTG AGCAAGATGT GCGAGATCTG 
AAGGAGTCCA TTGCCAGAGT CAGCCTATCC ATTGACGAAA TGTACAGCGA CCCTCACCTC 
CAGCAGGTTC CTTATCACTT GCACGCTGTC TTGGTGCATG AAGGGCAAGC AAATGCTGGT 
CACTACTGGG CCTTCATATA TGACCAGCCT CGGAAAAGCT GGCTGAAATA CAATGACATC 
TCAGTGACAG AATCATCGTG GGAAGAGCTG GAGAGAGATT CATTTGGTGG CCTGAGGAAC 
GCCAGTGCTT ACTGCCTGAT GTACATAAGC GATAAGGTGT CCCACGTTGT TGCAGGTGAA 
GGTGACGGCT CAGAGGTCGG GCAGTTCCAG AAGGAGGTGG AGGCTTTGCC CCCAGAGCTG 
AGGCGTTACA TCCAGGAGGA CAACTGGCGG CTCGAGCAGG AGGCAGAAGA GTGGGAGGAG 
GAGCAGTCCT GCAAGATTCC TTCAACTGCT TCTGAATCTC AAGAGCTCTC TCCTGAGTCG 
GGGCTAGATC CACCTGCAGC CCACGAGCAG AGCCTGCGCT CTCTGTCCTC GGAGCACGCC 
ATGATTGCAA AGGAGCAGAC TGCTAAAGCC ATTGCCAACG CAGCCAACGT CTACGAGAAG 
AACGGCGTGG AGGCAGCGCT CTGCGAGGCA TTCCACGAGG AGTACTCCAG ACTCTATCTC 
CTTTCCAAAG AGACCCCGAC CCCTCAGAAC GATGCCCGCT TGCAACACGT GCTCGTTTAC 
TTCCTTCAGA ACGATGCTCC CCAGCAGATT GTGGAACGGA CCCTCCTCGA GCAGTTTGCA 
GACAAAAACC TCAGCTACGA TGAAAGGTCC ATTAGCATCA TGAAGGTAGC ACGAGATAAA 
CTGAAAGAGA TCGGTCCTGA TGAGGTCAAT ATGGAGGAGT ATAAGAAGTG GCATGAGGAC 
TACAGTCTTT TTCGGAAGGT GTCTGTTTAC CTGCTTACAG GGTTGGAGCT GTACCAGAAT 
AGAAAGTACA AGGAGTCCCT GACCTACCTG ATCTATGCCT ACCAAAGCAA CACCACTCTG 
CTGAAGAAGG GAGCCAACAG AGGAGTGAAT GAATCGCTGA TCACCTTGTA TAGAAGAAAG 
TGTCTCTTGA AGCTGAATGA GGTGGCATCA TCTCTGTTTG TAAGCTGTGA GGAAGCCCAT 
GTGGCAGAGG GCATTAGCAT CCTGAATGAG CTGATCATCC CCTGTATGCA CCTCATTAAC 
AACTTTGACA TCTCCAGAGA AGACATGGAT GCCATTGAGG TCATGAGAAA CCGCTGGTGC 
TCCTATCTGG GACGAGAAGA CATGGACGCA AGCCTGCAGA TCAGGCTGGG CGAGCTGCTC 
CCCCGCCTGC TGGACGGCTC CACGGAGGTG GTGGTGCTGA AGGAGCCCCC AAAGATCCGT 
CCCAACTCCC CATACGACCT CTGCAGCCGC TTTGCAGCGG TGATGGAGTC CATCCATGAC 
GCCTCGACTG TGACTGTGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	USP28
Entrez Gene ID	428246
Full Name	ubiquitin specific peptidase 28
General protein information	Preferred Names ubiquitin specific peptidase 28 Names ubiquitin carboxyl-terminal hydrolase 28 deubiquitinating enzyme 28 ubiquitin thioesterase 28 ubiquitin thiolesterase 28 ubiquitin-specific-processing protease 28
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Rattus norvegicus (Norway rat)	Usp28	NP_001101614.1
Danio rerio (zebrafish)	usp28	XP_001920096.1
Xenopus tropicalis (tropical clawed frog)	usp28	XP_004916150.1
Canis lupus familiaris (dog)	USP28	XP_005619813.1
Mus musculus (house mouse)	Usp28	NP_780691.2
Pan troglodytes (chimpanzee)	USP28	XP_003313357.2
	USP28	XP_002799842.1
Bos taurus (cattle)	USP28	NP_001179927.1
Homo sapiens (human)	USP28	NP_065937.1
Gallus gallus (chicken)	USP28	NP_001026667.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

USP28 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

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