LRRC8E cDNA ORF clone, Bos taurus(cattle)

The following LRRC8E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa21008 NM_001205622.1
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit E (LRRC8E), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21008 XM_010806808.3
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21008 XM_005208732.4
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21008 XM_024993992.1
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa21008 XM_005208732.3 Bos taurus leucine-rich repeat containing 8 family member E (LRRC8E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21008 XM_010806808.2 Bos taurus leucine-rich repeat containing 8 family member E (LRRC8E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa21008
    Clone ID Related Accession (Same CDS sequence) XM_010806808.2 , XM_024993992.1 , XM_005208732.4 , XM_005208732.3 , XM_010806808.3 , NM_001205622.1
    Accession Version NM_001205622.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8E
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On Apr 20, 2011 this sequence version replaced XM_584508.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CA034518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192551.1
    CDS79..2469
    Translation

    Target ORF information:

    RefSeq Version NM_001205622.1
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit E (LRRC8E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21008
    Clone ID Related Accession (Same CDS sequence) XM_010806808.2 , XM_024993992.1 , XM_005208732.4 , XM_005208732.3 , XM_010806808.3 , NM_001205622.1
    Accession Version XM_010806808.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805110.1
    CDS974..3364
    Translation

    Target ORF information:

    RefSeq Version XM_010806808.2
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine-rich repeat containing 8 family member E (LRRC8E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806808.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21008
    Clone ID Related Accession (Same CDS sequence) XM_010806808.2 , XM_024993992.1 , XM_005208732.4 , XM_005208732.3 , XM_010806808.3 , NM_001205622.1
    Accession Version XM_024993992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849760.1
    CDS413..2803
    Translation

    Target ORF information:

    RefSeq Version XM_024993992.1
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21008
    Clone ID Related Accession (Same CDS sequence) XM_010806808.2 , XM_024993992.1 , XM_005208732.4 , XM_005208732.3 , XM_010806808.3 , NM_001205622.1
    Accession Version XM_005208732.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208732.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208789.1
    CDS519..2909
    Translation

    Target ORF information:

    RefSeq Version XM_005208732.4
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208732.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21008
    Clone ID Related Accession (Same CDS sequence) XM_010806808.2 , XM_024993992.1 , XM_005208732.4 , XM_005208732.3 , XM_010806808.3 , NM_001205622.1
    Accession Version XM_005208732.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208789.1
    CDS956..3346
    Translation

    Target ORF information:

    RefSeq Version XM_005208732.3
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine-rich repeat containing 8 family member E (LRRC8E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208732.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21008
    Clone ID Related Accession (Same CDS sequence) XM_010806808.2 , XM_024993992.1 , XM_005208732.4 , XM_005208732.3 , XM_010806808.3 , NM_001205622.1
    Accession Version XM_010806808.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806808.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805110.1
    CDS537..2927
    Translation

    Target ORF information:

    RefSeq Version XM_010806808.3
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806808.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AACCCGCCTT CAAGGTGCTC 
    AAACCTTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTCCAGGT GACGCAGGAC AAGATCATCT GTCTGCCCAG TCATGAACCC
    CGGGAGAATT TATCTGAAGC CCCATGCCAA CAGCTGCTGC CGCGGGGGAT CTCAGAGCCC
    ATGGGGGACC TTCGGGAGTT GAGCGGCCTC AAGAACAACC TGGACCTACA GCAGTACAGC
    TTCATCAACC AGCTCTGCTA CGAGACGGCC CTCCACTGGT ATGCCAAGTA CTTTCCCTAC
    TTGGTTGTCA TCCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCC
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATTCTGG GCAAGTGTTT TGACTCGCCA
    TGGACTACTC GGGCGCTGTC CGAGGTCTCC GGGGAGAACC ACAAGGGCCC TGCTGCCACC
    ACAGCTGGGC GGGCGACAGT GACCGTGACC ACAACTGCAG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAGGTATT GCCAGAGCCC GAGAAGGTGG TGACAGAGCC CCCTGCTGTC
    ACCCTGCTGG ACAAGAAGGA GGGAGAGCAG GCCAAAGCCC TGTTTGAAAA GGTCAAGAAA
    TTCCGCGTGC ACGTGGAGGA GGGCGACATC CTGTACACCA TGTACATCCG GCAGACGGTG
    CTCAAGGTCT GCAAATTCTT GGCCATCCTG GTCTACAACC TGGTCTACGT GGGAAAGATC
    AGCTTCCTGG TGGCCTGCAG GGTGGAGACC TCGGAGGTGA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCTT TCTGCTACAT CTCCTTCGTG
    TGCGTCTACG GGATCACCTG TCTCTATACC CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGGTCAGT GCGGGAGGAA ACTGGCATGG GCGACATCCC CGATGTCAAG
    AACGACTTCG CCTTTATGCT GCACCTCATC GACCAGTACG ACTCGCTCTA CTCCAAGCGC
    TTTGCTGTCT TCCTCTCTGA GGTCAGCGAG AGCCGCCTGA AGCAGCTCAA CCTCAACCAC
    GAGTGGACTG CCGAGAAGCT GCGACAGAAG CTGCAGCGCA ACGCCCGGGG CCGGCTGGAA
    CTGGCCCTCT GCATGCTCCC GGGGCTGCCG GACACGGTCT TCGAGCTCAG CGAGGTGGAG
    GCGCTGCGGC TGGAGGCCAT TGGCGACATC ACCTTCCCCC CGGGCCTCTC GCAGCTGGTG
    CACTTGCAGG AGCTGAGCCT GCTCCACTCG CCCGCCAGGC TTCCGTTCTC CTCGCAGATC
    TTCCTGCGGG ACCGCCTGAA GGTCATCCGA ATCAAGTGCG AGGAGCTGCG GGAGGTGCCC
    CTCTGGGTGT TTGGGTTGCG TGGTCTGGAG GAGCTGCATC TAGAGGGGCT CTTTCCCCCA
    GAGCTGGCCC GGGCGGCCAC CCTCGAGAGC CTCCGGGAGC TGAAGCAGCT GAAGGTGCTG
    TCTCTGCGGA GCAACGCTGG CAAGGTTCCC GCCAGTGTGA CTGATGTGGC CGGCCACCTG
    CAGCGGCTCA GCCTGCACAA CGACGGGGCA CGTCTGTTGG CACTGAACAG CCTCAAGAAG
    CTGGCAGCGC TGCGGGAGCT GGAGCTGGTG GCCTGCGGAC TGGAGCGCAT CCCCCACGCT
    GTCTTCAGCC TAGGTGCGCT GCAGGAGCTT GACCTCCGGG ACAACCACCT GCGTTCCATC
    GAGGAGATCC TCAGCTTCCA GCACTGCCGC AAGCTGCTCA CACTCCGACT GTGGCACAAC
    CAGATTGCCT ATGTCCCCGA GCATGTGCGG AAGCTCCGGG GCCTCGAGCA GCTCTATCTG
    AGCCACAACA AGCTGGAGAC GCTGCCCACC CAGCTCGGCA TGTGCTCCAG CCTCCGCCTG
    CTGGACGTCT CGCACAATGG GCTGCATTCC CTGCCAGCCG AGCTGGGTCT CCTGCAGAAT
    CTTCAGCACT TGGCTCTCTC CTACAATGCC CTGGAGTTTC TACCCGATGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGCT GCTCCTAGGT TACAACCACC TGAGCCAGCT TGCGCCCCAG
    GTGGGGGCCC TCAGGGCCCT CAGCCGCCTG GAGCTCAAGG GCAACCGGCT GGAAGCACTG
    CCGGAAGAGC TCGGCAACTG TGGGGGGCTC AAGAAGTCGG GGCTCCTGGT GGAGGACACC
    CTTTATGATG GGCTTCCGGC CGAGGTACGG GACCGGATGG AGGCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.