LRRC8E cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRC8E
Entrez Gene ID	80131
Full Name	leucine rich repeat containing 8 VRAC subunit E
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit E Names volume-regulated anion channel subunit LRRC8E leucine rich repeat containing 8 family member E leucine-rich repeat-containing protein 8E
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 19 Map Location: 19p13.2
Summary	This gene encodes a member of a small, conserved family of proteins with similar structure, including a string of extracellular leucine-rich repeats. A related protein was shown to be involved in B-cell development. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012].
Disorder MIM:	612891

mRNA and Protein(s)

mRNA	Protein	Name
NM_001268284.2	NP_001255213.1	volume-regulated anion channel subunit LRRC8E isoform 1
NM_025061.6	NP_079337.2	volume-regulated anion channel subunit LRRC8E isoform 1
NM_025061.5	NP_079337.2	volume-regulated anion channel subunit LRRC8E isoform 1
NM_001268285.3	NP_001255214.1	volume-regulated anion channel subunit LRRC8E isoform 2
XM_011528319.2	XP_011526621.1	volume-regulated anion channel subunit LRRC8E isoform X1
NM_001268285.2	NP_001255214.1	volume-regulated anion channel subunit LRRC8E isoform 2
XM_011528319.1	XP_011526621.1	volume-regulated anion channel subunit LRRC8E isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8E	NP_001255213.1
Pan troglodytes (chimpanzee)	LRRC8E	XP_003953389.1
Canis lupus familiaris (dog)	LRRC8E	XP_005633001.1
Bos taurus (cattle)	LRRC8E	NP_001192551.1
Rattus norvegicus (Norway rat)	Lrrc8e	NP_001029311.1
Xenopus tropicalis (tropical clawed frog)	lrrc8e	NP_001007863.1
	LRRC8E	XP_001093244.1
Mus musculus (house mouse)	Lrrc8e	NP_082451.2

Gene Ontology
Bibliography

Related articles in PubMed

Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels.
Lutter D, Ullrich F, Lueck JC, Kempa S, Jentsch TJ
Journal of cell science130(6)1122-1133(2017 03)

LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense Ionic Strength.
Syeda R, Qiu Z, Dubin AE, Murthy SE, Florendo MN, Mason DE, Mathur J, Cahalan SM, Peters EC, Montal M, Patapoutian A
Cell164(3)499-511(2016 Jan)

Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC.
Voss FK, Ullrich F, M?nch J, Lazarow K, Lutter D, Mah N, Andrade-Navarro MA, von Kries JP, Stauber T, Jentsch TJ
Science (New York, N.Y.)344(6184)634-8(2014 May)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins.
Kubota K, Kim JY, Sawada A, Tokimasa S, Fujisaki H, Matsuda-Hashii Y, Ozono K, Hara J
FEBS letters564(1-2)147-52(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified four genes, named TA-LRRP, AD158, LRRC5, and FLJ23420, as unknown LRRC8-like genes
Title: LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins.

The following LRRC8E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00041	NM_025061.6 Latest version!	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu00041	NM_001268284.2 Latest version!	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu29088	NM_001268285.3 Latest version!	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu00041	XM_011528319.2 Latest version!	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu00041	NM_025061.5	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu29088	NM_001268285.2	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu00041	XM_011528319.1	Homo sapiens leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRC8E ( NM_001268284.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001255213.1 Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00041
Clone ID Related Accession (Same CDS sequence)	NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
Accession Version	NM_001268284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	volume-regulated anion channel subunit LRRC8E isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, CF593782.1, KF459574.1, BX538180.1, AC010336.8 and CB049178.1. This sequence is a reference standard in the RefSeqGene project. On Jan 17, 2014 this sequence version replaced NM_001268284.1. Transcript Variant: This variant (2) contains an additional exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: CF593782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569, SAMN02400288 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255213.1
CDS	245..2635
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPNHELQENLSEAPCQQLLPRGIPEQIGALQEVKGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLELALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDTLYQGLPAEVRDKMEEE

Target ORF information:

RefSeq Version	NM_001268284.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268284.2

ORF Insert Sequence:

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( NM_025061.6 ) cDNA ORF clone, Homo sapiens(Human) -> NP_079337.2 Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00041
Clone ID Related Accession (Same CDS sequence)	NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
Accession Version	NM_025061.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	volume-regulated anion channel subunit LRRC8E isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, KF459574.1, BX538180.1 and CB049178.1. On Nov 22, 2018 this sequence version replaced NM_025061.5. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: BX538180.1, BC070089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000306708.11/ ENSP00000306524.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079337.2
CDS	97..2487
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPNHELQENLSEAPCQQLLPRGIPEQIGALQEVKGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLELALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDTLYQGLPAEVRDKMEEE

Target ORF information:

RefSeq Version	NM_025061.6
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025061.6

ORF Insert Sequence:

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( NM_025061.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_079337.2 Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA.

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CloneID	OHu00041
Clone ID Related Accession (Same CDS sequence)	NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
Accession Version	NM_025061.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	volume-regulated anion channel subunit LRRC8E isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, KF459574.1, BX538180.1 and CB049178.1. On Jan 17, 2014 this sequence version replaced NM_025061.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: BC070089.1, BX538180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079337.2
CDS	102..2492
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPNHELQENLSEAPCQQLLPRGIPEQIGALQEVKGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLELALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDTLYQGLPAEVRDKMEEE

Target ORF information:

RefSeq Version	NM_025061.5
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025061.5

ORF Insert Sequence:

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( NM_001268285.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001255214.1 Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA.

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CloneID	OHu29088
Clone ID Related Accession (Same CDS sequence)	NM_001268285.3 , NM_001268285.2
Accession Version	NM_001268285.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	volume-regulated anion channel subunit LRRC8E isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, DA447942.1, KF459574.1, BX538180.1, AC010336.8 and CB049178.1. On May 31, 2019 this sequence version replaced NM_001268285.2. Transcript Variant: This variant (3) lacks an exon in the 5' coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR6380202.129487.3, SRR6380202.129487.11 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTGCA CCAGTTTCTG GTTCAAGTTC CCTGGCACCA GCTCCAAGAT TGAACACTTC 
ATCTCCATCC TGGGCAAGTG TTTCGACTCT CCATGGACCA CCAGGGCCCT ATCCGAGGTC 
TCCGGGGAGA ACCAGAAGGG CCCAGCAGCC ACCGAACGGG CTGCGGCCAC CATAGTGGCC 
ATGGCAGGGA CCGGGCCGGG GAAGGCAGGG GAGGGTGAGA AGGAGAAAGT GCTGGCGGAA 
CCGGAGAAGG TGGTGACCGA GCCTCCAGTT GTCACCCTGT TGGACAAGAA GGAGGGTGAG 
CAAGCCAAAG CCCTGTTTGA GAAGGTGAAG AAGTTCCGCA TGCACGTGGA AGAGGGCGAC 
ATCCTGTACA CCATGTACAT CCGACAGACG GTGCTGAAAG TGTGTAAGTT CCTGGCCATC 
CTGGTCTACA ACCTGGTCTA TGTGGAGAAG ATCAGTTTCC TGGTGGCCTG TAGGGTGGAG 
ACGTCAGAGG TCACGGGCTA CGCCAGCTTC TGCTGCAACC ACACCAAGGC CCACCTCTTC 
TCCAAGCTGG CCTTCTGTTA CATCTCCTTT GTGTGCATCT ACGGACTTAC CTGCATCTAC 
ACGCTCTACT GGCTCTTCCA CCGGCCCCTC AAGGAGTACT CCTTCCGTTC CGTGCGGGAG 
GAGACTGGCA TGGGGGACAT TCCTGACGTC AAGAATGACT TCGCCTTCAT GCTGCACCTC 
ATCGATCAGT ACGACTCCCT CTACTCCAAG CGCTTCGCCG TCTTCCTGTC CGAGGTCAGC 
GAAAGCCGTC TAAAGCAGCT CAATCTCAAC CACGAGTGGA CGCCCGAGAA GCTTCGACAG 
AAGCTGCAGC GCAATGCCGC GGGCCGGCTG GAGCTGGCCC TCTGCATGCT GCCGGGTCTG 
CCCGACACCG TCTTTGAGCT CAGTGAGGTG GAGTCACTCA GGCTGGAGGC CATCTGCGAT 
ATCACCTTCC CCCCGGGGCT GTCACAGCTG GTGCACTTGC AGGAGCTCAG CTTGCTCCAC 
TCGCCCGCCA GGCTACCCTT CTCCTTGCAG GTCTTCCTGC GGGACCACCT GAAGGTGATG 
CGCGTCAAAT GCGAGGAGCT CCGCGAGGTG CCGCTTTGGG TGTTTGGGCT GCGGGGCTTG 
GAGGAGCTGC ACCTGGAGGG GCTTTTCCCC CAGGAGCTAG CTCGGGCAGC CACCCTGGAG 
AGCCTCCGGG AGCTGAAGCA GCTCAAGGTG TTGTCCCTCC GGAGCAACGC CGGGAAGGTG 
CCAGCCAGTG TGACCGACGT TGCTGGCCAC CTGCAGAGGC TCAGCCTGCA CAACGATGGG 
GCCCGTCTGG TTGCCCTGAA CAGCCTCAAG AAGCTGGCGG CATTGCGGGA GCTGGAGCTG 
GTGGCCTGCG GGCTGGAGCG CATCCCCCAT GCAGTGTTCA GCCTGGGTGC GCTGCAGGAA 
CTTGACCTCA AGGACAACCA CCTGCGCTCC ATCGAGGAAA TCCTCAGCTT CCAGCACTGC 
CGGAAGCTGG TCACGCTCAG GCTGTGGCAC AACCAGATCG CCTACGTCCC TGAGCACGTG 
CGGAAGCTCA GGAGCCTGGA GCAGCTCTAC CTCAGCTACA ACAAGCTGGA GACCCTGCCC 
TCCCAGCTCG GCCTGTGCTC AGGCCTCCGT CTGCTGGATG TGTCCCACAA TGGGCTACAC 
TCCCTGCCAC CCGAGGTGGG CCTCCTGCAG AACCTACAGC ACCTGGCCCT CTCCTACAAT 
GCCCTGGAGG CCCTGCCCGA AGAGCTCTTC TTCTGCCGCA AGCTGCGGAC GTTGCTTCTG 
GGCGACAACC AGCTGAGCCA GCTCTCGCCC CACGTGGGTG CCCTCAGAGC CCTCAGCCGC 
CTGGAGCTCA AAGGCAACCG CTTAGAGGCG CTGCCAGAAG AACTTGGCAA CTGTGGGGGG 
CTCAAGAAGG CGGGGCTCCT GGTGGAAGAC ACGCTTTACC AGGGTCTGCC GGCAGAAGTG 
CGGGACAAGA TGGAGGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255214.1
CDS	341..2344
Translation	MVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLELALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDTLYQGLPAEVRDKMEEE

Target ORF information:

RefSeq Version	NM_001268285.3
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268285.3

ORF Insert Sequence:

ATGGTCTGCA CCAGTTTCTG GTTCAAGTTC CCTGGCACCA GCTCCAAGAT TGAACACTTC 
ATCTCCATCC TGGGCAAGTG TTTCGACTCT CCATGGACCA CCAGGGCCCT ATCCGAGGTC 
TCCGGGGAGA ACCAGAAGGG CCCAGCAGCC ACCGAACGGG CTGCGGCCAC CATAGTGGCC 
ATGGCAGGGA CCGGGCCGGG GAAGGCAGGG GAGGGTGAGA AGGAGAAAGT GCTGGCGGAA 
CCGGAGAAGG TGGTGACCGA GCCTCCAGTT GTCACCCTGT TGGACAAGAA GGAGGGTGAG 
CAAGCCAAAG CCCTGTTTGA GAAGGTGAAG AAGTTCCGCA TGCACGTGGA AGAGGGCGAC 
ATCCTGTACA CCATGTACAT CCGACAGACG GTGCTGAAAG TGTGTAAGTT CCTGGCCATC 
CTGGTCTACA ACCTGGTCTA TGTGGAGAAG ATCAGTTTCC TGGTGGCCTG TAGGGTGGAG 
ACGTCAGAGG TCACGGGCTA CGCCAGCTTC TGCTGCAACC ACACCAAGGC CCACCTCTTC 
TCCAAGCTGG CCTTCTGTTA CATCTCCTTT GTGTGCATCT ACGGACTTAC CTGCATCTAC 
ACGCTCTACT GGCTCTTCCA CCGGCCCCTC AAGGAGTACT CCTTCCGTTC CGTGCGGGAG 
GAGACTGGCA TGGGGGACAT TCCTGACGTC AAGAATGACT TCGCCTTCAT GCTGCACCTC 
ATCGATCAGT ACGACTCCCT CTACTCCAAG CGCTTCGCCG TCTTCCTGTC CGAGGTCAGC 
GAAAGCCGTC TAAAGCAGCT CAATCTCAAC CACGAGTGGA CGCCCGAGAA GCTTCGACAG 
AAGCTGCAGC GCAATGCCGC GGGCCGGCTG GAGCTGGCCC TCTGCATGCT GCCGGGTCTG 
CCCGACACCG TCTTTGAGCT CAGTGAGGTG GAGTCACTCA GGCTGGAGGC CATCTGCGAT 
ATCACCTTCC CCCCGGGGCT GTCACAGCTG GTGCACTTGC AGGAGCTCAG CTTGCTCCAC 
TCGCCCGCCA GGCTACCCTT CTCCTTGCAG GTCTTCCTGC GGGACCACCT GAAGGTGATG 
CGCGTCAAAT GCGAGGAGCT CCGCGAGGTG CCGCTTTGGG TGTTTGGGCT GCGGGGCTTG 
GAGGAGCTGC ACCTGGAGGG GCTTTTCCCC CAGGAGCTAG CTCGGGCAGC CACCCTGGAG 
AGCCTCCGGG AGCTGAAGCA GCTCAAGGTG TTGTCCCTCC GGAGCAACGC CGGGAAGGTG 
CCAGCCAGTG TGACCGACGT TGCTGGCCAC CTGCAGAGGC TCAGCCTGCA CAACGATGGG 
GCCCGTCTGG TTGCCCTGAA CAGCCTCAAG AAGCTGGCGG CATTGCGGGA GCTGGAGCTG 
GTGGCCTGCG GGCTGGAGCG CATCCCCCAT GCAGTGTTCA GCCTGGGTGC GCTGCAGGAA 
CTTGACCTCA AGGACAACCA CCTGCGCTCC ATCGAGGAAA TCCTCAGCTT CCAGCACTGC 
CGGAAGCTGG TCACGCTCAG GCTGTGGCAC AACCAGATCG CCTACGTCCC TGAGCACGTG 
CGGAAGCTCA GGAGCCTGGA GCAGCTCTAC CTCAGCTACA ACAAGCTGGA GACCCTGCCC 
TCCCAGCTCG GCCTGTGCTC AGGCCTCCGT CTGCTGGATG TGTCCCACAA TGGGCTACAC 
TCCCTGCCAC CCGAGGTGGG CCTCCTGCAG AACCTACAGC ACCTGGCCCT CTCCTACAAT 
GCCCTGGAGG CCCTGCCCGA AGAGCTCTTC TTCTGCCGCA AGCTGCGGAC GTTGCTTCTG 
GGCGACAACC AGCTGAGCCA GCTCTCGCCC CACGTGGGTG CCCTCAGAGC CCTCAGCCGC 
CTGGAGCTCA AAGGCAACCG CTTAGAGGCG CTGCCAGAAG AACTTGGCAA CTGTGGGGGG 
CTCAAGAAGG CGGGGCTCCT GGTGGAAGAC ACGCTTTACC AGGGTCTGCC GGCAGAAGTG 
CGGGACAAGA TGGAGGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( NM_001268285.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001255214.1 Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA.

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CloneID	OHu29088
Clone ID Related Accession (Same CDS sequence)	NM_001268285.3 , NM_001268285.2
Accession Version	NM_001268285.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	volume-regulated anion channel subunit LRRC8E isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, DA447942.1, KF459574.1, BX538180.1, AC010336.8 and CB049178.1. On Jan 17, 2014 this sequence version replaced NM_001268285.1. Transcript Variant: This variant (3) lacks an exon in the 5' coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: DA447942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTGCA CCAGTTTCTG GTTCAAGTTC CCTGGCACCA GCTCCAAGAT TGAACACTTC 
ATCTCCATCC TGGGCAAGTG TTTCGACTCT CCATGGACCA CCAGGGCCCT ATCCGAGGTC 
TCCGGGGAGA ACCAGAAGGG CCCAGCAGCC ACCGAACGGG CTGCGGCCAC CATAGTGGCC 
ATGGCAGGGA CCGGGCCGGG GAAGGCAGGG GAGGGTGAGA AGGAGAAAGT GCTGGCGGAA 
CCGGAGAAGG TGGTGACCGA GCCTCCAGTT GTCACCCTGT TGGACAAGAA GGAGGGTGAG 
CAAGCCAAAG CCCTGTTTGA GAAGGTGAAG AAGTTCCGCA TGCACGTGGA AGAGGGCGAC 
ATCCTGTACA CCATGTACAT CCGACAGACG GTGCTGAAAG TGTGTAAGTT CCTGGCCATC 
CTGGTCTACA ACCTGGTCTA TGTGGAGAAG ATCAGTTTCC TGGTGGCCTG TAGGGTGGAG 
ACGTCAGAGG TCACGGGCTA CGCCAGCTTC TGCTGCAACC ACACCAAGGC CCACCTCTTC 
TCCAAGCTGG CCTTCTGTTA CATCTCCTTT GTGTGCATCT ACGGACTTAC CTGCATCTAC 
ACGCTCTACT GGCTCTTCCA CCGGCCCCTC AAGGAGTACT CCTTCCGTTC CGTGCGGGAG 
GAGACTGGCA TGGGGGACAT TCCTGACGTC AAGAATGACT TCGCCTTCAT GCTGCACCTC 
ATCGATCAGT ACGACTCCCT CTACTCCAAG CGCTTCGCCG TCTTCCTGTC CGAGGTCAGC 
GAAAGCCGTC TAAAGCAGCT CAATCTCAAC CACGAGTGGA CGCCCGAGAA GCTTCGACAG 
AAGCTGCAGC GCAATGCCGC GGGCCGGCTG GAGCTGGCCC TCTGCATGCT GCCGGGTCTG 
CCCGACACCG TCTTTGAGCT CAGTGAGGTG GAGTCACTCA GGCTGGAGGC CATCTGCGAT 
ATCACCTTCC CCCCGGGGCT GTCACAGCTG GTGCACTTGC AGGAGCTCAG CTTGCTCCAC 
TCGCCCGCCA GGCTACCCTT CTCCTTGCAG GTCTTCCTGC GGGACCACCT GAAGGTGATG 
CGCGTCAAAT GCGAGGAGCT CCGCGAGGTG CCGCTTTGGG TGTTTGGGCT GCGGGGCTTG 
GAGGAGCTGC ACCTGGAGGG GCTTTTCCCC CAGGAGCTAG CTCGGGCAGC CACCCTGGAG 
AGCCTCCGGG AGCTGAAGCA GCTCAAGGTG TTGTCCCTCC GGAGCAACGC CGGGAAGGTG 
CCAGCCAGTG TGACCGACGT TGCTGGCCAC CTGCAGAGGC TCAGCCTGCA CAACGATGGG 
GCCCGTCTGG TTGCCCTGAA CAGCCTCAAG AAGCTGGCGG CATTGCGGGA GCTGGAGCTG 
GTGGCCTGCG GGCTGGAGCG CATCCCCCAT GCAGTGTTCA GCCTGGGTGC GCTGCAGGAA 
CTTGACCTCA AGGACAACCA CCTGCGCTCC ATCGAGGAAA TCCTCAGCTT CCAGCACTGC 
CGGAAGCTGG TCACGCTCAG GCTGTGGCAC AACCAGATCG CCTACGTCCC TGAGCACGTG 
CGGAAGCTCA GGAGCCTGGA GCAGCTCTAC CTCAGCTACA ACAAGCTGGA GACCCTGCCC 
TCCCAGCTCG GCCTGTGCTC AGGCCTCCGT CTGCTGGATG TGTCCCACAA TGGGCTACAC 
TCCCTGCCAC CCGAGGTGGG CCTCCTGCAG AACCTACAGC ACCTGGCCCT CTCCTACAAT 
GCCCTGGAGG CCCTGCCCGA AGAGCTCTTC TTCTGCCGCA AGCTGCGGAC GTTGCTTCTG 
GGCGACAACC AGCTGAGCCA GCTCTCGCCC CACGTGGGTG CCCTCAGAGC CCTCAGCCGC 
CTGGAGCTCA AAGGCAACCG CTTAGAGGCG CTGCCAGAAG AACTTGGCAA CTGTGGGGGG 
CTCAAGAAGG CGGGGCTCCT GGTGGAAGAC ACGCTTTACC AGGGTCTGCC GGCAGAAGTG 
CGGGACAAGA TGGAGGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255214.1
CDS	346..2349
Translation	MVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLELALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDTLYQGLPAEVRDKMEEE

Target ORF information:

RefSeq Version	NM_001268285.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268285.2

ORF Insert Sequence:

ATGGTCTGCA CCAGTTTCTG GTTCAAGTTC CCTGGCACCA GCTCCAAGAT TGAACACTTC 
ATCTCCATCC TGGGCAAGTG TTTCGACTCT CCATGGACCA CCAGGGCCCT ATCCGAGGTC 
TCCGGGGAGA ACCAGAAGGG CCCAGCAGCC ACCGAACGGG CTGCGGCCAC CATAGTGGCC 
ATGGCAGGGA CCGGGCCGGG GAAGGCAGGG GAGGGTGAGA AGGAGAAAGT GCTGGCGGAA 
CCGGAGAAGG TGGTGACCGA GCCTCCAGTT GTCACCCTGT TGGACAAGAA GGAGGGTGAG 
CAAGCCAAAG CCCTGTTTGA GAAGGTGAAG AAGTTCCGCA TGCACGTGGA AGAGGGCGAC 
ATCCTGTACA CCATGTACAT CCGACAGACG GTGCTGAAAG TGTGTAAGTT CCTGGCCATC 
CTGGTCTACA ACCTGGTCTA TGTGGAGAAG ATCAGTTTCC TGGTGGCCTG TAGGGTGGAG 
ACGTCAGAGG TCACGGGCTA CGCCAGCTTC TGCTGCAACC ACACCAAGGC CCACCTCTTC 
TCCAAGCTGG CCTTCTGTTA CATCTCCTTT GTGTGCATCT ACGGACTTAC CTGCATCTAC 
ACGCTCTACT GGCTCTTCCA CCGGCCCCTC AAGGAGTACT CCTTCCGTTC CGTGCGGGAG 
GAGACTGGCA TGGGGGACAT TCCTGACGTC AAGAATGACT TCGCCTTCAT GCTGCACCTC 
ATCGATCAGT ACGACTCCCT CTACTCCAAG CGCTTCGCCG TCTTCCTGTC CGAGGTCAGC 
GAAAGCCGTC TAAAGCAGCT CAATCTCAAC CACGAGTGGA CGCCCGAGAA GCTTCGACAG 
AAGCTGCAGC GCAATGCCGC GGGCCGGCTG GAGCTGGCCC TCTGCATGCT GCCGGGTCTG 
CCCGACACCG TCTTTGAGCT CAGTGAGGTG GAGTCACTCA GGCTGGAGGC CATCTGCGAT 
ATCACCTTCC CCCCGGGGCT GTCACAGCTG GTGCACTTGC AGGAGCTCAG CTTGCTCCAC 
TCGCCCGCCA GGCTACCCTT CTCCTTGCAG GTCTTCCTGC GGGACCACCT GAAGGTGATG 
CGCGTCAAAT GCGAGGAGCT CCGCGAGGTG CCGCTTTGGG TGTTTGGGCT GCGGGGCTTG 
GAGGAGCTGC ACCTGGAGGG GCTTTTCCCC CAGGAGCTAG CTCGGGCAGC CACCCTGGAG 
AGCCTCCGGG AGCTGAAGCA GCTCAAGGTG TTGTCCCTCC GGAGCAACGC CGGGAAGGTG 
CCAGCCAGTG TGACCGACGT TGCTGGCCAC CTGCAGAGGC TCAGCCTGCA CAACGATGGG 
GCCCGTCTGG TTGCCCTGAA CAGCCTCAAG AAGCTGGCGG CATTGCGGGA GCTGGAGCTG 
GTGGCCTGCG GGCTGGAGCG CATCCCCCAT GCAGTGTTCA GCCTGGGTGC GCTGCAGGAA 
CTTGACCTCA AGGACAACCA CCTGCGCTCC ATCGAGGAAA TCCTCAGCTT CCAGCACTGC 
CGGAAGCTGG TCACGCTCAG GCTGTGGCAC AACCAGATCG CCTACGTCCC TGAGCACGTG 
CGGAAGCTCA GGAGCCTGGA GCAGCTCTAC CTCAGCTACA ACAAGCTGGA GACCCTGCCC 
TCCCAGCTCG GCCTGTGCTC AGGCCTCCGT CTGCTGGATG TGTCCCACAA TGGGCTACAC 
TCCCTGCCAC CCGAGGTGGG CCTCCTGCAG AACCTACAGC ACCTGGCCCT CTCCTACAAT 
GCCCTGGAGG CCCTGCCCGA AGAGCTCTTC TTCTGCCGCA AGCTGCGGAC GTTGCTTCTG 
GGCGACAACC AGCTGAGCCA GCTCTCGCCC CACGTGGGTG CCCTCAGAGC CCTCAGCCGC 
CTGGAGCTCA AAGGCAACCG CTTAGAGGCG CTGCCAGAAG AACTTGGCAA CTGTGGGGGG 
CTCAAGAAGG CGGGGCTCCT GGTGGAAGAC ACGCTTTACC AGGGTCTGCC GGCAGAAGTG 
CGGGACAAGA TGGAGGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( XM_011528319.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011526621.1 Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.

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CloneID	OHu00041
Clone ID Related Accession (Same CDS sequence)	NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
Accession Version	XM_011528319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	volume-regulated anion channel subunit LRRC8E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526621.1
CDS	830..3220
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPNHELQENLSEAPCQQLLPRGIPEQIGALQEVKGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLELALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDTLYQGLPAEVRDKMEEE

Target ORF information:

RefSeq Version	XM_011528319.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528319.2

ORF Insert Sequence:

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( XM_011528319.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011526621.1 Homo sapiens leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu00041
Clone ID Related Accession (Same CDS sequence)	NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
Accession Version	XM_011528319.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	volume-regulated anion channel subunit LRRC8E isoform X1
Comment

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011526621.1

Translation

MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPNHELQENLSEAPCQQLLPRGIPEQIGALQEVKGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLELALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDTLYQGLPAEVRDKMEEE

Target ORF information:

RefSeq Version	XM_011528319.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528319.1

ORF Insert Sequence:

ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC 
TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC 
CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG 
ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC 
TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC 
CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA 
TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC 
GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG 
GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC 
ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG 
TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG 
CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC 
AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC 
TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG 
TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG 
GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG 
AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC 
TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC 
GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG 
CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG 
TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG 
CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC 
TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG 
CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG 
GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG 
TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG 
CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG 
CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA 
GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC 
GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC 
CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC 
AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG 
CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC 
CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC 
TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC 
GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG 
CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG 
CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels.
Journal of cell science130(6)1122-1133(2017 03)
Lutter D,Ullrich F,Lueck JC,Kempa S,Jentsch TJ

LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense Ionic Strength.
Cell164(3)499-511(2016 Jan)
Syeda R,Qiu Z,Dubin AE,Murthy SE,Florendo MN,Mason DE,Mathur J,Cahalan SM,Peters EC,Montal M,Patapoutian A

Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC.
Science (New York, N.Y.)344(6184)634-8(2014 May)
Voss FK,Ullrich F,M?nch J,Lazarow K,Lutter D,Mah N,Andrade-Navarro MA,von Kries JP,Stauber T,Jentsch TJ

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins.
FEBS letters564(1-2)147-52(2004 Apr)
Kubota K,Kim JY,Sawada A,Tokimasa S,Fujisaki H,Matsuda-Hashii Y,Ozono K,Hara J

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LRRC8E cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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