IGFBP5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	IGFBP5
Entrez Gene ID	404185
Full Name	insulin like growth factor binding protein 5
General protein information	Preferred Names insulin like growth factor binding protein 5 Names insulin-like growth factor-binding protein 5 IBP-5 IGF-binding protein 5 IGFBP-5
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010802317.2	XP_010800619.1	insulin-like growth factor-binding protein 5 isoform X1
NM_001105327.2	NP_001098797.1	insulin-like growth factor-binding protein 5 precursor
XM_010802317.1	XP_010800619.1	insulin-like growth factor-binding protein 5 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	IGFBP5	NP_001098797.1
Mus musculus (house mouse)	Igfbp5	NP_034648.2
Xenopus tropicalis (tropical clawed frog)	igfbp5	NP_001016042.1
Canis lupus familiaris (dog)	IGFBP5	XP_852885.1
Gallus gallus (chicken)	IGFBP5	XP_422069.3
	IGFBP5	XP_001087664.1
Danio rerio (zebrafish)	igfbp5b	NP_001092224.1
Homo sapiens (human)	IGFBP5	NP_000590.1
Pan troglodytes (chimpanzee)	IGFBP5	XP_003309513.1
Rattus norvegicus (Norway rat)	Igfbp5	NP_036949.1
Danio rerio (zebrafish)	igfbp5a	NP_001119935.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-143 regulates proliferation and differentiation of bovine skeletal muscle satellite cells by targeting IGFBP5.
Zhang WR, Zhang HN, Wang YM, Dai Y, Liu XF, Li X, Ding XB, Guo H
In vitro cellular & developmental biology. Animal53(3)265-271(2017 Mar)

Comparative expression profiling of insulin-like growth factor binding protein-5 in milk of Bos indicus and Bubalus bubalis during lactation.
Mohapatra SK, Singh S, Kumar S, Dang AK, Datta TK, Das SK, Mohanty TK, Kaushik JK, Mohanty AK
Animal : an international journal of animal bioscience9(4)643-9(2015 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Growth hormone suppresses the expression of IGFBP-5, and promotes the IGF-I-induced phosphorylation of Akt in bovine mammary epithelial cells.
Sakamoto K, Yano T, Kobayashi T, Hagino A, Aso H, Obara Y
Domestic animal endocrinology32(4)260-72(2007 May)

Alterations in follicular IGFBP mRNA expression and follicular fluid IGFBP concentrations during the first follicle wave in beef heifers.
Canty MJ, Boland MP, Evans AC, Crowe MA
Animal reproduction science93(3-4)199-217(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IGFBP5, an important component of IGF signaling pathway, contributes greatly to bovine muscle cell development. A mechanism that miR-143 can regulate the proliferation and differentiation of bovine MSCs through changing expression of IGFBP5 was elucidated by our study.
Title: miR-143 regulates proliferation and differentiation of bovine skeletal muscle satellite cells by targeting IGFBP5.

This study suggested that that higher level IGFBP-5 expression may have functional significance in lactation persistency.
Title: Comparative expression profiling of insulin-like growth factor binding protein-5 in milk of Bos indicus and Bubalus bubalis during lactation.

GH reduces mRNA and protein expression of IGFBP-5 in bovine mammary epithelial cells, but it does not affect the expression of IGFBP-3
Title: Growth hormone suppresses the expression of IGFBP-5, and promotes the IGF-I-induced phosphorylation of Akt in bovine mammary epithelial cells.

follicular dominance was associated with low or decreased follicular fluid concentrations of IGFBP-4 and -5
Title: Alterations in follicular IGFBP mRNA expression and follicular fluid IGFBP concentrations during the first follicle wave in beef heifers.

The following IGFBP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IGFBP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00738	NM_001105327.2 Latest version!	Bos taurus insulin like growth factor binding protein 5 (IGFBP5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa45655	XM_010802317.2 Latest version!	Bos taurus insulin like growth factor binding protein 5 (IGFBP5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OBa45655	XM_010802317.1	Bos taurus insulin like growth factor binding protein 5 (IGFBP5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IGFBP5 ( NM_001105327.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001098797.1 Bos taurus insulin like growth factor binding protein 5 (IGFBP5), mRNA.

Price & Availability↑

CloneID	OBa00738
Clone ID Related Accession (Same CDS sequence)	NM_001105327.2
Accession Version	NM_001105327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	insulin-like growth factor-binding protein 5 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149394.1. On Aug 31, 2012 this sequence version replaced NM_001105327.1. ##Evidence-Data-START## Transcript exon combination :: BC149394.1, DN511652.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145425, SAMN03145448 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCTCA CCGCGGTCCT CCTTCTTCTG GCCGCCTGTG CGGGATCGGC CCAGGGTCTG 
GGCTCCTTCG TGCACTGCGA GCCCTGCGAC GAGAAAGCCC TCTCCATGTG CCCCCCCAGC 
CCCCTGGGCT GCGAGCTGGT CAAGGAGCCG GGCTGCGGCT GCTGCATGAC CTGCGCCCTG 
GCCGAGGGGC AGTCGTGCGG CGTCTACACT GAGCGCTGCG CCCAGGGGCT GCGTTGCCTC 
CCCCGGCAGG ACGAGGAGAA GCCGCTGCAC GCCCTGCTGC ACGGCCGCGG GGTTTGCCTG 
AACGAAAAGA GCTACCGCGA GCAAGCCAAG ATCGAAAGAG ACTCCCGTGA GCACGAGGAG 
CCCACCACCT CCGAGATGGC AGAGGAGACC TACTCGCCCA AGATCTTCCG GCCCAAGCAC 
ACCCGCATCT CCGAGCTGAA GGCCGAGGCT GTGAAGAAGG ACCGCAGAAA GAAGCTGACC 
CAGTCCAAGT TCGTGGGAGG AGCTGAGAAC ACTGCCCATC CCCGGGTCAT CTCCGCGCCT 
GAGATGAGAC AGAAATCTGA GCAGGGGCCC TGCCGCAGGC ACATGGAGGC GTCCCTGCAG 
GAGCTCAAAG CCAGCCCGCG CATGGTGCCC CGCGCCGTGT ACCTGCCCAA CTGTGACCGC 
AAAGGGTTCT ACAAGAGAAA GCAGTGCAAA CCTTCCCGTG GCCGCAAGCG TGGCATCTGC 
TGGTGCGTGG ACAAGTACGG GATGAAGCTG CCGGGCATGG AGTACGTGGA CGGGGACTTT 
CAGTGCCACA CCTTCGACAG CAGCAACGTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001098797.1
CDS	31..846
Translation	MVLTAVLLLLAACAGSAQGLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQAKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQKSEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Target ORF information:

RefSeq Version	NM_001105327.2
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor binding protein 5 (IGFBP5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105327.2

ORF Insert Sequence:

ATGGTGCTCA CCGCGGTCCT CCTTCTTCTG GCCGCCTGTG CGGGATCGGC CCAGGGTCTG 
GGCTCCTTCG TGCACTGCGA GCCCTGCGAC GAGAAAGCCC TCTCCATGTG CCCCCCCAGC 
CCCCTGGGCT GCGAGCTGGT CAAGGAGCCG GGCTGCGGCT GCTGCATGAC CTGCGCCCTG 
GCCGAGGGGC AGTCGTGCGG CGTCTACACT GAGCGCTGCG CCCAGGGGCT GCGTTGCCTC 
CCCCGGCAGG ACGAGGAGAA GCCGCTGCAC GCCCTGCTGC ACGGCCGCGG GGTTTGCCTG 
AACGAAAAGA GCTACCGCGA GCAAGCCAAG ATCGAAAGAG ACTCCCGTGA GCACGAGGAG 
CCCACCACCT CCGAGATGGC AGAGGAGACC TACTCGCCCA AGATCTTCCG GCCCAAGCAC 
ACCCGCATCT CCGAGCTGAA GGCCGAGGCT GTGAAGAAGG ACCGCAGAAA GAAGCTGACC 
CAGTCCAAGT TCGTGGGAGG AGCTGAGAAC ACTGCCCATC CCCGGGTCAT CTCCGCGCCT 
GAGATGAGAC AGAAATCTGA GCAGGGGCCC TGCCGCAGGC ACATGGAGGC GTCCCTGCAG 
GAGCTCAAAG CCAGCCCGCG CATGGTGCCC CGCGCCGTGT ACCTGCCCAA CTGTGACCGC 
AAAGGGTTCT ACAAGAGAAA GCAGTGCAAA CCTTCCCGTG GCCGCAAGCG TGGCATCTGC 
TGGTGCGTGG ACAAGTACGG GATGAAGCTG CCGGGCATGG AGTACGTGGA CGGGGACTTT 
CAGTGCCACA CCTTCGACAG CAGCAACGTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGFBP5 ( XM_010802317.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800619.1 Bos taurus insulin like growth factor binding protein 5 (IGFBP5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45655
Clone ID Related Accession (Same CDS sequence)	XM_010802317.2 , XM_010802317.1
Accession Version	XM_010802317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	insulin-like growth factor-binding protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA CCGCGGTCCT CCTTCTTCTG GCCGCCTGTG CGGGATCGGC CCAGGGTCTG 
GGCTCCTTCG TGCACTGCGA GCCCTGCGAC GAGAAAGCCC TCTCCATGTG CCCCCCCAGC 
CCCCTGGGCT GCGAGCTGGT CAAGGAGCCG GGCTGCGGCT GCTGCATGAC CTGCGCCCTG 
GCCGAGGGGC AGTCGTGCGG CGTCTACACT GAGCGCTGCG CCCAGGGGCT GCGTTGCCTC 
CCCCGGCAGG ACGAGGAGAA GCCGCTGCAC GCCCTGCTGC ACGGCCGCGG GGTTTGCCTG 
AACGAAAAGA GCTACCGCGA GCAAGCCAAG ATCGAAAGAG ACTCCCGTGA GCACGAGGAG 
CCCACCACCT CCGAGATGGC AGAGGAGACC TACTCGCCCA AGATCTTCCG GCCCAAGCAC 
ACCCGCATCT CCGAGCTGAA GGCCGAGGCT GTGAAGAAGG ACCGCAGAAA GAAGCTGACC 
CAGTCCAAGT TCGTGGGAGG AGCTGAGAAC ACTGCCCATC CCCGGGTCAT CTCCGCGCCT 
GAGATGAGAC AGAAATCTGA GCAGGTGACT CCCTGGGGTC TGGCCACGCC TCTCACGCCT 
CCGATCCGCA CACAGGGGCC CTGCCGCAGG CACATGGAGG CGTCCCTGCA GGAGCTCAAA 
GCCAGCCCGC GCATGGTGCC CCGCGCCGTG TACCTGCCCA ACTGTGACCG CAAAGGGTTC 
TACAAGAGAA AGCAGTGCAA ACCTTCCCGT GGCCGCAAGC GTGGCATCTG CTGGTGCGTG 
GACAAGTACG GGATGAAGCT GCCGGGCATG GAGTACGTGG ACGGGGACTT TCAGTGCCAC 
ACCTTCGACA GCAGCAACGT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800619.1
CDS	977..1843
Translation	MVLTAVLLLLAACAGSAQGLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQAKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQKSEQVTPWGLATPLTPPIRTQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Target ORF information:

RefSeq Version	XM_010802317.2
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor binding protein 5 (IGFBP5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802317.2

ORF Insert Sequence:

ATGGTGCTCA CCGCGGTCCT CCTTCTTCTG GCCGCCTGTG CGGGATCGGC CCAGGGTCTG 
GGCTCCTTCG TGCACTGCGA GCCCTGCGAC GAGAAAGCCC TCTCCATGTG CCCCCCCAGC 
CCCCTGGGCT GCGAGCTGGT CAAGGAGCCG GGCTGCGGCT GCTGCATGAC CTGCGCCCTG 
GCCGAGGGGC AGTCGTGCGG CGTCTACACT GAGCGCTGCG CCCAGGGGCT GCGTTGCCTC 
CCCCGGCAGG ACGAGGAGAA GCCGCTGCAC GCCCTGCTGC ACGGCCGCGG GGTTTGCCTG 
AACGAAAAGA GCTACCGCGA GCAAGCCAAG ATCGAAAGAG ACTCCCGTGA GCACGAGGAG 
CCCACCACCT CCGAGATGGC AGAGGAGACC TACTCGCCCA AGATCTTCCG GCCCAAGCAC 
ACCCGCATCT CCGAGCTGAA GGCCGAGGCT GTGAAGAAGG ACCGCAGAAA GAAGCTGACC 
CAGTCCAAGT TCGTGGGAGG AGCTGAGAAC ACTGCCCATC CCCGGGTCAT CTCCGCGCCT 
GAGATGAGAC AGAAATCTGA GCAGGTGACT CCCTGGGGTC TGGCCACGCC TCTCACGCCT 
CCGATCCGCA CACAGGGGCC CTGCCGCAGG CACATGGAGG CGTCCCTGCA GGAGCTCAAA 
GCCAGCCCGC GCATGGTGCC CCGCGCCGTG TACCTGCCCA ACTGTGACCG CAAAGGGTTC 
TACAAGAGAA AGCAGTGCAA ACCTTCCCGT GGCCGCAAGC GTGGCATCTG CTGGTGCGTG 
GACAAGTACG GGATGAAGCT GCCGGGCATG GAGTACGTGG ACGGGGACTT TCAGTGCCAC 
ACCTTCGACA GCAGCAACGT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGFBP5 ( XM_010802317.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800619.1 Bos taurus insulin like growth factor binding protein 5 (IGFBP5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45655
Clone ID Related Accession (Same CDS sequence)	XM_010802317.2 , XM_010802317.1
Accession Version	XM_010802317.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	insulin-like growth factor-binding protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA CCGCGGTCCT CCTTCTTCTG GCCGCCTGTG CGGGATCGGC CCAGGGTCTG 
GGCTCCTTCG TGCACTGCGA GCCCTGCGAC GAGAAAGCCC TCTCCATGTG CCCCCCCAGC 
CCCCTGGGCT GCGAGCTGGT CAAGGAGCCG GGCTGCGGCT GCTGCATGAC CTGCGCCCTG 
GCCGAGGGGC AGTCGTGCGG CGTCTACACT GAGCGCTGCG CCCAGGGGCT GCGTTGCCTC 
CCCCGGCAGG ACGAGGAGAA GCCGCTGCAC GCCCTGCTGC ACGGCCGCGG GGTTTGCCTG 
AACGAAAAGA GCTACCGCGA GCAAGCCAAG ATCGAAAGAG ACTCCCGTGA GCACGAGGAG 
CCCACCACCT CCGAGATGGC AGAGGAGACC TACTCGCCCA AGATCTTCCG GCCCAAGCAC 
ACCCGCATCT CCGAGCTGAA GGCCGAGGCT GTGAAGAAGG ACCGCAGAAA GAAGCTGACC 
CAGTCCAAGT TCGTGGGAGG AGCTGAGAAC ACTGCCCATC CCCGGGTCAT CTCCGCGCCT 
GAGATGAGAC AGAAATCTGA GCAGGTGACT CCCTGGGGTC TGGCCACGCC TCTCACGCCT 
CCGATCCGCA CACAGGGGCC CTGCCGCAGG CACATGGAGG CGTCCCTGCA GGAGCTCAAA 
GCCAGCCCGC GCATGGTGCC CCGCGCCGTG TACCTGCCCA ACTGTGACCG CAAAGGGTTC 
TACAAGAGAA AGCAGTGCAA ACCTTCCCGT GGCCGCAAGC GTGGCATCTG CTGGTGCGTG 
GACAAGTACG GGATGAAGCT GCCGGGCATG GAGTACGTGG ACGGGGACTT TCAGTGCCAC 
ACCTTCGACA GCAGCAACGT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800619.1
CDS	217..1083
Translation	MVLTAVLLLLAACAGSAQGLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQAKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQKSEQVTPWGLATPLTPPIRTQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Target ORF information:

RefSeq Version	XM_010802317.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor binding protein 5 (IGFBP5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802317.1

ORF Insert Sequence:

ATGGTGCTCA CCGCGGTCCT CCTTCTTCTG GCCGCCTGTG CGGGATCGGC CCAGGGTCTG 
GGCTCCTTCG TGCACTGCGA GCCCTGCGAC GAGAAAGCCC TCTCCATGTG CCCCCCCAGC 
CCCCTGGGCT GCGAGCTGGT CAAGGAGCCG GGCTGCGGCT GCTGCATGAC CTGCGCCCTG 
GCCGAGGGGC AGTCGTGCGG CGTCTACACT GAGCGCTGCG CCCAGGGGCT GCGTTGCCTC 
CCCCGGCAGG ACGAGGAGAA GCCGCTGCAC GCCCTGCTGC ACGGCCGCGG GGTTTGCCTG 
AACGAAAAGA GCTACCGCGA GCAAGCCAAG ATCGAAAGAG ACTCCCGTGA GCACGAGGAG 
CCCACCACCT CCGAGATGGC AGAGGAGACC TACTCGCCCA AGATCTTCCG GCCCAAGCAC 
ACCCGCATCT CCGAGCTGAA GGCCGAGGCT GTGAAGAAGG ACCGCAGAAA GAAGCTGACC 
CAGTCCAAGT TCGTGGGAGG AGCTGAGAAC ACTGCCCATC CCCGGGTCAT CTCCGCGCCT 
GAGATGAGAC AGAAATCTGA GCAGGTGACT CCCTGGGGTC TGGCCACGCC TCTCACGCCT 
CCGATCCGCA CACAGGGGCC CTGCCGCAGG CACATGGAGG CGTCCCTGCA GGAGCTCAAA 
GCCAGCCCGC GCATGGTGCC CCGCGCCGTG TACCTGCCCA ACTGTGACCG CAAAGGGTTC 
TACAAGAGAA AGCAGTGCAA ACCTTCCCGT GGCCGCAAGC GTGGCATCTG CTGGTGCGTG 
GACAAGTACG GGATGAAGCT GCCGGGCATG GAGTACGTGG ACGGGGACTT TCAGTGCCAC 
ACCTTCGACA GCAGCAACGT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-143 regulates proliferation and differentiation of bovine skeletal muscle satellite cells by targeting IGFBP5.
In vitro cellular & developmental biology. Animal53(3)265-271(2017 Mar)
Zhang WR,Zhang HN,Wang YM,Dai Y,Liu XF,Li X,Ding XB,Guo H

Comparative expression profiling of insulin-like growth factor binding protein-5 in milk of Bos indicus and Bubalus bubalis during lactation.
Animal : an international journal of animal bioscience9(4)643-9(2015 Apr)
Mohapatra SK,Singh S,Kumar S,Dang AK,Datta TK,Das SK,Mohanty TK,Kaushik JK,Mohanty AK

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Growth hormone suppresses the expression of IGFBP-5, and promotes the IGF-I-induced phosphorylation of Akt in bovine mammary epithelial cells.
Domestic animal endocrinology32(4)260-72(2007 May)
Sakamoto K,Yano T,Kobayashi T,Hagino A,Aso H,Obara Y

Alterations in follicular IGFBP mRNA expression and follicular fluid IGFBP concentrations during the first follicle wave in beef heifers.
Animal reproduction science93(3-4)199-217(2006 Jul)
Canty MJ,Boland MP,Evans AC,Crowe MA

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IGFBP5 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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