LRRC8A cDNA ORF clone, Bos taurus(cattle)

The following LRRC8A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa24346 NM_001076807.1
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa24346 XM_024998391.1
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa24346 XM_024998390.1
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa24346 XM_024998392.1
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa24346 XM_005213203.4
Latest version!
Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa24346 XM_005213203.3 Bos taurus leucine-rich repeat containing 8 family member A (LRRC8A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa24346
    Clone ID Related Accession (Same CDS sequence) XM_024998390.1 , NM_001076807.1 , XM_024998391.1 , XM_005213203.3 , XM_005213203.4 , XM_024998392.1
    Accession Version NM_001076807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123432.1. On or before Oct 11, 2006 this sequence version replaced XM_581919.2, XM_879782.1. ##Evidence-Data-START## Transcript exon combination :: BC123432.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070275.1
    CDS212..2644
    Translation

    Target ORF information:

    RefSeq Version NM_001076807.1
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001076807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24346
    Clone ID Related Accession (Same CDS sequence) XM_024998390.1 , NM_001076807.1 , XM_024998391.1 , XM_005213203.3 , XM_005213203.4 , XM_024998392.1
    Accession Version XM_024998390.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854158.1
    CDS423..2855
    Translation

    Target ORF information:

    RefSeq Version XM_024998390.1
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998390.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24346
    Clone ID Related Accession (Same CDS sequence) XM_024998390.1 , NM_001076807.1 , XM_024998391.1 , XM_005213203.3 , XM_005213203.4 , XM_024998392.1
    Accession Version XM_024998391.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854159.1
    CDS519..2951
    Translation

    Target ORF information:

    RefSeq Version XM_024998391.1
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998391.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24346
    Clone ID Related Accession (Same CDS sequence) XM_024998390.1 , NM_001076807.1 , XM_024998391.1 , XM_005213203.3 , XM_005213203.4 , XM_024998392.1
    Accession Version XM_005213203.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213203.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213260.1
    CDS487..2919
    Translation

    Target ORF information:

    RefSeq Version XM_005213203.3
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine-rich repeat containing 8 family member A (LRRC8A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213203.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24346
    Clone ID Related Accession (Same CDS sequence) XM_024998390.1 , NM_001076807.1 , XM_024998391.1 , XM_005213203.3 , XM_005213203.4 , XM_024998392.1
    Accession Version XM_005213203.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213203.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213260.1
    CDS448..2880
    Translation

    Target ORF information:

    RefSeq Version XM_005213203.4
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213203.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24346
    Clone ID Related Accession (Same CDS sequence) XM_024998390.1 , NM_001076807.1 , XM_024998391.1 , XM_005213203.3 , XM_005213203.4 , XM_024998392.1
    Accession Version XM_024998392.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product volume-regulated anion channel subunit LRRC8A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854160.1
    CDS321..2753
    Translation

    Target ORF information:

    RefSeq Version XM_024998392.1
    Organism Bos taurus(cattle)
    Definition Bos taurus leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTCCGG TGACAGAGCT CCGCTACTTT GCGGACACGC AGCCAGCGTA CCGGATCCTG 
    AAGCCGTGGT GGGACGTGTT CACCGACTAC ATCTCCATCG TCATGCTGAT GATTGCTGTC
    TTCGGGGGCA CGCTGCAGGT TACCCAGGAC AAGATGATCT GCCTGCCCTG TAAGTGGGTC
    ACCAAGGACT CCTGCAACGA CTCGTTCCGG GGCTGGGCGG CCCCTGGCCC AGAGCCCCCC
    TACCCCAACT CCACCATCGG GCCGACCCCC GACTCCGGCC CCACCGGCAT CAAGTACGAC
    CTGGACCGGC ACCAGTACAA CTATGTGGAC GCCGTGTGCT ATGAGAACCG CCTGCACTGG
    TTTGCCAAGT ACTTCCCCTA CCTGGTGCTT CTGCACACGC TCATCTTCCT GGCCTGCAGC
    AACTTCTGGT TCAAGTTCCC GCGCACCAGC TCCAAGCTGG AGCACTTCGT GTCCATCCTG
    CTCAAGTGCT TCGACTCGCC CTGGACCACG CGGGCCCTGT CGGAGACGGT GGTGGAGGAG
    AGCGACCCCA AGCCGGCCTT CAGCAAGATG AACGGCTCCA TGGACAAGAA GTCATCGACG
    GTCAGCGAGG ACGTGGAGGC CACCGTGCCC ATGCTGCAGC GGACCAAGTC TCGGATCGAG
    CAGGGCATCG TGGACCGCTC GGAGACGGGC GTGCTGGACA AGAAGGAGGG CGAGCAGGCC
    AAGGCGCTGT TCGAGAAGGT GAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG
    TACCGGCTGT ACATGCGGCA GACCATCATC AAGGTGATCA AGTTCATCCT CATCATCTGC
    TACACCGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGCACGGT GGACATCGAG
    AGCCTGACGG GCTACCGCAC ATACCGGTGC GCCCACCCGC TGGCCACGCT CTTCAAGATC
    CTGGCATCCT TCTACATCAG CCTGGTCATC TTCTACGGCC TCATCTGCAT GTACACGCTC
    TGGTGGATGC TGCGGCGCTC CCTGAAGAAG TACTCGTTCG AGTCCATCCG CGAGGAGAGC
    AGCTACAGCG ACATCCCCGA CGTCAAGAAC GACTTCGCCT TCATGCTGCA CCTCATCGAC
    CAGTACGACC CGCTGTACTC AAAACGCTTC GCTGTCTTCC TGTCGGAGGT GAGTGAGAAC
    AAGCTGCGGC AGCTAAACCT CAACAACGAG TGGACGTTGG ACAAGCTGCG CCAGCGGCTC
    ACCAAGAATG CGCAGGACAA GCTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC
    ACGGTGTTCG ACCTGGTGGA GCTGGAGGTG CTGAAGCTGG AGCTGATCCC CGACGTGACC
    ATCCCGCCCA GCATCGCACA GCTCACGGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCC
    GCCAAGATCG AGGCGCCCGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC GCTGCACATC
    AAGTTCACGG ACATCAAGGA GATCCCGCTG TGGATCTACA GCCTGAAGAC GCTGGAGGAG
    CTGCACCTGA CGGGCAACCT GAGCGCCGAG AACAACCGCT TCATCGTCAT CGACGGGCTC
    CGTGAGCTCA AGCGGCTCAA GGTGCTGAGG CTGAAGAGCA ATCTGAGCAA GCTGCCGCAG
    GTGGTCACGG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACGAAG
    CTCATTGTGC TCAACAGCCT CAAGAAGATG GCCAACCTGA CAGAGCTGGA GCTCATCCGC
    TGCGACCTGG AGCGCATCCC CCACTCCATC TTCAGCCTCC ACAACCTGCA GGAGATCGAC
    CTCAAGGACA ACAACCTCAA GACCATTGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC
    CTCACCTGCC TTAAGCTGTG GTACAACCAT ATCGCCTACA TCCCCATCCA GATCGGCAAC
    CTCACCAACC TCGAGCGCCT CTACCTGAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG
    CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAACCT GACCTTCCTC
    CCCGCTGACA TCGGCCTCCT GCAGAGCCTC CAGAACTTGG CCGTCACGGC CAACCGGATC
    GAGGCGCTGC CACCCGAGCT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC
    AACGTGCTGC AGTCGCTGCC CTCGCGGGTG GGCGAGCTGA CCAGCCTGAC CCAGATCGAG
    CTGCGTGGCA ACCGACTGGA GTGCCTGCCT GTGGAGCTGG GCGAGTGCCC GCTGCTCAAG
    CGCAGTGGCC TGGTGGTGGA GGAGGACCTG TTCAACACCC TGCCCCCTGA GGTGAAGGAG
    CGGCTCTGGA GGGCTGACAA GGAGCAGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.