LRRC8E cDNA ORF clone, Canis lupus familiaris(dog)

The following LRRC8E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe22259 XM_005632944.2
Latest version!
Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe22259 XM_005632945.3
Latest version!
Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe22259 XM_542121.6
Latest version!
Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OCe22259 XM_005632945.2 Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe22259 XM_005632944.1 Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe22259 XM_542121.5 Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe22259
    Clone ID Related Accession (Same CDS sequence) XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
    Accession Version XM_005632945.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product volume-regulated anion channel subunit LRRC8E
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005632945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633002.1
    CDS99..2480
    Translation

    Target ORF information:

    RefSeq Version XM_005632945.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005632945.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe22259
    Clone ID Related Accession (Same CDS sequence) XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
    Accession Version XM_005632945.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product volume-regulated anion channel subunit LRRC8E
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005632945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633002.1
    CDS396..2777
    Translation

    Target ORF information:

    RefSeq Version XM_005632945.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005632945.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe22259
    Clone ID Related Accession (Same CDS sequence) XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
    Accession Version XM_005632944.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product volume-regulated anion channel subunit LRRC8E
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005632944.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633001.1
    CDS346..2727
    Translation

    Target ORF information:

    RefSeq Version XM_005632944.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005632944.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe22259
    Clone ID Related Accession (Same CDS sequence) XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
    Accession Version XM_005632944.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product volume-regulated anion channel subunit LRRC8E
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633001.1
    CDS51..2432
    Translation

    Target ORF information:

    RefSeq Version XM_005632944.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005632944.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe22259
    Clone ID Related Accession (Same CDS sequence) XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
    Accession Version XM_542121.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product volume-regulated anion channel subunit LRRC8E
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_542121.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_542121.2
    CDS74..2455
    Translation

    Target ORF information:

    RefSeq Version XM_542121.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_542121.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe22259
    Clone ID Related Accession (Same CDS sequence) XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
    Accession Version XM_542121.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product volume-regulated anion channel subunit LRRC8E
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_542121.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_542121.2
    CDS91..2472
    Translation

    Target ORF information:

    RefSeq Version XM_542121.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_542121.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC
    TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC
    CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG
    ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC
    TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC
    CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA
    TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA
    CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG
    GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG
    GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG
    CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC
    TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG
    GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC
    ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT
    GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC
    TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT
    GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC
    TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG
    CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC
    TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG
    CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG
    GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG
    GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG
    TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT
    CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG
    AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT
    AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA
    CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC
    CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC
    CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT
    TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC
    AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC
    TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC
    CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG
    CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC
    CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG
    CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG
    GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.