TMEM67 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM67
Entrez Gene ID	91147
Full Name	transmembrane protein 67
Synonyms	JBTS6,MECKELIN,MKS3,NPHP11,TNEM67
General protein information	Preferred Names transmembrane protein 67 Names meckelin meckel syndrome type 3 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q22.1
Summary	The protein encoded by this gene localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). [provided by RefSeq, Nov 2008].
Disorder MIM:	609884
Disorder Html:	Meckel syndrome 3, 607361 (3); Joubert syndrome 6, 610688 (3); {Bardet-Biedl syndrome 14, modifier of}, 615991 (3); COACH syndrome, 216360 (3); Nephronophthisis 11, 613550 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006716686.3	XP_006716749.1	meckelin isoform X1
NM_001142301.1	NP_001135773.1	meckelin isoform 2
XM_006716686.4	XP_006716749.1	meckelin isoform X1
XM_011517363.3	XP_011515665.1	meckelin isoform X3
NM_153704.6	NP_714915.3	meckelin isoform 1 precursor
XM_024447326.1	XP_024303094.1	meckelin isoform X2
XM_011517363.2	XP_011515665.1	meckelin isoform X2
NM_153704.5	NP_714915.3	meckelin isoform 1 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tmem67	XP_700974.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002548	XP_312391.5
Pan troglodytes (chimpanzee)	TMEM67	XP_528307.2
Caenorhabditis elegans (roundworm)	mks-3	NP_495591.2
Homo sapiens (human)	TMEM67	NP_714915.3
Mus musculus (house mouse)	Tmem67	NP_808529.2
Xenopus tropicalis (tropical clawed frog)	tmem67	XP_002934466.2
	TMEM67	XP_002805487.1
Bos taurus (cattle)	TMEM67	NP_001192228.1
Rattus norvegicus (Norway rat)	Tmem67	NP_001101386.3
Canis lupus familiaris (dog)	TMEM67	XP_544178.4
Gallus gallus (chicken)	TMEM67	XP_418334.3
Drosophila melanogaster (fruit fly)	CG15923	NP_001097846.1

Gene Ontology
Bibliography

Related articles in PubMed

Prospective Evaluation of Kidney Disease in Joubert Syndrome.
Fleming LR, Doherty DA, Parisi MA, Glass IA, Bryant J, Fischer R, Turkbey B, Choyke P, Daryanani K, Vemulapalli M, Mullikin JC, Malicdan MC, Vilboux T, Sayer JA, Gahl WA, Gunay-Aygun M
Clinical journal of the American Society of Nephrology : CJASN12(12)1962-1973(2017 Dec)

Functional validation of novel MKS3/TMEM67 mutations in COACH syndrome.
Lee SH, Nam TS, Li W, Kim JH, Yoon W, Choi YD, Kim KH, Cai H, Kim MJ, Kim C, Choy HE, Kim N, Chay KO, Kim MK, Choi SY
Scientific reports7(1)10222(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Importantly, one Japanese and one Omani families carried compound biallelic mutations in two distinct genes (TMEM67/RPGRIP1L and TMEM138/BBS1, respectively).
Title: Molecular genetic analysis of 30 families with Joubert syndrome.

The Meckel syndrome protein meckelin (TMEM67) is a key regulator of cilia function but is not required for tissue planar polarity.
Title: The Meckel syndrome protein meckelin (TMEM67) is a key regulator of cilia function but is not required for tissue planar polarity.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutation analysis of 18 nephronophthisis associated ciliopathy disease genes using a DNA pooling and next generation sequencing strategy.

mutation analysis of TMEM67 in Joubert syndrome and related disorders cases and Meckel syndrome fetuses; identification of 20 novel mutations; review of published mutations and discussion of genotype-phenotype correlates
Title: Novel TMEM67 mutations and genotype-phenotype correlates in meckelin-related ciliopathies.

Data show that knockdown of MKS3 inhibited degradation of mutant SP-C.
Title: Meckel-Gruber syndrome protein MKS3 is required for endoplasmic reticulum-associated degradation of surfactant protein C.

The following TMEM67 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM67 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26231	NM_001142301.1 Latest version!	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$643.30 $919.00
OHu42606	XM_006716686.4 Latest version!	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu74308	XM_011517363.3 Latest version!	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu26304	NM_153704.6 Latest version!	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu106073	XM_024447326.1 Latest version!	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu42606	XM_006716686.3	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu74308	XM_011517363.2	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu26304	NM_153704.5	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM67 ( NM_001142301.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135773.1 Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26231
Clone ID Related Accession (Same CDS sequence)	NM_001142301.1
Accession Version	NM_001142301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	meckelin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC368894.1, AK092244.1, AC010834.19, BC032835.2 and BM310097.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) has an alternate splice pattern in the 5' coding region. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092244.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCACTGT CCCATTGGCC ATATTTTAGG CTGGTCTTGA ACTTCCGACC TCAGGTGATC 
TGCCTGCCTC AGCCTCCCAA AGTGCTGGGA TACAGGCTTG AGCCACCGCA CCTGACCTTG 
GCATGTACTT TAGAAGGAAT GTACAACATA ATTGAAGAAA TTTTGGAAAG AGACATTAAT 
GGAACATTGT TGTCTCAAGC AACTTGTGAG CTCTGTGATG GAAATGAAAA CTCTTTTATG 
GTAGTAAATG CTTTAGGAGA CAGGTGCGTC CGATGTGAGC CAACATTTGT TAATACCAGC 
AGGTCCTGTG CATGTTCAGA ACCTAACATT TTAACAGGGG GATTATGTTT CAGCAGCACA 
GGGAATTTTC CTCTACGTAG AATTTCAGCT GCACGTTATG GAGAAGTTGG CATGTCTTTA 
ACTTCAGAAT GGTTTGCAAA GTATTTGCAA TCATCAGCAG CTGCATGTTG GGTATATGCC 
AATCTAACAT CTTGTCAAGC TCTTGGAAAT ATGTGTGTGA TGAACATGAA TTCTTACGAC 
TTTGCCACAT TTGATGCATG TGGACTATTT CAGTTTATCT TTGAAAATAC TGCTGGACTG 
AGCACTGTTC ATTCTATTTC ATTTTGGAGA CAGAATCTTC CTTGGCTGTT TTATGGAGAC 
CAGTTAGGAT TAGCACCTCA AGTGCTCAGT TCTACCTCTC TTCCTACAAA TTTCAGTTTT 
AAAGGAGAAA ACCAGAATAC AAAACTGAAG TTTGTTGCTG CTTCCTATGA TATAAGAGGA 
AATTTTCTCA AGTGGCAAAC TTTAGAAGGA GGTGTTTTAC AGCTTTGTCC AGACACAGAG 
ACAAGGCTAA ATGCTGCTTA TTCATTTGGA ACAACCTACC AACAAAATTG TGAGATTCCT 
ATCTCTAAGA TCTTAATTGA CTTTCCCACT CCTATATTTT ATGATGTGTA CCTTGAATAT 
ACTGATGAAA ATCAACATCA ATATATTTTG GCTGTGCCTG TGTTAAACCT AAATCTTCAA 
CATAATAAGA TATTTGTGAA CCAAGACAGC AACTCTGGAA AGTGGCTTCT AACTCGGCGC 
ATTTTCTTAG TGGATGCAGT AAGTGGACGA GAAAATGACT TAGGAACTCA GCCAAGAGTA 
ATTCGAGTTG CTACTCAAAT ATCACTGAGT GTCCACCTTG TACCCAACAC AATAAATGGA 
AACATCTACC CTCCCTTAAT CACCATTGCC TACAGTGACA TTGATATCAA AGATGCCAAC 
AGCCAGTCTG TGAAGGTTTC TTTCTCAGTC ACATATGAAA TGGATCATGG AGAAGCACAT 
GTCCAGACAG ATATTGCTTT GGGTGTATTG GGTGGGCTAG CTGTTTTAGC ATCTCTTTTG 
AAGACAGCAG GATGGAAGAG GCGCATTGGG AGTCCCATGA TTGATTTACA GACAGTTGTG 
AAATTCTTGG TGTACTATGC TGGTGATCTG GCCAATGTTT TCTTTATCAT CACAGTGGGA 
ACAGGTCTTT ACTGGCTTAT TTTCTTCAAA GCACAGAAGT CTGTGTCTGT TTTGCTGCCA 
ATGCCAATTC AGGAAGAACG TTTTGTCACT TATGTTGGAT GTGCCTTTGC TCTGAAGGCA 
CTACAATTTT TGCATAAGCT CATATCCCAG ATTACAATAG ATGTATTCTT TATTGATTGG 
GAGCGACCTA AAGGAAAGGT TCTTAAAGCT GTTGAAGGTG AGGGTGGTGT ACGAAGTGCC 
ACTGTTCCTG TAAGCATATG GAGAACATAT TTTGTAGCAA ATGAATGGAA TGAAATTCAG 
ACTGTGAGAA AAATTAATTC ACTCTTTCAA GTACTTACTG TCCTCTTCTT TTTGGAGGTT 
GTGGGATTCA AGAACTTAGC ATTAATGGAC TCATCTTCTA GTCTTTCTAG AAACCCACCT 
AGCTACATAG CTCCTTATAG CTGCATTTTG AGATATGCAG TGTCTGCTGC TCTTTGGCTA 
GCCATTGGAA TTATACAGGT CGTGTTCTTT GCTGTCTTTT ATGAGAGATT TATAGAAGAT 
AAAATTCGAC AGTTCGTTGA TTTATGCTCT ATGAGTAATA TATCAGTGTT TCTGTTATCC 
CACAAATGTT TTGGATATTA CATTCATGGT AGATCAGTAC ATGGGCATGC AGATACTAAT 
ATGGAAGAAA TGAATATGAA CCTTAAAAGA GAAGCGGAAA ATTTGTGTAG CCAGAGAGGT 
TTGGTACCCA ACACAGATGG TCAGACTTTT GAGATTGCAA TTTCTAACCA GATGAGACAA 
CATTATGACA GAATTCATGA GACACTAATA AGGAAAAATG GTCCTGCTAG ACTACTGAGT 
TCATCAGCAA GTACTTTTGA GCAGAGTATA AAAGCATATC ATATGATGAA TAAATTTCTT 
GGCTCCTTCA TTGACCATGT TCATAAGGAA ATGGATTACT TTATAAAAGA TAAGTTGCTT 
CTTGAAAGAA TTCTTGGAAT GGAATTCATG GAACCAATGG AAAAAAGCAT CTTTTACAAT 
GATGAAGGTT ATTCTTTCAG CAGTGTCCTG TATTATGGAA ATGAAGCTAC TCTTCTTATT 
TTTGATCTGC TGTTCTTCTG TGTTGTGGAT TTGGCTTGCC AAAATTTTAT TTTAGCATCC 
TTCCTTACAT ATCTACAACA AGAGATTTTT AGATATATCC GTAATACAGT AGGACAAAAG 
AATTTGGCAT CCAAAACATT GGTGGATCAA AGATTTTTGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135773.1
CDS	246..2990
Translation	MSLSHWPYFRLVLNFRPQVICLPQPPKVLGYRLEPPHLTLACTLEGMYNIIEEILERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTFVNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQTLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTPIFYDVYLEYTDENQHQYILAVPVLNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI

Target ORF information:

RefSeq Version	NM_001142301.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142301.1

ORF Insert Sequence:

ATGTCACTGT CCCATTGGCC ATATTTTAGG CTGGTCTTGA ACTTCCGACC TCAGGTGATC 
TGCCTGCCTC AGCCTCCCAA AGTGCTGGGA TACAGGCTTG AGCCACCGCA CCTGACCTTG 
GCATGTACTT TAGAAGGAAT GTACAACATA ATTGAAGAAA TTTTGGAAAG AGACATTAAT 
GGAACATTGT TGTCTCAAGC AACTTGTGAG CTCTGTGATG GAAATGAAAA CTCTTTTATG 
GTAGTAAATG CTTTAGGAGA CAGGTGCGTC CGATGTGAGC CAACATTTGT TAATACCAGC 
AGGTCCTGTG CATGTTCAGA ACCTAACATT TTAACAGGGG GATTATGTTT CAGCAGCACA 
GGGAATTTTC CTCTACGTAG AATTTCAGCT GCACGTTATG GAGAAGTTGG CATGTCTTTA 
ACTTCAGAAT GGTTTGCAAA GTATTTGCAA TCATCAGCAG CTGCATGTTG GGTATATGCC 
AATCTAACAT CTTGTCAAGC TCTTGGAAAT ATGTGTGTGA TGAACATGAA TTCTTACGAC 
TTTGCCACAT TTGATGCATG TGGACTATTT CAGTTTATCT TTGAAAATAC TGCTGGACTG 
AGCACTGTTC ATTCTATTTC ATTTTGGAGA CAGAATCTTC CTTGGCTGTT TTATGGAGAC 
CAGTTAGGAT TAGCACCTCA AGTGCTCAGT TCTACCTCTC TTCCTACAAA TTTCAGTTTT 
AAAGGAGAAA ACCAGAATAC AAAACTGAAG TTTGTTGCTG CTTCCTATGA TATAAGAGGA 
AATTTTCTCA AGTGGCAAAC TTTAGAAGGA GGTGTTTTAC AGCTTTGTCC AGACACAGAG 
ACAAGGCTAA ATGCTGCTTA TTCATTTGGA ACAACCTACC AACAAAATTG TGAGATTCCT 
ATCTCTAAGA TCTTAATTGA CTTTCCCACT CCTATATTTT ATGATGTGTA CCTTGAATAT 
ACTGATGAAA ATCAACATCA ATATATTTTG GCTGTGCCTG TGTTAAACCT AAATCTTCAA 
CATAATAAGA TATTTGTGAA CCAAGACAGC AACTCTGGAA AGTGGCTTCT AACTCGGCGC 
ATTTTCTTAG TGGATGCAGT AAGTGGACGA GAAAATGACT TAGGAACTCA GCCAAGAGTA 
ATTCGAGTTG CTACTCAAAT ATCACTGAGT GTCCACCTTG TACCCAACAC AATAAATGGA 
AACATCTACC CTCCCTTAAT CACCATTGCC TACAGTGACA TTGATATCAA AGATGCCAAC 
AGCCAGTCTG TGAAGGTTTC TTTCTCAGTC ACATATGAAA TGGATCATGG AGAAGCACAT 
GTCCAGACAG ATATTGCTTT GGGTGTATTG GGTGGGCTAG CTGTTTTAGC ATCTCTTTTG 
AAGACAGCAG GATGGAAGAG GCGCATTGGG AGTCCCATGA TTGATTTACA GACAGTTGTG 
AAATTCTTGG TGTACTATGC TGGTGATCTG GCCAATGTTT TCTTTATCAT CACAGTGGGA 
ACAGGTCTTT ACTGGCTTAT TTTCTTCAAA GCACAGAAGT CTGTGTCTGT TTTGCTGCCA 
ATGCCAATTC AGGAAGAACG TTTTGTCACT TATGTTGGAT GTGCCTTTGC TCTGAAGGCA 
CTACAATTTT TGCATAAGCT CATATCCCAG ATTACAATAG ATGTATTCTT TATTGATTGG 
GAGCGACCTA AAGGAAAGGT TCTTAAAGCT GTTGAAGGTG AGGGTGGTGT ACGAAGTGCC 
ACTGTTCCTG TAAGCATATG GAGAACATAT TTTGTAGCAA ATGAATGGAA TGAAATTCAG 
ACTGTGAGAA AAATTAATTC ACTCTTTCAA GTACTTACTG TCCTCTTCTT TTTGGAGGTT 
GTGGGATTCA AGAACTTAGC ATTAATGGAC TCATCTTCTA GTCTTTCTAG AAACCCACCT 
AGCTACATAG CTCCTTATAG CTGCATTTTG AGATATGCAG TGTCTGCTGC TCTTTGGCTA 
GCCATTGGAA TTATACAGGT CGTGTTCTTT GCTGTCTTTT ATGAGAGATT TATAGAAGAT 
AAAATTCGAC AGTTCGTTGA TTTATGCTCT ATGAGTAATA TATCAGTGTT TCTGTTATCC 
CACAAATGTT TTGGATATTA CATTCATGGT AGATCAGTAC ATGGGCATGC AGATACTAAT 
ATGGAAGAAA TGAATATGAA CCTTAAAAGA GAAGCGGAAA ATTTGTGTAG CCAGAGAGGT 
TTGGTACCCA ACACAGATGG TCAGACTTTT GAGATTGCAA TTTCTAACCA GATGAGACAA 
CATTATGACA GAATTCATGA GACACTAATA AGGAAAAATG GTCCTGCTAG ACTACTGAGT 
TCATCAGCAA GTACTTTTGA GCAGAGTATA AAAGCATATC ATATGATGAA TAAATTTCTT 
GGCTCCTTCA TTGACCATGT TCATAAGGAA ATGGATTACT TTATAAAAGA TAAGTTGCTT 
CTTGAAAGAA TTCTTGGAAT GGAATTCATG GAACCAATGG AAAAAAGCAT CTTTTACAAT 
GATGAAGGTT ATTCTTTCAG CAGTGTCCTG TATTATGGAA ATGAAGCTAC TCTTCTTATT 
TTTGATCTGC TGTTCTTCTG TGTTGTGGAT TTGGCTTGCC AAAATTTTAT TTTAGCATCC 
TTCCTTACAT ATCTACAACA AGAGATTTTT AGATATATCC GTAATACAGT AGGACAAAAG 
AATTTGGCAT CCAAAACATT GGTGGATCAA AGATTTTTGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM67 ( NM_153704.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_714915.3 Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26304
Clone ID Related Accession (Same CDS sequence)	NM_153704.6 , NM_153704.5
Accession Version	NM_153704.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	meckelin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK308071.1, BC031220.1, BC054338.1, AC010834.19 and BM310097.1. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2008 this sequence version replaced NM_153704.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.128946.1, SRR1803616.12425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGC GCGGTGGGGC TGGGGTGGCA ATGGCGGTTT GGTCCCTCTT ATCCGCCCGG 
GCCGTGACCG CGTTCCTTCT GTTGTTCCTC CCTCGCTTCT TACAGGCCCA GACCTTCTCT 
TTCCCTTTCC AGCAGCCGGA GAAGTGCGAC AACAACCAGT ACTTTGATAT CTCCGCCCTC 
TCGTGTGTTC CTTGTGGAGC TAACCAGAGG CAAGATGCCC GAGGAACTTC ATGTGTATGT 
CTACCAGGAT TTCAGATGAT CTCTAATAAT GGAGGACCTG CTATTATTTG TAAAAAGTGC 
CCAGAAAACA TGAAAGGTGT TACAGAAGAT GGCTGGAACT GCATTTCTTG CCCTAGTGAC 
TTAACTGCCG AAGGAAAATG TCACTGTCCC ATTGGCCATA TTTTAGTGGA AAGAGACATT 
AATGGAACAT TGTTGTCTCA AGCAACTTGT GAGCTCTGTG ATGGAAATGA AAACTCTTTT 
ATGGTAGTAA ATGCTTTAGG AGACAGGTGC GTCCGATGTG AGCCAACATT TGTTAATACC 
AGCAGGTCCT GTGCATGTTC AGAACCTAAC ATTTTAACAG GGGGATTATG TTTCAGCAGC 
ACAGGGAATT TTCCTCTACG TAGAATTTCA GCTGCACGTT ATGGAGAAGT TGGCATGTCT 
TTAACTTCAG AATGGTTTGC AAAGTATTTG CAATCATCAG CAGCTGCATG TTGGGTATAT 
GCCAATCTAA CATCTTGTCA AGCTCTTGGA AATATGTGTG TGATGAACAT GAATTCTTAC 
GACTTTGCCA CATTTGATGC ATGTGGACTA TTTCAGTTTA TCTTTGAAAA TACTGCTGGA 
CTGAGCACTG TTCATTCTAT TTCATTTTGG AGACAGAATC TTCCTTGGCT GTTTTATGGA 
GACCAGTTAG GATTAGCACC TCAAGTGCTC AGTTCTACCT CTCTTCCTAC AAATTTCAGT 
TTTAAAGGAG AAAACCAGAA TACAAAACTG AAGTTTGTTG CTGCTTCCTA TGATATAAGA 
GGAAATTTTC TCAAGTGGCA AACTTTAGAA GGAGGTGTTT TACAGCTTTG TCCAGACACA 
GAGACAAGGC TAAATGCTGC TTATTCATTT GGAACAACCT ACCAACAAAA TTGTGAGATT 
CCTATCTCTA AGATCTTAAT TGACTTTCCC ACTCCTATAT TTTATGATGT GTACCTTGAA 
TATACTGATG AAAATCAACA TCAATATATT TTGGCTGTGC CTGTGTTAAA CCTAAATCTT 
CAACATAATA AGATATTTGT GAACCAAGAC AGCAACTCTG GAAAGTGGCT TCTAACTCGG 
CGCATTTTCT TAGTGGATGC AGTAAGTGGA CGAGAAAATG ACTTAGGAAC TCAGCCAAGA 
GTAATTCGAG TTGCTACTCA AATATCACTG AGTGTCCACC TTGTACCCAA CACAATAAAT 
GGAAACATCT ACCCTCCCTT AATCACCATT GCCTACAGTG ACATTGATAT CAAAGATGCC 
AACAGCCAGT CTGTGAAGGT TTCTTTCTCA GTCACATATG AAATGGATCA TGGAGAAGCA 
CATGTCCAGA CAGATATTGC TTTGGGTGTA TTGGGTGGGC TAGCTGTTTT AGCATCTCTT 
TTGAAGACAG CAGGATGGAA GAGGCGCATT GGGAGTCCCA TGATTGATTT ACAGACAGTT 
GTGAAATTCT TGGTGTACTA TGCTGGTGAT CTGGCCAATG TTTTCTTTAT CATCACAGTG 
GGAACAGGTC TTTACTGGCT TATTTTCTTC AAAGCACAGA AGTCTGTGTC TGTTTTGCTG 
CCAATGCCAA TTCAGGAAGA ACGTTTTGTC ACTTATGTTG GATGTGCCTT TGCTCTGAAG 
GCACTACAAT TTTTGCATAA GCTCATATCC CAGATTACAA TAGATGTATT CTTTATTGAT 
TGGGAGCGAC CTAAAGGAAA GGTTCTTAAA GCTGTTGAAG GTGAGGGTGG TGTACGAAGT 
GCCACTGTTC CTGTAAGCAT ATGGAGAACA TATTTTGTAG CAAATGAATG GAATGAAATT 
CAGACTGTGA GAAAAATTAA TTCACTCTTT CAAGTACTTA CTGTCCTCTT CTTTTTGGAG 
GTTGTGGGAT TCAAGAACTT AGCATTAATG GACTCATCTT CTAGTCTTTC TAGAAACCCA 
CCTAGCTACA TAGCTCCTTA TAGCTGCATT TTGAGATATG CAGTGTCTGC TGCTCTTTGG 
CTAGCCATTG GAATTATACA GGTCGTGTTC TTTGCTGTCT TTTATGAGAG ATTTATAGAA 
GATAAAATTC GACAGTTCGT TGATTTATGC TCTATGAGTA ATATATCAGT GTTTCTGTTA 
TCCCACAAAT GTTTTGGATA TTACATTCAT GGTAGATCAG TACATGGGCA TGCAGATACT 
AATATGGAAG AAATGAATAT GAACCTTAAA AGAGAAGCGG AAAATTTGTG TAGCCAGAGA 
GGTTTGGTAC CCAACACAGA TGGTCAGACT TTTGAGATTG CAATTTCTAA CCAGATGAGA 
CAACATTATG ACAGAATTCA TGAGACACTA ATAAGGAAAA ATGGTCCTGC TAGACTACTG 
AGTTCATCAG CAAGTACTTT TGAGCAGAGT ATAAAAGCAT ATCATATGAT GAATAAATTT 
CTTGGCTCCT TCATTGACCA TGTTCATAAG GAAATGGATT ACTTTATAAA AGATAAGTTG 
CTTCTTGAAA GAATTCTTGG AATGGAATTC ATGGAACCAA TGGAAAAAAG CATCTTTTAC 
AATGATGAAG GTTATTCTTT CAGCAGTGTC CTGTATTATG GAAATGAAGC TACTCTTCTT 
ATTTTTGATC TGCTGTTCTT CTGTGTTGTG GATTTGGCTT GCCAAAATTT TATTTTAGCA 
TCCTTCCTTA CATATCTACA ACAAGAGATT TTTAGATATA TCCGTAATAC AGTAGGACAA 
AAGAATTTGG CATCCAAAAC ATTGGTGGAT CAAAGATTTT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_714915.3
CDS	72..3059
Translation	MATRGGAGVAMAVWSLLSARAVTAFLLLFLPRFLQAQTFSFPFQQPEKCDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTFVNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQTLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTPIFYDVYLEYTDENQHQYILAVPVLNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI

Target ORF information:

RefSeq Version	NM_153704.5
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153704.5

ORF Insert Sequence:

ATGGCGACGC GCGGTGGGGC TGGGGTGGCA ATGGCGGTTT GGTCCCTCTT ATCCGCCCGG 
GCCGTGACCG CGTTCCTTCT GTTGTTCCTC CCTCGCTTCT TACAGGCCCA GACCTTCTCT 
TTCCCTTTCC AGCAGCCGGA GAAGTGCGAC AACAACCAGT ACTTTGATAT CTCCGCCCTC 
TCGTGTGTTC CTTGTGGAGC TAACCAGAGG CAAGATGCCC GAGGAACTTC ATGTGTATGT 
CTACCAGGAT TTCAGATGAT CTCTAATAAT GGAGGACCTG CTATTATTTG TAAAAAGTGC 
CCAGAAAACA TGAAAGGTGT TACAGAAGAT GGCTGGAACT GCATTTCTTG CCCTAGTGAC 
TTAACTGCCG AAGGAAAATG TCACTGTCCC ATTGGCCATA TTTTAGTGGA AAGAGACATT 
AATGGAACAT TGTTGTCTCA AGCAACTTGT GAGCTCTGTG ATGGAAATGA AAACTCTTTT 
ATGGTAGTAA ATGCTTTAGG AGACAGGTGC GTCCGATGTG AGCCAACATT TGTTAATACC 
AGCAGGTCCT GTGCATGTTC AGAACCTAAC ATTTTAACAG GGGGATTATG TTTCAGCAGC 
ACAGGGAATT TTCCTCTACG TAGAATTTCA GCTGCACGTT ATGGAGAAGT TGGCATGTCT 
TTAACTTCAG AATGGTTTGC AAAGTATTTG CAATCATCAG CAGCTGCATG TTGGGTATAT 
GCCAATCTAA CATCTTGTCA AGCTCTTGGA AATATGTGTG TGATGAACAT GAATTCTTAC 
GACTTTGCCA CATTTGATGC ATGTGGACTA TTTCAGTTTA TCTTTGAAAA TACTGCTGGA 
CTGAGCACTG TTCATTCTAT TTCATTTTGG AGACAGAATC TTCCTTGGCT GTTTTATGGA 
GACCAGTTAG GATTAGCACC TCAAGTGCTC AGTTCTACCT CTCTTCCTAC AAATTTCAGT 
TTTAAAGGAG AAAACCAGAA TACAAAACTG AAGTTTGTTG CTGCTTCCTA TGATATAAGA 
GGAAATTTTC TCAAGTGGCA AACTTTAGAA GGAGGTGTTT TACAGCTTTG TCCAGACACA 
GAGACAAGGC TAAATGCTGC TTATTCATTT GGAACAACCT ACCAACAAAA TTGTGAGATT 
CCTATCTCTA AGATCTTAAT TGACTTTCCC ACTCCTATAT TTTATGATGT GTACCTTGAA 
TATACTGATG AAAATCAACA TCAATATATT TTGGCTGTGC CTGTGTTAAA CCTAAATCTT 
CAACATAATA AGATATTTGT GAACCAAGAC AGCAACTCTG GAAAGTGGCT TCTAACTCGG 
CGCATTTTCT TAGTGGATGC AGTAAGTGGA CGAGAAAATG ACTTAGGAAC TCAGCCAAGA 
GTAATTCGAG TTGCTACTCA AATATCACTG AGTGTCCACC TTGTACCCAA CACAATAAAT 
GGAAACATCT ACCCTCCCTT AATCACCATT GCCTACAGTG ACATTGATAT CAAAGATGCC 
AACAGCCAGT CTGTGAAGGT TTCTTTCTCA GTCACATATG AAATGGATCA TGGAGAAGCA 
CATGTCCAGA CAGATATTGC TTTGGGTGTA TTGGGTGGGC TAGCTGTTTT AGCATCTCTT 
TTGAAGACAG CAGGATGGAA GAGGCGCATT GGGAGTCCCA TGATTGATTT ACAGACAGTT 
GTGAAATTCT TGGTGTACTA TGCTGGTGAT CTGGCCAATG TTTTCTTTAT CATCACAGTG 
GGAACAGGTC TTTACTGGCT TATTTTCTTC AAAGCACAGA AGTCTGTGTC TGTTTTGCTG 
CCAATGCCAA TTCAGGAAGA ACGTTTTGTC ACTTATGTTG GATGTGCCTT TGCTCTGAAG 
GCACTACAAT TTTTGCATAA GCTCATATCC CAGATTACAA TAGATGTATT CTTTATTGAT 
TGGGAGCGAC CTAAAGGAAA GGTTCTTAAA GCTGTTGAAG GTGAGGGTGG TGTACGAAGT 
GCCACTGTTC CTGTAAGCAT ATGGAGAACA TATTTTGTAG CAAATGAATG GAATGAAATT 
CAGACTGTGA GAAAAATTAA TTCACTCTTT CAAGTACTTA CTGTCCTCTT CTTTTTGGAG 
GTTGTGGGAT TCAAGAACTT AGCATTAATG GACTCATCTT CTAGTCTTTC TAGAAACCCA 
CCTAGCTACA TAGCTCCTTA TAGCTGCATT TTGAGATATG CAGTGTCTGC TGCTCTTTGG 
CTAGCCATTG GAATTATACA GGTCGTGTTC TTTGCTGTCT TTTATGAGAG ATTTATAGAA 
GATAAAATTC GACAGTTCGT TGATTTATGC TCTATGAGTA ATATATCAGT GTTTCTGTTA 
TCCCACAAAT GTTTTGGATA TTACATTCAT GGTAGATCAG TACATGGGCA TGCAGATACT 
AATATGGAAG AAATGAATAT GAACCTTAAA AGAGAAGCGG AAAATTTGTG TAGCCAGAGA 
GGTTTGGTAC CCAACACAGA TGGTCAGACT TTTGAGATTG CAATTTCTAA CCAGATGAGA 
CAACATTATG ACAGAATTCA TGAGACACTA ATAAGGAAAA ATGGTCCTGC TAGACTACTG 
AGTTCATCAG CAAGTACTTT TGAGCAGAGT ATAAAAGCAT ATCATATGAT GAATAAATTT 
CTTGGCTCCT TCATTGACCA TGTTCATAAG GAAATGGATT ACTTTATAAA AGATAAGTTG 
CTTCTTGAAA GAATTCTTGG AATGGAATTC ATGGAACCAA TGGAAAAAAG CATCTTTTAC 
AATGATGAAG GTTATTCTTT CAGCAGTGTC CTGTATTATG GAAATGAAGC TACTCTTCTT 
ATTTTTGATC TGCTGTTCTT CTGTGTTGTG GATTTGGCTT GCCAAAATTT TATTTTAGCA 
TCCTTCCTTA CATATCTACA ACAAGAGATT TTTAGATATA TCCGTAATAC AGTAGGACAA 
AAGAATTTGG CATCCAAAAC ATTGGTGGAT CAAAGATTTT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM67 ( XM_006716686.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006716749.1 Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu42606
Clone ID Related Accession (Same CDS sequence)	XM_006716686.3 , XM_006716686.4
Accession Version	XM_006716686.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	meckelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAGA AAGGTGTTAC AGAAGATGGC TGGAACTGCA TTTCTTGCCC TAGTGACTTA 
ACTGCCGAAG GAAAATGTCA CTGTCCCATT GGCCATATTT TAGTGGAAAG AGACATTAAT 
GGAACATTGT TGTCTCAAGC AACTTGTGAG CTCTGTGATG GAAATGAAAA CTCTTTTATG 
GTAGTAAATG CTTTAGGAGA CAGGTGCGTC CGATGTGAGC CAACATTTGT TAATACCAGC 
AGGTCCTGTG CATGTTCAGA ACCTAACATT TTAACAGGGG GATTATGTTT CAGCAGCACA 
GGGAATTTTC CTCTACGTAG AATTTCAGCT GCACGTTATG GAGAAGTTGG CATGTCTTTA 
ACTTCAGAAT GGTTTGCAAA GTATTTGCAA TCATCAGCAG CTGCATGTTG GGTATATGCC 
AATCTAACAT CTTGTCAAGC TCTTGGAAAT ATGTGTGTGA TGAACATGAA TTCTTACGAC 
TTTGCCACAT TTGATGCATG TGGACTATTT CAGTTTATCT TTGAAAATAC TGCTGGACTG 
AGCACTGTTC ATTCTATTTC ATTTTGGAGA CAGAATCTTC CTTGGCTGTT TTATGGAGAC 
CAGTTAGGAT TAGCACCTCA AGTGCTCAGT TCTACCTCTC TTCCTACAAA TTTCAGTTTT 
AAAGGAGAAA ACCAGAATAC AAAACTGAAG TTTGTTGCTG CTTCCTATGA TATAAGAGGA 
AATTTTCTCA AGTGGCAAAC TTTAGAAGGA GGTGTTTTAC AGCTTTGTCC AGACACAGAG 
ACAAGGCTAA ATGCTGCTTA TTCATTTGGA ACAACCTACC AACAAAATTG TGAGATTCCT 
ATCTCTAAGA TCTTAATTGA CTTTCCCACT CCTATATTTT ATGATGTGTA CCTTGAATAT 
ACTGATGAAA ATCAACATCA ATATATTTTG GCTGTGCCTG TGTTAAACCT AAATCTTCAA 
CATAATAAGA TATTTGTGAA CCAAGACAGC AACTCTGGAA AGTGGCTTCT AACTCGGCGC 
ATTTTCTTAG TGGATGCAGT AAGTGGACGA GAAAATGACT TAGGAACTCA GCCAAGAGTA 
ATTCGAGTTG CTACTCAAAT ATCACTGAGT GTCCACCTTG TACCCAACAC AATAAATGGA 
AACATCTACC CTCCCTTAAT CACCATTGCC TACAGTGACA TTGATATCAA AGATGCCAAC 
AGCCAGTCTG TGAAGGTTTC TTTCTCAGTC ACATATGAAA TGGATCATGG AGAAGCACAT 
GTCCAGACAG ATATTGCTTT GGGTGTATTG GGTGGGCTAG CTGTTTTAGC ATCTCTTTTG 
AAGACAGCAG GATGGAAGAG GCGCATTGGG AGTCCCATGA TTGATTTACA GACAGTTGTG 
AAATTCTTGG TGTACTATGC TGGTGATCTG GCCAATGTTT TCTTTATCAT CACAGTGGGA 
ACAGGTCTTT ACTGGCTTAT TTTCTTCAAA GCACAGAAGT CTGTGTCTGT TTTGCTGCCA 
ATGCCAATTC AGGAAGAACG TTTTGTCACT TATGTTGGAT GTGCCTTTGC TCTGAAGGCA 
CTACAATTTT TGCATAAGCT CATATCCCAG ATTACAATAG ATGTATTCTT TATTGATTGG 
GAGCGACCTA AAGGAAAGGT TCTTAAAGCT GTTGAAGGTG AGGGTGGTGT ACGAAGTGCC 
ACTGTTCCTG TAAGCATATG GAGAACATAT TTTGTAGCAA ATGAATGGAA TGAAATTCAG 
ACTGTGAGAA AAATTAATTC ACTCTTTCAA GTACTTACTG TCCTCTTCTT TTTGGAGGTT 
GTGGGATTCA AGAACTTAGC ATTAATGGAC TCATCTTCTA GTCTTTCTAG AAACCCACCT 
AGCTACATAG CTCCTTATAG CTGCATTTTG AGATATGCAG TGTCTGCTGC TCTTTGGCTA 
GCCATTGGAA TTATACAGGT CGTGTTCTTT GCTGTCTTTT ATGAGAGATT TATAGAAGAT 
AAAATTCGAC AGTTCGTTGA TTTATGCTCT ATGAGTAATA TATCAGTGTT TCTGTTATCC 
CACAAATGTT TTGGATATTA CATTCATGGT AGATCAGTAC ATGGGCATGC AGATACTAAT 
ATGGAAGAAA TGAATATGAA CCTTAAAAGA GAAGCGGAAA ATTTGTGTAG CCAGAGAGGT 
TTGGTACCCA ACACAGATGG TCAGACTTTT GAGATTGCAA TTTCTAACCA GATGAGACAA 
CATTATGACA GAATTCATGA GACACTAATA AGGAAAAATG GTCCTGCTAG ACTACTGAGT 
TCATCAGCAA GTACTTTTGA GCAGAGTATA AAAGCATATC ATATGATGAA TAAATTTCTT 
GGCTCCTTCA TTGACCATGT TCATAAGGAA ATGGATTACT TTATAAAAGA TAAGTTGCTT 
CTTGAAAGAA TTCTTGGAAT GGAATTCATG GAACCAATGG AAAAAAGCAT CTTTTACAAT 
GATGAAGGTT ATTCTTTCAG CAGTGTCCTG TATTATGGAA ATGAAGCTAC TCTTCTTATT 
TTTGATCTGC TGTTCTTCTG TGTTGTGGAT TTGGCTTGCC AAAATTTTAT TTTAGCATCC 
TTCCTTACAT ATCTACAACA AGAGATTTTT AGATATATCC GTAATACAGT AGGACAAAAG 
AATTTGGCAT CCAAAACATT GGTGGATCAA AGATTTTTGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006716749.1
CDS	213..2897
Translation	MPEKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTFVNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQTLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTPIFYDVYLEYTDENQHQYILAVPVLNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI

Target ORF information:

RefSeq Version	XM_006716686.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716686.3

ORF Insert Sequence:

ATGCCCGAGA AAGGTGTTAC AGAAGATGGC TGGAACTGCA TTTCTTGCCC TAGTGACTTA 
ACTGCCGAAG GAAAATGTCA CTGTCCCATT GGCCATATTT TAGTGGAAAG AGACATTAAT 
GGAACATTGT TGTCTCAAGC AACTTGTGAG CTCTGTGATG GAAATGAAAA CTCTTTTATG 
GTAGTAAATG CTTTAGGAGA CAGGTGCGTC CGATGTGAGC CAACATTTGT TAATACCAGC 
AGGTCCTGTG CATGTTCAGA ACCTAACATT TTAACAGGGG GATTATGTTT CAGCAGCACA 
GGGAATTTTC CTCTACGTAG AATTTCAGCT GCACGTTATG GAGAAGTTGG CATGTCTTTA 
ACTTCAGAAT GGTTTGCAAA GTATTTGCAA TCATCAGCAG CTGCATGTTG GGTATATGCC 
AATCTAACAT CTTGTCAAGC TCTTGGAAAT ATGTGTGTGA TGAACATGAA TTCTTACGAC 
TTTGCCACAT TTGATGCATG TGGACTATTT CAGTTTATCT TTGAAAATAC TGCTGGACTG 
AGCACTGTTC ATTCTATTTC ATTTTGGAGA CAGAATCTTC CTTGGCTGTT TTATGGAGAC 
CAGTTAGGAT TAGCACCTCA AGTGCTCAGT TCTACCTCTC TTCCTACAAA TTTCAGTTTT 
AAAGGAGAAA ACCAGAATAC AAAACTGAAG TTTGTTGCTG CTTCCTATGA TATAAGAGGA 
AATTTTCTCA AGTGGCAAAC TTTAGAAGGA GGTGTTTTAC AGCTTTGTCC AGACACAGAG 
ACAAGGCTAA ATGCTGCTTA TTCATTTGGA ACAACCTACC AACAAAATTG TGAGATTCCT 
ATCTCTAAGA TCTTAATTGA CTTTCCCACT CCTATATTTT ATGATGTGTA CCTTGAATAT 
ACTGATGAAA ATCAACATCA ATATATTTTG GCTGTGCCTG TGTTAAACCT AAATCTTCAA 
CATAATAAGA TATTTGTGAA CCAAGACAGC AACTCTGGAA AGTGGCTTCT AACTCGGCGC 
ATTTTCTTAG TGGATGCAGT AAGTGGACGA GAAAATGACT TAGGAACTCA GCCAAGAGTA 
ATTCGAGTTG CTACTCAAAT ATCACTGAGT GTCCACCTTG TACCCAACAC AATAAATGGA 
AACATCTACC CTCCCTTAAT CACCATTGCC TACAGTGACA TTGATATCAA AGATGCCAAC 
AGCCAGTCTG TGAAGGTTTC TTTCTCAGTC ACATATGAAA TGGATCATGG AGAAGCACAT 
GTCCAGACAG ATATTGCTTT GGGTGTATTG GGTGGGCTAG CTGTTTTAGC ATCTCTTTTG 
AAGACAGCAG GATGGAAGAG GCGCATTGGG AGTCCCATGA TTGATTTACA GACAGTTGTG 
AAATTCTTGG TGTACTATGC TGGTGATCTG GCCAATGTTT TCTTTATCAT CACAGTGGGA 
ACAGGTCTTT ACTGGCTTAT TTTCTTCAAA GCACAGAAGT CTGTGTCTGT TTTGCTGCCA 
ATGCCAATTC AGGAAGAACG TTTTGTCACT TATGTTGGAT GTGCCTTTGC TCTGAAGGCA 
CTACAATTTT TGCATAAGCT CATATCCCAG ATTACAATAG ATGTATTCTT TATTGATTGG 
GAGCGACCTA AAGGAAAGGT TCTTAAAGCT GTTGAAGGTG AGGGTGGTGT ACGAAGTGCC 
ACTGTTCCTG TAAGCATATG GAGAACATAT TTTGTAGCAA ATGAATGGAA TGAAATTCAG 
ACTGTGAGAA AAATTAATTC ACTCTTTCAA GTACTTACTG TCCTCTTCTT TTTGGAGGTT 
GTGGGATTCA AGAACTTAGC ATTAATGGAC TCATCTTCTA GTCTTTCTAG AAACCCACCT 
AGCTACATAG CTCCTTATAG CTGCATTTTG AGATATGCAG TGTCTGCTGC TCTTTGGCTA 
GCCATTGGAA TTATACAGGT CGTGTTCTTT GCTGTCTTTT ATGAGAGATT TATAGAAGAT 
AAAATTCGAC AGTTCGTTGA TTTATGCTCT ATGAGTAATA TATCAGTGTT TCTGTTATCC 
CACAAATGTT TTGGATATTA CATTCATGGT AGATCAGTAC ATGGGCATGC AGATACTAAT 
ATGGAAGAAA TGAATATGAA CCTTAAAAGA GAAGCGGAAA ATTTGTGTAG CCAGAGAGGT 
TTGGTACCCA ACACAGATGG TCAGACTTTT GAGATTGCAA TTTCTAACCA GATGAGACAA 
CATTATGACA GAATTCATGA GACACTAATA AGGAAAAATG GTCCTGCTAG ACTACTGAGT 
TCATCAGCAA GTACTTTTGA GCAGAGTATA AAAGCATATC ATATGATGAA TAAATTTCTT 
GGCTCCTTCA TTGACCATGT TCATAAGGAA ATGGATTACT TTATAAAAGA TAAGTTGCTT 
CTTGAAAGAA TTCTTGGAAT GGAATTCATG GAACCAATGG AAAAAAGCAT CTTTTACAAT 
GATGAAGGTT ATTCTTTCAG CAGTGTCCTG TATTATGGAA ATGAAGCTAC TCTTCTTATT 
TTTGATCTGC TGTTCTTCTG TGTTGTGGAT TTGGCTTGCC AAAATTTTAT TTTAGCATCC 
TTCCTTACAT ATCTACAACA AGAGATTTTT AGATATATCC GTAATACAGT AGGACAAAAG 
AATTTGGCAT CCAAAACATT GGTGGATCAA AGATTTTTGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM67 ( XM_006716686.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006716749.1 Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu42606
Clone ID Related Accession (Same CDS sequence)	XM_006716686.3 , XM_006716686.4
Accession Version	XM_006716686.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	meckelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006716686.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAGA AAGGTGTTAC AGAAGATGGC TGGAACTGCA TTTCTTGCCC TAGTGACTTA 
ACTGCCGAAG GAAAATGTCA CTGTCCCATT GGCCATATTT TAGTGGAAAG AGACATTAAT 
GGAACATTGT TGTCTCAAGC AACTTGTGAG CTCTGTGATG GAAATGAAAA CTCTTTTATG 
GTAGTAAATG CTTTAGGAGA CAGGTGCGTC CGATGTGAGC CAACATTTGT TAATACCAGC 
AGGTCCTGTG CATGTTCAGA ACCTAACATT TTAACAGGGG GATTATGTTT CAGCAGCACA 
GGGAATTTTC CTCTACGTAG AATTTCAGCT GCACGTTATG GAGAAGTTGG CATGTCTTTA 
ACTTCAGAAT GGTTTGCAAA GTATTTGCAA TCATCAGCAG CTGCATGTTG GGTATATGCC 
AATCTAACAT CTTGTCAAGC TCTTGGAAAT ATGTGTGTGA TGAACATGAA TTCTTACGAC 
TTTGCCACAT TTGATGCATG TGGACTATTT CAGTTTATCT TTGAAAATAC TGCTGGACTG 
AGCACTGTTC ATTCTATTTC ATTTTGGAGA CAGAATCTTC CTTGGCTGTT TTATGGAGAC 
CAGTTAGGAT TAGCACCTCA AGTGCTCAGT TCTACCTCTC TTCCTACAAA TTTCAGTTTT 
AAAGGAGAAA ACCAGAATAC AAAACTGAAG TTTGTTGCTG CTTCCTATGA TATAAGAGGA 
AATTTTCTCA AGTGGCAAAC TTTAGAAGGA GGTGTTTTAC AGCTTTGTCC AGACACAGAG 
ACAAGGCTAA ATGCTGCTTA TTCATTTGGA ACAACCTACC AACAAAATTG TGAGATTCCT 
ATCTCTAAGA TCTTAATTGA CTTTCCCACT CCTATATTTT ATGATGTGTA CCTTGAATAT 
ACTGATGAAA ATCAACATCA ATATATTTTG GCTGTGCCTG TGTTAAACCT AAATCTTCAA 
CATAATAAGA TATTTGTGAA CCAAGACAGC AACTCTGGAA AGTGGCTTCT AACTCGGCGC 
ATTTTCTTAG TGGATGCAGT AAGTGGACGA GAAAATGACT TAGGAACTCA GCCAAGAGTA 
ATTCGAGTTG CTACTCAAAT ATCACTGAGT GTCCACCTTG TACCCAACAC AATAAATGGA 
AACATCTACC CTCCCTTAAT CACCATTGCC TACAGTGACA TTGATATCAA AGATGCCAAC 
AGCCAGTCTG TGAAGGTTTC TTTCTCAGTC ACATATGAAA TGGATCATGG AGAAGCACAT 
GTCCAGACAG ATATTGCTTT GGGTGTATTG GGTGGGCTAG CTGTTTTAGC ATCTCTTTTG 
AAGACAGCAG GATGGAAGAG GCGCATTGGG AGTCCCATGA TTGATTTACA GACAGTTGTG 
AAATTCTTGG TGTACTATGC TGGTGATCTG GCCAATGTTT TCTTTATCAT CACAGTGGGA 
ACAGGTCTTT ACTGGCTTAT TTTCTTCAAA GCACAGAAGT CTGTGTCTGT TTTGCTGCCA 
ATGCCAATTC AGGAAGAACG TTTTGTCACT TATGTTGGAT GTGCCTTTGC TCTGAAGGCA 
CTACAATTTT TGCATAAGCT CATATCCCAG ATTACAATAG ATGTATTCTT TATTGATTGG 
GAGCGACCTA AAGGAAAGGT TCTTAAAGCT GTTGAAGGTG AGGGTGGTGT ACGAAGTGCC 
ACTGTTCCTG TAAGCATATG GAGAACATAT TTTGTAGCAA ATGAATGGAA TGAAATTCAG 
ACTGTGAGAA AAATTAATTC ACTCTTTCAA GTACTTACTG TCCTCTTCTT TTTGGAGGTT 
GTGGGATTCA AGAACTTAGC ATTAATGGAC TCATCTTCTA GTCTTTCTAG AAACCCACCT 
AGCTACATAG CTCCTTATAG CTGCATTTTG AGATATGCAG TGTCTGCTGC TCTTTGGCTA 
GCCATTGGAA TTATACAGGT CGTGTTCTTT GCTGTCTTTT ATGAGAGATT TATAGAAGAT 
AAAATTCGAC AGTTCGTTGA TTTATGCTCT ATGAGTAATA TATCAGTGTT TCTGTTATCC 
CACAAATGTT TTGGATATTA CATTCATGGT AGATCAGTAC ATGGGCATGC AGATACTAAT 
ATGGAAGAAA TGAATATGAA CCTTAAAAGA GAAGCGGAAA ATTTGTGTAG CCAGAGAGGT 
TTGGTACCCA ACACAGATGG TCAGACTTTT GAGATTGCAA TTTCTAACCA GATGAGACAA 
CATTATGACA GAATTCATGA GACACTAATA AGGAAAAATG GTCCTGCTAG ACTACTGAGT 
TCATCAGCAA GTACTTTTGA GCAGAGTATA AAAGCATATC ATATGATGAA TAAATTTCTT 
GGCTCCTTCA TTGACCATGT TCATAAGGAA ATGGATTACT TTATAAAAGA TAAGTTGCTT 
CTTGAAAGAA TTCTTGGAAT GGAATTCATG GAACCAATGG AAAAAAGCAT CTTTTACAAT 
GATGAAGGTT ATTCTTTCAG CAGTGTCCTG TATTATGGAA ATGAAGCTAC TCTTCTTATT 
TTTGATCTGC TGTTCTTCTG TGTTGTGGAT TTGGCTTGCC AAAATTTTAT TTTAGCATCC 
TTCCTTACAT ATCTACAACA AGAGATTTTT AGATATATCC GTAATACAGT AGGACAAAAG 
AATTTGGCAT CCAAAACATT GGTGGATCAA AGATTTTTGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006716749.1
CDS	213..2897
Translation	MPEKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTFVNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQTLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTPIFYDVYLEYTDENQHQYILAVPVLNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI

Target ORF information:

RefSeq Version	XM_006716686.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716686.4

ORF Insert Sequence:

ATGCCCGAGA AAGGTGTTAC AGAAGATGGC TGGAACTGCA TTTCTTGCCC TAGTGACTTA 
ACTGCCGAAG GAAAATGTCA CTGTCCCATT GGCCATATTT TAGTGGAAAG AGACATTAAT 
GGAACATTGT TGTCTCAAGC AACTTGTGAG CTCTGTGATG GAAATGAAAA CTCTTTTATG 
GTAGTAAATG CTTTAGGAGA CAGGTGCGTC CGATGTGAGC CAACATTTGT TAATACCAGC 
AGGTCCTGTG CATGTTCAGA ACCTAACATT TTAACAGGGG GATTATGTTT CAGCAGCACA 
GGGAATTTTC CTCTACGTAG AATTTCAGCT GCACGTTATG GAGAAGTTGG CATGTCTTTA 
ACTTCAGAAT GGTTTGCAAA GTATTTGCAA TCATCAGCAG CTGCATGTTG GGTATATGCC 
AATCTAACAT CTTGTCAAGC TCTTGGAAAT ATGTGTGTGA TGAACATGAA TTCTTACGAC 
TTTGCCACAT TTGATGCATG TGGACTATTT CAGTTTATCT TTGAAAATAC TGCTGGACTG 
AGCACTGTTC ATTCTATTTC ATTTTGGAGA CAGAATCTTC CTTGGCTGTT TTATGGAGAC 
CAGTTAGGAT TAGCACCTCA AGTGCTCAGT TCTACCTCTC TTCCTACAAA TTTCAGTTTT 
AAAGGAGAAA ACCAGAATAC AAAACTGAAG TTTGTTGCTG CTTCCTATGA TATAAGAGGA 
AATTTTCTCA AGTGGCAAAC TTTAGAAGGA GGTGTTTTAC AGCTTTGTCC AGACACAGAG 
ACAAGGCTAA ATGCTGCTTA TTCATTTGGA ACAACCTACC AACAAAATTG TGAGATTCCT 
ATCTCTAAGA TCTTAATTGA CTTTCCCACT CCTATATTTT ATGATGTGTA CCTTGAATAT 
ACTGATGAAA ATCAACATCA ATATATTTTG GCTGTGCCTG TGTTAAACCT AAATCTTCAA 
CATAATAAGA TATTTGTGAA CCAAGACAGC AACTCTGGAA AGTGGCTTCT AACTCGGCGC 
ATTTTCTTAG TGGATGCAGT AAGTGGACGA GAAAATGACT TAGGAACTCA GCCAAGAGTA 
ATTCGAGTTG CTACTCAAAT ATCACTGAGT GTCCACCTTG TACCCAACAC AATAAATGGA 
AACATCTACC CTCCCTTAAT CACCATTGCC TACAGTGACA TTGATATCAA AGATGCCAAC 
AGCCAGTCTG TGAAGGTTTC TTTCTCAGTC ACATATGAAA TGGATCATGG AGAAGCACAT 
GTCCAGACAG ATATTGCTTT GGGTGTATTG GGTGGGCTAG CTGTTTTAGC ATCTCTTTTG 
AAGACAGCAG GATGGAAGAG GCGCATTGGG AGTCCCATGA TTGATTTACA GACAGTTGTG 
AAATTCTTGG TGTACTATGC TGGTGATCTG GCCAATGTTT TCTTTATCAT CACAGTGGGA 
ACAGGTCTTT ACTGGCTTAT TTTCTTCAAA GCACAGAAGT CTGTGTCTGT TTTGCTGCCA 
ATGCCAATTC AGGAAGAACG TTTTGTCACT TATGTTGGAT GTGCCTTTGC TCTGAAGGCA 
CTACAATTTT TGCATAAGCT CATATCCCAG ATTACAATAG ATGTATTCTT TATTGATTGG 
GAGCGACCTA AAGGAAAGGT TCTTAAAGCT GTTGAAGGTG AGGGTGGTGT ACGAAGTGCC 
ACTGTTCCTG TAAGCATATG GAGAACATAT TTTGTAGCAA ATGAATGGAA TGAAATTCAG 
ACTGTGAGAA AAATTAATTC ACTCTTTCAA GTACTTACTG TCCTCTTCTT TTTGGAGGTT 
GTGGGATTCA AGAACTTAGC ATTAATGGAC TCATCTTCTA GTCTTTCTAG AAACCCACCT 
AGCTACATAG CTCCTTATAG CTGCATTTTG AGATATGCAG TGTCTGCTGC TCTTTGGCTA 
GCCATTGGAA TTATACAGGT CGTGTTCTTT GCTGTCTTTT ATGAGAGATT TATAGAAGAT 
AAAATTCGAC AGTTCGTTGA TTTATGCTCT ATGAGTAATA TATCAGTGTT TCTGTTATCC 
CACAAATGTT TTGGATATTA CATTCATGGT AGATCAGTAC ATGGGCATGC AGATACTAAT 
ATGGAAGAAA TGAATATGAA CCTTAAAAGA GAAGCGGAAA ATTTGTGTAG CCAGAGAGGT 
TTGGTACCCA ACACAGATGG TCAGACTTTT GAGATTGCAA TTTCTAACCA GATGAGACAA 
CATTATGACA GAATTCATGA GACACTAATA AGGAAAAATG GTCCTGCTAG ACTACTGAGT 
TCATCAGCAA GTACTTTTGA GCAGAGTATA AAAGCATATC ATATGATGAA TAAATTTCTT 
GGCTCCTTCA TTGACCATGT TCATAAGGAA ATGGATTACT TTATAAAAGA TAAGTTGCTT 
CTTGAAAGAA TTCTTGGAAT GGAATTCATG GAACCAATGG AAAAAAGCAT CTTTTACAAT 
GATGAAGGTT ATTCTTTCAG CAGTGTCCTG TATTATGGAA ATGAAGCTAC TCTTCTTATT 
TTTGATCTGC TGTTCTTCTG TGTTGTGGAT TTGGCTTGCC AAAATTTTAT TTTAGCATCC 
TTCCTTACAT ATCTACAACA AGAGATTTTT AGATATATCC GTAATACAGT AGGACAAAAG 
AATTTGGCAT CCAAAACATT GGTGGATCAA AGATTTTTGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM67 ( XM_011517363.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515665.1 Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X7, mRNA.

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CloneID	OHu74308
Clone ID Related Accession (Same CDS sequence)	XM_011517363.3 , XM_011517363.2
Accession Version	XM_011517363.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	meckelin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011517363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC GCGGTGGGGC TGGGGTGGCA ATGGCGGTTT GGTCCCTCTT ATCCGCCCGG 
GCCGTGACCG CGTTCCTTCT GTTGTTCCTC CCTCGCTTCT TACAGGCCCA GACCTTCTCT 
TTCCCTTTCC AGCAGCCGGA GAAGTGCGAC AACAACCAGT ACTTTGATAT CTCCGCCCTC 
TCGTGTGTTC CTTGTGGAGC TAACCAGAGG CAAGATGCCC GAGGAACTTC ATGTGTATGT 
CTACCAGGAT TTCAGATGAT CTCTAATAAT GGAGGACCTG CTATTATTTG TAAAAAGTGC 
CCAGAAAACA TGAAAGGTGT TACAGAAGAT GGCTGGAACT GCATTTCTTG CCCTAGTGAC 
TTAACTGCCG AAGGAAAATG TCACTGTCCC ATTGGCCATA TTTTAGACAG CAACTCTGGA 
AAGTGGCTTC TAACTCGGCG CATTTTCTTA GTGGATGCAG TAAGTGGACG AGAAAATGAC 
TTAGGAACTC AGCCAAGAGT AATTCGAGTT GCTACTCAAA TATCACTGAG TGTCCACCTT 
GTACCCAACA CAATAAATGG AAACATCTAC CCTCCCTTAA TCACCATTGC CTACAGTGAC 
ATTGATATCA AAGATGCCAA CAGCCAGTCT GTGAAGGTTT CTTTCTCAGT CACATATGAA 
ATGGATCATG GAGAAGCACA TGTCCAGACA GATATTGCTT TGGGTGTATT GGGTGGGCTA 
GCTGTTTTAG CATCTCTTTT GAAGACAGCA GGATGGAAGA GGCGCATTGG GAGTCCCATG 
ATTGATTTAC AGACAGTTGT GAAATTCTTG GTGTACTATG CTGGTGATCT GGCCAATGTT 
TTCTTTATCA TCACAGTGGG AACAGGTCTT TACTGGCTTA TTTTCTTCAA AGCACAGAAG 
TCTGTGTCTG TTTTGCTGCC AATGCCAATT CAGGAAGAAC GTTTTGTCAC TTATGTTGGA 
TGTGCCTTTG CTCTGAAGGC ACTACAATTT TTGCATAAGC TCATATCCCA GATTACAATA 
GATGTATTCT TTATTGATTG GGAGCGACCT AAAGGAAAGG TTCTTAAAGC TGTTGAAGGT 
GAGGGTGGTG TACGAAGTGC CACTGTTCCT GTAAGCATAT GGAGAACATA TTTTGTAGCA 
AATGAATGGA ATGAAATTCA GACTGTGAGA AAAATTAATT CACTCTTTCA AGTACTTACT 
GTCCTCTTCT TTTTGGAGGT TGTGGGATTC AAGAACTTAG CATTAATGGA CTCATCTTCT 
AGTCTTTCTA GAAACCCACC TAGCTACATA GCTCCTTATA GCTGCATTTT GAGATATGCA 
GTGTCTGCTG CTCTTTGGCT AGCCATTGGA ATTATACAGG TCGTGTTCTT TGCTGTCTTT 
TATGAGAGAT TTATAGAAGA TAAAATTCGA CAGTTCGTTG ATTTATGCTC TATGAGTAAT 
ATATCAGTGT TTCTGTTATC CCACAAATGT TTTGGATATT ACATTCATGG TAGATCAGTA 
CATGGGCATG CAGATACTAA TATGGAAGAA ATGAATATGA ACCTTAAAAG AGAAGCGGAA 
AATTTGTGTA GCCAGAGAGG TTTGGTACCC AACACAGATG GTCAGACTTT TGAGATTGCA 
ATTTCTAACC AGATGAGACA ACATTATGAC AGAATTCATG AGACACTAAT AAGGAAAAAT 
GGTCCTGCTA GACTACTGAG TTCATCAGCA AGTACTTTTG AGCAGAGTAT AAAAGCATAT 
CATATGATGA ATAAATTTCT TGGCTCCTTC ATTGACCATG TTCATAAGGA AATGGATTAC 
TTTATAAAAG ATAAGTTGCT TCTTGAAAGA ATTCTTGGAA TGGAATTCAT GGAACCAATG 
GAAAAAAGCA TCTTTTACAA TGATGAAGGT TATTCTTTCA GCAGTGTCCT GTATTATGGA 
AATGAAGCTA CTCTTCTTAT TTTTGATCTG CTGTTCTTCT GTGTTGTGGA TTTGGCTTGC 
CAAAATTTTA TTTTAGCATC CTTCCTTACA TATCTACAAC AAGAGATTTT TAGATATATC 
CGTAATACAG TAGGACAAAA GAATTTGGCA TCCAAAACAT TGGTGGATCA AAGATTTTTG 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515665.1
CDS	16..2121
Translation	MATRGGAGVAMAVWSLLSARAVTAFLLLFLPRFLQAQTFSFPFQQPEKCDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI

Target ORF information:

RefSeq Version	XM_011517363.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517363.3

ORF Insert Sequence:

ATGGCGACGC GCGGTGGGGC TGGGGTGGCA ATGGCGGTTT GGTCCCTCTT ATCCGCCCGG 
GCCGTGACCG CGTTCCTTCT GTTGTTCCTC CCTCGCTTCT TACAGGCCCA GACCTTCTCT 
TTCCCTTTCC AGCAGCCGGA GAAGTGCGAC AACAACCAGT ACTTTGATAT CTCCGCCCTC 
TCGTGTGTTC CTTGTGGAGC TAACCAGAGG CAAGATGCCC GAGGAACTTC ATGTGTATGT 
CTACCAGGAT TTCAGATGAT CTCTAATAAT GGAGGACCTG CTATTATTTG TAAAAAGTGC 
CCAGAAAACA TGAAAGGTGT TACAGAAGAT GGCTGGAACT GCATTTCTTG CCCTAGTGAC 
TTAACTGCCG AAGGAAAATG TCACTGTCCC ATTGGCCATA TTTTAGACAG CAACTCTGGA 
AAGTGGCTTC TAACTCGGCG CATTTTCTTA GTGGATGCAG TAAGTGGACG AGAAAATGAC 
TTAGGAACTC AGCCAAGAGT AATTCGAGTT GCTACTCAAA TATCACTGAG TGTCCACCTT 
GTACCCAACA CAATAAATGG AAACATCTAC CCTCCCTTAA TCACCATTGC CTACAGTGAC 
ATTGATATCA AAGATGCCAA CAGCCAGTCT GTGAAGGTTT CTTTCTCAGT CACATATGAA 
ATGGATCATG GAGAAGCACA TGTCCAGACA GATATTGCTT TGGGTGTATT GGGTGGGCTA 
GCTGTTTTAG CATCTCTTTT GAAGACAGCA GGATGGAAGA GGCGCATTGG GAGTCCCATG 
ATTGATTTAC AGACAGTTGT GAAATTCTTG GTGTACTATG CTGGTGATCT GGCCAATGTT 
TTCTTTATCA TCACAGTGGG AACAGGTCTT TACTGGCTTA TTTTCTTCAA AGCACAGAAG 
TCTGTGTCTG TTTTGCTGCC AATGCCAATT CAGGAAGAAC GTTTTGTCAC TTATGTTGGA 
TGTGCCTTTG CTCTGAAGGC ACTACAATTT TTGCATAAGC TCATATCCCA GATTACAATA 
GATGTATTCT TTATTGATTG GGAGCGACCT AAAGGAAAGG TTCTTAAAGC TGTTGAAGGT 
GAGGGTGGTG TACGAAGTGC CACTGTTCCT GTAAGCATAT GGAGAACATA TTTTGTAGCA 
AATGAATGGA ATGAAATTCA GACTGTGAGA AAAATTAATT CACTCTTTCA AGTACTTACT 
GTCCTCTTCT TTTTGGAGGT TGTGGGATTC AAGAACTTAG CATTAATGGA CTCATCTTCT 
AGTCTTTCTA GAAACCCACC TAGCTACATA GCTCCTTATA GCTGCATTTT GAGATATGCA 
GTGTCTGCTG CTCTTTGGCT AGCCATTGGA ATTATACAGG TCGTGTTCTT TGCTGTCTTT 
TATGAGAGAT TTATAGAAGA TAAAATTCGA CAGTTCGTTG ATTTATGCTC TATGAGTAAT 
ATATCAGTGT TTCTGTTATC CCACAAATGT TTTGGATATT ACATTCATGG TAGATCAGTA 
CATGGGCATG CAGATACTAA TATGGAAGAA ATGAATATGA ACCTTAAAAG AGAAGCGGAA 
AATTTGTGTA GCCAGAGAGG TTTGGTACCC AACACAGATG GTCAGACTTT TGAGATTGCA 
ATTTCTAACC AGATGAGACA ACATTATGAC AGAATTCATG AGACACTAAT AAGGAAAAAT 
GGTCCTGCTA GACTACTGAG TTCATCAGCA AGTACTTTTG AGCAGAGTAT AAAAGCATAT 
CATATGATGA ATAAATTTCT TGGCTCCTTC ATTGACCATG TTCATAAGGA AATGGATTAC 
TTTATAAAAG ATAAGTTGCT TCTTGAAAGA ATTCTTGGAA TGGAATTCAT GGAACCAATG 
GAAAAAAGCA TCTTTTACAA TGATGAAGGT TATTCTTTCA GCAGTGTCCT GTATTATGGA 
AATGAAGCTA CTCTTCTTAT TTTTGATCTG CTGTTCTTCT GTGTTGTGGA TTTGGCTTGC 
CAAAATTTTA TTTTAGCATC CTTCCTTACA TATCTACAAC AAGAGATTTT TAGATATATC 
CGTAATACAG TAGGACAAAA GAATTTGGCA TCCAAAACAT TGGTGGATCA AAGATTTTTG 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM67 ( XM_011517363.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515665.1 Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu74308
Clone ID Related Accession (Same CDS sequence)	XM_011517363.3 , XM_011517363.2
Accession Version	XM_011517363.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	meckelin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC GCGGTGGGGC TGGGGTGGCA ATGGCGGTTT GGTCCCTCTT ATCCGCCCGG 
GCCGTGACCG CGTTCCTTCT GTTGTTCCTC CCTCGCTTCT TACAGGCCCA GACCTTCTCT 
TTCCCTTTCC AGCAGCCGGA GAAGTGCGAC AACAACCAGT ACTTTGATAT CTCCGCCCTC 
TCGTGTGTTC CTTGTGGAGC TAACCAGAGG CAAGATGCCC GAGGAACTTC ATGTGTATGT 
CTACCAGGAT TTCAGATGAT CTCTAATAAT GGAGGACCTG CTATTATTTG TAAAAAGTGC 
CCAGAAAACA TGAAAGGTGT TACAGAAGAT GGCTGGAACT GCATTTCTTG CCCTAGTGAC 
TTAACTGCCG AAGGAAAATG TCACTGTCCC ATTGGCCATA TTTTAGACAG CAACTCTGGA 
AAGTGGCTTC TAACTCGGCG CATTTTCTTA GTGGATGCAG TAAGTGGACG AGAAAATGAC 
TTAGGAACTC AGCCAAGAGT AATTCGAGTT GCTACTCAAA TATCACTGAG TGTCCACCTT 
GTACCCAACA CAATAAATGG AAACATCTAC CCTCCCTTAA TCACCATTGC CTACAGTGAC 
ATTGATATCA AAGATGCCAA CAGCCAGTCT GTGAAGGTTT CTTTCTCAGT CACATATGAA 
ATGGATCATG GAGAAGCACA TGTCCAGACA GATATTGCTT TGGGTGTATT GGGTGGGCTA 
GCTGTTTTAG CATCTCTTTT GAAGACAGCA GGATGGAAGA GGCGCATTGG GAGTCCCATG 
ATTGATTTAC AGACAGTTGT GAAATTCTTG GTGTACTATG CTGGTGATCT GGCCAATGTT 
TTCTTTATCA TCACAGTGGG AACAGGTCTT TACTGGCTTA TTTTCTTCAA AGCACAGAAG 
TCTGTGTCTG TTTTGCTGCC AATGCCAATT CAGGAAGAAC GTTTTGTCAC TTATGTTGGA 
TGTGCCTTTG CTCTGAAGGC ACTACAATTT TTGCATAAGC TCATATCCCA GATTACAATA 
GATGTATTCT TTATTGATTG GGAGCGACCT AAAGGAAAGG TTCTTAAAGC TGTTGAAGGT 
GAGGGTGGTG TACGAAGTGC CACTGTTCCT GTAAGCATAT GGAGAACATA TTTTGTAGCA 
AATGAATGGA ATGAAATTCA GACTGTGAGA AAAATTAATT CACTCTTTCA AGTACTTACT 
GTCCTCTTCT TTTTGGAGGT TGTGGGATTC AAGAACTTAG CATTAATGGA CTCATCTTCT 
AGTCTTTCTA GAAACCCACC TAGCTACATA GCTCCTTATA GCTGCATTTT GAGATATGCA 
GTGTCTGCTG CTCTTTGGCT AGCCATTGGA ATTATACAGG TCGTGTTCTT TGCTGTCTTT 
TATGAGAGAT TTATAGAAGA TAAAATTCGA CAGTTCGTTG ATTTATGCTC TATGAGTAAT 
ATATCAGTGT TTCTGTTATC CCACAAATGT TTTGGATATT ACATTCATGG TAGATCAGTA 
CATGGGCATG CAGATACTAA TATGGAAGAA ATGAATATGA ACCTTAAAAG AGAAGCGGAA 
AATTTGTGTA GCCAGAGAGG TTTGGTACCC AACACAGATG GTCAGACTTT TGAGATTGCA 
ATTTCTAACC AGATGAGACA ACATTATGAC AGAATTCATG AGACACTAAT AAGGAAAAAT 
GGTCCTGCTA GACTACTGAG TTCATCAGCA AGTACTTTTG AGCAGAGTAT AAAAGCATAT 
CATATGATGA ATAAATTTCT TGGCTCCTTC ATTGACCATG TTCATAAGGA AATGGATTAC 
TTTATAAAAG ATAAGTTGCT TCTTGAAAGA ATTCTTGGAA TGGAATTCAT GGAACCAATG 
GAAAAAAGCA TCTTTTACAA TGATGAAGGT TATTCTTTCA GCAGTGTCCT GTATTATGGA 
AATGAAGCTA CTCTTCTTAT TTTTGATCTG CTGTTCTTCT GTGTTGTGGA TTTGGCTTGC 
CAAAATTTTA TTTTAGCATC CTTCCTTACA TATCTACAAC AAGAGATTTT TAGATATATC 
CGTAATACAG TAGGACAAAA GAATTTGGCA TCCAAAACAT TGGTGGATCA AAGATTTTTG 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515665.1
CDS	44..2149
Translation	MATRGGAGVAMAVWSLLSARAVTAFLLLFLPRFLQAQTFSFPFQQPEKCDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI

Target ORF information:

RefSeq Version	XM_011517363.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517363.2

ORF Insert Sequence:

ATGGCGACGC GCGGTGGGGC TGGGGTGGCA ATGGCGGTTT GGTCCCTCTT ATCCGCCCGG 
GCCGTGACCG CGTTCCTTCT GTTGTTCCTC CCTCGCTTCT TACAGGCCCA GACCTTCTCT 
TTCCCTTTCC AGCAGCCGGA GAAGTGCGAC AACAACCAGT ACTTTGATAT CTCCGCCCTC 
TCGTGTGTTC CTTGTGGAGC TAACCAGAGG CAAGATGCCC GAGGAACTTC ATGTGTATGT 
CTACCAGGAT TTCAGATGAT CTCTAATAAT GGAGGACCTG CTATTATTTG TAAAAAGTGC 
CCAGAAAACA TGAAAGGTGT TACAGAAGAT GGCTGGAACT GCATTTCTTG CCCTAGTGAC 
TTAACTGCCG AAGGAAAATG TCACTGTCCC ATTGGCCATA TTTTAGACAG CAACTCTGGA 
AAGTGGCTTC TAACTCGGCG CATTTTCTTA GTGGATGCAG TAAGTGGACG AGAAAATGAC 
TTAGGAACTC AGCCAAGAGT AATTCGAGTT GCTACTCAAA TATCACTGAG TGTCCACCTT 
GTACCCAACA CAATAAATGG AAACATCTAC CCTCCCTTAA TCACCATTGC CTACAGTGAC 
ATTGATATCA AAGATGCCAA CAGCCAGTCT GTGAAGGTTT CTTTCTCAGT CACATATGAA 
ATGGATCATG GAGAAGCACA TGTCCAGACA GATATTGCTT TGGGTGTATT GGGTGGGCTA 
GCTGTTTTAG CATCTCTTTT GAAGACAGCA GGATGGAAGA GGCGCATTGG GAGTCCCATG 
ATTGATTTAC AGACAGTTGT GAAATTCTTG GTGTACTATG CTGGTGATCT GGCCAATGTT 
TTCTTTATCA TCACAGTGGG AACAGGTCTT TACTGGCTTA TTTTCTTCAA AGCACAGAAG 
TCTGTGTCTG TTTTGCTGCC AATGCCAATT CAGGAAGAAC GTTTTGTCAC TTATGTTGGA 
TGTGCCTTTG CTCTGAAGGC ACTACAATTT TTGCATAAGC TCATATCCCA GATTACAATA 
GATGTATTCT TTATTGATTG GGAGCGACCT AAAGGAAAGG TTCTTAAAGC TGTTGAAGGT 
GAGGGTGGTG TACGAAGTGC CACTGTTCCT GTAAGCATAT GGAGAACATA TTTTGTAGCA 
AATGAATGGA ATGAAATTCA GACTGTGAGA AAAATTAATT CACTCTTTCA AGTACTTACT 
GTCCTCTTCT TTTTGGAGGT TGTGGGATTC AAGAACTTAG CATTAATGGA CTCATCTTCT 
AGTCTTTCTA GAAACCCACC TAGCTACATA GCTCCTTATA GCTGCATTTT GAGATATGCA 
GTGTCTGCTG CTCTTTGGCT AGCCATTGGA ATTATACAGG TCGTGTTCTT TGCTGTCTTT 
TATGAGAGAT TTATAGAAGA TAAAATTCGA CAGTTCGTTG ATTTATGCTC TATGAGTAAT 
ATATCAGTGT TTCTGTTATC CCACAAATGT TTTGGATATT ACATTCATGG TAGATCAGTA 
CATGGGCATG CAGATACTAA TATGGAAGAA ATGAATATGA ACCTTAAAAG AGAAGCGGAA 
AATTTGTGTA GCCAGAGAGG TTTGGTACCC AACACAGATG GTCAGACTTT TGAGATTGCA 
ATTTCTAACC AGATGAGACA ACATTATGAC AGAATTCATG AGACACTAAT AAGGAAAAAT 
GGTCCTGCTA GACTACTGAG TTCATCAGCA AGTACTTTTG AGCAGAGTAT AAAAGCATAT 
CATATGATGA ATAAATTTCT TGGCTCCTTC ATTGACCATG TTCATAAGGA AATGGATTAC 
TTTATAAAAG ATAAGTTGCT TCTTGAAAGA ATTCTTGGAA TGGAATTCAT GGAACCAATG 
GAAAAAAGCA TCTTTTACAA TGATGAAGGT TATTCTTTCA GCAGTGTCCT GTATTATGGA 
AATGAAGCTA CTCTTCTTAT TTTTGATCTG CTGTTCTTCT GTGTTGTGGA TTTGGCTTGC 
CAAAATTTTA TTTTAGCATC CTTCCTTACA TATCTACAAC AAGAGATTTT TAGATATATC 
CGTAATACAG TAGGACAAAA GAATTTGGCA TCCAAAACAT TGGTGGATCA AAGATTTTTG 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM67 ( NM_153704.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_714915.3 Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26304
Clone ID Related Accession (Same CDS sequence)	NM_153704.6 , NM_153704.5
Accession Version	NM_153704.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	meckelin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK308071.1, BC031220.1, BC054338.1, AC010834.19 and BM310097.1. On Aug 31, 2019 this sequence version replaced NM_153704.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054338.1, BX648768.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGC GCGGTGGGGC TGGGGTGGCA ATGGCGGTTT GGTCCCTCTT ATCCGCCCGG 
GCCGTGACCG CGTTCCTTCT GTTGTTCCTC CCTCGCTTCT TACAGGCCCA GACCTTCTCT 
TTCCCTTTCC AGCAGCCGGA GAAGTGCGAC AACAACCAGT ACTTTGATAT CTCCGCCCTC 
TCGTGTGTTC CTTGTGGAGC TAACCAGAGG CAAGATGCCC GAGGAACTTC ATGTGTATGT 
CTACCAGGAT TTCAGATGAT CTCTAATAAT GGAGGACCTG CTATTATTTG TAAAAAGTGC 
CCAGAAAACA TGAAAGGTGT TACAGAAGAT GGCTGGAACT GCATTTCTTG CCCTAGTGAC 
TTAACTGCCG AAGGAAAATG TCACTGTCCC ATTGGCCATA TTTTAGTGGA AAGAGACATT 
AATGGAACAT TGTTGTCTCA AGCAACTTGT GAGCTCTGTG ATGGAAATGA AAACTCTTTT 
ATGGTAGTAA ATGCTTTAGG AGACAGGTGC GTCCGATGTG AGCCAACATT TGTTAATACC 
AGCAGGTCCT GTGCATGTTC AGAACCTAAC ATTTTAACAG GGGGATTATG TTTCAGCAGC 
ACAGGGAATT TTCCTCTACG TAGAATTTCA GCTGCACGTT ATGGAGAAGT TGGCATGTCT 
TTAACTTCAG AATGGTTTGC AAAGTATTTG CAATCATCAG CAGCTGCATG TTGGGTATAT 
GCCAATCTAA CATCTTGTCA AGCTCTTGGA AATATGTGTG TGATGAACAT GAATTCTTAC 
GACTTTGCCA CATTTGATGC ATGTGGACTA TTTCAGTTTA TCTTTGAAAA TACTGCTGGA 
CTGAGCACTG TTCATTCTAT TTCATTTTGG AGACAGAATC TTCCTTGGCT GTTTTATGGA 
GACCAGTTAG GATTAGCACC TCAAGTGCTC AGTTCTACCT CTCTTCCTAC AAATTTCAGT 
TTTAAAGGAG AAAACCAGAA TACAAAACTG AAGTTTGTTG CTGCTTCCTA TGATATAAGA 
GGAAATTTTC TCAAGTGGCA AACTTTAGAA GGAGGTGTTT TACAGCTTTG TCCAGACACA 
GAGACAAGGC TAAATGCTGC TTATTCATTT GGAACAACCT ACCAACAAAA TTGTGAGATT 
CCTATCTCTA AGATCTTAAT TGACTTTCCC ACTCCTATAT TTTATGATGT GTACCTTGAA 
TATACTGATG AAAATCAACA TCAATATATT TTGGCTGTGC CTGTGTTAAA CCTAAATCTT 
CAACATAATA AGATATTTGT GAACCAAGAC AGCAACTCTG GAAAGTGGCT TCTAACTCGG 
CGCATTTTCT TAGTGGATGC AGTAAGTGGA CGAGAAAATG ACTTAGGAAC TCAGCCAAGA 
GTAATTCGAG TTGCTACTCA AATATCACTG AGTGTCCACC TTGTACCCAA CACAATAAAT 
GGAAACATCT ACCCTCCCTT AATCACCATT GCCTACAGTG ACATTGATAT CAAAGATGCC 
AACAGCCAGT CTGTGAAGGT TTCTTTCTCA GTCACATATG AAATGGATCA TGGAGAAGCA 
CATGTCCAGA CAGATATTGC TTTGGGTGTA TTGGGTGGGC TAGCTGTTTT AGCATCTCTT 
TTGAAGACAG CAGGATGGAA GAGGCGCATT GGGAGTCCCA TGATTGATTT ACAGACAGTT 
GTGAAATTCT TGGTGTACTA TGCTGGTGAT CTGGCCAATG TTTTCTTTAT CATCACAGTG 
GGAACAGGTC TTTACTGGCT TATTTTCTTC AAAGCACAGA AGTCTGTGTC TGTTTTGCTG 
CCAATGCCAA TTCAGGAAGA ACGTTTTGTC ACTTATGTTG GATGTGCCTT TGCTCTGAAG 
GCACTACAAT TTTTGCATAA GCTCATATCC CAGATTACAA TAGATGTATT CTTTATTGAT 
TGGGAGCGAC CTAAAGGAAA GGTTCTTAAA GCTGTTGAAG GTGAGGGTGG TGTACGAAGT 
GCCACTGTTC CTGTAAGCAT ATGGAGAACA TATTTTGTAG CAAATGAATG GAATGAAATT 
CAGACTGTGA GAAAAATTAA TTCACTCTTT CAAGTACTTA CTGTCCTCTT CTTTTTGGAG 
GTTGTGGGAT TCAAGAACTT AGCATTAATG GACTCATCTT CTAGTCTTTC TAGAAACCCA 
CCTAGCTACA TAGCTCCTTA TAGCTGCATT TTGAGATATG CAGTGTCTGC TGCTCTTTGG 
CTAGCCATTG GAATTATACA GGTCGTGTTC TTTGCTGTCT TTTATGAGAG ATTTATAGAA 
GATAAAATTC GACAGTTCGT TGATTTATGC TCTATGAGTA ATATATCAGT GTTTCTGTTA 
TCCCACAAAT GTTTTGGATA TTACATTCAT GGTAGATCAG TACATGGGCA TGCAGATACT 
AATATGGAAG AAATGAATAT GAACCTTAAA AGAGAAGCGG AAAATTTGTG TAGCCAGAGA 
GGTTTGGTAC CCAACACAGA TGGTCAGACT TTTGAGATTG CAATTTCTAA CCAGATGAGA 
CAACATTATG ACAGAATTCA TGAGACACTA ATAAGGAAAA ATGGTCCTGC TAGACTACTG 
AGTTCATCAG CAAGTACTTT TGAGCAGAGT ATAAAAGCAT ATCATATGAT GAATAAATTT 
CTTGGCTCCT TCATTGACCA TGTTCATAAG GAAATGGATT ACTTTATAAA AGATAAGTTG 
CTTCTTGAAA GAATTCTTGG AATGGAATTC ATGGAACCAA TGGAAAAAAG CATCTTTTAC 
AATGATGAAG GTTATTCTTT CAGCAGTGTC CTGTATTATG GAAATGAAGC TACTCTTCTT 
ATTTTTGATC TGCTGTTCTT CTGTGTTGTG GATTTGGCTT GCCAAAATTT TATTTTAGCA 
TCCTTCCTTA CATATCTACA ACAAGAGATT TTTAGATATA TCCGTAATAC AGTAGGACAA 
AAGAATTTGG CATCCAAAAC ATTGGTGGAT CAAAGATTTT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_714915.3
CDS	22..3009
Translation	MATRGGAGVAMAVWSLLSARAVTAFLLLFLPRFLQAQTFSFPFQQPEKCDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTFVNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQTLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTPIFYDVYLEYTDENQHQYILAVPVLNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI

Target ORF information:

RefSeq Version	NM_153704.6
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153704.6

ORF Insert Sequence:

ATGGCGACGC GCGGTGGGGC TGGGGTGGCA ATGGCGGTTT GGTCCCTCTT ATCCGCCCGG 
GCCGTGACCG CGTTCCTTCT GTTGTTCCTC CCTCGCTTCT TACAGGCCCA GACCTTCTCT 
TTCCCTTTCC AGCAGCCGGA GAAGTGCGAC AACAACCAGT ACTTTGATAT CTCCGCCCTC 
TCGTGTGTTC CTTGTGGAGC TAACCAGAGG CAAGATGCCC GAGGAACTTC ATGTGTATGT 
CTACCAGGAT TTCAGATGAT CTCTAATAAT GGAGGACCTG CTATTATTTG TAAAAAGTGC 
CCAGAAAACA TGAAAGGTGT TACAGAAGAT GGCTGGAACT GCATTTCTTG CCCTAGTGAC 
TTAACTGCCG AAGGAAAATG TCACTGTCCC ATTGGCCATA TTTTAGTGGA AAGAGACATT 
AATGGAACAT TGTTGTCTCA AGCAACTTGT GAGCTCTGTG ATGGAAATGA AAACTCTTTT 
ATGGTAGTAA ATGCTTTAGG AGACAGGTGC GTCCGATGTG AGCCAACATT TGTTAATACC 
AGCAGGTCCT GTGCATGTTC AGAACCTAAC ATTTTAACAG GGGGATTATG TTTCAGCAGC 
ACAGGGAATT TTCCTCTACG TAGAATTTCA GCTGCACGTT ATGGAGAAGT TGGCATGTCT 
TTAACTTCAG AATGGTTTGC AAAGTATTTG CAATCATCAG CAGCTGCATG TTGGGTATAT 
GCCAATCTAA CATCTTGTCA AGCTCTTGGA AATATGTGTG TGATGAACAT GAATTCTTAC 
GACTTTGCCA CATTTGATGC ATGTGGACTA TTTCAGTTTA TCTTTGAAAA TACTGCTGGA 
CTGAGCACTG TTCATTCTAT TTCATTTTGG AGACAGAATC TTCCTTGGCT GTTTTATGGA 
GACCAGTTAG GATTAGCACC TCAAGTGCTC AGTTCTACCT CTCTTCCTAC AAATTTCAGT 
TTTAAAGGAG AAAACCAGAA TACAAAACTG AAGTTTGTTG CTGCTTCCTA TGATATAAGA 
GGAAATTTTC TCAAGTGGCA AACTTTAGAA GGAGGTGTTT TACAGCTTTG TCCAGACACA 
GAGACAAGGC TAAATGCTGC TTATTCATTT GGAACAACCT ACCAACAAAA TTGTGAGATT 
CCTATCTCTA AGATCTTAAT TGACTTTCCC ACTCCTATAT TTTATGATGT GTACCTTGAA 
TATACTGATG AAAATCAACA TCAATATATT TTGGCTGTGC CTGTGTTAAA CCTAAATCTT 
CAACATAATA AGATATTTGT GAACCAAGAC AGCAACTCTG GAAAGTGGCT TCTAACTCGG 
CGCATTTTCT TAGTGGATGC AGTAAGTGGA CGAGAAAATG ACTTAGGAAC TCAGCCAAGA 
GTAATTCGAG TTGCTACTCA AATATCACTG AGTGTCCACC TTGTACCCAA CACAATAAAT 
GGAAACATCT ACCCTCCCTT AATCACCATT GCCTACAGTG ACATTGATAT CAAAGATGCC 
AACAGCCAGT CTGTGAAGGT TTCTTTCTCA GTCACATATG AAATGGATCA TGGAGAAGCA 
CATGTCCAGA CAGATATTGC TTTGGGTGTA TTGGGTGGGC TAGCTGTTTT AGCATCTCTT 
TTGAAGACAG CAGGATGGAA GAGGCGCATT GGGAGTCCCA TGATTGATTT ACAGACAGTT 
GTGAAATTCT TGGTGTACTA TGCTGGTGAT CTGGCCAATG TTTTCTTTAT CATCACAGTG 
GGAACAGGTC TTTACTGGCT TATTTTCTTC AAAGCACAGA AGTCTGTGTC TGTTTTGCTG 
CCAATGCCAA TTCAGGAAGA ACGTTTTGTC ACTTATGTTG GATGTGCCTT TGCTCTGAAG 
GCACTACAAT TTTTGCATAA GCTCATATCC CAGATTACAA TAGATGTATT CTTTATTGAT 
TGGGAGCGAC CTAAAGGAAA GGTTCTTAAA GCTGTTGAAG GTGAGGGTGG TGTACGAAGT 
GCCACTGTTC CTGTAAGCAT ATGGAGAACA TATTTTGTAG CAAATGAATG GAATGAAATT 
CAGACTGTGA GAAAAATTAA TTCACTCTTT CAAGTACTTA CTGTCCTCTT CTTTTTGGAG 
GTTGTGGGAT TCAAGAACTT AGCATTAATG GACTCATCTT CTAGTCTTTC TAGAAACCCA 
CCTAGCTACA TAGCTCCTTA TAGCTGCATT TTGAGATATG CAGTGTCTGC TGCTCTTTGG 
CTAGCCATTG GAATTATACA GGTCGTGTTC TTTGCTGTCT TTTATGAGAG ATTTATAGAA 
GATAAAATTC GACAGTTCGT TGATTTATGC TCTATGAGTA ATATATCAGT GTTTCTGTTA 
TCCCACAAAT GTTTTGGATA TTACATTCAT GGTAGATCAG TACATGGGCA TGCAGATACT 
AATATGGAAG AAATGAATAT GAACCTTAAA AGAGAAGCGG AAAATTTGTG TAGCCAGAGA 
GGTTTGGTAC CCAACACAGA TGGTCAGACT TTTGAGATTG CAATTTCTAA CCAGATGAGA 
CAACATTATG ACAGAATTCA TGAGACACTA ATAAGGAAAA ATGGTCCTGC TAGACTACTG 
AGTTCATCAG CAAGTACTTT TGAGCAGAGT ATAAAAGCAT ATCATATGAT GAATAAATTT 
CTTGGCTCCT TCATTGACCA TGTTCATAAG GAAATGGATT ACTTTATAAA AGATAAGTTG 
CTTCTTGAAA GAATTCTTGG AATGGAATTC ATGGAACCAA TGGAAAAAAG CATCTTTTAC 
AATGATGAAG GTTATTCTTT CAGCAGTGTC CTGTATTATG GAAATGAAGC TACTCTTCTT 
ATTTTTGATC TGCTGTTCTT CTGTGTTGTG GATTTGGCTT GCCAAAATTT TATTTTAGCA 
TCCTTCCTTA CATATCTACA ACAAGAGATT TTTAGATATA TCCGTAATAC AGTAGGACAA 
AAGAATTTGG CATCCAAAAC ATTGGTGGAT CAAAGATTTT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM67 ( XM_024447326.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303094.1 Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu106073
Clone ID Related Accession (Same CDS sequence)	XM_024447326.1
Accession Version	XM_024447326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	meckelin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTAA CTTCAGAATG GTTTGCAAAG TATTTGCAAT CATCAGCAGC TGCATGTTGG 
GTATATGCCA ATCTAACATC TTGTCAAGCT CTTGGAAATA TGTGTGTGAT GAACATGAAT 
TCTTACGACT TTGCCACATT TGATGCATGT GGACTATTTC AGTTTATCTT TGAAAATACT 
GCTGGACTGA GCACTGTTCA TTCTATTTCA TTTTGGAGAC AGAATCTTCC TTGGCTGTTT 
TATGGAGACC AGTTAGGATT AGCACCTCAA GTGCTCAGTT CTACCTCTCT TCCTACAAAT 
TTCAGTTTTA AAGGAGAAAA CCAGAATACA AAACTGAAGT TTGTTGCTGC TTCCTATGAT 
ATAAGAGGAA ATTTTCTCAA GTGGCAAACT TTAGAAGGAG GTGTTTTACA GCTTTGTCCA 
GACACAGAGA CAAGGCTAAA TGCTGCTTAT TCATTTGGAA CAACCTACCA ACAAAATTGT 
GAGATTCCTA TCTCTAAGAT CTTAATTGAC TTTCCCACTC CTATATTTTA TGATGTGTAC 
CTTGAATATA CTGATGAAAA TCAACATCAA TATATTTTGG CTGTGCCTGT GTTAAACCTA 
AATCTTCAAC ATAATAAGAT ATTTGTGAAC CAAGACAGCA ACTCTGGAAA GTGGCTTCTA 
ACTCGGCGCA TTTTCTTAGT GGATGCAGTA AGTGGACGAG AAAATGACTT AGGAACTCAG 
CCAAGAGTAA TTCGAGTTGC TACTCAAATA TCACTGAGTG TCCACCTTGT ACCCAACACA 
ATAAATGGAA ACATCTACCC TCCCTTAATC ACCATTGCCT ACAGTGACAT TGATATCAAA 
GATGCCAACA GCCAGTCTGT GAAGGTTTCT TTCTCAGTCA CATATGAAAT GGATCATGGA 
GAAGCACATG TCCAGACAGA TATTGCTTTG GGTGTATTGG GTGGGCTAGC TGTTTTAGCA 
TCTCTTTTGA AGACAGCAGG ATGGAAGAGG CGCATTGGGA GTCCCATGAT TGATTTACAG 
ACAGTTGTGA AATTCTTGGT GTACTATGCT GGTGATCTGG CCAATGTTTT CTTTATCATC 
ACAGTGGGAA CAGGTCTTTA CTGGCTTATT TTCTTCAAAG CACAGAAGTC TGTGTCTGTT 
TTGCTGCCAA TGCCAATTCA GGAAGAACGT TTTGTCACTT ATGTTGGATG TGCCTTTGCT 
CTGAAGGCAC TACAATTTTT GCATAAGCTC ATATCCCAGA TTACAATAGA TGTATTCTTT 
ATTGATTGGG AGCGACCTAA AGGAAAGGTT CTTAAAGCTG TTGAAGGTGA GGGTGGTGTA 
CGAAGTGCCA CTGTTCCTGT AAGCATATGG AGAACATATT TTGTAGCAAA TGAATGGAAT 
GAAATTCAGA CTGTGAGAAA AATTAATTCA CTCTTTCAAG TACTTACTGT CCTCTTCTTT 
TTGGAGGTTG TGGGATTCAA GAACTTAGCA TTAATGGACT CATCTTCTAG TCTTTCTAGA 
AACCCACCTA GCTACATAGC TCCTTATAGC TGCATTTTGA GATATGCAGT GTCTGCTGCT 
CTTTGGCTAG CCATTGGAAT TATACAGGTC GTGTTCTTTG CTGTCTTTTA TGAGAGATTT 
ATAGAAGATA AAATTCGACA GTTCGTTGAT TTATGCTCTA TGAGTAATAT ATCAGTGTTT 
CTGTTATCCC ACAAATGTTT TGGATATTAC ATTCATGGTA GATCAGTACA TGGGCATGCA 
GATACTAATA TGGAAGAAAT GAATATGAAC CTTAAAAGAG AAGCGGAAAA TTTGTGTAGC 
CAGAGAGGTT TGGTACCCAA CACAGATGGT CAGACTTTTG AGATTGCAAT TTCTAACCAG 
ATGAGACAAC ATTATGACAG AATTCATGAG ACACTAATAA GGAAAAATGG TCCTGCTAGA 
CTACTGAGTT CATCAGCAAG TACTTTTGAG CAGAGTATAA AAGCATATCA TATGATGAAT 
AAATTTCTTG GCTCCTTCAT TGACCATGTT CATAAGGAAA TGGATTACTT TATAAAAGAT 
AAGTTGCTTC TTGAAAGAAT TCTTGGAATG GAATTCATGG AACCAATGGA AAAAAGCATC 
TTTTACAATG ATGAAGGTTA TTCTTTCAGC AGTGTCCTGT ATTATGGAAA TGAAGCTACT 
CTTCTTATTT TTGATCTGCT GTTCTTCTGT GTTGTGGATT TGGCTTGCCA AAATTTTATT 
TTAGCATCCT TCCTTACATA TCTACAACAA GAGATTTTTA GATATATCCG TAATACAGTA 
GGACAAAAGA ATTTGGCATC CAAAACATTG GTGGATCAAA GATTTTTGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303094.1
CDS	412..2745
Translation	MSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQTLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTPIFYDVYLEYTDENQHQYILAVPVLNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI

Target ORF information:

RefSeq Version	XM_024447326.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 67 (TMEM67), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447326.1

ORF Insert Sequence:

ATGTCTTTAA CTTCAGAATG GTTTGCAAAG TATTTGCAAT CATCAGCAGC TGCATGTTGG 
GTATATGCCA ATCTAACATC TTGTCAAGCT CTTGGAAATA TGTGTGTGAT GAACATGAAT 
TCTTACGACT TTGCCACATT TGATGCATGT GGACTATTTC AGTTTATCTT TGAAAATACT 
GCTGGACTGA GCACTGTTCA TTCTATTTCA TTTTGGAGAC AGAATCTTCC TTGGCTGTTT 
TATGGAGACC AGTTAGGATT AGCACCTCAA GTGCTCAGTT CTACCTCTCT TCCTACAAAT 
TTCAGTTTTA AAGGAGAAAA CCAGAATACA AAACTGAAGT TTGTTGCTGC TTCCTATGAT 
ATAAGAGGAA ATTTTCTCAA GTGGCAAACT TTAGAAGGAG GTGTTTTACA GCTTTGTCCA 
GACACAGAGA CAAGGCTAAA TGCTGCTTAT TCATTTGGAA CAACCTACCA ACAAAATTGT 
GAGATTCCTA TCTCTAAGAT CTTAATTGAC TTTCCCACTC CTATATTTTA TGATGTGTAC 
CTTGAATATA CTGATGAAAA TCAACATCAA TATATTTTGG CTGTGCCTGT GTTAAACCTA 
AATCTTCAAC ATAATAAGAT ATTTGTGAAC CAAGACAGCA ACTCTGGAAA GTGGCTTCTA 
ACTCGGCGCA TTTTCTTAGT GGATGCAGTA AGTGGACGAG AAAATGACTT AGGAACTCAG 
CCAAGAGTAA TTCGAGTTGC TACTCAAATA TCACTGAGTG TCCACCTTGT ACCCAACACA 
ATAAATGGAA ACATCTACCC TCCCTTAATC ACCATTGCCT ACAGTGACAT TGATATCAAA 
GATGCCAACA GCCAGTCTGT GAAGGTTTCT TTCTCAGTCA CATATGAAAT GGATCATGGA 
GAAGCACATG TCCAGACAGA TATTGCTTTG GGTGTATTGG GTGGGCTAGC TGTTTTAGCA 
TCTCTTTTGA AGACAGCAGG ATGGAAGAGG CGCATTGGGA GTCCCATGAT TGATTTACAG 
ACAGTTGTGA AATTCTTGGT GTACTATGCT GGTGATCTGG CCAATGTTTT CTTTATCATC 
ACAGTGGGAA CAGGTCTTTA CTGGCTTATT TTCTTCAAAG CACAGAAGTC TGTGTCTGTT 
TTGCTGCCAA TGCCAATTCA GGAAGAACGT TTTGTCACTT ATGTTGGATG TGCCTTTGCT 
CTGAAGGCAC TACAATTTTT GCATAAGCTC ATATCCCAGA TTACAATAGA TGTATTCTTT 
ATTGATTGGG AGCGACCTAA AGGAAAGGTT CTTAAAGCTG TTGAAGGTGA GGGTGGTGTA 
CGAAGTGCCA CTGTTCCTGT AAGCATATGG AGAACATATT TTGTAGCAAA TGAATGGAAT 
GAAATTCAGA CTGTGAGAAA AATTAATTCA CTCTTTCAAG TACTTACTGT CCTCTTCTTT 
TTGGAGGTTG TGGGATTCAA GAACTTAGCA TTAATGGACT CATCTTCTAG TCTTTCTAGA 
AACCCACCTA GCTACATAGC TCCTTATAGC TGCATTTTGA GATATGCAGT GTCTGCTGCT 
CTTTGGCTAG CCATTGGAAT TATACAGGTC GTGTTCTTTG CTGTCTTTTA TGAGAGATTT 
ATAGAAGATA AAATTCGACA GTTCGTTGAT TTATGCTCTA TGAGTAATAT ATCAGTGTTT 
CTGTTATCCC ACAAATGTTT TGGATATTAC ATTCATGGTA GATCAGTACA TGGGCATGCA 
GATACTAATA TGGAAGAAAT GAATATGAAC CTTAAAAGAG AAGCGGAAAA TTTGTGTAGC 
CAGAGAGGTT TGGTACCCAA CACAGATGGT CAGACTTTTG AGATTGCAAT TTCTAACCAG 
ATGAGACAAC ATTATGACAG AATTCATGAG ACACTAATAA GGAAAAATGG TCCTGCTAGA 
CTACTGAGTT CATCAGCAAG TACTTTTGAG CAGAGTATAA AAGCATATCA TATGATGAAT 
AAATTTCTTG GCTCCTTCAT TGACCATGTT CATAAGGAAA TGGATTACTT TATAAAAGAT 
AAGTTGCTTC TTGAAAGAAT TCTTGGAATG GAATTCATGG AACCAATGGA AAAAAGCATC 
TTTTACAATG ATGAAGGTTA TTCTTTCAGC AGTGTCCTGT ATTATGGAAA TGAAGCTACT 
CTTCTTATTT TTGATCTGCT GTTCTTCTGT GTTGTGGATT TGGCTTGCCA AAATTTTATT 
TTAGCATCCT TCCTTACATA TCTACAACAA GAGATTTTTA GATATATCCG TAATACAGTA 
GGACAAAAGA ATTTGGCATC CAAAACATTG GTGGATCAAA GATTTTTGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prospective Evaluation of Kidney Disease in Joubert Syndrome.
Clinical journal of the American Society of Nephrology : CJASN12(12)1962-1973(2017 Dec)
Fleming LR,Doherty DA,Parisi MA,Glass IA,Bryant J,Fischer R,Turkbey B,Choyke P,Daryanani K,Vemulapalli M,Mullikin JC,Malicdan MC,Vilboux T,Sayer JA,Gahl WA,Gunay-Aygun M

Functional validation of novel MKS3/TMEM67 mutations in COACH syndrome.
Scientific reports7(1)10222(2017 Aug)
Lee SH,Nam TS,Li W,Kim JH,Yoon W,Choi YD,Kim KH,Cai H,Kim MJ,Kim C,Choy HE,Kim N,Chay KO,Kim MK,Choi SY

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TMEM67 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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