CACNA2D1 cDNA ORF clone, Bos taurus(cattle)

The following CACNA2D1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA2D1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239200 NM_001205987.2
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233539 XM_024990476.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233540 XM_024990477.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233541 XM_024990478.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233542 XM_024990479.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233543 XM_024990480.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233544 XM_024990481.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233545 XM_024990482.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233546 XM_024990483.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233547 XM_024990484.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233548 XM_024990485.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233549 XM_024990486.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa233550 XM_024990487.1
Latest version!
Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa38532 NM_001205987.1
Latest version!
Bos taurus calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa38532 XM_010804141.2
Latest version!
Bos taurus calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239200
    Clone ID Related Accession (Same CDS sequence) NM_001205987.2
    Accession Version NM_001205987.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-08-04
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000004.1. On May 29, 2018 this sequence version replaced NM_001205987.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAACG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG AGGACTATAC CGACTGTGGT
    GGTGTCTCTG GATTAAACCC CTCCCTGTGG TCCATCATCG GCATCCAGTG TGTGCTACTT
    TGGCTCTTAT CTGGCAGCAG ACACCACCAG TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192916.1
    CDS22..3297
    Translation

    Target ORF information:

    RefSeq Version NM_001205987.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205987.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAACG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG AGGACTATAC CGACTGTGGT
    GGTGTCTCTG GATTAAACCC CTCCCTGTGG TCCATCATCG GCATCCAGTG TGTGCTACTT
    TGGCTCTTAT CTGGCAGCAG ACACCACCAG TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233539
    Clone ID Related Accession (Same CDS sequence) XM_024990476.1
    Accession Version XM_024990476.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGAAC CCCAAATCTC AGGAGCCAGT AACTTTGGAT
    TTCCTTGATG CAGAATTAGA GAACGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAT
    GGAGAAAGCG GAGAAAAAAC ATTCAGGACT CTGGTTAAAT CTCAAGATGA GAGATATATT
    GACAAAGGAA ACAGGACATA TACATGGACT CCTGTCAATG GCACAGATTA CAGTTTGGCC
    TTGGTATTAC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT
    CAGGCCAGAT CCAAAAAGGG CAGAATGAAG GATTCAGAAA CTCTGAAACC GGATAATTTT
    GAAGAGTCTG GCTATACGTT CATAGCACCA AGAGATTACT GCAATGACCT TAAAATATCA
    GATAATAACA CCGAATTTCT TTTAAATTTT AATGAGTTTA TTGATAGAAA AACTCCAAAC
    AACCCATCAT GCAACACAGA CTTGATTAAT AGAGTCTTGC TGGATGCAGG CTTTACAAAT
    GAACTTGTCC AAAATTACTG GAGTAAGCAG AAAAACATCA AGGGAGTGAA AGCACGATTT
    GTTGTGACTG ATGGTGGGAT TACCAGAGTT TATCCGAAAG AGGCTGGAGA AAATTGGCAA
    GAAAACCCAG AAACATATGA GGACAGCTTC TATAAAAGAA GTCTAGATAA CGATAACTAT
    GTTTTCACTG CTCCCTACTT TAACAAAAGT GGACCTGGTG CTTATGAATC AGGCATTATG
    GTAAGCAAAG CTGTAGAAAT TTACATCCAA GGAAAACTTC TTAAACCTGC AGTTGTTGGA
    ATTAAAATTG ATCTAAATTC CTGGATAGAG AATTTCACCA AAACATCAAT CAGGGATCCG
    TGTGCGGGTC CAGTTTGTGA CTGTAAAAGA AACAGTGATG TAATGGACTG TGTTATTCTA
    GATGATGGTG GGTTCCTTTT GATGGCAAAT CATGATGATT ATACTAACCA GATTGGAAGA
    TTTTTTGGAG AGATTGATCC AAGTTTGATG AGACACCTGG TTAATATATC AGTTTATGCT
    TATAACAAGT CTTATGATTA TCAGTCGGTG TGTGAGCCAG GAGCTGCACC AAAACAAGGA
    GCAGGGCATC GCTCAGCATA CGTGCCATCA ATAGCCGACG TATTACACAT TGGCTGGTGG
    GCCACTGCTG CTGCCTGGTC TATTCTACAG CAGTTTCTCT TGAGTTTGAC CTTTCCACGA
    CTTCTTGAGG CAGTTGAGAT GGAAGATGAT GACTTTACGG CCTCTCTGTC AAAGCAGAGT
    TGCATTACTG AACAAACTCA GTATTTTTTC GATAACGACA GTAAATCATT TAGTGGTGTA
    TTAGATTGTG GAAACTGTTC CAGAATCTTC CATGTAGAAA AACTTATGAA CACCAACTTA
    ATATTCATAA TGGTTGAGAG CAAAGGAACT TGTCCCTGTG ACACACGGTT GCTCATACAA
    GCAGAGCAGA CTTCTGATGG TCCAGATCCT TGTGATATGG TGAAGCAACC CAGATACCGA
    AAAGGGCCTG ATGTCTGCTT TGATAACAAT GCTTTGGATC AACTTGTATG CACAACGAAT
    CGTGCCTGTA CCATGATGTC AGCTCCTATC TTCATTTTTC TGCAAGTTTT CATGTGCTGT
    GGATGGTCAG TCACTTTCTC GAATTTTTCT TTGTTTTTGT GTTTGGCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846244.1
    CDS484..3834
    Translation

    Target ORF information:

    RefSeq Version XM_024990476.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGAAC CCCAAATCTC AGGAGCCAGT AACTTTGGAT
    TTCCTTGATG CAGAATTAGA GAACGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAT
    GGAGAAAGCG GAGAAAAAAC ATTCAGGACT CTGGTTAAAT CTCAAGATGA GAGATATATT
    GACAAAGGAA ACAGGACATA TACATGGACT CCTGTCAATG GCACAGATTA CAGTTTGGCC
    TTGGTATTAC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT
    CAGGCCAGAT CCAAAAAGGG CAGAATGAAG GATTCAGAAA CTCTGAAACC GGATAATTTT
    GAAGAGTCTG GCTATACGTT CATAGCACCA AGAGATTACT GCAATGACCT TAAAATATCA
    GATAATAACA CCGAATTTCT TTTAAATTTT AATGAGTTTA TTGATAGAAA AACTCCAAAC
    AACCCATCAT GCAACACAGA CTTGATTAAT AGAGTCTTGC TGGATGCAGG CTTTACAAAT
    GAACTTGTCC AAAATTACTG GAGTAAGCAG AAAAACATCA AGGGAGTGAA AGCACGATTT
    GTTGTGACTG ATGGTGGGAT TACCAGAGTT TATCCGAAAG AGGCTGGAGA AAATTGGCAA
    GAAAACCCAG AAACATATGA GGACAGCTTC TATAAAAGAA GTCTAGATAA CGATAACTAT
    GTTTTCACTG CTCCCTACTT TAACAAAAGT GGACCTGGTG CTTATGAATC AGGCATTATG
    GTAAGCAAAG CTGTAGAAAT TTACATCCAA GGAAAACTTC TTAAACCTGC AGTTGTTGGA
    ATTAAAATTG ATCTAAATTC CTGGATAGAG AATTTCACCA AAACATCAAT CAGGGATCCG
    TGTGCGGGTC CAGTTTGTGA CTGTAAAAGA AACAGTGATG TAATGGACTG TGTTATTCTA
    GATGATGGTG GGTTCCTTTT GATGGCAAAT CATGATGATT ATACTAACCA GATTGGAAGA
    TTTTTTGGAG AGATTGATCC AAGTTTGATG AGACACCTGG TTAATATATC AGTTTATGCT
    TATAACAAGT CTTATGATTA TCAGTCGGTG TGTGAGCCAG GAGCTGCACC AAAACAAGGA
    GCAGGGCATC GCTCAGCATA CGTGCCATCA ATAGCCGACG TATTACACAT TGGCTGGTGG
    GCCACTGCTG CTGCCTGGTC TATTCTACAG CAGTTTCTCT TGAGTTTGAC CTTTCCACGA
    CTTCTTGAGG CAGTTGAGAT GGAAGATGAT GACTTTACGG CCTCTCTGTC AAAGCAGAGT
    TGCATTACTG AACAAACTCA GTATTTTTTC GATAACGACA GTAAATCATT TAGTGGTGTA
    TTAGATTGTG GAAACTGTTC CAGAATCTTC CATGTAGAAA AACTTATGAA CACCAACTTA
    ATATTCATAA TGGTTGAGAG CAAAGGAACT TGTCCCTGTG ACACACGGTT GCTCATACAA
    GCAGAGCAGA CTTCTGATGG TCCAGATCCT TGTGATATGG TGAAGCAACC CAGATACCGA
    AAAGGGCCTG ATGTCTGCTT TGATAACAAT GCTTTGGATC AACTTGTATG CACAACGAAT
    CGTGCCTGTA CCATGATGTC AGCTCCTATC TTCATTTTTC TGCAAGTTTT CATGTGCTGT
    GGATGGTCAG TCACTTTCTC GAATTTTTCT TTGTTTTTGT GTTTGGCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233540
    Clone ID Related Accession (Same CDS sequence) XM_024990477.1
    Accession Version XM_024990477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGGAG CCAGTAACTT TGGATTTCCT TGATGCAGAA
    TTAGAGAACG ATATTAAAGT GGAGATTCGA AATAAAATGA TAGATGGAGA AAGCGGAGAA
    AAAACATTCA GGACTCTGGT TAAATCTCAA GATGAGAGAT ATATTGACAA AGGAAACAGG
    ACATATACAT GGACTCCTGT CAATGGCACA GATTACAGTT TGGCCTTGGT ATTACCAACC
    TACAGTTTTT ACTATATAAA AGCCAAAATA GAAGAGACAA TAACTCAGGC CAGATCCAAA
    AAGGGCAGAA TGAAGGATTC AGAAACTCTG AAACCGGATA ATTTTGAAGA GTCTGGCTAT
    ACGTTCATAG CACCAAGAGA TTACTGCAAT GACCTTAAAA TATCAGATAA TAACACCGAA
    TTTCTTTTAA ATTTTAATGA GTTTATTGAT AGAAAAACTC CAAACAACCC ATCATGCAAC
    ACAGACTTGA TTAATAGAGT CTTGCTGGAT GCAGGCTTTA CAAATGAACT TGTCCAAAAT
    TACTGGAGTA AGCAGAAAAA CATCAAGGGA GTGAAAGCAC GATTTGTTGT GACTGATGGT
    GGGATTACCA GAGTTTATCC GAAAGAGGCT GGAGAAAATT GGCAAGAAAA CCCAGAAACA
    TATGAGGACA GCTTCTATAA AAGAAGTCTA GATAACGATA ACTATGTTTT CACTGCTCCC
    TACTTTAACA AAAGTGGACC TGGTGCTTAT GAATCAGGCA TTATGGTAAG CAAAGCTGTA
    GAAATTTACA TCCAAGGAAA ACTTCTTAAA CCTGCAGTTG TTGGAATTAA AATTGATCTA
    AATTCCTGGA TAGAGAATTT CACCAAAACA TCAATCAGGG ATCCGTGTGC GGGTCCAGTT
    TGTGACTGTA AAAGAAACAG TGATGTAATG GACTGTGTTA TTCTAGATGA TGGTGGGTTC
    CTTTTGATGG CAAATCATGA TGATTATACT AACCAGATTG GAAGATTTTT TGGAGAGATT
    GATCCAAGTT TGATGAGACA CCTGGTTAAT ATATCAGTTT ATGCTTATAA CAAGTCTTAT
    GATTATCAGT CGGTGTGTGA GCCAGGAGCT GCACCAAAAC AAGGAGCAGG GCATCGCTCA
    GCATACGTGC CATCAATAGC CGACGTATTA CACATTGGCT GGTGGGCCAC TGCTGCTGCC
    TGGTCTATTC TACAGCAGTT TCTCTTGAGT TTGACCTTTC CACGACTTCT TGAGGCAGTT
    GAGATGGAAG ATGATGACTT TACGGCCTCT CTGTCAAAGC AGAGTTGCAT TACTGAACAA
    ACTCAGTATT TTTTCGATAA CGACAGTAAA TCATTTAGTG GTGTATTAGA TTGTGGAAAC
    TGTTCCAGAA TCTTCCATGT AGAAAAACTT ATGAACACCA ACTTAATATT CATAATGGTT
    GAGAGCAAAG GAACTTGTCC CTGTGACACA CGGTTGCTCA TACAAGCAGA GCAGACTTCT
    GATGGTCCAG ATCCTTGTGA TATGGTGAAG CAACCCAGAT ACCGAAAAGG GCCTGATGTC
    TGCTTTGATA ACAATGCTTT GGATCAACTT GTATGCACAA CGAATCGTGC CTGTACCATG
    ATGTCAGCTC CTATCTTCAT TTTTCTGCAA GTTTTCATGT GCTGTGGATG GTCAGTCACT
    TTCTCGAATT TTTCTTTGTT TTTGTGTTTG GCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846245.1
    CDS484..3819
    Translation

    Target ORF information:

    RefSeq Version XM_024990477.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGGAG CCAGTAACTT TGGATTTCCT TGATGCAGAA
    TTAGAGAACG ATATTAAAGT GGAGATTCGA AATAAAATGA TAGATGGAGA AAGCGGAGAA
    AAAACATTCA GGACTCTGGT TAAATCTCAA GATGAGAGAT ATATTGACAA AGGAAACAGG
    ACATATACAT GGACTCCTGT CAATGGCACA GATTACAGTT TGGCCTTGGT ATTACCAACC
    TACAGTTTTT ACTATATAAA AGCCAAAATA GAAGAGACAA TAACTCAGGC CAGATCCAAA
    AAGGGCAGAA TGAAGGATTC AGAAACTCTG AAACCGGATA ATTTTGAAGA GTCTGGCTAT
    ACGTTCATAG CACCAAGAGA TTACTGCAAT GACCTTAAAA TATCAGATAA TAACACCGAA
    TTTCTTTTAA ATTTTAATGA GTTTATTGAT AGAAAAACTC CAAACAACCC ATCATGCAAC
    ACAGACTTGA TTAATAGAGT CTTGCTGGAT GCAGGCTTTA CAAATGAACT TGTCCAAAAT
    TACTGGAGTA AGCAGAAAAA CATCAAGGGA GTGAAAGCAC GATTTGTTGT GACTGATGGT
    GGGATTACCA GAGTTTATCC GAAAGAGGCT GGAGAAAATT GGCAAGAAAA CCCAGAAACA
    TATGAGGACA GCTTCTATAA AAGAAGTCTA GATAACGATA ACTATGTTTT CACTGCTCCC
    TACTTTAACA AAAGTGGACC TGGTGCTTAT GAATCAGGCA TTATGGTAAG CAAAGCTGTA
    GAAATTTACA TCCAAGGAAA ACTTCTTAAA CCTGCAGTTG TTGGAATTAA AATTGATCTA
    AATTCCTGGA TAGAGAATTT CACCAAAACA TCAATCAGGG ATCCGTGTGC GGGTCCAGTT
    TGTGACTGTA AAAGAAACAG TGATGTAATG GACTGTGTTA TTCTAGATGA TGGTGGGTTC
    CTTTTGATGG CAAATCATGA TGATTATACT AACCAGATTG GAAGATTTTT TGGAGAGATT
    GATCCAAGTT TGATGAGACA CCTGGTTAAT ATATCAGTTT ATGCTTATAA CAAGTCTTAT
    GATTATCAGT CGGTGTGTGA GCCAGGAGCT GCACCAAAAC AAGGAGCAGG GCATCGCTCA
    GCATACGTGC CATCAATAGC CGACGTATTA CACATTGGCT GGTGGGCCAC TGCTGCTGCC
    TGGTCTATTC TACAGCAGTT TCTCTTGAGT TTGACCTTTC CACGACTTCT TGAGGCAGTT
    GAGATGGAAG ATGATGACTT TACGGCCTCT CTGTCAAAGC AGAGTTGCAT TACTGAACAA
    ACTCAGTATT TTTTCGATAA CGACAGTAAA TCATTTAGTG GTGTATTAGA TTGTGGAAAC
    TGTTCCAGAA TCTTCCATGT AGAAAAACTT ATGAACACCA ACTTAATATT CATAATGGTT
    GAGAGCAAAG GAACTTGTCC CTGTGACACA CGGTTGCTCA TACAAGCAGA GCAGACTTCT
    GATGGTCCAG ATCCTTGTGA TATGGTGAAG CAACCCAGAT ACCGAAAAGG GCCTGATGTC
    TGCTTTGATA ACAATGCTTT GGATCAACTT GTATGCACAA CGAATCGTGC CTGTACCATG
    ATGTCAGCTC CTATCTTCAT TTTTCTGCAA GTTTTCATGT GCTGTGGATG GTCAGTCACT
    TTCTCGAATT TTTCTTTGTT TTTGTGTTTG GCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233541
    Clone ID Related Accession (Same CDS sequence) XM_024990478.1
    Accession Version XM_024990478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGAAC CCCAAATCTC AGGAGCCAGT AACTTTGGAT
    TTCCTTGATG CAGAATTAGA GAACGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAT
    GGAGAAAGCG GAGAAAAAAC ATTCAGGACT CTGGTTAAAT CTCAAGATGA GAGATATATT
    GACAAAGGAA ACAGGACATA TACATGGACT CCTGTCAATG GCACAGATTA CAGTTTGGCC
    TTGGTATTAC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT
    CAGGCCAGAT CCAAAAAGGG CAGAATGAAG GATTCAGAAA CTCTGAAACC GGATAATTTT
    GAAGAGTCTG GCTATACGTT CATAGCACCA AGAGATTACT GCAATGACCT TAAAATATCA
    GATAATAACA CCGAATTTCT TTTAAATTTT AATGAGTTTA TTGATAGAAA AACTCCAAAC
    AACCCATCAT GCAACACAGA CTTGATTAAT AGAGTCTTGC TGGATGCAGG CTTTACAAAT
    GAACTTGTCC AAAATTACTG GAGTAAGCAG AAAAACATCA AGGGAGTGAA AGCACGATTT
    GTTGTGACTG ATGGTGGGAT TACCAGAGTT TATCCGAAAG AGGCTGGAGA AAATTGGCAA
    GAAAACCCAG AAACATATGA GGACAGCTTC TATAAAAGAA GTCTAGATAA CGATAACTAT
    GTTTTCACTG CTCCCTACTT TAACAAAAGT GGACCTGGTG CTTATGAATC AGGCATTATG
    GTAAGCAAAG CTGTAGAAAT TTACATCCAA GGAAAACTTC TTAAACCTGC AGTTGTTGGA
    ATTAAAATTG ATCTAAATTC CTGGATAGAG AATTTCACCA AAACATCAAT CAGGGATCCG
    TGTGCGGGTC CAGTTTGTGA CTGTAAAAGA AACAGTGATG TAATGGACTG TGTTATTCTA
    GATGATGGTG GGTTCCTTTT GATGGCAAAT CATGATGATT ATACTAACCA GATTGGAAGA
    TTTTTTGGAG AGATTGATCC AAGTTTGATG AGACACCTGG TTAATATATC AGTTTATGCT
    TATAACAAGT CTTATGATTA TCAGTCGGTG TGTGAGCCAG GAGCTGCACC AAAACAAGGA
    GCAGGGCATC GCTCAGCATA CGTGCCATCA ATAGCCGACG TATTACACAT TGGCTGGTGG
    GCCACTGCTG CTGCCTGGTC TATTCTACAG CAGTTTCTCT TGAGTTTGAC CTTTCCACGA
    CTTCTTGAGG CAGTTGAGAT GGAAGATGAT GACTTTACGG CCTCTCTGTC AAAGCAGAGT
    TGCATTACTG AACAAACTCA GTATTTTTTC GATAACGACA GTAAATCATT TAGTGGTGTA
    TTAGATTGTG GAAACTGTTC CAGAATCTTC CATGTAGAAA AACTTATGAA CACCAACTTA
    ATATTCATAA TGGTTGAGAG CAAAGGAACT TGTCCCTGTG ACACACGGTT GCTCATACAA
    GCAGAGCAGA CTTCTGATGG TCCAGATCCT TGTGATATGG TGAAGCAACC CAGATACCGA
    AAAGGGCCTG ATGTCTGCTT TGATAACAAT GCTTTGGAGG ACTATACCGA CTGTGGTGGT
    GTCTCTGGAT TAAACCCCTC CCTGTGGTCC ATCATCGGCA TCCAGTGTGT GCTACTTTGG
    CTCTTATCTG GCAGCAGACA CCACCAGTTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846246.1
    CDS484..3816
    Translation

    Target ORF information:

    RefSeq Version XM_024990478.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGAAC CCCAAATCTC AGGAGCCAGT AACTTTGGAT
    TTCCTTGATG CAGAATTAGA GAACGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAT
    GGAGAAAGCG GAGAAAAAAC ATTCAGGACT CTGGTTAAAT CTCAAGATGA GAGATATATT
    GACAAAGGAA ACAGGACATA TACATGGACT CCTGTCAATG GCACAGATTA CAGTTTGGCC
    TTGGTATTAC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT
    CAGGCCAGAT CCAAAAAGGG CAGAATGAAG GATTCAGAAA CTCTGAAACC GGATAATTTT
    GAAGAGTCTG GCTATACGTT CATAGCACCA AGAGATTACT GCAATGACCT TAAAATATCA
    GATAATAACA CCGAATTTCT TTTAAATTTT AATGAGTTTA TTGATAGAAA AACTCCAAAC
    AACCCATCAT GCAACACAGA CTTGATTAAT AGAGTCTTGC TGGATGCAGG CTTTACAAAT
    GAACTTGTCC AAAATTACTG GAGTAAGCAG AAAAACATCA AGGGAGTGAA AGCACGATTT
    GTTGTGACTG ATGGTGGGAT TACCAGAGTT TATCCGAAAG AGGCTGGAGA AAATTGGCAA
    GAAAACCCAG AAACATATGA GGACAGCTTC TATAAAAGAA GTCTAGATAA CGATAACTAT
    GTTTTCACTG CTCCCTACTT TAACAAAAGT GGACCTGGTG CTTATGAATC AGGCATTATG
    GTAAGCAAAG CTGTAGAAAT TTACATCCAA GGAAAACTTC TTAAACCTGC AGTTGTTGGA
    ATTAAAATTG ATCTAAATTC CTGGATAGAG AATTTCACCA AAACATCAAT CAGGGATCCG
    TGTGCGGGTC CAGTTTGTGA CTGTAAAAGA AACAGTGATG TAATGGACTG TGTTATTCTA
    GATGATGGTG GGTTCCTTTT GATGGCAAAT CATGATGATT ATACTAACCA GATTGGAAGA
    TTTTTTGGAG AGATTGATCC AAGTTTGATG AGACACCTGG TTAATATATC AGTTTATGCT
    TATAACAAGT CTTATGATTA TCAGTCGGTG TGTGAGCCAG GAGCTGCACC AAAACAAGGA
    GCAGGGCATC GCTCAGCATA CGTGCCATCA ATAGCCGACG TATTACACAT TGGCTGGTGG
    GCCACTGCTG CTGCCTGGTC TATTCTACAG CAGTTTCTCT TGAGTTTGAC CTTTCCACGA
    CTTCTTGAGG CAGTTGAGAT GGAAGATGAT GACTTTACGG CCTCTCTGTC AAAGCAGAGT
    TGCATTACTG AACAAACTCA GTATTTTTTC GATAACGACA GTAAATCATT TAGTGGTGTA
    TTAGATTGTG GAAACTGTTC CAGAATCTTC CATGTAGAAA AACTTATGAA CACCAACTTA
    ATATTCATAA TGGTTGAGAG CAAAGGAACT TGTCCCTGTG ACACACGGTT GCTCATACAA
    GCAGAGCAGA CTTCTGATGG TCCAGATCCT TGTGATATGG TGAAGCAACC CAGATACCGA
    AAAGGGCCTG ATGTCTGCTT TGATAACAAT GCTTTGGAGG ACTATACCGA CTGTGGTGGT
    GTCTCTGGAT TAAACCCCTC CCTGTGGTCC ATCATCGGCA TCCAGTGTGT GCTACTTTGG
    CTCTTATCTG GCAGCAGACA CCACCAGTTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233542
    Clone ID Related Accession (Same CDS sequence) XM_024990479.1
    Accession Version XM_024990479.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGAAC CCCAAATCTC AGGAGCCAGT AACTTTGGAT
    TTCCTTGATG CAGAATTAGA GAACGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAT
    GGAGAAAGCG GAGAAAAAAC ATTCAGGACT CTGGTTAAAT CTCAAGATGA GAGATATATT
    GACAAAGGAA ACAGGACATA TACATGGACT CCTGTCAATG GCACAGATTA CAGTTTGGCC
    TTGGTATTAC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT
    CAGGCCAGAT ATTCAGAAAC TCTGAAACCG GATAATTTTG AAGAGTCTGG CTATACGTTC
    ATAGCACCAA GAGATTACTG CAATGACCTT AAAATATCAG ATAATAACAC CGAATTTCTT
    TTAAATTTTA ATGAGTTTAT TGATAGAAAA ACTCCAAACA ACCCATCATG CAACACAGAC
    TTGATTAATA GAGTCTTGCT GGATGCAGGC TTTACAAATG AACTTGTCCA AAATTACTGG
    AGTAAGCAGA AAAACATCAA GGGAGTGAAA GCACGATTTG TTGTGACTGA TGGTGGGATT
    ACCAGAGTTT ATCCGAAAGA GGCTGGAGAA AATTGGCAAG AAAACCCAGA AACATATGAG
    GACAGCTTCT ATAAAAGAAG TCTAGATAAC GATAACTATG TTTTCACTGC TCCCTACTTT
    AACAAAAGTG GACCTGGTGC TTATGAATCA GGCATTATGG TAAGCAAAGC TGTAGAAATT
    TACATCCAAG GAAAACTTCT TAAACCTGCA GTTGTTGGAA TTAAAATTGA TCTAAATTCC
    TGGATAGAGA ATTTCACCAA AACATCAATC AGGGATCCGT GTGCGGGTCC AGTTTGTGAC
    TGTAAAAGAA ACAGTGATGT AATGGACTGT GTTATTCTAG ATGATGGTGG GTTCCTTTTG
    ATGGCAAATC ATGATGATTA TACTAACCAG ATTGGAAGAT TTTTTGGAGA GATTGATCCA
    AGTTTGATGA GACACCTGGT TAATATATCA GTTTATGCTT ATAACAAGTC TTATGATTAT
    CAGTCGGTGT GTGAGCCAGG AGCTGCACCA AAACAAGGAG CAGGGCATCG CTCAGCATAC
    GTGCCATCAA TAGCCGACGT ATTACACATT GGCTGGTGGG CCACTGCTGC TGCCTGGTCT
    ATTCTACAGC AGTTTCTCTT GAGTTTGACC TTTCCACGAC TTCTTGAGGC AGTTGAGATG
    GAAGATGATG ACTTTACGGC CTCTCTGTCA AAGCAGAGTT GCATTACTGA ACAAACTCAG
    TATTTTTTCG ATAACGACAG TAAATCATTT AGTGGTGTAT TAGATTGTGG AAACTGTTCC
    AGAATCTTCC ATGTAGAAAA ACTTATGAAC ACCAACTTAA TATTCATAAT GGTTGAGAGC
    AAAGGAACTT GTCCCTGTGA CACACGGTTG CTCATACAAG CAGAGCAGAC TTCTGATGGT
    CCAGATCCTT GTGATATGGT GAAGCAACCC AGATACCGAA AAGGGCCTGA TGTCTGCTTT
    GATAACAATG CTTTGGATCA ACTTGTATGC ACAACGAATC GTGCCTGTAC CATGATGTCA
    GCTCCTATCT TCATTTTTCT GCAAGTTTTC ATGTGCTGTG GATGGTCAGT CACTTTCTCG
    AATTTTTCTT TGTTTTTGTG TTTGGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846247.1
    CDS484..3813
    Translation

    Target ORF information:

    RefSeq Version XM_024990479.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990479.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGAAC CCCAAATCTC AGGAGCCAGT AACTTTGGAT
    TTCCTTGATG CAGAATTAGA GAACGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAT
    GGAGAAAGCG GAGAAAAAAC ATTCAGGACT CTGGTTAAAT CTCAAGATGA GAGATATATT
    GACAAAGGAA ACAGGACATA TACATGGACT CCTGTCAATG GCACAGATTA CAGTTTGGCC
    TTGGTATTAC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT
    CAGGCCAGAT ATTCAGAAAC TCTGAAACCG GATAATTTTG AAGAGTCTGG CTATACGTTC
    ATAGCACCAA GAGATTACTG CAATGACCTT AAAATATCAG ATAATAACAC CGAATTTCTT
    TTAAATTTTA ATGAGTTTAT TGATAGAAAA ACTCCAAACA ACCCATCATG CAACACAGAC
    TTGATTAATA GAGTCTTGCT GGATGCAGGC TTTACAAATG AACTTGTCCA AAATTACTGG
    AGTAAGCAGA AAAACATCAA GGGAGTGAAA GCACGATTTG TTGTGACTGA TGGTGGGATT
    ACCAGAGTTT ATCCGAAAGA GGCTGGAGAA AATTGGCAAG AAAACCCAGA AACATATGAG
    GACAGCTTCT ATAAAAGAAG TCTAGATAAC GATAACTATG TTTTCACTGC TCCCTACTTT
    AACAAAAGTG GACCTGGTGC TTATGAATCA GGCATTATGG TAAGCAAAGC TGTAGAAATT
    TACATCCAAG GAAAACTTCT TAAACCTGCA GTTGTTGGAA TTAAAATTGA TCTAAATTCC
    TGGATAGAGA ATTTCACCAA AACATCAATC AGGGATCCGT GTGCGGGTCC AGTTTGTGAC
    TGTAAAAGAA ACAGTGATGT AATGGACTGT GTTATTCTAG ATGATGGTGG GTTCCTTTTG
    ATGGCAAATC ATGATGATTA TACTAACCAG ATTGGAAGAT TTTTTGGAGA GATTGATCCA
    AGTTTGATGA GACACCTGGT TAATATATCA GTTTATGCTT ATAACAAGTC TTATGATTAT
    CAGTCGGTGT GTGAGCCAGG AGCTGCACCA AAACAAGGAG CAGGGCATCG CTCAGCATAC
    GTGCCATCAA TAGCCGACGT ATTACACATT GGCTGGTGGG CCACTGCTGC TGCCTGGTCT
    ATTCTACAGC AGTTTCTCTT GAGTTTGACC TTTCCACGAC TTCTTGAGGC AGTTGAGATG
    GAAGATGATG ACTTTACGGC CTCTCTGTCA AAGCAGAGTT GCATTACTGA ACAAACTCAG
    TATTTTTTCG ATAACGACAG TAAATCATTT AGTGGTGTAT TAGATTGTGG AAACTGTTCC
    AGAATCTTCC ATGTAGAAAA ACTTATGAAC ACCAACTTAA TATTCATAAT GGTTGAGAGC
    AAAGGAACTT GTCCCTGTGA CACACGGTTG CTCATACAAG CAGAGCAGAC TTCTGATGGT
    CCAGATCCTT GTGATATGGT GAAGCAACCC AGATACCGAA AAGGGCCTGA TGTCTGCTTT
    GATAACAATG CTTTGGATCA ACTTGTATGC ACAACGAATC GTGCCTGTAC CATGATGTCA
    GCTCCTATCT TCATTTTTCT GCAAGTTTTC ATGTGCTGTG GATGGTCAGT CACTTTCTCG
    AATTTTTCTT TGTTTTTGTG TTTGGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233543
    Clone ID Related Accession (Same CDS sequence) XM_024990480.1
    Accession Version XM_024990480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGAAC CCCAAATCTC AGGAGCCAGT AACTTTGGAT
    TTCCTTGATG CAGAATTAGA GAACGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAT
    GGAGAAAGCG GAGAAAAAAC ATTCAGGACT CTGGTTAAAT CTCAAGATGA GAGATATATT
    GACAAAGGAA ACAGGACATA TACATGGACT CCTGTCAATG GCACAGATTA CAGTTTGGCC
    TTGGTATTAC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT
    CAGGCCAGAT ATTCAGAAAC TCTGAAACCG GATAATTTTG AAGAGTCTGG CTATACGTTC
    ATAGCACCAA GAGATTACTG CAATGACCTT AAAATATCAG ATAATAACAC CGAATTTCTT
    TTAAATTTTA ATGAGTTTAT TGATAGAAAA ACTCCAAACA ACCCATCATG CAACACAGAC
    TTGATTAATA GAGTCTTGCT GGATGCAGGC TTTACAAATG AACTTGTCCA AAATTACTGG
    AGTAAGCAGA AAAACATCAA GGGAGTGAAA GCACGATTTG TTGTGACTGA TGGTGGGATT
    ACCAGAGTTT ATCCGAAAGA GGCTGGAGAA AATTGGCAAG AAAACCCAGA AACATATGAG
    GACAGCTTCT ATAAAAGAAG TCTAGATAAC GATAACTATG TTTTCACTGC TCCCTACTTT
    AACAAAAGTG GACCTGGTGC TTATGAATCA GGCATTATGG TAAGCAAAGC TGTAGAAATT
    TACATCCAAG GAAAACTTCT TAAACCTGCA GTTGTTGGAA TTAAAATTGA TCTAAATTCC
    TGGATAGAGA ATTTCACCAA AACATCAATC AGGGATCCGT GTGCGGGTCC AGTTTGTGAC
    TGTAAAAGAA ACAGTGATGT AATGGACTGT GTTATTCTAG ATGATGGTGG GTTCCTTTTG
    ATGGCAAATC ATGATGATTA TACTAACCAG ATTGGAAGAT TTTTTGGAGA GATTGATCCA
    AGTTTGATGA GACACCTGGT TAATATATCA GTTTATGCTT ATAACAAGTC TTATGATTAT
    CAGTCGGTGT GTGAGCCAGG AGCTGCACCA AAACAAGGAG CAGGGCATCG CTCAGCATAC
    GTGCCATCAA TAGCCGACGT ATTACACATT GGCTGGTGGG CCACTGCTGC TGCCTGGTCT
    ATTCTACAGC AGTTTCTCTT GAGTTTGACC TTTCCACGAC TTCTTGAGGC AGTTGAGATG
    GAAGATGATG ACTTTACGGC CTCTCTGTCA AAGCAGAGTT GCATTACTGA ACAAACTCAG
    TATTTTTTCG ATAACGACAG TAAATCATTT AGTGGTGTAT TAGATTGTGG AAACTGTTCC
    AGAATCTTCC ATGTAGAAAA ACTTATGAAC ACCAACTTAA TATTCATAAT GGTTGAGAGC
    AAAGGAACTT GTCCCTGTGA CACACGGTTG CTCATACAAG CAGAGCAGAC TTCTGATGGT
    CCAGATCCTT GTGATATGGT GAAGCAACCC AGATACCGAA AAGGGCCTGA TGTCTGCTTT
    GATAACAATG CTTTGGAGGA CTATACCGAC TGTGGTGGTG TCTCTGGATT AAACCCCTCC
    CTGTGGTCCA TCATCGGCAT CCAGTGTGTG CTACTTTGGC TCTTATCTGG CAGCAGACAC
    CACCAGTTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846248.1
    CDS484..3795
    Translation

    Target ORF information:

    RefSeq Version XM_024990480.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG CCTATTGGCT TAGGTATACC AACAATTAAT
    TTAAGAAAAA GGAGACCCAA TGTTCAGAAC CCCAAATCTC AGGAGCCAGT AACTTTGGAT
    TTCCTTGATG CAGAATTAGA GAACGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAT
    GGAGAAAGCG GAGAAAAAAC ATTCAGGACT CTGGTTAAAT CTCAAGATGA GAGATATATT
    GACAAAGGAA ACAGGACATA TACATGGACT CCTGTCAATG GCACAGATTA CAGTTTGGCC
    TTGGTATTAC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT
    CAGGCCAGAT ATTCAGAAAC TCTGAAACCG GATAATTTTG AAGAGTCTGG CTATACGTTC
    ATAGCACCAA GAGATTACTG CAATGACCTT AAAATATCAG ATAATAACAC CGAATTTCTT
    TTAAATTTTA ATGAGTTTAT TGATAGAAAA ACTCCAAACA ACCCATCATG CAACACAGAC
    TTGATTAATA GAGTCTTGCT GGATGCAGGC TTTACAAATG AACTTGTCCA AAATTACTGG
    AGTAAGCAGA AAAACATCAA GGGAGTGAAA GCACGATTTG TTGTGACTGA TGGTGGGATT
    ACCAGAGTTT ATCCGAAAGA GGCTGGAGAA AATTGGCAAG AAAACCCAGA AACATATGAG
    GACAGCTTCT ATAAAAGAAG TCTAGATAAC GATAACTATG TTTTCACTGC TCCCTACTTT
    AACAAAAGTG GACCTGGTGC TTATGAATCA GGCATTATGG TAAGCAAAGC TGTAGAAATT
    TACATCCAAG GAAAACTTCT TAAACCTGCA GTTGTTGGAA TTAAAATTGA TCTAAATTCC
    TGGATAGAGA ATTTCACCAA AACATCAATC AGGGATCCGT GTGCGGGTCC AGTTTGTGAC
    TGTAAAAGAA ACAGTGATGT AATGGACTGT GTTATTCTAG ATGATGGTGG GTTCCTTTTG
    ATGGCAAATC ATGATGATTA TACTAACCAG ATTGGAAGAT TTTTTGGAGA GATTGATCCA
    AGTTTGATGA GACACCTGGT TAATATATCA GTTTATGCTT ATAACAAGTC TTATGATTAT
    CAGTCGGTGT GTGAGCCAGG AGCTGCACCA AAACAAGGAG CAGGGCATCG CTCAGCATAC
    GTGCCATCAA TAGCCGACGT ATTACACATT GGCTGGTGGG CCACTGCTGC TGCCTGGTCT
    ATTCTACAGC AGTTTCTCTT GAGTTTGACC TTTCCACGAC TTCTTGAGGC AGTTGAGATG
    GAAGATGATG ACTTTACGGC CTCTCTGTCA AAGCAGAGTT GCATTACTGA ACAAACTCAG
    TATTTTTTCG ATAACGACAG TAAATCATTT AGTGGTGTAT TAGATTGTGG AAACTGTTCC
    AGAATCTTCC ATGTAGAAAA ACTTATGAAC ACCAACTTAA TATTCATAAT GGTTGAGAGC
    AAAGGAACTT GTCCCTGTGA CACACGGTTG CTCATACAAG CAGAGCAGAC TTCTGATGGT
    CCAGATCCTT GTGATATGGT GAAGCAACCC AGATACCGAA AAGGGCCTGA TGTCTGCTTT
    GATAACAATG CTTTGGAGGA CTATACCGAC TGTGGTGGTG TCTCTGGATT AAACCCCTCC
    CTGTGGTCCA TCATCGGCAT CCAGTGTGTG CTACTTTGGC TCTTATCTGG CAGCAGACAC
    CACCAGTTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233544
    Clone ID Related Accession (Same CDS sequence) XM_024990481.1
    Accession Version XM_024990481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAACG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG ATCAACTTGT ATGCACAACG
    AATCGTGCCT GTACCATGAT GTCAGCTCCT ATCTTCATTT TTCTGCAAGT TTTCATGTGC
    TGTGGATGGT CAGTCACTTT CTCGAATTTT TCTTTGTTTT TGTGTTTGGC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846249.1
    CDS484..3777
    Translation

    Target ORF information:

    RefSeq Version XM_024990481.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAACG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG ATCAACTTGT ATGCACAACG
    AATCGTGCCT GTACCATGAT GTCAGCTCCT ATCTTCATTT TTCTGCAAGT TTTCATGTGC
    TGTGGATGGT CAGTCACTTT CTCGAATTTT TCTTTGTTTT TGTGTTTGGC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233545
    Clone ID Related Accession (Same CDS sequence) XM_024990482.1
    Accession Version XM_024990482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG GAGCCAGTAA CTTTGGATTT CCTTGATGCA
    GAATTAGAGA ACGATATTAA AGTGGAGATT CGAAATAAAA TGATAGATGG AGAAAGCGGA
    GAAAAAACAT TCAGGACTCT GGTTAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAC
    AGGACATATA CATGGACTCC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTACCA
    ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATCC
    AAAAAGGGCA GAATGAAGGA TTCAGAAACT CTGAAACCGG ATAATTTTGA AGAGTCTGGC
    TATACGTTCA TAGCACCAAG AGATTACTGC AATGACCTTA AAATATCAGA TAATAACACC
    GAATTTCTTT TAAATTTTAA TGAGTTTATT GATAGAAAAA CTCCAAACAA CCCATCATGC
    AACACAGACT TGATTAATAG AGTCTTGCTG GATGCAGGCT TTACAAATGA ACTTGTCCAA
    AATTACTGGA GTAAGCAGAA AAACATCAAG GGAGTGAAAG CACGATTTGT TGTGACTGAT
    GGTGGGATTA CCAGAGTTTA TCCGAAAGAG GCTGGAGAAA ATTGGCAAGA AAACCCAGAA
    ACATATGAGG ACAGCTTCTA TAAAAGAAGT CTAGATAACG ATAACTATGT TTTCACTGCT
    CCCTACTTTA ACAAAAGTGG ACCTGGTGCT TATGAATCAG GCATTATGGT AAGCAAAGCT
    GTAGAAATTT ACATCCAAGG AAAACTTCTT AAACCTGCAG TTGTTGGAAT TAAAATTGAT
    CTAAATTCCT GGATAGAGAA TTTCACCAAA ACATCAATCA GGGATCCGTG TGCGGGTCCA
    GTTTGTGACT GTAAAAGAAA CAGTGATGTA ATGGACTGTG TTATTCTAGA TGATGGTGGG
    TTCCTTTTGA TGGCAAATCA TGATGATTAT ACTAACCAGA TTGGAAGATT TTTTGGAGAG
    ATTGATCCAA GTTTGATGAG ACACCTGGTT AATATATCAG TTTATGCTTA TAACAAGTCT
    TATGATTATC AGTCGGTGTG TGAGCCAGGA GCTGCACCAA AACAAGGAGC AGGGCATCGC
    TCAGCATACG TGCCATCAAT AGCCGACGTA TTACACATTG GCTGGTGGGC CACTGCTGCT
    GCCTGGTCTA TTCTACAGCA GTTTCTCTTG AGTTTGACCT TTCCACGACT TCTTGAGGCA
    GTTGAGATGG AAGATGATGA CTTTACGGCC TCTCTGTCAA AGCAGAGTTG CATTACTGAA
    CAAACTCAGT ATTTTTTCGA TAACGACAGT AAATCATTTA GTGGTGTATT AGATTGTGGA
    AACTGTTCCA GAATCTTCCA TGTAGAAAAA CTTATGAACA CCAACTTAAT ATTCATAATG
    GTTGAGAGCA AAGGAACTTG TCCCTGTGAC ACACGGTTGC TCATACAAGC AGAGCAGACT
    TCTGATGGTC CAGATCCTTG TGATATGGTG AAGCAACCCA GATACCGAAA AGGGCCTGAT
    GTCTGCTTTG ATAACAATGC TTTGGATCAA CTTGTATGCA CAACGAATCG TGCCTGTACC
    ATGATGTCAG CTCCTATCTT CATTTTTCTG CAAGTTTTCA TGTGCTGTGG ATGGTCAGTC
    ACTTTCTCGA ATTTTTCTTT GTTTTTGTGT TTGGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846250.1
    CDS484..3762
    Translation

    Target ORF information:

    RefSeq Version XM_024990482.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG GAGCCAGTAA CTTTGGATTT CCTTGATGCA
    GAATTAGAGA ACGATATTAA AGTGGAGATT CGAAATAAAA TGATAGATGG AGAAAGCGGA
    GAAAAAACAT TCAGGACTCT GGTTAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAC
    AGGACATATA CATGGACTCC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTACCA
    ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATCC
    AAAAAGGGCA GAATGAAGGA TTCAGAAACT CTGAAACCGG ATAATTTTGA AGAGTCTGGC
    TATACGTTCA TAGCACCAAG AGATTACTGC AATGACCTTA AAATATCAGA TAATAACACC
    GAATTTCTTT TAAATTTTAA TGAGTTTATT GATAGAAAAA CTCCAAACAA CCCATCATGC
    AACACAGACT TGATTAATAG AGTCTTGCTG GATGCAGGCT TTACAAATGA ACTTGTCCAA
    AATTACTGGA GTAAGCAGAA AAACATCAAG GGAGTGAAAG CACGATTTGT TGTGACTGAT
    GGTGGGATTA CCAGAGTTTA TCCGAAAGAG GCTGGAGAAA ATTGGCAAGA AAACCCAGAA
    ACATATGAGG ACAGCTTCTA TAAAAGAAGT CTAGATAACG ATAACTATGT TTTCACTGCT
    CCCTACTTTA ACAAAAGTGG ACCTGGTGCT TATGAATCAG GCATTATGGT AAGCAAAGCT
    GTAGAAATTT ACATCCAAGG AAAACTTCTT AAACCTGCAG TTGTTGGAAT TAAAATTGAT
    CTAAATTCCT GGATAGAGAA TTTCACCAAA ACATCAATCA GGGATCCGTG TGCGGGTCCA
    GTTTGTGACT GTAAAAGAAA CAGTGATGTA ATGGACTGTG TTATTCTAGA TGATGGTGGG
    TTCCTTTTGA TGGCAAATCA TGATGATTAT ACTAACCAGA TTGGAAGATT TTTTGGAGAG
    ATTGATCCAA GTTTGATGAG ACACCTGGTT AATATATCAG TTTATGCTTA TAACAAGTCT
    TATGATTATC AGTCGGTGTG TGAGCCAGGA GCTGCACCAA AACAAGGAGC AGGGCATCGC
    TCAGCATACG TGCCATCAAT AGCCGACGTA TTACACATTG GCTGGTGGGC CACTGCTGCT
    GCCTGGTCTA TTCTACAGCA GTTTCTCTTG AGTTTGACCT TTCCACGACT TCTTGAGGCA
    GTTGAGATGG AAGATGATGA CTTTACGGCC TCTCTGTCAA AGCAGAGTTG CATTACTGAA
    CAAACTCAGT ATTTTTTCGA TAACGACAGT AAATCATTTA GTGGTGTATT AGATTGTGGA
    AACTGTTCCA GAATCTTCCA TGTAGAAAAA CTTATGAACA CCAACTTAAT ATTCATAATG
    GTTGAGAGCA AAGGAACTTG TCCCTGTGAC ACACGGTTGC TCATACAAGC AGAGCAGACT
    TCTGATGGTC CAGATCCTTG TGATATGGTG AAGCAACCCA GATACCGAAA AGGGCCTGAT
    GTCTGCTTTG ATAACAATGC TTTGGATCAA CTTGTATGCA CAACGAATCG TGCCTGTACC
    ATGATGTCAG CTCCTATCTT CATTTTTCTG CAAGTTTTCA TGTGCTGTGG ATGGTCAGTC
    ACTTTCTCGA ATTTTTCTTT GTTTTTGTGT TTGGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233546
    Clone ID Related Accession (Same CDS sequence) XM_024990483.1
    Accession Version XM_024990483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATATTCAGA AACTCTGAAA CCGGATAATT TTGAAGAGTC TGGCTATACG
    TTCATAGCAC CAAGAGATTA CTGCAATGAC CTTAAAATAT CAGATAATAA CACCGAATTT
    CTTTTAAATT TTAATGAGTT TATTGATAGA AAAACTCCAA ACAACCCATC ATGCAACACA
    GACTTGATTA ATAGAGTCTT GCTGGATGCA GGCTTTACAA ATGAACTTGT CCAAAATTAC
    TGGAGTAAGC AGAAAAACAT CAAGGGAGTG AAAGCACGAT TTGTTGTGAC TGATGGTGGG
    ATTACCAGAG TTTATCCGAA AGAGGCTGGA GAAAATTGGC AAGAAAACCC AGAAACATAT
    GAGGACAGCT TCTATAAAAG AAGTCTAGAT AACGATAACT ATGTTTTCAC TGCTCCCTAC
    TTTAACAAAA GTGGACCTGG TGCTTATGAA TCAGGCATTA TGGTAAGCAA AGCTGTAGAA
    ATTTACATCC AAGGAAAACT TCTTAAACCT GCAGTTGTTG GAATTAAAAT TGATCTAAAT
    TCCTGGATAG AGAATTTCAC CAAAACATCA ATCAGGGATC CGTGTGCGGG TCCAGTTTGT
    GACTGTAAAA GAAACAGTGA TGTAATGGAC TGTGTTATTC TAGATGATGG TGGGTTCCTT
    TTGATGGCAA ATCATGATGA TTATACTAAC CAGATTGGAA GATTTTTTGG AGAGATTGAT
    CCAAGTTTGA TGAGACACCT GGTTAATATA TCAGTTTATG CTTATAACAA GTCTTATGAT
    TATCAGTCGG TGTGTGAGCC AGGAGCTGCA CCAAAACAAG GAGCAGGGCA TCGCTCAGCA
    TACGTGCCAT CAATAGCCGA CGTATTACAC ATTGGCTGGT GGGCCACTGC TGCTGCCTGG
    TCTATTCTAC AGCAGTTTCT CTTGAGTTTG ACCTTTCCAC GACTTCTTGA GGCAGTTGAG
    ATGGAAGATG ATGACTTTAC GGCCTCTCTG TCAAAGCAGA GTTGCATTAC TGAACAAACT
    CAGTATTTTT TCGATAACGA CAGTAAATCA TTTAGTGGTG TATTAGATTG TGGAAACTGT
    TCCAGAATCT TCCATGTAGA AAAACTTATG AACACCAACT TAATATTCAT AATGGTTGAG
    AGCAAAGGAA CTTGTCCCTG TGACACACGG TTGCTCATAC AAGCAGAGCA GACTTCTGAT
    GGTCCAGATC CTTGTGATAT GGTGAAGCAA CCCAGATACC GAAAAGGGCC TGATGTCTGC
    TTTGATAACA ATGCTTTGGA TCAACTTGTA TGCACAACGA ATCGTGCCTG TACCATGATG
    TCAGCTCCTA TCTTCATTTT TCTGCAAGTT TTCATGTGCT GTGGATGGTC AGTCACTTTC
    TCGAATTTTT CTTTGTTTTT GTGTTTGGCT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846251.1
    CDS484..3756
    Translation

    Target ORF information:

    RefSeq Version XM_024990483.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATATTCAGA AACTCTGAAA CCGGATAATT TTGAAGAGTC TGGCTATACG
    TTCATAGCAC CAAGAGATTA CTGCAATGAC CTTAAAATAT CAGATAATAA CACCGAATTT
    CTTTTAAATT TTAATGAGTT TATTGATAGA AAAACTCCAA ACAACCCATC ATGCAACACA
    GACTTGATTA ATAGAGTCTT GCTGGATGCA GGCTTTACAA ATGAACTTGT CCAAAATTAC
    TGGAGTAAGC AGAAAAACAT CAAGGGAGTG AAAGCACGAT TTGTTGTGAC TGATGGTGGG
    ATTACCAGAG TTTATCCGAA AGAGGCTGGA GAAAATTGGC AAGAAAACCC AGAAACATAT
    GAGGACAGCT TCTATAAAAG AAGTCTAGAT AACGATAACT ATGTTTTCAC TGCTCCCTAC
    TTTAACAAAA GTGGACCTGG TGCTTATGAA TCAGGCATTA TGGTAAGCAA AGCTGTAGAA
    ATTTACATCC AAGGAAAACT TCTTAAACCT GCAGTTGTTG GAATTAAAAT TGATCTAAAT
    TCCTGGATAG AGAATTTCAC CAAAACATCA ATCAGGGATC CGTGTGCGGG TCCAGTTTGT
    GACTGTAAAA GAAACAGTGA TGTAATGGAC TGTGTTATTC TAGATGATGG TGGGTTCCTT
    TTGATGGCAA ATCATGATGA TTATACTAAC CAGATTGGAA GATTTTTTGG AGAGATTGAT
    CCAAGTTTGA TGAGACACCT GGTTAATATA TCAGTTTATG CTTATAACAA GTCTTATGAT
    TATCAGTCGG TGTGTGAGCC AGGAGCTGCA CCAAAACAAG GAGCAGGGCA TCGCTCAGCA
    TACGTGCCAT CAATAGCCGA CGTATTACAC ATTGGCTGGT GGGCCACTGC TGCTGCCTGG
    TCTATTCTAC AGCAGTTTCT CTTGAGTTTG ACCTTTCCAC GACTTCTTGA GGCAGTTGAG
    ATGGAAGATG ATGACTTTAC GGCCTCTCTG TCAAAGCAGA GTTGCATTAC TGAACAAACT
    CAGTATTTTT TCGATAACGA CAGTAAATCA TTTAGTGGTG TATTAGATTG TGGAAACTGT
    TCCAGAATCT TCCATGTAGA AAAACTTATG AACACCAACT TAATATTCAT AATGGTTGAG
    AGCAAAGGAA CTTGTCCCTG TGACACACGG TTGCTCATAC AAGCAGAGCA GACTTCTGAT
    GGTCCAGATC CTTGTGATAT GGTGAAGCAA CCCAGATACC GAAAAGGGCC TGATGTCTGC
    TTTGATAACA ATGCTTTGGA TCAACTTGTA TGCACAACGA ATCGTGCCTG TACCATGATG
    TCAGCTCCTA TCTTCATTTT TCTGCAAGTT TTCATGTGCT GTGGATGGTC AGTCACTTTC
    TCGAATTTTT CTTTGTTTTT GTGTTTGGCT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233547
    Clone ID Related Accession (Same CDS sequence) XM_024990484.1
    Accession Version XM_024990484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG GAGCCAGTAA CTTTGGATTT CCTTGATGCA
    GAATTAGAGA ACGATATTAA AGTGGAGATT CGAAATAAAA TGATAGATGG AGAAAGCGGA
    GAAAAAACAT TCAGGACTCT GGTTAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAC
    AGGACATATA CATGGACTCC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTACCA
    ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATCC
    AAAAAGGGCA GAATGAAGGA TTCAGAAACT CTGAAACCGG ATAATTTTGA AGAGTCTGGC
    TATACGTTCA TAGCACCAAG AGATTACTGC AATGACCTTA AAATATCAGA TAATAACACC
    GAATTTCTTT TAAATTTTAA TGAGTTTATT GATAGAAAAA CTCCAAACAA CCCATCATGC
    AACACAGACT TGATTAATAG AGTCTTGCTG GATGCAGGCT TTACAAATGA ACTTGTCCAA
    AATTACTGGA GTAAGCAGAA AAACATCAAG GGAGTGAAAG CACGATTTGT TGTGACTGAT
    GGTGGGATTA CCAGAGTTTA TCCGAAAGAG GCTGGAGAAA ATTGGCAAGA AAACCCAGAA
    ACATATGAGG ACAGCTTCTA TAAAAGAAGT CTAGATAACG ATAACTATGT TTTCACTGCT
    CCCTACTTTA ACAAAAGTGG ACCTGGTGCT TATGAATCAG GCATTATGGT AAGCAAAGCT
    GTAGAAATTT ACATCCAAGG AAAACTTCTT AAACCTGCAG TTGTTGGAAT TAAAATTGAT
    CTAAATTCCT GGATAGAGAA TTTCACCAAA ACATCAATCA GGGATCCGTG TGCGGGTCCA
    GTTTGTGACT GTAAAAGAAA CAGTGATGTA ATGGACTGTG TTATTCTAGA TGATGGTGGG
    TTCCTTTTGA TGGCAAATCA TGATGATTAT ACTAACCAGA TTGGAAGATT TTTTGGAGAG
    ATTGATCCAA GTTTGATGAG ACACCTGGTT AATATATCAG TTTATGCTTA TAACAAGTCT
    TATGATTATC AGTCGGTGTG TGAGCCAGGA GCTGCACCAA AACAAGGAGC AGGGCATCGC
    TCAGCATACG TGCCATCAAT AGCCGACGTA TTACACATTG GCTGGTGGGC CACTGCTGCT
    GCCTGGTCTA TTCTACAGCA GTTTCTCTTG AGTTTGACCT TTCCACGACT TCTTGAGGCA
    GTTGAGATGG AAGATGATGA CTTTACGGCC TCTCTGTCAA AGCAGAGTTG CATTACTGAA
    CAAACTCAGT ATTTTTTCGA TAACGACAGT AAATCATTTA GTGGTGTATT AGATTGTGGA
    AACTGTTCCA GAATCTTCCA TGTAGAAAAA CTTATGAACA CCAACTTAAT ATTCATAATG
    GTTGAGAGCA AAGGAACTTG TCCCTGTGAC ACACGGTTGC TCATACAAGC AGAGCAGACT
    TCTGATGGTC CAGATCCTTG TGATATGGTG AAGCAACCCA GATACCGAAA AGGGCCTGAT
    GTCTGCTTTG ATAACAATGC TTTGGAGGAC TATACCGACT GTGGTGGTGT CTCTGGATTA
    AACCCCTCCC TGTGGTCCAT CATCGGCATC CAGTGTGTGC TACTTTGGCT CTTATCTGGC
    AGCAGACACC ACCAGTTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846252.1
    CDS484..3744
    Translation

    Target ORF information:

    RefSeq Version XM_024990484.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG GAGCCAGTAA CTTTGGATTT CCTTGATGCA
    GAATTAGAGA ACGATATTAA AGTGGAGATT CGAAATAAAA TGATAGATGG AGAAAGCGGA
    GAAAAAACAT TCAGGACTCT GGTTAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAC
    AGGACATATA CATGGACTCC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTACCA
    ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATCC
    AAAAAGGGCA GAATGAAGGA TTCAGAAACT CTGAAACCGG ATAATTTTGA AGAGTCTGGC
    TATACGTTCA TAGCACCAAG AGATTACTGC AATGACCTTA AAATATCAGA TAATAACACC
    GAATTTCTTT TAAATTTTAA TGAGTTTATT GATAGAAAAA CTCCAAACAA CCCATCATGC
    AACACAGACT TGATTAATAG AGTCTTGCTG GATGCAGGCT TTACAAATGA ACTTGTCCAA
    AATTACTGGA GTAAGCAGAA AAACATCAAG GGAGTGAAAG CACGATTTGT TGTGACTGAT
    GGTGGGATTA CCAGAGTTTA TCCGAAAGAG GCTGGAGAAA ATTGGCAAGA AAACCCAGAA
    ACATATGAGG ACAGCTTCTA TAAAAGAAGT CTAGATAACG ATAACTATGT TTTCACTGCT
    CCCTACTTTA ACAAAAGTGG ACCTGGTGCT TATGAATCAG GCATTATGGT AAGCAAAGCT
    GTAGAAATTT ACATCCAAGG AAAACTTCTT AAACCTGCAG TTGTTGGAAT TAAAATTGAT
    CTAAATTCCT GGATAGAGAA TTTCACCAAA ACATCAATCA GGGATCCGTG TGCGGGTCCA
    GTTTGTGACT GTAAAAGAAA CAGTGATGTA ATGGACTGTG TTATTCTAGA TGATGGTGGG
    TTCCTTTTGA TGGCAAATCA TGATGATTAT ACTAACCAGA TTGGAAGATT TTTTGGAGAG
    ATTGATCCAA GTTTGATGAG ACACCTGGTT AATATATCAG TTTATGCTTA TAACAAGTCT
    TATGATTATC AGTCGGTGTG TGAGCCAGGA GCTGCACCAA AACAAGGAGC AGGGCATCGC
    TCAGCATACG TGCCATCAAT AGCCGACGTA TTACACATTG GCTGGTGGGC CACTGCTGCT
    GCCTGGTCTA TTCTACAGCA GTTTCTCTTG AGTTTGACCT TTCCACGACT TCTTGAGGCA
    GTTGAGATGG AAGATGATGA CTTTACGGCC TCTCTGTCAA AGCAGAGTTG CATTACTGAA
    CAAACTCAGT ATTTTTTCGA TAACGACAGT AAATCATTTA GTGGTGTATT AGATTGTGGA
    AACTGTTCCA GAATCTTCCA TGTAGAAAAA CTTATGAACA CCAACTTAAT ATTCATAATG
    GTTGAGAGCA AAGGAACTTG TCCCTGTGAC ACACGGTTGC TCATACAAGC AGAGCAGACT
    TCTGATGGTC CAGATCCTTG TGATATGGTG AAGCAACCCA GATACCGAAA AGGGCCTGAT
    GTCTGCTTTG ATAACAATGC TTTGGAGGAC TATACCGACT GTGGTGGTGT CTCTGGATTA
    AACCCCTCCC TGTGGTCCAT CATCGGCATC CAGTGTGTGC TACTTTGGCT CTTATCTGGC
    AGCAGACACC ACCAGTTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233548
    Clone ID Related Accession (Same CDS sequence) XM_024990485.1
    Accession Version XM_024990485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATATTCAGA AACTCTGAAA CCGGATAATT TTGAAGAGTC TGGCTATACG
    TTCATAGCAC CAAGAGATTA CTGCAATGAC CTTAAAATAT CAGATAATAA CACCGAATTT
    CTTTTAAATT TTAATGAGTT TATTGATAGA AAAACTCCAA ACAACCCATC ATGCAACACA
    GACTTGATTA ATAGAGTCTT GCTGGATGCA GGCTTTACAA ATGAACTTGT CCAAAATTAC
    TGGAGTAAGC AGAAAAACAT CAAGGGAGTG AAAGCACGAT TTGTTGTGAC TGATGGTGGG
    ATTACCAGAG TTTATCCGAA AGAGGCTGGA GAAAATTGGC AAGAAAACCC AGAAACATAT
    GAGGACAGCT TCTATAAAAG AAGTCTAGAT AACGATAACT ATGTTTTCAC TGCTCCCTAC
    TTTAACAAAA GTGGACCTGG TGCTTATGAA TCAGGCATTA TGGTAAGCAA AGCTGTAGAA
    ATTTACATCC AAGGAAAACT TCTTAAACCT GCAGTTGTTG GAATTAAAAT TGATCTAAAT
    TCCTGGATAG AGAATTTCAC CAAAACATCA ATCAGGGATC CGTGTGCGGG TCCAGTTTGT
    GACTGTAAAA GAAACAGTGA TGTAATGGAC TGTGTTATTC TAGATGATGG TGGGTTCCTT
    TTGATGGCAA ATCATGATGA TTATACTAAC CAGATTGGAA GATTTTTTGG AGAGATTGAT
    CCAAGTTTGA TGAGACACCT GGTTAATATA TCAGTTTATG CTTATAACAA GTCTTATGAT
    TATCAGTCGG TGTGTGAGCC AGGAGCTGCA CCAAAACAAG GAGCAGGGCA TCGCTCAGCA
    TACGTGCCAT CAATAGCCGA CGTATTACAC ATTGGCTGGT GGGCCACTGC TGCTGCCTGG
    TCTATTCTAC AGCAGTTTCT CTTGAGTTTG ACCTTTCCAC GACTTCTTGA GGCAGTTGAG
    ATGGAAGATG ATGACTTTAC GGCCTCTCTG TCAAAGCAGA GTTGCATTAC TGAACAAACT
    CAGTATTTTT TCGATAACGA CAGTAAATCA TTTAGTGGTG TATTAGATTG TGGAAACTGT
    TCCAGAATCT TCCATGTAGA AAAACTTATG AACACCAACT TAATATTCAT AATGGTTGAG
    AGCAAAGGAA CTTGTCCCTG TGACACACGG TTGCTCATAC AAGCAGAGCA GACTTCTGAT
    GGTCCAGATC CTTGTGATAT GGTGAAGCAA CCCAGATACC GAAAAGGGCC TGATGTCTGC
    TTTGATAACA ATGCTTTGGA GGACTATACC GACTGTGGTG GTGTCTCTGG ATTAAACCCC
    TCCCTGTGGT CCATCATCGG CATCCAGTGT GTGCTACTTT GGCTCTTATC TGGCAGCAGA
    CACCACCAGT TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846253.1
    CDS484..3738
    Translation

    Target ORF information:

    RefSeq Version XM_024990485.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATATTCAGA AACTCTGAAA CCGGATAATT TTGAAGAGTC TGGCTATACG
    TTCATAGCAC CAAGAGATTA CTGCAATGAC CTTAAAATAT CAGATAATAA CACCGAATTT
    CTTTTAAATT TTAATGAGTT TATTGATAGA AAAACTCCAA ACAACCCATC ATGCAACACA
    GACTTGATTA ATAGAGTCTT GCTGGATGCA GGCTTTACAA ATGAACTTGT CCAAAATTAC
    TGGAGTAAGC AGAAAAACAT CAAGGGAGTG AAAGCACGAT TTGTTGTGAC TGATGGTGGG
    ATTACCAGAG TTTATCCGAA AGAGGCTGGA GAAAATTGGC AAGAAAACCC AGAAACATAT
    GAGGACAGCT TCTATAAAAG AAGTCTAGAT AACGATAACT ATGTTTTCAC TGCTCCCTAC
    TTTAACAAAA GTGGACCTGG TGCTTATGAA TCAGGCATTA TGGTAAGCAA AGCTGTAGAA
    ATTTACATCC AAGGAAAACT TCTTAAACCT GCAGTTGTTG GAATTAAAAT TGATCTAAAT
    TCCTGGATAG AGAATTTCAC CAAAACATCA ATCAGGGATC CGTGTGCGGG TCCAGTTTGT
    GACTGTAAAA GAAACAGTGA TGTAATGGAC TGTGTTATTC TAGATGATGG TGGGTTCCTT
    TTGATGGCAA ATCATGATGA TTATACTAAC CAGATTGGAA GATTTTTTGG AGAGATTGAT
    CCAAGTTTGA TGAGACACCT GGTTAATATA TCAGTTTATG CTTATAACAA GTCTTATGAT
    TATCAGTCGG TGTGTGAGCC AGGAGCTGCA CCAAAACAAG GAGCAGGGCA TCGCTCAGCA
    TACGTGCCAT CAATAGCCGA CGTATTACAC ATTGGCTGGT GGGCCACTGC TGCTGCCTGG
    TCTATTCTAC AGCAGTTTCT CTTGAGTTTG ACCTTTCCAC GACTTCTTGA GGCAGTTGAG
    ATGGAAGATG ATGACTTTAC GGCCTCTCTG TCAAAGCAGA GTTGCATTAC TGAACAAACT
    CAGTATTTTT TCGATAACGA CAGTAAATCA TTTAGTGGTG TATTAGATTG TGGAAACTGT
    TCCAGAATCT TCCATGTAGA AAAACTTATG AACACCAACT TAATATTCAT AATGGTTGAG
    AGCAAAGGAA CTTGTCCCTG TGACACACGG TTGCTCATAC AAGCAGAGCA GACTTCTGAT
    GGTCCAGATC CTTGTGATAT GGTGAAGCAA CCCAGATACC GAAAAGGGCC TGATGTCTGC
    TTTGATAACA ATGCTTTGGA GGACTATACC GACTGTGGTG GTGTCTCTGG ATTAAACCCC
    TCCCTGTGGT CCATCATCGG CATCCAGTGT GTGCTACTTT GGCTCTTATC TGGCAGCAGA
    CACCACCAGT TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233549
    Clone ID Related Accession (Same CDS sequence) XM_024990486.1
    Accession Version XM_024990486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG GAGCCAGTAA CTTTGGATTT CCTTGATGCA
    GAATTAGAGA ACGATATTAA AGTGGAGATT CGAAATAAAA TGATAGATGG AGAAAGCGGA
    GAAAAAACAT TCAGGACTCT GGTTAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAC
    AGGACATATA CATGGACTCC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTACCA
    ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATAT
    TCAGAAACTC TGAAACCGGA TAATTTTGAA GAGTCTGGCT ATACGTTCAT AGCACCAAGA
    GATTACTGCA ATGACCTTAA AATATCAGAT AATAACACCG AATTTCTTTT AAATTTTAAT
    GAGTTTATTG ATAGAAAAAC TCCAAACAAC CCATCATGCA ACACAGACTT GATTAATAGA
    GTCTTGCTGG ATGCAGGCTT TACAAATGAA CTTGTCCAAA ATTACTGGAG TAAGCAGAAA
    AACATCAAGG GAGTGAAAGC ACGATTTGTT GTGACTGATG GTGGGATTAC CAGAGTTTAT
    CCGAAAGAGG CTGGAGAAAA TTGGCAAGAA AACCCAGAAA CATATGAGGA CAGCTTCTAT
    AAAAGAAGTC TAGATAACGA TAACTATGTT TTCACTGCTC CCTACTTTAA CAAAAGTGGA
    CCTGGTGCTT ATGAATCAGG CATTATGGTA AGCAAAGCTG TAGAAATTTA CATCCAAGGA
    AAACTTCTTA AACCTGCAGT TGTTGGAATT AAAATTGATC TAAATTCCTG GATAGAGAAT
    TTCACCAAAA CATCAATCAG GGATCCGTGT GCGGGTCCAG TTTGTGACTG TAAAAGAAAC
    AGTGATGTAA TGGACTGTGT TATTCTAGAT GATGGTGGGT TCCTTTTGAT GGCAAATCAT
    GATGATTATA CTAACCAGAT TGGAAGATTT TTTGGAGAGA TTGATCCAAG TTTGATGAGA
    CACCTGGTTA ATATATCAGT TTATGCTTAT AACAAGTCTT ATGATTATCA GTCGGTGTGT
    GAGCCAGGAG CTGCACCAAA ACAAGGAGCA GGGCATCGCT CAGCATACGT GCCATCAATA
    GCCGACGTAT TACACATTGG CTGGTGGGCC ACTGCTGCTG CCTGGTCTAT TCTACAGCAG
    TTTCTCTTGA GTTTGACCTT TCCACGACTT CTTGAGGCAG TTGAGATGGA AGATGATGAC
    TTTACGGCCT CTCTGTCAAA GCAGAGTTGC ATTACTGAAC AAACTCAGTA TTTTTTCGAT
    AACGACAGTA AATCATTTAG TGGTGTATTA GATTGTGGAA ACTGTTCCAG AATCTTCCAT
    GTAGAAAAAC TTATGAACAC CAACTTAATA TTCATAATGG TTGAGAGCAA AGGAACTTGT
    CCCTGTGACA CACGGTTGCT CATACAAGCA GAGCAGACTT CTGATGGTCC AGATCCTTGT
    GATATGGTGA AGCAACCCAG ATACCGAAAA GGGCCTGATG TCTGCTTTGA TAACAATGCT
    TTGGAGGACT ATACCGACTG TGGTGGTGTC TCTGGATTAA ACCCCTCCCT GTGGTCCATC
    ATCGGCATCC AGTGTGTGCT ACTTTGGCTC TTATCTGGCA GCAGACACCA CCAGTTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846254.1
    CDS484..3723
    Translation

    Target ORF information:

    RefSeq Version XM_024990486.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG GAGCCAGTAA CTTTGGATTT CCTTGATGCA
    GAATTAGAGA ACGATATTAA AGTGGAGATT CGAAATAAAA TGATAGATGG AGAAAGCGGA
    GAAAAAACAT TCAGGACTCT GGTTAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAC
    AGGACATATA CATGGACTCC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTACCA
    ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATAT
    TCAGAAACTC TGAAACCGGA TAATTTTGAA GAGTCTGGCT ATACGTTCAT AGCACCAAGA
    GATTACTGCA ATGACCTTAA AATATCAGAT AATAACACCG AATTTCTTTT AAATTTTAAT
    GAGTTTATTG ATAGAAAAAC TCCAAACAAC CCATCATGCA ACACAGACTT GATTAATAGA
    GTCTTGCTGG ATGCAGGCTT TACAAATGAA CTTGTCCAAA ATTACTGGAG TAAGCAGAAA
    AACATCAAGG GAGTGAAAGC ACGATTTGTT GTGACTGATG GTGGGATTAC CAGAGTTTAT
    CCGAAAGAGG CTGGAGAAAA TTGGCAAGAA AACCCAGAAA CATATGAGGA CAGCTTCTAT
    AAAAGAAGTC TAGATAACGA TAACTATGTT TTCACTGCTC CCTACTTTAA CAAAAGTGGA
    CCTGGTGCTT ATGAATCAGG CATTATGGTA AGCAAAGCTG TAGAAATTTA CATCCAAGGA
    AAACTTCTTA AACCTGCAGT TGTTGGAATT AAAATTGATC TAAATTCCTG GATAGAGAAT
    TTCACCAAAA CATCAATCAG GGATCCGTGT GCGGGTCCAG TTTGTGACTG TAAAAGAAAC
    AGTGATGTAA TGGACTGTGT TATTCTAGAT GATGGTGGGT TCCTTTTGAT GGCAAATCAT
    GATGATTATA CTAACCAGAT TGGAAGATTT TTTGGAGAGA TTGATCCAAG TTTGATGAGA
    CACCTGGTTA ATATATCAGT TTATGCTTAT AACAAGTCTT ATGATTATCA GTCGGTGTGT
    GAGCCAGGAG CTGCACCAAA ACAAGGAGCA GGGCATCGCT CAGCATACGT GCCATCAATA
    GCCGACGTAT TACACATTGG CTGGTGGGCC ACTGCTGCTG CCTGGTCTAT TCTACAGCAG
    TTTCTCTTGA GTTTGACCTT TCCACGACTT CTTGAGGCAG TTGAGATGGA AGATGATGAC
    TTTACGGCCT CTCTGTCAAA GCAGAGTTGC ATTACTGAAC AAACTCAGTA TTTTTTCGAT
    AACGACAGTA AATCATTTAG TGGTGTATTA GATTGTGGAA ACTGTTCCAG AATCTTCCAT
    GTAGAAAAAC TTATGAACAC CAACTTAATA TTCATAATGG TTGAGAGCAA AGGAACTTGT
    CCCTGTGACA CACGGTTGCT CATACAAGCA GAGCAGACTT CTGATGGTCC AGATCCTTGT
    GATATGGTGA AGCAACCCAG ATACCGAAAA GGGCCTGATG TCTGCTTTGA TAACAATGCT
    TTGGAGGACT ATACCGACTG TGGTGGTGTC TCTGGATTAA ACCCCTCCCT GTGGTCCATC
    ATCGGCATCC AGTGTGTGCT ACTTTGGCTC TTATCTGGCA GCAGACACCA CCAGTTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233550
    Clone ID Related Accession (Same CDS sequence) XM_024990487.1
    Accession Version XM_024990487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCAAGAAG ACCTTGTCAC CCTGGCAAAA ACAGCAAGTG GAGTCAATCA GCTTGTCGAT 
    ATTTATGAGA AATACCAAGA TTTGTATACT GTGGAGCCAA ATAATGCACG TCAGCTGGTG
    GAAATTGCAG CCAGGGATAT TGAAAAACTT CTGAGCAACA GATCCAAAGC CCTGGTGCGT
    CTAGCTTTGG AAGCAGAGAA AGTTCAAGCA GCCCACCAGT GGAGAGAGGA TTTCGCAAGC
    AATGAAGTTG TGTACTACAA TGCAAAGGAT GATCTTGATC CAGAAAAGAA TGATAGTGAA
    CCAGGCAGCC AGAGGATAAA ACCTGTTTTT ATCGATGATG CTAATTTTGG GCGACAAATA
    TCCTATCAGC ACGCAGCCGT TCATATTCCC ACTGACATCT ATGAGGGCTC AACAATTGTA
    TTAAATGAAC TCAACTGGAC AAGTGCCTTA GATGAAGTTT TCAAGAAAAA TCGAGAAGAA
    GATCCTTCAT TATTGTGGCA GGTGTTTGGC AGTGCCACAG GCCTGGCCCG ATATTATCCA
    GCTTCTCCAT GGGTTGATAA CAGTAGAACT CCAAACAAAA TTGACCTTTA TGATGTACGC
    AGGAGACCAT GGTACATCCA AGGAGCTGCA TCTCCTAAAG ACATGCTTAT TCTGGTTGAT
    GTGAGTGGGA GTGTTAGTGG TTTGACACTT AAACTGATCC GAACATCTGT CTCCGAAATG
    TTGGAAACCC TCTCAGATGA CGATTTTGTG AATGTAGCTT CATTTAACAG CAATGCCCAG
    GATGTAAGCT GTTTTCAGCA CCTTGTCCAA GCAAATGTGA GAAATAAGAA AGTGCTGAAA
    GATGCAGTGA ATAATATCAC AGCAAAAGGA ATCACAGATT ATAAGAAGGG ATTTAGTTTT
    GCTTTTGAAC AGCTGCTTAA TTATAATGTG TCTAGAGCCA ACTGCAATAA GATTATCATG
    TTGTTCACGG ATGGAGGAGA AGAGAGAGCT CAGGAGATAT TTACCAAATA CAACAAAGAC
    AAAAAGGTCC GTGTATTCAC ATTTTCAGTT GGTCAACATA ATTATGACAG AGGACCTATT
    CAGTGGATGG CCTGTGAAAA TAAAGGTTAT TATTACGAAA TTCCTTCCAT TGGAGCAATA
    AGAATCAATA CTCAGGAATA TTTGGATGTT CTGGGAAGAC CAATGGTTTT AGCTGGAGAC
    AAAGCTAAGC AAGTCCAGTG GACAAACGTA TACCTGGATG CACTGGAACT GGGACTTGTC
    ATTACTGGAA CTCTTCCGGT CTTCAACATA ACCGGCCAAA ATGAAAATAA GACAAACTTA
    AAGAATCAGC TGATTCTTGG TGTGATGGGA GTTGATGTGT CCTTGGAAGA TATTAAAAGA
    CTAACACCAC GTTTTACATT GTGCCCCAAT GGCTATTATT TTGCAATTGA TCCTAATGGT
    TATGTTTTAT TACATCCAAA TCTTCAGCCA AAGCCTATTG GCTTAGGTAT ACCAACAATT
    AATTTAAGAA AAAGGAGACC CAATGTTCAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAACG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG ATCAACTTGT ATGCACAACG
    AATCGTGCCT GTACCATGAT GTCAGCTCCT ATCTTCATTT TTCTGCAAGT TTTCATGTGC
    TGTGGATGGT CAGTCACTTT CTCGAATTTT TCTTTGTTTT TGTGTTTGGC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846255.1
    CDS161..3394
    Translation

    Target ORF information:

    RefSeq Version XM_024990487.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCAAGAAG ACCTTGTCAC CCTGGCAAAA ACAGCAAGTG GAGTCAATCA GCTTGTCGAT 
    ATTTATGAGA AATACCAAGA TTTGTATACT GTGGAGCCAA ATAATGCACG TCAGCTGGTG
    GAAATTGCAG CCAGGGATAT TGAAAAACTT CTGAGCAACA GATCCAAAGC CCTGGTGCGT
    CTAGCTTTGG AAGCAGAGAA AGTTCAAGCA GCCCACCAGT GGAGAGAGGA TTTCGCAAGC
    AATGAAGTTG TGTACTACAA TGCAAAGGAT GATCTTGATC CAGAAAAGAA TGATAGTGAA
    CCAGGCAGCC AGAGGATAAA ACCTGTTTTT ATCGATGATG CTAATTTTGG GCGACAAATA
    TCCTATCAGC ACGCAGCCGT TCATATTCCC ACTGACATCT ATGAGGGCTC AACAATTGTA
    TTAAATGAAC TCAACTGGAC AAGTGCCTTA GATGAAGTTT TCAAGAAAAA TCGAGAAGAA
    GATCCTTCAT TATTGTGGCA GGTGTTTGGC AGTGCCACAG GCCTGGCCCG ATATTATCCA
    GCTTCTCCAT GGGTTGATAA CAGTAGAACT CCAAACAAAA TTGACCTTTA TGATGTACGC
    AGGAGACCAT GGTACATCCA AGGAGCTGCA TCTCCTAAAG ACATGCTTAT TCTGGTTGAT
    GTGAGTGGGA GTGTTAGTGG TTTGACACTT AAACTGATCC GAACATCTGT CTCCGAAATG
    TTGGAAACCC TCTCAGATGA CGATTTTGTG AATGTAGCTT CATTTAACAG CAATGCCCAG
    GATGTAAGCT GTTTTCAGCA CCTTGTCCAA GCAAATGTGA GAAATAAGAA AGTGCTGAAA
    GATGCAGTGA ATAATATCAC AGCAAAAGGA ATCACAGATT ATAAGAAGGG ATTTAGTTTT
    GCTTTTGAAC AGCTGCTTAA TTATAATGTG TCTAGAGCCA ACTGCAATAA GATTATCATG
    TTGTTCACGG ATGGAGGAGA AGAGAGAGCT CAGGAGATAT TTACCAAATA CAACAAAGAC
    AAAAAGGTCC GTGTATTCAC ATTTTCAGTT GGTCAACATA ATTATGACAG AGGACCTATT
    CAGTGGATGG CCTGTGAAAA TAAAGGTTAT TATTACGAAA TTCCTTCCAT TGGAGCAATA
    AGAATCAATA CTCAGGAATA TTTGGATGTT CTGGGAAGAC CAATGGTTTT AGCTGGAGAC
    AAAGCTAAGC AAGTCCAGTG GACAAACGTA TACCTGGATG CACTGGAACT GGGACTTGTC
    ATTACTGGAA CTCTTCCGGT CTTCAACATA ACCGGCCAAA ATGAAAATAA GACAAACTTA
    AAGAATCAGC TGATTCTTGG TGTGATGGGA GTTGATGTGT CCTTGGAAGA TATTAAAAGA
    CTAACACCAC GTTTTACATT GTGCCCCAAT GGCTATTATT TTGCAATTGA TCCTAATGGT
    TATGTTTTAT TACATCCAAA TCTTCAGCCA AAGCCTATTG GCTTAGGTAT ACCAACAATT
    AATTTAAGAA AAAGGAGACC CAATGTTCAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAACG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG ATCAACTTGT ATGCACAACG
    AATCGTGCCT GTACCATGAT GTCAGCTCCT ATCTTCATTT TTCTGCAAGT TTTCATGTGC
    TGTGGATGGT CAGTCACTTT CTCGAATTTT TCTTTGTTTT TGTGTTTGGC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38532
    Clone ID Related Accession (Same CDS sequence) XM_010804141.2 , NM_001205987.1
    Accession Version NM_001205987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-22
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03056565.1, AAFC03006352.1, AAFC03006350.1, AAFC03006737.1, AAFC03069160.1, AAFC03013689.1, AAFC03013690.1, AAFC03013692.1, AAFC03013691.1 and AAFC03013693.1. On Apr 21, 2011 this sequence version replaced XM_609993.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01093727, SAMN01093728 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAATG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG AGGACTATAC CGACTGTGGT
    GGTGTCTCTG GATTAAACCC CTCCCTGTGG TCCATCATCG GCATCCAGTG TGTGCTACTT
    TGGCTCTTAT CTGGCAGCAG ACACCACCAG TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192916.1
    CDS22..3297
    Translation

    Target ORF information:

    RefSeq Version NM_001205987.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAATG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG AGGACTATAC CGACTGTGGT
    GGTGTCTCTG GATTAAACCC CTCCCTGTGG TCCATCATCG GCATCCAGTG TGTGCTACTT
    TGGCTCTTAT CTGGCAGCAG ACACCACCAG TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38532
    Clone ID Related Accession (Same CDS sequence) XM_010804141.2 , NM_001205987.1
    Accession Version XM_010804141.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103890.1) and transcript sequence (NM_001205987.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 38 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAATG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG AGGACTATAC CGACTGTGGT
    GGTGTCTCTG GATTAAACCC CTCCCTGTGG TCCATCATCG GCATCCAGTG TGTGCTACTT
    TGGCTCTTAT CTGGCAGCAG ACACCACCAG TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802443.1
    CDS412..3687
    Translation

    Target ORF information:

    RefSeq Version XM_010804141.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804141.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTT GTTGATCGGC 
    CCCTCGTCGG AGGAGCCGTT CCCTTCGGCC GTCACTATCA AGTCATGGGT GGATAAGATG
    CAAGAAGACC TTGTCACCCT GGCAAAAACA GCAAGTGGAG TCAATCAGCT TGTCGATATT
    TATGAGAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGTCA GCTGGTGGAA
    ATTGCAGCCA GGGATATTGA AAAACTTCTG AGCAACAGAT CCAAAGCCCT GGTGCGTCTA
    GCTTTGGAAG CAGAGAAAGT TCAAGCAGCC CACCAGTGGA GAGAGGATTT CGCAAGCAAT
    GAAGTTGTGT ACTACAATGC AAAGGATGAT CTTGATCCAG AAAAGAATGA TAGTGAACCA
    GGCAGCCAGA GGATAAAACC TGTTTTTATC GATGATGCTA ATTTTGGGCG ACAAATATCC
    TATCAGCACG CAGCCGTTCA TATTCCCACT GACATCTATG AGGGCTCAAC AATTGTATTA
    AATGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AGAAAAATCG AGAAGAAGAT
    CCTTCATTAT TGTGGCAGGT GTTTGGCAGT GCCACAGGCC TGGCCCGATA TTATCCAGCT
    TCTCCATGGG TTGATAACAG TAGAACTCCA AACAAAATTG ACCTTTATGA TGTACGCAGG
    AGACCATGGT ACATCCAAGG AGCTGCATCT CCTAAAGACA TGCTTATTCT GGTTGATGTG
    AGTGGGAGTG TTAGTGGTTT GACACTTAAA CTGATCCGAA CATCTGTCTC CGAAATGTTG
    GAAACCCTCT CAGATGACGA TTTTGTGAAT GTAGCTTCAT TTAACAGCAA TGCCCAGGAT
    GTAAGCTGTT TTCAGCACCT TGTCCAAGCA AATGTGAGAA ATAAGAAAGT GCTGAAAGAT
    GCAGTGAATA ATATCACAGC AAAAGGAATC ACAGATTATA AGAAGGGATT TAGTTTTGCT
    TTTGAACAGC TGCTTAATTA TAATGTGTCT AGAGCCAACT GCAATAAGAT TATCATGTTG
    TTCACGGATG GAGGAGAAGA GAGAGCTCAG GAGATATTTA CCAAATACAA CAAAGACAAA
    AAGGTCCGTG TATTCACATT TTCAGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG
    TGGATGGCCT GTGAAAATAA AGGTTATTAT TACGAAATTC CTTCCATTGG AGCAATAAGA
    ATCAATACTC AGGAATATTT GGATGTTCTG GGAAGACCAA TGGTTTTAGC TGGAGACAAA
    GCTAAGCAAG TCCAGTGGAC AAACGTATAC CTGGATGCAC TGGAACTGGG ACTTGTCATT
    ACTGGAACTC TTCCGGTCTT CAACATAACC GGCCAAAATG AAAATAAGAC AAACTTAAAG
    AATCAGCTGA TTCTTGGTGT GATGGGAGTT GATGTGTCCT TGGAAGATAT TAAAAGACTA
    ACACCACGTT TTACATTGTG CCCCAATGGC TATTATTTTG CAATTGATCC TAATGGTTAT
    GTTTTATTAC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC AGTAACTTTG
    GATTTCCTTG ATGCAGAATT AGAGAACGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA
    GATGGAGAAA GCGGAGAAAA AACATTCAGG ACTCTGGTTA AATCTCAAGA TGAGAGATAT
    ATTGACAAAG GAAACAGGAC ATATACATGG ACTCCTGTCA ATGGCACAGA TTACAGTTTG
    GCCTTGGTAT TACCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA
    ACTCAGGCCA GATCCAAAAA GGGCAGAATG AAGGATTCAG AAACTCTGAA ACCGGATAAT
    TTTGAAGAGT CTGGCTATAC GTTCATAGCA CCAAGAGATT ACTGCAATGA CCTTAAAATA
    TCAGATAATA ACACCGAATT TCTTTTAAAT TTTAATGAGT TTATTGATAG AAAAACTCCA
    AACAACCCAT CATGCAACAC AGACTTGATT AATAGAGTCT TGCTGGATGC AGGCTTTACA
    AATGAACTTG TCCAAAATTA CTGGAGTAAG CAGAAAAACA TCAAGGGAGT GAAAGCACGA
    TTTGTTGTGA CTGATGGTGG GATTACCAGA GTTTATCCGA AAGAGGCTGG AGAAAATTGG
    CAAGAAAACC CAGAAACATA TGAGGACAGC TTCTATAAAA GAAGTCTAGA TAACGATAAC
    TATGTTTTCA CTGCTCCCTA CTTTAACAAA AGTGGACCTG GTGCTTATGA ATCAGGCATT
    ATGGTAAGCA AAGCTGTAGA AATTTACATC CAAGGAAAAC TTCTTAAACC TGCAGTTGTT
    GGAATTAAAA TTGATCTAAA TTCCTGGATA GAGAATTTCA CCAAAACATC AATCAGGGAT
    CCGTGTGCGG GTCCAGTTTG TGACTGTAAA AGAAACAGTG ATGTAATGGA CTGTGTTATT
    CTAGATGATG GTGGGTTCCT TTTGATGGCA AATCATGATG ATTATACTAA CCAGATTGGA
    AGATTTTTTG GAGAGATTGA TCCAAGTTTG ATGAGACACC TGGTTAATAT ATCAGTTTAT
    GCTTATAACA AGTCTTATGA TTATCAGTCG GTGTGTGAGC CAGGAGCTGC ACCAAAACAA
    GGAGCAGGGC ATCGCTCAGC ATACGTGCCA TCAATAGCCG ACGTATTACA CATTGGCTGG
    TGGGCCACTG CTGCTGCCTG GTCTATTCTA CAGCAGTTTC TCTTGAGTTT GACCTTTCCA
    CGACTTCTTG AGGCAGTTGA GATGGAAGAT GATGACTTTA CGGCCTCTCT GTCAAAGCAG
    AGTTGCATTA CTGAACAAAC TCAGTATTTT TTCGATAATG ACAGTAAATC ATTTAGTGGT
    GTATTAGATT GTGGAAACTG TTCCAGAATC TTCCATGTAG AAAAACTTAT GAACACCAAC
    TTAATATTCA TAATGGTTGA GAGCAAAGGA ACTTGTCCCT GTGACACACG GTTGCTCATA
    CAAGCAGAGC AGACTTCTGA TGGTCCAGAT CCTTGTGATA TGGTGAAGCA ACCCAGATAC
    CGAAAAGGGC CTGATGTCTG CTTTGATAAC AATGCTTTGG AGGACTATAC CGACTGTGGT
    GGTGTCTCTG GATTAAACCC CTCCCTGTGG TCCATCATCG GCATCCAGTG TGTGCTACTT
    TGGCTCTTAT CTGGCAGCAG ACACCACCAG TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.