GUCY1B1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY1B1
Entrez Gene ID	282433
Full Name	guanylate cyclase 1 soluble subunit beta 1
Synonyms	GUCY1B3
General protein information	Preferred Names guanylate cyclase 1 soluble subunit beta 1 Names guanylate cyclase soluble subunit beta-1 GCS-beta-1 GCS-beta-3 NO-sensitive GC beta 1 subunit guanylate cyclase 1, soluble, beta 3 guanylate cyclase soluble subunit beta-3 nitric oxide-sensitive guanylyl cyclase beta 1 subunit soluble guanylate cyclase small subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024977027.1	XP_024832795.1	guanylate cyclase soluble subunit beta-1 isoform X2
XM_010813816.2	XP_010812118.1	guanylate cyclase soluble subunit beta-1 isoform X1
XM_010813816.3	XP_010812118.1	guanylate cyclase soluble subunit beta-1 isoform X1
NM_174641.1	NP_777066.1	guanylate cyclase soluble subunit beta-1
XM_024977025.1	XP_024832793.1	guanylate cyclase soluble subunit beta-1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GUCY1B3	NP_000848.1
	GUCY1B3	XP_002804282.1
Canis lupus familiaris (dog)	GUCY1B3	NP_001018044.1
Danio rerio (zebrafish)	gucy1b3	NP_001238874.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000214	XP_310920.4
Caenorhabditis elegans (roundworm)	gcy-36	NP_510557.3
Caenorhabditis elegans (roundworm)	gcy-37	NP_500171.2
Pan troglodytes (chimpanzee)	GUCY1B3	XP_003310602.1
Mus musculus (house mouse)	Gucy1b3	NP_059497.1
Rattus norvegicus (Norway rat)	Gucy1b3	NP_036901.2
Gallus gallus (chicken)	GUCY1B3	XP_420376.3
Drosophila melanogaster (fruit fly)	Gycbeta100B	NP_524603.2
Bos taurus (cattle)	GUCY1B3	NP_777066.1
Caenorhabditis elegans (roundworm)	gcy-32	NP_506452.5
Caenorhabditis elegans (roundworm)	gcy-35	NP_001252131.1
Caenorhabditis elegans (roundworm)	gcy-34	NP_506319.1
Xenopus tropicalis (tropical clawed frog)	gucy1b3	XP_002933520.1

Gene Ontology
Bibliography

Related articles in PubMed

Heat Shock Protein 90 Associates with the Per-Arnt-Sim Domain of Heme-free Soluble Guanylate Cyclase: IMplications for Enzyme Maturation.
Sarkar A, Dai Y, Haque MM, Seeger F, Ghosh A, Garcin ED, Montfort WR, Hazen SL, Misra S, Stuehr DJ
The Journal of biological chemistry290(35)21615-28(2015 Aug)

Quaternary structure controls ligand dynamics in soluble guanylate cyclase.
Yoo BK, Lamarre I, Martin JL, Negrerie M
The Journal of biological chemistry287(9)6851-9(2012 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Dimerization of nitric oxide-sensitive guanylyl cyclase requires the alpha 1 N terminus.
Wagner C, Russwurm M, J?ger R, Friebe A, Koesling D
The Journal of biological chemistry280(18)17687-93(2005 May)

Identification of histidine 105 in the beta1 subunit of soluble guanylate cyclase as the heme proximal ligand.
Zhao Y, Schelvis JP, Babcock GT, Marletta MA
Biochemistry37(13)4502-9(1998 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest a means by which the hsp90beta interaction could prevent apo-sGCbeta1 from associating with its partner sGCalpha1 subunit while enabling structural changes to assist heme insertion into the H-NOX domain.
Title: Heat Shock Protein 90 Associates with the Per-Arnt-Sim Domain of Heme-free Soluble Guanylate Cyclase: IMplications for Enzyme Maturation.

We concluded that the alpha-subunit and the beta(1)(191-619) domain exert structural strains on the heme domain.
Title: Quaternary structure controls ligand dynamics in soluble guanylate cyclase.

The following GUCY1B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY1B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41626	NM_174641.1 Latest version!	Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa45440	XM_024977025.1 Latest version!	Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa45440	XM_010813816.3 Latest version!	Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa224157	XM_024977027.1 Latest version!	Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa45440	XM_010813816.2	Bos taurus guanylate cyclase 1, soluble, beta 3 (GUCY1B3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY1B1 ( NM_174641.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777066.1 Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), mRNA.

Price & Availability↑

CloneID	OBa41626
Clone ID Related Accession (Same CDS sequence)	NM_174641.1
Accession Version	NM_174641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	guanylate cyclase soluble subunit beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y00770.1. ##Evidence-Data-START## Transcript exon combination :: Y00770.1, BC133308.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTATGGAT TCGTGAATCA CGCGCTGGAG CTGCTGGTGA TCCGCAACTA CGGCCCTGAG 
GTGTGGGAAG ACATCAAAAA AGAGGCACAA TTAGATGAAG AAGGACAGTT TCTTGTCCGA 
ATAATTTATG ATGATTCTAA AACATATGAT TTGGTTGCTG CTGCAAGCAA GGTCCTCAAT 
CTCAACGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TCTTCGTCTT CTGTCAAGAA 
TCTGGTTATG ATACAATCCT GCGCGTCCTG GGATCTAACG TCAGAGAATT TCTGCAGAAC 
CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GCATGCGGGC ACCTTCCTTC 
CGGTGCACTG ATGCGGACAA GGGCAAAGGA CTCATCCTGC ACTACTACTC AGAGAGAGAA 
GGGCTGCAGG ATATTGTCAT CGGAATCATC AAGACAGTAG CTCAGCAGAT ACATGGCACT 
GAAATTGACA TGAAGGTTAT TCAACAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTG 
ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA 
AACGGTACCC AGGAATCACG CATCAGCCCA TATACGTTCT GCAAAGCCTT TCCCTTTCAC 
ATCATATTTG ACAGGGATCT TGTGGTCACT CAGTGTGGCA ACGCCATCTA CAGAGTCCTT 
CCCCAGCTCC AGCCTGGGAA CTGCAGCCTC CTGTCTGTCT TCTCCCTGGT CCGTCCTCAT 
ATTGACATCA GTTTCCATGG AATCCTCTCA CACATCAATA CAGTCTTCGT GTTGAGAAGC 
AAGGAAGGGT TGTTGGATGT AGAGAAATCA GAGTGTGAGG ATGAGCTGAC GGGCACTGAA 
ATCAGCTGCT TACGGCTCAA GGGTCAGATG ATCTACTTGC CCGAAGCAGA CAGCATACTT 
TTCCTGTGCT CACCAAGTGT CATGAACCTG GACGACCTGA CCCGGCGAGG GCTGTACCTG 
AGTGACATCC CTCTGCACGA TGCCACTCGG GACCTCGTGC TCCTGGGAGA GCAGTTCAGA 
GAGGAGTACA AGCTGACCCA GGAACTGGAA ATCCTCACAG ACAGGCTGCA GCTCACGTTA 
CGAGCCCTGG AAGATGAAAA GAAAAAGACG GACACGTTGC TGTATTCTGT GCTTCCTCCA 
TCTGTGGCCA ACGAGCTGAG GCACAAGCGT CCGGTGCCTG CGAAGAGATA TGACAACGTG 
ACTATCCTCT TCAGCGGCAT TGTGGGCTTC AATGCTTTCT GCAGCAAGCA CGCGTCTGGG 
GAAGGGGCCA TGAAGATTGT CAACCTCCTC AATGACCTCT ACACTAGATT TGACACTCTG 
ACGGACTCCC GGAAAAATCC ATTTGTTTAT AAGGTGGAGA CTGTCGGGGA CAAGTACATG 
ACAGTGAGCG GGCTGCCGGA GCCATGCATC CACCACGCAC GGTCCATCTG CCACCTGGCC 
TTGGATATGA TGGAGATTGC TGGCCAGGTC CAAGTAGATG GTGAATCTGT TCAGATAACG 
ATCGGGATCC ACACAGGGGA GGTAGTGACC GGAGTCATAG GACAGAGGAT GCCTCGCTAC 
TGTCTTTTTG GAAACACTGT CAACCTCACA AGCCGAACAG AAACCACTGG AGAAAAAGGG 
AAAATAAATG TGTCTGAATA TACATACAGA TGTCTCATGA CACCAGAAAA TTCAGACCCG 
CAATTCCATT TGGAGCACAG AGGCCCCGTG TCCATGAAGG GCAAAAAGGA GCCAATGCAA 
GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CGGAGCAGGA TGAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777066.1
CDS	87..1946
Translation	MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDADKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMTPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETEQDEN

Target ORF information:

RefSeq Version	NM_174641.1
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174641.1

ORF Insert Sequence:

ATGTATGGAT TCGTGAATCA CGCGCTGGAG CTGCTGGTGA TCCGCAACTA CGGCCCTGAG 
GTGTGGGAAG ACATCAAAAA AGAGGCACAA TTAGATGAAG AAGGACAGTT TCTTGTCCGA 
ATAATTTATG ATGATTCTAA AACATATGAT TTGGTTGCTG CTGCAAGCAA GGTCCTCAAT 
CTCAACGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TCTTCGTCTT CTGTCAAGAA 
TCTGGTTATG ATACAATCCT GCGCGTCCTG GGATCTAACG TCAGAGAATT TCTGCAGAAC 
CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GCATGCGGGC ACCTTCCTTC 
CGGTGCACTG ATGCGGACAA GGGCAAAGGA CTCATCCTGC ACTACTACTC AGAGAGAGAA 
GGGCTGCAGG ATATTGTCAT CGGAATCATC AAGACAGTAG CTCAGCAGAT ACATGGCACT 
GAAATTGACA TGAAGGTTAT TCAACAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTG 
ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA 
AACGGTACCC AGGAATCACG CATCAGCCCA TATACGTTCT GCAAAGCCTT TCCCTTTCAC 
ATCATATTTG ACAGGGATCT TGTGGTCACT CAGTGTGGCA ACGCCATCTA CAGAGTCCTT 
CCCCAGCTCC AGCCTGGGAA CTGCAGCCTC CTGTCTGTCT TCTCCCTGGT CCGTCCTCAT 
ATTGACATCA GTTTCCATGG AATCCTCTCA CACATCAATA CAGTCTTCGT GTTGAGAAGC 
AAGGAAGGGT TGTTGGATGT AGAGAAATCA GAGTGTGAGG ATGAGCTGAC GGGCACTGAA 
ATCAGCTGCT TACGGCTCAA GGGTCAGATG ATCTACTTGC CCGAAGCAGA CAGCATACTT 
TTCCTGTGCT CACCAAGTGT CATGAACCTG GACGACCTGA CCCGGCGAGG GCTGTACCTG 
AGTGACATCC CTCTGCACGA TGCCACTCGG GACCTCGTGC TCCTGGGAGA GCAGTTCAGA 
GAGGAGTACA AGCTGACCCA GGAACTGGAA ATCCTCACAG ACAGGCTGCA GCTCACGTTA 
CGAGCCCTGG AAGATGAAAA GAAAAAGACG GACACGTTGC TGTATTCTGT GCTTCCTCCA 
TCTGTGGCCA ACGAGCTGAG GCACAAGCGT CCGGTGCCTG CGAAGAGATA TGACAACGTG 
ACTATCCTCT TCAGCGGCAT TGTGGGCTTC AATGCTTTCT GCAGCAAGCA CGCGTCTGGG 
GAAGGGGCCA TGAAGATTGT CAACCTCCTC AATGACCTCT ACACTAGATT TGACACTCTG 
ACGGACTCCC GGAAAAATCC ATTTGTTTAT AAGGTGGAGA CTGTCGGGGA CAAGTACATG 
ACAGTGAGCG GGCTGCCGGA GCCATGCATC CACCACGCAC GGTCCATCTG CCACCTGGCC 
TTGGATATGA TGGAGATTGC TGGCCAGGTC CAAGTAGATG GTGAATCTGT TCAGATAACG 
ATCGGGATCC ACACAGGGGA GGTAGTGACC GGAGTCATAG GACAGAGGAT GCCTCGCTAC 
TGTCTTTTTG GAAACACTGT CAACCTCACA AGCCGAACAG AAACCACTGG AGAAAAAGGG 
AAAATAAATG TGTCTGAATA TACATACAGA TGTCTCATGA CACCAGAAAA TTCAGACCCG 
CAATTCCATT TGGAGCACAG AGGCCCCGTG TCCATGAAGG GCAAAAAGGA GCCAATGCAA 
GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CGGAGCAGGA TGAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1B1 ( XM_010813816.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812118.1 Bos taurus guanylate cyclase 1, soluble, beta 3 (GUCY1B3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45440
Clone ID Related Accession (Same CDS sequence)	XM_010813816.2 , XM_010813816.3 , XM_024977025.1
Accession Version	XM_010813816.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanylate cyclase soluble subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104452.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGA AGATGTTCTT CGTCTTCTGT CAAGAATCTG GTTATGATAC AATCCTGCGC 
GTCCTGGGAT CTAACGTCAG AGAATTTCTG CAGAACCTTG ATGCTCTGCA CGACCACCTT 
GCTACCATCT ACCCAGGCAT GCGGGCACCT TCCTTCCGGT GCACTGATGC GGACAAGGGC 
AAAGGACTCA TCCTGCACTA CTACTCAGAG AGAGAAGGGC TGCAGGATAT TGTCATCGGA 
ATCATCAAGA CAGTAGCTCA GCAGATACAT GGCACTGAAA TTGACATGAA GGTTATTCAA 
CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTGATTG AAGAAAAAGA GTCAAAAGAA 
GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAACG GTACCCAGGA ATCACGCATC 
AGCCCATATA CGTTCTGCAA AGCCTTTCCC TTTCACATCA TATTTGACAG GGATCTTGTG 
GTCACTCAGT GTGGCAACGC CATCTACAGA GTCCTTCCCC AGCTCCAGCC TGGGAACTGC 
AGCCTCCTGT CTGTCTTCTC CCTGGTCCGT CCTCATATTG ACATCAGTTT CCATGGAATC 
CTCTCACACA TCAATACAGT CTTCGTGTTG AGAAGCAAGG AAGGGTTGTT GGATGTAGAG 
AAATCAGAGT GTGAGGATGA GCTGACGGGC ACTGAAATCA GCTGCTTACG GCTCAAGGGT 
CAGATGATCT ACTTGCCCGA AGCAGACAGC ATACTTTTCC TGTGCTCACC AAGTGTCATG 
AACCTGGACG ACCTGACCCG GCGAGGGCTG TACCTGAGTG ACATCCCTCT GCACGATGCC 
ACTCGGGACC TCGTGCTCCT GGGAGAGCAG TTCAGAGAGG AGTACAAGCT GACCCAGGAA 
CTGGAAATCC TCACAGACAG GCTGCAGCTC ACGTTACGAG CCCTGGAAGA TGAAAAGAAA 
AAGACGGACA CGTTGCTGTA TTCTGTGCTT CCTCCATCTG TGGCCAACGA GCTGAGGCAC 
AAGCGTCCGG TGCCTGCGAA GAGATATGAC AACGTGACTA TCCTCTTCAG CGGCATTGTG 
GGCTTCAATG CTTTCTGCAG CAAGCACGCG TCTGGGGAAG GGGCCATGAA GATTGTCAAC 
CTCCTCAATG ACCTCTACAC TAGATTTGAC ACTCTGACGG ACTCCCGGAA AAATCCATTT 
GTTTATAAGG TGGAGACTGT CGGGGACAAG TACATGACAG TGAGCGGGCT GCCGGAGCCA 
TGCATCCACC ACGCACGGTC CATCTGCCAC CTGGCCTTGG ATATGATGGA GATTGCTGGC 
CAGGTCCAAG TAGATGGTGA ATCTGTTCAG ATAACGATCG GGATCCACAC AGGGGAGGTA 
GTGACCGGAG TCATAGGACA GAGGATGCCT CGCTACTGTC TTTTTGGAAA CACTGTCAAC 
CTCACAAGCC GAACAGAAAC CACTGGAGAA AAAGGGAAAA TAAATGTGTC TGAATATACA 
TACAGATGTC TCATGACACC AGAAAATTCA GACCCGCAAT TCCATTTGGA GCACAGAGGC 
CCCGTGTCCA TGAAGGGCAA AAAGGAGCCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT 
ACAGGAACAG AGGAAACGGA GCAGGATGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812118.1
CDS	285..1940
Translation	MFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDADKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMTPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETEQDEN

Target ORF information:

RefSeq Version	XM_010813816.2
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 1, soluble, beta 3 (GUCY1B3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813816.2

ORF Insert Sequence:

ATGTTTGGGA AGATGTTCTT CGTCTTCTGT CAAGAATCTG GTTATGATAC AATCCTGCGC 
GTCCTGGGAT CTAACGTCAG AGAATTTCTG CAGAACCTTG ATGCTCTGCA CGACCACCTT 
GCTACCATCT ACCCAGGCAT GCGGGCACCT TCCTTCCGGT GCACTGATGC GGACAAGGGC 
AAAGGACTCA TCCTGCACTA CTACTCAGAG AGAGAAGGGC TGCAGGATAT TGTCATCGGA 
ATCATCAAGA CAGTAGCTCA GCAGATACAT GGCACTGAAA TTGACATGAA GGTTATTCAA 
CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTGATTG AAGAAAAAGA GTCAAAAGAA 
GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAACG GTACCCAGGA ATCACGCATC 
AGCCCATATA CGTTCTGCAA AGCCTTTCCC TTTCACATCA TATTTGACAG GGATCTTGTG 
GTCACTCAGT GTGGCAACGC CATCTACAGA GTCCTTCCCC AGCTCCAGCC TGGGAACTGC 
AGCCTCCTGT CTGTCTTCTC CCTGGTCCGT CCTCATATTG ACATCAGTTT CCATGGAATC 
CTCTCACACA TCAATACAGT CTTCGTGTTG AGAAGCAAGG AAGGGTTGTT GGATGTAGAG 
AAATCAGAGT GTGAGGATGA GCTGACGGGC ACTGAAATCA GCTGCTTACG GCTCAAGGGT 
CAGATGATCT ACTTGCCCGA AGCAGACAGC ATACTTTTCC TGTGCTCACC AAGTGTCATG 
AACCTGGACG ACCTGACCCG GCGAGGGCTG TACCTGAGTG ACATCCCTCT GCACGATGCC 
ACTCGGGACC TCGTGCTCCT GGGAGAGCAG TTCAGAGAGG AGTACAAGCT GACCCAGGAA 
CTGGAAATCC TCACAGACAG GCTGCAGCTC ACGTTACGAG CCCTGGAAGA TGAAAAGAAA 
AAGACGGACA CGTTGCTGTA TTCTGTGCTT CCTCCATCTG TGGCCAACGA GCTGAGGCAC 
AAGCGTCCGG TGCCTGCGAA GAGATATGAC AACGTGACTA TCCTCTTCAG CGGCATTGTG 
GGCTTCAATG CTTTCTGCAG CAAGCACGCG TCTGGGGAAG GGGCCATGAA GATTGTCAAC 
CTCCTCAATG ACCTCTACAC TAGATTTGAC ACTCTGACGG ACTCCCGGAA AAATCCATTT 
GTTTATAAGG TGGAGACTGT CGGGGACAAG TACATGACAG TGAGCGGGCT GCCGGAGCCA 
TGCATCCACC ACGCACGGTC CATCTGCCAC CTGGCCTTGG ATATGATGGA GATTGCTGGC 
CAGGTCCAAG TAGATGGTGA ATCTGTTCAG ATAACGATCG GGATCCACAC AGGGGAGGTA 
GTGACCGGAG TCATAGGACA GAGGATGCCT CGCTACTGTC TTTTTGGAAA CACTGTCAAC 
CTCACAAGCC GAACAGAAAC CACTGGAGAA AAAGGGAAAA TAAATGTGTC TGAATATACA 
TACAGATGTC TCATGACACC AGAAAATTCA GACCCGCAAT TCCATTTGGA GCACAGAGGC 
CCCGTGTCCA TGAAGGGCAA AAAGGAGCCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT 
ACAGGAACAG AGGAAACGGA GCAGGATGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1B1 ( XM_010813816.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812118.1 Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45440
Clone ID Related Accession (Same CDS sequence)	XM_010813816.2 , XM_010813816.3 , XM_024977025.1
Accession Version	XM_010813816.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanylate cyclase soluble subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGA AGATGTTCTT CGTCTTCTGT CAAGAATCTG GTTATGATAC AATCCTGCGC 
GTCCTGGGAT CTAACGTCAG AGAATTTCTG CAGAACCTTG ATGCTCTGCA CGACCACCTT 
GCTACCATCT ACCCAGGCAT GCGGGCACCT TCCTTCCGGT GCACTGATGC GGACAAGGGC 
AAAGGACTCA TCCTGCACTA CTACTCAGAG AGAGAAGGGC TGCAGGATAT TGTCATCGGA 
ATCATCAAGA CAGTAGCTCA GCAGATACAT GGCACTGAAA TTGACATGAA GGTTATTCAA 
CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTGATTG AAGAAAAAGA GTCAAAAGAA 
GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAACG GTACCCAGGA ATCACGCATC 
AGCCCATATA CGTTCTGCAA AGCCTTTCCC TTTCACATCA TATTTGACAG GGATCTTGTG 
GTCACTCAGT GTGGCAACGC CATCTACAGA GTCCTTCCCC AGCTCCAGCC TGGGAACTGC 
AGCCTCCTGT CTGTCTTCTC CCTGGTCCGT CCTCATATTG ACATCAGTTT CCATGGAATC 
CTCTCACACA TCAATACAGT CTTCGTGTTG AGAAGCAAGG AAGGGTTGTT GGATGTAGAG 
AAATCAGAGT GTGAGGATGA GCTGACGGGC ACTGAAATCA GCTGCTTACG GCTCAAGGGT 
CAGATGATCT ACTTGCCCGA AGCAGACAGC ATACTTTTCC TGTGCTCACC AAGTGTCATG 
AACCTGGACG ACCTGACCCG GCGAGGGCTG TACCTGAGTG ACATCCCTCT GCACGATGCC 
ACTCGGGACC TCGTGCTCCT GGGAGAGCAG TTCAGAGAGG AGTACAAGCT GACCCAGGAA 
CTGGAAATCC TCACAGACAG GCTGCAGCTC ACGTTACGAG CCCTGGAAGA TGAAAAGAAA 
AAGACGGACA CGTTGCTGTA TTCTGTGCTT CCTCCATCTG TGGCCAACGA GCTGAGGCAC 
AAGCGTCCGG TGCCTGCGAA GAGATATGAC AACGTGACTA TCCTCTTCAG CGGCATTGTG 
GGCTTCAATG CTTTCTGCAG CAAGCACGCG TCTGGGGAAG GGGCCATGAA GATTGTCAAC 
CTCCTCAATG ACCTCTACAC TAGATTTGAC ACTCTGACGG ACTCCCGGAA AAATCCATTT 
GTTTATAAGG TGGAGACTGT CGGGGACAAG TACATGACAG TGAGCGGGCT GCCGGAGCCA 
TGCATCCACC ACGCACGGTC CATCTGCCAC CTGGCCTTGG ATATGATGGA GATTGCTGGC 
CAGGTCCAAG TAGATGGTGA ATCTGTTCAG ATAACGATCG GGATCCACAC AGGGGAGGTA 
GTGACCGGAG TCATAGGACA GAGGATGCCT CGCTACTGTC TTTTTGGAAA CACTGTCAAC 
CTCACAAGCC GAACAGAAAC CACTGGAGAA AAAGGGAAAA TAAATGTGTC TGAATATACA 
TACAGATGTC TCATGACACC AGAAAATTCA GACCCGCAAT TCCATTTGGA GCACAGAGGC 
CCCGTGTCCA TGAAGGGCAA AAAGGAGCCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT 
ACAGGAACAG AGGAAACGGA GCAGGATGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812118.1
CDS	373..2028
Translation	MFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDADKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMTPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETEQDEN

Target ORF information:

RefSeq Version	XM_010813816.3
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813816.3

ORF Insert Sequence:

ATGTTTGGGA AGATGTTCTT CGTCTTCTGT CAAGAATCTG GTTATGATAC AATCCTGCGC 
GTCCTGGGAT CTAACGTCAG AGAATTTCTG CAGAACCTTG ATGCTCTGCA CGACCACCTT 
GCTACCATCT ACCCAGGCAT GCGGGCACCT TCCTTCCGGT GCACTGATGC GGACAAGGGC 
AAAGGACTCA TCCTGCACTA CTACTCAGAG AGAGAAGGGC TGCAGGATAT TGTCATCGGA 
ATCATCAAGA CAGTAGCTCA GCAGATACAT GGCACTGAAA TTGACATGAA GGTTATTCAA 
CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTGATTG AAGAAAAAGA GTCAAAAGAA 
GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAACG GTACCCAGGA ATCACGCATC 
AGCCCATATA CGTTCTGCAA AGCCTTTCCC TTTCACATCA TATTTGACAG GGATCTTGTG 
GTCACTCAGT GTGGCAACGC CATCTACAGA GTCCTTCCCC AGCTCCAGCC TGGGAACTGC 
AGCCTCCTGT CTGTCTTCTC CCTGGTCCGT CCTCATATTG ACATCAGTTT CCATGGAATC 
CTCTCACACA TCAATACAGT CTTCGTGTTG AGAAGCAAGG AAGGGTTGTT GGATGTAGAG 
AAATCAGAGT GTGAGGATGA GCTGACGGGC ACTGAAATCA GCTGCTTACG GCTCAAGGGT 
CAGATGATCT ACTTGCCCGA AGCAGACAGC ATACTTTTCC TGTGCTCACC AAGTGTCATG 
AACCTGGACG ACCTGACCCG GCGAGGGCTG TACCTGAGTG ACATCCCTCT GCACGATGCC 
ACTCGGGACC TCGTGCTCCT GGGAGAGCAG TTCAGAGAGG AGTACAAGCT GACCCAGGAA 
CTGGAAATCC TCACAGACAG GCTGCAGCTC ACGTTACGAG CCCTGGAAGA TGAAAAGAAA 
AAGACGGACA CGTTGCTGTA TTCTGTGCTT CCTCCATCTG TGGCCAACGA GCTGAGGCAC 
AAGCGTCCGG TGCCTGCGAA GAGATATGAC AACGTGACTA TCCTCTTCAG CGGCATTGTG 
GGCTTCAATG CTTTCTGCAG CAAGCACGCG TCTGGGGAAG GGGCCATGAA GATTGTCAAC 
CTCCTCAATG ACCTCTACAC TAGATTTGAC ACTCTGACGG ACTCCCGGAA AAATCCATTT 
GTTTATAAGG TGGAGACTGT CGGGGACAAG TACATGACAG TGAGCGGGCT GCCGGAGCCA 
TGCATCCACC ACGCACGGTC CATCTGCCAC CTGGCCTTGG ATATGATGGA GATTGCTGGC 
CAGGTCCAAG TAGATGGTGA ATCTGTTCAG ATAACGATCG GGATCCACAC AGGGGAGGTA 
GTGACCGGAG TCATAGGACA GAGGATGCCT CGCTACTGTC TTTTTGGAAA CACTGTCAAC 
CTCACAAGCC GAACAGAAAC CACTGGAGAA AAAGGGAAAA TAAATGTGTC TGAATATACA 
TACAGATGTC TCATGACACC AGAAAATTCA GACCCGCAAT TCCATTTGGA GCACAGAGGC 
CCCGTGTCCA TGAAGGGCAA AAAGGAGCCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT 
ACAGGAACAG AGGAAACGGA GCAGGATGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1B1 ( XM_024977025.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832793.1 Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45440
Clone ID Related Accession (Same CDS sequence)	XM_010813816.2 , XM_010813816.3 , XM_024977025.1
Accession Version	XM_024977025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanylate cyclase soluble subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGA AGATGTTCTT CGTCTTCTGT CAAGAATCTG GTTATGATAC AATCCTGCGC 
GTCCTGGGAT CTAACGTCAG AGAATTTCTG CAGAACCTTG ATGCTCTGCA CGACCACCTT 
GCTACCATCT ACCCAGGCAT GCGGGCACCT TCCTTCCGGT GCACTGATGC GGACAAGGGC 
AAAGGACTCA TCCTGCACTA CTACTCAGAG AGAGAAGGGC TGCAGGATAT TGTCATCGGA 
ATCATCAAGA CAGTAGCTCA GCAGATACAT GGCACTGAAA TTGACATGAA GGTTATTCAA 
CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTGATTG AAGAAAAAGA GTCAAAAGAA 
GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAACG GTACCCAGGA ATCACGCATC 
AGCCCATATA CGTTCTGCAA AGCCTTTCCC TTTCACATCA TATTTGACAG GGATCTTGTG 
GTCACTCAGT GTGGCAACGC CATCTACAGA GTCCTTCCCC AGCTCCAGCC TGGGAACTGC 
AGCCTCCTGT CTGTCTTCTC CCTGGTCCGT CCTCATATTG ACATCAGTTT CCATGGAATC 
CTCTCACACA TCAATACAGT CTTCGTGTTG AGAAGCAAGG AAGGGTTGTT GGATGTAGAG 
AAATCAGAGT GTGAGGATGA GCTGACGGGC ACTGAAATCA GCTGCTTACG GCTCAAGGGT 
CAGATGATCT ACTTGCCCGA AGCAGACAGC ATACTTTTCC TGTGCTCACC AAGTGTCATG 
AACCTGGACG ACCTGACCCG GCGAGGGCTG TACCTGAGTG ACATCCCTCT GCACGATGCC 
ACTCGGGACC TCGTGCTCCT GGGAGAGCAG TTCAGAGAGG AGTACAAGCT GACCCAGGAA 
CTGGAAATCC TCACAGACAG GCTGCAGCTC ACGTTACGAG CCCTGGAAGA TGAAAAGAAA 
AAGACGGACA CGTTGCTGTA TTCTGTGCTT CCTCCATCTG TGGCCAACGA GCTGAGGCAC 
AAGCGTCCGG TGCCTGCGAA GAGATATGAC AACGTGACTA TCCTCTTCAG CGGCATTGTG 
GGCTTCAATG CTTTCTGCAG CAAGCACGCG TCTGGGGAAG GGGCCATGAA GATTGTCAAC 
CTCCTCAATG ACCTCTACAC TAGATTTGAC ACTCTGACGG ACTCCCGGAA AAATCCATTT 
GTTTATAAGG TGGAGACTGT CGGGGACAAG TACATGACAG TGAGCGGGCT GCCGGAGCCA 
TGCATCCACC ACGCACGGTC CATCTGCCAC CTGGCCTTGG ATATGATGGA GATTGCTGGC 
CAGGTCCAAG TAGATGGTGA ATCTGTTCAG ATAACGATCG GGATCCACAC AGGGGAGGTA 
GTGACCGGAG TCATAGGACA GAGGATGCCT CGCTACTGTC TTTTTGGAAA CACTGTCAAC 
CTCACAAGCC GAACAGAAAC CACTGGAGAA AAAGGGAAAA TAAATGTGTC TGAATATACA 
TACAGATGTC TCATGACACC AGAAAATTCA GACCCGCAAT TCCATTTGGA GCACAGAGGC 
CCCGTGTCCA TGAAGGGCAA AAAGGAGCCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT 
ACAGGAACAG AGGAAACGGA GCAGGATGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832793.1
CDS	212..1867
Translation	MFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDADKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMTPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETEQDEN

Target ORF information:

RefSeq Version	XM_024977025.1
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977025.1

ORF Insert Sequence:

ATGTTTGGGA AGATGTTCTT CGTCTTCTGT CAAGAATCTG GTTATGATAC AATCCTGCGC 
GTCCTGGGAT CTAACGTCAG AGAATTTCTG CAGAACCTTG ATGCTCTGCA CGACCACCTT 
GCTACCATCT ACCCAGGCAT GCGGGCACCT TCCTTCCGGT GCACTGATGC GGACAAGGGC 
AAAGGACTCA TCCTGCACTA CTACTCAGAG AGAGAAGGGC TGCAGGATAT TGTCATCGGA 
ATCATCAAGA CAGTAGCTCA GCAGATACAT GGCACTGAAA TTGACATGAA GGTTATTCAA 
CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTGATTG AAGAAAAAGA GTCAAAAGAA 
GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAACG GTACCCAGGA ATCACGCATC 
AGCCCATATA CGTTCTGCAA AGCCTTTCCC TTTCACATCA TATTTGACAG GGATCTTGTG 
GTCACTCAGT GTGGCAACGC CATCTACAGA GTCCTTCCCC AGCTCCAGCC TGGGAACTGC 
AGCCTCCTGT CTGTCTTCTC CCTGGTCCGT CCTCATATTG ACATCAGTTT CCATGGAATC 
CTCTCACACA TCAATACAGT CTTCGTGTTG AGAAGCAAGG AAGGGTTGTT GGATGTAGAG 
AAATCAGAGT GTGAGGATGA GCTGACGGGC ACTGAAATCA GCTGCTTACG GCTCAAGGGT 
CAGATGATCT ACTTGCCCGA AGCAGACAGC ATACTTTTCC TGTGCTCACC AAGTGTCATG 
AACCTGGACG ACCTGACCCG GCGAGGGCTG TACCTGAGTG ACATCCCTCT GCACGATGCC 
ACTCGGGACC TCGTGCTCCT GGGAGAGCAG TTCAGAGAGG AGTACAAGCT GACCCAGGAA 
CTGGAAATCC TCACAGACAG GCTGCAGCTC ACGTTACGAG CCCTGGAAGA TGAAAAGAAA 
AAGACGGACA CGTTGCTGTA TTCTGTGCTT CCTCCATCTG TGGCCAACGA GCTGAGGCAC 
AAGCGTCCGG TGCCTGCGAA GAGATATGAC AACGTGACTA TCCTCTTCAG CGGCATTGTG 
GGCTTCAATG CTTTCTGCAG CAAGCACGCG TCTGGGGAAG GGGCCATGAA GATTGTCAAC 
CTCCTCAATG ACCTCTACAC TAGATTTGAC ACTCTGACGG ACTCCCGGAA AAATCCATTT 
GTTTATAAGG TGGAGACTGT CGGGGACAAG TACATGACAG TGAGCGGGCT GCCGGAGCCA 
TGCATCCACC ACGCACGGTC CATCTGCCAC CTGGCCTTGG ATATGATGGA GATTGCTGGC 
CAGGTCCAAG TAGATGGTGA ATCTGTTCAG ATAACGATCG GGATCCACAC AGGGGAGGTA 
GTGACCGGAG TCATAGGACA GAGGATGCCT CGCTACTGTC TTTTTGGAAA CACTGTCAAC 
CTCACAAGCC GAACAGAAAC CACTGGAGAA AAAGGGAAAA TAAATGTGTC TGAATATACA 
TACAGATGTC TCATGACACC AGAAAATTCA GACCCGCAAT TCCATTTGGA GCACAGAGGC 
CCCGTGTCCA TGAAGGGCAA AAAGGAGCCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT 
ACAGGAACAG AGGAAACGGA GCAGGATGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1B1 ( XM_024977027.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832795.1 Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa224157
Clone ID Related Accession (Same CDS sequence)	XM_024977027.1
Accession Version	XM_024977027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanylate cyclase soluble subunit beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCAC CTTCCTTCCG GTGCACTGAT GCGGACAAGG GCAAAGGACT CATCCTGCAC 
TACTACTCAG AGAGAGAAGG GCTGCAGGAT ATTGTCATCG GAATCATCAA GACAGTAGCT 
CAGCAGATAC ATGGCACTGA AATTGACATG AAGGTTATTC AACAAAGAAA TGAAGAATGT 
GATCATACTC AATTTTTGAT TGAAGAAAAA GAGTCAAAAG AAGAGGATTT TTATGAAGAT 
CTTGACAGAT TTGAAGAAAA CGGTACCCAG GAATCACGCA TCAGCCCATA TACGTTCTGC 
AAAGCCTTTC CCTTTCACAT CATATTTGAC AGGGATCTTG TGGTCACTCA GTGTGGCAAC 
GCCATCTACA GAGTCCTTCC CCAGCTCCAG CCTGGGAACT GCAGCCTCCT GTCTGTCTTC 
TCCCTGGTCC GTCCTCATAT TGACATCAGT TTCCATGGAA TCCTCTCACA CATCAATACA 
GTCTTCGTGT TGAGAAGCAA GGAAGGGTTG TTGGATGTAG AGAAATCAGA GTGTGAGGAT 
GAGCTGACGG GCACTGAAAT CAGCTGCTTA CGGCTCAAGG GTCAGATGAT CTACTTGCCC 
GAAGCAGACA GCATACTTTT CCTGTGCTCA CCAAGTGTCA TGAACCTGGA CGACCTGACC 
CGGCGAGGGC TGTACCTGAG TGACATCCCT CTGCACGATG CCACTCGGGA CCTCGTGCTC 
CTGGGAGAGC AGTTCAGAGA GGAGTACAAG CTGACCCAGG AACTGGAAAT CCTCACAGAC 
AGGCTGCAGC TCACGTTACG AGCCCTGGAA GATGAAAAGA AAAAGACGGA CACGTTGCTG 
TATTCTGTGC TTCCTCCATC TGTGGCCAAC GAGCTGAGGC ACAAGCGTCC GGTGCCTGCG 
AAGAGATATG ACAACGTGAC TATCCTCTTC AGCGGCATTG TGGGCTTCAA TGCTTTCTGC 
AGCAAGCACG CGTCTGGGGA AGGGGCCATG AAGATTGTCA ACCTCCTCAA TGACCTCTAC 
ACTAGATTTG ACACTCTGAC GGACTCCCGG AAAAATCCAT TTGTTTATAA GGTGGAGACT 
GTCGGGGACA AGTACATGAC AGTGAGCGGG CTGCCGGAGC CATGCATCCA CCACGCACGG 
TCCATCTGCC ACCTGGCCTT GGATATGATG GAGATTGCTG GCCAGGTCCA AGTAGATGGT 
GAATCTGTTC AGATAACGAT CGGGATCCAC ACAGGGGAGG TAGTGACCGG AGTCATAGGA 
CAGAGGATGC CTCGCTACTG TCTTTTTGGA AACACTGTCA ACCTCACAAG CCGAACAGAA 
ACCACTGGAG AAAAAGGGAA AATAAATGTG TCTGAATATA CATACAGATG TCTCATGACA 
CCAGAAAATT CAGACCCGCA ATTCCATTTG GAGCACAGAG GCCCCGTGTC CATGAAGGGC 
AAAAAGGAGC CAATGCAAGT TTGGTTTCTA TCCAGAAAAA ATACAGGAAC AGAGGAAACG 
GAGCAGGATG AAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832795.1
CDS	171..1688
Translation	MRAPSFRCTDADKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKSECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMTPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETEQDEN

Target ORF information:

RefSeq Version	XM_024977027.1
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977027.1

ORF Insert Sequence:

ATGCGGGCAC CTTCCTTCCG GTGCACTGAT GCGGACAAGG GCAAAGGACT CATCCTGCAC 
TACTACTCAG AGAGAGAAGG GCTGCAGGAT ATTGTCATCG GAATCATCAA GACAGTAGCT 
CAGCAGATAC ATGGCACTGA AATTGACATG AAGGTTATTC AACAAAGAAA TGAAGAATGT 
GATCATACTC AATTTTTGAT TGAAGAAAAA GAGTCAAAAG AAGAGGATTT TTATGAAGAT 
CTTGACAGAT TTGAAGAAAA CGGTACCCAG GAATCACGCA TCAGCCCATA TACGTTCTGC 
AAAGCCTTTC CCTTTCACAT CATATTTGAC AGGGATCTTG TGGTCACTCA GTGTGGCAAC 
GCCATCTACA GAGTCCTTCC CCAGCTCCAG CCTGGGAACT GCAGCCTCCT GTCTGTCTTC 
TCCCTGGTCC GTCCTCATAT TGACATCAGT TTCCATGGAA TCCTCTCACA CATCAATACA 
GTCTTCGTGT TGAGAAGCAA GGAAGGGTTG TTGGATGTAG AGAAATCAGA GTGTGAGGAT 
GAGCTGACGG GCACTGAAAT CAGCTGCTTA CGGCTCAAGG GTCAGATGAT CTACTTGCCC 
GAAGCAGACA GCATACTTTT CCTGTGCTCA CCAAGTGTCA TGAACCTGGA CGACCTGACC 
CGGCGAGGGC TGTACCTGAG TGACATCCCT CTGCACGATG CCACTCGGGA CCTCGTGCTC 
CTGGGAGAGC AGTTCAGAGA GGAGTACAAG CTGACCCAGG AACTGGAAAT CCTCACAGAC 
AGGCTGCAGC TCACGTTACG AGCCCTGGAA GATGAAAAGA AAAAGACGGA CACGTTGCTG 
TATTCTGTGC TTCCTCCATC TGTGGCCAAC GAGCTGAGGC ACAAGCGTCC GGTGCCTGCG 
AAGAGATATG ACAACGTGAC TATCCTCTTC AGCGGCATTG TGGGCTTCAA TGCTTTCTGC 
AGCAAGCACG CGTCTGGGGA AGGGGCCATG AAGATTGTCA ACCTCCTCAA TGACCTCTAC 
ACTAGATTTG ACACTCTGAC GGACTCCCGG AAAAATCCAT TTGTTTATAA GGTGGAGACT 
GTCGGGGACA AGTACATGAC AGTGAGCGGG CTGCCGGAGC CATGCATCCA CCACGCACGG 
TCCATCTGCC ACCTGGCCTT GGATATGATG GAGATTGCTG GCCAGGTCCA AGTAGATGGT 
GAATCTGTTC AGATAACGAT CGGGATCCAC ACAGGGGAGG TAGTGACCGG AGTCATAGGA 
CAGAGGATGC CTCGCTACTG TCTTTTTGGA AACACTGTCA ACCTCACAAG CCGAACAGAA 
ACCACTGGAG AAAAAGGGAA AATAAATGTG TCTGAATATA CATACAGATG TCTCATGACA 
CCAGAAAATT CAGACCCGCA ATTCCATTTG GAGCACAGAG GCCCCGTGTC CATGAAGGGC 
AAAAAGGAGC CAATGCAAGT TTGGTTTCTA TCCAGAAAAA ATACAGGAAC AGAGGAAACG 
GAGCAGGATG AAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heat Shock Protein 90 Associates with the Per-Arnt-Sim Domain of Heme-free Soluble Guanylate Cyclase: IMplications for Enzyme Maturation.
The Journal of biological chemistry290(35)21615-28(2015 Aug)
Sarkar A,Dai Y,Haque MM,Seeger F,Ghosh A,Garcin ED,Montfort WR,Hazen SL,Misra S,Stuehr DJ

Quaternary structure controls ligand dynamics in soluble guanylate cyclase.
The Journal of biological chemistry287(9)6851-9(2012 Feb)
Yoo BK,Lamarre I,Martin JL,Negrerie M

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Dimerization of nitric oxide-sensitive guanylyl cyclase requires the alpha 1 N terminus.
The Journal of biological chemistry280(18)17687-93(2005 May)
Wagner C,Russwurm M,J?ger R,Friebe A,Koesling D

Identification of histidine 105 in the beta1 subunit of soluble guanylate cyclase as the heme proximal ligand.
Biochemistry37(13)4502-9(1998 Mar)
Zhao Y,Schelvis JP,Babcock GT,Marletta MA

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

GUCY1B1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム