Cacna2d1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna2d1
Entrez Gene ID	25399
Full Name	calcium voltage-gated channel auxiliary subunit alpha2delta 1
Synonyms	CCHLA2,Cacna2,DHSCCA
General protein information	Preferred Names calcium voltage-gated channel auxiliary subunit alpha2delta 1 Names voltage-dependent calcium channel subunit alpha-2/delta-1 Calcium channel subunit alpha 2 delta (dihydropyridine - sensitive L-type) calcium channel, voltage-dependent, alpha2/delta subunit 1 voltage-gated calcium channel subunit alpha-2/delta-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q12
Summary	subunit of voltage-gated calcium channels; contributes to the regulation of N- and/or L-type calcium channels [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012919.3	NP_037051.2	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform 1 precursor
NM_001110847.1	NP_001104317.1	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform 3 precursor
XM_006235981.2	XP_006236043.1	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1
XM_017592485.1	XP_017447974.1	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X2
XM_006235983.3	XP_006236045.1	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X4
NM_001110848.1	NP_001104318.1	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform 2 precursor
XM_006235982.3	XP_006236044.1	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA2D1	NP_000713.2
Bos taurus (cattle)	CACNA2D1	NP_001192916.1
Danio rerio (zebrafish)	cacna2d1a	NP_001038425.2
Pan troglodytes (chimpanzee)	CACNA2D1	XP_001160279.1
	CACNA2D1	XP_002803438.1
Canis lupus familiaris (dog)	CACNA2D1	XP_005631077.1
Mus musculus (house mouse)	Cacna2d1	NP_001104314.1
Rattus norvegicus (Norway rat)	Cacna2d1	NP_037051.2
Gallus gallus (chicken)	CACNA2D1	NP_001170793.1
Xenopus tropicalis (tropical clawed frog)	cacna2d1	NP_001090738.1

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of calcium channel alpha-2-delta 1 subunit in dorsal horn contributes to spinal cord injury-induced tactile allodynia.
Kusuyama K, Tachibana T, Yamanaka H, Okubo M, Yoshiya S, Noguchi K
The spine journal : official journal of the North American Spine Society(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results unveil a novel functional coupling between Parkin and the CaV2.2 channels
Title: Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels.

Proteolytic processing of alpha2delta then permits voltage-dependent activation of the channels, acting as a checkpoint allowing trafficking only of mature calcium channel complexes into neuronal processes.
Title: Proteolytic maturation of ?

Data suggest that Cacna2d1 Asn663 (and Asn348, Asn468, Asn812) contributes to protein stability of cardiac CaV1.2 channels; N-glycosylation of Cacna2d1 is essential for functional cardiac CaV1.2 channels.
Title: Identification of Glycosylation Sites Essential for Surface Expression of the CaV?2?1 Subunit and Modulation of the Cardiac CaV1.2 Channel Activity.

there was an increased Cavalpha2delta-1 expression in the dorsal horn and an ectopic growths-to-colon cross-sensitization in female rats with established endometriosis
Title: Upregulation of ???-1 Calcium Channel Subunit in the Spinal Cord Contributes to Pelvic Organ Cross-Sensitization in a Rat Model of Experimentally-Induced Endometriosis.

Data indicate that residues 26-230 in the alpha2delta-1 (alpha2delta-1) subunit sequence, termed R-domain (Rd), as the minimal sequence required to induce Cav current.
Title: The R-Domain: Identification of an N-terminal Region of the ?2?-1 Subunit Which is Necessary and Sufficient for its Effects on Cav2.2 Calcium Currents.

The following Cacna2d1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna2d1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01313	NM_012919.3 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
ORa60772	XM_017592485.1 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa00863	NM_001110847.1 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa00867	NM_001110848.1 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa26958	XM_006235981.2 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa26959	XM_006235982.3 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa26960	XM_006235983.3 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna2d1 ( NM_012919.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037051.2 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa01313
Clone ID Related Accession (Same CDS sequence)	NM_012919.3
Accession Version	NM_012919.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF286488.1. On Aug 31, 2012 this sequence version replaced NM_012919.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF286488.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTACTGC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC GGTCACACTG 
GATTTTCTCG ATGCTGAGTT AGAGAATGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA 
GACGGAGAAA GTGGAGAAAA AACATTCAGA ACTCTGGTCA AATCTCAAGA TGAGAGATAT 
ATTGACAAAG GAAATCGAAC ATACACATGG ACACCTGTCA ATGGCACAGA TTACAGTTTG 
GCCTTGGTAT TGCCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA 
ACTCAGGCCA GATCAAAAAA GGGGAAAATG AAGGATTCAG AAACCCTGAA GCCAGACAAT 
TTTGAAGAAT CTGGCTACAC TTTCATAGCA CCAAGGGAAT ACTGCAATGA CCTGAAACCT 
TCAGATAATA ACACTGAATT TCTTTTAAAT TTCAATGAAT TTATTGATAG GAAAACTCCA 
AACAACCCTT CCTGTAATAC AGATTTGATT AATAGAATCT TGCTGGATGC GGGTTTTACA 
AATGAACTTG TCCAAAATTA TTGGAGTAAA CAGAAAAATA TCAAAGGAGT GAAGGCACGC 
TTTGTGGTGA CTGATGGCGG AATTACAAGA GTTTACCCCA AAGAGGCTGG AGAAAACTGG 
CAAGAGAACC CAGAGACGTA TGAGGACAGC TTCTACAAAC GGAGCCTAGA TAACGATAAC 
TACGTTTTCA CTGCGCCCTA CTTCAACAAA AGTGGACCTG GTGCCTATGA ATCTGGAATT 
ATGGTAAGCA AAGCTGTAGA ACTGTACATC CAAGGAAAAC TTCTTAAGCC TGCAGTTGTG 
GGAATTAAAA TTGACGTAAA CTCCTGGATA GAAAATTTTA CCAAAACTTC AATCAGGGAT 
CCGTGTGCTG GTCCAGTTTG TGACTGCAAA AGAAACAGTG ATGTAATGGA CTGTGTCATT 
CTAGATGATG GTGGATTTCT TCTGATGGCC AATCATGATG ATTACACTAA TCAGATTGGA 
CGTTTTTTTG GAGAGATTGA CCCGAGCATG ATGAGACACC TGGTTAATAT ATCACTTTAT 
GCATTCAATA AATCATATGA CTATCAGTCT GTGTGTGATC CAGGGGCAGC ACCAAAGCAA 
GGAGCAGGAC ATCGCTCAGC CTATGTGCCA TCAATTACAG ACATACTCCA GATTGGCTGG 
TGGGCCACTG CTGCCGCCTG GTCTATTCTC CAGCAGCTAC TCTTGAGTTT GACATTTCCA 
CGGCTCCTTG AGGCAGTTGA AATGGAAGAG GATGACTTCA CAGCTTCCCT GTCTAAGCAG 
AGCTGCATCA CAGAACAAAC TCAGTACTTC TTCAAGAACG ATACTAAATC ATTCAGTGGT 
TTACTGGACT GTGGAAACTG TTCCAGGATC TTTCATGTTG AGAAGCTTAT GAACACCAAC 
CTAGTGTTCA TAATGGTGGA GAGCAAGGGA ACATGTCCGT GTGACACGCG GCTGCTCATG 
CAAGCAGAAC AGACTTCTGA TGGTCCAGAT CCTTGCGACA TGGTTAAGCA GCCCAGATAC 
CGAAAAGGAC CTGATGTCTG CTTTGATAAC AATGTGCTGG AGGATTATAC CGACTGTGGT 
GGTGTTTCTG GATTAAACCC TTCCTTATGG TCTATCTTTG GACTCCAATT TATACTCCTT 
TGGCTGGTAT CTGGCAGCAG ACACTATCTA TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037051.2
CDS	286..3561
Translation	MAAGCLLALTLTLFQSWLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESESGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKIEETITQARSKKGKMKDSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSITDILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLW

Target ORF information:

RefSeq Version	NM_012919.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012919.3

ORF Insert Sequence:

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTACTGC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC GGTCACACTG 
GATTTTCTCG ATGCTGAGTT AGAGAATGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA 
GACGGAGAAA GTGGAGAAAA AACATTCAGA ACTCTGGTCA AATCTCAAGA TGAGAGATAT 
ATTGACAAAG GAAATCGAAC ATACACATGG ACACCTGTCA ATGGCACAGA TTACAGTTTG 
GCCTTGGTAT TGCCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA 
ACTCAGGCCA GATCAAAAAA GGGGAAAATG AAGGATTCAG AAACCCTGAA GCCAGACAAT 
TTTGAAGAAT CTGGCTACAC TTTCATAGCA CCAAGGGAAT ACTGCAATGA CCTGAAACCT 
TCAGATAATA ACACTGAATT TCTTTTAAAT TTCAATGAAT TTATTGATAG GAAAACTCCA 
AACAACCCTT CCTGTAATAC AGATTTGATT AATAGAATCT TGCTGGATGC GGGTTTTACA 
AATGAACTTG TCCAAAATTA TTGGAGTAAA CAGAAAAATA TCAAAGGAGT GAAGGCACGC 
TTTGTGGTGA CTGATGGCGG AATTACAAGA GTTTACCCCA AAGAGGCTGG AGAAAACTGG 
CAAGAGAACC CAGAGACGTA TGAGGACAGC TTCTACAAAC GGAGCCTAGA TAACGATAAC 
TACGTTTTCA CTGCGCCCTA CTTCAACAAA AGTGGACCTG GTGCCTATGA ATCTGGAATT 
ATGGTAAGCA AAGCTGTAGA ACTGTACATC CAAGGAAAAC TTCTTAAGCC TGCAGTTGTG 
GGAATTAAAA TTGACGTAAA CTCCTGGATA GAAAATTTTA CCAAAACTTC AATCAGGGAT 
CCGTGTGCTG GTCCAGTTTG TGACTGCAAA AGAAACAGTG ATGTAATGGA CTGTGTCATT 
CTAGATGATG GTGGATTTCT TCTGATGGCC AATCATGATG ATTACACTAA TCAGATTGGA 
CGTTTTTTTG GAGAGATTGA CCCGAGCATG ATGAGACACC TGGTTAATAT ATCACTTTAT 
GCATTCAATA AATCATATGA CTATCAGTCT GTGTGTGATC CAGGGGCAGC ACCAAAGCAA 
GGAGCAGGAC ATCGCTCAGC CTATGTGCCA TCAATTACAG ACATACTCCA GATTGGCTGG 
TGGGCCACTG CTGCCGCCTG GTCTATTCTC CAGCAGCTAC TCTTGAGTTT GACATTTCCA 
CGGCTCCTTG AGGCAGTTGA AATGGAAGAG GATGACTTCA CAGCTTCCCT GTCTAAGCAG 
AGCTGCATCA CAGAACAAAC TCAGTACTTC TTCAAGAACG ATACTAAATC ATTCAGTGGT 
TTACTGGACT GTGGAAACTG TTCCAGGATC TTTCATGTTG AGAAGCTTAT GAACACCAAC 
CTAGTGTTCA TAATGGTGGA GAGCAAGGGA ACATGTCCGT GTGACACGCG GCTGCTCATG 
CAAGCAGAAC AGACTTCTGA TGGTCCAGAT CCTTGCGACA TGGTTAAGCA GCCCAGATAC 
CGAAAAGGAC CTGATGTCTG CTTTGATAAC AATGTGCTGG AGGATTATAC CGACTGTGGT 
GGTGTTTCTG GATTAAACCC TTCCTTATGG TCTATCTTTG GACTCCAATT TATACTCCTT 
TGGCTGGTAT CTGGCAGCAG ACACTATCTA TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d1 ( XM_017592485.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447974.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60772
Clone ID Related Accession (Same CDS sequence)	XM_017592485.1
Accession Version	XM_017592485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTATTGC ATCCAAATCT TCAGCCAAAG CCTATTGGTG TGGGTATACC GACAATTAAT 
TTAAGAAAAA GGAGACCCAA CGTTCAGGAG CCGGTCACAC TGGATTTTCT CGATGCTGAG 
TTAGAGAATG ATATTAAAGT GGAGATTCGA AATAAAATGA TAGACGGAGA AAGTGGAGAA 
AAAACATTCA GAACTCTGGT CAAATCTCAA GATGAGAGAT ATATTGACAA AGGAAATCGA 
ACATACACAT GGACACCTGT CAATGGCACA GATTACAGTT TGGCCTTGGT ATTGCCAACC 
TACAGTTTTT ACTATATAAA AGCCAAAATA GAAGAGACAA TAACTCAGGC CAGATCAAAA 
AAGGGGAAAA TGAAGGATTC AGAAACCCTG AAGCCAGACA ATTTTGAAGA ATCTGGCTAC 
ACTTTCATAG CACCAAGGGA ATACTGCAAT GACCTGAAAC CTTCAGATAA TAACACTGAA 
TTTCTTTTAA ATTTCAATGA ATTTATTGAT AGGAAAACTC CAAACAACCC TTCCTGTAAT 
ACAGATTTGA TTAATAGAAT CTTGCTGGAT GCGGGTTTTA CAAATGAACT TGTCCAAAAT 
TATTGGAGTA AACAGAAAAA TATCAAAGGA GTGAAGGCAC GCTTTGTGGT GACTGATGGT 
GGAATTACAA GAGTTTACCC CAAAGAGGCT GGAGAAAACT GGCAAGAGAA CCCAGAGACG 
TATGAGGACA GCTTCTACAA ACGGAGCCTA GATAACGATA ACTACGTTTT CACTGCGCCC 
TACTTCAACA AAAGTGGACC TGGTGCCTAT GAATCTGGAA TTATGGTAAG CAAAGCTGTA 
GAACTGTACA TCCAAGGAAA ACTTCTTAAG CCTGCAGTTG TGGGAATTAA AATTGACGTA 
AACTCCTGGA TAGAAAATTT TACCAAAACT TCAATCAGGG ATCCGTGTGC TGGTCCAGTT 
TGTGACTGCA AAAGAAACAG TGATGTAATG GACTGTGTCA TTCTAGATGA TGGTGGATTT 
CTTCTGATGG CCAATCATGA TGATTACACT AATCAGATTG GACGTTTTTT TGGAGAGATT 
GACCCGAGCA TGATGAGACA CCTGGTTAAT ATATCACTTT ATGCATTCAA TAAATCATAT 
GACTATCAGT CTGTGTGTGA TCCAGGGGCA GCACCAAAGC AAGGAGCAGG ACATCGCTCA 
GCCTATGTGC CATCGATTAC AGACATACTC CAGATTGGCT GGTGGGCCAC TGCTGCCGCC 
TGGTCTATTC TCCAGCAGCT ACTCTTGAGT TTGACATTTC CACGGCTCCT TGAGGCAGTT 
GAAATGGAAG AGGATGACTT CACAGCTTCC CTGTCTAAGC AGAGCTGTAT CACAGAACAA 
ACTCAGTACT TCTTCAAGAA CGATACTAAA TCATTCAGTG GTTTACTGGA CTGTGGAAAC 
TGTTCCAGGA TCTTTCATGT TGAGAAGCTT ATGAACACCA ACCTAGTGTT CATAATGGTG 
GAGAGCAAGG GAACATGTCC GTGTGACACG CGGCTGCTCA TGCAAGCAGA ACAGACTTCT 
GATGGTCCAG ATCCTTGCGA CATGGTTAAG CAGCCCAGAT ACCGAAAAGG ACCTGATGTC 
TGCTTTGATA ACAATGTGCT GGAGGATTAT ACCGACTGTG GTGGTGTTTC TGGATTAAAC 
CCTTCCTTAT GGTCTATCTT TGGACTCCAA TTTATACTCC TTTGGCTGGT ATCTGGCAGC 
AGACACTATC TATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447974.1
CDS	276..3593
Translation	MAAGCLLALTLTLFQSWLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESESGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTINLRKRRPNVQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKIEETITQARSKKGKMKDSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSITDILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLW

Target ORF information:

RefSeq Version	XM_017592485.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592485.1

ORF Insert Sequence:

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTATTGC ATCCAAATCT TCAGCCAAAG CCTATTGGTG TGGGTATACC GACAATTAAT 
TTAAGAAAAA GGAGACCCAA CGTTCAGGAG CCGGTCACAC TGGATTTTCT CGATGCTGAG 
TTAGAGAATG ATATTAAAGT GGAGATTCGA AATAAAATGA TAGACGGAGA AAGTGGAGAA 
AAAACATTCA GAACTCTGGT CAAATCTCAA GATGAGAGAT ATATTGACAA AGGAAATCGA 
ACATACACAT GGACACCTGT CAATGGCACA GATTACAGTT TGGCCTTGGT ATTGCCAACC 
TACAGTTTTT ACTATATAAA AGCCAAAATA GAAGAGACAA TAACTCAGGC CAGATCAAAA 
AAGGGGAAAA TGAAGGATTC AGAAACCCTG AAGCCAGACA ATTTTGAAGA ATCTGGCTAC 
ACTTTCATAG CACCAAGGGA ATACTGCAAT GACCTGAAAC CTTCAGATAA TAACACTGAA 
TTTCTTTTAA ATTTCAATGA ATTTATTGAT AGGAAAACTC CAAACAACCC TTCCTGTAAT 
ACAGATTTGA TTAATAGAAT CTTGCTGGAT GCGGGTTTTA CAAATGAACT TGTCCAAAAT 
TATTGGAGTA AACAGAAAAA TATCAAAGGA GTGAAGGCAC GCTTTGTGGT GACTGATGGT 
GGAATTACAA GAGTTTACCC CAAAGAGGCT GGAGAAAACT GGCAAGAGAA CCCAGAGACG 
TATGAGGACA GCTTCTACAA ACGGAGCCTA GATAACGATA ACTACGTTTT CACTGCGCCC 
TACTTCAACA AAAGTGGACC TGGTGCCTAT GAATCTGGAA TTATGGTAAG CAAAGCTGTA 
GAACTGTACA TCCAAGGAAA ACTTCTTAAG CCTGCAGTTG TGGGAATTAA AATTGACGTA 
AACTCCTGGA TAGAAAATTT TACCAAAACT TCAATCAGGG ATCCGTGTGC TGGTCCAGTT 
TGTGACTGCA AAAGAAACAG TGATGTAATG GACTGTGTCA TTCTAGATGA TGGTGGATTT 
CTTCTGATGG CCAATCATGA TGATTACACT AATCAGATTG GACGTTTTTT TGGAGAGATT 
GACCCGAGCA TGATGAGACA CCTGGTTAAT ATATCACTTT ATGCATTCAA TAAATCATAT 
GACTATCAGT CTGTGTGTGA TCCAGGGGCA GCACCAAAGC AAGGAGCAGG ACATCGCTCA 
GCCTATGTGC CATCGATTAC AGACATACTC CAGATTGGCT GGTGGGCCAC TGCTGCCGCC 
TGGTCTATTC TCCAGCAGCT ACTCTTGAGT TTGACATTTC CACGGCTCCT TGAGGCAGTT 
GAAATGGAAG AGGATGACTT CACAGCTTCC CTGTCTAAGC AGAGCTGTAT CACAGAACAA 
ACTCAGTACT TCTTCAAGAA CGATACTAAA TCATTCAGTG GTTTACTGGA CTGTGGAAAC 
TGTTCCAGGA TCTTTCATGT TGAGAAGCTT ATGAACACCA ACCTAGTGTT CATAATGGTG 
GAGAGCAAGG GAACATGTCC GTGTGACACG CGGCTGCTCA TGCAAGCAGA ACAGACTTCT 
GATGGTCCAG ATCCTTGCGA CATGGTTAAG CAGCCCAGAT ACCGAAAAGG ACCTGATGTC 
TGCTTTGATA ACAATGTGCT GGAGGATTAT ACCGACTGTG GTGGTGTTTC TGGATTAAAC 
CCTTCCTTAT GGTCTATCTT TGGACTCCAA TTTATACTCC TTTGGCTGGT ATCTGGCAGC 
AGACACTATC TATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d1 ( NM_001110847.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001104317.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 3, mRNA.

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CloneID	ORa00863
Clone ID Related Accession (Same CDS sequence)	NM_001110847.1
Accession Version	NM_001110847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF400662.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF400662.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTACTGC ATCCAAATCT TCAGCCAAAG GAGCCGGTCA CACTGGATTT TCTCGATGCT 
GAGTTAGAGA ATGATATTAA AGTGGAGATT CGAAATAAAA TGATAGACGG AGAAAGTGGA 
GAAAAAACAT TCAGAACTCT GGTCAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAT 
CGAACATACA CATGGACACC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTGCCA 
ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATAT 
TCAGAAACCC TGAAGCCAGA CAATTTTGAA GAATCTGGCT ACACTTTCAT AGCACCAAGG 
GAATACTGCA ATGACCTGAA ACCTTCAGAT AATAACACTG AATTTCTTTT AAATTTCAAT 
GAATTTATTG ATAGGAAAAC TCCAAACAAC CCTTCCTGTA ATACAGATTT GATTAATAGA 
ATCTTGCTGG ATGCGGGTTT TACAAATGAA CTTGTCCAAA ATTATTGGAG TAAACAGAAA 
AATATCAAAG GAGTGAAGGC ACGCTTTGTG GTGACTGATG GCGGAATTAC AAGAGTTTAC 
CCCAAAGAGG CTGGAGAAAA CTGGCAAGAG AACCCAGAGA CGTATGAGGA CAGCTTCTAC 
AAACGGAGCC TAGATAACGA TAACTACGTT TTCACTGCGC CCTACTTCAA CAAAAGTGGA 
CCTGGTGCCT ATGAATCTGG AATTATGGTA AGCAAAGCTG TAGAACTGTA CATCCAAGGA 
AAACTTCTTA AGCCTGCAGT TGTGGGAATT AAAATTGACG TAAACTCCTG GATAGAAAAT 
TTTACCAAAA CTTCAATCAG GGATCCGTGT GCTGGTCCAG TTTGTGACTG CAAAAGAAAC 
AGTGATGTAA TGGACTGTGT CATTCTAGAT GATGGTGGAT TTCTTCTGAT GGCCAATCAT 
GATGATTACA CTAATCAGAT TGGACGTTTT TTTGGAGAGA TTGACCCGAG CATGATGAGA 
CACCTGGTTA ATATATCACT TTATGCATTC AATAAATCAT ATGACTATCA GTCTGTGTGT 
GATCCAGGGG CAGCACCAAA GCAAGGAGCA GGACATCGCT CAGCCTATGT GCCATCAATT 
ACAGACATAC TCCAGATTGG CTGGTGGGCC ACTGCTGCCG CCTGGTCAAT TCTCCAGCAG 
CTACTCTTGA GTTTGACATT TCCACGGCTC CTTGAGGCAG TTGAAATGGA AGAGGATGAC 
TTCACAGCTT CCCTGTCTAA GCAGAGCTGC ATCACAGAAC AAACTCAGTA CTTCTTCAAG 
AACGATACTA AATCATTCAG TGGTTTACTG GACTGTGGAA ACTGTTCCAG GATCTTTCAT 
GTTGAGAAGC TTATGAACAC CAACCTAGTG TTCATAATGG TGGAGAGCAA GGGAACATGT 
CCGTGTGACA CGCGGCTGCT CATGCAAGCA GAACAGACTT CTGATGGTCC AGATCCTTGC 
GACATGGTTA AGCAGCCCAG ATACCGAAAA GGACCTGATG TCTGCTTTGA TAACAATGTG 
CTGGAGGATT ATACCGACTG TGGTGGTGTT TCTGGATTAA ACCCTTCCTT ATGGTCTATC 
TTTGGACTCC AATTTATACT CCTTTGGCTG GTATCTGGCA GCAGACACTA TCTATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104317.1
CDS	13..3252
Translation	MAAGCLLALTLTLFQSWLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESESGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKIEETITQARYSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSITDILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLW

Target ORF information:

RefSeq Version	NM_001110847.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110847.1

ORF Insert Sequence:

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTACTGC ATCCAAATCT TCAGCCAAAG GAGCCGGTCA CACTGGATTT TCTCGATGCT 
GAGTTAGAGA ATGATATTAA AGTGGAGATT CGAAATAAAA TGATAGACGG AGAAAGTGGA 
GAAAAAACAT TCAGAACTCT GGTCAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAT 
CGAACATACA CATGGACACC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTGCCA 
ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATAT 
TCAGAAACCC TGAAGCCAGA CAATTTTGAA GAATCTGGCT ACACTTTCAT AGCACCAAGG 
GAATACTGCA ATGACCTGAA ACCTTCAGAT AATAACACTG AATTTCTTTT AAATTTCAAT 
GAATTTATTG ATAGGAAAAC TCCAAACAAC CCTTCCTGTA ATACAGATTT GATTAATAGA 
ATCTTGCTGG ATGCGGGTTT TACAAATGAA CTTGTCCAAA ATTATTGGAG TAAACAGAAA 
AATATCAAAG GAGTGAAGGC ACGCTTTGTG GTGACTGATG GCGGAATTAC AAGAGTTTAC 
CCCAAAGAGG CTGGAGAAAA CTGGCAAGAG AACCCAGAGA CGTATGAGGA CAGCTTCTAC 
AAACGGAGCC TAGATAACGA TAACTACGTT TTCACTGCGC CCTACTTCAA CAAAAGTGGA 
CCTGGTGCCT ATGAATCTGG AATTATGGTA AGCAAAGCTG TAGAACTGTA CATCCAAGGA 
AAACTTCTTA AGCCTGCAGT TGTGGGAATT AAAATTGACG TAAACTCCTG GATAGAAAAT 
TTTACCAAAA CTTCAATCAG GGATCCGTGT GCTGGTCCAG TTTGTGACTG CAAAAGAAAC 
AGTGATGTAA TGGACTGTGT CATTCTAGAT GATGGTGGAT TTCTTCTGAT GGCCAATCAT 
GATGATTACA CTAATCAGAT TGGACGTTTT TTTGGAGAGA TTGACCCGAG CATGATGAGA 
CACCTGGTTA ATATATCACT TTATGCATTC AATAAATCAT ATGACTATCA GTCTGTGTGT 
GATCCAGGGG CAGCACCAAA GCAAGGAGCA GGACATCGCT CAGCCTATGT GCCATCAATT 
ACAGACATAC TCCAGATTGG CTGGTGGGCC ACTGCTGCCG CCTGGTCAAT TCTCCAGCAG 
CTACTCTTGA GTTTGACATT TCCACGGCTC CTTGAGGCAG TTGAAATGGA AGAGGATGAC 
TTCACAGCTT CCCTGTCTAA GCAGAGCTGC ATCACAGAAC AAACTCAGTA CTTCTTCAAG 
AACGATACTA AATCATTCAG TGGTTTACTG GACTGTGGAA ACTGTTCCAG GATCTTTCAT 
GTTGAGAAGC TTATGAACAC CAACCTAGTG TTCATAATGG TGGAGAGCAA GGGAACATGT 
CCGTGTGACA CGCGGCTGCT CATGCAAGCA GAACAGACTT CTGATGGTCC AGATCCTTGC 
GACATGGTTA AGCAGCCCAG ATACCGAAAA GGACCTGATG TCTGCTTTGA TAACAATGTG 
CTGGAGGATT ATACCGACTG TGGTGGTGTT TCTGGATTAA ACCCTTCCTT ATGGTCTATC 
TTTGGACTCC AATTTATACT CCTTTGGCTG GTATCTGGCA GCAGACACTA TCTATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d1 ( NM_001110848.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001104318.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 2, mRNA.

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CloneID	ORa00867
Clone ID Related Accession (Same CDS sequence)	NM_001110848.1
Accession Version	NM_001110848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF486276.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF486276.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTACTGC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC GGTCACACTG 
GATTTTCTCG ATGCTGAGTT AGAGAATGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA 
GACGGAGAAA GTGGAGAAAA AACATTCAGA ACTCTGGTCA AATCTCAAGA TGAGAGATTT 
ATTGACAAAG GAAATCGAAC ATACACATGG ACACCTGTCA ATGGCACAGA TTACAGTTTG 
GCCTTGGTAT TGCCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA 
ACTCAGGCCA GATATTCAGA AACCCTGAAG CCAGACAATT TTGAAGAATC TGGCTACACT 
TTCATAGCAC CAAGGGAATA CTGCAATGAC CTGAAACCTT CAGATAATAA CACTGAATTT 
CTTTTAAATT TCAATGAATT TATTGATAGG AAAACTCCAA ACAACCCTTC CTGTAATACA 
GATTTGATTA ATAGAATCTT GCTGGATGCG GGTTTTACAA ATGAACTTGT CCAAAATTAT 
TGGAGTAAAC AGAAAAATAT CAAAGGAGTG AAGGCACGCT TTGTGGTGAC TGATGGCGGA 
ATTACAAGAG TTTACCCCAA AGAGGCTGGA GAAAACTGGC AAGAGAACCC AGAGACGTAT 
GAGGACAGCT TCTACAAACG GAGCCTAGAT AACGATAACT ACGTTTTCAC TGCGCCCTAC 
TTCAACAAAA GTGGACCTGG TGCCTATGAA TCTGGAATTA TGGTAAGCAA AGCTGTAGAA 
CTGTACATCC AAGGAAAACT TCTTAAGCCT GCAGTTGTGG GAATTAAAAT TGACGTAAAC 
TCCTGGATAG AAAATTTTAC CAAAACTTCA ATCAGGGATC CGTGTGCTGG TCCAGTTTGT 
GACTGCAAAA GAAACAGTGA TGTAATGGAC TGTGTCATTC TAGATGATGG TGGATTTCTT 
CTGATGGCCA ATCATGATGA TTACACTAAT CAGATTGGAC GTTTTTTTGG AGAGATTGAC 
CCGAGCATGA TGAGACACCT GGTTAATATA TCACTTTATG CATTCAATAA ATCATATGAC 
TATCAGTCTG TGTGTGATCC AGGGGCAGCA CCAAAGCAAG GAGCAGGACA TCGCTCAGCC 
TATGTGCCAT CAATTACAGA CATACTCCAG ATTGGCTGGT GGGCCACTGC TGCCGCCTGG 
TCAATTCTCC AGCAGCTACT CTTGAGTTTG ACATTTCCAC GGCTCCTTGA GGCAGTTGAA 
ATGGAAGAGG ATGACTTCAC AGCTTCCCTG TCTAAGCAGA GCTGCATCAC AGAACAAACT 
CAGTACTTCT TCAAGAACGA TACTAAATCA TTCAGTGGTT TACTGGACTG TGGAAACTGT 
TCCAGGATCT TTCATGTTGA GAAGCTTATG AACACCAACC TAGTGTTCAT AATGGTGGAG 
AGCAAGGGAA CATGTCCGTG TGACACGCGG CTGCTCATGC AAGCAGAACA GACTTCTGAT 
GGTCCAGATC CTTGCGACAT GGTTAAGCAG CCCAGATACC GAAAAGGACC TGATGTCTGC 
TTTGATAACA ATGTGCTGGA GGATTATACC GACTGTGGTG GTGTTTCTGG ATTAAACCCT 
TCCTTATGGT CTATCTTTGG ACTCCAATTT ATACTCCTTT GGCTGGTATC TGGCAGCAGA 
CACTATCTAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104318.1
CDS	1..3255
Translation	MAAGCLLALTLTLFQSWLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESESGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERFIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKIEETITQARYSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSITDILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLW

Target ORF information:

RefSeq Version	NM_001110848.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110848.1

ORF Insert Sequence:

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTACTGC ATCCAAATCT TCAGCCAAAG AACCCCAAAT CTCAGGAGCC GGTCACACTG 
GATTTTCTCG ATGCTGAGTT AGAGAATGAT ATTAAAGTGG AGATTCGAAA TAAAATGATA 
GACGGAGAAA GTGGAGAAAA AACATTCAGA ACTCTGGTCA AATCTCAAGA TGAGAGATTT 
ATTGACAAAG GAAATCGAAC ATACACATGG ACACCTGTCA ATGGCACAGA TTACAGTTTG 
GCCTTGGTAT TGCCAACCTA CAGTTTTTAC TATATAAAAG CCAAAATAGA AGAGACAATA 
ACTCAGGCCA GATATTCAGA AACCCTGAAG CCAGACAATT TTGAAGAATC TGGCTACACT 
TTCATAGCAC CAAGGGAATA CTGCAATGAC CTGAAACCTT CAGATAATAA CACTGAATTT 
CTTTTAAATT TCAATGAATT TATTGATAGG AAAACTCCAA ACAACCCTTC CTGTAATACA 
GATTTGATTA ATAGAATCTT GCTGGATGCG GGTTTTACAA ATGAACTTGT CCAAAATTAT 
TGGAGTAAAC AGAAAAATAT CAAAGGAGTG AAGGCACGCT TTGTGGTGAC TGATGGCGGA 
ATTACAAGAG TTTACCCCAA AGAGGCTGGA GAAAACTGGC AAGAGAACCC AGAGACGTAT 
GAGGACAGCT TCTACAAACG GAGCCTAGAT AACGATAACT ACGTTTTCAC TGCGCCCTAC 
TTCAACAAAA GTGGACCTGG TGCCTATGAA TCTGGAATTA TGGTAAGCAA AGCTGTAGAA 
CTGTACATCC AAGGAAAACT TCTTAAGCCT GCAGTTGTGG GAATTAAAAT TGACGTAAAC 
TCCTGGATAG AAAATTTTAC CAAAACTTCA ATCAGGGATC CGTGTGCTGG TCCAGTTTGT 
GACTGCAAAA GAAACAGTGA TGTAATGGAC TGTGTCATTC TAGATGATGG TGGATTTCTT 
CTGATGGCCA ATCATGATGA TTACACTAAT CAGATTGGAC GTTTTTTTGG AGAGATTGAC 
CCGAGCATGA TGAGACACCT GGTTAATATA TCACTTTATG CATTCAATAA ATCATATGAC 
TATCAGTCTG TGTGTGATCC AGGGGCAGCA CCAAAGCAAG GAGCAGGACA TCGCTCAGCC 
TATGTGCCAT CAATTACAGA CATACTCCAG ATTGGCTGGT GGGCCACTGC TGCCGCCTGG 
TCAATTCTCC AGCAGCTACT CTTGAGTTTG ACATTTCCAC GGCTCCTTGA GGCAGTTGAA 
ATGGAAGAGG ATGACTTCAC AGCTTCCCTG TCTAAGCAGA GCTGCATCAC AGAACAAACT 
CAGTACTTCT TCAAGAACGA TACTAAATCA TTCAGTGGTT TACTGGACTG TGGAAACTGT 
TCCAGGATCT TTCATGTTGA GAAGCTTATG AACACCAACC TAGTGTTCAT AATGGTGGAG 
AGCAAGGGAA CATGTCCGTG TGACACGCGG CTGCTCATGC AAGCAGAACA GACTTCTGAT 
GGTCCAGATC CTTGCGACAT GGTTAAGCAG CCCAGATACC GAAAAGGACC TGATGTCTGC 
TTTGATAACA ATGTGCTGGA GGATTATACC GACTGTGGTG GTGTTTCTGG ATTAAACCCT 
TCCTTATGGT CTATCTTTGG ACTCCAATTT ATACTCCTTT GGCTGGTATC TGGCAGCAGA 
CACTATCTAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d1 ( XM_006235981.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236043.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X1, mRNA.

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CloneID	ORa26958
Clone ID Related Accession (Same CDS sequence)	XM_006235981.2
Accession Version	XM_006235981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006235981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTATTGC ATCCAAATCT TCAGCCAAAG CCTATTGGTG TGGGTATACC GACAATTAAT 
TTAAGAAAAA GGAGACCCAA CGTTCAGAAC CCCAAATCTC AGGAGCCGGT CACACTGGAT 
TTTCTCGATG CTGAGTTAGA GAATGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAC 
GGAGAAAGTG GAGAAAAAAC ATTCAGAACT CTGGTCAAAT CTCAAGATGA GAGATATATT 
GACAAAGGAA ATCGAACATA CACATGGACA CCTGTCAATG GCACAGATTA CAGTTTGGCC 
TTGGTATTGC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT 
CAGGCCAGAT CAAAAAAGGG GAAAATGAAG GATTCAGAAA CCCTGAAGCC AGACAATTTT 
GAAGAATCTG GCTACACTTT CATAGCACCA AGGGAATACT GCAATGACCT GAAACCTTCA 
GATAATAACA CTGAATTTCT TTTAAATTTC AATGAATTTA TTGATAGGAA AACTCCAAAC 
AACCCTTCCT GTAATACAGA TTTGATTAAT AGAATCTTGC TGGATGCGGG TTTTACAAAT 
GAACTTGTCC AAAATTATTG GAGTAAACAG AAAAATATCA AAGGAGTGAA GGCACGCTTT 
GTGGTGACTG ATGGTGGAAT TACAAGAGTT TACCCCAAAG AGGCTGGAGA AAACTGGCAA 
GAGAACCCAG AGACGTATGA GGACAGCTTC TACAAACGGA GCCTAGATAA CGATAACTAC 
GTTTTCACTG CGCCCTACTT CAACAAAAGT GGACCTGGTG CCTATGAATC TGGAATTATG 
GTAAGCAAAG CTGTAGAACT GTACATCCAA GGAAAACTTC TTAAGCCTGC AGTTGTGGGA 
ATTAAAATTG ACGTAAACTC CTGGATAGAA AATTTTACCA AAACTTCAAT CAGGGATCCG 
TGTGCTGGTC CAGTTTGTGA CTGCAAAAGA AACAGTGATG TAATGGACTG TGTCATTCTA 
GATGATGGTG GATTTCTTCT GATGGCCAAT CATGATGATT ACACTAATCA GATTGGACGT 
TTTTTTGGAG AGATTGACCC GAGCATGATG AGACACCTGG TTAATATATC ACTTTATGCA 
TTCAATAAAT CATATGACTA TCAGTCTGTG TGTGATCCAG GGGCAGCACC AAAGCAAGGA 
GCAGGACATC GCTCAGCCTA TGTGCCATCG ATTACAGACA TACTCCAGAT TGGCTGGTGG 
GCCACTGCTG CCGCCTGGTC TATTCTCCAG CAGCTACTCT TGAGTTTGAC ATTTCCACGG 
CTCCTTGAGG CAGTTGAAAT GGAAGAGGAT GACTTCACAG CTTCCCTGTC TAAGCAGAGC 
TGTATCACAG AACAAACTCA GTACTTCTTC AAGAACGATA CTAAATCATT CAGTGGTTTA 
CTGGACTGTG GAAACTGTTC CAGGATCTTT CATGTTGAGA AGCTTATGAA CACCAACCTA 
GTGTTCATAA TGGTGGAGAG CAAGGGAACA TGTCCGTGTG ACACGCGGCT GCTCATGCAA 
GCAGAACAGA CTTCTGATGG TCCAGATCCT TGCGACATGG TTAAGCAGCC CAGATACCGA 
AAAGGACCTG ATGTCTGCTT TGATAACAAT GTGCTGGAGG ATTATACCGA CTGTGGTGGT 
GTTTCTGGAT TAAACCCTTC CTTATGGTCT ATCTTTGGAC TCCAATTTAT ACTCCTTTGG 
CTGGTATCTG GCAGCAGACA CTATCTATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236043.1
CDS	276..3608
Translation	MAAGCLLALTLTLFQSWLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESESGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTINLRKRRPNVQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKIEETITQARSKKGKMKDSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSITDILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLW

Target ORF information:

RefSeq Version	XM_006235981.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235981.2

ORF Insert Sequence:

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTATTGC ATCCAAATCT TCAGCCAAAG CCTATTGGTG TGGGTATACC GACAATTAAT 
TTAAGAAAAA GGAGACCCAA CGTTCAGAAC CCCAAATCTC AGGAGCCGGT CACACTGGAT 
TTTCTCGATG CTGAGTTAGA GAATGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAC 
GGAGAAAGTG GAGAAAAAAC ATTCAGAACT CTGGTCAAAT CTCAAGATGA GAGATATATT 
GACAAAGGAA ATCGAACATA CACATGGACA CCTGTCAATG GCACAGATTA CAGTTTGGCC 
TTGGTATTGC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT 
CAGGCCAGAT CAAAAAAGGG GAAAATGAAG GATTCAGAAA CCCTGAAGCC AGACAATTTT 
GAAGAATCTG GCTACACTTT CATAGCACCA AGGGAATACT GCAATGACCT GAAACCTTCA 
GATAATAACA CTGAATTTCT TTTAAATTTC AATGAATTTA TTGATAGGAA AACTCCAAAC 
AACCCTTCCT GTAATACAGA TTTGATTAAT AGAATCTTGC TGGATGCGGG TTTTACAAAT 
GAACTTGTCC AAAATTATTG GAGTAAACAG AAAAATATCA AAGGAGTGAA GGCACGCTTT 
GTGGTGACTG ATGGTGGAAT TACAAGAGTT TACCCCAAAG AGGCTGGAGA AAACTGGCAA 
GAGAACCCAG AGACGTATGA GGACAGCTTC TACAAACGGA GCCTAGATAA CGATAACTAC 
GTTTTCACTG CGCCCTACTT CAACAAAAGT GGACCTGGTG CCTATGAATC TGGAATTATG 
GTAAGCAAAG CTGTAGAACT GTACATCCAA GGAAAACTTC TTAAGCCTGC AGTTGTGGGA 
ATTAAAATTG ACGTAAACTC CTGGATAGAA AATTTTACCA AAACTTCAAT CAGGGATCCG 
TGTGCTGGTC CAGTTTGTGA CTGCAAAAGA AACAGTGATG TAATGGACTG TGTCATTCTA 
GATGATGGTG GATTTCTTCT GATGGCCAAT CATGATGATT ACACTAATCA GATTGGACGT 
TTTTTTGGAG AGATTGACCC GAGCATGATG AGACACCTGG TTAATATATC ACTTTATGCA 
TTCAATAAAT CATATGACTA TCAGTCTGTG TGTGATCCAG GGGCAGCACC AAAGCAAGGA 
GCAGGACATC GCTCAGCCTA TGTGCCATCG ATTACAGACA TACTCCAGAT TGGCTGGTGG 
GCCACTGCTG CCGCCTGGTC TATTCTCCAG CAGCTACTCT TGAGTTTGAC ATTTCCACGG 
CTCCTTGAGG CAGTTGAAAT GGAAGAGGAT GACTTCACAG CTTCCCTGTC TAAGCAGAGC 
TGTATCACAG AACAAACTCA GTACTTCTTC AAGAACGATA CTAAATCATT CAGTGGTTTA 
CTGGACTGTG GAAACTGTTC CAGGATCTTT CATGTTGAGA AGCTTATGAA CACCAACCTA 
GTGTTCATAA TGGTGGAGAG CAAGGGAACA TGTCCGTGTG ACACGCGGCT GCTCATGCAA 
GCAGAACAGA CTTCTGATGG TCCAGATCCT TGCGACATGG TTAAGCAGCC CAGATACCGA 
AAAGGACCTG ATGTCTGCTT TGATAACAAT GTGCTGGAGG ATTATACCGA CTGTGGTGGT 
GTTTCTGGAT TAAACCCTTC CTTATGGTCT ATCTTTGGAC TCCAATTTAT ACTCCTTTGG 
CTGGTATCTG GCAGCAGACA CTATCTATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d1 ( XM_006235982.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236044.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa26959
Clone ID Related Accession (Same CDS sequence)	XM_006235982.3
Accession Version	XM_006235982.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235982.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTATTGC ATCCAAATCT TCAGCCAAAG CCTATTGGTG TGGGTATACC GACAATTAAT 
TTAAGAAAAA GGAGACCCAA CGTTCAGAAC CCCAAATCTC AGGAGCCGGT CACACTGGAT 
TTTCTCGATG CTGAGTTAGA GAATGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAC 
GGAGAAAGTG GAGAAAAAAC ATTCAGAACT CTGGTCAAAT CTCAAGATGA GAGATATATT 
GACAAAGGAA ATCGAACATA CACATGGACA CCTGTCAATG GCACAGATTA CAGTTTGGCC 
TTGGTATTGC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT 
CAGGCCAGAT ATTCAGAAAC CCTGAAGCCA GACAATTTTG AAGAATCTGG CTACACTTTC 
ATAGCACCAA GGGAATACTG CAATGACCTG AAACCTTCAG ATAATAACAC TGAATTTCTT 
TTAAATTTCA ATGAATTTAT TGATAGGAAA ACTCCAAACA ACCCTTCCTG TAATACAGAT 
TTGATTAATA GAATCTTGCT GGATGCGGGT TTTACAAATG AACTTGTCCA AAATTATTGG 
AGTAAACAGA AAAATATCAA AGGAGTGAAG GCACGCTTTG TGGTGACTGA TGGTGGAATT 
ACAAGAGTTT ACCCCAAAGA GGCTGGAGAA AACTGGCAAG AGAACCCAGA GACGTATGAG 
GACAGCTTCT ACAAACGGAG CCTAGATAAC GATAACTACG TTTTCACTGC GCCCTACTTC 
AACAAAAGTG GACCTGGTGC CTATGAATCT GGAATTATGG TAAGCAAAGC TGTAGAACTG 
TACATCCAAG GAAAACTTCT TAAGCCTGCA GTTGTGGGAA TTAAAATTGA CGTAAACTCC 
TGGATAGAAA ATTTTACCAA AACTTCAATC AGGGATCCGT GTGCTGGTCC AGTTTGTGAC 
TGCAAAAGAA ACAGTGATGT AATGGACTGT GTCATTCTAG ATGATGGTGG ATTTCTTCTG 
ATGGCCAATC ATGATGATTA CACTAATCAG ATTGGACGTT TTTTTGGAGA GATTGACCCG 
AGCATGATGA GACACCTGGT TAATATATCA CTTTATGCAT TCAATAAATC ATATGACTAT 
CAGTCTGTGT GTGATCCAGG GGCAGCACCA AAGCAAGGAG CAGGACATCG CTCAGCCTAT 
GTGCCATCGA TTACAGACAT ACTCCAGATT GGCTGGTGGG CCACTGCTGC CGCCTGGTCT 
ATTCTCCAGC AGCTACTCTT GAGTTTGACA TTTCCACGGC TCCTTGAGGC AGTTGAAATG 
GAAGAGGATG ACTTCACAGC TTCCCTGTCT AAGCAGAGCT GTATCACAGA ACAAACTCAG 
TACTTCTTCA AGAACGATAC TAAATCATTC AGTGGTTTAC TGGACTGTGG AAACTGTTCC 
AGGATCTTTC ATGTTGAGAA GCTTATGAAC ACCAACCTAG TGTTCATAAT GGTGGAGAGC 
AAGGGAACAT GTCCGTGTGA CACGCGGCTG CTCATGCAAG CAGAACAGAC TTCTGATGGT 
CCAGATCCTT GCGACATGGT TAAGCAGCCC AGATACCGAA AAGGACCTGA TGTCTGCTTT 
GATAACAATG TGCTGGAGGA TTATACCGAC TGTGGTGGTG TTTCTGGATT AAACCCTTCC 
TTATGGTCTA TCTTTGGACT CCAATTTATA CTCCTTTGGC TGGTATCTGG CAGCAGACAC 
TATCTATGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236044.1
CDS	276..3587
Translation	MAAGCLLALTLTLFQSWLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESESGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTINLRKRRPNVQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKIEETITQARYSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSITDILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLW

Target ORF information:

RefSeq Version	XM_006235982.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235982.3

ORF Insert Sequence:

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTATTGC ATCCAAATCT TCAGCCAAAG CCTATTGGTG TGGGTATACC GACAATTAAT 
TTAAGAAAAA GGAGACCCAA CGTTCAGAAC CCCAAATCTC AGGAGCCGGT CACACTGGAT 
TTTCTCGATG CTGAGTTAGA GAATGATATT AAAGTGGAGA TTCGAAATAA AATGATAGAC 
GGAGAAAGTG GAGAAAAAAC ATTCAGAACT CTGGTCAAAT CTCAAGATGA GAGATATATT 
GACAAAGGAA ATCGAACATA CACATGGACA CCTGTCAATG GCACAGATTA CAGTTTGGCC 
TTGGTATTGC CAACCTACAG TTTTTACTAT ATAAAAGCCA AAATAGAAGA GACAATAACT 
CAGGCCAGAT ATTCAGAAAC CCTGAAGCCA GACAATTTTG AAGAATCTGG CTACACTTTC 
ATAGCACCAA GGGAATACTG CAATGACCTG AAACCTTCAG ATAATAACAC TGAATTTCTT 
TTAAATTTCA ATGAATTTAT TGATAGGAAA ACTCCAAACA ACCCTTCCTG TAATACAGAT 
TTGATTAATA GAATCTTGCT GGATGCGGGT TTTACAAATG AACTTGTCCA AAATTATTGG 
AGTAAACAGA AAAATATCAA AGGAGTGAAG GCACGCTTTG TGGTGACTGA TGGTGGAATT 
ACAAGAGTTT ACCCCAAAGA GGCTGGAGAA AACTGGCAAG AGAACCCAGA GACGTATGAG 
GACAGCTTCT ACAAACGGAG CCTAGATAAC GATAACTACG TTTTCACTGC GCCCTACTTC 
AACAAAAGTG GACCTGGTGC CTATGAATCT GGAATTATGG TAAGCAAAGC TGTAGAACTG 
TACATCCAAG GAAAACTTCT TAAGCCTGCA GTTGTGGGAA TTAAAATTGA CGTAAACTCC 
TGGATAGAAA ATTTTACCAA AACTTCAATC AGGGATCCGT GTGCTGGTCC AGTTTGTGAC 
TGCAAAAGAA ACAGTGATGT AATGGACTGT GTCATTCTAG ATGATGGTGG ATTTCTTCTG 
ATGGCCAATC ATGATGATTA CACTAATCAG ATTGGACGTT TTTTTGGAGA GATTGACCCG 
AGCATGATGA GACACCTGGT TAATATATCA CTTTATGCAT TCAATAAATC ATATGACTAT 
CAGTCTGTGT GTGATCCAGG GGCAGCACCA AAGCAAGGAG CAGGACATCG CTCAGCCTAT 
GTGCCATCGA TTACAGACAT ACTCCAGATT GGCTGGTGGG CCACTGCTGC CGCCTGGTCT 
ATTCTCCAGC AGCTACTCTT GAGTTTGACA TTTCCACGGC TCCTTGAGGC AGTTGAAATG 
GAAGAGGATG ACTTCACAGC TTCCCTGTCT AAGCAGAGCT GTATCACAGA ACAAACTCAG 
TACTTCTTCA AGAACGATAC TAAATCATTC AGTGGTTTAC TGGACTGTGG AAACTGTTCC 
AGGATCTTTC ATGTTGAGAA GCTTATGAAC ACCAACCTAG TGTTCATAAT GGTGGAGAGC 
AAGGGAACAT GTCCGTGTGA CACGCGGCTG CTCATGCAAG CAGAACAGAC TTCTGATGGT 
CCAGATCCTT GCGACATGGT TAAGCAGCCC AGATACCGAA AAGGACCTGA TGTCTGCTTT 
GATAACAATG TGCTGGAGGA TTATACCGAC TGTGGTGGTG TTTCTGGATT AAACCCTTCC 
TTATGGTCTA TCTTTGGACT CCAATTTATA CTCCTTTGGC TGGTATCTGG CAGCAGACAC 
TATCTATGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d1 ( XM_006235983.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236045.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa26960
Clone ID Related Accession (Same CDS sequence)	XM_006235983.3
Accession Version	XM_006235983.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTATTGC ATCCAAATCT TCAGCCAAAG GAGCCGGTCA CACTGGATTT TCTCGATGCT 
GAGTTAGAGA ATGATATTAA AGTGGAGATT CGAAATAAAA TGATAGACGG AGAAAGTGGA 
GAAAAAACAT TCAGAACTCT GGTCAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAT 
CGAACATACA CATGGACACC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTGCCA 
ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATCA 
AAAAAGGGGA AAATGAAGGA TTCAGAAACC CTGAAGCCAG ACAATTTTGA AGAATCTGGC 
TACACTTTCA TAGCACCAAG GGAATACTGC AATGACCTGA AACCTTCAGA TAATAACACT 
GAATTTCTTT TAAATTTCAA TGAATTTATT GATAGGAAAA CTCCAAACAA CCCTTCCTGT 
AATACAGATT TGATTAATAG AATCTTGCTG GATGCGGGTT TTACAAATGA ACTTGTCCAA 
AATTATTGGA GTAAACAGAA AAATATCAAA GGAGTGAAGG CACGCTTTGT GGTGACTGAT 
GGTGGAATTA CAAGAGTTTA CCCCAAAGAG GCTGGAGAAA ACTGGCAAGA GAACCCAGAG 
ACGTATGAGG ACAGCTTCTA CAAACGGAGC CTAGATAACG ATAACTACGT TTTCACTGCG 
CCCTACTTCA ACAAAAGTGG ACCTGGTGCC TATGAATCTG GAATTATGGT AAGCAAAGCT 
GTAGAACTGT ACATCCAAGG AAAACTTCTT AAGCCTGCAG TTGTGGGAAT TAAAATTGAC 
GTAAACTCCT GGATAGAAAA TTTTACCAAA ACTTCAATCA GGGATCCGTG TGCTGGTCCA 
GTTTGTGACT GCAAAAGAAA CAGTGATGTA ATGGACTGTG TCATTCTAGA TGATGGTGGA 
TTTCTTCTGA TGGCCAATCA TGATGATTAC ACTAATCAGA TTGGACGTTT TTTTGGAGAG 
ATTGACCCGA GCATGATGAG ACACCTGGTT AATATATCAC TTTATGCATT CAATAAATCA 
TATGACTATC AGTCTGTGTG TGATCCAGGG GCAGCACCAA AGCAAGGAGC AGGACATCGC 
TCAGCCTATG TGCCATCGAT TACAGACATA CTCCAGATTG GCTGGTGGGC CACTGCTGCC 
GCCTGGTCTA TTCTCCAGCA GCTACTCTTG AGTTTGACAT TTCCACGGCT CCTTGAGGCA 
GTTGAAATGG AAGAGGATGA CTTCACAGCT TCCCTGTCTA AGCAGAGCTG TATCACAGAA 
CAAACTCAGT ACTTCTTCAA GAACGATACT AAATCATTCA GTGGTTTACT GGACTGTGGA 
AACTGTTCCA GGATCTTTCA TGTTGAGAAG CTTATGAACA CCAACCTAGT GTTCATAATG 
GTGGAGAGCA AGGGAACATG TCCGTGTGAC ACGCGGCTGC TCATGCAAGC AGAACAGACT 
TCTGATGGTC CAGATCCTTG CGACATGGTT AAGCAGCCCA GATACCGAAA AGGACCTGAT 
GTCTGCTTTG ATAACAATGT GCTGGAGGAT TATACCGACT GTGGTGGTGT TTCTGGATTA 
AACCCTTCCT TATGGTCTAT CTTTGGACTC CAATTTATAC TCCTTTGGCT GGTATCTGGC 
AGCAGACACT ATCTATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236045.1
CDS	276..3536
Translation	MAAGCLLALTLTLFQSWLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESESGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKIEETITQARSKKGKMKDSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSITDILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLW

Target ORF information:

RefSeq Version	XM_006235983.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 1 (Cacna2d1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235983.3

ORF Insert Sequence:

ATGGCTGCTG GCTGCCTGCT GGCCTTGACT CTGACACTTT TCCAATCTTG GCTGATCGGC 
CCCTCGAGCG AGGAGCCCTT CCCTTCGCCC GTCACTATCA AGTCATGGGT GGATAAAATG 
CAGGAAGACC TTGTCACACT GGCAAAGACA GCAAGTGGAG TGACTCAGCT TGCTGACATT 
TATGAAAAAT ACCAAGATTT GTATACTGTG GAGCCAAATA ATGCACGCCA ACTGGTTGAA 
ATTGCAGCTA GAGACATTGA GAAACTTCTG AGCAACAGAT CTAAAGCCCT GGTGCGCCTG 
GCTATGGAAG CAGAGAAAGT CCAAGCCGCC CACCAATGGA GGGAAGATTT TGCAAGCAAC 
GAAGTTGTCT ACTACAACGC TAAAGACGAT CTTGATCCTG AAAGAAATGA GAGTGAGTCA 
GGCAGCCAAC GGATTAAACC TGTTTTCATC GAAGACGCTA ATTTTGGACG CCAGATATCC 
TATCAACATG CAGCGGTCCA TATTCCCACG GACATCTATG AGGGCTCAAC CATAGTGTTA 
AACGAACTCA ACTGGACAAG TGCCTTAGAT GAAGTTTTCA AAAGAAATCG AGATGAAGAC 
CCTACATTGC TGTGGCAAGT GTTTGGCAGC GCCACCGGCC TGGCCCGATA TTATCCAGCT 
TCTCCGTGGG TGGATAACAG CAGAACTCCA AACAAGATCG ATCTCTACGA TGTACGCAGA 
AGACCATGGT ACATCCAGGG AGCTGCATCC CCCAAAGACA TGCTCATTCT GGTGGACGTG 
AGTGGAAGTG TGAGCGGATT GACTCTGAAA CTCATCCGGA CATCTGTCTC TGAGATGTTA 
GAAACCCTTT CCGATGATGA TTTCGTGAAT GTAGCTTCAT TTAACAGCAA TGCTCAGGAT 
GTGAGTTGTT TCCAGCACCT GGTTCAAGCG AATGTAAGGA ACAAGAAGGT GCTGAAGGAT 
GCGGTGAATA ACATCACAGC AAAGGGGATA ACAGATTACA AGAAAGGCTT TAGCTTCGCG 
TTTGAACAGC TACTTAATTA TAATGTTTCC AGAGCCAATT GTAATAAGAT CATCATGTTA 
TTCACGGATG GAGGAGAAGA GAGAGCCCAG GAGATATTTG CCAAGTACAA TAAAGACAAA 
AAAGTCCGCG TGTTTACATT TTCTGTTGGT CAACATAATT ATGACAGAGG ACCTATTCAG 
TGGATGGCTT GTGAAAACAA AGGTTATTAT TATGAGATTC CCTCTATTGG TGCAATAAGA 
ATCAATACTC AGGAATATCT AGATGTTCTG GGAAGACCAA TGGTTTTAGC CGGGGACAAA 
GCAAAGCAAG TCCAGTGGAC AAATGTGTAT TTGGATGCTC TGGAGCTGGG ACTTGTCATT 
ACTGGAACTC TACCGGTCTT CAACGTCACT GGCCAGTCTG AAAATAAGAC TAACTTGAAG 
AACCAGTTGA TTCTTGGTGT GATGGGCGTT GATGTGTCTC TGGAAGATAT TAAGAGATTG 
ACACCACGTT TTACACTGTG CCCCAACGGT TACTATTTTG CAATCGATCC TAATGGTTAT 
GTCTTATTGC ATCCAAATCT TCAGCCAAAG GAGCCGGTCA CACTGGATTT TCTCGATGCT 
GAGTTAGAGA ATGATATTAA AGTGGAGATT CGAAATAAAA TGATAGACGG AGAAAGTGGA 
GAAAAAACAT TCAGAACTCT GGTCAAATCT CAAGATGAGA GATATATTGA CAAAGGAAAT 
CGAACATACA CATGGACACC TGTCAATGGC ACAGATTACA GTTTGGCCTT GGTATTGCCA 
ACCTACAGTT TTTACTATAT AAAAGCCAAA ATAGAAGAGA CAATAACTCA GGCCAGATCA 
AAAAAGGGGA AAATGAAGGA TTCAGAAACC CTGAAGCCAG ACAATTTTGA AGAATCTGGC 
TACACTTTCA TAGCACCAAG GGAATACTGC AATGACCTGA AACCTTCAGA TAATAACACT 
GAATTTCTTT TAAATTTCAA TGAATTTATT GATAGGAAAA CTCCAAACAA CCCTTCCTGT 
AATACAGATT TGATTAATAG AATCTTGCTG GATGCGGGTT TTACAAATGA ACTTGTCCAA 
AATTATTGGA GTAAACAGAA AAATATCAAA GGAGTGAAGG CACGCTTTGT GGTGACTGAT 
GGTGGAATTA CAAGAGTTTA CCCCAAAGAG GCTGGAGAAA ACTGGCAAGA GAACCCAGAG 
ACGTATGAGG ACAGCTTCTA CAAACGGAGC CTAGATAACG ATAACTACGT TTTCACTGCG 
CCCTACTTCA ACAAAAGTGG ACCTGGTGCC TATGAATCTG GAATTATGGT AAGCAAAGCT 
GTAGAACTGT ACATCCAAGG AAAACTTCTT AAGCCTGCAG TTGTGGGAAT TAAAATTGAC 
GTAAACTCCT GGATAGAAAA TTTTACCAAA ACTTCAATCA GGGATCCGTG TGCTGGTCCA 
GTTTGTGACT GCAAAAGAAA CAGTGATGTA ATGGACTGTG TCATTCTAGA TGATGGTGGA 
TTTCTTCTGA TGGCCAATCA TGATGATTAC ACTAATCAGA TTGGACGTTT TTTTGGAGAG 
ATTGACCCGA GCATGATGAG ACACCTGGTT AATATATCAC TTTATGCATT CAATAAATCA 
TATGACTATC AGTCTGTGTG TGATCCAGGG GCAGCACCAA AGCAAGGAGC AGGACATCGC 
TCAGCCTATG TGCCATCGAT TACAGACATA CTCCAGATTG GCTGGTGGGC CACTGCTGCC 
GCCTGGTCTA TTCTCCAGCA GCTACTCTTG AGTTTGACAT TTCCACGGCT CCTTGAGGCA 
GTTGAAATGG AAGAGGATGA CTTCACAGCT TCCCTGTCTA AGCAGAGCTG TATCACAGAA 
CAAACTCAGT ACTTCTTCAA GAACGATACT AAATCATTCA GTGGTTTACT GGACTGTGGA 
AACTGTTCCA GGATCTTTCA TGTTGAGAAG CTTATGAACA CCAACCTAGT GTTCATAATG 
GTGGAGAGCA AGGGAACATG TCCGTGTGAC ACGCGGCTGC TCATGCAAGC AGAACAGACT 
TCTGATGGTC CAGATCCTTG CGACATGGTT AAGCAGCCCA GATACCGAAA AGGACCTGAT 
GTCTGCTTTG ATAACAATGT GCTGGAGGAT TATACCGACT GTGGTGGTGT TTCTGGATTA 
AACCCTTCCT TATGGTCTAT CTTTGGACTC CAATTTATAC TCCTTTGGCT GGTATCTGGC 
AGCAGACACT ATCTATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of calcium channel alpha-2-delta 1 subunit in dorsal horn contributes to spinal cord injury-induced tactile allodynia.
The spine journal : official journal of the North American Spine Society(2018 Jan)
Kusuyama K,Tachibana T,Yamanaka H,Okubo M,Yoshiya S,Noguchi K

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Cacna2d1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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