TMEM67 cDNA ORF clone, Bos taurus(cattle)

The following TMEM67 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM67 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01775 NM_001205299.1
Latest version!
Bos taurus transmembrane protein 67 (TMEM67), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa222221 XM_025001457.1
Latest version!
Bos taurus transmembrane protein 67 (TMEM67), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222222 XM_025001458.1
Latest version!
Bos taurus transmembrane protein 67 (TMEM67), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222223 XM_025001459.1
Latest version!
Bos taurus transmembrane protein 67 (TMEM67), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa01775
    Clone ID Related Accession (Same CDS sequence) NM_001205299.1
    Accession Version NM_001205299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product meckelin precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000014.1, CK775752.1 and CF931023.1. On or before Apr 14, 2011 this sequence version replaced XM_002692841.1, XM_583257.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.28691.1, SRR5571254.38213.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACGG GAGGCCGCGG GGCTGTGGTT GCGATGGCCG CTGGGTCCAC CCTGTCGTCT 
    GCGAGTGTGA CTCTGTTCCT CTCGCTGGTC CTCCCTCGCG TCTCACAAGC TCAGACCTTC
    TTTTTCCCTT TCCGGCAGCC GGAGACGTGC GGCCACAACC AGTACTTCGA TATCTCGGCG
    CTCTCCTGTG TGGCGTGTGG TTCCAATCAG AGGCAGGATG CCGGAGGAAC TTCATGTGTG
    TGTCTACCAG GATTTCAGAT GATTTCTAAT AATGGAGGAC CTGATATTAC TTGTAAAAAG
    TGCCCAGAAC ACATGAAAGG TGTTACTGAA GATGGCTGGA ACTGTATTTC TTGCCCTGGT
    GGTTTAACTG CAGAAGGAAA ATGCCACTGT CCCACTGGCC ATATTTTAGT GGAAAGAAAT
    GTTAATGGAA CATTGTTGTC TCAAGCAACT TGTGAGCTCT GTGATGAAAG TGAAAACTCT
    TTTACAGTAG CAAATGCTTT AGGAAACAGG TGTGTCCGAT GTGAACCAGC GTTCATTAAT
    ACCAGCAAGT CCTGTGCATG TTCAGAACCT AACATTTTAA CAGGGGGATT GTGTTTGAGC
    AGCACAGGGA ATTTTCCTCT ACGTATGATT TCAACTGCAC GTTATGGAGA GCTTGGCATA
    TCTTTCACTT CAGAATGGTT TGCAAAGTAT TTGCAGTCAT CAGCAGCTGC TTGTTGGGTG
    TATGGCAATC TAACATCTTG CCAAGCTCTC GGAAATATGT GTGTAATGAA CATGAACTAT
    TATGACTCCT CTACTTTTGA TGCATGTGGA TTGTTTCAGT TTATCTTTGA AAATACTGCT
    GCACTGGGCA CTGTTCATTC TGTCTCCTTT TGGAGACAGA ATCTTCCATG GCTGTTCTAT
    GGAGACCAGC TAGGATTAGC ACCTCAAATA CTTAGTACTA CACCTCTTCC TACAAATTTC
    AGTTTTAAAG GGGAAAACCA GAGTACAAAA CTGAAGTTTG TTGCTGCTTC CTATGATGTG
    AGAGGAAATT TTCTCAAGTG GCAAACTTTA GAAGGAGGGA TTTTACAGCT TTGTCCAGAC
    ACAGAGACGA GACTAAATGC TGCTTATTCC TTTGGAACAA CTTATCAACA AAGTTGTGAG
    ATTCCTGTCC CTAAGATCTT AACTGACTTT CCCACTCCTA CGTTTTATGA TGTGTACCTT
    GAATATACTG ATGAGAATCA GCACCAATAT ATTTGGGCTG TGCCTGTGTT AAACTTAAAT
    CTTCAGCACA AGAAAATGTT TGTGAACAAA GACAGTAGTT CTGGCAAGTG GCTTCTGACT
    CGGCGCATTT TCTTAGTGGA TACACTAAGT GGACGGGAAA ATGACTTAGG AAGCCAGCCA
    AGATTAATTA GAGTCGCCAC CCAAATATCA CTGAGCATCC ACCTTGTACC CAACACAAAA
    AATGGAAACA TTTACCCTCC CTTAATTACC ATTGCCTACC ATGACATTGA TATCAAAGAT
    CCCAGCAGCC AGTCTGTGAA GGTTTCTTTC TCAGTCACAT ACGAAATGGA TCAAGGAGAA
    GCACATGTCC AGACAGATAT TGCTTTAGGT GTATTGGGTG GACTAGCTGT TTTATCATCT
    CTTTTGAAGA CAGCAGGTTG GAAGAGACGT ATTGGGAGTC CCATGATTGA TTTACAGACA
    GTTGCGAAAT TCTTGGTTTA CTATGCTGGT GATCTGGCCA ATGTTTTCTT TATCATCACT
    GTAGGAACAG GTCTTTACTG GCTTATTTTC TTTAAAGCAC AGAAGTCTGT CTCTGTTTTG
    CTGCCAGTGC CAGCTCAAGA AAAACGTTTT GTTACTTATG TGGGATGTGG TTTTGCTCTG
    AAGGCTCTAC AATTTTTGCA TAAGCTCATC TCCCAGATTA CCATAGACAT ATTCTTTATC
    GATTGGGAGC GGCCTAAAGG AAAGGTTCTT AAAGCTGTTG AAGGTGAGGG TGGTGTTCGG
    AGTGCTACTG TTCCTGTAAG CATATGGAGA ACTTATTTTG TGGCAAATGA ATGGAACGAA
    ATTCAGACTG TGAGAAAAAT TAACCCACTC TTTCAAGTAC TTACTGTCCT CTTCCTTTTG
    GAGGTTGTGG GATTTAAGAA CTTAGCATTA ATGGACTCAT CCTCTAGTCT TTCCAGAAGC
    CCACCTAGCT ACATAGCTCC TTACAGCCGC ATTTTGAGAT ACGCGGTATC TTCTGCTCTT
    TGGCTGGTCA TTGGACTTAT ACAGATTGTG TTCTTTGCCG TCTTCTATGA GAGATTTATA
    GAAGATAAAA TTCGACAGTT TGTTGATTTG TGCTGTATGA GTAATATATC AGTGTTTCTG
    TTATCCCACA GATGTTTTGG CTACTACATC CATGGTAGAT CAGTACATGG GCATGCTGAT
    ACTAATATGG AAGAAATGAA TATGAATCTT AAAAGAGAAG CGGAAAATTT ATGTAGCCAA
    AGAGGTTTGG TACCTAATAC AGATGGTCAG ACTTTTCAGA TTGCAATTTC TAGCCAGATG
    AGGCAACACT ATGACAGAAT TCATGAGACA CTAACAAGGA AAAATGGCCC TGCTAGACTA
    TTGAGTTCCT CAGGAAGTAC TTTCGAGCAG AGTGTAAAAG CATATCATAC TATGAATAAA
    TTTCTTGGCT CTTTCATCGA TCATGTTCAT AAGGAAATGG ACTACTTTAT AAAAGATAAA
    TTGCTTCTTG AAAGAATTCT TGGCATGGAA TTCATGGAAC CAATGGAAAA AAGCATCTTT
    TACAATGATG AAGGATATTC TTTCAGCAGT GTTCTGTATT ATGGAAATGA AGCTACTCTT
    CTTATTTATG ATCTGATGTT CTTCTGTGTT GTGGATTTGG CTTGCCAAAA TTTTATTTTA
    GCAGCCTTCC TTACATACCT ACAACAAGAG ATTTTTAGAT TTATTCGTAA TGCAGTAGGA
    CAGAAGAATT TGGCATCCAA AACACTGGTG GATCAAAGAT TCCTGATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192228.1
    CDS1..2991
    Translation

    Target ORF information:

    RefSeq Version NM_001205299.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transmembrane protein 67 (TMEM67), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACGG GAGGCCGCGG GGCTGTGGTT GCGATGGCCG CTGGGTCCAC CCTGTCGTCT 
    GCGAGTGTGA CTCTGTTCCT CTCGCTGGTC CTCCCTCGCG TCTCACAAGC TCAGACCTTC
    TTTTTCCCTT TCCGGCAGCC GGAGACGTGC GGCCACAACC AGTACTTCGA TATCTCGGCG
    CTCTCCTGTG TGGCGTGTGG TTCCAATCAG AGGCAGGATG CCGGAGGAAC TTCATGTGTG
    TGTCTACCAG GATTTCAGAT GATTTCTAAT AATGGAGGAC CTGATATTAC TTGTAAAAAG
    TGCCCAGAAC ACATGAAAGG TGTTACTGAA GATGGCTGGA ACTGTATTTC TTGCCCTGGT
    GGTTTAACTG CAGAAGGAAA ATGCCACTGT CCCACTGGCC ATATTTTAGT GGAAAGAAAT
    GTTAATGGAA CATTGTTGTC TCAAGCAACT TGTGAGCTCT GTGATGAAAG TGAAAACTCT
    TTTACAGTAG CAAATGCTTT AGGAAACAGG TGTGTCCGAT GTGAACCAGC GTTCATTAAT
    ACCAGCAAGT CCTGTGCATG TTCAGAACCT AACATTTTAA CAGGGGGATT GTGTTTGAGC
    AGCACAGGGA ATTTTCCTCT ACGTATGATT TCAACTGCAC GTTATGGAGA GCTTGGCATA
    TCTTTCACTT CAGAATGGTT TGCAAAGTAT TTGCAGTCAT CAGCAGCTGC TTGTTGGGTG
    TATGGCAATC TAACATCTTG CCAAGCTCTC GGAAATATGT GTGTAATGAA CATGAACTAT
    TATGACTCCT CTACTTTTGA TGCATGTGGA TTGTTTCAGT TTATCTTTGA AAATACTGCT
    GCACTGGGCA CTGTTCATTC TGTCTCCTTT TGGAGACAGA ATCTTCCATG GCTGTTCTAT
    GGAGACCAGC TAGGATTAGC ACCTCAAATA CTTAGTACTA CACCTCTTCC TACAAATTTC
    AGTTTTAAAG GGGAAAACCA GAGTACAAAA CTGAAGTTTG TTGCTGCTTC CTATGATGTG
    AGAGGAAATT TTCTCAAGTG GCAAACTTTA GAAGGAGGGA TTTTACAGCT TTGTCCAGAC
    ACAGAGACGA GACTAAATGC TGCTTATTCC TTTGGAACAA CTTATCAACA AAGTTGTGAG
    ATTCCTGTCC CTAAGATCTT AACTGACTTT CCCACTCCTA CGTTTTATGA TGTGTACCTT
    GAATATACTG ATGAGAATCA GCACCAATAT ATTTGGGCTG TGCCTGTGTT AAACTTAAAT
    CTTCAGCACA AGAAAATGTT TGTGAACAAA GACAGTAGTT CTGGCAAGTG GCTTCTGACT
    CGGCGCATTT TCTTAGTGGA TACACTAAGT GGACGGGAAA ATGACTTAGG AAGCCAGCCA
    AGATTAATTA GAGTCGCCAC CCAAATATCA CTGAGCATCC ACCTTGTACC CAACACAAAA
    AATGGAAACA TTTACCCTCC CTTAATTACC ATTGCCTACC ATGACATTGA TATCAAAGAT
    CCCAGCAGCC AGTCTGTGAA GGTTTCTTTC TCAGTCACAT ACGAAATGGA TCAAGGAGAA
    GCACATGTCC AGACAGATAT TGCTTTAGGT GTATTGGGTG GACTAGCTGT TTTATCATCT
    CTTTTGAAGA CAGCAGGTTG GAAGAGACGT ATTGGGAGTC CCATGATTGA TTTACAGACA
    GTTGCGAAAT TCTTGGTTTA CTATGCTGGT GATCTGGCCA ATGTTTTCTT TATCATCACT
    GTAGGAACAG GTCTTTACTG GCTTATTTTC TTTAAAGCAC AGAAGTCTGT CTCTGTTTTG
    CTGCCAGTGC CAGCTCAAGA AAAACGTTTT GTTACTTATG TGGGATGTGG TTTTGCTCTG
    AAGGCTCTAC AATTTTTGCA TAAGCTCATC TCCCAGATTA CCATAGACAT ATTCTTTATC
    GATTGGGAGC GGCCTAAAGG AAAGGTTCTT AAAGCTGTTG AAGGTGAGGG TGGTGTTCGG
    AGTGCTACTG TTCCTGTAAG CATATGGAGA ACTTATTTTG TGGCAAATGA ATGGAACGAA
    ATTCAGACTG TGAGAAAAAT TAACCCACTC TTTCAAGTAC TTACTGTCCT CTTCCTTTTG
    GAGGTTGTGG GATTTAAGAA CTTAGCATTA ATGGACTCAT CCTCTAGTCT TTCCAGAAGC
    CCACCTAGCT ACATAGCTCC TTACAGCCGC ATTTTGAGAT ACGCGGTATC TTCTGCTCTT
    TGGCTGGTCA TTGGACTTAT ACAGATTGTG TTCTTTGCCG TCTTCTATGA GAGATTTATA
    GAAGATAAAA TTCGACAGTT TGTTGATTTG TGCTGTATGA GTAATATATC AGTGTTTCTG
    TTATCCCACA GATGTTTTGG CTACTACATC CATGGTAGAT CAGTACATGG GCATGCTGAT
    ACTAATATGG AAGAAATGAA TATGAATCTT AAAAGAGAAG CGGAAAATTT ATGTAGCCAA
    AGAGGTTTGG TACCTAATAC AGATGGTCAG ACTTTTCAGA TTGCAATTTC TAGCCAGATG
    AGGCAACACT ATGACAGAAT TCATGAGACA CTAACAAGGA AAAATGGCCC TGCTAGACTA
    TTGAGTTCCT CAGGAAGTAC TTTCGAGCAG AGTGTAAAAG CATATCATAC TATGAATAAA
    TTTCTTGGCT CTTTCATCGA TCATGTTCAT AAGGAAATGG ACTACTTTAT AAAAGATAAA
    TTGCTTCTTG AAAGAATTCT TGGCATGGAA TTCATGGAAC CAATGGAAAA AAGCATCTTT
    TACAATGATG AAGGATATTC TTTCAGCAGT GTTCTGTATT ATGGAAATGA AGCTACTCTT
    CTTATTTATG ATCTGATGTT CTTCTGTGTT GTGGATTTGG CTTGCCAAAA TTTTATTTTA
    GCAGCCTTCC TTACATACCT ACAACAAGAG ATTTTTAGAT TTATTCGTAA TGCAGTAGGA
    CAGAAGAATT TGGCATCCAA AACACTGGTG GATCAAAGAT TCCTGATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222221
    Clone ID Related Accession (Same CDS sequence) XM_025001457.1
    Accession Version XM_025001457.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product meckelin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACGG GAGGCCGCGG GGCTGTGGTT GCGATGGCCG CTGGGTCCAC CCTGTCGTCT 
    GCGAGTGTGA CTCTGTTCCT CTCGCTGGTC CTCCCTCGCG TCTCACAAGC TCAGACCTTC
    TTTTTCCCTT TCCGGCAGCC GGAGACGTGC GGCCACAACC AGTACTTCGA TATCTCGGCG
    CTCTCCTGTG TGGCGTGTGG TTCCAATCAG AGGCAGGATG CCGGAGGAAC TTCATGTGTG
    TGTCTACCAG GATTTCAGAT GATTTCTAAT AATGGAGGAC CTGATATTAC TTGTAAAAAG
    TGCCCAGAAC ACATGAAAGG TGTTACTGAA GATGGCTGGA ACTGTATTTC TTGCCCTGGT
    GGTTTAACTG CAGAAGGAAA ATGCCACTGT CCCACTGGCC ATATTTTAGT GGAAAGAAAT
    GTTAATGGAA CATTGTTGTC TCAAGCAACT TGTGAGCTCT GTGATGAAAG TGAAAACTCT
    TTTACAGTAG CAAATGCTTT AGGAAACAGG TGTGTCCGAT GTGAACCAGC GTTCATTAAT
    ACCAGCAAGT CCTGTGCATG TTCAGAACCT AACATTTTAA CAGGGGGATT GTGTTTGAGC
    AGCACAGGGA ATTTTCCTCT ACGTATGATT TCAACTGCAC GTTATGGAGA GCTTGGCATA
    TCTTTCACTT CAGAATGGTT TGCAAAGTAT TTGCAGTCAT CAGCAGCTGC TTGTTGGGTG
    TATGGCAATC TAACATCTTG CCAAGCTCTC GGAAATATGT GTGTAATGAA CATGAACTAT
    TATGACTCCT CTACTTTTGA TGCATGTGGA TTGTTTCAGT TTATCTTTGA AAATACTGCT
    GCACTGGGCA CTGTTCATTC TGTCTCCTTT TGGAGACAGA ATCTTCCATG GCTGTTCTAT
    GGAGACCAGC TAGGATTAGC ACCTCAAATA CTTAGTACTA CACCTCTTCC TACAAATTTC
    AGTTTTAAAG GGGAAAACCA GCAGAGTACA AAACTGAAGT TTGTTGCTGC TTCCTATGAT
    GTGAGAGGAA ATTTTCTCAA GTGGCAAACT TTAGAAGGAG GGATTTTACA GCTTTGTCCA
    GACACAGAGA CGAGACTAAA TGCTGCTTAT TCCTTTGGAA CAACTTATCA ACAAAGTTGT
    GAGATTCCTG TCCCTAAGAT CTTAACTGAC TTTCCCACTC CTACGTTTTA TGATGTGTAC
    CTTGAATATA CTGATGAGAA TCAGCACCAA TATATTTGGG CTGTGCCTGT GTTAAACTTA
    AATCTTCAGC ACAAGAAAAT GTTTGTGAAC AAAGACAGTA GTTCTGGCAA GTGGCTTCTG
    ACTCGGCGCA TTTTCTTAGT GGATACACTA AGTGGACGGG AAAATGACTT AGGAAGCCAG
    CCAAGATTAA TTAGAGTCGC CACCCAAATA TCACTGAGCA TCCACCTTGT ACCCAACACA
    AAAAATGGAA ACATTTACCC TCCCTTAATT ACCATTGCCT ACCATGACAT TGATATCAAA
    GATCCCAGCA GCCAGTCTGT GAAGGTTTCT TTCTCAGTCA CATACGAAAT GGATCAAGGA
    GAAGCACATG TCCAGACAGA TATTGCTTTA GGTGTATTGG GTGGACTAGC TGTTTTATCA
    TCTCTTTTGA AGACAGCAGG TTGGAAGAGA CGTATTGGGA GTCCCATGAT TGATTTACAG
    ACAGTTGCGA AATTCTTGGT TTACTATGCT GGTGATCTGG CCAATGTTTT CTTTATCATC
    ACTGTAGGAA CAGGTCTTTA CTGGCTTATT TTCTTTAAAG CACAGAAGTC TGTCTCTGTT
    TTGCTGCCAG TGCCAGCTCA AGAAAAACGT TTTGTTACTT ATGTGGGATG TGGTTTTGCT
    CTGAAGGCTC TACAATTTTT GCATAAGCTC ATCTCCCAGA TTACCATAGA CATATTCTTT
    ATCGATTGGG AGCGGCCTAA AGGAAAGGTT CTTAAAGCTG TTGAAGGTGA GGGTGGTGTT
    CGGAGTGCTA CTGTTCCTGT AAGCATATGG AGAACTTATT TTGTGGCAAA TGAATGGAAC
    GAAATTCAGA CTGTGAGAAA AATTAACCCA CTCTTTCAAG TACTTACTGT CCTCTTCCTT
    TTGGAGGTTG TGGGATTTAA GAACTTAGCA TTAATGGACT CATCCTCTAG TCTTTCCAGA
    AGCCCACCTA GCTACATAGC TCCTTACAGC CGCATTTTGA GATACGCGGT ATCTTCTGCT
    CTTTGGCTGG TCATTGGACT TATACAGATT GTGTTCTTTG CCGTCTTCTA TGAGAGATTT
    ATAGAAGATA AAATTCGACA GTTTGTTGAT TTGTGCTGTA TGAGTAATAT ATCAGTGTTT
    CTGTTATCCC ACAGATGTTT TGGCTACTAC ATCCATGGTA GATCAGTACA TGGGCATGCT
    GATACTAATA TGGAAGAAAT GAATATGAAT CTTAAAAGAG AAGCGGAAAA TTTATGTAGC
    CAAAGAGGTT TGGTACCTAA TACAGATGGT CAGACTTTTC AGATTGCAAT TTCTAGCCAG
    ATGAGGCAAC ACTATGACAG AATTCATGAG ACACTAACAA GGAAAAATGG CCCTGCTAGA
    CTATTGAGTT CCTCAGGAAG TACTTTCGAG CAGAGTGTAA AAGCATATCA TACTATGAAT
    AAATTTCTTG GCTCTTTCAT CGATCATGTT CATAAGGAAA TGGACTACTT TATAAAAGAT
    AAATTGCTTC TTGAAAGAAT TCTTGGCATG GAATTCATGG AACCAATGGA AAAAAGCATC
    TTTTACAATG ATGAAGGATA TTCTTTCAGC AGTGTTCTGT ATTATGGAAA TGAAGCTACT
    CTTCTTATTT ATGATCTGAT GTTCTTCTGT GTTGTGGATT TGGCTTGCCA AAATTTTATT
    TTAGCAGCCT TCCTTACATA CCTACAACAA GAGATTTTTA GATTTATTCG TAATGCAGTA
    GGACAGAAGA ATTTGGCATC CAAAACACTG GTGGATCAAA GATTCCTGAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857225.1
    CDS58..3051
    Translation

    Target ORF information:

    RefSeq Version XM_025001457.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transmembrane protein 67 (TMEM67), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACGG GAGGCCGCGG GGCTGTGGTT GCGATGGCCG CTGGGTCCAC CCTGTCGTCT 
    GCGAGTGTGA CTCTGTTCCT CTCGCTGGTC CTCCCTCGCG TCTCACAAGC TCAGACCTTC
    TTTTTCCCTT TCCGGCAGCC GGAGACGTGC GGCCACAACC AGTACTTCGA TATCTCGGCG
    CTCTCCTGTG TGGCGTGTGG TTCCAATCAG AGGCAGGATG CCGGAGGAAC TTCATGTGTG
    TGTCTACCAG GATTTCAGAT GATTTCTAAT AATGGAGGAC CTGATATTAC TTGTAAAAAG
    TGCCCAGAAC ACATGAAAGG TGTTACTGAA GATGGCTGGA ACTGTATTTC TTGCCCTGGT
    GGTTTAACTG CAGAAGGAAA ATGCCACTGT CCCACTGGCC ATATTTTAGT GGAAAGAAAT
    GTTAATGGAA CATTGTTGTC TCAAGCAACT TGTGAGCTCT GTGATGAAAG TGAAAACTCT
    TTTACAGTAG CAAATGCTTT AGGAAACAGG TGTGTCCGAT GTGAACCAGC GTTCATTAAT
    ACCAGCAAGT CCTGTGCATG TTCAGAACCT AACATTTTAA CAGGGGGATT GTGTTTGAGC
    AGCACAGGGA ATTTTCCTCT ACGTATGATT TCAACTGCAC GTTATGGAGA GCTTGGCATA
    TCTTTCACTT CAGAATGGTT TGCAAAGTAT TTGCAGTCAT CAGCAGCTGC TTGTTGGGTG
    TATGGCAATC TAACATCTTG CCAAGCTCTC GGAAATATGT GTGTAATGAA CATGAACTAT
    TATGACTCCT CTACTTTTGA TGCATGTGGA TTGTTTCAGT TTATCTTTGA AAATACTGCT
    GCACTGGGCA CTGTTCATTC TGTCTCCTTT TGGAGACAGA ATCTTCCATG GCTGTTCTAT
    GGAGACCAGC TAGGATTAGC ACCTCAAATA CTTAGTACTA CACCTCTTCC TACAAATTTC
    AGTTTTAAAG GGGAAAACCA GCAGAGTACA AAACTGAAGT TTGTTGCTGC TTCCTATGAT
    GTGAGAGGAA ATTTTCTCAA GTGGCAAACT TTAGAAGGAG GGATTTTACA GCTTTGTCCA
    GACACAGAGA CGAGACTAAA TGCTGCTTAT TCCTTTGGAA CAACTTATCA ACAAAGTTGT
    GAGATTCCTG TCCCTAAGAT CTTAACTGAC TTTCCCACTC CTACGTTTTA TGATGTGTAC
    CTTGAATATA CTGATGAGAA TCAGCACCAA TATATTTGGG CTGTGCCTGT GTTAAACTTA
    AATCTTCAGC ACAAGAAAAT GTTTGTGAAC AAAGACAGTA GTTCTGGCAA GTGGCTTCTG
    ACTCGGCGCA TTTTCTTAGT GGATACACTA AGTGGACGGG AAAATGACTT AGGAAGCCAG
    CCAAGATTAA TTAGAGTCGC CACCCAAATA TCACTGAGCA TCCACCTTGT ACCCAACACA
    AAAAATGGAA ACATTTACCC TCCCTTAATT ACCATTGCCT ACCATGACAT TGATATCAAA
    GATCCCAGCA GCCAGTCTGT GAAGGTTTCT TTCTCAGTCA CATACGAAAT GGATCAAGGA
    GAAGCACATG TCCAGACAGA TATTGCTTTA GGTGTATTGG GTGGACTAGC TGTTTTATCA
    TCTCTTTTGA AGACAGCAGG TTGGAAGAGA CGTATTGGGA GTCCCATGAT TGATTTACAG
    ACAGTTGCGA AATTCTTGGT TTACTATGCT GGTGATCTGG CCAATGTTTT CTTTATCATC
    ACTGTAGGAA CAGGTCTTTA CTGGCTTATT TTCTTTAAAG CACAGAAGTC TGTCTCTGTT
    TTGCTGCCAG TGCCAGCTCA AGAAAAACGT TTTGTTACTT ATGTGGGATG TGGTTTTGCT
    CTGAAGGCTC TACAATTTTT GCATAAGCTC ATCTCCCAGA TTACCATAGA CATATTCTTT
    ATCGATTGGG AGCGGCCTAA AGGAAAGGTT CTTAAAGCTG TTGAAGGTGA GGGTGGTGTT
    CGGAGTGCTA CTGTTCCTGT AAGCATATGG AGAACTTATT TTGTGGCAAA TGAATGGAAC
    GAAATTCAGA CTGTGAGAAA AATTAACCCA CTCTTTCAAG TACTTACTGT CCTCTTCCTT
    TTGGAGGTTG TGGGATTTAA GAACTTAGCA TTAATGGACT CATCCTCTAG TCTTTCCAGA
    AGCCCACCTA GCTACATAGC TCCTTACAGC CGCATTTTGA GATACGCGGT ATCTTCTGCT
    CTTTGGCTGG TCATTGGACT TATACAGATT GTGTTCTTTG CCGTCTTCTA TGAGAGATTT
    ATAGAAGATA AAATTCGACA GTTTGTTGAT TTGTGCTGTA TGAGTAATAT ATCAGTGTTT
    CTGTTATCCC ACAGATGTTT TGGCTACTAC ATCCATGGTA GATCAGTACA TGGGCATGCT
    GATACTAATA TGGAAGAAAT GAATATGAAT CTTAAAAGAG AAGCGGAAAA TTTATGTAGC
    CAAAGAGGTT TGGTACCTAA TACAGATGGT CAGACTTTTC AGATTGCAAT TTCTAGCCAG
    ATGAGGCAAC ACTATGACAG AATTCATGAG ACACTAACAA GGAAAAATGG CCCTGCTAGA
    CTATTGAGTT CCTCAGGAAG TACTTTCGAG CAGAGTGTAA AAGCATATCA TACTATGAAT
    AAATTTCTTG GCTCTTTCAT CGATCATGTT CATAAGGAAA TGGACTACTT TATAAAAGAT
    AAATTGCTTC TTGAAAGAAT TCTTGGCATG GAATTCATGG AACCAATGGA AAAAAGCATC
    TTTTACAATG ATGAAGGATA TTCTTTCAGC AGTGTTCTGT ATTATGGAAA TGAAGCTACT
    CTTCTTATTT ATGATCTGAT GTTCTTCTGT GTTGTGGATT TGGCTTGCCA AAATTTTATT
    TTAGCAGCCT TCCTTACATA CCTACAACAA GAGATTTTTA GATTTATTCG TAATGCAGTA
    GGACAGAAGA ATTTGGCATC CAAAACACTG GTGGATCAAA GATTCCTGAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222222
    Clone ID Related Accession (Same CDS sequence) XM_025001458.1
    Accession Version XM_025001458.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product meckelin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACGG GAGGCCGCGG GGCTGTGGTT GCGATGGCCG CTGGGTCCAC CCTGTCGTCT 
    GCGAGTGTGA CTCTGTTCCT CTCGCTGGTC CTCCCTCGCG TCTCACAAGC TCAGACCTTC
    TTTTTCCCTT TCCGGCAGCC GGAGACGTGC GGCCACAACC AGTACTTCGA TATCTCGGCG
    CTCTCCTGTG TGGCGTGTGG TTCCAATCAG AGGCAGGATG CCGGAGGAAC TTCATGTGTG
    TGTCTACCAG GATTTCAGAT GATTTCTAAT AATGGAGGAC CTGATATTAC TTGTAAAAAG
    TGCCCAGAAC ACATGAAAGG TGTTACTGAA GATGGCTGGA ACTGTATTTC TTGCCCTGGT
    GGTTTAACTG CAGAAGGAAA ATGCCACTGT CCCACTGGCC ATATTTTAGT GGAAAGAAAT
    GTTAATGGAA CATTGTTGTC TCAAGCAACT TGTGAGCTCT GTGATGAAAG TGAAAACTCT
    TTTACAGTAG CAAATGCTTT AGGAAACAGG TGTGTCCGAT GTGAACCAGC GTTCATTAAT
    ACCAGCAAGT CCTGTGCATG TTCAGAACCT AACATTTTAA CAGGGGGATT GTGTTTGAGC
    AGCACAGGGA ATTTTCCTCT ACGTATGATT TCAACTGCAC GTTATGGAGA GCTTGGCATA
    TCTTTCACTT CAGAATGGTT TGCAAAGTAT TTGCAGTCAT CAGCAGCTGC TTGTTGGGTG
    TATGGCAATC TAACATCTTG CCAAGCTCTC GGAAATATGT GTGTAATGAA CATGAACTAT
    TATGACTCCT CTACTTTTGA TGCATGTGGA TTGTTTCAGT TTATCTTTGA AAATACTGCT
    GCACTGGGCA CTGTTCATTC TGTCTCCTTT TGGAGACAGA ATCTTCCATG GCTGTTCTAT
    GGAGACCAGC TAGGATTAGC ACCTCAAATA CTTAGTACTA CACCTCTTCC TACAAATTTC
    AGTTTTAAAG GGGAAAACCA GCAGAGTACA AAACTGAAGT TTGTTGCTGC TTCCTATGAT
    GTGAGAGGAA ATTTTCTCAA GTGGCAAACT TTAGAAGGAG GGATTTTACA GCTTTGTCCA
    GACACAGAGA CGAGACTAAA TGCTGCTTAT TCCTTTGGAA CAACTTATCA ACAAAGTTGT
    GAGATTCCTG TCCCTAAGAT CTTAACTGAC TTTCCCACTC CTACGTTTTA TGATGTGTAC
    CTTGAATATA CTGATGAGAA TCAGCACCAA TATATTTGGG CTGTGCCTGT GTTAAACTTA
    AATCTTCAGC ACAAGAAAAT GTTTGTGAAC AAAGACAGTA GTTCTGGCAA GTGGCTTCTG
    ACTCGGCGCA TTTTCTTAGT GGATACACTA AGTGGACGGG AAAATGACTT AGGAAGCCAG
    CCAAGATTAA TTAGAGTCGC CACCCAAATA TCACTGAGCA TCCACCTTGT ACCCAACACA
    AAAAATGGAA ACATTTACCC TCCCTTAATT ACCATTGCCT ACCATGACAT TGATATCAAA
    GATCCCAGCA GCCAGTCTGT GAAGGTTTCT TTCTCAGTCA CATACGAAAT GGATCAAGGA
    GAAGCACATG TCCAGACAGA TATTGCTTTA GGTGTATTGG GTGGACTAGC TGTTTTATCA
    TCTCTTTTGA AGACAGCAGG TTGGAAGAGA CGTATTGGGA GTCCCATGAT TGATTTACAG
    ACAGTTGCGA AATTCTTGGT TTACTATGCT GGTGATCTGG CCAATGTTTT CTTTATCATC
    ACTGTAGGAA CAGGTCTTTA CTGGCTTATT TTCTTTAAAG CTCTACAATT TTTGCATAAG
    CTCATCTCCC AGATTACCAT AGACATATTC TTTATCGATT GGGAGCGGCC TAAAGGAAAG
    GTTCTTAAAG CTGTTGAAGG TGAGGGTGGT GTTCGGAGTG CTACTGTTCC TGTAAGCATA
    TGGAGAACTT ATTTTGTGGC AAATGAATGG AACGAAATTC AGACTGTGAG AAAAATTAAC
    CCACTCTTTC AAGTACTTAC TGTCCTCTTC CTTTTGGAGG TTGTGGGATT TAAGAACTTA
    GCATTAATGG ACTCATCCTC TAGTCTTTCC AGAAGCCCAC CTAGCTACAT AGCTCCTTAC
    AGCCGCATTT TGAGATACGC GGTATCTTCT GCTCTTTGGC TGGTCATTGG ACTTATACAG
    ATTGTGTTCT TTGCCGTCTT CTATGAGAGA TTTATAGAAG ATAAAATTCG ACAGTTTGTT
    GATTTGTGCT GTATGAGTAA TATATCAGTG TTTCTGTTAT CCCACAGATG TTTTGGCTAC
    TACATCCATG GTAGATCAGT ACATGGGCAT GCTGATACTA ATATGGAAGA AATGAATATG
    AATCTTAAAA GAGAAGCGGA AAATTTATGT AGCCAAAGAG GTTTGGTACC TAATACAGAT
    GGTCAGACTT TTCAGATTGC AATTTCTAGC CAGATGAGGC AACACTATGA CAGAATTCAT
    GAGACACTAA CAAGGAAAAA TGGCCCTGCT AGACTATTGA GTTCCTCAGG AAGTACTTTC
    GAGCAGAGTG TAAAAGCATA TCATACTATG AATAAATTTC TTGGCTCTTT CATCGATCAT
    GTTCATAAGG AAATGGACTA CTTTATAAAA GATAAATTGC TTCTTGAAAG AATTCTTGGC
    ATGGAATTCA TGGAACCAAT GGAAAAAAGC ATCTTTTACA ATGATGAAGG ATATTCTTTC
    AGCAGTGTTC TGTATTATGG AAATGAAGCT ACTCTTCTTA TTTATGATCT GATGTTCTTC
    TGTGTTGTGG ATTTGGCTTG CCAAAATTTT ATTTTAGCAG CCTTCCTTAC ATACCTACAA
    CAAGAGATTT TTAGATTTAT TCGTAATGCA GTAGGACAGA AGAATTTGGC ATCCAAAACA
    CTGGTGGATC AAAGATTCCT GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857226.1
    CDS58..2964
    Translation

    Target ORF information:

    RefSeq Version XM_025001458.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transmembrane protein 67 (TMEM67), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACGG GAGGCCGCGG GGCTGTGGTT GCGATGGCCG CTGGGTCCAC CCTGTCGTCT 
    GCGAGTGTGA CTCTGTTCCT CTCGCTGGTC CTCCCTCGCG TCTCACAAGC TCAGACCTTC
    TTTTTCCCTT TCCGGCAGCC GGAGACGTGC GGCCACAACC AGTACTTCGA TATCTCGGCG
    CTCTCCTGTG TGGCGTGTGG TTCCAATCAG AGGCAGGATG CCGGAGGAAC TTCATGTGTG
    TGTCTACCAG GATTTCAGAT GATTTCTAAT AATGGAGGAC CTGATATTAC TTGTAAAAAG
    TGCCCAGAAC ACATGAAAGG TGTTACTGAA GATGGCTGGA ACTGTATTTC TTGCCCTGGT
    GGTTTAACTG CAGAAGGAAA ATGCCACTGT CCCACTGGCC ATATTTTAGT GGAAAGAAAT
    GTTAATGGAA CATTGTTGTC TCAAGCAACT TGTGAGCTCT GTGATGAAAG TGAAAACTCT
    TTTACAGTAG CAAATGCTTT AGGAAACAGG TGTGTCCGAT GTGAACCAGC GTTCATTAAT
    ACCAGCAAGT CCTGTGCATG TTCAGAACCT AACATTTTAA CAGGGGGATT GTGTTTGAGC
    AGCACAGGGA ATTTTCCTCT ACGTATGATT TCAACTGCAC GTTATGGAGA GCTTGGCATA
    TCTTTCACTT CAGAATGGTT TGCAAAGTAT TTGCAGTCAT CAGCAGCTGC TTGTTGGGTG
    TATGGCAATC TAACATCTTG CCAAGCTCTC GGAAATATGT GTGTAATGAA CATGAACTAT
    TATGACTCCT CTACTTTTGA TGCATGTGGA TTGTTTCAGT TTATCTTTGA AAATACTGCT
    GCACTGGGCA CTGTTCATTC TGTCTCCTTT TGGAGACAGA ATCTTCCATG GCTGTTCTAT
    GGAGACCAGC TAGGATTAGC ACCTCAAATA CTTAGTACTA CACCTCTTCC TACAAATTTC
    AGTTTTAAAG GGGAAAACCA GCAGAGTACA AAACTGAAGT TTGTTGCTGC TTCCTATGAT
    GTGAGAGGAA ATTTTCTCAA GTGGCAAACT TTAGAAGGAG GGATTTTACA GCTTTGTCCA
    GACACAGAGA CGAGACTAAA TGCTGCTTAT TCCTTTGGAA CAACTTATCA ACAAAGTTGT
    GAGATTCCTG TCCCTAAGAT CTTAACTGAC TTTCCCACTC CTACGTTTTA TGATGTGTAC
    CTTGAATATA CTGATGAGAA TCAGCACCAA TATATTTGGG CTGTGCCTGT GTTAAACTTA
    AATCTTCAGC ACAAGAAAAT GTTTGTGAAC AAAGACAGTA GTTCTGGCAA GTGGCTTCTG
    ACTCGGCGCA TTTTCTTAGT GGATACACTA AGTGGACGGG AAAATGACTT AGGAAGCCAG
    CCAAGATTAA TTAGAGTCGC CACCCAAATA TCACTGAGCA TCCACCTTGT ACCCAACACA
    AAAAATGGAA ACATTTACCC TCCCTTAATT ACCATTGCCT ACCATGACAT TGATATCAAA
    GATCCCAGCA GCCAGTCTGT GAAGGTTTCT TTCTCAGTCA CATACGAAAT GGATCAAGGA
    GAAGCACATG TCCAGACAGA TATTGCTTTA GGTGTATTGG GTGGACTAGC TGTTTTATCA
    TCTCTTTTGA AGACAGCAGG TTGGAAGAGA CGTATTGGGA GTCCCATGAT TGATTTACAG
    ACAGTTGCGA AATTCTTGGT TTACTATGCT GGTGATCTGG CCAATGTTTT CTTTATCATC
    ACTGTAGGAA CAGGTCTTTA CTGGCTTATT TTCTTTAAAG CTCTACAATT TTTGCATAAG
    CTCATCTCCC AGATTACCAT AGACATATTC TTTATCGATT GGGAGCGGCC TAAAGGAAAG
    GTTCTTAAAG CTGTTGAAGG TGAGGGTGGT GTTCGGAGTG CTACTGTTCC TGTAAGCATA
    TGGAGAACTT ATTTTGTGGC AAATGAATGG AACGAAATTC AGACTGTGAG AAAAATTAAC
    CCACTCTTTC AAGTACTTAC TGTCCTCTTC CTTTTGGAGG TTGTGGGATT TAAGAACTTA
    GCATTAATGG ACTCATCCTC TAGTCTTTCC AGAAGCCCAC CTAGCTACAT AGCTCCTTAC
    AGCCGCATTT TGAGATACGC GGTATCTTCT GCTCTTTGGC TGGTCATTGG ACTTATACAG
    ATTGTGTTCT TTGCCGTCTT CTATGAGAGA TTTATAGAAG ATAAAATTCG ACAGTTTGTT
    GATTTGTGCT GTATGAGTAA TATATCAGTG TTTCTGTTAT CCCACAGATG TTTTGGCTAC
    TACATCCATG GTAGATCAGT ACATGGGCAT GCTGATACTA ATATGGAAGA AATGAATATG
    AATCTTAAAA GAGAAGCGGA AAATTTATGT AGCCAAAGAG GTTTGGTACC TAATACAGAT
    GGTCAGACTT TTCAGATTGC AATTTCTAGC CAGATGAGGC AACACTATGA CAGAATTCAT
    GAGACACTAA CAAGGAAAAA TGGCCCTGCT AGACTATTGA GTTCCTCAGG AAGTACTTTC
    GAGCAGAGTG TAAAAGCATA TCATACTATG AATAAATTTC TTGGCTCTTT CATCGATCAT
    GTTCATAAGG AAATGGACTA CTTTATAAAA GATAAATTGC TTCTTGAAAG AATTCTTGGC
    ATGGAATTCA TGGAACCAAT GGAAAAAAGC ATCTTTTACA ATGATGAAGG ATATTCTTTC
    AGCAGTGTTC TGTATTATGG AAATGAAGCT ACTCTTCTTA TTTATGATCT GATGTTCTTC
    TGTGTTGTGG ATTTGGCTTG CCAAAATTTT ATTTTAGCAG CCTTCCTTAC ATACCTACAA
    CAAGAGATTT TTAGATTTAT TCGTAATGCA GTAGGACAGA AGAATTTGGC ATCCAAAACA
    CTGGTGGATC AAAGATTCCT GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222223
    Clone ID Related Accession (Same CDS sequence) XM_025001459.1
    Accession Version XM_025001459.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2808bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product meckelin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACGG GAGGCCGCGG GGCTGTGGTT GCGATGGCCG CTGGGTCCAC CCTGTCGTCT 
    GCGAGTGTGA CTCTGTTCCT CTCGCTGGTC CTCCCTCGCG TCTCACAAGC TCAGACCTTC
    TTTTTCCCTT TCCGGCAGCC GGAGACGTGC GGCCACAACC AGTACTTCGA TATCTCGGCG
    CTCTCCTGTG TGGCGTGTGG TTCCAATCAG AGGCAGGATG CCGGAGGAAC TTCATGTGTG
    TGTCTACCAG GATTTCAGAT GATTTCTAAT AATGGAGGAC CTGATATTAC TTGTAAAAAG
    TGCCCAGAAC ACATGAAAGG TGTTACTGAA GATGGCTGGA ACTGTATTTC TTGCCCTGGT
    GGTTTAACTG CAGAAGGAAA ATGCCACTGT CCCACTGGCC ATATTTTAGT GGAAAGAAAT
    GTTAATGGAA CATTGTTGTC TCAAGCAACT TGTGAGCTCT GTGATGAAAG TGAAAACTCT
    TTTACAGTAG CAAATGCTTT AGGAAACAGG TGTGTCCGAT GTGAACCAGC GTTCATTAAT
    ACCAGCAAGT CCTGTGCATG TTCAGAACCT AACATTTTAA CAGGGGGATT GTGTTTGAGC
    AGCACAGGGA ATTTTCCTCT ACGTATGATT TCAACTGCAC GTTATGGAGA GCTTGGCATA
    TCTTTCACTT CAGAATGGTT TGCAAAGTAT TTGCAGTCAT CAGCAGCTGC TTGTTGGGTG
    TATGGCAATC TAACATCTTG CCAAGCTCTC GGAAATATGT GTGTAATGAA CATGAACTAT
    TATGACTCCT CTACTTTTGA TGCATGTGGA TTGTTTCAGT TTATCTTTGA AAATACTGCT
    GCACTGGGCA CTGTTCATTC TGTCTCCTTT TGGAGACAGA ATCTTCCATG GCTGTTCTAT
    GGAGACCAGC TAGGATTAGC ACCTCAAATA CTTAGTACTA CACCTCTTCC TACAAATTTC
    AGTTTTAAAG GGGAAAACCA GCAGAGTACA AAACTGAAGT TTGTTGCTGC TTCCTATGAT
    GTGAGAGGAA ATTTTCTCAA GTGGCAAACT TTAGAAGGAG GGATTTTACA GCTTTGTCCA
    GACACAGAGA CGAGACTAAA TGCTGCTTAT TCCTTTGGAA CAACTTATCA ACAAAGTTGT
    GAGATTCCTG TCCCTAAGAT CTTAACTGAC TTTCCCACTC CTACGTTTTA TGATGTGTAC
    CTTGAATATA CTGATGAGAA TCAGCACCAA TATATTTGGG CTGTGCCTGT GTTAAACTTA
    AATCTTCAGC ACAAGAAAAT GTTTGTGAAC AAAGACAGTA GTTCTGGCAA GTGGCTTCTG
    ACTCGGCGCA TTTTCTTAGT GGATACACTA AGTGGACGGG AAAATGACTT AGGAAGCCAG
    CCAAGATTAA TTAGAGTCGC CACCCAAATA TCACTGAGCA TCCACCTTGT ACCCAACACA
    AAAAATGGAA ACATTTACCC TCCCTTAATT ACCATTGCCT ACCATGACAT TGATATCAAA
    GATCCCAGCA GCCAGTCTGT GAAGGTTTCT TTCTCAGTCA CATACGAAAT GGATCAAGGA
    GAAGCACATG TCCAGACAGA TATTGCTTTA GGTGTATTGG GTGGACTAGC TGTTTTATCA
    TCTCTTTTGA AGACAGCAGG TTGGAAGAGA CGTATTGGGA GTCCCATGAT TGATTTACAG
    GCTCTACAAT TTTTGCATAA GCTCATCTCC CAGATTACCA TAGACATATT CTTTATCGAT
    TGGGAGCGGC CTAAAGGAAA GGTTCTTAAA GCTGTTGAAG GTGAGGGTGG TGTTCGGAGT
    GCTACTGTTC CTGTAAGCAT ATGGAGAACT TATTTTGTGG CAAATGAATG GAACGAAATT
    CAGACTGTGA GAAAAATTAA CCCACTCTTT CAAGTACTTA CTGTCCTCTT CCTTTTGGAG
    GTTGTGGGAT TTAAGAACTT AGCATTAATG GACTCATCCT CTAGTCTTTC CAGAAGCCCA
    CCTAGCTACA TAGCTCCTTA CAGCCGCATT TTGAGATACG CGGTATCTTC TGCTCTTTGG
    CTGGTCATTG GACTTATACA GATTGTGTTC TTTGCCGTCT TCTATGAGAG ATTTATAGAA
    GATAAAATTC GACAGTTTGT TGATTTGTGC TGTATGAGTA ATATATCAGT GTTTCTGTTA
    TCCCACAGAT GTTTTGGCTA CTACATCCAT GGTAGATCAG TACATGGGCA TGCTGATACT
    AATATGGAAG AAATGAATAT GAATCTTAAA AGAGAAGCGG AAAATTTATG TAGCCAAAGA
    GGTTTGGTAC CTAATACAGA TGGTCAGACT TTTCAGATTG CAATTTCTAG CCAGATGAGG
    CAACACTATG ACAGAATTCA TGAGACACTA ACAAGGAAAA ATGGCCCTGC TAGACTATTG
    AGTTCCTCAG GAAGTACTTT CGAGCAGAGT GTAAAAGCAT ATCATACTAT GAATAAATTT
    CTTGGCTCTT TCATCGATCA TGTTCATAAG GAAATGGACT ACTTTATAAA AGATAAATTG
    CTTCTTGAAA GAATTCTTGG CATGGAATTC ATGGAACCAA TGGAAAAAAG CATCTTTTAC
    AATGATGAAG GATATTCTTT CAGCAGTGTT CTGTATTATG GAAATGAAGC TACTCTTCTT
    ATTTATGATC TGATGTTCTT CTGTGTTGTG GATTTGGCTT GCCAAAATTT TATTTTAGCA
    GCCTTCCTTA CATACCTACA ACAAGAGATT TTTAGATTTA TTCGTAATGC AGTAGGACAG
    AAGAATTTGG CATCCAAAAC ACTGGTGGAT CAAAGATTCC TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857227.1
    CDS58..2865
    Translation

    Target ORF information:

    RefSeq Version XM_025001459.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transmembrane protein 67 (TMEM67), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001459.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACGG GAGGCCGCGG GGCTGTGGTT GCGATGGCCG CTGGGTCCAC CCTGTCGTCT 
    GCGAGTGTGA CTCTGTTCCT CTCGCTGGTC CTCCCTCGCG TCTCACAAGC TCAGACCTTC
    TTTTTCCCTT TCCGGCAGCC GGAGACGTGC GGCCACAACC AGTACTTCGA TATCTCGGCG
    CTCTCCTGTG TGGCGTGTGG TTCCAATCAG AGGCAGGATG CCGGAGGAAC TTCATGTGTG
    TGTCTACCAG GATTTCAGAT GATTTCTAAT AATGGAGGAC CTGATATTAC TTGTAAAAAG
    TGCCCAGAAC ACATGAAAGG TGTTACTGAA GATGGCTGGA ACTGTATTTC TTGCCCTGGT
    GGTTTAACTG CAGAAGGAAA ATGCCACTGT CCCACTGGCC ATATTTTAGT GGAAAGAAAT
    GTTAATGGAA CATTGTTGTC TCAAGCAACT TGTGAGCTCT GTGATGAAAG TGAAAACTCT
    TTTACAGTAG CAAATGCTTT AGGAAACAGG TGTGTCCGAT GTGAACCAGC GTTCATTAAT
    ACCAGCAAGT CCTGTGCATG TTCAGAACCT AACATTTTAA CAGGGGGATT GTGTTTGAGC
    AGCACAGGGA ATTTTCCTCT ACGTATGATT TCAACTGCAC GTTATGGAGA GCTTGGCATA
    TCTTTCACTT CAGAATGGTT TGCAAAGTAT TTGCAGTCAT CAGCAGCTGC TTGTTGGGTG
    TATGGCAATC TAACATCTTG CCAAGCTCTC GGAAATATGT GTGTAATGAA CATGAACTAT
    TATGACTCCT CTACTTTTGA TGCATGTGGA TTGTTTCAGT TTATCTTTGA AAATACTGCT
    GCACTGGGCA CTGTTCATTC TGTCTCCTTT TGGAGACAGA ATCTTCCATG GCTGTTCTAT
    GGAGACCAGC TAGGATTAGC ACCTCAAATA CTTAGTACTA CACCTCTTCC TACAAATTTC
    AGTTTTAAAG GGGAAAACCA GCAGAGTACA AAACTGAAGT TTGTTGCTGC TTCCTATGAT
    GTGAGAGGAA ATTTTCTCAA GTGGCAAACT TTAGAAGGAG GGATTTTACA GCTTTGTCCA
    GACACAGAGA CGAGACTAAA TGCTGCTTAT TCCTTTGGAA CAACTTATCA ACAAAGTTGT
    GAGATTCCTG TCCCTAAGAT CTTAACTGAC TTTCCCACTC CTACGTTTTA TGATGTGTAC
    CTTGAATATA CTGATGAGAA TCAGCACCAA TATATTTGGG CTGTGCCTGT GTTAAACTTA
    AATCTTCAGC ACAAGAAAAT GTTTGTGAAC AAAGACAGTA GTTCTGGCAA GTGGCTTCTG
    ACTCGGCGCA TTTTCTTAGT GGATACACTA AGTGGACGGG AAAATGACTT AGGAAGCCAG
    CCAAGATTAA TTAGAGTCGC CACCCAAATA TCACTGAGCA TCCACCTTGT ACCCAACACA
    AAAAATGGAA ACATTTACCC TCCCTTAATT ACCATTGCCT ACCATGACAT TGATATCAAA
    GATCCCAGCA GCCAGTCTGT GAAGGTTTCT TTCTCAGTCA CATACGAAAT GGATCAAGGA
    GAAGCACATG TCCAGACAGA TATTGCTTTA GGTGTATTGG GTGGACTAGC TGTTTTATCA
    TCTCTTTTGA AGACAGCAGG TTGGAAGAGA CGTATTGGGA GTCCCATGAT TGATTTACAG
    GCTCTACAAT TTTTGCATAA GCTCATCTCC CAGATTACCA TAGACATATT CTTTATCGAT
    TGGGAGCGGC CTAAAGGAAA GGTTCTTAAA GCTGTTGAAG GTGAGGGTGG TGTTCGGAGT
    GCTACTGTTC CTGTAAGCAT ATGGAGAACT TATTTTGTGG CAAATGAATG GAACGAAATT
    CAGACTGTGA GAAAAATTAA CCCACTCTTT CAAGTACTTA CTGTCCTCTT CCTTTTGGAG
    GTTGTGGGAT TTAAGAACTT AGCATTAATG GACTCATCCT CTAGTCTTTC CAGAAGCCCA
    CCTAGCTACA TAGCTCCTTA CAGCCGCATT TTGAGATACG CGGTATCTTC TGCTCTTTGG
    CTGGTCATTG GACTTATACA GATTGTGTTC TTTGCCGTCT TCTATGAGAG ATTTATAGAA
    GATAAAATTC GACAGTTTGT TGATTTGTGC TGTATGAGTA ATATATCAGT GTTTCTGTTA
    TCCCACAGAT GTTTTGGCTA CTACATCCAT GGTAGATCAG TACATGGGCA TGCTGATACT
    AATATGGAAG AAATGAATAT GAATCTTAAA AGAGAAGCGG AAAATTTATG TAGCCAAAGA
    GGTTTGGTAC CTAATACAGA TGGTCAGACT TTTCAGATTG CAATTTCTAG CCAGATGAGG
    CAACACTATG ACAGAATTCA TGAGACACTA ACAAGGAAAA ATGGCCCTGC TAGACTATTG
    AGTTCCTCAG GAAGTACTTT CGAGCAGAGT GTAAAAGCAT ATCATACTAT GAATAAATTT
    CTTGGCTCTT TCATCGATCA TGTTCATAAG GAAATGGACT ACTTTATAAA AGATAAATTG
    CTTCTTGAAA GAATTCTTGG CATGGAATTC ATGGAACCAA TGGAAAAAAG CATCTTTTAC
    AATGATGAAG GATATTCTTT CAGCAGTGTT CTGTATTATG GAAATGAAGC TACTCTTCTT
    ATTTATGATC TGATGTTCTT CTGTGTTGTG GATTTGGCTT GCCAAAATTT TATTTTAGCA
    GCCTTCCTTA CATACCTACA ACAAGAGATT TTTAGATTTA TTCGTAATGC AGTAGGACAG
    AAGAATTTGG CATCCAAAAC ACTGGTGGAT CAAAGATTCC TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.