cacna2d1a cDNA ORF clone, Danio rerio(zebrafish)

The following cacna2d1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna2d1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa00690 NM_001044960.2
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa09374 XM_005164746.4
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43507 XM_009300285.3
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43508 XM_009300286.3
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43509 XM_009300287.3
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43510 XM_009300288.3
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa43507 XM_009300285.2 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa09374 XM_005164746.3 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43510 XM_009300288.2 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43508 XM_009300286.2 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43509 XM_009300287.2 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa00690
    Clone ID Related Accession (Same CDS sequence) NM_001044960.2
    Accession Version NM_001044960.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX323820.4. On Mar 1, 2008 this sequence version replaced NM_001044960.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGAA CCTGGGTCCG
    AGTCAGATGA TTTTGGGAGT GATGGCCATA GACGTGTCTC TGGACGACAT CAAGAGACTG
    ACTCCACATT ACACTCTTGG GCCAAATGGA TATTACTTCG CCATTGATCC CAACGGCTAC
    GTTCTACTTC ATCCCAACCT TCAACCCAAG GATCTCAAGT CTCAGGAACC TGTGACGCTG
    GACTTCTTGG ATGCTGAGAT GGAGGATGAA GTCAAAGTGG ATATCAGAAG AAGCATGATA
    GATGGTGAAA GAAGTCAAAT ACGCTTCCAC ACACTTATGA AGTCACAGGA TGAGCGTTAC
    ATAGACAAGG GCTTCAGGAC GTACACTTGG TCTCCAGTCA CTGACGCAGA TTACAGCCTT
    GCGTTAGTTC TGCCAGATTA CAGCATGAGC TACATCAAGG CCACTATAGG AGACACCATC
    ACTCAGGCCA AATTTTCTGA AAGCCTGCAG GTGGACAAGT TCGAGGAGTA TGGATATACT
    TTAATAGCAC CCAGAGATTA CTGTGAAGAC CTGAAGGCTC AGAATGAAAA CATGACACAG
    TTCCTCATCG ACTTCAACGA GTACATCGAC GTAAAGACCC CCAATGACCC ACAGTGCAAT
    ATGGACTTAA TGAATCGACT ACTGTTGGAT GCTGGCATCA CGGCTGAACT GGTCAAACAC
    TGGAACAAAA ATCAATTGAG TGACAGAATC AAGGCTAAGT TTGTGGCGAC AGATGGTGGT
    GTCACTAGAG TATATCCTGC AAGTGCTGGT TCAGAATGGA CCGGACAGGC CGAAACATAT
    GAGTCAAGTT TCTACAAGAG AAGCTTGGAC AATGATCTCT ATGTATTTAC ACCACTGCCA
    TTCACAGGAA ATAAAACCGA AGAGTCAGTT TTAGTGAGCA GAGCAATGGA CTTGAGGATC
    GATGAATTCT GGCTAAAACC AGCAGTTGTC GGTGTGAAAC TGGATGTTCA ATCCTGGATG
    GAAAGCTTCA TGAATACTAC AATTACGCCC AAATGTGAAG GTGAAATATG TGGATGCAGG
    AGGAACGATG AGTATCTGGA CTGTGTTCTT CTAGACGATG GAGGATTCCT TGTCATGGCC
    AATCAGGACA AATACATTGA CCAGATTGGA GAGTTTTTCG GCATGATTGA CCCCAGCCTA
    ATGAGAAATC TGATGAACGT GTCCCTGTTT TCAGTCAACA TAACCTACGA CTACCAGGCC
    CTTTGCGAAC CCACCAGAGA ATCTAAAGCT GCCGCTGGTC TCAGATCAGT CTATGTGCCC
    ACCATAGCTG ATATCCTGAA CATTGGTTGG TGGGCTTCTG CAGCAGCCTG GTCGATAATG
    CAGCAGTTGT TTGTCAGCAT TACATTTCCT AATTTCCTTG AGGCAGTGGA CATAAGTGAT
    GAGTCTGAAA CACCATCTAA AGAGAGTTGC ATTAAAGAGC AGACGCAGTA TTTCTTTGAC
    AGCAATGACA CAACTTTTAA GGGGACTGTT GATTGCCAGA ACTGCTCCAG GCAGTTTATA
    GCTGAAAAGC TTATGAAGAC AAATCTGGTG CTTATTGTTG CTGATACAGA GATGTGCAGC
    CAGATGTCTC CTGGTCCAGA TCCCTGCTTG ATATCGCAGA ACCCACGGTA TCGGAAAGGT
    CCAATGGAGT GCTTTGACAA CAATGTCAAT GAAAATAATG AGCCATGTGG AGGGGCAACA
    AGTCTGACTC CATCTCTCGT GTCCATGGGC ATCCTCCAGC TGCTGCTGCT GCTCTGGCTT
    CTGATTGGAC GAGAACACTG CCTCTCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001038425.2
    CDS96..3305
    Translation

    Target ORF information:

    RefSeq Version NM_001044960.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001044960.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGAA CCTGGGTCCG
    AGTCAGATGA TTTTGGGAGT GATGGCCATA GACGTGTCTC TGGACGACAT CAAGAGACTG
    ACTCCACATT ACACTCTTGG GCCAAATGGA TATTACTTCG CCATTGATCC CAACGGCTAC
    GTTCTACTTC ATCCCAACCT TCAACCCAAG GATCTCAAGT CTCAGGAACC TGTGACGCTG
    GACTTCTTGG ATGCTGAGAT GGAGGATGAA GTCAAAGTGG ATATCAGAAG AAGCATGATA
    GATGGTGAAA GAAGTCAAAT ACGCTTCCAC ACACTTATGA AGTCACAGGA TGAGCGTTAC
    ATAGACAAGG GCTTCAGGAC GTACACTTGG TCTCCAGTCA CTGACGCAGA TTACAGCCTT
    GCGTTAGTTC TGCCAGATTA CAGCATGAGC TACATCAAGG CCACTATAGG AGACACCATC
    ACTCAGGCCA AATTTTCTGA AAGCCTGCAG GTGGACAAGT TCGAGGAGTA TGGATATACT
    TTAATAGCAC CCAGAGATTA CTGTGAAGAC CTGAAGGCTC AGAATGAAAA CATGACACAG
    TTCCTCATCG ACTTCAACGA GTACATCGAC GTAAAGACCC CCAATGACCC ACAGTGCAAT
    ATGGACTTAA TGAATCGACT ACTGTTGGAT GCTGGCATCA CGGCTGAACT GGTCAAACAC
    TGGAACAAAA ATCAATTGAG TGACAGAATC AAGGCTAAGT TTGTGGCGAC AGATGGTGGT
    GTCACTAGAG TATATCCTGC AAGTGCTGGT TCAGAATGGA CCGGACAGGC CGAAACATAT
    GAGTCAAGTT TCTACAAGAG AAGCTTGGAC AATGATCTCT ATGTATTTAC ACCACTGCCA
    TTCACAGGAA ATAAAACCGA AGAGTCAGTT TTAGTGAGCA GAGCAATGGA CTTGAGGATC
    GATGAATTCT GGCTAAAACC AGCAGTTGTC GGTGTGAAAC TGGATGTTCA ATCCTGGATG
    GAAAGCTTCA TGAATACTAC AATTACGCCC AAATGTGAAG GTGAAATATG TGGATGCAGG
    AGGAACGATG AGTATCTGGA CTGTGTTCTT CTAGACGATG GAGGATTCCT TGTCATGGCC
    AATCAGGACA AATACATTGA CCAGATTGGA GAGTTTTTCG GCATGATTGA CCCCAGCCTA
    ATGAGAAATC TGATGAACGT GTCCCTGTTT TCAGTCAACA TAACCTACGA CTACCAGGCC
    CTTTGCGAAC CCACCAGAGA ATCTAAAGCT GCCGCTGGTC TCAGATCAGT CTATGTGCCC
    ACCATAGCTG ATATCCTGAA CATTGGTTGG TGGGCTTCTG CAGCAGCCTG GTCGATAATG
    CAGCAGTTGT TTGTCAGCAT TACATTTCCT AATTTCCTTG AGGCAGTGGA CATAAGTGAT
    GAGTCTGAAA CACCATCTAA AGAGAGTTGC ATTAAAGAGC AGACGCAGTA TTTCTTTGAC
    AGCAATGACA CAACTTTTAA GGGGACTGTT GATTGCCAGA ACTGCTCCAG GCAGTTTATA
    GCTGAAAAGC TTATGAAGAC AAATCTGGTG CTTATTGTTG CTGATACAGA GATGTGCAGC
    CAGATGTCTC CTGGTCCAGA TCCCTGCTTG ATATCGCAGA ACCCACGGTA TCGGAAAGGT
    CCAATGGAGT GCTTTGACAA CAATGTCAAT GAAAATAATG AGCCATGTGG AGGGGCAACA
    AGTCTGACTC CATCTCTCGT GTCCATGGGC ATCCTCCAGC TGCTGCTGCT GCTCTGGCTT
    CTGATTGGAC GAGAACACTG CCTCTCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09374
    Clone ID Related Accession (Same CDS sequence) XM_005164746.4 , XM_005164746.3
    Accession Version XM_005164746.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164746.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATT TTCTGAAAGC CTGCAGGTGG ACAAGTTCGA GGAGTATGGA
    TATACTTTAA TAGCACCCAG AGATTACTGT GAAGACCTGA AGGCTCAGAA TGAAAACATG
    ACACAGTTCC TCATCGACTT CAACGAGTAC ATCGACGTAA AGACCCCCAA TGACCCACAG
    TGCAATATGG ACTTAATGAA TCGACTACTG TTGGATGCTG GCATCACGGC TGAACTGGTC
    AAACACTGGA ACAAAAATCA ATTGAGTGAC AGAATCAAGG CTAAGTTTGT GGCGACAGAT
    GGTGGTGTCA CTAGAGTATA TCCTGCAAGT GCTGGTTCAG AATGGACCGG ACAGGCCGAA
    ACATATGAGT CAAGTTTCTA CAAGAGAAGC TTGGACAATG ATCTCTATGT ATTTACACCA
    CTGCCATTCA CAGGAAATAA AACCGAAGAG TCAGTTTTAG TGAGCAGAGC AATGGACTTG
    AGGATCGATG AATTCTGGCT AAAACCAGCA GTTGTCGGTG TGAAACTGGA TGTTCAATCC
    TGGATGGAAA GCTTCATGAA TACTACAATT ACGCCCAAAT GTGAAGGTGA AATATGTGGA
    TGCAGGAGGA ACGATGAGTA TCTGGACTGT GTTCTTCTAG ACGATGGAGG ATTCCTTGTC
    ATGGCCAATC AGGACAAATA CATTGACCAG ATTGGAGAGT TTTTCGGCAT GATTGACCCC
    AGCCTAATGA GAAATCTGAT GAACGTGTCC CTGTTTTCAG TCAACATAAC CTACGACTAC
    CAGGCCCTTT GCGAACCCAC CAGAGAATCT AAAGCTGCCG CTGGTCTCAG ATCAGTCTAT
    GTGCCCACCA TAGCTGATAT CCTGAACATT GGTTGGTGGG CTTCTGCAGC AGCCTGGTCG
    ATAATGCAGC AGTTGTTTGT CAGCATTACA TTTCCTAATT TCCTTGAGGC AGTGGACATA
    AGTGATGAGT CTGAAACACC ATCTAAAGAG AGTTGCATTA AAGAGCAGAC GCAGTATTTC
    TTTGACAGCA ATGACACAAC TTTTAAGGGG ACTGTTGATT GCCAGAACTG CTCCAGGCAG
    TTTATAGCTG AAAAGCTTAT GAAGACAAAT CTGGTGCTTA TTGTTGCTGA TACAGAGATG
    TGCAGCCAGA TGTGTGAGGA AAAACCATTT TCCCAGGAAG AAAAGCCTTC TCCTGGTCCA
    GATCCCTGCT TGATATCGCA GAACCCACGG TATCGGAAAG GTCCAATGGA GTGCTTTGAC
    AACAATGTCA ATGAAAATAA TGAGCCATGT GGAGGGGCAA CAAGTCTGAC TCCATCTCTC
    GTGTCCATGG GCATCCTCCA GCTGCTGCTG CTGCTCTGGC TTCTGATTGG ACGAGAACAC
    TGCCTCTCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164803.1
    CDS266..3517
    Translation

    Target ORF information:

    RefSeq Version XM_005164746.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164746.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATT TTCTGAAAGC CTGCAGGTGG ACAAGTTCGA GGAGTATGGA
    TATACTTTAA TAGCACCCAG AGATTACTGT GAAGACCTGA AGGCTCAGAA TGAAAACATG
    ACACAGTTCC TCATCGACTT CAACGAGTAC ATCGACGTAA AGACCCCCAA TGACCCACAG
    TGCAATATGG ACTTAATGAA TCGACTACTG TTGGATGCTG GCATCACGGC TGAACTGGTC
    AAACACTGGA ACAAAAATCA ATTGAGTGAC AGAATCAAGG CTAAGTTTGT GGCGACAGAT
    GGTGGTGTCA CTAGAGTATA TCCTGCAAGT GCTGGTTCAG AATGGACCGG ACAGGCCGAA
    ACATATGAGT CAAGTTTCTA CAAGAGAAGC TTGGACAATG ATCTCTATGT ATTTACACCA
    CTGCCATTCA CAGGAAATAA AACCGAAGAG TCAGTTTTAG TGAGCAGAGC AATGGACTTG
    AGGATCGATG AATTCTGGCT AAAACCAGCA GTTGTCGGTG TGAAACTGGA TGTTCAATCC
    TGGATGGAAA GCTTCATGAA TACTACAATT ACGCCCAAAT GTGAAGGTGA AATATGTGGA
    TGCAGGAGGA ACGATGAGTA TCTGGACTGT GTTCTTCTAG ACGATGGAGG ATTCCTTGTC
    ATGGCCAATC AGGACAAATA CATTGACCAG ATTGGAGAGT TTTTCGGCAT GATTGACCCC
    AGCCTAATGA GAAATCTGAT GAACGTGTCC CTGTTTTCAG TCAACATAAC CTACGACTAC
    CAGGCCCTTT GCGAACCCAC CAGAGAATCT AAAGCTGCCG CTGGTCTCAG ATCAGTCTAT
    GTGCCCACCA TAGCTGATAT CCTGAACATT GGTTGGTGGG CTTCTGCAGC AGCCTGGTCG
    ATAATGCAGC AGTTGTTTGT CAGCATTACA TTTCCTAATT TCCTTGAGGC AGTGGACATA
    AGTGATGAGT CTGAAACACC ATCTAAAGAG AGTTGCATTA AAGAGCAGAC GCAGTATTTC
    TTTGACAGCA ATGACACAAC TTTTAAGGGG ACTGTTGATT GCCAGAACTG CTCCAGGCAG
    TTTATAGCTG AAAAGCTTAT GAAGACAAAT CTGGTGCTTA TTGTTGCTGA TACAGAGATG
    TGCAGCCAGA TGTGTGAGGA AAAACCATTT TCCCAGGAAG AAAAGCCTTC TCCTGGTCCA
    GATCCCTGCT TGATATCGCA GAACCCACGG TATCGGAAAG GTCCAATGGA GTGCTTTGAC
    AACAATGTCA ATGAAAATAA TGAGCCATGT GGAGGGGCAA CAAGTCTGAC TCCATCTCTC
    GTGTCCATGG GCATCCTCCA GCTGCTGCTG CTGCTCTGGC TTCTGATTGG ACGAGAACAC
    TGCCTCTCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09374
    Clone ID Related Accession (Same CDS sequence) XM_005164746.4 , XM_005164746.3
    Accession Version XM_005164746.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATT TTCTGAAAGC CTGCAGGTGG ACAAGTTCGA GGAGTATGGA
    TATACTTTAA TAGCACCCAG AGATTACTGT GAAGACCTGA AGGCTCAGAA TGAAAACATG
    ACACAGTTCC TCATCGACTT CAACGAGTAC ATCGACGTAA AGACCCCCAA TGACCCACAG
    TGCAATATGG ACTTAATGAA TCGACTACTG TTGGATGCTG GCATCACGGC TGAACTGGTC
    AAACACTGGA ACAAAAATCA ATTGAGTGAC AGAATCAAGG CTAAGTTTGT GGCGACAGAT
    GGTGGTGTCA CTAGAGTATA TCCTGCAAGT GCTGGTTCAG AATGGACCGG ACAGGCCGAA
    ACATATGAGT CAAGTTTCTA CAAGAGAAGC TTGGACAATG ATCTCTATGT ATTTACACCA
    CTGCCATTCA CAGGAAATAA AACCGAAGAG TCAGTTTTAG TGAGCAGAGC AATGGACTTG
    AGGATCGATG AATTCTGGCT AAAACCAGCA GTTGTCGGTG TGAAACTGGA TGTTCAATCC
    TGGATGGAAA GCTTCATGAA TACTACAATT ACGCCCAAAT GTGAAGGTGA AATATGTGGA
    TGCAGGAGGA ACGATGAGTA TCTGGACTGT GTTCTTCTAG ACGATGGAGG ATTCCTTGTC
    ATGGCCAATC AGGACAAATA CATTGACCAG ATTGGAGAGT TTTTCGGCAT GATTGACCCC
    AGCCTAATGA GAAATCTGAT GAACGTGTCC CTGTTTTCAG TCAACATAAC CTACGACTAC
    CAGGCCCTTT GCGAACCCAC CAGAGAATCT AAAGCTGCCG CTGGTCTCAG ATCAGTCTAT
    GTGCCCACCA TAGCTGATAT CCTGAACATT GGTTGGTGGG CTTCTGCAGC AGCCTGGTCG
    ATAATGCAGC AGTTGTTTGT CAGCATTACA TTTCCTAATT TCCTTGAGGC AGTGGACATA
    AGTGATGAGT CTGAAACACC ATCTAAAGAG AGTTGCATTA AAGAGCAGAC GCAGTATTTC
    TTTGACAGCA ATGACACAAC TTTTAAGGGG ACTGTTGATT GCCAGAACTG CTCCAGGCAG
    TTTATAGCTG AAAAGCTTAT GAAGACAAAT CTGGTGCTTA TTGTTGCTGA TACAGAGATG
    TGCAGCCAGA TGTGTGAGGA AAAACCATTT TCCCAGGAAG AAAAGCCTTC TCCTGGTCCA
    GATCCCTGCT TGATATCGCA GAACCCACGG TATCGGAAAG GTCCAATGGA GTGCTTTGAC
    AACAATGTCA ATGAAAATAA TGAGCCATGT GGAGGGGCAA CAAGTCTGAC TCCATCTCTC
    GTGTCCATGG GCATCCTCCA GCTGCTGCTG CTGCTCTGGC TTCTGATTGG ACGAGAACAC
    TGCCTCTCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164803.1
    CDS275..3526
    Translation

    Target ORF information:

    RefSeq Version XM_005164746.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164746.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATT TTCTGAAAGC CTGCAGGTGG ACAAGTTCGA GGAGTATGGA
    TATACTTTAA TAGCACCCAG AGATTACTGT GAAGACCTGA AGGCTCAGAA TGAAAACATG
    ACACAGTTCC TCATCGACTT CAACGAGTAC ATCGACGTAA AGACCCCCAA TGACCCACAG
    TGCAATATGG ACTTAATGAA TCGACTACTG TTGGATGCTG GCATCACGGC TGAACTGGTC
    AAACACTGGA ACAAAAATCA ATTGAGTGAC AGAATCAAGG CTAAGTTTGT GGCGACAGAT
    GGTGGTGTCA CTAGAGTATA TCCTGCAAGT GCTGGTTCAG AATGGACCGG ACAGGCCGAA
    ACATATGAGT CAAGTTTCTA CAAGAGAAGC TTGGACAATG ATCTCTATGT ATTTACACCA
    CTGCCATTCA CAGGAAATAA AACCGAAGAG TCAGTTTTAG TGAGCAGAGC AATGGACTTG
    AGGATCGATG AATTCTGGCT AAAACCAGCA GTTGTCGGTG TGAAACTGGA TGTTCAATCC
    TGGATGGAAA GCTTCATGAA TACTACAATT ACGCCCAAAT GTGAAGGTGA AATATGTGGA
    TGCAGGAGGA ACGATGAGTA TCTGGACTGT GTTCTTCTAG ACGATGGAGG ATTCCTTGTC
    ATGGCCAATC AGGACAAATA CATTGACCAG ATTGGAGAGT TTTTCGGCAT GATTGACCCC
    AGCCTAATGA GAAATCTGAT GAACGTGTCC CTGTTTTCAG TCAACATAAC CTACGACTAC
    CAGGCCCTTT GCGAACCCAC CAGAGAATCT AAAGCTGCCG CTGGTCTCAG ATCAGTCTAT
    GTGCCCACCA TAGCTGATAT CCTGAACATT GGTTGGTGGG CTTCTGCAGC AGCCTGGTCG
    ATAATGCAGC AGTTGTTTGT CAGCATTACA TTTCCTAATT TCCTTGAGGC AGTGGACATA
    AGTGATGAGT CTGAAACACC ATCTAAAGAG AGTTGCATTA AAGAGCAGAC GCAGTATTTC
    TTTGACAGCA ATGACACAAC TTTTAAGGGG ACTGTTGATT GCCAGAACTG CTCCAGGCAG
    TTTATAGCTG AAAAGCTTAT GAAGACAAAT CTGGTGCTTA TTGTTGCTGA TACAGAGATG
    TGCAGCCAGA TGTGTGAGGA AAAACCATTT TCCCAGGAAG AAAAGCCTTC TCCTGGTCCA
    GATCCCTGCT TGATATCGCA GAACCCACGG TATCGGAAAG GTCCAATGGA GTGCTTTGAC
    AACAATGTCA ATGAAAATAA TGAGCCATGT GGAGGGGCAA CAAGTCTGAC TCCATCTCTC
    GTGTCCATGG GCATCCTCCA GCTGCTGCTG CTGCTCTGGC TTCTGATTGG ACGAGAACAC
    TGCCTCTCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43507
    Clone ID Related Accession (Same CDS sequence) XM_009300285.3 , XM_009300285.2
    Accession Version XM_009300285.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300285.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG CAGTTTCTGA AAGCCTGCAG
    GTGGACAAGT TCGAGGAGTA TGGATATACT TTAATAGCAC CCAGAGATTA CTGTGAAGAC
    CTGAAGGCTC AGAATGAAAA CATGACACAG TTCCTCATCG ACTTCAACGA GTACATCGAC
    GTAAAGACCC CCAATGACCC ACAGTGCAAT ATGGACTTAA TGAATCGACT ACTGTTGGAT
    GCTGGCATCA CGGCTGAACT GGTCAAACAC TGGAACAAAA ATCAATTGAG TGACAGAATC
    AAGGCTAAGT TTGTGGCGAC AGATGGTGGT GTCACTAGAG TATATCCTGC AAGTGCTGGT
    TCAGAATGGA CCGGACAGGC CGAAACATAT GAGTCAAGTT TCTACAAGAG AAGCTTGGAC
    AATGATCTCT ATGTATTTAC ACCACTGCCA TTCACAGGAA ATAAAACCGA AGAGTCAGTT
    TTAGTGAGCA GAGCAATGGA CTTGAGGATC GATGAATTCT GGCTAAAACC AGCAGTTGTC
    GGTGTGAAAC TGGATGTTCA ATCCTGGATG GAAAGCTTCA TGAATACTAC AATTACGCCC
    AAATGTGAAG GTGAAATATG TGGATGCAGG AGGAACGATG AGTATCTGGA CTGTGTTCTT
    CTAGACGATG GAGGATTCCT TGTCATGGCC AATCAGGACA AATACATTGA CCAGATTGGA
    GAGTTTTTCG GCATGATTGA CCCCAGCCTA ATGAGAAATC TGATGAACGT GTCCCTGTTT
    TCAGTCAACA TAACCTACGA CTACCAGGCC CTTTGCGAAC CCACCAGAGA ATCTAAAGCT
    GCCGCTGGTC TCAGATCAGT CTATGTGCCC ACCATAGCTG ATATCCTGAA CATTGGTTGG
    TGGGCTTCTG CAGCAGCCTG GTCGATAATG CAGCAGTTGT TTGTCAGCAT TACATTTCCT
    AATTTCCTTG AGGCAGTGGA CATAAGTGAT GAGTCTGAAA CACCATCTAA AGAGAGTTGC
    ATTAAAGAGC AGACGCAGTA TTTCTTTGAC AGCAATGACA CAACTTTTAA GGGGACTGTT
    GATTGCCAGA ACTGCTCCAG GCAGTTTATA GCTGAAAAGC TTATGAAGAC AAATCTGGTG
    CTTATTGTTG CTGATACAGA GATGTGCAGC CAGATGTGTG AGGAAAAACC ATTTTCCCAG
    GAAGAAAAGC CTTCTCCTGG TCCAGATCCC TGCTTGATAT CGCAGAACCC ACGGTATCGG
    AAAGGTCCAA TGGAGTGCTT TGACAACAAT GTCAATGATG AGGCCATGTG TAGGAGAAAT
    GCAGGCTCTC CACTGCTCTC CTCTCTCCTC ATGTTACTGC TGCCAGTGTT CATGTGCTGT
    CTCGGGAAAA TAATGAGCCA TGTGGAGGGG CAACAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298560.1
    CDS268..3549
    Translation

    Target ORF information:

    RefSeq Version XM_009300285.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300285.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG CAGTTTCTGA AAGCCTGCAG
    GTGGACAAGT TCGAGGAGTA TGGATATACT TTAATAGCAC CCAGAGATTA CTGTGAAGAC
    CTGAAGGCTC AGAATGAAAA CATGACACAG TTCCTCATCG ACTTCAACGA GTACATCGAC
    GTAAAGACCC CCAATGACCC ACAGTGCAAT ATGGACTTAA TGAATCGACT ACTGTTGGAT
    GCTGGCATCA CGGCTGAACT GGTCAAACAC TGGAACAAAA ATCAATTGAG TGACAGAATC
    AAGGCTAAGT TTGTGGCGAC AGATGGTGGT GTCACTAGAG TATATCCTGC AAGTGCTGGT
    TCAGAATGGA CCGGACAGGC CGAAACATAT GAGTCAAGTT TCTACAAGAG AAGCTTGGAC
    AATGATCTCT ATGTATTTAC ACCACTGCCA TTCACAGGAA ATAAAACCGA AGAGTCAGTT
    TTAGTGAGCA GAGCAATGGA CTTGAGGATC GATGAATTCT GGCTAAAACC AGCAGTTGTC
    GGTGTGAAAC TGGATGTTCA ATCCTGGATG GAAAGCTTCA TGAATACTAC AATTACGCCC
    AAATGTGAAG GTGAAATATG TGGATGCAGG AGGAACGATG AGTATCTGGA CTGTGTTCTT
    CTAGACGATG GAGGATTCCT TGTCATGGCC AATCAGGACA AATACATTGA CCAGATTGGA
    GAGTTTTTCG GCATGATTGA CCCCAGCCTA ATGAGAAATC TGATGAACGT GTCCCTGTTT
    TCAGTCAACA TAACCTACGA CTACCAGGCC CTTTGCGAAC CCACCAGAGA ATCTAAAGCT
    GCCGCTGGTC TCAGATCAGT CTATGTGCCC ACCATAGCTG ATATCCTGAA CATTGGTTGG
    TGGGCTTCTG CAGCAGCCTG GTCGATAATG CAGCAGTTGT TTGTCAGCAT TACATTTCCT
    AATTTCCTTG AGGCAGTGGA CATAAGTGAT GAGTCTGAAA CACCATCTAA AGAGAGTTGC
    ATTAAAGAGC AGACGCAGTA TTTCTTTGAC AGCAATGACA CAACTTTTAA GGGGACTGTT
    GATTGCCAGA ACTGCTCCAG GCAGTTTATA GCTGAAAAGC TTATGAAGAC AAATCTGGTG
    CTTATTGTTG CTGATACAGA GATGTGCAGC CAGATGTGTG AGGAAAAACC ATTTTCCCAG
    GAAGAAAAGC CTTCTCCTGG TCCAGATCCC TGCTTGATAT CGCAGAACCC ACGGTATCGG
    AAAGGTCCAA TGGAGTGCTT TGACAACAAT GTCAATGATG AGGCCATGTG TAGGAGAAAT
    GCAGGCTCTC CACTGCTCTC CTCTCTCCTC ATGTTACTGC TGCCAGTGTT CATGTGCTGT
    CTCGGGAAAA TAATGAGCCA TGTGGAGGGG CAACAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43507
    Clone ID Related Accession (Same CDS sequence) XM_009300285.3 , XM_009300285.2
    Accession Version XM_009300285.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG CAGTTTCTGA AAGCCTGCAG
    GTGGACAAGT TCGAGGAGTA TGGATATACT TTAATAGCAC CCAGAGATTA CTGTGAAGAC
    CTGAAGGCTC AGAATGAAAA CATGACACAG TTCCTCATCG ACTTCAACGA GTACATCGAC
    GTAAAGACCC CCAATGACCC ACAGTGCAAT ATGGACTTAA TGAATCGACT ACTGTTGGAT
    GCTGGCATCA CGGCTGAACT GGTCAAACAC TGGAACAAAA ATCAATTGAG TGACAGAATC
    AAGGCTAAGT TTGTGGCGAC AGATGGTGGT GTCACTAGAG TATATCCTGC AAGTGCTGGT
    TCAGAATGGA CCGGACAGGC CGAAACATAT GAGTCAAGTT TCTACAAGAG AAGCTTGGAC
    AATGATCTCT ATGTATTTAC ACCACTGCCA TTCACAGGAA ATAAAACCGA AGAGTCAGTT
    TTAGTGAGCA GAGCAATGGA CTTGAGGATC GATGAATTCT GGCTAAAACC AGCAGTTGTC
    GGTGTGAAAC TGGATGTTCA ATCCTGGATG GAAAGCTTCA TGAATACTAC AATTACGCCC
    AAATGTGAAG GTGAAATATG TGGATGCAGG AGGAACGATG AGTATCTGGA CTGTGTTCTT
    CTAGACGATG GAGGATTCCT TGTCATGGCC AATCAGGACA AATACATTGA CCAGATTGGA
    GAGTTTTTCG GCATGATTGA CCCCAGCCTA ATGAGAAATC TGATGAACGT GTCCCTGTTT
    TCAGTCAACA TAACCTACGA CTACCAGGCC CTTTGCGAAC CCACCAGAGA ATCTAAAGCT
    GCCGCTGGTC TCAGATCAGT CTATGTGCCC ACCATAGCTG ATATCCTGAA CATTGGTTGG
    TGGGCTTCTG CAGCAGCCTG GTCGATAATG CAGCAGTTGT TTGTCAGCAT TACATTTCCT
    AATTTCCTTG AGGCAGTGGA CATAAGTGAT GAGTCTGAAA CACCATCTAA AGAGAGTTGC
    ATTAAAGAGC AGACGCAGTA TTTCTTTGAC AGCAATGACA CAACTTTTAA GGGGACTGTT
    GATTGCCAGA ACTGCTCCAG GCAGTTTATA GCTGAAAAGC TTATGAAGAC AAATCTGGTG
    CTTATTGTTG CTGATACAGA GATGTGCAGC CAGATGTGTG AGGAAAAACC ATTTTCCCAG
    GAAGAAAAGC CTTCTCCTGG TCCAGATCCC TGCTTGATAT CGCAGAACCC ACGGTATCGG
    AAAGGTCCAA TGGAGTGCTT TGACAACAAT GTCAATGATG AGGCCATGTG TAGGAGAAAT
    GCAGGCTCTC CACTGCTCTC CTCTCTCCTC ATGTTACTGC TGCCAGTGTT CATGTGCTGT
    CTCGGGAAAA TAATGAGCCA TGTGGAGGGG CAACAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298560.1
    CDS277..3558
    Translation

    Target ORF information:

    RefSeq Version XM_009300285.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG CAGTTTCTGA AAGCCTGCAG
    GTGGACAAGT TCGAGGAGTA TGGATATACT TTAATAGCAC CCAGAGATTA CTGTGAAGAC
    CTGAAGGCTC AGAATGAAAA CATGACACAG TTCCTCATCG ACTTCAACGA GTACATCGAC
    GTAAAGACCC CCAATGACCC ACAGTGCAAT ATGGACTTAA TGAATCGACT ACTGTTGGAT
    GCTGGCATCA CGGCTGAACT GGTCAAACAC TGGAACAAAA ATCAATTGAG TGACAGAATC
    AAGGCTAAGT TTGTGGCGAC AGATGGTGGT GTCACTAGAG TATATCCTGC AAGTGCTGGT
    TCAGAATGGA CCGGACAGGC CGAAACATAT GAGTCAAGTT TCTACAAGAG AAGCTTGGAC
    AATGATCTCT ATGTATTTAC ACCACTGCCA TTCACAGGAA ATAAAACCGA AGAGTCAGTT
    TTAGTGAGCA GAGCAATGGA CTTGAGGATC GATGAATTCT GGCTAAAACC AGCAGTTGTC
    GGTGTGAAAC TGGATGTTCA ATCCTGGATG GAAAGCTTCA TGAATACTAC AATTACGCCC
    AAATGTGAAG GTGAAATATG TGGATGCAGG AGGAACGATG AGTATCTGGA CTGTGTTCTT
    CTAGACGATG GAGGATTCCT TGTCATGGCC AATCAGGACA AATACATTGA CCAGATTGGA
    GAGTTTTTCG GCATGATTGA CCCCAGCCTA ATGAGAAATC TGATGAACGT GTCCCTGTTT
    TCAGTCAACA TAACCTACGA CTACCAGGCC CTTTGCGAAC CCACCAGAGA ATCTAAAGCT
    GCCGCTGGTC TCAGATCAGT CTATGTGCCC ACCATAGCTG ATATCCTGAA CATTGGTTGG
    TGGGCTTCTG CAGCAGCCTG GTCGATAATG CAGCAGTTGT TTGTCAGCAT TACATTTCCT
    AATTTCCTTG AGGCAGTGGA CATAAGTGAT GAGTCTGAAA CACCATCTAA AGAGAGTTGC
    ATTAAAGAGC AGACGCAGTA TTTCTTTGAC AGCAATGACA CAACTTTTAA GGGGACTGTT
    GATTGCCAGA ACTGCTCCAG GCAGTTTATA GCTGAAAAGC TTATGAAGAC AAATCTGGTG
    CTTATTGTTG CTGATACAGA GATGTGCAGC CAGATGTGTG AGGAAAAACC ATTTTCCCAG
    GAAGAAAAGC CTTCTCCTGG TCCAGATCCC TGCTTGATAT CGCAGAACCC ACGGTATCGG
    AAAGGTCCAA TGGAGTGCTT TGACAACAAT GTCAATGATG AGGCCATGTG TAGGAGAAAT
    GCAGGCTCTC CACTGCTCTC CTCTCTCCTC ATGTTACTGC TGCCAGTGTT CATGTGCTGT
    CTCGGGAAAA TAATGAGCCA TGTGGAGGGG CAACAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43508
    Clone ID Related Accession (Same CDS sequence) XM_009300286.2 , XM_009300286.3
    Accession Version XM_009300286.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG TTTCTGAAAG CCTGCAGGTG
    GACAAGTTCG AGGAGTATGG ATATACTTTA ATAGCACCCA GAGATTACTG TGAAGACCTG
    AAGGCTCAGA ATGAAAACAT GACACAGTTC CTCATCGACT TCAACGAGTA CATCGACGTA
    AAGACCCCCA ATGACCCACA GTGCAATATG GACTTAATGA ATCGACTACT GTTGGATGCT
    GGCATCACGG CTGAACTGGT CAAACACTGG AACAAAAATC AATTGAGTGA CAGAATCAAG
    GCTAAGTTTG TGGCGACAGA TGGTGGTGTC ACTAGAGTAT ATCCTGCAAG TGCTGGTTCA
    GAATGGACCG GACAGGCCGA AACATATGAG TCAAGTTTCT ACAAGAGAAG CTTGGACAAT
    GATCTCTATG TATTTACACC ACTGCCATTC ACAGGAAATA AAACCGAAGA GTCAGTTTTA
    GTGAGCAGAG CAATGGACTT GAGGATCGAT GAATTCTGGC TAAAACCAGC AGTTGTCGGT
    GTGAAACTGG ATGTTCAATC CTGGATGGAA AGCTTCATGA ATACTACAAT TACGCCCAAA
    TGTGAAGGTG AAATATGTGG ATGCAGGAGG AACGATGAGT ATCTGGACTG TGTTCTTCTA
    GACGATGGAG GATTCCTTGT CATGGCCAAT CAGGACAAAT ACATTGACCA GATTGGAGAG
    TTTTTCGGCA TGATTGACCC CAGCCTAATG AGAAATCTGA TGAACGTGTC CCTGTTTTCA
    GTCAACATAA CCTACGACTA CCAGGCCCTT TGCGAACCCA CCAGAGAATC TAAAGCTGCC
    GCTGGTCTCA GATCAGTCTA TGTGCCCACC ATAGCTGATA TCCTGAACAT TGGTTGGTGG
    GCTTCTGCAG CAGCCTGGTC GATAATGCAG CAGTTGTTTG TCAGCATTAC ATTTCCTAAT
    TTCCTTGAGG CAGTGGACAT AAGTGATGAG TCTGAAACAC CATCTAAAGA GAGTTGCATT
    AAAGAGCAGA CGCAGTATTT CTTTGACAGC AATGACACAA CTTTTAAGGG GACTGTTGAT
    TGCCAGAACT GCTCCAGGCA GTTTATAGCT GAAAAGCTTA TGAAGACAAA TCTGGTGCTT
    ATTGTTGCTG ATACAGAGAT GTGCAGCCAG ATGTGTGAGG AAAAACCATT TTCCCAGGAA
    GAAAAGCCTT CTCCTGGTCC AGATCCCTGC TTGATATCGC AGAACCCACG GTATCGGAAA
    GGTCCAATGG AGTGCTTTGA CAACAATGTC AATGATGAGG CCATGTGTAG GAGAAATGCA
    GGCTCTCCAC TGCTCTCCTC TCTCCTCATG TTACTGCTGC CAGTGTTCAT GTGCTGTCTC
    GGGAAAATAA TGAGCCATGT GGAGGGGCAA CAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298561.1
    CDS277..3555
    Translation

    Target ORF information:

    RefSeq Version XM_009300286.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG TTTCTGAAAG CCTGCAGGTG
    GACAAGTTCG AGGAGTATGG ATATACTTTA ATAGCACCCA GAGATTACTG TGAAGACCTG
    AAGGCTCAGA ATGAAAACAT GACACAGTTC CTCATCGACT TCAACGAGTA CATCGACGTA
    AAGACCCCCA ATGACCCACA GTGCAATATG GACTTAATGA ATCGACTACT GTTGGATGCT
    GGCATCACGG CTGAACTGGT CAAACACTGG AACAAAAATC AATTGAGTGA CAGAATCAAG
    GCTAAGTTTG TGGCGACAGA TGGTGGTGTC ACTAGAGTAT ATCCTGCAAG TGCTGGTTCA
    GAATGGACCG GACAGGCCGA AACATATGAG TCAAGTTTCT ACAAGAGAAG CTTGGACAAT
    GATCTCTATG TATTTACACC ACTGCCATTC ACAGGAAATA AAACCGAAGA GTCAGTTTTA
    GTGAGCAGAG CAATGGACTT GAGGATCGAT GAATTCTGGC TAAAACCAGC AGTTGTCGGT
    GTGAAACTGG ATGTTCAATC CTGGATGGAA AGCTTCATGA ATACTACAAT TACGCCCAAA
    TGTGAAGGTG AAATATGTGG ATGCAGGAGG AACGATGAGT ATCTGGACTG TGTTCTTCTA
    GACGATGGAG GATTCCTTGT CATGGCCAAT CAGGACAAAT ACATTGACCA GATTGGAGAG
    TTTTTCGGCA TGATTGACCC CAGCCTAATG AGAAATCTGA TGAACGTGTC CCTGTTTTCA
    GTCAACATAA CCTACGACTA CCAGGCCCTT TGCGAACCCA CCAGAGAATC TAAAGCTGCC
    GCTGGTCTCA GATCAGTCTA TGTGCCCACC ATAGCTGATA TCCTGAACAT TGGTTGGTGG
    GCTTCTGCAG CAGCCTGGTC GATAATGCAG CAGTTGTTTG TCAGCATTAC ATTTCCTAAT
    TTCCTTGAGG CAGTGGACAT AAGTGATGAG TCTGAAACAC CATCTAAAGA GAGTTGCATT
    AAAGAGCAGA CGCAGTATTT CTTTGACAGC AATGACACAA CTTTTAAGGG GACTGTTGAT
    TGCCAGAACT GCTCCAGGCA GTTTATAGCT GAAAAGCTTA TGAAGACAAA TCTGGTGCTT
    ATTGTTGCTG ATACAGAGAT GTGCAGCCAG ATGTGTGAGG AAAAACCATT TTCCCAGGAA
    GAAAAGCCTT CTCCTGGTCC AGATCCCTGC TTGATATCGC AGAACCCACG GTATCGGAAA
    GGTCCAATGG AGTGCTTTGA CAACAATGTC AATGATGAGG CCATGTGTAG GAGAAATGCA
    GGCTCTCCAC TGCTCTCCTC TCTCCTCATG TTACTGCTGC CAGTGTTCAT GTGCTGTCTC
    GGGAAAATAA TGAGCCATGT GGAGGGGCAA CAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43508
    Clone ID Related Accession (Same CDS sequence) XM_009300286.2 , XM_009300286.3
    Accession Version XM_009300286.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG TTTCTGAAAG CCTGCAGGTG
    GACAAGTTCG AGGAGTATGG ATATACTTTA ATAGCACCCA GAGATTACTG TGAAGACCTG
    AAGGCTCAGA ATGAAAACAT GACACAGTTC CTCATCGACT TCAACGAGTA CATCGACGTA
    AAGACCCCCA ATGACCCACA GTGCAATATG GACTTAATGA ATCGACTACT GTTGGATGCT
    GGCATCACGG CTGAACTGGT CAAACACTGG AACAAAAATC AATTGAGTGA CAGAATCAAG
    GCTAAGTTTG TGGCGACAGA TGGTGGTGTC ACTAGAGTAT ATCCTGCAAG TGCTGGTTCA
    GAATGGACCG GACAGGCCGA AACATATGAG TCAAGTTTCT ACAAGAGAAG CTTGGACAAT
    GATCTCTATG TATTTACACC ACTGCCATTC ACAGGAAATA AAACCGAAGA GTCAGTTTTA
    GTGAGCAGAG CAATGGACTT GAGGATCGAT GAATTCTGGC TAAAACCAGC AGTTGTCGGT
    GTGAAACTGG ATGTTCAATC CTGGATGGAA AGCTTCATGA ATACTACAAT TACGCCCAAA
    TGTGAAGGTG AAATATGTGG ATGCAGGAGG AACGATGAGT ATCTGGACTG TGTTCTTCTA
    GACGATGGAG GATTCCTTGT CATGGCCAAT CAGGACAAAT ACATTGACCA GATTGGAGAG
    TTTTTCGGCA TGATTGACCC CAGCCTAATG AGAAATCTGA TGAACGTGTC CCTGTTTTCA
    GTCAACATAA CCTACGACTA CCAGGCCCTT TGCGAACCCA CCAGAGAATC TAAAGCTGCC
    GCTGGTCTCA GATCAGTCTA TGTGCCCACC ATAGCTGATA TCCTGAACAT TGGTTGGTGG
    GCTTCTGCAG CAGCCTGGTC GATAATGCAG CAGTTGTTTG TCAGCATTAC ATTTCCTAAT
    TTCCTTGAGG CAGTGGACAT AAGTGATGAG TCTGAAACAC CATCTAAAGA GAGTTGCATT
    AAAGAGCAGA CGCAGTATTT CTTTGACAGC AATGACACAA CTTTTAAGGG GACTGTTGAT
    TGCCAGAACT GCTCCAGGCA GTTTATAGCT GAAAAGCTTA TGAAGACAAA TCTGGTGCTT
    ATTGTTGCTG ATACAGAGAT GTGCAGCCAG ATGTGTGAGG AAAAACCATT TTCCCAGGAA
    GAAAAGCCTT CTCCTGGTCC AGATCCCTGC TTGATATCGC AGAACCCACG GTATCGGAAA
    GGTCCAATGG AGTGCTTTGA CAACAATGTC AATGATGAGG CCATGTGTAG GAGAAATGCA
    GGCTCTCCAC TGCTCTCCTC TCTCCTCATG TTACTGCTGC CAGTGTTCAT GTGCTGTCTC
    GGGAAAATAA TGAGCCATGT GGAGGGGCAA CAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298561.1
    CDS268..3546
    Translation

    Target ORF information:

    RefSeq Version XM_009300286.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300286.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG TTTCTGAAAG CCTGCAGGTG
    GACAAGTTCG AGGAGTATGG ATATACTTTA ATAGCACCCA GAGATTACTG TGAAGACCTG
    AAGGCTCAGA ATGAAAACAT GACACAGTTC CTCATCGACT TCAACGAGTA CATCGACGTA
    AAGACCCCCA ATGACCCACA GTGCAATATG GACTTAATGA ATCGACTACT GTTGGATGCT
    GGCATCACGG CTGAACTGGT CAAACACTGG AACAAAAATC AATTGAGTGA CAGAATCAAG
    GCTAAGTTTG TGGCGACAGA TGGTGGTGTC ACTAGAGTAT ATCCTGCAAG TGCTGGTTCA
    GAATGGACCG GACAGGCCGA AACATATGAG TCAAGTTTCT ACAAGAGAAG CTTGGACAAT
    GATCTCTATG TATTTACACC ACTGCCATTC ACAGGAAATA AAACCGAAGA GTCAGTTTTA
    GTGAGCAGAG CAATGGACTT GAGGATCGAT GAATTCTGGC TAAAACCAGC AGTTGTCGGT
    GTGAAACTGG ATGTTCAATC CTGGATGGAA AGCTTCATGA ATACTACAAT TACGCCCAAA
    TGTGAAGGTG AAATATGTGG ATGCAGGAGG AACGATGAGT ATCTGGACTG TGTTCTTCTA
    GACGATGGAG GATTCCTTGT CATGGCCAAT CAGGACAAAT ACATTGACCA GATTGGAGAG
    TTTTTCGGCA TGATTGACCC CAGCCTAATG AGAAATCTGA TGAACGTGTC CCTGTTTTCA
    GTCAACATAA CCTACGACTA CCAGGCCCTT TGCGAACCCA CCAGAGAATC TAAAGCTGCC
    GCTGGTCTCA GATCAGTCTA TGTGCCCACC ATAGCTGATA TCCTGAACAT TGGTTGGTGG
    GCTTCTGCAG CAGCCTGGTC GATAATGCAG CAGTTGTTTG TCAGCATTAC ATTTCCTAAT
    TTCCTTGAGG CAGTGGACAT AAGTGATGAG TCTGAAACAC CATCTAAAGA GAGTTGCATT
    AAAGAGCAGA CGCAGTATTT CTTTGACAGC AATGACACAA CTTTTAAGGG GACTGTTGAT
    TGCCAGAACT GCTCCAGGCA GTTTATAGCT GAAAAGCTTA TGAAGACAAA TCTGGTGCTT
    ATTGTTGCTG ATACAGAGAT GTGCAGCCAG ATGTGTGAGG AAAAACCATT TTCCCAGGAA
    GAAAAGCCTT CTCCTGGTCC AGATCCCTGC TTGATATCGC AGAACCCACG GTATCGGAAA
    GGTCCAATGG AGTGCTTTGA CAACAATGTC AATGATGAGG CCATGTGTAG GAGAAATGCA
    GGCTCTCCAC TGCTCTCCTC TCTCCTCATG TTACTGCTGC CAGTGTTCAT GTGCTGTCTC
    GGGAAAATAA TGAGCCATGT GGAGGGGCAA CAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43509
    Clone ID Related Accession (Same CDS sequence) XM_009300287.2 , XM_009300287.3
    Accession Version XM_009300287.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG CAGTTTCTGA AAGCCTGCAG
    GTGGACAAGT TCGAGGAGTA TGGATATACT TTAATAGCAC CCAGAGATTA CTGTGAAGAC
    CTGAAGGCTC AGAATGAAAA CATGACACAG TTCCTCATCG ACTTCAACGA GTACATCGAC
    GTAAAGACCC CCAATGACCC ACAGTGCAAT ATGGACTTAA TGAATCGACT ACTGTTGGAT
    GCTGGCATCA CGGCTGAACT GGTCAAACAC TGGAACAAAA ATCAATTGAG TGACAGAATC
    AAGGCTAAGT TTGTGGCGAC AGATGGTGGT GTCACTAGAG TATATCCTGC AAGTGCTGGT
    TCAGAATGGA CCGGACAGGC CGAAACATAT GAGTCAAGTT TCTACAAGAG AAGCTTGGAC
    AATGATCTCT ATGTATTTAC ACCACTGCCA TTCACAGGAA ATAAAACCGA AGAGTCAGTT
    TTAGTGAGCA GAGCAATGGA CTTGAGGATC GATGAATTCT GGCTAAAACC AGCAGTTGTC
    GGTGTGAAAC TGGATGTTCA ATCCTGGATG GAAAGCTTCA TGAATACTAC AATTACGCCC
    AAATGTGAAG GTGAAATATG TGGATGCAGG AGGAACGATG AGTATCTGGA CTGTGTTCTT
    CTAGACGATG GAGGATTCCT TGTCATGGCC AATCAGGACA AATACATTGA CCAGATTGGA
    GAGTTTTTCG GCATGATTGA CCCCAGCCTA ATGAGAAATC TGATGAACGT GTCCCTGTTT
    TCAGTCAACA TAACCTACGA CTACCAGGCC CTTTGCGAAC CCACCAGAGA ATCTAAAGCT
    GCCGCTGGTC TCAGATCAGT CTATGTGCCC ACCATAGCTG ATATCCTGAA CATTGGTTGG
    TGGGCTTCTG CAGCAGCCTG GTCGATAATG CAGCAGTTGT TTGTCAGCAT TACATTTCCT
    AATTTCCTTG AGGCAGTGGA CATAAGTGAT GAGTCTGAAA CACCATCTAA AGAGAGTTGC
    ATTAAAGAGC AGACGCAGTA TTTCTTTGAC AGCAATGACA CAACTTTTAA GGGGACTGTT
    GATTGCCAGA ACTGCTCCAG GCAGTTTATA GCTGAAAAGC TTATGAAGAC AAATCTGGTG
    CTTATTGTTG CTGATACAGA GATGTGCAGC CAGATGTGTG AGGAAAAACC ATTTTCCCAG
    GAAGAAAAGC CTTCTCCTGG TCCAGATCCC TGCTTGATAT CGCAGAACCC ACGGTATCGG
    AAAGGTCCAA TGGAGTGCTT TGACAACAAT GTCAATGAAA ATAATGAGCC ATGTGGAGGG
    GCAACAAGTC TGACTCCATC TCTCGTGTCC ATGGGCATCC TCCAGCTGCT GCTGCTGCTC
    TGGCTTCTGA TTGGACGAGA ACACTGCCTC TCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298562.1
    CDS275..3550
    Translation

    Target ORF information:

    RefSeq Version XM_009300287.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300287.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG CAGTTTCTGA AAGCCTGCAG
    GTGGACAAGT TCGAGGAGTA TGGATATACT TTAATAGCAC CCAGAGATTA CTGTGAAGAC
    CTGAAGGCTC AGAATGAAAA CATGACACAG TTCCTCATCG ACTTCAACGA GTACATCGAC
    GTAAAGACCC CCAATGACCC ACAGTGCAAT ATGGACTTAA TGAATCGACT ACTGTTGGAT
    GCTGGCATCA CGGCTGAACT GGTCAAACAC TGGAACAAAA ATCAATTGAG TGACAGAATC
    AAGGCTAAGT TTGTGGCGAC AGATGGTGGT GTCACTAGAG TATATCCTGC AAGTGCTGGT
    TCAGAATGGA CCGGACAGGC CGAAACATAT GAGTCAAGTT TCTACAAGAG AAGCTTGGAC
    AATGATCTCT ATGTATTTAC ACCACTGCCA TTCACAGGAA ATAAAACCGA AGAGTCAGTT
    TTAGTGAGCA GAGCAATGGA CTTGAGGATC GATGAATTCT GGCTAAAACC AGCAGTTGTC
    GGTGTGAAAC TGGATGTTCA ATCCTGGATG GAAAGCTTCA TGAATACTAC AATTACGCCC
    AAATGTGAAG GTGAAATATG TGGATGCAGG AGGAACGATG AGTATCTGGA CTGTGTTCTT
    CTAGACGATG GAGGATTCCT TGTCATGGCC AATCAGGACA AATACATTGA CCAGATTGGA
    GAGTTTTTCG GCATGATTGA CCCCAGCCTA ATGAGAAATC TGATGAACGT GTCCCTGTTT
    TCAGTCAACA TAACCTACGA CTACCAGGCC CTTTGCGAAC CCACCAGAGA ATCTAAAGCT
    GCCGCTGGTC TCAGATCAGT CTATGTGCCC ACCATAGCTG ATATCCTGAA CATTGGTTGG
    TGGGCTTCTG CAGCAGCCTG GTCGATAATG CAGCAGTTGT TTGTCAGCAT TACATTTCCT
    AATTTCCTTG AGGCAGTGGA CATAAGTGAT GAGTCTGAAA CACCATCTAA AGAGAGTTGC
    ATTAAAGAGC AGACGCAGTA TTTCTTTGAC AGCAATGACA CAACTTTTAA GGGGACTGTT
    GATTGCCAGA ACTGCTCCAG GCAGTTTATA GCTGAAAAGC TTATGAAGAC AAATCTGGTG
    CTTATTGTTG CTGATACAGA GATGTGCAGC CAGATGTGTG AGGAAAAACC ATTTTCCCAG
    GAAGAAAAGC CTTCTCCTGG TCCAGATCCC TGCTTGATAT CGCAGAACCC ACGGTATCGG
    AAAGGTCCAA TGGAGTGCTT TGACAACAAT GTCAATGAAA ATAATGAGCC ATGTGGAGGG
    GCAACAAGTC TGACTCCATC TCTCGTGTCC ATGGGCATCC TCCAGCTGCT GCTGCTGCTC
    TGGCTTCTGA TTGGACGAGA ACACTGCCTC TCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43509
    Clone ID Related Accession (Same CDS sequence) XM_009300287.2 , XM_009300287.3
    Accession Version XM_009300287.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300287.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG CAGTTTCTGA AAGCCTGCAG
    GTGGACAAGT TCGAGGAGTA TGGATATACT TTAATAGCAC CCAGAGATTA CTGTGAAGAC
    CTGAAGGCTC AGAATGAAAA CATGACACAG TTCCTCATCG ACTTCAACGA GTACATCGAC
    GTAAAGACCC CCAATGACCC ACAGTGCAAT ATGGACTTAA TGAATCGACT ACTGTTGGAT
    GCTGGCATCA CGGCTGAACT GGTCAAACAC TGGAACAAAA ATCAATTGAG TGACAGAATC
    AAGGCTAAGT TTGTGGCGAC AGATGGTGGT GTCACTAGAG TATATCCTGC AAGTGCTGGT
    TCAGAATGGA CCGGACAGGC CGAAACATAT GAGTCAAGTT TCTACAAGAG AAGCTTGGAC
    AATGATCTCT ATGTATTTAC ACCACTGCCA TTCACAGGAA ATAAAACCGA AGAGTCAGTT
    TTAGTGAGCA GAGCAATGGA CTTGAGGATC GATGAATTCT GGCTAAAACC AGCAGTTGTC
    GGTGTGAAAC TGGATGTTCA ATCCTGGATG GAAAGCTTCA TGAATACTAC AATTACGCCC
    AAATGTGAAG GTGAAATATG TGGATGCAGG AGGAACGATG AGTATCTGGA CTGTGTTCTT
    CTAGACGATG GAGGATTCCT TGTCATGGCC AATCAGGACA AATACATTGA CCAGATTGGA
    GAGTTTTTCG GCATGATTGA CCCCAGCCTA ATGAGAAATC TGATGAACGT GTCCCTGTTT
    TCAGTCAACA TAACCTACGA CTACCAGGCC CTTTGCGAAC CCACCAGAGA ATCTAAAGCT
    GCCGCTGGTC TCAGATCAGT CTATGTGCCC ACCATAGCTG ATATCCTGAA CATTGGTTGG
    TGGGCTTCTG CAGCAGCCTG GTCGATAATG CAGCAGTTGT TTGTCAGCAT TACATTTCCT
    AATTTCCTTG AGGCAGTGGA CATAAGTGAT GAGTCTGAAA CACCATCTAA AGAGAGTTGC
    ATTAAAGAGC AGACGCAGTA TTTCTTTGAC AGCAATGACA CAACTTTTAA GGGGACTGTT
    GATTGCCAGA ACTGCTCCAG GCAGTTTATA GCTGAAAAGC TTATGAAGAC AAATCTGGTG
    CTTATTGTTG CTGATACAGA GATGTGCAGC CAGATGTGTG AGGAAAAACC ATTTTCCCAG
    GAAGAAAAGC CTTCTCCTGG TCCAGATCCC TGCTTGATAT CGCAGAACCC ACGGTATCGG
    AAAGGTCCAA TGGAGTGCTT TGACAACAAT GTCAATGAAA ATAATGAGCC ATGTGGAGGG
    GCAACAAGTC TGACTCCATC TCTCGTGTCC ATGGGCATCC TCCAGCTGCT GCTGCTGCTC
    TGGCTTCTGA TTGGACGAGA ACACTGCCTC TCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298562.1
    CDS266..3541
    Translation

    Target ORF information:

    RefSeq Version XM_009300287.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300287.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATC TTGGATCTTG GAAAATCTCG CAGTTTCTGA AAGCCTGCAG
    GTGGACAAGT TCGAGGAGTA TGGATATACT TTAATAGCAC CCAGAGATTA CTGTGAAGAC
    CTGAAGGCTC AGAATGAAAA CATGACACAG TTCCTCATCG ACTTCAACGA GTACATCGAC
    GTAAAGACCC CCAATGACCC ACAGTGCAAT ATGGACTTAA TGAATCGACT ACTGTTGGAT
    GCTGGCATCA CGGCTGAACT GGTCAAACAC TGGAACAAAA ATCAATTGAG TGACAGAATC
    AAGGCTAAGT TTGTGGCGAC AGATGGTGGT GTCACTAGAG TATATCCTGC AAGTGCTGGT
    TCAGAATGGA CCGGACAGGC CGAAACATAT GAGTCAAGTT TCTACAAGAG AAGCTTGGAC
    AATGATCTCT ATGTATTTAC ACCACTGCCA TTCACAGGAA ATAAAACCGA AGAGTCAGTT
    TTAGTGAGCA GAGCAATGGA CTTGAGGATC GATGAATTCT GGCTAAAACC AGCAGTTGTC
    GGTGTGAAAC TGGATGTTCA ATCCTGGATG GAAAGCTTCA TGAATACTAC AATTACGCCC
    AAATGTGAAG GTGAAATATG TGGATGCAGG AGGAACGATG AGTATCTGGA CTGTGTTCTT
    CTAGACGATG GAGGATTCCT TGTCATGGCC AATCAGGACA AATACATTGA CCAGATTGGA
    GAGTTTTTCG GCATGATTGA CCCCAGCCTA ATGAGAAATC TGATGAACGT GTCCCTGTTT
    TCAGTCAACA TAACCTACGA CTACCAGGCC CTTTGCGAAC CCACCAGAGA ATCTAAAGCT
    GCCGCTGGTC TCAGATCAGT CTATGTGCCC ACCATAGCTG ATATCCTGAA CATTGGTTGG
    TGGGCTTCTG CAGCAGCCTG GTCGATAATG CAGCAGTTGT TTGTCAGCAT TACATTTCCT
    AATTTCCTTG AGGCAGTGGA CATAAGTGAT GAGTCTGAAA CACCATCTAA AGAGAGTTGC
    ATTAAAGAGC AGACGCAGTA TTTCTTTGAC AGCAATGACA CAACTTTTAA GGGGACTGTT
    GATTGCCAGA ACTGCTCCAG GCAGTTTATA GCTGAAAAGC TTATGAAGAC AAATCTGGTG
    CTTATTGTTG CTGATACAGA GATGTGCAGC CAGATGTGTG AGGAAAAACC ATTTTCCCAG
    GAAGAAAAGC CTTCTCCTGG TCCAGATCCC TGCTTGATAT CGCAGAACCC ACGGTATCGG
    AAAGGTCCAA TGGAGTGCTT TGACAACAAT GTCAATGAAA ATAATGAGCC ATGTGGAGGG
    GCAACAAGTC TGACTCCATC TCTCGTGTCC ATGGGCATCC TCCAGCTGCT GCTGCTGCTC
    TGGCTTCTGA TTGGACGAGA ACACTGCCTC TCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43510
    Clone ID Related Accession (Same CDS sequence) XM_009300288.3 , XM_009300288.2
    Accession Version XM_009300288.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300288.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATT TTCTGAAAGC CTGCAGGTGG ACAAGTTCGA GGAGTATGGA
    TATACTTTAA TAGCACCCAG AGATTACTGT GAAGACCTGA AGGCTCAGAA TGAAAACATG
    ACACAGTTCC TCATCGACTT CAACGAGTAC ATCGACGTAA AGACCCCCAA TGACCCACAG
    TGCAATATGG ACTTAATGAA TCGACTACTG TTGGATGCTG GCATCACGGC TGAACTGGTC
    AAACACTGGA ACAAAAATCA ATTGAGTGAC AGAATCAAGG CTAAGTTTGT GGCGACAGAT
    GGTGGTGTCA CTAGAGTATA TCCTGCAAGT GCTGGTTCAG AATGGACCGG ACAGGCCGAA
    ACATATGAGT CAAGTTTCTA CAAGAGAAGC TTGGACAATG ATCTCTATGT ATTTACACCA
    CTGCCATTCA CAGGAAATAA AACCGAAGAG TCAGTTTTAG TGAGCAGAGC AATGGACTTG
    AGGATCGATG AATTCTGGCT AAAACCAGCA GTTGTCGGTG TGAAACTGGA TGTTCAATCC
    TGGATGGAAA GCTTCATGAA TACTACAATT ACGCCCAAAT GTGAAGGTGA AATATGTGGA
    TGCAGGAGGA ACGATGAGTA TCTGGACTGT GTTCTTCTAG ACGATGGAGG ATTCCTTGTC
    ATGGCCAATC AGGACAAATA CATTGACCAG ATTGGAGAGT TTTTCGGCAT GATTGACCCC
    AGCCTAATGA GAAATCTGAT GAACGTGTCC CTGTTTTCAG TCAACATAAC CTACGACTAC
    CAGGCCCTTT GCGAACCCAC CAGAGAATCT AAAGCTGCCG CTGGTCTCAG ATCAGTCTAT
    GTGCCCACCA TAGCTGATAT CCTGAACATT GGTTGGTGGG CTTCTGCAGC AGCCTGGTCG
    ATAATGCAGC AGTTGTTTGT CAGCATTACA TTTCCTAATT TCCTTGAGGC AGTGGACATA
    AGTGATGAGT CTGAAACACC ATCTAAAGAG AGTTGCATTA AAGAGCAGAC GCAGTATTTC
    TTTGACAGCA ATGACACAAC TTTTAAGGGG ACTGTTGATT GCCAGAACTG CTCCAGGCAG
    TTTATAGCTG AAAAGCTTAT GAAGACAAAT CTGGTGCTTA TTGTTGCTGA TACAGAGATG
    TGCAGCCAGA TGTGTGAGGA AAAACCATTT TCCCAGGAAG AAAAGCCTTC TCCTGGTCCA
    GATCCCTGCT TGATATCGCA GAACCCACGG TATCGGAAAG GTCCAATGGA GTGCTTTGAC
    AACAATGTCA ATGATGAGGC CATGTGTAGG AGAAATGCAG GCTCTCCACT GCTCTCCTCT
    CTCCTCATGT TACTGCTGCC AGTGTTCATG TGCTGTCTCG GGAAAATAAT GAGCCATGTG
    GAGGGGCAAC AAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298563.1
    CDS268..3525
    Translation

    Target ORF information:

    RefSeq Version XM_009300288.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300288.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATT TTCTGAAAGC CTGCAGGTGG ACAAGTTCGA GGAGTATGGA
    TATACTTTAA TAGCACCCAG AGATTACTGT GAAGACCTGA AGGCTCAGAA TGAAAACATG
    ACACAGTTCC TCATCGACTT CAACGAGTAC ATCGACGTAA AGACCCCCAA TGACCCACAG
    TGCAATATGG ACTTAATGAA TCGACTACTG TTGGATGCTG GCATCACGGC TGAACTGGTC
    AAACACTGGA ACAAAAATCA ATTGAGTGAC AGAATCAAGG CTAAGTTTGT GGCGACAGAT
    GGTGGTGTCA CTAGAGTATA TCCTGCAAGT GCTGGTTCAG AATGGACCGG ACAGGCCGAA
    ACATATGAGT CAAGTTTCTA CAAGAGAAGC TTGGACAATG ATCTCTATGT ATTTACACCA
    CTGCCATTCA CAGGAAATAA AACCGAAGAG TCAGTTTTAG TGAGCAGAGC AATGGACTTG
    AGGATCGATG AATTCTGGCT AAAACCAGCA GTTGTCGGTG TGAAACTGGA TGTTCAATCC
    TGGATGGAAA GCTTCATGAA TACTACAATT ACGCCCAAAT GTGAAGGTGA AATATGTGGA
    TGCAGGAGGA ACGATGAGTA TCTGGACTGT GTTCTTCTAG ACGATGGAGG ATTCCTTGTC
    ATGGCCAATC AGGACAAATA CATTGACCAG ATTGGAGAGT TTTTCGGCAT GATTGACCCC
    AGCCTAATGA GAAATCTGAT GAACGTGTCC CTGTTTTCAG TCAACATAAC CTACGACTAC
    CAGGCCCTTT GCGAACCCAC CAGAGAATCT AAAGCTGCCG CTGGTCTCAG ATCAGTCTAT
    GTGCCCACCA TAGCTGATAT CCTGAACATT GGTTGGTGGG CTTCTGCAGC AGCCTGGTCG
    ATAATGCAGC AGTTGTTTGT CAGCATTACA TTTCCTAATT TCCTTGAGGC AGTGGACATA
    AGTGATGAGT CTGAAACACC ATCTAAAGAG AGTTGCATTA AAGAGCAGAC GCAGTATTTC
    TTTGACAGCA ATGACACAAC TTTTAAGGGG ACTGTTGATT GCCAGAACTG CTCCAGGCAG
    TTTATAGCTG AAAAGCTTAT GAAGACAAAT CTGGTGCTTA TTGTTGCTGA TACAGAGATG
    TGCAGCCAGA TGTGTGAGGA AAAACCATTT TCCCAGGAAG AAAAGCCTTC TCCTGGTCCA
    GATCCCTGCT TGATATCGCA GAACCCACGG TATCGGAAAG GTCCAATGGA GTGCTTTGAC
    AACAATGTCA ATGATGAGGC CATGTGTAGG AGAAATGCAG GCTCTCCACT GCTCTCCTCT
    CTCCTCATGT TACTGCTGCC AGTGTTCATG TGCTGTCTCG GGAAAATAAT GAGCCATGTG
    GAGGGGCAAC AAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43510
    Clone ID Related Accession (Same CDS sequence) XM_009300288.3 , XM_009300288.2
    Accession Version XM_009300288.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATT TTCTGAAAGC CTGCAGGTGG ACAAGTTCGA GGAGTATGGA
    TATACTTTAA TAGCACCCAG AGATTACTGT GAAGACCTGA AGGCTCAGAA TGAAAACATG
    ACACAGTTCC TCATCGACTT CAACGAGTAC ATCGACGTAA AGACCCCCAA TGACCCACAG
    TGCAATATGG ACTTAATGAA TCGACTACTG TTGGATGCTG GCATCACGGC TGAACTGGTC
    AAACACTGGA ACAAAAATCA ATTGAGTGAC AGAATCAAGG CTAAGTTTGT GGCGACAGAT
    GGTGGTGTCA CTAGAGTATA TCCTGCAAGT GCTGGTTCAG AATGGACCGG ACAGGCCGAA
    ACATATGAGT CAAGTTTCTA CAAGAGAAGC TTGGACAATG ATCTCTATGT ATTTACACCA
    CTGCCATTCA CAGGAAATAA AACCGAAGAG TCAGTTTTAG TGAGCAGAGC AATGGACTTG
    AGGATCGATG AATTCTGGCT AAAACCAGCA GTTGTCGGTG TGAAACTGGA TGTTCAATCC
    TGGATGGAAA GCTTCATGAA TACTACAATT ACGCCCAAAT GTGAAGGTGA AATATGTGGA
    TGCAGGAGGA ACGATGAGTA TCTGGACTGT GTTCTTCTAG ACGATGGAGG ATTCCTTGTC
    ATGGCCAATC AGGACAAATA CATTGACCAG ATTGGAGAGT TTTTCGGCAT GATTGACCCC
    AGCCTAATGA GAAATCTGAT GAACGTGTCC CTGTTTTCAG TCAACATAAC CTACGACTAC
    CAGGCCCTTT GCGAACCCAC CAGAGAATCT AAAGCTGCCG CTGGTCTCAG ATCAGTCTAT
    GTGCCCACCA TAGCTGATAT CCTGAACATT GGTTGGTGGG CTTCTGCAGC AGCCTGGTCG
    ATAATGCAGC AGTTGTTTGT CAGCATTACA TTTCCTAATT TCCTTGAGGC AGTGGACATA
    AGTGATGAGT CTGAAACACC ATCTAAAGAG AGTTGCATTA AAGAGCAGAC GCAGTATTTC
    TTTGACAGCA ATGACACAAC TTTTAAGGGG ACTGTTGATT GCCAGAACTG CTCCAGGCAG
    TTTATAGCTG AAAAGCTTAT GAAGACAAAT CTGGTGCTTA TTGTTGCTGA TACAGAGATG
    TGCAGCCAGA TGTGTGAGGA AAAACCATTT TCCCAGGAAG AAAAGCCTTC TCCTGGTCCA
    GATCCCTGCT TGATATCGCA GAACCCACGG TATCGGAAAG GTCCAATGGA GTGCTTTGAC
    AACAATGTCA ATGATGAGGC CATGTGTAGG AGAAATGCAG GCTCTCCACT GCTCTCCTCT
    CTCCTCATGT TACTGCTGCC AGTGTTCATG TGCTGTCTCG GGAAAATAAT GAGCCATGTG
    GAGGGGCAAC AAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298563.1
    CDS277..3534
    Translation

    Target ORF information:

    RefSeq Version XM_009300288.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 1a (cacna2d1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300288.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCAGTCA GAAGAATGAT GGATGCGCTC AGATTGTCGA CCGCTCTTCT TCTACTTCTG 
    TCTTTATGCA GCTTTGGAAG CGCTATACAG TTTCCCGACT CATTTCTGAC CCGGAAATGG
    GCAGAAAAGA TGCAGGAAGA TCTCGTGCAG TTGATCGATT CTGAGAGCGG TATAAAAGAG
    CTCAAAAAAA TTTTCAATAA TTTTCAGAGA CATTATACTG TGAGGCAAAA CAATCCTCGA
    CAACTGGTGG AAAAAGCATC ACATGAAATT GAAGAATTTC TTGCCAATCG CTCCAAAGCT
    TTAGAAGCGC TGGCGACTGC TGCCGAGAAC CTGCAGAAGG AGCACCAGTG GAAAGAAGAT
    CTTGACGTGA ATGAGACCAT CTACTACAAT GCCAAGGACA AGAGTGATAA TCAGACAGAA
    CCAAGAAAGA ACCGGCTCAT ACTGGATTTC ACAGAAGATC CTGCTTTCAA GCGGCCCGTT
    TCATACAACA CCACAGCAGT GCACATTCCC ACTGATATCT ATGAGGGCTC CACTATCATC
    CTAAATGAGC TGAACTGGAC GGCGGCTCTG GATGACGTCT TCAGGAAGAA CAAAGAGGAC
    GATCCATCGC TTCACTGGCA GGTGTTCGGC AGTGCAACAG GCCTGGCACG ATATTTCCCA
    GCTTCCCCTT GGGTCGACTC TAAAAACAAG ATTGACCTGT ATGATGTACG CCGCAGGCCC
    TGGTATATTC AGGGTGCAGC TTCGCCTAAG GACATGCTGA TCTTGGTGGA CGCGAGTGGA
    AGTGTGTCAG GCCTGACGCT GAAGCTCATT CGCACCTCAG TCAGCGAGAT GCTGGAGACT
    CTCTCAGATG ACGATTACGT CAACATCGTC TCTTTTAACA ATTCAGCCAA GTCGGTGGCC
    TGTTTTGAAA ACCTGGTGCA AGCGAACGTC CGCAATAAGA AGACTTTGAA GGAGGCCGTT
    CAGAAGATCA CTGCCAACGG CACCACTGAC TATAAAATCG GCTTCAAGGA GGCTTTCAAC
    CAGCTGGCTT CTATGAATGT TTCAAGAGCA AACTGTAATA AAATCATCAT GTTATTCACT
    GATGGAGGGG AGGACAAAGC GTCAGAGATC TTTGATGAGT ACAACTCTGA CAAGAGGGTT
    AGAATCTTCA CCTTTTCTGT GGGCCAACAT AACTATGACA AGGCACCAAT ACAATACATG
    GCTTGCCATA ACAAAGGTTA TTATTATGAG ATCCCATCGA TTGGAGCCAT CAGGATCAAC
    ACGCAGGAGT ACCTGGATGT TTTAGGCAGA CCTATGGTGA AAGCAGACAA GAAAGCTAAA
    AATGTGCAAT GGACCAACGT TTATGTGGAT GCTCTGGAAC TGGGCCTGGT GGTTACAGGA
    ACTCTTCCAG TGTTCAATAA GACCAGGACC AGTAAAGAAA AGCCTAAGGG TAAGAACCTG
    GGTCCGAGTC AGATGATTTT GGGAGTGATG GCCATAGACG TGTCTCTGGA CGACATCAAG
    AGACTGACTC CACATTACAC TCTTGGGCCA AATGGATATT ACTTCGCCAT TGATCCCAAC
    GGCTACGTTC TACTTCATCC CAACCTTCAA CCCAAGGATC TCAAGTCTCA GGAACCTGTG
    ACGCTGGACT TCTTGGATGC TGAGATGGAG GATGAAGTCA AAGTGGATAT CAGAAGAAGC
    ATGATAGATG GTGAAAGAAG TCAAATACGC TTCCACACAC TTATGAAGTC ACAGGATGAG
    CGTTACATAG ACAAGGGCTT CAGGACGTAC ACTTGGTCTC CAGTCACTGA CGCAGATTAC
    AGCCTTGCGT TAGTTCTGCC AGATTACAGC ATGAGCTACA TCAAGGCCAC TATAGGAGAC
    ACCATCACTC AGGCCAAATT TTCTGAAAGC CTGCAGGTGG ACAAGTTCGA GGAGTATGGA
    TATACTTTAA TAGCACCCAG AGATTACTGT GAAGACCTGA AGGCTCAGAA TGAAAACATG
    ACACAGTTCC TCATCGACTT CAACGAGTAC ATCGACGTAA AGACCCCCAA TGACCCACAG
    TGCAATATGG ACTTAATGAA TCGACTACTG TTGGATGCTG GCATCACGGC TGAACTGGTC
    AAACACTGGA ACAAAAATCA ATTGAGTGAC AGAATCAAGG CTAAGTTTGT GGCGACAGAT
    GGTGGTGTCA CTAGAGTATA TCCTGCAAGT GCTGGTTCAG AATGGACCGG ACAGGCCGAA
    ACATATGAGT CAAGTTTCTA CAAGAGAAGC TTGGACAATG ATCTCTATGT ATTTACACCA
    CTGCCATTCA CAGGAAATAA AACCGAAGAG TCAGTTTTAG TGAGCAGAGC AATGGACTTG
    AGGATCGATG AATTCTGGCT AAAACCAGCA GTTGTCGGTG TGAAACTGGA TGTTCAATCC
    TGGATGGAAA GCTTCATGAA TACTACAATT ACGCCCAAAT GTGAAGGTGA AATATGTGGA
    TGCAGGAGGA ACGATGAGTA TCTGGACTGT GTTCTTCTAG ACGATGGAGG ATTCCTTGTC
    ATGGCCAATC AGGACAAATA CATTGACCAG ATTGGAGAGT TTTTCGGCAT GATTGACCCC
    AGCCTAATGA GAAATCTGAT GAACGTGTCC CTGTTTTCAG TCAACATAAC CTACGACTAC
    CAGGCCCTTT GCGAACCCAC CAGAGAATCT AAAGCTGCCG CTGGTCTCAG ATCAGTCTAT
    GTGCCCACCA TAGCTGATAT CCTGAACATT GGTTGGTGGG CTTCTGCAGC AGCCTGGTCG
    ATAATGCAGC AGTTGTTTGT CAGCATTACA TTTCCTAATT TCCTTGAGGC AGTGGACATA
    AGTGATGAGT CTGAAACACC ATCTAAAGAG AGTTGCATTA AAGAGCAGAC GCAGTATTTC
    TTTGACAGCA ATGACACAAC TTTTAAGGGG ACTGTTGATT GCCAGAACTG CTCCAGGCAG
    TTTATAGCTG AAAAGCTTAT GAAGACAAAT CTGGTGCTTA TTGTTGCTGA TACAGAGATG
    TGCAGCCAGA TGTGTGAGGA AAAACCATTT TCCCAGGAAG AAAAGCCTTC TCCTGGTCCA
    GATCCCTGCT TGATATCGCA GAACCCACGG TATCGGAAAG GTCCAATGGA GTGCTTTGAC
    AACAATGTCA ATGATGAGGC CATGTGTAGG AGAAATGCAG GCTCTCCACT GCTCTCCTCT
    CTCCTCATGT TACTGCTGCC AGTGTTCATG TGCTGTCTCG GGAAAATAAT GAGCCATGTG
    GAGGGGCAAC AAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.