GUCY1B1 cDNA ORF clone, Homo sapiens(human)

The following GUCY1B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY1B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24495 NM_000857.4
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu24504 NM_001291955.2
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu24504 XM_017008133.1
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu24504 XM_017008132.1
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu24524 NM_001291954.2
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24531 NM_001291953.2
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24557 NM_001291952.2
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24561 NM_001291951.2
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24531 XM_017008131.2
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24531 XM_017008130.1
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu58163 XM_011531901.1
Latest version!
Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
View Old Accession Versions >>
OHu24495 NM_000857.3 Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu24561 NM_001291951.1 Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24531 XM_017008131.1 Homo sapiens guanylate cyclase 1, soluble, beta 3 (GUCY1B3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24557 NM_001291952.1 Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24504 NM_001291955.1 Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu24524 NM_001291954.1 Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu24531 NM_001291953.1 Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu24495
    Clone ID Related Accession (Same CDS sequence) NM_000857.3 , NM_000857.4
    Accession Version NM_000857.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, X66533.1, BC026332.1, AI189698.1 and DB536632.1. On May 14, 2014 this sequence version replaced NM_000857.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.21718.1, SRR1803614.206281.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACATTGC TGTATTCTGT CCTTCCTCCG
    TCTGTTGCCA ATGAGCTGCG GCACAAGCGT CCAGTGCCTG CCAAAAGATA TGACAATGTG
    ACCATCCTCT TTAGTGGCAT TGTGGGCTTC AATGCTTTCT GTAGCAAGCA TGCATCTGGA
    GAAGGAGCCA TGAAGATCGT CAACCTCCTC AACGACCTCT ACACCAGATT TGACACACTG
    ACTGATTCCC GGAAAAACCC ATTTGTTTAT AAGGTGGAGA CTGTTGGTGA CAAGTATATG
    ACAGTGAGTG GTTTACCAGA GCCATGCATT CACCATGCAC GATCCATCTG CCACCTGGCC
    TTGGACATGA TGGAAATTGC TGGCCAGGTT CAAGTAGATG GTGAATCTGT TCAGATAACA
    ATAGGGATAC ACACTGGAGA GGTAGTTACA GGTGTCATAG GACAGCGGAT GCCTCGATAC
    TGTCTTTTTG GGAATACTGT CAACCTCACA AGCCGAACAG AAACCACAGG AGAAAAGGGA
    AAAATAAATG TGTCTGAATA TACATACAGA TGTCTTATGT CTCCAGAAAA TTCAGATCCA
    CAATTCCACT TGGAGCACAG AGGCCCAGTG TCCATGAAGG GCAAAAAAGA ACCAATGCAA
    GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CAAAGCAGGA TGATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000848.1
    CDS169..2028
    Translation

    Target ORF information:

    RefSeq Version NM_000857.3
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000857.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACATTGC TGTATTCTGT CCTTCCTCCG
    TCTGTTGCCA ATGAGCTGCG GCACAAGCGT CCAGTGCCTG CCAAAAGATA TGACAATGTG
    ACCATCCTCT TTAGTGGCAT TGTGGGCTTC AATGCTTTCT GTAGCAAGCA TGCATCTGGA
    GAAGGAGCCA TGAAGATCGT CAACCTCCTC AACGACCTCT ACACCAGATT TGACACACTG
    ACTGATTCCC GGAAAAACCC ATTTGTTTAT AAGGTGGAGA CTGTTGGTGA CAAGTATATG
    ACAGTGAGTG GTTTACCAGA GCCATGCATT CACCATGCAC GATCCATCTG CCACCTGGCC
    TTGGACATGA TGGAAATTGC TGGCCAGGTT CAAGTAGATG GTGAATCTGT TCAGATAACA
    ATAGGGATAC ACACTGGAGA GGTAGTTACA GGTGTCATAG GACAGCGGAT GCCTCGATAC
    TGTCTTTTTG GGAATACTGT CAACCTCACA AGCCGAACAG AAACCACAGG AGAAAAGGGA
    AAAATAAATG TGTCTGAATA TACATACAGA TGTCTTATGT CTCCAGAAAA TTCAGATCCA
    CAATTCCACT TGGAGCACAG AGGCCCAGTG TCCATGAAGG GCAAAAAAGA ACCAATGCAA
    GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CAAAGCAGGA TGATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24495
    Clone ID Related Accession (Same CDS sequence) NM_000857.3 , NM_000857.4
    Accession Version NM_000857.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, X66533.1, BC026332.1, AI189698.1, DB536632.1 and AC114761.3. On Jun 2, 2019 this sequence version replaced NM_000857.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.130338.1, SRR1660805.134603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACATTGC TGTATTCTGT CCTTCCTCCG
    TCTGTTGCCA ATGAGCTGCG GCACAAGCGT CCAGTGCCTG CCAAAAGATA TGACAATGTG
    ACCATCCTCT TTAGTGGCAT TGTGGGCTTC AATGCTTTCT GTAGCAAGCA TGCATCTGGA
    GAAGGAGCCA TGAAGATCGT CAACCTCCTC AACGACCTCT ACACCAGATT TGACACACTG
    ACTGATTCCC GGAAAAACCC ATTTGTTTAT AAGGTGGAGA CTGTTGGTGA CAAGTATATG
    ACAGTGAGTG GTTTACCAGA GCCATGCATT CACCATGCAC GATCCATCTG CCACCTGGCC
    TTGGACATGA TGGAAATTGC TGGCCAGGTT CAAGTAGATG GTGAATCTGT TCAGATAACA
    ATAGGGATAC ACACTGGAGA GGTAGTTACA GGTGTCATAG GACAGCGGAT GCCTCGATAC
    TGTCTTTTTG GGAATACTGT CAACCTCACA AGCCGAACAG AAACCACAGG AGAAAAGGGA
    AAAATAAATG TGTCTGAATA TACATACAGA TGTCTTATGT CTCCAGAAAA TTCAGATCCA
    CAATTCCACT TGGAGCACAG AGGCCCAGTG TCCATGAAGG GCAAAAAAGA ACCAATGCAA
    GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CAAAGCAGGA TGATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000848.1
    CDS121..1980
    Translation

    Target ORF information:

    RefSeq Version NM_000857.4
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000857.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACATTGC TGTATTCTGT CCTTCCTCCG
    TCTGTTGCCA ATGAGCTGCG GCACAAGCGT CCAGTGCCTG CCAAAAGATA TGACAATGTG
    ACCATCCTCT TTAGTGGCAT TGTGGGCTTC AATGCTTTCT GTAGCAAGCA TGCATCTGGA
    GAAGGAGCCA TGAAGATCGT CAACCTCCTC AACGACCTCT ACACCAGATT TGACACACTG
    ACTGATTCCC GGAAAAACCC ATTTGTTTAT AAGGTGGAGA CTGTTGGTGA CAAGTATATG
    ACAGTGAGTG GTTTACCAGA GCCATGCATT CACCATGCAC GATCCATCTG CCACCTGGCC
    TTGGACATGA TGGAAATTGC TGGCCAGGTT CAAGTAGATG GTGAATCTGT TCAGATAACA
    ATAGGGATAC ACACTGGAGA GGTAGTTACA GGTGTCATAG GACAGCGGAT GCCTCGATAC
    TGTCTTTTTG GGAATACTGT CAACCTCACA AGCCGAACAG AAACCACAGG AGAAAAGGGA
    AAAATAAATG TGTCTGAATA TACATACAGA TGTCTTATGT CTCCAGAAAA TTCAGATCCA
    CAATTCCACT TGGAGCACAG AGGCCCAGTG TCCATGAAGG GCAAAAAAGA ACCAATGCAA
    GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CAAAGCAGGA TGATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24504
    Clone ID Related Accession (Same CDS sequence) NM_001291955.1 , NM_001291955.2 , XM_017008132.1 , XM_017008133.1
    Accession Version NM_001291955.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 6
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, AK315944.1, BC026332.1, AI189698.1 and DB536632.1. Transcript Variant: This variant (6) differs in the 5' UTR and 5' coding region, initiates translation at a downstream start codon and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 6, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.117566.1, SRR1660805.33167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CATTGCTGTA TTCTGTCCTT CCTCCGTCTG TTGCCAATGA GCTGCGGCAC
    AAGCGTCCAG TGCCTGCCAA AAGATATGAC AATGTGACCA TCCTCTTTAG TGGCATTGTG
    GGCTTCAATG CTTTCTGTAG CAAGCATGCA TCTGGAGAAG GAGCCATGAA GATCGTCAAC
    CTCCTCAACG ACCTCTACAC CAGATTTGAC ACACTGACTG ATTCCCGGAA AAACCCATTT
    GTTTATAAGG TGGAGACTGT TGGTGACAAG TATATGACAG TGAGTGGTTT ACCAGAGCCA
    TGCATTCACC ATGCACGATC CATCTGCCAC CTGGCCTTGG ACATGATGGA AATTGCTGGC
    CAGGTTCAAG TAGATGGTGA ATCTGTTCAG ATAACAATAG GGATACACAC TGGAGAGGTA
    GTTACAGGTG TCATAGGACA GCGGATGCCT CGATACTGTC TTTTTGGGAA TACTGTCAAC
    CTCACAAGCC GAACAGAAAC CACAGGAGAA AAGGGAAAAA TAAATGTGTC TGAATATACA
    TACAGATGTC TTATGTCTCC AGAAAATTCA GATCCACAAT TCCACTTGGA GCACAGAGGC
    CCAGTGTCCA TGAAGGGCAA AAAAGAACCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT
    ACAGGAACAG AGGAAACAAA GCAGGATGAT GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278884.1
    CDS299..1954
    Translation

    Target ORF information:

    RefSeq Version NM_001291955.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291955.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CATTGCTGTA TTCTGTCCTT CCTCCGTCTG TTGCCAATGA GCTGCGGCAC
    AAGCGTCCAG TGCCTGCCAA AAGATATGAC AATGTGACCA TCCTCTTTAG TGGCATTGTG
    GGCTTCAATG CTTTCTGTAG CAAGCATGCA TCTGGAGAAG GAGCCATGAA GATCGTCAAC
    CTCCTCAACG ACCTCTACAC CAGATTTGAC ACACTGACTG ATTCCCGGAA AAACCCATTT
    GTTTATAAGG TGGAGACTGT TGGTGACAAG TATATGACAG TGAGTGGTTT ACCAGAGCCA
    TGCATTCACC ATGCACGATC CATCTGCCAC CTGGCCTTGG ACATGATGGA AATTGCTGGC
    CAGGTTCAAG TAGATGGTGA ATCTGTTCAG ATAACAATAG GGATACACAC TGGAGAGGTA
    GTTACAGGTG TCATAGGACA GCGGATGCCT CGATACTGTC TTTTTGGGAA TACTGTCAAC
    CTCACAAGCC GAACAGAAAC CACAGGAGAA AAGGGAAAAA TAAATGTGTC TGAATATACA
    TACAGATGTC TTATGTCTCC AGAAAATTCA GATCCACAAT TCCACTTGGA GCACAGAGGC
    CCAGTGTCCA TGAAGGGCAA AAAAGAACCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT
    ACAGGAACAG AGGAAACAAA GCAGGATGAT GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24504
    Clone ID Related Accession (Same CDS sequence) NM_001291955.1 , NM_001291955.2 , XM_017008132.1 , XM_017008133.1
    Accession Version NM_001291955.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 6
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK315944.1, BC026332.1, AI189698.1, DB536632.1 and AC114761.3. On Jun 2, 2019 this sequence version replaced NM_001291955.1. Transcript Variant: This variant (6) differs in the 5' UTR and 5' coding region, initiates translation at a downstream start codon and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 6, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.153613.1, SRR1803616.241073.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CATTGCTGTA TTCTGTCCTT CCTCCGTCTG TTGCCAATGA GCTGCGGCAC
    AAGCGTCCAG TGCCTGCCAA AAGATATGAC AATGTGACCA TCCTCTTTAG TGGCATTGTG
    GGCTTCAATG CTTTCTGTAG CAAGCATGCA TCTGGAGAAG GAGCCATGAA GATCGTCAAC
    CTCCTCAACG ACCTCTACAC CAGATTTGAC ACACTGACTG ATTCCCGGAA AAACCCATTT
    GTTTATAAGG TGGAGACTGT TGGTGACAAG TATATGACAG TGAGTGGTTT ACCAGAGCCA
    TGCATTCACC ATGCACGATC CATCTGCCAC CTGGCCTTGG ACATGATGGA AATTGCTGGC
    CAGGTTCAAG TAGATGGTGA ATCTGTTCAG ATAACAATAG GGATACACAC TGGAGAGGTA
    GTTACAGGTG TCATAGGACA GCGGATGCCT CGATACTGTC TTTTTGGGAA TACTGTCAAC
    CTCACAAGCC GAACAGAAAC CACAGGAGAA AAGGGAAAAA TAAATGTGTC TGAATATACA
    TACAGATGTC TTATGTCTCC AGAAAATTCA GATCCACAAT TCCACTTGGA GCACAGAGGC
    CCAGTGTCCA TGAAGGGCAA AAAAGAACCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT
    ACAGGAACAG AGGAAACAAA GCAGGATGAT GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278884.1
    CDS251..1906
    Translation

    Target ORF information:

    RefSeq Version NM_001291955.2
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291955.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CATTGCTGTA TTCTGTCCTT CCTCCGTCTG TTGCCAATGA GCTGCGGCAC
    AAGCGTCCAG TGCCTGCCAA AAGATATGAC AATGTGACCA TCCTCTTTAG TGGCATTGTG
    GGCTTCAATG CTTTCTGTAG CAAGCATGCA TCTGGAGAAG GAGCCATGAA GATCGTCAAC
    CTCCTCAACG ACCTCTACAC CAGATTTGAC ACACTGACTG ATTCCCGGAA AAACCCATTT
    GTTTATAAGG TGGAGACTGT TGGTGACAAG TATATGACAG TGAGTGGTTT ACCAGAGCCA
    TGCATTCACC ATGCACGATC CATCTGCCAC CTGGCCTTGG ACATGATGGA AATTGCTGGC
    CAGGTTCAAG TAGATGGTGA ATCTGTTCAG ATAACAATAG GGATACACAC TGGAGAGGTA
    GTTACAGGTG TCATAGGACA GCGGATGCCT CGATACTGTC TTTTTGGGAA TACTGTCAAC
    CTCACAAGCC GAACAGAAAC CACAGGAGAA AAGGGAAAAA TAAATGTGTC TGAATATACA
    TACAGATGTC TTATGTCTCC AGAAAATTCA GATCCACAAT TCCACTTGGA GCACAGAGGC
    CCAGTGTCCA TGAAGGGCAA AAAAGAACCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT
    ACAGGAACAG AGGAAACAAA GCAGGATGAT GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24504
    Clone ID Related Accession (Same CDS sequence) NM_001291955.1 , NM_001291955.2 , XM_017008132.1 , XM_017008133.1
    Accession Version XM_017008132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CATTGCTGTA TTCTGTCCTT CCTCCGTCTG TTGCCAATGA GCTGCGGCAC
    AAGCGTCCAG TGCCTGCCAA AAGATATGAC AATGTGACCA TCCTCTTTAG TGGCATTGTG
    GGCTTCAATG CTTTCTGTAG CAAGCATGCA TCTGGAGAAG GAGCCATGAA GATCGTCAAC
    CTCCTCAACG ACCTCTACAC CAGATTTGAC ACACTGACTG ATTCCCGGAA AAACCCATTT
    GTTTATAAGG TGGAGACTGT TGGTGACAAG TATATGACAG TGAGTGGTTT ACCAGAGCCA
    TGCATTCACC ATGCACGATC CATCTGCCAC CTGGCCTTGG ACATGATGGA AATTGCTGGC
    CAGGTTCAAG TAGATGGTGA ATCTGTTCAG ATAACAATAG GGATACACAC TGGAGAGGTA
    GTTACAGGTG TCATAGGACA GCGGATGCCT CGATACTGTC TTTTTGGGAA TACTGTCAAC
    CTCACAAGCC GAACAGAAAC CACAGGAGAA AAGGGAAAAA TAAATGTGTC TGAATATACA
    TACAGATGTC TTATGTCTCC AGAAAATTCA GATCCACAAT TCCACTTGGA GCACAGAGGC
    CCAGTGTCCA TGAAGGGCAA AAAAGAACCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT
    ACAGGAACAG AGGAAACAAA GCAGGATGAT GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863621.1
    CDS564..2219
    Translation

    Target ORF information:

    RefSeq Version XM_017008132.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CATTGCTGTA TTCTGTCCTT CCTCCGTCTG TTGCCAATGA GCTGCGGCAC
    AAGCGTCCAG TGCCTGCCAA AAGATATGAC AATGTGACCA TCCTCTTTAG TGGCATTGTG
    GGCTTCAATG CTTTCTGTAG CAAGCATGCA TCTGGAGAAG GAGCCATGAA GATCGTCAAC
    CTCCTCAACG ACCTCTACAC CAGATTTGAC ACACTGACTG ATTCCCGGAA AAACCCATTT
    GTTTATAAGG TGGAGACTGT TGGTGACAAG TATATGACAG TGAGTGGTTT ACCAGAGCCA
    TGCATTCACC ATGCACGATC CATCTGCCAC CTGGCCTTGG ACATGATGGA AATTGCTGGC
    CAGGTTCAAG TAGATGGTGA ATCTGTTCAG ATAACAATAG GGATACACAC TGGAGAGGTA
    GTTACAGGTG TCATAGGACA GCGGATGCCT CGATACTGTC TTTTTGGGAA TACTGTCAAC
    CTCACAAGCC GAACAGAAAC CACAGGAGAA AAGGGAAAAA TAAATGTGTC TGAATATACA
    TACAGATGTC TTATGTCTCC AGAAAATTCA GATCCACAAT TCCACTTGGA GCACAGAGGC
    CCAGTGTCCA TGAAGGGCAA AAAAGAACCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT
    ACAGGAACAG AGGAAACAAA GCAGGATGAT GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24504
    Clone ID Related Accession (Same CDS sequence) NM_001291955.1 , NM_001291955.2 , XM_017008132.1 , XM_017008133.1
    Accession Version XM_017008133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CATTGCTGTA TTCTGTCCTT CCTCCGTCTG TTGCCAATGA GCTGCGGCAC
    AAGCGTCCAG TGCCTGCCAA AAGATATGAC AATGTGACCA TCCTCTTTAG TGGCATTGTG
    GGCTTCAATG CTTTCTGTAG CAAGCATGCA TCTGGAGAAG GAGCCATGAA GATCGTCAAC
    CTCCTCAACG ACCTCTACAC CAGATTTGAC ACACTGACTG ATTCCCGGAA AAACCCATTT
    GTTTATAAGG TGGAGACTGT TGGTGACAAG TATATGACAG TGAGTGGTTT ACCAGAGCCA
    TGCATTCACC ATGCACGATC CATCTGCCAC CTGGCCTTGG ACATGATGGA AATTGCTGGC
    CAGGTTCAAG TAGATGGTGA ATCTGTTCAG ATAACAATAG GGATACACAC TGGAGAGGTA
    GTTACAGGTG TCATAGGACA GCGGATGCCT CGATACTGTC TTTTTGGGAA TACTGTCAAC
    CTCACAAGCC GAACAGAAAC CACAGGAGAA AAGGGAAAAA TAAATGTGTC TGAATATACA
    TACAGATGTC TTATGTCTCC AGAAAATTCA GATCCACAAT TCCACTTGGA GCACAGAGGC
    CCAGTGTCCA TGAAGGGCAA AAAAGAACCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT
    ACAGGAACAG AGGAAACAAA GCAGGATGAT GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863622.1
    CDS351..2006
    Translation

    Target ORF information:

    RefSeq Version XM_017008133.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CATTGCTGTA TTCTGTCCTT CCTCCGTCTG TTGCCAATGA GCTGCGGCAC
    AAGCGTCCAG TGCCTGCCAA AAGATATGAC AATGTGACCA TCCTCTTTAG TGGCATTGTG
    GGCTTCAATG CTTTCTGTAG CAAGCATGCA TCTGGAGAAG GAGCCATGAA GATCGTCAAC
    CTCCTCAACG ACCTCTACAC CAGATTTGAC ACACTGACTG ATTCCCGGAA AAACCCATTT
    GTTTATAAGG TGGAGACTGT TGGTGACAAG TATATGACAG TGAGTGGTTT ACCAGAGCCA
    TGCATTCACC ATGCACGATC CATCTGCCAC CTGGCCTTGG ACATGATGGA AATTGCTGGC
    CAGGTTCAAG TAGATGGTGA ATCTGTTCAG ATAACAATAG GGATACACAC TGGAGAGGTA
    GTTACAGGTG TCATAGGACA GCGGATGCCT CGATACTGTC TTTTTGGGAA TACTGTCAAC
    CTCACAAGCC GAACAGAAAC CACAGGAGAA AAGGGAAAAA TAAATGTGTC TGAATATACA
    TACAGATGTC TTATGTCTCC AGAAAATTCA GATCCACAAT TCCACTTGGA GCACAGAGGC
    CCAGTGTCCA TGAAGGGCAA AAAAGAACCA ATGCAAGTTT GGTTTCTATC CAGAAAAAAT
    ACAGGAACAG AGGAAACAAA GCAGGATGAT GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24524
    Clone ID Related Accession (Same CDS sequence) NM_001291954.1 , NM_001291954.2
    Accession Version NM_001291954.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, AF020340.1, BC026332.1, AI189698.1 and DB536632.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region and contains an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 5, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.83352.1, SRR1660805.159191.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACTGGCA TTGTGGGCTT CAATGCTTTC
    TGTAGCAAGC ATGCATCTGG AGAAGGAGCC ATGAAGATCG TCAACCTCCT CAACGACCTC
    TACACCAGAT TTGACACACT GACTGATTCC CGGAAAAACC CATTTGTTTA TAAGGTGGAG
    ACTGTTGGTG ACAAGTATAT GACAGTGAGT GGTTTACCAG AGCCATGCAT TCACCATGCA
    CGATCCATCT GCCACCTGGC CTTGGACATG ATGGAAATTG CTGGCCAGGT TCAAGTAGAT
    GGTGAATCTG TTCAGATAAC AATAGGGATA CACACTGGAG AGGTAGTTAC AGGTGTCATA
    GGACAGCGGA TGCCTCGATA CTGTCTTTTT GGGAATACTG TCAACCTCAC AAGCCGAACA
    GAAACCACAG GAGAAAAGGG AAAAATAAAT GTGTCTGAAT ATACATACAG ATGTCTTATG
    TCTCCAGAAA ATTCAGATCC ACAATTCCAC TTGGAGCACA GAGGCCCAGT GTCCATGAAG
    GGCAAAAAAG AACCAATGCA AGTTTGGTTT CTATCCAGAA AAAATACAGG AACAGAGGAA
    ACAAAGCAGG ATGATGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278883.1
    CDS169..1929
    Translation

    Target ORF information:

    RefSeq Version NM_001291954.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291954.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACTGGCA TTGTGGGCTT CAATGCTTTC
    TGTAGCAAGC ATGCATCTGG AGAAGGAGCC ATGAAGATCG TCAACCTCCT CAACGACCTC
    TACACCAGAT TTGACACACT GACTGATTCC CGGAAAAACC CATTTGTTTA TAAGGTGGAG
    ACTGTTGGTG ACAAGTATAT GACAGTGAGT GGTTTACCAG AGCCATGCAT TCACCATGCA
    CGATCCATCT GCCACCTGGC CTTGGACATG ATGGAAATTG CTGGCCAGGT TCAAGTAGAT
    GGTGAATCTG TTCAGATAAC AATAGGGATA CACACTGGAG AGGTAGTTAC AGGTGTCATA
    GGACAGCGGA TGCCTCGATA CTGTCTTTTT GGGAATACTG TCAACCTCAC AAGCCGAACA
    GAAACCACAG GAGAAAAGGG AAAAATAAAT GTGTCTGAAT ATACATACAG ATGTCTTATG
    TCTCCAGAAA ATTCAGATCC ACAATTCCAC TTGGAGCACA GAGGCCCAGT GTCCATGAAG
    GGCAAAAAAG AACCAATGCA AGTTTGGTTT CTATCCAGAA AAAATACAGG AACAGAGGAA
    ACAAAGCAGG ATGATGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24524
    Clone ID Related Accession (Same CDS sequence) NM_001291954.1 , NM_001291954.2
    Accession Version NM_001291954.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-08
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, AF020340.1, BC026332.1, AI189698.1, DB536632.1 and AC114761.3. On Jun 2, 2019 this sequence version replaced NM_001291954.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region and contains an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 5, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.83352.1, SRR1803616.128662.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACTGGCA TTGTGGGCTT CAATGCTTTC
    TGTAGCAAGC ATGCATCTGG AGAAGGAGCC ATGAAGATCG TCAACCTCCT CAACGACCTC
    TACACCAGAT TTGACACACT GACTGATTCC CGGAAAAACC CATTTGTTTA TAAGGTGGAG
    ACTGTTGGTG ACAAGTATAT GACAGTGAGT GGTTTACCAG AGCCATGCAT TCACCATGCA
    CGATCCATCT GCCACCTGGC CTTGGACATG ATGGAAATTG CTGGCCAGGT TCAAGTAGAT
    GGTGAATCTG TTCAGATAAC AATAGGGATA CACACTGGAG AGGTAGTTAC AGGTGTCATA
    GGACAGCGGA TGCCTCGATA CTGTCTTTTT GGGAATACTG TCAACCTCAC AAGCCGAACA
    GAAACCACAG GAGAAAAGGG AAAAATAAAT GTGTCTGAAT ATACATACAG ATGTCTTATG
    TCTCCAGAAA ATTCAGATCC ACAATTCCAC TTGGAGCACA GAGGCCCAGT GTCCATGAAG
    GGCAAAAAAG AACCAATGCA AGTTTGGTTT CTATCCAGAA AAAATACAGG AACAGAGGAA
    ACAAAGCAGG ATGATGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278883.1
    CDS121..1881
    Translation

    Target ORF information:

    RefSeq Version NM_001291954.2
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291954.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACTGGCA TTGTGGGCTT CAATGCTTTC
    TGTAGCAAGC ATGCATCTGG AGAAGGAGCC ATGAAGATCG TCAACCTCCT CAACGACCTC
    TACACCAGAT TTGACACACT GACTGATTCC CGGAAAAACC CATTTGTTTA TAAGGTGGAG
    ACTGTTGGTG ACAAGTATAT GACAGTGAGT GGTTTACCAG AGCCATGCAT TCACCATGCA
    CGATCCATCT GCCACCTGGC CTTGGACATG ATGGAAATTG CTGGCCAGGT TCAAGTAGAT
    GGTGAATCTG TTCAGATAAC AATAGGGATA CACACTGGAG AGGTAGTTAC AGGTGTCATA
    GGACAGCGGA TGCCTCGATA CTGTCTTTTT GGGAATACTG TCAACCTCAC AAGCCGAACA
    GAAACCACAG GAGAAAAGGG AAAAATAAAT GTGTCTGAAT ATACATACAG ATGTCTTATG
    TCTCCAGAAA ATTCAGATCC ACAATTCCAC TTGGAGCACA GAGGCCCAGT GTCCATGAAG
    GGCAAAAAAG AACCAATGCA AGTTTGGTTT CTATCCAGAA AAAATACAGG AACAGAGGAA
    ACAAAGCAGG ATGATGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24531
    Clone ID Related Accession (Same CDS sequence) NM_001291953.1 , XM_017008131.2 , XM_017008130.1 , XM_017008131.1 , NM_001291953.2
    Accession Version NM_001291953.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, AK307838.1, DC317249.1, AF020340.1, BC026332.1, AI189698.1 and DB536632.1. On May 14, 2014 this sequence version replaced XM_005262959.1. Transcript Variant: This variant (4) differs in the 5' UTR, initiates translation at a downstream start codon and contains multiple coding region differences, compared to variant 1. It encodes isoform 4, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153946, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278882.1
    CDS559..2343
    Translation

    Target ORF information:

    RefSeq Version NM_001291953.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24531
    Clone ID Related Accession (Same CDS sequence) NM_001291953.1 , XM_017008131.2 , XM_017008130.1 , XM_017008131.1 , NM_001291953.2
    Accession Version NM_001291953.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, AK307838.1, DC317249.1, AF020340.1, BC026332.1, AI189698.1, DB536632.1 and AC114761.3. On Jun 2, 2019 this sequence version replaced NM_001291953.1. Transcript Variant: This variant (4) differs in the 5' UTR, initiates translation at a downstream start codon and contains multiple coding region differences, compared to variant 1. It encodes isoform 4, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153946, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278882.1
    CDS511..2295
    Translation

    Target ORF information:

    RefSeq Version NM_001291953.2
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291953.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24557
    Clone ID Related Accession (Same CDS sequence) NM_001291952.2 , NM_001291952.1
    Accession Version NM_001291952.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-08
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, AK296680.1, BC026332.1, AI189698.1, DB536632.1 and AC114761.3. On Jun 2, 2019 this sequence version replaced NM_001291952.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, initiates translation at an alternate start codon and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 3, which is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTTATGT GCTTTATAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA 
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACATTGC TGTATTCTGT CCTTCCTCCG
    TCTGTTGCCA ATGAGCTGCG GCACAAGCGT CCAGTGCCTG CCAAAAGATA TGACAATGTG
    ACCATCCTCT TTAGTGGCAT TGTGGGCTTC AATGCTTTCT GTAGCAAGCA TGCATCTGGA
    GAAGGAGCCA TGAAGATCGT CAACCTCCTC AACGACCTCT ACACCAGATT TGACACACTG
    ACTGATTCCC GGAAAAACCC ATTTGTTTAT AAGGTGGAGA CTGTTGGTGA CAAGTATATG
    ACAGTGAGTG GTTTACCAGA GCCATGCATT CACCATGCAC GATCCATCTG CCACCTGGCC
    TTGGACATGA TGGAAATTGC TGGCCAGGTT CAAGTAGATG GTGAATCTGT TCAGATAACA
    ATAGGGATAC ACACTGGAGA GGTAGTTACA GGTGTCATAG GACAGCGGAT GCCTCGATAC
    TGTCTTTTTG GGAATACTGT CAACCTCACA AGCCGAACAG AAACCACAGG AGAAAAGGGA
    AAAATAAATG TGTCTGAATA TACATACAGA TGTCTTATGT CTCCAGAAAA TTCAGATCCA
    CAATTCCACT TGGAGCACAG AGGCCCAGTG TCCATGAAGG GCAAAAAAGA ACCAATGCAA
    GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CAAAGCAGGA TGATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278881.1
    CDS333..2132
    Translation

    Target ORF information:

    RefSeq Version NM_001291952.2
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291952.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTTATGT GCTTTATAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA 
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACATTGC TGTATTCTGT CCTTCCTCCG
    TCTGTTGCCA ATGAGCTGCG GCACAAGCGT CCAGTGCCTG CCAAAAGATA TGACAATGTG
    ACCATCCTCT TTAGTGGCAT TGTGGGCTTC AATGCTTTCT GTAGCAAGCA TGCATCTGGA
    GAAGGAGCCA TGAAGATCGT CAACCTCCTC AACGACCTCT ACACCAGATT TGACACACTG
    ACTGATTCCC GGAAAAACCC ATTTGTTTAT AAGGTGGAGA CTGTTGGTGA CAAGTATATG
    ACAGTGAGTG GTTTACCAGA GCCATGCATT CACCATGCAC GATCCATCTG CCACCTGGCC
    TTGGACATGA TGGAAATTGC TGGCCAGGTT CAAGTAGATG GTGAATCTGT TCAGATAACA
    ATAGGGATAC ACACTGGAGA GGTAGTTACA GGTGTCATAG GACAGCGGAT GCCTCGATAC
    TGTCTTTTTG GGAATACTGT CAACCTCACA AGCCGAACAG AAACCACAGG AGAAAAGGGA
    AAAATAAATG TGTCTGAATA TACATACAGA TGTCTTATGT CTCCAGAAAA TTCAGATCCA
    CAATTCCACT TGGAGCACAG AGGCCCAGTG TCCATGAAGG GCAAAAAAGA ACCAATGCAA
    GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CAAAGCAGGA TGATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24557
    Clone ID Related Accession (Same CDS sequence) NM_001291952.2 , NM_001291952.1
    Accession Version NM_001291952.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, AK296680.1, BC026332.1, AI189698.1 and DB536632.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, initiates translation at an alternate start codon and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 3, which is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTTATGT GCTTTATAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA 
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACATTGC TGTATTCTGT CCTTCCTCCG
    TCTGTTGCCA ATGAGCTGCG GCACAAGCGT CCAGTGCCTG CCAAAAGATA TGACAATGTG
    ACCATCCTCT TTAGTGGCAT TGTGGGCTTC AATGCTTTCT GTAGCAAGCA TGCATCTGGA
    GAAGGAGCCA TGAAGATCGT CAACCTCCTC AACGACCTCT ACACCAGATT TGACACACTG
    ACTGATTCCC GGAAAAACCC ATTTGTTTAT AAGGTGGAGA CTGTTGGTGA CAAGTATATG
    ACAGTGAGTG GTTTACCAGA GCCATGCATT CACCATGCAC GATCCATCTG CCACCTGGCC
    TTGGACATGA TGGAAATTGC TGGCCAGGTT CAAGTAGATG GTGAATCTGT TCAGATAACA
    ATAGGGATAC ACACTGGAGA GGTAGTTACA GGTGTCATAG GACAGCGGAT GCCTCGATAC
    TGTCTTTTTG GGAATACTGT CAACCTCACA AGCCGAACAG AAACCACAGG AGAAAAGGGA
    AAAATAAATG TGTCTGAATA TACATACAGA TGTCTTATGT CTCCAGAAAA TTCAGATCCA
    CAATTCCACT TGGAGCACAG AGGCCCAGTG TCCATGAAGG GCAAAAAAGA ACCAATGCAA
    GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CAAAGCAGGA TGATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278881.1
    CDS381..2180
    Translation

    Target ORF information:

    RefSeq Version NM_001291952.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291952.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTTATGT GCTTTATAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA 
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACATTGC TGTATTCTGT CCTTCCTCCG
    TCTGTTGCCA ATGAGCTGCG GCACAAGCGT CCAGTGCCTG CCAAAAGATA TGACAATGTG
    ACCATCCTCT TTAGTGGCAT TGTGGGCTTC AATGCTTTCT GTAGCAAGCA TGCATCTGGA
    GAAGGAGCCA TGAAGATCGT CAACCTCCTC AACGACCTCT ACACCAGATT TGACACACTG
    ACTGATTCCC GGAAAAACCC ATTTGTTTAT AAGGTGGAGA CTGTTGGTGA CAAGTATATG
    ACAGTGAGTG GTTTACCAGA GCCATGCATT CACCATGCAC GATCCATCTG CCACCTGGCC
    TTGGACATGA TGGAAATTGC TGGCCAGGTT CAAGTAGATG GTGAATCTGT TCAGATAACA
    ATAGGGATAC ACACTGGAGA GGTAGTTACA GGTGTCATAG GACAGCGGAT GCCTCGATAC
    TGTCTTTTTG GGAATACTGT CAACCTCACA AGCCGAACAG AAACCACAGG AGAAAAGGGA
    AAAATAAATG TGTCTGAATA TACATACAGA TGTCTTATGT CTCCAGAAAA TTCAGATCCA
    CAATTCCACT TGGAGCACAG AGGCCCAGTG TCCATGAAGG GCAAAAAAGA ACCAATGCAA
    GTTTGGTTTC TATCCAGAAA AAATACAGGA ACAGAGGAAA CAAAGCAGGA TGATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24561
    Clone ID Related Accession (Same CDS sequence) NM_001291951.1 , NM_001291951.2
    Accession Version NM_001291951.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC342619.1, AK300296.1, AK315944.1, BC026332.1, AI189698.1 and DB536632.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300296.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAG
    AATGAATCAA AGCATATTTC AGATTTACAT CATTGTTTCC TGGGAATCTA CTTGTGCTGC
    AATAACCTTG ATGCTCTGCA CGACCACCTT GCTACCATCT ACCCAGGAAT GCGTGCACCT
    TCCTTTAGGT GCACTGATGC AGAAAAGGGC AAAGGACTCA TTTTGCACTA CTACTCAGAG
    AGAGAAGGAC TTCAGGATAT TGTCATTGGA ATCATCAAAA CAGTGGCACA ACAAATCCAT
    GGCACTGAAA TAGACATGAA GGTTATTCAG CAAAGAAATG AAGAATGTGA TCATACTCAA
    TTTTTAATTG AAGAAAAAGA GTCAAAAGAA GAGGATTTTT ATGAAGATCT TGACAGATTT
    GAAGAAAATG GTACCCAGGA ATCACGCATC AGCCCATATA CATTCTGCAA AGCTTTTCCT
    TTTCATATAA TATTTGACCG GGACCTAGTG GTCACTCAGT GTGGCAATGC TATATACAGA
    GTTCTCCCCC AGCTCCAGCC TGGGAATTGC AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT
    CCTCATATTG ATATTAGTTT CCATGGGATC CTTTCTCACA TCAATACTGT TTTTGTATTG
    AGAAGCAAGG AAGGATTGTT GGATGTGGAG AAATTAGAAT GTGAGGATGA ACTGACTGGG
    ACTGAGATCA GCTGCTTACG TCTCAAGGGT CAAATGATCT ACTTACCTGA AGCAGATAGC
    ATACTTTTTC TATGTTCACC AAGTGTCATG AACCTGGACG ATTTGACAAG GAGAGGGCTG
    TATCTAAGTG ACATCCCTCT GCATGATGCC ACGCGCGATC TTGTTCTTTT GGGAGAACAA
    TTTAGAGAGG AATACAAACT CACCCAAGAA CTGGAAATCC TCACTGACAG GCTACAGCTC
    ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA AAGACAGACA CATTGCTGTA TTCTGTCCTT
    CCTCCGTCTG TTGCCAATGA GCTGCGGCAC AAGCGTCCAG TGCCTGCCAA AAGATATGAC
    AATGTGACCA TCCTCTTTAG TGGCATTGTG GGCTTCAATG CTTTCTGTAG CAAGCATGCA
    TCTGGAGAAG GAGCCATGAA GATCGTCAAC CTCCTCAACG ACCTCTACAC CAGATTTGAC
    ACACTGACTG ATTCCCGGAA AAACCCATTT GTTTATAAGG TGGAGACTGT TGGTGACAAG
    TATATGACAG TGAGTGGTTT ACCAGAGCCA TGCATTCACC ATGCACGATC CATCTGCCAC
    CTGGCCTTGG ACATGATGGA AATTGCTGGC CAGGTTCAAG TAGATGGTGA ATCTGTTCAG
    ATAACAATAG GGATACACAC TGGAGAGGTA GTTACAGGTG TCATAGGACA GCGGATGCCT
    CGATACTGTC TTTTTGGGAA TACTGTCAAC CTCACAAGCC GAACAGAAAC CACAGGAGAA
    AAGGGAAAAA TAAATGTGTC TGAATATACA TACAGATGTC TTATGTCTCC AGAAAATTCA
    GATCCACAAT TCCACTTGGA GCACAGAGGC CCAGTGTCCA TGAAGGGCAA AAAAGAACCA
    ATGCAAGTTT GGTTTCTATC CAGAAAAAAT ACAGGAACAG AGGAAACAAA GCAGGATGAT
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278880.1
    CDS169..2094
    Translation

    Target ORF information:

    RefSeq Version NM_001291951.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291951.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAG
    AATGAATCAA AGCATATTTC AGATTTACAT CATTGTTTCC TGGGAATCTA CTTGTGCTGC
    AATAACCTTG ATGCTCTGCA CGACCACCTT GCTACCATCT ACCCAGGAAT GCGTGCACCT
    TCCTTTAGGT GCACTGATGC AGAAAAGGGC AAAGGACTCA TTTTGCACTA CTACTCAGAG
    AGAGAAGGAC TTCAGGATAT TGTCATTGGA ATCATCAAAA CAGTGGCACA ACAAATCCAT
    GGCACTGAAA TAGACATGAA GGTTATTCAG CAAAGAAATG AAGAATGTGA TCATACTCAA
    TTTTTAATTG AAGAAAAAGA GTCAAAAGAA GAGGATTTTT ATGAAGATCT TGACAGATTT
    GAAGAAAATG GTACCCAGGA ATCACGCATC AGCCCATATA CATTCTGCAA AGCTTTTCCT
    TTTCATATAA TATTTGACCG GGACCTAGTG GTCACTCAGT GTGGCAATGC TATATACAGA
    GTTCTCCCCC AGCTCCAGCC TGGGAATTGC AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT
    CCTCATATTG ATATTAGTTT CCATGGGATC CTTTCTCACA TCAATACTGT TTTTGTATTG
    AGAAGCAAGG AAGGATTGTT GGATGTGGAG AAATTAGAAT GTGAGGATGA ACTGACTGGG
    ACTGAGATCA GCTGCTTACG TCTCAAGGGT CAAATGATCT ACTTACCTGA AGCAGATAGC
    ATACTTTTTC TATGTTCACC AAGTGTCATG AACCTGGACG ATTTGACAAG GAGAGGGCTG
    TATCTAAGTG ACATCCCTCT GCATGATGCC ACGCGCGATC TTGTTCTTTT GGGAGAACAA
    TTTAGAGAGG AATACAAACT CACCCAAGAA CTGGAAATCC TCACTGACAG GCTACAGCTC
    ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA AAGACAGACA CATTGCTGTA TTCTGTCCTT
    CCTCCGTCTG TTGCCAATGA GCTGCGGCAC AAGCGTCCAG TGCCTGCCAA AAGATATGAC
    AATGTGACCA TCCTCTTTAG TGGCATTGTG GGCTTCAATG CTTTCTGTAG CAAGCATGCA
    TCTGGAGAAG GAGCCATGAA GATCGTCAAC CTCCTCAACG ACCTCTACAC CAGATTTGAC
    ACACTGACTG ATTCCCGGAA AAACCCATTT GTTTATAAGG TGGAGACTGT TGGTGACAAG
    TATATGACAG TGAGTGGTTT ACCAGAGCCA TGCATTCACC ATGCACGATC CATCTGCCAC
    CTGGCCTTGG ACATGATGGA AATTGCTGGC CAGGTTCAAG TAGATGGTGA ATCTGTTCAG
    ATAACAATAG GGATACACAC TGGAGAGGTA GTTACAGGTG TCATAGGACA GCGGATGCCT
    CGATACTGTC TTTTTGGGAA TACTGTCAAC CTCACAAGCC GAACAGAAAC CACAGGAGAA
    AAGGGAAAAA TAAATGTGTC TGAATATACA TACAGATGTC TTATGTCTCC AGAAAATTCA
    GATCCACAAT TCCACTTGGA GCACAGAGGC CCAGTGTCCA TGAAGGGCAA AAAAGAACCA
    ATGCAAGTTT GGTTTCTATC CAGAAAAAAT ACAGGAACAG AGGAAACAAA GCAGGATGAT
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24561
    Clone ID Related Accession (Same CDS sequence) NM_001291951.1 , NM_001291951.2
    Accession Version NM_001291951.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK300296.1, AK315944.1, BC026332.1, AI189698.1, DB536632.1 and AC114761.3. On Jun 2, 2019 this sequence version replaced NM_001291951.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300296.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAG
    AATGAATCAA AGCATATTTC AGATTTACAT CATTGTTTCC TGGGAATCTA CTTGTGCTGC
    AATAACCTTG ATGCTCTGCA CGACCACCTT GCTACCATCT ACCCAGGAAT GCGTGCACCT
    TCCTTTAGGT GCACTGATGC AGAAAAGGGC AAAGGACTCA TTTTGCACTA CTACTCAGAG
    AGAGAAGGAC TTCAGGATAT TGTCATTGGA ATCATCAAAA CAGTGGCACA ACAAATCCAT
    GGCACTGAAA TAGACATGAA GGTTATTCAG CAAAGAAATG AAGAATGTGA TCATACTCAA
    TTTTTAATTG AAGAAAAAGA GTCAAAAGAA GAGGATTTTT ATGAAGATCT TGACAGATTT
    GAAGAAAATG GTACCCAGGA ATCACGCATC AGCCCATATA CATTCTGCAA AGCTTTTCCT
    TTTCATATAA TATTTGACCG GGACCTAGTG GTCACTCAGT GTGGCAATGC TATATACAGA
    GTTCTCCCCC AGCTCCAGCC TGGGAATTGC AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT
    CCTCATATTG ATATTAGTTT CCATGGGATC CTTTCTCACA TCAATACTGT TTTTGTATTG
    AGAAGCAAGG AAGGATTGTT GGATGTGGAG AAATTAGAAT GTGAGGATGA ACTGACTGGG
    ACTGAGATCA GCTGCTTACG TCTCAAGGGT CAAATGATCT ACTTACCTGA AGCAGATAGC
    ATACTTTTTC TATGTTCACC AAGTGTCATG AACCTGGACG ATTTGACAAG GAGAGGGCTG
    TATCTAAGTG ACATCCCTCT GCATGATGCC ACGCGCGATC TTGTTCTTTT GGGAGAACAA
    TTTAGAGAGG AATACAAACT CACCCAAGAA CTGGAAATCC TCACTGACAG GCTACAGCTC
    ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA AAGACAGACA CATTGCTGTA TTCTGTCCTT
    CCTCCGTCTG TTGCCAATGA GCTGCGGCAC AAGCGTCCAG TGCCTGCCAA AAGATATGAC
    AATGTGACCA TCCTCTTTAG TGGCATTGTG GGCTTCAATG CTTTCTGTAG CAAGCATGCA
    TCTGGAGAAG GAGCCATGAA GATCGTCAAC CTCCTCAACG ACCTCTACAC CAGATTTGAC
    ACACTGACTG ATTCCCGGAA AAACCCATTT GTTTATAAGG TGGAGACTGT TGGTGACAAG
    TATATGACAG TGAGTGGTTT ACCAGAGCCA TGCATTCACC ATGCACGATC CATCTGCCAC
    CTGGCCTTGG ACATGATGGA AATTGCTGGC CAGGTTCAAG TAGATGGTGA ATCTGTTCAG
    ATAACAATAG GGATACACAC TGGAGAGGTA GTTACAGGTG TCATAGGACA GCGGATGCCT
    CGATACTGTC TTTTTGGGAA TACTGTCAAC CTCACAAGCC GAACAGAAAC CACAGGAGAA
    AAGGGAAAAA TAAATGTGTC TGAATATACA TACAGATGTC TTATGTCTCC AGAAAATTCA
    GATCCACAAT TCCACTTGGA GCACAGAGGC CCAGTGTCCA TGAAGGGCAA AAAAGAACCA
    ATGCAAGTTT GGTTTCTATC CAGAAAAAAT ACAGGAACAG AGGAAACAAA GCAGGATGAT
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278880.1
    CDS121..2046
    Translation

    Target ORF information:

    RefSeq Version NM_001291951.2
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291951.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAG
    AATGAATCAA AGCATATTTC AGATTTACAT CATTGTTTCC TGGGAATCTA CTTGTGCTGC
    AATAACCTTG ATGCTCTGCA CGACCACCTT GCTACCATCT ACCCAGGAAT GCGTGCACCT
    TCCTTTAGGT GCACTGATGC AGAAAAGGGC AAAGGACTCA TTTTGCACTA CTACTCAGAG
    AGAGAAGGAC TTCAGGATAT TGTCATTGGA ATCATCAAAA CAGTGGCACA ACAAATCCAT
    GGCACTGAAA TAGACATGAA GGTTATTCAG CAAAGAAATG AAGAATGTGA TCATACTCAA
    TTTTTAATTG AAGAAAAAGA GTCAAAAGAA GAGGATTTTT ATGAAGATCT TGACAGATTT
    GAAGAAAATG GTACCCAGGA ATCACGCATC AGCCCATATA CATTCTGCAA AGCTTTTCCT
    TTTCATATAA TATTTGACCG GGACCTAGTG GTCACTCAGT GTGGCAATGC TATATACAGA
    GTTCTCCCCC AGCTCCAGCC TGGGAATTGC AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT
    CCTCATATTG ATATTAGTTT CCATGGGATC CTTTCTCACA TCAATACTGT TTTTGTATTG
    AGAAGCAAGG AAGGATTGTT GGATGTGGAG AAATTAGAAT GTGAGGATGA ACTGACTGGG
    ACTGAGATCA GCTGCTTACG TCTCAAGGGT CAAATGATCT ACTTACCTGA AGCAGATAGC
    ATACTTTTTC TATGTTCACC AAGTGTCATG AACCTGGACG ATTTGACAAG GAGAGGGCTG
    TATCTAAGTG ACATCCCTCT GCATGATGCC ACGCGCGATC TTGTTCTTTT GGGAGAACAA
    TTTAGAGAGG AATACAAACT CACCCAAGAA CTGGAAATCC TCACTGACAG GCTACAGCTC
    ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA AAGACAGACA CATTGCTGTA TTCTGTCCTT
    CCTCCGTCTG TTGCCAATGA GCTGCGGCAC AAGCGTCCAG TGCCTGCCAA AAGATATGAC
    AATGTGACCA TCCTCTTTAG TGGCATTGTG GGCTTCAATG CTTTCTGTAG CAAGCATGCA
    TCTGGAGAAG GAGCCATGAA GATCGTCAAC CTCCTCAACG ACCTCTACAC CAGATTTGAC
    ACACTGACTG ATTCCCGGAA AAACCCATTT GTTTATAAGG TGGAGACTGT TGGTGACAAG
    TATATGACAG TGAGTGGTTT ACCAGAGCCA TGCATTCACC ATGCACGATC CATCTGCCAC
    CTGGCCTTGG ACATGATGGA AATTGCTGGC CAGGTTCAAG TAGATGGTGA ATCTGTTCAG
    ATAACAATAG GGATACACAC TGGAGAGGTA GTTACAGGTG TCATAGGACA GCGGATGCCT
    CGATACTGTC TTTTTGGGAA TACTGTCAAC CTCACAAGCC GAACAGAAAC CACAGGAGAA
    AAGGGAAAAA TAAATGTGTC TGAATATACA TACAGATGTC TTATGTCTCC AGAAAATTCA
    GATCCACAAT TCCACTTGGA GCACAGAGGC CCAGTGTCCA TGAAGGGCAA AAAAGAACCA
    ATGCAAGTTT GGTTTCTATC CAGAAAAAAT ACAGGAACAG AGGAAACAAA GCAGGATGAT
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24531
    Clone ID Related Accession (Same CDS sequence) NM_001291953.1 , XM_017008131.2 , XM_017008130.1 , XM_017008131.1 , NM_001291953.2
    Accession Version XM_017008131.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863620.1
    CDS241..2025
    Translation

    Target ORF information:

    RefSeq Version XM_017008131.2
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008131.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24531
    Clone ID Related Accession (Same CDS sequence) NM_001291953.1 , XM_017008131.2 , XM_017008130.1 , XM_017008131.1 , NM_001291953.2
    Accession Version XM_017008130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863619.1
    CDS564..2348
    Translation

    Target ORF information:

    RefSeq Version XM_017008130.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24531
    Clone ID Related Accession (Same CDS sequence) NM_001291953.1 , XM_017008131.2 , XM_017008130.1 , XM_017008131.1 , NM_001291953.2
    Accession Version XM_017008131.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863620.1
    CDS275..2059
    Translation

    Target ORF information:

    RefSeq Version XM_017008131.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1, soluble, beta 3 (GUCY1B3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTTTGGGA AGATGTTTTT CGTCTTTTGC CAAGAATCTG GTTATGATAC AATCTTGCGT 
    GTCCTGGGCT CTAATGTCAG AGAATTTCTA CAGAACCTTG ATGCTCTGCA CGACCACCTT
    GCTACCATCT ACCCAGGAAT GCGTGCACCT TCCTTTAGGT GCACTGATGC AGAAAAGGGC
    AAAGGACTCA TTTTGCACTA CTACTCAGAG AGAGAAGGAC TTCAGGATAT TGTCATTGGA
    ATCATCAAAA CAGTGGCACA ACAAATCCAT GGCACTGAAA TAGACATGAA GGTTATTCAG
    CAAAGAAATG AAGAATGTGA TCATACTCAA TTTTTAATTG AAGAAAAAGA GTCAAAAGAA
    GAGGATTTTT ATGAAGATCT TGACAGATTT GAAGAAAATG GTACCCAGGA ATCACGCATC
    AGCCCATATA CATTCTGCAA AGCTTTTCCT TTTCATATAA TATTTGACCG GGACCTAGTG
    GTCACTCAGT GTGGCAATGC TATATACAGA GTTCTCCCCC AGCTCCAGCC TGGGAATTGC
    AGCCTTCTGT CTGTCTTCTC GCTGGTTCGT CCTCATATTG ATATTAGTTT CCATGGGATC
    CTTTCTCACA TCAATACTGT TTTTGTATTG AGAAGCAAGG AAGGATTGTT GGATGTGGAG
    AAATTAGAAT GTGAGGATGA ACTGACTGGG ACTGAGATCA GCTGCTTACG TCTCAAGGGT
    CAAATGATCT ACTTACCTGA AGCAGATAGC ATACTTTTTC TATGTTCACC AAGTGTCATG
    AACCTGGACG ATTTGACAAG GAGAGGGCTG TATCTAAGTG ACATCCCTCT GCATGATGCC
    ACGCGCGATC TTGTTCTTTT GGGAGAACAA TTTAGAGAGG AATACAAACT CACCCAAGAA
    CTGGAAATCC TCACTGACAG GCTACAGCTC ACGTTAAGAG CCCTGGAAGA TGAAAAGAAA
    AAGACAGACA CAGGATGTCC TGCTAGAATC CAGGCCTTTA AAGTACAAAC GACACTGATG
    CTGTGTGAAA AGGACAGCAG AAGCACTAAA GGCTTTCCCA GTATTTCTTA CAGTGGCTTT
    CTGCTGATCC CACTGAACAG ATTGCTGTAT TCTGTCCTTC CTCCGTCTGT TGCCAATGAG
    CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA ATGTGACCAT CCTCTTTAGT
    GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT CTGGAGAAGG AGCCATGAAG
    ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA CACTGACTGA TTCCCGGAAA
    AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT ATATGACAGT GAGTGGTTTA
    CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC TGGCCTTGGA CATGATGGAA
    ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA TAACAATAGG GATACACACT
    GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC GATACTGTCT TTTTGGGAAT
    ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA AGGGAAAAAT AAATGTGTCT
    GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG ATCCACAATT CCACTTGGAG
    CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA TGCAAGTTTG GTTTCTATCC
    AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58163
    Clone ID Related Accession (Same CDS sequence) XM_011531901.1
    Accession Version XM_011531901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product guanylate cyclase soluble subunit beta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACAGGAT GTCCTGCTAG AATCCAGGCC
    TTTAAAGTAC AAACGACACT GATGCTGTGT GAAAAGGACA GCAGAAGCAC TAAAGGCTTT
    CCCAGTATTT CTTACAGTGG CTTTCTGCTG ATCCCACTGA ACAGATTGCT GTATTCTGTC
    CTTCCTCCGT CTGTTGCCAA TGAGCTGCGG CACAAGCGTC CAGTGCCTGC CAAAAGATAT
    GACAATGTGA CCATCCTCTT TAGTGGCATT GTGGGCTTCA ATGCTTTCTG TAGCAAGCAT
    GCATCTGGAG AAGGAGCCAT GAAGATCGTC AACCTCCTCA ACGACCTCTA CACCAGATTT
    GACACACTGA CTGATTCCCG GAAAAACCCA TTTGTTTATA AGGTGGAGAC TGTTGGTGAC
    AAGTATATGA CAGTGAGTGG TTTACCAGAG CCATGCATTC ACCATGCACG ATCCATCTGC
    CACCTGGCCT TGGACATGAT GGAAATTGCT GGCCAGGTTC AAGTAGATGG TGAATCTGTT
    CAGATAACAA TAGGGATACA CACTGGAGAG GTAGTTACAG GTGTCATAGG ACAGCGGATG
    CCTCGATACT GTCTTTTTGG GAATACTGTC AACCTCACAA GCCGAACAGA AACCACAGGA
    GAAAAGGGAA AAATAAATGT GTCTGAATAT ACATACAGAT GTCTTATGTC TCCAGAAAAT
    TCAGATCCAC AATTCCACTT GGAGCACAGA GGCCCAGTGT CCATGAAGGG CAAAAAAGAA
    CCAATGCAAG TTTGGTTTCT ATCCAGAAAA AATACAGGAA CAGAGGAAAC AAAGCAGGAT
    GATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530203.1
    CDS83..2071
    Translation

    Target ORF information:

    RefSeq Version XM_011531901.1
    Organism Homo sapiens(human)
    Definition Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTACGGAT TTGTGAATCA CGCCCTGGAG TTGCTGGTGA TCCGCAATTA CGGCCCCGAG 
    GTGTGGGAAG ACATCAAAAA AGAGGCACAG TTAGATGAAG AAGGACAGTT TCTTGTCAGA
    ATAATATATG ATGACTCCAA AACTTATGAT TTGGTTGCTG CTGCAAGCAA AGTCCTCAAT
    CTCAATGCTG GAGAAATCCT CCAAATGTTT GGGAAGATGT TTTTCGTCTT TTGCCAAGAA
    TCTGGTTATG ATACAATCTT GCGTGTCCTG GGCTCTAATG TCAGAGAATT TCTACAGAAC
    CTTGATGCTC TGCACGACCA CCTTGCTACC ATCTACCCAG GAATGCGTGC ACCTTCCTTT
    AGGTGCACTG ATGCAGAAAA GGGCAAAGGA CTCATTTTGC ACTACTACTC AGAGAGAGAA
    GGACTTCAGG ATATTGTCAT TGGAATCATC AAAACAGTGG CACAACAAAT CCATGGCACT
    GAAATAGACA TGAAGGTTAT TCAGCAAAGA AATGAAGAAT GTGATCATAC TCAATTTTTA
    ATTGAAGAAA AAGAGTCAAA AGAAGAGGAT TTTTATGAAG ATCTTGACAG ATTTGAAGAA
    AATGGTACCC AGGAATCACG CATCAGCCCA TATACATTCT GCAAAGCTTT TCCTTTTCAT
    ATAATATTTG ACCGGGACCT AGTGGTCACT CAGTGTGGCA ATGCTATATA CAGAGTTCTC
    CCCCAGCTCC AGCCTGGGAA TTGCAGCCTT CTGTCTGTCT TCTCGCTGGT TCGTCCTCAT
    ATTGATATTA GTTTCCATGG GATCCTTTCT CACATCAATA CTGTTTTTGT ATTGAGAAGC
    AAGGAAGGAT TGTTGGATGT GGAGAAATTA GAATGTGAGG ATGAACTGAC TGGGACTGAG
    ATCAGCTGCT TACGTCTCAA GGGTCAAATG ATCTACTTAC CTGAAGCAGA TAGCATACTT
    TTTCTATGTT CACCAAGTGT CATGAACCTG GACGATTTGA CAAGGAGAGG GCTGTATCTA
    AGTGACATCC CTCTGCATGA TGCCACGCGC GATCTTGTTC TTTTGGGAGA ACAATTTAGA
    GAGGAATACA AACTCACCCA AGAACTGGAA ATCCTCACTG ACAGGCTACA GCTCACGTTA
    AGAGCCCTGG AAGATGAAAA GAAAAAGACA GACACAGGAT GTCCTGCTAG AATCCAGGCC
    TTTAAAGTAC AAACGACACT GATGCTGTGT GAAAAGGACA GCAGAAGCAC TAAAGGCTTT
    CCCAGTATTT CTTACAGTGG CTTTCTGCTG ATCCCACTGA ACAGATTGCT GTATTCTGTC
    CTTCCTCCGT CTGTTGCCAA TGAGCTGCGG CACAAGCGTC CAGTGCCTGC CAAAAGATAT
    GACAATGTGA CCATCCTCTT TAGTGGCATT GTGGGCTTCA ATGCTTTCTG TAGCAAGCAT
    GCATCTGGAG AAGGAGCCAT GAAGATCGTC AACCTCCTCA ACGACCTCTA CACCAGATTT
    GACACACTGA CTGATTCCCG GAAAAACCCA TTTGTTTATA AGGTGGAGAC TGTTGGTGAC
    AAGTATATGA CAGTGAGTGG TTTACCAGAG CCATGCATTC ACCATGCACG ATCCATCTGC
    CACCTGGCCT TGGACATGAT GGAAATTGCT GGCCAGGTTC AAGTAGATGG TGAATCTGTT
    CAGATAACAA TAGGGATACA CACTGGAGAG GTAGTTACAG GTGTCATAGG ACAGCGGATG
    CCTCGATACT GTCTTTTTGG GAATACTGTC AACCTCACAA GCCGAACAGA AACCACAGGA
    GAAAAGGGAA AAATAAATGT GTCTGAATAT ACATACAGAT GTCTTATGTC TCCAGAAAAT
    TCAGATCCAC AATTCCACTT GGAGCACAGA GGCCCAGTGT CCATGAAGGG CAAAAAAGAA
    CCAATGCAAG TTTGGTTTCT ATCCAGAAAA AATACAGGAA CAGAGGAAAC AAAGCAGGAT
    GATGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.